LOCUS       DAWDRC010000001        29084 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_857_length_29084_cov_2.314823, whole genome shotgun sequence.
ACCESSION   DAWDRC010000001 DAWDRC010000000
VERSION     DAWDRC010000001.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29084)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 29084)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_857_length_29084_cov_2.314823"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    231..294
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1742..1751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2964..2973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4202..4211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5169..5178
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7166..7175
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14202..14211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    27781..27790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6545 a         7298 c         7869 g         7238 t
ORIGIN      
        1 tgattatcgg cacgggatgc ggcgcgggcg tggcattcaa tgggcgggcg catatcggcg
       61 gcaatggcac ggcaggcgag tggggacaca atccgctacc gtggatggac gaagacgaac
      121 tgcgttatcg cgaggaagtc ccttgttatt gcggtaaaca aggttgtatt gaaaccttta
      181 tttcaggcac gggattcgcg acggattatc gtcgtttgag cggacatgcg nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctgaaa
      301 ggcagtgaaa ttatccgcct ggttgaagaa agcgatccgg tagcggaact ggcattgcgt
      361 cgctacgagc tgcggctggc aaaatcgctg gcacatgtcg tgaatattct cgatccggat
      421 gtgattgtcc tgggcggcgg aatgagcaat gtagaccgtt tatatcaaac ggttccacag
      481 ttgattaaac aatttgtctt cggcggagaa tgtgaaacgc cggtacgcaa ggcgaaacat
      541 ggtgattcga gcggcgtacg cggcgctgca tggttatggc cacaagagta aaaaaaccag
      601 gcaattggcg tatcatgcct gatgcgacgc ttgccgcgtc ttatcaggcc tacaaagtga
      661 gccgtccggt aggccggata aggcgctcgc gccgcatccg acaacaaata acgcgcacag
      721 tggcggcaac cccccagtgc tccgatgcct gatgcgacac tggcgtgtct tatcaggcct
      781 acaaaatgaa cagtcccgta ggccggataa ggcgttcacg ccgcatccgg caacaagcga
      841 tgcgcactgc gactgctatc taccccagtg gtttcgccag caccgaacta tccgccgctt
      901 gctggcgctt atagcgacca tacagcagca acgacgacat cgcagcaaac gaaagcaggg
      961 cagcgggcag cgccacgtaa ttatatgcca gccccagcgt cagcatcata ccaccgcaat
     1021 atgcgccgac ggcgctaccg aggttaaacg ctatttgccc acctgcggca cctaataact
     1081 ctccgccttt ggcattttgc agtaataata tttgtagagg tgctgatagg gcaaataatc
     1141 ccgcgcaaca aataaaagca aaaataagcg acgttgtttt cataccgccg aagaaaaaga
     1201 gcatcagcag tgccagtaca atgataaagt cagtcactgc tgcaatgcgc agtggtgaat
     1261 aacgtcctga attcctgcca tttagcatat tccccagcac catccctagc ccaactaaca
     1321 tcataataaa ggtcatcgcc gtttccgaaa aaccggaaat aaacatcatg tatggcttta
     1381 cgtagctgaa ccaggcaaac acacctgcgt tgccaaacat cgtagcggcg aaaattaacc
     1441 acggggcagg gctgcgtaaa aaatgaaatt gttcgcgtag ttttcctttc gcctcgtcgc
     1501 gaatatccgg tacccaaaaa tagaccgatg ccatcaccgc aatattaaaa acagcgatca
     1561 acaaaaaggt gtaacgccaa ctaaattcct gacttaaata cgttcccagt ggaatgccca
     1621 gcaaattggc gactgtcatc ccggaaacca tccctgccac ggcggcggtg actttgccgg
     1681 gtttgataat ttttgataac acgatcgcgc cgacgccaaa aaatgcacca tgcggaaagc
     1741 cnnnnnnnnn nagccggaaa ccagccgacc aatggcgagc atcaggtaag acgaagagag
     1801 cgtgaacatg gcgttgccaa tgacgcacaa cgccaccaga aacaacaaga tatgtttgag
     1861 tgagtagcgg ctggaaaaaa gcgcgattat aggcgcaccg accaccaccc ccagcgcata
     1921 atacgagatc atatgcccgc cggcaggaat cgaaattcct acgttatgcg ccagctccgt
     1981 gagcacgccc ataatgccaa attcggccat ccccaaacca aacgtgccca gagccaaaga
     2041 taaaataact tttttcatac caccatcaca accaccagaa cggctaccaa tcaccgtagc
     2101 caataaagtg atagattgca ggataacgac cagcgaggcc aagagataat tcgcccctct
     2161 gtattcatta tcctgctgaa tagttatttc attgcaaacg cactttccag tttgctgtag
     2221 cccagtccat tgatcttctt cactttgatc tgcaccggaa tacgctcttt catcgcctca
     2281 acgtggctaa tcaccccgat ggttttgcca ctggcattca gggcatccag cgcatcaagg
     2341 gcggtatcca gcgtttcgct atccagtgtg ccaaaacctt catcaaggaa cagcgagtca
     2401 atacgcgttt tatggctgac cagatctgaa agcgccagtg ccagcgccag actgacgagg
     2461 aaactttcgc cgccggaaag ggtacgggta tcgcgtaccg catcagcctg ccaggtatca
     2521 acaacctcaa cttccagcgc ttcgctggct ttgcgctgta acagatagcg cccgtgcagc
     2581 cgggtaagtt gctgattagc gagatggact aaattatcca gcgtcagccc ttgggcaaac
     2641 ttgcggaatt tatcgccctc tttggaacct attagcgaat tcagatatcc ccagtcctca
     2701 acctgctgcg tcatttgagc aatttgctgc attaaggttt gttgttgctg acggttatct
     2761 gcatcctgct tcagctgctg gcgaatctcg ccttgactcg tggtgttttc acgcaacttt
     2821 tggtgagttt gcgctaactc ttgctgaatc tgctccgccg tcacagtgag agccaacccg
     2881 ccaggtcggt gttgctgatg ctgtgtcagc gtttctgctg tctgagtgac cagcgtttgc
     2941 gcctgacggc gctggttttc cagnnnnnnn nnntttccag attctgcttg agctgttcca
     3001 gctgcgttag tgtttgttca tccattagcg ccgcaaggaa cgcctgctga tcgtcaaaga
     3061 cgctcgcctg tagcgcggtg tcaaactgcg cctgggcttt ttgcagactt tgcgccgcca
     3121 gaacatcctg ttgctgtaac gtctgctgct ggctgtgtaa tgcgagacat tgttcatgaa
     3181 cctgccgcca gttttccagc gccacagttt cttcgctgtg tgggagttca tcactttgcg
     3241 gcaacgtttc cagaatcggc gtcagctgct gcatacggtt ttgcagcgcg gttaattcgt
     3301 tctggcgttg ctgccagctc tgcgcttctt gctgacgcgt cgccaaccag ctctcttctt
     3361 catcttcctg tggcaatgtc agcgcataac ccgccaatgc cgttaagagt tgctgctggc
     3421 gttgttcaat ttgctgttga tactggataa tttgctggtt atgcgcggca atctgccctt
     3481 gtaattcatg ccgttggctg agtaaccgca gctggcgttc gtgctcatct tgtgcatcta
     3541 gccacggttg aatatcgtcc tgtggctgta gggtgatatt gaggctggcc gtgacggctt
     3601 gccattgttg agtaagtgct tgctcatctt gtcggaggct ttgcgcttcg ttttcatcgc
     3661 gctgaagctg cttcgttaat gcatccagtt gcccacgtag cgccgcacct tcttcgccga
     3721 gctttttcac ttctttttcc agcgttaata gccgggcctg attaacgccc ggttccagcg
     3781 cctgatacgc ctcgaccgcc gggtggctgc tggaaccaca aagtggacaa ggctgacccg
     3841 cctgtaactg tgcgcgctgg gcttccagcg ttttaatgcg cgcttcctgc tcgcaaatgg
     3901 ttttcacatc ggcaagttgt tgcgtttttt ctttataacg atggcgcatt tcgttaagtg
     3961 cggcattacg ttgcgtctgt tccagagtga cattctggat agcgacctgt aactgcgcca
     4021 gacgtttttg ttggggaaca atctgcccat gcagtgcgac caggcgctga cgcagtgggc
     4081 gttgctcagc atgttgcgcc agggcgctag caacttcatc ggcggttaac gtcaacgtga
     4141 tcgccgcaag cgcattaagt ttttgctcag catgagttaa ctgttgctgc cattgccgca
     4201 gnnnnnnnnn nccgcagatg ctcgcgatcg ctggtttgtt gggagaactg tgcacgccaa
     4261 cccgccagtt cgttgttcca ctgacggaag cggtcgtgtt cctgtaacca ggcattcagg
     4321 ctttgttgct gctgctgtaa ttctgctgac tgcttcgcca cgtggtggcg aatgctcgca
     4381 cgaagcgcca ttgtgctctg taagcgagta tttacttctt caatctgctg gcgagtatgc
     4441 gccagcgccg tgctgtgttc tgcgatgcgt tcccagtgcg gacgaagatt tcgtgccggt
     4501 tgtgccagac tgagcgccgc cagttgaggt tgcgcttgtt cttcttcggc taatgcctgt
     4561 tgcaaggcct gttgacggcg gctggcttct tgctgcaatt cgtccagacg cgttaaccag
     4621 tttagcgatt gttgttcttg ctgctgcgcg gtaattaact gtttttcttc gtcagtaagt
     4681 acctgcaaac tcgctgtcag cgattgcact tgttccggcg tgagcaacgc gacgccgctg
     4741 gcctgcgctt gcagcttctc cagctctgtg cgggccgatt tgtgctgctc aaaaaccatc
     4801 gccgagattt gcccgtagat ttcagtgccg gttaactcct cgagcaattc tgcgcgttct
     4861 ttgggtttgg cattcaggaa ggcagcaaat tgcccctgcg aaagcagcat cgaacgggtg
     4921 aagcgcccgt aatccagtcc ggttaacgtc gccgtcagtt ccagcttatc tttcactttg
     4981 tcggcgagaa ttttgccgtc tgcacagcgc gccagctcta cgcgtggcac ctgcaaatta
     5041 ccgtccggct ggttacgcgc ccgattctgg ctccagaacg cgcgatacgc ttcacctttg
     5101 acttcaaact ccacttccgc cagacattcg gcggtatcgc gggtcatgag atcattttgc
     5161 gattgtgann nnnnnnnntt gtgaaacgtt agagaggcgc ggggtttcgt gatacagcgc
     5221 cagacaaatg gcgtccagca gggtggtttt ccccgcaccg gttgggccgg taatagcaaa
     5281 cagcccgttg ctggcgaacg gctcgcgggt gaaatcaatc ttccattcgc cttttaatga
     5341 gttcaggttt ttcaggcgca ggctgagaat tttcatgctt cgtgttctcc ggcgaggcta
     5401 tgcaacgtcg tggcgaaaag atgctgcaga cgttgctgct gtgattcatc cagttcttcc
     5461 agtgccagac ggcgattgaa cacctcttcg acgctgagtt cgctgagggt ttcacgctgt
     5521 tggctggcta acacacgctc gcgctgttca cgactccggc gtaccagcaa tacttcgaca
     5581 ggcaatgatt cggttaatgc ctggattttg cgctgaatat catgcagata ctcatcagta
     5641 gtgatttcga tatccagcca gacaggcggc tcctgtgata catcgcgcca ctgttccagc
     5701 tgtgcggtaa tcgacgccag atcgcctttc agcaccgcca tgggttgcgt taccggtacg
     5761 ttcaggtttt ccacgctctc taatttgccg tttgaaaatg tcaccagatg gacatattta
     5821 ctcttaccgc attcatcaaa actcagtggt atgggggaac cgcaatagcg aacatgttcc
     5881 atgccgccaa taatctgcgc gcggtgaata tgcccgagcg cgatgtagtc ggctggtgga
     5941 aagttttgtg ccggaaacgc gtccagcgtg ccaatataaa tgtcacgcac ggcgtcactt
     6001 ttactggcac ccacggtcgt taaatgtcca gtggtgatga tgggcagagg ctgatcgccg
     6061 cgcagtttgc aggcatcggc ataatgttgt tgataataat cagtaatagc cgccagcaaa
     6121 tgctgctgtt tttcaatacc gttaagcccc gcctggctgg taataatgtc acgcgggcgt
     6181 aaaaaaggta tgggacacag cactgcgcct ggcgtcccgt cgcgacggag caatatttgc
     6241 ggcgcatgtc cggcgctggc gaccacggta gtattgagga acgccatgat atcgcgcgat
     6301 tcattcagcg tggcgaccga gtcatggttt cctgccagta ccaccagatg acagccagtt
     6361 tgctgtaaat tgacaacaaa acggttgtat aacgtgcggg cgtaactggg cggcgagccg
     6421 gtatcgaaaa catcaccggc aacaataatc gcatccacct gatgggcttg tgctgtctcc
     6481 agcagccagt caagaaaagc ctgatgttca gcttcgcggc ttttactgta gaagttctgg
     6541 ccgagatgcc agtctgaggt gtgaaggatg cgcataacgg ttccctggcg aaaaagcatg
     6601 ggcgcgatta tacccaaaca gatgtgccat ttgctttttt ctgtgccacg gaaatcaata
     6661 acctgaaagg atgtgcgacg agcttttcat aaatctgtca taaatctgac gcataatgac
     6721 gtcgcattaa tgatcgcaac ctatttatta caacagggca aatcatggcg agacgtattc
     6781 tggtcgtaga agatgaagct ccaattcgcg aaatggtctg cttcgtgctc gaacaaaatg
     6841 gctttcagcc ggtcgaagcg gaagattatg acagtgctgt gaatcaactg aatgaaccct
     6901 ggccggattt aattctcctc gactggatgt tacctggcgg ctccggtatc cagttcatca
     6961 aacacctcaa gcgcgagtcg atgacccggg atattccagt ggtgatgttg accgccagag
     7021 gggaagaaga agatcgcgtg cgcggccttg aaaccggtgc ggatgactat atcaccaagc
     7081 cgttttctcc gaaggagctg gtggcgcgaa tcaaagcggt aatgcgccgt atttcgccaa
     7141 tggcggtgga agaggtgatc gagatnnnnn nnnnnagggg ctaagtctcg atccgacatc
     7201 tcaccgggtg atggcgggcg aagaaccgct ggagatgggg ccgacagaat ttaaactgct
     7261 gcactttttt atgacgcatc ctgagcgcgt gtacagtcgc gagcaactgt taaatcacgt
     7321 atggggaacc aacgtgtatg tggaagatcg tacggtcgat gtccacattc gtcgcctgcg
     7381 taaagcactg gagcccggcg ggcatgaccg catggtgcag accgtgcgcg gtacagggta
     7441 tcgtttttca acccgctttt aaggccttgc tcatcggacg tagagcaggg cttatgattt
     7501 cttaactgga gtatcttacg tgctggaacg gctgtcgtgg aaaaggctgg tgctggagct
     7561 gctactttgc tgcctcccgg ctttcatcct gggtgcattt tttggttacc tgccctggtt
     7621 tttgctggca tcggtaacag gactgcttat ctggcatttc tggaatttat tgcgcctttc
     7681 atggtggctg tgggtggatc gtagtatgac cccgccaccg gggcgtggta gctgggaacc
     7741 gctactatac ggcttacacc agatgcagct gcgaaataaa aaacgccgcc gtgaactggg
     7801 caatctgatt aaacgctttc gtagcggcgc ggagtcgctg cccgacgcgg tggtgctgac
     7861 cacggaagag ggcggtattt tctggtgcaa cggtctggcg caacagattc ttggcttgcg
     7921 ctggccggaa gataacgggc agaacatcct taacttgctg cgttacccgg agtttacgca
     7981 atatctgaaa acgcgtgatt tctctcgccc gctcaatctg gtgctcaaca ccgggcggca
     8041 tctggagatt cgcgtcatgc cttataccca caaacagttg ctgatggtgg cgcgagatgt
     8101 cacgcaaatg catcaactgg aaggggcgcg gcgtaacttt tttgccaacg tgagccatga
     8161 gttacgtacg ccattgaccg tgttacaggg ttacctggag atgatggatg agcaaccgct
     8221 ggaaggcgcg gtacgtgaaa aagcgttgca caccatgcgc gagcagaccc agcggatgga
     8281 agggttggtg aagcaattgc tgacgctgtc gaaaatagaa gccgcaccga cgcatttgct
     8341 caatgaaaag gttgatgtgc cgatgatgct gcgcgtcgtt gagcgcgagg ctcagactct
     8401 gagtcagaaa aaacagacat ttacctttga gatagataac ggcctcaagg tgtcaggcaa
     8461 tgaagatcag ctacgcagtg cgatttcgaa cctggtctat aacgccgtga atcatacgcc
     8521 ggaaggcacg catatcaccg tacgctggca gcgagtgccg cacggtgccg aatttagtgt
     8581 tgaagataac ggaccgggca ttgcaccgga gcatattccg cgcctgaccg agcgttttta
     8641 tcgcgttgat aaagcgcgtt cccggcaaac cggtggtagc ggattagggt tagcgatcgt
     8701 gaaacatgcg gtgaatcatc acgaaagtcg cctgaatatt gagagtacag taggaaaagg
     8761 tacacgtttc agttttgtta tcccggaacg tttaattgcc aaaaacagcg attaattcgc
     8821 ctttgtcatc ttttattgcc ataagccagc cgatgctggc ttattttctt tgcagtcgaa
     8881 atacgggcgt tagattttac aatggttggt gattttttgt tcgcatgatt agccatgtct
     8941 ttttcattga aatagtgttt tatactggtt ggtgatttct tatcgctata tacctctggt
     9001 ttttagatcc cttcttgctt taaaacgtta taagcgttta aattgcgctt caggtgctgt
     9061 catactgact gcattcacgc ggtaaatcga aaaactattc ttcgccgcgc ctggttggga
     9121 gtatttcccg ctaaaattgt ttaaatatac cgctgtatca tccccaggga ttggcacaaa
     9181 aatttaacgt tacaacacca catccacagg cagtatgatt tatgacccat caattaagat
     9241 cgcgcgatat catcgctctg ggctttatga catttgcgtt gttcgtcggc gcaggtaaca
     9301 ttattttccc tccaatggtc ggcttgcagg caggcgaaca cgtctggact gcggcattcg
     9361 gcttcctcat tactgccgtt ggcctaccgg tattaacggt agtggcgctg gcaaaagttg
     9421 gcggcggtgt tgacagtctc agcacgccaa ttggtaaagt cgctggcgta ctgctggcaa
     9481 cagtttgtta cctggcggtg gggccgcttt tcgctacgcc gcgtacagct accgtttctt
     9541 ttgaagtggg cattgcgccg ctgacgggtg attccgcgct gccgctgttt atttacagcc
     9601 tggtctattt cgctatcgtt attctggttt cgctctatcc gggcaagctg ctggataccg
     9661 tgggcaactt ccttgcgccg ctgaaaatta tcgcgctggt catcctgtct gttgccgcaa
     9721 ttatctggcc ggcgggttct atcagcacgg cgactgaggc ttatcaaaac gctgcgtttt
     9781 ctaacggctt cgtcaacggc tatctgacca tggatacgct gggcgcaatg gtgtttggta
     9841 tcgttattgt taacgcggcg cgttctcgtg gcgttaccga agcgcgtctg ctgacccgtt
     9901 ataccgtctg agctggcctg acggcgggtg ttggtctgac tctgctgtac ctggcgctgt
     9961 tccgtctggg ttcagacagc gcgtcgctgg tcgatcagtc tgcaaacggt gcggcgatcc
    10021 tgcatgctta cgttcagcat acctttggcg gcggcggtag cttcctgctg gcggcgttaa
    10081 tcttcatcgc ctgcctggtc acggcggttg gcctgacctg tgcttgtgca gaattcttcg
    10141 cccagtacgt accgctctct tatcgtacgc tggtgtttat cctcggcggc ttctcgatgg
    10201 tggtgtctaa cctcggcttg agccagctga ttcagatctc tgtaccggtg ctgacagcca
    10261 tttatccgcc gtgtatcgca ctggttgtat taagttttac ccgctcatgg tggcataatt
    10321 cgtcccgcgt gattgctccg ccgatgttta tcagcctgct ttttggtatt ctcgacggga
    10381 tcaaggcatc tgcattcagc gatatcttac cgtcctgggc gcagcgttta ccgctggccg
    10441 aacaaggtct ggcgtggtta atgccaacag tggtgatggt ggttctggcc attatctggg
    10501 atcgtgcggc aggtcgtcag gtgacctcca gcgctcacta aatcactgaa catttgtttt
    10561 aaccacgggg ctgcgatgcc ccgtggtttt ttattgtgtt gatgggttag gaattgatgg
    10621 aaagtaagaa caagctaaag cgtgggctaa gtacccgaca catacgcttt atggcactgg
    10681 gttcagcaat tggcaccggg ctgttttacg gttcggcaga tgccatcaaa atggccggtc
    10741 caagcgtgtt gttggcctat attatcggtg gtatcgcggc gtatatcatt atgcgtgcgc
    10801 tgggggaaat gtcggtacat aacccggcag ccagctcttt ctcgcgttat gcgcaggaaa
    10861 acctcggccc gctggcaggt tacattaccg gctggaccta ctgctttgaa atccttattg
    10921 tcgccatcgc cgatgtgacc gcttttggta tctatatggg tgtctggttc ccgacggtgc
    10981 cgcactggat ttgggtactg agtgtggtgc tgatcatttg cgccgtaaac ctgatgagcg
    11041 tgaaggtatt cggtgagctg gagttctggt tctcgttctt taaagtcgcc actatcatca
    11101 tcatgattgt cgccggtttc ggcatcatca tctgggggat tggcaacggc gggcaaccga
    11161 ccggtattca taacctgtgg agcaacggcg gcttcttcag taacggctgg cttggtatgg
    11221 tgatgtcgtt gcaaatggtg atgtttgctt acggtgggat cgaaattatc gggattaccg
    11281 ccggtgaagc gaaagatcct gagaaatcga ttccgcgtgc gattaactcc gtgccgatgc
    11341 gtattctggt gttctacgtc ggtacgctgt tcgtcattat gtctatctac ccgtggaatc
    11401 aggttggcac tgccggtagc ccgtttgtgc tgacgttcca gcatatgggc attacctttg
    11461 ccgccagcat tcttaacttt gttgtgctga ccgcttcgct gtcggcaatt aacagtgacg
    11521 tatttggtgt aggccgtatg ctccacggta tggcagagca gggcagcgcg ccaaaaattt
    11581 tcagcaaaac ctcgcgtcgc ggtattccgt gggttacggt gctggtgatg actaccgcgc
    11641 tgctgtttgc ggtgtatctg aactacatca tgccggaaaa cgtcttcctg gtgatcgctt
    11701 cgctggcaac cttcgccacg gtgtgggtgt ggattatgat cctgctgtcg caaatcgcct
    11761 tccgtcgccg tttgccgcca gaagaagtta aggcgctgaa atttaaagtg ccgggtgggg
    11821 tcgcaaccac catcggcggt ttgattttcc tgctctttat tatcgggttg attggttatc
    11881 accctgatac gcgtatctcg ctgtatgttg gtttcgcgtg gattgttgtg ctgttgattg
    11941 gctggatgtt taagcgccgc cacgatcgtc agctggctga aaaccagtaa tccttgcgtt
    12001 ttgtaagccg gttcaggagg aaatcctgat ccggctattc cgaaagttat ccgcccccgt
    12061 cctcctcccc caaatatcct tcagatgatg agtgatcctg cattaggcta tggcaaggtg
    12121 atcagatttt catcacaggg gaattatgat gttaaatgca tggcatctgc cggtgccccc
    12181 atttgttaaa caaagcaaag atcaactgct cattacactg tggctgacgg gcgaagaccc
    12241 accgcagcgc attatgctgc gtacagaaca cgataacgaa gaaatgtcag taccgatgca
    12301 taagcagcgc agtcagccgc agcctggcgt caccgcatgg cgggcggcga tcgatctctc
    12361 cagcggacaa cctcggcggc gttatagttt caaattgctg tggcacgatc gccagcgttg
    12421 gtttacaccg caaggcttca gccgaatgcc gccagcacga ctggagcagt ttgccattga
    12481 tgtaccggat atcggcccac aatgggctgc ggatcagatt ttttatcaga tcttccctga
    12541 tcgtttcgcc cgtaatcttc ctcgtgaagc tgaacaggat catgtctatt accatcacgc
    12601 agccggacaa gagatcatat tgcgtgactg ggatgaaccg gtcacggcgc aggctggcgg
    12661 atcaacgttc tatggcggcg atctggacgg gataagtgaa aaactgccgt atctgaaaaa
    12721 acttggcgtg acggcgctgt atctcaatcc ggtgtttaaa gctcccagcg tgcataaata
    12781 cgataccgag gattatcgcc atgtcgatcc gcagtttggc ggtgatgggg cgttgctgcg
    12841 tttgcgacac aatacgcagc agctgggaat gcgactggtg ctggacggcg tgtttaacca
    12901 cagtggcgat tcccatgcct ggtttgacag gcacaatcgt ggcacggggg gagcttgtca
    12961 caaccccgaa tcaccctggc gtgactggta ctcgtttagt gatgatggca cggcgctcga
    13021 ctggctgggc tatgccagct tgccgaagct ggattatcag tcggaaagtc tggtgaatga
    13081 aatttatcgc ggggaagaca gtattgtccg ccactggctg aaagcgccgt ggaatatgga
    13141 tggctggcgg ctggatgtgg tgcatatgct gggggaagcg ggtggggcgc gcaataattt
    13201 gcagcacgtt gccgggatca ccgaagcggc gaaagaaacc cagccggatg cgtatattgt
    13261 cggcgaacat tttggcgatg cacggcaatg gttacaggcc gatgtggaag atgccgccat
    13321 gaactatcgc ggcttcacat tcccgttgtg gggatttctt gccaacaccg atatctctta
    13381 cgatccgcag caaattgacg cccaaacctg tatggcctgg atggataatt accgcgcagg
    13441 gctttctcat caacaacaat tacgtatgtt taatcagctc gacagccacg atactgcgcg
    13501 atttaaaacg ctgctcggtc gggatgttgc gcgcctgccc ctggcggtgg tctggctgtt
    13561 cacctggcct ggtgtaccgt gcatttatta cggtgatgaa gtgggactgg atggcaaaaa
    13621 cgatccgttt tgccgtaagc cgttcccctg gcaggtggaa aagcaggatt cggcgttatt
    13681 cgcgctgtac cagcgaatga ttgcgctgcg taagaaaagt caggcgttgc gtcgtggcgg
    13741 ctgtcaggtg ctgtatgcgg aagataacgt ggtgttattt gtccgcgtgc tgaatcagca
    13801 gcgtgtactg gtggcaatca accgtggcga agcctgtgaa gtggtgctac ccgcgtcgcc
    13861 gttgcttaat gtcgcgcaat ggcaacgcaa agaaggtcac gggcaactga cggacgggat
    13921 tctggctttg cctgccattt cggctacggt gtggatgaat taacgtctta taacgccttg
    13981 cgtgacgcct gctccatcat ccgcggataa aactgccaga agcgggtttc gagagcatcg
    14041 taatgggcgt ctaaatcgta ccaggagtca cgcagggcat ccagacgtgg gcgacggctt
    14101 gccatgccgt ttaacacgct ctggatgaaa tccatatcgc gatagcgcac caaccactgc
    14161 tctgaccata agtagttgtt cagattgata aagcgtggcg gnnnnnnnnn ntaacaggta
    14221 aattcctgta gcggaaaatc gggcgacagc tgcgaccagt ggcgggacag aaagtgatcc
    14281 cacatcacat ccagcgtaat aggcgcgaca cggcgcgttt cattacgaaa ccactcccgc
    14341 gcttcgcgga cttctggcag attgtcggtt aatacgtcga ttcgccgatg catatgaatg
    14401 ccagccacga cgtcgggcgg aaaactttct tcaggatttc cgcgtacgaa atcagccagt
    14461 aaattgccgg aaagcgagct ttccgcgaga tgggctaaat gcaggtgagc taaaaaattc
    14521 atcgattata ttctatccaa aggggggtaa aggttgcagg gagagcgccc cggcactaga
    14581 ctacccgcct cttattttag tctgagtcag tgtcatgcgc gttaccgatt tctcttttga
    14641 attgcccgaa tccctgattg cccactatcc catgcctgaa cgcagtagct gtcgtttact
    14701 gtcgctggac gggccgacgg gcgcgctgac gcacggtact ttcaccgatt tactcgataa
    14761 gctcaacccc ggcgatcttc tggtttttaa taatacccgc gtgatcccgg cgcgtctgtt
    14821 tgggcgtaaa gccagcggcg gcaagattga agtgctggtt gagcggatgc tcgacgacaa
    14881 acgcattctt gcgcatattc gcgcctcgaa agcgccaaaa cctggcgcag aactgctgct
    14941 gggcgatgac gaaagtatta acgcaacaat gaccgcgcgc cacggcgcac tgtttgaagt
    15001 cgaatttaat gatcaacgtt cggtgctgga tattctcaac agcatcggcc atatgccgct
    15061 gccgccgtac atcgaccgtc cggacgaaga tgcagaccgc gaactttatc aaacggttta
    15121 tagcgaaaaa cccggcgcgg tagcagcacc gaccgccgga ctgcactttg acgaaccttt
    15181 gctggaaaaa ttacgcgcca aaggcgtgga gatggcgttt gtgacgttgc acgttggtgc
    15241 gggcaccttc cagccggtgc gcgtcgacac cattgaagat cacatcatgc actcggaata
    15301 cgctgaagtg ccgcaggatg tggtagacgc ggtactggcg gcgaaagcgc gcggtaaccg
    15361 ggtgattgcg gttggtacca cctcagtacg ttcgctggaa agtgcggctc aggcagcgaa
    15421 aaacgatctc attgaaccgt tctttgacga tacccaaatc tttatctatc cgggcttcca
    15481 gtacaaagtg gtcgatgcgc tggtgacgaa cttccacctt ccggaatcga cgctgattat
    15541 gctggtttcg gcatttgctg gttatcaaca caccatgaat gcctataaag cggcggtaga
    15601 agagaaatat cgctttttta gttacggtga tgcgatgttt atcacgtaca atccgcaggc
    15661 aattaatgag cgcgttgggg agtaattccg cggcgctggt ttaaacgttg gactgttttt
    15721 ctgacgtagt ggagaaaaaa tgaaatttga actggacacc accgacggtc gcgcacgccg
    15781 tggccgcctg gtctttgatc gtggcgtagt ggaaacgcct tgttttatgc ctgttggcac
    15841 ttacggcacc gtaaaaggga tgacgccgga agaagttgaa gccactggcg cgcaaattat
    15901 cctcggcaac accttccacc tgtggctgcg cccgggccag gaaatcatga aactgcacgg
    15961 cgatctgcac gattttatgc agtggaaagg accaatcctt accgactccg gcggcttcca
    16021 ggtcttcagc cttggtgata ttcgtaaaat caccgaacag ggcgttcact tccgtaaccc
    16081 gatcaacggc gacccgattt tcctcgaccc ggaaaagtcg atggagattc agtacgatct
    16141 tggttcggat atcgtcatga tctttgatga gtgtacgccg tatcctgctg actgggatta
    16201 cgcaaaacgc tccatggaga tgtctctgcg ttgggcgaag cgtagccgtg agcgttttga
    16261 cagtctcgga aacaaaaatg cgctgtttgg tatcattcag ggcagtgttt acgaagattt
    16321 acgtgatatt tctgttaaag gtctggtaga tatcggtttt gatggctacg ctgtcggcgg
    16381 tctggctgtg ggtgagccga aagcagatat gcaccgcatt ctggagcatg tatgcccgca
    16441 aattccggca gacaaaccgc gttacctgat gggcgttggt aaaccagaag acctggttga
    16501 gggcgtacgt cgcggtatcg atatgtttga ctgcgtaatg ccaacccgca acgcccgaaa
    16561 tggtcatttg ttcgtgaccg atggcgtggt gaaaatccgc aatgcgaagt ataagagcga
    16621 tactggccca ctcgatcctg agtgtgattg ctacacctgt cgcaattatt cacgcgctta
    16681 cttgcatcat ctcgaccgtt gcaacgaaat attaggcgcg cgactcaaca ccattcataa
    16741 ccttcgttac taccagcgtt tgatggcggg tttacgcaag gctattgaag agggtaaatt
    16801 agagagcttc gtaactgatt tttaccagcg tcaggggcga gaagtaccac ctttgaacgt
    16861 tgattaatat taataatgag ggaaatttaa tgagcttttt tatttctgat gcggtagcgg
    16921 caacgggtgc accggcgcaa ggtagcccga tgtctttgat tttgatgctg gtggtattcg
    16981 gtctgatttt ctatttcatg atcctgcgtc cacagcagaa gcgcaccaaa gaacacaaaa
    17041 agctgatgga ctccatcgct aaaggtgatg aagtgctgac gaacggtggc ctggtcggtc
    17101 gcgtaaccaa agtagcggaa aacggctaca ttgctatcgc actgaatgac accactgaag
    17161 tagttattaa acgtgacttc gtagctgccg ttctgccgaa aggcaccatg aaggcgctgt
    17221 aattaaaatt tttccctaag ggaattgccg tgttaaaccg ttatcctttg tggaagtacg
    17281 tcatgctgat cgtggtgatt gtcatcggtc tgctgtatgc gcttcccaac ctgtttggtg
    17341 aggatccggc tgttcagatc actggtgcgc gcggagtcgc cgccagtgag caaacgctga
    17401 tccaggtcca gaaaacgtta caagaagaaa aaataacttc taagtctgtg gcactggaag
    17461 agggcgctat tcttgcgcgc ttcgactcca ctgacaccca gctgcgcgct cgtgaagcat
    17521 taatgggcgt tatgggtgac aaatacgtcg tggcgcttaa tcttgccccg gcaacgccgc
    17581 gctggctggc agccattcat gctgagccga tgaagctcgg ccttgacctg cgtggcggcg
    17641 ttcacttcct gatggaagtg gatatggaca ccgcgcttgg caaactccag gaacaaaata
    17701 tcgatagcct gcgcagcgac ctgcgtgaaa agggcatccc gtataccacc gttcgtaaag
    17761 aaaacaacta cggcctgagc atcactttcc gcgatgctaa agctcgtgat gaagccattg
    17821 cgtatctgag caagcgccat ccggacctgg tgattagcag ccagggcagc aaccagctgc
    17881 gtgcggtaat gagcgatgct cgtctgagtg aagcgcgtga atatgcggtg cagcagaaca
    17941 tcaatatcct gcgtaaccgt gtaaaccaac ttggcgtggc ggagccggtg gttcagcgtc
    18001 agggtgctga ccgtatcgtt gttgaactgc caggtattca ggacactgcg cgtgcgaaag
    18061 agattctggg tgcgacggca acgctggaat tccgtctggt aaacaccaac gttgatcagg
    18121 ccgctgcggc atccggtcgc gtaccgggcg actctgaagt gaaacagacc cgcgaaggtc
    18181 agccggttgt gctgtacaaa cgcgtaattc tgaccggtga ccatatcacc gactccactt
    18241 ccagccagga cgaatacaac cagccgcagg ttaacatctc gctcgatagc gcgggtggta
    18301 acatcatgtc taacttcacc aaggacaaca tcggcaaacc gatggcaact ctgtttgtgg
    18361 agtacaaaga cagcggtaag aaagatgcaa acggtcgtgc ggttctggtg aaacaggaag
    18421 aggtgattaa catcgccaac atccagtctc gtctgggtaa cagcttccgt attactggca
    18481 tcaataaccc aagcgaagcg cgtcagcttt cactgctgct gcgtgccggt gcgttgatcg
    18541 cgccgattca gattgttgaa gaacgtacca ttggcccaac cctgggtatg cagaacattg
    18601 agcaggggct ggaggcttgc cttgccggtc tgctggtttc tattctgttc atgatcatct
    18661 tctataagaa gtttggtctg attgcgacca gtgctctgat tgccaacttg atcttgattg
    18721 tcggtattat gtcgctgttg ccaggcgcaa cgctgagtat gccaggtatc gcgggtatcg
    18781 tcttaaccct tgcggtagcg gtcgatgcga acgtactgat caacgaacgt attaaagaag
    18841 agttgagcaa cggacgtact gttcaacagg caattgatga aggttatcgt ggcgcattca
    18901 gttccatctt cgatgcgaac atcaccacgc tgattaaagt catcatcctg tatgcagtgg
    18961 gtaccggggc aattaaaggg ttcgcgatta ctaccggtat cggtgtggcg acgtcgatgt
    19021 ttaccgcgat tgtcggtacg cgtgccatcg taaacctgct atatggcggc aagcgcgtca
    19081 agaagctgtc aatctgagga gtgcgatgtg gcacaggaat atactgttga acaactaaac
    19141 cacggccgta aagtctatga ctttatgcgc tgggactact gggctttcgg catctctggt
    19201 ctgctgttaa tcgccgctat cgttattatg ggcgtgcgcg gctttaactg ggggctggat
    19261 tttaccggtg gtacggttat tgaaattacg ctcgaaaaac cggctgaaat tgacgtaatg
    19321 cgtgatgcat tgcaaaaagc cggttttgaa gagccgatgc tgcaaaactt tggtagcagc
    19381 cacgacatca tggtccgtat gccgcctgct gaaggcgaaa ccggcggtca ggtgctgggc
    19441 agccaggttc tgaaggtgat taacgaatcc accaatcaga atgcggcagt gaagcgtatt
    19501 gagttcgtcg gtccgagcgt gggggcagac cttgcgcaaa ccggtgcgat ggcgttgatg
    19561 gcggcgctgc tgtctatcct cgtgtacgta ggtttccgct ttgagtggcg actggcggca
    19621 ggggttgtta ttgcgctggc gcacgacgtt atcattacgc tgggtatttt gtcgttattc
    19681 catatcgaga ttgacctgac cattgtggca tcgttgatgt cggttatcgg ttactcgctt
    19741 aacgacagta tcgtggtatc ggaccgtatt cgtgaaaact tccgcaagat ccgtcgcggt
    19801 acgccttacg aaatctttaa cgtgtccttg acccagacgc tgcaccgtac cttgatcaca
    19861 tccggtacta ccttgatggt tatcctgatg ctgtacctct tcggtggtcc ggtactggaa
    19921 ggcttctcgc tgaccatgct tatcggtgtt tccatcggta ctgcatcttc catctatgtg
    19981 gcatctgcgc tggctctgaa actgggtatg aagcgcgaac acatgttgca gcagaaagtg
    20041 gaaaaagaag gggcggatca gccgtcaatt ctgccttaat caagtttcag ctgatgttga
    20101 aaatcccggt cagaagatcg ggattttttt tgatgtaagg tgattgcgaa aatccccgca
    20161 tcttgggaaa ctgcgcgtaa ccctacattt catccaggta actttttcat ggctatcatc
    20221 ccaaaaaact atgcgcggtt agaaagcggc tatcgcgaaa aagcattaaa aatctatccg
    20281 tgggtctgcg gtcgctgttc ccgcgagttt gtttattcca acctgcgtga acttaccgtt
    20341 caccacattg atcacgacca tactaataac ccggaagatg gcagtaactg ggaattactt
    20401 tgtctctatt gccacgatca tgagcattcg aaatacaccg aagcggatca gtatggtacg
    20461 accgttatcg caggggaaga tgcgcagaaa gatgtcggtg aagcgaagta caacccattc
    20521 gctgatttga aagcgatgat gaacaagaag aagtgataaa aaatgccgga tatgaaatcc
    20581 ggcattctca caattagaag ttgtaaccta ctaccagata accaccccag ccggtagagc
    20641 gaacgttgaa gttgccgttg ccgaagttca gttctgcatc gtcattccac tgaccaccgt
    20701 tatgccagta acgggcaacg acagagtagt gccagtgatc gtaattcagc gccagaatat
    20761 ggctggacgc aatagagtta ttggtacgtg ttttaatacc gttgttcgca taaccgctgt
    20821 catcgcccag gtcggaaccc cagtcgaagt tggtgaagcc gatgtagctc agctggccgc
    20881 cccacagatc ggtaatcggc acaaagtact tcactttgaa acggtaaccg tcccactcgt
    20941 tttcgttcgc tgcgccgtag ttctgccact ggtatttcgc atagacgttc atggacaggc
    21001 tcatcggcag accagtatcg atgtcggtac ccagacccat gtaccaggtg ctctggcggc
    21061 catctttatt acgacccatg tcgtaaatgt agttgttcgc gaagtaccac tctttgaacg
    21121 gaccaaagct aaggtcagta ttggtcagct tgtcgatgga gaaacgcggt tcgatttcca
    21181 taaacagcgg agaaccgtgg ttccagatac cttttgcatc ggagttaccg ccgaagaata
    21241 ccggcgcatc cgcataacca tagaagtcga accagtcttt tttagcgaat gcttcgtact
    21301 caaggtaggt atcgttgcgg agctgcggtc cgaaacgggt gtgatagctt ccgacaacgt
    21361 taacgctctg gtgccaccag tcggaaagat actgcggttt gtcgttttca gctgcgttga
    21421 cagtaaaaga cgaagagagc gccagtaccg caccggctgc cagtaatgtt tttttcatat
    21481 gtatgccact gtttgaaaat cccttgcggg agtgaaaaag gcgcaaattg cgtttctaaa
    21541 tatttcgtat acagaggagc ctattataaa aatcattgct cacaaaaata tgtttcgttt
    21601 cacagttcta tcatttacgt aatcgattgc gttcacgttt acacacattc gggcggggat
    21661 tgtactgact ttcacccttg ttgcaaactt ttactatcaa tccaggtgat gtaagcggag
    21721 taacaaaatg acggagaaag atgttgcggg acgcacaata tcgccccgca atacgtttac
    21781 tgcataacag gttgaatgtc gtggatacgt acaaagccta actgatccac agacataccc
    21841 tttaactgta acggaatatc aacatcgctg ggggcgagga cgctggcagg tgcgttaatc
    21901 aattgattct gcacattgat ttcctgaaag ttgtctattg ttccctgaat ttgcccatat
    21961 tcaacggttc cgctgaatgc gggtaaaggg tcattggatt cgccctgaat acgtagcgtc
    22021 agggtggtgc catctgtatc aggcgtaata ttcaccagcg acatacgtaa agtaccgatc
    22081 tggctttcca gtcttgctgg tgtttttgct ccaggcagaa ggtaaacccc actgctggat
    22141 tttgcattca gcctgttttg ctgagtgatt ttgaccgttt ctttattgag ctgcgtcatc
    22201 tcctggttca gcgtgctgac gctgtgtttc atctgtcgca cttcactttg ctgcacacag
    22261 gcgctaaggc tgaacaggct tcccagcagg agcagtcgaa aaacgttagt attcataggt
    22321 cgtccctttc tcgtcgcaat tagcctaatg gtagagggta aaagcagtga aagcatcgat
    22381 cctttatctc aaaagcgttg cgcctttgtt gtatcgtcag ttcaggttaa aatagatttc
    22441 tgttaaccac ctggtcagga cgccgtatgc attgcccatt ctgtttcgcc gtggacacta
    22501 aggtaattga ctctcgtctc gtgggcgagg gttcatccgt acgccgccgt cggcagtgtc
    22561 tggtgtgtaa tgaacgtttc accacctttg aagtggcgga gctggttatg ccgcgtgttg
    22621 taaaaagcaa cgacgtgcgt gaaccgttta atgaagagaa attacgtagc ggaatgctgc
    22681 gggcgctgga aaaacgtccg gtgagttccg atgacgtcga aatggcaatc aatcatatta
    22741 aatcgcagct gcgcgccacc ggtgagcgcg aagtgccgag caagatgatt ggcaatctgg
    22801 tgatggagca attgaaaaag ctcgataaag tcgcctatat ccgttttgcc tctgtctacc
    22861 gcagtttcga agatatcaaa gaatttggcg aagagatcgc gcgcctggag gactaagccg
    22921 tgcaggacga gtattacatg gcgcgggcgc taaagctggc gcaacgagga cgttttacca
    22981 cgcatcccaa cccgaatgtc gggtgcgtca ttgtcaaaga tggcgaaatt gtcggtgaag
    23041 gttaccatca ccgtgctggt gaaccacacg ccgaagtaca tgcgttgcgt atggctggag
    23101 aaaaagccaa aggtgcgact gcgtatgtga cactcgaacc ttgcagccat cacggccgta
    23161 cgccgccttg ctgtgatgca ttaattgccg caggtgtggc gcgcgtggtt gcctcgatgc
    23221 aagatcctaa cccgcaggta gctgggcgag ggctatatcg tctgcaacag gctggcattg
    23281 acgtcagcca cgggctgatg atgagtgaag ccgagcaatt gaataaaggc tttctcaaac
    23341 ggatgcgcac cggttttcct tatattcaat taaaacttgg cgcatcgctt gatggtcgca
    23401 cggcgatggc gagtggcgaa agccagtgga tcacttcgcc tcaggcgcga ctcgatgtac
    23461 aacgactgcg cgcacaaagt catgccattt taaccagcag cgccacggtg ctggcggatg
    23521 atccagcctt aacagtgcgt tggtctgaac tggatgaaca aactcaggcg ctttatccgc
    23581 aacaaaatct ccgtcagccg atacgtattg tgattgatag ccaaaatcgc gtgacgccgg
    23641 aacatcgcat tgtgcagcag cccggcgaaa cttggttcgc gcgtacgcag gaagattctc
    23701 gtgagtggcc ggaaacggtg cgtaccttgc tgattccaga gcataaaggt catctggatc
    23761 tggttgtact gatgatgcaa ctgggtaaac agcaaattaa cagcatctgg gtggaagcgg
    23821 ggccaacgct cgctggcgca ttgctacagg ctggtttagt tgatgagctg attgtctata
    23881 tcgcacctaa actattaggc aacgacgccc gtggattatg cacgctgcca gggcttgaga
    23941 aattagccga cgctccccaa tttaaattca aagagatacg tcatgtaggc ccggatgttt
    24001 gcctgcattt agtgggtgca tgatctcccg gctcgaaagg gaagcagcgc acgaaatatt
    24061 atgctaaaat ccgcccccct gcggggccat actcgaaccc gaaggaagaa aatgaacatt
    24121 attgaagcta acgttgctac cccggacgct cgcgtcgcca tcaccatcgc gcgtttcaac
    24181 aactttatca atgacagcct gctggaaggt gcaattgacg cactgaaacg tatcggtcag
    24241 gtaaaagatg aaaacattac cgttgtttgg gtgcctggtg cctatgagct gccgctggcg
    24301 gcgggtgcac tggctaaaac cggtaaatac gacgcggtga ttgcgctggg tacggttatt
    24361 cgtggtggca ctgcccactt tgaatatgtc gctggtggtg caagcaacgg cctggcgcat
    24421 gttgcccagg acagcgaaat tccggttgct tttggggttc tgaccactga aagcattgaa
    24481 caagcgatcg aacgtgctgg caccaaagct ggcaacaaag gtgcagaagc tgcactgacc
    24541 gcgcttgaaa tgattaatgt attgaaagcc atcaaggcct gaaattagta aggggaaatc
    24601 cgtgaaacct gctgctcgtc gccgcgctcg tgagtgtgcc gtccaggcgc tctactcctg
    24661 gcagttgtcc cagaacgaca tcgctgatgt tgaataccag ttcctggctg aacaggatgt
    24721 aaaagacgtt gacgtcctgt acttccgtga gctgctggcc ggggtggcga ctaacaccgc
    24781 atacctcgac ggactgatga agccgtacct gtcacgtctg ctggaagaac tgggccaggt
    24841 agaaaaagca gtactgcgca ttgcgctgta cgagctgtct aaacgtagcg atgtgccata
    24901 caaagtggcc attaacgaag cgatcgaact ggcgaaatcg ttcggcgcag aagacagcca
    24961 taagttcgtc aacggcgtac tcgataaagc agcacctgtg attcgcccta acaaaaagta
    25021 atatccaggc cggtagattc acggaagacc gttccatgat cgccggcctt ttctttttta
    25081 cctgctgagg cataacgtat ggcatgtggc gagttctccc tgattgcccg ttattttgac
    25141 cgtgtaagaa gttctcgtct tgatgtcgaa ctgggcatcg gcgacgattg cgctcttctc
    25201 aatatccccg agaaacagac cctggcgatc agcactgata cgctggtggc gggcaaccac
    25261 ttcctccctg atatcgatcc tgctgatctg gcttataaag cactggcggt gaacctaagc
    25321 gatctggcag cgatgggggc cgatccggcc tggctgacgc tggcattaac cttaccggac
    25381 gtagacgaag cgtggcttga gtccttcagc gacagcttgt ttgatcttct caattattac
    25441 gatatgcaac tcattggcgg cgataccacg cgtgggccat tatcaatgac gttgggtatc
    25501 cacggctttg ttccgatggg acgagcctta acgcgctctg gggcgaaacc gggtgactgg
    25561 atctatgtga ccggtacacc gggcgatagc gctgccgggc tggcgatttt gcaaaaccgt
    25621 ttgcaggttg ccgatgctaa agatgcggac tacttgatca aacgtcatct ccgtccgtcg
    25681 ccgcgtattt tacaggggca ggcactgcgc gatctggcaa attcagctat cgatctctct
    25741 gacggtctga tttccgatct cgggcatatc gtgaaagcca gcgactgcgg cgcacgtatt
    25801 gacctggcat tgctgccgtt ttctgatgcg ctttctcgcc atgttgaacc ggaacaggcg
    25861 ctgcgctggg cgctctctgg cggtgaagat tacgagttgt gtttcacggt gccggaactg
    25921 aaccgtggcg cgctggatgt ggctctcgga cacctgggcg taccgtttac ctgtatcggg
    25981 caaatgaccg ccgatatcga agggctttgt tttattcgtg acggcgaacc tgtcacgtta
    26041 gactggaaag gatatgacca ttttgccacg ccataaagat gtcgcgaaaa gtcgcctgaa
    26101 aatgagtaat ccgtggcatc tgcttgctgt cggattcgga agtggattaa gcccgatcgt
    26161 tcctggcacg atggggtcgc tggcggcgat tccgttctgg tatctgatga cctttttgcc
    26221 ctggcagctc tattcgctgg tggtgatgct ggggatctgt atcggcgtct atctttgtca
    26281 tcaaacggcg aaagacatgg gcgtgcacga tcacggcagc attgtctggg acgaatttat
    26341 tggtatgtgg atcacgctca tggcgctgcc gaccaatgac tggcagtggg tcgcagccgg
    26401 gtttgtgatt ttccgtatcc tcgatatgtg gaaaccgtgg ccgatccgct ggtttgatcg
    26461 caatgtgcat ggcggcatgg ggatcatgat cgacgatatt gtcgccgggg tgatttccgc
    26521 aggcatcctg tactttatcg gtcatcactg gccgctgggt attctgtcgt agttgtgcac
    26581 cgatgcctga tgtgacgctt gtcacgtctc atcaggcctg catacctatt taaatcctac
    26641 gaccggatgc ggtttatacg gcgtttccag ctcggcgatc tgttccggct tcaaggtgat
    26701 atccaccgcg tttagcagtt catcaagctg ttcctcccga gaagttccga taatcggtgc
    26761 ggcaatgccc ggtttactca acaaccaggc cagcgcaact tgtgctcgcg ttgcaccaag
    26821 ttcttcactg acgcccgtta agcgttctgc aatttgtgcg tcattttcat tgctttcttg
    26881 gtagagattt ttccccactt catccgacac cagccgtgcg gtggtttctc cccaaggacg
    26941 cgtcagacgg ccccgcgcca gcgggctcca tggaatcacc gccacgcctt cctgataaca
    27001 cagcggcagc atctcgcgct cttcttcacg ataaatcaga ttgtagtgat cctgcatact
    27061 gacaaactgc gcccagccgt gctgtttttg tagttccagt gcttgagcaa actgcgaagc
    27121 gtgcatggat gacgcgccga tataacgcgc tttcccggct tttaccacgt cgttcagggc
    27181 ttccagcgtc tcttcgatcg gcgtgttgta atcccagcga tgaatttgca ggatatcgac
    27241 ataatccatg ccgagacggc gcaggctgtc gtcgatagag cgcaaaattt gtgcacggga
    27301 taatccttcc ggtaaatcac caacgcgatg gaacactttg gtcgcaacga ccacgtcttc
    27361 acgacgggcg aaatcccgca gtgcgcgacc gacgatctct tcgctgctgc catcggaata
    27421 gctattggcg gtgtcaaaga aatttatgcc gccttccagc gcacgtttaa tgatgggacg
    27481 gctgctctct tccggcagtg tccatgcgtg attaccgcga tctggctcgc caaaggtcat
    27541 acagccgagg caaagtcggg aaacacgaag gtcggttttt cctaaggggg tgtattgcat
    27601 gctgccactc ctgccatgaa taatttctta agcatagcag gagtagagta gggattatgc
    27661 cagccaggct ttgattttgg cttccatacc ggtggcatcg aggctgagtt cggcgcgcat
    27721 ttcttcctga gttccttgtg gaataaagaa gtccggcaga ccaatgttca gcacgggtac
    27781 nnnnnnnnnn tacgatgggc catcagcact tcgttcacgc cgctgcctgc accacccata
    27841 atggcgtttt cttctacggt aaccagcgct tcatggctgg cggccatttc cagaattaac
    27901 gcttcatcaa gcggtttcac gaaacgcata tcgaccagcg tagcgttcag cgattcagcg
    27961 actttcgccg cttctggcat cagcgtacca aagttaagga tcgccagttt ctcaccacga
    28021 cgcttcacaa tgcctttgcc aattggcagt ttttccagtg gcgtcagttc cacgccaact
    28081 gcgttgccgc gcgggtagcg caccgcggac gggccatcgt tatagtgata gccggtatag
    28141 agcatctggc gacattcgtt ttcatcgctc ggggtcataa tgaccatttc cggtatgcag
    28201 cgcaggtaag agagatcaaa agcgccctgg tgggtttgac cgtcagcacc aacaatgccc
    28261 gcacggtcga tggcgaacag gaccggaagc ttttgaatcg ccacgtcatg cagcacctga
    28321 tcataggcgc gttgcaggaa ggtggagtag atcgcaacaa tgggtttgta gcctccaatc
    28381 gccagacccg cagcaaaggt cactgcgtgt tgctcggcaa ttgccacgtc gaagtagcga
    28441 tccgggaatt tacgtgaaaa ctcgaccatg ccggaacctt cacgcatcgc cggagtaatc
    28501 gccatcagct tgttatcttt cgctgcggtt tcgcacaacc agtcgccaaa gatttttgaa
    28561 tagcttggca aaccgccgct acttttcggc agacaaccgc tggagggatc aaatttaggc
    28621 acggcgtgga aggtgattgg gtctttttct gccggttcat aaccacgacc ttttttggtc
    28681 atgatatgca ggaactgcgg gcctttcagg tcgcgcatgt tcttaagcgt ggtgataagc
    28741 cccagcacat cgtgaccgtc caccgggccg atgtagttaa agcccagctc ttcaaacaac
    28801 gtgccaggca ctaccatgcc tttaatatgt tcttcggtac gtttgagcag ctctttaatg
    28861 ggtggcacgc cagagaaaac tttttttccg ccttcgcgca gtgaagagta aagcttaccg
    28921 gaaagcagct gcgccagatg gttgttgagc gcgccaacat tttcggaaat cgacatttca
    28981 ttgtcgttga gaaccaccag catatcagga cggatatcgc ccgcgtgatt catcgcttca
    29041 aacgccatgc cagcagtaat cgcgccatcg ccaatgacac agac
//
LOCUS       DAWDRC010000002        28959 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_865_length_28959_cov_2.567084, whole genome shotgun sequence.
ACCESSION   DAWDRC010000002 DAWDRC010000000
VERSION     DAWDRC010000002.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28959)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 28959)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..28959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_865_length_28959_cov_2.567084"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6488..6497
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7838..7847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12404..12413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13943..13952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20697..20706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24098..24107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6953 a         7316 c         7528 g         7102 t
ORIGIN      
        1 tttcagtccc ctgctctacc gactgagcta tccgggcaac ggggcgcatt aaaccgtaat
       61 ctgcacatct cgtcaaccta atttcagtaa aagcgattca actgcttaag attgcggcaa
      121 atcgcttctt ttctgtgttt ctgtcaggtc agtgcaccac cctgacggca gcgggcgaag
      181 cgcaggatat cttccgccag ccgatgtgcg gtgtcgacat ccgcctggct acgattcacc
      241 agcattcggc ttaaacagcc ttccagcacc agttccatct gctttgctac catcgccgga
      301 tcgtcaactt ccagcgtggt taacagttcg tgggtgaaat cgtaggccgc gcttttttgc
      361 tgatcggcca gttgatgaat agggtggcca ggatcggggt aaaacgtaca ggcagcgata
      421 aacagacagc ccggatagcg gttgttttta acgcactccg ataacgcctg ataacgtgcc
      481 agcaactttt gttcggcggt ttgcgtttcg tccagcatca gctgacgacg ccagacatct
      541 atctgttggc taagataacg cagcgcatcg tagaggattg cctctttgtc tggccagaag
      601 cggcgtagct cgtccagtgg ataatccaca cgttcagcaa ccatctccag cgtggtgttg
      661 gcaatccctt gtaattctaa taatttcagg gcttctccca gtacatcttc acgttgcacg
      721 ctattttcct ccgtctttcc cactgcaagt gtcgttcacg gttggcgatc gcgcaaatgt
      781 gcgctgaagg tttcagcatc cataaagccc gtgacgcgtg cttgtggatg ctcctggcct
      841 tgtccgtcaa aaaagagaat tgtcggtagg ccaaggacat taagatgctt taacagcgcc
      901 acatcttgtg cgtcgttggc agtgacgttg gcctgaagta agaccgtatc tgctaacgct
      961 ttttgcacct gcgggtcgct gaaggtgtat ttctcaaact ctttacaggc gacgcaccag
     1021 tcggcataga gatctaacat cactggtttg cctttggctt caacgagcgc ctgatttaac
     1081 tcatctactg ttttgatttg tgtaaagttg agatgcgtct gagtttgcgc ggtatgtgtt
     1141 tcaccaaatg cccaatcctg aagtgggcgc acgctaacca atgccgctgc cagcaggatt
     1201 atttgcacca cgcgcatcca gccgcgtttg gcctgtaggc tggtgataaa ggcccagcca
     1261 aagaatgcga caccaagcgc cgaccacaag cgtaatcccc atatatcacc aatcactcgc
     1321 tccagcagga agaccggcag tgcgaggatc acaaaaccaa acgcggtttt gacttgttcc
     1381 atccacgggc cgcttttcgg cagcaagcgg ttaccaaaga cggtaattag catcagcggc
     1441 aggcccatgc ccagcgcata aagataaagc gtgccgccgc ccagccacat gttcccgctt
     1501 tgggcgatat acagcagaat cgcgctaagc ggtgcagtgg tgcatggtga acagatcagt
     1561 ccggcaatcg cccccataat aaacacaccg ccaggtgagc cgccctgttg gcgattgctc
     1621 atcagcgtga gacgtgtttg cagcgaagag gggagttgca gagtaaacaa gccaaacatt
     1681 gacatcgcca gcaaggtaaa gacgatggcg aggccaatga gcacgtatgg gtgctgtagc
     1741 gccgcctgga actgtaaccc tgcggcggca accaccagac ccagcgccgt gtaagtcagc
     1801 gccatcccct gcacataaat aaaggtcagc aacaatgctc tggcagtgga aagccgctgt
     1861 ttaccgccca gcacgatgcc agaaatcagt gggtacattg gcagcacgca tggcgtaaag
     1921 gcgataccaa taccgatcaa caacgcccag agcgcggaaa agggcaattg cgcggtgggc
     1981 tgctcttgct gcggaacaga cacaggctgt gacgcttcgt tgttagcgac cacttcgctt
     2041 aacggaacgg ttttggtttc tggcggataa cagaaaccgg catcagcaca gccctggtag
     2101 gtgacagtta acgttgctcc cgcactcgcc tggttgatgg ttacgggaag cgtcagccga
     2161 tcgcggtaaa tctcgctttt gccgtaaaac tcatcttcat gccagacgcc ttgcggcagc
     2221 tgcacgtcag caattttcgc gtgttccggc gtaatgcgga tctgtttacg gtagaggtag
     2281 taaccgtctt tgatctgcca ggtcagattc agatcatgtt gattttgctg aaaatcaaaa
     2341 gcaaaggctt gatccgcggg gacaaattgt gaacgtcccg gcgcgtcgaa taatccggca
     2401 aaaacggaag tgctgcaaag tagcaggatc agcgtaaaga tgcgttgagc catgagaggt
     2461 aatctgtgtc tccgtgtgta acaggtaaaa ccagaagttc cggggtttga tatggatgat
     2521 gagacttcag gcattccagc agtgcctgct ggtgagatac ggtggttttt aaaatcatct
     2581 gcacttcgta ctcttgctcc agcttacctt cccagtaata gagagaggta gcgccgggga
     2641 tcaaggtcgc gcaggccgcc agtttttccg ccagcacttt ggcggctaaa tcctgggctg
     2701 tcgcttcatc cggtgcggta catagcacca cgacagacgc ggtattcgaa cttttttcat
     2761 caagcataaa cacctcgcaa gaacagatgg aaccgcaaga gagaggtcac tatacaacgg
     2821 acgggggaag gatgttagtc ggcgggaaag aaagcggggc gtgaacgccc cgcgatgggt
     2881 cattacagca tgaagctacc cagcacgaag ccgaagcaaa cggccagggc aacacccaga
     2941 gtacccggga tgaagaacgg atggttgaag acgaatttac cgatacgggt agtacccgtg
     3001 tcatccatct gtaccgcagc aaccagcgtc gggtaggtcg gcagaatgaa cagaccagac
     3061 accgcagcga aagaagcaac agcggtcagc ggagaaacgt tcagtgccag agccatcggc
     3121 atcagtgctt ttgcggttgc agcctgggag tacagcagag cagaagcaaa gaagaggatg
     3181 acggccagca gccacggatg accctgaatc acttcaccag cggtatcttt gatccagtcg
     3241 atgttgttgg aaacgaaagt atcgcccagc cacgcaacac ccaggataca aatacaggcg
     3301 ctcatacctg ctttgaaggt gctggagttg agaatgttgt cggtatcgac tttacagata
     3361 acggtggtca gagttgcaac gctgagcatg atgatcagga ttgcgttggt ggtgttcatc
     3421 agtggttttt caaccagacc catgcttggg ctgttgatga ttgcatagat aaccacgcca
     3481 actacgccca gcaggaacag ccagacggac gtttttgcac cggatttgat ttcaatctgc
     3541 ttttcaccgc gcagttcaac caggccctct tccagacgct tgcgataaat cggatcgtca
     3601 gagagtttgg agttgaacag catagtgacc aggaaggaca tcaccagaac cgccagcagg
     3661 gtggacggga tgaccacgga gagcagatgg aggtagctga tgccatgacc ttccatcacg
     3721 gaagacatgt aaaccactgc cgctgagatt ggcgatgcgg tgatcgcaat ctgcgcggat
     3781 accactgcag tagacagcgg acggcaaggt ttaacgcctt gttccttcgc aacttcagcg
     3841 ataactggca gtgtcgccag agagatgttg ccagtaccag caaagatagt caggaaatag
     3901 gtcacgatcg gcgcgaggat cgtgatgtat ttcgggttac ggcgcagcag cttttctgtc
     3961 tgatgaacca gatagtccag accgccagca acctgcatgg cagaaatagc ggcgataacc
     4021 gccatgataa tggaaatgac atcgaacggg atgttaccgg gtttaacgcc aatagcggca
     4081 agaaccagca cccccaatcc gcctgcaaaa ccaataccta ttccccccaa tctggcgccc
     4141 aagaagatcg ccagcaaaac tatgatgagt tctacaacta gcatattagc cttccttgtt
     4201 ttttaacaag ttgatattaa attgttattt ttaagttact gctcacaaga aaaaaggcac
     4261 gccatctgac gtgccttctt tatttgtact accctgtacg attactgttc gctttcatca
     4321 gtatagcgtt ttgctttgta agccgggtgc atcagattct gtacggagaa aatatcgtca
     4381 agttccgctt cagtcaacag accgcgttcc agaacgactt cacgtacact cttaccggtt
     4441 tcggcacaga ttttacccac gatgtcaccg ttgtggtgac cgatgaacgg gttcaggtaa
     4501 gtaacgatac cgatagagtt gtaaacgtaa ccttcgcaca cttctttgtt agcagtgatg
     4561 ccgttaatgc atttttccag caggttgtag caagcgttgg tcagaatgtg aacggattcg
     4621 aacatggcct ggccaatgac tggctccata acgttcaact gcagctgacc tgcttctgct
     4681 gccatggtaa cagtggtgtc gttaccgatg actttgaagc atacctggtt aaccacttcc
     4741 ggaacaaccg ggtttacttt agctggcatg atggaagagc ctgcctgcag ttccggcagg
     4801 ttgatctcgt tcaggccggc acgtggacca gaagagagca agcgcaggtc gttacagatt
     4861 ttggacatct tcacagccag gcgtttcagc gcgccgtgaa ccataacata agcgccgcag
     4921 tcggaggtcg cttcgatcag gtcttcagcc ggtacgcatg ggaagccagt gacttcagcc
     4981 agttttttca ctgccagcgg agagtactct ttcggcgtgt tcagaccagt accgattgct
     5041 gttgcgccaa ggttaacttc cagcagcagt tcagcggtac gttggatgtt tttcacttct
     5101 tctttcagca ggatgctgaa ggcgcggaat tcctgaccga gggtcatcgg tactgcgtcc
     5161 tgcagctggg tacgacccat tttcaggatg tcctggaatt cgacagcttt acgttcaaag
     5221 ccttcacgca gttggttaat cgcatctacc agtttaatca gggaagagta aactgcgata
     5281 cggaaaccgg tcgggtaggc gtcgttagtg gactgacatt tgttaacatg gtcgttcggg
     5341 ttcaggtact gatattcacc tttctggtga cccatcagtt ccagaccgat attggccagc
     5401 acttcgttgg tgttcatgtt tacggaagta cctgcgccgc cctggtagac gtctaccggg
     5461 aactgatcca tgcattttcc gttgttcagg acttcatcac atgcggcaat gatggcattc
     5521 gctacacttt taggaatggt ttgcagctct ttgtttgcca tagctgcggc ttttttaacc
     5581 attaccatac cgcgaacaaa ttcaggaata tcactgattt tgttgttgct gatatagaag
     5641 ttttcaatcg ctctcagagt gtgaacacca tagtaggcat cagctggaac ttccctggta
     5701 cccaacagat cttcttcgat acgaatgttg tttgacatgt gaaccttctt tttcaagctg
     5761 ccaatgattt gctttaaaca cacagaatat atgtggtttc gaatgttttt cgaccgacga
     5821 ttatcccctg catcgaccga atacccgaga tcatatgctg cttgaggatt tctaccgtaa
     5881 cctggatcac tttaagtgtc ggtttttacc ccttaattat taatttgtga aatggatcac
     5941 cgctttggga ttactaccaa aaatagttgc gcaaacatct tgaaattttg ctaatgacca
     6001 caatataagc taaacgcgat tcgcaaccca ttcaggtagc cgggtttaac cggctgctat
     6061 tacaggagaa acctttgcgc tggttacctt ttattgccat tttcctttat gtctatattg
     6121 agatttcaat ctttattcag gttgctcatg tattgggggt attgctgacc ctcgtgctgg
     6181 ttatattcac gtcagttatc ggtatgtcac tggtacgtaa ccagggcttt aagaatttcg
     6241 tgctgatgca gcaaaaaatg gcggcgggtg aaaacccagc ggcggagatg attaaaagtg
     6301 tttcgctgat cattgccggt ttgctgcttt tattaccggg ctttttcacc gacttcctcg
     6361 gtcttctgct tttattgccg ccggtgcaaa agcatctgac agtgaagttg atgccgcatt
     6421 tgcgcttttc tcgcatgcca ggcggcggtt ttagcgccgg gaccggtggc ggtaatactt
     6481 ttgatggnnn nnnnnnnttt gatggtgagt accagcgaaa ggatgatgac cgcgaccgcc
     6541 ttgatcataa agacgatcgc caggattaat gtcgaaacgc cggattatgt ggttatgcca
     6601 tttcccggcg tttttcgttt tggcagaaac agccataacc ccgccagcat gatcagcgcg
     6661 tagagacttt tccagccgac cattgccagt aacagaacgc ataatagccc gccaaccact
     6721 gccagtagtc gataacgtcc ttgcaataat ttacagcctg ccagcatgca taacagataa
     6781 atcataataa agatgccatt ggcataaata ataagagcgt ccagattgat ctctaaagca
     6841 tgaatcacca acgtgctcac cacgcagcag ccgagcaccg cattgagggc attattcgga
     6901 atatggcgag aagagaggcg tgccaggtag tggtcaggat tatgttgcgc ctgcgaccag
     6961 accaggcggg cgaagctctg tatataaatg ttgagactgg caaagcaggc cagatagcca
     7021 attacgcagg caatccataa cgctcctaca ccgaacaact gcactacaat tttgggaagc
     7081 gatgctgccg ccgccatttg ttcaccatag gcgtcgaagt gtaagacgac taccgtacag
     7141 ccccaataga ctaatcctgc cagcagcagg ccaatcatca aagcacgagg aaaatcacgc
     7201 tctggatttt taaattccga ggcaagatgg gcaaatgctt ccagaccgac aaaacaccag
     7261 aacatcactg ataacgcagc aaataacccg gtaagttcga tatttcctgg cacagggaag
     7321 gggatattcg caggtttgat atcgcccgcc caccagatag cgacaatcag tgcgacgata
     7381 agtccggcaa taactgtttg tagattagca ctggaactgg caccgcgagt accgatatac
     7441 cacaccagcg ccagcgtacc gagttctgcc aacaacagtt gctcgctatg ccagccaaac
     7501 attgcctggc cgaatccggc agcaatttgt agcgcggcag gcaaacccac gggaatgacc
     7561 gataaaaaca accagccggt gactcgctca agccgcgaac caaacgccat accgacgaag
     7621 tgtgcgacgc cgcctgcgct gggatagtgg cgacccagaa tcgcaaacac aatcgcaatc
     7681 gggaacacta agataatcaa aacgggccac gcccacaggc tgttattgcc tgctactagc
     7741 gcagctaacg caggaacggc aaacacgcca gtgcctaata atgacgtcga tagcaggccg
     7801 atgccctggg ccagccccag ttcttgtttg agtccacnnn nnnnnnngtc cactcatggg
     7861 ttgatgtccg attgcgccca aattttgggc aactgcgtag attttcgatg gtagcacaat
     7921 cagattcgct tatgacggtg atgaagaaat agcgatgaaa tgtgaggtga atcagggttt
     7981 tcacccgatt ttgtgctgat cagaattttt tttctttttc ccccttgaag gggcgaagcc
     8041 tcatccccat ttctctggtc accagccggg aaaccacgta agctccggcg tcacccataa
     8101 tagatacgga cttccataat agatacggac tttctcaaag gagagttatc aatgaatatt
     8161 cgtccattgc atgatcgcgt gatcgtcaag cgtaaagaag ttgaaactaa atctgctggc
     8221 ggcatcgttc tgaccggctc tgcagcggct aaatccaccc gcggcgaagt gctggctgtc
     8281 ggcaatggcc gtatccttga aaatggcgaa gtgaagccgc tggatgtgaa agttggcgac
     8341 atcgttattt tcaacgatgg ctacggtgtg aaatctgaga agatcgacaa tgaagaagtg
     8401 ttgatcatgt ccgaaagcga cattctggca attgttgaag cgtaatccgc gcacgacact
     8461 gaacatacga atttaaggaa taaagataat ggcagctaaa gacgtaaaat tcggtaacga
     8521 cgctcgtgtg aaaatgctgc gcggcgtaaa cgtactggca gatgcagtga aagttaccct
     8581 cggtccgaaa ggccgtaacg tagttctgga taaatctttc ggtgcaccga ccatcaccaa
     8641 agatggtgtt tccgttgctc gtgaaatcga actggaagac aagttcgaaa atatgggtgc
     8701 gcagatggtg aaagaagttg cctctaaagc gaacgacgct gcaggcgacg gtaccaccac
     8761 tgcaaccgta ctggctcagg ctatcatcac tgagggtctg aaagctgttg ctgcgggcat
     8821 gaacccgatg gacctgaaac gtggtatcga caaagcagtt accgctgcag ttgaagaact
     8881 gaaagcgctg tccgtaccgt gctctgactc taaagcgatt gctcaggttg gtaccatctc
     8941 tgctaactcc gacgaaaccg taggtaaact gatcgctgaa gcgatggaca aagtcggtaa
     9001 agaaggcgtt atcaccgttg aagacggtac cggtctgcag gacgaactgg acgtggttga
     9061 aggtatgcag ttcgaccgtg gctacttgtc tccttacttc atcaacaagc cggaaactgg
     9121 cgcagtagaa ctggaaagcc cgttcatcct gttggctgac aagaaaatct ccaacatccg
     9181 cgaaatgctg ccggttctgg aagctgttgc caaagcaggt aaaccgctgc tgatcatcgc
     9241 tgaagatgtt gaaggcgaag cactggcaac tctggttgtt aacaccatgc gtggcatcgt
     9301 gaaagttgct gcggttaaag ctccgggctt cggcgatcgt cgtaaagcta tgctgcagga
     9361 tatcgcaacc ctgactggcg gtaccgtaat ctctgaagag atcggtatgg agctggaaaa
     9421 agcaaccctg gaagacctgg gtcaggctaa acgtgttgtg atcaacaaag acaccaccac
     9481 catcatcgat ggcgtgggtg aagaagctgc aatccagggc cgtgttgctc agatccgtca
     9541 gcagattgaa gaagcaactt ctgactacga ccgtgaaaaa ctgcaggagc gcgtggcgaa
     9601 actggcaggc ggcgttgcag ttatcaaagt tggtgctgct accgaagttg aaatgaaaga
     9661 gaaaaaagca cgcgttgaag acgccctgca cgcgacccgt gctgcggtag aagaaggcgt
     9721 ggttgctggt ggtggtgttg cgctgatccg cgtagcgtct aaactggctg acctgcgtgg
     9781 tcagaacgaa gaccagaacg tgggtatcaa agttgcactg cgtgcaatgg aagctccgct
     9841 gcgtcagatc gtcctgaact gcggcgaaga accgtctgtt gttgctaaca ccgttaaagg
     9901 cggcgacggc aactacggtt acaacgcagc aactgaagaa tacggcaaca tgatcgatat
     9961 gggtatcctg gatccaacca aagtaacccg ttctgctctg cagtacgcgg cttctgtggc
    10021 tggcctgatg atcaccaccg agtgcatggt taccgacctg ccgaaaaacg atgcagctga
    10081 cttaggcgct gctggcggta tgggcggcat gggtggcatg ggcggcatga tgtaattgcc
    10141 ctgcacctcg cagaaataaa caaacccccg ggcagaaatg tctgggggtt tttcttttgg
    10201 tcatctttct agtataagat tcagacacgg acgacgcgag tggcgtccag ctcattgatt
    10261 atggggaata acatgcacgt aaaatactta gcagggattg tcggtgccgc gctactgatg
    10321 gcgggttgta gctccagcaa cgaattgagt gctgccggtc agagtgtacg cattgtggac
    10381 gagcaaccag gtgcagagtg ccagctgatt ggtactgcga caggtaagca aagcaactgg
    10441 ctttccgggc aacacggaga agaaggcggt tctatgcgcg gcgcagcaaa cgatctgcgc
    10501 aaccaggcgg ctgcaatggg cggtaacgtg atttatggca tcagcagccc gtcgcaggga
    10561 atgttgtcca gttttgtccc gacggatagc cagattatcg gtcaggttta taagtgcccg
    10621 aactgatgtg atccagaggc tggatacttc cagcctcgta gtaaaacaga tgagattatt
    10681 tcgcctgtgg tgcagttttg ctggtggatt gaacacgtaa gtgatgcagc gcgcgtaagc
    10741 attgatcctg ggttagcggt tgggcgttat ttgccgcaac ggtacgaaca cagacctgac
    10801 atggcacacc cgcgagtttt ttcagtcggg ggctgaaatc aagcattcgg cgagtgatcg
    10861 cctccacttc tttacccggc gttgcttcac gcgttttcac cactgcgccg ctaaattgtt
    10921 cattgccact ttcatcttta aagcggtaaa gcaccgccag ataatggttg aacaagttat
    10981 gtttccattc cggttgggaa taggtgtcat attccagatt accatcatcc tggcagtcga
    11041 catcatataa cggcaggaaa tctaacagtg aggtaatacg taacgccagt tcacgcattt
    11101 ctgcattatt aattgcatga atgattgctc gcgccagcat ttcaatctgc aattcattga
    11161 ccactaattc gcatttgctg ccatcatgta gcgttaatgc gaaattaaga atttcgccct
    11221 gatcatcggt aagttccagc gtgttaaccc gacgattgat atcggcattt ttaagctcat
    11281 caaccagaat ttctgggata ttttctgcca ttttcttaat gactttatcg cgagcttgtt
    11341 catactgaag gcgtgcagca gcatccagtt tatgcttttg atgcagacga ctttccagcg
    11401 caatcagaag gtcgcgcaat tccatggcag acataaagaa caacgattct ttattgcgag
    11461 gttctttaat tttcagcgcc agtgcaatga aattattact cttgcgaata acaccggtat
    11521 taacaccctt gatgcttatg gccatgtagg ttctccctaa ccatttctca attaaataat
    11581 taattttagt ttataagcca gataaatggg cttggtagta atagttgtta aaataacata
    11641 aatagccgtg ctcactctat ataaacagaa aagaaaggtt aattattggt gttggttata
    11701 taacgagtaa caacagcaat gtatatgaat aattaaaggt tatgcaacgg gcaaagatta
    11761 aacttccctg tttaatcagt tactgctggc gtagctggag atccagcggc gttttgctgg
    11821 gttcgccgcc aatttctcgt gccagtttag gcaccagata tcccgacacc agtgtcagca
    11881 actcacgcat aatctgccgt gcttcgtcat cactcaccat aaaatgcgcc gcgccctgta
    11941 ctttatcgag cacatgcagg taatacggca tcacgcccgc atcgaacaac gcattactca
    12001 ggtttgccag cgtttgcgcg ttatcgttca caccacgtaa cagaacgctc tggttcagca
    12061 aagtaacgcc cacccggcgc aacttagcca tcgcctgacg gaatgtttca tctacctcat
    12121 tggcatggtt gatgtgattc accagcaaga tttgcagcgt agaatgagaa aagcgttcaa
    12181 ccagcgcatc tgtgatgcgt gccgggatca caatcggcag acggctgtga atccgcagac
    12241 gtttgatatg cgggatggct tccagttgtg tgagcaacca gtccagctcg tgatctttcg
    12301 ccatcagcgg atcgccgccg gagaaaatca tctcgtccag ttccggatgc gcagcaacat
    12361 actcaagtgc agtctgccag ttacgcttgt tgccctgatt ttcnnnnnnn nnngaagcaa
    12421 tagcggcaat ttaccgcgca gccgcctttg accagcaaaa gcgcccggtt gtggtattta
    12481 tgcaacaaac ctggcactac gctgtgctgt tcttccagcg ggtcggtgga gaatccgggc
    12541 gcgacaacaa actcatcttg cgaggtaatt acctgacgca aaagcggatc gttcggattg
    12601 cctttctcca tgcgatcgat aaatgagcgg ggcacacgca gggcaaacag ctttttggcg
    12661 ctgcgtccgg ccaacagttt ttcgtccgcg tctatattca aaagacgcag aagttcatca
    12721 ggatcggtca caacatcggc aagttgcgtt aaccaatctt ctctggatgg ggtatttagg
    12781 gttacaatat gcgccatttt gtggcttagc taccaattaa caaatttcag agggccttat
    12841 ggcaacgtac tatagcaacg attttcgtgc tggtcttaaa atcatgttag acggcgaacc
    12901 ttacgcggtt gaagcgagtg aattcgtaaa accgggtaaa ggccaggcat ttgctcgcgt
    12961 taaactgcgt cgtctgctga ccggtactcg cgtagaaaaa accttcaaat ctactgattc
    13021 cgctgaaggc gctgatgttg tcgatatgaa cctgacttac ctgtacaacg acggtgagtt
    13081 ctggcacttc atgaacaacg aaactttcga gcagctgtct gctgatgcaa aagcaattgg
    13141 cgacaacgct aaatggttgc tggatcaggc agagtgtatc gtaactctgt ggaatggtca
    13201 gccgatctcc gttactccgc cgaacttcgt tgaactggaa atcgttgata ccgatccggg
    13261 cctgaaaggt gataccgcag gtactggcgg caaaccggct accctgtcta ctggcgctgt
    13321 ggttaaagtt ccgctgttcg tacagatcgg cgaagtcatc aaagtggata cccgctctgg
    13381 tgaatacgtc tctcgcgtga agtaatgcgg ttgtggtgcg gcctgcaggc tgcaccatca
    13441 ctttttcagg tcagagatga tgaaacgcct tatcgttctt gttttgcttg ccagcgcgct
    13501 gctcacgggc tgtaacaccg ctcgcggttt cggcgaagat atcaaacatc tcggcaactc
    13561 catctcccgc gctgccagct aatttttctt ctcttccgaa aaatcatcag atttccgtca
    13621 tttttggcga tgttgtctat tattaatttg ctataggcaa acataaataa cattacctaa
    13681 aaggaagacg ttatggtgaa gaagacaatt gcagcgatct tttctgttct ggtgctttca
    13741 acagtattaa ctgcctgcaa caccacgcgt ggcgttggtg aagacatttc tgatggcggt
    13801 aacgcgattt ctggcgcagc aacgaaagcg cagcaataag caataacggt acgacagctg
    13861 tgtcgtgccg tttgtttttt ctgcgatagt tacataggta atagttgaaa ttcccctgcc
    13921 acctggcaaa atatccgttc aannnnnnnn nnccgttcaa ccatcagctt tgcaggacga
    13981 cctgcaaacg cgtcttttca ccggggacgg ccccaatttt ccggagcctg atatgtcctg
    14041 gattatctta gttattgctg gtctgctgga agtggtatgg gccgttggcc tgaaatatac
    14101 tcacggcttt agtcgtttga cgccaagtgt tattaccgtg acggcgatga ttgtcagtct
    14161 ggcgctactt gcctgggcga tgaaatcgtt accagtaggg acggcttatg ccgtgtggac
    14221 gggtattggc gcagtcggcg cggccatcac cggcattgtg ctgctcggtg agtccgctaa
    14281 cccgatgcgc ctggcgagtc tggcgttaat cgtattgggg attattggtc tgaaactcag
    14341 cactcattaa ctaccgggct gctgtaccca aataaactta ctgacatcaa acccttcccg
    14401 ggtcgcgact gccagcatct cctgtttcac ttcgtcagaa atggttggcg tgcgggagag
    14461 tatccacagg tagtcgcggt ccgggccgca aaccagcgca tggcggtatt cccgatcgag
    14521 tgcaataacg ttataaccgc catagaaagg accaaagaat gacactttca gcgcagcgcg
    14581 agttggtgcg ccggtaaagt acgctttccc ttcactctgc tgccacattt ctctgtcagg
    14641 gttatagcct ttattaatga cattcaggcc gccgtcatca cgcaggctgt atgttgcggt
    14701 gactttttcc agtccacgtt caaagcggtg atcaaaacgg gcaatctcat accaggtacc
    14761 gagataacgt ttagcgtcga aattattcac tacggtcacg ccacgcggtg gcgtagggga
    14821 actgcaggca acgaccagaa atgcagctgt cgctgcggca acgagaggga gcaggcgcat
    14881 aaatgtttcc ttactggttt ttttctaagt gtagatgaca gcaaggaaaa gcggagaaaa
    14941 ggtccgaaaa ttcggacccg atgaaatttt actgtagagt gttgagaatc tgccaggcgg
    15001 cggtaactct cgctggattg ggatagtttt tgttagccag catcacgata cccagctctt
    15061 tttctggaat aaacgtgaca tagctaccaa atccgccggt cgcccccgtt ttatgtaccc
    15121 atgatgcgcg tactgcagga gttgggggcg taatcggttt tacggggcgt gctgccagtg
    15181 caattttatt gtcactgccg ttaatgatga tgtcaggatt taccggccag tccagcattt
    15241 cccagcccag accctgatac atatcgccgg tttgccagta gcgagattgt gccagctgta
    15301 tcccttgttg aagtattttc tcattgatat caaagggttt taaattgctt tgcacccagc
    15361 gggccatatc ttcaatggtc gacttcacac cataagcttc agcatctaac gcccctggcg
    15421 aaacatgtac tgccttacct tcgcgatatc cccaggcgta gttcttttct tctgcggacg
    15481 gtacattaat ccacgtatga ttgagtttga gtggctggaa gacacgagtt ttcatcgcct
    15541 gctcaaaact caaaccagac ggcttcacag ccagtgcgcc gaacaaaccg atactggagt
    15601 tggcatacag acgttgtgtt cctggagccc atgcaggctg ccagttttga tagaagcgca
    15661 gcaagtcgct tgaggatttc acctcatccg gcacctgcaa tggcaggccg ccagcggtgt
    15721 aggttgggag atgtaatagt gtgatcccat tccactgttt agcggtaagt tcaggccagt
    15781 attttgttgt gggatcgctt aacttgattt cccctcgagc aatagcgtcg ccaccaagca
    15841 cgcccgtaaa tgttttgctg accgaaccta actcaaacaa cgtttgctgt gtgacgggct
    15901 gctttttggc gatgtccgca tagccccagg taaagtaata aggtttaccc tgataaatta
    15961 ccgccaccgc cataccgggg atcttttgtt gatctataag cggggtaatt gtgcgatgca
    16021 caatatcgtt gatttgttga ggggcggcaa atgtggagca agaggcggta attaataagg
    16081 tgcagagcgt cgttttgagc ataaggtctg gtttccatac aaaacggccc gccataggcg
    16141 ggccggattt acattggcga tgcgttagat tgtaacgaca ccaatcagcg tgtcaactgt
    16201 caggatagca gccagaccgt agaaaaccca tttacccgca ggtacgtgga ttttcagatc
    16261 gtgcatcgcg tggtgcatac ggtgtaaacc acaccacagc ggcagaacga tcatcaggaa
    16321 caggaatacg cgaccaatga agctctgcgc gaacgccaga acgcgctcgt agctcagtgc
    16381 atcacccgga aacagcccta gcggcagcag aataccaacc agcaggatca tcaccggcgc
    16441 aatgatggcg ctccacatac caccggcccc gaagaggccc cagaataccg gttcgtcaga
    16501 acgctttgga tttggattaa tcatctcagg ctccttacca gtacagggca acaaacagga
    16561 ttacgatggt ggcaaccaca gttaccgccc agagactttt gataattggc tctggtccca
    16621 ttttttcgtc ttttacaatg atattggccg cttttggtgc cagctcaaac caggttttgg
    16681 tgtgcagcag ggctgccgcc agagtgatca ggttaatgat catgataacc gggttttgta
    16741 aaaagtcgac gaatcccgcc caggcttccg ggccattttt cagggcaaac agcccgaaaa
    16801 tcagttcaat gctgaaccac acagccggaa ccgccgtgcc ttcgcgcagc atgtaaaagc
    16861 gataaaacgg caattttttc caccaggtgg acgtcattgg ccgtacatac ggtttacgtt
    16921 tagtcgtcat gttgcactcc ttagcgtggt ttcagggtcg cgataagaaa gtctttcgaa
    16981 ctttctactt tgccctgctg aatggccgca gccggatcga cgtgtttcgg gcagacttcg
    17041 gagcagtagc ccacgaaagt acagctccat acgccgttct ggctgttcaa ctgcgccata
    17101 cgctccttct taccgtggtc gcggctatct tcgttataac gatgcgccag cgtaatggca
    17161 gccggaccga tgaactctgg gttcaggcca aactgcgggc acgcggcgta gcacagacca
    17221 cagttgatgc aaccggagaa ctggtgatac ttcgccatct gcgccggggt ctggatgtta
    17281 gtaccctgat ccgcggtgcg ggagttgccg atgatgtacg gtttgatcgc ttccagactt
    17341 tcgatgaagt gggtcatatc gaccaccaga tcgcgttcaa tcgggaagtt agctaacgct
    17401 tcaaccttca taccgtcggt gtaatcacgc aggaaggttt tacatgccag ttttggcacg
    17461 ttgttaacca tcatgccgca ggagccacaa atcgccatac ggcaggacca gcggtagctc
    17521 aggtccggtg ccaggttgtc tttgatgtag cccagcgcat ccagtaatga ggtagttgcg
    17581 tcataaggca cttcatagaa tgcgctatgc ggtgcggtat cgacttccgg gttatagcgc
    17641 accacctcaa tcttcaggtt tttcatctca gccattcgcc ttctccttct tattggctgc
    17701 ttccgcctta tcggctgcat ccgcttcgcc accgtaaaca cgtttagctg gcggcagcgt
    17761 agtaatcttc acgtcgctgt actccaggcg agtcgtgcca tcagcatcgc ggaaggcgag
    17821 ggtgtgtttg aggaagttga cgtcgtcacg ctcggtgcaa ccttcgtcca gacgctggtg
    17881 cgcgccgcgg gactctttac gtgccattgc ggagtgcgcc atacattcag caacgttcag
    17941 accgtggccc agctcaatgg tgtagagcag gtcggtgttg aacacgctgg aagtgtcggt
    18001 gatgcgcacg cgcttgaagc gttcctgcag ctctgccagc ttgtcgatgg ttttctgcat
    18061 cagttccggc gtacggtaga taccgcagcc ttcttccatg gccatgccca tttcgtcgcg
    18121 gatcttagcc cagttttcgc cgccatcctg gttaaccaga tctttcagac gttgttcaac
    18181 gccagctgcc tgcgcttcaa ttgccgcttc gttgccatta ccggcagttg ctgcacgctc
    18241 tgtcgcttgt tcaccggcca gacgaccgaa gaccaccagt tccgccagcg agttggagcc
    18301 cagacggttc gcaccgtgca gaccaacaga ggaacattca cccacggcga acagaccttt
    18361 aatgcgggtt tcacagttct gatcggtttc gataccgccc atggtgtagt gtgcggtcgg
    18421 acgtaccgga atcggttctt taaccggatc gacgccaacg tacgctttcg ccagttcgca
    18481 gatgaacggc agacgttcat gcagtttttt ctcgccgagg tgacgcaggt cgaggtaaac
    18541 cacatcgcca cgtggcgtgg agatggtgtt gcctttacgc cattcgtgcc agaaggcctg
    18601 agaaactttg tcgcgtggac ccagttccat atatttgttt ttcggctcgc ccagcggagt
    18661 ttccgggccc atgccgtaat cttgcagata acggtagcca tttttgttga ccagaatacc
    18721 accttcaccg cggcagcctt cggtcatcag gataccggaa cctggtagac cggttgggtg
    18781 atactgaacg aattccatgt cacgcagcgg aacgccgtgg cttagcgcca tacccatacc
    18841 gtcaccggta acgatgccgc cgttggtgtt gtaacgataa acgcgacccg caccgccggt
    18901 agccataacg accgcgttag cacggatctg caccagcgtg ccttccatca tgttcattgc
    18961 taccaggccg cgaacatgac catcatcaac cagaatatcc agcacgaaat gttcgtcaaa
    19021 acgctggatc tgcgggaatt gcagagaggt ctggaacagc gtgtgcagca tatggaagcc
    19081 ggtcttatcg gcggcgaacc aggtgcgctc gattttcatg ccgccgaagc gacgtacgtt
    19141 gacgctacca tccgggcgac ggctccatgg gcacccccac agttccagtt gggtcatttc
    19201 ggttgggcag tggtggacga aataatccac gacatcctgc tcacacaacc agtcgccacc
    19261 cgctactgta tcgtgaaagt gatattcgaa gctgtcatga tcctgcgcga cagcggcgga
    19321 gcccccttct gcagcaacgg tatggctacg catcgggtat acttttgaga ttagtgcgat
    19381 ttttgcattt ggatttgcct gcgcggcagc aattgcagca cgtaatcccg cgccaccggc
    19441 gcctacaatg gcaagatcgg cttgaaaggt ttgcacgaca ttcctccaga ttgtttttat
    19501 cccacagccc cgtacttcag ggtaagtgcc tgaaagttac ggtctgcgaa cgctattcca
    19561 ctgctccttt ataggtacaa cagtatagtc tgatggataa gtctgaaatt tgacgagatc
    19621 gattttttta gtgcgcaaga gggctaaatt atcactgaag atgattaatt taattactaa
    19681 accatcagat cgtgcttttt ttagtcactg cccaccgttg tgttattcct gcgtaaaatt
    19741 tgtctcgccg tcgcgttgcg agtagacttc gtgcccttgt caaaaactgg agatttaact
    19801 atgagcgaaa cggcatcctg gcagccgagc gcatccattc ctaacttatt aaaacgcgcg
    19861 gcgattatgg cggagatccg tcgtttcttt gccgatcgtg gagtgctgga ggtggagacg
    19921 ccttgtatga gccaggcgac ggtaaccgat attcatttgg tcccgtttga gacacgtttc
    19981 gttggccccg ggcattcgca ggggatgaat ctctggttaa tgaccagccc ggaataccat
    20041 atgaagcgcc tgctggttgc cggttgtggg ccggtattcc agctgtgccg cagcttccgt
    20101 aatgaagaga tggggcgtta tcacaaccct gagttcacta tgcttgagtg gtatcgaccg
    20161 cactatgata tgtaccggtt gatgaacgag gtggacgatc tcttgcaaca ggtgctggac
    20221 tgtccggcag cagaaagcct ttcttatcaa caagctttct tacgttatct ggaaattgac
    20281 ccactctctg ccgacaaaac gcaactgcgg gaagtggcag cgaaactgga tttgagcaac
    20341 gttgccgata ctgaagaaga ccgcgacacg cttctacaat tgctgtttac ctttggcgta
    20401 gagccaaata ttggcaaaga aaaaccgacc tttgtgtacc actttccagc cagccaggca
    20461 tcactggcgc aaatcagtac cgaagatcat cgggtcgctg aacgctttga ggtttattat
    20521 aaaggtattg agctggcgaa tggtttccat gaattgacgg atgcccgtga gcagcaacaa
    20581 cgctttgaac aagataaccg taagcgcgcg gcgcgcggtt tgccgcagca ccccattgac
    20641 cagaatctga ttgaagcctt gaaagtcggt atgcctgact gttccggcgt ggcattnnnn
    20701 nnnnnntctg gtgatgttgg cgctgggcgc ggagacactg gctgaagtca tcgcctttag
    20761 cgttgaccgg gcataattct gaaaaattac tgaattaaca gcgaagaatg gcgtgataag
    20821 cgccattctg tagcatatgt tttcattttt ccgctaaaga ccacaattta agactggata
    20881 tttgatatca tccaggtatc aattctgtat tggtttttac gctgcccgct caatgttggg
    20941 cagtgcagtt cagtgggcgc gttttataca cgcgctgaaa tgaaggatgg tttcatgcct
    21001 cactcgataa aaaagatgag tctgatagga ctcatattga tgatctttac ttctgtattt
    21061 ggatttgcca atagcccatc ggcttattac ttaatgggtt atagtgcgat tcccttttat
    21121 atattttctg cattgttatt ctttattcca ttcgccttaa tgatggctga aatgggagct
    21181 gcttatcgca aagaagaagg cggtatctat tcctggatga ataatagtgt cggaccacgt
    21241 tttgccttca ttggtacgtt tatgtggttt tcctcttata tcatctggat ggcgagtacc
    21301 tccgcgaaag tttgggtacc gttctcaaca ttcctctacg gtagcgacat gacccagcac
    21361 tggcatattg ccggactgga gcctacgcag gtggttggtc tgctggcagt ggcatggatg
    21421 attctggtca ccgtcgttgc ttcaaaaggg attaataaaa ttgcccgcat tactgcggtg
    21481 ggcggtattg cagtaatgtg tctgaattta gtattgctgt tagtaagcat tactattttg
    21541 ttattaaata gtgggcattt cgcgcaggat attaatttcc ttgcatcacc gaacccaggt
    21601 tatcagtccg gtctggcaat gctatcgttt gtggtatttg ccatttttgc ctatggcgga
    21661 attgaagcgg ttggtggtct ggttgataaa acggaaaatc cagaaaagaa ctttgccaaa
    21721 ggtattgttt ttgccgctat tgttatttca atcggttatt cgctggcaat atttttatgg
    21781 ggcgtcagca caaactggca gcaggtatta agtaatggtt ccgttaacct cggcaatatt
    21841 acctatgtgc tgatgaagag cctcggggtg acgctgggta acgcactgca tttgtcacct
    21901 gaagcgtcat tgtcgctggg cgtatggttt gcgcgtatta ccggactttc gatgttcctc
    21961 gcttataccg gtgcgttctt tacactttgc tattcaccgc tgaaagccat catccagggg
    22021 acgccgaaag cattgtggcc ggaaccgatg acgcgcctga atgcgatggg gatgccgtct
    22081 atcgccatgt ggatgcagtg tgggttggtt actgtcttca tcctgctggt ttcgtttggt
    22141 ggcggtaccg catcggcgtt ctttaacaag ctgacgctga tggcgaacgt gtctatgacg
    22201 cttccttacc tgttcctcgc gctggctttc ccgttcttta aagcacgtca ggatctcgac
    22261 agaccatttg tgattttcaa aacgcgtatg tcggcaatga ttgcgacagt ggttgtcgta
    22321 ctggtggtga catttgcgaa cgtcttcacc atcattcaac ctgtggttga agccggagac
    22381 tgggacagca cattgtggat gattggcggc cctgtcttct tctcgctgtt agcgatggcg
    22441 atttaccaga actattgcag tcgcatggcg aacaaacctg agttagctct cgactgatca
    22501 tatgaaatcc tgcaactcat cgtctctccg gcgatgagtt gtcaggaagt aatgcgtatg
    22561 gatgatactc tccggggcca gtgataactg aagatgatgt taagctgcta aatgagccac
    22621 gttgacatca tttggcagct tcatgcatta caaacttccc gcctgacgac ctttgcccgc
    22681 agaagttaat gttctccccg tttgcttacc attcaggctt tccagacgca tctggaaggg
    22741 cgggaatggc atatcaatac cgtgggcatg gaatcctgcc agaatcagct ggtgaatctc
    22801 atggcgtagt ggcatacggt gacccatctc agcggcgtaa atacgcagct cgaaaatttg
    22861 aatcccctgt tgcagatcca ccaggaagac ttccggtgct gggttgtcga tcaccagtga
    22921 gcagcgacgc gctgcggtga gcaggatttc cgtcacttct tcactgttgg catcggcagg
    22981 ggccggaatt gtcaacacca cacgcgtgac cgagtcagag agcgaccagt tgataaactg
    23041 ctcggtaata aacgccttgt tcggcacgat aatttctttg cggtcccagt cgctgatggt
    23101 ggtggcgcgg gtgttaattt tcgtcacact accggtaaga tcgcggatcg ttaccgtatc
    23161 gccaatgcga atcggttttt cgaacaggat gatcaggcca gagataaagt tggcgaaaat
    23221 ttcctgcaaa ccaaaaccga gaccaacacc gagcgcggca accagccact gcaatttcga
    23281 ccactcaata ccaatcattg agaagccgac cagcccgcca atcagcatca gcagatattt
    23341 ggtgatggtg gtgatggcgt agcccgtacc cggcgttaaa tccaggtgct gcagaatcgc
    23401 cagttccagc agcgcgggca ggttgcgcac cagctgcgtg gtgatgataa acaccagaat
    23461 ggcaatcagc accgcaccga gggtaatcgg ctccaggctt tctacgccct gtaccgtgga
    23521 agtgacatcc cacagcgaaa tattttcgag gaagccgaaa gcggaatgga tttctgacca
    23581 cagcacaatg accgaaagca gggcgatcaa catcaaaatt gagcgcacca gccgcaagga
    23641 ttgcgcactg atggcatcga gatcgacttc gctttcatcg acttcaattg ctccttccgg
    23701 gctactgtga tgatgcgctt cctcttcgcc acgcgcacgc tgcgctaaca tctctgcccg
    23761 gcgatgcttc gcccggtcaa acgccagcct gcggcgctgg atcagcatcc agcggcggat
    23821 aacgtgataa accaccagta gcaggaacca gatggcaacc gaggtttcaa gcctcgctaa
    23881 cagtgcctgc gccgttgcca gataacccac cgccgacgcc agaatggcaa ccagcggtgc
    23941 gccaatcatc atgttccaca gcatatggtt ggtaatgttg tcgccgctgc cttctttgtt
    24001 gagatacagc gggatcccgg cctttttcag gctgagggtg accaccgcca gcgcaccgca
    24061 aatgaggata aagcaaagcc gtcccagcga accggaannn nnnnnnncag cggcacaatg
    24121 agcccgatgc tcatcaggta gtaacgcatc ccacgggaaa ctcgttcgcg cggccagcca
    24181 aaatgagcga taaacaagcc gttcgggcga gcaaaggtgg cgcaaatcat cactacccac
    24241 agcagcggca cggtggctgt tacgccatca ccaatcgcga ccgccagcgg ataaggccac
    24301 gcctcgcgca agccgtaacc cagtgtcatc cacagcacag gcagcggtga cgcgacgaga
    24361 atcgaccaga aaagagtgcg taacgtcagc cagaagtgat cctgagtcac tttgccgact
    24421 ttcgccgccg aacgttcaag aaaacgggtg aaatagcggc gcgagtaaat actgcaaccg
    24481 accagaatca acgcgccaaa cagcggcaaa atcgtctctt tactggtcag catcatcaca
    24541 ctggctttgc ccaactgact gaaggtgtcc agcgaaatga gacgacgtag atcctgggcg
    24601 atttccagcg gccaggcgat ggtcatcggg cgcacgtcag aggtccagaa cagatagcgg
    24661 tgcgtcgctt cgttcacctc tttcagcgca tcctccagtt gcccgttgga gactttcagc
    24721 ttggtcagct ccagcagtag cgtgtcgcca ccctgcaaca atgagttcag caactcacgc
    24781 tgagtgcgta gctgtgcttc cagaatacgg ttttgctcag cggtcagtgg ctgaccgtcg
    24841 gcctgatgga tttgccgtag cagcggctgt ttattaagca gatcctcata acgtaaccgt
    24901 tgcacacgta actgcgccat ttcggtatca agctgttgtg gttttggcat ttccggtagc
    24961 cgtgccacct gcgcccgcaa cgcttcgccg agcaggttag atgatcccag ccattgcgac
    25021 tgttcacgca gcgtattcaa cgcctgccgg acctgtaacg tctggctggc agcctgacgc
    25081 tgttgcgagg caacgagatc catccgctgc gcctgttgat tcagtgccgc cgatagttcg
    25141 cggttaattt taaactgggc gacgatatct tttggcaaat cggcgctact ttccgccagt
    25201 tgctcggtac tttccagagc ccgttccgct tcctgttggc gttgactatt caactggttg
    25261 cgtagcgctt gcaaatatgc atccagctgc tggctctctt tttccgccag ctcagagcgc
    25321 aagcgcgcta attcctggcg gttattggca gataactgcg ccagctccag ttcatcaacg
    25381 agcgctttca gacgtgcgga gtcagactgt aacgcgaaat tttgtgcctg attgagcggg
    25441 gtattgccgg taagcgtccc caggcggcgc tcgatttcat tcaactgacg gcgggcatcg
    25501 gtttgctgtt gcggcagttg attcagcgaa tcggcaatct cgcgggcgcg ctcctgctct
    25561 tgctgggcct gacggctttt atccagcaac tgactgctga tctggagaat ttcctgattc
    25621 agcgcatcgt tagacattcc cggcgacacg ctgcgtggct cgtcacgcat gttgtttaac
    25681 tgcgcgcgca gagtggcgga aagtttcgga taattatcaa tgacttcttg atattgcttg
    25741 atgcgctcaa gggaaccttt tcgttcctca agcgcattta aggcagactg gagcgcctct
    25801 acgacttccg gctgcgcagg tttcgccgct tttgcctgct ccagttcctg ggtgatttgt
    25861 ttgctatcgg gggccgtcgc ggcgtacgcc ccccaactga ggcaccaggc catcagaaaa
    25921 gtgataatca ggcgcacgtc agcgtttcct ttgatggatt agacctggtc ttttttgtcg
    25981 tcaaccaatg ggctggcgtc gtgttctgct tcgatctctt cagcaggaag cggggctggt
    26041 tcagcgtctg gcgtaacaaa ggtttcggta gatactgcca gcggctggcc aattttcgtg
    26101 acagacaggc tttccagttg ctcaaccagg ttcactttac ctggtgcaaa caggttgata
    26161 acggtagagc caagtttaaa gcgacccatt tcctggcctt tcagcaatgc cacagaatcg
    26221 tcgttttccc cggcaggcca ggtccagcgc ttgatgatgc cttcgcgcgg cggcgtaatg
    26281 gtgcccgccc agaccgtctc aatgctgcct acaatcgtcg caccgaccag aatctgcgcc
    26341 atcgggccaa attcggtatc gaacaggcag atcacgcgtt cgttacgggc aaacagattc
    26401 ggcacgttct gagcagtgag atggttaacg gagaagagat cgcccggcac gtagatcatt
    26461 tcacgcagaa taccgttgca cggcatgtgt acgcggtggt agtcacgtgg ggagaggtaa
    26521 gtggtcacaa acgtaccgtt acggaagaga tccgccatca gatagttgcc tgccagcagg
    26581 gcttcgaggc tgtagttgtg gcctttggct tgcaggattt tatcttcttc gattttaccc
    26641 aactggctga taacgccatc ggcaggcata accagtacat tcggatcggt atcgattggg
    26701 cgtacttcgt cacgcagcgg acggacaaag aattcgttaa aggtgcggta gctggcggtg
    26761 tccggctttt gcgcctcttt catgtcgacc ttgtagtatt taacgaacag atcgataacc
    26821 agttttgtca gccatcctgc ccgcttgctt gcgccccaac ccgccaggcg agtaagccat
    26881 agtttcggca gaatgtactg tagcgaaagt ttaaatgaat ttaacaaggt agcctccagg
    26941 ccattgtttt gtcgttcctg atccggccta catgccggat cctgaaaaaa aggggacgat
    27001 tctaacgacg gttagcttaa ttgtcagtca cccgtatcag aaaagttttt acgcgttttt
    27061 acctgcgcca tgctttccag aatacggtga tagttttcga aacgggtttc cgcgattttc
    27121 ccttcctcaa ccgcttcccg gatagcgcag cccggatcgg tatcgtgttt gcaatcgcga
    27181 tatttacaca gacctaaata gtcatggaat tcgacaaagc cctgagtgat ttgttccggc
    27241 tccaggtgcc agagaccaaa ctcacgcaca cctggggaat caatcacatc accgccgtgc
    27301 gggaagtgat acagtcgtgc ggcggtcgtg gtgtgctgac caagccccga gttgtcagag
    27361 acatcgttgg tcaggatctc tttttgcagc cccagcaacg cgttcagcag gctggatttg
    27421 ccaacgccag actgcccggc aaaaatgctg atgcgcccgg tcaacgcctc ttccagcggt
    27481 ttcagcccat cctgagtatg actggaaacc atcaatacgc gataaccgat attgcggtag
    27541 atatccatct gctcgttgac gaactccatg ccttcgtcgt ccagcaggtc tatcttgttg
    27601 agcacaataa tcggctcaat ctgcaaggtt tcgcaggcca ccaggtaacg gtcgataata
    27661 ttgagcgaca gctccggcaa aatggcggag acaatgacaa tctggtcgat gttggcggca
    27721 ataggtttca cgccatcgta gaagtccggg cgcgttaaca ctgaagtgcg ctcatgcacc
    27781 gcttccacga tccctttgac atttacgcct tccgccgccg gtttacccgg acgccagact
    27841 acgcggtcgc cggtcaccaa cgaacggatg gtacggcgaa tattgcagcg gtgaacgtcg
    27901 ccatcggcgg attccacatc agcgtgcata ccaaagcggc tgatgacgat accttcatca
    27961 ggctcgccaa acagattgtc gtcgtagtcg ggcttctcct tagacgtttt aagacgacgc
    28021 tggtgattgg cgttcacgcg gcgctgctgg cctttggaga gtttattttt actcaatcgt
    28081 acagactcct ggtcgcccct gatgggcaaa acatctatga tacacgcaat tgtggatcaa
    28141 tatagtcact gtgaatgggt ggaaaatagc atgagtgcca atgaaaacaa cctgatttgg
    28201 atcgatcttg agatgaccgg tctggatccc gagcgcgatc gcattattga gattgccacg
    28261 ctggtgaccg atgccaacct gaatattctg gcagaagggc cgaccattgc agtacatcag
    28321 tccgatgaac agctggcgct gatggatgac tggaacgtgc gcacccatac cgccagcggg
    28381 ctggtagagc gtgtgaaagc gagcacgatg ggcgatcgtg aagctgaact ggcaacgctc
    28441 gaattcttaa aacagtgggt gcctgcggga aaatcgccga tttgtggtaa cagcatcggt
    28501 caggaccgtc gcttcctgtt taaatacatg ccggagctgg aagcctactt ccactaccgc
    28561 tatctcgatg ttagcaccct gaaagagctg gcgcgccgct ggaagccgga aattcttgac
    28621 ggctttacca agcaggggac gcatcaggcg atggatgata tccgtgaatc ggtggcggag
    28681 ttggcttact accgcgagca ttttatcaag ctgtaaaatt tttcctggtc acgtaagcgc
    28741 cggaatccac ttgcggcggc gtgattttga cgctaaattg aacgtcttgc tgataatttc
    28801 agcacttgag ataaaaactc aaaaaaaatt ttttaggggg gttgcagagg gaaagatttc
    28861 tcgtataatg cgcctcccgt aacgacgcag aaatgcgaaa attacgaaag caaaattaag
    28921 tagtacgcgg gaatagctca gttggtagag cacgacctt
//
LOCUS       DAWDRC010000003        26962 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_972_length_26962_cov_2.515591, whole genome shotgun sequence.
ACCESSION   DAWDRC010000003 DAWDRC010000000
VERSION     DAWDRC010000003.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26962)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 26962)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..26962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_972_length_26962_cov_2.515591"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10509..10518
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15926..15935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    24711..24720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6755 a         7223 c         6534 g         6420 t
ORIGIN      
        1 gatatatggg agatggcggt agaatgaccc gttttcaatc ccaacgtaag caaaaaatac
       61 gatgaatctg agtactaaac aaaaacagca cctgaaaggt ctggcacatc cgctcaagcc
      121 agttgttctg cttggcagta atggtttgac cgaaggggtg ctggccgaga ttgaacaagc
      181 gttagagcac catgaactca tcaaggtgaa aatcgccacc gaagatcgcg aaactaaaac
      241 cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt aatgtacagg tcatcggtaa
      301 aacgctggtg ctttatcgcc caactaaaga acgtaaaatc tcgctgccac gctaagatta
      361 tcctaaagtt acacacattc gctgtgtaaa acgagggttt ttccgcaggc aggagagcaa
      421 aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg cctttttcct ttctttacaa
      481 tacatcaaca tcttgagtat tgggtaattc ttacaggtat tccaccttaa ttacttcaaa
      541 ttctacttcg ccgcccggcg ttttgatgac cacaacatca tcttcttctt tgccgatcag
      601 gccacgagca ataggcgagt ttacagaaat caggttttgt ttaaagtcag cttcgtcatc
      661 gcctacgatg cgataggtct gttcttcatc agaatccaga ttcaaaaccg tcacggtagc
      721 gccaaaaatc acgcgaccat tgtttggcat tttggtgaca tcaattacct gcgcgttcga
      781 cagcttggct tcgatatctt tgatacggcc ttcgcaaaaa ccctgctgct cacgagctgc
      841 gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc gcttccgcga tagcagcaat
      901 gatttcagga cggcgcacag atttcagaaa atccagctct tcgcgtaatt tttcagcgcc
      961 gcgtaaggtc atcggaatag cttgcatttg ttatacctct tgaatattcc tgatggggca
     1021 agtcttcacc caccccggct gttcggcctg cccggcataa ctcctcaccg ggaccagaag
     1081 caaaaaaata ccgacccggg tataagtccc aggtcagcta caattcacat tttgatagtc
     1141 attttaccct ggagttcccg aagggtcatc gtttacttta tagggcgttg cgccgtagta
     1201 tgacggctcg attccaggtt gttagcgcga gattatgcga ttttccagat ttatcatcgg
     1261 attgaccagc tgtatagcgt tcagtgttca ggccgcaaat gttgatgagt acattactca
     1321 actccccgca ggtgccaacc ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc
     1381 tattgattac cacagtcagc agatggcgct acccgccagt acgcagaaag tgattactgc
     1441 gctggcggcg ttgattcaac tcggccccga ttttcgtttt accacgacgc ttgaaaccaa
     1501 aggcaatgtg gaaaacggca tacttaaggg tgacttagta gcgcgatttg gtgccgatcc
     1561 gacgttaaaa cgtcaggata ttcgcaatat ggtcacgact ttgaaaaaat ctggcgtcaa
     1621 ccaaatcgat ggcaatgtgt tgatagatac ctccattttc gccagccacg ataaagctcc
     1681 cggctggcca tggaatgaca tgacacaatg ctttagcgct ccgcctgccg ccgccatagt
     1741 tgaccgcaac tgtttctccg tctcgctcta cagtgcccca aaacctggtg atatggcttt
     1801 tatacgcgtg gcatcttatt accccgttac gatgttcagt caggtacgca ccctcccccg
     1861 tggttctgcc gaagcgcaat actgcgaact ggatgtcgta ccaggtgacc tgaaccgctt
     1921 tacgctaacg ggatgcttgc cacaacgttc tgagccgctc ccgttggctt ttgccgtgca
     1981 ggatggtgcc agctatgccg gggcaattct gaaagatgag ttaaaacagg cgggtatcac
     2041 ctggagcgga acgctgctgc gccagactca ggttaacgaa cctggaacgg tagttgccag
     2101 taaacagtcg gccccgctgc acgatctgct taagattatg ctgaaaaagt cggacaacat
     2161 gattgccgat acggttttcc gcatgatcgg ccatgcgcgc ttcaatgtgc ctggaacatg
     2221 gcgggccgga tcggacgccg tgcgtcagat cctgcgccag caagccggtg tcgatatcgg
     2281 aaacaccatt attgccgatg gttcagggct ttcgcggcat aacctgattg cccccgccac
     2341 catgatgcag gtgctgcaat atattgccca acacgacaat gaacttaact ttatctccat
     2401 gctgccgctg gcgggctatg acggctcttt gcagtaccgt gcaggtctgc atcaggcggg
     2461 cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag ggggtatata acctggcggg
     2521 attcattacc acagcgagcg ggcaacgaat ggcgtttgtg caatatcttt ctggctatgc
     2581 agtagaaccg gcggatcagc gtaatcgccg tattccgtta gtgcgttttg aaagccgttt
     2641 gtataaagat atttatcaga ataattagcc taaagagaaa ccccggcaca tggccggggt
     2701 tttgctgatt aacgcttgta aatgaactca acgccttctt cgtcgtcttc gtcccagtcg
     2761 tcatcccagt cctcatcatc ctcttcagca atctcttcaa gctgctgacg gtgataatca
     2821 tcccacatga attcgacttt ctctggctgt ttcgcttctt cagcctgcac gaccgggttt
     2881 tcaatgataa aggtcatcac atcccagcag agatctttca cgcccagtcc gctcgccgca
     2941 gagatcagat aatatttatc ttcccagccc agcgcttcag cgatcgcttt cgctttctct
     3001 tcggcttcta ccttatccag cagatcgatc ttgttgaaga ctaaccaacg cggtttcgcc
     3061 gccagatcct ggctgtattt ttccagctca ctgataataa tacgcgcgtt ttcaaccgga
     3121 tcggtgccgt caatcggatc gatatcgatg aggtgcaaca ggacgcggca acgttccagg
     3181 tgcttcagga agcgaatgcc cagacctgcg ccttctgcag cgccttcaat cagtcctgga
     3241 atatcggcaa caacgaagct cttttcgttg tccatacgca ccacgcccag gcttggtacc
     3301 agagtggtaa acggataatc cgccactttc ggtttagccg ccgagacagc gcggataaat
     3361 gtcgatttac ccgcgtttgg catccccaac ataccgacgt cagccagcag catcagctcc
     3421 agcagcagct cacgcttatc gcccggcgta ccgttagttt tctgccgtgg tgtacggtta
     3481 actgacgact tgaaacgggt attgcccaga ccgtgccagc cgcccttagc aaccagcaga
     3541 cgctgaccgt gtttggtcat atcgcccatg gtttcaccag taccctggtc gattacacgc
     3601 gtacctaccg gtactttaat cgtgacgtct ttaccgcgct taccggtaca gtctcggctt
     3661 gcaccgttct gaccgcgctc tgcacggaaa gatttttcaa aacgataatc gataagcgtg
     3721 ttcaggttct cgtcggcttc catccatacg tcaccaccat caccgccgtc gccgccatcc
     3781 gggccgcctt tcggaatata cttttcgcgg cggaagctca cgcaaccatt accgccatca
     3841 cctgcaacga ccagaatcga tgcctcatca acaaacttca ttttattctc cgtaaatcat
     3901 tcgcctgcgc ggggttgcga taccaccgtt gtatgcttac gtaatccgcc ccaaatacga
     3961 tgaccaatgg cggaatacat cgcgcccgca accacgacaa acgcaccgag ataacctaaa
     4021 aggtttaaca tcggtctggc gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa
     4081 aacagcgtaa acagtggggt gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga
     4141 gccatcgctt ccgccagggc gccatatcct accaatgtat tcagtccgca aaaaattaaa
     4201 catgcgagct gccagtggct aagctgcgct atcactccag gatttgccag agggaagagc
     4261 gcaattgtac ataaagtgta cagtaaaaac aggatctgcg gtgaggccag ccgacgcaat
     4321 aaaacctttt gcgccacgcc ataactcacc caaaccgtcg ccgcaccgac cccaaagata
     4381 actccccagg tgtaatcggt gagctttgta aatatctcga ccagactggt gttaaaaaac
     4441 atcaccaggc cgctcaggag catcaatgcc cctacaacct gagtactgcg cattttctct
     4501 ttcaggataa atacgctggc aaccatcatg ccaactggcg agagttgccc aattacctgc
     4561 gaagcggtcg gactcaggta ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc
     4621 ccggcggtcg ccactgctaa caaaatcaac cagcgtggct tacgaaacac gcgtaatggc
     4681 ggcaaccgct tcttcaccgc aagaatggca ccaaggccaa tactcgccat caagaaacgg
     4741 taaaacacga ttgtcggagg ttccatcacc tccagcacct gcttcattgc gattggcaac
     4801 gccccccagc aaattgctgt ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc
     4861 ataccgtatt ccctgcaaag cgcatatgcg gattaacgtc gcaccatgcg catttttacc
     4921 ggttatcgaa tgtaaaaagc cccgcaacgt gttgcggggc tttcatccgt taccgggacg
     4981 cgaaaaactt attcagcttc gatgctgata aatttacggt ttttcgggcc tttaacttcg
     5041 aatttcactt taccgtctgc tttagcaaac agagtgtggt cacgaccgca acctacgtta
     5101 gcgccagcgt ggaatttggt accacgttga cgaacgatga tgctacccgc cagaacggat
     5161 tcgccaccga aacgcttaac gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg
     5221 gagccgccag cctttttatg tgccatttga aatctctcct caggtcttag gcgctgatgc
     5281 cagtaatttt cacatcagtg aaccactgac gatggccctg ctgcttacga tagtgtttac
     5341 gacgacgaaa cttaacgatt ttaactttct cgccacgacc gtgagcaaca acttcagctt
     5401 tgattacgcc gccatcaacg aaaggaacgc cgattttgac ttcttcaccg tttgcgatca
     5461 tcagcacttc agcgaactca acagtttcgc cagttgcgat gtccagcttt tccaggcgaa
     5521 cggtctgacc ttcgcttact cggtgttgtt taccaccact ttggaaaacc gcgtacatat
     5581 aaaactccgc ttccgcgcac accttttcaa tgattcagag tgcgctataa atattcacaa
     5641 tagggcgcga atattacgca aaacgcacgc ctttgacaag tgctacagtc aatacacgaa
     5701 gaaaaaaaac acaacttgta cggtaacgtt tatctgtgcc attttttcag tacaatcacc
     5761 ctatattcct aaccataaac cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa
     5821 aagcccggct tttgcgatga atttagaaaa aatcaatgag ttaaccgcgc aagatatggc
     5881 gggtgttaat gcggcaatcc ttgagcagct taattccgac gtccaactga tcaatcagtt
     5941 aggctattac atcgtcagcg gcggcggtaa acgtattcgt ccgatgattg ctgtactggc
     6001 tgcacgagct gttggctatg agggaaatgc gcatgtcacc attgctgccc tgatcgagtt
     6061 tatccacacg gcgactctgc tacacgacga cgttgtggat gaatcagata tgcgcagggg
     6121 taaagctacc gccaacgccg catttggcaa tgccgccagc gtgctggtag gcgattttat
     6181 ttatacccgc gcgttccaga tgatgaccag cctcggttca ctcaaagtgc tggaagtcat
     6241 gtcagaagcc gtaaacgtca tcgcagaagg tgaagttctg caactgatga acgttaacga
     6301 tccggacatc actgaagaaa actacatgcg cgttatctac agcaagactg cgcgtctgtt
     6361 tgaggctgcc gcgcagtgtt ccgggattct agctggctgt acgccggagg aggagaaagg
     6421 cctacaagat tatgggcgct atctcggcac tgctttccag ttgatcgacg atttactcga
     6481 ttacaatgcc gatggcgaac agttaggtaa aaacgtcggc gacgatctga acgaaggtaa
     6541 accgacgctg ccgctgctgc atgcgatgca tcatggcaca ccagaacagg cacagatgat
     6601 tcgtaccgcc atcgaacagg gtaacggtcg ccatcttctg gaaccggttc tggaagcaat
     6661 gaacgcttgt ggatctcttg aatggacgcg tcagcgtgcc gaggaagaag cagacaaagc
     6721 catcgcagcg ttacaggtgc tcccggacac cccgtggaga gaagcactta tcggcctcgc
     6781 gcacatcgct gttcaacgcg atcgttaatc ccctcccctc atcccgcgta gtgcgcggga
     6841 tgattccagt aaattccata aaacacttat tcagctctat aactcctgaa aaacagggct
     6901 gtgtatcttt atatattctg aatattcaca ctctttacag gaacttttta gagcaatagg
     6961 ccatcaggag tatagtgatg ctcgacagaa acagaagaag tgttctgaat gaaagcgact
     7021 aacttaagga gtgaggaaag atggaaagta atttcattga ctggcatccc gctgacatca
     7081 ttgcaggttt gcgcaagaag ggaacgtcaa tggcggcgga gtctcgcaga aatggtttga
     7141 gttcctcaac gctggcgaac gcattatcac gcccatggcc gaaaggagag atgattattg
     7201 cgaaagccct gggaactgac ccctgggtta tctggccatc acgctaccat gatccgcaga
     7261 cccatgagtt tatcgacaga acgcagttga tgcgtagcta cactaaaccg aaaaaatgag
     7321 tggcctggcg gtagccccgc gaacggggct gccagctctc agacaattac tcgcctttca
     7381 cacgctcaat atttgcacct aaagcgcgta gtttgtcttc aatgcgttcg tagccacgat
     7441 cgatgtgata aatacgatca accaccgtcg tcccttccgc aatacagcca gccagcacca
     7501 ggcttgctga tgcacgcaga tcggttgcca taacctgtgc gccagaaagt ttttcaacac
     7561 cgtgacaaat aacggtattg ctttcgattt cggcgtgcgc gcccatacgg ctcagctctg
     7621 gcacatgcat aaagcggttt tcaaagaccg tttcggtgat gaatccggtc ccttctgcca
     7681 ccagattcaa cagcgtgaac tgggcctgca tatcggtagg gaatgccgga tgcggcgcgg
     7741 tacgtacgtt aacagccttc ggacgtttgc catgcatatc caggctaatc cagtcttcgc
     7801 cgacttcgat gtccgctcca gcgtcacgca gtttcgccag cacggcgtcc agagtatctg
     7861 gctgcgcgtt acggcagata attttgccgc gagagatcgc cgccgccacc aggaaagtac
     7921 cggtttcgat acgatccggc agcacgcgat agacaccgcc gcctaaacgt tccacacctt
     7981 cgatgacgat acgatcggtg ccctgaccgc taattttcgc acccagcgta atcaggaagt
     8041 tcgcggtatc gacgatttcc ggttcacgcg ctgcgttttc aataatcgtg gtgccttccg
     8101 caagggttgc agcacacatg atggtcaccg ttgcgccaac gctgacttta tccatcacga
     8161 tatgtgcgcc tttcaaacga ccatcgacgg aagctttaac gtaaccttct tccagtttga
     8221 tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc aaccggacgt gcgccgatcg
     8281 tacaaccgcc aggcagtgaa acttgcccct gaccaaagcg cgctaccagc ggccccagcg
     8341 cccagataga agcacgcatg gttttaacca gatcataagg tgcgcagaat acattaacgt
     8401 cgcgggcatc aatatgcaca gaaccattac gttctacttt cgcacccagc tggcttagca
     8461 gcttcattga tgtatcgacg tcttttagtt tcgggacgtt ctggatctct accggttctt
     8521 ccgccagtag tgcggcaaaa aggataggca gagcagcatt tttagcgccg gaaattgtga
     8581 cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt atccatttag tttgttctca
     8641 gttaacaatt catatccgct accggcgaat cgcccatagc tcaaaagccg ttcagtttgc
     8701 gatcgcgcgc ccactccgca ggggtatacg ctttgatcga cacagcatga atgcggttat
     8761 ccgcaatata ttccatcagc gggccataga ccgtctgctg ttttttaacc cgactcatgc
     8821 cgtcaaacaa ctcacccacg gcaataacct gaaagtggct gccatcgccg gaaacgtgga
     8881 cttcctggag ggagagagcg ttcatcaaca cgctctgaat ttcattattt tccatgggat
     8941 cttcaatcat cagttaataa atcagcgaaa catcttagag caaagttgcg ctggcataaa
     9001 taagcaaaaa gcctcgctga taaatcagac aaggctcgac ttgcaggcag gtttgccgga
     9061 caggcggtta acgccatatc cggcctgaaa aaatttaacg aggcagaaca tcagcaggca
     9121 aattatacaa tttcgccagg gtatacactt tgtcgtttac cccctgaagc gtcacattct
     9181 tgccctgctt tttcgccaga tcgataagat ggagcagcag tgccagtcca cccgtatcca
     9241 cgcgagacac acggctaaga tcgatgctgg taatcccctt caccgcttcc ccacgtattt
     9301 cccaaagcgg tagcagaacg tcctgatcca gctctccgga taacgccagc gtgtcacccg
     9361 tctgcatcca gctcagtgac tcgctcatta ttttttctct tccagagtga ttttctgttg
     9421 agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct ttggtacgca gcagcgttcc
     9481 ccactcgttt tgtttggtgg tgatcatact gacgccttca gcaatcatgt cgtaagcctg
     9541 ccagttgccc gtctgggagt ttttacgcca ctggaagtcc agacgcaccg gcgggcggcc
     9601 attcgggtca ataatggtaa cgcgaatcgg cacaatggtt ttatcaccca gcggctgttc
     9661 tggcgcaatc tgataggttt gaccgtgata catcgccagc gcctgaccgt aagcctgctt
     9721 caggtactca cggaaagcgg caaagtaagc atcacgttga gctggggtcg cactcttgta
     9781 atactggccc agcaccagcg caccggcgta tttcacctgt acgtatggca acagttcctg
     9841 atcaacaatg gtacgcagat aatccgggtt ggcccgaatt tgcggttgct cattcttcag
     9901 gcgatcgaac gttttctgcg ccgcctcgtc catcagctta tacggattgg tctggtctgc
     9961 cgcggttgcc gcactcagcg gtgcaatcac cagcaaagcg accatcatta aacgtttaaa
    10021 catgcgtcgg ttctcctgaa attatttcgt tgtacccaca ggttcagtgg tttcattatt
    10081 acctggcgca gcagctggcg catcgccact attcttattg tcatcgcctt tactaccgta
    10141 aaggaactga ccaatgagat cttccagcac catcgcagac ttggtgtcct gaattgtatc
    10201 gccatccttc aggatagcag ttcccagttc cgggtcttca aaaccgacgt ttaatgccag
    10261 atattgttcc cccagcaggc cggaagtacg aatgctcagc gaactggtat caggaatgtg
    10321 gttataacgt tgttcaattt ccagcgttac gcgcggcaga taggttttcg ggtccagcgt
    10381 aatatccgcc acccggccca caacaacgcc accaatgctg accggagagc gggctttcag
    10441 gccgccaatg ttatcgaacg tcgcataaag cgtataggtc ggttcagtac gtatagacgt
    10501 cacgttcgnn nnnnnnnnca aacaaacagc gccgcaagca gtgctgctaa taaaaagata
    10561 cccacccaaa tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt
    10621 cagcacaaaa tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc
    10681 ccggctaatc ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt
    10741 caccgtgatg gcgaacacca cgctcttaat cagacagttg acgagatcca tacgccagtc
    10801 gacggcattt tgcattgccg accagaagaa cccactatca atgcctttcc aactgacgcc
    10861 gaccagcgat ccgccccaga tccccacggc gacgaagata accgtcaaca gtggtaatga
    10921 aataactcca gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat
    10981 ctccatactg gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttagcgccga
    11041 accagcacgc ccggcaaaca acaacgcggc aaccaccggc cccagttcac gcagtagcga
    11101 taacgccacc agcataccca gactggtttc cgcactataa gtggtcagaa ccagataacc
    11161 ttgcagcccc aacaccattc cgatgaacac gccagaaacc acaataatca gcatcgacag
    11221 gacgccgaca ttatagagct ggcgcaccag cagcggtgca tgtttgcgaa attccggttt
    11281 gccgaccagc gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat
    11341 ccctttatgt ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta
    11401 aaagatcagc gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc
    11461 cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg caacgcctgg gcactgccat
    11521 gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg
    11581 gcacatcgtg agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca
    11641 gcttcaccag cacgcccatg gtaatgggat cttgcccaac aaagggctca tcaaacatga
    11701 tgagatccgg ctccagcgca atcgcacgtg ccagcgctgc acgccgcgcc atcccaccgg
    11761 aaagttcaga aggcataagt ttagccgctc cacgcagccc cacggcctcc agcttcatca
    11821 tcaccgtact atgcaacaat ggcgcgggaa gttgggtatg ttcccgcagt ggataggcga
    11881 cgttgtcaaa tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc
    11941 gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa
    12001 gaatctcacc atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac
    12061 cgatgcccga tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat
    12121 tatcgaagat gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg
    12181 ccacagactg ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca
    12241 atttaacctt gaacccaaca tatttacaga atattacctg ccgtggttag cgaaagctgg
    12301 catttgtttt actttttagc cgcataaagt caaaattaag aatctgttac ggctttctga
    12361 aaatcttcag cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg
    12421 gcactgttaa ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc
    12481 gcgtctattc tttgccgaac ctttggcatc cctccgttga tcatcggcat gacggtggtc
    12541 agtattggta catcgttacc agaaatcatc gtctcgcttg ccgcgtcttt gcacgaacaa
    12601 cgcgatttag ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc
    12661 ggtctggctg cgctggttcg tccttttacc gtccattctg atgttctacg ccgtgaatta
    12721 cccttaatgt tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt
    12781 cgcagcgatg gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa
    12841 cttgcacgtc aggctgaacg tcaggggact gacagcctga ccagagagca gcttgcagag
    12901 ctgccgcgtg aaggtggatt gcccgtcgca tttttatggc tcggcatcgc actgatcatt
    12961 atgccagtgg ccacgcggat ggtagttgat aacgccacgg tgctggcgaa ttactttgcc
    13021 attagcgagt tgacgatggg tctgacggca attgctatcg gaaccagcct gccggaactg
    13081 gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca ttgcggtcgg aaatatcatt
    13141 ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga
    13201 gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt gagcattatt
    13261 tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg ggtattatta
    13321 actggcggat ttatcgtatg gctggcgatg ttgtactggt tatcgccaat actcgttgaa
    13381 taactggaaa cgcattatgt cgcacgtaga gttacaaccg ggttttgact ttcagcaagc
    13441 aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc aatacatcaa
    13501 tcagaatttc acgcttgcct gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat
    13561 ggggatgggg aaatcggggc acatcgggcg caaaatggca gcaacatttg ccagcaccgg
    13621 tacaccttca tttttcgtcc atcctggcga agccgcgcat ggtgatttag gcatggtcac
    13681 cccacaggat gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa tcacggcctt
    13741 aattccagtg cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc gcccggagag
    13801 cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc
    13861 gttagggctg gcaccgacca gcagcacaac cgccacgctg gttatgggcg atgccctcgc
    13921 tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct cacacccagg
    13981 cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata cgggcgatga
    14041 gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctagaag ttacccgcaa
    14101 aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca tctttaccga
    14161 cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa gtattgccga
    14221 tgtgatgacg ccggggggaa tacgcgtgcg cccaggcatt ctggccgttg aggcactgaa
    14281 cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc atttactcgg
    14341 tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca aggataaaca
    14401 acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt
    14461 atggcaaaag cagagaacat tcgtctgctg atcctcgacg ttgatggcgt actgtcagat
    14521 ggcctgattt atatgggcaa taacggcgaa gagctgaaag cgttcaatgt tcgtgacggt
    14581 tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac cgggcgaaag
    14641 gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta tcaggggcag
    14701 tcaaacaaac tgatcgcctt tagcgatctg ctggaaaaac tggcgattgc cccggaaaat
    14761 gtggcttatg tcggcgatga tctcatcgac tggccggtaa tggaaaaagt gggtttaagc
    14821 gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt aacgcgcatt
    14881 gctggcggtc gtggcgcagt gcgagaagtt tgcgacttat tactcctggc gcagggcaaa
    14941 ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt atcattgtgc
    15001 tatcactggc ggttctggtg atgatcggca ttaatatggc cgaaaaagac gataccgccc
    15061 aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac acgctcgtct
    15121 ataacccaga aggggcacta agctatcgat tgattgctca acacgttgaa tattattccg
    15181 atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag gataaaatcc
    15241 cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg ctctatttat
    15301 atggacacgt tgaagtcaat gcactcgtgc cggactctca acttcgcaga atcacgacgg
    15361 ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc gtcacgttat
    15421 acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc agcaagaacg
    15481 ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa actcagcctt
    15541 aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc cgtaaccgga
    15601 gacactgatc agccgatcca cattgaatcg gaccagcaat ctcttgatat gcaaggcaac
    15661 gtggttacct ttaccggcaa tgtcatcgtc acccagggca ccatcaaaat taatgccgac
    15721 aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag aagtgattga cggctacggt
    15781 aaaccggcaa cgttctacca gatgcaggac aacggtaaac ccgttgaagg tcacgcttcc
    15841 cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc ttatctgcag
    15901 caggtcgata gcaacattaa gggcgnnnnn nnnnngggcg ataagatcac ttacctggtg
    15961 aaagagcaga aaatgcaggc tttcagcgac aaaggcaagc gcgtaaccac cgttctggtg
    16021 ccgtcgcagc tgcaggacaa aaacaataaa ggccagaccc cggcacagaa gaagggtaat
    16081 taattcgtta tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc
    16141 gtggtagaag acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca
    16201 aacggtgccg gtaagaccac cactttctac atggttgttg gcattgtgcc gcgcgatgcg
    16261 ggcaacatca ttattgatga tgacgatatc agtctgctgc ctctgcatgc acgcgcgcgc
    16321 cgcggtatcg gctatctgcc gcaggaagcc tccattttcc gtcgcctcag cgtttacgat
    16381 aacctgatgg cggtactgca aattcgtgac gacttgtctg ctgaacaacg tgaagaccgc
    16441 gcgaacgagc tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca
    16501 ctctccgggg gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc gaatccgaaa
    16561 tttattctgc tcgacgaacc gtttgccggg gttgacccga tctcggttat cgacattaaa
    16621 cgcatcattg agcacctgcg cgacagcggc ctgggcgtgc tgatcactga ccacaacgtg
    16681 cgtgaaacac tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc
    16741 cacggcacgc ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa
    16801 gacttcagac tctgataggg tagaagtttg cgacgtttta gcaggagagt acgattctga
    16861 acatgaagca aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagctcc
    16921 aacaggcaat tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg
    16981 cgctggagag caatccgctg cttgagcaaa tcgacactca tgaagaaatc gacacccgcg
    17041 aaacgcaaga cagtgaaaca ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg
    17101 aagagctgcc gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca
    17161 ccagcggtga ctacattgac gacgagctgc cggtctatca gggcgaaacg acacagacct
    17221 tacaggatta cctgatgtgg caggtcgagt tgacaccgtt ttccgacact gaccgcgcta
    17281 ttgctacctc tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag
    17341 atattctcga aagtatgggc gatgaagaga ttgacatcga cgaagttgaa gccgtcctta
    17401 agcggatcca acggtttgat ccggtcggtg tggcggcaaa agatctgcgt gactgcctgc
    17461 tgatccaact ctcccaattc gataagacca cgccatggct ggaagaggcc agactgatca
    17521 ttagcgatca tctcgatcta ttagccaatc acgacttccg cactttaatg cgcgtcacgc
    17581 gtctgaaaga agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc
    17641 ccgggcagtc gatccagact ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta
    17701 agcataacgg tcactggacg gtagaactca acagtgacag cattccgcgt ctgcaaatca
    17761 accagcacta cgcctcgatg tgcaataacg cgcgcaacga tggtgatagc cagtttatcc
    17821 gcagcaatct gcaggatgcc aaatggttga tcaagagtct ggaaagccgt aacgatacgc
    17881 tactgcgcgt gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggcgaag
    17941 aatatatgaa accgatggta ctggctgata tcgcccaggc cgtcgagatg catgaatcga
    18001 cgatatctcg cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga
    18061 aatatttctt ttccagtcac gtcaatactg aaggcggggg tgaagcctct tccacagcga
    18121 ttcgcgcact ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaagcca ttgagcgaca
    18181 gcaagttaac ctctttgctg tcggaacaag gtatcatggt ggctcgccgc actgttgcga
    18241 agtaccgaga gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca
    18301 accgataagg aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag
    18361 gcactgcgcg aatttgttac agccaaattt gccaaacttg agcaatattt tgaccgaatc
    18421 aaccaggtct atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg
    18481 catgtaaacg gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt
    18541 gatggcttaa ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa
    18601 cactaattgt ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca
    18661 tttgtacggt taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa
    18721 attatgacaa ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg
    18781 cgaagccgcg tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggca
    18841 gcgaaacaac ttagcctggc gccccaggtg gtttttgaag ctatcctgac gcgggaaaaa
    18901 atgggcagta ccggtatcgg caatggtatt gccattccgc atggcaaact ggaagaagat
    18961 actctgcgcg ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatc
    19021 gacaaccaac cggtggactt actttttgcc ctgctggtgc cggcagacca aactaaaacg
    19081 cacctgcata ctctgtcgct ggtagcgaaa cgtctggcgg acaaaaccat ctgccgccgt
    19141 ttacgcgcag cccagagcga tgaagagctg tatcaaatca ttacggatac cgaaggtact
    19201 ccggatgaag cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac
    19261 atggtactga tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg
    19321 ctggaagata tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg
    19381 gctcgaactc tggccgatcg agagatttct gccgccgtca gcattgatgt tcgtaatatg
    19441 ccggagtcac cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg
    19501 caactactgt tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc
    19561 cgactgcatc cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc
    19621 gatttgctgg aacctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc
    19681 gttcacgagc tggcagaaat gctgcgtacc cgtctgctgg gtaaacgtga acgtgaactg
    19741 accatggtct ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc
    19801 tttgacgtgc gcttcttgcc gaacccgcac tgggatccga aactgcgccc aatgacaggt
    19861 cttgataaac ctgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac
    19921 cagacgcgaa gctatcttga gctatggtta cctatgctgg aaaccaacaa ccgtagctac
    19981 ctgacggtcg ccattggttg taccggcggg aagcaccgtt cggtgtatat tgcagagcaa
    20041 ctggcagact acttccgctc gcgcggtaaa aacgtccagt cacgccatcg gacgctggaa
    20101 aaacgtaaac catgaccgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg
    20161 cccggcctgc aatgaagctg tttgaattaa tgcagggttt tgacgctgaa gtgctgttac
    20221 gtaatgacga aggcaccgag gctgaagcca acagcgttat tgcgctgctg atgttggatt
    20281 ctgccaaagg acggcagatt gaagttgaag cgaccggtcc acaggaagag gaagcactgg
    20341 ccgccgttat cgccctcttt aactctggtt ttgatgaaga ttaatcttca tcactttgac
    20401 attcaaatcc cttcaagctc ccccggcaat aagaaaagcg cgtactgaaa tcgttatcaa
    20461 tcaacatcaa ttgtacattt cgtgcgcttt ttactttcag aagaacctta agaaaacctt
    20521 aagaagcatt gtttagattt cgtttaagtt aatcgaccat actggagatc gtcagaaaat
    20581 atttccagga gatggcatga ttcgcttatc tgaacaaagt cccctgggca ccggaagaca
    20641 tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt attaagattg tctaccatcg
    20701 tggcgatggt ggcgataaag agatccgccg tgagttaaag tactacgcgc atcttggtcg
    20761 ccggttaaaa gactggagtg gaataccgcg ctatcacggt accgttgaga cggattgcgg
    20821 aaccggttat gtctacgatg tgattgccga tttcgacggc aagccttcca ttacgctgac
    20881 cgaatttgca gaacaatgcc gttacgaaga agacattgca caactgcgcc agttactgaa
    20941 acagctgaag cgttatttgc aggataaccg tatcgtgacg atgtcgctga agccgcagaa
    21001 tatcctctgt caccgcatca gtgagtcaga ggttaccccc gtcgtctgcg acaacattgg
    21061 tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc gtaagcaaga
    21121 gcggttatgg aaacgattta tcgcccaacc ggcgctggct attgccctgc agaaagatct
    21181 gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc gaagcttaat caagctggtg
    21241 ttgttgcata aacggctcac cgcctaactg atacatctgc cgtaaaatcc acgcctgacg
    21301 gctacgcacg tagcccgatg gcgcggagac tttgaaacga agtggattag gtaatacagc
    21361 tgccagtaat gcagcttccg accgggtaag tttgctcgcg ggtttgtgga aataacgttg
    21421 tgccgcagct tcgacgccaa acacaccgtc gccaaattcg gcgatattca ggtaaaccgt
    21481 caggatacgc tttttgctcc agactgtttc tatccccagc gttaatccgg cttccagccc
    21541 ttttcgaacc cagctacgcc catcccataa aaagagattt ttggctgtct gctgagaaat
    21601 cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg cgcgccaggg cttgctcaat
    21661 ggaagcgaca tcaaagcccc agtgctcagg aaatttctga tcttctgcgg caatcaccgc
    21721 cagtcccatc cacggcgaga tttgatccat actgacccag tcagaatgtg ccacgtaacg
    21781 aaaattgcca tgcagccagg cgctgacttg ccgctcgacc attaccgctg agaaaggaac
    21841 aggcgcaacg ctaaacaacg cgatgccccc gccccagaaa atggcgagga caaccattaa
    21901 ccgcagaaga aaacggcgaa cgaaactaaa caccgttaag cggcttttac tcattcagcc
    21961 agaaccagca cgcgggaaac cagcttatca atgccgctcg ccgcttctgc aatgttctgc
    22021 gccagcatat atgccggggt ggtgacaatc ttattgtctt catcaaccac gatatcatcg
    22081 acaggacacg gcacatgctc cgcgcccatc tcttccagca cttctgcggt atcgatatca
    22141 gtaccgatgg tcaaacgtag cgggaaatcg aaaattttcg gcagtatcgc cggtgcaata
    22201 cacataaaac caagcggttt tccggcctga tgcatcgctt gtgccagcgc ctttaattca
    22261 cggtcaacgg tgcattcgct accaagactg gcaaaattgc ttaaattctt cgccgcgcca
    22321 aaaccccccg gcacaatcaa cgcatccagt tcagcggcat cggcctgggc cagaggacga
    22381 atttcaccgc gcgttattcg tgccgcttca atcagcacat tgcgcgtttc cgtcatcgct
    22441 tcaccagtta aatggttgat aacatcaacc tgctgcttat ccggtgcaaa gcagaccgcc
    22501 tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat ttcagaaccg
    22561 tcatagacgc cgcatccgct cagaattacg ccaattttct tcattgtgat cttcctttcg
    22621 caattgactg aaacacatta atttttttaa taaaaatgct acgcatcaca catttaactg
    22681 attcatgtaa caaatcattt aagttttgct atcttaactg cgtgcggcct gaaaaacagt
    22741 gctgtgccct tgtaactcat cataataatt tacggcgcag ccaagatttc cctggtgttg
    22801 gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt ttagtggctt ttgccaccca
    22861 cgctttcagt acttctacgt cgtgacgcca ctcttctttc atctcttcaa tccattcacc
    22921 gacgttatct tcccaggcgg gaaggtcagg agactgaatt tgctgaccca gctgttgcag
    22981 atggcgtaac cccactgacc ccgccgcacc tttaatttta tgcccttcct caacaatgcc
    23041 ttttttatcc tgcgccgtca gattcgactc cagcacgctg acatagcccg gcatcatcct
    23101 ctcaaacacc gctaacccgt cggtgatcag cttcggtcct acaagttcga gatactgttc
    23161 cagcatggga atatcgagca atgcttctga tttactgttc tcttctgtcg tcaccgtact
    23221 ctcctcatca tcctgggtat cccagaattt cttgatcatc gcggttagcg ccggaacaga
    23281 aagcggctta ctcagcacat catccattcc agcattgagg tactcttgct tgtctttcag
    23341 cacgttagcg gttaaggcca ccagcggcgg taaatcctcg cgcggataac gcttcgtcag
    23401 ttcacgagag atatccagcc cggtcatatc tggcaactga atatccagca acaccaggtc
    23461 gtattcgccc ggtttaaaca tctccagcgc cgccttgccg gtcatggcga catcaacgct
    23521 gttacctaat ttttccagca cagaacgcgc gacaatcacg ttcagttcaa tgtcttccac
    23581 cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat catcaacctc
    23641 ttctgctacc gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc cctgttcgct
    23701 ggtaaccgta atatcgccgc ccatattttt cgccagacga cgagaaacgg ccagaccaat
    23761 accggtgccg gtggcaggtt taccgccatg actgtctttc acctggtaat acatggcaaa
    23821 gattttatcc agctcatcct gcggaatgcc gataccagag tcttccactt caaaatgcag
    23881 catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg ccttgctgag tgaatttgac
    23941 ggcgttactg atgaggttcc acaagatctg ccgtaaacgc gtcccgtcgg taatgacctg
    24001 atgcggtaat ggcagcgtcg gctccagatt aaagcgcaat cctttttgtt gcgcctgcaa
    24061 tgcggagaga ttttccagat cggcaaggaa gctggtgaaa tcaaccggtt gattatcaag
    24121 ctggaccttg cgccgttcca tcttatccat gtcgataata tcgttgaaga tattccccag
    24181 cgtgacagcc gaaacatgga tggttttgag atatttttcc tgctcggcgg tgagttcagt
    24241 atccagcaga atgcggctca ggccgacgat accgttcagc ggtgtacgca attcgtgact
    24301 gatggtggag ataaacgtcg ttttgtcgcg gctggcgcgt tcaagcgcat cctgataccg
    24361 cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac ccacgcggtc
    24421 gtagtacggc actttacgga tttcaaagca ggctttgcgt ccgtcgggat aatccagcca
    24481 ctgctcatag gtcagtgaca cattatgacg gaacactttt tcatcggttt caatgacttt
    24541 cgcagcggct tccggtgagt aaacatcagc aggtttcaga tgaaccagtt gtttttcgct
    24601 ttttccggtc agcagctcca tcgcgcggtt acagccggaa aactctttat cttcgttacg
    24661 ataaaaaacc aggtcgggtg aagcgtcaag gaaggaacgt aagaatgagg nnnnnnnnnn
    24721 tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa ttttcagttg gccgaaggtt
    24781 tcctgcagtt ctgcttccgc tttttcacga acggcgattt cctgatttag ctgggcaata
    24841 ttatctttta actgaacgtt gaggctcaaa tcgcgctcac gcatctcctc cagtttttgc
    24901 accagtcgtg acagacgttg tcgtgactcc tccagttgct cgacgaccac cgatagaaag
    24961 tagaccgccc acggcgtaat cagcaaacca aagaagatag aacgaataac atcaatgctt
    25021 tcgacctgac catgcagcac catggttacc gccatttgta ccacaatggc aagaacgacg
    25081 agggccagcg ccagcaacat tgagaagcgc accaaaccta acttcatcat caggtcaaca
    25141 taatactgcg ccagcagacg aatttgcttc attagggaat tccttcacga caacctgccc
    25201 caataatacc caattctgac agttacgtta taaattgtgt gagaaatgcg gaattcccct
    25261 caatggccta cgccgttgga ggtagccacg gacgtcccag cgccgatccc tgtacgccat
    25321 gttcattcag ataacgatcc agcccgacca ttcccgtcca tcgattttcg caccacagcg
    25381 gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg gtggtagatc acttccggtg
    25441 gcgtatggcg aatcatttct ccggcagtga gcgtgtaatc ctccagttca attccgttca
    25501 aacgccccgc ttcccaggct ttcgccataa tgctgccttt cactatatgc agcggatgca
    25561 gctttatgcc gtccacgccg gtttcaacca ctcgctccag cgtttgcaaa cattcagcct
    25621 gcccttcgcc aggcaggcca acaattaagt gagaacacac cttcagccca cgctcacgcg
    25681 ccagctgcgt cgtgcgctga tagcaggcaa aatcatgacc gcgattgatg cgatgcagtg
    25741 ttttgtcgtg ggcggtttgt agccccagct ccagccacac ttcgtagccc tggtccttat
    25801 attcgcaaag cagatccagc accgcatccg gcacgcagtc cgggcgggta ccaacacaca
    25861 aaccgacaat attggcctgg ctcaccgcct gctgatacat cgaacgcagc acctgaactt
    25921 ccgcaaaggt gctggtatac gcctgaaagt aggccagata gcgtttagcg cggttaacta
    25981 aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg ctgctgcgct tcatcggcaa
    26041 acgaggcaac attacagaat gtgcagccgc cacgcccgat ggtaccgtca cggttagggc
    26101 agctaaaacc gccgtgcagc gtcagcttat gcaccttttg cccataacga cgggtgagat
    26161 caccaccaaa catattgact aatttctgta actgcataat ctgatagacc gcgccttgaa
    26221 aagaggccaa agcctgccat ttttagccca attcggcgat gacctggatc aatcgtcccg
    26281 gcctgctttt atcaaatgca taataaatca aaattaccga aatttcatgc acaatcacat
    26341 aaatcacttt tgcttatctt gtgtcagatt tttttatctc cttatggatt ttaggcaaaa
    26401 acagtagcat gaaacgttat taccaattaa ggcagtataa aatgctggct ttctcgtcaa
    26461 ttcaaggcag gataagggtt aacacgcctt tatgacagtc aggaactggc tgtttctcta
    26521 acgacgcccc ttttagcctt aaatagaaaa tccaatttaa tttcagataa ttaataaaga
    26581 attttgcgct aaagcacatt tctgtaccaa taagcttgcc atttgacctg tatcagcttt
    26641 cccgataagt tggaaatccg ctggaagctt tctggatgag cagcctgctc atcatattta
    26701 tgcagtaatt gagatcccct cttcaccgta ttaaccgatg cgaaaaggac aacaaggggg
    26761 cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc acgctcttac tgtgcccgtg
    26821 cgcagcggtt cggaagtggg gttcccgcag agcctggggg aggttcacga tatgttgtac
    26881 gataaatccc ttgagaggga taactgtggt ttcggcctga tcgcccacat agaaggcgaa
    26941 cctagccaca aggtagtgcg ta
//
LOCUS       DAWDRC010000004        22314 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1272_length_22314_cov_2.673615, whole genome shotgun sequence.
ACCESSION   DAWDRC010000004 DAWDRC010000000
VERSION     DAWDRC010000004.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 22314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1272_length_22314_cov_2.673615"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10214..10223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13796..13805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18446..18455
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20796..20805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5460 a         6064 c         5479 g         5271 t
ORIGIN      
        1 tcttttttgt tattaaaatt cacattttta acacttagta tcaactgaaa cagttagcgt
       61 ggtattaatt agttcaataa ttagtgtata cttgattttg tgatatgggt cacgaaacaa
      121 aggcccagct aaaagattat gtcgaggtaa aaatcatgaa aatcaaaacc actgttgctg
      181 cattaagtgt gctttctgtt ctctctttcg gtgcattcgc tgccgactcc attgatgctg
      241 cacaagcaca aaatcgtgaa gcaatcggga ccgtatccgt aagtggtgtg gcgtcttcgc
      301 caatggatat gcgtgaaatg ctgaacaaaa aagcggaaga gaaaggcgca acggcctacc
      361 agattactga agctcgtagt ggtgacacct ggcacgctac ggctgaactg tacaaataaa
      421 ccctcatcgt cttgtccgac gatattgccc ccggttcggg ggcttttttt gcgctaatga
      481 cgaacattaa aacgcaaatg cccttccagc tcttcctctg cctcatcaaa cagcaatatt
      541 aacgcgccaa aacggcgacg cgttttctct cccagatgaa caaattcaat ctcaagtggt
      601 agcggcagga catcgttcat taacacatcc catagtgagt cgagatcgcg taccttatct
      661 ttcgccagac caaaggtttg gctaaagtca cgataaaaat cctcctgact ctcaatctca
      721 tcaaaatcaa aggtataaat attcatctgt tgccaccgtc acgtttcgcg gcgagttctg
      781 ccgttcatcg ctaagtatag ccatgaaaaa accgacgctt cgggcgccgg ttttgactta
      841 actatcggtc aacgcatgtt gatacttatg gagcataccc gtaagccgat gtactggttc
      901 cgtcacctgc ggtggcgctt gccagatgcg tagcttttcc tggtagattt ccagttcttc
      961 cagcaactgg ccaaagtacc gacgacgttt atcatcgcta cgggcagata tcacatggtc
     1021 tgctgtgcgg cgcatttgcc ggtgaaacgc cgataaatcc tcgttaaccg gaatcggcgc
     1081 atcacgcagg cgctggtgcg cgataatcat cgtcagcgcc aggcgaaatt tcggcaaatc
     1141 ccccgggaac ttattcatca gcaaaaacag ctgctgataa agtgccggga ggtgattctc
     1201 tttacgacgt gccacattgg tagtcatcgc ggaaacagcg gcagaaacaa actgattaag
     1261 cagtacacgt ccagtcctgt cgcgcgattt atcccgcacc agcaaaataa cggtgaacgc
     1321 gagcacacag ccgacgattt gccctaatgc gctgtcgaga aactgactga aatggaaagt
     1381 catcgggtta tccagcacga taatgtttat ggtgctggcc aacgccccca tcgagcccaa
     1441 tctccgtttc tgtacttcta taccgaggaa gaatcccagc actgccaggc taatacacag
     1501 cagcaacatg ctctgttggg tattagggat aatcaccaaa aagtagagca gccctaacgg
     1561 tagcgctgcc agcgtcccgt agataaagtc gatcgccacc atgcgtggat tcggcaaacg
     1621 cattgccagt gacgtcacta ccgcaatcat caccattgca ccactgccgg aagtccagcc
     1681 cgtccacagc cagaaaagcg tgcccagaat gcaagaaagt gtggttcgcc agaagttaac
     1741 cattgcatga tgacgttcgg ctgactctac ttttacttcc ggttcgcctt gcaggatctc
     1801 ttcttcggtg gcgttgattt ttgtgttact gataacgccg cgcttgagaa gctgataacg
     1861 cgttgccgcc gcgacccagc tataaatggt gacaggcgtt tcccgttccc cggtccaggc
     1921 gatcactctc cgcaggcgtt tgagctgctt gtggacgtcc tgcgcagttt ctaccggcgt
     1981 gtcaaaaaat tcgcggaaag tatcagtgat caattccggg cgcgtattct gaataagata
     2041 agtttcgcag gattgggtaa tcagcgtcag cgatagcgta ttgatcgctt ttaaacgtcg
     2101 attggcccgc gcccagcggg aagattccat attcaggttg ctgcgcatac cttgtaacgc
     2161 cgtagtacgt cgcaccagat cgccccaggc tttatcgaca acttcaccat cgccatgctt
     2221 gatacagagt tgcattaatt gatattgcgc aaccagcaaa ctttccagct ctcgatccac
     2281 ttcttgtttg atcgatcgcg gagaaaagag caaatccgcc ataattgcac acacaatacc
     2341 gataacgatc tcgctacaac gttcgacggc aaactgcggc gtaagcaatg gttccggctg
     2401 aatggtgatc acaatgatca gcgcggtata accggccagc ccccacgcat acgagttttc
     2461 tattcgtacc agcgaggata tccaggtaca aaaaccggcc cagatacagc acaccagaat
     2521 catcaataat ggtgctcgga tcatcgcaat gatgattacc agtccggcaa tacagccaat
     2581 aaatgtgccg atgatgcgca aaaagccacg atagcgaata gcgccagaat acggttcacc
     2641 tcccgcagca aaggccggac cggcggcaac aatcgccgct gtcagtaccg cccagcgtgg
     2701 cgtttccagc tggaagtgaa agccaacaaa cagcgccagt acaatggcgg tcgccagttt
     2761 taccgcaaag cgaatatgtt gattagcaat ggagaaaata cccatcgtga ttaaccaaac
     2821 tcacgcaggc gatgagccat tttacggaag aacgaatcct gactttcgtc gcgatcttgt
     2881 ttgccagtga ctaccaccgt agcagtggtg cccgcaggcc agatgttctc ttgctggttg
     2941 tcgagacgaa tacgtaccgg aacacgttgc gccaaacgta cccattcaag gttagagtcg
     3001 atggtcgcca tccctttgtc gtcacgcgtg ctgctggcat tggtgacccc tgcggcaaca
     3061 ctatcaacag tccctttcag cactttgtta ctgccaagcg gcgtgatctc cgcacgatac
     3121 ccagggcgca ccccttccag cttagtttct tccatatagg ccagtacata gaaggagttc
     3181 tgtttcacca gcgcaaccgc cgttgatcct cgagtaataa actcaccggt atagacgttg
     3241 aggttggtca cccagccatc tgctggcgcg cggatgactg tacgttcaag atccagtttt
     3301 gccagatcgc gggtcgcctg cgctttcgct aactgatgca gaactgtttg tagtacgttg
     3361 ttggcctggt cgatctcttc gcgagacatc gcctgcacac cgagacggtt acgacgtccg
     3421 gcctcctggc gtttctcctg cgccagtacc tgataataag caacatcggc ttgcgcttcc
     3481 tcaagcgcct tttgataacg cggctggtcg atggtgaaca gtacctgtcc ttttttcacc
     3541 agctggttat catgaacatt cacctgggta atgagtccag aaacgtccgg cgcgatcgca
     3601 acgacgtcag cgctaaagcg cgcgtcacgc gtccacgggg attcggtgta atagacccag
     3661 gcattaaaaa ttgcgatgaa ggccagaatg actaatacga ccgtgatggc cgtacgggag
     3721 aattttctta ttagtgtttt cacttcaacc tcaaacgaac agtcgcgata tcaaataaaa
     3781 caagcagcaa tagagcgcgg tgttgaacaa cgccggatgc cagacaaagt cgtaaatacc
     3841 tgttggcaca agtacccggc gcaccagcca gaaaatcgcc agtgataaaa gcaattcgaa
     3901 aaatatcggt gggaaggaca gcccaaacac cacgataacg ggaaacagac tcatgttgac
     3961 cttggttgta tagagagagc aggcgttatt attttcagca tctgtcgccg cagagaaggg
     4021 catggaaagc cgggcgagag caacattgct gtagattgat atttaatata ttaacgtaac
     4081 tgttatgctg ttatctatat tatgtgatct aaatcacttt taagtcagag tgaataatgg
     4141 aacgactaaa acgcatgtcg gtgtttgcca aagtagttga atttggctct tttaccgccg
     4201 ccgccagaca gctacagatg agcgtttcgt ccatcagtca gacggtgtca aaactggaag
     4261 atgagttaca ggtaaagctg ttaaaccgta gtacacgcag cattggcctg actgaagccg
     4321 gcagaattta ctaccagggc tgccgtcgga tgctccatga agtgcaggat gttcatgagc
     4381 aactgtatgc cttcaataac acccccatcg ggacgctgcg cattggctgt tcttcaacta
     4441 tggcacaaaa tgttctcgcc gggctgacgg caaaaatgct gaaagaatac ccaggtttga
     4501 gtgtcaatct ggttaccgga attcctgccc ccgacctgat tgccgacggt ctggatgtgg
     4561 tgatccgcgt cggcgcgttg caagattcca gcctgttttc ccgccgtctg ggcgcgatgc
     4621 caatggtggt gtgcgccgca aaaagctatc tcacacaata cggcatacca gaaaaacccg
     4681 ccgatttaag tagtcattca tggcttgaat acagcgtgcg acccgacaat gaatttgaac
     4741 tgatcgcacc ggaagggatc tcgactcgcc tgatcccgca agggagattt gtgactaatg
     4801 atccgatgac gctggtgcgc tggctgactg caggcgctgg gatcgcctac gtgccgttga
     4861 tgtgggtgat caacgagatc aatcgcgggg aactggagat cctgctgcca cgttaccagt
     4921 cagacccacg cccggtttat gcgttatata ccgaaaaaga taagctgccg ctgaaggtac
     4981 aagtcgtgat caactcgcta acggattatt ttgttgaggt cggtaaattg tttcaggaga
     5041 tgcacgggcg cgggaaagag aagtaattaa tattgtactg ttacgttgta caaacctgtg
     5101 ccaacgggtt cccctcaccc taaccccaga ggggcgaggg gaccgttcgt acacggagaa
     5161 ggattaatta tccttctgaa aatactgaaa ttattacgca gtaccgccaa cagtcaggtt
     5221 atcaactttc agcgttggct ggcccacgcc aaccggcaaa ctttgccctt ctttaccgca
     5281 gacacccacg ccgttatcca gtttcaggtc gttgccaacc atcgaaatct gctgcatggt
     5341 ttcgataccg gaaccaatca acgttgcgcc tttcaccggc ttagttactt taccgttttc
     5401 aataagatac gcttctgagg tggagaaaac gaatttgccg gaggtgatat ccacctggcc
     5461 gccaccaaag ttcggtgcat agataccgta ctcaacggat tcaataattt cctgcggggt
     5521 cgatttaccc ggcagcatat aggtgttggt catacgcggc atgggcagat gagcgtagga
     5581 ttcacggcga ccgttgccag tcggcgtcat ccccatcaaa cgcgcgttga gtttatcctg
     5641 catgtagcct ttcagaatgc cgttctcaat cagcacgttg tactggcctg gcgtaccttc
     5701 gtcatcaatc gccaccgaac cacggcgatc gaccatcgtg ccatcatcaa ccacggtaca
     5761 cagttctgaa gccaccaact ccccgacctg tccactaaat actgaagtgc cacggcggtt
     5821 gaagtcgcct tccagaccgt gacccaccgc ttcatgcaac agcacgcccg gccaacctgc
     5881 gccaagtact accggcatgg tgcccgctgg tgcagcaacg gcagaaagat tgaccagcgc
     5941 catacgcact gcttcttttg cccacgcatc cgcacggact tcgccatcga gatcggcaag
     6001 gaagaactca taaccaaaac gaccgccgcc accactggca ccgcgttcgc gtttgccatc
     6061 ttcttcgacc aaaacgctca cggaaagacg caccagcgga cggacatccg ccgctagtgt
     6121 gccgtcggtg gccgcaacca gaattaattc atagacacca ctgaggctgg cagtcacttc
     6181 ctgtacgcgc ttgtccgctt cgcgggcaac cttatcgacg cgacgcagga tatccaactt
     6241 ctcttcacgg ctcatgcttt gcagcggatc taccgaggta tataacgggc tatgctctac
     6301 agcgcccagc gtctgtactt tgccatcacc actatcacgg acgatggtgc gcgccgcttg
     6361 tgcactctgt tccagcgcca gcaggctgat ttggtcagca taagcaaatc cggttttttc
     6421 accactgatt gcacgcacgc caacgccctg atcgatgttg taagaaccat ctttaataat
     6481 acggtcttct aaaacccagg attcgtgata gctcgactga aaatagagat cgccataatc
     6541 aaggcgacgt tcggccagtt gaccgaggat cgcgaacaag tcctgatgtt tcaggccgtt
     6601 cgccgctagc aattgttcac ttaccaggtt cagactcatc gtttttgcta ctcgttagtt
     6661 actgcagtag aggatttttt acggctgccg gatgcggcgt gaacgccctg tccggcctac
     6721 ggttatgttc tggctcgttg gcctttggca acgattatca tatgagattg gggcaattac
     6781 gcgccctcgt caaatcattg cgctttttct ttacgcggtt gtcgcaacac ttcgttgatt
     6841 tgcggatcgt ccagcggacc cgaaatgtga tagcgcaaaa tggagacttt gctccacagc
     6901 ggacccagca ctttactggc ggcaaacact gccgcgccaa caatggggtt aaccgcaaag
     6961 gccgcagcca cacccaccgt cgcagaaatc tctggtgcga caaccgcttc catattcagg
     7021 tcgcgacgta ccagatttac cgaccctttc atggcgatgt ccgcttccag gccatccacc
     7081 agcgtgtcgt cggtgtgcat aacgccgtct ttaatccacg cggtgctgcg aatggagtca
     7141 aaatagaacc cttcgccaaa agtgtctctg aaatcaaaac gcagcttacg catcagggca
     7201 tctacgctca ataagcgcag caattgcccg gcatgtccgg tattgatttc ggtaatttcg
     7261 cctttaccca gttgagtatg aatgatgcca ttcaacgtcg cctcatctgg ctgccagggt
     7321 gctttgcgcc agtgtaaatc gtaatccaca ttaaatgacg actggcgtat gggcgtcgtg
     7381 acaccaaaaa attctgcggc ggcatcaatt ttctgcccgc gcagttttcc tttcagcgag
     7441 gtacgttcat tccccggatt gttaacccat tcaccatcgg cagtgagccg cgaaaaacca
     7501 ttatcaatca gtccattagt cagcgttaac gtatcgccag aaatggtgat atcactgtca
     7561 atacgaccga atttttgccc ccagaaccag cactctgcgc atcgtatttg tgcgtctggc
     7621 cagccacgga agttaatgcg ttccgttgtc gggaaaggtg atgactgtgt tgaatcacca
     7681 cgagttttcg ccacgcttgg gttgtaataa agatatttga tattcgccag ccacggtgcg
     7741 ttattacgca tcgccagcgt ggcgttgatt tcacgccctt gcgcctcaac cagggtgcca
     7801 tttgccgtcg gttgcgaaac aatactcagg ttattccatt gctgatttcc cagcgacaac
     7861 ataggcgtac gtaacgttat gtgttgtggg aaactcgctg caccaccgac actctccgcc
     7921 gcgcctttct ggaacagggc cagccactcg gcaccattca tcggcggcat attgagttca
     7981 acgccacttt gttccggcaa cggcgggagc gttttactgt ctgccgccca aatagcacga
     8041 tcgagcgtca gcttttgacc gagcaaccag cggctattga aatagttatc cgcaccagcc
     8101 tgtccggtta attcaaagct gttgagattg ccatcaacct taacgtttac cggtagtggt
     8161 tcaccagcag gtttggctaa cggtgaaggt aagtgactgc tcacattctt caaatcgccg
     8221 tttagctcta cgttataggt cgcgccagca tgataaggca gatcaatgcc cactttacca
     8281 tcccacgcca cgctgccact taatgcttcg ttcaccgctt caggcagaac gccggttttc
     8341 gctggttgcc agttaccgtt gaggtttacc gctacctggt aggcttttgc cccttctttg
     8401 gtggaaaaat ccacgttcaa cggctgatta aaccagcttg ctgtcagtgg ttcactttgc
     8461 agatcgccat tgataaagct gaatttaccg ctcaaatttt tcagggtgct gtcgagtggt
     8521 ttgataaaca gactgttatt acgcagcgtc acttcacctt tcgcggttac caactcgccg
     8581 ttcagcggga tatcaagatg taagcgagca ttcacatcgc cgtcgagctg gagttcttgc
     8641 agggtcgcac ccagggaatc tttcagcggt gtctcatcaa agtaagggcc aacggcttta
     8701 cccggacctt taatgtcagc gtcaatcagc agtttttctt ttgagtagtc agggatcact
     8761 gcggtgagat tactcgcgcg cacgccgccc agattaacgc catcggtttt catccataaa
     8821 ccgtcgttaa taaagtccag ttcaatatca aggttagtta atgcaggcca gtccggctgg
     8881 aaggcaaact tcgcgttgcg cagcggcacc agcacttcag actgaccttc gttgtgttta
     8941 taggggaaga gttgcggatt gccaccataa accagcgtcg cgttatccgc ttcaccgccc
     9001 tgaatcgcgc cacttaagta atcaaccagg tctttaccca tcaagttttc cgggaaatag
     9061 cgccaggctt gtgaaccatc atcggtactg atgccagcca gaatacccag ccagggttca
     9121 tcgttagcag gttgcaggta acgaaaaccg ccgcgcgcat ggacggcttt ggctttaacg
     9181 tcaatattac gcccatccag ctggaaacct ttgtcattgt tcagccagct tatcgttgcc
     9241 tggccgtcgg cgatttccag tggcgcacgg aataccgttt cgtaaggcat ctttgcctgc
     9301 ttcatcgacg cagtaagcaa accattttct acgctgccgg aaagcgtccc ggagaagtgt
     9361 tccgcacccg gtaataattt ccattgcttc caggccagat cgctccacga tgcctgaaaa
     9421 cgggtcttgt ctgccgcctg aagcgggata tccagcgcca gagtgttaat cttgccgctc
     9481 ggttgtgtgg agcgccaaac atcacccagt gcaggtgaaa gtttcgcgac cagcgggcgt
     9541 acgccctcca ggcctgccag ctccagatta ctggcgcgaa tccggagttc gtcactgcgt
     9601 ttattgtctt tgccgccaac gtcctgttcc ggtatccagg ccagcgtcaa tgctccgctc
     9661 ggccagggtt tgccgtccat cgtgatccgt gtatcgggaa tagagaactg ccatcccgga
     9721 ttttcacgcg taatatgcgc agtcagatta tccaccgaca gcgtatgcgt ttccttctcg
     9781 cccaaccagc tggcaccgcc ctgtttcagc cagacgtcac cgccggttac atcgcctttg
     9841 tcgatcgtca tccagccttc aagggagaac tgtgccgttt ccagcgcaat attgtcctgc
     9901 atccatttac cgagccacgg cttcaggtcg atgtcatccg cctggagcca gacgcgacca
     9961 ttgcttaaca acccctcatc atcgcgcaaa tccatacgca cctgcatcac gccgtgctgt
    10021 ccggtaaggc tggagagact taccaggcct tccgcacggt gtcgacgtgg atcgttcagc
    10081 caggtgagct gtgggatcgc cagctcggcg cgctgaccgg atggcgtcag gaaactgact
    10141 tcactgtcgc ggagatcgaa atggtcaaat tgacgaagaa acagatcgct gatgtgactg
    10201 gcttccaggc tatnnnnnnn nnnaaggcta gcattaccac agccgctggt gataggagtg
    10261 ttggtgcgaa agcgcagctg ccagaaagtg aggtcgcgaa actgccagcg catatgtaac
    10321 aggctctgcc agacatccag cgcaagagta acgcgtttaa ccgaaaattc gccgccatct
    10381 tttagttctg cacggatgtc gtgcgcttca agcgtcggac caaaattctg ccagctggct
    10441 gagagctgac tggcttctac cggcatgcca gtcgcggatt ctattttgtt gaggatttcc
    10501 ggacgccagg cgtcaagatg cggtaaagca atacgcaggc cgctaaccag cagggcagcg
    10561 atcacaacga gcgcggctcc agtaagcagt aaaatccccg gcaatcgcct cacccgtcac
    10621 tccttgtctg ctaaaaatgt gactcaaaaa ccctttgccg gatggcggcc cagcatctgt
    10681 ttacatcatt acgacgtcaa actgctcctg gttatagagc ggttcaattt gtactttaac
    10741 ctgtttgcca acgaaaattt ccacttccgc cagcgagtgt gactcttcgc ctttcaaggc
    10801 ttcggctact gccggagaag catagaccag gaaacggtcg gagtcgtaag catggtggac
    10861 acgaacaatc ccgcgcatga tttcatagca taccgtttcc acggttttca ccgttccgcg
    10921 accgtggcag gttgggcatt cgttacacag tacgtgctca atgctttcgc gggtgcgttt
    10981 acgcgtcatc tccaccagcc ccagcgccga aaaaccatta acgctggttt tcacccgatc
    11041 tttgctcagc gcctgctcca gcgagtgcag cacgcggcga cggtgatctt cattattcat
    11101 atcgatgaaa tcaataatga taatcccgcc cagattgcgc aaccgtaact ggcgagcgat
    11161 agcctgcgtc gcttcaatat tggtattgaa aatggtgtcg tccagattgc gatgaccgac
    11221 aaacgccccg gtattgatgt ccacggtggt catcgcttcg gtctggtcga taatcagata
    11281 accaccggat ttcagttcta ctttgcgttc cagcgctcgc tgaatttcgt tttcgacatc
    11341 aaagagatcg aaaatcggct ggcgtccggt gtaatgctcc agcttgctgg tcatctcggg
    11401 aatgtactcc gaggtaaact ccagcaatgc ttcgtaagtc aggcgtgagt caacgcgaat
    11461 gcggtcgagt tcggcgtcgg cgaaatcacg cagaacacgc tgcgccagcg ccagttctcc
    11521 gtacagctga taacgggtct gcgggcgttt tttacgctcc ataactttgg tccagacgcg
    11581 tttcagataa gcggcatcgg aggccagttc agcctcgcca accccttccg ctgcagtacg
    11641 gatgataaac ccgccctgct cgtcgcaata ctctgcgacc acttttttca ggcgttcacg
    11701 ttctgattcg ctttcaattc gttgggatac cccaacgtga gaagcccctg gcataaacac
    11761 cagatagcga gaaggcagcg tgatatcggt ggtcaagcgc gcacctttag tgccaagcgg
    11821 atctttcacc acctgcacca tcagatcttg cccctgacgg accagttccg agatgtcgcg
    11881 caccgtgaat tgcttttgtt cttcacccgc cacacattca gtgtgcggca tgatgtcaga
    11941 tgcatgaaga aacgcggctt tatccagccc aatatctaca aaagccgcct gcatacccgg
    12001 aagtacacga cttacacgac ccttgtagat attgcctact attccgcgtc gcgcctcacg
    12061 ttcaatatga atttcctgca gaataccgcc atcaatatac gccactcgag tttccgaagg
    12121 cgttacgttt accaacaatt cagccgtcat gtttatccct tttctcacgc agtgcgttaa
    12181 aattacttaa taattcatac gtttcaacca gcggtaagcc gactacggcg tgaaagctgc
    12241 catttatctt cctgacaaaa cagccaccca gcccctgaat accgtatgca cctgctttat
    12301 ctaacggttc gccgctggcg acatagcccg cgatgtcttc gtctgttaac gttctgaaag
    12361 tcacatcggt gaccaccagg caatcgagaa tgtgctggct gtcagccaac gccactgccg
    12421 tcatcacctg atgggtctga cccgataatt tgcgcaacat ctgcgccgca tgctctgcgt
    12481 cgcgcggttt ctcgagcact tctccgttca ggataacgat agtatccgca cccagcaccg
    12541 ggagatcctg cgccgtttgc gcgacacccg cctgtgcttt ctcgcgcgcc agacgcacaa
    12601 catactgctg cgcgctctcc tgcggctgac gctgctcctc aatgcccgta acaatacgtt
    12661 caaaggtcac gccaagttgc gcaagtaact cctgacgacg cggagaaccg gaagctaaat
    12721 acagagaagt catagaaacc tttattgcac tgcaaactgc tgacggactt tacgcatcag
    12781 caagaaaatc cacggccaga gaacgccatt gactacacta ctccagaaca cttccggtct
    12841 gaaagagacg ttaatcacta aaaactctgc ccagaaaaca ataatatcca ccaccagcga
    12901 aagcaacatg acgaccagcg cctgctgcca taatgcgagg ttgcggaaaa gctggtattt
    12961 cagcgccacc aggtaagcaa tgatgctcat cgccaatacg cgtacgccaa gcgtcgagcc
    13021 gctgatcaga tccagtatgg cacccatcac aaaacctgtg cccacattta cgcgatgggg
    13081 caaggccagg atccaataca acaagatgag taacacccag tttggccgga aaacaatcag
    13141 gttatccggc cagggcatga tctgcagcaa cagcgcaatg aggaaagaga gccagattac
    13201 ccagcgtccc tggctacgat agctcgccac tattgtcctc ccggcgcacg cgcaggcggt
    13261 tgagcaccac tttgcggcgg cgtagccctt tgtggagagc gattagcagc gggctgtgtg
    13321 ggcgcagcag gcgcagtaac tgcatttcct gtcgccggtt gctgcggagt cggctgagcg
    13381 atccccgttg ccggttcggg taactttggc cccatcgcgt ctggcgaagg caatacctgc
    13441 ggcatcatct gcatcagacg ttcattagca acacgatgca cctcttccgg cgtcatcggg
    13501 ttagcgccgt tacgatccgc gccccacagc agcagcagat aacgcaaacg ttgcagtccc
    13561 gcagtcggac gcgcctgaat cacagtataa gcgcgctggg tatcgagttt tacggaagag
    13621 acaaccgcca ccggatagcc ttccgggaaa cgaccgccca gaccggaagt caccagcaca
    13681 tcaccaacac gaatatccgt attcgctggc agatgctcaa gctgcaaatc atccgtacaa
    13741 ccgttaccgg ctgcaatcac gcggatatcg ttgcgcagca cctggattgg cagcgnnnnn
    13801 nnnnngcgtg ggtcgcatca caaatcagca gcacgcggct ggtcagttta gcgacggcca
    13861 ccacctgacc aacaacacct ttgtcgctga taaccggctg gccttcataa acgccattaa
    13921 cgctaccttt atcgataaca acttgatcgc tataaggatc gttaaccgtg gagataacct
    13981 gagtcaccat tttctgctca tcctgacgca gcggggaacc cagtagctcg cgcagacgcg
    14041 cgttctcctg tttgtattgt ccaagcatca gcagttcact gtttttcagc aacagttcct
    14101 gacgtaacgc ccggttttca agttctagtt ggtcacgcga ggccagcgtc tgcgatacgc
    14161 catccagcaa ttcacgagga gcattggaaa caaagtagaa aggactgacg gcggtatcca
    14221 tataagtacg gctttgactg aacgtcccca ggcggctgtc ggcaataata atgccgagcg
    14281 ccaccagcac cgccagaata aggcgaatct gtagcgacgg gccacggcta aaaattggct
    14341 tcataagtta tgcgtattct cgtatcagac caggcagggt aaacagacac ttcccctgcc
    14401 tgcatccgat tactcttcgc tgaacaggtc gccgccgtgc atgtcgatca tttccagcgc
    14461 tttgccgcca ccgcgcgcca cacaggtcag cgggtcttca gcaacaacga ctggaatgcc
    14521 ggtttcttcc attaacaaac ggtcaaggtt acgcagcagt gcgccaccac cggtgagcac
    14581 catgccgcgc tcggagatat cggaagccag ttccggcggg cactgttcca gtgcaaccat
    14641 taccgcgctc acaataccgg tcagcggttc ctgcagtgct tcgaggattt cgttggagtt
    14701 cagggtaaaa ccgcgtggaa caccttctgc aaggttacgg ccacgaactt cgatttcacg
    14761 tacttcatcg cccgggtaag ccgaaccgat ttcgtgcttg atacgttctg cggtggcttc
    14821 accgatcaga gaaccgtaat tacgacgtac atagttgatg atagcttcgt cgaaacggtc
    14881 accaccaatg cgcacggaag aggagtaaac cacgccgttc aaggagataa cagcaacttc
    14941 agtagtacca ccaccgatat caaccaccat agaaccggtc gcttcagaaa ccggcaggcc
    15001 agcaccaatt gcggcagcca tcggttcttc aatcaggaag acttcacggg caccagcgcc
    15061 ctgcgcggat tcacgaattg cgcggcgttc aacctgggtc gcgccaaccg gcacacaaac
    15121 cagaacgcgc gggcttggac gcataaagct gttgctgtgc acttgtttga tgaagtgctg
    15181 gagcattttt tcagtcacga agaagtcggc gataacgccg tctttcattg ggcgaatggc
    15241 agcaatattg cccggcgtac ggcccagcat ctgcttcgcg tcatgaccta ctgcagctac
    15301 gcttttcggt gaaccggcac gatcctgacg aatggccacc acggaaggct cattcaatac
    15361 gatgccttgt ccttttacat aaatgagggt attcgcagta cccaggtcaa tggacaagtc
    15421 attggaaaac atgccacgaa attttttcaa catactaagg gataatcctg aaagctgggg
    15481 cggaaaagaa aatccgctta ctttaccaac cacacgcagc agcgacaagg cgcaaaaatc
    15541 atctgctacg gtgaaaatta gtgcagttcg tttcctttgt tacaaatctc tgcctgagtc
    15601 cagaaaggct taatgcatca gcagcattcc tcgcctgttt gcaaccgcgt aaggtcattc
    15661 atctgcatat gtgctgcaac aatctggcga gcagacaagc acactcccat gagacgcagc
    15721 gcgcgttatt ctacgtgaaa acggattaaa cggcaggtta aaccgagtat ctttgtgaat
    15781 attttttcac gttagtatca agtggctgtg aggacgcgaa aaaatcccct tgcccacctg
    15841 taacaccgcg ctgaatcagg gtctgccact cgcttcgcga acgcacgccg gtggcgtaaa
    15901 cctgggtgct ggtcccggag caggcttcca ccaggctttg aaccagcagt tggttctccg
    15961 ttcgcttctc aatgtttctg accagccccg gatggagctt gagtaactca acattaagtt
    16021 ctttgatcca actggtgctt accagcgtca aaccagcctg gttgacggct acccgtaccc
    16081 ctaaagcatt cactaaacga ataacaggtt gtaagcgact gatatgttga ccaacatcgg
    16141 cctctgcaag ttcaataatt atgtgtcttc gttgcgattt ttcacattgc attaacgtat
    16201 cgcgcagcca acgctgaaaa cgcgggcgaa tcagcgactc aacggtaacc tgaatcgcca
    16261 gattttcctc tggccagtaa cgcaatagtg gaataagacg gctgatttgc aggcggtcat
    16321 actcttccga taaaccaaac tgcaagacca tcggcatata ctctgccgag ctaacctctt
    16381 cattaccatc gaaaatgcgg cacatgagtt cacgatgatg aacccggcct tcgcgagtaa
    16441 ccgccggttt ttgataaagg cgcgggccgc cgcgactgag catttgctcg ataagcgtac
    16501 gccagcgaac attaccgcgt cctttttcag gcaacgagtc atcgtaaata gcccagctat
    16561 tgccgccctg caatcccgca ttacgcgtgg cagactctgc atgttccatt acctgctcgg
    16621 tatcctgacc actacgccag gcacagatac caatgtggag catatcgtcg cgatcgagca
    16681 ttttattgtt cggcaaggta tcgacggctt tgattaactg actggcgatg ctctctgcct
    16741 cttttaacgt ccggtgcggt aacagcgcag caaaatcact gcggtggtaa cgcgccagca
    16801 gtgcgccagg gtagcgcatc ataaatgtcg atagcagatt cgtcagagag aagaactgtt
    16861 cttcaacctg gctgtgcccc caggtatcac tcaacatatt gaaatccggc agacgaatca
    16921 tcatcacgat cccgtgggta cctactttct cttgatcttc cagtaacgtt gctaactgat
    16981 tatcgaaaaa gagtcggtta ttgaggccgg ttttcatgtc ctgggcggca taagagcgga
    17041 tcagcgtatc aagacggctg tgttgttcgc gtgcgttctg aatttcacaa agcagcgtat
    17101 ccagcgcact gctggttctg ggcggccatt catagatggt tcccaacaca ttagagccac
    17161 gctcaccgtt taagatacga gtagcccggg tttccagcaa ttcttgcccg gcaagttgcc
    17221 gttgtaacca gcgtaccgcc aggaagagca taagaatgat aaagccaatc gcccccgtga
    17281 gcggcgcggt agtcattaaa gaatggaaat agttgcccat cggatcctga taaaccagac
    17341 gcagcgacat ccccggatgc tttatcaacg gaacgctcag ttcacgaaac agatcgttgg
    17401 tgccaaccgg acgataacta ccatttctgg ccagggtata aacctgctta tcaccatgga
    17461 gcaggtctac acgaacgata tctgccgaca tcattaattc ggtaatttgt ggccttaatg
    17521 tgctgaagtc attcgacaca aggtaggtat caatcgccgt cgccaccgcc tgaacacgat
    17581 gactaaactt atactgaatg gcgttgtaga aacttagcga acagcccagc aaagtcacaa
    17641 aaattgttaa cccggtgagc agcgtgacaa aggccgaaaa tttcgtcgtt aatctcatcc
    17701 ttgtgttaac tccgatagtg aggaagcggg catactagca aatcagattt atctcgcaat
    17761 ttattgcgct tcatcggctt tgcttttcca ttagcgagta tagtcttcag aaattatttt
    17821 ccaatccatc atgcacatga ggaccactta tgcaggcgtt acttttagaa cagcaggacg
    17881 gcaaaactct cgcatcagta cagactctgg acgaaagtcg cctgccggaa ggcgatgtca
    17941 cggttgatgt tcactggtcg agcctgaact acaaagatgc gctggcgatt accggtaagg
    18001 gaaaaatcat ccgtaatttt ccgatgattc ctgggattga ttttgccgga actgtacgca
    18061 ccagcgaaga tccgcgtttt catgccggtc aggaggtgtt actcactggc tggggcgttg
    18121 gtgaaaacca ctggggtggg ctggcggaac aggcgcgagt gaaaggtgac tggctggttg
    18181 ccatgccgca agggctggac gcgcgtaaag caatgattat cggtactgcc ggttttaccg
    18241 ccatgctgtg tgtgatggcg ctggaagatg ccggtgttcg cccgcaggat ggggagattg
    18301 tcgtgacggg tgccagtggt ggcgtcggca gtaccgccgt ggcgctgctg cataagttgg
    18361 gttatcaggt cgttgctgtt tccggtcgcg aaagtaccca tgaatatctg aaaagtttag
    18421 gtgccagccg tattcttcct ggcgannnnn nnnnntgggg caattgacac cgttggcgac
    18481 aaagtgctgg caaaagtgct ggcgcaaatg aattacggcg gctgcgtggc ggcctgtggt
    18541 ctggcgggtg gttttactct gccaaccacg gtcatgccat ttattctgcg taatgtccgt
    18601 ttgcaagggg tggattcagt aatgacgcca ccagcacgcc gcgcacaagc ctggcagcga
    18661 ctggtcgccg atttaccgga atcattctat acccaggcgg caaaagagat atctctggca
    18721 gaggcaccga agtttgccga ggccatcatt aataaccaga tccagggtcg cacgctggtg
    18781 aaggttaact aaccatttag caaggattaa taagagaggg aactcatttt gaaatcatta
    18841 ttcaatcggt taacggaaaa agcggttagt cggacagctt tcgtcgaaca ccttggtcag
    18901 gaagttgtac aacatcatcc aaactggaaa gtcatgattt cgactgacca taaattgatg
    18961 cgcattgata ctccaccaaa cagccattat tgatacgcct ccgtcgcctg ttaggtttat
    19021 ggtgctttgc cttggcgacg ctacgcttaa tctgttactg atttctggta ccatgggggg
    19081 aataatctga ttttatttga ttacaaatta accacttaaa cctattgaat gcggaacatt
    19141 gtcagtcggt taattttgcg tgctttagca ttcacatctt tccggacgat gcagtgaaaa
    19201 ttgggtaatc ccccgcaacc gctgcgtaat gtcgtctatc ttgtcgcgat cctggcatcc
    19261 ctacattatt tgtggtctgt gaagattatc tcattgcagc ccctcatctt cgcagggctg
    19321 gctttacagc ttttcacctt acgttataag aagttccgtc gatgatggcg ctaatttcgt
    19381 gaattgtgcg gcttgttgca aattacacgg tgttgaaggt tatttacatg ttagctgttg
    19441 attatcttcc ctgataagac cagtatttag ctgccaattg ctacgaaatc gttataatgt
    19501 gcgacctcgt cctccctgac gcagtttttg cgctgcggaa aaggtgacat tggcgcaacg
    19561 aaggtatatt ttgttttttg ccggaggata gcagcagatc gctgcacaat gtccgtcaag
    19621 tctaacattg acactctggg gcaaaataga ccggcgtccc ggcctgctgg aatttatcgc
    19681 tatgcataca gctgtcgggg catacgcttt acagacggcg gtgaaacgcc tgtcacaatc
    19741 acactaaaca aagagtacgg aacccactca tggatattcg taagattaaa aaactgatcg
    19801 agctggttga agaatcaggc atctccgaac tggaaatttc tgaaggcgaa gagtcagtac
    19861 gcattagccg tgcagctcct gccgcaagtt tccctgtgat gcaacaagct tacgctgcac
    19921 ctatgatgca gcaaccagct caatctaacg cagccgctcc ggcgaccgtt ccttccatgg
    19981 aagcgccagc agcagcggaa atcagtggtc acatcgtacg ttccccgatg gttggtactt
    20041 tctaccgcac cccaagcccg gacgcaaaag cgttcatcga agtgggtcag aaagtcaacg
    20101 tgggcgatac cctgtgcatc gttgaagcca tgaaaatgat gaaccagatc gaagcggaca
    20161 aatccggtac cgtgaaagca attctggtcg aaagtggaca accggtagaa tttgacgagc
    20221 cgctggtcgt catcgagtaa cgaggcgaac atgctggata aaattgttat tgccaaccgc
    20281 ggcgagattg cattgcgtat tcttcgtgcc tgtaaagaac tgggcatcaa gactgtcgct
    20341 gtgcactcca gtgcggatcg cgatctaaaa cacgtattac tggcagatga aacggtctgt
    20401 atcggccctg ctccgtcagt aaaaagttat ctgaacatcc cggcaatcat cagcgccgct
    20461 gaaatcaccg gcgcagtagc aatccatccg ggttacggct tcctctccga gaacgccaat
    20521 tttgccgagc aggttgaacg ctccggcttt atcttcatcg gcccgaaagc agaaaccatt
    20581 cgcctgatgg gcgacaaagt atccgcaatc gcggcgatga aaaaagcagg cgttccttgc
    20641 gtaccgggtt ctgacggccc gctgggcgac gatatggata aaaaccgcgc cattgctaaa
    20701 cgcattggtt atccggtgat tatcaaagcc tccggcggcg gcggcggtcg cggtatgcgc
    20761 gtagtgcgcg gcgacgctga actggcacaa tccatnnnnn nnnnntctcc atgacccgtg
    20821 cggaagcgaa agcagctttc agcaacgata tggtttacat ggagaaatac ctggaaaatc
    20881 ctcgccacgt cgagattcag gtactggctg acggtcaggg caacgctatc tatctggcgg
    20941 aacgtgactg ctccatgcag cgccgccacc agaaagtggt cgaagaagca ccggcaccgg
    21001 gcattacccc ggaactgcgt cgctacatcg gcgaacgttg cgctaaagcg tgtgttgata
    21061 tcggctatcg cggtgcaggt actttcgagt tcctgttcga aaacggcgag ttctatttcc
    21121 tcgaaatgaa cacccgtatt caggtagaac acccggttac tgaaatgatc accggcgttg
    21181 acctgatcaa agaacagctg cgtatcgctg ccggtcaacc gctttcgatc aagcaagaag
    21241 aagttcacgt tcgcggccat gcggtggaat gccgtatcaa cgccgaagat ccgaacacct
    21301 tcctgccaag tccgggcaaa atcacccgtt tccacgcacc tggtggtttt ggcgtacgtt
    21361 gggaatctca tatctacgcg ggctacaccg taccgccgta ctatgactca atgatcggta
    21421 agctgatttg ctacggtgaa aaccgtgacg tggcgattgc tcgcatgaag aatgcgctgc
    21481 aggagctgat catcgacggt atcaaaacca acgttgatct gcagatccgc atcatgaatg
    21541 acgagaactt ccagcatggt ggcactaaca tccactatct ggagaaaaaa ctcggtcttc
    21601 aggaaaaata atttgtgacg cgcggtgttt ccatcgcgct tagcaaagaa cgtaatcaac
    21661 gccgggcctg ttaatgctac ccggcgtttt tattgccgtt aactaacggt agcctgcaat
    21721 ttccgctttc aacgcgctaa tttcagcatc gccatcaaca attcccagct caaagcagta
    21781 tttctttgtt tcccctggct caataaaggg cagacgcccc tgcgctcgtt ctttatcacg
    21841 accgtcagga aaactattac tgacctccag tcccataaca tattcacccg cgcctaaatt
    21901 tttccactgg ataaagttgg gtagctgcat ggtgtcgaat ttctcgtaaa ttccccaccc
    21961 cagttcagcg ttcaccagcg caactttact catcccctga ctgtcgctat tcaacgtgag
    22021 atagtaaacc tgctcgtcaa aaccgggctg tggggtacaa atctccagat gactggttaa
    22081 cccttccgcc gcatgcgggg ttcgtggcgt tacgctttta gccgacagca agatttctgc
    22141 ctgtggcgat aataaaggcc agccgtaatt aaagtgataa agaatttgca gcggctggcg
    22201 ggtgaaacca tgattagtca ccttgtcttc aatgcgcagc gcattttcgc cataacggca
    22261 gcgaatggtc ctttccagcg tcagattttc accgaacaat cgtgtttccc gtac
//
LOCUS       DAWDRC010000005        20710 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1403_length_20710_cov_2.477754, whole genome shotgun sequence.
ACCESSION   DAWDRC010000005 DAWDRC010000000
VERSION     DAWDRC010000005.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_20710_cov_2.477754"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    477..486
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1685..1694
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3534..3543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7425..7434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11143..11152
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11769..11778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12686..12695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13627..13636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14566..14575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16422..16431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16801..16810
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    20287..20296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5150 a         5651 c         5234 g         4555 t
ORIGIN      
        1 atgcgttgct gatggtcaaa cagtgccact atcgcttttt cgctggtgta attgcctttg
       61 gcttgcagat cgaggcgctc aaaacggaag gtgtatcctg ccagcgtcac ctgctgaccc
      121 ggctgtaaat tcaggctgat ctcctgacgg ctgacgctgg aaaccacaat ccccgcggca
      181 aataacagca cgcccgcatg cgccagcagc gccggaagct gagcacgttt gccagagaca
      241 aacgtcgcca gcacaatcac caccagcatc aacagagcaa acggtaacgt cgcgcggtta
      301 aaatacggcg cgccaacgga gaggcgtccc cagcccagca ggccgtaaat catcggataa
      361 agcgttccca ccagcacgat cagtagcact gcgcaaaaca gcaacagcgt agcgaggatt
      421 aacatttccc gcgataaccc cgaaaaacgc accgccgggc caccgtcccg cgctcgnnnn
      481 nnnnnngcca gagacgcaag gctaatcagt gcaaacaggc tgaacaacgg cacggcgcgg
      541 acgttatcca gcgcgaacgc atgaaccgaa accagaatgc cagaacggac aattaaggtg
      601 cccagcagcg acagcatcag agttactatc gccagcaaca gcgaccagtg gcggaaaatc
      661 ccccgctggc gtgtcaggga taaactgtgc agcagcgctg tggcagaaag ccagggtaat
      721 aacgacgcgt tttccaccgg atcccagaac caccagccgc cccagcccag ttcgcaatag
      781 gcccaccagg aaccgaggat gatccccgct gttaatgcgc tccagccagg tagtgcccaa
      841 cgccagcaaa ttcgggcgca ggtaccatca aactcgccgc gcagtaaact cgccagcgcc
      901 acgctcgccg ccaccatcaa accgccgtag ccgagataaa gcagcggtgg atgaaagata
      961 agaccgggat gttgcagcat cggattgaga tcgcggcctt cgattgctgg tggaaatatc
     1021 cgcacgaagg gatcggacca cagcaccaca aacagtagca gtgtggcgag catgagagat
     1081 aaaacggcta gcgtcagcgg aaatagcgga tcggtttgct gtcgataatg ccaggcaaac
     1141 agcgcgctcc aggcggaaag cagcagcacc caaagcagca gcgaaccttc atgaccgccc
     1201 cacaccgccg ccagtttgag ttcccacgac agcaggctgt aactatgttg ggcgacataa
     1261 atgaccgaga aatcgctgat gagaaaacaa tacgtcagta cgccaaaggc gagcagcagg
     1321 agcgcgaact gcgccagaat gccgatgcaa gtgagtcgca tcatggcagg ccagcgcaac
     1381 cgcactcccg cgaaggccgt caacggggtc aacacgttga ccccgagact taacaacaac
     1441 gccagaaaac cgacttcagg aaggaatacg tccagaaacc acctctgttc tggcttacgc
     1501 caccgttaac tgcccggcgt agagaatgaa aaagcgcagc atcaacacgc cgaccaggct
     1561 ggccccacat gccgccagca cggcaggaat gccggaactg cgattgaccc acggtttgag
     1621 caacattggc acaatcagcc ccagcccggc gacaccaagc cagaaccacc ccgtccagaa
     1681 aacgnnnnnn nnnnaaccgc cgcccaatgc cgccaccagc gcacgcactt taccgtcatc
     1741 gcccagcgcc agaccgacaa aaaacgccac cagcaggaag atttcacccc ataccacggg
     1801 gatttccata cggtgtacaa agtgcgcttc cgtggaatgc gggttactgc gttggcgtat
     1861 cgccatggcg atcagcgcca ccgccgcacc agacgagatg ccggagaaga ggaacagcac
     1921 cggcaggatc gggttattga ggaacggata cgatttcagc gccgacagca gaaagccggt
     1981 ataagccccc aacaacaccg ccagcaccag catcaatgtt tccagtccgc gatgaacggg
     2041 cgtcagcaga ctcaagacct tttgcacgat ccccagcttc ggcaaccagc gttgttgcag
     2101 ggcaagcaaa tcatgttcaa agattttcgc cagccacagc accagcacca ccatgtaaag
     2161 ctgaaacagc atcaccccca tcgacatcac cgaggtaaaa ctgtagtgga acatcagctt
     2221 ccagaaggtc cacggtcttg tcaggtggaa gacgaggatc aacagaccga ggatcaccgc
     2281 gccaggcccg acaatcagcg tggtgcgcag caacgtactg tctgcaccgc ctgcctgagg
     2341 gtagaagcga cgtaacagca cggccagcgt caccagaccg gcagaaatac caatcaaaaa
     2401 caggtagatg gcaatcggcc agtcccacac cagcgattca aaatgaaatg cggaagtctg
     2461 cgtcattggc tcacctcccc gtatttaaac ggtacgcggt acagcttcgg tttggttccc
     2521 agcgccagct tgtagcggta agtgggcttc tggcgcagca gttgcgaaat ctcactgctg
     2581 ggatcgtcca gattgccaaa cgtcagcgcc ttcgtcgggc aagcctcaac gcacgcgggc
     2641 agcttaccgg cctgcaaatg ggtcttacgg cagaaatcgc atttgtccgc cgtcttcgtg
     2701 accggatgga taaagcgcac gcggtacgga caggcagcga tgcagtactg acaaccgaca
     2761 cagagatccg gattaacatc gacaatccca ctggcagcgt cgcgaaaaga cgcaccggtc
     2821 gggcagacgt caacgcacgg cgcatgatcg cagtgctggc aagacttacg gaagaagcga
     2881 tatttcacat caggaaattc gccctgcggc tcgctacgaa taatcgtcag gcgcgagacg
     2941 ccttccggca ccttgttcac ttcccgacaa gcatccatac aggcggtaca gccgatgcat
     3001 aacgactcgt catgcaccat gccgtaacgc accccattga tattcaacgt tttcgccacc
     3061 acgcgtcctg cggtgccgct gacggcggcc agcacgccga cgccggtgag aaactggcga
     3121 cgagaccagg tcatggctgc tccttaagca acggaacaga cgccgggtta aagttcggat
     3181 tggtgcgctg atcgctgtgg caatcgacgc aaatcttaat ccgtcctttg tcacttaacg
     3241 tctgcatcgt atcttgctgt ggatgcaggg aatggcagct ggcgcacgcc actttggtta
     3301 cgtggacatc gtgcggccag aacgcttttt gcaactgttc cggcagatgg caggacatac
     3361 agacgctgtt ctgctctcca accttgtaca tcggctcgtt aaagcgcatc acatccttca
     3421 ccccttcgcg gtgttgcggt gatggctgac cgtggcagtt ggtgcaggtg accggcagtt
     3481 tgttattcgg gttgataaca gaagcatgtt tgccatgcat accttcggta tctnnnnnnn
     3541 nnnagtccag acaggcggca tccgggttac gctgctgggt aacttcgtag cgatcatcag
     3601 atgcctgcgc cgcaggggta gcggtaattc cgttcaggct ccacaacagg cccgacgcca
     3661 gcaccccggc agttaataac gaacgtaata cgctcatatt cactccattg tgttttccgc
     3721 cccttgcgag gcggaacggg gttattggct taacagaccg tttttacgtg cctgctcttc
     3781 ccactgcggg atcaccgttt tgatgaagtc ctgcttctcg gccttgattt gctccatgtt
     3841 caggccaatg gcctgctggg ctttctcttt ggtcgagata tccgggatct ggatttcatg
     3901 ggttatgcct ttggtcgcca gcaggcgcgc cagtttggtg cgtgcatccg ccgctttatc
     3961 catcgccgta ccgagcatcc tcagaccttc ttccggtgcg tgcatatgaa tgccgtggga
     4021 agcgatcgcc aaatcccaac gccactgggc atgacggata tcgtcctgaa tcggcttcat
     4081 ttcggcttcc gttgcgcctg catccagcgc cgctttcgct tcgaagtgag cgtgaaccag
     4141 ttgatcttca accttgattt tcaggtcgtt aatcgactgc ttacgttccg cgaccacttt
     4201 ttgcaaggca gctttgtcct gggtatggca gttcgcacaa gtctgggcga agttatcaaa
     4261 cggattacca attttatggt cggtgtagag tttgccttcg gcgttctgca cttttggcat
     4321 atggcaatcg atacaggtca cgttgttttt gccgtgaatg cccgctgtcc aggtttcata
     4381 ttccgggtgc tgcgctttca gcattggcgt tttcgacagg gagttagtcc agtcagagaa
     4441 ggcaattttg tcgtaatact gctccatatt ttcgactttc atgccgtcat cccacgggaa
     4501 tttaaccgct ttgtttttgc cgtcgaagta atactccaca tggcactgac cgcaaaccat
     4561 cgattgctgg tcgaaacgtc cggctttctc aaaaggttta ccaatggctt ccatcgcgcg
     4621 agccgcatac ggacgggaaa gggttaactc cggtttgcct ttggcgaact ctggtgaggc
     4681 ggtgttatgg caatcggcac aacctaagtt gttgacgatt tccgggccgc cgcgcgccca
     4741 ttttccgtgg aagtagccat cttcgccgtc tttctggatc agacgcgcca catccgggct
     4801 tttacaactc cagcacgcca tcggtagcgg accatcttca gcgtttttcg gcgcaccggt
     4861 acgcagggtt tcacgcacat cggtcacagc aaaggcatgt ccacgcggct tgttgtaatc
     4921 gcgcgagaag ggataccctg cccacaggat caccagccgt ggatcttccg ccagggcgtc
     4981 aacacgctct gactgttccg aggtagcttt ccaggagaga tattgatcgg gatgctgcgg
     5041 ggcaaaggtt tcattcttcg cttccacagt tacaggtgtt gcgggagcag ccgtttgttc
     5101 agcgtgaaca gaagtgaaaa agaaaaaagg aatcaataag ctgaagatac ggcgtgcgtt
     5161 tatttttatc cttgtcatag ggcttcatcc gaattgcgcc attgttgcaa tggcggtttt
     5221 tattgttttt cacgacagta accgcaccta cactgtcatg acattgctcg tccctatgtg
     5281 taacaaataa ccacactatg aatgttgtct ttaatcaatt gtaagtgcat gtaaaatacc
     5341 actttagagt tagtcagtat cttcctcttt ttcaacagca tgcataactg catgttcctc
     5401 aaagaattaa tcaacttttg ttgctgacct tcaaaaatta ccctgccgtt tatttgcaca
     5461 attctacttt tgcgtgatct gtcgcccaaa tactaaacaa aactgccaat acccctacat
     5521 ttaacgctta tgccacatat tattaacatc ttacaaggag aacaaaagca tgagtcaaat
     5581 tcacaaacac accattcctg ccaacatcgc agaccgttgc ctgataaacc ctcagcagta
     5641 cgaggcgatg tatcaacaat ctattaacgc accagatacc ttctggggcg aacagggaaa
     5701 aattctcgac tggatcacac cgtaccagaa ggtgaaaaac acctcctttg cccccggtaa
     5761 tgtgtccatt aaatggtacg aggacggcac gctgaatctg gcggcaaact gccttgaccg
     5821 ccatctgcaa gaaaacggcg atcgtaccgc catcatctgg gaaggcgacg acgccagcca
     5881 gagcaaacat attagctata aagagctgca ccgcgacgtc tgccgcttcg ccaataccct
     5941 gctcgaactg ggcattaaaa aaggtgacgt agttgcgatt tatatgccga tggtaccgga
     6001 agccgcggtc gcgatgctgg cctgtgcccg cattggcgcg gtacattcgg tgattttcgg
     6061 cggcttctcg ccggaagccg ttgccggacg cattatcgac tccagttcac gactggtgat
     6121 cacttccgac gaaggtgtgc gtgccgggcg tagtattccg ctgaagaaaa acgttgatga
     6181 cgcgctgaaa aacccgaacg tcaccagcgt agagcatgtg gtggtactga agcgtactgg
     6241 tggaaaaatt gactggcagg aagggcgcga cctgtggtgg cacgacctgg ttgagcaagc
     6301 cagcgctcag caccaggcgg aagagatgaa cgccgaagat ccgctgttta ttctctatac
     6361 ctccggttct accggtaagc caaaaggcgt gctgcacact accggcggtt atctggtgta
     6421 cgcggcgctg acctttaaat atgtctttga ttatcatccg ggcgatatct actggtgcac
     6481 cgccgatgtg ggctgggtta ccggacacag ttatttgctg tatggcccgc tggcctgcgg
     6541 cgcgaccacg ctgatgtttg aaggcgtacc gaactggccg acgcctgccc gtatggcgca
     6601 ggtggtggac aagcatcagg tcaatattct ctataccgcg cccacggcga ttcgcgcgct
     6661 gatggcggaa ggcgataaag cgatcgaagg caccgaccgt tcgtcgctgc gcattctcgg
     6721 ttccgtgggc gagccaatta acccggaagc gtgggagtgg tactggaaga agatcggcaa
     6781 cgagaaatgc ccagtggtcg acacctggtg gcagaccgaa actggcggtt tcatgatcac
     6841 gccactgcct ggcgctaccg agctgaaagc cggttcggca acacgtccgt tcttcggcgt
     6901 gcaaccggcg ctggtcgata acgaaggtaa cccgctggag ggggccaccg aaggcagtct
     6961 ggtgatcacc gactcctggc cgggtcaggc gcgtacgctg tttggcgatc acgaacgttt
     7021 tgagcagacc tacttctcca ccttcaaaaa tatgtatttc agcggcgacg gcgcgcgtcg
     7081 cgatgaagat ggctattact ggatcaccgg gcgcgtggac gacgtgctga acgtctccgg
     7141 tcaccgcctg gggacggcag agattgagtc ggcgctggtg gcgcatccga aaattgccga
     7201 agccgctgtg gtcggtattc cgcacaatat taaaggtcag gcgatctacg cctacgtcac
     7261 gcttaatcac ggggaggaac cgtcaccaga actgtacgca gaagtccgca actgggtgcg
     7321 taaagagatt ggcccgctgg cgacgccaga cgtgctgcac tggaccgact ccctgcctaa
     7381 aacccgctcc ggcaaaatta tgcgccgtat tctgcgcaaa attgnnnnnn nnnngatacc
     7441 tcgacgcttg ccgatcctgg cgtagtcgag aagctgcttg aagagaagca ggccatcgcg
     7501 atgccatcgt aacccacaat tgccggatgc gctccgggca acaagtcgga aacgatttgt
     7561 aggcctgata agacgcgaca gcgtcgcatc aggcattgat tgccggatgc ggcgtataac
     7621 gccttatccg gcctacattc ggcaacggct gtaggcctga taagacgcga cagcgtcgca
     7681 tcaggcgttg attgccggat gcggcgtgaa tgccttatcc ggcctacatt cggcaagggt
     7741 tacccaagcg ttaaccttct cccataaggg agcgggaatt aaaacaatcc ctacattacc
     7801 tctggagaat ctgtgatgaa tggtacgatt tatcagcgga tagaagacaa tgcgcatttc
     7861 agggagttag tcgaaaaacg gcaacggttt gccaccatcc tgtcgattat tatgctggca
     7921 gtttatatcg gctttatttt actgatcgcc ttcgcgcccg gctggctggg caccccgctg
     7981 aatccgaaca ccagcgtcac acgcggtatt ccagtcggtg tcggggtaat tgtgatctcc
     8041 tttgttctca ctggtatcta catctggcgg gcgaacggcg aattcgaccg tcttaataac
     8101 aaagtcctgc atgaggtaca agcatcatga aaagagttct gacggcgctt gccgccacac
     8161 tccctttcgc agctaacgcc gcggatgcta ttagcggggc cgtagagcgc cagccaacga
     8221 actggcaggc gattattatg ttcctgattt tcgtcgtgtt tacgctcggc attacctact
     8281 gggcgtcaaa acgcgtacgt tcgcgtaatg actactacac cgcaggcggc aatatcaccg
     8341 gttttcagaa cgggctggcg attgccgggg actatatgtc cgccgcctca ttcttgggga
     8401 tctccgcgct ggtgtttacc tccggctatg acggcttaat ttactcgcta ggcttcctgg
     8461 taggctggcc gatcattttg ttcctgattg ccgaacgtct gcgtaacctc ggtcgctaca
     8521 cctttgccga tgtggcctct taccgtctga aacaggggcc gattcgtatt ctttcggcct
     8581 gtggttctct ggtggtggtg gcgctttacc ttatcgccca gatggtgggc gcaggtaaac
     8641 tgatcgagct gctgtttggc cttaactatc acattgcggt agtgctggtc ggcgtgctga
     8701 tgatgatgta cgtcctgttc ggcggcatgc tggcgaccac ctgggtacag attatcaaag
     8761 ccgtgctgtt gctgttcggt gccagcttta tggcctttat ggtgatgaaa cacgtcggct
     8821 ttagcttcaa caatctgttc agcgaagcga tggcggtaca cccgaaaggt gtcgacatca
     8881 tgaagccggg cgggctggtg aaagatccta tctccgcgct ctctttgggg ctaggactga
     8941 tgtttggtac ggcgggcttg ccgcatattc tgatgcgctt ctttacggtc agcgatgccc
     9001 gcgaagcacg taagagcgtg ttctacgcca ccggatttat gggctacttc tacatcctga
     9061 cctttattat cggctttggc gcgatcatgc tggtcggtgc gaatccggaa tataaagacg
     9121 cggcgggcca tctgattggt ggtaacaaca tggcagcagt tcacctggcc aatgcggtgg
     9181 gcggcaacct gttcctcggc tttatttcag cggttgcttt cgccaccatt cttgcggtgg
     9241 ttgcgggtct gacgctggcg ggcgcatcgg cggtgtctca cgacctgtac gctaacgtct
     9301 tcaaaaaagg tgcgaccgaa cgtgaagagc tgcgggtatc gaaaatcacc gtactgatcc
     9361 tcggtgtgat tgccattatc ctcggcgtgc tgtttgagaa tcagaacatc gcctttatgg
     9421 tggggctggc gtttgccatc gcggcgagct gtaacttccc gatcattctg ctttccatgt
     9481 actggtcgaa actgactacc cgtggcgcaa tgctgggcgg ctggttagga ctgattactg
     9541 cggtggtgct gatgatcctc ggtccgacga tttgggtaca gatcctcggt cacgaaaaag
     9601 ccatcttccc gtatgaatac ccggcgctgt tctctatcag cgtggcattc ctcggtatct
     9661 ggttcttctc ggcaacggat aattcagcgg aaggtgcgcg cgagcgtgaa ctgttccgtg
     9721 cgcagtttat ccgctcccag actggctttg gcgttgagca aggccgcgcg cattaatctc
     9781 tccccttccc cggtcgcttg atcggggaat ttcttcagaa catcatcctg gccaccagcg
     9841 gcgaaaccaa taccatcaat acacctgaaa gcatcatgac caggcttgca actacgcctt
     9901 cttgttgacc cagttcatac gaccgcgcag tcccggcacc atgagatgcc gcgccgaatc
     9961 cagcaccttt cgccatccct tcgcgaatag agagacgtaa aaacaacacg tcgccaatcg
    10021 ccatgccaaa cacaccggtg accaccacaa acagcgccac cagatcaggc tgtccgccga
    10081 gcgactggct cgctgccaaa gcaaatggcg tagtgaccga acgcaccgcc aggctacgct
    10141 ggatctcatc tggcagggta aacaatcgcg ccagccacac tgagctggtc actgccacca
    10201 ccgttgccgt cagtacgcct gccgtcaacg acatccagtg acgcttaatg atcgcccgat
    10261 tgtcatacac tggtaccgca aaggcgatgg tggccggacc taatagccat agtaaccagt
    10321 gcgcttcgcc aatatagttt tgccaggaaa tattgccaaa aaccagcagc ataaccagta
    10381 gcgtaggcgt cagcaccaga ggcatcaatg gcaatttacg aaagcggcga taaaggcgtt
    10441 tgttaagaaa atagataact agcgtaattg ccagacagag catacttagc tgaaaattag
    10501 ccacggttta acctgcgcat ttcatagcga tagacttttt ccactaccca ggcagtcgtg
    10561 cccagcacca ttaccgtact cagcgcgatc acagcaaaaa tacgccagcc atccaccagt
    10621 aatagttgtg catagttcac cacagccacc acggcaggga caaaaaacag caacatttcg
    10681 gccaatagcc aacgcgcccc ggctcgaacc cattgcagag gaagaatgcg acaaacaatc
    10741 agcgccagca tcaggatcat ccccaccagg ttggcaggca gcggcaaatg caaccaggag
    10801 acgagatact gcgcaaaaat aaataaacct gcatacagca caacctgaaa aaatacctgg
    10861 atagattgca ctacggcagg ggtaatgcgg cgaagcgcca cgggcatgag aggatatcct
    10921 gaaaataaaa cagaatggta agtatagaga gcgctcgccc ctgactgaaa tgaattaaaa
    10981 tcattgcagg tatagtcaaa gggaatagtt atggatatca gaacgctgcg ttattttgtc
    11041 gaagtcgtgc gccagcaaag cttcacccgc gcagcggaga agttgtttgt cacccaacca
    11101 actatcagca agatgctgaa gaatctcgaa gatgagctga acnnnnnnnn nngatccgcg
    11161 atggtcgtcg gctgcttctc actgatactg gtcgcgtcgt atttgagcgc gggctggctt
    11221 tactggctga gtttcggcaa ctggaagctg agcttagcga tatcaatcag cttaaaaccg
    11281 gcgttttgcg gctgggaatc ccgccgatgg ttgggatgtt gatggccggg ccgattggcc
    11341 tttttcgcca gcgttacccc ggcgtagaat tgaaaatttc ggagtttggt ggtctgacgg
    11401 tacaacaagc ggtgatgaac ggtgaactgg atatggcact gacagccctg ccagtagaag
    11461 aaagcggcct gacaacgtta tcgctgttta gtcatccact atgtgtactg acgccacatt
    11521 caggtaaatg gcttgaatgt gatgccgtct caccggagac attggcggaa catcctctac
    11581 ttatctataa cgaagatttt gccttaagta agcaattgat gaagttattc gatgaacata
    11641 gcgttaagcc gagaattgca gttcgcagcg ggcaatggga ttttctcgcc gcgatggttc
    11701 aggccggtgt cggaatagcc atattgccgg aacccatttg ccagcgatta gataaaaaca
    11761 ccttaaaann nnnnnnnnta ggaatgattt ggcgtgaggg cgtttacctt tcccactctg
    11821 cccacgcgtg gctgagctgt tgtgaaggat actggttaac gccagagtaa gtcgggttgg
    11881 cgggctcgcc cgccaaccat gcagattact gattttcctc aatcagcaac gcttcaagca
    11941 aatcgagatc gtgcagtaat ttttgcagcg tttcgttact gatctcccgc gtggcgcgca
    12001 ggtggtacag ttcagcacgt tcagaacgca atgccgccag acggaagcga cgctccaggt
    12061 tctcttccag gattgaactt tctacatcat tacgtccatc ggcgcgacga cgcaggttac
    12121 caatgacgcg agaactaacc tctgtgagca gctggttatc gatattctct tcggtatccg
    12181 ccgccaaacg ctcctccatt ttctggatcg ccacaatcgc cacttctgcc gttgccgctc
    12241 gtgcaatacg ttcctctttc aattgttgcg aatggtccgc gacttctatg tgttgtagca
    12301 gaataggcaa catcaccacg ccgacaaaca atgagaagag aatgacgcca gccgccagga
    12361 acaccaactc atagcgcgcc gggaagacgt taccatccgg caagagaagc gggatagaga
    12421 gcacaccggc cagagtgatc gccccacgca ccccggcgaa agacgcgatc aggatttctc
    12481 gtgtagtcca cgaaccaaac tccatcggct tcttcttcag gaagcggttg ctgaactttt
    12541 tcatcgtcca cagccagccg aaacggacca gcatcagtgc cgcatatatc agaataatat
    12601 cggtaaacag catccagatt tcgacgttag ggtcgatttc tgccgccatc agcgacgtct
    12661 ccagaatccc cggcaactgc agaccnnnnn nnnnngcgaa cggcaaatca caccggagcg
    12721 ggtgatggtc atcccggcgg caaccgcagc gaggatgccg gaaacgccaa tatgttccgc
    12781 aatcagataa gaagcgaatg gcagtaagaa cagcagaacg atctgcgtcg cgggttcatc
    12841 accgccccag cggctgagga atcgcagcga gcgaccatac agccagctca ccacaaaacc
    12901 ggcgagaata ccgcctattg ccactttcat aaattcgacc gtcgcaccgc cgacggtgaa
    12961 gatcattgtc cccatcgcca ctgccacggc aaacttcaac gacaccaggc cggaggcgtc
    13021 attcatcaac gcttcgccct gcaaaatgcc catgattttt ttcgggatgc gcccctcgcc
    13081 aacaatcccg gagagcgcta cagcatcggt cggagaaagc accgcagcca gcgcaaaggc
    13141 ggggatcagc ggaatgcccg gcaccaccca gtaaataagg aagccaatgc cgactacggt
    13201 gaccaccacc agcgccagcg cgaggccgaa aatctctcgc ccatgttcga gaaattcacg
    13261 ggtcggcgtt ttccagccat cagcgaacag caacggcggg ataaacaaga ctaaaaagag
    13321 ttcaggatca aactccacat gcaaaccaaa cgtcggccac gccagtagcg caccgatggc
    13381 gatttgcata agcggaagcg ggatctgaaa gggcatgaca cgagtgacca ccccggacag
    13441 cgagaccacg agggtcatta tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc
    13501 gatgtttttt atgatctgag aaaggcgtta tgcctgaatt ctcatttttt cagaatatcg
    13561 caaaagctca cacaatgtgc ggagaaaata aaaacgggca gccattgcca cccgttttca
    13621 gaatccnnnn nnnnnntatg taagattaga tagcccagcc acccgcatag aaggtcacca
    13681 gcgccacggc aataacgacc gtaccgatgt tcaacttgcg ccattcgccg gaaaccagac
    13741 gaccaatcac cagagtcgcg aagccaatca tgatgcctgt tacgatgtta caggtcagca
    13801 cgatgaatac cgccgtaacc agacccgcca tcgcatcaac aaaatcagca aagtcgattt
    13861 tcgccacgtt gctcagcatc agcaggccaa cgtacatcag cgccggagcc gttgcgtacc
    13921 ccggaacgag gtaagagagc ggagagagga acagaatcag caggaacagc acgccaacgg
    13981 tgatagccgt caaaccggtt ttaccgcccg ccgccgtacc cgccgcagac tcgatgtata
    14041 ccgctgccgg agctgcaccc accaggccag aaaaaacgct gctcatggag tcggtggtca
    14101 gtgctttccc gccgtcgatg atctgcccat ctttatccag caggttcgcc tgaccggcga
    14161 cggcacggat agttccggtg gcatcaaata ctgccgtcat caccagtgcc agaacacttg
    14221 gcaggactac aggattcagc gcgcccataa tgtccaggct gcctatcagt gaattgccgt
    14281 tttcatcgct caatgaaggc atggcgaaaa cgccggagaa atggacgtta ggatcgaaga
    14341 tcaaaccgac aattgagata ccgataatgg tcagcagaat gccaccaggg actttcagtt
    14401 tttccaggcc gataatcact gccagcccca ccaacgacat catcaccgga aaggtcgtga
    14461 aatcccccag cgcgacgggc agaccatcaa gtgggttttt aatcaccaga ccgacaccat
    14521 tagcagcaat cagcagcagg aacagaccga taccaatccc cgtgcnnnnn nnnnnccgtg
    14581 cggcaagttg cgcaaaatcc agctacggat acccgtggca gaaattaccg taaacagcac
    14641 acccatcagg aacacggcac ccagcgcgac aggtacgcta atatgttgcc ccagcaccag
    14701 actgaatgcg gtaaacgccg tcagagagat ggcgcaacca atcgccaacg gcagattagc
    14761 ccacagaccc atcacgatag aaccgagtcc ggcaaccaga caggttgcaa cgaaaactgc
    14821 cgcaggcggg aagcccgctt tacccaacat gcctggaacg acgataaccg agtagaccat
    14881 cgccagaaac gttgttaacc cggcaaccac ttcctgacgg acagtgcttg cacgttgtga
    14941 aattttaaac caggcgtcga gtgaaccgcc ggtacgcgct gatggcgtag acataagaaa
    15001 acatcccctg agagtttaat tttcgtcaag atgcgtggcg gacaatcccc tgccagcgaa
    15061 acgttatctt tctgctccag gtttgcgaca atttgctgcg tcgcaaaaaa agcaaacgtt
    15121 tagctccgcg catacaaaac gggtggtaaa aaaagcaaac gattatccgg cgatcatagc
    15181 ggctttttca accgaagttt gctgcttttt tctcttttat tgtcttgacg atgaaaaacc
    15241 aggcaattga tgcgcaaacg ttatcgtcga tgcgcaagat tgcagcgtta tcgagccgtt
    15301 taccgttcag gattgcgata aaaaatcgtc tgagatgaca aaaatattgc ggctaaaaag
    15361 tgtactttta cactggcgaa gaaacgccgg taacatacaa ttaaagcatc aacaccaacc
    15421 ggaacctcca ccacgtgctc gaatgaggtg tgttgacgtc gggggaaacc ctcctgtgta
    15481 ccagcgggat agagagaaag acaaagaccg gaaaacaaac taaagcgccc ttgtggcgct
    15541 ttagttttgt tcatcttcca gtaagcgtgc gccggtacct tcttctccta atcggtcgcc
    15601 cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac atccatccag
    15661 ttcgtcacgt agcgccacta aggtatgaat gcgccgatcc aactcttctc gccattggga
    15721 cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca cgccaaacgc
    15781 ttcacgaatg gttgcgagcg gaatgccaat acgctgagca attttgataa ttgcgacata
    15841 tcgcaacaca tcacgtttat atcgccgctg attgccgcta ttacggatac tggtaatcaa
    15901 ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt tcgccacttc
    15961 gccgggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc gctttacctc
    16021 aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa cactgaaaag
    16081 aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga ttgacgagca
    16141 tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tctggctatt caaagtggta
    16201 cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca ttcgccagcg
    16261 ccgtctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt ttgatatcgc
    16321 aatggacctg ggttatgtct ctcagcagac cttctcccgc gttttccgtc ggcagtttga
    16381 tcgcactccc agcgattatc gccaccgccc gtagctttat tnnnnnnnnn nccccatcca
    16441 ttgcataaac acctgcggcg gcatcgcctt cgcaaaaaac catccctggc aatagcgcac
    16501 gccgtgtttg cgcagccagt taacctgcgc ctcagtttcg acaccttcag cgatcgtttt
    16561 taatcccaga ctgtgcgcca gctcgatgat gtgttccgca atcaaatgac tggttttgtg
    16621 ggtggtcagc gtctcaacaa acgatttatc gattttcaaa atatcgacat tcaatgattt
    16681 aaggttatgc aagttagagt agccaatacc aaaatcatca attgccactt cgtaaccggc
    16741 cttgcggaaa gccagaataa tcggcgtcat tttgtcgaca tcaagaaatg catgttcagt
    16801 nnnnnnnnnn acttcaaatt taatttgctg cgggcgcacc gcatgttgct ctgttttctg
    16861 attggttcgc gctatcaacc gtgaggtatg aaaatcggag gctgacaggt taatagaaac
    16921 atagcgatcg gtatgtgttg ccagatacgc gcccaaatca cggaagacat tatcaataac
    16981 ataatcagtt acctgttcga tcatcccctc cttttctgcc agcggaataa actctgccgg
    17041 attcattact tgcccctgct caccgggcca acgtaacaac gcttcagcgc cgatacattt
    17101 ttctgttttg atatcgatta ttggctggta ataaaggcaa agttgatgtt tttcgagggc
    17161 gcgttgcaat ttgcgtttgg gagacaaata gttttgtcga atacgtagcc agagtaatag
    17221 cagaacaaga ctccccaaaa taccggcggg caacgcaaaa ataagatgat tataaaaatg
    17281 agttataaga cgttgatatg aagtcgcaac aatggctgca attgggcgtt ttgtcgaata
    17341 aactgtcgca tataaatagc catttcgttg tacggttaaa tcattcaaat gaatcagcgg
    17401 agaaaacgtt gctgccgagg cctcgttgct taacgagaaa aaggttttcg tcaccgtatc
    17461 atacacaccc cattgcaatg tcggatcatc agacatcact tcactccaga agagagggtt
    17521 gataaccgcc acataatttc cccgctgcat gtaggtcatt ttatagccag agaaaaaagg
    17581 cgtatcacgg taataataga tagaaacgtt aggttcacgc ttataatcgg ccggtgcaat
    17641 cgtatagcca tttacagacg ctatcagcga tgagcataaa aaatggttat cacgagcata
    17701 aatcaattca ttaatataaa gatagccacg aataatattc aacattcgct tttgatgggc
    17761 tggagtgcaa acttgcccct gatagcgttc agctgcatcg ctcactaaat ctgcctgacg
    17821 aatgaccagc tcagatttat ccaacgccag ctgagcaaag gttcggagct gattattcac
    17881 ttctgatttt gcccaaaaga aggcaatcca tagcgacaga atgatgggaa agagaactaa
    17941 aaagataatg cccgaaaacg ccagtaattg gtgtcgtgca cgatgactca ttgctatcaa
    18001 ttccctgcct gtttataccc ggttattgca aggtgaaacc ctggctaccg acgtctttag
    18061 ataaatctta cggtataaat agcacttttg cctgatataa aattgagacc tttttaatcg
    18121 tcaccaggat gacgataatt cattgatctt aaaaattaat caccttcagg aatgcagagg
    18181 cggcgggaag atataccctt aatgccgctg aaaaaacaca taatctgtac ggcaataaaa
    18241 gtcacggcct aatctggcta acaaacaacc aattgtgata tagttcacaa aattcatgaa
    18301 acaaacagag tgtttcattt ttgtgttctg caagccccgc gatagccaga gtgtatcacg
    18361 cctcccgtga acaacgccgc gctgtccagg gcatcagctc ttttccgagg aattgtttga
    18421 tggctaccct caccactggc gtggttcttc ttcgctggca acttcttagt gccgtaatga
    18481 tgtttctggc tagcacgctc aacatccgtt ttcgtcgggc tgattatgtc gggcttgcag
    18541 tgatcagcag cggtctgggc gtagtttccg cctgctggtt cgcaatgggg ttgcttggca
    18601 tcacaatggc ggatatcacc gccatctggc acaacatcga gtcggtgatg atagaagaga
    18661 tgaatcagac accgccacaa tggccaatga ttttgacttg atatgtagaa gcctccataa
    18721 cggaggcttc ttttttatgg ctggcagatg ctttaatcat ccaccttaaa acaatatacc
    18781 ctattgtttt aaagacaaat cagaacggaa tatcgtcatc aaagtccatc ggcggctcgt
    18841 tagacggcgc tgccggagcg gactgctgcg ggcgagactg cgcgccgccg ctgaactgat
    18901 tgccaccctg cggctgctga ggctgacccc aaccgccctg cggctgacca ccaccgatat
    18961 tgccacctgc aggagcgcca ccaccctgac gaccacccaa catctgcatg gtgccgccaa
    19021 cgttcaccac gacttctgtg gtatagcgat cctgaccgga ttgatcggtc catttgcggg
    19081 tacgcagctg gccttcgata taaacctgag aacctttacg cagatattcg ctggccactt
    19141 ctgccagttt gccgaacagc acaacgcggt gccattcagt ctgctctttc atctcgccgg
    19201 tcgctttatc acgccaggat tcggaagtag ccagcgtaat gttggcaaca gcgccaccat
    19261 ttggcatgta gcgtacttcc gggtcctgac ccagattacc aacgagaata accttgttta
    19321 cgcctctgct ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa gtgtaaacgc
    19381 gcgattgtac cattaccaat agcgctttta ctatgttgtg acctcggttc cgggaaacaa
    19441 acctggccag acattgttac acaacactcc gggtaatgca ttccaatact gtatattcat
    19501 tcaggtcaat ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg
    19561 caaaaaagcg tttaatccgg gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca
    19621 cccataatct caaaaacatc aacctcgtta tcccccgcga caagcttatt gtcgtgaccg
    19681 ggctttcggg ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc gaagggcagc
    19741 gccgttacgt tgaatccctt tccgcctacg cgcggcagtt tctgtcactg atggagaagc
    19801 cggacgtcga ccatattgaa gggctttctc ctgccatctc aattgagcag aaatcgacgt
    19861 ctcataaccc gcgctctacg gtggggacaa tcaccgaaat ccacgactat ttgcgtctgc
    19921 tgtacgctcg cgttggtgag ccgcgctgcc cggaccacga tgtaccgctg gcggcgcaaa
    19981 ccgtcagtca gatggtggat aacgtgttgt cccagccgga aggcaagcgt ctgatgctgc
    20041 tcgcgccaat cattaaagag cgcaaaggcg aacacaccaa aacgctggag aacctggcaa
    20101 gccagggtta catccgtgct cgtattgatg gcgaagtctg cgatctttcc gatccgccga
    20161 aactggaact gcaaaagaaa cataccattg aagtggtggt tgatcgcttc aaagtgcgtg
    20221 acgatcttac ccaacgtctt gccgagtcgt ttgaaaccgc gctggagctt tccggtggta
    20281 ccgcggnnnn nnnnnntgct gttctccgcc aactttgcct gcccgatttg cggttacagt
    20341 atgcgcgagc tggaacctcg cctgttttca tttaacaacc cggcgggagc ctgcccgacc
    20401 tgtgacggtc ttggcgtaca gcaatatttc gatcctgacc gcgtgatcca gaatccggaa
    20461 ttgtcgctgg ctggcggtgc gatccgtggc tgggatcgcc gcaacttcta ttatttccag
    20521 atgctgaaat cgctggcaga tcactataag ttcgacgtcg aagcgccgtg gggcagcctg
    20581 agcgcgaacg tgcataaagt ggtgttgtac ggttctggca aagaaaacat tgaattcaaa
    20641 tacatgaacg atcgtggcga tacctccatt cgtcgtcatc cgttcgaagg cgtgctgcac
    20701 aatatggagc
//
LOCUS       DAWDRC010000006        20396 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1432_length_20396_cov_2.623666, whole genome shotgun sequence.
ACCESSION   DAWDRC010000006 DAWDRC010000000
VERSION     DAWDRC010000006.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20396)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 20396)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1432_length_20396_cov_2.623666"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    339..348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8108..8117
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11955..11964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12395..12404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5067 a         5215 c         4791 g         5283 t
ORIGIN      
        1 tcgtcagaaa cggccatttc gccagtgtag tcggttgatg ccacccacag acgcagaata
       61 tccgcaccca gtttgttcat cacatcctgc ggcgaaacgg tgttaccgat ggatttagac
      121 atcttgcgac cctgaccatc cacggtaaag ccgtgagtca gtacctgacg atatggcgct
      181 ttgcccttca tcgcggtaga gatcatcagg gaagacatga accagccacg gtgttggtca
      241 gaaccttcca gatacatgtc cgctgcgtga ccggcaaatt ccggacgcac gtcaacaacg
      301 gaagagtggg tagatccgga gtcaaaccat acatccagnn nnnnnnnnga tcagcttcat
      361 cgccgaggat ctctttcgca tcgagatccc accacgcctg gatgccgtca acttccacgc
      421 gtttagccac ttcttccatc agttcgaggg tacgcggatg cagctcttcc gtgtctttat
      481 gcacgaacag tgacatcggt acgccccagg tacgctgacg agagatacac cagtcaggac
      541 ggttagcaac catcgactcg atacgcgcct ggccccagtc agggatccac tgcacgcctt
      601 tgatctcttt cagtgactgc gcacgcagac ctttctgatc catgctgacg aaccactgcg
      661 gcgtcgcgcg gaagatgatc ggcgttttat gacgccagca gcacggatag ctgtgttgca
      721 ttttctcaac gtgcaacagt gcgccttttt cctgcaacaa cgcaatgaca atatcgttcg
      781 ctttgaagac gttaacgcca tccagagtcg ggtaagtacc cggcagataa gttccgtccg
      841 ggccaaccgg gttagcggtt tccagaccat atttttgacc gatcacatag tcgtccggac
      901 cgtggcctgg cgcggtatga acggcaccgg taccggcatc cagcgtaacg tggtcgccga
      961 gaattgccgg aacatcgaag tccataaacg gatgggtaaa gcgcaacagt tccagctcgg
     1021 cacctttcac cgtgccaaga atggtgtaat cgctaacgcc gatacgctgc attacgcttt
     1081 caaccagatc tttcgcgagg atcacggcct gaccgtcgat ttgcaccagc gcataatcaa
     1141 aatcaggtgc aatggagatt gcgcggttag caggcagcgt ccacggcgtg gtggtccaga
     1201 taaccagcga aattgggccg ttaacattgc ttacgccaaa tttcgtttta agcgcatcct
     1261 gatcgaccgc ctggaaagcg acgtcgatgg acggagaagt tttgtcgtaa tactcaactt
     1321 ccgcttctgc cagtgcagaa cggcagtcaa cgcaccagtg caccggcttc gcgcctttgt
     1381 gcaggtgacc gttgccgatg attttgccca gcgcgcggat gatgttggct tcagttttga
     1441 agtccatggt caggtacggg tgcgaccagt cgcccagcac gcccagacgg ataaagtctt
     1501 tgcgctgacc gtcaacctgg gtcgcagcgt attcgcggca cttggcgcgg aactcagcgg
     1561 cggtgaattt ctcccccggc ttaccgtatt cttgctctac tttcagttcg attggcagac
     1621 catgacagtc ccagccaggc acatacggcg agtcatatcc agaaagccct ttggacttaa
     1681 tgataatgtc tttcagaatc ttgttaaccg agtgaccaat atgaatgctg ccattcgcat
     1741 aaggagggcc atcatgcaga atgaaggttt ttttgccttt tttagctgca cgaatgatgc
     1801 cgtacagatc atcatcagtc caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc
     1861 cacgcatcgg gaaccctgtt tccggcaaat tcagggttga tttatagtca ctcatcagat
     1921 tctcggttcc gtatttcggt ttgattacat aacaggctta agccggtttt gttagcccaa
     1981 aaaattcgcg ggcggttaat tcatcacgcg caatctgcgc tttcagttcg tccagcgatg
     2041 caaatcgctg ctcattgcgt atttttttac gcagcactac ttgtatatgg cgaccataaa
     2101 ggtccattgc aacatctaac aaatgcactt ccagttgctg gcgaataccg gcaaccgttg
     2161 ggcgtgttcc gatgtttgca acgccgggta acggcttttc gccaaggccc aacacttcta
     2221 ccgcataaac ccctttcacc ggggaaacct gacggcgtag cggtacattc gccgtcggga
     2281 aacctatagt gcgccctaat tcatcaccgt ggactacacg cccggagata gcaaacgggt
     2341 gccccagtaa actttctgcc agagccagat tgtcatccgc aagcgcctga cgcacggcgg
     2401 tgctgctgat acgcacgcca ccttcgcaaa aagtttgcgt gctggtgata tcgaagccgt
     2461 attccatgcc cgctttctgt aataacaaga aatcgccttc acgaccagcg ccaaagcgga
     2521 aatcgtcacc tacggcaaga aattttaccc gcaagtgctt caccagaaga tcactgatga
     2581 agttttgcgc ggttaacgcc gcaaaacgcc tgtcaaaacg cacgcacagc acgtaatcaa
     2641 cgccacactc tgcaagataa cgcagttttt cccgcagccg ggtgagccgt gccggggctt
     2701 tatcagtagc aaacagttcc agtggttgag gttcaaaaag catcaccatc accggtaagt
     2761 tgcgcttgcg cccttcttcc tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc
     2821 cgtcgaaatt accaatagtc agcacacacc cttcttgcgg ggcctggctg agattatgta
     2881 tgccgcgtat cagcttcatg tctggctcaa aacagtgaaa atcgtccgag tataccttgt
     2941 acagcggtca aggttaaccg gcgattgagt accgaaatca gaaagaagtg gcttttcatt
     3001 gctatggcgc aaatcatggg aagaaactga ccgcctgctg caatttttct cgcggaaaag
     3061 ctgtattcac accccgcaag ctggtagaat cctgcgccat cactacgtaa cgagtgccgg
     3121 cacattaacg gcgcttattt gcacaaatcc attgacaaaa gaaggctaaa agggcatatt
     3181 cctcggcctt tgaattgtcc atatagaaca catttgggag ttggaccttg gctaatatca
     3241 aatcagctaa gaagcgcgcc attcagtctg aaaaggctcg taagcacaac gcaagccgtc
     3301 gctctatgat gcgtactttc atcaagacag tatacgcagc tatcgaagct ggcgacaaag
     3361 ctgctgcaca gaaagcattt aacgaaatgc aaccaatcgt ggaccgtcag gctgctaaag
     3421 gtctgatcca caaaaacaaa gctgcacgtc ataaggctaa cctgactgca cagatcaaca
     3481 aactggctta attgcctgtt gttgtcagct ttgtaaaaaa acccgctctg gcgggttttt
     3541 ttatatccat ctgcaggcca aaattcggcc tgcaaagtac aaatgtttat tttgaagctg
     3601 gagtaaacag cgcagaatag tctgtattgc agatacgctg tactgcaggg tgctgaatca
     3661 tcctttcggc aaaaatcgcg tggtactctt ccatcacgtt ctcaacacgg cctatctcca
     3721 caaccgagtc atcgttatgg aaaacagccc ttcctgctga gtggaatcga tcggacagtc
     3781 agagatgatc atatccagtt tatgctgact caactgctct aaaagcatct cgtgcgtcga
     3841 ttcgaaacag cgtagatgga tctgctcgtc ttccacaact gcggcatcca gaacactgct
     3901 gaccagacgt ttggaaagtg catctgccac accaacatca aacaataagt tggactcttt
     3961 gcgatagttg acgatatcca gcatttcctg gcttaaggtg aacattttat cggcatagcg
     4021 atagaccagt tcccccagtt cgctgggttc cagaccacgt cctttacgct taaatagttt
     4081 cccttgcagg cgctcttcca gcgcccggat ctgcccggta atggtttgtg gtgttaaata
     4141 aagcgcctcc gctgcgccaa ccacagaacc ttctttgtag acatgccaga agtaatacaa
     4201 gtggttgtaa ttgatatgag acatgctcat ttctctccct gataacaatg aaaagggagc
     4261 cgtttatggc tccccagtac atcgtcctgt caaactgatg gacgtaaacg aacgcgtaac
     4321 cagctatatc caattaccgc cgaagaaatt gaaccgacaa ggatacctaa ttttgcccag
     4381 ttaatcagtt ctggatctac gctaccaaat gccaggctgg caataaagat agacatagta
     4441 aaaccgatac cgcacaggat accaaccgcc ataatttgct ggtaagtcgt tccctctggc
     4501 agatgtgcca atttcaaacg cagcgccaac cagcagaaca gactaatacc cagtggcttg
     4561 ccaatcagca aaccagcgat gatccctaat ggcaggatgg aggtcaggcc atccagcgtg
     4621 acgccttgca gtgaaacgcc agcattagca aatgcaaaca gcggtaaaat cagatacgcc
     4681 acccacgggt gcaacacatg ctccagtcgc ttcgctggag aacgcccatg cttctctttt
     4741 aacggaataa agaagccgac aatgacgccc gccagggttg cgtgaacccc cgatttcaac
     4801 accgctgtcc acagcaccac gccaaccaga atataaacgc ccgtgcggcg tacaccacac
     4861 agattcaata ccacgagtac cgcaattgct acagccgcga cgccaagaga ggccatcgat
     4921 aagtcattag tgtagaacaa tgcgatgata atgatggccc caagatcgtc gataatagcc
     4981 agagccatca aaaagatctt cagcgctaac ggaacacgac ttcccaacag cgccaacaca
     5041 ccaagtgcaa aggcaatgtc agtcgccgcc gggattgccc agccttcgcg ggtaatcgga
     5101 tcggcatagt taaaagccag atagagcaat gccgggacaa tcatcccgcc gattgcggca
     5161 ataacaggaa atgccgcctg gcgcagactg gccagcgaac cttgcatcag ctcgcgttta
     5221 acttccagac caaccaacag gaaaaatacc gccatcagag cgtcattgat ccatagcagc
     5281 atgttcttgt tgatctcaag tgtcccaacc cggagctgaa ccggcgtctc aagaaagtcg
     5341 tgataccatc cactggttgc accgctgttg gccataatca tcgctaatac agcggcaata
     5401 atgagaataa tgcctcccga ggcatcactg ctaaagaatc gatgcagatg tttcactttt
     5461 tatttctctt caggtgaata gatcgttttc acgattttac cccgcacgaa tcatcgttaa
     5521 aatcagatta tagtgtagga atagctcgct ttttacgatc tattaaaaga attgcgccat
     5581 cagcgagaga tgagtttttg atgcccgaaa gcggcggctg atgaccttaa accccattaa
     5641 tcatagtcta atgtcaagat ttctgaagaa attattcgga aaagtaataa ttaaattgtt
     5701 ccatgctcta gttttttatt ccacagatgt ttgaggatat ctaatgtaga aacgacattc
     5761 gcaggagaca ttcggttcaa tgatataagg aaaggatggt tctccacaaa gtggataaaa
     5821 cgcgtcgcac tgttcaatgt tgcatgccgc atctcgcgga ctttcttact gccagtgaga
     5881 ttgcatacag ccattgaata attgcggtta gaaaatgcac gccagacatt atttcgtacc
     5941 ccaccactaa ggaataatcg cggtggaaaa tcaatggtgg cgggtgccac accgcgctct
     6001 ttgacaaagg aaatatggca gcgaccttga tgaatatgct gcaaattagc catcaggcag
     6061 ccgcccaata taaagcattc gtgatcaagg taatgctgaa cgttgttgga atgctcatcg
     6121 gtcagtattc tgaccagttc aaaatgacga tatgccggaa gaaccggttt taccaacatt
     6181 cccggatcgt tttgatgtaa aactgccggg ccgtaatcaa tatcaaacaa ggtatcccgc
     6241 cgcatagtaa gtaactcttt gcgctggaca tgatgtgcca gcgtccctgg agacatacgc
     6301 tcttcatagt tcgatgtgta ttgataatgt tgctctacgg cagatgggga gtaattggtt
     6361 gagatggcaa ccaccctgcc gctttgttct tctgcggtaa caagagcata aagagagtta
     6421 ttgctaccat tatacttgac gcgaaaagcg cgggtactca gggtaatatc agaagctggg
     6481 aaacactgtc gactttcttt atagtttgct tcacgcgcta attttaacag ttcacggcta
     6541 agtccagtgc tgttaagaag taatgacttt ctgatatccg caagtgcctc cccttgctca
     6601 atcatcaatg ctaacagggc tcctcgtggt gtggtaatta catgttccag agtgataaat
     6661 gttttctcac aatgatggca atacattctt ctctggcctt gtgcagaata tccatatttt
     6721 ttgagggacg tgcctccgca agttgaacat tggcaaatca aacctctcca ggagtgattc
     6781 acaatattac gataaatatt aagcgactgt tcagatatca ctggaaacaa gtaaccacat
     6841 tcacggcata aaatattttt accctgtgcg acataatcct ggctattcag caaccccagg
     6901 tttttacatc ctggtgtttt gcaacaatca acatttacgt tggtaaacat atccactccc
     6961 tgcgaatagc cgtatcttga tattgttcaa cgcgaaaaga acctgattca atgcgtagca
     7021 ctgaatcagg cgggcatagc cctaaatggc atacattaca tttattatga ttatcaggtt
     7081 agccccaggt caccgcttct ggtttatcac ctctggcttt cattagagaa tcattatcaa
     7141 tatcagtttt atattttaaa aacttaccat acatctgttt ttcaatatct gtttttttac
     7201 cgatcagatt aagtgtttca taacgatcag atttcagatt gtagagatat ttcggtttat
     7261 tattgcgatc gataatcatc ttccagtcac catcacggat cgcccattca tcggttggat
     7321 catcctggaa tggcatatca atcccgaaaa ttaatggctt ttcgcgtttc aatgcttttt
     7381 gctcaagaac aggaaccagc gattcaccat cgaaagtacg gtctgtaggt aatttgaagt
     7441 tcatcatttt cgctaaagta ggcatccagt ccagaccata aacgggtgta tctgaaacca
     7501 ttccctgtgg tagatgttta ccatatttaa taatggctgg aacacgaatt ccgccttccc
     7561 aaaggttatc cttgcgaccg cgtaatccat ccgtttcccc tgccaaattc agctcataca
     7621 ctttgcgcgc ttcacgcgtt accggaccgt tatcactggt aaaaataacg attgtgttat
     7681 cttcttcacc catcgctttg attttatcca gcacttttcc aacctgtgca tccagatagc
     7741 tgatattggc ataatattcc cccacaccac gccagggttt gtctgcccag tcgccataaa
     7801 ataaatcagg atgctgcttc tgatacgcgc tcatatattg tgagtacatg tcgaggtatt
     7861 ttttgggcga agccaggggg ctatgcactt cggtaaaagc aacatagagg aagaaaggct
     7921 tgctgtcctt tttgttatcc agccagttga cgacttccga actgacatac tcaccgctca
     7981 ttttatcagc tcgtggagtg ggttgcccgt tacgtagcca gcctgtcggg taaaccatgc
     8041 cataacgcgg gcgttcttta gcgttatcca gcgtggcgtc ggtaacaaag cccgccgtat
     8101 taaccagnnn nnnnnnnaac cagtgagtaa tcaaagccca tatcttgtgc ttgtggctga
     8161 tcggtacgat cgccgcctgc attcagatgc agcttaccca tcattgccgt gtcgtaccct
     8221 tgcgctttga gtagattagc aatcgtgagt tcgttacgcc ctaaggcaac atctttgcct
     8281 gaaggaatcc atgagcgaat tccagtacga aatggcatcc ggccggttaa tagccctgcg
     8341 cgtgaaggtg aacttaaagg agcgggggca tagtagtcag taaatttgac cccttcctgg
     8401 gcaagcctgt cgatattagg tgttttaacg atctgatgac catatgttgc taaatcgcca
     8461 taacctaaat catccgccat aataatgact aaattaggtt gtttagcctc tgcggctaag
     8521 gcaggactaa aagcattccc tgtgcaaact gcaaggccga tcaaactggc cattaacgtt
     8581 ttctgcataa ttattccctg ataaaaatta gaaatcataa ccaagcatat aaatcatttg
     8641 atcgccaaag ccatcgtagc ccagcttatt atcgaaataa cgccacgtca cactggcttt
     8701 ccagcgagga tagaacttcc aggcaatagt taaaccgcca tttaacccgt tccggccaaa
     8761 ttgctgctcc gtgtaagcat cgttcctgtc cagttctatt tcattccagt tagataaaac
     8821 aaatttttcg tcaaataacg taaatggtaa tactgctgtc cagccaacaa cataaccatt
     8881 ccaaccattc gtttgaccat atttagctga tacgtagtca ccagattgat tatgcaatcc
     8941 aatatacggt ttaaaaaagc cccactcgcc gttccagccg aggtaaccga atccataaaa
     9001 catgtccaga tcgtcacccc atgagttatc ccattggcca taaattttgc caaagaatgt
     9061 catatcagag tcaaagagtc tgacatgcgt cattgctgaa accgtatgat ttctccctgc
     9121 tacagcatga ttgagtacat tactttcata aaatgagtaa aattctgctt ctttaaaatt
     9181 taccccaaaa tccgcagtca ctttccatac ttcaccacgg tgagtgttta caaatccact
     9241 attccagtct gcataacccg catttattga accaaaggtt cctttatatt ctaatgaaga
     9301 atatgcagga aaatgataga tagctgttat caaggtacac gcaataagaa gtcgtctttt
     9361 ggcagacata taatatgttc tccattcatt gtttttatga ttccatagga attaatacac
     9421 cagacaattc atttttatta agaatgacca ggccagtata tttaaagatg actatcgaag
     9481 cgtgatgaag atcgcagatt acttttgcga acgggcggag ggatatcgtg tttcagaaaa
     9541 tgtgtctgtt ttaaacatca ccctttgatt tataggcatt tttcatgatg aacttagcat
     9601 tggctgctga acgtatttct ttgtctctct atagtattac agtaaccttt aataattaat
     9661 ttcctttagc cattcacttt tgatagagga aaacaccttt ttttgtgaat tcctggatat
     9721 atttaagtta aataaattga tgtattgaaa tattattccg caaaaatgcg aggcgtcttc
     9781 agaaaaagaa ttgataaaac agaagtgaat taaaagcgca gtataatagc ggcgggtgct
     9841 tgaggctgtc tgtctcaggc attagctgaa cggcagatag agaaaagccc cgagtgatat
     9901 tttaccatca acccgaggcc ccctatatgc agaacacatg tagagtgcct cttactgacc
     9961 gaaaggtcaa ggagaagaga gcaatgaagc agcataaggc gatgattgtc gccctgatcg
    10021 tcatctgtat caccgccgta gtggcggcgc tggtaacgag aaaagacctc tgtgaggttc
    10081 acatccgaac tggccagacg gaggttgctg ttttcacggc ttacgaatcc gagtaagagc
    10141 aacggcgggg agtgatcccc gccactttac aggtgctcgc atatcttcaa cgcaccctgg
    10201 tttttaccct ggcctgccca cgggcaggct tttggggagg ttagcgagtc aggtcgtcaa
    10261 aaaacttctt cacgccatca aagaagctct ttgaacgcgg gctgttgtgc tcgccggttg
    10321 ggccaccaaa gctttcttgc agctcttgca gcagctgttt ctgcttctcg ttcaaaccta
    10381 ccggtgtttc gacaacaacg cggcatagca aatcaccctg tgcgccaccg cggacagact
    10441 tgacgccttt accgcgcata cggaacagct taccggtctg ggtttcgcca ggcactttca
    10501 gtttgacgcg accatcaagg gtcggtactt cgatttcacc acccagcgcc gccatagcga
    10561 agttgatcgg gacttcgcaa tacaggttgt tgccttcacg ctcgaaaatc gggtgctgtt
    10621 taacctgaac ctgaacgtac agatcgcctg ccggtgcgcc gtgttcaccc gcttcacctt
    10681 cgcccgcaag acggatgcgg tctccagtgt ccacccctgc cgggatttta acggacagcg
    10741 ttttgctgcg ctcaacacga ccatgaccat gacatttgtt gcacggatct ttgatcagcg
    10801 taccgcggcc ctgacagtgt ggacaggtct gctgcacggc aaagaaaccc tggcgcatct
    10861 gcacctggcc agaaccatga caggtcggac aggtctgcgg ctgtgtacct ggttttgcac
    10921 cgctaccgtg gcaaacgtca cactcttcca gagtcggaat gcggatctct ttggtcacgc
    10981 cacgtacagc ttcttcgagg gtgagctcca tgttatagcg taaatcagca ccgcgcgccg
    11041 cacgttgacg accacgtccg ccgccaaaaa tatcgccgaa aacgtcacca aaaatatcgc
    11101 tgaagtctgc gccgccgcca aaaccgccgc cgcccatgcc gccttgctca aacgcagcat
    11161 gaccatactg atcgtatgcc gcacgttttt gcgagtcggt cagaacttca taagcttcct
    11221 tgatctcttt aaatttcgcc tcggcctctt tgtcaccctg gttacggtcc gggtggtatt
    11281 tcatggccag gcgtttgtag gcctttttga tttcacgctc ttccgctgtt ttggaaacgc
    11341 ctaaaatctc gtaataatct tgcttagcca tcttttttga tttgccccta gatgaatgca
    11401 cgggcggaga ggaaattccc cttcgcccgt gtcagtataa ttacccgttt atagggcgat
    11461 tattttttgt ctttgacttc ttcaaattca gcgtcgacaa catcgtcatc tttcgcgttg
    11521 tttgcagaag catcagcacc ggcagtctgc tgctgggcat gttgctgctg ggcgatttcc
    11581 atcagtttct gggaaacctg tgccagttcc tgcattttcg cttcgatagc ggctttgtct
    11641 tcacctttca gagcagtttc cagtgcagtc agcgcagact cgatagcagt tttgtcgtca
    11701 gccggcagtt tgtcgcctgc ttcttcaacc tgcttacggg tgctgtgcag cagatggtcg
    11761 ccctggttgc gagtctgtac cagctcttca aacttacggt cagcttcggc gttagcttct
    11821 gcgtcgcgta ccattttctg gatttcatct tcgttcagac cagaagaagc cttgatagtg
    11881 atcttctgct ctttaccgct gtttttgtct ttcgcggaaa cgtgcaggat accgtcagca
    11941 tcgatatcga aggtnnnnnn nnnnccaggt tgaactgacc caaagatttg ttatcagccg
    12001 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
    12061 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtggtca
    12121 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
    12181 cttttacgtc accagtcaga acaccaccct gaacagcagc accgatggct acagcttcgt
    12241 ccgggttaac atctttacgc ggctctttac caaagaattc agcaactttc ttctgaacca
    12301 ttggcatacg agtctgacca ccaacgagaa taacgtcgtc gatatcagat acggacaggc
    12361 cagcgtcctg cagtgcaact ttcagcggct caatnnnnnn nnnnaacggt ttaccagatc
    12421 ttcaaccagg ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt
    12481 cgcgtctgca gtgatgtacg gcaggttaac gtcggtctgc tgagcggaag acagttcgat
    12541 tttcgctttt tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc
    12601 aatgccctga tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc
    12661 ttcaccaccc aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc
    12721 aacctcgtcg atttcgataa tggaaatatc gaaagtacca ccacccaggt cataaaccgc
    12781 gatagtacgg ttgccagtac ctttgtccag accgtaagcc agcgcagctg cggtcggttc
    12841 gttgatgata cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg
    12901 ctgagcatcg ttaaagtatg ccggtacggt aataacagct tcagttaccg gttcacccag
    12961 gtaatcttca gcggttttct tcattttttt cagcacttca gcagagatct gcggcggtgc
    13021 cattttctgg cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa
    13081 cggcatgatg gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg
    13141 tttaatcgca aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc
    13201 aaccagagtt tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc
    13261 ggcgttctcc agtacacgag gagtggtgcc atccataatc gctacacaag agttggtagt
    13321 acccaggtcg ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc
    13381 atgtggttgt gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt
    13441 ttttgcagac tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag
    13501 ggggagatgc aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag
    13561 acgtgaaact gacggcaatt ttattcatat aaagaatgaa tcgcagccaa ggcattcatc
    13621 aaaaaattgt aataaaaaga aaatattacg tgcctgaaac ttctctttat cagcagtaaa
    13681 ctagtttcat ccccctaccc ctccccacca agagaatcct tatgaaatcc gttattacga
    13741 tttccgccag cctggcgatt agcctgatgc tgtgctgcac ggcgcaggca aacgaccata
    13801 aaatcctcgg cgtcattgca atgtcgcgaa acgaaaccaa cgatctggcg ctgaaacttc
    13861 ctgtttgtcg cattgttaaa cgcatacaac tctcagccga ccatggcgat ttgcagttaa
    13921 gcggtgcatc gatttatttc aaagccaccc gaagcgccag tcagaccctg aatattcctt
    13981 cagaaataaa agaagagcaa accactgact ggatcaacat taacagcgat aacgacaaca
    14041 aacgctgcgt ctcaaaaatc accttttccg gtcatacagt gaactcatcg gatatggcca
    14101 cgctgaaaat tatcggcgac gactaacgcc agaatataga aaccacaaaa aatgaatgtt
    14161 aattacctga atgattcaga tctggatttt ctccagcatt gtagtgagga acagttggca
    14221 aatttcgccc ggctgctcac ccataatgaa aaaggcaaaa ctcgcctctc cagcgtactg
    14281 atgcgcaacg aactgtttaa atcgatggaa ggacatcccg agcgacaccg ccgcaactgg
    14341 cagctgattg ccggagaatt acagcatttt ggtggcgata gtatcgccaa caaactgcgc
    14401 ggacacggta aattgtatcg cgccattttg ctcgatgttt caaagcgatt gaagctgaaa
    14461 gccgacaaag agatgtctac gtttgaaatt gagcaacagt tactggaaca atttctgcgt
    14521 aatacctgga agaaaatgga cgaggaacat aagcaggagt ttctgcacgc ggtcgatgcc
    14581 agggtgaatg agctggaaga gctgctgccg ctgctgatga aagacaaatt attggcaaaa
    14641 ggtgtgtcgc atttgctttc cagccaattg acccgcattt tacgcaccca cgcagcaatg
    14701 agcgtgcttg ggcatggttt gctgcgcggc gcgggtctgg gaggccctgt gggcgcagca
    14761 ttaaatgggg ttaaagcggt cagcggcagt gcctatcgcg tgacaattcc agccgtactg
    14821 caaatcgcct gcctgcgccg gatggttagc gccactcagg tctgataccg gaaaccgggg
    14881 aaaattttca ttttcccctc aaaagatcgt agacactgcc ccactggctg attattatgc
    14941 cgcgccctga aaacactaca gttattcagg gaaattattt caccattcat tcgatgatga
    15001 tttttgagga attatgggca acactaagtt ggctaatccg gcaccgctgg gcctgatggg
    15061 cttcggcatg accaccattc tgcttaacct gcacaacgtg ggttattttg ctctggacgg
    15121 tattattctt gccatgggca ttttctacgg cggcatcgcg caaatttttg ccggtctgct
    15181 ggagtacaaa aaaggcaaca ctttcggttt aaccgcattc acctcttacg gttctttctg
    15241 gctgacgctg gttgcaattc tgctgatgcc gaaactgggg ctgaccgatg cgccaaatgc
    15301 acagttcctt ggtgtctacc tgggtctgtg gggcatattt acgctgttta tgttcttcgg
    15361 cacgctgaaa ggcgcacgcg ttctgcaatt cgttttcttt agcctgaccg tgctgtttgc
    15421 cctgctggcg atcggtaaca ttgccggtaa cgccgcgatc atccactttg ccggctggat
    15481 tggtcttatc tgcggtgcca gtgcaatcta tctggcgatg ggtgaagtgc tgaacgagca
    15541 gtttggtcgc accgttctgc cgattggtga gtctcactaa ttgttaagct ccccccgctc
    15601 gaagggataa gattgttaac aacgtgcgct ttgtttatgc cggatgcggc gtgaacgcct
    15661 tatccggcct acaaaatcgt gcaaattcaa tatattgcaa gaagatcgta ggcctgataa
    15721 gcgtagcgca tcaggcagtt ttgcgtttgt catcagtctg atccccccgc ctggggggat
    15781 ttttttattc gctaacttcg cgtcttgcac tcttcggtct gaatgctgca accacttccg
    15841 gtgccgtttc aacgtatggc ccttccagca actgaatgca gtacggtacg ctggcaaaaa
    15901 taccgtgcac cacaacgtta ccctcagcgt ccttcacacc ttccagcgtc tctttaatag
    15961 acttcggttg accgggtaag ttaaggatca gcgcctgttt gcgaatcacc cccacctgac
    16021 gcgaaaggat cgcagttggt acaaaatgca ggctgatctg gcgcatctgt tcaccaaagc
    16081 ctggcatctc gcggtccgct actgccagcg tcgcatcggg cgttacgtca cgacgcgccg
    16141 ggccagttcc gcccgtggtg agcaccagat ggcaactcat ttcatccacc agctcacaca
    16201 acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg ggtttccagt tcaaacggcg
    16261 tggttagcgc cgatgtcagc cattcttcca gcgcagggat gcctttatcc tgataaacgc
    16321 cgctggatgc gcgatcagag atggaaacta agccaatacg taaagtattc atcaagattt
    16381 ttccgtttaa acagtctcgt taaacagaat gatacactgc gaagggagtg acagacaggt
    16441 ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa gtatgattac agcagatcgc
    16501 cgatcatttt ttccagtttt tcctggtcaa cagcaaactt acggatacct tccgccagtt
    16561 tatctactgc cattggatcc tggttgtgct gccacaggaa ctcggactca gtgatacgcg
    16621 ccggacgcgc tttcacttca ccagtgtaag acagtttacg ttcgatagcc ccttcgcttt
    16681 ccgccagctc tttcagcagt gccggtgcga tggtcagacg gtcgcagcct gccagttcca
    16741 gaatttcgcc gatgttacgg aagcttgcgc ccataaccac ggtttcataa ccatgctctt
    16801 tgtagtactg gtagatttca gatacagaaa ccacgcccgg atcttctgcc ggagcgtact
    16861 ctttcttatc ggtattcgct ttgtaccagt caagaatacg gccaacaaac ggcgagatca
    16921 ggaacacgcc cgcttccgca caagcacgag cctgagcgaa ggagaacagc agggtcaggt
    16981 tacagttgat gccttctttt tccagctgtt ccgcagcacg gataccctgc caggtagaag
    17041 ccagtttgat cagaatacga tcgttgctaa tacctgcatc gttgtagagt ttgatcaggc
    17101 gttttgcttt cgcaattgac gcttcggtgt cataggaaag acgcgcatca acttcagttg
    17161 agatacggcc cggaaccagt ttcaggattt ccagaccaat atttacggcc agtttgtcgg
    17221 tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg tttcgcccag acgacagcat
    17281 catcaatcaa cttacggtat tccggaatct gcgctgcgtt aagaatgaga gaagggttgg
    17341 ttgtggcatc ctgcggttga tacagcttca ttgccgcgat gtccccagtg tcggccacta
    17401 cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat gatagtattt ctctttaaac
    17461 agcttgttag ggggatgtaa ccggtctgcc ctgatgatat cacgacgcaa cgtcagcgca
    17521 accgccgaca agacggttat cgccaggcga gactgtttcg gatttctgac actgcgttga
    17581 tatcgcccgc catttttata caaaacctca tgtatgctac gcagaagtta tcaagtacct
    17641 cgtagcgtat atacttctta aacaattggt aacgtttaca caggaaagtc atcgcgaccg
    17701 gcaataagag ggatatgcat gccagatttt ttctccttta ttaacagcgt cctttgggga
    17761 tcggtaatga tttacctgct cttcggcgca ggttgttggt tcacttttcg caccggattt
    17821 gtgcagtttc gctacatccg ccagtttggc aaaagtctta aaaatagcat tcatccacag
    17881 ccaggcggtt taacctcatt tcagtcattg tgtaccagtc ttgcagcgcg cgtgggtagc
    17941 ggcaacctgg ccggcgttgc gctggcaatt accgccggtg gacctggagc cgtcttctgg
    18001 atgtgggttg ccgcatttat cggcatggcg acctcgtttg ccgaatgttc cctcgcacag
    18061 ctttataaag aacgtgatgc caatgggcag tttcgtggcg gaccggcatg gtatatggcg
    18121 cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg tctttttgct catcgcctac
    18181 ggcataattt tcagcggagt tcaggcgaac gccgttgcgc gagctctgag tttttctttt
    18241 gattttcccc cgctggtaac aggtattatt ctcgctgtct ttgctctgct ggcgatcact
    18301 cgcggtcttc atggcgtcgc ccggctcatg caggggtttg tcccgttgat ggcgataatc
    18361 tgggtactga cctgcctggt gatttgcgta atgaatatcg ggcaacttcc ccacgtcatt
    18421 tggtctattt ttgagagtgc ttttggctgg caggaagctg caggcggcgc ggcgggatat
    18481 accttaagcc aggcgattac taacggtttt cagcgcagta tgttttccaa tgaggcggga
    18541 atggggtcga cgccaaacgc ggcagcggca gctgcgtcct ggactccgca tccggcagcg
    18601 caaggaattg tccagatgaa tcgcattttt atcgacaccc tggtcatctg cacggcaagc
    18661 gccatgctga tattactggc gggtaacggc acaacctaca tgccgctgga aggtattcag
    18721 cttatccaga aggcgatgcg ggtgctaatg ggttcctggg gtgcggagtt tgttaccctc
    18781 gtggttattc tgtttgcctt cagctccatc gttgccaact acatttatgc cgaaaacaat
    18841 ctcttctttt tacgcctgaa caaccctaaa gcgatctggt gtctgcggat ctgcaccttc
    18901 gcaacggtca tcggcggcac cttgctaagt cttccgctga tgtggcaact ggcagatatc
    18961 attatggcct gcatggctat taccaatttg accgccatat tactgctctc gcctgtggtt
    19021 cataccattg ccagtgatta tctacgccag cgtaaactcg gcgtgcgtcc ggtgtttgat
    19081 ccgttgcgtt atccggagat cggtcgccag ctttctccag acgcgtggga tgatgtttcg
    19141 caggagtaat cacaactatc gatcaactca ttctcttttt ttgctaaagt cggcataaat
    19201 ttcctgcaag gactggatat gctgattctt atttcacctg cgaaaacgct tgattaccaa
    19261 agcccgttga ccaccacgcg ctatacgctg ccggagctgt tagacaacgc ccagcagctg
    19321 atccatgaag cgcggaaact gacgcccccg cagattagct ccctgatgcg catcagcgac
    19381 aaactggcgg gtatcaacgc cgctcgcttt catgactggc agccaaattt cacaccggag
    19441 aatgcccgcc aggcaattct ggcgtttaaa ggtgatgtct acactggctt gcaggccgaa
    19501 accttcagcg aagacgattt cgattttgcc caacagcatt tgcgaatgct ttccggcttg
    19561 tatggcgtac tgcgcccgct cgatttaatg cagccttatc gtctggaaat ggggatacgt
    19621 cttgagaatg cccgagggaa agatctgtat caattctggg gagatatcat caccaacaag
    19681 ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga taaatctggc gtcagatgag
    19741 tattttaaat cggtgaagcc gaagaaattg aatgccgaga ttatcaagcc agtgttcctc
    19801 gatgagaaga acggcaaatt taagatcatc agcttctacg ctaagaaagc acgcggtctg
    19861 atgagtcgtt tcattattga aaatcggctg accaaaccag agcaactgac tggttttaat
    19921 agcgaaggtt acttctttga tgaagcttcc tccagcaatg gcgaactggt gtttaaacgc
    19981 tacgagcagc gttaagtttg tagtcaatgc cggatggtaa tccggcattg atatttagcg
    20041 atgatgtttg tccggctccg gacgatgatc gccacgatga tcattgtgct tgttatggcg
    20101 cggcgatggc ggcggtccat atgggtgcca gcgattgcct cgccattcat aatgttgttt
    20161 ccaccagccg tggtcgcgcc agtgaccgcc atcccagtaa tagccacgat tatcacgatc
    20221 gcctatctgt aattttactg acgggactag cgtaatttcc gcagcctgcg ctgccatggg
    20281 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    20341 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acggca
//
LOCUS       DAWDRC010000007        19916 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1472_length_19916_cov_2.714516, whole genome shotgun sequence.
ACCESSION   DAWDRC010000007 DAWDRC010000000
VERSION     DAWDRC010000007.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19916)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19916)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_19916_cov_2.714516"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    694..703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1323..1332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6203..6212
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8159..8168
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9671..9680
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11513..11522
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15792..15801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4508 a         5243 c         5111 g         4984 t
ORIGIN      
        1 cgtacgggcg cggccttcaa tcacgggctc gccccagccg accacgcctt catcggtttc
       61 aattttcagg aacatccagc ggggaggtaa acgatacgtg gtaattttgg tgattttcat
      121 tgcactgcct ctcgatacgc cttaacaaat gctgctgcct gctgcgcggt gcgttctacg
      181 gactgcccgg cgcgatagag atcgctgcct aagcctgccc ctgcacaacc tgcgtctatc
      241 cactgcgcta ggttttctgg cgtcacgccg ccaacggcaa agactgcgat gtccggtggt
      301 aataccgctt ttaacgcttt gatgtattgc ggaccaaaag ccgatgaagg gaatattttc
      361 agtgcctgcg cacccgcgtc gagcgcggta aaggcttcgg tcgccgtcgc gcagccgggg
      421 caaacggtca tgccgtagcc caccgcacgg cggatcactt cactatggat attgggcgta
      481 acgatgagct ggcagcccat cctggcgagc acatcgacct gttcaggttt cagtaccgta
      541 cctgcgccaa tcaacgcctt gtcgccatac gcatcaacga tggcgggaat gctctgctcc
      601 cattgtgggg aattcagcgg gatttcaacc gcgtcgaacc cggcgtcaat caccgcgcca
      661 acatgcgcca gcgcctcgtc gggcgtaatg ccgnnnnnnn nnnaaatggc gatcagcggg
      721 agtttagttt gccactgcat gagcgatgct ccttatacca gcctgaaatg ccgtgtcgcc
      781 cgctaccgcc gtcacgtcac aacccatcgc ctgaaaggct tgctggtagc gcgcggtcag
      841 cgatgttccg gcgacaaggg tgatggcgtg ttgatgggtc acatagtcgc gcatactggc
      901 aacctctgcg ccaatcaaca aaccagagag aaattcgctg acctgttcgc ggggaagcgt
      961 tcccagcaca tgcgaggcgc gaacttcaaa aagctgcggc aatatggcgg gcgcattaag
     1021 gccacgctca aggccagccg cgaaggcatc ggcagagttt tcctgcggcg gcaaacctgc
     1081 gccaaccaat gagtgattta acagtaaatg atgtaattca ccggtcatta cggtgcgaaa
     1141 atcgttgatt tgctggctat cggcctgcac ccatttgcaa tgggttccgg gcataacata
     1201 aagggaggaa ggagccaaag tgcgcgcgcc gatcaattgt gtttcttcgc cgcgcatcac
     1261 attgtggtta tcgtcatgag agacgcataa tccgggaata atccagatat tgccgccaac
     1321 cgnnnnnnnn nntaccactg gcgtattctc ttcacgccag tcggtcgtca cttctgctaa
     1381 caccgcagcc ggagattttc cgttcaggcg cgtgacgcct gcttctgatt gcctgctgtc
     1441 caggcagtgg tcgccctgat aaagccaggc gcgcagattg gtcgatcccc agtcaattgc
     1501 gatgtagcga gctgtcatgt gatttccttt aaccttcgtg tcgagctggc gatcatggta
     1561 agcgccgcct gctctgccgc atcgccgtcc tgatgcctta tcgcatcgaa cagcgcctta
     1621 tgttcctgga gcgtttgcgg catgttggcc tcatcgccca tccaggttcg ttcaaaaacc
     1681 gcccgctgca gcgaactgat cgcaatgctc agttgctgta acaccgggtt atgcaccgac
     1741 tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa acgcttcgcg gtcctgattg
     1801 ttagcaatca tctcgttcag cgccgattca atctgcgcca gatcgctgga ggtcgcgcgt
     1861 tctgctgccc aacgggcaat cgccggttcc accagatttc gcacttcgct cattgcactg
     1921 ataagccgtg ggtcgtagtc gttttccagc acccattgca gtacgtcagt gtcgaggtaa
     1981 ttccactggt tacgcggagc cacaaacgcc ccgcgataac gtttcatttc aatcagccgc
     2041 ttcgccatca gcgaacgaaa tacctcacgg atgatgttgc gcgaggttgc aaactcctca
     2101 cagagttctg cctcagccgg aagcggcgag cctggcacgt atttgccgtg aacgatctgt
     2161 ctacccagcg taataacgat gcgatcggtt ttattgagag tcatggagag tccttgtgct
     2221 ctgtatgttc ttctctactt taccccgatc gatgcataac gcggcaattt tgtagtacca
     2281 gcgtgatgac gttcgcgttt gctgtgcgcc taatgtagta caaagttata ttgttgtact
     2341 acaatttaga tcacaaaaag aacaatccat aagaaaatgt tctacgtcgg gcaaaaatct
     2401 gaaaagggag atcaggcgct aaactggggg gaaagaagag tatgctttca acggcttagt
     2461 actcttgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttca aggctgccag
     2521 tttgtgttct ttggcatttg cgttgaccgg atgtatcacc tggggattgg tttccaatac
     2581 tgcaagccgt ccacatgccg agcaatggcg gtcagacacg attaagggtt tatcgctggc
     2641 agaagatagt aacggtacga aaggctatgt ttttgttggt gaatcgttgg actatctgtt
     2701 gaccaccggc ggcgatgagg tggttaaaat gctgaacgat ccggcaatcc atggtgaaag
     2761 aatcaccgtt tctgataacg cgaaatttat tttaagcagc agtaataaga atttctctgg
     2821 tgctattacg ctctattacg actggaacaa cgaagaagat aaggcactgg caacacaata
     2881 tggttttatt tgtgataccc ggcgttgtac atggatgctg gacggtttga aaggctcaat
     2941 ccaccagaaa aataaaaaag cggattactc aaacgtgatg gttttccatc aaccctttac
     3001 ggtgggattt tatgagtaca aagcaacaga tggcgtcccg cacggcctcg ttaatgcgct
     3061 gctgccagtc actcttacgc tggatatagt aacgtcacca ctccagttcc tgattttatg
     3121 cacgaccaga aattgttaat cgctgcgggc aaacgcattc atgccgtttg cccgttcact
     3181 gattaattca gcacatactt ctcaatagca aatgccacgc catcttcaag gttagatttg
     3241 gtgacaaagt tcgccacttc tttcaccgaa ggaatagcgt tatccatcgc cacaccgacg
     3301 cctgcatatt caatcattgc gatgtcgttt tcctgatcgc caatcgccat gatttcttcc
     3361 ggtttaatac ctaacacgtc ggccagtgat ttcacacccg tacctttgtt aacgcgttta
     3421 tcgaggattt cgaggaagta cggcgcactt ttcagcacgg tgtatttttc tttcacttcc
     3481 tgcggaatac gcgcaatggc ctggtcgagg atggcgggtt catcaatcat catcactttc
     3541 aggaactggg tattggggtc cattttctcc gcttcgcaga acaccagcgg aatggtggca
     3601 acgaaagatt catgcaccgt gtagtagctg atatcacgat tggcggtgta cagcgtggtg
     3661 cggtccagcg cgtggaaatg agaaccgact tcgcgagaga gtttttccag gaaacggtag
     3721 tcgtcatagc tgagagcggt ttgcgccacg gtgctaccat cagcggcctt ctgtaccagc
     3781 gcgccgttat aagtaatgca gtagtcgccc ggctgttcca tgtgcagctc tttcaggtaa
     3841 ttatgaacac ctgcataagg gcggcccgtc gtcagcacga catttacgcc acgggcgcga
     3901 gctgcggcaa tcgcattttt aacggcgggt gaaatggtgt tgtcgggcag cagaagggtg
     3961 ccatccatat cgatagcaat gagtttgata gccatgagtt ccccaggtag attggttcct
     4021 gacccatgct aacgcgattc cgctcaaaaa tcagtacaac accctgggga aaagggggat
     4081 gcaacgcgct ttgcgtgctc cccatttgct tagcggaaga gtttcccttt cagcagttcc
     4141 atgcctgcgg aaagcagatc gttattggct tgtggtgaca cttcaccttg cggtgagagt
     4201 gcatcaataa tcttcggcaa ttgttctgcc agtaaactgg aagctgtact ggtatcaacg
     4261 cccagttttt gcccgagatc ggacaccgca tttgtgccga gcgccgattc cagttgctcg
     4321 ccgctaaccg attgattgcc ctgttgatta ctcagccagg ttgagagaat ggcccctaag
     4381 ccgccacttt gcagtttttc cagcagcacc tgaataccgc cctgctcctc aacccagctc
     4441 aaaatagcct gatatttccc cgcatcgcct ttcagaaagg cgccgacaac ttcatcaaaa
     4501 aggcccatga taatcacctg taaagcgtta cgtgttgact caaaaagtat agttctgtgg
     4561 gatgataatt gcggattgca ggaataaaaa gggcggagat gatctccacc cttttcttat
     4621 agcttcttgc cggatgcggc gttaacgtct gatccggcct acaaaatcat gcaaattcaa
     4681 tatattgcag gagtctcgta ggcctgataa gcgtagtgca tcaggcacgc tcgcattgtt
     4741 aacgccatta aatatcgata ttcgccgctt tcagagcgtt ctcttcaata aacgcacggc
     4801 gcggttcaac ggcgtcgccc ataagcgtag tgaacaactg gtcggcagca atcgcatctt
     4861 taacggtaac gcgcagcata cgacggcttt ccgggtccat ggtggtttcc cacagctgtt
     4921 ccgggttcat ctcgcccaga cctttataac gctggatgga gaggccgcga cgggactctt
     4981 tcaccagcca gtccagcgcc tgctcgaagc tggctaccgg ctgacgacgt tcgccacgtt
     5041 cgataaatgc atcttcttcc agcaagccac gcagtttctc acccagcgtg cagatacgac
     5101 gatattcgcc gccagtgata aattcgtgat ccagcggata gtcagtatcc acaccgtggg
     5161 tacgcacgcg aacaatcggt tcaaacaggt tttgatcagc attggtgtgg acatcaaact
     5221 tccactggct gccgtgctgt tctttatcgt tcagttcgct gaccagcgcg ttcacccagc
     5281 gggtaacggt ttgctcgtca gaaaggtcgg tttccgtcag cgttggctga tagataagct
     5341 ctttcagcat tgctttcgga taacgacgct ccatgcggtt gatcattttc tgcgtcgcgt
     5401 tgtactcaga caccagtttc tctaacgctt cgccagccag cgccggtgca ctggcgttgg
     5461 tgtgcagcgt tgcgccatcc agcgcgatag agatctggta ctgatccatc gcttcgtcgt
     5521 ctttaatgta ctgttcctgc ttgcctttct tcactttgta cagcggcggc tgagcgatgt
     5581 agacgtgacc gcgttcaacg atttccggca tctgacgata gaagaaggtc aacagcagcg
     5641 tacgaatgtg cgagccgtcg acgtccgcat cggtcatgat gatgatgttg tgataacgca
     5701 gtttgtccgg gttgtactcg tcacgaccga taccacaacc aagcgcggtg ataagcgtcg
     5761 ccacttcctg agaagagagc atcttatcga agcgcgcttt ctcaacgttg aggattttac
     5821 ccttcagcgg cagaatcgcc tggttcttgc ggttacgccc ctgcttcgca gagccgcccg
     5881 cggagtcttc acgggcacgg gcagcatcga taattttgcc aaccacgatt ttcgcgtcgg
     5941 ttgggttttc cagcaggtat tccgccagca gttcgttcat ctgctgttca accgctgatt
     6001 tcacctcaga agaaaccagt ttgtctttgg tctgtgagga gaatttcggg tccggcactt
     6061 tcacggaaac gaccgcaatc aggccttcac gcgcatcgtc accagtggcg ctgactttgg
     6121 cttttttgct gtagccttct ttgtccatgt aggcgttcag agtacgggtc atcgccgcac
     6181 ggaaacctgc caggtgagta ccnnnnnnnn nngccgtcac gctgcggaat gttgttggta
     6241 aagcagtaga tgttttcctg gaagccatcg ttccactgca acgccacttc gacgccaata
     6301 ccgtcttttt cggtggaaaa gtagaagata ttcgggtgga tcggcgtttt gttcttgttc
     6361 agatattcaa cgaacgcctt gatgccgcct tcatagtgga agtggtcttc tttgccgtcg
     6421 cgcttgtcgc gcagacgaat ggaaacgccg gagttgagga acgacaactc acgcagacgt
     6481 ttcgccagaa tttcatattc gaactcggtc acattggtga aggtttcgag gctgggccag
     6541 aaacgcacca tggtgcctgt tttttcagtc tcgccggtaa ccgccagcgg agcctgcggt
     6601 acaccgtgtt cgtagatctg acggtgaatt ttaccctcgc gctggataac cagctccagt
     6661 ttttgcgaca gggcgtttac taccgaaaca ccaacgccgt gcagaccgcc ggacacttta
     6721 taggagttat cgtcaaattt accgcctgcg tgcagaacgg tcatgatcac ttccgccgcc
     6781 gatacgccct cttccgggtg aataccggtc ggaatgccgc gcccgtcatc ctgtacagag
     6841 acagagttat cggcgtgaat ggtgacgata atttctttac agtgacccgc gagcgcttcg
     6901 tcgatagcgt tatctaccac ctcgaatacc atgtggtgca gaccggtgcc gtcatccgtg
     6961 tcgccgatat acatacccgg gcgcttacgc accgcatcca gccctttcag gactttgata
     7021 ctggaggagt cataagaatt cgacatcaac gtttctcgct catttatact tgggttaatc
     7081 tgttatttta cccttttcca cggtaaacat cttcgaattt tcgtccgaca tgtctataac
     7141 gtgttcagca ctgatcgcgc tgacaaagac ctgtgattgc gtcgctttta agcggctggc
     7201 aagcagcccg cgacgctcat catcaagctc agaggcaaaa tcatctatca ggtagagaca
     7261 ccgccgcccg ctttcacggg tgaggaactc tccttgcgcc agacgtaagg cgcacatcag
     7321 cagcttaagc tgcccacgcg ataaggtatc ttccaccggc gcaccgtcgg cgcgaatgcg
     7381 taagtccgct ttatgcgggc catgcgcggt gtaggttagc tggcgatcgc gttcaaaatt
     7441 acgttccagc acctcagcat attctgtctc tttctcccag ccgcgctgga aagagaaagt
     7501 cagagaaaac tcagggagaa attgcttaca ggtatcggcc atatcagccg cgataccggc
     7561 gctatactcc gcgcgccagg tgctgatttg ctccgccagc gggatcagtt ctttatccca
     7621 cggacgtagc tgttcgtaac gagtcacctg gcgcagcgcc gcattgcgct gcttgagcaa
     7681 tcgcttgaga ttgctccagg cggtgaaaaa tccgggttcg ttgtgaaagc atccccagtc
     7741 gaggaatgct cttctgtatt tggggccgcc attgagtaaa gtaaaccctt ctggcgttat
     7801 cagctgcatt ggcatcaggt gcgccagctc cgcgacttta tgcccgtctg taccgtcaat
     7861 acggactttg ctatcgcctt gtttgtcttt ggttaaacca atcgccgtct cgcgctcttc
     7921 gccctgtaat cgcccgtgga gaacaaacgc ctcctgctcg tggcgaatga cgcgaccaat
     7981 ctgcaaactg cgaaacgccc gaccgtggcc gagcgtatag atggcttcca gcacgctggt
     8041 tttgccactg ccgttggcac ctaccagaaa gttaaagccg ggagataagg cgagatccgc
     8101 ggtttcaatg ttgcggaaat cgcggatcaa caagcgggtg agggacatta cagtctcann
     8161 nnnnnnnntg acaacataag ccgcgctctg gctggccgca tcttcaatct gcacgctgga
     8221 aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc agcgcgttga gaacatccag
     8281 cacatagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa cgtcgaggat
     8341 ctcttccgct tcttcctgtt ctgggttgtt ggcggtgatt ttcagctggt tttcgctgac
     8401 atacagacgc acgccgcgga atttctcgtt agagagaatt gccgcacggg caaacgcctg
     8461 cttgagcaga tcacagccag cttccagatg cttgtccggg ttcttcggca gaacgcgacg
     8521 gtaatccggg aagcgaccat ccaccagttt cgaggtgaag ataaagtcgc caacgtgggc
     8581 gcgaatgttg ttgctgccaa tctgcacgcg cagcggattg tcgccgccgt cgagcatacg
     8641 catcagttca atcacgcctt tacgcggcac gatcaccgaa tggcttggca aagattgacc
     8701 aattggcatt gaacagaccg ccagacggtg gccgtcggtc gccacggtgc gcagttcttc
     8761 accttcggtt tcaaacagca taccgtttaa gtaatagcga acgtcctgat gtgccataga
     8821 aaactgggtc gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa attcgacttc
     8881 gctctgccag tcatcgaggt tcgggaaatc tgccgctggc agggtagaca gcgaaaaacg
     8941 gctacgcccg gagcgcacca gcatccgctc accttccagc tgcacggcaa tttccgcgcc
     9001 ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg tcgcccctgg
     9061 ttcgtgcggc tgaaccagcg caacgcgtgc caccatttcc atctcgagat cagtaccggt
     9121 cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg gtagcgtagg
     9181 acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct cacgttctac
     9241 ggtaaatttc ataggtttac gatgacaatg ttctgattaa attggaaaaa tcttctttga
     9301 tatcgtggct ctcttcacgc agctgctcga tcttacggca ggcatgaagc accgtcgtgt
     9361 ggtcacggcc accaaacgca tcgccaatct ccggcagact gtggttagtc agttctttcg
     9421 ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc ttggaaagga
     9481 gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg tcgatggtga
     9541 ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag tcgatggtga
     9601 tcgcacgtcc ggtaaagttg gcgttagcaa taacgcggtt cagcgcgcct tccagctcac
     9661 gtacgttaga nnnnnnnnnn cacgccactt cgcccggcaa acgaatgtcg ttttcgtcgg
     9721 cctttttcat caggatcgcc acgcgggttt ccagctctgg cggttcgatc gccacagtca
     9781 gtccccagcc gaagcgggat ttcaaacgat cctcaacgcc gttgatctct ttcggatagc
     9841 gatcggaggt gagaatgatc tgttgattac cttccagcag ggcgttgaag gtgtggaaaa
     9901 actcttcctg agatcgttct ttattagcaa aaaactgaat atcgtcgatc agcagtgcat
     9961 ctacggaacg gtagtagcgt ttaaactctt cgatcgcgtt gttttgcaga gctttaacca
    10021 tgtcctgaac aaagcgctcg gagtgcatat aaaccacttt ggcattcggc ttgcgcgcca
    10081 taatgccgtt acccaccgca tgcagcaggt gagttttacc caaacccgtg ccgccataaa
    10141 ggaacaacgg gttataagca ccgccagggt tatctgccac ctggcgagcc gccgcgcgcg
    10201 ccagttggtt agatttacct tcaacgaagt tatcaaacgt gtgtttgacg tttacgttag
    10261 aacgataggt cggttctgcc ggagccggga cgttatccca acccgagcgc gtagaaggcg
    10321 cagcacgttg cggctgcgtt tgcgccacct gtgcaggggc cgcgacgttg ctcgtcactg
    10381 ccgcttgtgg cgtttgcgtc accggtttgg tgccgacttc aaaacgcagc tgtggggcat
    10441 ccgctccgca gaaactggtt agcagtccat tgatattatt aaggtacttg tcccgtaccc
    10501 aatcgaggac aaaacggttt ggcgcgtaca gggccagcgt gttatcgctc agttccgcct
    10561 gcaatgggcg tatccacata ctgaattctg tggctggtaa ctcatcctgc aatcgggcaa
    10621 gacactgctg ccaaagcgaa agtgacacgg cggactccac tcgaacaaaa gtcgataatg
    10681 actaaggctg aaacattcat gattgttgac gtacgtcgaa aagaccctgc atgagggtga
    10741 cgcacgaacc gctgtctgcg gttatatgcc cgatcaagat cctgcaaaac gatcgggacc
    10801 gcggatcata gcctaaactg cgcaagagat cttctgtttc tcacagattt tcccgattta
    10861 tccacaggat gatccagaac tcgctaagtg taaacgatcc tgccgcgggc cgggcacgat
    10921 ttacgccgca tattggaaaa tttaatgacc atagacaaaa attggcttaa tcgatctaat
    10981 aaagatccag gacgatcctt gcgctttacc cgtcagcccg tataatcctc cacccggcgc
    11041 gccatgctgg ttttcactgg tgtgaggccg tacgttttcc ccgcgaaaag gtgcggaaaa
    11101 gcgcggtaaa taaggaaaga gaattgactc cggagtgtac aattattaca atccggcctc
    11161 tttaatcacc catggcttcg gtgtccatcg tttcattttt cggcggatat ccaataaagc
    11221 cattgaattt attcaagttt aggtagaaat cgccatgaaa cgcacttttc aaccgtctgt
    11281 actgaagcgc aaccgttctc acggcttccg tgctcgtatg gctactaaaa atggtcgtca
    11341 ggttctggca cgtcgtcgtg ctaaaggccg cgctcgtctg accgtttcta agtaataaag
    11401 ctaacccctg agtggttaag ctcgcatttc ccagggagtt acgcttgtta actcccagtc
    11461 aattcacatt cgtcttccag cagccacaac gggctggcac gccgcaaatt acnnnnnnnn
    11521 nncggccgcc tgaattcgct ggggcatccc cgtatcggtc ttacagtcgc caagaaaaac
    11581 gttcgacgcg cccatgaacg caatcggatt aaacgtctga cgcgtgaaag cttccgtctg
    11641 cgccaacatg aactcccggc tatggatttc gtggtggtgg cgaaaaaagg ggttgccgac
    11701 ctcgataacc gtgctctctc ggaagcgttg gaaaaattat ggcgccgcca ctgtcgcctg
    11761 gctcgcgggt cctgatagcc ctcattcggg tctatcaacg cctgattagt ccgctactcg
    11821 ggccgcattg tcgtttcact ccaacctgtt caagctacgg aattgaggca ttgcgcaggt
    11881 ttggagtgat aaaaggcagt tggttgacgg tgaaacgcgt attaaaatgc caccctttac
    11941 accctggtgg tgacgatccc gtcccgcccg gaccatttga taccagagaa cactaacgat
    12001 ggattcgcaa cgcaatcttt tagtcatcgc tttgctgttc gtgtctttca tgatctggca
    12061 agcctgggag caggataaaa acccgcaacc tcaggcccaa cagaccacgc agacaacgac
    12121 caccgcagcg ggtagcgccg ccgaccaggg cgtaccggcc agtggccagg ggaaactgat
    12181 ctcggttaag accgacgtgc ttgatctgac catcaacacc cgtggtggtg atgttgagca
    12241 agctctgctg cctgcttacc cgaaagagct gaactctacc cagccgttcc agctgctgga
    12301 aacttcaccg cagtttattt atcaggcaca gagcggtctg accggtcgtg atggcccgga
    12361 taacccggct aacggcccgc gtccgctgta taacgttgaa aaagacgctt atgtgctggc
    12421 tgaaggtcaa aacgaactgc aggtgccgat gacgtatacc gacgcggcag gcaacacgtt
    12481 taccaaaacg tttgtcctga aacgtggtga ttacgctgtc aacgtcaact acaacgtgca
    12541 gaacgctggc gagaaaccgc tggaaatctc cacctttggt cagttgaagc aatccatcac
    12601 tctgccaccg catctcgata ccggaagcag caacttcgca ctgcacacct tccgtggtgc
    12661 ggcgtactcc acgcctgacg agaagtacga gaaatacaag ttcgatacca ttgccgataa
    12721 cgaaaacctg aacatctctt cgaaaggtgg ttgggtggca atgctgcaac agtatttcgc
    12781 gacggcgtgg atcccgcata acgacggtac caacaacttc tataccgcta atctgggtaa
    12841 cggcatcgcc gctatcggct ataaatctca gccggtactg gttcagcctg gtcagactgg
    12901 cgcgatgaac agcaccctgt gggttggccc ggaaattcag gacaaaatgg cagctgttgc
    12961 tccgcacctg gatctgaccg ttgattacgg ttggttgtgg ttcatctctc agccactgtt
    13021 caaactgctg aaatggatcc atagctttgt gggtaactgg ggcttctcca ttatcatcat
    13081 cacctttatc gttcgtggca tcatgtaccc gctgaccaaa gcgcagtaca cctccatggc
    13141 gaagatgcgt atgctgcagc cgaagattca ggcaatgcgt gagcgtctgg gcgatgacaa
    13201 acagcgcatc agccaggaaa tgatggcgct gtacaaagct gagaaggtta acccgctggg
    13261 cggctgcttc ccgctgctga tccagatgcc aatcttcctg gcgttgtact acatgctgat
    13321 gggttccgtt gaactgcgtc aggcaccgtt tgcactgtgg atccacgacc tgtctgcaca
    13381 ggacccgtac tacatcctgc cgatcctgat gggcgtaacg atgttcttca ttcagaagat
    13441 gtcgccgacc actgtgaccg acccgatgca gcagaagatc atgaccttta tgccggtcat
    13501 cttcactgtg ttcttcctgt ggttcccgtc aggtctggtg ctgtactata tcgtcagcaa
    13561 cctggtaacc attattcagc agcagctgat ttaccgtggt ctggaaaaac gtggcttgca
    13621 tagccgcgag aagaaaaaat cctgattcgg tgagttttcg ctaaaataag ggcggtcagt
    13681 tgaccgcctt ttttcttttc gtaggacgga taagcaccgc acatccgcca cacaaagcaa
    13741 caggaacatc atgagcgata atgacactat cgtagcccag gccacgcctc cgggacgtgg
    13801 cggcgttggc atcctgcgca tctccggcct caaagcccgt gaagttgccg aaaccgtgct
    13861 gggtaaactg cctaagccac gctacgccga ttatcttccg tttaaagacg ccgatggcag
    13921 cgtgctcgat caggggattg cgctatggtt ccctggcccg aactcgttca ccggcgaaga
    13981 tgtgctggaa ctgcaaggtc atggcggtcc ggtgatcctc gacctgctgt taaaacgcat
    14041 tctgaccatt cccggcctgc ggattgcccg ccctggtgag ttttccgaac gcgcatttct
    14101 gaacgataaa ctcgacttag cccaggccga ggcgattgcg gatcttatcg acgccagctc
    14161 ggaacaggcg gcccgttcgg cgctaaactc gctgcaaggc gcgttctccg cacgggtcaa
    14221 ccatctggtg gaagccctca cccacttgcg catttacgtc gaagcggcga ttgatttccc
    14281 ggatgaagag atcgatttcc tctccgacgg caaaattgaa gcccagctca ataacgtaat
    14341 tgccgatctc gacgcggtac gcgccgaagc tcgtcagggt agtttgttgc gcgaagggat
    14401 gaaagtggtg attgccggac gtcctaacgc cggtaaatcg agcctgctga acgcgctggc
    14461 gggccgtgaa gcagcaatcg taaccgatat cgccggaacc acgcgtgacg tgctgcgtga
    14521 gcatatccac attgacggaa tgccgttgca tatcatcgat accgccgggc tacgtgaagc
    14581 cagtgacgaa gttgaacgta ttggtatcga gcgcgcatgg caggaaattg aacaggcaga
    14641 ccgcgtgctg tttatggtcg atggcaccac gacagacgcc gttgatccgg cagagatctg
    14701 gccggaattt atcgcccgtc tgccagcgaa actgccgatc accgtggtgc gcaataaagc
    14761 cgatatcacc ggcgaaacgt tggggatgag cgaagtgaac ggtcacgcgt taattcgtct
    14821 ttcggcgcgg actggcgaag gcgtggatgt gctgcgtaac catctcaaac agagcatggg
    14881 ctttgacacc aatatggaag gcggcttcct ggcgcgtcgt cgtcacctac aggcgctgga
    14941 acaggcggca gagcatttgc agcaaggtaa agcgcaactg ttaggtgcct gggcgggtga
    15001 actgctggcg gaagagttgc gcctggcgca gcagaactta agcgagatca ccggggaatt
    15061 tacttcagac gacctactgg ggcggatttt ctccagcttc tgtatcggta agtaaccgcg
    15121 cttgcgaagc cgcattctga ctgtcagatg cggcttcgct tcattgttag cactcctgtt
    15181 attcctcaac ccttttttaa acattaaaat tcttacgtaa tttataatct ttaaaataga
    15241 tatttaatat tgcgtcccga acgattgtga ttcgattcac atttaaacaa tttcagaata
    15301 gacaaatttc ttgagtatta tactgtagcc tcgtgtcttt cgaggataag tgcattatga
    15361 atatcttaca tatatgtgtg acctcaaaat ggtttaatat tgacaacaag attgtcgatc
    15421 accgcccttg atttgccctt ctgtagccat caccagagcc aaaccgatta gattcaatgt
    15481 gatctatttg tttgctatat cttaattttg ccttttacaa aggccatctc tcgtttattt
    15541 acttgtttta gtaaatgatg gtgcttgcat atatatctgg cgaattaatg gatactgcaa
    15601 atgtaatatt cacagggatc actgtaatta aaataaatga aggattatgt aatggaaaac
    15661 tttaaacatc tccctgaacc gttccgcatt cgtgttattg agccagtaaa acgtaccact
    15721 cgcgcttatc gtgaagaggc aattattaaa tccggtatga acccgttcct gctggatagc
    15781 gaagatgtgt tnnnnnnnnn ngggcgtgga cgcagggtat gcaggccgcg atgatgcgcg
    15841 gcgacgaagc ttacagcggc agccgcagct actatgcgtt agccgagtca gtgaaaaata
    15901 tctttggtta tcaatacact attccgactc accagggccg tggtgcagag caaatctata
    15961 ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc aaaatggtgg
    16021 cgttctctaa ctatttcttt gataccacgc agggccatag ccagattaac ggctgtaccg
    16081 tgcgtaacgt ctatatcaaa gaagccttcg atactggcgt gcgttacgac tttaaaggca
    16141 actttgacct tgagggatta gaacgcggta ttgaagaagt tggcccgaat aacgtgccgt
    16201 atatcgttgc aaccatcacc agtaactccg caggtggtca gccggtttca ctggcaaact
    16261 taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtcatg gactccgcac
    16321 gctttgccga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa gactggacca
    16381 tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg tccgccaaga
    16441 aagatgcgat ggtgccgatg ggcggcctgc tgtgcatgaa agacgacagc ttctttgatg
    16501 tgtataccga atgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca tatggcggcc
    16561 tggaaggcgg cgctatggag cgtctggcgg taggtctgta tgacggcatg aatctggact
    16621 ggctggctta tcgtatcgcg caggtgcagt atctggtcga tggtctggaa gagattggcg
    16681 ttgtctgtca gcaggcgggc ggtcacgcgg cattcgttga tgcaggtaaa ctgttgccgc
    16741 atatcccggc agatcagttc ccggcacagg cgctggcctg cgagctgtat aaagtcgccg
    16801 gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa accggtaaac
    16861 aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa
    16921 cacatatgga cttcattatt gaagccttta aacatgtgaa agagaacgcg gcgaatatta
    16981 aagggttaac ctttacctac gaaccgaaag tattgcgtca cttcaccgca aaactgaaag
    17041 aagtttaatt aaaacactca gagtggctat aaggatgtta gccactctat taccctacat
    17101 cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca atcagaaaaa
    17161 aaacactctg cattttgggg tgttatggtt atagccggta cagtaattgg cggtgggatg
    17221 tttgctttac ctgttgacct tgccggagcc tggttttttt ggggcgcttt tattcttatt
    17281 atcgcctggt tttcaatgtt acattcaggg ctattattat tagaagcaaa cttaaattat
    17341 cccgtcggat ccagttttaa caccatcacc aaagatttaa tcggtaacac ctggaacatt
    17401 atcagcggta ttaccgttgc cttcgttctc tatattctca cttatgccta tatctctgct
    17461 aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc taatccacgt
    17521 attgtcggga tctgcactgc cattttcgtt gccagcgtat tgtggataag ttcgttagcc
    17581 gccagtcgta ttacctcatt gttccttggg ctgaagatta tctcctttgt aattgttttt
    17641 ggttctttct tcttccaggt cgattactcc attctgcgcg attccaccag caccactgcg
    17701 ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc gtcatttggt
    17761 ttccacggca atattcccag cctgattatt tgctatggca aaaagaaaga caaactaatc
    17821 aaaagcgtgg ttttcggttc gctgctggcg ctggtgattt atctcttctg gctctattgc
    17881 acgatgggga atatcccgcg agaaagcttt aaggcgataa tctcctcagg cggcaacgtt
    17941 gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga gttttgcctg
    18001 ctggtgttct ccaacttagc tgttgccagt tcgttctttg gtgtcacgct ggggttgttc
    18061 gattatctgg cggatctgtt taagattgat aactcccacg ccgggcgttt caaaaccgtg
    18121 ctgttaacct tcctgccacc tgctttgttg tatctgatct tcccgaacgg cttcatttac
    18181 gggatcggcg gtgccggact gtgtgccacc atctgggcgg taataattcc tgctgtactg
    18241 gcaatcaaag cccgcaagaa gtttcccaat cagatgttca cggtctgggg cggcaatctt
    18301 attccggcga ttgtcattct ctttggcata accgtaattt tatgctggtt cggtaacgtc
    18361 tttaacgtgt tacctaaatt tggctaaatc cttcaggaag ccagccattc gctggcttct
    18421 tgcctgtcag gaaatcactt atgtccaaat ggcaactcgc ctgattctcc ttcaccacgt
    18481 atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt ttgatttgta
    18541 gttttgccct ggttttactt tatcccgcag ggattgatat gtacctcgtt ggtttaccgc
    18601 gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc tccgtttatc
    18661 tggcggggat ggcggctgcg atgttatttg ccggtaaagt ggccgatcgt tcagggagaa
    18721 agcctgtcgc catacccggc gcggcgctat ttattatcgc ctcggtgttc tgttcactgg
    18781 ctgaaaccag cgcgttattt cttgcagggc gatttctaca gggactgggc gcgggctgtt
    18841 gttacgtagt ggcgtttgcc attttgcgcg acacgctgga tgatcgacgt cgggctaaag
    18901 tgctgtcgtt actcaacggc attacctgca tcattccggt gttagcgcca gtgctcggac
    18961 atctgattat gcttaacttc ccatggcaga gtctgttctg gacgatggca acgatgggca
    19021 tcgcggtact gatgctgtct ttgtttattt taaaagaaac gcgccctgcg gcccccgcag
    19081 cttcggacaa accacgagaa aatagcgagt cactgcttaa ccggtttttc ctcagccgtg
    19141 ttgttatcac caccctcagc gtttcggtga tcctcacctt cgtcaacacg tcgccagtat
    19201 tgctgatgga aatcatgggt tttgagcgcg gagaatacgc caccattatg gcgttgaccg
    19261 ctggcgtcag catgaccgtt tcattctcca cgccgtttgc gctgggaatt tttaagccac
    19321 gtacgttgat gatcacctca caggtgttat tcctggcggc ggggatcacc cttgccgttt
    19381 caccttccca tgcggtttcg ctgtttggta tcacgctgat ttgcgccggt ttctcggtag
    19441 gttttggtgt ggcgatgagt caggcgttag ggccgttttc attacgcgcg ggcgtagcca
    19501 gctcgacctt aggtattgcg caagtttgcg gttcgtcact gtggatttgg ctggcggcgg
    19561 tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt agcatagtga
    19621 gcctgttgct gattatgttc gtcgcgcctg gacgcccggt tgccgctcat gaagaaatcc
    19681 atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc
    19741 gtgaccaaag cggcgaagcg gatgaacgtg accccttcgg cggtgagtaa gtcgctggca
    19801 aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg gctgtcgccc
    19861 acaccgctaa tggtcagcat ggagcaaaat ctggcggagt ggatgcaaat gagcaa
//
LOCUS       DAWDRC010000008        19455 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1519_length_19455_cov_2.449227, whole genome shotgun sequence.
ACCESSION   DAWDRC010000008 DAWDRC010000000
VERSION     DAWDRC010000008.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19455)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19455)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1519_length_19455_cov_2.449227"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2527..2536
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3720..3729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5105..5114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5918..5927
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6335..6344
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7608..7617
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9536..9545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10276..10285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12892..12901
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18298..18307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    18648..18657
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4422 a         4915 c         5251 g         4757 t
ORIGIN      
        1 gacactacgc cgccgaaaag gcgttttcgc tggccaacag gaatgccgca gctggcggca
       61 ctgttgctgg tgctgctggt tgatagcctg gtggccccac atttctggca ggtggtgctt
      121 caggatgggc gtttgttcgg tagccctatc gacattctta accgtgcggc ccccgttgcg
      181 ctactggcga ttggcatgac gctggttatc gccaccggtg ggattgatct ctccgtaggg
      241 gcggtgatgg ctatcgccgg agccacaacg gctgcgatga cggtcgcggg attcagcctg
      301 ccaattgttt tgttaagcgc cctgggcact ggcatcctgg cgggattgtg gaacggcata
      361 ctggtagcga tcctcaaaat tcagccgttt gtcgccaccc tgatcctgat ggtcgccggg
      421 cgcggcgtgg cgcaactgat cacctccgga cagatcgtca catttaactc accggatctc
      481 tcctggttcg gcagtggatc gctgttgttc ctgccaacgc cagtcattat tgcggtgctg
      541 acgcttctcc tgttctggct gttgacccgc aaaacggcac tggggatgtt tatcgaagcc
      601 gttggtatca acattcgggc ggcaaaaaat gccggggtaa acacgcggat catcgtcatg
      661 ctcacctacg tgttgagcgg gctatgtgcg gcgattgcgg gcattatcgt ggcggcggat
      721 attcgcggtg ccgatgccaa caatgccgga ttatggctgg agctggacgc cattcttgca
      781 gtggtgatgg gtggcggatc gctgatgggc gggcgcttta acctgctgct ttcggtggtg
      841 ggggcgctga ttattcaggg aatgaacacc ggaattttgc tttctggctt tccgccggag
      901 atgaaccagg tggtgaaagc ggtggtggtg ctttgcgtgc tgattgttca gtcgcaacgc
      961 tttatcagtc tgattaaagg ggtacgtaac cgtgataaaa cgtaatttgc cactgatgat
     1021 caccatcggc gtctttgtgt tgggttatat ctactgtctg acccagtttc ccggttttgc
     1081 ttccacaaga gtgatctgca atatcctgac cgataatgcc tttcttggga taatcgccgt
     1141 tggcatgacc tttgtgatcc tctccggtgg gatcgatctc tccgtcggtt cggtgatcgc
     1201 ctttaccggc gtgtttctgg caaaagtgat tggcgatttc ggcctctcgc cgctgctggc
     1261 gtttccgctg gtgttggtga tgggctgtgc cttcggcgca tttatgggac ttctgatcga
     1321 cgccctgaag atcccggcgt ttatcattac tttggccgga atgttctttt tgcgtggcgt
     1381 cagctatctc gtttcggaag agtcgatccc gataaaccat cctgtttatg acacgctctc
     1441 aagccttgcg tggaaaatcc ctggcggcgg tcgcttaagt gcgatgggac tgctgatgct
     1501 ggcggtggtg gttatcggca tattcctcgc gcatcgtacc cgttttggta atcaggtata
     1561 cgccattggc ggcaacgcaa cgtcggcaaa cctgatgggg atttctaccc gcagcaccac
     1621 tattcgcatt tatatgctct ccaccggact ggcaacgctg gcggggattg tcttctcgat
     1681 ttatacccag gccggatatg cgctggcggg cgtaggtgtg gaactggatg ctatcgcctc
     1741 agtggtaatt ggcggtacgc ttttgagtgg tggcgttgga acggtattag ggacgctttt
     1801 tggcgtggcg attcagggac tgattcagac ttacataaac tttgatggca cgctgagttc
     1861 ctggtggacg aaaatcgcca tcggcatttt gctgttcatt tttattgcct tacagcgtgg
     1921 attaacggtg ctgtgggaga atcgtcagag ttcgccagtg acaagagtca acattgcgca
     1981 gcgataaaaa cgcctctccg tgtggagagg cgcaggagat tacgcgtccg ggaattcacg
     2041 gataaagcgt tcgacatctt caaccatatg gtcgttgccg acaaagaacg aacggcgctg
     2101 gtgcagggtt tccgggataa tatccagaat acgctctttg ccatcgctcg ctttaccgcc
     2161 cgcttgttcc gccaggaatg ccatcgggtt gcactcatac agtaaacgta gtttaccgtc
     2221 cgggtggctg gcggtgcttg ggtagagata aataccgcct ttcagcaggt tacggtggaa
     2281 atccgcgacc agtgaaccga tataacgtga ggtatacggg cggttggtgg atttatcttc
     2341 ttcctggcag aatttaatgt acttcttcac cccgttcgga aacttaatgt agtttccttc
     2401 gttgatggag taggttttgc ctttctccgg gaagcgcatc cgttcctggc acaggcagaa
     2461 aacacccagc gaaggatcat aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag
     2521 catagtnnnn nnnnnnagca tagtagagga gccgtatacc acgtaacctg ccgcaacctg
     2581 tttgttacca ggctggagga aatcttcttc cgttaccggc gtgccaacag gcgtaacgcg
     2641 gcggtagatg gagaaaatgg taccgacaga gacgttaaca tcgatgttgg acgagccatc
     2701 cagtgggtcc atcagcacca cgtattttgc gtgttcacag ccttcaaaga cgacaatctc
     2761 atcttcttct tcagaggcaa tgcccgcaac gatatcgcgt gctttcagtg cggctttcag
     2821 tttctcatta gcgaacaggt cgagtttctg ctgaacctcg ccctgcacgt tctcagcacc
     2881 gctggcaccc aggatatcaa ccagtcctgc tttgttgata tcgcgatgga taatcttggc
     2941 gcccagtttt attgccgaca gcaaagcagt gagctcaccg gtagcatgag aaaactcgtg
     3001 ctgcttttcg acaataaatt cacctaacgt tttcataaaa ctttccctgc aatgtttatg
     3061 gagtaaagcg accgcaacaa tcttaacaaa taatcccaat gttgcgctca ggtgaatcgc
     3121 gccagcaaat tacggattat cctgaaatgc gtttctcact tgcccgacat atgcgtaaaa
     3181 tgagcgccag attaaaaaag gatagtgacg tatgcgcatt catattttag gaatttgtgg
     3241 cacgtttatg ggcggtctgg cgatgctggc gcgccagtta ggccatgaag taacgggttc
     3301 ggacgccaat gtgtatccgc cgatgagcac cttacttgag aagcaaggca ttgaactgat
     3361 tcagggttac gatgccagcc agctcgatcc acagccggat ctggtgatta ttggtaacgc
     3421 catgacccgt ggaaatccgt gtgtggaagc ggtactggaa aaaaacatcc cttatatgtc
     3481 aggtccgcag tggctgcacg attttgtgct gcgcgaccgc tgggtgctgg ccgttgccgg
     3541 tacacacggc aaaaccacca cggcgggaat ggcgacctgg attctggaac aatgcggtta
     3601 caaaccggga tttgtgatcg gcggtgtgcc ggggaacttt gaggtttcgg cgcgtctggg
     3661 cgaaagcgac ttctttgtta tcgaagcgga tgagtacgac tgcgccttct tcgacaaacn
     3721 nnnnnnnnnc tctaaatttg tccattactg cccgcgtacg ctgatcctca acaaccttga
     3781 gttcgatcac gccgatatct ttgacgacct gaaagcgatc cagaaacagt tccaccatct
     3841 ggtgcgtatc gttccggggc agggccgtat tatctggccg gaaaacgata tcaacctgaa
     3901 acagaccatg gcgatgggct gttggagcga gcaggagctg gtgggcgagc aaggtcactg
     3961 gcaggcgaaa aagctgacca ctgacgcttc tgaatgggaa gtcttgctgg atggcgaaaa
     4021 agtgggcgaa gtgaaatggt cgctggtagg cgaacataat atgcacaatg gcctgatggc
     4081 gattgcggcg gctcgccatg ttggtgtagc gccggcagat gccgctaacg cgctgggttc
     4141 gtttattaac gctcgtcgcc gtctggagtt gcgtggtgaa gcgaatggcg tcacggtata
     4201 tgacgatttt gcccatcacc cgacggcgat tctggcaacg cttgcggcgc tgcgtggcaa
     4261 agttggtggt acggcgcgca ttattgccgt gctggaaccg cgctcaaaca ccatgaaaat
     4321 ggggatctgc aaagacgatc tggcaccttc attaggtcgt gccgatgaag tcttcctgct
     4381 gcaaccggcg catattccgt ggcaggtggc agaagtggca gaagcctgcg ttcagcctgc
     4441 acactggagc ggcgatgtgg atacgctggc agatatggtg gtgaaaaccg ctcagccggg
     4501 cgatcacatt ctggtgatga gtaacggcgg ttttggtggt attcatcaga aactgctgga
     4561 tggtctggcg aagaaagcgg aagccgcgca gtaattcaca gcctcagcct gatagagtgt
     4621 gtgtaggccg gataaggcgt tcacgccgca tccggcattt gagcatagtg cctgatgcga
     4681 cgcttgatgc gtcttatcag gcctacaggt gcaccgcatc cggcaaacca tactcacatc
     4741 aacaacgaaa attacccttc gttttccgct aactcacgca gatactggaa aatctggcgc
     4801 gcggatttcg gcggcttatt cccttctttc tctttcttcg cgttacggat cagagtacgc
     4861 agttgctgac gatcggcatc gggccacaga ttcaatactt cagcgatggc gtcatcacct
     4921 tgctcgatca gacgatcgcg caggttttcc agtttatgga acaacaccac ttgctggttg
     4981 tggcggtttt tcagcttatc cagcgcctga cggatcggct caacatcgcg ctggcgcagc
     5041 atcttaccga tgagctgcaa ctggcggcgg cgaccttcca tcttaatacg ctgggcgagc
     5101 tcaannnnnn nnnnaatagc cgcgcgcaga tccgcatcta acgggatctt atccagcgcg
     5161 tttttcccca gatcaacaat ttctgcgccc aggcgtttta gctcctcggc atcgcgttta
     5221 atttcacttt tactgaccca gataatttca tcgtcttcgt cttcgatgtc atcaccggga
     5281 acgtcgtcga gccagtcttc gggctgctta gtcatctcag gctccttaaa aaaagaggct
     5341 aatgttacca gttaagatgc gcactgaaaa acggttctct gttagacttc agagaaactc
     5401 tctacattat ggcacttgca atgaaagtaa tctctcaagt tgaagcgcag cgcaagattc
     5461 tggaagaagc agtttcgact gcgctggagt tggcctcagg caaatcggac ggtgcagaag
     5521 ttgccgtcag caagaccacc ggcattagcg taagcacgcg ttatggtgaa gtggagaatg
     5581 ttgaattcaa tagcgatggc gcgctgggga tcaccgttta tcaccagaac cgtaaaggta
     5641 gcgcatcgtc taccgattta agcccgcagg ccattgcccg caccgtacag gcggcgctgg
     5701 atattgcccg ttatacctcg ccagatccgt atgcaggcgt ggcagacaaa gaactgctgg
     5761 cttttgacgc gccggatctt gacttgttcc accctgcgga cgtttctccg gatgaagcca
     5821 ttgaactggc ggcccgcgcg gaacaggcgg cattacaggc ggacaaacgc atcaccaata
     5881 ccgaaggcgg tagctttaac agccactacg gtgtcaannn nnnnnnnagc gcgattatgc
     5941 ctacaccatt gggcgtgcga tgagcgatct gcaaacgcca gagtgggttg gggccgactg
     6001 tgctcgccgt accttatcgc gcctgtcacc gcgtaaactt tctaccatga aagcgccggt
     6061 gatttttgcc aatgaagtgg caacagggtt gtttggtcat ttggtggggg cgatagcggg
     6121 tggtgcggtc taccgtaaat cgaccttctt gctggattcg ctgggtaaac aaattctgcc
     6181 ggactggctg actattgaag aacatccgca tctgctgaaa gggctggcat ctacaccatt
     6241 cgacagcgaa ggtgtgcgca ccgagcgccg cgatatcgtc aaagatggca tcctgactca
     6301 gtggctgctg accagctact cggcgcggaa actgnnnnnn nnnncggaaa ctggggctga
     6361 aaagcaccgg acatgcgggc ggtattcaca actggcggat tgccggacaa gggctaagct
     6421 tcgaacaaat gctcaaagag atgggcaccg ggctggtggt gacggagctg atgggccagg
     6481 gcgtgagtgc cattaccggt gattattccc gtggtgcagc gggcttctgg gtagagaatg
     6541 gcgagattca gtatccggtg agcgaaatca ccatcgctgg taatttaaaa gatatgtggc
     6601 gcaatattgt caccgtcggt aacgatattg aaacacgcag taatatacag tgtggttctg
     6661 tgctgttgcc ggagatgaaa atcgccgggc agtaaaaaac tggcgcgacc tgccgcgcta
     6721 taaactaaaa aattacacaa aatcattcgc actgcatcga gacggaaagt gaatgagagc
     6781 aggggagcgt actcgtagta cgtgactcgg gcgaatgagc gtagccgtcg atgaggcagt
     6841 gtgaaggatg aagtgtaaat aaaaaaggaa gtgagcaatg cgtaaaagcc tgttagctat
     6901 tcttgcagtc tcctcattgg tattcagttc ggcgtcgttt gccgctgatc tggaagacaa
     6961 tatggaaacc ctcaacgaca atttaaaagt ggttgaaaaa gccgataacg cggcgcaagt
     7021 caaagacgcg ttaacgaaga tgcgcgctgc ggcgctggat gcgcaaaaag cgacgccgcc
     7081 gaagcttgaa ggcaaatcac cggacagccc ggaaatgaaa gacttccgcc acggtttcga
     7141 cattctggtc ggtcagattg acgacgcgct gaagctggca aatgaaggta aagtaaaaga
     7201 agcgcaggct gccgcagagc aactgaaaac gacccgcaac gcctatcacc agaagtatcg
     7261 ttaattcttt atttccctgt tgcctgcact caggtaacag ggaacttatc atcgcaaatg
     7321 atgcaccacc tgattgctgc tgccgcgcca gatcagggaa gggtctttta aatcctgcac
     7381 aaatttgccg tcgaccagca cgttaatcaa atcaacaacc tgcatttgcg cagcgttgag
     7441 ttcgtcgagt ttatagcctg tccacaccca gatgtcttta cccggacact cggcgcggat
     7501 gcgttgtacc agtttcagaa tatccggcac gttttgcgga tgtagcggat cgccgccgga
     7561 gagggaaata ccctggcgtt tgatacgagt gtcattcaga tcgttaannn nnnnnnncgt
     7621 tgacgatgtc gacaggatag tactgatgat agttcattat ttcacctggc ttatcagtcc
     7681 gcgtgtaggc tgcatccggt aatcaacgcc tgatgcgacg ctgtcgcgtc ttatcaggcc
     7741 tacagccgtt gccaaatgta ggccggataa ggcgtttacg ccgcatccgg catttgctta
     7801 acacagagtg aagattaacc tatctgccca tttcccaaat gtttaacgcg gcgcttaact
     7861 tcttcctgct taccagcgtt aaacggacgt gcatccgggc tacctaaata tccgcacacg
     7921 cggcgagtca ccgacacacg ggaggtgtca tggttaccac atttcggaca agtgaagcct
     7981 ttgctggtgc actcaaattc gccggtaaag ccacactcgt agcattcatc gatcggtgtg
     8041 ttggtgccgt aatacggtac atgctgatag ctgtaatccc agacatcttc cagcgccttc
     8101 aggttgtgct gaatgtttgg atactcgcca tagcaaatga agccaccgtt cgccagtggc
     8161 gggtaaggcg cttcaaagtc gatcttgtcg tacgggttca ccttcttctc cacatcgagg
     8221 tggaaactgt tggtgtagta acctttatcg gtcacgcccg gcaccacgcc aaactcagcg
     8281 gtatcgagac ggcagaagcg gtcacacagg ttctcactcg gcgtactgta gaggctgaaa
     8341 ccgtagccag tttcttcttt ccactgatcc accgcctgac gcagacgctc aacaatcgca
     8401 atacctttcg cgcgcagctg ctcgttgtcg taaacatgct cgccgccgaa cagcgcgtta
     8461 atggtttcgt ggatgccgat gtaacccagc gaaatagacg cacgaccgtt tttgaagatt
     8521 tcagaaacgt cgtcgtcagc attcagacgc acgccacaag cgccttccat atagaggatc
     8581 ggggctacgc gcgctttcac gccttcgaga cgagcgatac gggtcatcag cgccttacgt
     8641 gccaacacca gacgttcatc cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc
     8701 agagcaatac gcggcaggtt caggctgatc acgccgaggt tgttacgacc atcgtggatc
     8761 tgctcgccgt tttcattttc ccacacgccg aggaagctgc ggcagcccat cggggtttta
     8821 aacgaaccgg tgactttcac tacctgatcg tagttcagga tatccggata catgcgcttg
     8881 cttgcgcact ccagcgccag ctgtttgatg tcgtagttcg gatcgccttt tttatggttc
     8941 aggccatcgc gaatcgcaaa caccagtttc gggaacaccg ccgttttacg gtttttaccg
     9001 aggcctgcga tacggttacg caggatcgat tcctgaatca ggcgcgattc ccagctggtg
     9061 cccagaccaa aaccaaaggt tacaaacggc gtttgaccgt tggcggtatg cagggtgttt
     9121 acttcgtact ccagcgactg gaaggcatcg tagcactctt tgatggtacg agagttagcg
     9181 tagccttcgg cgtccgggat gttccactct tctgcggtct tgcgatgttt gttgtagctg
     9241 gcggtgacaa acggtgccag cacttcatcg atacggttaa tggtggtgcc gccataaata
     9301 tggctggcga cctgagcaat aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc
     9361 ggcggttcaa tctcggcgtt ccccatttta aagccctggg tcagcatgcc tttcaggtcg
     9421 atcaacatgc agttgaacat cgggaagaac ggcgagtaat cgagatcgtg atagtgaata
     9481 tcgccacgct catgtgcctg caccacgtca cgcggcagca ggtgctgacg tgcatnnnnn
     9541 nnnnncaggt cgcgctgggt tggaatcacc ttgctgtctt tgttggcgtt ttcgttgagt
     9601 aacgaggagt tggtctgctc gaccagacca cggatctcct ggttcaggcg accgcgtttt
     9661 tcacgttcaa tgtcgcgatc gtgacggtac tcgatgtagg cacgagccag ttgtttgtat
     9721 ggccccgaca tcagctggtt ttcaactgcg gtctggatct cattgatatc cacttggttg
     9781 cggccctgca tctgctcgct gacaaccgca gcaacagtgg cgcaataatc ggcatcatcg
     9841 acttccgctg ctttagctgc acgcagaatc gcttctttga tgcgctctga tttaaacggc
     9901 actttgcagc cgtctcgttt catcacatgc ggtgtcatga tcgctccata tttttaagaa
     9961 caggttatcc acagaaattg ggaaaggcgt tttccggttt tttcgtttcg ttttccggtg
    10021 ctttccgcaa tcgccatccg cttatccaca acgccgcacc tttttcgtgg ggagctgtag
    10081 tagcaattat agtcgattaa tacaacatat tgggttggga cgcattttaa agtctatata
    10141 tagtgctttg catcaaggat gtttgaactt tttttgatgt agctcaaagt aaaaagcaga
    10201 gcgtacggat gacgggcgct acagcgatat gtaaattttt taatgaattt tttgattata
    10261 aaatcaacaa aaacannnnn nnnnnactga caggcaaaac cccggaatga caggcattac
    10321 cggggcttag cgaagattta cttttgtaac caccagacag cctcaaaagg ccgtaaagtc
    10381 atggcgcagg gttgtggtga ggcttcttcg tagttatgca tcacaagctg ccagttgccg
    10441 cgcatttgcc ctggttgcca gggttggatc tcacggctaa ggttggcaat gaccagcaag
    10501 gtttgcccct tccattcacg gcgatagcac cacaatacag ggctgtttgg cagcagatcc
    10561 tggtaatcgc cccatgtcag gacggcttct tgcttacgca gtgcgattaa cttttggtag
    10621 gtgtaaaaca ccgaggactc atcggccagc gctgcttcta cgttgatttc ctgatagtta
    10681 tcgcccaggc caatccacgg ttcgccagcc gtaaacccgg cattatcgcc gttggtccat
    10741 tgcatgggtg tgcgactgtt gtcacgggat ttactggcga ggattgtcaa taactcgtcg
    10801 gcatcacgcc catcgttgcg cagctcggca aacatattga ggctctccac gtcgcgatag
    10861 tcagtaatgc gcgtgaaatg tgggttggtc atgccaatct cttcgccctg gtagatatac
    10921 ggcgttccct gcatgccatg cagcaccatc gccagcattt ttgccgcagg cacgcggtat
    10981 tcaccttcat caccaaagcg agaaacaatg cgcggctgat cgtggttaca ccagaacaag
    11041 gcattccatg ccacgttgtg cattccttgt tgccagtggc ggaacaatgt tttcaacgcc
    11101 acaaagtcag gtttagccag cgtccatttt tcaccaccgg gataatcgac cttcaggtga
    11161 tgaaaattaa aggtcatcga caattcactg cctgtcagtg ccgcgtatcg ctggcaatgc
    11221 tcaaggctgg tggaggacat ttcacctacg gtcattaacc cgcgtggcgt aaacacatcg
    11281 cggttcatct cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtatagaa gcgacgccca
    11341 tcgccatcca ggtcttcagg gtaacgcggg tctttggaga tcagattcac cacatccagg
    11401 cgcaacccgt cgaccccacg atcggcccag aactcacaga cttttttcag ctctgcgcgt
    11461 accgctggat tctcccagtt gagatccgcc tgttctggtg caaagagatg caaatagtac
    11521 tgttcgcttt ctgcatgcca gcgccacgca ctaccaccga attttgaacg ccagttgttc
    11581 ggtggcgttt ctggttctcc atcgcgccag atataaaact ggcggtaagg gctttctttg
    11641 ttcagcgcct cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac catatcgaga
    11701 atgatacgaa tcccacgcga ttttgcctgt gtcaccagtt cgtcaaaatc gtccagcgtg
    11761 ccataggtgg gatcaatcgc cgtataattc gctacgtcgt aaccgttatc gacctgggga
    11821 gagacataaa agggggttag ccagatggca tcaacgccca gtttatgcag atagtccagg
    11881 cgttggataa cgccacgtaa atcgccggtg ccgctacccg tggtgtcctg aaaactcttt
    11941 ggataaatct ggtagataac gccgttttgc caccagtggg gaagatgagt cattacgtta
    12001 ttcctgcaaa tgcgaggggg cgcaattgcg ccccgaagaa aattaaacaa tgtccagcgt
    12061 gcccaggcgg tatttccgct gatagataaa cgaggtgagt acaatcggga tgatgatggc
    12121 gatagccatt gccagcgcaa acacctgcca gtagctcggt tgaatcgaga gaattcccgg
    12181 caggccgcct acgccgatgc cattcgccat aacgccgttc aggccgcata gcaatcctgc
    12241 cagaccagaa ccaatcatcg cgcacagcat cgggaagcga tatttcaggt tgatgccgta
    12301 cattgcaggc tcagtgaccc caagccaggc ggagatagcg gcaggcacgg agatctcgcg
    12361 ttcattgtgc ttgcggctgg aaatgataat acctatcacg gcggagccct gagcgatatt
    12421 cgacagtgca atcagcggcc acactggcgt gccacccatg ctttgaatca tctgcaaatc
    12481 aatagcaagc gtggtctggt gtacgccggt gatcaccagc ggggcgtaca ggaagccaaa
    12541 caatgccgcg ccaataggag caaagctgcc ggtcatcagg tgacgtaccg caaaggcaac
    12601 gccatcgcca atcatgcgac caaacggacc aatcagcgca tgggcgagga acaccgcgag
    12661 gatcagcgaa cagacgggca ccaccaccag atagaggtaa tccggcacga tgcgcttaag
    12721 acgagtttca ataacgccca gcgccagtcc ggctaacagt gccgggatga cctgcgcctg
    12781 atagcccact ttggcgatgc tgaacatgcc aaagtcccat acttccggca gctgctgccc
    12841 gagcagataa gcgttcatca gctgtggaga aaccagcgtt acgccaagca cnnnnnnnnn
    12901 nacgataccg aggatcggtg taccacccat ttttttcacc gctgaccagc aaatcccgac
    12961 cggcaggtag aagaagatcg cttcaccgat caaccacaga aaatcgtaga tcgtttgcag
    13021 ggaagggtac atttgcgcca gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt
    13081 gcgaaaaccg aggatcaaac caccactaat caacgcgggc agcaatggga agaagatctc
    13141 cgcgaaatga gagatcaact gctcatgcca tttcatattc tgccgggcgg ctttttttac
    13201 ctgctcttta tcaacctgcg cctgtccggt tgacgcaatc agtgcttgat agtaatcacc
    13261 cacgttggtg ccaatcacca cctgaaattg cccggcattg gtgaaacagc ctttcaccat
    13321 ggggagttgc tcaatttctt tcggtctggc attggccggt tggttgagga caaagcgcag
    13381 gcgagtaata cagtggctca ccgtcgcaat attgccgcgc ccgccgacca tttcaatcaa
    13441 ccgatcgata tccgtttggt ttattttgct catcataaag ccccatggca gatgacattt
    13501 ttggttggct gcagaatata ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaaca
    13561 cagcgaagat cacaatttat cgttcgggaa acgatcagga cagggtggcg gggatgatga
    13621 tttgttgcgg ttcgctgcgc ccggtgacct gcgcgatcaa ctggcaggcc gcctggcgtc
    13681 cagcttcggc gtaaccggga tctacggtga cgatctccgg atggaggaat ttcattaacg
    13741 gtgtattacc gacgctcgcc agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac
    13801 ttgcgccaag tgccagcgtg tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca
    13861 cttttgctac gttctcatag ccttgcttca tagcaagccc tggcagagcg gcaacgggat
    13921 gcagtttatg cgctttgcag aacgccaggt aggcttcgtg acgtcgctta ccggtcgtca
    13981 cgtcactgtg cggcacgccg agatagctga tattacgatg tccctggtca tacagccgtt
    14041 gcatcaggat cttgattgcc ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt
    14101 cacgcgccag cagaaccagc gatgactgcc agtgggctaa catttcttct gttatgccgg
    14161 taaaaccgaa cagcactacg ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg
    14221 caactaattg tggggaaaac tgactttcca tcatgattgg atcgtaacct tgttcataga
    14281 acgctggcag catggtttga acggcgagat tttctgacaa cgaatccaga cgggtaacaa
    14341 tgatggcgac cactttatcg ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa
    14401 atccatgctg attcatcact gcttcaacac gctcgcgaga gcgctcgctc acgccgcttt
    14461 cgttattcag cacccgggaa accgtagatt tccccacgcc gcttaagcgc gcgatgtctt
    14521 tgatggtcag ccgattttgc atcctgttgt cccataacgt gttgtttaat tatttgagcc
    14581 taactttacc tgtgcattca gcaatgggta aagtctggtt tatcgttggt ttagttgcca
    14641 gcaggtatta tatcgccata aatgctacga atattattgg attctcctta ttatttgcgg
    14701 cgcttttttc acttaccgga ggttatatgg aacctgatcc cacgcctctc cctcgacgga
    14761 gattaaaact tttccggtaa gcccgtcttt tcacggcgtt accggatgcg taaggccgtg
    14821 acgttttaac gtccctgctc agctttatta ccttcaggta aggcttcgcc acgcctgaag
    14881 acatttctgt actgtttcag acagtgcgga gggactcctt atgtttaaag aaatttttac
    14941 ccggctcatt cgccatttac cttcccgtct ggttcatcgt gatccattgc ctggcgcgca
    15001 gcagacagtg aatgcggcgg tcccgccttc tttaagtgcg cattgcctga aaatggcggt
    15061 gatgcccgaa gaagaattgt ggaaaacgtt cgacacccat ccggaagggt taaatctggc
    15121 ggaagtggaa tctgcccgcg aacaacatgg tgaaaataaa ttacccgcac aacaaccgtc
    15181 gccgtggtgg gtacatttat gggtctgcta tcgcaacccc tttaatattt tgctcaccat
    15241 tcttggtgct atttcttacg ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat
    15301 ggtcgctatt tctacgttgc tgaactttat tcaggaagct cgttccacca aagcggcaga
    15361 tgccctgaaa gcgatggtca gtaataccgc gacggtactg cgagtaatta acgacaaagg
    15421 cgaaaatggc tggctggaga tcccgataga ccagctggtg cccggcgata ttataaaact
    15481 ggcggcgggg gatatgatcc cggcagattt acgtatcttg caggcgcggg atctgtttgt
    15541 cgctcaggcg tcgttaaccg gtgagtctct gcccgtagaa aaagccgcta ccactcgcca
    15601 gccggagcac agcaatccgc tggagtgcga caccctgtgt tttatgggca ccaccgtggt
    15661 gagcggcacg gcacaagcaa tggtgattgc cacaggtgcc aatacctggt ttggtcaact
    15721 ggcgggtcgt gttagtgagc aggaaagcga gccgaatgcc tttcagcaag ggattagccg
    15781 cgtcagtatg ctgctgattc gctttatgct ggtgatggct ccggtggtgt tgcttatcaa
    15841 tggttacact aaaggcgact ggtgggaagc tgcactgttt gcgctttcgg tcgcggtagg
    15901 cttaacgcca gaaatgttgc cgatgattgt tacctcgacg ctggcgcgcg gcgcagtcaa
    15961 gttgtcgaaa cagaaagtga tcgtcaaaca tctggatgcc attcagaact ttggcgcaat
    16021 ggatattctg tgcactgata aaaccggcac cctgacacag gataaaattg tgctggagaa
    16081 tcataccgat atctccggta aaactagcga acgcgtgctg catagcgcgt ggttgaacag
    16141 ccattaccag accgggctta aaaacctgct tgataccgcg gtgctcgaag gtacggatga
    16201 agagtcagcg cgctcgctgg ccagccgttg gcaaaaaatt gatgagattc catttgattt
    16261 cgagcgtcgt cggatgtcgg tggttgtggc agaaaatacc gaacaccatc agttggtttg
    16321 caaaggcgca ttgcaggaaa tcctcaatgt gtgttcgcaa gtgcgtcaca atggcgagat
    16381 tgtaccgctc gatgacacca tgctgcgtaa gattaagcgg gttactgata cgctgaatcg
    16441 tcaggggctg cgcgtggttg cggtggcgac gaaatacctg ccagcgcgtg aaggagatta
    16501 ccagcgggcg gatgaatccg acctgatcct cgaaggatat attgctttcc ttgatccgcc
    16561 aaaagagaca accgctccgg cactgaaggc attaaaagcg agtggaatta ccgtaaaaat
    16621 ccttactggc gacagtgagt tagttgcggc gaaagtgtgc catgaagtgg ggctggacgc
    16681 gggagaggtg gtcattggta gtgatattga aacgctatct gacgacgaac tggcaaatct
    16741 cgcacagcgc accacgctgt ttgctcgtct gacgccgatg cataaagaac gcatcgtaac
    16801 gttgctgaag cgcgaagggc atgtggttgg ctttatgggc gatggtatta atgatgcgcc
    16861 cgccttacgc gctgcggata tcggcatttc tgtggacggc gcggtagata ttgcccgtga
    16921 agcggctgat atcatcctac tggaaaaaag cctgatggtg ctggaagagg gggttattga
    16981 gggtcgtcgc actttcgcca acatgctgaa atacatcaaa atgacggcga gctctaactt
    17041 cggtaatgtg ttcagcgtgc tggtggcgag tgctttcttg cccttcctgc cgatgttgcc
    17101 gctacacttg cttattcaaa atctgctgta cgatgtgtca caggtggcga tcccatttga
    17161 taacgtcgac gacgagcaaa ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg
    17221 ctttatggtc ttcttcggac cgatcagctc gattttcgat attctgacgt tttgcctgat
    17281 gtggtgggta ttccatgcca acacgccgga aacgcaaacg ctgttccagt cgggctggtt
    17341 tgtggtgggc ttactgtcgc aaacgctgat tgtgcatatg atccgcaccc gccgtgtgcc
    17401 gtttattcag agctgtgcat cgtggccgtt aatgatcatg accgtgatcg tgatgattgt
    17461 cgggatcgca ttgccgtttt caccgctggc cagttatctg caactgcagg cgctgccgtt
    17521 aagctatttc ccgtggctgg ttgcgattct ggcagggtat atgacgttaa cccagttggt
    17581 gaaaggtttc tatagccgtc gttacggttg gcagtaaaga ataaactggg catggtggcc
    17641 cggaatcaga ttgcagacaa agtgagcttt gttcatgccg gatgcggcgt aaccgcctaa
    17701 tccggtctac aaattcaata aattgcagga atcacgtagg cctgataagc gtagcgcatc
    17761 aggcgatttt gcttttgtca tcagacttaa tccgggcatg atagcccgga tttccatcaa
    17821 gattagcgac gaacagcgat cgcttcgatc tcaatcttca cgtctttcgg cagacgggct
    17881 acttcaacgc aagaacgtgc cgggaaggtg gcgttgtgtt cggtgaagaa ggcttcgtag
    17941 gtagcattaa cggttgcgaa gtcgttcaga tctttcacaa acacggtagt tttaacgatg
    18001 tcgcccactt tcaggccagc ggcttcgacg atcgctttta cgttatccag cgactgacgc
    18061 gcctgtgcag cgacgtctgc cgggacttcg cccgttttcg gatttaccgg gatctgaccg
    18121 gaggtgatga tcatattgcc cagatcaacg ccctgtacgt aaggaccgat agctgccggt
    18181 gcattttccg tcgcgatagt tttgctcatg atttctcctt tattacagcg gtaaaaagtc
    18241 tggctcatta tagggagcca gactttttac cgctgtaata aaggagaaat catgagcnnn
    18301 nnnnnnncaa ccgcaattaa ttggccagca ccacattatg agaaaactct ttttcacagt
    18361 atttgcattt gagcgcgata tcattggcgc gttttcgcac ggcaaagctg gatgaaaccg
    18421 gttcggcatg gctgatacag ttgctgttag ggcagaccag cacattgtcg atgcgctctg
    18481 gcaaactggg gcgcgatttg ccaaccactt catagttgtc gatacggtta accgtggctt
    18541 gcggtgcata taacgccagc tgatcaacct gatcctcact taaaaaggta ttttcgattt
    18601 tgatcagatc cttgcgcccc atctcgccag aaggcaggtt cagaccannn nnnnnnngat
    18661 gcgctgatcc gtttcggtca gcttgaacag actcaacagc ttaaaaccga tctgggcggg
    18721 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    18781 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    18841 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    18901 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    18961 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    19021 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    19081 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    19141 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa tggttgccgt cgaacttcgc
    19201 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    19261 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    19321 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cggaaaactc
    19381 ggtggccagg cgcgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    19441 gctgataacc gaaat
//
LOCUS       DAWDRC010000009        19436 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1522_length_19436_cov_2.681957, whole genome shotgun sequence.
ACCESSION   DAWDRC010000009 DAWDRC010000000
VERSION     DAWDRC010000009.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19436)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19436)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_19436_cov_2.681957"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4342..4351
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12271..12280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4633 a         4883 c         5061 g         4839 t
ORIGIN      
        1 agctggaacc gtatcgccct ctgtttattg aagagccggt gctggcggaa caagccgaat
       61 actacccgaa actggcggca caaacgcata ttccactggc ggcgggtgag cgcatgttct
      121 cacgcttcga ttttaaacgc gtgctggaag ctggcggtat ttcgattctg caacccgatc
      181 tttctcacgc aggtggtatt accgaatgct acaaaattgc cggaatggca gaagcctatg
      241 acgtgaccct tgcgccgcac tgtccgctcg gcccgattgc actggcggct tgcctgcata
      301 tcgactttgt ttcctataac gcggtacttc aggaacaaag tatgggcatt cattacaaca
      361 aaggcgcgga gttactcgac tttgtgaaaa acaaagagga cttcagcatg gtcggcggtt
      421 tctttaaacc attaacgaaa ccgggcttag gcgtggaaat cgacgaagct aaagttattg
      481 agttcagtaa aaatgccccg gactggcgta atccgctctg gcgtcatgaa gataacagcg
      541 tagcagagtg gtaattcctg ccacgtaagc ccctcatcgg gcactaaaac agcaatacaa
      601 aaatataacc ctctgtaaat tacagggcat ggtgagcggc ttcgctatgc ccaaaatctg
      661 gagaaagctc gctatggata ttcccgttaa tgcagcaaag ccggggcgtc ggcgttatct
      721 gacgctggtg atgatcttta ttacggtggt catttgttat gttgaccgcg ctaacctggc
      781 cgtggcttct gcccatattc aggaagagtt tggtattact aaagcgcaaa tgggctatgt
      841 attttcggcc ttcgcctggc tttatacgct atgtcagatc cccggtggtt ggtttttaga
      901 tcgcgtaggt tcacgcgtga cttattttat tgcgatattt ggctggtcag tggcgacgtt
      961 attccagggc tttgccacgg gcttaatgtc attaattggt ctgcgcgcga taaccggtat
     1021 tttcgaagcg cccgcgttcc cgaccaataa ccggatggta accagttggt tcccggaaca
     1081 tgaacgtgct tctgccgttg gtttttatac gtctggtcag tttgtcggtc tggcgtttct
     1141 gactccgctg ctgatctgga ttcaggagat gttgagctgg cactgggtgt tcattgtcac
     1201 cggtggtatc ggcattatct ggtcgctgat ttggtttaag gtttatcagc cgccgcgcct
     1261 gaccaaaggc atcagcaaag ctgaactgga ttacattcgt gatggcggtg gtctggtgga
     1321 tggtgatgcg ccggtgaaaa aagaggcgcg tcagccgtta acagccaaag actggaaact
     1381 ggtgttccat cgtaaactga tcggtgtcta tcttgggcaa tttgcggtgg cttctacact
     1441 gtggtttttc ttaacctggt tcccgaacta tttaacccag gaaaaaggaa tcacggcgct
     1501 gaaagcaggt tttatgacca cggtgccatt cctcgcggcg tttattggcg tcctgctctc
     1561 tggctgggtc gcggatctgc tggtacgtaa gggcttttca ctgggctttg cgcgtaaaac
     1621 gccgattatc tgcggcttgc tgatctccac ctgcattatg ggtgcgaact acaccaacga
     1681 tccgatgatg attatgtgcc tgatggcgct ggcattcttc ggcaacggtt ttgcttcgat
     1741 aacctggtcg ctggtctctt ctctggcacc gatgcgcctg attggtttaa ccggcggtgt
     1801 gtttaacttt gccggtggtc tgggcggcat caccgttccg ctggtggtgg ggtaccttgc
     1861 gcagggttac ggtttcgcgc ctgcactggt ttatatctcc gccgtcgcgt tgattggcgc
     1921 gctctcttac atcctgctgg tgggcgatgt gaagcgcgtt ggataatctt tcaactgtgg
     1981 aatatgcgcc actccactgc gcataatcca ctttcttaaa gccggtgcgg tcaggcatcg
     2041 gctttttact atcttgccga agagttttaa acgcagtttg cgagtggcgc gccagtaagc
     2101 ggtattgagc ttctctggca gcttcagtaa taccgaacgc agaatctctg cgctatccag
     2161 cgcatagcta atcccctcca gcgagctggc gctgataaat cccgccgctt cgccaatcag
     2221 aaaggcgttg tccttgccgc agacaaaatc ctgccagcgt gagggaaaca gcaccgtgca
     2281 tttttcgctt ttcaccgcct taccaaactg gaactgaaag gcactcattt tctctttcag
     2341 cgccgtgaaa cgcgtctgac cgtctttcat cggataggca ccgccaaaga taaaataacc
     2401 gtctttgctg atactccatg agtaacagtc agttatcgca ttatcaaaga tgcaggagta
     2461 gaacggtacc ggatgtttct ctgcgaacca ctgctggatg gcgacatatt tacgaatctg
     2521 atgatccggg tagagatgtc gccgcaccat cgagttggca ccatcggcac cgaccagata
     2581 gcgggcggta atatgctgct cccagccgtc tgcgcgaaaa atgccattta tcatcctcac
     2641 gccagatttt tcgacacagg ctgtcgtggt aaacctcaac gctggcgggg atcagcgatt
     2701 tcatccacaa gtcgaaagca tggcgattaa tattgatata gcttcgctgg tagttacgcg
     2761 tgagcgatgc ggcgacgtcg acggttttga cgctgaaaat ctgcgggttg gcgatgacat
     2821 cgacaggaag cgtcagtcca tcgcgaataa aagatcgctg cgcgtccggt gccagcagac
     2881 cgccacaagg tttgctgaaa ccttcagtac catgctggtg ctttttatcc agcgcgatca
     2941 cctgcatttt gcctgctaac tttcgcgcca acgccgaccc tgccgggccg aggccaataa
     3001 tcgccacgtc gaaatgttcc atcttttcac tcacattcat cacgatggca aaagactatt
     3061 gtctgaaagt gaagtccttg tgaagtatga tggcgggaac cgttttgtat caggaccgcg
     3121 ccatgaaaca gataaccttt gctccccgta atcacctgct caccaatacc aatacctgga
     3181 cgcccgacag ccagtggctg gtgtttgacg tgcggccttc tggggcgtcg tttaccggcg
     3241 agaccattga gcgtgtgaat atccataccg gcgaggtcga ggttatctat cgcgcgtcac
     3301 agggcgcata cgtcggcgtg gtgaccgttc atccgaagtc agagaagtat gtcttcatcc
     3361 acggcccgga aaatcctgat gaaacatggt attacgactt tcatcatcgg cgcggagtga
     3421 ttgttgaaag cggcaaggtg agcaatctcg atgcaatgga tattaccgcg ccatatacgc
     3481 caggagcgct acgtggcggc agccatgtac atgtctttag cccgaacggt gaaagggtga
     3541 gctttaccta caacgaccat gtaatgcatg aactcgatcc ggcgctggat ttgcgaaatg
     3601 tcggtgttgc tgcaccgttt gtcccggtca acgtacaaaa gcagcatccg cgtgaataca
     3661 gcggtagcca ctggtgcgtg ctggtaagta aaaccacgcc cacgccacag cctggcagtg
     3721 atgaaatcaa tcgtgcttat gaagaaggat gggtaggaaa tcacgcgctg gcgtttattg
     3781 gcgacacact ttcggcaaag ggcgagaaag tgccggagct gtttattgtc gagttgccga
     3841 aagatgaagc tggctggaaa gcggcaggtg atgtgccgtt aagtggaacg gaaacaaccc
     3901 tgcctgcgcc accgcgtggt gtcgtgcagc gacgtttaac ctttacgcac catcgggctt
     3961 atccgggatt agtcaacgtc ccgcgccact gggtgcgctg taatccgcag ggtacgcaaa
     4021 tcgcgttttt aatgcgtgat gataacggca ttgtgcaact gtggcttatc tcgccacagg
     4081 gcagcgagcc gcgccagtta acccataaca aaacggatat tcagtctgca tttaactggc
     4141 atccatcagg cgaatggctg ggatttgtgc tggagaatcg gattgcttgt gcccatgcgc
     4201 aaagcggcaa ggttgagtat ttaaccgaaa accacgccaa tccaccttct gcggacgccg
     4261 tggttttctc gccggatggt caatggttgg cgtggatgga ggacggtcag ttgtggatca
     4321 ccgaaactga ccgctagctc tnnnnnnnnn ngcattttgg cggggggaat gacgttattg
     4381 gtttcttgcg cgtcggcttc gcttttttca acgcgcgagc gcactgagct gtctttgcgg
     4441 aacacatccc acggtagcaa tattgtgtcc agtactgcgg taaatggcat gtcgagtatc
     4501 gccagcgatt ttgtgcccca gttggtttca tcgtcgccaa tcatggttgc gctggcgcgg
     4561 gtgccaggat acgttccttc tttccccccg gtatgggaca tcacgctgga gcatcctccg
     4621 agtaatgtca ttccgctgca tatcatgaac gctaacaaca catttcttat cataataaat
     4681 tcatctgttg atcgtgggtg ttggcctgat gagttatagc gatcccttgc tgaaaataac
     4741 atcatcatta cgtcgcactg tggcggctat cgcactttaa cgtttcgtgc tgccccctca
     4801 gtctatgcaa tagaccataa actgcaaaaa aaagtccgct gataaggctt gaaaagttca
     4861 tttccagacc catttttaca tcgtagccga tgaggacgcg cctgatgggt gttctggcta
     4921 cctgacctgt ccattgtgga aggtcttaga ttctcgctga tttcaggagc tattgattat
     4981 gcgtaacttt gatttatccc cgctttaccg ttctgctatt ggatttgacc gtttgtttaa
     5041 ccacttagaa aacaaccaga gccagagtaa tggcggctac cctccgtata acgttgaact
     5101 ggtagacgaa aaccattacc gcattgctat cgctgtggct ggttttgctg agagcgaact
     5161 ggaaattacc gcccaggata atctgctggt ggtgaaaggt gctcatgccg acgaacaaaa
     5221 agagcgcacc tatctgtacc agggcatcgc tgaacgcaac tttgaacgca aattccagtt
     5281 agctgagaac attcatgttc gtggtgctaa cctggtgaat ggtctgctgt atatcgatct
     5341 cgaacgcgtg attccggaag cgaaaaaacc gcgccgtatc gaaatcaact aattccctaa
     5401 ggccgcctgg cgcggcctga catttccatg ctcgccgtca gggagcatat gcgaatcttc
     5461 ggatttgcag gtacttactc gcttcttaga aggagaaatg actatgcgta acttcgattt
     5521 atccccactg atgcgtcaat ggatcggttt tgacaaactg gccaacgcac tgcaaaacgc
     5581 cggtgaaagc cagagcttcc cgccgtacaa cattgagaaa agcgacgata accactaccg
     5641 cattaccctt gcgctggcag gtttccgtca ggaagattta gagattcaac tggaaggtac
     5701 gcgcctgagc gtaaaaggca cgccggagca gccaaaagaa gagaaaaaat ggctgcatca
     5761 agggcttatg aatcagcctt ttagtctgag ctttacgctg gctgaaaata tggaagtctc
     5821 tggcgcaacc ttcgtaaacg gtttactgca tattgattta attcgtaatg agcctgaacc
     5881 catcgcagcg cagcgtatcg ctatcagcga acgtcccgcg ttaaatagct aactagctat
     5941 tctctttgcc ccgccattct ggtggggctt tttttttgtc ttttttcttc agcaatgcgt
     6001 taaatttcgc ctgtttaaga tatttcggca cgttatttcc cgtgataatg tgcgctataa
     6061 ggcaaatctg cttcaccgat tcagccataa tccgtagcaa ttaaaatgtt atttcaagag
     6121 aaatggacga tgagtgatat agcattaacg gtcagtattc tggctttggt ggcggtcgtc
     6181 gggttgttta tcggcaacgt caagtttcgc ggtgttggat tgggcattgg cggcgtgctg
     6241 ttcggtggga tcatcgtcgg ccattttgtt tctcaggcgg ggatgacatt aagtagcgat
     6301 atgctgcatg ttattcagga gtttggcctg atcctgttcg tttataccat cgggattcag
     6361 gtagggccgg gcttctttgc ctcattgcgc gtctccggat tacgcctcaa cctgtttgct
     6421 gttctgatcg tcatcatcgg tggtctggtt accgccatcc tgcataaact gtttgatatt
     6481 ccactgccgg tagtgctggg gattttttcc ggcgcggtca ccaatacgcc agcgctgggg
     6541 gcagggcagc agatcttgcg cgacctgggg acaccgatgg cgatggtcga tcaaatgggg
     6601 atgagctatg cgatggcgta tccatttggg atttgcggca ttttgttcac tatgtggatg
     6661 ctgcgggtta ttttccgcgt caatgttgag actgaggccc tgcagcacga gtccacgcga
     6721 accaacggcg gtgcgctaat caggacgatt aacatccgag ttgagaaacc taatttgcat
     6781 aatttagcca ttaaagatgt gccgattctc aatggcgata aagttatttg ctcacgattg
     6841 aaacgagaag aaaccctaaa agtgccttcg ccagaaaccg ttattcaact gggcgatttg
     6901 ctgcatctgg tggggcagcc tgcggattta cataatgcgc aactggtgat tggtcaggag
     6961 gtcgatacct cgctgtctac gaaaggcact gatttgcgcg tcgcgcgtgt ggtggtcacc
     7021 aatgaaaacg tgctcggtaa acgtattcgc gacctgcact ttaaagaacg ctatgacgtt
     7081 gttatctcgc gcctgaaccg tgctggtgtt gaattagttg caagtagcga tatcagcctg
     7141 cagttcggcg atatcctcaa cctggtgggg cgtccgtccg caattgatgc cgttgccaat
     7201 gtgctgggga atgcgcagca aaaactgcaa caggttcaga tgctgccagt gtttattggt
     7261 atcgggcttg gcgtattgtt aggttctatt cccgtctttg tgcctgggtt cccggcggcg
     7321 ttgaaactcg ggctggcggg cggtccgctg attatggcgt tgatcctcgg gcgtatcggc
     7381 agtatcggca agctgtactg gtttatgcca ccaagcgcta acctggcgct acgcgagtta
     7441 ggcattgtgc tctttctctc ggtggttggc ctgaaatccg gcggcgattt tatccataca
     7501 ctggtggatg gcgaagggct aagctggatt ggttatggtg ccctgatcac cgccgttccg
     7561 ctgattactg ttggcattct ggcgcggatg ttagccaaaa tgaattacct gaccatgtgc
     7621 gggatgctgg caggttccat gaccgatcct ccggcgctgg cgtttgctaa taatcttcat
     7681 ccaaccagcg gtgcggcggc gctctcttac gccactgtct atccgttagt gatgttcctg
     7741 cgcattatca caccccaatt actggcggtg ctcttctgga gtatcggtta actcttttcc
     7801 cggtggagtc gccgtaaatg gtactccgcc tggtatttct gaacatcatc gagtcattta
     7861 cacactcgcc attctggtta ttacctgtcc gtattcttca gcacagctga cggcgcataa
     7921 ccaaatttat gcttaaaggc tttgctgaaa tgaaaggagt cgaagaagtt gagcgtatca
     7981 gcgacttcgc caaccgagtt cccttgttgc tttaatagcg atagcgccag atccagacgc
     8041 gcatcgaggt aatattcttt cggcgtcttg ccggtatagc gtaaaaacag acgacgcaac
     8101 caggcctcgc tacaggggat cgtggcagcc atatcagcta cgctccagcg ttgttgtaga
     8161 ttggcatgta aagtggcaat taatttttca atttgccgcc gttgtggatc ttttttaccg
     8221 actgcgtgca taagacaaat ccactgataa ataattttcg tcagaaaagc gactgccaga
     8281 ttatttttta ttgcttctgg tgaagtaatt aactcagcaa cttcggtaag ttcctgatta
     8341 taaacttcgc cattataaat aacgctttgc tgaccgacgg gaatatccat catactggtg
     8401 ggggtaaatt ccatccagta ctgttcccag accaaacctt cacagtgata agagtgaata
     8461 tccattggct ttaaaaatat aatacagttg ccatgcagag ttatttgttc gtcattcttt
     8521 aaaagaattt tcccacatcc ttgtacggta tacaccgcaa cccatgagtt ggcgataagc
     8581 ggtttcttct tgtcccgcgg ccagatcacc cgataatgct cgtcggcgta ggtatgccag
     8641 agcgatatca gagaaattcc gctggagatc acattttcgt taatcaataa gggaatattg
     8701 tacggagttt cgttttttac atccgaactt tgcaattttc cattcatttt aatatccctc
     8761 cggcgaatta tgctcacaac ctgaacttaa cagaaaacaa tccgttcagg gatatgacgt
     8821 ttgtatttta agaatctgac tgcctgaccc gacgcatttt acctctccct atattcatgc
     8881 gtccgggaca taaacataaa taagggctat gagatgaatt cgttacaaat cttgagtttt
     8941 gtcggtttta cgctgctggt ggctgtcatc acctggtgga aggtccgtaa aacagatacc
     9001 ggatcgcaac aaggctattt tcttgccgga cgttcactaa aagcgccggt tattgccgct
     9061 tcgttaatgc taaccaacct ttccacggaa caactggtcg gcctttccgg gcaggcctac
     9121 aaaagtggca tgtcggtgat gggctgggaa gtcacctctg cagtgacgct gatcttcctc
     9181 gcgctaatct ttttaccgcg ctatctgaag cgcggcattg ccaccatccc cgattttctg
     9241 gaggaacgtt atgataaaac gacgcgtatt atcatcgact tctgcttcct cattgccacc
     9301 ggcgtctgct ttctgccgat tgttctctat tccggcgcgt tggcgctcaa cagcctgttt
     9361 catgtgggtg agtcgctaca actttccgat ggtgcggcta tctggttact ggtgattttg
     9421 cttggtctgg cgggaatttt gtatgcggtg atcggcggac tgcgcgcaat ggcagtggcg
     9481 gactccatta acggtattgg gctggttatt ggcgggttga tggtgccgtt atttggcctg
     9541 atcgcgatgg gcaagggcag ctttatgcag ggcattgagc aactcaccac cgttcacgcc
     9601 gagaaattaa actcagtcgg cggcccgacc gatcccttgc cgattggcgc ggcatttacc
     9661 ggtttgattc tggtgaacac cttttactgg tgtacaaatc agggcatcgt gcaacgcacg
     9721 ctggcgtcaa aaagcctggc ggaagggcaa aagggggcgc tgttaacggc ggtgctgaaa
     9781 atgctcgacc cgctggtact ggtgctgcca gggttgattg cgtttcatct gtatcaggat
     9841 ttaccgaaag ccgacatggc ctacccgacg ctggtcaata acgttctgcc ggtgccactg
     9901 gtgggcttct tcggtgcggt gttatttggt gcggtgatca gtaccttcaa cggctttctg
     9961 aatagcgcca gtacgttatt cagtatgggt atttaccggc gcatcattaa ccagaatgcc
    10021 gagccgcaac agctggtcac cgtcgggcgc aaatttggtt tctttatcgc cgtcgtttcg
    10081 gtgatggtcg cgccgtggat cgccaacgcg ccgcaggggc tgtatagctg gatgaaacag
    10141 ctcaacggta tttacaacgt gccgctggtt accatcatca ttatgggctt tttcttcccg
    10201 cgcattccgg cgctggcggc aaaagtggcg atggggattg gcataatcag ctacatcacc
    10261 atcaactatc tggtgaagtt cgacttccat ttcctctatg tgctggcctg tacgttctgc
    10321 atcaacgtgg tcgtgatgct gatgatcggt tttatcaaac cgcgcgccac gccgttcacc
    10381 ttcaaagatg cgtttgcggt ggacatgaaa ccgtggaaaa acgtcaagat tgcgtcaatt
    10441 ggtatcctgt tcgcgatgat tggcgtctat gccgggctgg ctgaattcgg tggctacggt
    10501 acgcgctggt tagcgatgat cagttatttc atcgccgccg tagtgattgt ctacctgatt
    10561 tttgacagct ggcggcatcg tcacgaccca gccgtcacct ttactcccga tgcgaaggat
    10621 agcctatgaa acgccccaat tttctgttca tcatgaccga tacccaggcc accaatatgg
    10681 tcggttgcta tagcggtaag ccgctgaata cgcaaaatat tgatagtctg gcggcagaag
    10741 gtattcgctt taattctgcc tacacctgtt caccggtttg tacacctgca cgggccggac
    10801 tattcaccgg tatctacgct aaccagtccg gcccgtggac caacaacgtc gcgccgggca
    10861 aaaacatttc caccatgggg cgctacttta aggatgcggg ctatcacacc tgctacatcg
    10921 gcaaatggca tctcgatgga catgactatt tcggcactgg tgagtgtccg ccggagtggg
    10981 acgctgatta ctggttcgat ggcgcgaact atcttagtga actgacggag aaagagatta
    11041 gcctgtggcg caatggccta aacagcgttg aggatttaca ggcgaaccat attgacgaaa
    11101 ccttcacctg ggcgcaccgc atcagcaatc gggcggtgga ttttctgcaa cagcccgcgc
    11161 gcgccgacga acctttcctg atggtgattt cgtatgatga gccgcatcac ccgttcacct
    11221 gtccggtgga gtatttagag aaatacactg atttttacta cgaactgggt gagaaagcag
    11281 aggatgacct ggcgaacaaa ccggaacatc accgcttatg ggcgcaggcg atgccatcgc
    11341 cagtcggtga tgacgggctt tatcaccatc cgctctattt tgcctgcaat gactttgttg
    11401 atgaccaaat cggacgggtc atcaatgcct taacgccaga gcaacgtgaa aatacgtggg
    11461 ttatttatac ctccgatcac ggcgaaatga tgggcgcaca taagctgatc agtaaaggag
    11521 cggcgatgta tgacgacatc acccgcattc cgctgatcat ccgttcgccg cagggggagc
    11581 ggcgacaggt cgatacgcca gtcagtcata ttgacttact gccgacaatg atggcgctgg
    11641 cagatattga aaaaccagag attctgccgg gggaaaatat ccttgccgta aaagaaccac
    11701 gtggcgtgtt ggtggaattt aatcgctacg agattgagca tgacagcttt ggcggtttta
    11761 ttccggtgcg ttgctgggta acggatgact ttaaactggt gctcaacctc ttcaccagtg
    11821 atgaacttta cgatcgccgt aatgacccaa atgaaatgca taacctgatc gatgatatcc
    11881 attttgccga cgttcgcagc aaaatgcatg acgccttatt ggattacatg gacaaaattc
    11941 gcgatccgtt ccgcagttat caatggaacc tgcgtccgtg gcgcaaagat gcacaaccgc
    12001 gctggatggg ggcgtttcgt ccacgcccac aggatggtta ttctccagtg gtacgcgact
    12061 atgacaccgg tttaccgaca caaggggtga aggtggaaga gaaaaaacag aagttctgat
    12121 gcattattgc tgttcctggc ctaatttttc ggcgaggaac agtttacaag gagcaacaat
    12181 gaaaatttcc cgcctcggag aagcgcccga ttaccgcttc tcgctggcta atgagcgtac
    12241 ttttctggcg tggatccgta cagcactagg nnnnnnnnnn aggatttctg gcggcggggg
    12301 ttgggcttga tcaacttgcg ccagatttcg ccacgcccgt catccgcgaa ctgctggcgt
    12361 tgttgttgtg tctgttttcc ggcgggttag cgatgtatgg ctacttgcgc tggttacgca
    12421 atgaaaaagc gatgcgcctg aaagaggacc tgccctatac caacagctta ttaatcatca
    12481 gtttaatatt gatggtcgtg gcagtgatcg tcatgggact ggtgttgtat gccggatagc
    12541 cgcaaagcca gacgcattgc cgacccaggg ctgcagccgg agcgtacatc actggcgtgg
    12601 tttcgtacca tgctgggcta cggcgcgttg atggcgttgg ctatcaaaca caactggcac
    12661 caggcgggca tgttattctg gatttcgatt ggcatcctcg ccatcgtggc gctgatcctc
    12721 tggcactaca cccgtaatcg caatttaatg gatgtcacga atagcgattt ttcccaattt
    12781 cacgtagtcc gtgacaaatt tttgatctcc ctcgcggtgt tatctctcgc aatactgttt
    12841 gctgtaacgc atatacatca acttatcgta tttattgaga gagtcgcatg acaggaagtc
    12901 aggtcattga tgctgaagag gacaggcata aactggttgt agagtataag gatgctctgc
    12961 aacctgccga tttttatcac aatttcaaac agctgggcat ccgctcagta cagcttattc
    13021 cccatcttga atttgatgac cggggcgatc tgacggccgc ttcggtgaca gcagagctgt
    13081 gggggaaatt tttaattgcc ctgttcgagt gttgggtacg ggcagatatc agtcgtatct
    13141 cgattgagct ttttgacgcc accttacaaa aatggtgtgg cagcgaaaac ccgcaatcgc
    13201 gccgtgactg ccaggcgtgc gactggcatc gcctgtgccc gcacgctcgg caggagacgc
    13261 cagacaacgt gctttgtgcg ggctatcagg cgttttattc ttactccgca ccgcatatgc
    13321 gggtgatgcg tgatctaata aagcagcatc gttctcccat ggagctgatg acgatgctgc
    13381 ggtgacgtgc gcttaaacgg gctgcccctg atgcgacatc cgcgtcgcca gcggcagcca
    13441 gcacagcacg atcaacaatc ccattaaggt catcaacaac cccaggctgc cctgaccggt
    13501 ttgcggcaac atcgcagaga gcgacgccag cacgccggaa ccaatgtttt gcagaccgcc
    13561 gaccagcgcg ccagccgtgc ccgccaggaa ggggaacggc tccatcgcgc cgctggtagc
    13621 cagtggaaac agcatcccgg caccgaaaaa gaacagcgcg gcgggaatga gcagcgtcca
    13681 gacattcatc acgccaaacc agtcggggat ccacatcagc aagccagcca gcaggcagca
    13741 gataaccgac tgccacatca acgttgagaa gcgtttatta ggacgtccgg caaaccatgc
    13801 cccaaaaaat gccgccggaa tcggcagaat aaacaaaata ctgaccgtca tactgctaag
    13861 ccctaatacc gcgcccatca gcacgccgga gcaggcttca aaggcggcaa tcccggccag
    13921 accgccaatc agcatcagca aataacagtt aaaaccgctg ttaccgaaaa gcgttttata
    13981 actggtaagc aggcgcgtgc gcggtgcgtc gaccggacgc gtttccggca tccagcgggc
    14041 catactgaag gtcacaccgg cacaaagtac caacaaaaag agataacagg cgcgccagtt
    14101 ccacatcgta tccagcagac cgccgattag cggtgcgagc aacggactga caagaattcc
    14161 catgtttaac aggctattag catggcgcaa ctgtgttcgt tcatataaat cacgcggcaa
    14221 agtacgcgcc attacgccgc caacgccggt gcccatcccc tgcatcgcgc tggcggcaat
    14281 caatactgtc aaactggagg tcgtgaccgc gaccagcgtt gccagcataa aaatggacat
    14341 tccgacgagg atcaccggtc ggcgacccac gcggtcggaa atcgggccat aaaacagctg
    14401 tgagacaccg taagtcagca gataagcgcc cattacgctc tgcaccgccc cttcacggac
    14461 attgagatcg cgcgccatat cggcaatagc tggaatataa atggtttgcg ccatctgacc
    14521 gacggccacg agtaatacca acatcaataa caaattgacg tttctatgcc ttttcattat
    14581 cacggatgct tttataaaaa taattagaat aagtcactac gcattcccat aaatgcggga
    14641 gaggaatcta tcacattgat gaatgttagc cagatatacg ccaggaatgg ggaattgttt
    14701 agcggcagaa tatgtaaaca aaagcggcaa taaatgttgc cggaaacagt gatggtgcgg
    14761 ttggtagggt aaggtgttta cgctgtattc gacatgaata aggcgcactc tgtcaacaat
    14821 ctgaccgtct ttcaggtgat gatgctttat tgcctgagtt tcagttcaaa atgtgatctg
    14881 ccacgtattt ttatccggaa gaaaaatttt ccattgtctc ccctgtaaag ctgtgcttgt
    14941 ataaatattg ttaaacacaa aaccaacaag gtccccaatg actacttcca tgttcaacgc
    15001 aaaactacta ccaactgcgc catccgccgc agtggtcgtc gtgcgtgtgg tggtggtcgt
    15061 cggcaatgcg ccgtagggac tggaacaaca cacgattcca aaaccccgcc ggcgcaaacc
    15121 gggcggggtt tttcgtttaa gcacctcccg gaaagtcggc ccagaagaaa aggactggag
    15181 catggcaagt tcgggcacaa catcgacgcg taagcgcttt accggcgcag aatttatcgt
    15241 tcatttcctg gaacagcagg gcattaagat tgtgacgggt attccgggcg gttctatcct
    15301 gcctgtttac gatgccttaa gccaaagcac gcaaatccgc catattctgg ctcgccatga
    15361 acagggcgcg gggtttatcg ctcagggaat ggcgcgcacc gacggtaaac cggcggtctg
    15421 tatggcctgt agcggaccgg gtgcgactaa cctggtgacc gccattgccg atgcgaggct
    15481 ggactccatc ccgctgattt gcatcactgg tcaggttccc gcctcgatga tcggcaccga
    15541 cgccttccag gaagtcgaca cctacggcat ctctatcccc atcaccaaac acaactatct
    15601 ggtcagacat atcgaagaac tcccgcaggt catgagcgat gccttccgca ttgcgcaatc
    15661 aggccgccct ggcccggtgt ggatagacat tcctaaggat gtgcaaacgg cagtttttga
    15721 gattgaaaca cagcccgcta tggcagaaaa agctgccgca cccgccttta gcgaagaaag
    15781 tattcgtgac gcggcggcga tgattaacgc cgccaaacgc ccggtgcttt atctgggtgg
    15841 tggtgtgatc aacgcgcctg cgcgagtgcg tgaactggcg gagaaagcac aactgcctac
    15901 caccatgact ttaatggcgc tgggtatgtt gccaaaagcg catccgctgt cgctgggtat
    15961 gctggggatg cacggtgtgc gcagcaccaa ctatattttg caggaggcgg atctgctgat
    16021 tgtactcggt gcgcgttttg atgaccgggc gattggcaaa accgagcagt tctgtccgaa
    16081 tgccaaaatt attcatgtcg atatcgaccg cgcagagctg ggcaaaatca agcaaccgca
    16141 tgtggcaatt caggcggatg ttgatgacgt gttggcgcag ttgatcccgc tggtggaagc
    16201 gcaaccgcgt gcagagtggc accagttggt ggcggatttg cagcgtgaat tcccgtgtcc
    16261 aatcccgaaa gcgtgcgatc cattaagcca ttacggcctg atcaacgccg ttgccgcctg
    16321 tgtcgatgac aatgcgatta tcaccaccga cgtgggtcag catcagatgt ggaccgcgca
    16381 agcctatccg ctcaatcgcc cacgccagtg gctgacctcc ggcgggctgg gcacgatggg
    16441 ttttggcctg cctgcggcga ttggcgcggc gctggcgaac ccggaccgca aagtgttgtg
    16501 cttctccggc gacggcagcc tgatgatgaa tattcaggag atggcgaccg ccagtgaaaa
    16561 tcagctggat gttaaaatca ttttgatgaa caacgaagcg ctggggctgg tgcatcagca
    16621 acagagtctg ttctacaagc agggcgtttt tgccgctacc tatccgggca aaatcaactt
    16681 tatgcagatt gccgccggat tcggcctcga aacctgtgat ttgaataacg aagccgatcc
    16741 gcaggctgca ttgcaggaaa tcatcaatcg ccctggcccg gcgctgatcc atgtgcgcat
    16801 tgatgccgaa gaaaaagtgt acccgatggt gccgccaggt gcggcgaata ctgaaatggt
    16861 gggggaataa gccatgcaaa acacaactca tgataacgtg attctggaac tcaccgttcg
    16921 caaccatccg ggtgtaatga cccacgtttg tggccttttt gcccgtcgcg cttttaacgt
    16981 tgaaggcatt ctctgcctgc cgattcagga cagcgacaaa agccacatct ggctactggt
    17041 caatgacgac cagcgtctgg agcagatgat aagccagata gacaagctgg aagatgtggt
    17101 gaaagtgcag cgtaatcagt ccgatccgac gatgtttaac aagattgcgg tgttttttca
    17161 gtaagtgatt ttctcgcctg gatgggatat ttacatccgg gcgagcttat atactctaaa
    17221 taattcgagt tgcaggaagg cgacaagcga gtgaatcccc aggagcttac ataagtaagt
    17281 gactggggtg agcgaacgca gacgcagcac atgcaacttg aagtatgacg agtatattta
    17341 cagcatgttc cagccgatca gcatattcca ccaccacacg ccgagggtga tatgcaccag
    17401 gaaggttaat atcgtcagta ccgcaccgac caaccaccag gatttaatat cgttgtaacc
    17461 cacgccaaag atgaccggac ctgccgcgcc gccatagtga gtaaccatgc cgccatagga
    17521 gttagagaac aacagtgcca gcgcggttaa cattaacggt gcgccggaga cattcgccag
    17581 catggcaaat accggtaaca tggcaacgat ataggcacta ccagaagcga agaaataacg
    17641 cacgatgatg ctgaggaaaa taataacgaa gaaagcaacg ttcccgtgac catcaaatgc
    17701 caggttattt ttaaagactt cagctaacca ttcgaagaat ttaactttcg ataataagga
    17761 gcttaagccg ataataccgc cgtaccagat taaggtattc cagccgcctt tatttttaac
    17821 cacgtcttcc caggtaacga tacccagcag cagcatagtt gccataacaa cgattgccac
    17881 ggtggattca tcaaccccca gagacttact gaaaatccag cccagcagcg ccagcacaaa
    17941 gacaccgagc agcatttttt cgcggatttt catcggtccc agttcggcaa ggcccgcttt
    18001 agcgatggtt ttgttatcca ccttcttaat ttctggtgga tacatggtgt aaatcaccag
    18061 cggggtgacc agcagcataa tgatgccagg caatccggcg gctagcgccc atccacccca
    18121 gctaatttgc aggtgcagaa tgtcgttgat cattttcagc gccagaatgt tccccgccat
    18181 tgcggtaaag aacatatagc tggtggtttt agtgaccatg taaatggaca tcatcaggta
    18241 atgtccaaca cgacgcggac ttttttccgg ttctgagccc aaagccactg ccacgctgtt
    18301 gatgatcggt aacacgatgc cgcccgcacg cgcggtgtta gacggtgttg ccggagccag
    18361 taccagatcg agaaataccg taacgtaacc cagacccagg gtagtgctac caattttacc
    18421 aatcagcaga taggcaatac gtttacctaa gcctgtggtt acaaacgcgg cgcttaaagt
    18481 aaacgcagaa aacaccagcc aggtggtacc ggaagagtaa ccgcttaata cggcggtggt
    18541 tttaaattcc ccacctgata agttacccac cactaccatc gatgcggcga cggcaattaa
    18601 cagtacgaca ggttccggga aaggcttgat aaccagcccc acaatggccg ccaggtaaat
    18661 accaaaaagc acccacgcca gttcgcttaa tcctgccgga gctggcataa agccaataat
    18721 acatgggatc gctaatatca gaattagctt ccataacgat tttttattca tttaatatcc
    18781 tgttctttct gtatgactca tacagaattc ctttattgat tgtcattatc acgacaacaa
    18841 aatttttcag ggaagataaa cttcaccgga aaatattttt cttgtcgtcc gaataacaga
    18901 tgcgcgtaac gtcgtggcat cctgaccttc attacttaaa tgaacgtcga gcgcaccact
    18961 ggggtgttca atattgatat tgccgtatcc tacagaaggg acgatttgtc gggtgacggt
    19021 gccttccaat gcacaactac tggaaatagc aatagcaccg gttatcgcca aagcgcgatg
    19081 gcaagaatgc ggcataaaat aacgcacatt aattgccccg cctttctgcg ctggagaaat
    19141 aagtacaggt ttagggataa ccatattact gacatcgcct aagcccattg ctttacccgc
    19201 ttgtagacgg atagattcaa tgcggactaa taatgctttg tcggcatcca gctctgccgg
    19261 taattcatag cctgtttttc ccagatattt agcaggaata atgacaactg gcatcgccat
    19321 atcgatacag gtcaccggga catcgtcaaa ataatcaatc tgattatccg tcgggaagac
    19381 ttttccggtt ttcgttccgg cggcattcag gaaagtgagc gcaaccggtg cggcag
//
LOCUS       DAWDRC010000010        19405 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1526_length_19405_cov_2.460568, whole genome shotgun sequence.
ACCESSION   DAWDRC010000010 DAWDRC010000000
VERSION     DAWDRC010000010.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19405)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 19405)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1526_length_19405_cov_2.460568"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1289..1298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4989..4998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5494..5503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6947..6956
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7566..7575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8213..8222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9422..9431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12311..12320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13986..13995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14425..14434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    16548..16557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17000..17009
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17249..17258
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4266 a         5084 c         5280 g         4645 t
ORIGIN      
        1 gcgccggtga ggccatggag cagggcggca caccgcccgc tcctgagatc gtcccttttt
       61 ctctggtggg ccgggtaatt tgcagtggct ctatcctaat gaactcttcc cgacggatat
      121 ccgcgcctct gccgtgggcg tgattatgtc cttaagccgt attggcacta ttgtatcgac
      181 ctggacactg ccgatcttta tcaataatta cggtatcagt aacacgatgc taatgggggc
      241 gggtatctcg ctgtttggct tgttgatttc cgtagcgttt gccccggaga ctcgagggat
      301 gtcactggcg cagaccagca atatgacgat ccgcgggcag agaatggggt aatttgttca
      361 gatttctctc ttttctgaat caatattatt gactataagc tgcgtgaata tatgactaca
      421 ctttgtggga aaacaaaggc gtaatcacgc gggctaccta tgattcttat aatttatgcg
      481 catccatatc cgcatcattc ccatgcgaat aaacggatgc ttgaacaggc aaggacgctg
      541 gaaggcgtcg aaattcgctc tctttatcaa ctctatcctg acttcaatat cgacattgcc
      601 gccgaacagg aggcgctgtc tcgtgccgat ctgatcgtct ggcagcaccc gatgcagtgg
      661 tacagcattc ctccgctcct caaactttgg atcgataaag ttttctcgca cggatgggct
      721 tacggtcacg gcggcacggc gctgcacggc aaacatttgc tgtgggcggt gacgaccggc
      781 ggcggggaaa gccattttga gattggtgcg catccgggct ttgatgtgct gtcgcagccg
      841 ctacaggcga cggcaatcta ctgcgggctg aactggctgc caccgtttgc catgcactgc
      901 acctttattt gtgacgacga aaccctcgaa gggcaggcgc ggcactataa gcaacgtctg
      961 ctggaatggc aggaggcaca tcatggatag ccatacgctg attcaggcgc tgatttatct
     1021 cggttcggcg gcgctgattg ttcccattgc ggtacgtctc ggtctgggat cggttcttgg
     1081 ctacctgatc gccggctgca ttattggccc gtgggggctg cgactggtga ccgatgccga
     1141 atctattctg cactttgcag agattggcgt ggtgctgatg ctgtttatta tcggcctcga
     1201 actcgatcca caaaggctgt ggaagctgcg tgcggcagtg ttcggcggtg gcgcattgca
     1261 gatggtgatt tgcggcggcc tgctggggnn nnnnnnnntg ttctgcatgt tacttgggct
     1321 gcgctggcag gtcgcggaat tgatcggcat gacgctggcg ctctcctcta cggcgattgc
     1381 catgcaggcg atgaatgaac gcaatctgat ggtgacgcaa atgggccgca gtgcctttgc
     1441 ggtgctgctg ttccaggata tcgcggcgat cccactggtg gcgatgatcc cgctactggc
     1501 agcgagcagt gcctcgacga cgatgggcgc atttgttctc tcggcgttaa aagtggcggg
     1561 cgcgctggtg ctggtggtat tgctggggcg ctatgtcacg cgtccggcac tgcgttttgt
     1621 tgcccgctct ggcttgcggg aagtgtttag tgccgtggcg ttattcctcg tgtttggctt
     1681 tggtttgctg ctggaagagg tcggcttgtc gatggcgatg ggcgcgtttc tggcgggcgt
     1741 actgctggca agctcggaat accgtcatgc gctggaaagc gatatcgaac catttaaagg
     1801 tttgctgctg gggctgtttt tcatcggcgt tggcatgtcg atagactttg gcacgctgct
     1861 tgaaaatccg ttgcgcattg ttattttgct gctcggcttc ctcatcatca aaatcgccat
     1921 gctgtggctg attgcccgac cgttgcaggt gccaaataaa cagcgtcgct ggtttgcggt
     1981 gctgttaggg cagggcagtg agtttgcctt tgtagtattt ggtgcggcgc agatggcgaa
     2041 tgtgctggag ccagagtggg cgaaatcgct gactctggcg gtggcactgt cgatggcggc
     2101 aacgccgatt ctgctggtga tcctcaatcg ccttgagcaa tcttctactg aagaggcgcg
     2161 tgaagccgat gagatcgacg aagaacagcc gcgggtaatc attgccggat tcgggcgttt
     2221 tgggcagata accggacgtt tactgctctc cagcggggtg aaaatggtgg tgctcgatca
     2281 cgacccggac catatcgaaa cgctgcgtaa attcggtatg aaagtgtttt atggtgatgc
     2341 cacgcggatg gatttactgg aatctgccgg agcggcgaaa gcggaagtgc tgattaacgc
     2401 catcgacgat ccgcaaacca acctgcaact gacagagatg gtgaaagaac atttcccaca
     2461 tttgcagatt attgcccgcg cccgcgatgt cgaccactac attcgtttgc gtcaggcagg
     2521 cgttgaaaag ccggagcgtg aaaccttcga aggtgcgctg aaaaccgggc gtctggcact
     2581 ggaaagttta ggtctggggc cgtatgaagc gcgagaacgt gccgatgtgt tccgccgctt
     2641 taatattcag atggtggaag agatggcaat ggttgagaac gacaccaaag cccgtgcggc
     2701 ggtctataaa cgcaccagcg cgatgttaag tgagatcatt accgaagacc gtgaacatct
     2761 gtcattaatt caacgacatg gctggcaggg aaccgaagaa gggaaacata ctggcaatat
     2821 ggcgaatgaa ccggaaacga agccctcatc ttaataaaga gtgacgtaaa tcacgcttta
     2881 cagctaactg tttgtttttg tttcattgta atgcggcgag tccagggaga gagcgtggac
     2941 tcgccagcag aatataaaat tttcctcaac atcatcctcg caccagtcga cgacggttta
     3001 cgctttacgt atagtggcga caattttttt tatcgggaaa tctcaatgat cagtctgatt
     3061 gcggcgttag cggtagatcg cgttatcggc atggaaaacg ccatgccgtg gaacctgcct
     3121 gccgatctcg cctggtttaa acgcaacact ttaaataaac ccgtgattat gggccgccat
     3181 acctgggaat ccatcggtcg tccgttgcca ggacgcaaaa atattatcct cagcagtcaa
     3241 ccgggtacgg acgatcgcgt aacgtgggtg aagtcggtgg atgaagccat tgcggcgtgt
     3301 ggtgacgtac cagaaatcat ggtgattggc ggtggtcgcg tttatgagca gttcctgcca
     3361 aaagcgcaaa aactgtatct gacgcatatt gacgcagaag tggaaggcga cacccatttc
     3421 ccggattacg agccggatga ctgggaatcg gtattcagcg aattccacga cgctgatgcg
     3481 cagaactctc atagctattg ctttgagatt ctggagcggc ggtaagtttg tatattagaa
     3541 ttccggcatc actgatacgt gcagatatcc gtgtatacta gacgtataaa ttgtacagga
     3601 gcacgatatc atgactgcaa aacgtaccac acaaagtgtg accgtcaccg tcgaccgtga
     3661 gttagtcaat cgcgctcgtg atgcaggctt aaatatgagc gccaccctta cggttgcgct
     3721 caatgctgaa cttaaaaaac atgcagcaac acgttggcgt gaagagaacg cagaagctat
     3781 cgctgcgtta aatcaattgg ctgatgaaac cggatgtttc tctgatgagt accggagctt
     3841 ctagcgatgc aatttacggt ataccgcagt cgcggcagga acgccgcgtt tccctttgtt
     3901 attgatgtta ccagcgacat tgttggtgag attaatcgcc gtatcgttat tccattaaca
     3961 cctattgaac gatttagccg tattcgccca ccagaacggc ttaacccaat ccttttactg
     4021 gtagacggca aagaatatgt gctcatgacg catgagactg caactgttcc agttaacgcg
     4081 ctgggaacga aattttgcga tgccagcgcg caccgaacgt tgattaaagg agcattagat
     4141 tttatgctcg acgggattta agacgctacc gcctctccct cacccatatc tttatgccgg
     4201 ttcgacggct ggacaaaata ctgtttatct tcccagcgca ggcaggttaa tgtaccaccc
     4261 cagcagcagc cggtatccag cgcgtaaata ccttccggcg tacctttgcc ttccagcgat
     4321 gcccagtgac caaaggcgat gttgtactct tcagcgacgg ggccaggaat cgcaaaccac
     4381 ggtttcagtg gggcaggggc ctcttccggc gattctttgc tgtacatatc cagttgaccg
     4441 ttcgggaagc aaaaacgcat acgggtaaag gcgttggtga taaaacgcag tcttcccagc
     4501 ccccgcaatt ccggtgacca gttatttggc atatcgccgt acatggcatc aaggaagaag
     4561 ggataggagt cactcgacag caccgcttct acatcgcgcg cgcactcttt ggcggtctgc
     4621 agatcccact gcggcgtgat cccggcgtgg gccatcacca actttttctc ttcgtcgatt
     4681 tgcagcaaag gctggcgacg cagccagtta agcagctcgt cggcatccgg cgcttccagc
     4741 agcggtgtca ggcgatcttt cggtttattg cggctgatcc cggcaaatac cgccagcaga
     4801 tgcagatcgt gattacccag caccagacgt acgctgtcgc ctaaggattt cacatagcgc
     4861 agaacatcca gcgaacccgg gccgcgcgcg accagatcgc ccgtcagcca gagggtatct
     4921 ttcccagggg taaattctac tttatgcagc aatgcgatca gttcatcgta acaaccatga
     4981 acgtcgccnn nnnnnnnnat gagtgtggga acggcgagtc ggaatacggg aatgtcgatg
     5041 ctgaaaggga cgccattttc atcgatcatt tcgtagtgac cctgcatggt gcccagcggg
     5101 gtttcaatga ttgcaccgct ggtgtactgg tactcttcgc caggcgcgat aagtggctgg
     5161 acgccaacca ctccttcgcc ctggacttcg gtttcacggc cattgccatt ggtgatcagc
     5221 cagtaacgcc ccaacaactg cactggcgct cgccccagat tgcgtatggt tacggtataa
     5281 gcaaaaacgt aacgttcatt atcaggtgaa gattgtgcct caatgtagac gctttgaacc
     5341 tgaatacaca ctcggggcga attgatcatc gttaactctc ctgcaaaggc gcgttctccg
     5401 ccagatagtt cgccatctgg caatattgcg cgacagagat attttccgct cgcatcgccg
     5461 ggtcgatccc cattcccgtt aacacctcga cgcnnnnnnn nnnaatggtt ttacgacgct
     5521 ggttaaaggc ttcggtggtg atgcggctca acacgcgaac atctttaacc gggtgaggca
     5581 tcgttgcatg aggaaccagg cgcacgacgg cggaatccac tttgggtggt ggtgtaaagg
     5641 ctgacggcgg tacttccagc accgggatca cgttgcaata gtattgcgcc atgacgctta
     5701 atcgaccata cgccttgctg ttcggtcctg caaccagacg attcaccact tctttttgca
     5761 acataaagtg catgtcggca atggcatcag tatagctaaa cagatggaac atcaacggcg
     5821 tggagatgtt ataaggcagg ttgccgaaaa cacgcagcgg ctggcccatt ttcgcggcca
     5881 gttcgccaaa gttaaaggtc atcgcatcct gctgataaat cgtcagtttc gggcctaaga
     5941 atggatgcgt ttgcagacgt gccgccagat cgcggtcaag ttcgatgacc gtcagctggt
     6001 ccagacgttc gccgaccggt tcggtcaatg ccgccagacc ggggccgatt tcgaccatcg
     6061 cctggccctt ctgcgggtta atggcagaaa caatgctgtc gatcacgaac tgatcgttga
     6121 gaaagttttg cccgaagcgt ttacgggcta agtggccctg gtggactcga ttattcattg
     6181 ggtgttaaca atcattttga tggcgagatt aagcgccgta ataaaactgc cgacatcggc
     6241 tttaccacgt cccgccagtt caagcgcggt gccgtggtcc actgatgtgc gaataaaggg
     6301 caggcccagc gtaatgttca caccgcgccc gaagccctgg tattttagca cgggaagacc
     6361 ctgatcgtgg tacatcgcca gcacggcgtc ggcgttatcg agatatttcg gctgaaacag
     6421 ggtatcggca ggcagcggcc cgttgagttt catcccctgt tcccgcagct cgttgagtac
     6481 cggaataatg gtgtctatct cttccgtacc catatgaccg ccttcgcccg cgtgcggatt
     6541 cagcccgcag accagaatgc gcggttcggc aataccaaat ttggtgcgca aatcgtgatg
     6601 caaaatagca atcacttcgt gcaaaagggc agggatgata gcatctgcga tatcgcgcag
     6661 cggtaaatgc gtcgttgcca gcgccacgcg aagttcttcg gtcgccagca tcatcaccac
     6721 ttttttcgcc tgcgaacgct cttcgaagaa ctcggtatga ccggtaaacg gaatgcctgc
     6781 gtcgttaatg acgcctttat gcacggggcc tgtgatcagc gcagcaaatt catcgttcag
     6841 acagccatca cacgctcgcg ccagcgtttc caccacatag tgcccatttt caaccgctaa
     6901 cagccccaca gtgacagatt cacgtagcgc gacaggaagt agcgttnnnn nnnnnngagg
     6961 gtgagcggca aaccgagcat cgctgcccgg tcggtaagga gagtggcatc ggcacaaaca
     7021 accagttcga ctggccactc acgctgtgca agctggacaa ttaagtccgg gccaatcccg
     7081 gcgggctcgc cgggagtgat cacaacacgt tgggttttaa ccattagttg ctcaggattt
     7141 taacgtaggc gctggcacgt tgttcctgca tccagcttgc tgcttcttcc gagaacttac
     7201 ggttcatcag catgcggtat gcacgatctt tctgtgccgc gtcggtttta tcgacattac
     7261 gggtatccag cagttcgatt aaatgccagc cgaatgaaga gtgaaccggt gcactcattt
     7321 gacctttgtt caggcgggtc agggcgtcac ggaaggccgg atcgaaaata tctggtgtag
     7381 cccagccgag atcaccgccc tggttagcag agcctggatc ctgagagaac tctttcgctg
     7441 cggcagcaaa agtcgtttta ccactcttga tatcggcagc aatctgttcc agtttcacgc
     7501 gggcctgttc gtcagtcatg atcggcgacg gtttcagcag aatatggcga gcatgaactt
     7561 cggtcnnnnn nnnnnttttg ctttcgccgc gcaggtcgtt aactttcaga atatggaagc
     7621 caacgccgga acgaatcggg ccaacaatgt cgcctttctt cgcggtgctt aatgcctggg
     7681 cgaagatccc cggcaactcc tgaatacggc cccagcccat ctggccgccg ttcagcgcct
     7741 gctggtcggc agaatgagca atcgccagct taccgaaatc agcgccgtta cgcgcctgat
     7801 cgacaatcgc gcgcgcctgg ctttccgctt cgttcacctg atcagaggtc gggttttccg
     7861 gcagtgggat caggatgtgg ctcaggttca gctcagtgct ggcgtcgttt tggttaccca
     7921 cttgctgcgc cagggattcg acttcctgcg gcaggatggt gatgcgacga cgcacctcgt
     7981 tgttacgcac ttcagagata atcatctctt tgcggatctg gttacgatag gtgttgtagt
     8041 tcagaccatc gtaagccaga cggctgcgca tctgatccag cgtcatgttg ttctgtttcg
     8101 cgatgttagc aatcgcctga tccagctgct catcggagat tttcactccc attttctgcc
     8161 ccatctgcag gatgatttga tccatgatca aacgttccat gatttggtgg cgnnnnnnnn
     8221 nncatcaacg tcgctttcca gcacgacgcc gttattgacg acggctgcga ctttatcgac
     8281 tacctggggg gcagcgaaac tggtattcgc gatcatggcg ataccgagaa gcagcgtttt
     8341 ccagttcttc atactttttc catttcaatt aaccgcactg cggattacgt ggtaaatcaa
     8401 caaatcacaa agtgttttga tacggcagaa tgttcgaacg cagcatctct tgcgtaccca
     8461 gaccgtagtt ggagctcagg ccgcgaagtt cgatgttaaa gccgattgcg ttgtcatata
     8521 ccgcatgttg tttatcgtta tcccaaccgt tcagcttccg ctcgtaaccg acgcgaatcg
     8581 catagcagca ggagctgtat tgcacaccta acatagagtc ggcttgcttg ttcgcattgg
     8641 tgtcgtagta gtaggcccca acaatggacc aacgatcggc aattggccag ctggcgacgg
     8701 cacctacctg cgaaatacca ttcttatatt gctcagcagt ggaatagtac ttaggcagcg
     8761 ttgcctgaat atattccggg ctggcgtaac ggtaattcag ctgtaccaga cggtcttcat
     8821 cccgacggta ttcaatgctg gagttactgg tcgctacgtt atccagacgt gtatcgtact
     8881 gaatcccgcc acgcaatccc caacgatcag agatgcgcca gtaagtatcg cctgcccata
     8941 ccagtgagcc cgttttgtcg tcattctccc atgttatgtt gtcatcgcca gtgcgagact
     9001 ccgtgaaata gtagatttga ccaacggaaa tattaaaacg ttcaacggca gcatcatcat
     9061 atatgcgaga tgtgacaccg gtcgtcacct ggttagcgga ggcaatacgg tcaagaccgc
     9121 cgtaagtccg gtcccggaac aggccggagt agtcagattg cagcagcgac gagtcgtagt
     9181 tatagatttt gctctgatcg cgatatggca catacagata ctgcgcgcgt ggttccagtg
     9241 tctgggtata gcccggcgcc agcatttcca tatcacgctc gaagaccatt ctaccgtcta
     9301 ccttgaactg cggcattaca cggttagcgg actctgccag cctggtcgtg tttctggagt
     9361 tataccagtc aagattggtt tgctgataat gggttgccag caacttcgct tcagtattga
     9421 tnnnnnnnnn natgctgccc cagttattag agagcggcag attgatggtc ggttccaggt
     9481 gaacacgagt cgcctctggc atgtcatctc tggtgttaac aaagtgcacc gcctggccgt
     9541 aaattcgcgt atcgaaagga ccgacatcgt tttgatagta gttaacgtct aactgcggtt
     9601 ctgccgagta gctactggtg ttctgctcgc tgaagacctg gaactgcttg gttgaaacgg
     9661 tggcattgaa gttttgcacc gcatagccaa cgctgaattt ttgcgttgca tagccgtcgg
     9721 tactggaacc gtatttgtta tcgaagtcat tgaagtagct aggatcgctg actttggtgt
     9781 agtcgacgtt gaaacgccac acctgatcca tgaccccgga gtgtttccag tagaacaacc
     9841 aacgacgtga actgtcatcg ttcgggtgtt catctttata gactttatct gaaggcagat
     9901 agtccagttc catcaagcca gcgcccgcct gggagaggta gcggaattcg ttctcccaca
     9961 tgatgttgcc acgacgatgc atataatgcg gcgtgatggt ggcatccata tttggcgcga
    10021 tgttccagta atatggcagg tagaactcaa agtagttggt ggtggtgtac ttggcgttcg
    10081 ggatcaagaa accagagcga cgtttgtcac ccaccggcaa ctgcaaatac gggctataaa
    10141 agatcggtac cggacccacc ttaaagcggg cgttccagat ctccgcaact tgttcttcgc
    10201 ggtcatgaat aatttcgcta cctaccacgc tccaggtgtc agaacccggc agacaggagg
    10261 taaagctacc gttatccaga atggtatagc ggttttcgcc acgttgtttc atcaggtccg
    10321 ctttaccgcg accctggcga cccaccatct ggtaatcacc ctcccagacg ttggtatctt
    10381 tggtgttcag attcgcccag cctttcggcc ctttgaggat cacctggtta tcgtcgtaat
    10441 ggacattacc gagcgcatca acggtacgta ccggctccgg ttgtcctggt gcctcttttt
    10501 gatggagctg cacttcgtcg gcctgcagac ggctgttacc ctgcatgata tccacgctgc
    10561 cagtaaacac ggcgtcatcc gggtagtccc ctttcgcgtg gtcagcattg atagtcacgg
    10621 gtaagtcatt ggtatcgccc tgtaccagag gacggtcata gcttggcacg cccaacatgc
    10681 actgtgaggc gaggtcggct gccagtccct gttgactata aagggcggtg gcaatcatgg
    10741 tggccaggag agtggggata cgttttttca tacgttgatt ttattgttcc atcatcggta
    10801 acgttgcgcg tgacaaacgg tcagagacta acgtactcgt catctctacg ctagtgttaa
    10861 tcctgtccga atagcgtctg tggtgttagg cacggcattg aatgacaggt atgataatgc
    10921 aaattatagg cgatgtctca caatagaccg cagccagaaa tgggtaaaag cgcctttata
    10981 ttgtgggaga tagccctgat atccgtgtgt cgatttgggg aatatatgca gtattgggga
    11041 aaaatcattg gcgtggccgt ggccttaata atgggcggcg gcttttgggg cgtagtattg
    11101 ggcctgttaa ttggccatat gtttgataaa gcccgtagcc gtaaaatggc gtggttcgcc
    11161 aaccagcgtg agcgtcaggc gctgtttttt gccaccactt ttgaagtgat ggggcattta
    11221 accaaatcca aaggtcgcgt cacggaagct gatattcata tcgccagcca gttgatggac
    11281 cgaatgaacc ttcatggcgc ttcccgtact gcggcgcaaa atgcgttccg ggtgggaaaa
    11341 tcagacaatt acccgctgcg cgaaaagatg cgccagtttc gcagtgtctg ctttggtcgt
    11401 tttgacttaa ttcgtatgtt tctggagatc cagattcagg cggcgtttgc tgatggttca
    11461 ctgcacccga atgaacggac ggtgctgtat gtcattgctg aagaattagg gatctcccgc
    11521 gctcagtttg accagttttt gcgcatgatg cagggcggtg cacagtttgg tggcggttat
    11581 cagcagcaat ctggcggtgg taactggcag caagcgcagc gtggcccgac gctggaagat
    11641 gcctgtaatg tgctgggcgt gaagccgacg gatgatgcga ccaccatcaa acgtgcctac
    11701 cgtaagctga tgagtgaaca ccatcccgat aagttggtgg cgaaaggttt gccgccagag
    11761 atgatggaga tggcgaagca gaaagcgcag gaaattcagc aggcctatga gctgataaag
    11821 cagcagaaag ggtttaaata agtttcaacg tgtaggcctg ataagacgcg gcccgcgtcg
    11881 catcagacaa tcggcgcaaa attgccggat gcggcgtgaa cgccttctcc ggcctaccga
    11941 acttcgccag cgtgactatt aaaaatccgc tggtgcttta aatgtcatac tattgccata
    12001 cgccggatgg gtaatcgtca gcatctctgc atgtagcaac aaacgcggtg ccattgctct
    12061 cgcttctggt gatgcataaa aacgatcgcc gagaatcgga tgacccagcg ccagcatatg
    12121 cacacgcagc tgatgcgaac gcccggtaat cggttttaac accactcttg ccgtgttatc
    12181 cgccgcatat tccaccactt catactccgt ctgcgcaggt ttacctgttt cgtaacagac
    12241 tttctgtttc gggcggtttg gccagtcgca aatcagcggc agatccacca gaccttccgc
    12301 aggggatgga nnnnnnnnnn atggatgccc ccagacgcgg gccacatact gttttttcgg
    12361 ctcgcgctcg cggaactggc gttttaactc ccgctccgcg gctttggtca gcgccactac
    12421 aatcacgccg ctggtagcca tatccagacg atgcaccgat tctgcctgcg gataatcacg
    12481 ctgaatgcgc gtcatcacgc tgtctttgtg ctcttccaga cgacccggca cagacaacaa
    12541 accgctcggc ttgttgacca ccataatatg gtcatcctga tacaggacaa ccagccaggg
    12601 ttcctgcggc ggattgtagt tttccatccc catcttcggc tccgttactg atgcgtcaca
    12661 acgatcaaac gcagggcatc cagacgccag cctgcctgat ccaggctttc cattacctgc
    12721 tgacggttgc tctcaatggc ggtcagttcg tcgtcacgaa tgttcgggtt aactgcacgc
    12781 agcgcttcca gacgagacag ctcggcagac agtttttcgt cggcttcgtt acgcgctgca
    12841 tcaatcaatg cacgggcaga tttctcgatc tgcgcttccc ccagctgaag gatagcgtga
    12901 acatcctgct gcacggcgtt aaccagcttg ctgccggtgt gacggttaac cgcgttaagc
    12961 tggcggttaa aggtttcaaa ctctacctgc gccgccaggt tgttgccgtt tttatccagc
    13021 agcatacgta ccggcgtcgg tggcaggaag cggttgagct gcaactgctt cggagcttgg
    13081 gcttcaacca cataaatcag ttccaccaac agcgtaccta ccggcaacgc tttgtttttc
    13141 aacagtgaaa tcgtgctgct accggtatcg ccagaaagga tcagatccag accgttgcgg
    13201 atcagcggat gctcccaggt aataaactgt gcatcttcac gcgccagcgc cacttcacgg
    13261 tcgaaggtga tggtgatgcc atcttccgac aggccaggga agtccggcac cagcatatga
    13321 tcggacggcg tcagcacgat catgttgtcg ccgcgatcgt cctgattgat accgataata
    13381 tcgaacaggt tcatggcgaa ggcgatcagg ttggtatcgt catcctgctc ttcaatgctt
    13441 tctgccagtg cctgggcttt ttcgccaccg ttggagtgga tctccaacag gcggtcacgc
    13501 ccctgttcca gttgtgcttt cagcgcttca tgctgctcgc ggcagttttt gatcagatcg
    13561 tcaaagcctt cggtttgatc cggtgaagcc agatagttaa tcaaatcgtt gtacacgcta
    13621 tcgtagatag tgcgtccggt cgggcaggtg tgctcgaatg catccagacc ttcgtgatac
    13681 cagcgcacca gcaccgactg agccgttttt tccagataag gcacatggat ctgaatatcg
    13741 tgcgcctgac cgatacgatc cagacgacca atacgctgct ccagcagatc cgggttgaat
    13801 ggcaggtcaa acatcaccat atggctggcg aactggaagt tacgtccttc agaaccgatt
    13861 tccgagcaca gcagtacctg tgcgccggtg tcttcttcgg caaaccaggc ggcagcgcgg
    13921 tcacgttcga taatcgacat gccttcgtgg aacactgcgg cgcgaatacc ttcacgttcg
    13981 cgcagnnnnn nnnnncagtt gcagcgcagt ggccgctttg gcgcagatca ccagcacttt
    14041 ctgagagcga tggctggtca ggtagcccat cagccattca acgcgcggat cgaagttcca
    14101 ccaggtggcg ttatcgcctt caaattcctg ataaatacgc tccgggtaga gcatatcgcg
    14161 agcgcgatct tccgcacttt tacgtgcgcc cataatgccg gagactttaa tagcggtctg
    14221 atactgcgtc ggcagtggca gcttaatagt atgcagctcg cgtttcggga agcctttcac
    14281 accgttacgg gtgttacgga acagcacgcg gctggtaccg tggcgatcca tcagcatcga
    14341 aaccagttcc tgacgggcgc tctgggcatc ttcgctgtcg ctgtttgctg cttgcaacag
    14401 cggctcgata tcctgctcgc cgatnnnnnn nnnnatggca acggcgtccg caaccggacg
    14461 ataatttttc tgctcttcaa cgaattgcgc aaaatcgtgg aaacggttcg ggtccagcag
    14521 acgcagacgg gcgaagtggc tttccatccc cagctgttcc ggggttgcgg tcagcagcag
    14581 aacgcccggc acgtgctctg ccagttgttc aatggcctga tattcacggc tcggcgcatc
    14641 ttcgctccac accaggtgat gcgcttcatc gaccaccagt agatcccatt cggcttcaca
    14701 gagatgttcc agacgctgtt tgctacgacg ggcaaaatcc agcgagcaaa tcaccagctg
    14761 ttcggtatcg aacgggttgt aagcatcgtg ctgagcttcg gcataacgct catcatcaaa
    14821 cagagcaaag cgcaggttga aacggcgcag catttctacc agccactgat gctgtaaggt
    14881 ttccgggacg ataatcagca cacgttcagc agcgccagag agcagttgct gatgcaggat
    14941 catcccggct tcaatggttt tccctaaacc cacttcgtca gccaacagga cgcgcggcgc
    15001 gtggcggcga ccaacatcat gagcgatgtt gagctggtgc gggatcaggc tggtacgctg
    15061 accgcgcagg ccgctgtacg gcatacggaa ctgttcgctg gaatacttac gcgcgcgata
    15121 acgcagcgca aagcggtcca tacggtcaat ctgcccggca aacagacggt cctgcggttt
    15181 gctgaacacc agtttgctat caaggaaaac ttcacgcagg gccacgccgg actcttcagt
    15241 atcaaggcga gtaccgatat aggtcagcaa gccattttct tcttttactt cttcgacttg
    15301 catctgccag ccgtcatggc tggtaatggt atcaccaggg ttgaacatca cgcgggtcac
    15361 gggggaatca ctgcgtgcgt acagacggtt ttcaccagta gatgggaaaa gtaaagtgac
    15421 agttcgcgca tccaccgcga caacggttcc aagtcccaat tcgctttctg tatcgctgat
    15481 ccagcgttga ccaagtgtaa aaggcatatg tgttcggctc tatatcttta attgcaggca
    15541 ataaccaccc gctaccgtgc ttatgaggta gtggtgttat tcaggtccag gaatggaaag
    15601 ggcgctatgg tactggatgg caaagcattc gtcacgcatc aaaatggtat ctggcgaact
    15661 cttttttttg ctcaaaatag cccaagttgc ccggtcataa gtgtagcaaa attatcctca
    15721 ataaaaggga gtattccctc cgctacgggt tgtagctggc gggtcagata gtgttcgtaa
    15781 tccagtggtg aacgttggta gtccagcggc tccgggccgt tggtggtcca tacgtactta
    15841 atggtgccgc ggttctgata ttgcaagggg cgaccgcgct tatggttttc ttcatcggca
    15901 aggcgagcgg cgcgtacatg aggcggaaca ttacgctgat attcgctcag cggacggcga
    15961 aggcgtttac ggtaaaccag cggcgtgtcc agttcgcccg ccatcagctt gtcgatggtt
    16021 tcgcgcacgt attcctgata tggctcgttg cggaagatgc gcaggtaaag ctcctgctga
    16081 aactgctggg ccagcggcgt ccagtcggtg cgcacggttt ccagcccttt aaacaccatc
    16141 cgctgtttgt cgccctcctg aatcagtccg gcgtagcgct ttttgctgcc ggtatcggcc
    16201 ccgcgaatgg ttggcatcag aaaacggcag aaatgggttt catactccag ttccagtgcg
    16261 ctggtcagcc gttgtttttg cagcgtttcc gcccaccagg cgttaacgtg ctgcaccagt
    16321 gcacgaccga ttttcgctgc ttcttcttcc gaatgtgcac ctttcagcca gacaaacgtt
    16381 gagtcggtat cgccgtagat aacatcatag ccctgcgctt caatcaacgt tttggtttgc
    16441 cgcatgatct gatgaccacg catggtgatc gacgatgcca ggcgtggatc gaagaaacgg
    16501 caggcggtgg tgccgagcac aacataaaag gccttcataa tgaacttnnn nnnnnnngcg
    16561 cctgcgaaag cggcttatta ccctgacgtt tggcttcatc gcgcccgtgc cagatgttag
    16621 tcacaatctc cggcaggcaa tgtttctccc gtgagaacca ggcatcgaga aaaccttcgg
    16681 tactgtgctc tggatcaggc tgcgccatgc cttccaccag cccgacggga tcaatcagaa
    16741 aggtgcggat gatcgacggg tacaggcttt tgtagtccag caccagcacc gaatcataaa
    16801 gcccaggtcg cgaatccatc acgtagccgc cagggctggc gtgcggcggc acttcgccga
    16861 gattaggcgc gacataacca gcgcgatgca tacggggaaa atagagatga ccaaacgctg
    16921 ccaccgaacc gccgtgtcga tctaccggca ggccgttcac cgtcgcccgt tcgagcaaaa
    16981 acggcataat ttcagttttn nnnnnnnnnt gcgtcaccag ctcgcaatct ttcaggttat
    17041 aagttgccag cgcaggctta tcttcggcga aacggcggtc aatttcgtcc attcgatccc
    17101 acgggttatc gatagatttt ccttcgccta ataactcctg agcgacggtt tccagcgaga
    17161 atgaagagaa attccagaac gcggatttca gcgcctcgat accgtcgata atcagtcgcc
    17221 ctttggcctg agcaaaaaag acgccgttnn nnnnnnnngt gctcgcgcca ctccagctcg
    17281 ctgttatcac gccccagacg cagcggaata cggtaacgct cggcatgttt ttgcagcatt
    17341 cgcagatcga actgcaccac gttccaaccg atgatcacat caggatcatg agtcgcaaac
    17401 caggcgttaa gtttttccag tagcagcggg cggctggcga cgtattccag ttcaaaatcg
    17461 agcgcggagg cgtcgccatt ctctggcccc agcatataaa cgatacgctg cccgcaacct
    17521 tccaggccga tgcagtacag ctcaccgtgg cgggtggttt caatatcaat agaaacccac
    17581 ttgagcggcg gacgatagtc gggatgctgt ttcagacggg cattaacgat agcgccgttg
    17641 cgcatatcac cctcgaccca cactggcgag gtgataaacc gctccatcag atagcgttct
    17701 ggcgggcgca catcggcctc gtagacggta acgccagctt cacgcaggcg cttttcgtaa
    17761 ttcatcaact ggcgatgggc gcgacagtaa aggccataca ccggctggcg atgaaaatcc
    17821 tttaacgcca gcggcgtcag gcgaaagcct tgctcaccct gcaaaatatg ctgggcgcgg
    17881 ggaacctgat cggcgggaat aaacgccacg gactcttgcg gcgcaagcgt aacctgcaac
    17941 ggcccgttgt ccgtcgctaa ccaaaaggaa acttcggttc cttgcggggt gtcccgccag
    18001 tgtcgggtta agataaaacc tgcctgcgcc acgctgaaaa tccaataaaa aaccaggctt
    18061 gattatagcc tggtttcgtt tgattggctg tggttttata cagtcattac tgcccgtaat
    18121 atgccttcgc gccatgctta cgcagatagt gtttatccag cagcgtttgc tgcatatccg
    18181 gtaactgcgg cgctaactga cggcagaata tccccatata agcgacttct tccagcacga
    18241 tggcgttatg caccgcatct tcggcgtttt ttccccatgc aaatgggccg tgagaatgga
    18301 ccagcacgcc gggcatttgc gctgcatcga taccctgttt ttcgaaggtt tctacgatga
    18361 cgttaccggt ttcccactca tattcaccgt tgatttctgc gtcggtcatt ttgcgggtgc
    18421 agggaatggg accgtagaaa tagtcggcgt gggtggtacc ggttgccgga attgactggc
    18481 ccgcctgcgc ccagatggtg gcgtggcgcg agtgtgtgtg cacaatgccg ccaatagacg
    18541 ggaatgcctg atagagcagt cggtgagttg gcgtgtcgga ggagggcttt ttcgtacctt
    18601 caaccacttc accggtttcg atgctgacca cgaccatatc gtcagcggtc atgatgctgt
    18661 agtcgacgcc ggaaggtttg atcaccagga cgccgcgccc gcgatcaacg gcgctgacat
    18721 tgccccaggt gagcgtgacc aggttgtgtt ttggcagcgc caggttggct tctaatacct
    18781 ggcgtttgag atcttctaac atgttgactc cttcgtgccg gatgcgcttt gcttatccgg
    18841 cctacaaaat cgcagcgtgt aggcctgata agacgcgcca gcgccgcatc aggcggtgaa
    18901 tgccggatgc gctttgctta tccggcctac aaatccggaa cgcgtaggcc tgataagacg
    18961 cgtcagcgtc gcatcaggcg ttacatacct gatgcggcta cttagcgacg aaacccgtaa
    19021 tacacttcgt tccagcgcag cgcgtcttta aacgctggca ggcgggtatc gttatcaatc
    19081 actgtgattt caatgtcgtg catctcggcg aactggcgca tatcgttgag gttcagcgca
    19141 tggctgaaga cggtatggtg cgcgccaccg gcaaggatcc acgcttcgga agcggttggc
    19201 agatccggtt gcgctttcca cagcgcattc gccaccggca gtttcggcag ggagtgcggt
    19261 gttttcaccg tgtcgatgca gttaaccagc agacggtaac gatcgccgag atcaatcagg
    19321 ctggcgacaa tggctggacc ggtttgagtg ttgaagatca ggcgggcagg atcgtcttta
    19381 ccgccaatac cgtgatgctg aacgt
//
LOCUS       DAWDRC010000011        18881 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1574_length_18881_cov_2.634814, whole genome shotgun sequence.
ACCESSION   DAWDRC010000011 DAWDRC010000000
VERSION     DAWDRC010000011.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18881)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18881)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1574_length_18881_cov_2.634814"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5969..5978
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    15459..15468
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4553 a         4658 c         4967 g         4683 t
ORIGIN      
        1 tcagacacgc gcctggttgt cgaccgcgag catacccagc gcgcgggaga atttttgcgt
       61 taaatagccg gtttcgttac tcgacgcgga agcacacagc atcccggtgg agagccagcg
      121 gttaacagtc acgccttcgg cgttttgcgc aatgtagtta gcatcgcggt cttctttcat
      181 cagtttggcg atgcgatcaa acgcctcttc ccaactgatt tgttgccatt tatcagaacc
      241 tggcgcacgg tattccggaa acttcagacg gctttcggag tggatgaaat ccaccaggcc
      301 agcgcctttc ggacaaagtg ctccgcggtt gaccgggtga tccgggtcac cttcgatatg
      361 gaagatagat gctttggcgt tttttgctcc gtcaccgagg ctgtacatca acagcccaca
      421 gcctacggaa caataggtgc aggtattacg ggtttcgcgg gtgcgcagca gtttatactg
      481 ccgggtttcc gcgagtgcta cgctgggtgc aaaacccagt gccgctgccg tggtgcctgc
      541 cataccgcca gcgcagatct taaagaactg ccttctgctg acctgcatgg attgctcctt
      601 gtttcgacat tgtcacgtcc catttacatt cgcttgctgc gtgtgcaggg agtgggagtt
      661 atttttcttt gcggaagggg ccgcaaaggt ccagaattgg ctcaatttcc ctccatccag
      721 gaagggtttg taacagaata ccataatgtt ggtgtgtgtg ttcttatctg gttaatagaa
      781 agtgaaaaaa acacagcaaa aagaaatcga gaatgtgaca aatatcacag gtgttcgtca
      841 aattgagtta tggcggcgtg atgatttaca acacccacgg ttagatgagg tggcggaaga
      901 agttcccgtt gcgctggtct acaacggcat ttcgcacgtg gtgatgatgg cgtcgcccaa
      961 agaccttgag tactttgcgc tcggtttttc gctttccgaa ggaattatcg aaagcccgcg
     1021 cgatatcttc ggcatggatg tcgttccttc ctgtaatggc ctggaagtac aaattgagct
     1081 ttccagccgc cgctttatgg ggttgaagga gcgccgccgg gcgctggcgg gacgtacggg
     1141 ctgtggcgta tgcggtgtag aacagcttaa cgatatcggt aaaccggtgc agccgctgcc
     1201 gttcacccag atgtttgatc tcaacaaact ggatgatgca ttacgtcatc tcaatgattt
     1261 ccagccagtg gggcaactga ctggttgtac tcacgccgct gcatggatgt tgccatctgg
     1321 cgaactggtc ggcgggcatg aagacgtggg tcgccatgtg gcgctggaca aactgttagg
     1381 ccgccggtca caagaagggg aaagctggca acaaggtgcg gtgctggttt ccagccgtgc
     1441 cagttatgaa atggtgcaaa agtcggcgat gtgcggcgta gagattttgt ttgcggtgtc
     1501 tgccgcgacc acacttgctg tagaagtggc tgagcgctgt aatctgacgc tggtgggctt
     1561 ttgtaaaccg ggtcgggcaa cggtttatac ccatccgcag cggttgagca attaattaaa
     1621 ataagtcctg tgatatatat taaattgcag gacttattca tttcgtgaat tttgttattt
     1681 tttcataaac aaaacgcgtg tagtggcgtt aacgcctgcg ctgcaaccta ttgatggcgt
     1741 ggcggtgagt tatattgatg ctgccgtagc gttgggtaat accattaacg aaatggataa
     1801 atattacacc caggaaaatt ataaagacga tgcgtttgcg aaagggaaaa cattccacta
     1861 ctactctctg gcagtcatga tttcagcgaa acaaattaat aacctgatat cgcaagataa
     1921 gtttgatgca gaagcggcaa tgaagaaagt gtctgaactg gaaacgctgg tggcgcaagc
     1981 caaagaagcg gataaaagcg gcatgaattt ctcgtttatt aattcggcag gccagtatca
     2041 actcgaggct aaaaaatacg ttcgccgcat cagagataaa gtcctgcact ctgactggga
     2101 taaagagcaa cttcaggacg caaactcaag ctggatggcc gaagactcat ttccacgagc
     2161 gttatgcgat tacaacgaaa tggtcgatga aatattacag ttaattgtaa tcgcggggcg
     2221 ggtttgtgat gagcacggat atgtcacgaa gtcgtaacaa tataaagcag agtgaaggat
     2281 aaattttgtg aataaagtat cattatttaa tgattttgtg aatgttaaaa tgtctcagat
     2341 aatttgttta agcgttattt atatttgtgg cttatgagtc agcgtgaatc atatctaata
     2401 ttttttacga tatttagtta aaaacggcac tattgttata ttcctcacgt tccaggaaat
     2461 cagcggattt actcatatta tgcggcggga tactatccat cggcctgctg aagttggatt
     2521 aaaaattcga aagtgtacac cttatatttc tctatatacg tattcctttt ggaaagcgta
     2581 gggtctcctt tatttcaggg aacgtaaata atgaaaaaat cgacattatc tttagccatc
     2641 ggtttattat tggcatgtag taccggtatg gcaaaaacac agcatttaac gctggaacaa
     2701 cgcatggcat tgctggaaga acgcctggaa gcggcagaaa tgcgggcagc aaaagcagag
     2761 gggcaggtta aacagcttca gacacaacaa gccgccgaga tccgcgaaat taaaaccgca
     2821 cagggcaaca cgccggtaaa cggtcaatca acgacggagt cagagaagaa aaacgccacc
     2881 ccgcctaatc tcctgctttc agggtatggc gatttaaaaa tctacggtga cgtagaattt
     2941 aatatggatg cggaaagtaa tcatggcctg ctggcaatga ccaacgctga tgtgaatagc
     3001 gatcccacta atgaacagtg gaatctcaat ggtcgtattc tgttaggttt tgatggtatg
     3061 cgaaaactgg ataatggcta tttcgctggg ttctccgcac aaccgctggg ggatatgcac
     3121 ggttcagtaa atatcgatga tgcggttttc ttctttggca aagaaaacga ctggaaggtc
     3181 aaagtaggcc gttttgaagc ctacgatatg ttcccgctga atcaggatac ctttgttgaa
     3241 cattccggta atactgcgaa cgatctttat gacgatggca gcggttatat ctatatgatg
     3301 aaagagggcc gcggacgttc taacgctggc ggtaatttcc tcgtcagcaa acaactcgat
     3361 aactggtatt ttgaattaaa cacgttactg gaagacggaa catctttata taacgacggt
     3421 aattatcatg gacgcgatat ggaacagcag aaaaatgttg cttatctgcg tccggtaatt
     3481 gcctggtcgc cgacggaaga attcaccgtt tccgcagcga tggaagcgaa cgtggtaaat
     3541 aatgcttatg gttataccga tagcaagggt aattttgtcg atcagtccga tcgtaccggt
     3601 tatggcatga gcatgacctg gaatggcctg aaaaccgatc cggaaaatgg catcgtggtt
     3661 aatcttaata ccgcctattt agatgctaat aatgaaaaag atttcaccgc tgggattaac
     3721 gcgctgtgga aacgtttcga gctgggttat atctacgcac ataataagat tgatgaattt
     3781 agtggcgtgg tttgtgataa cgattgctgg attgatgatg aaggaacata caacattcac
     3841 accattcatg cgtcttatca gttcgctaat gtgatggata tggagaactt taatatttac
     3901 ctcggcacgt attactccat tctggatagc gacggcgata agatacacgg cgacgatagt
     3961 gatgaccgtt acggcgcacg cgttcgcttt aaatacttct tctgatgaaa gggcggtgct
     4021 ggatattttc cggcaccgca actcattaag gcaggtaaaa cacttcttgc agttcgctac
     4081 tgactgggct gttatccgca ttagcgggca taacgtcggt catatatttc caccaacgtt
     4141 ggcaaacatc tgtactggca accgcattcc accgctcttc agattcaatc tctaccgtgg
     4201 caaatagcag attatgcgct ttgtcgagat agatggcgta gttatgcgca ccgtgagatt
     4261 tcagcacggc ttccagttcc ggccagatgg gattatgccg acgctgatac tcttcgtggg
     4321 cgtcggggtt tacctgcatg acaaaggctt tgcggatcat gtttgctcct tacagcgcca
     4381 gcgcactggc gtcggcccgc tgccgaacac accgtggaag ggccacaaca ccagcgaatg
     4441 tttttgcatc tcttgtgcag ttgcctggcc gatttcgtcc gtgccgggta ccatccacgg
     4501 cagaatgcca acgccatccg ggaataccac cagacactcg gtgctgcctt cccacagttg
     4561 gcgagtgaag accgcggtgt cgttttccag cacgtaggtg agagcgatca ggttagtggc
     4621 gtggcagtgc atgatcaccc gatctttgcc gttggtggct ttaatgcgct cgaagtggga
     4681 aaggaagtga gctggaagtt cggaagtggg gacggcttcg ttggttaacc cccaaagaat
     4741 gtggtagccc gcgccgtcgc tgtcgacttt tacgatgcct aagttagccg caggatcaag
     4801 ctggacgtta cggaagaatt tgccggagcc ggtgacaata aacggtgtat ttgcaagtaa
     4861 aggcatgggc tggctgagcg ggatatagcg cggttgttgg tgaaaattgt cgtgatatgg
     4921 tgcgatatcg gcgtcatcca ggcgcagcgt caggttgccg ccgttgcgct cgtcccagcc
     4981 tttcagccag gcatcggtgg tggctttgat cattccctgg acaaaccagg actgggttat
     5041 gttttgcatg ttctgtgttc ctgttaattg gacgtccgtg cctgttgccg gatgtacgat
     5101 ctgtaggccg gataaggcac tcgtgccgca tccggcagtg tttacccgcg gcgactcaaa
     5161 atttctttct cataagcccg cacgttctcc agccattcgc tacccgctgg cgtatcgtga
     5221 cgctgacaat acatttccca taccgcctgc cacggcaacg atttctgctc ttccagtagc
     5281 gccagacgcg cagtgtaatc gcccgccgct tccagcttgc gcagctcagc ggtaggttcc
     5341 agtaacgcac gtagcagggc ttttttcata ttgcgtgtac caatgaccca cgcggcaatg
     5401 cggttgatag aggcatcgaa gaagtcgagg ccgatatgca cccggtcaaa cagatcgtga
     5461 cgaacaatct cactggcaat cgcctgggtt tcatcatcca gcagcactac gtgatcgctg
     5521 tcccagcgaa ccggacggct gacgtgcagc agcaactgcg gcacatacag catggcggcg
     5581 gaaatcttgt cggaaatcac ttcagtcggg tggaaatgcc cggcgtccag gcacagcgcc
     5641 gtctggcggc tggtggcata ccccaggtaa aactcattgg agccaaccgt gtagctctcc
     5701 gcgccaatgc caaacaattt gctctcaacg gcgtcgatat ggtgcgcagg gtttagcttc
     5761 tcgctgatca cttcatccag tgctgccagc aaacgctggc gtggggcgag acggtcaacg
     5821 gtaatatctt tcatgccatc cgggatccag atgttcatca ccgacggtgt gccgagttgc
     5881 tcaccaaaat aggctgaaac acggcggctg gctttacagt ggtcaatcca gaactggcga
     5941 atgcggtcgt cggcatggga aagcgtaann nnnnnnnngc gtaaagccat cggcgcttag
     6001 cggatgcgaa aaacaggagg ggttaaaatc cagaccgagc tgattggctt tcgcccattc
     6061 aacccagttt ttgaagtgct ctggtttgat ctggtcgcgc gagactggcg tgtctgattc
     6121 cagatagatg gcatgtaaat taagccgttt cggccctgga atcagccgca tagcctgttc
     6181 cagatcggca cgtagctcac tggcattacg cgctttgccc ggataattac cagtagcctg
     6241 aataccaccg gtcagcaaac cttccgggtt ttcaaaaccg gaaacatcat cgccctgcca
     6301 gcagtgcatt gaaacgggta aacgatcaag ttggcgcagt gcctcctcga catcaatccc
     6361 tactgccgcg aaacgctgtt tcgccagctc ccaggcctgt tccagttgag tggtcatgcg
     6421 caaagctcct ttgtctgtcg tgtagagtga atctgcgcca catagtgggc aatttcactg
     6481 tcaggattag gggtaaaggt ggtcagattc gcggtagtgc tgacgacctg acggaaatca
     6541 tccacattgt tgagttcatc cagcgtcatt aactggatgc cgatattgcc gagcgtcgag
     6601 gcttcaacag gccctgcgat cacccgaata ccgcaggcat cggcacacag ctggttgagc
     6661 agcgtgttct ggcagccgcc gccgacaata tgcagttgcg agaaatcttc accacgcagc
     6721 tgcgccagct catgcaacac atcggcatac agcagcgcca gactgtcgaa aatacagcgc
     6781 gccagttcag catcactttc cgggatcggt tgcgccgttt cccgacacgc agcctgaatt
     6841 tcgctgcaca tctcgtcagg attaataaag cgatcgtcat tgggattgat gatgaagcgg
     6901 caggccggaa gtgcctgtgt tgcggcgata agcgccggaa gatcgttgat ttgccgctcc
     6961 tgaagcactc gctgaagcag ccataagccc ataatatttt tcagcacccg atagcgacct
     7021 tccgccccgc cttcgttggt gatattggct gccagcgccg tgtcattggt aaatggcgtc
     7081 tggctttcga agcccatcaa tgaccaggtg ccagaagaga gataagcggc gcgtgaaccg
     7141 tttaacggcg aggcgataac cgcgctggcg gtatcatggc tggtaacggc gaccaccgga
     7201 atctcattac cctgcgggca aatccagtga cctatgacat tacccggatg cgtcgggcga
     7261 ccaaaccagg ctttgttggc accgctccac gccagtagcg actcgtccca gtcgtcgcta
     7321 ttgatattga ccagttgcgt ggtcgtggcg ttggtatatt cccagttcat cttgccggtc
     7381 aggcgataac taaagtaatc cggtatcagc aaagcgtgag caatgtgtgg aataagttct
     7441 ggttgctgct ctgtcagtgc acgtagctga tagatcgtat tgaagggcag aaactggatg
     7501 ccgctacgtt gataaatatc gcgtttgccg agttgctgtt gtgcctgcgc cattaggcca
     7561 ttggtgcggc tatcgcggta agcaacgggc agacccacgc gctgaccgtg ctggtcgagc
     7621 agcacaaagt ccacgcccca ggtatcaatc ccaatgctat cgatacgaat cccttcctcg
     7681 cacaccttgt ttagcccaag gcgaatggca ctttccaggc tatccacatc ccaggtgaca
     7741 taaccgttct ggctatgcag cccattttta aaacgatgga tttcgcgcag cgtcaggctg
     7801 cggcattcac gctcgtaacg cgccagcatc acgcgcccac tggatgcgcc gagatcgacg
     7861 gcgacacaat tgcgaaaggt cataatgtga tcctgctgaa tttcattacg accagtctaa
     7921 aaagcgcctg aattcgcgac cttctcgtta ctgacaggaa aatgggccat tggcaaccag
     7981 ggaaagatga acgtgatgat gttcacaatt tgctgaattg tggtgatgtg atgcttaccg
     8041 catttcctga aaattcacgc tgtatcttga aaaatcgacg ttttttacgt ggttttccgt
     8101 cgaaaattta aggtaagaac ctgacctcgt gattactatt tcgccgtgtt gacgacatca
     8161 ggaggccagt atgaccgtat tacatagtgt ggattttttt ccgtctggta acgcgtccgt
     8221 ggcgatagaa ccccggctcc cgcaggcgga ttttcctgaa catcatcatg attttcatga
     8281 aattgtgatt gtcgaacatg gcacgggtat tcatgtgttt aatgggcagc cctataccat
     8341 caccggtggg acggtctgtt tcgtacgcga tcatgatcgg catctgtatg aacataccga
     8401 taatctgtgt ctgaccaatg tgctgtatcg ctcgccggat cgatttcagt ttctcgccgg
     8461 gctgaatcag ttgctgccac aagagcagga tgggcagtat ccgtctcact ggcgcgttaa
     8521 ccacagcgtg ttgcagcagg tgcgacagct ggttgcacag atggaacagc aggaagagga
     8581 aaatgattta ccctcgaccg ccagtcgcga gatcttattt atgcaattac tgctcttgct
     8641 gcgtaaaagc agtttgcagg agaacctgga aaacagcgcg tcacgtctca acttgcttct
     8701 ggcctggctg gaggaccatt ttgccgatga ggtgaattgg gatgccgtgg cggatcaatt
     8761 ttctctttcg ctgcgtacgc tacatcggca gcttaagcag aaaacgggac tgacgcctca
     8821 gcgttacctg aaccgcctgc gactgatgaa agcccgacat ctgctacgca acagcgaggc
     8881 cagcgttact gacatcgcct atcgctgcgg atttagcgac agtaaccact tttcgacgct
     8941 ttttcgccga gagtttaact ggtcaccgcg tgatattcgt cagggacggg atggctttct
     9001 gcaataacgc gaatcttctc aacgtatttg tacgccatat tgcgaataat caacttcgtt
     9061 ctctggccga ggtagccacg gtggcgcatc agttaaaact tctcaaagat gatttttttg
     9121 ccagcgacca gcaggcagtc gctgtggctg accgttatcc gcaagatgtc tttgctgaac
     9181 atacacatga tttttgtgag ctggtaattg tctggcgcgg taatggtctg catgtactca
     9241 acgatcgccc ttatcgcatt acccgtggcg atctctttta cattcatgct gacgataaac
     9301 actcctacgc ttccgttaac gatctggttt tgcagaatat tatttattgc ccggagcggc
     9361 tgaagctgaa tcttgactgg cagggggcga ttccgggatt tagtgccagc gcagggcaac
     9421 cacactggcg cttaggtagc gtggggatgg cgcaggcgcg gcaggttatc ggtcagcttg
     9481 agcatgaaag tagtcagcat gtgtcgtttg ctaacgaaat ggctgagttg ctgttcgggc
     9541 agttggtgat gttgctgaat cgccatcgtt acaccagtga ttcgttgccg ccaacatcca
     9601 gcgaaacgtt gctggataag ctgattaccc ggctggcggc tagcctgaaa agtccctttg
     9661 cgctggataa attttgtgat gaggcatcgt gcagtgagcg cgttttgcgt cagcaatttc
     9721 gtcagcagac tggaatgacc atcaatcaat atctgcgaca ggtcagagtg tgtcatgcgc
     9781 aatatctcct ccagcatagc cgcctgttaa tcagtgatat ttcgaccgaa tgtggctttg
     9841 aagatagtaa ctatttttcg gtggtgttta cccgggaaac cgggatgacg cccagccagt
     9901 ggcgtcatct caattcgcag aaagattaat tcgccatgcc gatgccgacg atgttagcgg
     9961 cgacaataat caccacacaa ccgaggctca acaccgttac cggacggcgt cctgcattgt
    10021 tccactcttt cagcaccagc ccgacgatac cgccgcacaa tacatagaag ctcatatgca
    10081 gcatccagct gatgtagtca tactgcgccg gaatgcgggc gtggccccag gcatagaaaa
    10141 agaattgcag ataccacatc aacccgccca gtgctgagag taacacattg tgaatgatca
    10201 gcggttttgc cagcgagaag tcggctttta gcgacaaatc tttcactttt gccagacgaa
    10261 tgaagcagaa gccgaggttg atgattgcgc cgccgcccat gatgacaaca tagcttggca
    10321 gagcgacata caggggatcg acgcccagcg cggcagcggc ttcatgcatc ggttttgcgg
    10381 cgttcatcgc aaaggacatc ccggcagaga aaatgccgca catcaccgcc agcaccagcc
    10441 cttttttcag attgaactct tcggctttaa tgcccatctt gcgctctttc aactgcccgg
    10501 cgcgagttac aatccctacg ccaatcagcg ccaccagaac gccgagcaac gtcatgcgtc
    10561 cgccttcggt attaatcagc acatcgaaat tgccgttgat aattggcgtc atcagcgtac
    10621 cgacaatcaa cgtaatgcca atggcgatgc caattcccat cgacatgccg agataacgca
    10681 tggtcaggcc gtagttgata ttaccgatcc cccacatagc gccgaacaga aaaacaggca
    10741 gcagcgtaga gagactaaac gagctgtaat acgcccagaa attcggtagt aacagggcgc
    10801 tgatggccca cggcaggata atccacgaaa caatcccacc gactgaccac atggtttccc
    10861 atgaccattt ttttactttt ttgaacggag cgtaaaaaca ggctgcactg gccgcgccga
    10921 tcaaatgcca aaatatcccc atcgtaatcg cgttactcat cttcttatcc tcatcatttt
    10981 ttcgtcgcgt cacatctccg acgagatgag tgtaaaaatt gagctgtcga ttaacctttc
    11041 gcctgttgcc gccgctgccg atttactggc aatcacggca ttaagtgggt gatttgcttc
    11101 acatcttggg cattttcctg caaaaccata cccttatgaa aagtacggca ttgataatca
    11161 ttttcaatat catttaatta actataatga accaactgct tacgcggcat taacaatcgg
    11221 ccgcccgaca acactggaga tgaatatgag ttataccctg ccatccctgc cgtatgctta
    11281 cgatgccctg gaaccgcact tcgataagca gaccatggaa atccaccaca ccaaacacca
    11341 tcagacttac gtcaacaacg ccaacgcggc gctggaaaac ctgccagaat ttgccaacct
    11401 gccggttgaa gagctgatca ccaaactgga ccagctgcca gcagacaaga aaaccgtact
    11461 gcgcaataac gctggtggtc acgctaacca cagtctgttc tggaaaggtc tgaaaaaagg
    11521 caccaccctg cagggtgacc tgaaagcggc tatcgaacgt gacttcggct ccgttgataa
    11581 cttcaaagca gaatttgaga aagcggcagc ttcccgcttt ggttccggct gggcatggct
    11641 ggtgcaaaaa ggcgataaac tggcggtggt ttctactgct aaccaggact ctccgctgat
    11701 gggcgaagcc atttctggtg cttccggctt cccgattgtg ggcctggatg tgtgggaaca
    11761 tgcttactac ctgaaattcc agaaccgccg tccggactac attaaagagt tctggaacgt
    11821 ggtgaactgg gacgaagcag cggcacgttt cgcggcgaaa aaataatcat ttgatgcctg
    11881 ctgcaatgag gcgtttacgc cgcattgcag cttaaaaaat gaaccatcgc caacggcggt
    11941 ggtttttttg tgatcaattt caaaataaaa acaatgatcc gataaaaata aaacagcgtt
    12001 tcaattgatg tggttttgat cacttttatt gatcaaatga atgtctatct tcgtttccat
    12061 caacactgat gctccgttga ggaatgacgc atcagccctt aaaaatatgc cgacaggtga
    12121 tggaaattca gataaaacgc tcgattgaga aaatcccggg ggggatgatg ctcgtcccgc
    12181 tattccttgg cgcactgtgc cacaccttct cgccgggggc ggggaaatat tttggatcat
    12241 tcaccaacgg gatgattacc ggtacggtgc ccattctggc ggtgtggttt ttttgcatgg
    12301 gggcgtcaat aaaattaagt gcgacgggaa cggtactgcg taaatccggt acgctggtgg
    12361 taactaaaat tgccgtcgcg tgggtggttg cggcaattgc ctcgcgcatt attccggaac
    12421 atggtgttga agttggattt tttgccggac tttcaacgtt ggcgctggtg gcggcgatgg
    12481 atatgaccaa cggcggactt tacgcttcca tcatgcagca gtacggcaca aaagaagaag
    12541 ctggggcatt tgtgttgatg tcgttggagt ccgggccgct catgacgatg attattctgg
    12601 gcactgccgg gattgcctcg tttgaaccgc atgttttcgt cggcgcagta ttgccgttcc
    12661 tggtgggctt tgccctgggg aaccttgagt tacgagaatt ttttagcaaa gcggtgcaga
    12721 cgctgattcc attctttgcc ttcgcactgg gcaataccat tgatttgact gtaattgccc
    12781 agacaggttt gctggggatc ctgttgggtg tggcagtaat tatcgtgacc ggtattccgt
    12841 tgattatcgc cgataaattg attggcggtg gcgatggcac tgccggaatt gccgcttcca
    12901 gttccccagg ggccccggta gcgacacctg tgctgattgc agaaatggtg cctgcgttta
    12961 aacctatggc tccggcagca acttcgctgg tcgcgacggc ggtcattgtg acttcgattc
    13021 tggtgccaat ttttacctct atctggtcac gtaaagtcaa agccagagcg gcgaaaatcg
    13081 aaattttagg tacggtgaaa taaaccaact tccttaatcc ccacagccgg atgaactaac
    13141 gttcgtccgg ttttcaattg cgtttctgcc tggatactta ccgcgaaaac agcacgcctg
    13201 acataaaata aatatggtct aatggggaaa tttctcgtgg agagggaaca gatgcgatat
    13261 ccggttgatg tatacacagg caagattcag gcttatcccg aaggcaaacc cagcgcaatt
    13321 gccaaaatcc aggttgatgg tgagttgatg ctgacggacc tggggctgga aggtgacgaa
    13381 caggcggaga aaaagatcca cggcgggcca gacagagcgc tatgtcatta tcctcgtgag
    13441 cattatctct actgggcgcg ggaatttccg gaacaggcgg agctgtttgt tgcgcctgcg
    13501 tttggcgaaa atctctcaac cgacggtctg acggaaagta atgtttatat tggtgatatt
    13561 ttccgctggg gagaggcatt aattcaggtc agccagccac gctcaccttg ctataaactc
    13621 aattaccatt ttgatatcag cgatatagcg cagttgatgc aaaacacggg taaagtgggg
    13681 tggttgtata gcgtaattgc accagggttg gtatctgctg atgcgccact ggagttggtt
    13741 tcccgtgtca gcgatgtgac cgtgcaggag gctgccgcca tcgcatggca tatgccgttt
    13801 gatgacgatc agtatcatcg tttactctcc gccgcagggt tatcgaaaag ctggacgcga
    13861 acgatgcaaa agcgccgact gagcggcaag atcgaagatt tttcccgccg tttgtgggga
    13921 aaataacccc ggagtgtagg cctgataaga cgctatcagc gtcgcatcag gcatcctgct
    13981 caaatgccgg atgcggcgta aacgccttat cctgcctaca aatgcggagt ttaactccgc
    14041 ttatacagcg gcaaccaaat caccagccgt aaaccgccca gcgggctgtc ttctgccttc
    14101 acccagccac gatgctgctg aatggcggtt tctacaatcg ccagccccaa acctgtaccg
    14161 ccagattcac gatcgcgtgc ttcatcggtc cgatagaatg gacggaaaat ctgttcgcga
    14221 tcttccgggc taacgccagg accatcgtcg tccaccgtaa tggtgatacc gtctttatct
    14281 accgcaaagc ccacttcaat cttcgtatgg gaataacgca gagcattacg aacaatgttt
    14341 tccagcgcac tttccagggc gttcgggttg ccgtacagcg gccacggccc aggtgggaag
    14401 ttaaccgtca acgacttgcc catttgctcg gcttcgaacg ccgcgttatc cagcacttca
    14461 ctccacaact ggttggcttt gatggtctcg ctaaccagcg catttttctg ctgattacgt
    14521 gacatcacca acagatcgtt gatcatgctg tccagacgtt gcgcttcggt ttcaatacgc
    14581 tccagttcct tgctttcacc gctacgacgg cgcagtaacg ccgtacccag ttgcagacgc
    14641 gtcagcgggg tgcgcagttc gtgagagata tcagaaagta gacgctgctg agaggtcatc
    14701 atgcgctcca gcgcggtgac catctggtta aaactggcac cggcggcaag gaattcctgt
    14761 ggccccgctt ccagttccgg gtgctggcgt aagtttccct gggcaacttc atcggcagcg
    14821 tttttcagct tacgcgccgg ttttgccaga ctccaggcca accacaacag cagcggcgta
    14881 ctgaccagca tggtgacaat cagcagtaat agcgggcggt caaacagtaa gtttatgaaa
    14941 tcggattgag aactgctggc cggacgaatc agataaagtt ggtaattatc ttcgccatca
    15001 cgcacggaga acggaccgac cagttccacg cggccatact ttttcttctg aggatgatcg
    15061 gcatttcgct gcgttcagcg ccgatcacgc ggccttcggt ggtcaccaat aacaaacgct
    15121 gtcctggcgg tgcccactta tcaatcgccc ggaacagacg ccgccaccac attaaatcgt
    15181 tgggcggatc gttcgccagc tccgcttcga catgctgctc aatcatcaga ccctgacgct
    15241 gttcgctatc cagaagctcg gtcatctggc gtgaatcgag cttgggtaac atcaaaacca
    15301 acatcaacac cagcgccagc gtcagccaga agatggcgaa gatgcgcgcg gttaagctgc
    15361 ctatcatgaa gcagaaacca tcagatagcc gcgaccacgc agggttttaa accacgggtg
    15421 accatcttta cgatccggca gtttacgacg caggttggnn nnnnnnnngt gcatatcgat
    15481 agcacggtcg aaaggcgtca ggcgcttgcc cagcacttcc tggcttaaat gctcacggga
    15541 aaccacctga cccagatgct gtgccagcaa atagagcagg gtaaactccg taccggttaa
    15601 ctccagcgtt tgcccgtcga aggtggcttc ctggcgacca ggattgagac ttaaggcatc
    15661 aacttccagt gtcggtgaac cgttgtcgtt gttttgctgt tgttcgctcc agtgcgaacg
    15721 gcgcaggatc gcgcgaatac gtgccaccag ctcacgatca ttaaacggtt ttgggagata
    15781 gtcatctgcg cccagctcaa gcccgagaac gcgatcaagt tcactgccgc gcgccgtcaa
    15841 cataatgaca ggcgtctggt gtgtctggcg aagtgctttt aatgtgtcga taccattttt
    15901 cttcggcatc attacatcaa gcaaaagtaa atcaatgctg tcgtccagaa gatcaagcgc
    15961 ctgttcccca tcgtgggcaa caatcacgtt gaagccttcc atctcgagca gctcctttaa
    16021 tagggaagtc agctctcggt catcatcaac taacaggatt ttattcattg tttaaatacc
    16081 tccgaggcag aaattacgtc atcagacgtc gctaatccat gactttacgt tgttttacac
    16141 cccctgacgc atgtttgcag cctgaatcgt aaactctcta tcgttgaatc gcgacagaaa
    16201 gattttggga gcaaatgatg cgcatagtta ccgctgccgt catggcctca acgctggcag
    16261 tcagttcatt aagccacgct gctgaagtcg gttcaggcga taactggcat ccgggtgaag
    16321 aacttacgca gcgcagtacg cagagccata tgttcgacgg cataagttta accgaacatc
    16381 agcgtcagca gatgcgagat cttatgcaac aggcccggca cgaacagcct cctgttaatg
    16441 ttagcgaact ggagacaatg catcgccttg tcaccgcaga aaattttgat gaaaacgctg
    16501 tgcgcgcaca ggcagaaaaa atggcgaatg agcaaattgc tcgtcaggtt gagatggcaa
    16561 aagtccgcaa ccaaatgtat cgcctgttaa cgccggagca gcaagcggtt ttaaacgaga
    16621 aacatcaaca acgaatggag cagttgcgcg acgtgacgca atggcaaaaa agttcatcgt
    16681 tgaagctatt gagtagtagc aactcacgtt cccagtagta aaccctgttt tccttgccat
    16741 agacaccatc cctgtcttcc cccacatgat gtgggggttt tttttatcct caatttgcct
    16801 gctgcttaat gcattgcaga tgatttgctt ccgttatact accgtcagtt gatagcggga
    16861 gtatttatga atcaatctta tggacggctg gtcagtcggg cggcgattgc tgcgacggcg
    16921 atggcttcgc tgctattgct gattaaaatt tttgcatggt ggtataccgg gtcggtgagt
    16981 attctcgccg cgctggtgga ttcgctggtg gatatcggcg cgtcgttgac gaatttactg
    17041 gtggtgcgat attccctgca acctgccgac gataatcact cgtttggtca cggtaaagct
    17101 gagtccctcg cggcgctggc gcaaagtatg tttatctccg gttcggcact attcctgttt
    17161 ttgacgggta ttcaacatct ggtatctcca acaccgatga cagatccagg cgtcggggtt
    17221 atcgtgacaa ttgtggcgct aatttgtacg attatccttg tctcgtttca gcgttgggtg
    17281 gtgcgccgga cgcaaagcca ggcggtgcgg gctgatatgc tacattacca gtctgatgtt
    17341 atgatgaacg gcgcaattct gctggcgctg gggttgtcct ggtacggctg gcatcgcgcc
    17401 gatgctctgt ttgcattggg gatcggcatc tatattttat atagcgcgtt acgcatggga
    17461 tatgaggcgg tacagtcatt actggatcgc gcattgcctg atgaggaacg gcaagaaatt
    17521 attgatatcg tgacttcctg gccgggtgtt agcggcgctc acgatcttcg cacgcggcag
    17581 tcagggccga cccgctttat tcagattcat ttggaaatgg aagactctct gcctttggtt
    17641 caggcacata tggtggcgga tcaggtagag caggctattt tacggcgttt tccaggatcg
    17701 gatgtgatta tccatcagga cccctgttcc gtcgtaccca gggagggtaa acggtctatg
    17761 ctttcataat cagtataaaa gagagccaga cccgcatttt gtgtataaaa taccgccatt
    17821 tggcctgacc tgaatcaatt cagcaggaag tgattgttat actatttgca cattcgttgg
    17881 atcacttcga tgtgcaagaa gacttccggc aacagatttc attttgcatt ccaaagttca
    17941 gaggtagtca tgattaagaa aatcggtgtg ttgacaagcg gcggtgatgc gccaggcatg
    18001 aacgccgcaa ttcgcggggt tgttcgttct gcgctgacag aaggtctgga agtaatgggc
    18061 atttatgacg gctatctggg tctgtatgaa gaccgtatgg tacagctaga ccgttacagc
    18121 gtttctgaca tgatcaaccg tggtggtacg ttcctcggtt ctgcgcgttt cccggaattc
    18181 cgcgacgaga acatccgcgc cgtggctatc gaaaacctga aaaaacgtgg gatcgacgcg
    18241 ctggtggtta tcggcggtga cggttcctac atgggtgcaa tgcgtctgac cgaaatgggc
    18301 ttcccgtgca tcggcctgcc gggcactatc gacaacgaca tcaaaggcac tgactacact
    18361 atcggtttct tcactgcgct gagcaccgtt gtagaagcga tcgaccgtct gcgtgacacc
    18421 tcttcttctc accagcgtat ttccgtggtg gaagtgatgg gccgttattg tggcgatctg
    18481 acgttggctg cggccattgc cggtggctgt gaattcgttg tggttccgga agttgaattc
    18541 agccgtgaag acctggtaaa cgaaatcaaa gcgggtatcg cgaaaggtaa aaaacacgcg
    18601 atcgtggcga ttaccgaaca tatgtgtgat gttgacgaac tggcgcattt catcgagaaa
    18661 gaaaccggtc gtgaaacccg cgcaactgtg ctgggccaca tccagcgcgg tggttctccg
    18721 gtgccttacg accgtattct ggcttcccgt atgggcgctt acgctatcga tctgctgctg
    18781 gcaggttacg gtggtcgttg cgtaggtatc cagaacgaac agctggttca ccacgacatc
    18841 atcgacgcta tcgaaaacat gaagcgtccg ttcaaaggcg a
//
LOCUS       DAWDRC010000012        18725 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1593_length_18725_cov_2.777504, whole genome shotgun sequence.
ACCESSION   DAWDRC010000012 DAWDRC010000000
VERSION     DAWDRC010000012.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18725)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18725)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1593_length_18725_cov_2.777504"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    830..839
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2092..2101
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12835..12844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13656..13665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4887 a         4437 c         4542 g         4819 t
ORIGIN      
        1 gaaccctcac gttcaacctt actctcattt acacgttacg cttttttaaa gcgggcaaag
       61 agatgttcca ctacaacgaa aaagaccgga acgaagtaaa ttgccagcac tgttgcggca
      121 aacatcccgc ccattacgcc ggtacctacc gcgttttgcg cgccagagcc ggcaccatga
      181 ctgataacca gcggcagcac gccgagaata aaggccagag aagtcatcag gattgggcgt
      241 aaacgcatac gcgctgcttc gatgattgcc tctaccggcg ttttcccttc tttctgcatc
      301 atctcaacgg caaattcgac aatcaggatg gcgttcttgg cggaaagccc gatggtggtc
      361 agcaaaccaa cctggaagta aacgtcatta cttaagccgc gcagatcggt ggccagtaat
      421 gcgccaacga cgcctaacgg aacaaccaac atcaccgaga acggaattga ccagctttca
      481 taaagtgcgg cgagggcgag gaacaccacg accagtgaaa tggcatatag cgcgggagcc
      541 tggtttgagg ataacgcttc ctgatacgac agtccggtcc atgagtagcc gacgcctgcc
      601 ggaagttttg cgaccaggtc tgccataaat ttcatggcgt caccggtact tttcccggca
      661 gccgcttcac ctaaaatctc cattgacggg atgccgttgt agcgttccag tcgcggtgaa
      721 ccataggtcc attcggtaga cgaataggca gaaagcggtg ccatcgtgcc agaggcgttg
      781 cgtacatacc attggttgat gttatccggc aacatacgga acggcgtgcn nnnnnnnnna
      841 tcagaaagcg ccacacccat agcctcggct ttcgcggcgt tgacgttcac tttgaacatc
      901 ggcgtatctt ccaggccgtt cgggcgtacc ccgatgacct gattcggtga ttgcgctgcc
      961 agtgataaca gctcgtttcg cgcctgggtc agcttttcgt gccccagatt gccgttgtcc
     1021 agcagttcca tatcaaaacc tgacgcggta cccagttcag ccaccgcggg taagttgaac
     1081 gggaagacga cggctttatt gatactgctt agcgcaatca ttgcccgctg aataatcgcg
     1141 gtaaccgagt tttcctcgcc gacacgttca gaccacggct tgaggctgat aaacgccaga
     1201 ccgttgtttt gcccctgacc actgaagcca aagccaccaa cggtaaacac cgactgaaca
     1261 ttatttttct cttttgtcag ataataatcc gtcacctgtt gtagcacttt cgtggtgtta
     1321 accatggttg caccggaagg taactgcgcg gtggtcataa ataccccctg atcctcttct
     1381 ggtaagaaag aggtcggcgt acgcaggaac agcaccgcca tcccggcgca aatcagcagg
     1441 tagaccacca tgtagcgacc ggtacaacgc aacagcgagc gggtgctgtc ggtatagtgc
     1501 tgagttgatt tttcaaacag cgtgttgaag cgtgcgaaca gggcgttagg tttgtgaccg
     1561 ccctccggcg cggctttcag aatggtggcg cacagggcag gggtcaggct cattgctaca
     1621 aatactgaaa gcagcatgga ggagatcagc gtgatggaga actggcggta gatctccccg
     1681 gtcgcaccgc tcataaaggc catcggcata aacactgcgg aaagaacaac ggcaatcccg
     1741 accagcgcac gttggatctg ccccatcgat ttatgcgtcg cttccttcgg cggtagctta
     1801 tcttccgcaa tgacacgctc gacgttctcc accaccacga tggcgtcatc caccagtaac
     1861 cctatcgcca gcaccatccc gaacatagtt aacgtgttga tggtgaaacc gaccgccgac
     1921 aagatcgcaa acgtcccgag aataaccacc ggtacggcaa tcgtcgggat gattgtggcg
     1981 cggaaattct gcaaaaacag atacatgacc aggaagacga ggatgatagc ctcaaccagc
     2041 gttttgaaaa cttcctgaat agaaattttg ataaacggtg tggtgtcgta annnnnnnnn
     2101 ngtgtcgtaa ggataaaccg tcttcaggct tgcagggaaa taagccgata aacggttcag
     2161 ctcctctttc accgctcgcg aggtatccag ggcgtttgct ccggtagcca gtttgatggc
     2221 gatcccagca gcaggtttgc cgttatagcg cgccacggtg gaataatctt ccgccccaag
     2281 ttcgacgcgg gcgacatcac gcagcagtac ttgcgaacca tcttgctgaa ctttcaacag
     2341 gattttgcca aattcttccg gcgtttgcag acgcgtctgc acaatgatcg aggcgttcag
     2401 ttgctggtct gccgcctgtg gcatgccacc cagttgacca ccggaaatct ggttgttttg
     2461 caccttaatc tgggaaataa catcggaagg taccaggtta tatttattga gtttttgcgg
     2521 gtccagccag atacgcatgg cgtactcgga accaaagagc tgtacgctac caacgcccgc
     2581 ggtacggctt agcgggtctt tgatattaga cgctacatag tccgcgatat cgtactggtt
     2641 gaggctgccg ttatcagaaa taaatgccgc taccatcagg atattactgc tcgacttatc
     2701 gacgctaatc ccctgctgct gcaccgcttc aggtaatgaa ggcatagcga gttgcagttt
     2761 attttgcacc tgaacctgtg cgatatcagg tgatgtcccc gtctcgaagg tcagagtgat
     2821 ggaggcatta cctgccgcat cgctggttga agacatgtac atcaggccat caagcccatt
     2881 catattttgc tcaatcacct gagtgaccga gtcttctacc gtttgcgcat cggcacccgg
     2941 ataggtagcg ctgatggtaa tggtcggtgg cgcaatctgc ggatactgcg caaccggtaa
     3001 gttcatgatc gccagaccgc ctgcaagcat cataataatg gcaagtaccc aggcaaaaac
     3061 cgggcgatca ataaaatagt tagccatgaa agtcccctta agcctgcaac gttattgttt
     3121 cgattcggtg ctggcgtttt cctggctgga ggaaattgca cgtgctttga tacccggacg
     3181 aatgcgttgc aagccggaaa cgatcacccg atcgccagcc tgcaagccag aggtgacaac
     3241 ccactggtcg ccgatggctt tgctggcttc gatttcgcgt agctgcacga catcgtcttt
     3301 atccagaatg agtgccgttg ctttaccctg ggcgttgtgg gtgacgcctt cctgcggcac
     3361 cagtaataca ttctggcggc taccttcatc cactaatgcc gtgacataca tgccaggcag
     3421 caagtcacca tttgggttgg ggaaaatcgc ccgtaacgtc acggagcccg tggtttcatc
     3481 caccgtcggg tcggagaatt tcagcgtgcc ggtctggccg taacgtttac cattttccag
     3541 attgagctgt actggcgtac tgccctgaac ctgtttgatt tgcccactgg cgacctcttc
     3601 tttcatgcgc aagaaatcct gcaccgactg cgtgagatcg acataaatcg ggtccagacg
     3661 ttgtacggta accagcgaat ctgcctgatt agcggtaacg agtgcgccga cggtcaccga
     3721 cgatttcccg ctgacgcccg taatcggcga ggtgacattc gcgtattgca gattgatcgt
     3781 cgcctgttca acagccgctt tggcgacggt gacattggct tctgcttcat tcaactgggt
     3841 gcgtgcggtg tcgtaatcct gacggctaac atagttggtc ttcagcaacg atgcctggcg
     3901 gttaaaggtg atgcgggcat tgctggcggt agagagcgct ttcgccagcg aacctttggc
     3961 ggagttcagc tctgcctgta aaggcgcagg atcaatctga tacagcgaat cgccctggtt
     4021 cactttatcg ccttcgataa agttgcgttt aatgataata ccgcccacct ggggacgtat
     4081 ctcggcaact tcataaggaa cggttctacc gggcaattcg ctcaacacat tgaccgaacc
     4141 gggggagagt gtgacgacac cgacctcagg cgtcatggcg gcggcgtttt ccgccgattt
     4201 gtcatcgcag gcggtgagca tcgcgccgca gaataacaac ggtattaaca gttttcttct
     4261 tctgttcatt ttagtccctg aaaattcttg agaaataacg gttctttaaa ttgcgcgcga
     4321 ggagagatat gagggattta cagaccagcg gcatagtggg gttgtcagcc agtaagatgg
     4381 gatgcaagca cagtgatcga catggtgagg tcaacgacaa aaaaaatgtt gccgttctgc
     4441 caacagtttc tgccagcatt cgggcaacga agggttctac tggtggatac acataccagg
     4501 ggaataaagt cgttttcgtc tctccttcaa taatcaccgg gggtattgaa atcagcatct
     4561 gcatccctcg tcatgccagc catcaatttc agctgcttat gtccttacta aaaataagat
     4621 gtgataccca gggtgacgat gtcgctcata cgtcttaatt tcagcttgta cataatgttg
     4681 aatttctgaa tctttaatgt ttgttgcgaa atgttgagcg ttgacgtgat attgctttca
     4741 tttttgtttt tcagaatcag ttgcataatt ttgaactgat tatgtgaaaa acataattgt
     4801 gaattcttat ggggcaagtg tttaccataa gtaatctcaa gaagattttt gatactttct
     4861 ttacggctga caaaataaat aggcgcttta gcttttagtt tactgatgtg ataaggtgcc
     4921 aaaacaataa ttctctcggc atctaatttc tccaggaatt taatcgcttc ttcatcaagg
     4981 atatggttgt gataggtgtc aatgatcacc ttgaagggtt tattgcctac ttttttgatc
     5041 tctgacaggg aattgatttt tatcatttcg tgattatctt tcaactgcca aaagccctgt
     5101 aaaagaaaag aatctttcgt catgagaaaa atcataactt gctccttagc cgttatcgtt
     5161 tcgaacatgt catccttttc attatttaca tccttgtccg aatcgttgtt caatatagta
     5221 aacgcctata agaactttgc ccttcgtgaa ctaatacgtc tattaacact aatcaatact
     5281 tcagatttat ccatcacaca ctccatatat tggtcctaag taagattcct ggttgttatc
     5341 agtttgtaac tagtaaacaa aaggttaact tttgcggatt actgcccaag aataagttat
     5401 caataaccag tttgtgatct ctgaagaata ttactaaagt taaaatgtaa tccgatttaa
     5461 atatcgagtc tccttgtttc gacttaggct ggcaattgga ttgccagctt tcttttaacg
     5521 cattgaaatt acctgattaa ataactcatt catgtcttca ggtttttata tagcgtctgg
     5581 agacacggat atttatgcaa tgtaatcaac gccagttgca aaaaaatgaa cccggattac
     5641 ttatttaacc gcccatagca tcaacgctta cggaactctt tgttcacttt cccacagcac
     5701 ttttaaaaca ttaaacctga ccacggaaaa tatcagccat gataatgttt gaaatggcta
     5761 atttgccatg gaacgaaaaa aatcagatta aattcagtag gttgaaataa tcaacagcag
     5821 gcaattgttt caatgatagt gcgtaattga actgaaacac tgtatagcta agagagtatt
     5881 aactggatca tagtcactta atgtgactat gatccaggtg gaaataggga taattattgc
     5941 tttttaacga acaaactggc gaagctgaac agactggcgg cgctaaatat cagttcaatt
     6001 cccaccagtg tggaaaccag cgttaccgac accatcggcg tagcaccaag gaatatccag
     6061 gcaatgacga tatccagcac accaataacg agctgtagcc aactgccttt cattgaacgc
     6121 tgacgatacc aactcatcag gcgaataacc cctgccacac agaacaaacc ggcaataaat
     6181 gccgcaatgg caaaaatgcc cagctctggt gcgcggatga agaaatagcc gatcaataaa
     6241 taggcgactg cgacgaggaa accggataat accggccaga aattatgact gcggttgctg
     6301 aatagcccga caataagcgc aatgcccgag cagattaata atgcacccac tactgtgctt
     6361 aaaatgtcgc cagagacgaa cggaaaactg atacacagca acccgacgat aaacagcagc
     6421 acggcaataa actggattgc tctgcgatgt tttttaagca tctccagatc aaacttcaaa
     6481 attgttgcct tatctatata taacatagaa ccaccctata aaattaagaa gaaaatcccc
     6541 tgctatcaat ctatgccaaa aacgcgtgta agaatgcagt cgatttaata aaaatttcct
     6601 aattacagta tctgatgcat ctgtaactca ttgtattgaa ataaaaatat ctgattttga
     6661 tattttccat caacatgaca tatacagaaa accaggttat aacctcagtg tcgaaattga
     6721 ttcgtgacgg ctctttcact ttatagttga ggatattacg atgaaaaaag tattaggcgt
     6781 tattcttggt ggtctgcttc ttctgccagt agtgagcaat gcagcggatg cacaaaaagc
     6841 agctgataac aaaaaaccgg tcaactcctg gacctgtgaa gatttcctgg ctgtggacga
     6901 atccttccag ccaactgcag tcggttttgc tgaagcactg aacaacaaag ataaaccaga
     6961 agatgcggtt ttagatgttc agggtattgc aaccgtaacc ccagctatcg ttcaggcttg
     7021 tactcaggat aaaaaagcca gctttaaaga taaagttaaa ggcgaatggg acaaaattaa
     7081 gaaagatatg taattccggg aatgcgttac atcgtatttc cttgcatatt gaacaggccg
     7141 gaatatcttc tttaaaagca gctattcctc ctgtttgtat ataatctcta tattgaatgg
     7201 gttacaaaat gaacatttca tctctccgta aagcgtttat ttttatgggc gctgtagcgg
     7261 ctttgtcact ggtgaacgca caatctgcgc tggcagccaa tgaatccgct aaagatatga
     7321 cctgccagga atttattgat ctgaatccaa aagcaatgac accagttgcg tggtggatgc
     7381 tgcatgaaga aacagtatat aaaggtggcg ataccgttac tttaaatgaa accgatctca
     7441 ctcaaattcc taaagtgatc gaatactgca agaaaaaccc gcagaaaaat ttgtatacct
     7501 tcaaaaatca agcatctaat gacttgccga attaataagg tacaagtaaa aaggagtagc
     7561 aagttgagcc atcttgctgc tcctttttgc atttttatat gacagcagaa tttattatac
     7621 gacttatact tgcggtaatt gcctgtggcg ctattggcat ggaaaggcaa atgcgcggca
     7681 aaggagcagg gttacgcaca catgtattaa ttggcatggg aagcgccctg tttatgattg
     7741 tttcgaaata tggttttgct gacgtgctgt ctttaggtca cgtcggactg gatcccagcc
     7801 gtattgctgc tcaggttgtg acgggcgtcg ggtttatcgg tgcaggtaac attctcgttc
     7861 gtaaccaaaa tattgtaggt ctgacgacgg cagcggatat ctgggtgacc gccgccattg
     7921 gtatggttat tggcagcggt atgtacgaac tgggtattta tggttcagtg atgaccttgc
     7981 tggtgctgga agtcttccat caattaacct tccgcctgat gaacaaaaat taccatttac
     8041 agctgacatt agtgaacggt aatacggtat caatgctcga ctggtttaag cagcaaaaaa
     8101 taaaaacgga tttagtttcg ttgcaggaaa atgaagatca tgaagtggtc gccattgata
     8161 tccagcttca tgccacgacg tcgatagaaa atctgttgcg attattacaa ggtataaccg
     8221 gggtgaaagg cgtttcaatt agctaatctg tatagttatt tattcgcagg agtaccagtg
     8281 cggtactcct gttggtttta atgatcgagg acgatcagtg gtgaatggtt ggcaggatct
     8341 ttaagtacgg taatatccgc gccgtagagc attgttagcg cctgctgtgt caatacgtcc
     8401 tgcggcttac catttgcgat aacctttcct ttttgcatta atacaacacg gtctgcataa
     8461 cgcgcggcaa gatttagatc gtgcaatacg cagcaaacat tgaactgccg ttcgtgaact
     8521 aactggcgta ataaacgaaa caagtgctgc tgatggtgaa tatccagcgc cgaagttggt
     8581 tcatcaagaa ataaccattt cggtgaggga gtaggttccc agagttgcac cagcaggcgc
     8641 gctaattgca cgcgctgttg ttcaccaccg gataattgcc gatagtcgcg gtttgctaac
     8701 gcctgacagt cacataacgc cattatttgt gctgtttcat tatcctgatt accagtacga
     8761 tgcggatgcc gccccatctg gataacttct tgtacgctga atggaaacgc catgtggcta
     8821 ttttgacgca tcacggcccg gtgtttcgcc agttcagtaa tcgaccattc atttaatggc
     8881 ttattaaaca accgacattc gccgctatca ggttgaagat agccggttag ctggcgaagc
     8941 agcgtagatt ttcccgcacc attaggcccg aggatggcaa caatttcccc accgggaaaa
     9001 gtcagcgaga cattatcagt cagacgccgc ccctgaaggc tataaaccag attctgggcg
     9061 ctgatcatgt gatcctccgg ttgcgcagaa tcaaccacat aaaataaggg ccaccaagca
     9121 ggctggttaa taatcccacg ggcatttctg ctggctgtac cagcgtgcgg gcaagcgtgt
     9181 ctgccatcag caataaacag gctccggcga gggcggaaca agggattagc cagcggtgat
     9241 ttgccccggt agtcatgcga atcagatgcg ggatcaccag gccaataaag ccgataatgg
     9301 cgggtaccga tacggcagca ccaacgagca gtgagctcac taacagtaat tgctggcgtt
     9361 tacgcttcac gttcacgccg aggtagtggg cttcttcatc accgagctgc agtaaattca
     9421 gcgttccggc gagacaagtt gttgcgataa tggccggtag gatgaatgaa ctggcaacca
     9481 ataacgtcgg ccattgcgcc tgtccaagat tgcccatcat ccacaatgtg agttgtcgta
     9541 gctgctgctc atcgccgata tagctcagga tgccgatcgc tgcgccgcac agggcattga
     9601 tggcgatacc ggcgagtaat aattgcatca tgctgccatc gcgatggcgc tgcgtgatga
     9661 gaaaaatgat ggtgcagacc actaaacttc cggcaatggc aaacaccatt tgctcataga
     9721 gcaccagcac cactgggaag gagaagggca gcacaatagc aacgccaacc attaatgcag
     9781 aaccgctact gacgccgagt aatccagggt cagccattgg gttgcgaaaa agcccctgca
     9841 taattacccc ggcggttgcc aacgctgcac ctaccagcac cgccagcaaa acccgtggta
     9901 gacgaatatt gagccagata tcgcggtatt cctcatcaaa aagggcatca aaactgatct
     9961 ttaacgctcc ctgactggca ccaaacagca ccagagctag caataatagt gatagcccca
    10021 taaagaccct ggcggaagag agtgaatcct tgagcacgca aaactccgtt acgacattac
    10081 ggcgcggaca tgccgcgccg ggggatcacg ccggtttcag gtccggttca atcagactgt
    10141 agacctgctg gttaagtgct ggcgcatttg ccagttcatg gatatgtgcg gcgacatcag
    10201 cacggttaat aaaaccatga cactcctgat tttgtatccg ttgtgctttg cccgttgccg
    10261 caccatcaag cagaccgcca gggcggagaa tggcataatc aagctggctg gtttgcaacc
    10321 agctttccgc cagcgttttt tcccgtaccg cctgaccaaa cgcagccttt gcccgctctg
    10381 ataaaaacgg ccagctatcg ccgcatccca gtgacgtcac cagaatcatg cgtgtgatgc
    10441 ctgctttctc ggcctcgtca atcactgttc tgtgcgccag ataatcctga gctccgccca
    10501 ttgttgagat gataagcgca tccgggccag ctgcgcggca agcagccgcg actacgctgg
    10561 catcacaggc gtcgcctgta aaaacctgta cgccttgttg cgccagtttc gtggcggcat
    10621 cggcatgacg aatgacagca acgaccggac gttgttccgc cagcgccagt tcaagcgttc
    10681 tggcacccac accttttccg cctgcgccaa agagtagcca cggtgtcatc aggcgtgttc
    10741 ctttaacgat gctgccagag catggaaggc actgacttgt tcgctcagga gctgacgatg
    10801 atcatcccta ccgaggaaga ttttcagcat tgcgctgcct tccttattga aaaagagaat
    10861 agaggcggta tccatgccca taaatttacg ttcgatcaag gcaatatgcg tgcagttttc
    10921 tgctttgata tgcccggaca tgccgtgctt tccgcgtagg ttgaaatagc cgtggcgatg
    10981 gaaacccgaa ggcagttcgc cgctaaattc gagtattaca tcggtagtat gtactaacgt
    11041 ggtgacgttg ccccattcgc agacggtatc ccagacagta tcgaatttat cacctgacac
    11101 aacagtcgga gagggcagat ttttcactac ctccagcagc gtggtgttgt attgttcggc
    11161 gatcgcctcc agcgtaccgt ctggctccgt tttcaaaaat tcctgtaacg agacatggct
    11221 cataaatcac tcctgttatg gtttttcaag cgcaattgct ggcgcattaa gtacctgaat
    11281 cagttcatta agagactgca aaatgttgct tgcccagaaa cgaccttcat tggtcaaccg
    11341 cagacacgtt gacgcatcgc ggcttaagcc tttttggtgc cactgggcca gtaacggcgc
    11401 gagtttttcg gcatgtggcg taagttcgtc cagcggtaca cgcgccgttt caatgcccga
    11461 ctgcaaggta tgacgccact gcgcgttacg ttcggcgtta cgcatgatca tcatcagcgg
    11521 ttttttacct gctgcgacgg attcatgcca ggtctgaaga ttgcgttcgt tcatccagga
    11581 gtaaccatta atcgaaccgc cggctccgga accaaaggcc agacaatcgg caccttgttt
    11641 aatcagcagg ttatagagat tgcgttcgcg tgtggtacgg ccccagtggc tgttactgat
    11701 gcagcgccaa ccggcatcgt ccataaaatc acacccttgc aggtaaagat cgcgacgttc
    11761 tgccggagag ggcacggtag tacgcccatt ttccacggct ttgcccagcg gtgtattggg
    11821 caggacattg agcgcataga gatcgacgcc gtcgagaccg atatcgcggg caatagccag
    11881 atcttctccc caggtttgcg catcctgacc cggcagacca aacagcaggt cacagaccac
    11941 cgcggcacgg tcgcgtttga ccaggctttc cataaacgca atagcggttg ggccatccga
    12001 ggtgcgggcc attttcttgc ggattttgct gttaaacgac tgaataccaa ttgagaagcg
    12061 gtttgctcca gcatcaaggc aagcatcgat tcgctcagcg tcaaaattca gtacccggcc
    12121 ttaaatagtg atttcacaat caggggccag tggcagcttt tctcgtaatg tggtgatgat
    12181 tctggccaaa tcgtgtgctg aaagcgcgga aggcgtaccg ccaccgaaat agaccgcgtg
    12241 gatgggggca gactgatgca atacgctgtc tgcttccatt tctatttcac gaatcagggc
    12301 gtcggtataa tgagcacacg catcgtcatt aaaacggttc tgataaaaac cacaaaacgt
    12361 gcagtgtgtc gcacaaaaag gaatgtgtag ataaaccagt cgcttacgtg gtgataccgt
    12421 ttgattgatc atctcttgcc aggtctgtgc cagttgctct ttggcaaccg gaatggcccc
    12481 acgaaacggc atcatggcgc gcctgtcttt aaatggctga tcaccgtcta aagcaaaatg
    12541 tggcgtgaga tccagggtgt tatttgtgtt cattcgtctg atttaaatcc atttattgcg
    12601 gccaaagctg ctggtgtaaa gactcaacga catcagcaat acgcggaccc attcccagaa
    12661 tcaggttttg atcaacggtg ataatgcgct ggtttttcca tgcggcagtg tgggtaattc
    12721 cggcaataga acgtagccga ttaatatcgc catcgaccat ttgtgaggtg acgacaatca
    12781 cttcagggtt agccgcaatc agcgattccg cgctgtagct tttgtactgc tggtnnnnnn
    12841 nnnnacgctg cctttaccgg caacctgtgg cgcacttccg cctgcggaga gaataaacat
    12901 ggctttaacc ggggcttttt tggccgccac gttttgctgt actcgctcca ggcgttggtt
    12961 gatctgtgtc accagcgcct cgccttgttc aggaacctgt aacgtttttg ccagttggcg
    13021 aatgttggcg tacatctgct caagggtggc tggtacacgc ggcagagtga cgacgttgac
    13081 tttttgcgcc cgcagttggt cgagcacaat ttgcggtcct gcatcctgcc aggtaattac
    13141 gctatccggg cgaagtgaca aaatgccttc gctgctgagt tgtttccagt aaccaatatg
    13201 cggcagtttg gcggtttctg gtggatagga tgtcgtttca tcgacaccaa ccacgcgttt
    13261 gccagcgccc atcgcgtaga ttagctccgt cagcgatcct cctgcgacca cgatacgttc
    13321 ggcagccgtt acgctaaaag tccagctaag agccagtaat gccgtaaaca atttttttcg
    13381 ttttaccatg gtcttcgcgg cagaggccac ggctgaaata cataacgtta gtggggaaaa
    13441 aagaaacggc ctggtgatga tccttggcat aatgtgagaa ctcttatcaa attgagaatg
    13501 atcatgatta tcatttgagt tatatatgaa atacaaccgt cgattcaata cgcacggcgt
    13561 tacaagcgta tttatttttg atttttatca agatgcttcg tcagctaatg ttcttgttac
    13621 cgattaaatt tcagcgcggg aaaaagctga gaaccnnnnn nnnnngcggc ttgcagagca
    13681 aagagaagag aaaattatca ctgttaacga caacgttttg tgatgaggtt gtggggggat
    13741 agccataaac acaggatggt tttctggatg ctggatagat ttcataaccc cctgtttatt
    13801 gtgttaatgg cggttaaaaa aagcttgatc ataaaaaatt gatcgataat aattctcatt
    13861 atcacgtttg agtttaacaa aatatgacat catggataac aaaatcatat cttttgtaag
    13921 ggaaaacgca cagcactctt cacttcagac gtggcgtact actttgcctg acaaagaggg
    13981 cgtgcttttg ctgaattatc agaaatattc ggcaatttta cgggatatat acgctaatag
    14041 cttcccgtgg tgatatctaa tcatttgaat tgtaaataaa tttatttttc gccagccacc
    14101 ccggtattaa cccaggcagc cagcattttt tattttcctc atggagaatc gctatgtcac
    14161 gtccgcaatt tacttcgttg cgtttgagtt tgttggcttt ggctgtttct gccaccttgc
    14221 caacgtttgc ttttgctact gaaaccatga ccgttacggc aacggggaat gcacgtagtt
    14281 ccttcgaagc gcctatgatg gtcagcgtta tcgacacttc cgctcctgaa aatcaaactg
    14341 ctacttcagc cactgatttg ctgcgtcatg ttcctggaat tactcttgat ggtaccggac
    14401 gaaccaacgg tcaggatgta aatatgcgtg gctatgatca tcgcggcgtg ctggttcttg
    14461 tcgatggtgt tcgccaggga acggataccg gacacctgaa tggcactttt ctcgatccgg
    14521 cgctgatcaa gcgtgttgag attgttcgcg gaccttcagc attactgtat ggcagtggcg
    14581 cgctgggtgg agtgatctcc tacgatacgg tcgatgcaaa agatttattg caggaaggac
    14641 aaagcagtgg ttttcgtgtc tttggtactg gcggcacggg ggaccatagc ctgggattag
    14701 gcgcgagcgc gtttgggcga actgaaaatc tggatggtat tgtggcctgg tccagtcgcg
    14761 atcggggtga tttacgccag agcaatggtg aaaccgcgcc gaatgacgag tccattaata
    14821 acatgctggc gaaagggacc tggcaaattg attcagccca gtctctgagc ggtttagtgc
    14881 gttactacaa caacgacgcg cgtgaaccaa aaaatccgca gaccgttgaa gcttctgata
    14941 gcagcaaccc gatggtcgat cgttcaacaa ttcaacgcga tgcgcagctt tcttataaac
    15001 tcgccccgca gggtaacgac tggttaaatg cagatgcaaa agtttactgg tcggaagtcc
    15061 gtattaatgc gcaaaacacg gggagttccg gcgagtatcg tgaacagata acaaaaggag
    15121 caaggctgga gaaccgttcc actctatttg ccgacagttt cgcttctcac ttactgacat
    15181 atggcggtga gtattatcgt caggaacaac atccgggtgg cgcgacgacg ggcttcccgc
    15241 aagcaaaaat cgattttagc tctggctggc tacaagatga gatcacctta cgcgatctgc
    15301 cgattaccct gcttggcgga acccgctatg acagttatcg cggtagcagc gacggctaca
    15361 aagatgttga tgccgacaaa tggtcatctc gtgcggggat gactatcaac ccgaccaact
    15421 ggctgatgtt atttggctca tatgctcagg cattccgcgc cccgacgatg ggcgaaatgt
    15481 ataacgattc taaacacttc tcgattggtc gcttctatac caactattgg gtgccaaacc
    15541 cgaacttacg tccggaaact aacgaaactc aggagtacgg ttttgggctg cgttttgatg
    15601 acctgatgtt gtccaatgat gctctggaat ttaaagccag ctactttgat accaaagcga
    15661 aagattatat ctccacgacc gtcgatttcg cggcggcgac aactatgtcg tataacgtcc
    15721 cgaacgccaa aatctggggc tgggatgtga tgacgaaata taccactgat ctgtttagcc
    15781 ttgatgtggc ctataaccgt acccgcggca aagacaccga taccggggaa tatatctcca
    15841 gcattaaccc ggataccgtt accagtaccc tgaatattcc gatcgctcac agtggcttct
    15901 ctgttggttg ggtcggtacg tttgccgatc gctcaacaca tatcagcagc agctacagca
    15961 aacaacctgg ctatggtgtg aatgatttct acgtcagtta tcaagggcag caggcgctca
    16021 aaggcatgac cactactctg gtattgggca acgccttcga taaagagtac tggtcgccgc
    16081 aaggcatccc acaggatggt cgtaacggaa aaattttcgt gagttatcaa tggtaatcat
    16141 ctgccccgat atttcggggc atttatctgg aaggaagaga gacaatgaac cactacacac
    16201 gctggcttga gttaaaagaa caaaatcccg gaaagtacgc gcgtgacatc gcaggtttaa
    16261 tgaatatcag cgaagcagaa ctggcatttg cacgcgtcac gcacgacgcg tggcggatgc
    16321 gcggcgatat tcgtgaaatt ctggcggcgc tggaaagcgt tggtgaaacc aaatgtattt
    16381 gtcgtaatga atatgcagtc catgagcaag ttggctcgtt cacaaaccag catttgaacg
    16441 gacatgccgg attgatcctc aatccgcgcg cgctggattt acgtctgttt ctcaatcaat
    16501 gggccagtgt tttccacatc aaagaaaaca cggctcgtgg cgaacgccag agtattcagt
    16561 tctttgatca tcagggcgat gcattactaa aagtttatgc caccgacaat accgatatgg
    16621 cggcatggag tgagcttctg gcacggttta tcaccgatga gaatacgccg cttgagttaa
    16681 aagccgttga tgcgccagtt gttcaaacgc gagccgatgc cagtgtggtc gagcaagagt
    16741 ggcgggcgat gaccgacgtt catcagtttt ttacgttgct caagcgccac aacctgacgc
    16801 gccaacaggc gttcaatctg gtggcagacg atttggcctg caaagtatcc aacagtgcgt
    16861 tggcgcaaat tcttgaatct gcacagcagg atggcaatga aatcatggtg tttgttggca
    16921 accgtggctg cgtacagatt ttcaccggtg tggtagaaaa agtggtgcca atgaaaggtt
    16981 ggctgaatat tttcaacccg acgtttaccc ttcatctatt agaagagagc attgctgaag
    17041 cctgggttac ccgtaaaccg accagcgatg gtcacgtaac cagtctggaa ttgtttgccc
    17101 atgatggtac gcagatagcg caactttatg gtcaacgtac agaaggtgaa caggagcaag
    17161 cgcaatggcg taagcaaatt gcttcgttga taccggaagg cgttactgca taacgtataa
    17221 ttaacgggag gtctggctgt tgctgggcct ctttatattg ttcaatcgtt cacaccagat
    17281 aattaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg atcatcatga
    17341 tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc agaatatctt
    17401 ccgttgtcat ttgattcgca atgcagctca gaacctgatg ctgcttgcgc gagagggaca
    17461 catccgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaag tcgatgatgg
    17521 cgatagttcg cggaacaaag gtcaccggta ctttccccag gcagcgttta atattaaagg
    17581 gggagaaaat aattacatgg acaggtttta atcgttcaat ttgagtgaga aaattagaat
    17641 gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc aacattacgt
    17701 cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg tttttcatcg
    17761 cggtgaaaaa catcgtatcc ctggtaatta taagaaacat aaccgactcc tggtacggac
    17821 attaacttta taataagttt gccaaaggta attaacagga cagggcttat aataatagtt
    17881 agcttactgt gtattaattt gatgtaatgc atgatgcaga tactgagaaa gcatctgcat
    17941 ctttcggtgg tgttatttga ccagctcagg tgttacctga ctcaccgcat tggtgtagta
    18001 aggagccccc cagcccggtt ccggccagaa tgcaccatag ccgtaatccc acaagttata
    18061 ggtggtatta accacttctc tcaaatgcca cacctggatg ccctgcatat tcacttccag
    18121 gaagttatac gggactttat tgataaagcc aggttgttca ccctgaatgg taccgaggat
    18181 ggtaacaaag tgattacgat agttcactgg atcaaggaag ccgctttgtc tggcgagcag
    18241 tcggccctga tagttggctt caatatcagg cttcgcatag ctatccaacg gtaatacagc
    18301 gatttctaac aacgtatccg ttttaccatt gataacgttg ataaccttcc cgccaaagcg
    18361 cgcttgttga ccaacatata accccggctg gttatgaaca gcaacaaaac ttttttgaat
    18421 atcaggttgg ttattgcctt tgatattttg cggaattgaa ctacatgcgg caagcaaaaa
    18481 tgaaaggctg aggatgagtg caccttttgt catgttcatg ttactatcct tatcaacaaa
    18541 tattaaaacc ataatagata tttctttata gttttcatct gattctgagg cgatatgcct
    18601 gatagtaacg taaacctgtc tgatgtttat atctcttctg tttagttgag ctcagttaat
    18661 ttttgggcgt ggcatgagta tccattgttc cataatttat attaggaggt ttttaatctt
    18721 ttcgt
//
LOCUS       DAWDRC010000013        18676 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1601_length_18676_cov_2.805864, whole genome shotgun sequence.
ACCESSION   DAWDRC010000013 DAWDRC010000000
VERSION     DAWDRC010000013.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18676)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18676)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1601_length_18676_cov_2.805864"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5316..5325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4815 a         4468 c         4276 g         5107 t
ORIGIN      
        1 tgaggcagag ggtattgacg atttccgccg gttcttcttt accggtcacc aggccaagca
       61 gcagagcttt ttgcagatgc tttttcagcg catcccgtaa gttacgcgcc cggaagtgat
      121 taatggcact ttccgtagcc tgcggtaccc accactggat gaagttttga tagcgagtat
      181 agtcttcctg cgcctctgga taattaaaac cttgctttaa atcggcgtaa tgctggatac
      241 gtaacagggc taccgcggcg ttatacacat ctacccgtcg ggaaggatcg gtcaggtcct
      301 gctcagagta aaagttcgta ataatacttg aggggttaga tgccgctttt ggcagaaaaa
      361 tcaacgtaaa acgaccttct ttcagcggtg gtcgagaaga cataccaaac aactctggct
      421 gtgcatgaat ttcataacaa gcgttcagtg ccgtacgcaa atcacgggca acctcctgcg
      481 gcagaatgac attatcattc acccatgcac ggacagccct gataaagggt agttcggatt
      541 tggcagtgta ttttgcctct tctttcggtt cgaactccgg attgtcaatc tttttacccg
      601 ccggcgtact gcctgtcttg ccagtcgttt cgtgtgcgtt ctttgttact ggttccaccg
      661 atttcatcgt gggacctttg ccattcagtt gtgcggctag ctcatcgagg ccaaaggtac
      721 tcgcaacgct acttggcagt tttacgcgta gttcatcaaa agaatcaatg ccatatcccc
      781 agatggcggc aagtgtcacc gaactgccaa cccgactgac aaccagttgt gaaatcttgt
      841 cttcgatctc gggaacggca tgaattccag cgaaatttgc tggcgggaac tgtgtttttt
      901 ccgcttctat tcggcaatcg cgcagaatat ctaacaggat gcggttaatg attgtgcgcg
      961 ggttaaaaat caactcgccg ctgctgtcgc ggcaatgctt atccgccaat gccagaatgg
     1021 cactacggtt atacgggaat agcgggaatt tctgattggc agaataacca aaatcctcta
     1081 gtgcatcagg gatctcatca ctgtgccata caggtggcca cagagactta ccttgctcag
     1141 gacgtttttt taactccttt gatccaaagc gtgcggcgtt cagatagcga ccacagaacg
     1201 agacaatacg atcttcgtta ttgtctttat cgacgttctc aatgacccac tcgtagccag
     1261 cacgtgtgat taacgtttcc tggcggcgtt tatagccagg ataattgtcg gtgactgcaa
     1321 tcactgagtg gacaggacag agaacttgca cgccgccagg tgccgcttct tcgatcagac
     1381 tgtcgatcag aacatcttca atagcggaaa tcgccgccat atcttctacc agaatcacca
     1441 gacgacgatt aagtcgtaga aactccttgc ggatgtcctg aaaaagctcc tgaaaagatc
     1501 ctccattgaa ggtgaacagg cgcttgaaaa gtgatttcag cgaggtatgc atggcttcat
     1561 tgagcacgcg cgctgctcgg gcacgcgcct ccacttggtc tgactcatgc agttgagaat
     1621 ggttcagata gcggcgtgca cgcgcggaca gatcgtcagg ttcaaagata aaatagagat
     1681 cttccggaag aagttcatat ttatcatgat cttcttctcc ttctttcaga ccactactca
     1741 tccgactggc aagtttgaaa atacagtggc ctggattcag aagattctgt ttgaagttag
     1801 ggtcggtgat cagatcacag ataccgtttt cgacctcttc aatgatagct tccatatagg
     1861 cgagtcggtc tgcaggaatg tctttacctt gcgccagcaa tgtttgacct tcttccttga
     1921 ccgctttttg catgtcctgc aactgatggg ccatcatggt caacagtagg tctgccagat
     1981 ctttcgctgg gtactgttct gtcacttcat taacgcgcat gcgtgcgttg tcgaaaaatt
     2041 cccccgtcaa attttttagg atgcgtaaaa gcacctgccg tagactggcg tttttgggta
     2101 tccggacaat ttcccattgc tcattcttgt tgttttccgc taggtacatg ttgaatcggc
     2161 agtctaacca gcgcaccaga tgtgatttcc ccatccccga gcggccaatc agtgggatcg
     2221 cgcgctccgt atgaagtaac tgctggaaca ggtcttcatc acgcaatatt gtatgatttt
     2281 cattgacgtc gcgtcgggtc agtagcatcg gttcatgtac ggccagaagc acatgatcct
     2341 ctaattcttc tgcttcattg cgaatgcatt ggacgatatt tttcctggat ggccaatatt
     2401 ccattatgct cattctgcat cctctcttag cgtgatttgg ctaacccggc gaattttacc
     2461 gccaggtaag tgaagtgata aggcgtcttt atcatcactg tggatggaga ggataatgcg
     2521 tctactgatt tcaagtcgtt gcagcgcaac agatgtgctg gtgcagatat tgtcaccttc
     2581 cggtgcgatc cagtcttttt cctgctgcat cacgtcatga acggagtttc tgtactggcc
     2641 cccgtccatc atcggcatga cctccgccat gtttgccata aagattttgg cgggaagggt
     2701 atgcgtatcg tcaaagatct ccggcagaaa atattggatg gcccgggtag ggtcggtgac
     2761 ataatttccg tccggcatta actcgagaaa accgagaaaa cgaccaaatt ctctcactag
     2821 tttttcggtg ttgctattga tggtttcgcc aggtaaccat ttatgcactt tgttggtgat
     2881 gatcgtaggg gaaagaggtt cccggccccc gaccgagtgt tgctgaaggc tcaacaagca
     2941 actcagtacc cgccacaacg gttcgcaatc attcccactg gcaacgcctt tttcacgaca
     3001 agcatcaatc gtacagataa gcaaccgatg cgccagtgga cgcacacctg cttcaagcgg
     3061 taacatcttt ccgtcgagca tcggccagag tcccagattg caccaaaagc tcaggttctc
     3121 ttttaatttt ctccacgcgt tatcgttagc gggcaggtta tcaggacgat agcgggcgag
     3181 cagtgtgtcc tgagacaacg gttcctgtgc gctttgcaga acgcgttcaa tcaaaggaag
     3241 cagtatcagt ccgcttcctg ggtttgcgtt gttaattatt gacatgttgt agttgcctga
     3301 taaaagagtc cagcgacagc gcattagcgg cacttgcgca ccatgttcga tgtgggaact
     3361 gaggatcggc gagatgctca ggtgcaataa taattcgctc aaagcgcgtc atgggtagat
     3421 ctgggaacgc attagccgtg ttagggatga tcacgatctc tggtccatcc gatgcaggtg
     3481 ctgtcatttt ctgtgcgctc cagaaatgcg aaagcgaatc taggttgttc tctagttgca
     3541 ggaacactgc tctgtctgca cgaataaaca cgtattcgcc agaggtgatc agtgatcgca
     3601 acgtcacacg aagttccatc aacaacgaat gtaacttcat attttcgttg taataaactt
     3661 tcttttccgc acctacgaag agtgcttttt tcttcggctc ggaacgtaaa atttgtacat
     3721 ctgtgcccac ggtgggcgaa aacgcccttt tccctttctg gcgacatccc ggacaaccac
     3781 cacaggttaa ctcgggagga tggctctgat attcatagaa tttctgcaga attttacaca
     3841 gtggtttgcc aggatcctcc aaccacgttt ccattaccgt aaaggctgcc cggcggttat
     3901 tcaactcgga ctggcggatc ttctccacat aggtgttcca gtgcgcttct gaataatgcg
     3961 catcgtcaca gtgacggata tgtacgtggt tatagtagct ttcgaaccat tcaatcagtt
     4021 gcgtgttgtt gtcatccaga ttatctcgca ggtcgtcagg aattttcggt tcagcaaaga
     4081 ccagtttgat gagtccggct ctttgcatta acagcaacgt ccgacgattc caagcttcgt
     4141 ttttatcgcc ttggtgatcg atatgaacgg gcttggtgtt cagtgaaatc atgtagtcag
     4201 cgatcgggct tttcttacga tcgtcaaaca ttgctttcca gcgggcaaaa cctttttcta
     4261 cgccaatcag cgtctcaacg gccagcgatt ttgccgtttt catttgcgcc ttatgccata
     4321 ccagatgcac ctggcaggat ctgccgtcac gcccagcacg tccacagtcc tggtaatagc
     4381 ggtcgatatt ttccggaata gcagcatgaa tgatgctgcg gatttccttt ttattcatcc
     4441 ctaccccaaa ggcagaggta gcgaccatga tatcgagttg gtcatttttc cattttgaaa
     4501 tcaatttctt gcgattgttt gtgtcaattt gaccgtggaa tatgccgatt cgccagtagc
     4561 ccagttcgtt cagacgggca gaccacattt ccgcatctgt gcgggtagtg caatacaaaa
     4621 tcagcgggcg gggtaaggtc atcagaagtt gttcgacccg ctgacgatgc gcgttttcat
     4681 ttgcctcatg atgcacaaaa tattccggct ctgggcgtag gaaattagct gatacttcaa
     4741 tcagttcact gttttcatca tggaactgct cgaccagaat gtcgcgggta cttttgctgt
     4801 aagtcgccga aagcagcagc gtacgaaaac ggcatttgcc catggctttc agtgagcgaa
     4861 tcataggggc aattagctga aagtcagggc ggaactctgc gccccattga tcgatcaaat
     4921 gcgcttcgtc tatgacaatc gcgccaatca tattcagtct ggaaagctca aacagcaccg
     4981 gaaccaaaga agagcgaacg gattctgggg aacagaagag aacacgctgt gttcccgctt
     5041 tgattctttc cttaattgcc tgacgcgagg gctgatcttg gcctccgatc caaacataac
     5101 ttccaccatg atcttggtca gcaccgcgaa gcagtttttg catatcgtca gcctgctcaa
     5161 tcgcgagggc gacagtgggg acgatcacca gcgtgagggt gtttgtcggt gcgctgagcg
     5221 cctggagttg ggtgagcaga cttttaccgc aactggtggg cagattaacc agcaatgtac
     5281 aggagttgtc acagtgccag aaaaatacac cacggnnnnn nnnnnattat cagacgttag
     5341 cagcgtccgg atggcggcct gttgaccgaa tccctgatag tgagtcagcc atggctcttc
     5401 gggtaacagg cgcctcagtg ccggatcagt ctcggggcgc agcaagttcc tgcgtatctt
     5461 acgctgatag gtggctctgg tcagcgccag gtccgactct tcagccagtt gaatttgttg
     5521 cacggcaggc aactttgtca tgccccactc aggtgccgga gcatcggtca gttctggcgg
     5581 caggggtagc cacggctcgc taataccact cgcgtgagcg gcacgtaacg catcggcgta
     5641 gccggcaaaa atgtcaccat gacctgccga ggcatcggtg atgatctgaa tcagtctgtt
     5701 cagaaactga ttttcgatgc catctttaaa ggtgacccct tgactaaaat aggtggttag
     5761 cgcctgctgc aggcgtgacc attcctcttc catcagacga tttcctctaa tagatttctg
     5821 ctgcacacca ccacggcttt cacggccatt aactgaagtc tggcttgttc gccgaaggtt
     5881 gcctgttgtt gttctcggtc actggtatag actcgttcca ccatctcttt ccacgtgtcc
     5941 ggtgagacaa gttcttcgat ttcttgccaa agattagggc gcagatttat atctgcatag
     6001 aatttctgcg atgtttcatg tttggcgtac tcgccattga gcagttccag caactgcgga
     6061 tttatcaccg gcgtaccatt agaggacagc cagtgttgca caattcgcgg cgggtacaac
     6121 tcgtctgcaa tacggcgttg tgcgtaggta tcctgcgtct ggcaatgggt gacaagccaa
     6181 gttgactgaa agaagtaatt gggctcattc agtggcttgg ggagcactcg cagtaccgcc
     6241 atggaactgc ctcgggcatc ttggtttaac agttgccaga tcgtatcgac aaatggctgc
     6301 ccataacgca gcgggtaaac ggttgttccg tcgatcgccg gaatgcgttc agcgctcatc
     6361 agatgcgttg acggtgggtt gcccccttct ttatcgaagc tgagcatgca ggtggtgaga
     6421 aaggttttaa aatcgaccag cgtccctgtg ctgcgatcag agagttcgaa ctgaaacttg
     6481 ataccttttt ctgcagtgcc ggtggtgcga aaacgtagca cacgcgtgat ccaacttgtc
     6541 agtgcattac actgcgctac tgcaagatga tcgtcagcgc tgatctgttg cgcaaaaatc
     6601 gccgcctctg caatctcatc atccatcgcc agcagctttt cctgcatgtt aattttatgc
     6661 gtttcattgg ccagaatgcc ctgttcgcca gggaattctc tggcaacacg ttcaagggcc
     6721 accgggcctt cttggtagac attttgccac gcattttcta gataatcgtc gaggacgaac
     6781 tggaggctgg cgattgtgtt attaaatacg ccaatatagt ctttcagaat gcgggcccac
     6841 acgttgcgta atccacagtc ggcgtgattt ttcaggatca gactctggac aggttttacg
     6901 cctttcaggt ttgcactgta cctgttcaga cgaccaaggc gctgttcaat acgactacaa
     6961 gagcgcggta aaccgtagtg aacggctagt cgcagccccc catggagatt cagaccatct
     7021 tcgccgcttt tatcgcaaat taggatccga atagggctgc cgggtttgag gtacatcaag
     7081 ggatgacccg gctgatagag ttcctggggg tactccagca gtagttcgag ctttattgcc
     7141 aagtgttggg caagatccgg acgatcgcag aagacgattg cttttcctgt cggattcttt
     7201 tgcaaccact cattcagtgt gatggcaagc ttgtaatcaa tggcctgtag ttcatttttt
     7261 gcagttgaaa caatgtgctc taacagatct cgttcttcct gagtgcgttc cgcttcaccc
     7321 aaatgtactt tagcgcgttg cataacacag agtgggctgg ataggagatc gtcaacccag
     7381 aagaggtacg tgtcttcact cattgcccac tgcgtattct gcggcagtaa agataatgaa
     7441 cgatactcat caatcagttc gtctaatgct aacccatcgc aataccagcg gcactcctct
     7501 gctccattta gcccaggaaa cagaaggttt agattgaggt ttttcgtttc atcgaagctg
     7561 agtgaaacct gacggcgatt acgcaacagt cgatgatgaa tgcgatagtt ttcccccaag
     7621 tgccggcgaa tatcccggat cccttcttca cgctccggcg tattggggga gaacgtccca
     7681 ataagcggaa tgagttgctg gcagagcgtc atcagaacag tatcttctgg gaactgctgc
     7741 tgcagtgttt ccagattacg acgtaagctg acgtttgaga cggaaggtac taaccccgag
     7801 aagatcccgc caaggttttc gcgatttttg actagttcgg taaagtcttg cagatgggtc
     7861 tcatccactt ggtaggcttc cggattaata cagtgcagca tcgacaagaa gttcctttca
     7921 tgcccatgca gaggggtccc cgtgagtaac agaacgtagt gggctttccg gcacccctct
     7981 gcgatcaggt tgaagtcata acgttcatct ttatctcctg accacggcca agaggcaatc
     8041 tggtggacct catctactgc gataagtgaa ggggtgaatc cccgttcaat cagggtacag
     8101 ccatcgcaat gactgcaaat cacgatgcct tccgtggcct cattttgtcg caaacctgcg
     8161 agggccgtcg aagcatcaat aataagttgt ttcagttgaa aacgttcagc cagttcttcc
     8221 cgccactggc taactagtgg tgccggaacc gcaataagca tgctgacgtg gccagttgct
     8281 tctaacgcgt gttcacggat gaccattcct gcttcgatgg ttttccccag cccaacctca
     8341 tcggccagaa tatacttggg gtttttatcc tgaagtacac ggcgtactac cgccaattgg
     8401 tggggttcga gttcaacggc actactagaa atggatgcca tcgcgcggca tgcagcgcgt
     8461 tgctggatgt aggtttcaat aaaggcttgg cggtaaggga aaaagaacgg tgcgtcatta
     8521 gcttggaggg caaggaaatc tgcagggttt atcggggtat ccggcgcttt gttcagaaca
     8581 aaaatttcgt cgattggcag cgtcgtagtt tcatcttcgc gaaagatgac gagaaaatcc
     8641 cctttggggc gcgatccgcc aaaccgggct ctagtccatc gttggctatg tggctcaatg
     8701 caataaatta ccgtctcttc gtgcgggatt gtcagggtga gttgttctaa aggaactttc
     8761 atttgtcgtg cataaggttg agccggtgac tcaaaaaaag cgaccgtggc gttatttgta
     8821 tcactatcga cggtgacaac tttaccgatg ccttcaaatt gctcagagat aaccagtgac
     8881 ccgatgggta atacgttcat cctcacatcc ttgaattgtt tcttataatc acatttgctc
     8941 aaaaacaacc aaagacagac cgaaaatgtt actttgctat gcctgagcga catgcttata
     9001 agtgcattga tttgagtatt atgatcagga tactgagata attctcatat tcaaggggga
     9061 aagtagaaaa agatcaatga gatgttggct tgtgtagggg agatttcagc gatatcaatt
     9121 ttatctgtta caatgagtat ttttcaatct actaatgtag gctggatgaa ttcaaaaata
     9181 attgttaagt aataataaaa tgatcaccat cgaatattaa atattcatat caacgccact
     9241 aaggaaaaca taaactgccg agaattgtga cgcaagttat aaaatggaag gttgtttgtt
     9301 gccatcgact aaggaatgtg actggtttta tccaaaccag tcatcgggtc ggatatcatt
     9361 caccgcgata aatgcaaccc gctgtgcagg tttctttcac cattacagcg gtcaggtgtg
     9421 gcaactgtga tttaatctgc ttccaaatcc attctgccag tacttcgctg gtcgggtttt
     9481 ccagaccagg aatatcgttg aggtagtagt gatccagtcg gtctagcgtg ggtttgaaaa
     9541 tcgccttcaa ctcggcaaaa tcgattatcc aaccggtgtg tttatcgacc tctccggtaa
     9601 tttccagacg taccacaaaa gagtgtccgt gcaaacgccc acatttatgc ccatcaggca
     9661 cgtttgggag tcggtgtgcg gcttcaaagg tgaaatcttt gtacaatgtt gttttcatag
     9721 cgacctcgtt ttttgttata tagtaacaac aagaagcaag acttcctaga atttatgact
     9781 tagtgattaa cactactgta ttgtgtagtg gtcaacaaaa actggtcaca atttcagatt
     9841 ttttccagta tctattttcc gattcgttcg gaggtaatcc accgttatat tcatgaggcc
     9901 ggagtgcact taatattcaa ttatatactc tgttattgta tggacacctt cgttaaggct
     9961 aaagtaacca gtcactggtg cccactcgtt cttcagactt ctgaagaaac gtaccattag
    10021 gctaatatcc cagcagtttc cccgacgact catactctcc ttgatccgat aacgccacat
    10081 cacctgcctg aactgtctac ttgtgtaatg gctctcctgt tgctaaggaa cataactccc
    10141 tctggtttac ctcgcgtttc ctcggccatc ttcagcgctt ttatagtcag tctgctgtcc
    10201 ggtgagaacg acattgccca tccccgggct ttattgcgaa cagagcaagt actactgcga
    10261 ggtatcccca tcgtttacct atccagatat acgtcacgtc atcacacttg atttagctgt
    10321 gtcacagcga agtgcggttc aagatagtta ggaatagcga catgttcatg accggtgggg
    10381 cggcggctga cagttgacca gcccctgttt tttcatgagc ctgccagcaa gccagcgtcc
    10441 catctggtaa tctctccggg ttgccattgt ggcaatgctc ctagctccgc catgcaagtc
    10501 aattacctag cccatctgcc gtctgttttt tcaggacggg ttttccaata tctgtagctg
    10561 cagcgatgaa ctgcgaacac atgacagagt gtgaacacag gataatgcgc tctgagtttc
    10621 ccgattattg agactgttca ggaatctgac atcaagagcg cggtagcctt cctgagaaac
    10681 tcctggttca ctctcataat tcagggtctt gctggagaga ataacccagt gattttactc
    10741 ttcgtttaag tccattgagt acgcaactac ggtaacgctc ttcatagtaa tcagcacaat
    10801 tgtcgacata ttttttacca tatttcaagg cattgtagaa cagtgttgcc agttttcttg
    10861 ccgttgcagt gactgctttt gctttaccta tacgggatga taatcgacga taaaaagcac
    10921 ccaacgcagt atcgctccga ccaatcgttg tggctgccag cctgagtgca gcagcaattc
    10981 aactggatga acgtctcgtt ttcgaggaca acacttttcc accggagatc ttatttcccg
    11041 tggacaaaat agccacgagg taaaatggct cgcatcaggc cagcgattca tgtcagtacc
    11101 gcgttcggca acgaatttta gtgccagata aggcccaacc ccatgtattt gtgtcagatc
    11161 tgccgtggta attttccaga gtaacggtct gacatcaagg aaagctgatt gggttgcttt
    11221 gtacgatgtc tggcgcaggg taatactcct tcaggatttt cagtcccggt gctgagttgt
    11281 attaatgaat ttcgatttga tcgtcacgtt cacggatctt ttcctggtaa aaatagaaaa
    11341 atgccaccga ctgctcaagt gcaaaaagat gctcaggttg ccagttccct gtcagtgcct
    11401 gctgaagtac ttctggcgtc ttcttacatc tcacatcacg gtactggatc aatactgaag
    11461 gatcgggctc accgctgaca atagctctga cgatcgagag accggtgacg ccagtgatat
    11521 ccgatacggc atgatgcaat tgcacattca tctgcatcag cgctttctgc atatgctgaa
    11581 tatgcgcagc ccggtattca gtcaagcgtt ctcgttgcct cagataagac ctgaggactg
    11641 aaatgtcgtg tacaggtctg aaactggccc ggagaagacc aaatgcatgt cgtctctgca
    11701 accactgtgc gtcgttgaca tcagtcttgc gaccgggaac attttttgcg gcactgacat
    11761 taacgagtat catattaaaa cccgctgctt caaggatctc aaaagcaggc aaccagtaaa
    11821 cgccggtgga ctccatggcg acggttgtaa tatgacaggt tttgagccag tcagacatac
    11881 gatgaaggtc gccggtgaaa ctttgaaaag tccttattgg ctcggcgtca gcatcaggcg
    11941 gtacggccac aacgtgaaat ttggccccaa tatcaacgcc cgcagcatga gaatggacaa
    12001 tagacattcg ggaggatttt ttcatataaa tctccttgct catttttaat gaacgaagga
    12061 ggagacgacg gagaaatcac cttcctgaac gggatcaagc taccaccgtc ggatccgcca
    12121 cagcccctga accatgtttt tttaagggtc ataccaccaa aaggccgacg actgcttcct
    12181 tcgctatatt agactgccag ctttaccggg gagtttctga gactcgggac ggggtttcac
    12241 acatggaaag ttttttaata tttcgtttcc atttcaattc gttgtagctt tttctttagc
    12301 tcacgtagtt cgatttgttc cggtgttatc aggaaggctt ttggcgtttg ccctgacgct
    12361 catcacgcag ttgtttgaac cattccgtca tcgtggaaag accaacatcc atagccttag
    12421 cagcgtcaga aacagtgtaa ttttgatcga caaccaactg agcggattca cgtttgaatt
    12481 caggactgaa atttcttctt ttcattggtg cacctgtgtt gttctaaggt gagcatatca
    12541 ccctgttcag atggccaaat tcagtagacc acttcagccc tgaattcgac ttttacaatg
    12601 acttatctgg acgcatgaag aacgtcagga atataccaat aacaagcaat gcgacggaac
    12661 cgtagaatgg taaactccag ttgcctgttt tatcgataat aatgccaaag gcgataggtg
    12721 aaataatgtc ggcgacagcg gaaccggcgt tcatcaatcc gctggcaatt cccacatact
    12781 tcggcgtaat atccatcggg acagcccgga taggccctat ggtcaactca tcggccagca
    12841 atgtcgtgaa ctgtgagacg aaacagcgta cacagtttga atgtatctac ttttcccaat
    12901 actgggcgaa gggagatgtt attgcgaatc gcgcgcctat tggtcagtgg gaaccttatt
    12961 cggaagagtc actacttggc atcatcgtca ccagcgtctg tcgtattaaa gtcgcgatgc
    13021 taaaacctga acctcccaga gatccccata tcccgctgat gggggatttt aactaaaact
    13081 tagcgctatc gctgcgtatc gtaactatcc cgtattacgt agcgtaaatt atgacgccat
    13141 acggggaaca aatacgcaga ggaagaaaaa gattagattg gttttaaggg atgtttttat
    13201 cataaatggc actgctaaaa ttatgagatt atcgaagata aaattcttga tcatacataa
    13261 aaggcctcct gaattaaatc aagaggcgta tagtttttac attactgatg tatgtttaat
    13321 gatctcactg attttcactt tcctgtcctg cgatcttgaa agagtggcag catcagcagt
    13381 agcaatcgcc gacatggccg cctcaccatt gagaaggtca atataatctt cttcaggttt
    13441 tgcaccacag aggatattat ggaggaataa cgtttccttt cttattaaac tggcaagcca
    13501 taatggtgtt ttttttcctg gatgaccata tgctatagcg ccatccattt ctgaggtcat
    13561 attgcctttc cgacgatcat catcttcttc ttgtgtttca tggaccaaaa aatgctttgt
    13621 ctgaccgcca atcctaagtg accctgctgt ttcttgcata tcaattttaa tagagccttt
    13681 agttccattg atgatgacat aatgttccgg ccagttaaat gcactccccc actctaaggt
    13741 tgctagtttt cctgacggga attccaaggt cataaataac atatcatctt cattgccaaa
    13801 tcctggacca gaatgggcca aatttccacc aatcatagta accgtctctg gtatttctcc
    13861 aagtaaatgc tgaacacaat ctaactcatg tatatgatga tatagatgtc caccagattg
    13921 ttctttcatc tttttccagg aaagtctctc ttgtttgttt tcccagccat ttctcttagt
    13981 atgacatgat aatatttcgc cgataacacc ttctttaatt aacttccgtg catattgaac
    14041 cccattgaaa aaattcataa tatgcccggc cataaaggtc acaccagctt ctttacacgc
    14101 tttgaccata tccacacaat cttcataact taatgcaatt ggtttttcac aaaaaacatg
    14161 cttcttattc tttgctgctt taattactgg ttctttatgc agataatttg gggtggctac
    14221 gatcacgcaa tcgactaatt tacttgagac taaagcatcc aagcttgaca tattgataca
    14281 ctgcaattca cgggcaatat tttctccatt ttcaggatcg tatacacatg taatttttgc
    14341 attatcatgc atattcataa aacgagctaa ttcagcgcca aagtatccaa caccaacaac
    14401 gccataatta atcataaagc ctccaagcca taatatcctg aaatgtatgt agctaataca
    14461 ggtgcggcca ttcctccagt tgcccctaag ttataaataa gaccggtccc taatcctctt
    14521 aattttgttg gaaagtaatc atatataaat tttggaacca accctgcaat acctaaattt
    14581 gtaaacatta atccaaagag acataatcct ataagagaag agtttttcac agaaataaaa
    14641 aaaagaggac aaaggaaaac aaatgaagtt attagaccga ctacaaaggc tttttttaca
    14701 ccaatcttat caccaacaaa accaaaaaat attgtacctg tcagtgttcc taaacctgct
    14761 attgtcatca gagttgaaat aaccactgta ttaactccat tatctgccag gtaggaagga
    14821 agtagtccgt ttatcggcca gtttgcacca aatagacaaa aacagacgag gaaaacgatc
    14881 atagagattg aaagatgtgg ttttctgaag acagacaaaa aagttgattt atccttatat
    14941 ttatcttcaa tccactcctg actttctgga gcactttttc tgatccaaag aactagtaaa
    15001 actggtaaca ggcctataaa aaaagagttt ctccatccat atacttcagc aaactgaggg
    15061 attatttgtg ccgcaataat atttccaaca gaaaaaccac ttaccaaaaa agcactagct
    15121 ttagattgaa gatttttagg ccaactttct accgcataag ttgaagcaca tgcatattca
    15181 ccagacatcc ctaagccaac aataaaacgg caaactgcga gcatatataa gtttgtagca
    15241 ataccgctaa ggcctgttcc gactgagtaa atgataattg cccacatcat cattggctta
    15301 cgaccatatt tatcagccat ggcaccaaaa aaaccacctc caataggtct ggctatgaag
    15361 gccactgtcc ctattaaagt agcctgaata tccgtaatgc caagatctgc ttttataata
    15421 tgaagaatgt aaaatatcat cataaaatca aagccatcaa ctacatatcc aagccatgcg
    15481 gaaaaaagag ctttccgttg tggtggatta acttgtttat accatgctgt tgccatattt
    15541 gtttcctgtg ttattctaat atagccctta aagtaaatat tagggagcct caacaatatc
    15601 cagaaataat tatattgtct tcaaatacgc cagtactatt gattgttatc aatacctaat
    15661 ccgtacaatc ttttcctcca tttcttctta acatatttca ctctatgaaa gataaaactg
    15721 atacccacct acagttttgg gtcaccaaca aaaaacaatg ttcgccagat attccaccac
    15781 tggcacagtt caaccctgct tcaaaaattg acttattcat tgtgctcatt ttcgtagtcc
    15841 gttcaggttg aataaaactc ccttagtcaa atgaggaaag actgagaggt tattacttta
    15901 gcttacgtta tagctgtttt cctttgcagt atttctttgc ttttctgtat acctttatac
    15961 ctgttatacc agatcaaaaa acaagcaatc cacataacaa aacgcgtttt gttactgatg
    16021 tcacaaattg agcatatttt tgtagctgat agtttgttac aaacaccaga accaagatgc
    16081 ctatttcgtt tcagcatcaa ctgttaaatt atctggctgc caacgagaat actgagcttt
    16141 tttgtcccca agactgaccg tcaacgccac aatctttgac tgtttgacat tgtcatgccc
    16201 gcaggccagc ggatctaaag acattagact gcttgcatca gccagttgcg tatctggcta
    16261 tgtcccttaa ttggtaaggg ttcgaggaac tcaacattaa cgcatttttc agcgcatcac
    16321 cgacagtgta tatgaacgat tgcactctga cgatggttga tttatttggt tgcgtagggg
    16381 ctagctagtc tggtaccgga ggctggatct gaagtactga ccccaggaat aacgccgggc
    16441 tgactcatat tctgacccat tgtcagcccg cgtgtttact gctctagctc atttcctgtt
    16501 gtcaggcgtt caattcccaa ctgcaggtta tggcccggtg cacaaacacc tgacggcagg
    16561 tatcacttac ccgaagaagg taaagccctc aagaggcagt aactcagcca acctctcctc
    16621 cggccactca ggcagacgcg tcaggacgtc cgtcagccaa gcatgtggct ccagactgcg
    16681 gttcccagaa ggctcattat ctgtgccgcg cggttcccgg ccatcagtga accagcgaac
    16741 aaccaggcct ttcgtcccat aacgaccgga cggatagcac gctcgcagat attgttgtgt
    16801 gtgccttgaa atgcacgatc tttccgtcgg tgaaagtccg ccccggctaa ctctcgctcc
    16861 gactggaagg acaataagcg gtccagaagg taacaaaagg gctgaatctc tccctgaaac
    16921 gacttcttca ggaagtcaac gggttatgcg ggccgtaacg tgagtgaaca ctgagcagcc
    16981 atcgaaatgc cgtcgagggt gctgacacgc cacaataacg gggaaggccg acgatgaccg
    17041 ggaagagagt gagaaaggca tcggtcatac ccaccggggt tgtggtgtca gcatgcataa
    17101 caattgatcg atcgtgcaac aagagaagtc cataacgatg tctgtccggg tgacgggcaa
    17161 caacgcctcg cgagggggtg aatcggttgt tatggatggc agatggggtg tagtagtgaa
    17221 aatactgagt aatgtcagag gagcgaaggc cccctcttgc agtaccagtg taacatgcag
    17281 gaagagcccg gggattgcca tcagcctcac aaccccgctc gaaccggttc agaacctaca
    17341 gaaaacacta caggagaaag caaagtgaac cgccccgtgt ttcctgttta tcattttctg
    17401 gtcagtgccg cgattctggt attcgtggtg attttctggc ggacacacca tcgcgaccac
    17461 cgtaactggc tggctctacg gctattcgta ttatgctctg taaaccgttg gccattgcgg
    17521 atggttaaag gaacagttgt ggggacaact gacacattac gtgaaatgca gcgttatgaa
    17581 caactgagtc agcacggggc tgacaactgg aaaatccagc cgggagcacc gttatatgat
    17641 acgattgtta ttgtcactgg tgagagtgtg cgcagggatt atatgtcagt gtatggctat
    17701 cccgtaccaa ccacaccgtg gctgaatacg gcacccggtt tatttattga cgattatacc
    17761 tcgacagcct ccagtacagt gtcttccctg agccggacac tgatttatga ctatgagcag
    17821 aaccctgatt ccggcaacaa tgtggtggcg ctggcagcaa aagcaggata cagcacatgg
    17881 tggatattca atcaaggaaa actgggggag catgacacac gcatctctgt tattgcttct
    17941 gatgcggagc atgccacttt cctcaagaaa ggcagcttcg cttcccgtaa aacagatgac
    18001 aaactgttgt tacaggaaac agaacgtgcg ctggcggata catcctctcc gaagataatt
    18061 ttcctgcaca tgatgggttc tcatccaaat ccgtgtgaca gccttaactc ctggccgaat
    18121 aattacctgg agcagtatcc ccgaaaaatt gcctgttacc tcgccagcat cagtaaactg
    18181 gataactttc ttggccagct tgatggtatc cttcgccggt actcacgtca tttcgccatg
    18241 ctttactttt ctggccttgg gctgtcggtc agcgacagtg ccaatcctgt tcatcattat
    18301 ggtcatgtgc agggaggcta cagtgtgcca ctgattatta ccgccagtga cataacgtct
    18361 catcagcccg tcagtagaaa aatcagtgcc cgtcatttcg caggtatttt tcagtggatg
    18421 accggtattt gtactgaaaa tataccgcca ttcaatccgc tgacagacga agataattaa
    18481 actgttatgg tttttaaggg agagaggaat ataccggcag acagtttgaa acctcagcca
    18541 cttattcttc ctgatcacag gtaatcatat atggcagact gtaatacagt cgcttgtact
    18601 gaaaagtatt ctgtataaaa tatccgtatt catatcagca caagggacca tcaaaaccct
    18661 gcagcacccc ttacgt
//
LOCUS       DAWDRC010000014        18158 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1673_length_18158_cov_2.245208, whole genome shotgun sequence.
ACCESSION   DAWDRC010000014 DAWDRC010000000
VERSION     DAWDRC010000014.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18158)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 18158)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1673_length_18158_cov_2.245208"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5638..5647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6255..6264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10474..10483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12722..12731
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12964..12973
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4346 a         4968 c         4592 g         4202 t
ORIGIN      
        1 tcgagataga tcgccagatg atctgccggt tcgttgaaat tgccgctggt ttccatcccc
       61 gcctcaacta acaagcgttt aatctcttgc tcgtcctgtt tgtaggccga tgcatacggc
      121 agcgccgctt gtttgtcggt catcagaaac aggccgcaaa agtcagcggc cagttccaga
      181 cgggcatcgt cacgtactgt cagagtggca atactgtttt ccagcccatt caccgcctca
      241 gtgagcggag gttcgctttt cagcaacgaa aaccattcag ccatctgcgc actggcgatt
      301 tgcgtcagtt gttcatcgtc cagttcatgg gagaacaact gcgccagcca ggcgtaaaca
      361 caggcaatct gttgtgctgt cagcgtggtc atgatttcac ctgcgacgcg ggaacatatt
      421 cgcactgcgc caccatctcc acggggccgt taaacgccgt cacctgctcc actgttccgt
      481 tgtacttctc aatttccacc agcgtagtgt gcgcactggt cgcctgcgcc agctgcgaag
      541 taccgatgtc gatggtcaac acgttggggt taccgtattt gcacagcgcc cctggctcgc
      601 cgcctttgtc cggatcgtac catgcccctt cgtgaatccg cgccacgcca ggtgcatagc
      661 ggtcagaaac cactgctcca gccaacacct gaccacgaac gttaaagacg cgtaccacat
      721 caccgtgacg aataccgcgc gcgctggcat cctgcgggct aataaatact ggctctttac
      781 ccgctacggt atattgctga cgcagagttt ccgactcaca taactgcgag tgaagtcgga
      841 aatccggatg cacagattgc agatgcaacg gatacttctg cgagccaggc ccaccgtggg
      901 agcgttcgat tttctcaaac cacatcggat gcccctgaca atcgtcgtag ttcatgtcgg
      961 cgatggtttt cgagtagatc tcaatcaggc cactcggcgt gcccagcggt tcgagatccg
     1021 gatcttcgcg gaatgcctgg tggcgaacaa acatctgcgg atggtcaaac tcgacgtact
     1081 ctttgttatt ccagaagtca tcaaacgctg gcagatgaac gccgcgtcct ttgccttgtt
     1141 gtacaccttc ctgccagatg cgtttcagcc agcccatctc gtccagccct tcggtaaagg
     1201 cttcttcgcg attaaagcgg cggcacagct cgcggaaaat atcaaagtcg ttgcgcgcct
     1261 cgaactgcgg cggcacgacc tgtttcatgg cgatgatgcc acgattggag tggttgccgt
     1321 actggtcgag atcgttacgc tcaaactgcg tggtcgcggg cagcacgata tcggcaaagc
     1381 ggcaggttga ggtccactgg ttatctatgg cgataaccgt ttccagcttg cgccagcctt
     1441 caataatgcg gttgatctgc tgatggcgat ggaacgggtt agttccggca aaaatacaca
     1501 ttttcagcgg cggcagtttt accgatttac cattccagtt gatcactttt cccggttcga
     1561 ggatcgcatc gacaaaacgg gcaatcggaa tagtgctgct atagccttgg tagtcgctgt
     1621 tgtcgtgaac aggtggaatc gacgtagaac cggagaaacc actcagaata acgcctttac
     1681 gccccggcgt gcctgcgccg ttatagtgcc agccaaaacc gaagccacca cccggcaggc
     1741 caatttgccc cagcatcgcc gccagaacga caatcatcca cgcccactgt tcaccgtgct
     1801 gcatacgctg cacgcaccag ccagcaataa tctgcgttct gttcgccgcc atctgccgcg
     1861 ccagcccacg aatggtttcg gcatcaatgc cggtcagttt ttcagcccat gcggcatctt
     1921 tcggctgacc gtctttctca cccagcagat acggcaggaa ctgctcaaaa cccacacagt
     1981 agttagcgag gaagtttttg tcgtacaggt tttcgctgta cagcgtatgc gccagcgcca
     2041 gttgcagcgg cacgtcagtt tgcgggttaa ccgcaatgtg cttcacatgc tcgcgcccca
     2101 gatactcatg ggtggatgta acaaccggat cgatgctgat gacctcaatt tcaccggcgg
     2161 cgactttcgc tttcaactgc tcgtaatatt cataaacatc gtgatccggg caccaccagt
     2221 tcgcctgctg gtttttcagc aaatcggagc cccacagcac aatggttttg ctattctgca
     2281 ataccagcgg ccaggaggtt tgctgttcat acacttccat tgagccaacc acgcgcggca
     2341 agatcacctg cgcagcaccg gtagagtaat ctccgcccgt accaacgcta ttaccatgca
     2401 aggcaatagc tttcgccagc atccccgaag cgttatggaa catccccgtc gattgccaac
     2461 cactggcggt cagcaaggca ctcggcccgt gagttttctg tacgcgttcc agttcttcat
     2521 agaacatgtc gagggcttca tcccagctca cgcgcacaaa acggttatca ccgcgctggg
     2581 aggtgtcgct cagatggcgc ttacgcagcc agtccacgcg taccatcggg taacgaatac
     2641 gcgccgcgtt gtgtacatga tccggcaatc cggcgatcat tttcgacgga tatttatcca
     2701 gttcgaacgg ttttgctgcc acaaagcgac catccttcac cgtcgcgcgg atagcccccc
     2761 agtgcgaccc ggtaagaatg ccctctttcg agatgacagc ctcagtcgcc gcttgcgtcg
     2821 cagtcgcccg gcgcggcgtt aacaatgacg gccccagcat cccggcgacg gttaagccgc
     2881 cgagttgtgc cagaaaacgc cgacgtgatg cctgaaagag atcgttattg ttcattattt
     2941 ttcttccttc ttatcgccgt gagctttacc tgcggtgtca gacgcattca tttgcagata
     3001 tttcaacaag gtgcgttctt cacgtttatc gaggctggta aagccaatca tgccgttgag
     3061 cgtgccgatc caaccgttag cgtcaaagtg ggatatttcc ggtgcgccgt ggcactggtt
     3121 acaggtgccg ttgtacagcg aatccgcata agcccagatc ggtttgatat cgttcaccat
     3181 gtcgcctttc ttcatccacg cagtggcctg caacttgctc cactcggtat tggtgtcggc
     3241 aacggtggtt ttctccagtg ttttcacctg ctgctgcaca tcaccacgaa tcgaggcaac
     3301 aaagatgcgt ttacctggga attgggtgag tacacgctga cgtcctgcac tttccgtcca
     3361 gccagtgatt tcaatttgca gccagtcgcc gtcacgttta aggactttca cttccgaagc
     3421 aggcagcaga gaaccagacg cttctttatc gcctttcgcc gcataaatcg gcttaatatc
     3481 aatggagtac agcgtgtcac cactatcatt agcactggcg cgcagctcat cgaactgctt
     3541 acggaagcca ctactcatat ccggtaactg gtgggcaata cctttatgac agtcgatgca
     3601 ggattgatta tctttcgctg ccaccttcat ctgacgcgcc gcttcaggat gctgcttcgc
     3661 atgatccatc gcatcgtagt tatggcagga gcggcaggtt gccgagttgt tttctttcat
     3721 tcgcgcccat tcacgctcgg caagttccgc gcgtttggct tcaaatttct caggtgtatc
     3781 aatggagtgg gcaataaagg tctggtaaat gtcgttgctc gcttccagtt tgcgcttcac
     3841 catgcctgga atatccggcg ggatatgaca gtcatggcat tcagctcgca cgccggaggc
     3901 gttctggaaa tgcaccgact gtttatattc ttcatacacc ggttgcatac tgtggcaact
     3961 gacacaaaat tcggttgtgc tggtgacttt gatcccaacg tgtggcaata caatcagcgc
     4021 aatgccaatc acaatcccag tagcgaccag cgccagtacc gaccaacgag cactgggtcg
     4081 gcgtagcgcg ttccagagtt tccgcataat agcccctgta aaattatggt ttagtgaagc
     4141 gatcttaatg agcaaatatg aacagcggca ctggtcagga tgaacggctt acggcagaat
     4201 atgaacagat atgaacagaa tgagtaaaac cctctgatgc cacatcacat tgttattgtt
     4261 gaagatgagc cggttaccca ggcgcgatta cagtcttact tcactcagga ggggtatacc
     4321 gtttccgtta cggcgagcgg tgccgggctg cgggaaatta tgcagaatca gccggtggat
     4381 ttgattctgc tggatatcaa tttgcccgat gaaaatggcc tgatgttaac ccgcgccctg
     4441 cgagaacgct caacggtggg gattattctg gttaccggac gcagcgatcg gattgaccgt
     4501 attgttgggc tggaaatggg cgcagacgat tacgtcacca aaccgctgga gctgcgcgaa
     4561 ctggtggtac gagtgaaaaa tttgctctgg cgaatcgacc tcgcgcgaca ggcgcaaccg
     4621 cacactcagg acaactgcta tcgctttgcc ggttattgcc tgaatgtgtc gcgccatacg
     4681 ctggagcggg atggcgagcc gattaaactg acccgcgcag agtatgaaat gctggtggca
     4741 tttgtgacta atccgggcga aattctcagc cgtgaacgtc tgctgcgtat gctctctgcg
     4801 cgtcgggtgg aaaaccctga cctgcgcacc gtcgatgtgt taattcgtcg tttacgccat
     4861 aaactcagcg cggatttact ggtgacacaa catggcgaag gttatttctt agccgctgat
     4921 gtgtgctgat aataatagac cggacgaaat ccccccggtg acagcgagcg gcggatatgt
     4981 tcgcggtcgg catttttcgg cgtcagaact aaaatcggtg ggctgacatt atcagacacc
     5041 gattgcccct gtaattgcct gatggcctgc tcaaccgcca gttccccctg ccagaccatt
     5101 tgatcgctgg cagccataat cactcttccc cgcttcagcc cgcgatacac ctgatgtgaa
     5161 agataaaacg acaccacggt aagcggtgtt ttcaggttac gcccttcccc cattgccgcc
     5221 tctgccgcaa tggccgttcc ggcaacgacg tcgatttctg gatggcgttc cagcatctcc
     5281 tgcaacaggt tacgctggat ttcaatatcg ttatcaccga gcgcaatatc aacaatacgt
     5341 accgggcttc cggcaatggc tgcgcgaaaa ccctctacca tctccttact gcccccggcg
     5401 ttatcggggc cgggcatcaa cagcacgttc agtggtttac cgtgcgccca ttgcaccaga
     5461 tatcgccctg gttgatagcc catctgaaac cagggcacac caacgcggct tttcacctgg
     5521 ggagcatcaa tagcattcac cagttcgatc accggcagat ttgctacctg cttttgcaga
     5581 tcgggaaatg acgtcgtgct actaccgagc aaaatggcct ctgcgcccca ctgtttannn
     5641 nnnnnnngct tgcaccgtgt aatgctgcgc atcatgccag cgcaacaggt tatcagccga
     5701 aaatgccgat aacatgaaaa gggaaagaag taaaaatagc agtacgcgca tgatagcctc
     5761 atcaataata aggctttatg ctagatgcat tctgctttgc gactcaacct ttttcaccta
     5821 aagtgcaccg accgtgaatt taaccctgac ccgaagactg tggatgggct ttgccctgat
     5881 ggcgctgtta accctgacca gtaccctagt gggatggtac aacctgcgct ttatcagcca
     5941 ggtggaaaaa gacaacactc aggcattgat tcctaccatg aatatggcgc gccagttgag
     6001 cgaagccagc gcctgggaac ttttcgccgc gcagaacctg accagtgccg ataacgaaaa
     6061 gatgtggcag gcgcaggggc gaatgctcac cgcacaaagc ctgaagatta atgcgttgct
     6121 gcaagcgtta cgggaacaag gttttgacac caccgctatt gaacaacagg agcaggagat
     6181 ctcccgttcg ttacgtcagc aaggggaact ggtgggacag cgtttgcaac tgcgccagca
     6241 acaacagcaa ctcannnnnn nnnncgcctg gcgcaaggtc aggcgaataa tgcggcaacc
     6301 tccgccggag cgacccaggc cgggatttac gatttgatcg aacaacatca gcgtcaggcc
     6361 gcagaaagtg cactcgatcg gctgattgat atcgatcttg agtatgttaa ccagatgaat
     6421 gaactgcgcc ttagcgccct gcgggtgcag caaatggtga tgaatctggg gctggaacag
     6481 atccagaaaa atgcgccaac gctggaaaag cagctcaata atgcggtgaa aattctgcaa
     6541 cgtcggcaaa tacgcattga agatccgggt gttcgtactc aggtagcaac aacgttaact
     6601 accgttagcc aatatagcga tttgctggcg ctgtatcagc aggacagtga aatcagcaat
     6661 cacctgcaaa ctctcgccca aaataacatc gcccaattcg cgcagtttag tagcgaagtc
     6721 agtcagctgg tcgacaccat tgagctgcgt aatcagcacg ggctggcaca tctggaaaaa
     6781 gccagtgcac gcgggcaata cagtctgtta ttgctgggat tagtttctct ttgcgcactg
     6841 attctgatcc tctggcgcgt ggtttatcgc tcagtcacgc gccctcttgc agaacaaacg
     6901 caggcgctgc aacgcttgct ggatggtgat atcgactccc ctttcccgga aaccgctggc
     6961 gtacgggaac tggataccat cgggcggctg atggatgcgt ttcgcagcag tgttcatgca
     7021 ctgaatcgcc accgcgaaca gctggcggcg cagatcaaag cgcgtacagc tgaattgcag
     7081 gaactggtga tagaacaccg acaggcacgg gcggaagcag aaaaagccag ccaggcaaaa
     7141 tcggcgtttc tggcggcgat gagccatgag atccgcacac cgctgtacgg tattctcggc
     7201 accgcgcaac tgctggcaga taaccccgca cttaacgccc agcgcgatga tttgcgggcg
     7261 attaccgact ctggtgaatc gttgctgacc atcctcaacg atattctcga ttattcggct
     7321 atcgaagcag gtggaaagaa tgtttcggtc agcgatgaac cctttgaacc gcgcccgctg
     7381 ctggaaagta ccctgcaatt aatgagcgga agggtgaaag gtcgcccgat tcgcctggca
     7441 acagaaattg ccgacgatgt accgactgcg ttaatgggcg atccgcggcg tattcgtcag
     7501 gtcattacca acctgttgag caacgccctg cgtttcaccg acgaagggca cattattctg
     7561 cgtagtcgca ctgatggcga acaatggctg gtcgaagtgg aagacagcgg ctgcggtatt
     7621 gatcccgcga aactggctga aatcttccag ccatttgttc aggttagcgg caaacgcggc
     7681 ggcaccgggc tggggctgac tatcagtagc cgtctggccc aggcgatggg tggcgaactg
     7741 agcgccacca gcacgccgga ggttggaagc tgtttttgtt tacgcttgcc attacgtatt
     7801 gccactgcac ccgtgccaaa aacagtcaat caggcggtgc gtctggacgg tttacgtttg
     7861 ctgttaattg aagataaccc gctaacccag cggattaccg ttgagatgtt gaacagcagt
     7921 ggggagcagg tcgttgccgt aggaaatgcc gcgcaggctt tagagacact gcaaaatagc
     7981 gaaccgtttg ctgccgcact ggtggatttt gatctgccgg atatcgacgg cattaccctt
     8041 gcccggcaac tggcacagca atatccgtca ctggttttga ttggctttag cgcccatgtc
     8101 attgacgaaa cactgcgcca gcgtaccagt tcgctatttc gcgggattat ccctaaaccg
     8161 gtgccgcgtg aagtgctcgg tcaattactg gcgcactatc tccaactgca agtcaataac
     8221 gatctaccac tggatgtatc gcaactcaat gaagatgctc atttaatggg ggcggagaag
     8281 atccacgaat ggctgatatt atttaaacaa catgccctgc cgcttctcga tgaaatcgac
     8341 attgcccgcg ccagtcagga taacgagaaa ataaagcgtg ccgcacatca gctaaaaagc
     8401 agttgctcga gtctgggaat gcgtagcgcc agccagcaat gcgcacaact ggagcagcag
     8461 ccattatctg ccccccttcc acacgaagaa attacacgca gtgttgccgc tctggaagca
     8521 tggttaataa gaaagacctg aacgcgattt gaatcaatat cagccagtta agaacaacta
     8581 gaatagtggc atttggtatg caacttaacg gactggcaaa atcatggcag agaaaaaaag
     8641 aacccgctgg cagcggcggc caggcacgac gggcggcaaa ttaccgtgga atgactggcg
     8701 caatgccacg acctggcgta aagcgacgca attattactg ttggcaatta atatttatat
     8761 tgccatcacg ttctggtatt gggtgcgcta ttacgaaacg gcaggtagta cgacatttgt
     8821 caccagaccg ggaggtatcg aaggctggct accgattgcc ggtctgatga atctgaaata
     8881 tagccttgca acaggtcagt taccgtccgt ccacgccgcc gcgatgctgt tattggtcgc
     8941 ttttatcgtt atcagtctat tactcaaaaa ggccttttgt tcatggttat gcccggttgg
     9001 tacgctttcg gaattaatcg acgatctcgg taacaaactg tttggttggc aatttgtcct
     9061 tccccgctgg ctggatattc ctctgcgcgg cgtgaaatat ttgctgttga gtttttttct
     9121 ctatatcgcg ttattgatgc ccgctcaggc gattcactat tttatgttgt cgccctacag
     9181 cgtggtgatg gacgttaaaa tgctcgattt ctttcgtcat atggggaccg cgacattaat
     9241 cagcatgatc gttttgctga ttgccagcct gtttattcgc catgcctggt gtcgttatct
     9301 ttgcccatat ggcgcgctga tgagcgtggt ttcgctatta tcgccgttta agattcgtcg
     9361 caatgccgaa agttgtatcg attgtggcaa atgcgcaaaa agttgcccat cacggatccc
     9421 ggtcgataaa ttaattcagg tacgaacagt ggaatgtacc ggctgtatga cttgcgtaga
     9481 gtcatgtccg gtagcctcaa cattgacctt ttcactgcaa aaacctgcgg caaataaaaa
     9541 agcctttgcg ttgtctggct ggttaatgac gctactggtt atggggatta tgtttgcggc
     9601 gattggttac gcaatctatg cgggagtatg gcaaagcccg gtgccggagg aattgtacca
     9661 acgcttaatt ccgcaatcgc caatgattgg tcactaaatt agcaacaagc ggaacatcac
     9721 ctcgcacggg gtgatgttca caaaatcaca ttattttgat tttgacatca taactttcaa
     9781 gcccggtcat tttttctcgt gcggtgaagc gaatttcaga gacttctttc cctgtattct
     9841 tgttcagctc ggcgattttt tgcgcgataa agtcggcgat ttccgtttcg gcttgttttt
     9901 ttaactcttc aatattcact gttaacctct tctggtctta aaagacagca acacgccagt
     9961 aaggtgttgg ttcccggtac cctggtcagg taagaaatgt tataagccat ctccccgaaa
    10021 gattcaataa tcaacaggtt atcgcttact atacgtacag cattgaagaa aaataaggca
    10081 gatataaaaa aggctccacg agggagcctg atattatttt gtgaaatcca gcggcgcatg
    10141 gtcagtgcgt atttgcagcg caacgttatc aaaggtgatc atcgtcgatt tacaaaaaat
    10201 gcactttgca cccaaagggt ttcgctccgt cacatcgaat gccgatgtgc ggtactgtga
    10261 accatggcaa catggacagc ggaaatggag atgttgagta ataacggcaa ccttagagcg
    10321 taacaacgtt cgctgccgcg ggaccacgtt gcccctgctc aatagaaaat tcaactttct
    10381 gattttcgtt cagcgtgcgg aattcattgc tctggatggc agtgaaatgg acgaaaacgt
    10441 ctttgctgcc atcatcagga gtgataaagc caannnnnnn nnncgttaaa ccattttact
    10501 aaaccagtca ttttattaga catattttac ttccttcatt ttgggccttt cggcaaacag
    10561 ggctttaata cagaaactac ttagtgttca ggagagagac tcaaaagaag gggataatgc
    10621 ctgataatga gaactgcttt agtaaactac tttgtatgct gtctgtcttt caaaccgacg
    10681 cagctattaa cgcatgataa ttgagattaa gcaaatttta ttttagccgt ccggcggtct
    10741 taaatatcgc tggctttatt ttattaaccc cgtatagtgc agcgcagtat tttcagtaag
    10801 gaatttgttt gtcccgtaaa atgacaggaa ttgtcaaaac ctttgatcgc aaaagcggca
    10861 aaggattcat tatcccctcc gacggtcgta aagaagtcca ggtccatatt tccgcattca
    10921 ctccccgcga cgcagaagtg cttataccag gattacgcgt ggaattttgc cgcgtaaatg
    10981 gcctgcgtgg accaacagcg gcaaatgttt atttgtcttg atggagagcg cacgctggta
    11041 tgcgtgcttc attcacgatt tgcgtaaaac ctgccaacga acacatgccc tgcgcatttc
    11101 gctcttcaca tcctgccagg gtcagtttca cctctccggg aggcgtattt aatgacagcg
    11161 gcgttttatc acgcatctgc tgtaaagtct ggaagaccaa cgaaacctga atccactggc
    11221 tgttatcgct gagccggcgc cagcgttcaa acaccagttc accacctggc ggcgtgttat
    11281 ccggctgacc tggaagcgtc cagttgagct ccagtgcgcc gccgagattt gccagattag
    11341 tatcgtgtcc ggcaataaac agcactgaag tgggtaatgt cacaccatac gcctgttttt
    11401 gcggtggatg gggcgtcaac gctgccatga tcaaatccaa taacggggtg gcgcgactgc
    11461 gggcaacctc tggcgtgcgt tgtagtaaat aaaattgcgc gttatgcaaa cttagcaagg
    11521 tgttccactg gtgtgaatca gtgatccttc cccaccccgg ctccggcatt ccctgtgctt
    11581 gttgcaggag aaatatctcc gtcagcattg atgcgaggct taccgcaccg gttaatgaga
    11641 cattgtcggc gctcaccttg agttccgatg gtaatgcctg cgttaatgaa cagctttcgt
    11701 cctgtttctt acggttaagg cacaagtttg attgcgggaa attaagcacc cgttccagtt
    11761 cgcgaaacgc cgtttgccga tgcccggcaa agtcagcaat tgcccctcct gccctactga
    11821 ggatcgcgtc agtcacgttc gcgttatcca gttggcaaac gccagttttt agaggattaa
    11881 ataacggatc gggactggac gtatctgcct gggtatgtac ggttattgca cagtcaggtg
    11941 ccagcccggc ggcgaaggct tcgcctgttt tacgggtacg ttcgtcgaca tcagcaataa
    12001 ccgcgacctg acctggctgc gggcagccct tttttgccag caatccgtcg gccaccagac
    12061 gctggcgttg gtaatgtccg agataagcga ttaactcacc accgcgcggt gtcagccaac
    12121 ccagttttac cggccaggtt ggccatgcgt caggggtgac atcctgcatc agttgcgtgg
    12181 ccttggttgg tgcacgcaca ccgtgacgac tgacaatcac cacactttcc agcttcagct
    12241 ccggctcact ctgagcgaat gcagatttcg gtgttaacgg aatcagaaga gataagaatg
    12301 ggatcaagat cgctttcatc gatatgtttc cgcttttcat atccgacatt attgattgcc
    12361 tgatgcgacg ctaatgcgcc tcatcaggct taccttcgtt aacttttcaa tcgcgggtcc
    12421 agccatacca acacaagggt ggagagcgaa cacatcaaca aaatgccgac gaaccaaagt
    12481 aaataccaca ttgttcctgc tccttagtac agctcgtttt cgttacggcg gagagtttct
    12541 gtcgtcatgc gcccccacat tttgtagtag ctccagagag tgtagagcaa cacaatgggc
    12601 aaaaatatca gcacgattac caacataatg ctcagcgtca gctgactgga agtgctgtcc
    12661 cacaacgtca ggctggagat cggactcacg cttgacggca tgacaaaggg gaacagcgtg
    12721 annnnnnnnn natgacgcca tcaaaaatcc ccaacccggg cgaccacgat aaatcgccat
    12781 caccgtcagc aatgggcaga agaaccccag cagcgggaag atccacaaaa cgggcgattc
    12841 gacgaaatta ttcatccagg cactaggtag cactgccacc agtttcatta acggattgga
    12901 aggaccgtta gcatcctggg cgagcagtac aaagccatca ataccaaccc acagccagta
    12961 accnnnnnnn nnntgcggcg cgtttggtcg ccagctgtga acgcagatga atcacaccaa
    13021 cagttttcag ttgtaaccag acgccacctt gcaaaatcac catcccaaga ctgagcaatc
    13081 cacacaataa agggaatggc gtcagcagtt gccagaagct gccgagatat tccacgcgta
    13141 attgcggtgt gaaagcaaac ggcacgccga gcagcaagtt gccgaatgca ataccgaaga
    13201 ctaccggcgg caccagactg ccgatgacca gtccggcatc ccacatttta cgccagcgtg
    13261 catcggcgat ttttccgcga taatcaaagg ccagcgggcg gaagaacagc gagcacagca
    13321 ccagaatcat cgccacataa aagccggaaa acgccgctgc atacactctg ggccaggcgg
    13381 caaataatgc cccacctgcg aggatcaacc agacctgatt gccttcccag tgcgcgccaa
    13441 cgctgtttat cactatccga cgttcatcat cattacgcgc caccagcggc agcagacagc
    13501 cgatccccat gtcaaatccg tcggagatca taaagaccac caggatcacg ccaatcagca
    13561 gccaccagat gaagcgcaat gtttcataat caaacatgat tttctccttt acccctgttg
    13621 ctgcgtcggt tgttcactct gcatcgcgct cggccccaga cgggcatatt tctgcatcag
    13681 gtagacttcg gcgattaaga acagggtgta aagccctacg atcatgatca gtgagaaagc
    13741 cagttgtcct gtggttaagg cggagtgcgc ggagtatgtc ggtaagatgt cctgtatcgc
    13801 ccacggctga cgaccaaact cggtcataaa ccatccggct tcaatcgcaa tccacggcag
    13861 tggcaaactc cagagcgcca ttttcagcac ccagcgatgc tggtcgattt tgccacgcag
    13921 cgtctggaca agcgcaatca gcatcaccag taacagcagg gaaccacagc ccaccatgat
    13981 gcggaaactc cagaataccg gcgcaacctg aggtatcgcg ccacgcatcg ccgcctggta
    14041 ctgtgcggct gtgacatgat tcatatccgg cgcatagcgg gagagcaaca tgccgtaacc
    14101 caggtcgcct tccagctccc ggaatgcctg caacacatgc ggctcacgat tgccttgcga
    14161 tatttcctgc attaacagcc acgccatacg tccgcgttgc aagcgtgggt aggtttcagc
    14221 catcaaattc ttcagacccg gcacgggttt atctaatgaa tgagtggcga ggattcctag
    14281 cagcgcggga attttgatgg caaaggcgtt acgctcttga tcctgttccg gccaggcaac
    14341 cacatggaac ggtgcaggtg caggttccgt ttgccactcc ccttccatcg ccgccagttt
    14401 taccggttgt acttgcgcga cttcatacgc agaactgtct ccgagttgca gcgtaccgat
    14461 aatcgccaga gtaccgaaga cggaaccgat ggcaaacgag cgtaatgcga catcgcgctc
    14521 ccgtccgcgc agtaaatacc aggcgctgat cgccataata aacatggccc cggtcacgta
    14581 tcccgccatt acggtgtgca caaatttcac ctggctgacc gggttaaaga ccagctcgct
    14641 gaaactggtc atctccatac gcagggtgtc gatatcaaaa tgcgcaccag tcgggtattg
    14701 catccaaccg ttggcattca atatccacaa cgcagagaga tttgaaccga acgccaccag
    14761 ccacgtcacc agcaggtgct ggtatttatt cagccgctgc cagccgaaga agaacagccc
    14821 gacaaaggtg gattcgagga agaaggccat taatgcttcc atcgccagcg gtgcgccaaa
    14881 aatatcgccc acatagttgg aatagaatga ccagttagta ccaaactgaa actccatggt
    14941 caggccggta gccacgccaa gagcaaagtt gataccgaag agcttacccc agaagcgcgt
    15001 catatcgcgg tagattgttt tgccggtgac cacgtaaatg gtttccatga tagccagcaa
    15061 aaaaatcagc cccagggtaa ggggtacaaa taaaaagtga tacagcgcgg tcagagcaaa
    15121 ctgccagcgc gataaatcaa tgacatccca catgcgcact cctgtaggcg aagagagaaa
    15181 aataacccgt taatggtaga tctccctctt tatcctgaaa ctctacgacc agaggtattc
    15241 gccgattgcg atattcataa gcctgaaagc gatacttacc gtctttttac gtcggtgccc
    15301 cctccgccag ccactggcat acctcgctaa gccgctgcgc agagtcgacc aaatcttctg
    15361 gcgctgccag caccacttcc ggtattgggc agatttcata actttccagt aacgggcctt
    15421 ttaaggtgtc cgtacagcgt aaatgccaga catggcgtaa ccccgtggcg ttgatatagc
    15481 tctcgccata gccaatggta cgaatctgaa tatttcccgg cccacagaga cgtgagagaa
    15541 aaagccggtc agcttcgctg atgggtaatt gcgtcatatt aatgctgtgc ggctgcgcgt
    15601 cggggttacg tacatgtgcc agtaattcat gggccaacgg taggccattc atcaggccat
    15661 caatgggcgg cggtaacagc gaatctgtcg gcaagacgtt ttgcgtcgcc gcctgccaca
    15721 acgccagcgg tgcgcagcca gcctccagtt tgtcttccag caactgttcg ccgtgacgtc
    15781 tgcgtacccg ccataatccg cagaagatcg cctcctgaat ttcactttca ctgtcgtcag
    15841 cctgctgaat gcgcactgac acttcccctt ccccgagaag cgtattgata aagtgacgat
    15901 cgtcggcgtt gagatttaac agatcgactg ccagtacctc acccagcggc ggctggcgtt
    15961 cgcttagttg atgttgtaac tcgtttaaca gcgcaatcac ctgcgggctg tgagcacatt
    16021 gctccagggc cgccatactc ggttcaccat tcacccgaca ggtgatcggc agtggattca
    16081 tgctgaaact gtcatcgttc ggttgcgttc ctggccccag cagatggaaa aaagtttcgc
    16141 tcatgaagca cgctcctgtt gcggttcgac aagcccgcgc atcaggttta tcagttccgc
    16201 ccacgggtga ataccattca gcacgccgcg atagtttccg ccggtaaaca ctaatgtggc
    16261 gggaaagcga aagacgccaa agcgatcgcc gatggcttcg ctctgctcaa gatcagcaat
    16321 cgctacctgc catgtatagt cgggaaactc gcgcagtaat tcgccaatca ttaccgggtt
    16381 atcgctgact tctggcgtgc gtttcgggtc actgcttaat aacaccacgc cgtctggcgc
    16441 ttgcgtaagc cagtcgtcga gacgggattc actgaccggc gtccagccac gcgccagcat
    16501 tcgttgccac aacgcatcaa atggcgtgtc cttgctcatc cctgctcctc cagtgtcatc
    16561 cggtactgac gcaagcgaac gccttcgtaa ttttccatcg acagacagtc ataattgaga
    16621 cagcgcgatt cattagccgg ttgcggcaca attccccagg ccgccagctg taccagcgcc
    16681 gcctgttccg cggcgggcag ttgctcccgc gccagttcgc tcaggctgcc gccgtagtcg
    16741 tcgagcatcg cgggttgcag accgacgagg gcaatatgtg ctggcagatg tccgcggata
    16801 tctgccagcg ccagcacttc ggagaaactg ttctggtgca ggctcatttt cttcgcgctg
    16861 agataagccg gaatgcgttc tccggcatac gttcgtagcg ttccgggttc cagcccgtaa
    16921 tcaatggcat cgagaatcaa cagatgactg gcgctttcga cataccccag caagttcagt
    16981 ccttgggtgc cgccatcgac aatctccaca tcctcgggcc agtggtaatg ggcatacagc
    17041 cgttccgcca cccgcacgcc gaagccttca tcggcccaca gcaggttgcc cagccccatt
    17101 accaccacgc gttgctcgct catgaacgct ccttgttact tattttgcca aatttgtggc
    17161 tacggtagcc gttgaccata gtggagatca ccgtgtcgtc ggacatgatg tcttcacgca
    17221 gcgccatgta gacatgaccg atcacaaacg cgccaatcag ccacatcccc agccgatgcc
    17281 agctgtgaat gtccattgag ttgccacccg tccagtagaa aaattccacc acataacgga
    17341 acggcgcaaa aatagcgtac tggctgtgtt cgctgtacag cgcaaagccg gtgatgatca
    17401 taaaaactga catcaggaaa tagccgaaca tcgccgcctg ggcgatgggg ttatggccta
    17461 tatcggcact cggacgtttt gccagaaaca gataccagcg gatttcatac cacacgccct
    17521 gccaccagct tttacgccat accggtacga taaacagctc gcgggagtaa cgattgccaa
    17581 caaaagccca gtagatccgc atcagcaaga ccacggtaaa aatcatcccg gcgctgaagt
    17641 gaattaacct gatgtagccc atatagaaca gatacgtcgc ctcgccgctg acggaaggta
    17701 gcggcttgcc gataaagtaa ccggtgacca tcaacaccgc catgcataac actgtcagcc
    17761 agtgccatat ccgcacgggt gcttcaaaga catagtgact gacaacgttg tcgcttttct
    17821 gttgcatgat gattctcctt cgctgttaac gcacctgcac ggagataagc tcgctaccgt
    17881 cgtcgcccag cacgtgtgtt gaacaggcga ggcacgggtc aaagctgtgc agagtacgca
    17941 ggatctccag cggttgctcg gggatcgcca ttttggtgtt catcagcgcc gcttcataag
    18001 cgccaatctg ccctttggga tcgcgcgggc tggcgttcca ggtggtcggc accacgcact
    18061 ggtagagatc aatcttgcca tcgcgaatgg cagcccagtg gcctaacgcc ccgcgcggcg
    18121 cttcggtaaa accgacacca cggcactctg tcggccag
//
LOCUS       DAWDRC010000015        17795 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1723_length_17795_cov_2.673055, whole genome shotgun sequence.
ACCESSION   DAWDRC010000015 DAWDRC010000000
VERSION     DAWDRC010000015.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17795)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17795)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1723_length_17795_cov_2.673055"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12294..12303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14370..14379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14795..14804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4164 a         4491 c         4818 g         4292 t
ORIGIN      
        1 cgtgataatg atgacaaggg gcaaggtcga tttcgcctgc gtttatttgg ttttcagcgt
       61 cttgctggct ggtggcccaa ccttcgtaca gcatggcacc aataatcgcc ccttttaccg
      121 gaccacacat ctcttcccag gcaatcggcg ggccagagtg caaaatacgt ttttgttcag
      181 ccattgctgg aatggcctct ttagcgagca tgacatccac cagcacgggc tgtgcttcga
      241 gataacgtgt catggcgatt tggttggcat tttctaccag gggatgacgt agcaacgatg
      301 ccagatccaa tccagcgtca atatcgcctt gtgctggtgg ctgccagttg agtgcaattg
      361 cgcttccccc ggcatgttga atattgtcag caaaactgtg tagtcccgcg ttcacaacct
      421 tcaaaggctg attaaataag gtattcatgc gtttagcccc tttttctgca actgagtctg
      481 tgcaacggtg ctggcccata gcgccgcctg tgcattgctg ctggcgatga tgacccctgc
      541 attttgtagt gcgcttatct gctgactgcg tatttgcgga tcagcttccg ttccgcaaac
      601 gtgcgcaatc agcaacggtg cgttattcat gtcgatgtgt gaaagttggt ccagtaattc
      661 gctggcaggc gccgtggaag cgccataacc aagtaccagg tcgaataaca cgacggcagt
      721 gtgtgagtcg ttgagttcgt tgagcagacg ttgattgcgc agagtcgggt cgatcattgg
      781 atgcggtttg ccacgggtga aatcatcgtc gcccatatcg atcaacgtgt gatcttcgct
      841 ttgccagata tttgccagag cacgattacc cgcaactggc gtattggaag ctgcgctgaa
      901 tcccttttgt tggcagataa gttgcgcttc gtagcagaag gttcctccgg cgaaaacacc
      961 gcgtatcgcc tggcgatggg ctggcaggcg ctgacaggcg ttttgtaaca ttgtgagatc
     1021 ggcgcaggaa atctcggtct cgacagccgg aagtggttga tcattcagta acgccacggc
     1081 gatatttgcg gcgctggcaa gtgtggtggc cgcggtgata ttgggacgtg ccagatcatg
     1141 cggattcgca ccgaggaaat tcaccaccac gggttttcca caagcctcgg cgcgctccag
     1201 aatagtccgg gcaacgatcg gtgaaggcgg ttttgaaatc agcacaatta cgcgagtttc
     1261 atcgtcttgc gccagcgcat ctaaagcgaa gagcatcgat attccaccaa tctcttcact
     1321 gagatcatgt ccgcctgttc ccaacgcctg agaaataccc gctccgagct ggtcgattcg
     1381 gcaagtcact tcctgtagcc cggtgcctga cgcgccaata acaccaattg ctccgcgttt
     1441 caccacatta gcgaagccta acggaatgcc gttaacaatg gcggttccac agtcaggtcc
     1501 cattacgatg cgctgacgtt ctcgggcaag cgttttgatg gatttttcct gcccgatact
     1561 gacgttatcg ctgaacatca tcacgttcat tccgaggttt aacgctttaa ttgcttcggc
     1621 ggcggcgtag tctcccggta cagaaatgag tgccaggttg gcttcgggtt cggcttccag
     1681 tgccatttcc aggctgacct tttccggtga tttaatgcca ctatccgttt catcatcggg
     1741 tttgctggtc agacgttgct gggccagtgc cagtgcttca ttgcaaatat cttcttcacc
     1801 catgactgca atcactaaat cattgggact ggcgttgatt tcgttaccca gacccgcatc
     1861 gcgcaattgt tcgagattat tgggcgttcc catgacgacc gaggcctgtt gaataccggg
     1921 caacttgctg atttgcgcgg aaatctgcat cagagaaact gagtcctgat aaagattact
     1981 gaaaacttta tatttaactt tcatgctgtt acgcctgttt cattccgccc gatagcaaaa
     2041 ttcgggcaaa gcatccccgt gtagccccat aaacgggcta cgggagaaaa ggttgaaatc
     2101 aattcatggt gttcagggtt cgaaagccat gcagcattcc tgcaaggcaa tctactcctg
     2161 acgttgcgcc aaattgcatg acctgttctg cacaggatgc gattgtcatt gccgatgcgc
     2221 tcccaaccag ttgtgcgagt aactggcaaa tcggttctga aaaatgtccc tgaagcgcac
     2281 gctccagata atgacgactg atatccgtgg tgttgtgcgc atgttgatca attgcctgct
     2341 gcaaccggta ttgatgatcg gcaagtggtg cagggagttg ccataaccag agagctgcca
     2401 gatagcccaa caaatagtca tctccatcgg gcgttaagcc tttaccaaag ccgatcagtt
     2461 gtggcacctg gcgttccagc gcatctggat gttccataat cgctaatccc tggtgcgtga
     2521 ggctatccgg caggtgcaat gtgctttgaa cgttatgttc cagacaaaat aaaagccgtt
     2581 gcgatgccat cgttgggtag tgctggaaaa ccaaagggaa tgcctcaagt gtcagcattc
     2641 cccccaaaaa tggcgactgc cagagcgttg cgttatcgag cgccacacaa aatcgaggtg
     2701 tccgcagtaa gcctggggtt aactgaatgg gatctgaatg ggcaatctct cttcgccagt
     2761 cccaacattc aggtagccag actctggcag catcagggag attcagatac gtttgcgcac
     2821 acaacagagt gatgaatgtt cccgcgtctg aatacagatt taccgcatgt ttaaaagcgc
     2881 tatgtagacg taaatcttgc gtttcattct gcgggaaaag atatcccaac gacgatgcgc
     2941 aaagcgtaac ctgtaccatc ggcgttagcc ttccagacct gcaataatgg ctttggaatc
     3001 agtaacccaa ccgaaaatgg caccttgtgc tttgatcatc tcaagggcgt atttctggaa
     3061 ttccgggaaa taggagccga cacaatcttc cgggataatg cattcatagc cacgatcatt
     3121 tgcttcgcgc acggtggtgg ttacgcaaac ttcggttgtt acaccacaaa ctatcagcgt
     3181 cttgataccg tggttttgca aaatcagatg taagtcggtt tgataaaacg cgcctttgcc
     3241 aggcttatca atcaccggtt cgcctgccac cgggtacagt tcaggaatga tgtcatgacc
     3301 tgcttcacct ctgaccagaa tgcgtcccat cggtcctggt tcaccaataa aggtttttcc
     3361 gccgcgcgtc agtttggctg gcgggcagtc gcttaagtct gcacgatggc cttcacgagt
     3421 atgaataacc ataattcctt tttgacgtgc ggctgccagc acctctgtac atggtgcgat
     3481 agccgtgcgt accagtgaaa catcattgcc cagagcctca ccgaagcccc ctgcttcgac
     3541 aaagtcacgc tgcatgtcga tcatgaccag tgcagttgtg cgcggatcaa aaggtaattc
     3601 aaagggttga gcctgaaaaa tggattgcgt catggtaaat ctcctgtctg atgtttaaac
     3661 gatccagttg ttctgagaac gagtattcag gagaaaagcg acacaaaaca ggaaaaaagt
     3721 ttgccgcaca gcaacgaaat ttttgcattc agcaggtggt gtgatgacga atgtgaaggg
     3781 agtcaaagag tggcatacag caacaaattt tttgatgctg tatggttttg agattaaaaa
     3841 aatcgttgaa attgtgtgag cttaatcatc agatttagcg actatggcgt tactttttaa
     3901 caccatactg tgtaaaccat tccaacattc gctgccagcc gtctttcgca gactcggcat
     3961 gatagctcgg acgataatcg gcgttgaacg catgccctgc atccgggtac acgataatct
     4021 ctgctttcgc gtttgctgcc cgcagcgcct ggcgcatcgt ttccacgctc tcttgcggaa
     4081 tgctgttatc ctggccgcca tataagccga gaaccggagc gttaagatcg gttgcgatat
     4141 caacaggttg tttcggtgaa ttcagcgact tatcgcccgt cagtttgccg taccacgcca
     4201 ctgcggcttt tagctgtgga ttatgcgcgg catacagcca ggtgatacgt ccaccccagc
     4261 agaatccggt gatcattaaa cgatgaacat cgccgccgtt gcgggaagcc caactggcga
     4321 catgatcgag atcggccagc acctgcgagt caggcacttt tgctaccaga ccgctaagca
     4381 acgtggggat atcggcaaaa tcattcggat cgccttcgcg gaagtaaagt tcaggtgcga
     4441 tagccagata cccctccagc gccagacggc gacaaatgtc gcggatatgt tcatgcacgc
     4501 caaaaatttc ctgcactaca atgaccactg gcagtgggcc atcgctttgc tttggtctgg
     4561 catggtaagc aggcatgtta tccccttgtg aagggataga ggtgaagccc gccacaatcg
     4621 cgtcgtccgg agtctgaacg atggtcgaag cgagaggcga tgcagcaggt gcaaatccag
     4681 attgttgtgt tgttgccatg gtattctccg tacccttata aaaatgttgc gcaatgttaa
     4741 ctatagtcag catgcaacaa atcacattgc ctgaatcggc tcatctttta tgcagtcctg
     4801 cagaatgaag agtgatttat gtgatttgca tcacttttgg cgggtaaatt tatgcaacgc
     4861 attttcgtca tggtgatgag tatcacgaaa aaatgttaaa cccttcggta aagtgtcttt
     4921 ttgcttcttc tgactaaacc gattcacaga ggagttgtat atgtccaagt ctgatgtttt
     4981 tcatctcggc ctcactaaaa acgatttaca aggggctacg cttgccatcg tccctggcga
     5041 cccggatcgt gtggaaaaga tcgccgcgct gatggataag ccggttaagc tggcatctca
     5101 ccgcgaattc accacctggc gtgcagagct ggatggtaaa cctgttatcg tctgctctac
     5161 cggtatcggc ggcccgtcta cctctattgc tgttgaagag ctggcacagc tgggcattcg
     5221 caccttcctg cgtatcggta caacgggcgc tattcagccg catattaatg tgggtgatgt
     5281 cctggttacc acggcgtctg ttcgtctgga tggcgcgagc ctgcacttcg caccgctgga
     5341 attcccggct gtcgctgatt tcgaatgtac gactgcgctg gttgaagctg cgaaatccat
     5401 tggcgcgacg actcacgttg gcgtgacagc ttcttctgat accttctacc caggtcagga
     5461 acgttacgat acttactctg gtcgcgtagt tcgtcacttt aaaggttcta tggaagagtg
     5521 gcaggcgatg ggcgtaatga actatgaaat ggaatctgca accctgctga ccatgtgtgc
     5581 aagtcagggc ctgcgtgccg gtatggtagc gggggttatc gttaaccgca cccagcaaga
     5641 gatcccgaat gctgagacga tgaaacaaac cgaaagccat gcggtgaaaa tcgtggtaga
     5701 agcggcgcgt cgtctgctgt aattatcttc tctggtctga aggccgacgc gttcggcctt
     5761 ttgtattttt gcgtagcgcc tcgcaggaaa tgcctttcca actggacgtt tgtacagcac
     5821 aattctattt tgtgcgggta agttgttgcg tcaggaggcg ttgtggattt ctcaatcatg
     5881 gtttacgcag ttattgcatt ggtgggtgtg gcaattggct ggctgtttgc cagttatcaa
     5941 catgcgcagc aaaaagccga gcaattagct gaacgtgaag agatggtcgc ggagttaagc
     6001 gcggcaaaac aacaaattac tcaaagcgag cactggcgcg ctgagtgcga gttactcaat
     6061 aacgaagtgc gcagcctgca aagtattaac acctctctgg aggccgatct gcgtgaagtg
     6121 accacgcgga tggaagctgc acagcaacat gctgacgata aaattcgcca gatgattaac
     6181 agcgagcagc gcctcagtga gcagtttgaa aacctcgcca accgtatttt tgagcacagc
     6241 aatcgccggg ttgatgagca aaaccgtcag agtctgaaca gcctgttgtc gccgctacgt
     6301 gaacaactgg acggtttccg ccgtcaggtt caggacagct tcggtaaaga agcacaggaa
     6361 cgtcataccc tgacccacga aattcgcaat ctccagcagc tcaacgcgca aatggctcag
     6421 gaggcgatca acctgacgcg cgcgctgaaa ggcgacaata aaacccaggg gaactggggg
     6481 gaggtggtat tgacgcgggt gctggaggct tccggtctgc gtgaagggta tgaatatgaa
     6541 actcaggtca gcatcgaaaa tgacgcccgc tcgcggatgc agccggatgt catcgtgcgg
     6601 ttgccgcagg gaaaagatgt ggtgatcgac gccaaaatga cgctggtcgc ctatgaacgc
     6661 tatttcaacg ccgaagatga ctacacccgc gaaagcgcgc tacaggaaca tatcgcgtcg
     6721 gtgcgtaacc atatccgttt gctgggacgc aaagattatc aacagctgcc ggggctaaga
     6781 accctggatt acgtgctgat gtttatcccc gttgagccag catttttatt ggcactggat
     6841 cgccagccgg agctgatcac cgaagcgttg aaaaacaata tcatgctggt tagcccgact
     6901 acgctgctgg tggcgctgcg cactatcgcc aacctgtggc gttatgagca tcaaagtcgt
     6961 aacgcccaga aaatcgccga tcgcgccagc aagctgtacg acaaaatgcg cttgtttgtc
     7021 gatgacatgt ccgcgattgg tcaaagtctc gacaaagcgc aggataacta tcgccaggca
     7081 atgaaaaaac tctcttcagg tcgcggaaat gtgctggcgc aggcagaagc gtttcgcggt
     7141 ttaggggtag aaattaaacg cgagattaat ccggatttgg ctgaacaggc ggtgagccag
     7201 gatgaagagt atcgacttcg gtcggttccg gagcagccga atgatgaagc ttatcaacgc
     7261 gatgatgaat ataatcagca gtcgcgctag cccattgggg gtagttaagc cgggtagaaa
     7321 tctagggcat cgacgcccaa tctgttacac ttctggaaca attttttgat gagcaggcat
     7381 tgagatggtg gataagtcac aagaaacgac gcactttggt tttcagaccg tcgcgaagga
     7441 acaaaaagcg gatatggtcg cccacgtttt ccattccgtg gcatcaaaat acgatgtcat
     7501 gaatgatttg atgtcattcg gtattcatcg tttgtggaag cgattcacga ttgattgcag
     7561 cggcgtacgc cgtgggcaga ccgtgctgga tctggctggt ggcaccggcg acctgacagc
     7621 gaaattctct cgcctggtcg gagaaactgg caaagtggtc cttgctgata tcaatgaatc
     7681 catgctcaaa atgggccgcg agaagctgcg taatatcggt gtgattggca acgttgagta
     7741 tgttcaggcg aacgctgagg cgctgccgtt cccggataac acctttgatt gcatcaccat
     7801 ttcgtttggt ctgcgtaacg tcaccgacaa agataaagca ctgcgttcaa tgtatcgcgt
     7861 gctgaaaccc ggcggccgcc tgctggtgct tgagttctcg aagccaatta tcgagccgct
     7921 gagcaaagcc tatgatgcat actccttcca tgtgctgccg cgtattggct cactggtcgc
     7981 gaacgacgcc gacagctacc gttatctggc agaatccatc cgtatgcatc ccgatcagga
     8041 taccctgaaa accatgatgc aggatgccgg attcgaaagt gtcgactact acaatctgac
     8101 ggcaggggtt gtggcgctgc atcgtggtta taagttctga caggagaccg gaaatgcctt
     8161 ttaaaccttt agtgacggca ggaattgaaa gtctgctcaa caccttcctg tatcgctcac
     8221 ccgcgctgaa aacggcccgc tcgcgtctgt tgggcaaagt gctgcgcgtg gaggtaaaag
     8281 gcttttcgac gtcattgatt ctggtgttca gcgaacgcca ggtcgatgta ctgggcgaat
     8341 gggcaggcga tgctgactgc accgttatcg cttacgccag cgtgttgccg aaacttcgcg
     8401 atcgccagca gcttgccgca ctgattcgca gtggtgagct ggaagtgcag ggcgatattc
     8461 aggtggtgca aaacttcgtt gcgctggcag atctggctga gttcgaccct gcggaactgc
     8521 tggcccctta taccggtgat atcgccgctg aaggtatcag caaagccctg cgcgggggcg
     8581 caaagttcct gcatcacggc attaagcgcc agcaacgtta tgtggcggaa gccattactg
     8641 aagagtggcg tatggcaccc ggtccgcttg aagtggcctg gtttgcggaa gagacggctg
     8701 ccgtcgagcg tgctgttgat gccctgacca aacggctgga aaaactggag gctaaatgac
     8761 gccaggtgaa gtacggcgcc tatatttcat cattcgcact tttttaagct acggacttga
     8821 tgaactgatc cccaaaatgc gtatcaccct gccgctacgg ctatggcgat actcattatt
     8881 ctggatgcca aatcggcata aagacaaacc tttaggtgag cgactacgac tggccctgca
     8941 agaactggga ccggtatgga tcaagttcgg gcagatgtta tcaacccgcc gcgatctttt
     9001 tccgccgcat attgccgatc agctggcgtt attgcaggac aaagtcgctc cgtttgatgg
     9061 caagctggcg aagcagcaga ttgaagctgc aatgggcggc ttgccggtag aagcgtggtt
     9121 tgacgatttt gaaatcaagc cgctggcttc tgcttctatc gcccaggttc ataccgcgcg
     9181 attgaaatcg aatggtaaag aggtggtgat taaagtcatc cgcccggata ttttgccggt
     9241 cattaaagcg gatctgaaac ttatctaccg tctggctcgc tgggtgccgc gtttgctgcc
     9301 ggatggtcgc cgcctgcgcc caaccgaagt ggtgcgcgag tacgaaaaga ccttgattga
     9361 tgaactgaat ttgctgcggg aatctgccaa cgccattcag cttcgacgca attttgaaga
     9421 cagcccgatg ctctacatcc cggaagttta ccctgactat tgtagtgaag ggatgatggt
     9481 gatggagcgt atttacggca ttccggtgtc tgatgttgcg acgctggaga aaaacggcac
     9541 caacatgaaa ttgctggcgg aacgcggcgt gcaggtgttc tgaactcagg tctttcgcga
     9601 cagctttttc catgccgata tgcaccctgg caacatcttc gtaagctatg aacacccgga
     9661 aaacccgaaa tatatcggca ttgattgcgg gattgttggc tcgctaaaca aagaagataa
     9721 acgctatctg gcggaaaact ttatcgcctt ctttaatcgc gactatcgca aagtggcaga
     9781 gctacacgtc gattctggtt gggtgccacc agataccaac gttgaagagt ttgaatttgc
     9841 cattcgtacg gtctgtgaac ctatctttga gaaaccgctg gccgaaattt cgtttggaca
     9901 tgtactgtta aatctgttta atacggcgcg tcgcttcaat atggaagtgc agccgcaact
     9961 ggtgttactc cagaaaaccc tgctctacgt cgaaggggta ggacgccagc tttatccgca
    10021 actcgattta tggaaaacgg cgaagccttt cctggagtcg tggattaaag atcaggtcgg
    10081 tattcctgcg ctggtgagag cgtttaaaga aaaagcgccg ttctgggtcg aaaaaatgcc
    10141 agaactgcct gaactggttt acgacagttt gcgccagggc aagtatttac agcatagtgt
    10201 tgataagatt gcccgcgagc ttcagtcaaa tcatgtacgt cagggacaat cgcgttattt
    10261 tctcggaatt ggcgctacgt tagtattaag tggcacattc ttgttggtca gccgacctga
    10321 atgggggctg atgcccggct ggttaatggc aggtggtctg atcgcctggt ttgtcggttg
    10381 gcgcaaaaca cgctgatttt ttcatcgctc aaggcgggcc gtgtaacgta taatgcggct
    10441 ttgtttaatc atcatctacc acagaggaac atgtatgggt ggtatcagta tttggcagtt
    10501 attgattatt gccgtcatcg ttgtactgct ttttggcacc aaaaagctcg gctccatcgg
    10561 ttccgatctt ggtgcgtcga tcaaaggctt taaaaaagca atgagcgatg atgaaccaaa
    10621 gcaggataaa accagccagg atgctgattt tactgcgaaa actatcgccg ataagcaggc
    10681 ggatacgaat caggaacagg ctaaaacaga agacgcgaag cgccacgata aagagcaggt
    10741 gtaatctgtg tttgatatcg gttttagcga actgctattg gtgttcatca tcggcctcgt
    10801 cgttctgggg ccgcaacgac tgcctgtggc ggtaaaaacg gtagcgggct ggattcgcgc
    10861 gttgcgttca ctggcgacaa cggtgcagaa cgaactgacc caggagttaa aactccagga
    10921 gtttcaggac agtctgaaaa aggttgaaaa ggcgagcctc actaacctga cgcccgaact
    10981 gaaagcgtcg atggatgaat tacgccaggc tgcggagtcg atgaagcgtt cctacgttgc
    11041 aaacgatcct gaaaaggcga gcgatgaagc gcacaccatc cataacccgg tggtgaaaga
    11101 caatgaaact gcgcatgaag gcgtaacgcc tgctgctgca caaacgcagg ccagttcgcc
    11161 ggaacagaag ccagaaacca cgccagagcc ggtggtaaaa cctgctgcgg acgctgaacc
    11221 gaaaaccgct gcaccttccc cttcgtcgag tgataaaccg taaacatgtc tgtagaagat
    11281 actcaaccgc ttatcacgca tctgattgag ctgcgtaagc gtctgctgaa ctgcattatc
    11341 tcggtgatcg tgatattcct gtgtctggtc tatttcgcca atgacatcta tcacctggta
    11401 tccgcgccac tgatcaagca gttgccgcaa ggttcaacga tgatcgccac cgacgtggcc
    11461 tcgccgttct ttacgccgat caagctgacc tttatggtgt cgctgattct gtcagcgccg
    11521 gtgattctct atcaggtgtg ggcgtttatc gccccagcgc tgtataagca tgaacgtcgc
    11581 ctggtggtgc cgctgctggt ttccagctct ctgctgtttt atatcggcat ggcgttcgcc
    11641 tactttgtgg tctttccgct ggcatttggc ttccttgcca ataccgcgcc ggaaggggta
    11701 caggtatcca ccgacatcgc gagctattta agcttcgtta tggcgctgtt tatggcgttt
    11761 ggtgtctcct ttgaagtgcc ggtggcaatt gtgctgctgt gctggatggg gattacctcg
    11821 ccagaagact tacgcaaaaa acgcccgtat gtgctggttg gtgcattcgt tgtcgggatg
    11881 ttgctgacgc cgccggatgt cttctcgcaa acgctgttgg cgatccctat gtactgcctg
    11941 tttgaaatcg gtgtcttctt ctcacgcttt tacgttggta aagggcgaaa ccgggaagag
    12001 gaaaacgacg ctgaagcaga aagcgaaaaa actgaagaat aaattcaacc gcccgtcagg
    12061 gcggttgtca tatggagtac aggatgtttg atatcggcgt taatttgacc agctcgcaat
    12121 ttgcgaaaga ccgtgatgat gttgtagcgc gcgcttttga cgcgggagtt aatgggctac
    12181 tcatcaccgg taccaatctg cgtgaaagcc agcaggcgca aaagctggcg cgtcagtatt
    12241 cgtcctgttg gtcaacggcg ggcgtacatc ctcacgacag cagccagtgg caannnnnnn
    12301 nnnttgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    12361 accgcaactt ttcgacgccg gaagagcagg aactcgcttt tgttgcccag ctacgcattg
    12421 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    12481 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    12541 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    12601 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    12661 aattgctgat cgaaactgat gcgccgtatc tgctccctcg cgatctcacg ccaaagccat
    12721 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    12781 gtggagagga tgccgcatgg ctggctgcca ccacggatgc taatgtcaaa acactgtttg
    12841 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    12901 taataagatt aagcaatagc atggagcgag cctcgccgtc tggctcggtg aaaatggcct
    12961 gaaaaccttc gaatgcgcct tcagtaataa tcactttatc accaggatac ggcgtcgatg
    13021 gatcgacaat atctttcggt ttatacactg ataactgatg aatcaccgcc gatgggacta
    13081 tcgctggcga cgcgccaaag cgcacgaagt ggctgacacc gcgggtcgcg ttgatagtcg
    13141 tggtatgaat cacttccgga tcgaactcca caaacaagta gttggggaac aatggctcac
    13201 tgactgcagt acgtttgcca cgcacgattt tttccagggt gatcatcggt gccaggcaat
    13261 tcacagcctg tctttcgagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    13321 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    13381 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    13441 atttaacaaa tttacagcat cgcaaagatg aacgccgtat aatgggcgca gattaagagg
    13501 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cttgacgctg cttgaacagc
    13561 agggtgagct aaaacgtatc acgctcccgg tggatccgca cctggaaatc actgaaattg
    13621 ctgaccgcac tttgcgtgcc ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    13681 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    13741 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    13801 agccgccaaa aggtttccgt gacctgtttg ataaactgcc gcagtttaag caagtattga
    13861 acatgccgac aaagcgactg cgtggtgcac cctgccaaca aaaaatcgtc tctggcgatg
    13921 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    13981 ttacctgggg gctcaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    14041 atcgccagca gctaattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    14101 gcgcgttgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    14161 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    14221 tttcagagta tgcgtttgcc ggattgctgc gcggcaccaa gaccgaagtg gtgaagtgta
    14281 tctccaatga ccttgaagtg cccgccagtg cggagattgt gctggaaggg tatatcgaac
    14341 aaggcgaaac tgcgccggaa gggccgtatn nnnnnnnnnc gaccacaccg gttactataa
    14401 cgaagtcgat agtttcccgg tatttaccgt gacgcatatt acccagcgtg aagatgcgat
    14461 ttatcattcc acctataccg ggcgtccgcc agatgaacct gcggtactgg gtgtagcact
    14521 gaacgaagtg ttcgtgccga ttctgcaaaa acagttcccg gaaattgtcg atttttacct
    14581 gccgccggaa ggctgttctt atcgtctggc ggtagtgacg atcaaaaaac agtacgccgg
    14641 acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta cgccagttta tgtacactaa
    14701 atttgtgatc gtttgcgatg atgacgtcaa cgcacgcgac tggaacgatg tgatttgggc
    14761 gattaccacc cgtatggacc cggcgcggga tactnnnnnn nnnncccagc gtgaatgggg
    14821 acgtcccatc aaaaaagatc cagatgttgt cgcgcatatt gacgccattt gggatgaact
    14881 ggctattttt aacaacggta aaagcgcctg atgcgcgttt gttttgccct atttatcgat
    14941 ccgacagaga aagcgcatga caaccttaag ctgtaaagtg acctcggtag aagctatcac
    15001 ggataccgta tatcgtgtcc gcatcgtgcc agacgcggcc ttttcttttc gtgctggtca
    15061 gtatttgatg gtagtgatgg atgagcgcga caaacgtccg ttctcaatgg cttcgacgcc
    15121 agatgaaaaa gggtttatcg agctgcatat tggcgcttct gaaatcaacc tttacgcgaa
    15181 agcagtcatg gaccgcatcc tcaaagatca tcaaatcgtg gtcgatattc cccacggaga
    15241 agcgtggctg cgcgatgatg aagagcgtcc gatgattttg attgcgggcg gcaccgggtt
    15301 ctcttatgcc cgctcgattt tgctgacagc gttggcgcgt aacccaaacc gtgatatcac
    15361 catttactgg ggcgggcgtg aagagcagca tctgtatgat ctctgcgagc ttgaggcgct
    15421 ttcgttgaag catcctggtc tacaagtggt gccggtggtt gaacaaccgg aagcgggctg
    15481 gcgtgggcgt actggcaccg tgttaacggc ggtattgcag gatcatggta cgctggcaga
    15541 gcatgatatc tatattgccg gacgttttga gatggcgaaa attgcccgtg acctgttttg
    15601 cagcgagcgt aatgcgcggg aagatcgcct gtttggcgat gcgtttgcat ttatctgaga
    15661 tataaaaaaa cccgcccctg acaggcggga agaacggcaa ctaaaacttt attcagtggc
    15721 atttagatct atgtcgcatc cggcaattgg tgcacgatgc ctgatgcgac gctggcgcgt
    15781 cttatcaggc ctacattggt gccggatcgg tagaccggat aaggcgttca cgccgtatcc
    15841 ggcatacagt gcccgatgcg acgctgccgc gtcttatcgg gcctacaaaa gttctgaacc
    15901 gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc tcaaacaccg
    15961 ttgcaatgcc ctgacccaga ccgatacaca tcgtcgccag accaaactgg acgtctttgt
    16021 gttccatcag attcagcagc gtggtgctga tacgcgcacc ggaacaaccc agcggatgac
    16081 ccagcgcgat cgcgccaccg ttgaggttga tcttctcgtc aatctgctct attagcccga
    16141 gatctttaat acacggcagg atctgcgcgg caaaggcttc gttcatctcg aatacgccga
    16201 tatcgctggc agaaagcccc gcttttttca gcgccagctt cgaggccgga accggaccat
    16261 aacccataat agatgggtca caacccacaa ccgccatcga acgcacgcgg gcgcgcggct
    16321 taagacctaa ttcacgggcg cggctttcac tcatcaccag catggcagct gcgccatcgg
    16381 aaagcgcaga agatgtaccc gccgttaccg taccgctgac gggatcaaac gccggacgca
    16441 gtgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa ttaaactgct
    16501 tcaatacgcc gtcggcatcg tgaccaccgg tcgggatgat ttcattttta aatgcgcctg
    16561 actgcgtggc ggcccacgcg cgggcgtgtg accgcgcggc aaaggcatcc tgcatttcac
    16621 ggctgatacc atgcatacgc gccagcattt ctgccgttaa gcccatcatg cccgccgctt
    16681 tggcgacatt acggctaagg ccggggtgaa aatcgacgcc gtgactcatt ggcacatggc
    16741 ccatatgctc cacgccgcca accagacatg cctgcgcatc gccggtcatg atcattcgtg
    16801 ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg attaacggta accgccggga
    16861 cagagtgcgg cacttcagcc aacagtgccg cgttacgggc gatgttaaaa ccctgctcca
    16921 gtgtttgctg cacacaaccc cagtaaatat cgtcgagggc cgctgcttcc agcgccgggt
    16981 tacgcgccag caggctacgc attaaatgag cggagagatc ttccgcacgc acgttacgaa
    17041 aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg acaacctgtt
    17101 ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc tcaaccgggg
    17161 gatagtacgg ttcgttatga cgtgctttat tacgcagacc ttccggtact tcatacagcg
    17221 ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctgccg agggtgtcca
    17281 gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc agcgccatat
    17341 ccgcttctgc cggagtggcg ataatgccct cttccagaca gcggaccact tcgttgacca
    17401 tcgggatcat catgcgggcg ataatctctt cctcgctgaa atcgcgcttc ggctggctca
    17461 cttttgccag caggtcgtca acgacgacat cttcttcttt cttcggctta cctttgcttt
    17521 ataacgccag aaaccgaggc cgttcttctg accaaagcgg ttggcatcaa acagcgcgtc
    17581 gatggcatcg cgataatctt tctgcatccg ctgcgggaag cctgctgcca tgacagcctg
    17641 agcgtgatgc gcggtgtcaa tgcccacaac atccagcaga tatgccggac ccatcggcca
    17701 gccaaactgt ttttccatca ctttgtcgat cttgcggaaa cccgcgccgt cgcgcagcaa
    17761 ctggctgaaa ccggcgaaat aggggaacag cacac
//
LOCUS       DAWDRC010000016        17067 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1823_length_17067_cov_2.516577, whole genome shotgun sequence.
ACCESSION   DAWDRC010000016 DAWDRC010000000
VERSION     DAWDRC010000016.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 17067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_17067_cov_2.516577"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    703..712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1142..1151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1603..1612
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4707..4716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5989..5998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10250..10259
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10979..10988
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4000 a         4276 c         4538 g         4183 t
ORIGIN      
        1 tcgtctgaac cttcagtgat attccagacg cgttcagcat attgcagcag cttagcgcgc
       61 ttggtatcgc gtttttcatt ccacagtgca ggcaggacga tagccagtgt ttgcgcgtga
      121 tccagaccgt gcatcgcagt cagttcgtgg cccagcatat gcgttgccca gtcctgcggt
      181 acgccagcgc cgatcaaacc gttcagcgcc tgagtcgccg cccacatgac attggcgcgc
      241 acatcgtagt tttctggctc tttcagggct ttcggaccat cttcgatcag cgtcagcaaa
      301 atgccttctg cgaaacggtc ctgaattttg gcatcaaccg gtttggtaac atactgttcc
      361 acggtgtgta caaaggcgtc cactacgccg ttagccacct gacgcgacgg cagggtgtag
      421 gtataaaccg gatcgagcac ggcaaatacg ggctgaacat gggcagaatg gaacgcctgc
      481 ttgtcgcctg tggttttacg ggagatcacc gcgcctgcgt tggattctga accggttgct
      541 ggcagcgtca gcacacagcc catcgggatg gcgcttttaa tctctttgcc gcccgtttgc
      601 agaatgtgcc acggatcgat attttccgga tagttagccg ctgcggcgat aaatttggtg
      661 ccgtccagta cagaaccgcc gccaaccgcc agcagaaaag tcnnnnnnnn nncagtttca
      721 cggcgttcat cagcgtttca taagccgggt ttggctcaat accgccaaat tccagtacgt
      781 ccatgccttt cagggcatcc agaacttgat cgagaacgcc ggtttttttc acgctgccgc
      841 cgccgtaggt aatcaatacg cgagcatcgt gaggaatttg ttcgcgtaaa ccagcgattg
      901 cgcctttacc aaacagaatg cgggttgggg tgtgcagatt aaagttgttc attacttgct
      961 ccctttgctg ggtcaatatg agggcagaga tcggtctgcc tgatgttttt cattgtggtc
     1021 gccagcgccg tacctctcaa tgctcatttc tgccaatgtc ttgcctattt ctccagagtg
     1081 ctggagaaat gctacaaaat tgcgcacaat caaattgccg cattattcct aagaaattac
     1141 gnnnnnnnnn ntacgcgata tgaaacgtga agagatttgc cgcttgctgg cggataaagt
     1201 taataaactg aaaaataaag aaaatagttt gtcagagctg ttgcccgatg tgcgtttgtt
     1261 gtatggcgag acgcctttcg cacgcacacc ggtgatgtac gagcctggca tcataattct
     1321 cttttccgga cataaaatcg gttatatcaa tgaacgcgtg tttcgttatg acgctaatga
     1381 atatctcctg ctgacggtgc cgttgccgtt tgagtgcgaa acctatgcca cgtcagaggt
     1441 gccgctggca gggttgcgtc tcaatgtcga tattttgcag ttacaggaac tgttgatgga
     1501 cattggcgaa gatgagcatt tccagccgtc gatggcagcc agcgggatta actctgccac
     1561 gttatcagaa gagattttat gcgcggcgga gcggttactg gannnnnnnn nnatgtgatg
     1621 gagcgaccgc tggatgcgcg tattctcggc aaacagatca tccgcgaaat tctgtactac
     1681 gtgctgaccg gaccttgcgg cggcgcgtta ctggcactgg tcagccgcca gactcacttc
     1741 agcctgatta gtcgcgtgtt gaaacggatt gagaataaat acaccgaaaa cctgagcgtc
     1801 gagcaactgg cggcagaagc caacatgagc gtatcggcgt tccaccataa ttttaagtct
     1861 gtcaccagca cctcgccgtt gcagtatttg aagaattacc gtctgcataa ggcgcggatg
     1921 atgatcatcc acgacggcat gaaggccagc gcggcggcga tgcgcgtcgg ctatgaaagc
     1981 gcatcgcaat ttagccgtga gtttaaacgt tacttcggtg tgacgccggg ggaagatgcg
     2041 gcaagaatgc gggcgatgca ggggaattaa atacgttccc ggtacagcaa ccgggaacgg
     2101 ggaaaatcgt caggcgttac agtatttttt tttaatcacc acaaacagcg tgcccaacaa
     2161 gccagcggtt aacaaggcaa ttggcaggat catcaggaac gtcattacct gatcttcatg
     2221 tcgtttaacg aacggaatca tacttaaggc atagccgaaa ctggttacca cgctgaccca
     2281 cagcaatccg cttaaccagt tgaaaaactg gaagcggcgg tttggcagac cggaaattcc
     2341 cgccatggtt ggcagcagcg tacggacaaa tgcaagaaaa cgtccagcca gcagcgccag
     2401 cagaccgtgg cggtcaaaca tgcaggtggc gcgctggtga tatttagcag gaagctgtgc
     2461 cagccagcct ttcaccgttt tggtattccc taaccagcgc ccctgaatat aacttagcca
     2521 gcagcccaga cttgctgcgg cggtcagaat cgcaatcgta ggcagaaaat ccatagcccc
     2581 atgagcaatc aatgcgcctg ccagtatcaa caagctgtcg cctggcaaaa atgaggcggg
     2641 cagcaagccg ttttctaaaa acagcgtggc aaacataaca aagtaaacaa cgctaacaat
     2701 atgagggtcc gccagcgcgg caaagtcgtg ttgccagagc gcagcgatga tatcttgaat
     2761 aacagccatg gactttcctg tggaacagcg tttaagcctt tatctgccta ttgtactcct
     2821 gaatcgtccg ggacgccttg atcccggacg caacaattta agacttttca caataaaatg
     2881 tctgcaaaat tgtccaaaag tggcaatgtt atacaattcg cgcaaaaccg gcgtccagat
     2941 cggcaatcag atcgtcgaca tcttccagac caatatgcag gcgaatcaag gtcccgctaa
     3001 aatcgatctt gccttgtggg cgaatggcgg cgatatgttc tggttgattt gccaggatca
     3061 acgattcata cccgccccac gagtaggcca tgctgaataa actgaagtta tccagatagt
     3121 tcgccagctc ttcatcattg agttttttct taagcacaaa ggaaaatagc ccgctgctgc
     3181 ctgtaaagtc tcgtttccag aattcgtggc ctttactgcc aggcagagca gggtggttaa
     3241 ctcgcgctac ttggggatgt tctgccagcc attcagccac tttcagactg ctttcatgat
     3301 gttgacgcag gcgaaccccc aaagtgcgca ggccacggct ggttatatag gcggtatcgg
     3361 catcgaccat ctggcccatc agataggcat tctctcgcag ctgctcccag caacgggcat
     3421 tgcacacggc agtgccaatc atcgcatctg aatgcccaac cagatatttg gtggcggcct
     3481 gaatagaaac atcgatgcca aaatccagcg ccttaaacag cacaccggct gcccaggtgt
     3541 tgtcgatcat aatgatggca tccgggacca cactgcgtac ggcggcaaca atcgccggaa
     3601 catcgtggac ttccatggtg atggaacctg gcgattccag aaacacgatt ttagtgtttg
     3661 gctgcagatg attaacgata tcggcaccaa tcagcggatc aaaccacgat gtcgttacgc
     3721 ccagtttgct gaggattttg ctacagaaat cctgactcgg ttcataggcg gtgttggtca
     3781 tcaacacatg atcgccctgt tcgacaaagg caagattgga attagcaacc gcggccgccc
     3841 cgcagggaaa tagcacacag cctgcgccac cttccagttc acacatcgct tgttgtaagg
     3901 agaaatgggt taacgttcca cgccgtccat agaacaactc gccattggcg cgattacgtg
     3961 tcgcgtgttt tttggcttcc acactgtcaa agaccagcga agacgcgcgc tgaatcacgc
     4021 tatttaccgc accgagcgtg tattttttgc tgcgtcctgc attcaccagt tgagtatcaa
     4081 gctttttgtc cgccatgtcg ggattcctgt ttttatacgt ctggatgtct aaactagcat
     4141 gaatattcgc gggcgcatcc tgaagaattg gcataaagca gaaaattttc gcaaatggcg
     4201 tgctggttac ttttttactg cgtaaacgca aggaaaagaa agcaaaggta cggcagtatg
     4261 caaatagtaa tgagaacgac tatcaattcg acgtcgtttt gatattatta tgcacagatt
     4321 ttgtgacttg cgtcctggag atacacagtg ggtaataatt taatgcagac ggacctctcc
     4381 gtctggggta tgtatcagca cgccgatatt gtcgttaagt gcgtgatgat tgggcttatt
     4441 ttggcctccg tagtcacctg ggcaattttc ttcagtaaga gcgtggagtt cttcaatcag
     4501 aagcgtcgtc ttaagcgcga gcagcaactg ctggctgaag cgcgttcctt aaaccaggcc
     4561 aacgatatcg ccgctgattt tggtagcaaa agcttgagcc tgcatttgct caatgaagcg
     4621 cagaatgagc tggaactgtc agaaggcagc gacgataacg aaggtattaa agaacgtacc
     4681 agcttccgtc tggagcgtcg ggtcgcnnnn nnnnnncggc gcgatttcgc cgttcgttgg
     4741 tctgtttggt acggtctggg gcatcatgaa gagctttatt ggtatcgcgc aaacgcagac
     4801 cactaacctg gcagtcgttg cgccaggtat tgcagaagct ctgttagcaa cggcaatcgg
     4861 cctcgtggca gcgattcctg cggtcgttat ctataacgta tttgcacgcc agattggcgg
     4921 ctttaaagcg atgctgggtg atgttgcagc gcaggtattg ttgctgcaaa gccgtgacct
     4981 ggatctggaa gccagcgccg ctgcgcatcc ggttcgtgtc gcacaaaaat tacgtgcagg
     5041 ataatgtccg atggcgatgc atcttaacga aaacctcgac gataacggcg aaatgcatga
     5101 tatcaacgtg acgccgttta tcgacgtgat gttggttttg ctgattatct ttatggtggc
     5161 ggcaccgtta gcgacggtag atgtgaaggt gaatttgcct gcttctacca gcacgccgca
     5221 gccgcgcccg gaaaaaccgg tttatctgtc ggtgaaagca gacaactcga tgtttattgg
     5281 taacgatccg gtcaccgatg aaacaatgat tacggcgttg aatgcgttaa ccgaaggcaa
     5341 gaaagacacc accatcttct tccgtgcgga taaaactgtc gattacgaga cgttgatgaa
     5401 ggtaatggat acgctgcatc aggcgggtta cctgaaaata ggtctggtcg gcgaagaaac
     5461 tgcgaaagcg aaataaaaat gatgcccggt tgcttttcac aaccgggcat ttttttaacc
     5521 taaatgctcg ccgccgcaga caccgtgcac ttctgcggtg acgtagctcg actcctgact
     5581 tgccaggtaa acatatacag gggccagctc cgccggttgc cccgcacgct tcatcggcgt
     5641 tttctgacca aactgcggga tcttatcctg cgtttgtccg ccagaaattt gcagtgctgt
     5701 ccagatcggg cctggcgcga caatattcac ccgaatacct ttctccgcga cctgttttgc
     5761 caggccacgg ctgtagttca gaattgccgc cttcgtagcc gcatagtcca gtaaatgcgg
     5821 gcttggctgg tatgcctgga ttgaggaagt ggtgatgata cttgcacctt tcggtagcag
     5881 ggggatcgct tcctgggtta gccagaacaa cgcgaaaacg ttaatggcaa aggtcttttg
     5941 aaactgttcg ctggtgaggt ctgcaatatc aggaatggca acctgtttnn nnnnnnnnta
     6001 ttatccagcc cgcctaacgc cttgtgtgct tcgtgaacca gcgaacgggc gaatttctca
     6061 tcgcttaaat cgcctggcag cagaacggct ttgcgtccgc attcttcaat gatctttttc
     6121 acatcctgag cgtcttcttc ttccacggga agataactga tcgccacgtc agccccttca
     6181 cgcgcgtaag caatggcggc agcgcgaccg attccggaat cgccccctgt caccagtgct
     6241 ttacgagctt tcaggcgacc gctaccaaca taggttttct cgccgcaatc cggtaccggt
     6301 gtcatcttcg cctggatgcc tggcgtcggt tgtttctgtt tgggatattc accagtgtaa
     6361 tactgcgtgg tcgggtcttt taaatgagac atcgtttttc tcccttcagg ttcaacgtcc
     6421 tttaagggta gacgctctcg atgcgttgat aagggaacca ggaagatccc taaccctcag
     6481 aattatgcga caaaggttta acgtatatgt tgatttgctg ttgcgtgctg tttacttaat
     6541 tgcgatatac tgttgcccgt tttaactaca cgacaggaat gtatggaacg ttttcttgaa
     6601 aatgcaatgt atgcttctcg ctggctgctt gcccccgtgt actttggcct ttcgctggcg
     6661 ttagttgccc tggcgctgaa gttcttccag gagattattc acgtactgcc gaatatcttc
     6721 tcgatggcgg aatcagattt gatcctcgtg ttgctgtcgc tggtggatat gacgctggtt
     6781 ggcggtttgc tggtgatggt gatgttttcc ggttacgaga atttcgtctc acaactcgat
     6841 atctccgaga acaaagagaa gctgaactgg ctggggaaaa tggacgcaac gtcgctgaaa
     6901 aacaaagtag cagcgtcgat tgtggcaatt tcttccattc acttactgcg cgtctttatg
     6961 gatgcgaaaa atgtccctga taacaaactg atgtggtacg tcattatcca tctgacgttt
     7021 gtgctctctg catttgtgat gggctatctt gaccgactga ctcgtcataa tcactgatct
     7081 gattcgggcg cggttctcgc gcccgttatt aacaggtcat ttatcggaag acgcctgcca
     7141 cagattcagc tctccatcgg cgatatgctg atcaatctgc gccagctcct cggtgctaaa
     7201 tgtcagatta ttcagcgcct gcacgttctc ctcaagttgc tccgcgcggc tggcaccaat
     7261 caataccgac gtcactcgct catctttcag caaccagctt aacgccattt gcgccattga
     7321 ttgtccacgc tgctgtgcca tttcatgcaa taagcgcagg ctgttgaggt tggcttcggt
     7381 gagcattttc ggcgtcagac cacgaacttt attcccttca cgatgcatcc gtgaatcttg
     7441 cggaataccg ttgagatatt ttccggtcag caatccctga gccagtggag taaaggcaat
     7501 gcagcccacg ccgttatttt gcagggtatc cagcaggccg cttttatcca cccagcggtt
     7561 cagtaaattg tacgaaggtt gatgaattaa cagcggaatt ttccactcgc gcagcaactc
     7621 gaccattttt tgcgtccgct ctggcgagta agaggagatc ccgacataca gcgccttacc
     7681 gctttgtaca gcatgagcca gcgcagaggc ggtttcttcc atcggcgtat tttcatcgac
     7741 gcgatgagag taaaagatat cgacatactc aagccccata cgcttcaggc tttggtcgag
     7801 gctggcgagc aggtatttac gtgagccgcc agagccgtat ggaccgggcc acatatcgta
     7861 tccggctttg gtagagataa tcagttcatc gcgataagcg gcaaaatcct cgcgcagcag
     7921 gcgaccaaag ttttcttctg cgcttcctgg aggcggcccg taattgttgg ctaaatcaaa
     7981 gtgcgtaatg cctaaatcaa acgctttacg caggatcgca cgctgtgatt ccagcgcgtt
     8041 aacgtgaccg aaattgtgcc ataaaccgag cgataacgcg ggcaggcgta aaccactttt
     8101 tccgcaatag cggtactgca tccgcccgta acgttcggga ttcgctaacc agaccatgac
     8161 ctctcctttc caccattcaa tttcgaaaca atgtttctag tttagcgatt cgccagcgcg
     8221 tatcccgtag tctggctcac agagtgacga aaaactgggc aaaaacacgc gcttatgctt
     8281 tgcttaaaaa aacaccagtt gaggagtgca acgatgccgc gtttaaccgc caaagatttc
     8341 ccacaagagt tgctggatta ctacgactat tacgctcacg ggaaaatctc gaaacgtgag
     8401 ttcctcaacc ttgcggcgaa gtatgcagtg ggcgggatga cggcattagc gctgtttgat
     8461 ttgctcaagc caaattatgc gctggcgact caggtagagt ttaccgaccc ggaaattgtt
     8521 gcagagtaca tcacgtatcc ctcgccaaat ggtcacggcg aggtacgggg ttatctggtg
     8581 aagcccgcaa aaatgagcgg caaaacgcca gccgtggtag tggtgcatga gaatcgaggg
     8641 ctgaatccgt atatcgaaga tgtgacgcgg cgagtggcga aggcggggta catcgccctg
     8701 gcgccagacg gtttaagctc agtaggtggc tatcctggta acgatgataa aggccgcgaa
     8761 ctgcaacagc aggttgatcc aaccaaactg atgaatgatt tctttgccgc aattgagttt
     8821 atgcaacgct atccgcaagc gacaggcaaa gtgggtatta gcggattttg ctatggcggg
     8881 ggcgtatcga acgcggcagt agtcgcgtat ccggaactgg cctgcgcggt gccgttttat
     8941 gggcgtcagg cacccactgc cgatgtggcg aagattgaag cgcctttact gctccactac
     9001 gctgaactgg atagccgaat caacgagggc tggcccgctt acgaggcggc gttgaaagcc
     9061 aataataagg tctatgaggc gtatatatat ccggaggtta atcacggatt ccataatgat
     9121 tccacgcccc gttatgacaa atctgccgcc gatcttgcct ggcaaaggac gctgaaatgg
     9181 ttcgataaat atctctcctg atgggtttat ctcttacagg attacgtctt aaacaagcat
     9241 gaaaaaatag cgtgcgcaaa tgtcgttctt tgcctaaaat atcgctatat atgacaatat
     9301 ataacgaatg aggtgaacga tgaataacca ttttggtaaa ggcttaatgg cgggattaaa
     9361 agcaacgcat gctgacagtg cggttaatgt gacaaaattc tgtgccgatt ataaacgcgg
     9421 ttttgtatta ggctactcac accggatgta cgaaaagacc ggagatcgcc agcttagcgc
     9481 ctgggaagcg ggtattctga cgcgccgtta tggactggat aaagagatgg taatggattt
     9541 ctttcgtgag aataattcct gttctacgtt gcgctttttt atggccggtt atcgcctaga
     9601 aaattgatca aacatacgta ttatcttgct ttaattaatt acactaatgc ttcttccctt
     9661 cgttttagtg ccccgccgca gtatcatgat atcgataacc ataataaatg tgtggtaaat
     9721 ggcgcatcga tcgcattatt gattttgcga ttgaggcaaa atatatgcca ggtcttcgca
     9781 acggaataac tataaatgac tggagataac accctcatcc attctcacgg cattaaccgt
     9841 cgtgatttca tgaagctttg tgcagcatta gccgccacca tggggttaag tagcaaagcc
     9901 gctgcagaga tggccgaatc ggttactaac ccgcagcgtc cgccagttat ctggattggc
     9961 gcgcaggagt gcaccggttg tacggaatct ctgcttcgtg caacgcatcc aacggtagaa
    10021 aacctcgtgc tggagactat ctctctggag tatcacgaag tgctttccgc cgccttcggt
    10081 catcaggtcg aagagaacaa acataacgct ctcgagaagt acaaagggca gtatgtgtta
    10141 gtggtggatg gttccatccc attaaaagat aacggtattt attgcatggt tgccggtgag
    10201 ccgattgtgg atcacatccg caaagcggcg gaaggcgcag cagccattan nnnnnnnnng
    10261 ggcaaaaccg ttatcaatat tcctggctgc ccgccgaacc cgcacaactt cctcgcgacc
    10321 gttgcgcaca tcatcactta cggcaaaccg ccgaaactgg atgacaaaaa ccgtccgact
    10381 ttcgcctatg gtcgtctgat tcacgaacac tgtgaacgtc gcccgcactt cgatgctggt
    10441 cgttttgcca aagagttcgg tgatgaaggt caccgcgaag gctggtgcct gtaccacctc
    10501 ggctgtaaag ggccagaaac ttacggcaac tgctcaacgc tgcaattctg cgatgttggc
    10561 ggcgtgtggc cggtagcgat tggtcacccg tgctatggct gtaacgaaga aggtatcggc
    10621 ttccataaag gcatccatca gcttgccaac gtcgaaaacc agactccgcg ttcacagaaa
    10681 ccggacgtta acgctaaaga aggcggcaac gtctcggcag gcgctattgg tttgctcggc
    10741 ggagtggttg ggctggttgc cggtgtcagc gtgatggcgg tgcgtgaact gggtcgtcag
    10801 caaaagaaag ataacgctga ctcacgggga gaataaccgt gaacagacgt aattttatta
    10861 aagcagcctc ctgcggggca ttgctgacgg gcgcgctgcc gtctgtcagt catgcggctg
    10921 ctgaaaaccg cccgccaatt ccgggatcgc tggggatgtt gtacgactcg accttgtgnn
    10981 nnnnnnnntt gtgcgtaggc tgtcaggctt gcgtcaccaa gtgtcaggat atcaacttcc
    11041 ctgaacgtaa cccgcaaggg gaacagacct ggtcgaacaa cgacaaactg tcgccgtata
    11101 ccaataacat cattcaggtg tggaccagcg gcacaggggt caacaaagac caggaggaga
    11161 atggctacgc gtacattaag aaacagtgta tgcactgcgt cgatccgaac tgtgtctctg
    11221 tgtgcccggt ctctgcgctg aaaaaagatc cgaaaaccgg cattgtccat tacgacaaag
    11281 atgtgtgcac cggctgccgt tactgcatgg tcgcctgccc atacaacgtg ccgaagtacg
    11341 actacaacaa cccgtttggt gcgctgcata agtgcgagtt gtgcaaccag aaaggtgtgg
    11401 aacgtctcga taaaggcggt ctgcctggct gtgtagaagt gtgcccggcg ggcgcggtga
    11461 tttttggtac gcgtgaagag ctgatggcgg aggcgaaaaa acgtctggcg ctgaagccgg
    11521 gcagcgaata ccactatccg cgtcagacgc tgaaatctgg cgacacttac ctgcatacgg
    11581 tgccgaaata ttatccgcat ctgtacggcg agaaagaggg cggcggtact caggttctgg
    11641 tactgacggg tgtgccttat gaaaatctcg acctgccgaa actggacgat ctttctactg
    11701 gtgcgcgttc cgaacatgtc caacacaccc tgtataaagg catgatgcta ccactggctg
    11761 tgctggcggg cttaaccgtg ctggttcgtc gcaacactaa aaacgaccat cacgacggag
    11821 gagacgatca tgagtcatga tccacaaccg ctgggcggca aaatcatcag taaaccggtc
    11881 atgatttttg gaccgttaat cgtcatctgt atgctcctga ttgtgaagcg tctggtgttc
    11941 ggtctgggct ctgtctccga cctgaacggc ggcttcccgt ggggcgtgtg gatcgcgttt
    12001 gacctgctga tcggtaccgg ctttgcctgt ggcggctggg cgctggcgtg ggcagtatac
    12061 gtctttaacc gtgggcaata ccatccgctg gtgcgtccgg cgctgttggc gagtctgttt
    12121 ggttattcac tgggtggctt gtcgatcact atcgacgtgg gtcgctactg gaacctgccg
    12181 tacttctaca ttccgggtca cttcaacgtg aactcggtac tgttcgagac ggcggtctgt
    12241 atgaccatct acatcggtgt gatggcactg gagtttgctc cggcattgtt tgaacgtctg
    12301 ggctggaagc tgtcactcaa acgtctgaac aaggtgatgt tctttatcat cgcgcttggt
    12361 gcgctgctgc caactatgca ccagtcttca atgggatcgc tgatgatctc ggcgggctac
    12421 aaggtgcatc cgctgtggca aagctatgaa atgttgccgc tgttctcgct gctgacggcg
    12481 ttcatcatgg gcttctcgat tgtcatcttt gaaggttcgt tggtgcaggc gggtctgcgt
    12541 ggcaacggtc cggatgaaaa gagtctgttc gttaagctga ccaataccat cagtgtgttg
    12601 ctggcgattt tcatcgtgct gcgctttggc gagcttatct atcgcgacaa gctgtcgtta
    12661 gcgtttgccg gtgacttcta ctccgtgatg ttctggattg aagtcctgct gatgctcttc
    12721 ccgctggtcg ttctgcgtgt ggcgaagttg cgtaatgatt cccgcatgct gttcctgtca
    12781 gcactgagtg cactgttagg ttgtgcaacc tggcgtctga cctattcgct ggtggcattc
    12841 aacccgggtg gcggttacgc ctacttcccg acctgggaag aactgttgat ttctattggt
    12901 tttgtggcta ttgagatttg cgcttatatc gtactcattc gtctactgcc gatacttcct
    12961 cctttaaaac aaaacgatca taatcgtcat gaggcgagca aagcatgagc cagagaatta
    13021 ctattgatcc ggtaacccgt attgaagggc atttacgcat cgattgcgaa atcgaaaatg
    13081 gcgtcgtttc gaaagcatgg gcttccggta ccatgtggcg cggcatggaa gagatcgtga
    13141 aaaaccgcga tccgcgcgat gcatggatga ttgtgcaacg tatctgtggc gtatgtacta
    13201 ccactcacgc gctgtcttcc gttcgtgcgg cagaaagtgc gctgaatatc gacgttccgg
    13261 ttaacgcgca atacatccgt aacatcattc tggctgcgca caccacgcat gaccatattg
    13321 ttcatttcta tcagctttcg gcgctggact gggtggacat cacttctgca ctgcaagccg
    13381 atccgaccaa agcctccgaa atgctgaaag gcgtttcgac ctggcatctg aacagcccgg
    13441 aagagttcac caaagttcag aacaagatca aagatctggt tgccagcggt cagttgggta
    13501 ttttcgccaa tggctactgg ggtcacccgg caatgaaact gccgccggaa gtgaacctga
    13561 ttgcggtagc gcactacctg caggcgctgg agtgccagcg tgacgctaac cgcgttgtgg
    13621 cgctgctggg cggtaaaacg ccgcatattc agaacctggc ggtaggtggt gtcgcgaacc
    13681 cgatcaacct cgacggtctg ggtgtgctga accttgagcg cctgatgtac atcaagtcct
    13741 tcatcgacaa gctgagcgac tttgttgagc aggtttacaa ggttgatact gcagttatcg
    13801 ctgcgttcta cccggaatgg ctgacgcgcg gtaaaggtgc ggtgaactac ctgagcgtgc
    13861 cggaattccc gaccgacagt aaaaatggca gcttcctgtt cccgggcggc tacattgaga
    13921 atgcggatct gtcctcgtat cgtccgatca cttctcattc cgatgaatac ctgatcaaag
    13981 ggattcagga aagcgcgaag cactcctggt ataaagacga agcgccgcag gcaccgtggg
    14041 aaggtaccac cattccggct tatgatggtt ggtctgacga cggtaaatat tcctgggtga
    14101 aatcaccgac tttctacggc aaaacggtag aagtgggtcc gctggctaat atgctggtga
    14161 aactggcggc aggacgcgag tctacccaga acaaactgaa tgaaatcgtt gcgatttatc
    14221 agaaactgac tggcaacacg ctggaagtgg cgcagttgca ctccacgctg ggccgtatta
    14281 ttggtcgtac cgttcactgc tgtgaattgc aggatatcct gcaaaatcaa tacagtgcac
    14341 tgatcaccaa tatcggcaaa ggcgatcaca ccacctttgt gaagccgaac attccggcaa
    14401 cgggtgagtt caaaggcgta ggcttcctcg aagcaccgcg cggtatgctc tctcactgga
    14461 tggtgatcaa agacggtatc atcagcaact accaggcagt tgttccatca acctggaact
    14521 ctggcccgcg taacttcaat gatgacgtcg gtccttacga gcagtcgctg gtgggtacac
    14581 cggttgccga tccgaataaa ccgctggaag tggtgcgtac cattcactcc ttcgacccgt
    14641 gcatggcctg tgcggtacac gtagtggatg ccgacggcaa cgaagtggtt tcagtgaagg
    14701 ttctgtaatg cgtattttag tcttaggggt cggcaatatt ttgctgaccg atgaagccat
    14761 cggtgtgcgg attgttgaag cgttagagca acgatacatt ctgccggatt atgttgagat
    14821 cctcgatggc ggcacggcgg gaatggagct gcttggcgac atggcaaatc gcgatcatct
    14881 gattattgcg gatgccattg tgtcgaaaaa gaacgcaccg ggaacgatga tgatcctgcg
    14941 ggatgaagaa gttccggcgt tgtttaccaa caaaatctct ccgcatcagc ttggcctggc
    15001 cgacgtattg tcggccctgc gcttcaccgg cgagtttccg aaaaagctga ccctggtcgg
    15061 cgtgatcccg gaatcactgg agccacacat cggtttgaca ccgacggtcg aagcgatgat
    15121 tgaacctgcg cttgagcagg ttctggctgc gctgcgcgaa tcaggcgtgg aagctatccc
    15181 acgggaggcg actcatgact gaagagatag caggtttcca gacctccccg aaggtgcaag
    15241 tacaggcagc ttttgaagaa attgcccggc gttcgatgca tgatctctct tttctgcatc
    15301 cttcaatgcc ggtgtatgtt tctgacttta cgctgttcga aggtcagtgg acggggtgtg
    15361 tgatcacccc gtggatgctg agtgcagtta tcttccccgg cccggatcaa ctctggccga
    15421 tgcgcaaagt gagtgaaaaa attggtctgc aactgccgta tggcactatg acctttactg
    15481 ttggtgaact ggatggtgtt tcgcaatacc tctcctgctc gctgatgtcg ccgctttcgc
    15541 acagcatgtc gattgaagag ggccaacggc tgacggatga ctgcgcccga atgatccttt
    15601 cgctgccagt caccaatccg gatgtaccac acgcagggcg tcgtgccctg ctatttggcc
    15661 gcaggagtgg cgaaaatgca tgagttgtcg ctttgccaga gcgccgttga aattatccaa
    15721 cggcaggcgg agcagcacga tgttaagcgc gtcaccgccg tgtggctgga aattggtgca
    15781 ctctcctgcg ttgaggagag cgccgtccgt tttagttttg aaattgtctg ccacggaacg
    15841 gtagcgcaag ggtgcgattt acatatcgtc tataaacccg cccaggcctg gtgctgggat
    15901 tgcagccagg tggtggagat tcatcagcac gatgcgcagt gtccgatctg tcatggcgag
    15961 cggttgcgtg tcgataccgg cgattcgcta atcgtcaaaa gtattgaagt tgaataaccg
    16021 gagttaacaa tgtgtattgg cgttccaggc caggtgctgg ctgtcggtga agatattcac
    16081 cagcttgcgc aggttgaagt atgtggtatc aagcgcgacg tgaatatcgc cctgatttgt
    16141 gaaggtaacc ctgccgatct gctgggccag tgggtactgg tacacgtcgg atttgccatg
    16201 agcattatcg atgaagacga agccaaagcc acattagacg cactgcgcca aatggattac
    16261 gacattacca gcgcgtgatg attagctttt gtaggcctga taagacgcgg cagcgtcgca
    16321 tcaggcattg tgctcgattg ccggatgcgg cgtgaacgcc ttatccggcc tacattggtc
    16381 tggatggctt gctgcgacga acgctaacca ttacccctga cgcttatctt ccgtattcgt
    16441 ctcgaaatca ctggcgtcat ggcgctcatg caactgctcg ttcagcggcc cgttggtgcg
    16501 gttaacaata cgcccacgct tcactgccgg acgttcgccc acttctttcg cccagcgttg
    16561 tacatgctta taactgcccg catcaagaaa ctctgcggca tcatatacgc cgcctaacac
    16621 cacattgcca aaccatggcc aaatcgccat atccgcaatg gtgtactcat cgcccgcaac
    16681 aaacttatgc tgcgccagtt gcttatccag cacgtcgaga aggcgcttgg cttccatagt
    16741 aaagcggttg atggcgtact caattttcac cggtgcgtaa tggtaaaagt gaccaaaacc
    16801 accgccgagg aacggtgccg cgccctgtaa ccagaacagc cagctcattg cttcagtacg
    16861 tttcgccaga tcctgcggca agaagtagcc aaatttctct gccagataaa gcagaatcga
    16921 tccagactca aacacgcgga tcggcggatt atgggtatga tcgcgcaatg ccgggatctt
    16981 cgaatttggg ttcacttcga caaagccgct ggagaattga tcgccatcgc caatacgtat
    17041 cagccaggcg tcgtactctg caccagt
//
LOCUS       DAWDRC010000017        16508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1901_length_16508_cov_2.686866, whole genome shotgun sequence.
ACCESSION   DAWDRC010000017 DAWDRC010000000
VERSION     DAWDRC010000017.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 16508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1901_length_16508_cov_2.686866"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3820..3829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4854..4917
                     /estimated_length=64
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6544..6553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13926..13935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4128 a         4305 c         4041 g         3940 t
ORIGIN      
        1 agggagttat tccggcctga caagaggaaa tttaaaataa ttttctgacc gcgcattttt
       61 tcagcaaaag ccccgttaat gtgccataaa acaagcaatt tgccgttttt gcaaccgtaa
      121 tcacacttcc tggtggcata ctaagaagga ataaaaagaa aaaggaagat tagtaatgcc
      181 tttaagcgca caacagttag ctgcacagaa aaacctttcg tatgttctgg ctgagaagct
      241 ggcgcagcgg atcttaaaag gtgaatatga acccggcacc attttgcctg gtgagattga
      301 gctgggcgag caatttggag tgagtcgtac agcggtacgc gaagcggtca aaacgttaac
      361 ggcaaaaggg atggttttac cgcgaccgcg aattggtacc cgagtcatgc cacaatcgaa
      421 ctggaatttt cttgatcagg aattgcttac ctggtggatg acagaagaga actttcatca
      481 ggtcatcgat cactttctgg ttatgcgtat ctgtctggag ccgcaagctt gcctactggc
      541 agcaaaggtt ggcaccgcgg aacagaaggc gcatctcaat acgttaatgg ccgaaatggc
      601 ggcattaaaa gagaactttc gccgtgaacg ctggatcgaa gtcgatatgg cctggcatga
      661 gcatatctat gaaatgagcg ccaatccgtt tttgacctca ttcgcctcgc tattccattc
      721 ggtttatcac acttacttca cgtcaattac cagcgacaca gtgataaagc tggatctgca
      781 ccaggcgatt gtcgatgcga ttatccaaag cgatggcgac gcggcattta aagcttgtca
      841 ggcgctgcta cgctcaccag ataagtgata acccgataac aggacattga atgagcgata
      901 aaaagaagcg cagtatggcg ggtttgccgt ggatcgcggc gatggccttc ttcatgcagg
      961 cacttgatgc cactattctg aataccgcct tacccgcaat cgctcatagc cttaatcgtt
     1021 ctcctctcgc gatgcaatca gccatcatca gttatacgct gacggtggcg atgcttattc
     1081 cggtaagcgg atggctagcc gatcgcttcg gtacacgtcg catttttacc cttgccgtga
     1141 gtctgttcac gttgggttct ctggcctgcg cactttccaa ctcgctacca cagctggttg
     1201 tcttccgggt tattcagggg ataggcggcg caatgatgat gcctgttgct cggctggcct
     1261 tactgcgcgc ttatcctcgt aatgaacttc ttcctgtatt gaattttgtc gccatgccgg
     1321 gtctggtggg gccaatttta ggccccgttc ttggcggcgt gctggtcacc tgggcaacct
     1381 ggcactggat atttttaatc aatatcccca taggtattgc gggccttctt tacgcgcgca
     1441 aacatatgcc caatttcacc accgcacgac gcagattcga tatcactggc tttttgctgt
     1501 ttggcctcag ccttgttctc ttctcaagcg gaatagagct attcggggaa aagattgtcg
     1561 ccagctggat tgccttgacg gtaattgtca ccagcatcgg gttactgctt ctctatattc
     1621 tccatgcgcg acgcacgcca aacccattaa tttcattaga tttatttaaa acccgcactt
     1681 tctcgatcgg tatcgtaggc aatattgcaa cccgtctggg gaccggctgt gtaccgttcc
     1741 ttatgccatt gatgttacag gtaggatttg ggtatcaggc gtttattgct ggctgtatga
     1801 tggcaccgac agcgttaggt tccattattg caaaatcgat ggttacccaa gtcttacgtc
     1861 gtctgggcta tcgccatacg ttagtgggga tcacggtgat tatagggcta atgatcgctc
     1921 agttctcttt gcaatcacca gcaatggcga tatggatgct gatcttgccg ttgtttatat
     1981 tagggatggc tatgtcgaca caatttaccg cgatgaatac catcacactt gccgatctga
     2041 ccgatgacaa cgccagcagc ggtaacagtg ttctggcggt cacgcagcaa ctgtcgatca
     2101 gtttaggcgt tgctgtaagt gcggcagtcc ttcgcgttta cgaagggatg gaaggcacaa
     2161 cgacagtcga acaattccac tatacgttta tcacgatggg cattattact gttgcttcag
     2221 cagcaatgtt catgctactg aaaacaaccg atggtaataa tttgatcaaa agacagcgta
     2281 aatctaagcc gaaccacgtt ccatcagaat cggagtaagt tgtaatcgtt gttgctgtag
     2341 ggtcggctgg gttatccgat ggatgagtac atcaatcgcc agctccccca gttcatcttt
     2401 cggttggtgt atagttgtta atggtggcgt cataaagctt gccagttcga tatcgtcata
     2461 gccaatcacc gcgatatcct gcggaacctg taactctgcc tgatataacg cctggtaaac
     2521 gccaacagcc atagcgtcat ttccggtaaa gacggcctga ggacgcagcg gatgtgataa
     2581 cagttggcgc atagcgtcaa acccgccgtt aaattcaaaa tcaccagtga cttcatagcc
     2641 atcaggaatg ttgagacccg cacgtttcat cgcagcccga taaccttcca accgcaggcg
     2701 cgccggagtt ttatccagcg gaccggtaat acaggcaata cgggtatgac ctttatcgat
     2761 cagatattgc gttgctaagt ctccgcccag caacgagtta tcctgaataa gatcgctgtc
     2821 gccatcgaac ggagcccagt ccatcatcac ggtaggcact gtcggataac gttgcatgat
     2881 ttcacgcgaa ggctgatgcg tttcggtgca cagtaacagc aagccatcaa cgcgtttttg
     2941 catcagcgtt tccagattac gattcatccg ctgttcatcg ccttcggtat tgcaaaggac
     3001 aagactataa ccgcgttcga agcagctgcg ttcaacgcca cgcaccagtt ctgaatagaa
     3061 aggattggta ctggcagtga tcaacatgcc aatggtatgt gtttgattga gtttgaggct
     3121 acgcgccaga gctgatggcg cgtaattgag ttctttaatc gccgcttcaa ctttggctgt
     3181 aatcgcttca ctgacgaagc gatctttatt gataacgtga gaaactgttg aggtagaaac
     3241 gcccgccagg cgggcaacat ctttcattgt agccaagcgt cacctctgcc tgtctaaaaa
     3301 tgcgtcgatc tcttcacgcc acggtacgga aggttgtgcg cctttacgtg ttacggcaat
     3361 cgcagcggct gcatgggcaa aacgaatcgc ctctggcaat gttttttctt ccagcaacgc
     3421 cgtgattaac gcgccgttaa aggtatctcc ggcagcaatg gtatcgacag cctgcacccg
     3481 gaatccagga acgcgctgac cttcaccatt cacgctagcc catacaccac gacttcctaa
     3541 agtaatcagt acagtacgga tacctttttc atgcagtacc tgcgccgcct tcgctgcatc
     3601 ttcatcattt tcaacacgaa taccggtgag cttttctgct tccgtttcgt ttggcgtaat
     3661 aatgtccacc agcgccagca gttcgtcagg aagttcgcga gccggagccg ggttaagcgc
     3721 aacgatagtc ttattttgat gggcgatttt cgccgctgcc atcacacttt cgagtggtga
     3781 ttccagctgc attaataatg ctgacgcgtt ggcaatacgn nnnnnnnnnc gccacacctg
     3841 ttgattcgcc tttgatcacg ctgaccggag aaatatcgat gttatcagtg gcgagctgct
     3901 ggcgaacgct ctcaccaatg ctgtcatcgc cagtgcaggc aataaacgcg atattcgcac
     3961 cgctacgccc agcagccacc gcctgattcg cgcctttgcc gccaaatgca acctgatagt
     4021 ggttaccggt tacggtttcg cctggagtag gaaaagattg aagattaaga atgtggtcag
     4081 cattaatgct gccaagaaca acgaggctgc ctgcgttttg catattcggg atgtccataa
     4141 aatgcgccac cgtgttaggg tggcgcgtgc cctgcttttc tttatattac acgttcatct
     4201 gtcgatgacg tatttatgtc accatcaggt catacaacct gattaaaact actgcttaac
     4261 aaccagtttc agatcaaccg gatacttagc ctgaactttc tcgcctttca gcactttatc
     4321 tgcggtttcg acgcctttcg cgccaatctg atcgggtagc tgagcgatag tcgctgctag
     4381 tttgccatca ttcaccgctt tttcgccatc cggtgtaccg tcaaatccga cgaccatcac
     4441 atccgattta ccggcagttt gcagtgcgcg cagcgcgcca agcgccattt catcattctg
     4501 cgcgaataca gcctgaacat ccggatgagc ggtcaacagg ttctgcatta cgttcaaacc
     4561 tttggtgcga tcaaaatctg caggctggct ggcaagaaca ttaaacttgt gagcagcaac
     4621 ggcctgctgg aaaccttcgc cacgttcacg ggctgcggat gtaccggcaa tgccttgcag
     4681 ctcgataact ttggcacctt cacccacttt cttcgcgatg taatcaccgg cgattttgcc
     4741 gcccagtacg ttatcagatg caatgtggct caccacttca cctttcgttg cctggcggtc
     4801 aagagtgata accgggatgt tcgcctggtt agccatcttc acagcattac ctannnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntta
     4921 agcgtggaga ccaccagcgc gatggtgtct tttgccatcg cattcgcact gacggtggcg
     4981 cttagcgcaa cagcggaaac cagggtagcc agttttttca tgttcatatt caagatgtcc
     5041 tgtagtcgtt attactgctt tttgttgtct accagcaccg ccagcaaaat caccaccgct
     5101 ttgacgatca tctggtaata ggaggaaaca cctaacaaat tcaatccatt attaaggaag
     5161 ccaagaatta atgcgccgat caacgtccca acaatgcgac cttttccgcc cgccagactc
     5221 gtaccgccca gaaccaccgc agcaatagca tccagctcat agccagtccc cgccgtgggt
     5281 tgtgcggagg agagacgcgc cacttcaatg atcccggcca gcgatgccag cagaccacaa
     5341 agagaataga cgatgatttt gattttattg acgttgatac cagaaagacg cgttgccgct
     5401 tcgttgccgc ccagcgcgta gatgtaacgc cccagacgcg tgtgatgcag catgtaccag
     5461 gccgcgagga agacaatccc catgatccag actggcgtcg gtacgcccag cggacgacca
     5521 ataccaaacc agccaaacag atcggcgttc tcagtaaagc cggtattcac tgggctacca
     5581 ttggtataaa ccatggtcac gccgcgcagt aaaagcatca taaccagcgt agcgataaac
     5641 gcctggacgc gacctttcgc tacaatcacc ccagttacag caccaattgc ggcacctaac
     5701 gcgagagcgg cagcgacagc caccagcgca ttgacttcaa tgccgacgat agatgcagca
     5761 actgcgccgg tcagcgccaa cagagaacct accgacaagt cgatacccga cgtcaggatc
     5821 accagcgtca tcccgaccgc cataatggcg ttcaccgagg tttgctgaag aatattgaat
     5881 aagttattga tggtgaaaaa gttcgggctt aacgtcgaga caatcgcgat cagcaccagc
     5941 agagcgataa gcgatttctg ctccatcagc cacgctttcg tgaaataacg gcgaccagag
     6001 acagtctggg ttgtcatttt tttactcctg attcacgcga ttaagcttgc ccacagcggc
     6061 agccattaac acttcctggg tggcctgctc acgagtaaat tccccgctga gatgcccttc
     6121 atgcatgacg atgatgcgat cgctcatgcc taatacttct ggcatctccg atgacaccag
     6181 aatgatgctc aagccatcgg ctttgaactg gttaatcagt tgatagatct cttttttcgc
     6241 gccgacatct acgccacggg taggctcatc gaggatcaac actttagggc gtgtcatcag
     6301 accacgggca atcgctactt tttgctgatt gccaccggaa agcagaccaa ttgcctgttc
     6361 catcgatggc gttttcacat taaacagacg aatgaaatca ctcacagcct gctgttcatc
     6421 ggcatgcttc aaactgccgc cagcgcggct gaagtagcgc agcgcggtca gcgacatgtt
     6481 ctcttttact gacatgccca gcactaaacc gtcacgttta cggtcttcgg agatataaac
     6541 aatnnnnnnn nnnataaaca atgccgtttg ccaggccatc ctgcggcgaa cgggtaacga
     6601 cttcatgccc atccagggtg acgtaaccgc ttgtgcgcgg cagtgcaccg tagagcactt
     6661 tcatcagttc ggtacgccca gcgcccatca aaccagagac gccaagaatt tcgcctttgc
     6721 gtaaagtaaa agagacatcg ttaacgccag gtccgcagag attatcgact ttcaggcgga
     6781 tatctcccgg cgctttgtcc aggtgcggat attgatcttc cagcttgcgg cccaccatca
     6841 tctcaatcag agaatcttcg gtcagtgatg ccacttcgcg ctcagcaata aattgtccgt
     6901 cacgaaaaac ggtaacgtca tcgcaaatct cgaagatttc tttcatgcgg tgggagatat
     6961 agacaatacc gcggccttgc gatttcagct cgcggatgac gcggaacaag gattcggttt
     7021 cggtatcggt cagcgcatcg gtcggttcat ccataatgat gactttcgac tcaaaactca
     7081 gcactttggc gatttcaacc atttgctggt caccgatgga aagatcgccc accagcttgt
     7141 cgcttttaaa gcgcaggtta agtttagcca gcaatttatc cgcttcggca tacatggttt
     7201 tccagtcaat tttgccaaag cgattaacaa actcacgacc gaggaaaatg ttttcggcaa
     7261 tggttaactg cgggatcagg ttcagttcct ggtggataat cccaatcccg gcttcctggg
     7321 aagatttcgg cccggtaaat gtcgtttctt tccccaacca taaaagcgta ccggcatcgc
     7381 gagtatagat accagtaagc actttcatca tggtggattt acccgcgccg ttttcgccca
     7441 ccagcgccat cacgcggccc ggatagacat ttaacgctgc gcccgagagg gcttttacgc
     7501 ccgggaaggc tttatcgatg cctttaagct gaagtaatgc ttccatgacg gcctcagaac
     7561 gtcacgccag cacagagaat gatattcgca tacggagaac attctccgct gcgaattacc
     7621 gcctgacttt ctgcggtttg ttgtttgaat tgttcatgcg tggtgtaacg aatctcaatg
     7681 gtgtttccct ggtgttgttg cagctgctca aggtgagtga gcaacgtttc gtggagttgc
     7741 ggattatgtt gtttgatctc ttccgcgata atgacagcct cgacctgcat ttcatttgtg
     7801 acgacgccca gcacctgcat aaaagaaggt acaccctggg ttaatgccat atcgatacgc
     7861 gttgcacttt tggggatggg taaaccagca tcacacacca ccagcgtatc ggtatgtccc
     7921 agtcgggaga tcaccgatga aatatcagaa ttaagaacgg tgcctttttt catttttttc
     7981 tccatcagcg aaacgtttcg ctgacccgca gtttaatcct gactgagttg ggaaaaccat
     8041 cgtgacgtta cggaaatgtg atcaacctcg aaacgtttta catggtgatt aaccatgaaa
     8101 acaaaaacgc ccccttatgg agaaaggagg cgtctggcgt tagatttcga cctgagtacc
     8161 cagttcgata actctgtttg gcgggatttc aaattgatcc ggtgcccgca gcgcattacg
     8221 ttgcagcagc aagtacagct tgccgcgcag acgcaaatac cacgggcgtt tgccgaggat
     8281 caacgactca tgcgacataa agaaggaggt ttccatcatc cggcaactta atccttccag
     8341 accgcagcgg tggaaaactt cttccacgtt tggcgtttct cgccaaccat aacttgccac
     8401 cacgcgccag aaagtgggcg acagttgttc aatctgtacc cgacggacgt tatggacata
     8461 tggcgcgtct tcggtgcgca gagttaacag aatcacccgc tcatgcaata ccttgttatg
     8521 tttcaggtta tgcatcagcg caaagggaat gacgttgatt gcacgcgaca tatacaccgc
     8581 ggtcccaggc acgcgaacgg gcggtgattt ctccagcgaa gcaatcatcg cttccagaga
     8641 gttaccatgt tcatgcatcc gccgcagcaa gcggaaacgc tcgctcttcc aggtggtcat
     8701 cacgataaac atcaccgtac cgaggctcaa tggcaaccag ccgccggaga gcagtttatc
     8761 gaggttagcg gtgaacaagg gaatatcgac acaaaggaaa gcaatcagga tcagcgcaac
     8821 aaaatactta ttccagtgcc agttctgacg tgccacggta gtcgagagaa tagacgtcag
     8881 caccatggtt ccggtcaccg caatcccgta cgccgccgcc aggttgctgg agtgctcaaa
     8941 gctgacaatc acaatcacga ccgcgacata gagcatccag ttcacaaagg gaatatagat
     9001 ttgccctgac tccatttcgg aagtgtgaat aatgcgcatc ggcgacaaat atcccagacg
     9061 taccgcctga cgcgtcaatg agaagacgcc agagataacc gcctgcgagg caattaccgt
     9121 cgccagtgcg gcgatgatca gcagcgggat cagcgcccag tccggtgcca acaggaagaa
     9181 cgggttctta atcgcttccg gattctttaa caacagcgct ccctggccga agtaattaag
     9241 ggttaaggaa ggcaatacga cggtgaacca cgccagacga atagggaact taccaaagtg
     9301 ccccatatca gcatacagcg cctcgacccc cgtaatcgac agcaccactg cccctaatgc
     9361 aataaaagaa accgttttgt attcaaggaa gaaatgcacc gcccacatcg gattcagtgc
     9421 atgcagcact tccgggttag caataatgct acgtaacccc agccctgcca gaatcaaaaa
     9481 ccaggtcagc atgatcggcg caaacagctt accgaccata gccgtgccat gtttttgaat
     9541 catgaataat aatgtgagaa cgataattga gaggggaact atccaggtat ccagctgcgg
     9601 ggcgacgatt tccagacctt caatggcgga catcaccgaa atagcgggag taatgacgac
     9661 ttcaccatag aaaaagctgc cgccgattag ccccataatc accagcattg atgtggttcg
     9721 cgccgacgta ttacgcccgg caagcgacat caacgtcagg atcccccctt caccggcgtt
     9781 atctgcccgc atcacgaagg tgagatattt aatggaaacc acaaagatta gcagccagaa
     9841 gatcagcgat aaaaagccaa acacggcatc gcgttcaacg ccaaaaccaa actggccgga
     9901 caaacattca cgaagtgtat ataacgggct ggtaccaata tcgccgtaga caactccaat
     9961 cgccgcgagg gtaatcgcgg gcaatgattg cttattatca gtgctcatag actagtcttt
    10021 cgttgaaata tgaaatgtgt gcttagtccc ttggcccaca aaaaagcgca cagtatgcac
    10081 gattaacggc aaaatcgtac tcctaaatgc ggccacatta acctggctca aagaaaaata
    10141 cccctccttt gagactattt tagccaccag caagaaacgt ctatactcgc aatttacgca
    10201 gaacttttga cgaaaggacg ccacttcatt atggctcacc ctcatttatt agcggaaaga
    10261 atttcccgcc tgagcagttc gttggaaaag gggctttatg aacgtagcca cgccatccgc
    10321 ttgtgtttat tagcggcatt aagtggtgaa agtgtgttcc tccttggccc accaggtatt
    10381 gccaaaagtt tgatcgcccg gcgcttaaaa ttcgcctttc agaatgcccg cgcgtttgaa
    10441 tatctgatga cccgcttctc cacgccggaa gaagtttttg gtcccctttc tattcaggcg
    10501 ctaaaagatg aagggcgcta tgaacgttta accagcggtt acctgccgga agctgaaatc
    10561 gtctttctgg atgagatctg gaaagcgggt ccggcaattc ttaataccct gctcaccgcc
    10621 attaacgagc gccagttccg caacggcgca ctcgtagaaa aaatcccgat gcgtctgctg
    10681 gtggcggcct ccaacgagct gccggaagca gacagcagtc tggaagcgtt atatgaccgc
    10741 atgctgattc gtctgtggtt agataaagtg caggataaag cgaatttccg ctccatgctg
    10801 accagtcaac aggatgaaaa tgacaatccg gttcctgcct ccatgcagat cacagatgaa
    10861 gaatatgaac gctggcagaa agagattggt gaaattacgc tgcccgatca tgtatttgag
    10921 ctgattttta tgctgcgcca gcaactggat aaattaccgg atgcgcctta tgtctcggat
    10981 cgtcgctgga aaaaagcgat ccgcttattg caggccagcg ccttttttag cggtcgcagt
    11041 gctgtcgccc cggttgatct cattttgctg aaagattgcc tgtggtatga cgcgcaaagc
    11101 ctgaatttga tacaacaaca aattgatgta ttgatgaccg gtcacgcctg gcaacagcaa
    11161 gggatgttga cccgcctggg cgcgattgtg caacgtcacc tgcaacttca gcagcaacaa
    11221 agcgataaaa cagccttaac ggtaattcgt ctgggcggca tttttagccg tcgtcagcag
    11281 tatcaactcc ctgttaacgt tactgcttcc actctgactc tgctgctgca aaaaccgtta
    11341 aaactgcatg atatggaagt ggttcatatc tcctttgagc gtagcgcgct ggaacagtgg
    11401 ctgagtaaag gtggtgagat tcgcggcaaa ctgaacggta tcggctttgc ccagaaactg
    11461 aatctggaag tcgatagcgc ccaacatctg gttgtacgcg atgtgagttt acaaggcagt
    11521 acgctagcac ttcccggttc atcggctgaa ggtctgccca gcgaaataaa acaacaactg
    11581 gaagagcttg aaagcgactg gcgcaagcaa cacgctttat tcagcgaaca gcaaaaatgt
    11641 ctgtttatcc ctggcgactg gttaggtcgc attgaagcca gcctacagga tgtcggcgca
    11701 cagattcgcc aggcacaaca atgctaacgc tggatacgct taatgtgatg ctggccgtca
    11761 gcgaagaggg attgatcgaa gagatgatca tcgcgctgct ggcctcaccg cagctggcag
    11821 tcttctttga aaaattccca cgactgaagg cggcaatcac tgatgatgtt ccccgctggc
    11881 gtgaggcgct gcgcagtcgg cttaaagatg cccgagtccc gccggaactc accgaagagg
    11941 tgatgtgcta tcagcaaagt cagctcctct ccacgccgca atttattgtg cagctaccac
    12001 agatcctgga cttactgcat cgtctgaatt ccccatgggc agaacaagcc cgacagttgg
    12061 ttgatgctaa cagcacgatc acttcagcgt tacacacgct ttttctccag cgctggcgtt
    12121 taagcctgat cgtgcaagca acgacgttaa atcagcagct attagaagaa gaacgcgaac
    12181 aactgttgag tgaagttcag gaacgcatga cgctgagcgg gcaacttgaa ccgattctcg
    12241 cagataacaa taccgcagct ggtcgtctgt gggatatgag cgccggtcag cttaaacgtg
    12301 gcgactatca gttgattgtg aaatacggtg aatttcttaa cgaacagccg gaactgaaac
    12361 gtctggcaga acagctgggg cgttcccggg aagccaaatc aataccgcgc aacgatgcgc
    12421 agatggaaac cttccgcatc atggtgcgcg aaccggcgac agttcctgag caggttgatg
    12481 gtctgcagca aagcgatgat attttacgtc tcttgccgcc agaactggcg acactaggga
    12541 taacggaact ggagtatgag ttttaccgtc ggctggtgga aaaacagttg ctcacctatc
    12601 gcctgcatgg tgagtcgtgg cgtgaaaaag tgatcgaacg cccggtggtg cataaagatt
    12661 acgacgaaca gccgcgcgga ccgtttattg tctgcgtgga tacttccggc tcaatgggcg
    12721 gctttaatga acagtgtgcg aaagcgttct gcctggcctt gatgcgcata gccctcgctg
    12781 aaaaccggcg ctgctatatt atgctatttt ccaccgagat cgtccgttat gagctttcag
    12841 gcccacaagg catcgaacaa gcaatccgtt ttttaagcca gcagtttcgt ggcggcaccg
    12901 atcttgccag ttgttttcgc gccattatgg aacgcttgca aagccgggaa tggtttgatg
    12961 ccgatgcggt ggtgatttct gattttatcg cccagcggtt gcctgacgac gtgacgagca
    13021 aagtgaaaga gttgcagcgg gtacatcagc atcgctttca tgccgtggcg atgtcggcac
    13081 atggcaaacc cggcatcatg cgcattttcg atcatatctg gcgctttgat accgggatgc
    13141 gaagccgcct gctcagacgc tggcggcgat aaattattac agcagagaag ggacgctctc
    13201 gcgaacagct gccggccata caccacactg aacctggccg atatgtggca gttgcagcag
    13261 cagcatggtc aaacgagact gcccgatacc gccgccgatg gtctgcggca tttcaccgcg
    13321 cagcagcgcc tgatgccact ccagctgcag gcgatcttcg tcaccggtca gcgccagctg
    13381 atgcttcagc gtgtcggcat ccacgcggat ccccatggaa gaaagctcaa acgcatcttc
    13441 cagtaccggg ttccatacca gaatatcgcc gttcagaccc gcatggccca gctctgacgg
    13501 ggtgctccag tcatcataat ccggtgcgcg cacgtcgtga cgatgaccat cactcaactt
    13561 gccgccaatc ccgacgagga ataccgcgcc aagatctttc gcaatcgccc gctcacgccc
    13621 tttggcatcg agatccggat aacgagacag taactcctgg ctgtgtacga agtggatttg
    13681 atccggcaga aacggtgcca ggccaaactc ttcgctaacc gcagcttcgg ttgctttaat
    13741 tcccgcccag atcgcctcta ccgtgctttt cagagtcgag aattgacgct cgccgtcgcc
    13801 cattacgcgt tcccagtccc actggtctac atagaccgag tgcaacgggg aaagacggtc
    13861 ttcatcgggg cgaagggctt tcatgtgcgt gtacagccct tcgcccgcgc tgaagtcgtg
    13921 ttgccnnnnn nnnnntttcc acttcgccag tgaatgaacc acttcaaact gggcatcagg
    13981 cagagctttc acttttacct gcaccgcttt ttcacagccc gacaagttat cctgcgtgcc
    14041 atcccccaca cggctaagaa tcggcgcctg gacttcgatc agccccagac gttcttccag
    14101 ttgacgagaa aagtgagatt tcacgaagct aatttgacgt tgtttggcaa tgtaagcggt
    14161 tttcattttt tatactcctg cgtcctgttg cttatgatta agcaacaaaa accgacacac
    14221 atgcaataat cattcaataa aaagcctcac gtacttttga ttcattaaaa aagaaggcta
    14281 aaatagaatg aatcatcaat ccgcataaga aaatcctatg gaaaattatc tgatcgacaa
    14341 tctggaccgt ggcatcctgg aagcattaat gggcaatgcg cgcaccgctt acgccgaact
    14401 ggcgaaacaa tttggcgtca gtccggggac gattcacgtt cgagtagaga aaatgaagca
    14461 ggcggggatc attaccgggg cgcgtattga tgtcagcccg aagcagcttg gttacgtagg
    14521 ctgctttatc ggcattatat taaagagcgc caaagactat ccttccgcgc tggcaaagct
    14581 ggaaagcctc gatgaagtca ctgaagccta ttacacaacc ggccactaca gcatctttat
    14641 aaaagtgatg tgccgttcga tcgatgctct ccagcatgta cttatcaaca agatccaaac
    14701 aattgatgaa attcagtcca ccgagacact gatcgtcctg cagaacccga tcatgcgtac
    14761 cattaagccc tgatcggctt ttttaatccc atacttttcc acaggtagat cccaacgctt
    14821 tcacagcgta caatacgcca ctcttaataa aggtggcggt ttatggcaga tatcactctt
    14881 atcagcggca gcaccctcgg cggtgccgaa tatgtagcag aacacctggc tgaaaagctg
    14941 gaagaggcgg gttttaccac cgaaacgctg cacggtccgc tgttggaaga tttatctgcc
    15001 tcggggatct ggctggttat cagctccacc cacggtgccg gagatattcc ggacaacctt
    15061 tctcctttct atgaagcatt gcaggaacag aagcccgatc tttctgcggt ccgctttggc
    15121 gcaatcggta ttggtagtcg tgaatatgac accttttgtg gggctattga taaactcgag
    15181 gctgaactca aaaattccgg tgcaaaacag acgggcgaaa cactgaagat caacattctt
    15241 gatcacgaca ttccggaaga tccggcagaa gaatggctgg gatcgtggat taatttactc
    15301 aaataagtat acagatcgtg cgatctactg tggataactc tgtcaggaag cttggatcaa
    15361 acggtagtta tccaaagaac aacggttgtt cagtttttga gttgtgtata acccctcatt
    15421 ctgatcccag cttatacggt ccaggatcac cgatcattca cagttaatga tcctttccag
    15481 gttgttgatc ttaaaagccg gatccttgtt atccacaggg cagtgcgatc ctaataagag
    15541 atcacaatag aacagatctc taaataaata gatcttcttt ttaataccca ggatcccagg
    15601 tctttctcaa gccgacaaag ttgagtagaa tccacggccc gggcttcaat ccattttcat
    15661 accgcgttat gcgaggcaat caccatgttt tatccggatc cttttgacgt catcatcatt
    15721 ggcgggggtc atgcaggcac cgaggccgcg atggccgcgg cgcgtatggg tcaacagact
    15781 ctgcttttga cacacaatat cgacactctg gggcagatga gctgcaaccc ggcgatcggc
    15841 ggtattggga agggacatct ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa
    15901 gcgatcgatc aggcaggtat ccagtttagg atactaaacg caagcaaggg accggcggtt
    15961 cgcgctaccc gagctcaagc ggatcgtgtg ctctaccgtc aggcggtacg tacggcgctg
    16021 gagaaccaac cgaacctgat gatcttccag caggcggttg aagatcttat tgtcgaaaac
    16081 gatcgcgtgg tcggtgccgt tacccaaatg ggactgaagt tccgtgccaa agctgtcgtg
    16141 ctcaccgttg ggacgttcct cgacggtaaa attcatatcg gtctggataa ttacagcggt
    16201 ggccgtgctg gtgatccgcc gtccattccg ctctctcgcc gtttgcgtga actgccgctg
    16261 cgcgttggtc gtctgaaaac cgggacaccg ccgcgtattg atgcacgtac tatcgacttc
    16321 agcgtgctgg cgcaacagca tggcgataac ccaatgccgg tattctcgtt tatgggtaat
    16381 gcgtcccagc atccacagca ggtgccgtgt tatatcaccc ataccaacga gaaaacccat
    16441 gatgtgatcc gcagtaacct cgatcgtagc ccaatgtatg caggggtgat cgaaggtgtc
    16501 ggcccacg
//
LOCUS       DAWDRC010000018        15963 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1964_length_15963_cov_2.892947, whole genome shotgun sequence.
ACCESSION   DAWDRC010000018 DAWDRC010000000
VERSION     DAWDRC010000018.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15963)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1964_length_15963_cov_2.892947"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4267..4276
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6868..6877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8163..8172
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    13533..13542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4077 a         4064 c         3778 g         4004 t
ORIGIN      
        1 gtatattacg ctttcctctt tcagagtcaa ccctgaattt caggattttt ctctttgttg
       61 ttacgactat cttgtgaagt gtttcacgtt gtcgtctcaa cggaggcgca ttatagggat
      121 cccaattttt tgcacaagta cttttttgat ctttttttct gtttgttgtt ttttcaccct
      181 ttttgctgca ttcgcacaca aaacggtgct tttttgcata ctaaaagact tgcacaaggc
      241 caataatgta cccaaagtca ttagtaaatc atttattgtt gaggtgagta tgtctgatgt
      301 tttacgccca taccgcgatc tttttccaca aatcggtcag cgcgtaatga tcgacgatag
      361 cagcgtcgtg attggtgacg ttcgtctggc tgatgatgtg gggatctggc cgctcgttgt
      421 gattcgtgga gatgtacatt atgtacagat cggagcacgc accaatatcc aggatggcag
      481 tatgttgcat gtcactcata aatcctcgta caacccagct ggcaacccat taaccattgg
      541 cgaagatgtc actgttggtc acaaggtgat gctccacggc tgcaccattg gcaatcgagt
      601 tttggttggg atgggctcaa ttttacttga tggcgcaata gtagaagatg atgtgatgat
      661 tggtgcaggt agcctggtcc cacaaaataa acggctggag agcggatatc tgtatctcgg
      721 tagccccgtc aaacagatcc gcccgttaag tgatgaagag aaggctgggt tacgctattc
      781 cgcaaataac tacgtgaaat ggaaggacga atatatggat cagggtaacc acacccaacc
      841 ttgatcatct tcactttgtt cctgaattaa gttttccgct tcttcttcca ggtcccagcg
      901 atgctgacga aaactcgcta accactgttc tggcgtatct ccagtaaagc gatacgccag
      961 actctcgcca gagatcgcac atgtcagttg cataccattc acgagagcgg gaaaacatac
     1021 acatttttta ctctcgttcc actcttccct atccggaaac tggatggcct gattcacgct
     1081 gacaactcct gttgcaatag tttgatgacc ggttctacat caggcagaac gccatgccaa
     1141 agaaggaagg catgagccgc ttgtgccacc agcattccta aaccatcagc agtacgcttt
     1201 gagcctcgct gctcacacca tgccagaaaa ggagtttttc ctttctgata gaacatgtca
     1261 tagcaataaa tgcctggata aatgagcgat ggcgggatcg ccggaatatc accactgatg
     1321 ccactggatg ttgcattaat aatgagatca aactcatgac cttccagttc gtccataccc
     1381 aacgcgtgaa tactgccagt gtgcgcaaac aatttagcca actcttccgc gcgggatacc
     1441 gtccgattag tgattgtcac cgcacagtcc agggaaagga gtggcagtag tacgccgcga
     1501 gatgctccac cagcgccaat gagcagaata cgtaaaccag gacggataaa agacagacgt
     1561 tccagatcgc ttaacaaacc tataccatcg gtattgtcac cccgcaagcg tccatcttct
     1621 aaccgcttga gggtattaac agcaccagcc aacgctgccc gttcagtaag ctcatccgct
     1681 ctggcaaaag cctcttcttt aaaaggtacc gtcacattcg cacctttacc accagcacta
     1741 aagaaagcgt tcagagtgtt gatgaaatca ttgatgggtg ccaacacgcg cccataggga
     1801 tgttcaatat tcagttgctg agcaaattgc tgatgaatga atggcgattt gctgtgggct
     1861 atcggattac caaaaacagc ataggtttcc attatgttac ccctgtcgaa acagttcacc
     1921 cgtcagggca tcgcggattt ctgaaggatt taaacgcccc cccgtttcac caggcacaac
     1981 cgggaacgcc gcgccaaatt gtgcgcgaac ttcgtctact gttcgacaag gtggcaatcc
     2041 actcaagttg gcactggtag aaaccagcgg tttaccataa gcctggcaca aagcaaccac
     2101 caacggatgg tcggtgactc gtacagcaag cgaatcaaag cggcccgtca accagcgcgg
     2161 tgttgtcgca ggcgcgggaa aaacaaaggt gacaggacct ggccagcgag aaaatatagt
     2221 ttcacgctgc gcgtcagtca gcatggtgtc atcaatatag ggtttaagct gctcgtaatt
     2281 tgcggcgatt agaatcagcc ccttatcaac cggacgctgt tttaactcca acagtcgcat
     2341 cactgctgtt tcgctatcag gatcgcaccc gacaccgaaa acggcttccg ttggataggc
     2401 gatgacacgt tcttcattga ggacatctat cgcagctgcg atagcgtctc cttgcaggtt
     2461 attattcacg ttattattcc gccgaaaccg gctttccaca ttgtttactg gcacaaaagt
     2521 gttttacacc ctgcgcggtt ttcttttcga tgagtagcgg ataatgacac tcagggcatt
     2581 ctccagctat gggtttgaag ttaatggcaa attgacactc cgggtagcga tcacaggagt
     2641 gaaacgtttt gccataacgg gagcggcgct ggaccagatg gcccgtccgg cattgggggc
     2701 atgtaattgc tgtttcgtcc ggtttatcga taagttcggt atgttcgcat tcagggtagt
     2761 tactgcaacc aataaacata ccaaagcgtc cctggcgtaa taccagattt gcgccacatg
     2821 cagggcaaac ctgcccctcc agaactttga caatatgtcc atccgctgaa gatttcagag
     2881 gacggacgta gtcacacgcc ggatactgtg agcatccaag aaacggaccg tgtttcccgg
     2941 atcgaataac cagttcagcc ccgcactttg ggcaggactc attattacgc accgtgaaca
     3001 gtgctgattt cgccataaca acttatgctg aattaaagaa atgattaatg cagcatacct
     3061 tcattcactt caaagagtaa ttcttccatt tgctggtacg cattttcgca gcccggaata
     3121 ttgaacaaca ccatcaggat tacccatttc aggtcttcca ggtcgaactc tgcggtatcc
     3181 agcgcgagca ctcgctctat caccatttca cgggtttcaa ggttaagcac ctgaatttgc
     3241 tcaaggaaaa gcagaaaacc acgacagctg gcatccagcc tttcacactc tccaggggta
     3301 taaatacgca tggagagtgg atcagaggcc agttgcatcg gttctgccag tccttcctga
     3361 taatccgcaa gcttttccag ccaaagcagg gcattgtaga tatcttctcg atcaaatcct
     3421 gcgtcggtaa gatcctgttc aagtttgtct tgatccacac gcaactcagc ttctgtgtga
     3481 atataggttt caaacaaata cattagtacg tcgaacatgg catgccctcc tcaatcggac
     3541 atagccgccg ggtacagctg cgatccatcc tgctaactcc agttcgagta gttgagtaac
     3601 tacctctggc acaggttggc cggcacgttc agcgacgacg tcaacaggtg ttacctcatc
     3661 tcctacgtta gccaggagct caggaaatgg caatgccacg tcttcctgat ctggtgaata
     3721 aaatgaattt tcaggggcgt ctggcaacca gtgcaatcca aattgcaagt tttccagaat
     3781 ttcttccggt tccgtcacaa gaatcgcacc ttgttttatt aaccagtgag gcccttcgct
     3841 tcccggattc cctattggac ctggcaaagc aaaaacttca cgcccctgct caagcgcaca
     3901 acgtgctgtc accagcgaac cactgcgcaa agccgcttcc accaccagta cacctttact
     3961 taggccactg ataatgcgat ttcttcgtgg gaaattgtaa gcaaggggtg gaacatcgag
     4021 gggaaattcc gagacgagag caccaccttg ctcaagtaga ctggcagcca gtggagcatg
     4081 gcggcgggga tgaatggtat taagtccatt ccccaataca gcaatgctaa cgccatttac
     4141 ctgtaaggcc gctttatgcg ccacaccatc gattccacgc gccagtccac tcgtaattgt
     4201 cactccacgc gtcgccagag tttcgcaaaa taatcgtctt tgcgaaactc tggcgacgcg
     4261 tggagtnnnn nnnnnnaaat gaaagaaagc gttgtgcctg ccgcaatgta agccctgttt
     4321 gctgcaatac aaccgcatca atatgcgact gttttgccag ccagtgagct atacggacca
     4381 tatcatcgcc atacaagctg ctgatgctca ttaaacgcag ccaaatttct gtatcgacca
     4441 tccttatctc cctgccataa gcagccttag caatctttgc gattggtcag tgatgctgtc
     4501 aatcagaggg ggatttgtct agaatagaag aaataatctt tctaactcct gaacacatct
     4561 ctggagattt atgtcagttt tgcaagtgtt acatattccg gacgagcggc ttcgcaaagt
     4621 tgctaaaccg gtagaagaag tgaatgcaga aattcagcgt atcgtcgatg atatgttcga
     4681 gacgatgtac gcagaagaag gtattggcct ggcggcaacc caggttgata tccatcaacg
     4741 tatcattgta attgatgttt cggaaaaccg tgacgaacgg ctggtgttaa tcaatccgga
     4801 gcttttagaa aaaagcggcg aaacaggcat tgaagaaggt tgcctgtcga tccctgaaca
     4861 acgtgcttta gtgccacgcg cagagaaagt taaaattcgc gccctggacc gcgacggtaa
     4921 accatttgaa ctggaagcag acggtctgtt agccatctgt attcagcatg agatggatca
     4981 cctggtcggc aaactgttta tggattatct gtcaccgctg aaacaacaac gtattcgtca
     5041 gaaagttgaa aaactggatc gtctgaaagc ccgggcttaa ggataagaac taacgtgtca
     5101 gaatcactac gtattatttt tgcgggtaca cctgactttg cagcgcgtca tctcgacgcg
     5161 ctgttgtctt ctggtcataa catcgttggc gtgtttaccc agccagaccg accggcagga
     5221 cgcggtaaaa aactgatgcc cagcccggtt aaagttctgg ctgaggacaa aggtctgccc
     5281 gtttttcaac ctgtttccct gcgtccacaa gaaaaccagc aactggtcgc cgatctgcag
     5341 gctgatgtta tggtcgtcgt cgcctatggt ttaattctgc ctaaagctgt gctggagatg
     5401 ccgcgtcttg gctgtatcaa cgttcatggt tcactgctgc cacgctggcg cggtgctgca
     5461 ccaatccaac gctcactatg ggcgggtgat gcagaaactg gtgtgaccat tatgcaaatg
     5521 gatgttggtt tagacaccgg tgacatgctc tataagctct cctgcccgat tactgcagaa
     5581 gataccagtg gtacgctgta cgacaaactg gcagagcttg gcccacaagg gcttattact
     5641 acgttgaaac aactggcaga cggcacggcg aaaccagaag ttcaggacga aactcttgtc
     5701 acttacgccg aaaagttgag taaagaagaa gcgcgtattg actggtcact ttcggcagca
     5761 cagcttgaac gttgcattcg cgctttcaat ccatggccaa tgagctggct ggaaattgaa
     5821 gggcagccgg ttaaagtctg gaaagcatct gtcatcgata cgacaacaaa ggctgcgcca
     5881 ggaacaatcc ttgaagccaa taaacaaggc attcaggtag cgacaggtga tggcatcctg
     5941 aatctgctct caatgcaacc tgcaggtaag aaagcgatga gcgtgcaaga cctcctgaat
     6001 tctcgtcggg aatggtttgt accgggcaac cgtctggcct gatagtccac tcttctaagc
     6061 ccggtcttac cgggcatctt tatatttata cttatgaaaa aacaacgtaa tttacgtagc
     6121 atggcggccc aggccattga acaagtcgtt gagcaagggc aatcactgag caacattctg
     6181 ccaccgctcc agcaaaaagt ttccgataaa gataaagctc ttctccagga gttatgcttt
     6241 ggcgtactgc gtacgctctc acagttagac tggctgatta ataagctaat ggcccgtccg
     6301 atgacaggca aacagcgtac tgttcattac ctgattatgg ttggtttgta tcaactgctt
     6361 tatacccgca ttccacctca tgccgcgctg gctgaaacgg ttgaaggcgc agttgcaatt
     6421 aagcgcccgc aacttaaggg attgattaac ggtgtattac gtcagttcca gcgtcagcaa
     6481 gatgagttat tagccgagtt taatgccagt gatgcacgtt atctgcatcc ttcctggttg
     6541 ctgaagcgtc tgcaaaaggc gtatcccgag cagtggcaat ccatcgtcga agccaataac
     6601 cagcgtccgc caatgtggct gcgcgttaat cgtacgcatc attcccgcga cagctggctt
     6661 gcattgctgg atgaagcagg aatgaaaggt ttcccgcatg cggattaccc tgatgctgta
     6721 cagctggaaa cacctgcacc tgttcatgcg ctacccggtt ttgaagaagg atgggttacc
     6781 gttcaggatg cttcagcaca aggttgcatg acctggcttg ctccacaaaa tggtgaacac
     6841 attctggatc tttgcgccgc cccaggcnnn nnnnnnnacg catattcttg aggttgctcc
     6901 agaggcgcaa gttctggcgg tcgatattga tgagcagcgc ctctctcgcg tttacgacaa
     6961 tttaaaacgc cttggtatga aggcgaccgt gaaacaaggc gatggccgct acccttccca
     7021 atggtgtggc gagcaacagt tcgatcgcat tttattagat gcgccttgtt cagcaacggg
     7081 ggtgattcgt cgccatccgg atattaaatg gttacgtcgt gatcgcgata tcccggaact
     7141 cgcgcaattg cagtctgaaa ttctcgacgc catctggtcg catttaaaat ccggaggaac
     7201 cctggtctat gccacctgtt cggtgttacc ggaagagaat agcctgcaga ttaaagcctt
     7261 tttgcaacgt accgctgatg ccgaactttg cgaaacagga acaccagagc aaccgggtaa
     7321 acaaaatttg cccggtgccg aagagggcga cggcttcttt tacgctaagc taatcaaaaa
     7381 gtgatgagat aacgggtcgc gactgatgaa aattatcatt ctgggtgccg gccaggttgg
     7441 cggcacactg gcggaaaacc tggttggcga gaacaacgat attactgttg tcgataccaa
     7501 cggtgagcgt ctgcggacct tacaggataa atttgacctg cgagtcgtgc aggggcatgg
     7561 ctctcatcca cgcgtattgc gggaggcagg tgccgacgac gccgatatgc tggttgctgt
     7621 aaccagttca gatgaaacca atatggttgc ctgccaggta gcctactcac ttttcaacac
     7681 ccctaatcgc atcgctcgta tccgctcacc agactacgtg cgcgatgccg ataagctatt
     7741 tcattcagat gctgttccga ttgatcatct gatcgcacca gagcagttgg ttatcgataa
     7801 tatttaccga ctgattgagt atcccggcgc attgcaggtg gtgaacttcg ctgagggtaa
     7861 agtcagcctg gctgtggtta aagcctatta tggcggcccg ctgattggta atgcactttc
     7921 gaccatgcgc gaacatatgc cacatatcga tactcgtgtg gcagcaattt tccgccacga
     7981 tcgccccatt cgtccgcaag gttcgaccat tgttgaagct ggtgatgaag tgttctttat
     8041 tgccgcttca cagcatatcc gtgcggtgat gagtgaatta cagcgactgg aaaaaccgta
     8101 taagcggatc atgctggttg gtggcggtaa tatcggtgca gggctggcgc gtcgtctgga
     8161 cannnnnnnn nncgttaaac tcatcgaacg taatcagcag cgcgctgccg aactggcgga
     8221 aaagttgcag aatacgatag tcttttttgg tgatgcgtcg gatcaagaac tgctggccga
     8281 agaacatatc gatcaagttg atctgtttat tgccgtcacc aacgatgacg aggccaatat
     8341 catgtccgcc atgcttgcca aacgtatggg tgcgaaaaag gtgatggtat tgatccaacg
     8401 tcgcgcttat gtggatctgg ttcaggggag cgtgatcgat attgcgattt caccacaaca
     8461 agcaactatt tctgcgttgc ttagccatgt gcgaaaagca gatattgttg gtgtttcctc
     8521 attacgccgc ggcgtagcag aagctattga agccgttgct cacggtgatg aaagcacctc
     8581 acgcgttgtc ggcagagtca ttgacgaaat caagctaccg ccaggaacga ttattggagc
     8641 ggtggtacgt ggaaacgatg tgatgattgc caatgacaat ctgcgcattg agcaaggcga
     8701 tcacgtaatt atgttcctca cagataaaaa gtttattacc gacgtcgaaa gactcttcca
     8761 gccaagtcct ttcttcttgt aattaataag gcgtctaatg acgccttatt atttcccttt
     8821 gattattaag gattaattaa atccattcct ggcaggaaaa tggcttaaca tttgttagac
     8881 ttatggttgt cggcttcata gggagaataa catgagcatt attaaagaat ttcgcgaatt
     8941 tgcgatgcgc gggaacgtgg tggatttggc agtgggtgtc attatcggag cggcattcgg
     9001 gaagattgta tcttcactgg ttgccgatat catcatgcca cctctgggct tattaattgg
     9061 cgggatcgac tttaaacagt ttgctgtcac gctacgcgag gcccaagggg atatccctgc
     9121 tgttgtgatg cattacggtg tcttcattca aaacgtcttt gattttctga tagtggcctt
     9181 tgccatcttt atggcgatta agctaatcaa caaactgaat cggaaaaaag aagaaccagc
     9241 agcagcaaca cctgcaccaa ctaaagaaga agtattactg acagaaattc gtgatttgct
     9301 gaaagagcag aataaccgct cttaacaagc gtctgaaagc agaaggccag tggtaaaaaa
     9361 gtgatttact ttcttgccac tggcctccca gttcccccga ttgccatgtt ttccttttcg
     9421 catgtaactg cctttccctt tcttattttt ctctactcgc tgtcggaata agggatcatg
     9481 tagtaatgct tctatcgcat tatcctttat ctgcccttta gtatgctgat atcggctcat
     9541 aaaaactcca attggttatt taacggcgcg agtgtaatcc tgccagtgca aaaaatcaac
     9601 agccactctt aacgccactc gccccttgtt caagagcttc aagaatcgag caataaacac
     9661 tgctatgggc ggtcccacaa caggtatcat taaggcgttg caaggaacgc tgcattctct
     9721 gcaactcggc tatacgagct tcgacttcct gcaatctttc ctgcacaatg ccttttgact
     9781 cctgacaggt atggtgttca ggatcgatac ggatcgacag caactcgcgg atcgactcca
     9841 gactgaaacc tagttgtctg gcatggcgga taaatttcaa tcgctggaga tcgctttcgg
     9901 tatacaggcg aaacccacct tcggtgcgta cttcatgctc catcatctgc tgcttttcgt
     9961 aataacgaat cgtgtcgggt gttacttccg ccatttttgc cagctcacca atgcgataca
    10021 tacatactcc actagttatc gttgattttg ttcaacaact tgtcagcata atcgccgcgt
    10081 aaaaaatcgg tactcaatcc agcttgtcgc agcttcaatt ccagtaatga caatcgacga
    10141 ctaacctctt gatattgcgg atcatcatga cggatacctt tgagaatatc cagaagctgt
    10201 attgcttccc tcctatgctc aagttctggc ggtaagcaac cagcattctt cagcaagcga
    10261 taccccgcac gtaattccgg tggcacatga gaatcatcat ccagtatcaa tggttcgccg
    10321 ctacctacca ggttatcaaa ctcacctttc gcttgcgctt ctgctatatg acgctctgcc
    10381 cactggtcaa gtaaccacat aaatactcca ggggatgaac aaaaagagta cagctattgt
    10441 agataagtgg ggatgctacg ggtataaaaa aacccgccgg ggcgggtttt tttacgttgc
    10501 ttcagattac tctgcagcag cttctgcttt ctctgaacga tcaaccagct cgatgtaagc
    10561 catcggcgca ttgtcgcctg cacggaagcc acacttcaga atacgagtgt aaccaccggc
    10621 acggctcgcg aaacgcgggc ccagttcgtt aaacagtttt gccacgatct cgttatcacg
    10681 agtacgggcg aatgccagac gacgattagc aacgctatca gtcttggcaa gagtaatcag
    10741 cggctcaact acgcggcgca gctctttcgc tttaggcaga gtcgtcttga tgatttcatg
    10801 acgaaccagt gaacctgcca tattgcggaa catagcctgg cgatggctgc tgttgcggtt
    10861 cagttgacga ccactcttac gatggcgcat gaccttatcc ttctcagtaa aaccttaacc
    10921 tgtgatccgg ttactcgtca gcgatgcttg ccggtggcca gttttccagg cgcatgccca
    10981 gagacagtcc acgggaagcc agcacgtctt taatctcagt aagagatttt ttaccaaggt
    11041 taggcgtttt aaggagctca acctcggtac gctgtaccag atcaccgata tagtggatag
    11101 cttctgcttt aaggcagtta gcagagcgga cagtcaattc cagatcgtca acagggcgca
    11161 gcaggatcgg atcgaactct ggtttctctt ctttcacttc aggctgacgt acatcacgta
    11221 agtcaacgaa agcttccagt tgttcagcca gaatggttgc cgcacgacga atcgcctctt
    11281 caggatcgat tgtgccgttg gtttccattt cgatgaccag cttgtccagg tcggtacgct
    11341 gttctacacg cgctgcttca acattgtagg caatacgctc cacagggctg tagcatgcgt
    11401 cgaccagcag acggccgatt gggcgctcat cttcttccga atgaattcgg gtagaagccg
    11461 gcacataacc acgaccgcgc tgaactttga tacgcatgct aatagacgcg ttctcatcgg
    11521 tcaggtggca gatcacgtgc tgcggcttga cgatttcgac atcaccgtcg tgggtgatat
    11581 cggctgcagt cacagggcca atgccagatt tattcaaggt aagaataact tcatctttgc
    11641 cctgaactct caccgccagc cctttcaggt tgagcaggat ttccaggata tcttcctgaa
    11701 cgccttcttt ggtgctgtac tcatgtagta caccatcaat ctcaacctcg gtcaccgcgc
    11761 aacccggcat cgatgagagc agaatacggc gcagtgcgtt acccagagta tggccaaagc
    11821 cacgctctaa aggctcaagg gtcaccttgg cgtgcgtcga actcacttgc tcgatatcaa
    11881 ccaggcgcgg ttttagaaac tctgtcacag aaccctgcat tgtgtcctct ctttggtact
    11941 aagctttact tggagtaaag ctcgacgatc aggtgttcgt taatgtccgc agacagatca
    12001 gaacgctccg gcttacgctt aaacgtacct tccatcttgc cagcatcaac ttccagccag
    12061 gttggctttt cacgctgctc agccagctcc agagcggctt tcacgcgaga ctgcttcttc
    12121 gctttctcac gaatgcttac aacgtcattc ggactaacct gataagaagc gatgttaaca
    12181 acacgaccgt ttaccataat tgctttatgg ctaaccagct gacgtgcttc tgcacgagtg
    12241 gcaccgaagc ccatacggta tacaacgttg tccagacgac cttccagcag agccaacagg
    12301 ttttcaccgg tgttgccttt cagacgtgct gcttctttgt agtagttacg gaactgacgc
    12361 tccagcacac catagatacg gcgaactttt tgcttttcac gcaactgcac accatagtca
    12421 gacagacgcg gtttacgcgc accgtgctgg ccaggagctt gttcaatttt acacttggta
    12481 tcgatcgcgc gaacgccaga cttaaggaat aagtcggtgc cctcacgacg gctcagcttg
    12541 agcttaggac ccaaatatct tgccattttc tttctccaac aaacctggaa aacgaggcgt
    12601 tatacgcgac gttttttcgg cggacgacaa ccgttatgag ggatcggagt cacatcagta
    12661 atgttagtga tgcggaaacc tgcggcgttc agagcacgaa tagtagattc gcggcctgga
    12721 cccggacctt taaccataac ttccagattc ttgatgccgt attctttcac ggcgtcagcg
    12781 caacgctctg ctgcaacctg tgctgcaaac ggagtggatt tgcgagaacc acggaaaccg
    12841 gaaccaccgg ctgttgccca acccaacgcg ttaccctgac gatcagtgat agtcacgatg
    12901 gtgttgttga aagaagcatg gatatgagcc acgccgtcag agacttgttt tcttacacgt
    12961 ttacgtgcac gaattggtgc ctttgccatt attcaatcac cccgattatt tcttgatcgg
    13021 tttgcgcgga cccttacggg tacgtgcgtt ggtcttggta cgctgaccgc gaaccgggag
    13081 accacgacga tgacgcaaac cgcgatagca accaagatcc atcaggcgct tgatgctcat
    13141 gctgatttca cggcgcagat caccttcaac gacaaatttg gcaacttcgt cacgcagcgt
    13201 gtcgatttgt ccttcagaca gctcactgat cttaacatct tcagcgatac ccgctgcagc
    13261 caggatggct ttagaacggg tcttgccgac gccataaatc gaagttaatg cgattacggc
    13321 atgcttatga tcaggaatgt taatgcctgc tatacgggcc actatgcact cctactattt
    13381 aatatgtacg ttccatgctg aaaagcccgt tttcaggata ctcaaatgga aacgcacaga
    13441 catacaaaag attggctggc taatctagcc agctcaaccc aactttgcaa gaaaaatatg
    13501 cgaaaaaatc agccttggcg ctgtttatgc ttnnnnnnnn nncggctcgg cactgcaaat
    13561 cacacggatg acaccatcac gcttaacgat tttgcagtta cggcataatt tcttgacgga
    13621 agcacgaact ttcattttta ctctccgtaa cttctcgggc gaccaattat cggccgtagc
    13681 ctttcaggtt cgccttcttc aatgcagact catactgact ggacatcatc agagtttgca
    13741 cttgagccat aaagtccata atcacgacaa caacgataag cagtgaggtc ccaccgaagt
    13801 agaacggtac tttcattgca tcacgcatga actccgggat caggcagata aaggtaatgt
    13861 acagcgcacc aaccaaggtc aggcgggtca ttactttatc gatatacttc gccgtttgct
    13921 ctcccggacg aattcctggt acaaatgcac cggacttctt cagcttatct gctgtttcac
    13981 gcgggttgaa aaccaacgcc gtgtagaaga aacagaagaa gatgattgca gacgcataga
    14041 gtaacacata aagcggttgc ccaggctgca aatacagcga aattgttgtc agccagttcc
    14101 aaccagtacc gcccccgaac catgacgcga tggtcgccgg gaacagaata atactggaag
    14161 cgaagattgc cgggattacc cccgccatat tcactttcag cggtaaatgt gtgctctgtg
    14221 cagcatagac acgacgacct tgctgacgtt tcgcgtagtt taccacaatg cggcgttgac
    14281 cacgctcaac aaatacaaca aagaacgtca ctgcaaatac taatactgca accaacagca
    14341 acacgaggaa gtgcaggtcg ccttgacgcg cttgctcgat agtatgggca atggctggcg
    14401 ggagtcccgc gacaataccg gcgaagataa tgattgaaat accgttgccg atacctcgtt
    14461 cagtgatctg ttcgcccaac cacatcagga acatggttcc tgtgaccaga cttacaacag
    14521 cggtgaagta gaatgcaaag cccgggttaa tcaccaggcc ttgcatacca ggcatattcg
    14581 gcagaccagt agcaataccg atcgactgga atattgccag caccagagta ccgtagcggg
    14641 tgtactggct gatcttacga cgaccagact ccccttcttt cttaatttct gccaacgttg
    14701 gatgaaccac cgtcagtaac tggataatga tcgacgccga aatatacggc atgatcccca
    14761 gagcaaagat agaagcacgg ctgagagcac caccagagaa catgttaaac atctcaatga
    14821 tggtgcctcg ctgttgctca agcagtttgg caagtacagc ggcatcaata ccagggatcg
    14881 gaataaaaga gccaatacgg aacacaatca gcgcaccgat aacaaacagc agtctgcgtt
    14941 tcagctcgcc taagccacct ttggcacttt gaaaatctaa tcccggttgt ttagccatct
    15001 gctacttatt cctcgatttt accgccagca gcttcgatag cagcacgagc gcctttagta
    15061 acacgcaggc cacgaacagt taccggagtc gttacttcgc cagccaagat cactttcgcg
    15121 aactcgatct ggataccgat aatgttagcc gctttcagcg tgttcaggtc tacaacaccg
    15181 ccttctactt tagccaagtc agacagacga acttcggctg taatcgctgc tttacgagaa
    15241 gtgaagccga atttcggcag acgacggtac agaggcatct gaccaccctc gaaaccgcga
    15301 cgtacgccac cgccagaacg agatttctga cctttgtgac cacgaccacc ggttttaccg
    15361 aggccagaac cgataccacg acccaggcgt ttacccgcct ttttggagcc ttcggccgga
    15421 gacagagtat ttaaacgcat ctcttactcc tcaactttaa ccatgaagga aaccgcgttg
    15481 atcataccgc gaatagcagg agtatcctcg cgctctacgg tgtgaccaat acgacgcaga
    15541 cccaggccaa gcagcgttgc cttgtgtttc ggcagacgac cgattgcact gcgggtttga
    15601 gtaattttaa tagtctttgc catggtttat ttccccagaa tttcttcaac ggatttacca
    15661 cgcttggcag cgaccatttc tggagaattc atattttcca ggccatcgat agttgcacga
    15721 accacgttaa tcgggttggt ggaaccatac gctttagcca gaacgttatg aaccccagcg
    15781 acttccagaa cggcgcgcat tgcaccaccg gcgatgatac cggtaccttc ggaagccggc
    15841 tgcatgaata cgcgagaacc cgtgtgaaca cctttaacag ggtgttgcag agtgccgtta
    15901 ttcagcgcga cgttaatcat attgcgacgg gctttttcca tcgctttctt gatcgctgct
    15961 gga
//
LOCUS       DAWDRC010000019        15963 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1965_length_15963_cov_2.230890, whole genome shotgun sequence.
ACCESSION   DAWDRC010000019 DAWDRC010000000
VERSION     DAWDRC010000019.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15963)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1965_length_15963_cov_2.230890"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4719..4728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11130..11139
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12500..12509
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3911 a         4288 c         4058 g         3676 t
ORIGIN      
        1 gacttctttt ccggggatat caggtaacac aatcgcgctc gtgaaattaa ttcacaacaa
       61 ttcaagggct tcattttttt ggaagtcgcc tcgcagaagg cacagatcgc atagtgaaag
      121 cacttgcatt gcctcatagc tcttttacca ttagttatta atacgccgtt aagcaactca
      181 ggactctatt atggctttta caccttttcc tccccgtcag cctacggctt ctgcccgttt
      241 gccactgacg ctgatgacgc ttgatgactg ggcgcttgcc actattactg gcgcggacag
      301 cgaaaaatat atgcaaggcc aggtgacagc agatgtcagc cagatgactg aagatcagca
      361 cctgctcgcc gcccagtgcg acgccaaagg taaaatgtgg agcaatttac gtctgttccg
      421 cgacggcgat ggctttgcat ggattgaacg gcgcagcgtg cgtgaaccgc agctgactga
      481 actgaaaaaa tatgcggtgt tctctaaagt gaccatcgcg ccagacgacg agcgtgtgct
      541 gcttggtgtt gccggttttc aggcgcgcgc cgcgctggca aatctcttta gtgaactacc
      601 ttcgagagaa aaacaggtag tcaaagaagg cgcgaccacg ttgctatggt ttgaacaccc
      661 ggcagaacgt ttcctgatcg taaccgatga agctactgcc aatatgctga ccgataaact
      721 gcgcggtgaa gcggaactga acaatagcca acagtggctg gcattaaaca ttgaagcggg
      781 ttttccggtg attgacgccg ccaacagcgg gcagtttatc ccccaggcga ccaacctcca
      841 ggcgctgggc ggcatcagct ttaagaaagg ctgctatacc ggacaagaga tggtggcgcg
      901 agcaaaattc cgtggtgcca acaaacgcgc gctctggttg ctgaagggta gcgccagccg
      961 actgccggaa gcgggtgaag acttagagct gaaaatgggc gagaactggc gtcgtaccgg
     1021 tacggtgctg gctgcggtca aactggaaga tggtcaggtt gtggtgcagg ttgtcatgaa
     1081 taacgatatg gaaccggaca gcatcttccg cgtgcgtgac gatgcgaata cattgcgtat
     1141 cgagccactg ccatattcgc tggaagagta aatctctgta tcgcatcagg catttatcgc
     1201 ctgatgcgac actggtgtgt cttttcaggc ctacaacgac gcaccaatta cgcctgccca
     1261 atatacaaat agatcgccag aaagtggcac acactaccgc cgagcacaaa gccgtgccag
     1321 atggcatgat tgtatggaat gcgcttgcag acgtagaaaa tcaccccgag cgaataaacc
     1381 acaccgccta ccgccagtaa ggtaacgctg cccgccgcga gcttaactgc catttcataa
     1441 attaccacca gcgacagcca gcccatcgcc agataggtca ccagagataa aattttgaat
     1501 cggtgcgcga tggtcagttt aaacagaata cccagcaatg ccaggctcca gataacaatc
     1561 atcaacccgc tcgctaacgg agaatccagc cccaccagca aaaacggcgt ataggttccg
     1621 gcaatcaaca gataaatggc acagtggtca aatttcttca gccacatttt tgcccgttga
     1681 tggggaatgg cgtgatagag cgtcgaagcg aggaacagca ggatcatact gccgccatag
     1741 aggctgtaac tggttatcgc cgtggcgctg gcattgagat ccaccgcctg aaccagtagc
     1801 aacaccagcc caacgatacc aaacaccagc ccaatgccgt ggctgacgct gttggcaatt
     1861 tcctctgcca gcgaatatcc ctgcttaatg aggggcttct gaaccataac ttactccgga
     1921 aaaacgtaca cgcacatgta tacctctcca gcgtaactga gaatagttcc agtgaacacc
     1981 tgttagctaa aataaattct gatttgagat taatctccga aaaatcatga aattaaatgc
     2041 gaaatttcaa ctaacaggcg tgagttcaat ttaaaggcat ttaaattcaa tcacataaaa
     2101 ctgtgtctgg tcgggataga tgtcggcaat gacctttttc agttcagtca gggtcatatt
     2161 ttcctgcttt gcatgttttt ccgtcagcgt atccagcgtt acggttgagg ttgcggtgac
     2221 ttcaatcgtg caaaaataac cgtcatcttc aaaccgtccg acacgaagca catcacccgt
     2281 tttgaagtgc gattcagact cgtcgcggat ggtgatggtt ttacgcccag ccagaatgtc
     2341 atcctggaaa cgttgaaaaa aagtgatgtc gtttggctgc atggtactat ttcctgtaag
     2401 aattgactca tctggagcct atgatagtga aaaaactcac cttaccgaaa gatttcttat
     2461 ggggcggcgc agttgccgct catcaggtcg aaggcggctg gaacaaaggc ggcaaagggc
     2521 cgagcatttg tgacgttttg accggtggcg cacacggcgt gccgcgcgaa atcaccaaag
     2581 aagttgtgcc tggcaaatac tatccaaacc atgaagccgt tgatttttat ggtcactaca
     2641 aggaggacat caagctattt gccgaaatgg gcttcaaatg ttttcgtact tccatcgcct
     2701 ggacgcgcat ttttccaaaa ggcgatgaag ctcagccaaa cgaagaaggg ctgaagttct
     2761 acgatgatat gatcgatgaa ctgctgaaat acaacatcga accggtgatc accctctccc
     2821 actttgaaat gccgctgcat ctggtgcagc aatatggtag ctggaccaac cgtaaagtgg
     2881 ttgatttctt tgtccgtttc gcggaagtgg tatttgaacg ctataagcat aaagtcaaat
     2941 actggatgac cttcaacgaa attaacaacc agcgtaactg gcgtgcaccg ctgttcggtt
     3001 actgctgctc cggcgtggtg tataccgagc atgaaaaccc ggaagagacg atgtatcagg
     3061 tgctgcatca ccagtttgtc gccagcgccc tggcggtgaa agccgcgcat cgcatcaacc
     3121 cggagatgaa agtcggttgt atgctggcga tggtgccgct ctatccttac tcctgtaacc
     3181 cggacgatgt gatgtttgcc caggagtcga tgcgcgaacg ttacgtcttt accgatgtgc
     3241 agttgcgtgg ctattacccg tcctatgtgt tgaacgagtg ggaacgccgc ggatttaaca
     3301 tcaaaatgga agacggcgat ctggatgtac tgcgcgaagg cacctgcgat tatctcggct
     3361 tcagctatta catgaccaac gcggtgaagg ccgaaggcgg caccggcgat gcgatctctg
     3421 gttttgaagg cagcgtaccg aacccgtatg ttaaagcgtc tgactggggc tggcagattg
     3481 atccggttgg tctgcgttac gcgctttgcg agctgtatga gcgttaccag aagccgctgt
     3541 ttattgtcga aaacggtttt ggcgcttacg acaaagtgga agatgatggc agcatcaatg
     3601 acgattaccg cattgactac ctgcgcgccc atattgaaga gatgaaaaaa gcggtgactt
     3661 acgatggcgt ggacctgatg ggctacacgc cgtggggctg catcgactgt gtgtcgttca
     3721 ccaccgggca gtacagcaaa cgctacggct ttatctatgt gaataaacat gacgacggta
     3781 ctggcgatat gtcgcgttca cgtaagaaga gctttgactg gtacaaagag gtgattgcca
     3841 gcaacggcga gaatctgtaa ttagccgatg tgcctgatgt gctccgctca tcaggcctgg
     3901 cactctttta atatattgag ctcgcaatat tttatattac gcctcagccg aaacacacaa
     3961 agcgcacttt attaacaagc gaaaaaggcg ccgaagcgcc tttagaagat agtcgaatca
     4021 gtaaattact ggtattcgct aatcggtacg caggagcaga acaggttacg gtcgccgtaa
     4081 acatcatcca gacgtttcac ggtcggccag tatttgtctg ccacgcctgc cgggaatact
     4141 gcaacttcac ggctgtacgg atgcgcccac tcggcgacca gttcgccctg aatgtgcggc
     4201 gcgttcacca gcgggttatc ttccagcggc cagacaccgg ctttcacctg gtcaatttct
     4261 gcgcggatag ccagcatcgc atcgataaag cgatccagtt ccactttgct ttcagattca
     4321 gtcggttcaa ccatcagcgt acccgccacc gggaacgaca tggtcggcgc gtggaaaccg
     4381 tagtcgatca ggcgcttggc aatatccagc tcgctgatgc cggtttcttc tttcagcggg
     4441 cgaatatcga gaatacattc gtgcgccacg cgaccgtcgc aaccggtata cagcaccggg
     4501 aaggcatcct gcagacggct ggcaatatag ttggcgttaa gaattgccac ctggcttgct
     4561 tttttcagcc cttctgcgcc catcatgcgg atgtacatcc agctgattgg caggatagag
     4621 gcactaccga acggtgccgc agaaaccgcg ccctgacggg ttaacatgcc ttcgatttgc
     4681 accacgctat gacccggtac aaacggagcc agatgcgcnn nnnnnnnnga tcggtcccat
     4741 acccggacca ccaccgccat gcggaatgca gaaagtttta tgcaggttaa ggtgggaaac
     4801 gtccgcacca ataaagcccg gcgaggtaat gccaacctga gcgttcatgt tcgcgccatc
     4861 aaggtaaacc tgaccgccga actgatgcac gacttcacac acttcacgga tcgtttcttc
     4921 atacacgccg tgggtagaag gataggtcac catgatacag gagaggttat cgcccgcctg
     4981 ttccgctttc gcgcgcagat cagtcagatc gatgttgccg tttttatcac acgccacaac
     5041 caccacctgc attcctgcca tatgcgcgga agccgggtta gtgccgtgcg cagaagccgg
     5101 gatcaggcag atatcgcgat gcccttcgtt gcggctttca tgataatgac gaatcgccag
     5161 caggcccgcg tattcgccct gtgcgccaga gttcggctgc atacaaacgg cgtcgtaacc
     5221 ggtcagtttc accagccagt cagccagctg cgcgatcatc tgctgataac cttcagcctg
     5281 ttctggcggg cagaacgggt gcagttcggc aaattccggc caggtgatcg ggatcatctc
     5341 ggcggcggcg ttcagtttca tggtgcagga acccagcggg atcatcgcct gattcagcgc
     5401 cagatcttta cgctccagcg agtgcatata gcgcatcatt tcggtttcgc tgtggtagcg
     5461 attaaacacc ggatgggtga ggatttcgtc gtcgcgcagc atcgcagcct gaatagagcg
     5521 actgtcgtga gccacgtctt tgtccagtgt gtcgatgtcc agcccgtggt tatcgcccaa
     5581 cagcacgctg aaaagctgca tcacgttttc gcgcgtggtt gtttcatcaa gggtgatccc
     5641 aaccgcgttc agaatatcgc tacgcaggtt gatttcagcc gcttcggcac gcgccagcac
     5701 gcccgctttg tcggccactt ccacacacaa agtatcgaaa tagtgcgcat ggcgcagctt
     5761 cagacctttt tgttgcaggc ccgccgccag gatatcggtc agacggtgga tgcggttagc
     5821 gatacgtttc aggccaaccg ggccgtgata aacggcatac aggctggcga tgtttgccag
     5881 cagtacctgg gaagtacaaa tgttggagtt cgctttctca cgacgaatat gttgctcgcg
     5941 agtctgcatc gccatgcgca gagcggtgtt gccagctgca tctttcgata caccgataat
     6001 acggcccggc attgagcgtt tgtattcgtc tttcgccgca aagaatgccg cgtgtgggcc
     6061 accatagccc atcggtacgc caaagcgttg cgccgaacca aaaacaatat ccgcgccctg
     6121 tttacccggc gcagttaaca gcaccagcgc cataatatcg gcggcaacgc tgaccacaat
     6181 tttgcgtgat ttcagttcgc taataagcgc ggtgtagtcg tgaatttcac cagtagtgcc
     6241 gacctgctgt aacagcacgc cgaaaacgtc ctgatggtcg agcacttttt gcgcgtcatc
     6301 gacaatcact tcaaaaccaa aggtttcggc acgagtacgg accacatcca gcgtttgcgg
     6361 atgaacgtca gaagcaacaa agaagcggtt ggcatttttc agtttactga cgcgtttcgc
     6421 catcgccatt gcttcggcgg cagcggtggc ttcgtcaaga agagaagccg aagccatatc
     6481 cagcccggtc aaatccagcg ttacctgctg gaagttgagt agtgcttcaa ggcggccctg
     6541 ggagacttca ggttgatacg gagtatacgc ggtataccag cccggatttt ccagcatgtt
     6601 acgcaggata accggcggta gctgcacggc ggtgtaaccc atgccgatgt aagacgtgaa
     6661 gcgtttattg cgactggcaa tagccttgag ttctgccagt gcggcgtatt cggtcgccgg
     6721 tgcgccaacc tgcggtggtg tcgcaagctg aatatctttc ggcacaatct ggccggtcag
     6781 cgcgtttaac gattgtgcac caacggcatt cagcatttct tgctgttgcg cggcgtccgg
     6841 tccgatatgg cgttcaataa aagcgccgct gttttcaagc tggcttaacg tctgtgtcat
     6901 gagcgatggt tcctgaaacg tgcagtgaat tgtgaacctc tctccttgcg aagagagtga
     6961 gggtgaggcg taaatttcct caccctgatc ctctcccgca gaagaggaat aaagccgtta
     7021 ctcgtcttct aacaatgctt cgtatgcggt tgcatccagc agtgattcca gttcgctttc
     7081 atcgctggct ttgattttga aaatccagcc gcctgcatac ggttcgctgt tcaccagttc
     7141 cggagaatcg ctcagtgcat cgtttaccgc cacgatttca ccgcttactg gcgcataaat
     7201 gtctgacgcc gcttttaccg attcggcaac cgcgcagtca tcgcccgcgc taaccgttgc
     7261 gcccacttcc ggtaggtcaa caaacaccat atcgcctaac agctcctgag cgtgttcggt
     7321 gatgccaacg gtgtaagtgc cgtcggcttc tttacgcaac cattcgtgtt ctttgctgta
     7381 tttcagttct gctggtatgt tgctcatcaa tcaatctcca aaaaagtaaa tcacgcgacg
     7441 gctttgccgt tacgcacaaa aacaggtttc gtcactttaa ccggcatttc acggttgcga
     7501 atttgcacaa tcgccgtttc accaatacct tccggcacgc gcgccagcgc aatgctataa
     7561 cccagcgttg gggagaaagt accgctggtg ataatgcctt catgctggtt gccctgcgca
     7621 tcagtaaaac gtaccggcag ttcattacgc agcacgcctt tttcggtcat caccagacca
     7681 accagttttt ctgtgccatg ttcacgctgc gcttccagag cttcacgacc gataaagtca
     7741 cgatctgccg gttcccaggc gatagtccag cccatattgg cggctaaagg agaaatagtt
     7801 tcgtccatct cctggctata caaattcatg cccgcttcca gacgcagcgt gtcacgcgcg
     7861 cccaggccac atggcttaac gccagcttcc accagcgcgc gccagaaatc ggccgctttt
     7921 tcattgggca gcgcaatttc gtagcccgct tcaccggtgt aaccggtggt ggcaataaac
     7981 agatcgcccg cctgcacgcc aaagaacggt ttcatccctt ccaccgcctg acgctgggcg
     8041 tcattaaaca gtgtggcagc ttttgcctgc gcattcggcc cttgtacggc gatcatggaa
     8101 aggtcatcac gaacagtaat ttcgatgccg aaaggttcag cgtgttgggt aatccaggag
     8161 aggtcttttt cgcgggtggc ggagttaaca acgaggcgga agaaatcttc agtaaagtag
     8221 tagacgatga gatcatctat cacaccgcca gaggcattca acatccccga gtaaagggct
     8281 ttgccgcttt tggtgagctt cgccacatcg ttcgccagca gataacgtag aaactcccgg
     8341 gtgcggctgc cgtgaagatc gacgatggtc atatgtgaca catcaaacat tccggcatcg
     8401 gtacgtaccg catgatgttc gtcgatttgc gaaccgtaat gcagcggcat catccagccg
     8461 tggaaatcca ccatgcgagc gccgcaaagc gtgtgttgtt cgtacaaagg agtctgttgt
     8521 gccatcttgt cctcattgaa taagcggggc tgacaacttt ttcatggtga aattatcacc
     8581 acgaaaccca gcatcggagc cactcccggt ccccaacgca atcgttctct tttgcctgaa
     8641 cttaccaccg aaacagactg ttaaccataa ggttaaattg atcatcacat tagcttatgg
     8701 ttaaaaaacg ccaaaatcgt aacagaataa aaaaccaaaa aatgaacaaa tctctatata
     8761 aagataaata gatgagaaag ttaatttgaa taagacaaca ttaagaacta aaaaaatgtc
     8821 aaaaaacact aaatcaaaaa ttcgtggcat tagaaaatat aatgcgaaat cgcaggtgaa
     8881 attagtttat ttcaaatgag gaaaatctcc cggcgaaaaa accgggagat gaaagtgtga
     8941 tgggtatcaa ataaacagca gaagagaaat atttaacgca gccattcagg caaatcgttt
     9001 aatcccattg cctgacgaat aagttgtggc ttaacgccag gaagcgtatc agccagtttc
     9061 agaccaatat cacgcagcaa ttttttcgcc ggattagccc cggaaaacag atcgcggaat
     9121 ccctgcatac cagccagcat cagcgccgcg ctgtgcttgc ggctacgctc atagcgacgc
     9181 aggtaaatgt actgcccgat gtctttaccc tgacgatgca accgtttcag ttcagcaatc
     9241 agctctgcag catccataaa gccgagattt accccctgcc ccgccaacgg gtgaatggta
     9301 tgcgcggcgt cgcccaccag cgccagacgg tgcgcggcaa actggcgcgc ataacgcccc
     9361 gttagcggga acaccaggcg ctcactttca accttgcata agcccaggcg attatcaaaa
     9421 gcgatattta acgcgcgatt aaattcgtct tcactagcct gctgcatccg cagcgcttcc
     9481 tctggcgaca gtgaccagac aatcgagcaa agatacggat cgctaagcgg taaaaaggcc
     9541 agaatgcctt cgccatggaa aacctgccgc gccaccgcat catgcggttc ttccgtgcga
     9601 atggtcgcca ccagcgcgtg atgctgataa tcccagaaag tcagcggaat atcggctttg
     9661 ttgcgcaacc aggaattagc gccgtctgcg ccaatcacca gacgcgccgt taacatgctg
     9721 ccatctttca acgtcaggaa ggtttcattt tctccccagg cgacctgctg taattctgcg
     9781 ggggccaaca gagtgatatc tgacgactgc tgcgctttgt tccacagcgc gtagtgaatc
     9841 actgagtttt caacgatatg cccaagatgg ttatagccca tgctttgatc gtcaaacgag
     9901 atgtgaccaa aactgtcttt gtcccacact tccataccgt gatagcaact ggccctacga
     9961 gagagaatgt cctgccagac gccaagacga gtcagtaatt tttcgctggc ggcattgata
    10021 gccgaaacgc gcagttgtgg tggtgcatcc gccgccagag gttccggtac gcgctgctcc
    10081 agtacggcaa cgcgtaagcc gctaccctgt aagccgcagg caaccgccag cccgaccatg
    10141 ccgccgccaa caatggctac atcaacactt tgcattgttt attccttaaa accgccttca
    10201 acgcgccacc caaccaagag tgcgctgcgc cagcacatcg cgtgctgggg taaataattc
    10261 catcgtcatc agcccgatgt tgcgcccgac aaccagcggc gtccaacggt tggcaaaaag
    10321 atgtacaagg ctgtccgtga cgccgatggt tgcttcgcga tcgctctgtc gacgctgctg
    10381 ataacggcac aatacgccgt aatcacccat gtcttctccg cgttcctgcg cctgagtcag
    10441 ggtttccgca aggctcatga catcacgcat accgaggtta aatccttgcc cggcgatggg
    10501 atgcagagtt tgcgccgcat tgcccaccag cacggtacga tgggtaatag gtttggcggc
    10561 gcgggttagc gctagcggat aagcactgcg tttaccggca tgagtaattt tcccaagtcg
    10621 ccagccaaag gccgactgga gttcacggca aaacttctcg tcgctccacg acagcacctc
    10681 ttcgcgccgt tccagtggat gacaccagac cagcgaacag cgtccgtcag acatcggcaa
    10741 catcgccagc gggccatgtt tcgtgaagcg ttcaaaagcg cggccttcgt gcgcaacgga
    10801 agtcgcaacg ttagcaatca cagccagttg ttcgtaaggc tcctgctgcc agtcaacgcc
    10861 gcacacagtg gctaacgctg aatgggtgcc atcagcggct accagcacgc agcccgtcag
    10921 cgtctcgcca ctctccagcg tcacttcaac gtgactctga gcacgggcaa cgttagccac
    10981 gcgatcaggg caatgcagcg ttacgccagg tgctttgcgc agcaatgcaa acagccgttg
    11041 cccgacatta tgcaattcga caacctgccc cagcgccgcc agttggtaat cttctgcggc
    11101 gagggtgacg aatccggcat gaccacgatn nnnnnnnnna catgcacgct ggtgatggcg
    11161 gttgcgcaat cagccagaga ttgccagacg ccgatgcgcg ccagttgctg acaagtaccc
    11221 gccgccagcg caatagcccg tccatcaaag cccggatgag catgtgactc tggcgcagtc
    11281 gcttcaatca aatgtaccgg cagcgccccg tgacttaacc gggaaatagc cagcgccagc
    11341 gtcgcgcccg ccatgccgcc accgacgatg attacgctca ttgctttctc gcagcagcca
    11401 tcaacgcttc gatttcttcc ggctttttca ccacgctggc ggtgaggttt tcgttgccgg
    11461 tttcggtaat cacaatgtcg tcttcaatac gaatgccgat accgcgatat tgttctggca
    11521 catccgcatc cggcgcgata tacagaccag gctctacggt cagtaccatc cccggttcca
    11581 gaatgcgcga gcgatcctga ccataaacgc caacgtcatg gacatccagt cctaaccagt
    11641 ggctaaggcc atgcataaag aaaggacgat gggcgttctg agcgatcagt tcatcaacat
    11701 cacctttcag gatgccgagt tttaccaggc cgctaaccat gatgcgcacc acgtcaccag
    11761 tgacttcctg aatggaagtt cccggacgat acaggcgcag gctggtttca agagattcca
    11821 gcacaatgtc gtagatttca cgctgggctt gggtgaattt gccgttgacc gggaaggtgc
    11881 gggtaatatc gcccgcgtaa cctttgtatt cacaacccgc gtcaatcaac accaggtcac
    11941 cgtcacgcag ttcacactcg ttttcggtgt agtgcaaaat gcagccgttt tcaccgctgc
    12001 caacaatggt gttataggac gggtaacgcg caccgtggcg gttaaattcg tggtgaattt
    12061 cgccttccag atggtactcg aacatccccg gacggcattt ttccatcgcc cgggtatgcg
    12121 ccagggcggt gatttccccc gcgcggcgaa gtacggcaat ctcttctggt gatttgaaca
    12181 ggcgcatttc atgaacaaca ggacgccagt cgatcatcgt tgccggtgcg gtgagatttt
    12241 gccgcgaacc tttacgcagt ttctcaagcg cgctgttcac gatttcatca gcatatgcat
    12301 attcgccctg ggcgtggtaa accacatcca gaccattaag tagttgataa agttgctgat
    12361 tgatttcgct gaatgccagt gcgcggtcaa cgcccagttt ctctggcgcg gcatcctggc
    12421 ctaagcgacg accaaaccag atctctgccg tcaggtcgcg aacgcggtta aacaaaacgc
    12481 tgtggttatg agtgtcatcn nnnnnnnnng tgaagtacca gaagtcactg ttctgacgat
    12541 aggggtattc gctgtcggcg ctacgtgtta cttctggtgc agcaaaaatc agcgcggcgc
    12601 tgccgggttg catttgctct accagggcct gacggcgacg ctgaaattct tgccgggata
    12661 tatcactcat aacactctcc ttacgttttt tgtttttagt gtagagtcgg tttttgtact
    12721 tctggcgcgg tcggttgcgg atgagtaaag gtgtcgtggc ataacagcgc ggcaacacgg
    12781 acgtactcga tgatctcttc aagcgacatt tcaagctctt cctgatcttc gtcttcgtcg
    12841 taacccaact gcgcgatgtt acgcagatcg tcgatggctt caccggtttc gccggtcact
    12901 ttgtccagct tcggttgcgt aacgccaaga ccaagcagga agtgattgac ccaaccagcc
    12961 agcgcatcag cccgatcgaa aacgctgaca tcatcgccat caggcagata aagctgaaaa
    13021 aggaagccgt catcctgcag ggcatcgctg gtggcagagt gcattttgcg cagtgcctgt
    13081 gccagctcat gaccgaaagc catgccttcg ttcgtcaggt cgtgaagtag cggcagccat
    13141 gagctgtcat cgttaccgcc acatatcatc ccactgatta aaccatgcat ctcagcaggg
    13201 gtcagacccg tcccttgttg gttcagatac tggttcattt cgttgtaacc aggcatttcg
    13261 ttctgtatag acataagcat tcgtcgtcaa agggaggaat attcatgata tgctaccact
    13321 ttgggccctg atggaccaga aaagggcttg tctcttctca tcagggtagc tatagtgtcg
    13381 ccccttcgca gaccatgggt ctaaagacga aggcagcgca gtcaatcagc aggaaggtgg
    13441 catgtctgca caacccgtcg atatccaaat ttttggccgt tcactgcgtg tgaactgccc
    13501 gcctgaccaa agggatgcgt tgaatcaggc agcggacgat ctgaaccaac ggttgcaaga
    13561 tctgaaagaa cgcactagag tcacaaatac tgaacagttg gtcttcattg ccgcattgaa
    13621 tatcagctat gagttagcgc aagaaaaagc gaagactcgt gactacgcgg caagtatgga
    13681 acagcgtatt cggatgctgc agcagaccat agaacaagcg ttacttgaac aaggtcgcat
    13741 caccgaaaaa actaaccaaa actttgaatg acacttttcg gtttactgtg gtagagtaac
    13801 cgtgaagaca aaatttctct gagatgttcg caagcgggcc agtcccctga gccgatattt
    13861 cataccacaa gaatgtggcg ctccgcggtt ggtgagcatg ctcggtccgt ccgagaagcc
    13921 ttaaaactgc gacgacacat tcaccttgaa ccaagggttc aagggttaca gcctgcggcg
    13981 gcatctcgga gattcccttc ttacctggca ccagccatga cgcaactacc agaactccca
    14041 ctgacattat cccgacaaga aatccgcaaa atgattcggc aacgtcgtcg tacgttaacg
    14101 ccggaacaac agcaggaaat gggtcaacaa gccgctaccc ggatgatgac gtatccccca
    14161 gtggtgatgg cgcatacggt cgctgtattc ctctcttttg atggcgaact cgacacccag
    14221 ccactcatag aacaactctg gcgcgccggt aagcgcgtat atcttccagt tttgcatccc
    14281 tttagtgccg gtaacttgct gttcctgaat taccatccgc aaagcgaact ggtgatgaac
    14341 aggttgaaga tccacgagcc aaaactggac gtgcgcgatg tgcttcccct ttcccggtta
    14401 gatgtattga tcacgccgct ggtggctttt gatgaatacg gtcaacgctt gggaatgggc
    14461 ggtggttttt atgatcggac cttacaaaac tggcagcact ataaaatgca accggtgggt
    14521 tatgcgcatg attgccagtt agtagaaaaa ctcccagtgg agaaatggga tattccactg
    14581 cctgcggtgg ttacaccgtc gaaagtctgg gaatggtaag ggcgatacat cccgcatcgc
    14641 cctgattgac atcgttgatt ctttgaccta atttagtgag taagggtaag ggaggattgt
    14701 tcctcccctg tgactgactg ttaataagct gggaaactta tgagtaacag tacaatcagt
    14761 atgatgacaa gtcgcatcat aacccttctc cttcaagccc tcgctttggt gagggcttta
    14821 ccgttacagc cccatgctgc cctgccatcg taaatcccca ttaaataaac acaacgcatt
    14881 gatctgactt tgatttattt tctggagcag actcgcaaag tagaatgcgc aatgcggcaa
    14941 cggtgtggag aagggataaa aaaacgggca agtcagtgac ctgcccgtta attttcagag
    15001 aaggggaatt agtacagcag acgggcgcga atggtacccg gaatagcttt cattgcctgc
    15061 agcgcttttt cggcaacgtc ttcgtcggct tcaatatcaa taactacata acccatctgg
    15121 gcggaagttt gcagatattg cgcggcgatg ttgacgccct gctcggcaaa aattttgttg
    15181 agcgcagtca gcacacccgg acggttttcg tggatgtgca tcagacgacg accaccgtgc
    15241 agtggcagcg agacttccgg gaagttcacc gcagagagcg ttgaaccatt gtcagaatac
    15301 ttgatcaatt tacccgcaac ttccaggcca atattctcct gcgcttcctg agtcgaaccg
    15361 ccaatgtgtg gcgtcagaag gacattgtcg aattcacaca gcggagaggt aaatggatcg
    15421 ctggaatatc caccacagta ccgcgcgaag cattaatcag cagcgagccg ggtttcatta
    15481 gcgaaatctc tttcgcgccc atcatatttt tggttgacgg attctctggt acatgcagac
    15541 tcaccacatc gctcatattc agcaggtcag aaagatgctg tacctgagtg gcgttgccca
    15601 gcggcagttt gttttcaata tcataaaagt aaacatacat tcccagcgat tcagccagaa
    15661 tgcccaattg cgtaccaata tgaccgtagc cgatgatacc cagctttttg ccgcgcgctt
    15721 caaaagaacc cgccgccagt ttgttccaca cgccacgatg cgctttagca ttggcttccg
    15781 gcacgccgcg caatagcagc agcagttcgc caatcaccag ctccgcaaca gagcgcgtat
    15841 ttgagaacgg tgcgttaaat accgggatcc cgcgctttgc cgccgcatcc agatcaacct
    15901 ggtttgttct gatacagaaa cagccaatag cgaccagttt ttctgcggcc ttgatcacgt
    15961 ctt
//
LOCUS       DAWDRC010000020        15874 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_1989_length_15874_cov_2.460901, whole genome shotgun sequence.
ACCESSION   DAWDRC010000020 DAWDRC010000000
VERSION     DAWDRC010000020.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15874)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15874)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_15874_cov_2.460901"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2130..2139
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3072..3081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4521..4530
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7540..7549
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9444..9453
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11837..11846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14073..14082
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4117 a         4143 c         3778 g         3766 t
ORIGIN      
        1 gctcaaagaa gccacgaata cgatcaacaa tgctcgccgc ttcgccacag gtaatcgaca
       61 cttcatcacc gttgctcata tccaccagaa tctcacgacg ctcgcccggt gccagcgaaa
      121 gttgcttcac cgatacagga gcgggcagga atccctgatc gccagaaatc acatgtaacg
      181 ggcgaccatc gctcatctgt agttgatagc gacgagagtt cgacgcgttc aacagacgca
      241 agcgcaccca gccacgcgag acttcaacgt acgggctttg tacaccgtta accagcagcg
      301 tatcaccaac aaagccgccg cttcccggtt cgttgtattc tggcgtacca aagttatcca
      361 gccgtttatc ctgaataatg acaggaaaat catccacacc ataatggttg gggataggca
      421 gcgatttact gacttcatct tccaccagcc acattccggc aaggccgtta tagacctgct
      481 gagccgtgcg gttgggagta ttggcgtgat accacagagt agctgcgttc tggcgaatgg
      541 gcagtacggg tgcccagtca gcgtttggcg acatcatccg tgccggaccg cccatcagcg
      601 ggccaggtac ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg cggttgctgt
      661 aaataagctt aacatcgtcg cccttccaga cgcggatagt cggccccagg taacgaccat
      721 tgattcccca aaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag
      781 tcataaacag cggttgccca cggcgagatt caagcagcgg cggaacgggt agcggttgct
      841 gttgcccggc tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca atccccgatg
      901 cctgaatgaa ctgacgccga ctgagtgaca tatttgctcc atgtaaaact ggctaaagta
      961 ataccgtgca ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa cttttccggc
     1021 ggcttcgcgt tctgcgactt ctttatcgag ctcggcgatt ttttgttcca ttatcgaacg
     1081 acaatgggca gccaactcgc gaacctgatc tttgccatac tgactgacgt caattggcgg
     1141 cagcatttcg acaatcacca gaccgttgtg tagccgatta agattaatct tattcgaagt
     1201 tgtagagacg cacacgggta taatcgggac gcccgccgca attgccgcgt gaaatgcccc
     1261 ggtcttgaac ggcagcaggc cgcgaccacg gctgcgggtt ccttccggga acatccagat
     1321 ggaaatacgg cgttttttga agtgattcac tacttccgca atagtgccat gagctttagt
     1381 gcgattgttt ctgtcgatca ataagttgcc ggttaaccag tacaactgcc cgaagaaggg
     1441 gatccacagc aagctctttt tacctaccgt cactgtcggc ggctgcacaa tgttcgatgc
     1501 cgtcaccatg tcataattgt tctggtggtt agcaatatag atagcattgc cgtagctttc
     1561 agcgtctgca ggtttgcggc actccacttt caggccgaaa agcggcgcaa gacggccaaa
     1621 catatgccca aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga
     1681 gccgaataca cagactaaga tgctgtaaat cacggtaata ataagacgaa agatatatag
     1741 cataacaccc tctgaaggtc tgagagactg gcattgtacg tcacctgaaa atcaggttag
     1801 ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc atgaataaac
     1861 aacggtttca agggaatttt tatcgtttcc ggggattgaa tacgctgccg gatgacgact
     1921 taacgtttca tccggtgttc cttgcaagcg ggaggaaaca gcgccctccc cggcatatta
     1981 ctcttcgcta tcaccgctgc tggcacggcg aggagagtcg atctcaacac gatcgatacg
     2041 ctgcaaaccg cgcatcaacg taccgcggcg tccacgttcg ccagtgactt tctgtagctc
     2101 ttccggacgc agtttaattt tgcgtttccn nnnnnnnnnc caacatgaat ggtcagcgtg
     2161 ctttgcggcg gcagaacgta cagttgcgcc aggccatcct cgccacgcgc ggcttctgcc
     2221 gatggaatgt tgataatctt gttgcctttg cccttcgaca gctgcggcag atcgcttacc
     2281 gggaacatca acatacggcc tgcctgagtg attgccagca gcatatcgga agcatcttca
     2341 atcaccaccg gcggcataac atgggcattt tccggtaagg tgatcaaagc tttacctgca
     2401 cggttacgcg ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc atcggaagcc
     2461 atcagcagtt tctgatcgtc gctttccatc agcatatggt caacggtcgc tccaggcggc
     2521 aacgttaatt tgccggtgag cggctcgccc tgaccacgcg ccgacggcag cgtaatcggg
     2581 tcgatggcat agctacgacc ggtggaatca acaaacacta ccggttggtt gctcttacct
     2641 ttcaccgccg ctttgaagct atcacccgct ttataattca ggcccggcgc gtcgatatca
     2701 tggcctttag cgctgcgtac ccagcccatc tgcgacagca caatggtgac aggttcagac
     2761 ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa cggcgaacga
     2821 cgttcgtcac cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag gttattcatt
     2881 ttacgctcgg aagccaaaat gccctgcaac tggtcgcgct ctttttccag ctcactctgc
     2941 tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac gcagtttcag ttcgaggatc
     3001 gcttccgcct gggtttccgt aaggccaaac cgcgacatca gcgccggttt cggttcatct
     3061 tcattacgaa tnnnnnnnnn natagttcag tcggcggcgc acggtatcgc ggcggaacac
     3121 cagccattcg gagaggattt ccagcaggtt tttcaccgcc ggacgaccat ccagaccgat
     3181 catattgagg ttaatgcgat agctcttttc cagatcggtg gtagcaaaga ggtggttcat
     3241 cacctgatcc atatccacgc ggttggaacg cggcacaatc accagacggg tcgggttctc
     3301 gtggtcagat tcatcgcgca gatcgtcaac catcggcagc tttttgttgc gcatttgcgc
     3361 agcaatttgc tccagtacgc gcgcacctga aacctgatgc ggcaatgcgc tgataaccac
     3421 cgcgccatct tctttcttcc acaccgcgcg catacgcact gaaccacgtc cgttctcgta
     3481 gattttacga atctcggcgc gcgaagtgat aatttccgct tcagtcggat aatccggccc
     3541 ctgcacgata tccagcagct gatcgagagt ggttttcggc tggtcgatta atgcgattgc
     3601 cgcctgagcc acttcacgca ggttatgcgg tggaatatcg gtcgccatgc cgacggcaat
     3661 accggtggtg ccgttaagca aaatgtttgg cagacgggca ggcagcattt tcggctcctg
     3721 caaagtgccg tcgaagtttg gcacccagtc agccgtcccc tgccccagct cgctcaatag
     3781 cagctcggaa tatttcgaca accgggattc ggtgtaacgc attgccgcga acgatttcgg
     3841 atcgtccggc gcgccccagt tcccctgacc atcaaccagc ggataacggt aagagaacgg
     3901 ctgcgccatc aggaccatcg cttcataaca ggcactatcg ccgtgcggat ggtatttacc
     3961 cagtacgtca ccgacggtac gggccgattt tttaaatttg gcgctggcat tcaggcccag
     4021 ctcagacatc gcatacacaa tgcggcgctg aacaggtttc agaccatcac caataaacgg
     4081 caacgcacgg tccatgatca cgtacatgga gtagtttaag taggcgtttt ccgtaaattc
     4141 atgtagcgca aggcgctctg ccatatcgct cattaattct gattcctcaa cttattcgcc
     4201 agccttcaaa ggggcaatat tgccggagat actacattat ctgccgcgtc gagtcacagg
     4261 agatcagcgt gaatagcgcc gccggacgtg ctccggcggc gataaaggca tcaatgtgcc
     4321 ttgatataca actctttgct gtagacataa tcctggggat tatgcagcgg aaaaccgcca
     4381 acgtagggtt taagcagttt gattaacggc tgatagtaga tgggaatcag cggtgcctgc
     4441 tggttgatga tcacttctgc ctgctgatac aacgcattac gctttgtcgc atcagtgatc
     4501 tgcgtggcct ggtttagtaa nnnnnnnnnn catttttcca gtgaccgacg ttttcttcac
     4561 tatcgctttt gagcgtgttc aggaagctgg aggcatcatt gtacgtcgca tcccacgact
     4621 gccgagacag cataaaatca ccggctcgtc gggcatcaag ataggttttc cactccattg
     4681 tgcgcagcgt cacctgtgca cccagccatt ttttccattc ggaagacaac gctatcgcgg
     4741 tcttttcatg cagatcgtac ttgttgtaga acagctcaaa gcgtagcgga tgagaggcgt
     4801 cgtatcccgc ctgtttcagc aagacttttg ccatcgcgac gcgctcactc attggctttt
     4861 gcagttcatc gaacgtcgtc gcgctaaagc cttttacctc tggcggcgtc agcgtggttg
     4921 cgggcgttct caaccccagt accttttgcg caataagctg tcgatcaacc gtaagatata
     4981 gcgcccgacg cacccgcaca tcgttaaatg gcggtttctc aaggttaaag ttgtaatatt
     5041 cgctgttcag acgcgggata attcgtagct cgccaggcag tgatttttca atggcgggaa
     5101 tttgctgcgc cggaacccag gtgagatcga cctctcccgc acgatagcgg ttatagccgg
     5161 tgaccgaatt atccagcgcc agatactcaa cctgttgcaa tactgtatgt tgcgcatcgc
     5221 ggtactttgg atttttgcgt gcagtaatct tttcgttaac tacccattta tcaagcacaa
     5281 aggcaccgtt gtaaaccatg ttctctggct tactccagct atcgccatgt ttagcgatga
     5341 catgatgagg aaccgggaac agcgtcggcc aggcgagcat cgtcgtgaac caaggaactg
     5401 gctgctcaag cgtaacttca agagtacgat catccatcgc tttgacaccc agcgatgtaa
     5461 catctgcttt acccgcaaca atagccgcgg cattgttaat gtgcgcctgt gccagatagc
     5521 cagcaaaagg gcttgccgtt ttcgggtcaa ccgcgcgctg ccagccgagg acaaaatcct
     5581 ctgccgtcag aggctgaccg tctgaccact gcaaaccgct acgcagatgg aaaatatagc
     5641 gcttgccgtc gtcaagtatc tcccagcgtt cagcctgagc gggctgcacc tggccttcac
     5701 cgtccatcca taccagacct tcaaacagat ccagcacaat ctgcgcggcg gtattctcct
     5761 ctaccttttg cgggtcgagc gtgcctgggt cgctatgatt gttgtaacga aagacttgtt
     5821 gcggggcgag cggtgtgttg gcgggaacgt cagcagcgta gagaggagcc gcacttacca
     5881 ggctgaccag ccataataaa tttcgcgtat acatggtatt ccttaccgga gaaattataa
     5941 gcccgacaac aatacgcctg tggcattgtt tgctcaaact tatcgcgagt gatttggctc
     6001 acactccggt aaagattccc catcacgcca acggcacata aatatccgtt tccagtcgcc
     6061 gctctgtcat cccttcggca agattggtgt agtgaaacag gatcggggct ttgcgcattt
     6121 tttcgccgct tgcaggtagc cagtggcgaa tgatgcccca tatcgtatgt gaaatatcgt
     6181 ccagctcgcc aacatggcgg gccacagcat aacgtccacc ggtaagttca ccattactta
     6241 cgccataacg attatcgggg atcggttcgc taacgctgcc gcagatatca aagcgaaacg
     6301 cttccggtgc ggtggtggca gggtcgtccc aggcgacgcc aaaagtctgg ctttggttaa
     6361 caggagaaag ccccgtctct ttacgccaca tgataaattt cgccacgctg tagttgagca
     6421 actcagggct acagcggtgc ggcaacatcg ccacagggat tgatgggaaa tcgataatat
     6481 ttacatccag tgtcaggttt gtcattttgc ctccctgcga aattattccc gggaacgcgt
     6541 cccgggagcg gtaacaatta cggatttact ttgcggatct gtttgacgtc aatttcaaca
     6601 gagttccagt ctttatcgac ttccccctga atctcaaccg tatctttcgg cgtcaccgtc
     6661 acgccgttcc agcgtttgtg gtcgatatca acattgatag taccgctggc atctttgaac
     6721 acgtagagat cgtcagagat gcgttcaacg atattgccgc gtagggttac ccaggtgtcg
     6781 tcacgcaggg attttgcgct ttctacggtc gttacgctgc cgttcggccc ctggaatcct
     6841 ccggcctgac tttgcgttgc cgatgggcca gaaaaacctc cctgctctgc tgccataacc
     6901 ggtgcactgc acagggccat tactgcgatt actgctgcga attttttcat gtttattact
     6961 ccctttaatg tctgtttccg agcatttaac aagatagtcc ttaacaactt cttaagggaa
     7021 aaaaataaaa tttaatgctg tacagagcgc gttacaacac ggtttactgg cagcaaatac
     7081 ggttatcgca gggatgaaaa aatgcgaatt ttactgatag aagatgacat gctgattggc
     7141 gacggcatca aaacgggcct tagtaaaatg ggttttagcg tcgactggtt tacacaaggt
     7201 cgtcagggaa aagaagcgct atatagcgcg ccttatgatg cggtgatcct ggatttaacc
     7261 ttaccaggca tggatggtcg cgatattttg cgcgaatggc gagaaaaagg tcagcgtgag
     7321 cctgtattga tcctgactgc gcgcgatgcg ctggcggaac gtgtagaagg gctgcgtctg
     7381 ggagctgacg attatctgtg taaacctttt gcgttgatag aagtcgccgc caggctggaa
     7441 gctctgatgc gccgaaccaa cggccaggcc agcaacgagc tgcgccacgg caacgtcatg
     7501 ctcgaccccg gcaaacgtat cgccacgctg gctggcgaan nnnnnnnnnt aacgctgaaa
     7561 ccaaaagaat ttgccctgct ggaattactg atgcgtaacg ctggtcgggt actgccgcgc
     7621 aaactgattg aagagaaact gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa
     7681 gtgcatgtgc atcatctgcg acgcaaactc ggcagtgatt ttattcgtac cgtgcatggt
     7741 attggctaca cattaggtga gaaatgaaat ttacccaacg tcttagtctg cgagtcaggc
     7801 tgacgctaat ctttttaatt ctggcctcgg tgacctggct gctttccagc tttgtcgcct
     7861 ggaaacaaac aacggataac gtcgatgagt tgttcgacac ccaactgatg ctgtttgcca
     7921 aacggttaag tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc
     7981 caaataaatt aaaacacggt catgttgatg acgatgcgct gacttttgcc atctttaccc
     8041 acgacggcag aatggtcctt aacgatggcg ataacggaga agatattccc tatagctatc
     8101 agcgggaagg ttttgctgac gggcaactgg tcggtgaaga cgatccctgg cgttttgtct
     8161 ggatgacctc acctgatggc aaatatcgca tcgttgtggg tcaggaatgg gaataccgtg
     8221 aagacatggc gctggcgatt gttgccggac aattgatccc gtggctggtc gcactgccga
     8281 ttatgttaat catcatgatg gtactactgg gtcgtgaact cgcgccgctg aacaaactgg
     8341 cgctggcact acgtatgcgt gacccagact cggaaaaacc actaaacgcg acgggcgtac
     8401 ccagcgaagt gcgcccactg gttgagtcgc taaatcaact gttcgcccgc acacatgcga
     8461 tgatggttcg tgaacgacgc tttacctccg acgcagctca cgaacttcgt agcccgttaa
     8521 cggcgctgaa agtgcaaacc gaagttgctc agctctctga cgatgatccg caggcgcgga
     8581 aaaaagcgct gctccaatta cattccggga tcgatcgcgc tactcgtctg gttgatcaac
     8641 tgctcacgct atcgcggctg gactcactgg ataaccttca ggacgtcgcg gagatcccgc
     8701 ttgaagatct cctgcaatcg tcggtgatgg atatttacca cacggcgcag caggcgaaaa
     8761 ttgacgtgcg actgacactc aatgccaacg gcatcaaacg caccggacaa ccgctattgc
     8821 taagtttgtt ggtgcgaaat ttgctggata acgccgtgcg ttacagtcca caaggtagcg
     8881 tggtagacgt cacgctgaat gccgataatt tcatcgtgag ggataacggc cctggtgtga
     8941 caccagaggc actggcgcga attggcgaac gcttctatcg cccacccgga caaaccgcta
     9001 ccggcagtgg acttgggcta tcgattgtcc agcgaatcgc caaattgcat ggcatgaatg
     9061 ttgattttgg gaatgcggaa caaggtggat ttgaggcgaa ggtaagctgg taacttctct
     9121 gtctatgaga gccgttaaaa cgactctcat agactttact aatagcaaaa tataaaccgt
     9181 ccccaaaaaa gccaccaacc acaacccaga caaataacga gcgagaatac atatagccaa
     9241 aactcgaaca tttcccgcat aaagagtttt cttaagataa gaataataag tggtgtcaga
     9301 agaaaaaatg ctgcaatcat tgcttgcaat atacgatctt cgcctgaaac ggaaggtata
     9361 ctgcaaatat tgtctcccga ctcaagcatc cagtcatact tattgaaagc aaaaaaagca
     9421 tctattaacc aataagtaag catnnnnnnn nnngtgcttc aaatattgat agtagttgtt
     9481 tttttaacat ctctgattac tcattaatca attttttgat atgttctcta atctctaaca
     9541 acctcatacc ataagagatg aatttataat catctgtatc acgcgcaggc aaattattaa
     9601 ttaaataaat aaaaaaaccc aaatctcgtt caacaccacg attatatcta ataccagcca
     9661 taatatactg ctcattcgtc atatacagtg ttgccgaaga gggataatca aaaagggcaa
     9721 gatctcttaa atgagtagca gcaattttaa aggtaaaatc atcttccatc aggcatgtcg
     9781 ctaactctaa ctgatttctg gtagttaatt ctgaagggtc aaggcctaga gtaatcgctg
     9841 cggttcgtat ttgtataccc attgagccaa atgatgtttt attttcaggc atatctaacg
     9901 atcccatcat tctttttgtt tcatatatct gaaacttata attttcaggc atacctcctg
     9961 actcaatcca ggccacgccg cccagtaact ctggacgaat tccagcggcg tatgcatgtt
    10021 taattatttt gtctctatta tattgtacat atgcatcttt ataagcattt aaataatata
    10081 ctcctgaacg gcgacgcgaa tcattttttc ctaacttcca gagtaaaacg tccatttcat
    10141 cccatttacg gaacttcgtt tgtggaattg tacaatatct attttttata gtgagatcgc
    10201 ttttcatatt taaaaaatcc tttcttccca acagctatat gattccgtac tcgcgatatg
    10261 gtgcttccat gttatatttt tataattaag aacaatgacc tcttccggtt gattacccgc
    10321 atgtgtaatt gagtgtggat aacaagtcag aatattcgat aaatatgccc cttgcaattc
    10381 aattgtgtaa aacctttcaa tacaaccgcc agaattaact ctataaaaat taaatacagc
    10441 tgataactct tcattttcag aaagagcggc atttaataaa ggggaggatt tatctattgg
    10501 cttgtaaaat tttatggggt gatgcactac attatactct cgacttaaag aatggtcgaa
    10561 ctgtaaaaca aaaatttcat ccacatgatt tatttgatat ttattaccaa tagatgcata
    10621 cgaaccacaa ccctcagaga tcaaaccttg tattttccct ttaattgata agtatatcat
    10681 atttgacata tatattctcc ataatcattt tttccgaaaa actcctgtat catttgcata
    10741 atacatctct cctgtgggag cttatgggaa gttgatttaa gtattgtcat tttcagaaaa
    10801 tatttatccc gcatcggtga atcagagaaa gatcagactt ttgctaaatt cgcaaaagac
    10861 tttgcacatt ttgctaattt caccgtaccg ctctgtgacg tactatagtc ggcaaacgtc
    10921 tcaccttgag gttaaaaaat gagcaacatc ctgattatca acggcgcgaa aaaattcgcc
    10981 cactccaatg gtcaactgaa cgacaccctg accgaagtcg cggatggcac actgcgcgac
    11041 ctcgggcatg atgtccgcat tgttcgcgcc gacagcgact acgatgtcaa agcggaagta
    11101 caaaactttc tctgggctga tgtggtgatc tggcagatgc caggctggtg gatgggcgcg
    11161 ccgtggacag tgaaaaaata tattgatgat gtattcaccg aaggtcacgg gactctgtat
    11221 gccagcgatg gtcgtacccg caaagatcca tcaaaaaaat acggttccgg cggcctggta
    11281 cagggcaaaa aatatatgct ttcactgacc tggaacgcac caatggaagc cttcaccgaa
    11341 aaagatcagt tcttccacgg cgttggtgtt gacggtgtgt acctgccgtt ccataaagca
    11401 aaccaattcc ttggcatgga accgctgccg acatttatcg ctaatgacgt gataaaaatg
    11461 cctgatgttc cccgctatac tgaagaatat cgcaagcatc ttgtggaaat ttttggttaa
    11521 ctagagctct ggctttagaa ggagttaacc atgattacag taatcgcaga aatccgtact
    11581 cgtcctggtc aacatcaccg tcaggcagtg ttggatcagt ttgctaaaat cgttccaacc
    11641 gtactgaaag aagaaggttg ccacggctat gcgccaatgg tggattgcgc tgctggcgtg
    11701 agtttccagt ctatggcacc ggattctatc gtgatgattg agcagtggga aagcatcgcg
    11761 catcttgaag cgcatctgca aaccccgcac atgaaagcat atagcgaagc cgtaaaaggt
    11821 gacgtgctgg agatgannnn nnnnnnagat gaatattcgt atcctgcagc cggggctttg
    11881 acatttctaa aaagaggccg gtgctgctac cggtctcagc ccagattagc cattctttct
    11941 tcccgttttc agtcctcgat ggaaatcgtt aattcgctta atggttttaa ccgcacgctg
    12001 cggcactgcg gaagctaccg accagacaat catttccaga cacaacaata ccgtaccatg
    12061 tagcggcgtt ttcccttttt catccccgcg cggaacatga atcacgatac tggcgtcttt
    12121 gctaaaacgt gaatccagcg cgtttgtcag caaaataaca gggatcccca gccgcctcgc
    12181 ctcacgaagc gttgtcagcc cttcccggtg cgcagatttt tgcgccatca tgatcagaac
    12241 atcgccacgt tgaagcgcga tcagttgctc ggcaaggcca atacccgtac ggtttagtgc
    12301 cgtggcaggc aaacctatgc ggttgaatag tcgtgcggta tagtccgcca gaatacccga
    12361 agcgccaatg ccaaaaattg cgacttgcca tgcctgcacc aacaacgcta cagcttgcgc
    12421 aagcgcgtgg cggttttcag gctctgacag gacattacag gtatacaaat gcccttcaag
    12481 aacaaagtcg attgcggtgt taacatcgct cgtcaagtta ctcaccgtcg tagacatctt
    12541 ttcgctggaa ctaagggctg gaccaagcca ctgttccaga gtgcgtttca gatcacgcag
    12601 cccggcaaac cccagggcct gtatagccct gataactgtg gcatcggaag tgttcaacgt
    12661 ggccgcaatt tccatagccg tttgctcgag taccacttca cgatgctcat taatgtagct
    12721 ggcgacaact cttaacccag gcgttaactg actcgcgcga gtgcgaaaac gctcaccaaa
    12781 aatatcaacc cgcactttat ctttctggat catttagcct ccggtagagg tcgccctgca
    12841 atttgtgatt gtatctgcgc agccatcagc cccagagatg caaaggagtt agaaattgcc
    12901 tcttcacgag aaatcagcac ataacgcccg gaacgacagg cagccagaaa ctgacaccat
    12961 cccggcatga ctttttccat tgctgccagc tcatcctgag gtttaccacc ggtgtcaccg
    13021 cgccaggttg caaaaacaaa atcggcatcc agttcaggta aacgctccgc gctcacatcc
    13081 attcgcccac cttcaggaat gctctcaatc agcggcggaa aacgaaaacc cgcatcgcgt
    13141 aatacccgcc ccagcgaatg atagctatgc atcacattaa tttttccctg gtttgcctga
    13201 atcaccgata ccgttatttt ttggctgtcc agcgtcgcct tcagcgcatt gatttgtgcc
    13261 tgatagcggc gttcaagtat cgccagctgc gactgcgttc ctgtaagttc tgccagcttg
    13321 cgatagattt ctggcgcacc ccctttgaga tgatcaatgc tcaccgtggg cgcaattttt
    13381 tcaagttgtt caatgggcgt attacgggtc ggttctgtga tgatcaggtc aggctttgcc
    13441 gccacaatcg cttcaatatc aatatcagcg gtaccaataa aggcgatcga tgaattatca
    13501 aagtcgacac ctgtcaataa cgcgccagat cgaatgaaat ggctaccgtc aggccgagtt
    13561 cgcccgtggc tggcgacagg aggtacaccg agctcaatca gaggaatagt gatatcaaga
    13621 tcgtgtaaag agacaattct ctttggatga gcaggaacag tgactttgcg ccctaaatca
    13681 tcagtaaata cctgaacagg ttctgttgca cgggaaaaaa atgacaacag tatcagcaga
    13741 gaaaaaaata aacgcatata actcccgtta aaaactgttc ctgcgctgcc agagcagtag
    13801 caaaaaaaat ggtccaccaa tcagtgagat aatgattccg gcaggcagtt gcaaaggcaa
    13861 aaaagccaaa cgaccaatgc tatccgccag aataactaac aacgcccccg ataccgcgct
    13921 ccccagcagt aatgtcgtct gcccgccacg tagaataaaa cgtgatatat ggggtgctat
    13981 caatccgaca aaaccgatgt taccaacaca cgaaacgcag acggctgtca ggattatggg
    14041 tgcggcgaca cgtaacaacg ccagacgaga acnnnnnnnn nnacccggtt gcaacctgat
    14101 gaccaagcag agcgatatcc gcagtcctgg cggtgaacag caataaaaga gcggctggca
    14161 acatccaaca ggctgaaata ccgactaaca tccagttagc tgcatgcagg cttcccgata
    14221 accacatcaa tgcagtttgt acatctcgaa catctgcagt ggtcataaat acaccgatac
    14281 cggcggcgaa aaaccaggaa acaccaatac caatcagaac aaatcgcggg cgggaaatat
    14341 cgcgcgcaca aaaaatcacg attaaagcca ccaacaggcc accagcaaga ccagccactg
    14401 gacgccagaa cattcccagc attggaaact ggaaaatcag ccacagcaca gccacgctac
    14461 atccttcttt gacgccgatt aacccaggat cggcaaggcc gttacgtgca atggattgca
    14521 ttgcggcccc tgccatacct aacatggccc cgcatagcaa tgccatgatg actctcggca
    14581 agcgaatatc ctgcacaata taacgtgcgt ctgtgcttac gttttctgga gagaacaatg
    14641 ctcgtcctac ttccgatgcc gggacggaga aagaaccctg catcagaccg aatatcagta
    14701 ttccaaccgc aaacagaaac aacccaccaa gcaacagcag atttttaggg cgtaccagtg
    14761 tcgaaaactt accaacacgt agtgggcgta gcccgacgtt cattatccga ctcatttgaa
    14821 catcctcgtc gccataatga caaaaacagg cgcaccaact aacgccgtca tgacaccggt
    14881 cgctaactca tagggcgtga aaagcgtccg ggcaataata tctgccagta ataacaaaag
    14941 cgcgccgaca caagctgaaa gagggagcag aacgcgcaga tccgctgata ccagacgccg
    15001 gacaatctgc ggtactagca ggccgacaaa accaatgggg ccagcaatcg atacggctgc
    15061 gccacagagc aacgcgatcg caagcagggt gaatgttcgc gtgcggagta cagagacgcc
    15121 cagcccctgc gccatgcgat cgcccagtgc caacatgtta agggagggtg caaggtaaag
    15181 ggctaacaca aatcccccca gagaaaacca ggcggacgta cccagcgtcg cccaatcctg
    15241 ccccgctaaa tcacctgcta gccatgtccg catcgccagc agtgtttgtt cgtcaagaat
    15301 caggattgca gccgttattg aggacacgaa cgccgatagc gccacgccgc ataaagtaac
    15361 tttcattggc gttaaccctg aacgccctgc ggaggaaagc agcaatatca acaggaacaa
    15421 taacgcgccc cccgtagatg ccagcagtgg gcgtccaacc ggaaaggcca gtccaagcgc
    15481 actggcggca acgacagcaa gagcggctcc ggcatttaag cctaaaatat gtggctcccc
    15541 caatgggtta cgaatgacag cctgcaacaa agcaccagca acaccaagag aagcaccggt
    15601 aagcaatgct gctgctaaac gcggcaaacg gagtcttaca attacgttgt gatcgaagtt
    15661 ttgcggatca aaatgaagaa atgcctgcaa caccgtttgt ggtgcaataa atcgggcacc
    15721 aatccctgga tataccacgc atcccaccag cagtaaggcg aggaaaatta cagtaaccag
    15781 ccctgtgcgc atcatacttt ttctacacca cgaaacggca tgaagaacgg ctttcccgtc
    15841 agaggattta ttgatgcgtg aacatcaacg tcaa
//
LOCUS       DAWDRC010000021        15170 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2082_length_15170_cov_2.459213, whole genome shotgun sequence.
ACCESSION   DAWDRC010000021 DAWDRC010000000
VERSION     DAWDRC010000021.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 15170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_15170_cov_2.459213"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8447..8456
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10749..10758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3707 a         3983 c         3627 g         3833 t
ORIGIN      
        1 cgagcgtcat atagccgcct gtgttgtaat gacaacgttt cgcggctatt cttgagttgt
       61 tcaggttcaa gattagcccc cgtgatgttg tcaggtgcat acctgcaacg tgcgggggtt
      121 ttctctctcc agcaaccaat gccaccaggg attaagcccc cgcaacattg cgcctcaccg
      181 gaacctttcc ggcttgccgc tgattttacg attgcttttt gccgtcagca cccggcaatc
      241 ttcctcattg cgtgatgcct tccagcttaa tttctttatt tgcaatagtt gcagctcata
      301 tccgggctga ctccagacta gtagtgaaat gcgcaatttt gtagaattgc gcctcctcgc
      361 gacctcccca gtgaatctgt tgcctcattt tttacatact gcgtattcga cttctccacc
      421 tgttgcgcaa gagaaactgg gtttattcat ttttgcgagg ccgacttctt tctggacagg
      481 actttatgca gcacaacaca ctaccgaaac acgatcagaa attgccgttt acacgctacg
      541 acttcggctg ggttttatta tgcataggca tggcgattgg tgccggaacc gtgctgatgc
      601 cagtacaaat tggcttgaag ggaatttggg tatttattac cgcagcgatc attgcttatc
      661 ctgccacctg ggtagtacag gacatttatt taaaaaccct ttctgaaagc gattcctgta
      721 atgactacac cgatattatc agtcattacc tggggaagaa ctggggaatt ttcctcgggg
      781 ttatctactt tttgatgatt atccacggga tttttatcta ctctctctcc gtggttttcg
      841 acagcgcctc gtacctgaaa accttcggtt taaccgatgc cgatctttct caatctctat
      901 tttataaagt cgccattttc gccgtactgg tggcgattgc gtctggtggt gaacgattac
      961 tgtttaagat ttccggacca atggtggtgg tcaaagtggg gattattgtc gtgttcggtt
     1021 ttgcgatgat cccgcactgg aatttcgcca atataaccgc cttcccgcaa gcctccgact
     1081 ttttccgcga tgtcctgctt actattccat tttgcttctt ttccgcggta tttattcagg
     1141 tacttaaccc aatgaatatt gcctatcgta aacgggaagc ggataaagta ctggcaaccc
     1201 ggctcgcgct gcgtacccac cggattagct atatcacgct cattgcggtg atcttgtttt
     1261 ttgccttttc gtttaccttc tcaattagcc acgaagaagc cgtttctgcc tttgaacaaa
     1321 atatctcggc actggcactg gccgcgcagg tgatccctgg gcatatcatt catatcacct
     1381 ctacggtgct gaatatcttt gccgtactga ctgcattctt tggcatttat ctcggtttcc
     1441 acgaggccat taaaggcatt attctcaatc tgttaagccg aattattgat accaggaaaa
     1501 ttaactcacg catgctgact ctggcgatct gcgcttttat cgtcattacg ttgacgattt
     1561 gggtttcgtt tcgtgtatcg gtactggtgt tctttcagtt gggaagcccg ttatatggca
     1621 ttgtgtcgtg cctcattccg tttttcctga tctataaagt ctcacaactg gaaaaacttc
     1681 gcgggtttaa agcctggatg attctgctgt acggcatttt gttatgcttg tcgccactgt
     1741 tgaagttgat tgagtaaacc ggagcgcatg gccccggttt tgtgagttaa cgctgcggat
     1801 tttcatcctg atcaacggca aaacaggcta ccagttgacc gccgtagtct tttagctgcg
     1861 gctgcaactg gatgcagggg ccgaagcgcc gacgacagcg ggcgttgaag gcgcagcctg
     1921 gcggtggatt cagcgggctt ggcagttcac cggtaagttt gatgcgttcg cgacgatcgt
     1981 ccgggttcag gcgtggtgtc gcggagagca gcgcctgagt gtacgggtgg cgcgggttat
     2041 tgaagatttg gtccttcgtc cccttctcca cgcagcggcc caggtacatc accatcactt
     2101 catcagcaat gtgctccacc accgacagat cgtgcgagat aaagacataa gatagcccca
     2161 gttcctgctg caaatccatc atcagattca gcacctgcgc acgcactgag acgtccagcg
     2221 cggaaaccgg ctcatcggca atcaccacat ccgggtcgag catcagacct cgggcaatgg
     2281 cgatacgctg acgctgaccg ccggagaaca tatgcggata gcggtcgtaa tgctcggttt
     2341 tcaggccgac tttcgccatc atcgacaggg ctttttcctg acgctgttct ttgcttaagc
     2401 tggtgttgat cagcagcggc tcttcgagaa tctgcccaac ttttttacgc ggattcagcg
     2461 aaccgtaagg gttctggaag acaatctgga ttttctgccg acgtagcttc tgcgcctgcg
     2521 gatcgtgctt gagcagatcc tgcccctggt aatacagttc gccgccggtg ggcgtttcaa
     2581 tcatcgtcag caaccgaccg agggtcgatt taccgcagcc agattcacct actactgcca
     2641 gcgttttgcc acgttcaagg ttaaacgaaa cgccatccag cgccttcacc agacgttccg
     2701 gcgcgaacat gcctttcttc accggataat gttttttcag gtcgatagcc tgcaacagcg
     2761 gttgttgcga ggtggcctca tgcgtactca tagtgtcggc ctcccggcat catcaagtgg
     2821 gtaatggcat ttggactgac gcccgtcagc gagcatattc agcgccggtt cttcagcacg
     2881 gcatttgtca gtggcgtagg ggcagcgcgg gttaagcagg cagccgttcg ggcggtcgta
     2941 cttgccggga acgacacctg gcaacgacgc cagacgttct ttgtcctgag caaattccgg
     3001 cagcgcacgc agcaatgcct gagtatacgg gtgacgcggc gcatggaaga tagcgtgcgc
     3061 atcgcccgtt tctactacct ggcctgcata catcacgata attttatgtg ccgcttccgc
     3121 caccagcgcc aggtcatggg taattaacac cagcgccatg ttctctttct gctgtagctc
     3181 cagcagcagt tcgataattt gcgcctgaat ggtcacgtcc agcgcagtgg tcggttcatc
     3241 ggcaatcagc agttttggtc gacaggcaat cgccatggcg atcatcacgc gctggctcat
     3301 gccgccggaa agctgatgcg ggtaaacatc cagacgcgat gccggatcgg gaataccgac
     3361 cagattcagc aggtcaatcg ctcgctgacg gcgggtactt ttgttgccgc cctgatgcac
     3421 cttaatcgct tccataatct ggaaacccac ggtgtagcac gggttaaggc tggtcatcgg
     3481 gtcctggaaa agcattgcca atgcggcacc caccaggttg cggcgctctt tttctgagat
     3541 acgctgcaaa tcctgaccgt taaactccag cttttccgcc attacgcggc ccggataatc
     3601 aatcagcccc ataatcgcca gcgaactgac cgatttacca gagccggact cacccacaat
     3661 cccgactact tcgccctgtt ttacgctgta gctgatgcgg tctacggcgc ggaacggtgc
     3721 gctttcgtcg ccgaaatgca ccgataattt atctacattt aataacgcca tctcgaacct
     3781 cttactgctt cagtttggga tcgagcgcgt cacgcagacc gtcacccatc aggttaaatg
     3841 ccagcaccgt cagcaggatc gccagacccg ggaaggtcac gacccaccag gcgctttgcg
     3901 cgaactgcaa cacgtcagag agcatggtgc cccactccgg cgttggcggc tgcgccccca
     3961 tgccgaggaa accaagagca gccatatcga gaatagcgtt agagaaaccg agcgacgcct
     4021 gaacaatcag cggcgcaagg cagttcggga agatgttaat aaacatctgg cgcattgccc
     4081 cggcacccgc cacgcgagac gcggtgacgt aatcgcggtt aacttccacc agcacggcgg
     4141 cgcgggttaa gcgcacataa tgcggcagcg caacgaaggt tagcgccagc gcggcgttac
     4201 caatcgacgg gccgaaaatc gccaccagca ccagtgccag cagtagactt ggcagcgcca
     4261 gcatcatatc gaccacgcgc atgatgatgt tatcgaccag accgccaaag taaccggcga
     4321 tcaggccgag aataacgccc ataatcagcg ataacaccac taccagacag ccaaccagca
     4381 gcgacaagcg cgcgccgtac atcagccgcg acagcacatc acgccccacg tcatcagtgc
     4441 ccagcaagtg cgccatgctg ccgccttcct gccaggctgg cggggcgagc agtgcatcgc
     4501 ggaactgttc cgctgggtta tagggtgcaa tccagttggc aaagatcgcg ataaacagca
     4561 cgatgacgac gtaaaccagc ccgacaaccg cgcctttgtt gcgtttaaaa tagtgccaga
     4621 actcctgtaa cggggtcatc ggcaccggtg cgctaatcac tttattttca gtaacctgtg
     4681 acatgatgtc cccttacttc ttatgacgaa tacgcgggtt caccacgccg tacagcagat
     4741 cgaccagcag gttgacgagg ataatcatcg tcgccaccag caatacgccg ccctgcacta
     4801 ccggataatc gcggcgttgc agtgcgtcaa tcaaccagcg ccccagaccg ggccacgaga
     4861 agatggtttc agtcagaatc gcccccgcca gcaatgttcc cacctgcagg ccgataacgg
     4921 tcaccaccgg cagcatcgcg ttacgcagcg catggacgat aatcacccgc atccgggtaa
     4981 gacctttggc acgcgcggtg cggatataat cttcgcccag cacttccagc atcgaggagc
     5041 gcgtcatacg cacaatgacc gccagcggga tggtacccag cacaatggcg ggcaggatca
     5101 tatgggcgac ggcatcgata aagttaccgt cttcacccca gatggcggtg tcgatcagca
     5161 taaaaccggt taacggattg gaatcatcga ggaacaccat gtcgctcaca cgaccggaga
     5221 caggcgtcag gttccagtgc accgaaacca gcatgatcag catcatgccc caccagaaaa
     5281 ttggcatcga gtagccggtc agcgccaggc caaccgctgt gtgatcgaaa atggaaccac
     5341 gtttaaccgc agccagcaca ccaaccggaa tcccgactgc ggtcgcaaaa atcatcgcgc
     5401 agacgccaag ttccagcgtg gcctgaaagc gcggcacgaa ctcttcccat accggaattc
     5461 ggcttttcat ggaaatgcct aaatcaccgt gcattacgcc ccaaatgtaa tggagatact
     5521 gctgccacat cggtttatct aagccgagtt cagccagcaa ttgcgcgtga cgctctgggg
     5581 agatcccacg ttcgcccgcc atgatcatca ccggatcgcc cgggatcatg tggacaaagg
     5641 caaatgtgag aagggtaata ccgataaacg tggggatgac gagtcccaaa cgtcggagaa
     5701 taaactgcaa cataacccgg attctctata gtgacccgca gcctggaact gcgtgtctgt
     5761 attgctcaca aatcaattcc cgcccttgtg ggacagggaa tgaagtattg ctgccggatg
     5821 gcgctgcacg cagaggcaca gcgccaccgg gtatgcagat tgttgacaaa gagcgctttg
     5881 tttatgccgg atgcggcgca aacgccttat ccgacctaca aaatcgtgca aactcaatac
     5941 attgcagagt tttcgtaggc ctgataagcg gagcgcatca ggcagttttg cctttgccat
     6001 cagtcttgta tggcttttaa ttattcgata gagacatttt cgaagtgatg tttgcctaat
     6061 ggatcaacca cataaccttt aacttctttg cgtaccggtt caaacacggt ggagtgagcg
     6121 atgatcagtg ccggagcctg atcgtgcatc acgacctgtg cttgtttgta cagttcaaca
     6181 cgtttattgt ggtcgtcggt agcacgcgcc ggttgaatca gatcttcaaa cggtttgtag
     6241 caccattttg agtagttgga gccttgttca gaggcggcgc agctgaacag ggtggcgaag
     6301 aagttatccg gatccccgtt atcgccagtc cagcccatca ttaccgtctg atgctcgcca
     6361 tctttcgcac gcttgaggta ctcaccccat tcgtaggtga cgattttggc ctgcacgccc
     6421 actttcgccc agtctgcctg aatcatctcc gccatgcggc gagcattcgg gttatacgga
     6481 cgttgtaccg gcattgccca caggtcgatg gagaaacctt tttccagacc cgcttctttc
     6541 agcaaggctt tcgctttttc aggatcgtaa gtgtagtcct gaacgtcatc gttatagccc
     6601 cacatggttg gcgggatcag gtttttcgct gatacgcccg cgccctgata aaccgctttg
     6661 atgatggcgt ctttgttcac cgcgtaggtc agagcctggc gaactttcac gtcatccagt
     6721 ggttttttct gcacgttata cgagagataa ccgacgttca gccccggcat ttccatcagg
     6781 ttgatggatt tatcctgctt catgcgggcg atatctgccg ggttcgggta cggcatcacc
     6841 tggcattcgt tcttctgcaa tttcgcgtaa cgcacggaag cgtcaggagt aatagagaag
     6901 accagcgtat cgatcttcgg tttggtgccc cagtagccat caaacgcttt atagcgaata
     6961 cgggaatctt tctggtactg ctgtaactgg aacgggccgg taccgattgg gttgaggtcc
     7021 agtttttccg gtgtaccggc tttcatcatc gcgtcagcat attcttttga cagaatagag
     7081 gcgaagtcca ttgccaggtc agcgaggaac ggcgcttccg ggcgagtcag cacaaactga
     7141 acggtgttat cgtccacctt tttcacttcg ctgatcagct ctggtaagcc catgccttcg
     7201 aagtattcgt agctgccgcc agagacttta tggtacgggt tttgcgcgtt tttctgacga
     7261 tcgaacgaga acactacgtc atcggcattc agttcgcgcg tcggtttgaa ttctttattg
     7321 tcgtgccact tcacaccttt acgcagatgg aaggtatagg ttttaccgtc ttcgctgact
     7381 tcccactttt cagcgaggcc cgggatcact tcagtggtgc cgattttaaa ttcaaccaga
     7441 cggttataaa gcggtacgga agaggcgtca taggtggtgc cggaggtaaa cagctgcggg
     7501 ttaaaccctt ccggagatcc ttctgagcaa taaaccagag ttttagcctg aacacttgct
     7561 gcgacggtca tagccaccag gctgagacca agcttcagca tccctgactt tttcaaggaa
     7621 atacgcatta ttctgctcca attgtgatgt ttgttgtttt aaccctttgc agtgggttgt
     7681 cgctgcctga ccttttttgt tttttgcccg gtcgggtcaa cgttatgagg tggggatgcc
     7741 gtactaatta acatcagtgt aacagtgcgg atcctccata aaatgcccct cgtgacctac
     7801 aatctgtcaa cagaatgtga aaacgtcaat acagccaacc gggatttaca ccaacggtga
     7861 gaatccacaa acaaagatta aaaaaacttc aaacagctat ttgcagcaga gaaatttgtg
     7921 ctacgccaac acaaccagaa caatcagctt aatatttagc aacatttggt gattaagttt
     7981 tatgcattta atgaaaaaaa tctgaggtaa aggtggatat ctgagcgatt aataccatga
     8041 acgctaaaac gcacagcgga aaatgccagc gccagccata agtaacgcaa aaaaagattt
     8101 aagcaaatat aaaaaaagac aatggtttct gtcacaaatc cgttaatgga tagtgggata
     8161 tggggcgcaa atttggcagg taaatgtgtg gtgtgtaggt cgaataaggt gttcacgccg
     8221 catccgacaa tgggccggca catttgcctg atacgacgct ggcgcgtctt gtcaggccta
     8281 cgtcgaatcg aacctccccg cccccggagg ttctcttcct catgagttac agatgcaaaa
     8341 aagcctgctc gttgagcagg cttttcgaat ttggtcggtg atagaggatt cgaacctccg
     8401 accccttcgt cccgaacgaa gtgcgctacc aggctgcgcc aatcacnnnn nnnnnnccga
     8461 atgcggggcg catcttactg cgcagatacg ccctcgtcaa tcccttaata gcaaaatgcc
     8521 ttttgatcgg cgagaaagtc agcaggccta gttagccgac gcctcttttg cctgcgggat
     8581 gtgacaccag ttgttgtttt cgttaattcc accatccggt gaagtatagc caagacaacc
     8641 catgatggta tcgtacagct ctacatgacg gcgtggcact ttcatgtcgg cttctttttt
     8701 cagctgcgca aatgcctgcg cattgaccgg attttccaga tatttatccg acatccagac
     8761 catcatcggc acacggaact gttccggcgg tgccagttca cgcggcgtgc cgtgcaggtg
     8821 ctcgcgttca ttaattgact caccgtggtc agctgcatag aacacaatcg ctttcttatc
     8881 gcgaacctga tcaatcacgc tggaaataaa gtgatccaca taggtcaccg agttgtcgta
     8941 tgagttgatc atctgcgctt tggtacagcc gctatcaaca ccaatacatt ccggcttcca
     9001 ctgcgcaaag ctgcgcgggt aacgctgggt gtagttaaag tgtgagcctt tggtatgcag
     9061 aatgatcaga tgcttaccat ccgggttgcg ccccagcgat tgctgcattt cgtctaccag
     9121 caacatatca tctaccggtt tgccacggtt acgtggctcc gcaccaatct gctcacgata
     9181 agcaatgttg tccgccatcg tattgctgta gaaccacatt tcgctttgca tagcgtacag
     9241 atcagaactg aatcctaact gcttcaatac cgcgaaaatg ttctgttctt ttaatgtgcg
     9301 ctgcggatta tcttctgctc caccctgacg tacaaacatg caacgcagtg agagtttggt
     9361 tgcagtatca caggagtaac cacggaacgc cgccagattt ttctcctgag ccagcttcgg
     9421 tgtggtattg cgttcatagc cgaaaatgcc catatgatcc cagcgcgtgg tttcaccaat
     9481 aatgaaaacg acatatgtat catcaacgtt ttgtggagcc tggtacgtaa acttcttcgc
     9541 agggttaagg agagaattgt tatcggaaga ttcatcgacc cgcgcccagg cataaagacc
     9601 taatgccgaa agccagtttg atggcagata agagttcgcg acgacgccgc cataactcgg
     9661 caaatcaaca cctgtcgccc tctccacttt cttctgctgg atatccagca aacgaatcgg
     9721 tgcccaaacc ataatacctg ccagtacgac gaccgccagg ctgcgaatac gttgccctgg
     9781 ggttcgcagt tgtcggagca aggtgtaacg acagcggtta ttccagataa ggataagagg
     9841 caacgcgctt acggcgatta accagagaat aaagttcaga ccaacaactt cttttgacag
     9901 gtcgatatcg gtggtcatga cggatgcgat gatgccataa ccaatgacta cattaaggaa
     9961 ggtcatgtaa tagctggcac ctgcggaaaa gagcaccacc agcgatgcca gaatacgcca
    10021 actgcggcgg ccaaacagcg aaagcagacg cagtaaaaag aaggtcacca gtacggtggc
    10081 ggccagttca acaacagcag aaatgccttt ccagacggta aaatcgtgcg catagctgcc
    10141 gaagcggcgg taaaaaaccg cgccattcat aaaaaggcca atatagattg caagcaaaaa
    10201 gctcagcttc tgctgtgtaa tcgatttgat gtatctcatg caaacaaacc ctggacaaac
    10261 aggtgataac accctgctta gtggcttttc aggcctggca ggggaaaagt gcggaggtag
    10321 tcagataagt aaaatctaca cgcgcgaata agtagaccac agcaaaggga aaaagtgtgg
    10381 ggaaagagtg tgcacgaagc acaaatttac aaaaatgcaa gtttgaaaag agactttagc
    10441 gttacaaaaa gcagtaaaaa gggctagtga gctagccccg gtgaagcgaa attattcgta
    10501 ctgaaccgta aaatcgaccg tcgcatctgc gcggccaatg gttgcatcag cacgcagcgg
    10561 cacgtagctg gcgatatagt tcagttgatg cgcgccatca ccgttaatcg cgaactcaat
    10621 cggcgtcggt gtcgtatttt tcgggaactg ttgtactccc gcagaggttt taattttcaa
    10681 ccccacgccc gtagcaccac ccgcatccag cgcaaaaaga tttggatcgt ccgcattggg
    10741 tgttccatnn nnnnnnnngt tccatccatg gtgacgtaag ccctgacaac ttcgctacag
    10801 tttttcagac cgatggaaaa agtagtggga ttaagttctg ttccgattct tactggcttt
    10861 ttgagaaccg ctaatcccat gtcaacttca acactgtctt ttaccagttc acaggctccc
    10921 tggctgattt taagagagaa tttcaacgtt cctttcgccc ctaagtctgt ggttgccgac
    10981 agtgacgaaa aggaaaaagg tagcaggcat aatgcaagta ttgttcgttt catcagtgct
    11041 aatccaattc aacatgcatt gttgccacac cgctataatc accggctgcg ggtaatttcc
    11101 cggttgtact aaccggataa gcagtaatgg tggttgttac tttacgtttg acatattcgt
    11161 acagtgagcc gggattcatt tctaataagc tattactatc attcggctta agaatattac
    11221 ctttaccatc ctcaattacc gcaccaacat ccgcgttgcc caaactgatt gcgctggggt
    11281 aagccgcgtt tggcgtacct tcaagactca gatagatatg gacaccatca gaaatattag
    11341 tacagttaaa cgttaattct ttttgtattt tctggacatt ttgtggcggc tgcccggttc
    11401 gacctttgaa atcatgggcc tgatactcac caaagtcgat atccaatgat gtccctgcgg
    11461 caagttcaca gccttgagtt acggtgacct ttcctgaaag aagcacctgt gatattggaa
    11521 ctgaactata aacattctcc ttttttgtac caaacacaga aactataata gtttgcggaa
    11581 tgttttgttc cccaacaaaa ggtttagcga tataaagtga gagctgtcct ttactccctg
    11641 tagtccagcc aaaagttggt tgatcgcact ctcttcctgg ctggctattt gatttattga
    11701 taaatggaac ctgtacattc cccacgtttg caatatatac ccgggttgaa acctcaagat
    11761 tattggtaat tttaaaatag gttgagttat gccccgccgc caatgttgac tcagttttat
    11821 atagcgttgg tcgtgcttct gtcgggttcg gtgatggaca ttcacaaaca ccagaatatt
    11881 tatcacccag tgcccattgg taaaaatcag ggtaggtctg cccagtaaca ttatccgtcg
    11941 gattttgaat atttttggtg aatgatgtaa caaaaataaa ggggtcacca cttgccgtcc
    12001 tgcacggccc ccaatccgca gcccatatat tcccggtaaa ggtgctcgtc cctaaaagca
    12061 ccatacctgc caacaagttt ttaattttca tctgcatatc cttatttatc gcataccgca
    12121 ttaaccatca cgatacccga attatccttt ttctctggca agcggaatgg cacgcggcaa
    12181 cgttgcgcct gctcgtgccc ccagctcacc tgcaattcgc cctcttctgg catcccgctg
    12241 atataaagct gtccttcttc acccacaatg ctgctggcag gtttgctctc atcacttaac
    12301 gctgcggtag caccaaacgg tactgcgttg ccattcgccc gtttcaaact catcagcaca
    12361 cgataaccga cacgcgtatc aaagcgggaa cggactatcg ccccacgcgt tggaacgact
    12421 ttctgaaacg cttcctgtaa ttcaacgttt tgccctaacg tgtcgctgcg caattccaca
    12481 atggtttcac gatatggcgt gagatacggc accactgcgt tgcccatcca gtcaacttca
    12541 acgccgctgc tatttactac atgtccacca tgcgcacccg gcgcagaaac aattgccaga
    12601 ctttcaccca agggttgcga gagagtgata ccttcactgt gaacaatgac tccaccacgc
    12661 acaccatagt taacttgttg gttatctttg gaatagttat aacccacgtc aatgttgcca
    12721 gaacctccca tataacccat gttgacacta ccactgttgc caaccccatt actggtataa
    12781 ccctgctgaa cgttatagtt caagttacga tcttccagta atgtgccgct gacagaggcc
    12841 tgttgtgacg tcttgccgtt ctggtcggtg gtcatacgat aactgcccca tgcgccaccc
    12901 agcggtattg agaggctaaa tgaccacagt cgatcgtctt catcccattc agggcttctc
    12961 gtccaggtat aggcgatact gtaattcaca cgaccaatat gaccgtgata accagcgctt
    13021 aaggagttct ccttaccatc aacgttccag taatcctgct gcgtcatatt gaagtaaaca
    13081 gaaccgtagt ctcccagttg ctgagtgatg ttaccttgta tctggctgcg cttatgataa
    13141 cgccgatagt cactgtcggc gtcactacgg acatcggtcg cttcctggaa ggtgtaataa
    13201 ccgctggttg agtacttgta gcccaacaga cgcaggtcgg taccaccctc aaaacttttc
    13261 gaatagagga aacgataaga ctgcccttca tctttttcat tgttgcgtaa ctgacttttc
    13321 gcctgggtga catccaccga taccgcacca atggtgccga agtttttccc cagccccaga
    13381 gctaaagcgt tgtagtctgc cgatagcaac gcaccaccat aggcggtcat tccccacggc
    13441 aagccgtaca tggcagtaaa ttgcccgaat cgcggtttac cgctgtcgta attaccggca
    13501 cgatactcac cggcagcaag gctgtatttc agataccctt cacgctggaa aatcgccact
    13561 gccgaaaatg gttgaataaa acgttgttca cttccgtcag attctttaat gatcaccgtc
    13621 aagtcaccgc tgccggaggt tgcgtagaga tcgttaatct caaatgcgcc cggctgaaca
    13681 aaggtacgat aaataacata tccgttctgt tcgatactca cttcagcgtt acttttggcg
    13741 atgccacgca ccaccggagc aaagccacgc tggctatccg gcaacatttc atcgtcggat
    13801 gccaataatg ccccgcgcat ttgtacgcta tcaaaaatct cgccaggcgt ggcggtatca
    13861 cccaatgtta tctgtgactt gagtggaata atcgcccggt ttaacgatgt accaacgtta
    13921 tcccattgat ttttgccatc ggtacggttc cagatactgt tattacgtaa acgccacggc
    13981 cccacgttca aaccgctgcg caggttcagg taataactgt cactggtcga atctggagat
    14041 gaaccggaac cttcgttgcg accattgctg ccactaaagc tgtagtccag taacagcgca
    14101 ggtattcctt cgtcccagcg agaaggatca accgtgccac gagcggtcat catcattgct
    14161 gcctgtggaa aggtaagatg caacgtctga gtattaaaat caaaacgact ggtggcctgg
    14221 ggaatgattt catcaaacgc tacgcaggct tccggtggca atatcttcag tgccggaaag
    14281 ctgtcgacgc gcacgcccat ttcttccagt tgaacacggc taaggcaagg cataagcgac
    14341 tcgccgctgg ctttgcgttg tgcttcgctg gcagtactaa aatccagcgt ccgggaagcc
    14401 attttttctt cgtttacaaa cacaaatacc cgatatttac cgggtatttg ctggccgaca
    14461 ggagagtcgt aaactgacaa atcgacgtgt tgatcaatac cctgaacatt ttcaagaaat
    14521 ttagggttga attttccgcc gtacgcaggt aacgcgctgg caatagttaa tgccaacagt
    14581 gagaaagaaa aatgcgctct gtagtgatca agaggtaaat gcatccaatg catagtaaag
    14641 cgccttgtaa aaagatcagt taatcgactt cgtcagcatt tcaccggcga caccgaaatc
    14701 attaataatg ctgtatttca cttcgttcgc attgcctgta ccggctggaa ggttaatgtt
    14761 taacgttcct ttagcgagga ccattcccgc ccgttcaatc ttttgtccat taatggacat
    14821 ctgattaaag gtcaggttat aagctgagtc gttattaacc acaatttggt tattttgacg
    14881 ctgaaatcgt aactgtttca ttgcttcaag gttgcttccc ggcaagcctt tcggacggta
    14941 gaacaacttc aaacgggtgc gaacggctat ctgtaagacg tttttatttt cattttcagc
    15001 acttttcgca ggaatcgctt ttacattaat ccagtaaacg gattcacgat ctttgggtaa
    15061 ggtatcttca atattaacaa tacgtaagac gttattttta tcaggatcaa gtttgaataa
    15121 tggcggtgta ataataaaag gcgttttttt atttccattc acatcataaa
//
LOCUS       DAWDRC010000022        14924 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2110_length_14924_cov_2.504809, whole genome shotgun sequence.
ACCESSION   DAWDRC010000022 DAWDRC010000000
VERSION     DAWDRC010000022.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14924)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2110_length_14924_cov_2.504809"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    537..546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1816..1825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5567..5576
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6174..6183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7876..7885
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8551..8560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3557 a         3900 c         3966 g         3441 t
ORIGIN      
        1 gttccagcca gcgggaaccg atattgccct tgccgaacaa taccaggccg atgcgttttt
       61 ccgcacggaa gacggactga tgcagcccct gaatcaggct ttcggtcggg ccggtgcgca
      121 gtactgccac caggctgatg ccatcatcag actgccaggt aaactcgacc ggctggcctt
      181 tcagttgctg ccagaagcgg tggcaatgca gcgggttacg ggtgacgccc gccccaacca
      241 tcgccaccag cgctagcccc tgacgcaggc gcagttcgcc aggtaatccc gcttcgtcga
      301 ggattttcag cgcactgtcg gccacttctg aggtgtagca aaattgcagc aactggcgat
      361 cgttatgcac gccaaccgcc agtgggcgca cctgagcacg tttcaggatc tggtcgatct
      421 ctttatgcgc cagtttgaaa tcctggctgg cgggcacctg aaactcaatc aagcagacat
      481 catcatggct ggtgacaata cgcgcaccag taccggaggc cagcccgcgt tcaatgnnnn
      541 nnnnnncgca gcgcgttcag cgcgtaaaaa ctcgcgggca tcaagccagg ccgctggcag
      601 cccttgttga ttaagcaccg cagacatcag gcgcgccgac cacacttcac cgtggcccac
      661 cacttccgca tacaccgcat cgttaatacc gctgtcgagc aacgcagcca ggtgctcaag
      721 atcgctgaca aaagcgctaa tgaggttatc ggcttcttcg gcgggtagca ggccgctaat
      781 cagatcgcac tgataacgac gtaatgtttg ttgaacctga tgcgcagaga gacgatcggt
      841 ctggcttagt ttcaaccagt taatcaactg gttagtggtg ctaccggcgg cggaaaccac
      901 catcatatcg tcaggctgag agtactccgc cataatgccc gcgacacgca aataacactt
      961 cacatcagcc agactactgc caccaaattt atgcagttga cgacctttcg cccctgcctg
     1021 cgcaatcaca ctcattttta ccccttgttt gcagcccgga agccattttc caggtcggca
     1081 attaaatctt cgccatcttc aataccggtg gagatacgca gcagcgtctc ggagatcccg
     1141 gcggcagcac gcgcttctgg cgccatgcct gcatgtgtca tggttgcggc gtgagagatt
     1201 aaactttcca ctccccctaa tgattccgcc agcgtaaaca acgacagccc gcccaggaaa
     1261 cgacgcagcg tctgctcatc gccatccagt tcaaaactca acattgcgcc aaagcctttt
     1321 tgctggcgcg cggcaatttc atgcccctga ttttccggca ccaacggctg ggtttgcaga
     1381 tatttcacaa tcgcctgcgc gttgcgctgc gccagctcca tacgcggcac cagcgttcgc
     1441 aacccgcgta gcagcagata gctgtcaaac gcgccgcccg tcacgccaat attgtttgcc
     1501 caccaggcaa gttcagtgac aacgtccggg tctttagcaa tcactacgcc tgccaccacg
     1561 tcagagtgac cgttcagata tttcgtgcat gaatgcaaca ccagatcggc acctaatgcc
     1621 agtggatttt gtaatgccgg gcttaagaag gtgttatcca ccacgctcac cgccccgact
     1681 tcccttgcca gatggcagat tttcgcaata tccacgacgc gtaacaatgg attacttggg
     1741 ctttctacca gtaccagttt ggggttttct gccagcgctg cccgtaatgc ctgttcatcg
     1801 ctttgatcaa caaacnnnnn nnnnncgata gctaccgccg tagcagtcgt gcggcgcaac
     1861 cagcagatcg ccaggtttca aaaagacggt cgttaccagg tgaatcgcgg acatgccggt
     1921 attagtaagt actgcaccag caccgccttc cagttctgcc agcgctcgct gaaccacatc
     1981 gcgcgttggg ttgccgcgcc gcgagtaatc atgcgcgcgc ggttcattaa atccggtaaa
     2041 gttataggta ctggaaagat ggatgggagg gacaacgcaa ccatactgtt cgtcgtcatt
     2101 taacccgcta cgcactgcga tggtggcctg tttacgcgtc atgtgatgaa gttccctggg
     2161 cttggtcggt gaaatgtcag gcaccagagt aaacattgtg ttaatggacg tcaatacatc
     2221 tggacatcta aacttctttg cgtatagatt gagcaaatct caaatagccg ttaaaattat
     2281 atgcattatc acgccgacag gtgcattaca cgatgtcacg gtaacgcctg tacggtaaac
     2341 tatgcgggtt tacggtcagt acccatatta attgtgtggt ctggcctcaa tttattgacg
     2401 aagaggatta agtatctcat ggctgaatgg agcggcgaat atatcagccc atacgctgag
     2461 cacggcaaga agagtgaaca agtcaaaaaa attacggttt ccattcctct taaggtgtta
     2521 aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca acctgcgtca cgcgaccaac
     2581 agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg ggcaaccttt gccggatgat
     2641 gccgacctgc gtaaagagcg cagcgacgaa attccggagg cggcaaaaga gatcatgcgt
     2701 gagatgggga ttaacccgga gacgtgggaa tactaaagcg gaaaagagcg gcgcggagtt
     2761 aaccagccgc ttttttcata catggacgtt taacgatgaa aatcaggcta ctgattctca
     2821 gcctgctggt ttctgtgcct gcatttgcct ggcaaccaca aaccggcgac atcatctttc
     2881 agatctctcg ctcatcgcaa agtaaagcga tccaactggc gacccattcc gattacagcc
     2941 acaccggtat gctggtgata cgcaacaaaa agccctacgt ttttgaagcg gccggcccgg
     3001 taaaatacac cccactcaag cagtggatcg cccatggtga aaagggcaaa tacgttgttc
     3061 gccgcgttga aggcggactg agtgttgaac aacagcaaaa actggcgcaa acggcaaaac
     3121 gttatctcgg taaaccgtat gactccagct tctcgtggag cgacgatcgc cagtactgtt
     3181 cggaagtggt gtggaaggtc taccagaatg cgctgggaat gcgtgtgggc gagcagcaga
     3241 agctgaaaga gttcgatctt agtaacccgc tagttcaggc aaagctcaaa gaacgctacg
     3301 gcaaaaatat tccgcttgag gaaacggtag tttcaccaca agccgttttc gacgcgccac
     3361 aactcaccac ggtcgccaaa gaatggccgc tgttttcctg gtaatacgac caaaattcag
     3421 gcacaaaaaa agcgccgtgc ggcgcttttt tcggaaatcc ggtcttattt gctgcccggg
     3481 atgttgaaac gcttgttgaa gcggtcaaca cggccaccgg tagcaacatc acgctgtttg
     3541 ccagtgaaga acgggtggca cttgctgcac acgtcgaggt tcaggtcatg accaacggtg
     3601 gagcggattt tcattacgtt accgcaagag cagctagcag taatttcttc gtatttcggg
     3661 tgaatatctt ttttcatggg aaaacctcgg tttaaggccg cgtcgctctt ccagccctaa
     3721 cgccagacac cacgcgatgt taaaagtata gcttcaatac gatcatttcg tacgaaggcg
     3781 cgaaatcata cagaaattaa ccagcgtatg caaactgatc cgcactcttc tacggcaatg
     3841 tgtatactaa cccaccgaat ttcaagtcag gatgatgcta tgcccgttgc ccacgttgcc
     3901 ttgcccgttc cgcttcctcg tacctttgac tatctgctgc cagaaggcat gacggttgaa
     3961 gccgggagtc gcgtgcgcgt gccgtttggc aaacaacagg agcgtatcgg tgttgtggtg
     4021 tcagtgagcg atgtcagtga gctgccgctt aacgagctga aagcggtagt tgaagtgctg
     4081 gacgttgagc cggtatttac tcactccgtc tggcgattac tgctctgggc ggcagattac
     4141 tatcatcatc cgattggcga tgtgctgttt catgccttgc cgattttact gcgccagggg
     4201 cggcctgcgg cgaacgcgcc gatgtggtac tggtttgcca ctgaacaagg tcaggcggtg
     4261 gatctgaaca gcctgaaacg ctcacctaag caacaacagg cgctggcagc gttacggcaa
     4321 ggcaaaatct ggcgtgatca ggttgccacg ctcgaattta atgatgccgc gttgcaggcg
     4381 ctgcgcaaaa aaggtctgtg tgatttagca agtgaaacac cagagtttag cgactggcga
     4441 acgaactatg ccgtttctgg tgagcggttg cgattgaata ccgaacaggc caccgccgtt
     4501 ggcgcaattc atagcgcggc agatactttt tctgcctggc tgctggcggg cgttaccggt
     4561 tccggtaaaa cggaggttta tctcagtgta ctggaaaacg tgctcgctca gggcaaacag
     4621 gcgctggtga tggtgccgga aatcggcctg acaccgcaaa ctatcgcccg ttttcgtgaa
     4681 cgttttaatg ccccggtgga agttctgcat tccggcctga acgacagcga gcgtctttcg
     4741 gcgtggttga aagcgaaaaa tggcgaggcg gcgattgtca tcggcacccg ctccgcgctg
     4801 tttacgccgt ttaaaaatct cggcgtgatt gttattgatg aagagcacga cagttcctac
     4861 aaacagcagg aaggctggcg ctatcatgcc cgcgacctgg cggtgtatcg cgcgcatagc
     4921 gagcaaatcc caattattct tggctccgca acgcccgcgc tggaaacgtt atgcaacgtc
     4981 cagcagaaaa aataccgcct gctgcgcctg acccgtcggg cgggcaatgc gcgcccggca
     5041 attcaacatg tgctggattt aaaaggtcag aaggtgcagg caggtctggc tccggcgtta
     5101 atcacccgta tgcgccagca tttacaggcc aacaatcagg ttatcctctt tcttaaccgc
     5161 cggggttttg cgcctgcgct actgtgccac gactgtggct ggatagccga atgcccacgt
     5221 tgcgatcact actacacgct gcatcaggcg caacagcatt tgcgctgcca ccattgcgac
     5281 agtcagcgtc cggtgccgcg ccagtgccct tcttgcggtt ccacgcacct ggtacccgtg
     5341 gggctgggca ccgaacagct tgaacagacg ctcgcgccgt tgttccctgg tgtgcccatt
     5401 tctcgtatcg accgcgatac caccagccgc aaaggcgcgc tggaacaaca actggcggaa
     5461 gtacatcgcg gtggcgcgag gattttgatt ggcacgcaaa tgctggcgaa aggtcaccac
     5521 tttccggatg tgacgctggt agcgttactg gatgtggacg gcgcgcnnnn nnnnnnaagt
     5581 ggcaggccgt gccggacgtg ccggtaaaca gggcgaagtt gttctgcaaa cgcaccatcc
     5641 tgaacatccg ctgttgcaaa cgttgctcta taaaggctac gacgcctttg ccgaacaggc
     5701 actggctgaa cgacggatga tgcagctgcc gccgtggacc agccatgtga ttgtgcgtgc
     5761 ggaagatcat aacaatcagc acgcgccgct gttcctgcaa cagttgcgta atctgatcct
     5821 cgccagcccg cttgcagacg acaaactgtg ggttctcggt cccgttccgg cactggcacc
     5881 taaacgtggc ggccgctggc gctggcaaat tttgttgcag cacccttccc gcgtgcgatt
     5941 acagcacatc atcaatggta cgctggcgct tattaataca ataccggatt cccgtaaggt
     6001 gaaatgggtg ctggatgtcg atcccatcga gggctaatgc gcaaacgatg cgaggcggat
     6061 cgaaaaattc aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta
     6121 tctgctaaaa atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgnnnnnnn
     6181 nnntacagcg actgtctccc gagcattaat gaatcccgat aaagtctccc aggccacccg
     6241 taatcgggtt gaaaaagcgg cccgggaagt gggttattta ccgcagccta tggggcgcaa
     6301 cgtcaagcgt aatgaatccc gtaccattct ggtgattgtc ccggatatct gcgatccctt
     6361 ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct
     6421 gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac
     6481 caagcaaatt gatggcatgt tgttgctggg ttcaaggctg ccgtttgatg ccagcattga
     6541 ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg aactggagct
     6601 gcctacagtt catatagaca atctgaccgc cgcatttgat gcagtaaatt atttatatga
     6661 gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccgc tgtgtcacta
     6721 ccgcctgcaa ggctacgttc aggcgctgcg tcgctgcggc attatggttg atccgcaata
     6781 catcgcccgt ggcgacttca ccttcgaagc cggaagcaga gcaatgcaac agctgcttga
     6841 tcttccacaa ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact
     6901 ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga
     6961 taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta
     7021 cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg
     7081 cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc
     7141 ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg
     7201 cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc
     7261 gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc
     7321 tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg acctttatcg
     7381 gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg caaagccaga
     7441 aagtgaccgg aaacggacta ccaccaaaac cagaagaacg ctggcgctac attaaagagc
     7501 tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga
     7561 aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg caggctgata
     7621 tgcgccagca gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc
     7681 gccagcaaac gctacagcgc cagcgtcagg cgcagcaatt agcggaacag caacgcctgg
     7741 ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg
     7801 cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc tactcagcag ccttaccagg
     7861 atctgctgca aacccnnnnn nnnnngcaaa cccctgcgca cacgactgcg cagtcgaagc
     7921 cgcagcaagc tgcgccggtt gctcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa
     7981 aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga
     8041 cggtacgtgc tcagctggcg ttcgaaggct ttgactcgaa aatcaccacc aacaatggct
     8101 ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc accctcaatc
     8161 gcttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt tgaaaccctc
     8221 aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg tttgtactct
     8281 gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc gtaacggcca
     8341 tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga aaggcaacgt
     8401 gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc
     8461 ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc agggccatct
     8521 ggtcaaagcc gccgttgagc tggcgaaaga nnnnnnnnnn tcgcatgctg cgcaaacttg
     8581 aagcattgct ggcagtcgcg gatgaaactg catcgcttat catcaccggt aacggtgacg
     8641 tggtgcagcc agaaaacgat cttattgcta tcggctccgg cggcccttac gcccaggctg
     8701 cggcgcgcgc gctgttagaa aacactgaac ttagcgcccg tgaaattgct gaaaaggcgt
     8761 tggatattgc aggcgacatt tgcatctata ccaaccattt ccacaccatc gaagaattaa
     8821 gctacaaagc gtaaggatct cccatgtctg aaatgacccc acgcgaaatc gtcagcgaac
     8881 tggataagca catcatcggc caggacaacg ccaagcgttc tgtggcgatt gctctgcgta
     8941 accgctggcg tcgcatgcag ctcaacgaag agctgcgcca tgaagtgacc ccgaaaaata
     9001 tcctgatgat cggcccgacc ggtgtcggta aaactgaaat cgcccgtcgc ctggctaagc
     9061 tggcgaatgc gccgttcatc aaagttgaag cgaccaaatt caccgaagtg ggctatgtcg
     9121 gtaaggaagt ggattctatt attcgcgatc tgaccgatgc cgccgtgaaa atggtacgcg
     9181 tccaggctat cgagaaaaac cgttatcgcg ctgaagaact ggcagaagaa cgtattctcg
     9241 acgtgctgat cccacctgct aaaaacaact ggggacagac cgaacagcag caggaaccgt
     9301 ccgctgctcg tcaggcattc cgcaaaaaac tgcgtgaagg ccagcttgat gacaaagaaa
     9361 tcgagatcga tcttgccgca gcaccgatgg gcgttgaaat tatggctcct ccgggcatgg
     9421 aagagatgac cagccagctg cagtccatgt tccagaacct gggcggtcag aagcaaaaag
     9481 cgcgtaagct gaaaatcaaa gacgccatga agctgctgat tgaagaagaa gcggcgaaac
     9541 tggtgaaccc ggaagagctg aagcaagatg ctatcgacgc tgttgagcag cacgggatcg
     9601 tgtttatcga cgaaatcgac aaaatctgta agcgcggcga gtcttccggt ccggatgttt
     9661 ctcgtgaagg cgttcagcgt gacctgctgc cgctggtaga aggttgcact gtttccacca
     9721 aacatgggat ggtcaaaact gaccacattc tgtttatcgc ttctggcgcg ttccagattg
     9781 cgaaaccgtc tgacctgatc ccggaactgc aaggtcgtct gccaatccgc gttgagctgc
     9841 aggcactgac caccagcgac ttcgagcgta ttctgaccga gccgaatgcc tctatcactg
     9901 tgcagtacaa agcactgatg gcaactgaag gcgtaaatat cgagtttacc gactccggta
     9961 ttaagcgcat cgcggaagcg gcatggcagg tgaacgaatc taccgaaaac atcggtgctc
    10021 gtcgtttaca taccgttctg gagcgtttaa tggaagagat ttcctacgac gccagcgatt
    10081 taagcggtca aactatcact attgacgcag attatgtgag caaacatctg gatgcgttgg
    10141 tggcagatga agatctgagc cgttttatcc tataatcgcg ttcaatcatt ttcatcattg
    10201 tttgatgggg ctgaaaggcc ccatttttat tggcgcgtat tatgactgaa caacaaatta
    10261 gccgaactca ggcgtggctg gaaagtttac gacctaaaac cctccccctc gcctttgctg
    10321 caattatcgt cgggacggcg ctggcatggt ggcaaggtca cttcgatcca ctggtcgccc
    10381 tgctggcatt gattaccgcc gggctattac agatcctttc taacctcgcc aatgattacg
    10441 gcgatgcggt aaaaggcagc gataaacctg accgcattgg gccgctacgc ggcatgcaaa
    10501 aaggagtcat tacccagcaa gagatgaaac gggcgctcat tattaccgtt gtgctcatct
    10561 gtctttccgg gctggcactg gttgcagtgg cgtgccatac gctggccgat tttgtcggtt
    10621 tcctgattct tggcgggttg tcgatcattg ccgctatcac ctacaccgtg ggcaatcgtc
    10681 cttatggtta tatcggtttg ggtgatattt ccgtactggt tttctttggc tggttgagcg
    10741 tcatggggag ctggtattta caggctcata cattgattcc agcactgatc cttccggcaa
    10801 ccgcatgcgg cctgctggca acggcagtac tgaacattaa taacctgcgt gatatcaata
    10861 gcgaccgcga aaacggcaaa aacacgctgg tggtgcgctt aggtgcagta aacgcgcgtc
    10921 gttatcatgc ctgcctgctg atgggctcac tggtgtgtct ggcgctgttt aatctctttt
    10981 cgctgcatag cctgtggggc tggctgttcc tgctggcggc accgttgctg gtgaagcaag
    11041 ctcgttatgt gatgcgcgaa atggacccgg tggcgatgcg accaatgctg gaacgcactg
    11101 tcaaaggagc gttactgact aacctgctgt ttgttttagg gatattccta agccagtggg
    11161 cagcataact gacaaatatc aattaacaat tgatgatttt gccaacagcc cacatagcgc
    11221 gatatactga aaattctcgc agcaactgaa tgttaagcct atgaaatacg atacttccga
    11281 gctttgtgac atctatcaag aagatgttaa cgtcgtggaa ccgctgttct ccaactttgg
    11341 cggacgggcg tcgtttggcg gacaaataat cacggtaaaa tgtttcgagg acaacgggtt
    11401 gctgtacgat ctgctcgaac agaatggccg tggtcgtgtt cttgtcgtcg atggcggagg
    11461 ttctgttcgt cgcgcactgg tcgatgctga actggcgcgt ctggcagtac aaaatgaatg
    11521 ggaaggtctg gtcatttacg gcgcggtgcg tcaggtagat gacctggaag agttggatat
    11581 cggcatccag gcgatggcgg caattccggt tggtgccgct ggcgtaggca ttggcgaaag
    11641 cggtgtccgc gtcaattttg gcggtgtcac cttcttctcc ggcgaccatc tttatgccga
    11701 caataccggg attattcttt cagaagatcc gctggatatt gaatgataaa aaaggcaccg
    11761 caaggtgcct tttttctgcg ttacctgttg gcctacacag taaagaaatt acgcaaaaga
    11821 tgaagtgtaa tcagacctct tccatgcgac ccagcagggc ctgtagacgt tcctgccagc
    11881 cgttctgctg ttctttcaga tggttgttct cacgctccag ctcttcgcgc tgatgctggg
    11941 cattttgaac ttcctgcgac agtgagttgt ttttttcttt cagctcttcg atttccatct
    12001 gcaacagagt gatggtatca atcgcctgct gtacttttgc ttccagtttc tcaaacactt
    12061 ctaatgacat tgtcatacct ctcctgaatt gcaaggcgtt gatggataaa aatcctcgtc
    12121 ccgattaccg gtgacgcctt aataaatacg agcgcacttt agttagctcc gattgtatga
    12181 agccgcgcca tcgctgtcca gcggcacgcc ttgcagatta cggtttgcca cacttttcat
    12241 ccttctcctg gtgacataat ccacaccaat cgaaaatgtt aataaatttg ttgcgcgaat
    12301 gttctaacaa acatgcatca tgtacaatca gatggaataa atggcgcgat aacgctcatt
    12361 ttatgacgaa gcacacacat tttaagttcg atatttctcg tttttgctcg ttaacgataa
    12421 gtttacagca tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat
    12481 taactcttca ggatccgatt atgagtcaaa catcaacctt gaaaggccag tgcattgctg
    12541 aattcctcgg taccgggttg ttgatttttt tcggtgtggg ttgcgttgca gcactaaaag
    12601 tcgctggtgc gtcttttggt cagtgggaaa tcagtgtcat ttggggactg ggggtggcaa
    12661 tggccatcta cctgaccgca ggggtttccg gcgcgcatct taatcccgct gttaccattg
    12721 cattgtggct gtttgcctgt ttcgacaagc gcaaagttat tccttttatc gtttcacaag
    12781 ttgccggcgc tttctgtgct gcggctttag tttacgggct ttactacaat ttatttttcg
    12841 acttcgagca gactcatcac attgttcgcg gcagcgttga aagtgttgat ctggctggca
    12901 ctttctctac ttaccctaat cctcatatca attttgtgca ggctttcgca gttgagatgg
    12961 tgattaccgc tattctgatg gggctgatcc tggcgttaac ggacgatggc aacggtgtac
    13021 cacgcggccc tttggctccc ttgctgattg gtctactgat tgcggtcatt ggcgcatcta
    13081 tgggcccatt gacgggcttt gccatgaacc cagcgcgtga cttcggtccg aaagtctttg
    13141 cctggctggc gggctggggc aatgtcgcct ttaccggcgg cagagacatt ccttacttcc
    13201 tggtgccgct ttttggccct atcgttggcg cgattgtagg tgcatttgcc tatcgcaaac
    13261 tgattggtcg ccatttgcct tgcgatatct gtgttgtgga agaaaaggag accacaactc
    13321 cttcagaaca aaaagcttcg ctgtaatatg actacgggac aattaaacat gactgaaaaa
    13381 aaatatatcg ttgcgctcga ccagggcacc accagctccc gcgcggtcgt aatggatcac
    13441 gatgccaata tcattagcgt gtcgcagcgc gaatttgagc aaatctaccc aaaaccaggc
    13501 tgggtagaac acgacccaat ggaaatctgg gccacccaaa gctccacgct ggtagaagtg
    13561 ctggcgaaag ccgatatcag ttccgatcaa attgcagcta tcggtattac gaaccagcgt
    13621 gaaaccacta ttgtctggga aaaagaaacc ggcaagccta tctataacgc cattgtctgg
    13681 cagtgccgtc gtaccgccga gatctgcgaa catctcaaac gcgatggcat ggaagagtac
    13741 attcgtaaca acaccggtct ggtgattgac ccgtacttct ctggcaccaa agtgaagtgg
    13801 atcctcgacc atgtggaagg ctctcgcgag cgtgcgcgtc gtggtgaatt gctgtttggt
    13861 acggttgata cgtggcttat ctggaaaatg actcagggcc gtgtccatgt gaccgattac
    13921 accaacgcct ctcgtaccat gttgtttaac atccatagtc tggactggga cgacaaaatg
    13981 ctggaggtgc tggatattcc acgcgagatg ctgccagaag tgcgtcgttc ttccgaagta
    14041 tacggtcaga ctaacattgg cggcaaaggc ggcacgcgta ttccaatctc cgggatcgcc
    14101 ggtgaccagc aggcggcgct gtttggtcag ttgtgcgtga aagaaggaat ggcgaagaac
    14161 actggcgaga aagcggtaaa atcagaaaac ggcctgctga ccaccatcgc ctgcggcccg
    14221 actggcgaag tgaactatgc gctggaaggt gcagtgttta tggcgggcgc gtccattcag
    14281 tggctgcgcg atgagatgaa gttgattaac gacgcctacg attccgaata tttcgccacc
    14341 aaagtgcaaa acaccaatgg tgtgtatgtg gttccggcct ttaccgggct gggtgcgcca
    14401 tactgggatc cgtatgcacg cggggcgatt ttcggtctga ctcgtggggt gaacgctaac
    14461 cacattatac gcgcgacgct ggagtctatt gcttatcaga cgcgtgacgt gctggaagca
    14521 atgcaggccg actctggtat tcgactgcac gccctgcgcg tggatggcgg cgcagtagca
    14581 aacaatttcc tgatgcagtt ccagtccgat attctcggta cgcgcgttga gcgcccggaa
    14641 gtgcgcgaag tcaccgcttt gggtgcggcc tatcttgctg gtctggcggt tggcttctgg
    14701 cagaatcttg acgagctgca agagaaagcg gtgattgaac gcgagttccg tcctggcatc
    14761 gaaaccactg agcgtaatta ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg
    14821 gcatgggaag aacacgacga gtaataatga ttgcctgatg cgacgcttgc tgaactgaac
    14881 cccaaaacct ggacagtttc agttaaccgg ctttttgata ctca
//
LOCUS       DAWDRC010000023        14836 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2128_length_14836_cov_2.096137, whole genome shotgun sequence.
ACCESSION   DAWDRC010000023 DAWDRC010000000
VERSION     DAWDRC010000023.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14836)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14836)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2128_length_14836_cov_2.096137"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    554..563
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1976..1985
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4366..4375
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7562..7571
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10496..10505
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3718 a         3618 c         3850 g         3600 t
ORIGIN      
        1 caaataccga tgtcatcttt accgttgatg gtttcagttt tagcggcagc ggacagaaca
       61 ccgggatggc gtttgtttcg ttgaaaaact ggtctcaacg taaaggggca gaaaacaccg
      121 cccaggctat cgccctacgg gcaaccaaag agctgggcac aattcgtgat gccacggtat
      181 ttgcgatgac gccgccagcc gttgatgggc tggggcaaag caatggtttt acgtttgaat
      241 tgttagctaa cggtggaacc gatcgtgaaa cactgctgca aatgcgtaat cagttgatag
      301 aaaaagcgaa tcaaagtccg gagttacatt ctgtacgcgc caatgatttg ccacaaatgc
      361 cgcaattgca ggtagatatt gatagcaata aagcggtgtc attagggttg agtttgaatg
      421 atgtcaccga caccctgtcc agtgcgtggg gcggtactta tgtgaatgac tttattgacc
      481 gtggtcgtgt gaaaaaagtc tacattcagg gcgacagcga atttcgttcc gcgccgtcag
      541 acttaggtaa atgnnnnnnn nnnacggctc ggcagcctat gaaattcagg gcgaaaacgc
      601 gactggcttt agttccggcg atgcaatgac aaaaatggag gaactggcaa acagtcttcc
      661 tgcgggaaca acctgggcct ggagtggttt gtcattgcag gagaaactgg ccagcggtca
      721 ggcattaagt ctgtatgcag tttctattct ggtggttttc ctctgccttg cagcactgta
      781 cgaaagctgg tcagtcccgt tctcggtcat tctggtgatc cctctggggc ttcttggcgc
      841 ggcgctggcg gcctggatgc gtgatttaaa caacgacgtt tacttccagg tagcgctatt
      901 aactactatc ggcttgtcgt cgaaaaacgc cattctgatt gtggaatttg ctgaagcggc
      961 ggttgcagag ggctattcct tgagtcgtgc ggcattacgc gcggcgcaga ctcgtttacg
     1021 cccaatcatc atgacctcgc tggcgtttat tgcgggggta atgccgctgg cgatagcaac
     1081 cggcgcaggg gcgaacagcc gcatcgccat tggtacgggc attattggcg gcacgctgac
     1141 cgctacatta ctggccattt tcttcgttcc tctgtttttt gtactggtga aacgtttgtt
     1201 tgccggtaaa ccgcgccgtc aggagtaagt tatgttgcgt cgttctctta tttttctggt
     1261 gttgttgagt gcgggatgtg tctcgctcga tcctcactac agtacgcctg aatccccaat
     1321 cccggcgacc ttgccgggag cacagggcca ggggaaggca atcagccatg actggcagca
     1381 ggtgatccac gatccccgat tgcagcaggt agtaaccatt gcactgaaca gtaaccgcga
     1441 tgtgcaaaaa gcgattgctg atattgactc agcacgggcg ctctatgggc aaactaatgc
     1501 gtcgttattt ccgacggtga atgcggcctt aagttcgact cgcagtcgtt cgctggcgaa
     1561 tggtacggga acaactgccg aggctgacgg cacggtgtcc agttatacgt tagatctgtt
     1621 tggtcgtaat cagagtttat ctcgggcggc gcgggaaacc tggcttgcca gtgaatttac
     1681 agcccagaac actcgtttaa cgctgattgc tgaaatcagc accgcctggc tgacactggc
     1741 ggctgataac agcaacctgg ccctggcaaa agaaacgatg accagtgcag aaaactcatt
     1801 gaaaattatc cagcgccagc aacaggttgg cacggcggca gcgacagatg tcagtgaggc
     1861 gatgagtgtt tatcagcaag cgcgcgccag cgttgccagc tatcagacac aagtgatgca
     1921 ggataaaaat gcgctgaact tactggcagg tacaacgctt gcggaaaatc tgctannnnn
     1981 nnnnnctgga aagcctaccg gaacaaatga taagtctggt tcctgcggga gtatcatctg
     2041 atgttctgct gcgtcgcccg gatatccagg aagcggaaca taatttgaag agcgccaatg
     2101 cagatattgg cgcggcgcgg gctaactttt tcccgacgat ttcgctgact gccagtgccg
     2161 gtgttggcag tgacgcattg tcgtcgttgt tcagccacgg aatgcagatc tggtcatttg
     2221 cgccatcggt cacattaccg ctgttcactg gcggaagtaa tctggcgcag cttcgttacg
     2281 cagaagcaca aaagcgtggg ctcattgcca cctatgaaaa aaccgttcag agcgcattta
     2341 aagatgtcgc taacgcactg gcgcggcgga ccacgcttga agagcaactg gatgcacagc
     2401 gccagtatgt taaagctgag caacaaacgg tcgatgttgg cttgcgtcgc tatcaggcgg
     2461 gtgttggtga ttacctgacc gtgttgacgg cgcagcgcag cttgtggagc gcacagcagg
     2521 agctgctggc actgcaactg actgatttta caaaccgaat cacgctctgg cagtcgctgg
     2581 gcggcggtat gtcatcactc aagtaaggaa taatatggct agagtctctc tttcatgggc
     2641 attgattctt ggtcttttag ccggtatcgg cccgatgtgt accgatcttt atttgccggc
     2701 tttgccggag atgtctgagc aactggcggc aaccacgacc ataacgcaat taaccctgac
     2761 cgcatcactg attggtcttg gcgtcggaca actgttattt ggccctctga gcgacaaaat
     2821 agggcgtaaa cgtccgttaa tcttgtcgtt gttattgttt attgtttctt ccattttgtg
     2881 cgcgacaacg aacaatattt actggctggt ggtctggcgt tttattcaag ggatcgcggg
     2941 ggcgggtggt tcggtgctct ctcgttctat tgctcgtgac aaatatcagg gagtaacgtt
     3001 gacccagttt tttgcgctgt taatgacggt gaatggcctg gcaccggtgt tgtcgccagt
     3061 gctgggcggg tacattgtta gcacttttga ctggcgtact ttattctggg taatggctga
     3121 aattagcacc gtactgttgc tgggctgtct gttatttatt catgagacct tgccagaaaa
     3181 taaaaggggc tcatcattgc tattaaccgg acgaagcgtg gtgcagaacc gccgttttat
     3241 gcgcttttgc ctgattcaaa gttttatgct ggctggtttg tttgcatata tcggctcttc
     3301 gtcgttcgtg ttgcagaagg aatttggctt tagtccaatg caatttagcc tggtgtttgg
     3361 ccttaacggc atcggactta tcattgcttc atggatcttc tcgcgcctgg cgcgacggat
     3421 taacgcgatg acattgttgc gaggtggcct gatagcggca attttgtgtg cattgctcac
     3481 ggtcttatgc gcatgggtac aattgcccat tccggcactg gtggcattat ttttcaccat
     3541 cgcattttgt agcggcatcg gcactgttgg cggggcagag gctatgagtg cagtagggac
     3601 gcaggaatct ggaacggcgt ctgcgttgat ggggatgagc atgtttgtct tcggcggtat
     3661 agccgcgcca ttgtcgggaa ttggcggaga aacactgtta aaaatgagtc tggcaattac
     3721 ggtgtgttat acgctggcat tgctggttgc tctcaccaga atcgacaatc aaaagtaatg
     3781 aacacctcat cctgaggggg gcctgtcagg atgaggtgca aaacactaac ggtccttacg
     3841 ccacgcatcc gccgtcaatg cttcgccaaa atgaccggcg atcagccgtt tggtgagttc
     3901 atgaagcggc gatgccagca catctgcggt gctgcctcgc tcgacaacct cgccctgatg
     3961 catcaccagc acctggtcgc taatgtgctt catcattccg atatgctggg taacataaat
     4021 atacgaaatg ccctgttttt cctgtaactc cagcatcaga ttaatcaact gcgaacgcat
     4081 cgacatatcc agtgaggcga gggcttcatc ggcaataatg acttttgggc gcaatatcag
     4141 cgcgcgcgcc agacccagac gctgtttttg cccgggtgct aacatatgcg gatagtaact
     4201 gacgtgatcc ggtagcagcc caaccatacg catcgtttca ataatctgtt tgcgacgctg
     4261 ttccggttcc aggtcggtgt tcaggcgcag tggaaaatcc agaatttgcg agatacgttg
     4321 acggggattc aacgaggtcg aaggatcctg aaaaatcatg cgaatnnnnn nnnnnctcgc
     4381 cgctggtcgc ttctatcatt cccgacagca tttttgccag cgtggattta cccgaaccat
     4441 tctcgccgat aatcgccagt gtctggcctt cacgtagcgt aaagctcaag ggttttaccg
     4501 cttctacggt ctgacgacga aaccagccgg tccggtagcg gaacgtttta cttaaattgc
     4561 gcacttcaag tagcgtttcg atcatctcac tctttctcca tgttcagcgg gaaatgacag
     4621 gcatagagat gatttttcgc ccccgtcaaa cgtggcgtca caatgcattc tcgttgtgca
     4681 tacgggcaac gtggccccag acgacaacca atcggtaact gttccagtag cgggatagcg
     4741 ccgggcagcg tattgaggcg acttttatgc ggcatcgcgc tgccgaagtc tggtatcgcg
     4801 cggatcagcg cctgggtata aggatggtgt ggcatcgtca ccaactcctt actcggcgcg
     4861 gtttccaccg tttgaccgca gtaaagcacg ttaattttat ccgcccattg gctaagcatt
     4921 tgtaagtcat ggctgataag caaaatagtg gtattactgt tttggttgag acgcgtcagc
     4981 aggcgaaaga tttgcgcctg ggttgttggc tccattgagt tggtcggttc gtcagcaatt
     5041 agcagacgcg gttgattcgc cagtgcaatg gctatcatca ctttctgaca ttcaccttcg
     5101 gtcaactcat agggaaaact gcgcatcgca tctttgtgat ctttaatccc cacgcggtgc
     5161 agcagttcaa tggcacggcg tttgcgccag ccaaaacgct gccaccaacg gcctttatag
     5221 gtccaggcag ggatgttttg catcaactgg cgacccacac gttctgaagg gtcaagacac
     5281 gactgtggtt cctggaaaat catcgacacg ttgtgaccaa ccagcttgcg ccgttcgcgt
     5341 gcggagagac gcagcaaatc gatatcatca aaacgcatac ggtcagcagt aacacgccag
     5401 ttatctttat tcaccccaca aatcgcttta gcaatcaaac ttttgccgga accggattcg
     5461 ccaacaagac cgcggatttc accttcggtt aaggtcatac ttacgcggtc gacggcttta
     5521 acccactcat caccggtttt aaattcaatg gtcaggttac gaatatcgag taatggcatt
     5581 attccacccc cgcaataatt gcacgacgga caccgtcgcc aaggaggtta accaacagca
     5641 cgctaatcat aattgccgca cctggcagca tgacagtcca tggggcgaca taaatcagtt
     5701 ccagcgcatc accgagcatc gctccccatt caggcgaggg cagttgtgcg ccgaggtcaa
     5761 gaaagcccag cgcggcgata tcgagaattg ccatcgacaa tgcgcgagtg atctcggtga
     5821 ccagtccggc ggtgatgttg ggcatcacag caaaccagag aatattcagc gttgatgcgc
     5881 catccagacg ggcggcgata acgtactctt tttccagttc gtcatgcacc atgctgtaaa
     5941 tcgaacgtac catacgtggc agcagcgcca gccagacagc aaacatggcg tgagacaaac
     6001 tcggtccggc aaacgctaca acgataattg ccagcagcag cgaagggatc gccagtaagg
     6061 tatccagaat atggttaagc accgctgagc gtaggccgtg ggtcgccccg gcgaaggtgc
     6121 ccagtaccag gccacagatc gtcgcggcaa gcgtcaccac aaatgcgcca cctacggttg
     6181 gcgcagctcc gctcagtaaa cggcttaaaa catcgcgtcc gaggtcgtca gtccccagga
     6241 agaaagaaac ttcgccatag cgcgaccatg acggcggcaa taattgataa ccgagaaatt
     6301 gctggtcgat gccgtaaggc gcaaaccagc cgccaaaaat acacagtaca gccagccccg
     6361 cgcagccgta caggccgacc atcgcagagg cgtcactata aaatttgcgc caggcggtac
     6421 gcagcgtgcc cggcgggcgc ttttcgctgt atacgctatc gtaaggcata ccattcctta
     6481 tgtttcagag ggttagccat ggcacccaaa atatcagaaa tcacgttaac aataatgacc
     6541 agtgagccac acaccatcac tccggcggaa atggctgcgt aatcctgctg gcggatggcg
     6601 ttaattaacc agcgccccaa acccggccag ctaaaaacca tttcggtgat catcgccagc
     6661 gtcagcatgg tggaaaactg taagcccagg cgaggaataa ccggaggtaa cgcgttatgc
     6721 agaacgtggc gacgcaaaat agtaaagcgc gacaaaccgc gggttgccgc tgctttaaca
     6781 tagttctggt catacacttc gatagtgctg atacgcatca gtcggatcac ttcagttgtt
     6841 ggcgcaaccg acagggtgat cacgggcaat atcatatggc ggattgcgct catgaccatt
     6901 tcatcccgcc acggtgagtt cgaaagccag gcatcaatca acgcaaaacc ggtaatcggt
     6961 ttcacttcgt agagcaaatc gaaacgccca gaaacgggca accaacctag tgtgagtgaa
     7021 caaaacagcg tcagcagaag cgccagccag aacaccggga ttgaaaagcc cagtaaggcg
     7081 atggcgttga tcaagttatc ctgccactta tggcgcgtaa tcccggcaat cattcccacg
     7141 gggatgccaa caatcagcgc aaaaccgaag gcgaggatgc acagttccat cgtcgccggg
     7201 aagacctctt ttaactgttc ggcaatcggt tgaccgttaa tgctggaaac accaaaatcc
     7261 cagtggatca ggccgttaaa ccagaacacc caggcattcc acagtgacgc gccttgtaac
     7321 ggcgcatgtg gggtgaaata gctcaagctg aagccaacga aggtcagcag gaacaaggtc
     7381 acaatcaata acaaaatacg gcgtaaggtg aagataatca tggctttttc acctcatcct
     7441 gtttctcgcg atacacccca gcaaaggagg cgttaccaaa cgggctaagt accagacctt
     7501 tgatatcgta ccgataggcc tgcaaacgca atgacgacgc cagcggcaga ataggtaatt
     7561 cnnnnnnnnn ncaccagtgg gcgaggttgg tctgcgaatg aattgctgcg cagctcagta
     7621 acggacggaa gaaactgtcc gggtcgttac tgtccgtcgc ccaaccggat aacgtcagat
     7681 catggctcat atccatcaac cgcgcctcct gaaaacgacc ttctaccggc acaatcacca
     7741 cttttacgcc aacctgtgcc atatccgcct gaatcagttc ggcggttttc agtggactgg
     7801 ggttccacgc ctgcgaacgt gtgggcaccc acagtttcag cgttaagttt tccagcccca
     7861 acgccttcaa ctgttcgcgc gattttgccg gattgtattc agtaatttta gcctcgttgt
     7921 cataggccca cgaggctcgc ggtaaaatag aggcagctgt ttcagccgta ccataataga
     7981 tggattgcat caggcgctgg ttattaatcg ccagtgccag cgcatggcgg accgccggat
     8041 tatttagcgg cggtttggcg gtgttgaatg ccagataggc gacattcatc ccaggacgca
     8101 gcgttaaacg caagcgtggg tcgtcacgca aaatggatag ctgactggca gcaggccagg
     8161 ccagaacgtc gcactccccg gtcaggagtt tcgacaatcg tcctgtaccg ccagagccta
     8221 aatctaccac cacttgtggc atcaacggct taccacgcca gaaatcatca tgacgttgta
     8281 agcgaatata ttgcccggcg cggtattccg acaactgata cggaccggtg ccgaccggtt
     8341 gacggtcgag ttgctcctgg cgatcttctt tctctaactt ccctgcatat tctgccgaca
     8401 tgaccgaagc ataatgggtc gccaggtgcc acaaaaaaga agcatccggc tgggcgagac
     8461 ggaactcaac ggtatgatta tccagcttgc ggacgctttt cacgttatcg gcaaattgca
     8521 gactgtcgaa ataggggaag ttactgccgt tgacgttatg ccacgggttg ttgcggtcaa
     8581 aaatccgttg gaaggtaaac accacatcat cggcattcat tttacgagtg ggagtaaacc
     8641 agtcggtttt ttgaaacgga acatcgcgac gcaggtggaa gcgataggtc gcgccgttgt
     8701 cgagtacttc ccagctttcg gcaagttccg gcatcaggcg ataggtatag ggatcgacat
     8761 ccagcagtcg atcataaaac tgggcggcaa gggtatcgac aattaaccca ctgctcgctt
     8821 tggatgggtt aaaggtgttg acttgcccgc tgacgcaata gacaaaaccg ctgtcgcgga
     8881 tatcagcatg cgggggggat tcaggcgcgg cgattgcctg accactcaca agtccagcaa
     8941 tcaccaaaag agacgataat acctggcgca taatattaag ggattttatg taaagaggct
     9001 atcttactaa tatttaatga catttgccat taccgtttgt gttcagaggt cgtaatgagg
     9061 gccgtgtggt tggtcattga acaggtggcg tcgccatcag ttcaacattc gtacccgtga
     9121 tttctttata actataactc acagaacaac ttagcgagga gcagagcggt acgatcgtga
     9181 gaaacccatt aaagcacgct gctgtgcgtg tcatttttag ccggatgcgg cgcgaacgct
     9241 ttatccgggc aacgatactg accgatcgtc tgcaattaaa taattactca ttaccccatt
     9301 gattcaaaaa ctctgcgatc tcatcaatgc gtacgggatt aatccccgct tcagcagcca
     9361 tttcatgttg ggcttcttca ctgatctctt cattgttcat caaacgggtg agcagtaact
     9421 ggaagtagtg ggccagtggt gtgcgttcag cgtctgcgac aggctctgcg cattcggtcg
     9481 cgtactcatc ggcaatatca aaatatttta gcgggatatc gtggttcatt atttgcccct
     9541 ggggttaatg cgctaagcat aaaggcggat gatagcactc gtgatccggg tcatacatat
     9601 ttttatagtt aatggcgcgg tgcattaccc tcaggcagga taatgcgccg cgcattcgac
     9661 tattacgttt cgctcaccgg cgttctgtcg ccatcataca acggcacttt gcgatagcgg
     9721 cggatcaaat accacaaata tgcgccgcct aacgaagccc ataccatccc ggttgcgcaa
     9781 ttcgatgcgg caatgaaaat tttgcaggat gcgctgagag attaatacgc agaagcaggg
     9841 ttaacgcaat aatgcctgat gcgcagcgct tatcaggcct gcaaacgacg tattgattat
     9901 gtatgccgga taaagcgttc acgctgcatc cggcaagttg tattgcccaa cttcgctaaa
     9961 tctggtgctt tttcaacaac gcgcggaact gatgataggt taaccccagc aattcagcgg
    10021 cgcgcttctg gttatatttc ccctgttgca aactgagttg cagcaactct ttttcctgct
    10081 gcatctgaaa ctcgcgtaaa tccagtggca gcgtcggaag cgaggtgttt tctgaaacgg
    10141 cgatggcttc ttcagatgga cggcgtttaa agggatcaat aatgatgtca tcaagcggat
    10201 aatcgctggt gccgtgacga tacactgaac gttccaccac gtttttcaat tcacgtatat
    10261 ttcccggcca gcgataattc agcaatgttt ctctggcgtg ctcggtaaaa cccgggaaca
    10321 gaggcagctt gatttcccga cacatctgga tggcaaagtg ttctgccatc aacataatgt
    10381 cgctttcacg ctcgcgcagt ggtggcagtt gcacaacatc gaacgctagt cggtcgagca
    10441 ggtcagcgcg aaaagtacct tcattgacca tcgccgggag atcggcattc gtcgcnnnnn
    10501 nnnnntgcaa tggctggctg ccgccgacgc gctccagttc accgtactca atcacacgca
    10561 ataatttttc ctgcaccatc atcggtgccg tagcgagttc atcaagaaat agcgtaccgc
    10621 cgtcggcacg ttcaaatctc cctggatgac gtttttgcgc accggtaaac gcccccgctt
    10681 catgaccaaa cagttcggaa tccagcaaat tttcatttaa cgccgcgcag ttaagggaaa
    10741 taaacggccc ttgccaacgg gaggagagat aatgcaggcg gctggcgatc agctctttac
    10801 cggtgccgcg ctcgccgatg atgagcaccg gtttgtccag cggtgcgaga tgcgaaacct
    10861 gttccagcac ttcgagaaag ctgttcgcct caccgagtaa attatctttg tattctgcca
    10921 tgatgaaatt cgccacttgt tagtgtaatt cgctaattca tcctggcatg ttgctgttga
    10981 ctcttcaatc agatctttat aaatcaaaaa gataaaaatt ggcacgcaaa ttgtattaac
    11041 agttcagcag gacaatcctg aacgcagaaa tcaagaggac aacattatgg gtattttttc
    11101 tcgctttgcc gacatcgtga atgccaacat caacgctctg ttagagaaag cggaagatcc
    11161 acagaaactg gtgcgtctga tgatccagga gatggaagat acattggttg aagtacgttc
    11221 tacttcggcg cgtgcgctgg cagaaaagaa acagctgact cgtcgtattg aacaagcgtc
    11281 ggcgcgtgaa attgaatggc aggaaaaagc ggaactggca ctgctgaaag atagagaaga
    11341 tctggcgcgt gcggcgttaa ttgaaaaaca gaaactgacc gatctgatta agagcctgga
    11401 acatgaagtg acgctggtgg acgatacgct agtgcgtatg aagaaagaga tcggcgagct
    11461 ggaaaacaaa ttgagtgaaa cacgcgctcg ccagcaggca ttgatgttac gccatcaggc
    11521 ggcaaactcg tcgcgcgatg tgcgtcgtca gctggacagt ggcaaactgg atgaagcaat
    11581 ggctcgtttc gaatctttcg aacgtcgaat tgaccagatg gaagcggaag cagaaagcca
    11641 cagcttcggt aaacaaaaat cgctggacga tcagtttgcc gaactgaaag ccgatgatgc
    11701 aatcagcgag caactagcac aattaaaagc caaaatgaag caagacaatc aataataaca
    11761 tccaggcggc gtccgaacgc gccgccgctc atcgtctaag gagtccttat gagcgcgcta
    11821 tttctggcta ttccgttaac catttttgtg ctgtttgttt tacctatctg gttatggctg
    11881 cattacagca atcgttctgg tcgcagtgaa ttgtcgcaaa gtgagcagca gcgattagcg
    11941 caactggctg atgaagcaaa acggatgcgc gaacgtattc aggcgctgga atctattctt
    12001 gatgcagaac atccgaactg gagggatcgc taatggcggg cattaatctc aataaaaaat
    12061 tatggcgtat tccgcagcag ggcatggtcc gtggcgtctg cgccgggatt gccaactatt
    12121 ttgatgtacc agtaaaactg gtgcgtatcc tggtggtgct gtcgattttc ttcggtctgg
    12181 cgctgtttac cctggttgct tacatcattt tgtcatttgc gcttgatcca atgccggaca
    12241 acatggcctt tggtgagcag ctaccttcca gccgcgaatt gctggatgaa gtcgaccgtg
    12301 aactggcggc aagtgaaacg cgtttacgcg agatggaacg ttatgtcact tccgatactt
    12361 tcacgttacg tagccgtttc cgtcaactgt gaggaaagtt atgaatactc gctggcaaca
    12421 ggccgggcaa aaggtgaagc ctgggttcaa attagcaggc aagctggtac ttcttaccgc
    12481 actgcgctat ggcccggcgg gtgtggcggg ctgggcgata aaatcagttg ctcgccgacc
    12541 gctgaaaatg ttgctggctg tggcgctgga accgctgtta agtcgggctg ccaataaact
    12601 ggcgcagcgt tataaaaggt gaggggagtt tcgcaaaaat ggttaatctc aggcgtataa
    12661 tggatagcaa ttttcatcca tagaaggacg cttacatgtt taaaaaaggc ttacttgctc
    12721 tgacactggt gttttcactg cccgttttcg ccgctgaaca ctggatcgat gttcgtgttc
    12781 cagagcagta tcagcaagaa cacgttcagg gggccatcaa tattcccttg aaggaagtga
    12841 aagagcgcat tgccaccgcc gttccggata aaaacgacac cgttaaagtg tattgcaatg
    12901 ccggacgcca gtcagggcag gcaaaagaga tccttagcga gatgggatat acccacgttg
    12961 agaacgccgg tggcctgaaa gacatcgcaa tgccgaaggt caaaggttaa aagaattcac
    13021 catgagcggc gcttatgcgc cgttttttct gtcacaccct atttacatcc ccatagatta
    13081 tttgcgttag ctcacaaata cgctttttcc caggtaaaaa aagatttcct gcgtgactaa
    13141 accccttgag ctcaattgac agtttatttt atgcggagta gtctctcgtt tcatgggacc
    13201 gctaccacgg aaaggcaaca tgaaacagaa aattacggat tacctggacg aaatctacgg
    13261 tggaacattt accgcgactc atttacagaa acttgtaacg cgtcttgaga gtgcgaaacg
    13321 attaattaca cagcgacgta aaaaacactg ggatgaaagt gatgtcgtgt taattaccta
    13381 tgccgatcaa tttcacagta atgatttaaa gccactaccc acatttaatc agttttaccc
    13441 tcaatggctg caaagaattt tttcacatgt tcatttatta cctttttatc catggtcatc
    13501 tgatgatgga ttttcggtaa ttgattatca tcaggtcgcc agtgaagcgg gggagtggca
    13561 ggatattcag caactcggtg aatgcagtca tttaatgttt gattttgtct gcaaccatat
    13621 gtcggcaaaa agtgaatggt ttaaaaacta tttacaacag cagccaggtt ttgaagattt
    13681 ttttattgcc gttgatccga aaaccgatct cagcgccgtc actcgcccgc gtgcgttacc
    13741 gttattaacg ccattccaga tgcgcgatca ttcaatgcgc catttatgga ccacctttag
    13801 cgacgatcaa attgacctca attaccgtag ccctgaagtg ttgctggcga tggtggattt
    13861 attgctgtgc tacctggaaa agggcgcaga gtatgtccgt ctggatgccg ttggctttat
    13921 gtggaaagag ccgggaacaa actgcatcca tctggaaaaa acacatctga ttatcaaact
    13981 gttacggtcg attattgata acgtcgcgcc aggcacagtg atcattaccg agaccaatgt
    14041 tccgcataaa gacaacattg cttactttgg cgaaggcgat gacgaagcgc atatggtgta
    14101 ccagttctcg ctgccgccgc tggtgctgca tgcggtgcaa aaacagaacg ttgaggcact
    14161 ttgtgcgtgg gcgcaaaacc tgacactacc ttccagcaac accacctggt ttaacttcct
    14221 tgcctctcac gatggcatcg ggcttaaccc actgcggggg ttgttgcccg aaaacgaaat
    14281 attagcgctg gtcgaggtgt tacagcagga aggagcatta gtaaactgga aaaataatcc
    14341 cgacggtacg cgtagcccct atgaaatgaa tgtcacctat atggatgcgt tgagccgccg
    14401 tgagtgtagc gacgaagaac ggtgtgccag gtttattctc gcccatgcga ttttgttaag
    14461 tttccccggt gtgcctgcga tatatattca aagtattctt ggctcgcgta atgattacgc
    14521 aggtgtcgaa aaacttggat ataaccgtgc gattaaccgt aaaaaataca ccagcgaaga
    14581 gatcacgacc gaactgaaca ataaaacgac gttaaggcac gcggtatatc atcagttgtc
    14641 gcgtttaatt acacttcgtc gcagtcataa agagtttcat ccggataata atttcaccat
    14701 tgatgcgatt aattcatttg taatgcgaat tcaaagaagc aatgcggatg gtaattgtct
    14761 gacaggattg tttaatgtca gtgaaaatat tcagcatata aatattactg acctgcacgg
    14821 tcgggatctg attagt
//
LOCUS       DAWDRC010000024        14710 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2160_length_14710_cov_2.018219, whole genome shotgun sequence.
ACCESSION   DAWDRC010000024 DAWDRC010000000
VERSION     DAWDRC010000024.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2160_length_14710_cov_2.018219"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2282..2291
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3180..3189
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3820..3829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7152..7161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8162..8171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    14009..14018
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3230 a         3748 c         4153 g         3519 t
ORIGIN      
        1 cggcattctt ggtggtatca gcagcgggca gcaaatcatt gcccacatgg cgctgaagcc
       61 aacttccagc attaccgtgc cggggcgcac cattaaccgc tttggcgaag aagttgagat
      121 gatcaccaaa ggccgtcacg atccttgtgt tgggatccgc gcggtgccga tcgcggaagc
      181 gatgctggcg atcgttttaa tggatcacct gttacggcag cgggcgcaaa atgccgatgt
      241 gaagactgat attccacgct ggtaaaaaat gaacaaaact gcgattgcgc tgctggccct
      301 gcttgccagt agtgccagcc tggcagcgac gccgtggcaa aaaataaccc aacctgtgcc
      361 gggtagcgca caatcgatag gcagtttttc taatggctgt atcgtcggcg ctgacacgtt
      421 gccgatacag tccgaacatt atcaggtcat gcgtaccgat cagcgtcgct atttcggtca
      481 cccagacctg gtgatgttta tccagcgtct gagtcgccag gtgagcaatc ttggcatggg
      541 tacggtgctg attggcgata tggggatgcc tgctggtggt cgtttcaacg gcggtcacgc
      601 cagccaccag accggactgg atgtcgatat cttcctgcaa ctgccgaaaa cacgctggac
      661 ctccgcgcag ctcttgcgtc cgcaggcact ggacctagtt tctcgtgatg gcaagcatgt
      721 cgttcctacg ctgtggaagc cagaaatttt cagcttgatc aaactcgccg cccaggacaa
      781 agacgtcacg cgcatttttg ttaatccggc gattaagcaa caactttgcc ttgatgcggg
      841 caccgatcgc gactggttgc gcaaagtgcg accctggttc cagcatcgcg cgcatatgca
      901 tgtacgatta cgttgtcctg ccgatagtct ggagtgtgag gatcaacctt taccgccacc
      961 aggcgatggt tgcggggcag aactgcaaag ctggtttgaa cctccaaaac cgggaacaac
     1021 aaagcctgag aagaagacac cgcctccgtt gccgccttcc tgccaggcgc tactggatga
     1081 gcacgtgatc taatggaaac gtttaatagc ctgtttatgg tttccccgct gctgctggga
     1141 gttctctttt ttgtcgccat gctggcggga tttatcgact cgattgccgg tggcggtggg
     1201 ttactcacca ttccggcatt gatggcggcg gggatgtctc ccgctaacgc gctggcaacc
     1261 aataaactgc aagcctgcgg tggctctatt tccgccacta tctactttat tcgccgtaaa
     1321 gtggttagtt taagcgatca aaaactcaat atcgccatga cctttgtcgg ctcaatgagc
     1381 ggcgcgctac tggtgcagta tgttcaggcc gatgtcttac ggcagatttt gcctattctg
     1441 gtgatttgta tcggcctcta tttcttgctg atgccaaagc tgggtgaaga agaccgccag
     1501 cgtcggatgt atggactgcc atttgcgctg gtggcggggg gctgcgtcgg tttttacgat
     1561 ggattctttg gcccggctgc cggatcgttt tacgcgctgg ccttcgttac gctgtgcgga
     1621 ttcaacctcg ccaaagccac tgctcacgcc aaattactta acgcaacgtc aaacattggc
     1681 ggtctgctgc tgtttattct cggcggcaaa gtgatttggg cgacgggctt tgtgatgctg
     1741 gtggggcagt tcctcggcgc gcgcatgggt tcaagactgg tgttgagcaa aggacaaaag
     1801 ctgatccgcc cgatgatcgt tatcgtctcg gcggtgatga gtgccaaatt actttacgat
     1861 agccacggac aggagatcct ccactggttg gggatgaact aatgaacagt acacaccact
     1921 atgagcagtt gattgagatt tttaatagct gctttgccga tgaatttaat acccgtctga
     1981 ttaaaggcga cgacgaaccg atctatcttc ctgctgatgc ggaagtaccg tataacagaa
     2041 tcgtcttcgc tcatggcttt tatgccagcg ccatccacga gatttcgcac tggtgtattg
     2101 ccgggaaagc gcgccgtgag ctggttgatt tcggctactg gtattgcccg gacggacgcg
     2161 atgcccagac gcaaagtcag tttgaagatg ttgaagtgaa gccgcaggcg ctggactggt
     2221 tgttctgcgt ggcggcggga tatccgttta atgttagctg cgacaatctg gaaggggatt
     2281 tnnnnnnnnn nttcgaaccg gaccgcgtgg tgtttcaacg gcgggtacat gcgcaggtga
     2341 tggattatct ggcaaacggc attcccgaac gtccggcacg cttcattaaa gcactacaaa
     2401 attattatca cacgccggaa cttacggcgg aacaattccc gtggccggaa gcgctcagct
     2461 gaagccagcg ccacgtttta caatgaggaa agaagatgat cgcggagttt gaatcacgca
     2521 ttctggcatt aatcgacggt atggttgacc atgccagtga tgatgagtta tttgccagtg
     2581 ggtatttgcg tggccacctg acattagcca tcgcagaact ggaaagtggt gacgaccact
     2641 ccgctcaggc ggtacataca accgttagcc agagtctgga aaaagccatt ggcgcgggtg
     2701 aattgtcacc gcgtgaccag gcgctggtta ccgatatgtg ggaacacctg tttcagcagg
     2761 cctcacagca gtaagttgtg acgtctggag caccatgcct gatgcgacgc ttatcgcgtc
     2821 ttatcaggcc tacaggtaac gcgtaggtcg gataaggcgt ttacgccgca tccgacaaac
     2881 aatgccaaag cagattaccc cgccttaacc gctttacctt tcaacaactt ccgcacccat
     2941 aacctattcg gatttaacgc cgccagcgta ctggcatcca tcggaatcgg ttcttcactc
     3001 atctgtgctg ccagaatctc ggcacacagc ggggcagaac acaaaccgcg cgaacctaaa
     3061 gcagcaaaca taaagagatc gtcataaacc ggcgcgctta ccgcttcatc tttcttctcc
     3121 gccagcgacg catattccac gagtgtagcg tcataatcag gcacgttgcc taccattggn
     3181 nnnnnnnnng cagatgatcg cgagtggcac aacgcacacc gcagcgcgcc tctttaccac
     3241 tgacatcaac ctcttttgcc cattgcgcgt gtgggaaaca atcaatcaac cgctggcgat
     3301 tctgctgctg atcttcatca ctgtacgccg tctcttcgct gccgcgatga taactggcac
     3361 caatacaatg gtgctgattc gccggatttt gtggcgtgag ataaccgtca tagcacagca
     3421 cctgcttcag ctccgccagt tccggcgttg tcggaatatg gctgacttga ccggccaccg
     3481 aatatactgg cagagatgac gtttggctga aacggctgat ttgatgcccg ttcgccagta
     3541 ccactacact gtgtgttgct tgctgatctc ctgcaaaatt caacaaccaa ccgtcatcct
     3601 tacgggataa atcctgtaac tgatattgat aatgaatctg caaaccctgc tgttgcgcca
     3661 gtttcagcac attacgggtc agttctgctg gacacagcca gccaccttgc ggataagtaa
     3721 tgccgctgca atttgtcgca acgcccgtaa tttgttcaac cgcatttgcc tcaacggcta
     3781 cagccagttc tgcgggtaaa tccatcgaca acatctgtgn nnnnnnnnnt tgccggaagc
     3841 accaagtgcg ggagcctcat ccgcgcaata aagcgttacc tgccagccgc gacgcaatag
     3901 cgccagtgac aacaacgcgc tggcaatacc gcctccaata attgccgctt cctgtttgtt
     3961 gctgcccgta cggttaaacc acggcgtgga gcaggggagg ggtaatgtct gttccatcac
     4021 cccacaaagc atttcccgtt tgcgcccaaa gcccttacgt ttttgcatag tgaatccggc
     4081 ttcctgcaaa ccgcggcgga caaaaccggc agacgtaaag gtcgccagcg tgctgcccgg
     4141 acgcgccagt cgggccatgg cgttaaacag attttgcgtc cacatatctg ggtttttcgc
     4201 tggcgcaaag ccgtccagaa accaggcatc tactttttga tttagcgaat cgtccagttg
     4261 gctggtcagt tcattaatat cgccaaacca taaatccagc gtcacgcggc cttcatcgag
     4321 caataaacga tggcaaccgg gcaagggcaa aggccattgc gcctgaagct gttccgccca
     4381 cggggccagc tccggccagt gttgatgcgc cagcgctaaa tccgcacggg tgaggggaaa
     4441 tttctcaaaa ctaatgaaat gtaagcgttg taattgcgct tgcggatgtg cttcgcgaaa
     4501 ctgatcaaat gcctgccata gcgtcaggaa gtttaatccg gtgccgaagc cgctctctgc
     4561 taccacaaac agaggatgtg gatgctcagg aaaacgtgcc tctaaatggt tacccccaag
     4621 aaaaacataa cgcgtctctt ccagtccgtt atcgttggaa aaatagacat cgtcaaaatc
     4681 tcgggaaaca ggtgtaccct cagcattaaa ttcgaggttg gcaggttgta tggagtagtg
     4741 tttcacgtaa gttactcgtc ttacaggcgg tggctcgatc ttagcgatgt gtgtcaggct
     4801 gcgcaaattt ctctattaat tggctgatcg gacttgttcg gcgtacaagt gtacgctatt
     4861 gtgcattcga aacttactct atgtgcgact tacagaggta ttgaatgaaa cgtgcagtga
     4921 ttactggcct gggcattgtt tccagcatcg gtaataacca gcaggaagtc ctggcatctc
     4981 tgcgtgaagg acgttcaggg atcactttct ctcaggagct gaaggattcc ggcatgcgta
     5041 gccacgtctg gggcaacgta aaactggata ccactggcct cattgaccgc aaagttgtgc
     5101 gctttatgag cgacgcatcc atttatgcat tcctttctat ggagcaggca attgcagatg
     5161 ctggcctctc cccggaagct taccagaata acccgcgcgt tggcctgatt gcaggttccg
     5221 gcggcggttc cccgcgtttt caggtgttcg gcgctgacgc aatgcgcggc ccgcgcggcc
     5281 tgaaagcggt tggcccgtat gtggtcacca aagcgatggc atccggcgtt tctgcctgcc
     5341 tcgccacgcc gtttaaaatt cacggcgtta actactccat cagctccgcg tgtgcgactt
     5401 ccgcacactg tatcggtaac gcagtagagc agatccaact gggcaaacag gacatcgtgt
     5461 ttgctggcgg cggcgaagag ctgtgctggg aaatggcttg cgaattcgac gcgatgggtg
     5521 cgctgtctac taaatacaac gacaccccgg aaaaagcctc ccgtacttac gacgctcacc
     5581 gtgacggttt cgttatcgct ggcggtggcg gtatggtagt ggttgaagag ctggaacacg
     5641 cgctggcgcg tggtgctcac atctatgctg aaatcgttgg ctacggcgca acctctgacg
     5701 gcgcagacat ggttgctccg tctggcgaag gtgcagtgcg ctgcatgaag atggcgatgc
     5761 acggtgttga taccccaatc gactacctga actctcatgg tacttcgact ccggttggcg
     5821 acgtgaaaga actggcagct atccgtgaag tgttcggcga taagagcccg gcgatttctg
     5881 caaccaaagc aatgaccggt cactcactgg gcgctgctgg cgtacaggaa gctatctact
     5941 ctctgctgat gctggaacac ggttttatcg ccccgagcat caacattgaa gagctggacg
     6001 agcaggctgc gggtctgaac atcgtgaccg aaacgaccga tcgcgaactg accacagtta
     6061 tgtctaacag cttcggcttc ggcggcacca acgccacgct ggtaatgcgc aagctgaaag
     6121 attaattcgg agtaggtcgg ataagacgcg ccagcgccgc atccgacgtt acgcgccaat
     6181 gcggcctccg gcactaacgc aaaagggaac ccgatggttc cctttttcac atcattgaca
     6241 atcgccgcca gttccaggca aacttcccgc tttgtcgatt tccttcagaa aagacgtacg
     6301 cgttaaatcc tgccaacgca ccgtaaccct gaaaccagag agatgagacg gggatactcc
     6361 tcgccttgcg ctgcattctg gagtaatgca tgactgctgt aagccaaacc gaaacacgat
     6421 cttctgccaa tttttcgctc ttccgcatcg cttttgcggt ttttctcacc tacatgaccg
     6481 taggtttacc gttgccggtt atcccgctgt ttgttcatca tgaactgggc tatggcaaca
     6541 ctatggtcgg cattgccgtc gggattcagt ttctggctac ggtgctgacg cgtggttacg
     6601 ccgggcgact ggccgatcaa tatggcgcaa aacgttcggc gcttcagggg atgttagctt
     6661 gtggtctggc ggggggcgcg ttgctgctgg cggcgatttt gcccgtcccc gcaccgttca
     6721 aatttgctct gttggtcgtc gggcgtttga ttcttggctt tggtgaaagc cagttactga
     6781 cgggcgctct gacctgggga ttaggcatcg ttgggccaaa acattccggc aaagtgatgt
     6841 catggaacgg aatggctatt tacggtgccc tcgctgttgg tgcgccgctt ggcctgttga
     6901 ttcatagcca ttacggtttt gccgcactgg cgctcaccac aatggcatta cccttattgg
     6961 cgtgggcctg taacggcaca gtgcgcaaag taccggccct ggcgggagaa cgtccatcgc
     7021 tgtggagcgt tgtcgggctt atctggaaac cagggttagg tctggcacta caaggcgttg
     7081 gttttgcggt tatcgggact ttcgtttcgc tctactttgc cagcaaagga tgggcgatgg
     7141 cgggctttac tnnnnnnnnn ntcatgtttg gctggatgcc ggaccgtttt ggcggcgtga
     7201 aagtggcgat cgtctctctg cttgtagaaa cggtgggctt gttgctgctc tggcaagccc
     7261 caggggcgtg ggtcgcatta gcgggcgcgg cgttaactgg agccggatgt tcgcttatct
     7321 ttcctgcgct gggcgtggag gtggttaaac gtgtcccctc acaagttcgc ggcaccgcac
     7381 tgggcggtta cgccgcgttt caggatatcg ccctcggcgt ctccgggccg cttgcgggaa
     7441 tgctggcgac cacgtttggt tactcttcgg tatttcttgc cggggcgatc tctgcggtgc
     7501 tgggaattat tgtcacgata ctgtcgtttc gtcgcggtta acgaaccagc cagaccagca
     7561 tcaggataat cgcgactaac agtatccaca gcgcgggacg cgttgccata ttttgcaacg
     7621 tgtccatcat cggtggaaaa ggattaagca gcggttgcat atcacgcggc tcaatccctt
     7681 taacccgccg ttgataaagc tggttaagaa tatcctcggc ctgggcagaa gaaagcgatg
     7741 attgcgcaga aaggccatat ttttgctgga tatatgccga taacgccgcc agttcactgg
     7801 catctaaagg ttgttttagc gccatctgta gtgattccag tgtcggcgta ttttgctggc
     7861 ttagcgtctg acgcgcctgt agccaggtca ccagatggtt aaacagtttc gctggaatta
     7921 actcgccatc tttcaccccg gaaagttcca gcatcgattg ccagatctgt ttgctgggtt
     7981 cccccgttgc tgccgcaagt ttggtcacca gctgtttgag cgcattgtgc tccgccggta
     8041 ataaaggacg gtcggtcgcc tcgcgctgct gtggttgcgg aataaccatc ttcccttcct
     8101 gcaacagggt gagaatggtt tttaattgct ccggtgagag ctgattcagc ggcgtctgac
     8161 cnnnnnnnnn natataatcc gtgaccgcct gacgattatt ccccagacgt aaatactccc
     8221 ctaattgcgc taaaagctgg cgggcagaat ggcttttttg cgcggccaac agacgttgcg
     8281 ccagattatg ctcagcggca gggaagtgac gcgaaagcag cggtgaatct cccgacagac
     8341 caatatcgtg cctgataccc gcccacagtt ctgctctttg ttgttgcgtc agtgaggtca
     8401 ctttcgtcat taagctttcc agcgaagtac gttgctgact ggataaaggc tgattgcccg
     8461 cgccagacgg caggttatct ccctgacctg gtggttgccc aggaggaggg ccggaaatag
     8521 gttgtatcat tacgtatcct tatacctgaa atcttcgcaa gtatgcctgg ccgcgagatt
     8581 atggcacact tgtctggtta actctcgtct catacaggta acacaaacgt gaaaatcctt
     8641 gttgatgaaa atatgcctta tgcccgcgac ttatttagcc gtttgggtga ggtgaccgcg
     8701 gttcccgggc gtccaattcc cgtcgctcaa ctggcagacg cggatgcgct tatggtgcgt
     8761 tcggtcacga aagtgaatga atctttgctg gcaggaaaac ccattaaatt tgttggcact
     8821 gccaccgcgg ggaccgacca tgtcgatgaa gcctggttaa agcaggcggg aattggtttt
     8881 tccgctgcac ctggctgtaa tgcgattgcg gtagtggaat atgttttctc ctccctgctg
     8941 atgcttgccg aacgcgatgg attttcactg cacgagcgta ccgtggggat cgttggcgtt
     9001 ggtaacgttg gtcgtcgttt acaggcgcga ctggaagcgt tagggatcaa aaccttactt
     9061 tgcgatccgc cccgcgccga ccgtggggat gagggtgatt tccgctcgct ggatgagtta
     9121 gtccagcatg cggatattct gactttccat acgccactct ttaaagatgg tccgtacaaa
     9181 acgctgcatc tggcggatga aaaactgatc cgcagcctga aaccgggagc gatcctgatt
     9241 aacgcctgcc gtggcgcagt cgtcgataat accgccctgc tgacctgcct gagcgaaggc
     9301 cagaagttaa gcgtagtgct ggatgtctgg gaaggcgaac cggaacttaa cgtggaattg
     9361 ctgaaaaaag tggatatcgg cacgccgcat atcgcaggct ataccctgga aggtaaagca
     9421 cgcggtacta cgcaagtgtt tgaagcttat agcaagttta ttgggcatga acagcacgtt
     9481 gcgctggata cattactgcc cgcgccagag tttggtcgca ttacgctgca tggcccgctc
     9541 gatcaaccga cgctgaaaag gctggtgcat ttggtgtatg atgtgcgccg cgatgacgca
     9601 ccgctgcgta aagtcgccgg gataccgggt gagttcgata agctgcgcaa aaactatctg
     9661 gaacattgcc gtcctgggcg caactggcgc tgtgggcgaa gccctgcttg aaacgctggc
     9721 tgaacgtcag ttcccggttg gggaaattta tgcactggca cgtaacgaaa gcgcaggaga
     9781 acaactgcgc tttggtggta agacaatcac cgtgcaggat gccgctgaat tcgactggac
     9841 gcaggcgcag ctggcgtttt ttgtcgcagg caaagaggct accgctgcct gggttgaaga
     9901 agcgaccaac tcaggttgtc tggtgatcga cagcagcgga ttgtttgctc tcgaacccga
     9961 cgtaccgctg gtggtgccgg aagtaaaccc gtttgtactg accgattacc gcaaccggaa
    10021 tgtcatcgcc gtaccagaca gtctgaccag ccagctgctg gcggcactga aaccgctaat
    10081 tgatcagggc ggtttatcac gtatcagcgt taccagcctg atttcagcct ccgcccaggg
    10141 caaaaaagcg gtcgatgcgt tagcggggca gagtgctaaa ttactcaatg gcattccgat
    10201 tgacgaagaa gatttcttcg ggcgtcagct ggcgttcaac atgctgccgt tactgccgga
    10261 tagcgaaggt agcgtgcgtg aagaacgtcg tatcgttgac gaagtacgca aaatcctgca
    10321 ggacgaaggg ctgatgattt cggcaagcgt cgtccaggca ccggtattct acggtcatgc
    10381 ccagatggtc aactttgaag cactgcgtcc gctggcggca gaagaagcgc gtgatgcgtt
    10441 tgctcagggc gaagatattg tgctctctga agagaacgga ttcccgactc aggtaggaga
    10501 tgcttctggt agtccgcatc tttctgttgg ctgcgtacgt aatgactacg gtatgccgga
    10561 gcaagtccag ttctggtcgg tggccgataa cgttcgcttt ggcggcgcgc tgatggcagt
    10621 aaaaatcgcc gagaaactgg tgcaggagta tctgtactaa tgtccgacca gcaacaaccg
    10681 ccagtttata aaattgcgct gggcattgag tacgacggca gtaggtatta cggctggcaa
    10741 cggcagaatg aagtccgcag tgtgcaggag aagctggaaa aggcgctctc ccaggtggcg
    10801 aacgaaccca tcaccgtctt ctgcgccggg cgtactgacg ccggtgtaca cggtaccggg
    10861 caggttgtgc atttcgaaac gaccgcgcag cgcaaagacg ctgcgtggac gttaggcgta
    10921 aatgcgaatt tacctggtga catcgccgtg cgttgggtta aagctgtacc tgatgatttt
    10981 catgcccgat ttagcgccac ggctcgccgt tatcgctaca tcatctacaa tcatcggctg
    11041 cgcccggcgg tactgagtaa aggagtaacc catttttacg agccgctgga cgctgaacgg
    11101 atgcatcggg ctgcgcaatg cttgctgggc gagaatgatt tcacctcgtt ccgtgcggtg
    11161 cagtgtcagt cccggacccc gtggcgcaac gttatgcata ttaatgtcac gcgtcacggt
    11221 ccttatgtgg tggtagatat caaagcgaat gcctttgtac atcatatggt caggaatatt
    11281 gtcggcagcc tgatggaagt cggtgcccac aaccagccgg agagctggat agcagagttg
    11341 ctggcggcaa aggacagaac gctggcggca gcaacggcaa aagcggaagg gctatatctg
    11401 gtcgcggtgg attaccctga ccgatatgat cttccaaaac cgccaatggg cccgctattt
    11461 ctggcggact aacgagaata atgctcggaa catttcaggc tgtagccaac gtagttacag
    11521 cctgatagat gacgagtaca aggcataggc aaatatggac ctgatttatt tcctcatcga
    11581 ttttatcctg cacattgacg tgcatctggc agagctggtc gcggagtacg gcgtctgggt
    11641 ttatgccatt ttgttcttaa ttttgttctg tgaaaccggt ctggtggtaa cgccgttttt
    11701 accgggtgat tcattgctgt ttgttgccgg agcgttggca tcactggaga ccaacgatct
    11761 caacgtccat atgatggtgg tgctgatgct gatcgccgcg attgtgggtg acgcggtcaa
    11821 ttacactatc gggcggttgt tcggtgaaaa gttattcagt aatcccaatt cgaaaacttt
    11881 ccgtcgcagt tatctcgaca aaacccatca gttttatgag aaacatggcg gcaaaacgat
    11941 tattctcgcc cgttttgtgc cgatcgtcag aacgttcgcc ccgtttgttg cgggaatggg
    12001 ccacatgtcg taccgtcatt tcgccgctta taacgtgatt ggcgcactgt tgtgggtact
    12061 gctttttacc tacgcaggct atttcttcgg cacgctcccc ttcattcaga gcaaccttaa
    12121 actgatgatt gttggcatta tttttgtttc aatattgcca ggtgttttcg aatttattcg
    12181 ccacaaacgc gcagcggcac gcgccgcaaa ataaaaagta actctgcggt tcgaccactt
    12241 ttttatccaa agtttcgggc tgttatgttt taatgtgcaa cattcatggt ctgttggggg
    12301 caaaaatggc attatgcgtc cccaaagata aaactggcat cgaaccaggt tcagacagaa
    12361 aggtccctaa tgagctggat tgaacgaatt aaaagcaaca ttactcccac ccgcaaggcg
    12421 agcattcctg aaggggtgtg gactaagtgt gatagctgcg gtcaggtttt ataccgcgct
    12481 gagctggaac gtaatcttga ggtctgtccg aagtgtgacc atcacatgcg tatgacagcg
    12541 cgtaatcgcc tgcatagcct gttagatgaa ggaagccttg tggagctggg tagcgagctt
    12601 gagccgaaag atgtgctgaa gtttcgtgac tccaagaagt ataaagaccg tctggcatct
    12661 gcgcagaaag aaaccggcga aaaagatgcg ctggtggtga tgaaaggcac tctgtatgga
    12721 atgccggttg tcgctgtggc attcgagttc gcctttatgg gcggttccat gggatctgtg
    12781 gtaggtgcgc gtttcgtgcg tgccgttgag caggcgctgg aagataactg cccactgatc
    12841 tgcttctccg cctctggtgg cgcacgtatg caggaagcac tgatgtcgct gatgcagatg
    12901 gcgaaaacct ctgcggcact ggcaaaaatg caggagcgcg gcttgccgta catctccgtg
    12961 ctgaccgacc cgaccatggg cggtgtttct gcaagtttcg ccatgctggg cgatctcaac
    13021 atcgctgaac cgaaagcgtt aattggcttt gccggtccgc gagttatcga acagaccgtt
    13081 cgcgaaaaac tgccgccagg attccagcgc agtgaattcc tgatcgagaa gggcgcgatc
    13141 gacatgatcg tccgtcgtcc ggaaatgcgc ctgaaactgg cgagcattct ggcgaagttg
    13201 atgaatctgc cagcgccgaa tcctgaagcg ccgcgtgaag gcgtagtggt gccaccggta
    13261 ccggatcagg aacctgaggc ctgataactg ataagggcag ggccactggc tctgcccttt
    13321 tgctattctc accgtaacga atcagcggat accatgatta tcaaacgcac tcctcaagcc
    13381 gcgtcgcctc tggcttcgtg gctttcttat ctggaaaacc tgcacagtaa aactatcgat
    13441 ctcggccttg agcgcgtgag ccaggtcgcg gcgcgtcttg gcgttctgaa accagcgcca
    13501 tttgtgttta ccgttgcggg tacgaatggc aaaggcacca cctgccgtac gctggagtcg
    13561 attctgatgg cggcagggta caaagtgggc gtctacagtt cgccgcatct ggtgcgttat
    13621 accgagcgcg tacgtgcgca gggtcaggaa ttgccggaat cggcccacac cgcttctttt
    13681 gcggagattg aatcggcacg cggtgatatt tccctgacct atttcgagta cggtacgctc
    13741 tcggcgttgt ggctgttcaa gcaggcacaa cttgacgtag tgattctgga agtagggctg
    13801 ggcggtcgtc tggacgcaac caatattgtc gatgccgatg tcgcggtagt aaccagtatt
    13861 gcgctggatc ataccgactg gctggggcca gatcgtgaaa gtattggtcg cgagaaagca
    13921 ggtatctttc gcagcgaaaa accggcaatt gtcggtgagc cggagatgcc ttctaccatt
    13981 gctgatgtgg cgcaggaaaa aggtgcacnn nnnnnnnntc ggtgggagtt cagtgaaaat
    14041 gctattcgcg acgggattgc cagcgcaatt ttgccgggac gtttccagat tgtgagcgag
    14101 tcgccacgcg ttatttttga tgtcgcgcat aatccacatg cggcggaata tctcaccggg
    14161 cgtatgaaag cgctaccgaa aaacgggcgc gtgctggcgg ttatcggtat gctacatgat
    14221 aaagatattg ccggaactct ggcctggttg aaaagcgtgg ttgatgactg gtattgtgcg
    14281 ccactggaag ggccgcgcgg tgccacggca gaacaactgc ttgagcattt gggtaacggc
    14341 aaatcatttg atagcgttgc gcaggcatgg gatgccgcaa tggcggacgc taaagcggaa
    14401 gacaccgtgc tggtgtgtgg ttcgttccac acggtcgcac atgtcatgga agtgattgac
    14461 gcgaggagaa gcggtggcaa gtaagtttca gaatcggtta gtgggcacga tcgtgctggt
    14521 ggcgctgggg gtgattgtac ttccagggct gctggacggg cagaaaaaac attatcagga
    14581 tgagttcgcg gctatcccgc tggtgccaaa agcgggcgat cgcgatgagc ctgatatgat
    14641 gccagccgcc acccaggcgt tgccgacgca gccgccggag ggcgcagcgg aagaggtacg
    14701 ggcaggtgat
//
LOCUS       DAWDRC010000025        14670 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2171_length_14670_cov_2.798426, whole genome shotgun sequence.
ACCESSION   DAWDRC010000025 DAWDRC010000000
VERSION     DAWDRC010000025.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14670)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14670)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2171_length_14670_cov_2.798426"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    850..859
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1365..1374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2119..2128
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4923..4932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3718 a         3520 c         3600 g         3792 t
ORIGIN      
        1 aacaataacg ctacatttgc tggggtgcaa atcggtctgt tgaagagtga gcggggcttt
       61 atcctgcaat gctgattagg gctgacattt tatcctggtt ggcggcttcc cgaggtactc
      121 acgaacggga agccggaaaa gtcactgttg ccccgcccag atgaccagca ctttatcgtc
      181 gccatgctca cgaacaaagc cgtagccctg cttcagcgaa agtgtcgttt gtttgcccgc
      241 gccaattgcg ggatggcggg cgcggaactg gctgattttt tgccagtgcg cgacgctggc
      301 ggcagattta ccgctaacat cctgccagtt catatccgaa cgcgtacctt gcagcggatc
      361 agaaccggta ggaccgaacg gacgcgagga ttcatcgcca taaaaaattt gtaccgcgcc
      421 tggcgctaat aataataact ctgctgcttt gtcgccccct tcacggaaca ggcgggtatc
      481 atgcgacgag aggtagctca acacgttgaa atcctgcaat ttctccgcca tttgctgcca
      541 ggtcgtatcc atctgcgcca gacaatcgac tgctttcgcc gcctgctcct gataatcgaa
      601 attgatcatc gcatcgaagt cgtggcgata gtaatcactt tgcatcacgc cgtggcccca
      661 ggcttcaccg gtcatccaga aaggtttgtc atctaatgct ttgtcggggt tagctttttt
      721 ccattcgcga agtgcagcgc tggcttcggt tttcagttgc tgccaggcgg gcaactcaac
      781 atgtttggcg gtatcgaccc gaaaaccatc aatcccatag tcgcggaccc actgacttaa
      841 ccagtgggtn nnnnnnnnna tcgcgagggg taaagccgtc gatgtcttta gcgtgggtat
      901 ccgttttgtt tttatagaac actggcagac cagaagcgct agttgattcg gttttgatat
      961 ccggcaaaaa ggccagcgac atagtgagat cgtcgaatcc aggattgtcg taatcgccga
     1021 tatccgttct gatccagttt tttccccacc atttatccca gcctgttttg tcgctgaaat
     1081 taatgtaatc gttaaagcta tgctaggttt gcccggcggc aggtttccag tcgctccagc
     1141 gttcacccag cgtttttttc agctcgtcac cagaaagata taacgcgcca aactgatact
     1201 cctgcatatc cgccagcgtg gcatagccgg tgtggttcat cacgacatca aagagaatac
     1261 gaataccgcg ctgatgcgcg ctatcaacca gcgtccgtag atcggcttca ctgcccatat
     1321 tggcatcaag attcgtccag tcctgtgtgt aataaccatg atcgnnnnnn nnnnaaccat
     1381 gataggcata atgcgggaaa tcaccttttg taccgccgcc gacccagccg tgaatttgct
     1441 caaatggggc acttatccat aaagcattga cgcccagttg ctggaggtaa tccagtttgt
     1501 tggtcaggcc gcgtaaatcg ccgccgtgaa aagtgccaat ttccgccata ccgtctttat
     1561 gacgtccgta actctggtca ttactgggat cgccgttttc gaaacgatct gtcagcacaa
     1621 agtaaaccgt ggcgttatgc cagtcgaaag gggcggatgt gtcagtttct gcccgttcca
     1681 gcaggagtaa accgttgctg gtggcagcgg gttgtaacgt tatttgaccg ttcttcacta
     1741 tcgcaatttg ctggctgtaa taatcccgga cagcggctcc ctccgcaaaa gtggcgctga
     1801 catccagtgt gagcggtaat ccatcccatt tcgggcattc acggaccagg cttgctaccg
     1861 gttcgacggt gttctggagg gaaatcatca atgttggcgt accggagcgg gtgtctagtt
     1921 gcagcgtata ttcgccgtcc ctgaacaatc gccattgagg tggcgtgttg ctacaaggtt
     1981 gcagggaaag catctgattg agttttatcg catccgcagg ctgccagcac tgttggtcaa
     2041 aatttagcat tagtggacgc gtacctttgg gcaactgcgc gtggctgaca aatgttcccg
     2101 tcccctgttc gctaaaggnn nnnnnnnnct aaaggcaggg aaccccggag aagtccagct
     2161 ggcggcaacg gcgaagccag gaaggagtgt cagaaaacag gcggcgagtt tcatggcgga
     2221 tgagtcctta ttgctgcgat ttcagacagt ttgccagcga taagccagac aaaactcatc
     2281 cctgagcggt aagtccacag gatgagaagc aagggtgagc gatcgcgcgc aaaaaacggc
     2341 tgaattttgc gataaacccc acattttctg cgatttagcg ccaatctgaa tcgttaactc
     2401 gtgacatagt ttcagatttg gactattcct tggggtgctc ttgacagcta tttttgatat
     2461 gatttgagat tcaactctca aatttgtaaa aaatataagg tgttggaatg attaaatccg
     2521 accaggagac ctgatgattt tgactcccat acgacgatat ggggcgatga ttcttatgtt
     2581 actcactctg gtgttttcga gtgaggtgtt agcgaagacg cacacaacaa cagcgagtca
     2641 aaagtcccac ttaactaaag ctagtaataa acaggtaagc agtaaacaag agtattctcg
     2701 caatagtgca aagagtagtt cacttcctga tttgcgaaaa tacccttccg gaacaccaag
     2761 gaaaaaggcg tttctccgga ctgtaatgcc ttacattacc agccaaaatg cggccattac
     2821 tgcggaacgt aactggctga tttcaaaaca gtatcagggc caatggtcac ctgctgagcg
     2881 tgcgcgtctg aaagacatcg ccaaacgcta caaggtgaag tggtctggta atacgcgaaa
     2941 aatcccgtgg aataccttgc ttgaacgcgt agacattatc cctaccagta tggtggcgac
     3001 gatggctgca gcagaaagcg gttggggaac gtcaaagctg gcgcgcaaca acaacaacct
     3061 gttcggcatg aaatgcatga aaggacgttg taccaatgcg ccgggtaaag tgaaagggta
     3121 ctcacagttt agttctgtca aagaatcggt gagcgcctat gtcactaacc tcaatacgca
     3181 cccggcttac tcttcgttcc gtaaatcgcg tgcacaactg cgtaaagcgg atcaggaagt
     3241 gactgccaca gcgatgattc acaagctgaa gggctactcg accaaaggga agagttataa
     3301 caactacctg ttcgcaatgt accaggataa ccaacggtta atcgcggcgc atatgtaatt
     3361 gcatttcctc gagccttatc cgacttgtca ggcggataag gcttttgctc gtatcaggtg
     3421 gttgtgctga gttacctgat gcgacgccga taattctcat catcgctaca acatgacctc
     3481 gctatttaca tcgcgatact cttttggcgt tgtgtcatat gcttttttaa aaacagagta
     3541 gaaatattgc agcgatggat aaccgcacat ttgcgatatc tcgttaattg acaaggtagt
     3601 agaaatcagc aggctgcgcg ctttctccag cttttcggca tgaatcatgg cgtgaatggt
     3661 ttcgcccacc tcttctttaa aacgcttctc aagattggag cgtgatatgc ccaccgcatc
     3721 cagtacctga tccactttaa tccctttaca ggcgtgatta cgaatgtaat gcatggcctg
     3781 aataacggcg ggatcggtca gcgagcggta atctgttgag cgccgttcaa tgacgcgaac
     3841 tggtgggacc aaaatccgct gtagcggcat ttcttcttta tctaataatc gatgcaacag
     3901 ttttgccgcc tgatagccca tttgccgtgc gccctgagcg accgaagaaa gggcgacacg
     3961 cgacagatag cgggtcagtt cttcgttatc gatgccaatc acgcataatt tttccggtac
     4021 gggaatgtgt agatgttcac atacttgcag aatatgccgc gcccgggcgt cagtaacggc
     4081 aataatcccg gtttgcggtg gcagcgtttg tagccagtct gccagccgat tttgcgcgtg
     4141 ttgccagttc tctggcgcgg tttctaaccc ctgataaacc actccgcgat acttttcttc
     4201 ggcgacaagc tgacgaaatg catattcgcg ttcagtggcc caacgtttac cgcttgattc
     4261 cggaagacca taaaaagcaa agcggttaac gcctttctct tttaaatgca aaaatgcgct
     4321 ttcaaccagc gcatagttat cggtggcaat gtaatgaacg ggtgggtaac tttcggcaag
     4381 gtgatacgag ccaccaaccc caacaatggg gacgtcgaca tcagccagcg cttgctcgat
     4441 ttgtttgtcg tcgaagtcgg caatgacgcc atctcctaac cagtccttga ttttatcaat
     4501 gcgggcgcgg aaatcttctt caatgaaaat atcccattcc gattgtgacg cctgtaaata
     4561 ttcccctacg ccttctacta cctgccggtc ataggcttta ttggcattga acagtaatgt
     4621 gatgcggtga cgtttagtaa acatggttct tttcctgctg aatcatgcaa aaactcaaaa
     4681 ccggtaatac gttaccggct ttgagaaaat ttttatcaaa atcaagaacg gcgtttggtt
     4741 gcggagtcca tccatacagc cagcaacaga atcgcacctt taacgatata ctgccagaag
     4801 gtcggtacat ccatcatact catgccgtta tccagtgaag ccatgatgaa tgcccccatt
     4861 accgctccag ccacgctccc gacgccgcct gccagactgg tgccgccaat cacacatgct
     4921 gcnnnnnnnn nngaaccagc gccgagtcga gaactgagga ttaatccggc gatggctacc
     4981 attaatccgt taatagcgaa cacggcaagt ttggtgcgtt caacgttaat cccggagaga
     5041 cgtgccgctt ccagattgcc gccgatggca taaatgcgtc gtccaaatgc cgtccgcgtt
     5101 gccataaaca ttccgccgag taacagcaac gtcagcagca gaacaggagt gggaacacca
     5161 cggtaatcat tcaacagcca gattgcgcct aatacgatga tagcggttaa agcctggcga
     5221 ccgactactg cggtagaggc cggagactgc aaacccaaag cctgacggcg cattcttccg
     5281 cgccattgcc agccaacaaa agccattaag ccaagcgcgc caatgatgaa gccggtgctg
     5341 gcaggtagat agctttgccc aatttgtgac atcgcggcgc tggtggggga aacagtcgtg
     5401 ccgttggtga tgccaatgag tatgccacga aatgccaaca tgcccgcgag ggtgacaata
     5461 aatgaaggga ctttgcggta cgcgacccac catccgttcc aggctccgag aagcagtccc
     5521 agaaccaacg tcacaatgat ggtaagtggc aaaggccagc ctagccagac gtcacaaatc
     5581 gccgcgacgc cacctaacag ccccatcatt gagccgacgg aaaggtcgat ttcagcagaa
     5641 attatgacga acaccattcc taccgcgagg atgccggtaa tcgcggtttg gcgtaacagg
     5701 ttggagacgt tacgggcgct taagtaggca ccatcggtgg tccaggtaaa gaacagcatg
     5761 attgcgatga tagctgcaat catcacgaag acctgcaaat tcagtgattt cagcccggaa
     5821 aggccaccgg atgtcggtac ggctaatttc acttcagacg gattgctttt cgacatgatg
     5881 ttcgctcctc aatgcggctt ccatcacctg ctcctgagtc aggttatgat ttatcaggtt
     5941 ggcttttagt ttcccttcat gcatcaccag tacacgatcg ctaaggccga gcacttcagg
     6001 taattcggaa gagatgacaa taacggcaat accctgctgg acgagttggt taattaattt
     6061 gtagatctcg tatttcgcgc caatatcgat acccctggtg ggttcatcaa gaatgagaat
     6121 gcgcgggtta agtaacagac agcgagcgag gatcgctttt tgttgattgc cgccgctcaa
     6181 acgtccaata gcaaggtcgg gggacgacgt tttaactttg agttgctgga ttgattccag
     6241 aatacatttt tgctctgccg cgtcatcaag ctggctaatg ccaccggtaa attttttgag
     6301 tgcggcgagg gtaatatttt taccaaccgc cattaccgga acgatgccgt cgcgctttct
     6361 gtcttcgggt accatcgcaa tcccctgggc gatggcttgc tgacagttac gaatatctac
     6421 ctgtttgccg tcaatataaa ttttcccttc ccattgtccg ggccacacac caaacaggca
     6481 ctgaatagtc tcggtacgtc cggcaccaac gagtccggcg atacccagta tttcgccacg
     6541 tttcagggaa aacgagacat cattaactcg tttaatatga cgattaaccg gatgccatgc
     6601 cgtcagatgt tcaatacgta atatttcatc tccggtggta tgtggttcat tagggtaaag
     6661 cgcggttaac tctcgcccga ccatcatggt gataatatcg tcttcactca ttccagcagc
     6721 atcacgcgtg ccaatgtgct gaccgtcgcg aataacgcaa atcgtatcgg aaatcgcttt
     6781 gacttcgttg agtttgtgcg aaatataaat acaggcgata ccgtgctgtt gtagatcgcg
     6841 aataatatcc agtaaaaccg acgtttcctg ctcagttaat gaggctgtcg gctcatcgag
     6901 aattaacaaa cgcacctgtt tattaagtgc cttggcaatt tcaaccagtt gttgttgccc
     6961 aagtcctaaa tcgccaacgc gggtatcagg tgaaatggat aaactgacct gtgcgagcag
     7021 cttctgacag cgtagcgtca tcaggtcata atccataatg ccattatggg ttatttcgtt
     7081 acccaggaag atattttcca gcacggtcag ttctttcacc agcgccaatt cctggtgaat
     7141 aatggcgata cctttgcgtt cggtatcgcg gatgtgactc gcctgaatct cttctcccgc
     7201 aaaaataatt tcgccttcgt aggaaccatg gggataaata ccacacagca ctttcatcag
     7261 cgttgattta cctgacccat tttccccaca gagtgagacg atttcgccag cattcaaccg
     7321 caagctgacg ttatcaatcg ccttcacact gccgaaggtt ttggtaatgt tcttcatttc
     7381 aagtagataa ggcataacga ctccacctaa gccaatcaat tcacgcggca tggagagaaa
     7441 tcacgccccc gctccgcgcc ggggcgtaac gcttacagct cgctctcttt gtggaatccg
     7501 tctttaacta ccgtatcttt gatgttgttt ttattcacat cgatcggtgt cagcaggcgg
     7561 gagggaacat ctttcaggcc attattcagt gtggtatccg cttttggctc ctgaccattg
     7621 cccaactcaa cggcaatttc tgcggcagta tttgccaaca atgtaatagg tttatacacc
     7681 gtcatagttt gcgtaccggc agcaatacgt ttaatacctg caagatccgc atcctggccg
     7741 gaaattgcta ctttccctga taaaccttgc gcgcttaatg cctgaattgc ccctcctgcg
     7801 gtggcatcgt ttgaggcaac tacagcatca attttgttat tattggcggt tagcgcgttt
     7861 tccataattt tcaatgcgtt ttccggtaac cagccatcaa cccattggtc accaacgact
     7921 ttaatttttc cggaatcaac ataaggtttt aatactttca tttgcccggc gcggaacagc
     7981 ttggcgttgt tatctaccgg cgagccgccc atcaggaagt aattaccttg cggaacaata
     8041 tcgaccaggg cttttgcctg cagttcaccg actttttcat tatcgaaaga aatataaaaa
     8101 tcgatatccg catcgttaat catacggtcg taagctaata ctttaatacc ttcttgtttg
     8161 gcttctttta caacgttact taatacctga ccgttataag gaataatgac aagaacatcg
     8221 acaccccggt taatcatgtt ttcaatctgc gatatttgtg tttcttcatt gccatttgca
     8281 gactgtacaa atactttcgc gccgagagat tctgcttttt tcacaaaaat atctcgatct
     8341 ttttgccagc gttcaagacg gagatcatca atcgccatac ctattttgac ttctttggcg
     8401 tgtgccgcaa cgttggtaag cagaagtgag gtgcaaaggg tgagtagaat gttctttatt
     8461 ttcatggtgt agggccttct gtagttagag gacagcttta ataagtaaca atcaccgcga
     8521 taaacgtaac caatttttag caactaaaca ggggaaaaca attacagatt tttatctttc
     8581 gattacgatt tttggtttat ttcttgattt atgaccgaga tcttactttt gttgcgcaat
     8641 tgtacttatt gcatttttct cttcgaggaa ttacccagtt tcatcatccc attttatttt
     8701 gcgagcgagc gcacacttgt gaattatctc aatagcagtg tgaaataaca taattgagca
     8761 actgaaaggg agtgcccaat attacgacat catccatcac ccgcggcatt acctgattat
     8821 ggagttcaat atgcaagcct attttgacca gctcgatcgc gttcgttatg aaggctcaaa
     8881 atcctcaaac ccgttagcat tccgtcacta caatcccgac gaactggtgt tgggcaagcg
     8941 tatggaagag cacttgcgtt ttgccgcctg ctactggcac accttctgct ggaacggggc
     9001 ggatatgttt ggtgtgggag cgtttaatcg tccgtggcag cagcctggtg aggcactggc
     9061 gttggcgaag cgtaaagcag atgtcgcatt tgagtttttc cacaagttac atgtgccatt
     9121 ttattgcttc cacgatgtgg atgtttcccc tgagggcgcg tcgttaaaag agtacatcaa
     9181 taattttgcg caaatggttg atgtcctggc aggcaagcaa gaagagagcg gcgtgaagct
     9241 gctgtgggga actgctaact gctttacaaa ccctcgctat ggcgcgggtg cggcgacgaa
     9301 cccagatcct gaagtcttca gttgggcggc aacgcaagtt gttacagcga tggaagcaac
     9361 ccataaattg ggcggtgaaa actatgtcct gtggggtggt cgtgaaggtt acgaaacgct
     9421 gttaaatacc gacttgcgcc aggagcgtga acagctgggc cgctttatgc agatggtggt
     9481 tgagcataaa cataaaatcg gtttccaggg cacgttgctt atcgaaccga aaccgcaaga
     9541 accgaccaaa catcaatatg attacgatgc tgcgacggtc tatggcttcc tgaaacagtt
     9601 tggtctggaa aaagagatta aactgaatat tgaagctaac cacgcgacgc tggcaggtca
     9661 ctctttccat catgaaatag ccaccgccat tgcgcttggc ctgttcggtt ctgtcgacgc
     9721 caaccgtggc gatgcgcaac tgggctggga caccgaccag ttcccgaaca gtgtggaaga
     9781 gaatgcgctg gtgatgtatg aaattctcaa agcaggcggt ttcaccaccg gtggtctgaa
     9841 cttcgatgcc aaagtacgtc gtcaaagtac tgataaatat gatctgtttt acggtcatat
     9901 cggcgcgatg gatacgatgg cgttggcgct gaaaattgca gcgcgcatga ttgaagatgg
     9961 cgagctggat aaacgcatcg cgcagcgtta ttccggctgg aatagtgaat taggccagca
    10021 aatcctgaaa ggccaaatgt cactggcaga tttagccaaa tatgctcagg aacataattt
    10081 gtctccggtg catcagagtg gtcgccagga gcaactggaa aatctggtaa atcattatct
    10141 gttcgacaaa taacggctaa ctgtgcagtc cgttggcccg gttatcagta gcgataccgg
    10201 gcattttttt aaggaacgat cgatatgtat atcgggatag atcttggcac ctcgggcgta
    10261 aaagttattt tgctcaacga gcagggtgag gtggttgctg cgcaaacgga aaagctgacc
    10321 gtttcgcgcc cgcatccact ctggtcggaa caagacccgg aacagtggtg gcaggcaact
    10381 gatcgcgcaa tgaaagctct gggcgttcag cattctctgc aagacgttaa agcattaggt
    10441 attgccggac agatgcacgg tgcaacctta ctggatgctc agcaacgggt gttacgccct
    10501 gccattttgt ggaacgacgg gcgctgtgcg caagagtgca ctttgctgga agcgcgagtt
    10561 ccgcaatcgc gggtgattac cggcaacctg atgatgcccg gatttactgc gcctaaattg
    10621 ctatgggttc agcggcatga gccggagata ttccgtcaaa tcgataaagt attattaccg
    10681 aaagattact tgcgtctgcg tatgacgggg gagtttgcca gcgatatgtc tgacgcagct
    10741 ggcactatgt ggctggatgt cgcaaagcgt gactggagcg atctcatgct gcaggcttgc
    10801 cacttatctc gtgaccagat gcccgcatta tacgaaggca gcgaaattac tggtgctttg
    10861 ttacctgaag ttgcgaaagc gtggggtatg gcggcggtgc cagttatcgc aggcggtggc
    10921 gacaatgcag ctggtgcggt tggtgtggga atggttgatg ctaatcaggc aatgttatcg
    10981 ctggggacgt cgggggtcta ttttgctgtc agcgaagggt tcttaagcaa gccagaaagc
    11041 gccgtacata gcttttgcca tgcgttaccg caacgttggc atttaatgtc tgtaatgctg
    11101 agtgcggctt cgtgtctgga ttgggccgcg aaattaaccg gcctgagcaa tgtccctgct
    11161 ttaatcactg cagctcgaca ggctgatgaa agtgccgagc cagtttggtt tctgccttat
    11221 ctttccggcg agcgtacacc acacaataat ccccaggcga aaggggtttt ctttggtttg
    11281 actcatcaac atggccccaa tgaactggcg cgagcagtgc tggaaggcgt gggttatgcg
    11341 ctggcagatg gcatggatgt cgtgcatgcc tgcggcatta aaccgcaaag tgttacgttg
    11401 attggtggcg gggcgcgtag tgagtactgg cgtcaaatgc tggcggacat cagcggtcag
    11461 caactggatt accgcacggg tggcgatgtg gggccagcac tgggcgcagc aaggctggcg
    11521 cagattgcgg cgaatccaga gaaatcgctc attgagttgt tgccgcaact accgttagaa
    11581 cagtcgcatc taccagatgc gcagtgttat gccgcttatc agccacgacg agaaacgttc
    11641 cgtcgcctct atcagcaact tctgccatta atggcgtaaa cgttatcccc tgcctgaccg
    11701 ggtgggggat aattcacatc tatatatata aataattaat taatatttag tatgaattta
    11761 ttctgaaaat catttgttaa tggcattttt cagttttgtc tttcgttggt tactcgtaat
    11821 gtatcgctgg tagatatgga gatcgttatg aaaacctcaa agactgtggc aaaactatta
    11881 tttgttgtcg gggcgctggt ttatctggtt gggctatgga tctcatgccc attgttaagt
    11941 ggaaaaggtt attttcttgg cgtgttaatg acggcaactt ttggcaacta tgcatatctt
    12001 cgcgcagaaa aacttgggca actggatgat ttttttatcc acgtctgtca gttggtagcg
    12061 ttaatcacta tcggtctctt gtttatcggt gttttaaacg cacctatcaa tgcttatgaa
    12121 atggtgatct accccatcgc cttttttgtc tgcttgtttg gtcaaatgcg tttgtttcgc
    12181 tcggcatgag caacataaag ttcttacaca ttcaggaatg aaaggaatac tgtgatggac
    12241 aacaaaatat caacctattc tccggcattt agtattgttt catgggtagc ccttattggc
    12301 ggtattgtta cctatctgtt aggactatgg aatgcagaga tgcagttaaa tgaaaaaggg
    12361 tattattttg ccgtactggt attaggtttg ttttccgcgg cgtcttatca aaagaccgtt
    12421 cgggacaagt atgaaggcat acctaccacc cccatttatt atatgacttg cctgacggtc
    12481 tttattatct ctgttgcgtt gttgctggta ggtctatgga atgcaacgtt attactcagc
    12541 gaaaaaggct tttatgggct ggctttcttc ttaagcttgt ttggtgcagt agcggtgcag
    12601 aagaatattc gtgatgccgg aataaaccca ccaaaagaaa cacaggttac ccaggaagaa
    12661 tacagcgaat aattcacata agcccggtca gtccaatgtg accgggcttc tacttaactc
    12721 actaatctgt ttctgtcgat tcgttgtacc agcatagaaa gtaataaact cactgccagg
    12781 gtcgcgcaaa agatccaaat aatatcgagt attggccagt ttttaagctc aatcccccgg
    12841 gtgcgcagtg catggataat caatgcatgg aacccgtata tacctaacga atggcgggag
    12901 attaagccaa gtccgcaaat agtacgcgta tacagcgtgt ttttaaccag agtcaataac
    12961 gcgattgcgc agataaaaac catcggccca cagtaaagat accaggtatc ggcaaaattt
    13021 ccgcgccact gtaattcata taatgtcccg cgagagataa taaaaacccc cgtcgcaaac
    13081 agtgcggcgc tcacccacga cagtgcttta tgctgtgtgt ccatcatccc tatagcgcgg
    13141 cccaacatgc cataaaggat gtagtaaaaa gtatcgccat tgatatataa gttaattggc
    13201 agccattcaa aaccgtcgat tttctgcggc acggtgtttg ggttagcgat gacaccaatc
    13261 accaccatta gcaccagcaa catttttccg ccgacgttct tcacctgaat cagcggtgaa
    13321 accagataaa tcactgcaat cgcgaagaaa aaccataaat ggtaaaacac tggcttttgc
    13381 agcaggtttt tcagcgctaa ctccacattg atggaggtaa acagcgcaat gtagagcagc
    13441 gcgattgcgc tataaaaaaa caggcataag ccgatacgca agaaatggcg cgactgggcg
    13501 ctgcgttcgc caaaaaagag atagccggaa atcatgaaaa atagcggcac gctgacacga
    13561 gaggcagaat tcagaacatt ggcgatatcc caggtgatgg ggctaacact atgagcattg
    13621 gtcacatacc aggtagtggt gtgaatcatc accaccatta aacacgctat ccctcgcagg
    13681 ttatcaatcc agtaaatttt gggctgcatc tgtgtctctg tatctggtta aaaaaagtct
    13741 gaccgataaa tcattcagtt ggcgcactgg aataatctga gttttatcac tacagcttat
    13801 agaggcttaa ggaaattcgt aagatatcag ccactatacc gatataaata ataagactca
    13861 cctgcaaacc agacggtaat ttaatgatga tgaacgcttt ctttccagca atggcgctta
    13921 ttgtgctagt gggttgttct acaccgccac ccgtgcagaa agcacaacgg gtaaaggttg
    13981 atcctctgcg ttcgttgaat atggaagcgt tatgcaagga tcaggcggca aaacgttata
    14041 acaccggcga gcaaaaaatc gacgtcaccg ccttcgaaca gttccaggga agctatgaaa
    14101 tgcgcggtta taccttccgg aaagagcagt ttgtctgttc ttttgacgcg gatggccatt
    14161 ttttgcatct ttccatgcgt taagccctgc tttttcccgt ttcgtactgt atatcttcca
    14221 tccagcgggt atactgatcc cttcctttaa atccacacgt atccagcacg aaataatatg
    14281 caaaagtttg ataccaggac cttccagggc ttgatcctga ccttacagga ttactgggct
    14341 cgccagggct gcaccattgt tcaaccattg gacatggaag tcggcgcggg aacctctcac
    14401 ccaatgacct gtctgcgcgc gctggggcca gaaccgatgg cggctgctta tgttcagcct
    14461 tctcgtcgcc cgaccgatgg tcgctacggc gaaaacccca accgtttaca gcactactat
    14521 cagttccagg tggtcattaa gccatcgccg gacaatattc aggagctgta cctcggttct
    14581 ctgaaagagc tgggcatgga cccgaccatc cacgacatcc gtttcgtgga agataactgg
    14641 gaaaacccga cgctgggtgc ctgggggctg
//
LOCUS       DAWDRC010000026        14232 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2251_length_14232_cov_2.594484, whole genome shotgun sequence.
ACCESSION   DAWDRC010000026 DAWDRC010000000
VERSION     DAWDRC010000026.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14232)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 14232)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2251_length_14232_cov_2.594484"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1697..1706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6629..6638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9302..9311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11189..11198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3530 a         3857 c         3491 g         3314 t
ORIGIN      
        1 tgatggtgat accacgagct ttttcttccg gcgcgttatc gatctggtcg aatgcacgag
       61 cagcaccgcc gtaggtttta gccagtacgg tggtgattgc agcggtcaga gtagttttac
      121 cgtggtcaac gtggccgata gtaccaacgt taacgtgcgg ttttgtacgt tcaaattttt
      181 ctttagacac ggctatattc cttactatag tgctctcccc ttcaggagag agcacgggac
      241 tttggtatta acccttaggc ttatttacca cgggcttcaa ttacggcctg agcaacgtta
      301 ctcggcgctt catcatactt caggaattcc atagtgtatg atgcgcgacc tttggtcaga
      361 gaacgcagct gagttgcata tccgaacatt tcagacagcg gtacttcagc gtggatctta
      421 acgccagtaa cttcagattc ctgacctttg agcataccac gacgacggct caagtcaccg
      481 ataacgtcac cggtgttctc ttcaggagtt tctacttcaa ccttcatgat cggctcaagc
      541 agaactggtt tcgctttctt aaagccttct ttaaaggcga tagaagcagc cagtttaaac
      601 gccagttcag aggagtcaac gtcatggtaa gaaccgaagt gcagacgaat acccatgtct
      661 actaccgggt agcctgccag cggacctgct ttcagctgtt cctggatacc tttatcaacg
      721 gccgggatgt attcgccagg gattacacca cctttaatgt cgttgatgaa ctcgtagcct
      781 ttcgggtttg aacccggctc cagcgggtac atgtcgataa caacatgacc atactgacca
      841 cggccaccag actgtttcgc gtgtttacct tcaacatcgg taactttctg gcggatagtt
      901 tcacggtaag caacctgcgg tttacctacg ttcgcttcaa cgttgaattc acgcttcata
      961 cggtcaacga tgatgtcgag gtgcagttcg cccatacccg cgatgatggt ctggttagat
     1021 tcttcgtcag tccatacacg gaaagacggg tcttctttag ccagacggcc cagagccaga
     1081 cccatttttt cctggtcagc tttggttttc ggttcaactg cgatggagat taccggctca
     1141 gggaattcca tacgttccag aatgatcggc gcatccgggt cacacagggt gtcaccagtg
     1201 gttacgtctt tcagaccgat tgcagctgcg atgtcgcccg cgcgaacttc tttgatctct
     1261 tcacgtttgt tagcgtgcat ctgaacgata cgaccgaaac gctcacgtgc agctttcacg
     1321 gagttcagta cggtatcacc agagttaacc acaccggagt acacacggaa gaaggtcagg
     1381 ttaccaacaa acgggtcggt agcgattttg aacgccagtg cagagaacgg ctcgtcatca
     1441 cttgcgtgac gctcagccgg agtgtcttta ccgtcgtcca ggataccgtt gatcgcaggt
     1501 acgtcaaccg gggatggcag gtaatcaatt accgcatcca gcatcgcctg aacacctttg
     1561 ttcttgaacg cagaaccaca ggttaccagg atgatttcgt tgttcagaac gcgctgacgc
     1621 agagcacctt tgatttctgc ttcagtcagt tcttcaccac ccaggtattt ttccatcagc
     1681 tcttcagaag cttcagnnnn nnnnnntagc tttcattttc accaggtcaa caacaccggt
     1741 gaaatgttct tcagcaccaa tcgccagctg cagcggaacc gggttcgcgc ccagacgggt
     1801 tttgatctgg ttaacaactt tcaggaagtt cgcacccatg cggtccattt tgttaacgaa
     1861 cgcaatacgc ggaactttat atttgtttgc ctgacgccat acggtttcag actgcggctg
     1921 aacaccacca actgcgcagt aaaccattac cgcaccatcg agaacacgca tggaacgttc
     1981 tacttcgatt gtgaagtcaa cgtgccccgg ggtgtcgatg atgttgatgc gatgcggctc
     2041 atactgctta gccataccag accagaatgc agtagtcgca gcggaagtga tggtaatacc
     2101 acgttcctgc tcctgctcca tccagtccat ggttgcagcg ccgtcatgaa cttcaccgat
     2161 tttatggttt acaccggtgt agaacagaat acgttcggta gtagtggttt taccggcgtc
     2221 gatgtgcgca ctgataccga tgttacggta gcgtgcgatg ggtgttgtac gagccatttg
     2281 tttcctcgtt tatcttttag gcgttcaatt taagtagccc aaagcgggct gcttactgga
     2341 agcgcccgcc tggtgactaa aactccgaag ggattaccaa cggtagtgtg cgaacgcctt
     2401 gttggcttcg gccatacggt gaacgtcttc acgtttctta actgcagtac ctttgttttc
     2461 tgcagcatca gaaagttcgt tcgccaggcg cagagccatg gatttatcac cgcgtttacg
     2521 agcagcttca acgatccaac gcattgccag agcattacga cgaaccggac ggacttcaac
     2581 tggtacctga taagtagaac caccaacgcg gcgagactta acttctacag tcgggcgcac
     2641 gttttcgaga gctacttcga atgcttccag ttcagattta ccagagcgct gagccagggt
     2701 ctccagcgcg ctgtatacga tagattcagc agtagatttt ttaccatcta ccatcaggat
     2761 atttacaaat ttagccagca gttctgatcc gaacttcgga tccggcagaa ttttacgctg
     2821 accaatgacg cgacgacgtg gcatggaaat actccgttgt taattcagga ttgtccaaaa
     2881 ctctacgagt ttagtttgac atttaagtta aaacgtttgg ccttacttaa cggagaacca
     2941 ttaagcctta ggacgcttca cgccatactt ggaacgagcc tgcttacggt ctttaacgcc
     3001 ggagcagtca agcgcaccac gtacggtgtg gtaacgaaca cccgggaggt ctttaacacg
     3061 accaccacgg atcaggatca cggagtgctc ctgcaggttg tgaccttcac caccgatgta
     3121 ggaagtcact tcgaaaccgt tagtcagacg aacacggcat actttacgca gcgcggagtt
     3181 cggtttttta ggagtggtag tatatacacg agtacatacg ccacgttttt gcgggcatgc
     3241 ttccagcgca ggcacgttgc ttttcgcaac tttgcgagca cgtggtttgc gtaccagctg
     3301 gttaactgtt gccattaaat agctcctggt tttagctttt gcttcgtaaa cacgtaataa
     3361 aacgtcctca cacaatatga ggacgccgaa ttttagggcg atgccgaaaa ggtgtcaaga
     3421 aatatacaac gatcccgcca tcaccaggcc atctggctgg agtgcttaac cgtaagtctg
     3481 acgaaatcag tatagtcaat gggaatgatg tcgttcgaaa ttcgaccagt caaaccgcgg
     3541 gcaataaggt cttcgttcag agcatagacc ttaatggggg cattacgcag actttcaagg
     3601 tagcggttgc cgtcaactgc ggcagttacg ccatcttgca ataatagcag ttcgtcacct
     3661 tcactgagca gacgcagcag cgcagcaaag tccgtcagcc agggtgaacg atgtaatgtg
     3721 tgcagcataa cgcctcaaaa cctcaaaata acatcgtagt tggcgagttc gcggcgtaag
     3781 gcatctgctt cgagcggcgt ggcttcgacc acaaagggtg tctgcggatc taacccacgt
     3841 tcgcgcagtg aagccgcgca aatccagcac tgttcaatat cgtacagacc caacaattta
     3901 aaagtggcaa tgtaatcacg cgccagcact gcatcgggct tttgtcctgg cagcagctga
     3961 aaaacgccat cagcaataaa gaagacggcc agatcgtcag ttaatgcgga agttgccagt
     4021 aaagcatcta aaccttcccg gcctgcagtt gtaccatgag gcgcagtaga aaaaacaaac
     4081 gcaattcgtt tcatcagaac tgtaccaccc tgtcacaggt cagcgaggct tccgccagcg
     4141 cgccaagtcc gcttaaggta aatcccggct gaaggtttga cgaagcgagc cccagtcttc
     4201 cggcctccgt ttcatcaaca acgccacggc gtaatgctgc cgctacgcag atattcagcg
     4261 ccacaccatg ttgcgcattc aactgttgcc agccgcgcac aaggtcaaat tcgtcgcttg
     4321 ccggtgaggt caattggtta gcgttataga ccccttcccg atagaaaaaa acgctgctta
     4381 actcatggcc ttctgctatc agcgcctgcg caaactgaaa agcactactc gcctgttgcg
     4441 taccgtatgc tggcccggtc accacgatgg caaaacgcat tacttatctt gcccctggaa
     4501 atcaccgctc ttgaactggc ggatgtagag atagacagtg tgtttggaga tgttcaggcg
     4561 gtcagcaacc tggttgatcg cgtctttaat atcgaagatc cctttctcgt agagatttag
     4621 cacgatctga cgatttttgg cgttattaga aacattgcga tcggcattca cttcttcgat
     4681 ggtgaactcc agcgtttggg taaccagatc ttcaacagaa gaggcaaagt tgacgcttga
     4741 accgacatcc ggagtttccg gcggcacaaa agtgctcata atctgcgaga agggaacatc
     4801 aagattcata ttgatgcaca gcagaccaat tacacgctgt tcgcggttac gaatagcgat
     4861 agtcagggac ttcattaata cgccgctttt ggcgcgagta aagtagcatt tagaaacgct
     4921 gctatccgct cccgtcatat cgtgcagcat acgtagcgcc aggtcagtaa ttggcgaacc
     4981 aatcttccgg cctgtatgtt caccgttagc aatgcgaatg gctgaacatt ttagatcctg
     5041 caaagagtgc aaaacgattt cacagtggga gccaataagc atcgctaacc cgtccaccac
     5101 cgcttcgtag gatttcagaa tatcaaaatc ggtctggtca aaaggacgtt gatccagtaa
     5161 atccaactca ctggtttcgt tggttaaaag cgacctggac atgaaaaaaa acactccttt
     5221 tcaggagcct gtcgttaact tttcagggca ggctcattaa tgatgcgggt aactaaatta
     5281 atacagcgga ggttccgctt tccagcacta attatatccg gcctgtaata aaaaaaccgc
     5341 cgcctggtca ggcggcggtt cttaatgctt attttttagc agagtctgcg gctttcgcat
     5401 tagcttccgg ctttgcatca gccttcggcg ctggtttcac atccagcagc tctacgtcaa
     5461 acaccagggt agaattcggt gggatccccg gaacacccgc tttgccgtaa gccagttctg
     5521 gtggaataac cagtttgatc ttaccgcctt tcttgatgtt cttcagacct tctgtccaac
     5581 ccgggataac accgtccaga cggaaagaga gcggttcacc acgggtgtaa gagttgtcga
     5641 actctttacc gtcgatcagc gtacctttgt agttcactac aacagtatcg ctgtctttcg
     5701 gggcttcgcc tttaccagct tctactacct gataaaccag accagtggaa gaggttttca
     5761 cacctttctc tttggcaaat ttctcgcggt actctttacc ttttgcttcg ttatcagccg
     5821 cgtctttttc catcttcgcc tgagcagaag acttcacgcg agcttcgaat gcttgcagag
     5881 tctgttcgat ctcttggtcg gaaagtttgc tcttatcagc aaatgcatcc tgaacaccag
     5941 cgatcagctg atctttatcc agtttgatgc ccagtttttc ttgttcttta agagagtttt
     6001 ccatgtaacg acccagcgaa gcacccagtg cataagctga tttctgatcg tcatttttga
     6061 acgctgcttt gctgtcagca gttgtagcag gttttgcagc ttcagcagca aaagtgattg
     6121 gtgcatgcag ggcaacggcc attgtggtcg ccagcagcgt tactttaaac agtgatttca
     6181 tccatatctc caggatcggg gcatctcacc ccagggttaa ctattatcag aagggtacta
     6241 taaagcgttg tcgaacaaat ctacatacag acacgcccta ttatcatcta ttttcagact
     6301 ctttttgttt aaattagttt cgatgaccgc gaaatgagtg ctgtctcggg cagcaaagtt
     6361 aagtagaatc cgcggcgacc attcgacaaa agaggtgaat catgcaggat ttatcattgg
     6421 aagcacgcct ggcagagctg gagagccgac tggcttttca ggaaatcacc attgaagaac
     6481 tgaacgtcac ggtgaccgct catgaaatgg agatggcgaa actgcgcgat catctgcgtc
     6541 tgctgaccga gaagttaaaa gccagccagc cgtcgaacat cgcgtcgcag gctgaagaaa
     6601 cgcctccgcc acattattga ggcgtaaann nnnnnnnngc gtaaaaaaag cggggattcc
     6661 ccgctttttt gtcacctttt cggtattagt ggcaaccgca accgccgttg cctttaccgc
     6721 cacagcagcc ttcgccaccg tgttcatgac cgtgatcgtg gccatgacca ccgcagcaac
     6781 catcgtggtc gtgatcgtgg tggtgatcgt gcgcgccgtg aacgtgacca tgagccagtt
     6841 cttcttcggt cgcttcgcga atcgccacaa cttcaacgtt gaatttcagg ttctgaccag
     6901 ccagcatgtg gttaccatca accactacgt gatcgtcttc aaccgcagtg atttcaaccg
     6961 gtaccggacc ctggtcggtt tcagccagga aacgcatacc tacctgcagt tcatcaacgc
     7021 ccataaatac gtctttagga acgcgctgca ccaggttttc gtcgtactga ccgtaagcgt
     7081 cgttcgcgcc aacagcgaca tcaaatttgt cgccaacttc atgaccttcc agcgccgttt
     7141 ccaggccaga gatcagggaa ccgtgaccat gcaggtagtc cagcggcgca ctcaccggag
     7201 actcatcaac caacacaccg tcttctgtac gtacctgata ggccaggctg accaccaggt
     7261 cttttgctac tttcatgata tctcctgagc atgggaagaa tagtggcgca gattgtagcg
     7321 gaattctgca gccgtgtact cactagctta aaaaaactcg gcgcatatcg ctagtccgga
     7381 tgaaaaatcc cgatcacttg ctcatctttg cgaacgtgat cgcgggcttc tttgtctgct
     7441 tctcgcatct gatgtccaca cttaacacat tcaacgatat caatattatt ttcgcgccac
     7501 atcgccattg aatcctgtgc ctgacaggcc gggcattttg cgcccgcaat aaaacgtttt
     7561 cggattgcca tctttaccct ctactcaaat tcatcccagc cgtccagctg gcgtcgttct
     7621 tgttgcattt cacgctggaa aatctcttcc agttcgcgtc gggcttccct ggcgcgagaa
     7681 atttgtacgg tatcagcatg catcgggatt aactcccgca gcattcgcat atccaggcgg
     7741 cgaaaatgca gttgcgcgcg ctgcgcctga tgcggatgca tgccaagcgt gaccagcgtc
     7801 ttgcgcccca gctctaacgc actggagaat gtttcacggg aaaactgcgt cacccctgcc
     7861 tgtaataact catgcgcttc cacacgtccg cgcgctcgcg caagaatatg caaatgcgga
     7921 aagtgctgct ggcatatttc caccagcttc atagtgtctt ccggctcgtt acaggtaatg
     7981 acgatagact cagcggcctc tgcacccgca gaacgtaaaa gatctacctg cgtggcgtcg
     8041 ccgtaataaa ctttgtagcc atatttgcgc atcaggttaa cggcactgat atcccgctcc
     8101 agtacagtaa tgcgcatttt atttgccatc agcaaacgac caatcacctg accaaaacgc
     8161 ccaaagccca cgacaatgac ctgcggttta tcatcgttga cccatggttt ttcgtcttct
     8221 tcttccggtc cgttaaactg gcgggatagc catttatcga ccagcttcat cagcagcggc
     8281 gtggtcatca tggaaagcgt caccgtcacc agcaacagcg ccatctggtc gccctggaat
     8341 aagcgttgtg aagaagcggt agaaaagagg acaaaggcaa actcaccgcc ctgactcaac
     8401 acgccagcaa actgcatccg ctcagaactg cgcacgccat acaatcgcgc cagcagatac
     8461 agcacgagaa ttttcaccgc caccagcaca accacactta tcactaccca caacagatgg
     8521 gtataaagca ccccgaggtt gagcgacatg ccgacagaga taaagaacaa accaagcagc
     8581 aagcctttga agggatcgat agccgtttcc agttcatggc gatattcact ttccgccagt
     8641 agcacacccg caataaacgt accgagcgcc atcgacaacc ccagcgcatc cataaataac
     8701 gcggaaccca acaccagcag cagcgtcgcg gcggtgaaca cttcccgcac accagaagct
     8761 gcaataaaac ggaataccgg acgcagtaaa tagcgcccgc caatcagcat gccgacaaac
     8821 gccagcacct tcatgccgat cttcatccag tcgaaatgtt cgtctgccga acccgccaac
     8881 aacggcacta acgccagtgc cgggatcacc gccagatcct gaaacagcag taccgagaaa
     8941 cctaactggc cggattcgct gcgattcatc cctttctcac gcatcaattg caacgccatt
     9001 gcagttgaag acatcgcaag gccaatgcca ccgaccaccg ccgcctgcca tgagaaatgg
     9061 gtcagcatca ataatcctgc cagcaacgcc gcgcttaaca gcacctgtgc cgcgcctacg
     9121 ccaaaaatcg aacgccgcag ttgccaaagc ttggagggat tcaactcaag gccgatgata
     9181 aacatcagga ataccacgcc gagttccgaa aagtggagga tctcatcgac gtcgctaata
     9241 aaccccagcc cccacgggcc aatcgcaatc cccgccagca aatatcccaa cactgcacca
     9301 annnnnnnnn ngccgcgaag agaaacagca ctcctgcgag taaaaaatca gaaccttcca
     9361 tcagcggcct cctggagaca acggatttgc cagccagtca ccgtaggctc tggcgtggct
     9421 cgccagctcc tgtgcgcttt gccgtctcgc ccagtaaata atgatggggc ttaaccaatg
     9481 catccggcac atgcccgccg ccagttcaaa ggggcgcaga acatcgctca tcgggtagcg
     9541 attcaacgcg tcataacggt aagcactttc cggctccccg gtggtaatca cgctacgcca
     9601 gtactttccc gccagttggt ttccccccgg tccgctggca aaaccacggc ttaatacccg
     9661 gtccagccac tctttcagta gcgccgggca gctataggta taaagaggat gctgaaagac
     9721 aatcacctcg tgctcgcgca gtaatgcctg ctcacgaggg atatcaataa aaaagtcggg
     9781 atagtgcgcg taaaggtcgt gcacggtaac attgctgagc tgcgtggccg gtttaagcag
     9841 aacccggttt gccaccgagt cctgagattc cggatgggca tacagcagca aaactttcgc
     9901 tggctgagac atcatccccc tcccggtatg gtttttgtgt atagtcgctg ttttgggcta
     9961 ccattgcgcc cggtgcggca gctcgcccat acattacatt atcataatga taagttaaca
    10021 tagtctgaac atacggcgcc ttatgattgt tttttcctcg ttacaaattc gtcgcggcgt
    10081 gcgcgtcctg ctggataatg ccaccgccac catcaacccc gggcagaaag tcggcctggt
    10141 gggtaaaaac ggctgtggta aatcgaccct gctggcattg ctgaaaaatg aaatcagcgc
    10201 cgacggcggc agctacacct ttccgggaag ctggcaactg gcgtgggtga atcaggaaac
    10261 gccggcgtta ccgcaagcgg cgctggaata tgtcattgac ggcgaccgtg aatatcgtca
    10321 actggaagcg cagctacacg acgccaacga acgtaacgac gggcacgcca tcgccactat
    10381 tcatggcaag ctggatgcta ttgacgcatg gagtattcgc tcccgtgccg ccagcctgct
    10441 gcacggcctc ggtttcagca atgaacaact ggagcgcccg gtaagtgatt tctccggtgg
    10501 ctggcgtatg cgtcttaacc ttgcccaggc gctgatttgc cgttcagact tgctgctgct
    10561 cgacgaaccg actaaccacc tcgatctcga tgccgttatc tggctggaaa aatggctgaa
    10621 gagctatcag ggcacgctga tcctgatctc tcacgaccgc gacttcctcg atccgatcgt
    10681 tgataaaatt attcatatcg aacaacaaag catgttcgag tacaccggca actacagttc
    10741 gtttgaagta cagcgcgcca cccgtctggc gcagcaacaa gcgatgtacg aaagccagca
    10801 ggaacgcgta gcgcatctgc aaagttatat cgaccgtttc cgtgccaaag ccaccaaagc
    10861 gaagcaggcc cagagccgta ttaagatgct ggagcgtatg gagctgattg ccccggcgca
    10921 cgtcgacaac ccgttccgct ttagcttccg cgcgccggaa agcctgccta atccgttatt
    10981 aaagatggaa aaagtaagcg caggctatgg tgatcgcatt attctcgact cgattaaact
    11041 gaatctggtc cccggctcgc gcattggtct gctaggccgc aacggcgcgg gtaaatcgac
    11101 attaatcaaa ctgttagccg gggaacttgc gccagtcagc ggtgaaattg gtctggcgaa
    11161 agggatcaag ctcggctact tcgcccagnn nnnnnnnnaa aaagcccgcc tggtgctggc
    11221 attaattgtc tggcagcgtc cgaatctgct gctgctcgac gaaccgacca accaccttga
    11281 cctcgacatg catcaggcac tcaccgaagc attaatcgag ttcgaaggcg cgctggttgt
    11341 cgtctcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    11401 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggt tgagcgacgt
    11461 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aacgcgaaca gcgcccaggc
    11521 acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc cactgcgtaa
    11581 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    11641 agagaaactc ggcgacagcg aactgtacga tcaaaaccgt aaagcggagt tgaccgcctg
    11701 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg catggctgga
    11761 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    11821 acaacgaccg atgccaatga atttagcagc agtgctgaat tcacccctat gcgcggcttt
    11881 agcaattgtc atctacaaac catgctgccg cgtctgttcc gtcgcaaggt gaaattcacc
    11941 ccgtactggc agcggctgga gttgcccgac ggcgattttg tcgatctcgc gtggagtgaa
    12001 gatcctgcac aggcgcggca taaaccgcgt ttagtggtat ttcacgggct ggaaggcagt
    12061 ctcaatagcc cttacgctca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12121 gtggtgatgc attttcgcgg atgcagcggt gaaccaaacc gtatgcaccg catttaccat
    12181 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga gtttggtcat
    12241 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctggca
    12301 aaagaaggca atgatctccc gattgatgcg gcagtgattg tctctgcgcc gtttatgctg
    12361 gaggcctgta gttaccatat ggaaaagggc ttttcccgca tttatcagcg ttacttgctg
    12421 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac actgccgatt
    12481 aatctcacgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    12541 agaattcacg gctacgctga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    12601 ctcaatcgga tagccaaacc gacactgatt attcacgcca aagacgatcc gtttatggat
    12661 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca gctgactgaa
    12721 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    12781 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    12841 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    12901 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    12961 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatatcatgc
    13021 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13081 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13141 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcgcagt taaatctgca
    13201 tgcagccgag gtggaaggcg acagttttca ccgttatacc cgcccggaaa tggacatggc
    13261 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aagcgaacga
    13321 cttcggcctg ctggaacaaa ccttcattga atacggtcag agtggcaaag ggaaatctcg
    13381 caaatatctg catacctacg atgaagccgt accgtggaat caggtaccgg ggacattcac
    13441 cccctggcaa tctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    13501 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    13561 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    13621 acgagaagca gtgatggact cagtagtgcg ctcaatggaa gactatatca actacatcac
    13681 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacctcaaa
    13741 cccgttcgcg gcaaaaggta tcccgtcact ggatgaaagc tttgtggtga tccattttcg
    13801 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    13861 ccacatcaat acgttagtgg tgcctggcgg caaaatgggt ctggcaatgg aattaattat
    13921 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaacgct gctattacgc
    13981 cggataaaat actatccggc ttcacaacgg gatagttaaa tcacgcggca accacttcat
    14041 acgagtgagt aatattcacc gctttttcca gcatcagcgc cactgaacaa tatttctcgg
    14101 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcgcgaccg gtgacgataa
    14161 aatgtagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgctca gaggtcaatt
    14221 ttacttcaca at
//
LOCUS       DAWDRC010000027        13692 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2355_length_13692_cov_2.650216, whole genome shotgun sequence.
ACCESSION   DAWDRC010000027 DAWDRC010000000
VERSION     DAWDRC010000027.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13692)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13692)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_13692_cov_2.650216"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6619..6628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8780..8789
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10518..10527
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3449 a         3535 c         3382 g         3296 t
ORIGIN      
        1 atcaagcaat aaagagagtt catcaaagat gagtgatgaa atttacctga ccataaccgg
       61 agaacaacaa ggatgtattt cctctcgttg cggaacgagc gcatcaatag gtaatcgctg
      121 gcagattggc catgaagatg aaatcttcgc gttctcgcta tctaatagta ttacgaatac
      181 aggcaaaggg tcacaattac atggccttag tttctgtaag ttgattgaca aaagctcccc
      241 gttacttatt aacgctatta acaataacga gcagttgttc atggagtttg acttctatcg
      301 gatcaataga tttggtcgat gggagaaata ttacaacatt caactccgcg gcgcgctctt
      361 atcagcaatc aatcacctgt ttactgagaa caatctggat acagaaacca tcactgtcag
      421 ttatgaatat atactctcca ggcatctgat tgctaatact gaatttagtc atttggcatt
      481 tcccgataac tacaaccgtc tgtttatacc acggcaaaaa actaaagatg ataatggcct
      541 caaaacactc aatagtaaag cggttggcag actgcttgct gctggcggca tatacaatgg
      601 gaatatcgaa ggattcaggg aaacagcaaa caagttggga ggagatgcac ctgctggtta
      661 cgatcaattt atggataaca aagggttact catcgctggt gcttcaatag ttgctggagt
      721 tgttctggga agaatgagat tccctgattt ggaatcattt gagcatttcg gagccagagg
      781 agcagtatca ggaagaccat ttaatcccga cattgccggt ggtccaatag aaaacctgac
      841 gatagatggc gtaacaatca atagggaagg tatcgctatt gttgaaaagc atatctccag
      901 atttgatcat gatccagcta atgatgtaat gatttcaagg ctaaaaaaaa tagcaaataa
      961 agaattgctt cctgaaaaat acgatctgaa ctactatact catgaatgcc gcgagtatca
     1021 gcgttattgt aatttaggat gggaaacagg tgaaccgaat ggtttagatg gatatgaatt
     1081 atggaataat gcacatactg caactctaga agactttaaa attaaagata ctgacttatt
     1141 tcatccggat gcaaaaaaat gacgattacg atagttgaat ttaacgtgct gaaagttatg
     1201 gctgagaaag atattgactg gagctggatg gtgctggatc gtactttagc tatacgaaat
     1261 attccaggat ttggcaacgt agcgaatatc gtaacaaagc tggttaatca tgggttggtt
     1321 gatatcgtaa atggtgaggg caactctaaa ccacgctaca gagtttctca atatggattg
     1381 aatcttatca aagaacaaca agacaacctc ttctgatcca gccattacag gagcatgatt
     1441 tacattgtga tctttcaata agatactcat caattttagt acagaatatc gtgatcacta
     1501 aaaatgaact taacgtgctg aacgttatga ctgagaaaga tataaactgg aactggatga
     1561 atctcgatcg cacactatcc ataaggcaaa taccaggatt cggtaatgtt gttaatattg
     1621 ttaacaaatt agctaacgaa ggtttggtta ttattgagga tagcgggcat aagtctatgc
     1681 ctcactacca cgttagtgaa aagggataca atctcgtgca acgtgagaat aaataacgta
     1741 gcgttgatta ctgaacggaa tcactcaaaa ctgtgttttg cgaaaaaaac gattcggtta
     1801 tttctactcc tgaagatctg cttcaatctg gcactcatgc aaaatggctt ccagtactgg
     1861 aagccattat cgtactgcaa tttacaatga ctcgttaata ctcatcatcc ccgacgcaaa
     1921 ttctgcgccg cctgcaccat gttcgccagt gccgcgcggg tttctggcca gccgcgcgtt
     1981 ttcaggccac agtccgggtt gacccacaga cgctctgccg gaatgcgttt tgccgctttc
     2041 ttcagcaagg cttcaatcca ttccacgctc ggtacgtttg gcgagtgaat gtcatagacg
     2101 ccaggaccga tttcatttgg ataatcaaac tcttcaaacg actccagcaa ctccatgtcg
     2161 gaacgcgagg tttcgatggt gatgacgtct gcgtccagcg ccgcaatcga atccataatg
     2221 tcgttgaact cgcagtaaca catgtgagtg tggatttgtg tgtcatcttt cgccacggcg
     2281 gcgttgatac ggaaagcctc cacgccccac tggagatagg catcccagtc gctgcgacgc
     2341 agtggtaaac cttcgcgcaa tgccggttcg tcaatctgaa tgatgccaat tccagcggct
     2401 tccagatcgg ccacttcatc acgcagtgcc agcgcaatct gtttggcgat ggtttcacgg
     2461 ctgacatctt cacgcgggaa cgaccagcag agaatagtca ccgggccggt caacatccct
     2521 ttcaccggtt tatcagtcag ggattgcgca tattttgccc actccacggt aatcggtgcc
     2581 gggcggctaa cgtcaccaat aacaatcggt ggcttcacgc agcgggaacc gtagctctgt
     2641 acccaaccgt tttgcgtaaa gacaaagcca tccagatgct cgccaaagta ttccaccatg
     2701 tcattacgct cggcctcgcc atgtaccagc acatccagtc ccaaacgttc ctgctcaaca
     2761 atggcctgct tgatatgttc cgcaatgccc gtgcggtagt tattggcgtc gagattaccc
     2821 tttttaaaat ccagacgcag ggtacgaatc tccgtggttt gcgggaagga accaatcgtg
     2881 gtggtcggcc acgcgggcag tttaaaacgc gcacgctgag cttcagcacg cacttcatag
     2941 acattcgcac gctgactgtc ctgggcggtg atcgccgcca gacgcttttc tactgccgga
     3001 ttatgtacac gagtagagtg acgacgcgcc tgaatcggag cgctccactc tgccagagct
     3061 gccgtatcac cactgttcaa cgcatcgcgc agcaatgcca gttcatgaca tttttgcagg
     3121 gcgaaggcaa accagctttt cacttctgca tcaagacgcg tttccacgct caggtcgatg
     3181 gggctgtgca gcaacgagca ggaagatgcc acccacaaat cgcgtttgcc gacaatgtcc
     3241 ttaatttgcg catatttctc ggtaagatcg gcgcgccaga cgttacgacc attgataaga
     3301 cccgcagaca gcagccagtc agaaggcaga cgcttgtgca gttcagcaac gtcatcttta
     3361 ccatgtacaa gatcgacatg cagaccctga acaggcagcg cagtaatcgt gtcgagattt
     3421 ggcgttacgc cttcaaaata ggtggtcagc agcagtttca cctgtccctg gagcgcgtcg
     3481 taagcgggtt tgtatgcgtt cagccacgcc tgcggcagtt ccagtaccaa cgcgggttca
     3541 tcaatctgta cccactcgat gccgcgtttt gccagttctg ccagcacttg ctgataaacc
     3601 ggcagaatgt cgttcagcag gctcaggcga tcaaactgtt cacctttcac tttacccagc
     3661 cacaggtagg taaccggccc cagcagcacg ggcttgacct tgtggcccag cgccagcgcc
     3721 tcgtccactt cctccagcag ctgcgtccag gtcagtttga actgttggcc tttaacgaac
     3781 tccggcacca tgtagtgata gttggtgtta aaccatttgg tcatttccgc tgccgccgca
     3841 ggttcgccag tcggtgcacg tccacgacca atacggaaca gggtgtcgat atctaccgaa
     3901 ccatctttgt tctggtgacg cgccggaacg ttacccagca gcaggctggt ggtcaggaca
     3961 tgatcgtacc aggcaaaatc acccaccggc agcaggtcga tacctgcttg cttttgttga
     4021 tcccagtgac gagcacgcaa ttcacgccct accgccagca gttcttcacg cgtggagttc
     4081 cccgcccaat aactctcttg cgcttttttc agctcgcgac gcaggccaac gcgagggaaa
     4141 ccgagggtgt gattaagaat tgtcattata tgccccttat ggaattttta gtatttagcc
     4201 gtccagatgt ttacacatcc ataattggcg gttactgtat attcctcaag cgcaaattat
     4261 tcatgccgaa gtgaaggact ttcatgatcg aagtaaaaca cctgaaaacg ctacaagcgt
     4321 tgcggaactg cggctcactc gcagccgctg cggcgacgtt gcatcaaacg caatccgccc
     4381 tgtctcacca gtttagcgat ctggaacaac gccttggctt ccggctattt gtgcgtaaaa
     4441 gccagccgct tcgctttaca ccgcagggag aaatcctgtt gcaactggca aatcaggtgc
     4501 tgccgcaaat tagccaggca ctgcaagcct gcaatgaacc gcagcagacg cgcctgcgca
     4561 ttgccataga gtgccatagc tgtattcagt ggctgacgcc cgcattagaa aatttccata
     4621 agaactggcc gcaggtggag atggatttta aatcgggcgt aacgtttgat ccgcagcctg
     4681 cgttgcaaca gggcgagctg gatctggtga tgacgtccga tattctgccg cgcagtggcc
     4741 tgcattattc gccgatgttc gactatgaag tacgtctggt gttagcacct gaccatccac
     4801 tggcggcaaa aacgcgtatt acaccggaag atctcgccag cgaaacgctg ttgatttatc
     4861 cggtgcagcg tagccgactg gatgtctggc ggcattttct tcagccagca ggcgtcagcc
     4921 cgtcactgaa aagcgtcgat aacaccttat tgttgattca gatggttgcc gcacggatgg
     4981 gtattgccgc actacctcat tgggtggtgg agagttttga acgccagggt ctggtggtca
     5041 ccaaaaccct gggcgaaggc ttgtggagcc gactgtacgc cgccgtgcgc gatggcgagc
     5101 agcgtcagcc gattacagaa gcgtttattc gttcagcgcg caatcacgcc tgcgatcatc
     5161 tgccgtttgt gaagagcgcg gagcgaccca cttacgatgc acccacagtg aggccaggat
     5221 caccagcgcg cctgtaataa acgttggcca gtgcggctgt tggtgccaga tagccaggtt
     5281 taccagcagc cctgccggaa cgtgcatatt attcataatg cccagcgttc cggcgtccac
     5341 ctgcgtcgcg ccgtagttcc acataaagta gccaatcccg gaagccacca cgccaagaaa
     5401 caccagaatg ccccattgca acgtggtttg cggcattttc tgcgcatttc ccagcaagaa
     5461 ccatgcaatc actgccacca ggaacgcgcc aagatagaac cacgcaaagg cgttatgctg
     5521 cggcatcggg cgagtttcca tcagacgttt gtaacccacc atgccgatgg caaaagtgat
     5581 attggagagc tgcaccagca acaagccagt ccagaaatgg tcggtgacct gatcatagcg
     5641 aataatcccg gcaccaatca ccgccaacaa ggcgctaaaa gcatagcccc agcgcagacg
     5701 gcgcttactc atgatgtcat aaatcagcgt gatgtagagc ggcgtcagta cggtgaacag
     5761 caacagttcg gagaccgtca ggtagagata ggcgcggaaa ctcagcatat acatcacacc
     5821 gagctgcatc gcgcccacca gcatatacag gccaaccgtt ttcaggctat tgccacgggt
     5881 gcgcagaaac ggcagaaaaa cgagtgccgc cagaccaacg cgcaccagca ccgcaaaata
     5941 gctatcgacg tgccccgcaa ggtactcgcc ataaaagcta aaggagaagg cccacagaat
     6001 cgtggtgatg ataagtagcg ccacaatgga aaactctttg attaacgagt atccattgta
     6061 gcgaagtatc aggttgacaa ctgaccaaat aaagaacgat tacgataaat agagcttacg
     6121 cagataatgc ggcactgcgt cctcggcgtt agtaccaatc acttccagct ctggatgcag
     6181 atctttcagc cgttgatgcg cgctgcccat aatgcagcct ttccccgcca tcgacagcat
     6241 ttcggcatcg ttcattccat cgccaaaggc aatacaatct tgcaggctgt agcccagttt
     6301 cttcgctacc gcttccaggg catggccttt tgaaacgccg ccagccatga cttccaggca
     6361 ggttaaggta gagaaactga cattgacgcg atcgccccag cgagcgttaa tcgcttgctc
     6421 cagtggcaga agtttttcat gggaatcgca ggtgaagaac actttgctga cgccttccgg
     6481 ctccagcaat cccggctcat acagcgcata tttgaacacc gcttctttaa aaaagcgcat
     6541 ctcttccggg cgatggcgat tcataaacca ttcgtcgtcg cgataaacgt tagtaatgat
     6601 gtccggattg tcgttgacnn nnnnnnnnaa acagatcact ggcaatgtcg cgatccaggt
     6661 tatgagcaaa aatcaggttg ccatcgaggt cgtgaacgcg cgcgccattg gaggtaatca
     6721 tgtaagactt aatctccaga ttatcgcgaa tttgccccac atcaacgtgg tgacgaccgg
     6781 tcgcaaacac aaagttgatg ccgcgcgcgg tgagcagctt cagagtttct ttggcgtaag
     6841 gggataacgt atggtcggga gaaagtaacg tgccatctaa atcagacgca acaacctggt
     6901 acataagaaa tttaaacctc tgtagagcgg tttccgctgg gtgagttatg cctgttgaaa
     6961 aaatcaacga tagcgtggag cgcgactgag cgcattgcgt ccttttcaaa aaggatctca
     7021 tggtacgcac ctttaattac caacggccgt cctccttcga caggatggcc cgcagcggtg
     7081 cgtagttcac aaaaacggtc gtgcatgcgg ttatccacca cgcgttcctc ttcagcttgt
     7141 aagagaagcg ttggcgtggc gtcatcaccc gcaccggcta acacctgttc gccagccaga
     7201 atactttcgc gtacccaatg gtaggtcggc ccaccgacgc gaatcgttgg gtcatcagca
     7261 tagaaacgta agttacgtcg atatcgctgt ctgctgtggg tcagtacgtt gatagcaaac
     7321 ggcaacgcgc gccagcgccc ggtgcctatc gcatagccat cacggaaacg tggatgcgct
     7381 tcggcccagt taaggatctg ccgtgccata aatgacggca tacgaatcac aatcccaaac
     7441 attggcgcag ttagcgcaat ggcgtcacat acacctggat ggcgttgcag aaataatgtg
     7501 gagatcgcac cgcccatcga atgtgccagt atatagcgtt tacgccacgg accgggctga
     7561 acctcctgtt gccagaatgc cgccagatca tcaacataat cattaaagcg attaacatgc
     7621 ccgagatgtg gatcggctaa caggcgaccg gaacgtccct gcccgcgatg gtcgatgatt
     7681 aaaacatcaa accccaaatg gaacaggtcg taggccagtt ccgcatattt tacgtagctc
     7741 tcaatacgcc ccggacagat gactaccacc cggtcatggt gctgtgcgcg aaaacggaca
     7801 aagcgaaccg gaatgtcatc cacaccagta aactctgctt catcacgctg acgccagaaa
     7861 tcagtcagcg gtcccatggt aaaagcagca aacgcgtttt ctcttgtttc ccagtctttt
     7921 tgctgctgaa acatcgggta atctgcctct taaaccacgt aaaatcgttt ttttaagcgt
     7981 gcctgacaca acgctgcgac agtagcgtat tgtggcacaa aaatagacac accgggagtt
     8041 catcatgacc ttagaatggt ggtttgccta cctgctgaca tcgatcattt taagcctgtc
     8101 gccaggctct ggcgcaatca acaccatgac cacctcgctc aaccacggtt atcgcggcgc
     8161 ggtggcgtct attgctgggc ttcagaccgg actggcgatt catattgtgc tggttggcgt
     8221 ggggttgggg acgctatttt cccgctcagt gattgcgttt gaagtgttga agtgggcagg
     8281 cgcagcttac ttgatttggc ttgggatcca gcagtggcgc gccgctggcg caattgatct
     8341 taaatcgctg gcctctactc aatcgcgccg acatttgttc cagcgcgcag tttttgtcaa
     8401 tctcaccaat cccaaaagta ttgtgtttct ggcggcgcta tttccgcaat tcatcatgcc
     8461 gcaacagccg cagctgatgc agtatattgt gctcggcgtc accactattg tggtcgatat
     8521 tattgtgatg atcggttacg ccacccttgc tcaacggatt gctctatgga ttaaaggacc
     8581 aaagcagatg aaggcgctga ataagatttt cggctcgtta tttatgctgg tgggagcgct
     8641 gttagcatcg gcgaggcatg cgtgaaaata atgtcggatg cggcgtaaac gccttatccg
     8701 acttactctg atgacgcgtc tggcattaac gcgaaataat caaatgaatg ccaaatccgg
     8761 caaataacgc cccggcaaan nnnnnnnnnt agataatcgc tttaggattc gcgagattgg
     8821 tcagtaagcc cttaagaaaa ctgcgcccgc ttttcgccag ttcgacttgt ggcgcagggg
     8881 cagaaacaac ctcttttttc agtgcaccgc gtagcatctg gtaacccatc cagcagagat
     8941 acaggccacc gcccaccata atcagcgtat gcagccaggc cattttttcg ataatcaaat
     9001 gtaggccaag caacgcaatc ccagcccaaa ccattacgcc gcaggtaatg cccagcacgc
     9061 ccatcatcgc ttctttacgg gaacgactaa cagcggtctg agagacaaaa aagaaatcgg
     9121 gaccggggct cataagcgcc acaatgtgca ccatggcgac ggtgaggaat aacatcaaca
     9181 tacattagac tcgcggggga aacgtttacg gagtcttcat cctggcactt ttttatgctg
     9241 ctgactactc ttcgtcgtca ccatcgacat gcgcacgaat cagcgccata aacggtttgc
     9301 caaagcgttc cagcttgcgc atcccaacgc cgttaacgct gagcatttcg ctggcggtga
     9361 tcggcatctg ttcagccatc tcaatcaagg tagcgtcgtt aaacaccacg tacggcggga
     9421 cattgctttc atcggcaatc gatttacgca gtttgcgtaa tttggcgaac agtttgcgat
     9481 catagttgcc gccgaacgat ttctgcatcg ctttcggttt gagcgccacg atacgcggca
     9541 cggcaagttg caaagaggat tcgccgcgca gcaccgggcg cgcagcctct gtcagttgta
     9601 gggcagaatg ctgggcaata ttttgcgtca ccaggccgag gtgaatcagc tggcggatca
     9661 cgctcaccca atgttcatgg cttttatcac ggcccatgcc atagactttc agtttgtcat
     9721 gaccatagtc gcggatacgc tgattattag caccgcgaat cacttccacc acataaccca
     9781 tcccaaaccg ctgattcaca cgaccaatgg tggaaagggc aatctgcgca tcggttgaac
     9841 catcgtactg ttttggcgga tcgaggcaga tatcgcagtt cccgcacggc tcctgacgcc
     9901 cttcgccaaa atagttcagc aataccaaac gacgacaagt ttgcgcttcg gcaaacgcgc
     9961 ccatcgcatt gagtttatga cgttcgatat cctgcaactg cccctgcggc ttctcttcca
    10021 gacaacggcg cagccacgcc atatcagccg gatcgtaaaa cagcatcgct tccgcgggca
    10081 ggccatcacg cccggcgcgt ccagtttcct gataatagga ttcgatattg cgcggaatat
    10141 caaaatgcac cacgaagcga acgttaggct tgttgatgcc catgccgaac gccaccgtcg
    10201 ccaccacaat ttgcaggtca tcgcgctgga atttttcctg cacatcagcg cgaacattat
    10261 tttccagccc ggcatgatag gccgccgcgc taattccctt gctttgcagg cgcgcagcgg
    10321 tgtcttctac tttcgcacga ctattacagt aaataatccc cgacttaccg cgttgttcct
    10381 gtacataacg tatcaactga tcgagcggtt tgaatttctc catcagcatg tagcgaatat
    10441 tcggacggtc aaaactgctg atttgaatca gcggatcgtt cagccccagc aggcgcacga
    10501 tatcctcccg cacctcgnnn nnnnnnngct gaggcagtca gcgccataaa cgggagcgtc
    10561 ggaaaacgct gacgcagctg accgagggca gcgtattccg ggcggaaatc gtgaccccat
    10621 tgggagatac agtgcgcttc atcaacggct aataacaccg gattccagtg cgccagatgc
    10681 tcaagaaagt tatccagcat caggcgttcc ggcgcgatat aaagcaggct gatttgcccg
    10741 gtgcgacagc ctgtcatcac ttcaagttgc tgctcgcggg tttgcgtcga gttaaggcac
    10801 gccgccgcca cgccgttggc ttgcagttga tctacctgat ctttcatcaa cgaaatcagc
    10861 ggtgagacaa ccacggtaag gccgtttagc aataaggcag ggatttgata gcaaagggat
    10921 tttccgccac cagtgggcat gacgaccagg caatcgcgac cggaaagtac ggtgtcgata
    10981 atctcttcct ggccggggcg aaactgttgg tagccaaagg tttcttgtaa aacctgttta
    11041 gccccggact ccagattcaa cacttccgcc tgcgccacat tcaccccgat tgcctgaaat
    11101 aaaaacaggc gctattttca gcgcctgcgc gagaaactgc aatgctttta gtctctgaga
    11161 gggtaatcct ggatgctgga ttccagattt gccaaccatt catcaaaaca ggtcgtttag
    11221 cataactccc acaccaacgc gggtctggtt gaagttatag tcgatgagcg attcgccgta
    11281 accgctgtaa atctgagtat aaaggcgcac atgtttggtg atcgggtagc ttaagcctaa
    11341 ctccgcgccg ccgtaaccgg tgttccagtt gtactgccct ttcgcgctca gtaccgcatc
    11401 accgaggtga tagccgattt taagctggta gtaacccata tatttggtga tgtccgggtt
    11461 atcgtccgta ttccccacca cataccacgg cttcacctct accagccagt taccgttttc
    11521 tgccatcagg cgagtataaa ggcggttcca gctgcgggaa gtcgggtctg aacgcccgtt
    11581 tgagtcgtgg ttgtagccca tttccacatc gcgcagcgtc cagccagcaa agttgtaatc
    11641 cgtggcaaaa ccgaggaaca attgcggttc gtagttagtt tcacgaaacg gtgatgactc
    11701 atcgctatta gaaagctgcc accaggattt ctgtgtatag gaggcgccca gtacagagtt
    11761 tggccccaaa atcccacgcc acagcggaaa cgccaggctc aactggaact ttacttcatc
    11821 cttacgcgcg ttttccgccc agtcgtaact ggcaatcgct tctttattca gatcgctggt
    11881 ttgggtgtag atgatgtagt tggtgtcata aggataaagg gtgaacggat tgtcatgctc
    11941 ctgcagcata ttggcgataa tactgccacg caccgctggc gcgtcatgca cctctttcac
    12001 cgttgcctct tgtgcatata ctgccatagg caacataaac actggcaaca accagccctg
    12061 cagagtccgc atcgctcgtg ttctccgtaa cgaattattg ataaggtgaa tatttttctg
    12121 cgggctattt tacatactta actccatagc cgttagttat cccttcctaa agtagaaacc
    12181 aacaataaat aagcataaaa tcaacatttc attaactaat gaagtgtaag gatgttatgt
    12241 ctgccgtact gaccgctgaa caagccctca aattagtggg tgagatgttt gtttatcaca
    12301 tgccatttaa ccgcgcattg gggatggaac tggagcgtta cgaaaaagag ttcgcacagc
    12361 tggcctttaa aaatcagccg atgatggtgg gtaactgggc gcaaagcatt ttgcacggcg
    12421 gggtcattgc gtcggcgctg gatgtcgccg caggtctggt gtgcgtggga agtaccttaa
    12481 cccgccacga aaccatcagt gaagatgaac tacgccagcg gctatcgcgg atggggacca
    12541 ttgatcttcg cgtcgattat ctgcgcccag gcagaggcga gcgttttact gctaccagta
    12601 gcctgttgcg tgcaggcaat aaagtcgccg tcgcccgcgt tgaattacac aatgaagaac
    12661 agctttatat tgccagtgcc accgccacct atatggtagg ttgaagacat aaaagcaggt
    12721 aaactgctgt taccaattaa aaatgagtat tcccgatgga tgcaaaacaa acgcggcagg
    12781 gcgtattact cgctcttgcc gcttatttta tttggggtat tgcgccagcg tacttcaaac
    12841 tgatttacta cgtgcccgcc gatgagatcc tgacgcatcg cgtgatctgg tcgttcttct
    12901 ttatggtggt gctgatgagc atttgccgcc agtggtccta tttaaaaacg ctgattcaga
    12961 cgccacagaa aatttttatg ctggcagtct ctgccgtgct gattggcggc aactggctac
    13021 tgtttatctg ggcggtgaac aatcaccata tgctggaagc gagccttggt tactttatta
    13081 acccgctggt gaacattgtg ctggggatga ttttcctcgg cgagcgattc cgccggatgc
    13141 aatggctggc ggtgattctg gcgatatgtg gagtgctggt tcagttgtgg acttttggtt
    13201 cactgccgat catcgcgctg gggctggcgt ttagttttgc tttctacggc ctggtgcgca
    13261 agaagattgc tgttgaagcg caaaccggca tgttaatcga aactatgtgg ttgctgcccg
    13321 tggcggcgat ttacctgttt gctattgccg acagctcaac cagtcatatg gggcaaaacc
    13381 cgatgtcgct gaatttactg ctgatcgccg ccggtattgt caccaccgtg ccactgttgt
    13441 gttttactgc agctgcgaca cgcctgcgtc tctcaacgtt aggctttttc cagtacattg
    13501 gtccgacgct gatgttcctg ctggctgtga cgttttatgg tgaaaaaccg ggtgccgata
    13561 agatggtaac tttcgccttt atttgggtgg cgctggcaat ttttgtgatg gatgcgattt
    13621 atacgcagcg tagaacgtcc aaataaaaaa taaatatatt ctgaaaaaat atgccgactc
    13681 ttgcatgaga gt
//
LOCUS       DAWDRC010000028        13668 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2356_length_13668_cov_2.576655, whole genome shotgun sequence.
ACCESSION   DAWDRC010000028 DAWDRC010000000
VERSION     DAWDRC010000028.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13668)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13668)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2356_length_13668_cov_2.576655"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3261..3270
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5640..5649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3202 a         3560 c         3618 g         3268 t
ORIGIN      
        1 tatgaatgcg gtggctgaat aaagctattt ttattcatcg agaaaatagc catactgacg
       61 ccttcaatgg atattgatat tcactacaga tattgttcgc agcagacagg atgtatttaa
      121 tatgccaaca gttctctctc gcatggcgat gcaactcaaa aaaacagcct ggataattcc
      181 cgtcttcacg gtttcgggat gctcattatc tccggcaatc ccggtgatcg gcgcttatta
      241 tcccggctgg tttttctgcg ctattgccag ccttattttg acgctcatca cgaggcgaat
      301 tattcagcgg acaaatatca atctggcatt tgtcggaatt atttataccg ccctttttgc
      361 tctctacgcc atgctgttct ggctggcatt tttctaatct atttgagacg cgctcatgga
      421 aagtacgccg aaaaaagctc ctcgcagtaa attccctgct ctgttagtgg ttgcgttggc
      481 gctggttgcc cttgttttcg ttatctggcg cgtagacagt gcgccatcaa ctaatgacgc
      541 ttacgcgtca gcagatacca ttgatgtggt gccggaagtc agcggtcgca ttgtagaact
      601 ggcggtcacc gacaaccagg cggtgaaaca gggcgatttg ctgttccgca ttgacccgcg
      661 cccgtacgaa gccaatctgg cgaaagctga agcctccctc gcggcgctgg ataagcaaat
      721 tatgctcacc cagcgtagcg ttgacgcaca acagtttggt gccgactcgg ttaatgccac
      781 ggtagaaaaa gcccgtgccg ccgcgaaaca agccagcgat acgttacgcc gtaccgaacc
      841 gctactgcga gaaggttttg tttctgcgga agaggttgac cgtgcaagaa ccgcacagcg
      901 tgcggcagaa gcggacctta atgccgtatt gttgcaggcg cagtcagccg ccagcgccgt
      961 cagcggcgtg gatgcgctgg tggcgcagcg tgcggcagtg gaagcggata ttgccctgac
     1021 caaactgcat ctggaaatgg cgaccgttcg cgcgccgttt gatggccggg tcatttccct
     1081 caaaacctcc gtcgggcagt ttgcatctgc gatgcgccct attttcactc taatcgacgc
     1141 tcgtcactgg tacgtgattg ccaacttccg cgaaactgat ctgaaaaata ttcgctcggg
     1201 tacactcgca accattcgcc tgatgagcga cagcggcaaa accttcgagg gtaaagtgga
     1261 ttcgattggc tacggcgtgc taccggatga cggcggcctg gtgctgggcg gcctgccgaa
     1321 agtgtctcgt tctattaact gggtccgcgt tgcccagcgt tttccggtca aaatcatggt
     1381 tgataaacct gacccggaaa tgttccgcat cggcgcttcg gcagtcgcta atcttgagcc
     1441 gcaataatga gcgcgctcaa ctccctgcca ttaccggtgg tcaggctgct ggcgttcttt
     1501 catgaagagt taagcgagcg gcgaccaggg cgcgtgccac agacgatgca actctgggta
     1561 ggttgcctgc tggtgattct gatctcgatg acttttgaga tcccttttgt ggcgttatca
     1621 ctggcagtgc tgttttacgg tattcagtcg aacgcgtttt acaccaaatt tgtcgcgatc
     1681 ttgtttgtgg ttgccacggt gctggagatc gccagcctgt ttttgatcta caaatggtca
     1741 tatggcgaac cgttgatccg attgatcatc gccgggccga tcctgatggg ctgcatgttt
     1801 ttgatgcgca cccatcggct gggactggtc tttttcgccg tcgccattgt cgctatttac
     1861 gggcaaacct tccccgctat gctcgactat ccggaagcgg tcgtgcgctt aacgctgtgg
     1921 tgtatcgttg ttggcctcta tccaaccttg ctgatgacgt taatcggcgt gctgtggttt
     1981 cccagtcgtg ccattacgca gatgcatcag gcgcttaatg atcggcttga tgatgccatt
     2041 agccacctga cggacagcct cgcaccgcta cccgaaacgc ggattgaaag agaggcgctg
     2101 gcgctgcaaa aactcaatgt cttttgcctc gcggacgatg ccaactggcg aatccagagc
     2161 gcatggtggc aaagctgcgt ggcaacggta acctacattt actcgacgct aaatcgctac
     2221 gatcccacct cttttgctga ttctcaggca attattgaat tccgacaaaa attagcttca
     2281 gaaatcaaca atctgcagca ttccattacc gaaggtcagt gctggcaaag cgactggcgg
     2341 atcagtgaaa gtgaagcgat ggcggcacgg gaatgtaacc tggagaatat ctgccagacg
     2401 ctgttacagc ttggtcagat ggacccgaat acgccgccaa cgcccgcagc caaaccgcca
     2461 tcaatggtcg ccgatgcttt taccaatcca gactatatgc gctacgcggt aaaaacgctg
     2521 ctcgcctgtt tgatctgtta caccttctac agcggcgtgg actgggaagg cattcacacc
     2581 tgtatgctga cctgcgtgat cgtcgctaat ccaaatgtcg gttcgtcgta ccagaagatg
     2641 gtgctgcgtt ttggtggggc cttttgcggc gcgattctgg cgctgttatt cacgctactg
     2701 gtcatgccct ggctggacaa tattgtcgaa ttgctgtttg tgctggcacc gattttcctg
     2761 ttgggcgcat ggattgccac cagctctgaa cgctcttctt atatcggcac acagatggtg
     2821 gtcaccttcg cgctcgccac gctcgaaaac gtttttggcc cggtgtacga cctggtggaa
     2881 attcgcgatc gcgccctggg tatcatcatt ggtaccgtgg tgtcggcggt gatttacacc
     2941 tttgtctggc ctgaaagcga agcgcgcacg ctaccgcaaa aactggctgg cgcgctgggg
     3001 atgttaagta aagtaatgcg gatcccacgc cagcaggaag tcactgctct gcgcacttat
     3061 ctgcaaattc gcataggtct gcatgcggcg tttaatgcct gtgaagagat gtgccaacgc
     3121 gtggcgctgg agcgtcaact ggacagcgaa gaacgcgctt tactgattga acgttcgcaa
     3181 acggttattc gtcagggccg cgatattctt cacgcctggg atgcaacctg gaactcggcg
     3241 caggcgctgg ataacgcact nnnnnnnnnn cctgccgacc ttattaaaac aggagcaaca
     3301 cgtctgccag cttttcgccc gcttgccaga ctggacagcc ccggcattaa cgcccgccac
     3361 ggaacaggca caaggagcca cgcaatgatc aatcgtcaac tttcacgcct gctgttgtgc
     3421 agcattctcg gcagcacgac gttgatttcc ggctgtgccc tggtacgtaa ggattctgcg
     3481 cctcatcaac agctcaaacc ggaacaaatc aaactggccg atgatattca tcttgccagc
     3541 tccggctggc cgcaggcgca gtggtggaaa caatttaacg acccgcagct ggatgcgctg
     3601 atccaacgga cgctaagtgg ttcacacccc ctcgccgaag cgaaactgcg ggaagaaaaa
     3661 gcgcagtccc aggccgattt gttagatgcc ggttcacaat tacaggtcgc agcgttaggg
     3721 atgctcaacc gccaacgcgt ctcggcgaac ggctttttaa gcccttatgc gatggatgcg
     3781 ccagcactgg gtatggacgg gccgtactat acggaagcca cagtcggttt gtttgccgga
     3841 ctggatctcg atttgtgggg tgtgcatcgc tcagcggttg ctgccgccat tggcgcgcat
     3901 aatgccgcgc tggcagaaac cgcagcagta gagctatcgc tgaccacggg cgtagcgcag
     3961 ctttattaca gtatgcaggc cagctatcag atgctcgatc tgttagaaca aactcgcgat
     4021 gtgattgatt acgcggtgaa agcgcaccag agtaaagtgg cgcacggtct ggaagcgcaa
     4081 gtgcctttcc acggcgcgcg ggcacagatt ctggcggtcg ataaacaaat tgctgccgtc
     4141 aaagggcaaa tcactgaaac gcgagaatct ctgcgtgcat tgattggcgc gggagccagc
     4201 gatatgccgg agatcaaacc ggtggcatta ccacgagtac agaccggcat tccggcaacg
     4261 ctctcttatg agttgctcgc cagacgcccg gatctgcaag ccatgcgctg gtatgttcag
     4321 gcgtcattag atcaggtgga ttccgcgcgg gcgctgttct acccgagctt tgatatcaaa
     4381 gcgtttttcg gtctggactc catccacctg gataccttat tcaaaaaaac cagtcgccag
     4441 ttcaacttca tcccgggtct gaaattgccg ctgtttgacg gtggacggtt gaatgccaat
     4501 ctcgaaggca ctcgcgccgc cagcaacatg atgattgaac gttacaacca gtcagtactg
     4561 aacgcggtgc gtgacgttgc cgtcaacggc acacgtctgc aaacgctcaa cgacgagcga
     4621 gagatgcagg ctgaacgcgt agaagcaacg cgcttcaccc agcgcgcagc cgaggccgcc
     4681 tatcagcgcg gcttaaccag ccgcttacag gccaccgaag cccggttgcc agtgcttgcc
     4741 gaggagatgt cattactgat gctggacagc cgccgggtga tccaaagcat tcagttgatg
     4801 aaatcgctgg gcggcgggta tcagacggct cccgtcgtcg agaaaaaata aaatgtctac
     4861 cgcgtgatgg ctgtcacgcg gtatttcgtt tcgtcacgtc aaaactgacg acagcctgtt
     4921 tttcgtcaga gttttgaata aatagtgccc gtaatatcag ggaatgaccc caaataaaat
     4981 gtggcataaa agatgcatac tgtagtcgag agcgcgtatg cgtgatttga ttaactggag
     5041 cgagaccgat gaaaaaagtc gtcacggttt gcccctattg cgcatcaggt tgcaaaatca
     5101 acctggtcgt cgataacggc aaaatcgtcc gggcggaggc agcgcagggt aaaaccaacc
     5161 agggtaccct gtgtctgaag ggttattatg gctgggactt cattaacgat acccagatcc
     5221 tgaccccgcg cctgaaaacc cccatgatcc gtcgccagcg tggcggcaaa ctcgaacctg
     5281 tttcctggga tgaggcactg aattacgttg ccgagcgcct gagcgccatc aaagagaagt
     5341 acggtccgga tgccatccag acgaccggct cctcgcgcgg tacgggtaac gaaaccaact
     5401 atgtaatgca aaaatttgcg cgcgccgtta ttggtaccaa taacgttgac tgctgcgctc
     5461 gtgtctgaca cggcccatcg gttgcaggtc tgcaccaatc ggtcggtaat ggcgcaatga
     5521 gcaatgctat taacgaaatt gataataccg atttagtgtt cgttttcggg tataacccgg
     5581 cggattccca cccaatcgtg gcgaatcacg taattaacgc taaacgtaac ggggcgaaan
     5641 nnnnnnnnna aaattatcgt ctgcgatccg cgtaaaattg aaaccgcgcg cattgctgac
     5701 atgcacattg cattgaaaaa cggctcgaac atcgcgctgt tgaatgcgat gggccatgtc
     5761 attattgaag aaaatctgta cgacaaagcg ttcgtcgctt cccgtacaga aggctttgaa
     5821 gagtatcgta aaatcgttga aggctacacg ccggagtcgg ttgaagatat caccggcgtc
     5881 agcgccagtg agattcgtca ggcggcgcgg atgtatgccc aggcggaaag cgccgccatc
     5941 ctgtggggca tgggggtaac ccagttctat cagggcgtgg aaaccgtgcg ttctctgacc
     6001 agcctcgcga tgctgaccgg taacctcggt aagccacatg cgggtgtaaa cccggttcgt
     6061 ggtcagaaca acgtgcaggg tgcctgcgat atgggcgcgc tgccggatac gtatccgggc
     6121 tatcagtacg tgaaagatcc ggctaaccgc gagaaatttg ccaaagcctg gggcgtagaa
     6181 agcctgcctg ctcataccgg ttatcgtatc agcgagctgc cgcaccgcgt ggcgcatggt
     6241 gaagtgagag cggcgtacat tatgggcgaa gatccgctgc aaactgacgc ggaactgtcg
     6301 gcagtacgta aagcctttga agatctggaa ctggttatcg ttcaggacat ctttatgacc
     6361 aaaaccgcgt cggcggcgga tgtcatttta ccgtcaacgt cgtggggcga gcatgaaggc
     6421 gtgtttactg cggctgaccg tggcttccag cgtttcttca aggcggttga gccgaagtgg
     6481 gatctgaaaa cggactggca aatcatcagt gaaattgcca ctcgtatggg ttatccgatg
     6541 cactacaaca acacccagga gatctgggac gagttgcgtc atctgtgccc ggatttctac
     6601 ggtgcgactt acgagaaaat gggcgaactg ggcttcattc agtggccttg ccgcgatact
     6661 tcagatgccg atcaggggac ttcttatctg tttaaagaga agtttgatac cccgaacggt
     6721 ctggcgcagt tcttcacctg cgactgggta gcgccaatcg acaaactcac cgacgaatac
     6781 ccgatggtac tgtcaacggt gcgtgaagtt ggtcactact cttgccgttc gatgaccggt
     6841 aactgtgcgg cactggcggc gctggctgat gaacctggct acgcacaaat caataccgaa
     6901 gacgccaaac gtctgggtat tgaagatgag gcattggttt gggtgcactc gcgtaaaggc
     6961 aaaattatca cccgtgcgca ggtcagcgat cgtccgaaca aaggggcgat ttacatgacc
     7021 taccagtggt ggattggtgc ctgtaacgag ctggtgaccg aaaacttaag cccgattacg
     7081 aaaacgccgg aatacaaata ctgcgccgtg cgcgtcgagc cgatcgccga tcagcgcgcc
     7141 gccgagcagt acgtgattga cgagtacaac aagttgaaaa ctcgcctgcg cgaagcggca
     7201 ctggcgtaat accgcgtaaa ctcacgttaa aagcggcgat attacccaac gcctttgtat
     7261 caatccggaa acgactctct gtttccggat ttttttatac atacctgcgc agtcaaacca
     7321 gcccatcctg gttattagcg acgtcttctc ggttgttctt ctattccagt cacgattgca
     7381 aatgagaatt atctttactc aattctcatc gcggcctaac gtaataatac tttacatttg
     7441 ataaacaaaa tttcggtaag gtgagaaatc atcgaccatt taactcgttc tattaacggg
     7501 atattctggc gcgattaatg cagcgaaacc ccgtctgata gcatattaat aacctcaaag
     7561 taatttcagg gattgggaag catatgatta aaaaactatt acctttactg gttttaagca
     7621 caatttccgc cacaaccgtt gccgctacac cgcctaatac gttggttgta gcccaggggc
     7681 tggatgatat tgtgagtctg gacccggcag aagctaatga actctcaagt attcagactg
     7741 taccaagtct gtatcaacgc gtagtacaac cggaccgtaa tgatccgacg aaaatcactc
     7801 cgattctggc ggaaagttgg caggctgacc ccgcagccaa aacgctaacc ataaaactga
     7861 aaagcgatgc gaaatttgcc tctggtaatc cggtacgccc ggaagatgtg atttactctt
     7921 atgtccgggc tgtcacactg aataaatccg gtgcgtttat tctcaatgtg ctgggatggc
     7981 agccagaaaa tatcgccagc cagttgaaga aaatcgatga ccataccgtc caggtgcaat
     8041 ggagtgccga cgtcagcccg gcgctggcac tgaatattct ctccacacca atcgcctcaa
     8101 ttgttgacga gaagctggtt gcgccaaacg ccaaaaataa cgactttggt aatgaatggc
     8161 tgaagatgca ttccgctggc agcggcgcgt ttaaaatgcg tacttaccaa ccgcatcagg
     8221 ccatcgtgat ggaagccaac gccagctcac caaccggcgc cccgaaactg aaaaacgtca
     8281 tcatcaaaaa tgttcccgat ccggcaacgc gtcgcctgct gatccaacaa ggtgatgctg
     8341 atatggctcg tgatctggga gccgatcaaa tcgatgcatt acagggtaaa cctggcgtaa
     8401 aagtgatgag cattgcctcc gccgaacaga actacctggc cttcaatacc ggtaacaagg
     8461 ataacccgct gatgagtaat cctgcgttct gggaggccgc tcgctggttg gtcgattacg
     8521 acggcatcac caaaaatctc ttaaaaggac aatattttac ccatcaaagt ttcctgcccg
     8581 tgggctttcc tggagcactg gaagaaacac cgtttacctt taatccagcc aaagcaaaag
     8641 agatcctcgc taaagccgga atcaaagacc cgcacttcac gcttgatgtc gaaaataaac
     8701 cgccatttat taccatcgcg cagtcaattc aggccagctt tgctcagggc ggtgtaaaag
     8761 ttgatttgct gccagccgca gggagtcagg tctactctcg cgttcgcgcc caccagcatc
     8821 agggggccat tcgtatgtgg ttgcctgatt attttgacgc acattccaac gccagctctt
     8881 tcgcctataa cgatggcaaa tccagcaccg ttgccgggtt aaacggctgg aaaatcccgg
     8941 aactgaacaa agaaacgctg gcggccatcg ctgaacctga tagcactaaa cgtcttgatt
     9001 tgtataagaa aatgcaacaa gaactacaac gtagttcccc gtatgtcttt attgatcaag
     9061 gcaaaacgca aattgtcatg cgtgataatg tgcaggggta tcaacaaggg ttgaatgccg
     9121 atatggtctg gtttgatcag gtaaccaaat aaccctgtat gcaactcgcg tccagccatg
     9181 ttaccgggcg cggtaccagg gctggcctgt attgcaggcc agccgctgat ttatcactgt
     9241 atggaaagat catgtctgtc gttttttcca ctcgcgcgag atggcgaggc aagcgccctc
     9301 tgctcgcgct gttgcaaggg ctgtttaccg ttgctctgac gctactcggc ctgctgctga
     9361 ttacctttgc gctttcggct ctctcaccgg tcgatcgcgt actacaaatc gttggcgatc
     9421 acgccagcca gtccacctat gatcaggtac gccaccagtt aggattggat cagccgttag
     9481 ccgtccagtt ctggcattac ctgatgaatc tggcacacgg cgatctgggc atcgccagct
     9541 ccaccgggca accagtactc caggacttgc tatcagcatt cccggcgacc attgaactgg
     9601 caacgttggc gcttattgtc ggtgctgttg tcggcgtcat tgcgggtgta ctttgcgccc
     9661 gttatgtcgg ttctccgctg gatttcaccg tgcgaacatt aactctactg ggaaattccg
     9721 tccctgtttt ttggcttggt ctgctgatgc tggcgctgtt ctacgccaga ctgcaatgga
     9781 gtgccgggcc gggcaggctg gatgatattt atcaattcac catagaaccc cgcagcggat
     9841 ttgcgctgat tgatacgtgg ctttctggcg atcgcgaggc atttaccaat gcaatcaacc
     9901 atttgacact tccggtttta ttactggcgt acttctcgct cgccagtatt acgcgcctga
     9961 ctcgttccgc ctgtcttggc gaaatgaata aagaatatat tctgctggcg cgcgccaaag
    10021 gggccagcga gatgacaatt ttgctacgtc atgttttacc caacattcgc ggcaccttgc
    10081 tcactgttat cgccctcgcc tatacatcaa tgctggaagg cgcggtactg acagaaaccg
    10141 tcttttcatg gccaggtatt gggcgctacc tcactacagc gctcttcgcg ggtgatacca
    10201 cagcaatcat gggaggaacg ctgctgattg gcgtctgctt tgtgttgata aataacttaa
    10261 ccgacctgct ggtccggctg accgatccga ggttacgctg atgccgatgt ttctcttttt
    10321 acgccgttta cgccattcac ccgccgcgtt ttgcgggttg ataattatcc tgttattgat
    10381 gctaattgcg ctcttcgcgc cgtggctggc acctcacgat cctaactggc aggatgcggc
    10441 ggcgcggtta caagcaccga gtcgtcagca ctggctcggc actgacagtt acgggcgaga
    10501 cctactttca cgtttaatct acggcagccg tccggcactg gggctggtgg cattggtgac
    10561 aatcattaca ctcccggtcg gtctgctgat tggcatttta gccgggtact acggcggctg
    10621 gctggaacgc gtgctgatgc gttttactga cgtagtaatg tcaatgccgc gtttaattct
    10681 ggcttttgct tttgttgcca tgctggggcc ggggctggtt aacggcgcac tggcgctggc
    10741 attaaccacc tggcctgctt atgcgcgtca ggcacggagt gagatccaac gtctgcgaca
    10801 cagtgattat cttgccgccg cagaaatgat gggcatccgc ggcaggcgtc tgctggctgg
    10861 tcatatttta ccattgtgcc tgccatctgc cattgtccgt ctggcgctgg atttagcggg
    10921 tatcattctg gcggctgctg gcctgggttt tcttgggctt ggtgctcgcc cgccaatggc
    10981 ggaatgggga gccatgatcg ccgacggtat gcaggtcatt tttgatcaat ggtggattgc
    11041 cgccgctccc ggcgcaatta tcttaattgc cagtctggcc tttaatctgc tgggtgatgg
    11101 cttgcgcgat gttctggagc ctcaacatga ctgactcacg tcttagtatc aacaatctct
    11161 gcgtcgatta tccggagtcc cgggtcgtca acaatgtcag ttttacatta ggcaatgaac
    11221 gtctggcgct ggtcggtgag tcgggttcag gtaagtccat gaccgcccgc gccctgatgg
    11281 ggctggtgcg taaaccgggt gtagtaaccg cggacacact taacattctg gggcgtgatg
    11341 cactcaccct taacgcgcgt ggatggcaac agttgcgcgg taacgatatc gcgatggtat
    11401 tgcaagatcc gcgttacgcg cttaatccag taaagacagt aaaagcgcaa ctggaagagg
    11461 cgcttacgct ccatcaacgt ctgaaccgcc gcgagaaaga agagaaaata aacaccgcaa
    11521 ttcaggctgt cggcctcaat acgcgagtac tgcaaagcta tcctcgtgaa ctttctggcg
    11581 gcatggggca gcgagtgatg attgcaattg cgctcatcaa cgatccccgg gtattgattg
    11641 ccgatgagcc aacatcagca ctggatgtgc gcctgcgtaa tcagattctg gaactgctgg
    11701 ttacccagtg tgaacaacgt cagatggcaa tgttgctgat cagtcacgac ctgccattag
    11761 ttgcccaatt ttgccatcgg gtgctggtga tgtatcaggg aaataaggtg gatgaaatgc
    11821 acgctgctgc gctacctaca gcaacgcatc cttacacgcg cacactgtgg acttgccgcc
    11881 caaatgcaca aacttacggg cagatgctgc cgacgctgga caggacggca atgacgccgg
    11941 agaaatacca tgacgattgt tgaaattaat cacttacagg tgacttttgc tgagaaaacc
    12001 gcagtttctg ccgccagttt ttcggttaat gccggagaga catttagttt aattggcgaa
    12061 tcaggctgcg gaaaatccac gattttgcgc gtaattgctg gattacagcg cgactggcac
    12121 ggtcaggtgt cactgtttgg gcacaccatc aaaccaggaa tgcgttttca gggcgatctc
    12181 cgccgcaatg tgcaaatggt gtttcaggat ccctatgcct cgttgcatcc caatcatact
    12241 ctgtggcgaa cgctggctga accactcaaa attcgtggcg aaagagaaat agaaaaacgc
    12301 gtccagactg cactggaaca ggttggcttg cctttcgacg ccgctcgtcg ctacccacac
    12361 cagctttccg gtggtcaacg gcaacgtgtg gttattgctc gcgccctgtt gttacgacca
    12421 caacttcttc tgctggatga acccacctcg gcgttggata tgtcggtaca agcggaaatc
    12481 cttaatttgt tgaatcaact taagctggaa caccaaatga catatctgct ggtcagtcac
    12541 gatgccgatg tcatcgcgca tatgtctgat cgagcggcat tgatgtccga gggaaaaatt
    12601 cagcgatttt ttgatcgcga cgccatggaa aaggggaaac atcggatgga ttaaaaaacc
    12661 tgacattgca ttaattcatc ctgctttcag gtctttttac agttgttttt ttgtccaccc
    12721 gaacacttta tactgtctct cccctaccaa acttcattat caaaatgaaa aaaattatca
    12781 ctttgatgtt gtttttgaca ttctttgccc acgcgaacga ctccgagcct ggcagccagt
    12841 atttaaaggc agcagaggcc ggagaccgac gcgcacaata ttttcttgcc gacagctggt
    12901 ttagctccgg cgatttgagc aaagccgaat attgggcaca gaaagccgcc gacagcggtg
    12961 atgctgatgc ctgcgcgctg ttggcgcaga tcaaaatcac caatccggtc agtctggact
    13021 atccacaagc aaaagtgctt gccgagaaag cagcgcaagc gggcagtaaa gaaggtgaag
    13081 taacgctggc gcatattctg gtaaatacgc aagcgggtaa accggattat ccaaaggcga
    13141 tttcgctgtt agaaaacgcc tcggaagatc tggagaacga ctctgccgtc gatgcccaaa
    13201 tgctgcttgg tttgatttac gccaacggcg tgggcattaa ggccgacgat gacaaagcaa
    13261 cctggtattt caaacgcagc tctgcgattt cccgaaccgg ttattccgaa tactgggcgg
    13321 gaatgatgtt cttaaacggt gaagagggtt ttatcgagaa gaacaaacaa aaggcgctgc
    13381 actggttgaa cctgagctgt atggaagggt ttgataccgg gtgtgaagag tttgaaaaat
    13441 taaccaacgg ttaaggtagg cgcagcaaac gtcgcatcag gcgttgatgt cggatgcggc
    13501 gtaaacgcct tatccgacct acaaaaatca cgctacatca ggctattact cagtcaggca
    13561 tccacacatt gccgggtgga tatccccccg gcaatcttca attactgatc cgccgtttta
    13621 tcaaatttgc ccagcacttc acgctcataa gccagcgctt tcttacgg
//
LOCUS       DAWDRC010000029        13614 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2367_length_13614_cov_2.200679, whole genome shotgun sequence.
ACCESSION   DAWDRC010000029 DAWDRC010000000
VERSION     DAWDRC010000029.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13614)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13614)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2367_length_13614_cov_2.200679"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1445..1454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2949..2958
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3237..3246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5923..5932
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6936..6945
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7972..7981
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8837..8846
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11098..11107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12293..12302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2943 a         3305 c         3921 g         3355 t
ORIGIN      
        1 aacagcaaac cgcacgcgca tctggtgtgc ggttgcgccc gcccggagcc ataaacgaga
       61 acgcctttgc cagtgcctgt gtgcgttgcg gtcagtgtgt tcaggcttgc ccttacgaca
      121 ccttaaaact ggcgacgctg gcctctggtc tgtcggcggg cacgccatat tttgtcgcac
      181 gggatattcc ttgcgaaatg tgtgaggaca ttccgtgcgc caaagtgtgc ccgagcggtg
      241 cgctggatcg tgagatcgaa tcgatcgacg acgcacggat ggggctggcg gtactggtgg
      301 accaggaaaa ctgtctcaac tttcaggggc tgcgctgcga tgtttgttat cgcgaatgcc
      361 cgaaaattga tgaggccatc accctggagc tggagcgcaa cacgcgaacc ggtaagcacg
      421 cccgctttct gccgacggtt cacagcgacg cctgtaccgg ctgcggtaag tgcgaaaaag
      481 tgtgcgtgct ggaacaaccg gcaatcaagg tgttaccgct gtcactggcg aaaggggagt
      541 taggtcacca ttaccgcttc ggctggctgg aggggaacaa tggcaaatcg taaacgtgac
      601 gccgggcgcg aggcgctgga gaaaaaaggc tggtggcgca gtcaccgttg gttagtgtta
      661 cgtcgccttt gtcaattctt cgtgctgggg atgtttttga gcggtccgtg gtttggtgtg
      721 tggatcttgc acggcaacta cagcagtagc ctgttattcg acaccgttcc actgaccgat
      781 ccgctgatga cactgcaaag tcttgccagc ggtcatctgc ctgccacggt agcgctgact
      841 ggtgcggtga ttatcaccgt gctctacgcc ctggcaggaa agcgattatt ttgcagctgg
      901 gtctgcccgc tgaacccgat taccgactta gcaaattggt tacgcagacg atttgacctc
      961 aatcagtctg cgacgatccc tcgccacatt cgctacgtgc tgttggtggt cattctgctg
     1021 ggatcggcct taaccggcac gctcatctgg gaatggatca accccgtttc cctgatgggg
     1081 cgcagtctgg taatgggctt cggcagcggc gcgctgctta ttctcgcgct gtttttattt
     1141 gatctactgg tcgttgaaca cggctggtgc gggcacattt gtccggttgg tgcactgtat
     1201 ggtgtgctgg gtagcaaagg tgtgattacc gttgcggcca ccgatcggca gaaatgtaac
     1261 cgctgtatgg attgttttca tgtttgcccg gaaccgcatg tgctacgtgc cccggtgctg
     1321 gatgaacaaa gcccggtgca ggtcaccagc cgcgattgca tgacgtgcgg tcgctgcgtg
     1381 gatgtctgtt ctgaggatgt atttacaata actacacgat ggagttcggg agcgaaatca
     1441 tgaannnnnn nnnncggcga tgctggccct ggtggtaagc ggcgcggttt gggcggctaa
     1501 tggcgtcgat tttagccagt cgccggaagt atccgggacg caggaagggg ccattcggat
     1561 gccaaaagag caggatcgga tgccgctgaa ctatgtgaat cagccgccaa tgatcccgca
     1621 tagcgttgaa ggttatcagg taacgaccaa taccaaccgc tgcttgcagt gccacggtgt
     1681 cgaaagttat cgcaccactg gcgcgccgcg cattagtcct actcacttta tggacagcga
     1741 cggcaaagtg ggcgcggaag tggctccgcg tcgttatttc tgtctgcaat gtcacgtacc
     1801 gcaggccgat accgcgccaa tcgtggggaa tacctttacc ccatcaaaag gttacgggaa
     1861 ataagaggtt attatgggaa attctgaccg taagcttggt ctgattaagc gcctgtggaa
     1921 atggtggcgt acccccagcc gtctggcgct agggacgctg ctgttgatcg gttttgtcgg
     1981 cggcatcgtc ttctggggcg gctttaacac cgggatggaa aaagccaata ccgaagagtt
     2041 ctgcattagc tgccacgaaa tgcgtaacac ggtgtatcag gaatacatgg attccgtgca
     2101 ctacaacaac cgtagcggcg tccgtgcgac ctgtccggat tgtcacgttc cgcacgagtt
     2161 tgtgccaaag atgatacgca agctcaaagc aagtaaagag ctgtatggta aaatttttgg
     2221 cgttattgac acgccgcaga aatttgaagc tcatcgcctg acgatggcac agaatgagtg
     2281 gcggcgcatg aaggacaaca actcgcagga gtgccgtaac tgtcacaact tcgagtatat
     2341 ggatacaacc gcccagaaat cggttgccgc gaagatgcat gaccaggcgg tgaaagatgg
     2401 gcaaacctgt attgattgcc ataaagggat agcgcacaag ctgcccgaca tgcgtgaagt
     2461 cgagccaggt ttttaacagg gttattgcgt gggtatgctt gaagccagag agttactttg
     2521 cgagcgggat gaacgaacct tatttagtgg tttatcattt acgctgaacg caggagagtg
     2581 ggtacaaatc accggtagca acggcgcggg gaagacaacg cttctccgtt tgctgacggg
     2641 gttgtctcgc cctgatgcag gcgaggttct ctggcaaggg cagcccttgc atcaggtacg
     2701 cgacagctac catcaaaacc tgttatggat aggccatcag ccggggatca aaacccggct
     2761 gacggcgtta gaaaatctgc acttttatca tcgcgatggc gataccgcac aatgtctgga
     2821 agccctggcg caggccgggc ttgccggatt cgaagatatt ccggtaaatc aactttctgc
     2881 cgggcaacaa cgccgcgtcg ctttagcgcg tctgtggttg acccgtgcca cgttatggat
     2941 actcgacgnn nnnnnnnnct tttaccgcga ttgacgttaa cggcgtcgat cgtctgaccc
     3001 agcgtatggc gcagcatacg gagcaggggg ggattgtgat tctgactacc caccagccgc
     3061 tcaacgttgc tgaaagtaaa attcgccgca tttcactgac gcaaacgggg gccgcatgat
     3121 gttctggcgc attttccgtc ttgagctgcg cgtagcgttt cgccatagcg ccgaaatcgc
     3181 caacccgctg tggttcttcc tgattgtaat tacccttttt ccgctcagta tcggtcnnnn
     3241 nnnnnncaac tgctggcgcg tattgcgccg ggcattatct gggttgccgc gctgctttca
     3301 tccttgctgg cactcgagcg actgttccgt gacgatttgc aggacggcag tcttgaacaa
     3361 ttgatgttgt tgccgttacc cttgcccgcc gctgtgctgg cgaaggtgat ggcgcactgg
     3421 atggtaaccg gtctgccgtt actcatcctt tcgcccctgg tagcaatgct actgggaatg
     3481 gatgtttatg gctggcaagt gatggcgtta acgctactgc tgggaacccc tacgcttggc
     3541 tttcttggtg cgccgggcgt ggcgctgaca gtgggactta agcgcggtgg tgtgctgctc
     3601 agcatactgg tgttaccgct gactatccca ttactcatct ttgccaccgc cgcgatggac
     3661 gcggcttcta tgcatttgcc cgttgatggg tatctggcaa ttttaggcgc gttgctggca
     3721 ggcaccgcga cattaagtcc ttttgcgacg gcggcagcgt tacgaatcag cattcaataa
     3781 cgctggatta ctgatttttg ttcgagtctg gtatcgaaac tatgtggaaa acactgcatc
     3841 aactggcgat cccaccacgg ctgtatcaaa tctgtggctg gtttataccg tggctggcaa
     3901 ttgccagtgt ggtcgtgctt accgtcggct ggatctgggg gttcggcttt gctccggctg
     3961 attatcagca gggaaatagc taccgcatta tctacctgca tgtgcctgcg gcgatctggt
     4021 cgatgggcat ttatgcatca atggcggtgg cagcgtttat tggcctggtc tggcagatga
     4081 aaatggccaa cctggcggtg gcggcgatgg cccccattgg tgccgtgttt acctttattg
     4141 ccctggttac cggctctgca tggggaaaac cgatgtgggg cacctggtgg gtatgggatg
     4201 cacgtctgac ttctgaactg gtgctgctgt ttctgtatgt tggtgtcatt gccctgtggc
     4261 acgccttcga cgaccgtcgt ctggctggcc gtgcggcagg tatcctggtg ctgattggcg
     4321 tggtgaatct gccgattatt cattactccg ttgagtggtg gaacaccctg catcagggat
     4381 caacgcggat gcagcaaagt atcgatccgg cgatgcgttc gccgctgcgc tggtcgattt
     4441 tgggcttcct gctcctgtct gccacgctga cgctgatgcg gatgcgtaat ttgattttgc
     4501 tgatggaaaa acgccgtccg tgggtgagtg aactgatact gaaaagaggc cgtaaatgac
     4561 ccctgcattt gcttcctgga atgaattttt cgcaatgggc ggttacgcct tttttgtctg
     4621 gttggcggtg gtgatgaccg ttattccgct ggtggttttg gtcgtgcact cggtgatgca
     4681 acatcgcgca attctgcgtg gcgtggcgca acagcgggcg cgtgaggcgc gtttacgtgc
     4741 tgcgcaacag caggaggctg catgaatatt cgccgtaaaa accgcttgtg gattgcctgt
     4801 gccgtgctgg cggggctggc gctgactatc ggtctggtgc tatatgcgct gcgctcgaat
     4861 atcgatctct tttatacgcc gggggagatt ctctacggca agcgtgaaac tcagcagatg
     4921 ccggaagtcg gtcagcgtct gcgcgttggc gggatggtga tgccggggag tgtgcagcgc
     4981 gatcccaatt cgctaaaagt aactttcacc atttacgacg ctgaaggttc agtagatgtc
     5041 tcttacgaag gcattttgcc ggatctgttc cgtgaagggc agggcgttgt ggtgcagggc
     5101 gaactggaaa aaggcaatca tatcctcgcg aaagaagtgc tggcgaaaca cgatgaaaac
     5161 tacacgccgc cagaagttga gaaagcgatg gaagctaacc accgtcgccc ggcgagtgtt
     5221 tataaggacc cagcatcatg atgccagaaa ttggtaacgg gctgctgtgt ctggcgctag
     5281 gaattgcgct gctgctgtcc gtgtatccgc tatggggcgt ggcgcgcggg gacgcgcgca
     5341 tgatggcgtc ttcccgcttg tttgcctggc tgctgtttat gtctgtggct ggcgcatttc
     5401 tggtgctggt caatgctttc gtggtcaacg actttaccgt cacctatgtt gccagcaact
     5461 ccaataccca gcttccggtg tggtatcgcg tggcggctac ctggggcgcg catgaaggct
     5521 cgctcctgct gtgggtgctg ctgatgagcg gctggacctt tgcggtagcg atttttagtc
     5581 agcgtattcc gctggatatt gtggcccgcg tactggcgat aatggggatg gtcagcgtcg
     5641 gctttttgct gttcattctc tttacctcta acccgttctc acgcacgttg ccgaacttcc
     5701 cgattgaagg tcgcgatctt aacccgctgt tacaggatcc ggggctgatc ttccatccgc
     5761 ctctgcttta tatggggtac gtcggcttct cggtggcgtt tgcttttgcc attgcatctt
     5821 tgttgagtgg gcgtctggac agcacttatg cgcgttttac tcgtccgtgg acgctggctg
     5881 cgtggatctt cctgacgctc ggcattgtgc tcggttccgc atnnnnnnnn nnattacgaa
     5941 ctcggctggg gcggctggtg gttctgggat ccggtagaaa acgcctcgtt tatgccgtgg
     6001 ctggtgggga ctgcgctgat gcactcactg gcggtcactg aacaacgcgc cagcttcaaa
     6061 gcgtggacat tactgctggc aatcagtgcc ttctcgttgt gtctactggg gactttcctg
     6121 gtgcgttccg gcgtgctggt atcggtacac gcgtttgcgt ctgatccggc gcgcggtatg
     6181 tttatcctcg cctttatggt gctggtgatt ggcggttcgc tgctgctgtt tgccgcgcgt
     6241 ggacacaaag ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc tttgctgtta
     6301 gcgaacaacg ttttgctggt cgccgcgatg ctggtggtgt tgctggggac gttgctgccg
     6361 ctggtgcaca agcaactggg actgggcagt atttcgattg gcgaaccgtt cttcaacacc
     6421 atgtttacct ggctgatggt gccgtttgcg ctactgcttg gtgtcggccc tctggtgcgc
     6481 tggggacgcg atcgcccacg taaaatccgc aatttgttga ttatcgcctt catctctacg
     6541 ctggtgctgt cgctgctgtt gccgtggctg ttcgaaagca aagttgtggc gatgacggtg
     6601 ctcggcctgg caatggccgg cgggattgcg gtgctggcaa ttgcggaagc tgcgctgcgt
     6661 atttcacgcg gcacgaaaac caccttcagt tattggggga tggtggcggc tcacctgggg
     6721 ctggcagtga caattgttgg cattgctttt agtcagaact atagcgttga gcgtgatgtg
     6781 cgcatgaagt ccggcgatag cgtcgatatt catgaatatc gcttcacctt ccgtgatgtt
     6841 aaagaagtga ctggtccaaa ctggcgtggc ggtgtggcga ctatcggcgt aacgcgagat
     6901 ggcaagccgg aaacagtgct gtatgcagaa aaacgnnnnn nnnnngggtc gatgatgacc
     6961 gaagcggcga ttgacggtgg catcacgcgt gacctgtacg cggcgctcgg tgaagagctg
     7021 gaaaacggcg cgtgggccgt gcgtctttac tacaaaccat ttgttcgctg gatttgggcg
     7081 ggcgggctga tgatggcgtt gggcggactg ctgtgtctgt ttgatcctcg ctatcgtaag
     7141 cgcgtgaacc cgcagaaaac tgcgccggag gctgcatgaa gcgcaaggta ttgttaattc
     7201 cgttgattat cttcctggcg attgccgcgg cgctgctgtg gcagctggcg cgtaatgccg
     7261 aaggggatga tccgaccaat ctggaatcgg cgctcattgg caagccagtg ccgaagtttc
     7321 gcctcgaatc actggacaat ccggggcagt tttatcaggc ggatgtgctg actcagggca
     7381 aaccggtgct gcttaacgtc tgggcgacct ggtgtccgac ctgccgtgcg gaacatcaat
     7441 atctgaatca gctttctgcg cagggcatcc gcgtggtcgg catgaactat aaagacgatc
     7501 gccagaaggc aatcagctgg ctgaaagagc tgggcaatcc ttacgcgcta agcctgtttg
     7561 atggcgacgg catgttaggg ctggatctcg gtgtctatgg cgcgccagaa acgttcctta
     7621 ttgacggcaa cggcatcatt cgctatcgcc atgcgggcga tctcaatcct cgcgtctggg
     7681 aagaagagat caagccactg tgggagaaat acagtaagga ggccgcacaa tgaggttttt
     7741 attgggcgtg ctgatgctga tgatctccgg ctcagcgctg gcgaccatcg acgtgttgca
     7801 gtttaaagat gaagcacagg aacaacagtt ccgtcagctc actgaagaac tgcgctgccc
     7861 gaaatgccag aacaacagca ttgccgattc caactcgatg attgccaccg acctgcgcca
     7921 gaaagtgtat gaactgatgc aggaaggcaa aagtaagaaa gagattgtcg annnnnnnnn
     7981 nattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt aacgccgctg
     8041 accgtgctgc tgtgggtgct gccagtagtg gctattggca ttggcggttg ggtcatatac
     8101 gcccgttcgc ggcgtcgggt acgcgtggtg ctggaagcgt ttccagaaca aagcgtgccg
     8161 gaaggtaagc gtgccggata tgttgtttat ctgccgggta ttgtggtggc gttaattgtg
     8221 gctggcgtca gctactacca gactggcaat tatcagcagg tgaaaatctg gcagcaggcc
     8281 acggcacagg ctccggcgtt actggacagg gcgctggatc cgaaagccga tccgctcaac
     8341 gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa aaatccggga
     8401 gatatagaag gctggattat gttgggccgc gttggcatgg cgctgggtaa cgccagtatc
     8461 gccaccgatg catacgccac tgcgtatcgc ctcgatccga aaaacagcga tgctgcactg
     8521 ggatacgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct cggcggcgaa
     8581 ctgctgcgcc agttggtgag aacggaccat agcaatatcc gtgtgttaag catgtatgca
     8641 tttaatgcct ttgagcagca acgatttggc gaagccgttg ccgcgtggga gatgatgttg
     8701 aaactcttac ctgccaacga tactcgccgt gcggtgatcg aacgtagtat cgcgcaggcg
     8761 atgcaacatt tgtcgccgca ggagagtaaa taaaaagtgg cccgatggtg atgccatcgg
     8821 gctattttta ttgtgcnnnn nnnnnnccac cattctgttg caggaacaga atggtggccg
     8881 ccacgcgtga gcggacgttg agtttacgca gcagattgcg aatatggact tttactgtct
     8941 gctcggaaat attcaacacc gaggcaatct gtttatttga cagcccctgt gccagctcgt
     9001 gcagaacatc cagctcgcgt tctgtcagca cgctgaaggg gtcttcttcc gcgccaaaca
     9061 tttcacgttc acgtaagtac tgattgacgc gttcgctaaa gactttgctg cctttcgctc
     9121 cggcacgaat tgcttccagt aacacttccg ggtcgctgtc tttcaacaga taaccgtctg
     9181 cgcctgcgtc tatcagtgca aagacatcgc tggaggcatc tgatacggtc aggataataa
     9241 tttgcgcggt aacgccatcc ctgcgcaagg cattgagggt atccaggcca ctcatgcctt
     9301 tcatattgag atccagcaag atcacgtcga tatccagtct attcgccaga tcgatcgcgc
     9361 ttgcgccgtc gcccgcttcg gcgaccactt caaagccaga atcaagctcc agtaactgac
     9421 gaacaccgcg tcgcataagt ggatgatcat ccacaatcat cacctgaaaa ggcgttgctt
     9481 caggcatagt agtctcctga ggttttatta gaatcattat gtttgagcgt agcgttggta
     9541 acgctaccag gcgcgtgaat gttaccccat tttatgtatg gacatgtagt ggaaactaca
     9601 actttgtcgt tcgctttggc atcatcttca ttataaagac ggaattttgc atcagcttct
     9661 catcaggaaa aacaactaaa aaatcaatca caatacttgt gcatttcttt aaacagtttt
     9721 taaaaactga ctccgggtat ggagctatgg gtattttctg tccccaatgc ttttaacagc
     9781 aattaatttc ataggatgaa agcttaatgc accaatctgg ttctgtttct ctttgtcgtt
     9841 ccgcaatatc tgttctggtg gctacagcgt tatattcgcc catagcattg gcatcaactg
     9901 ttgagtatgg tgagacagtt gatggtgttg tcctggaaaa agatatccag ctggtttatg
     9961 gggccgccaa taatacgaaa atcaatcctg gcggagaaca gcatatataa gaatttggtg
    10021 taagtagtaa tactgaaatt aacgggggat atcagtacgt tgaaataaat ggcaccgcag
    10081 aatacgcagt attaaatgat ggttatcaaa ttgttcaaat gggtggcgcg gtaaatcaga
    10141 ctacgatcaa taatggagtg ctacaggttt atggcgcggc gactgatccc acgattaaag
    10201 gagggcgcgg tgatgcggca tttacgttgg gcaatgccgg aggcgttgtt gatatcagta
    10261 cgtatgaata taccttgctg gataatggca accatagctg gagtctcgca gagaatcgcg
    10321 tacaaatgcc cccttcaacc acggatgtgc tgaatatggc ggcagcacaa ccgctggtat
    10381 ttgatgcaga actggacacc gtgcgtgagc gtcttggtag cgtaaagggc ggcgatgtgg
    10441 agttcggcaa ttaacagccg caacagcgtg aacactgatg cgggagctgg ctttgagcaa
    10501 acattgacgg ggctaacgct tggtatcgac agccgtttct cctgtgaaga aagcagcaca
    10561 actcaggcgg gtgtagtctg gacgttctga taacagaaaa taaacaggcc gtgatgtgtc
    10621 gcggtctgtt tatcgaatta aatgcagata taaaaaaacc aaccgcaata ggttggtttt
    10681 cttgggattt ttggtcggca cgagaggatt tgaacctccg acccccgaca ccccatgacg
    10741 gtgcgctacc aggctgcgct acgtgccgac tcgtggctgc taatactacc gttttccaca
    10801 ccgattgcaa gtaagatatt ttgctaactg atttataatt aatcagttag cgataaaacg
    10861 cttctcgtct gtcagtactt gcaataacaa acttaactgt ggtttttcat ctttcactct
    10921 ttcaccacgt aagttgtaag tgcggtattt accgttattg ttcagcacca gcgtcttttt
    10981 cggggtggta attgccagcg tatcgttatc cgcggcggta acccagtaat ggcggcgttg
    11041 agggttgaac aaatcctgac cttgcgaata ttcgctggca ggtgtgctga catgtagnnn
    11101 nnnnnnnagg cgttgcatca gcgtcgtcat cagatcggta tgatcagtca gcgcattaat
    11161 acgctgcgcc ggcgtgcctg gccagtgaat cactaatggc acctgcagat gaccgtggga
    11221 ccagtcaaag gtttcttcct cttcgctcag cggaataccc cgcccggcag tgataatcac
    11281 caccgtattg tccagtttgc cagaatcacg cagtgcattg agcacgcggt tgatctggtc
    11341 atcgacattg cctgccgccc ggctatattt ccgtgcaaat gcctgctgat tgctgtcgtc
    11401 aatgtttgtg ccattgaaag agacccacga gaaccagcgg ttatcttctt gtgcgtagcg
    11461 gcccagccag ttgatccact gcgtggcggt ctgttcgtcg gattgggtgc gtacactcgg
    11521 catcgagaaa tctgacaaca atgcctgacg atacagcggg ctggtaaagc catctgatga
    11581 gaataacccc agctgatagc cttgctgatt aagcgcagta attaacgccg caggcgtacg
    11641 agtcgacaga atgccgtcca tatagctcgg cgagatgcca tagaacaggc caaagatgcc
    11701 gttgtctgta gtgttgccgg agctcatatg gcgcgtgaac gaaatatttt gctcagcaaa
    11761 acctgccagc gcaggcatct gcttctcgaa gcgtgagtag ttcaggccat cgacagtaat
    11821 caacagcaca ttctgcccgg tgcccatatc gcgatagcgc agttcgctta acggatactg
    11881 aacggaaacg gcgtctggat tgccttgctc aataagacgg cgttgatact cctgcgcatc
    11941 aagcaggcca tgcttctcaa gaaaacgtcg tgccgtcatc gggtacgaaa gcggcaggtt
    12001 agcgcgctgc atggtgatcg ggcgatagaa gttggcatcg gcccagatat aaaccacatg
    12061 cgaggcgata aaggtgataa ataagaatgc ggccagcggg cgcgcgaagc gccgacgacg
    12121 cgtcaggctg cgcagctttt gccagctcca cgtcgcaaac accagttcaa gcaataaaat
    12181 aaccggcaca ctgatgaaca ttagctgcca gtcgcgcgcc atctcatttt cgtctgggtt
    12241 gataaccagc tgccagacga tgggattaag atggagatgg aaacgagtaa agnnnnnnnn
    12301 nnaagtaata gcgtcattcc cgccgttgcc agaatgacgg acaaaaacct catcagcctc
    12361 tgggagccga cgataaaggt tagcgggaag aggatcagca agtaggtggc gaacaccagg
    12421 aagctgaaat gaccgataat gcttacgtag gaataaatgc gaccagcaag cgttgtcggc
    12481 cagtcggcga taaacaggta acggctgcca atgacgagcg aaagcagaat attgaacagt
    12541 gcaaaccagt gcccccaact gaccatctgg gagacttttt cacggtagcg ctgacgatga
    12601 gttaccataa cctgttatct gtttccctta gtgcgctttg tcttcgttga tagaggactg
    12661 taaggcgcgg gcaaaagagt tggcaattgc ctggcgttgg gccggggcaa tgctggtgtt
    12721 gataaggtta gtgaccatgt tgcctaacac catcagggaa aggtcggtcg gagccttatg
    12781 tttttccagt acgttgagca gctctgcgag caattgttca acctgttcat cactgtagcg
    12841 ggaaatttgt ggcataaatc gaaatcagtt agttgatgaa agggcaacat attaccgtag
    12901 caacggtttt ttttctgctt ttttatgcgt taaatcattc tcgttacgcc gccttctgta
    12961 aaatgaccgc catcttttat tgcgtcgctt aaccggcggt ggttgaatac cgcccggtct
    13021 taaaggagag tttatcatga gtctggatat caaccagatt gctctgcacc agcttatcaa
    13081 gcgtgatgag cagaaccttg agctggtgtt gcgcgattca ttgctggaac cgacggaaac
    13141 cgtcgttgag atggtggcag aactgcatcg ggtctatagc gccaaaaata aagcttacgg
    13201 cttgtttagc gaagagagtg aactggcgca gacgctgcgc ttacagcgtc agggcgaaga
    13261 ggaccttctg gcatttagcc gcgcggcaac cggtcgcctg cgtgacgaat tagcgaaata
    13321 tccttttgct gacggtggat tcgtgctgtt ttgccactat cgctatctgg cggtggagta
    13381 tctgctggtg gcagttctga gcaacctgag cagtatgcgt gttaacgaaa atctcgacat
    13441 caacccaacc cattatcttg atatcaatca tgcggatatt gttgcgcgta tcgatttaac
    13501 cgagtgggaa accaatccag agtcgacccg ctatctcact ttcctgaaag ggcgagtagg
    13561 gcgcaaagtg gcggatttct ttatggattt cctcggtgcc agcgaagggt taaa
//
LOCUS       DAWDRC010000030        13392 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2408_length_13392_cov_3.149434, whole genome shotgun sequence.
ACCESSION   DAWDRC010000030 DAWDRC010000000
VERSION     DAWDRC010000030.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13392)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13392)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2408_length_13392_cov_3.149434"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    11645..11654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12040..12049
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3597 a         3063 c         3148 g         3564 t
ORIGIN      
        1 tgggggtgga tgctggatgc tatgccacgc tggtgcattt tccggtcagg ccggcggtgt
       61 ggctggaacg cgatgatgat gctggtttac gggatatgct gaagcgtgct gactatctcg
      121 cgaaagagta caccaaagtc aacggtaccg gtgagcgcaa ttttggctgt agccggagtt
      181 gaacacgaca ggcgcaccaa tacccggcaa accaaaagta tgggttgttg tctagcggca
      241 acgatgtatc cactcgccca ctgactgttt tttacgtcag tgttccattg tgtgacaacc
      301 acagtgctgt aacagcaacc agcctgcttt acccaaactc cagtccagat tttaactgaa
      361 ccgccattca caccctgacc acgctgcccg taccgggacg gctgttgcct gcgtgtcgtc
      421 tggcggctga ggaatatgac tatgtacgca aaatcctttc tcgcacttga tggcaacgga
      481 cgtctgacgg gcgcccgtac tgcacaggcc gcaccttatg ctcactacac ctgccacttg
      541 tgtggcagtg cactcagata ccacccgcaa tacgacactg agcgtccctg gtttgaacac
      601 actgacgacg ggctgacagc gcacggtcag cagtgtcctt atgtcagacc ggaacgcagg
      661 gaggtacgct tgattaagcg tctgcagcaa ttcgtaccgg atgccttacc cgtggtgcgt
      721 aaagccacct ggcactgcag acaatgtcac cacgattatt atggggagcg gtactgcaca
      781 cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat atgcgaattc
      841 tgaactgtta tatggcgaat gacagcaagg ggcattttgt tacggcgaaa gaagcagcga
      901 agcacaaccg ccgggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta acacttcagc
      961 ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc gctgagaaaa
     1021 tcctgctgcg atgcacctgg cttgacccgg cagagaaaga ggcccgtcgt ttgcatctgc
     1081 agggtatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt gtgatgtgtg
     1141 acgaagatta tgaaggagaa aagtgctgcc cgcgttgtgg taccggggta tacagcaggg
     1201 cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg ttacagaggc
     1261 tgtaatggtt gcctctggcg cagtttgcgg tgatgccgtt cccgggaatg gattgccagt
     1321 gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt tatgccaaca
     1381 gcccccctgt gactgacagg ctgcctcgtc attccattcg tatctccagt aacaggattt
     1441 tattgtgccg gttcaggcgt gcgtttcccg gcgcgtctgc accggcttaa ccaaattcaa
     1501 cagggataaa atagtgtaaa taaccgaagt attacttgca gaatcggggg aagcatccgg
     1561 ggttttattc aacaggctgt agatcactga ctgcgtctgt tggccgtcca cttcacgcgt
     1621 cattcgacca gagtaaatat tgatgattac tagattcgtg tattttatca ggcattttac
     1681 ttgcgaataa atccccttgt tatccagcga aaccacacag gtagctatta ccatatgtcg
     1741 taatgcacta gttacaggaa aaacatgcag gttccgcaat acgttgtgtg ttgctgctta
     1801 gtcaggatct tttcttccat gcgagatacg acaacacttt tgaggaagga tgttcgtaac
     1861 tggtagagtt actacaacag gcatgggagg ctccggctct gacagacatt gtactgtgga
     1921 atgttgttac aggttggggc ggcaaccgtg ccaggagacc atgagccgtt cacggcatta
     1981 aaggccacac ttcagtcgtt tacattccct gtggtggcag gcatcttccg ttaaggcgtc
     2041 agagcgttac caacgttatg ttgtcatggc tcacctttca tagtcgatgc ttatctggtg
     2101 actgaaacta gccgcagata aagagtgtga agttgatacc atggagtgga gtgacgtgca
     2161 ggcaggagat tcgctctatg cagggttgtg agatgaattc aaaccggctg tcagcgccag
     2221 tgatatttga agattcgtcc ggttgttatc cagtgtgcat taaaaaccca gacattatgg
     2281 acaatacaac ggcttccata acagggagga gcctgtttat tatccgggcg taatgactca
     2341 cccttgtggg ctttttcccg tcgcgctttt aacgttgaag gcattttctg cctgccgatt
     2401 caggacagcg acaaaagcca tatcagacta ttggtcaatg atgaccagcg tctggagtag
     2461 atgataagcc aaattgataa gctggaatat gtgatgaaag tgcaacgtaa tcagtccaat
     2521 ccgacgatgt ttaacaagag tacggtgttt tttcagtaag tcattcgtct tctgaatcag
     2581 gtgtaatgac ctttacagaa gcattccggc agtgcgtaac ataatctgcc ggtaacggac
     2641 gatcgctaat cagaacatca aatgcagaga gcggtgcgag gctggcagag gctacttcat
     2701 cgaacaaggc gtggcgggcg agcaaaattt tccttagtcc acgggccatc gctttacgct
     2761 tagtggcaag atcttcggga ttaaaccagc tgacgccaaa gtgatcatgt acaccgctgg
     2821 cggaaataaa tatttttcgc ggattgagag agtctaatgg cgaagagtta ctggcatcgt
     2881 aaaaagcatc acttctggca cgatacgtac caccacaaag tattgctgtc acattaggtt
     2941 tttcattcaa cgcaacaaag acacgatgtg agtaacagat gccagtgaag gtgattgcat
     3001 ccgggatcat gcttataacg agcggtatct cctggccatt atcaaagaag atcagatcat
     3061 tttcattaac cattccggcg gccagaattg caataggtaa gtcatcacga tgatttctcg
     3121 gaacgtcctg gattactggc atggatggtg cgggtttatg caccattaca atatagccac
     3181 ccagtagggt cagtggcaga ggttcatctt cctgatggag atcgcgacga atagtcatta
     3241 cagaaacttc cagcattcgt gccgcgtctt tcaaatggat tctgtcggtt ttcttaagta
     3301 tttcaatcaa acgtcggata cgctcttttt gcttcgtttc catcaactct ctccataaaa
     3361 atgttataaa aataacatta tggcattagt aaacattgtt ttagttacca tactatacga
     3421 ccgacccttt gaaatgaaaa gggtatagac tgctgaattt tcggtattaa tccggttagg
     3481 ttgcttatga ttagatagtg ttggcctgag tgataagact ggaagtctat taaataaaca
     3541 tcgggttgat gtgctaatcg cgatctcagt cacaaatgat gcaaaagtaa catgctaatg
     3601 ttatttaaat accattgtta tgcgaaagtt tataagaggt agtgaaatgg acattgcagt
     3661 tattggttcc aatatggtgg atctcattac ttacacgaat cagatgccta aagaaggaga
     3721 aactctggaa gcacctgcct tcaaaatcgg atgtggtggg aaaggtgcta atcaagcggt
     3781 ggcggcggct aaattgaatt caaaagtcct gatgctgact aaagtaggtg atgatatttt
     3841 tgcggataat actattcgta atctcgaatc atggggaatt aataccactt atgttgagaa
     3901 ggtcccctgt accagcagtg gtgttgcacc gatctttgtt aatgccaact cgagcaatag
     3961 tatattaata attaagggag cgaataaatt tctttccccg gaagatatcg atcgagctgc
     4021 agaagatctg aaaaaatgca aattgatcgt tttacaactg gaagttcaac tggaaacagt
     4081 ttaccacgcg attgagtttg gtaagaaaaa tggtattgaa gtgttattaa atccggctcc
     4141 ggcattgcgt gagttagata tgtcttatgc ctgcaaatgt gatttcttta tcccgaatga
     4201 aacagaactg gaaatattaa ccggtatgtc ggttgatact tatgatcata ttcgtttggc
     4261 cgcacgttcg ttagttgata aagggctgaa caatattatt gttaccatga gcgaaaaagg
     4321 cgcattatgg atgacccgtg atcaggaagt ccatgttcct gcattcaaag taaatgctgt
     4381 tgatactagt ggcgctggtg acgcatttat tggttgcttt tcccattatt atgttcaaag
     4441 cggtgatgtt gaagccgctt tgaaaaaagc cgctctcttt gccgctttca gcgtcacagg
     4501 gaaaggtacg caatcatctt atccgagtat tgaacaattt aatgaatttt taaccttaaa
     4561 cgaataatca tcctgcacag ttaaaggtag cactatgaac gataaaaaca tcattcagat
     4621 gcctgatggg tatctgaata agactccctt gttccagttt attttgttat cgtgtttatt
     4681 cccgctatgg ggatgcgcag ctgcattaaa tgacatatta atcactcaat tcaaaagtgt
     4741 attttcgcta agtaactttg cttcagcatt ggtgcagagc gcattttatg gaggttattt
     4801 tttaattgca ataccagcat cccttgtgat taaaaagacc agttataagg tggcaatttt
     4861 aattggcctg acgctgtata ttggtggttg tacacttttt ttccctgcct cacatatggc
     4921 aacctacact atgttccttg cagctatttt tgcgattgcg attggcctga gtttccttga
     4981 aactgcagcg aacacataca gttcaatgat cggtccaaaa gcctatgcca cattgcggct
     5041 caatatcagc cagacctttt atccgattgg tgctgcgtct ggtattttat tgggcaaata
     5101 tctggttttc tccgaggggg aaagccttga aaagcagatg tcaggaatga acgccgagca
     5161 aattcacaac tttaaggtgc tgatgttaga gaacaccctt gaaccgtata agtatatgat
     5221 tatgattctg gttgtggtca tggtgttgtt ccttttaacc cgcttcccaa cctgtaaagt
     5281 ggcacagaca agtcaccata aacgcccgtc agcaatggat acgctgcgct atctggctag
     5341 aaatccgcgc tttcgtcgcg gtattgtggc gcagttcctt tatgtgggta tgcaggtcgc
     5401 agtatggtca tttaccattc gtctggcgct ggagttgggc gatatcaacg aacgtgatgc
     5461 ttcaaacttc atggtctata gctttgcctg cttctttatt ggcaagttta tagccaacat
     5521 ccttatgacg cgttttaatc cggaaaaagt attgattctc tattccgtta taggggcgct
     5581 gttcctggca tatgttgcac tggctccaag tttcagcgcg gtctatgtcg ccgtcctggt
     5641 aagtgttttg tttggccctt gctgggcgac gatctatgca ggaacactgg atacagttga
     5701 taacgaacat acagaaatgg caggagcagt cattgttatg gctattgtcg gtgccgcggt
     5761 ggttccagca attcagggat atattgcaga tatgttccac tcactacagc tttcattcct
     5821 ggtttctatg ctctgctttg tctatgtcgg agtctacttc tggcgtgaaa gcaaagtacg
     5881 taccgcactg gctgaagtca cagcgtcctg aggagaaccg ttatgagtac gcgtattaat
     5941 ttgtggcggg cgctttttgg cgagaaacct cgtatcttgc tggaaaatag tgattttact
     6001 gtcacgtctt ttcgctatga cagcggtgtc gaggggctta aaattgcaaa cagtcgtgga
     6061 catttgatta ttcttccctg gatggggcag atgatttggg atgcgcaatt tgatggacac
     6121 ggcctcacca tgtgcaatat gtttcgccag cctaaaccgg cgacagaggt gatagagacc
     6181 tatggctgtt ttgcttttca ttcaggtttg ctggctaatg gctgcccatc tgcggaagat
     6241 actcatctgc tacacggtga aatggcctgt gccgcgatgg atgaagcatg gctggaatta
     6301 gacggagata tgttacgcct taacgggcgt tacgagtatg taatgggttt cggtcatcat
     6361 tatctggctc aaccaacagt ggtccttcac aaatcctcta cactgttcga tattaagatg
     6421 gccgtaacca atctggcttc tgtagatatg ccactacaat atatgtgcca catgaattat
     6481 gcatatatac caaatgctac attcagccag aatatcccgg acgaaatatt aagattgcgt
     6541 gaatcagtgc cttcgcatgt taatcccacc gctcagtggc tggcgtttaa tcaacggatt
     6601 atgcagggag aagcctctct gtcgacgctg agtcaaccgg aattttatga tccagaaatt
     6661 gtcttctttg cagacaaact ggacgcttat acagatcagc cagagtttcg catgatctct
     6721 cccgatggca ctacgtttgt tactcgtttc tacagtgctg aactaaatta tgtgacacgc
     6781 tggatccttt ataacggaga gcaacaggta gccgcctttg cattacctgc aacctgtcgg
     6841 cctgaggggt atcttgccgc gcaacgtaat ggcacgctga ttcaggttgc accgcagcaa
     6901 acgcgcactt ttacagtcac cacaggaatt gaataaccac tcaaggcctg aacagcggcg
     6961 cgcttatcgt taaggtaagc gcgctttttt acccgccagg acaagactat gatagcgttg
     7021 cccttataga cgatcacatt atcgtccact ccaaatttgc gcaactactg tagagtggtg
     7081 tactccacag cttctttgag gatctcaact tccattgttt tcttaccgag caagcactgc
     7141 aactctttta tttatttgta gtggtcaaca aaaactggtc acaatttcag tttttttcca
     7201 gtatctattt tccgattcgt tcggcggtaa tccaccgtta tattcatgag gccggagtgc
     7261 actataaaag tgtatgcgta aaccgactgc tgtatgtagc cattaagcca gtattggtaa
     7321 cataggtacc caccttttca actagtggac acctgttatg gaacaaaaaa cattatcagc
     7381 tgaaccccgc aggtcatttt caaatgagtt taaacttcaa atggttaaac tggcttcaca
     7441 accaaggggt tctgttgccc gtattgcccg ggagcatgat atcaatgata atcttctgtt
     7501 caaatggctc aggctctcgc tgatcaaagg gcgtatatca cggcgtcttc cgttaacgaa
     7561 ctcttctggc attggcgttg aattattacc tgtggagatg acgctgggat gagcggcctt
     7621 accccgtttt ttacgcgagg taattatgaa gatcaatgac tagccgtcga accgtgtgca
     7681 tgaactgctg cactgaaact tctcagctgt aaaataatcc ttacgctacg tacttctcgg
     7741 agcgcttaca attaatcgga tgcagtcctg actggaagaa agaaaaatgg agaaaacaag
     7801 aggaattaat atcctccacg ttcctgaaat cctggaggat attaatgtca tcaaaatatt
     7861 agcgaacgat aatccctaac aaaataccca gtgcgccaaa gtcagcatca ctgaaggtgg
     7921 tgttagcaaa accaatatca cccagcaccg gcaataagaa tactggcagg aaggtaataa
     7981 gcaagccttg agcgaaggca cccaaaatcg caccacgacg tccacccgtt gcattaccga
     8041 atacccctgc agctgcacct acgaagaagt gcggcaccac accaggaata atcacgatca
     8101 tgttcagcag atacaacgtg aacataccga ttaaacctgc agcaaagcta ctcaggaaac
     8161 caaccagtac agcgttaggt gcgtaaggga agacgaccgg gcaatcgaga gcaggtctgg
     8221 cattcggaac cagcttgtct gaaatacctt tgaatgccgg gacaatctcc gcaatcacca
     8281 tgcgcacacc ctgcagaatg atgtacacgc cagctgcgaa ggtaatggat tgcatgatag
     8341 agaacatgaa ccagttttta ccaccgctaa gctctttcac cgcatctgca cccgcaaaca
     8401 ggcacgtcac caggaaaatt atgctcatgg taaaagagat agcaaccggc gtgtcgcgca
     8461 agaacagcag acttttcggc acattcatgt cttcagtcga gtgctcttta ttaccaaact
     8521 tgctgccgat gaaaccggcc aatacataag ataatgttga gaagtggccg atggcaacgt
     8581 catcagaacc ggtcactttc ttcatgtacg gatgcgcaat agccgggaaa aagaccattg
     8641 ccacgccaac aaccaaagag ccaacggcta taagcgtaat gccggtcatg cctgctgttg
     8701 ccagaattac cgctaccatc atcgacataa acaacgtgtg atgaccggtc aggaagataa
     8761 atttccacgg tgtgaaacgg gcaatcataa tgttgataac catcgcaaag aacataatca
     8821 tcgccatttc tttcccgaag cttttctgcg ctacggaaac aatggcttca ttattcggca
     8881 cgacaccctg aataccaaag gcatgctgga aaatgtttgc gaagtcaccc agtgacgaca
     8941 caactaaact cgcccctgcg cctaaaatca caaaccccat aatggtcttg acggtacctt
     9001 taatgcattc ggttaccggt tttttctgtg caatcaggcc aatcagggcg attaaaccga
     9061 ccagcaccgc gggttcggaa agtacatcgc tcatcaaaaa acgaaagaaa tccatatcgc
     9121 ccccagctta cagtgcgcct aattcggtta atgccacgga caaacgttct ctcatggctt
     9181 tcttatcaat catattttcc agggcgacga ttttgcctgc gacagactgc gcgactaact
     9241 gctcggcaat atctttggtg ccgacataga tatcactcgg cgtccctttc gcggagccca
     9301 ggtcgacatg atcaacatca gcgctgatgg acaaatcctt caaaatgctt ttaatgctca
     9361 tttccatcat caggctggtg cctaagccgt taccgcaaac cactgttatt ttcataaatg
     9421 ttcctcagga ttaatagcgc gcaataacag acaaaatgtc ctgcgtcgtt ttggccgtca
     9481 aaatagcctg tatgtctttt tcgttatcga ataacttggc gagttgagaa atcgcttcaa
     9541 tgtggctggt actgtcggtt gcagccagca caatcagtaa tttcactgga tcgttttcat
     9601 cggcatcaaa attcacacct gaagggatca gggtcagtgc taatgacagc ttgttagccc
     9661 cctcttccgg gcgcgcatga ggcatcgcaa taccagggcc aaccacatag tacggaccga
     9721 tagtgtcgtg agagtgatag atcgcatcaa cataacgcgg ttcgatagaa ccgttttcaa
     9781 tcaacggtcg gcatgaaatc gccacggctt cccgccagtc tttgacctgt tccacgacct
     9841 gaacgacctt tgtatttaat agatttttta acatgcgact gataactccc gatgataatt
     9901 aacaggagag tagatatttt acgcattaat taatgtgatg attgtcgcat ttgatagcgc
     9961 tatctgataa cgctaccaat ctttgtgata gcgctatcat ttctctgtcg ccatgctttg
    10021 gctatattcg gttcctgcat atacaatgca acaggatatt tgctaccgtg ggcaaaaact
    10081 ggaaagagta atggtaaaat aaaagtaatg aaaacagctg gttgtaatta tccatggagt
    10141 cgaaatggaa aaaacacgta agcgccgtgg taccggacgg gtgacgctgc aggaggtcgc
    10201 taactttgca ggtgtcggaa ccatgaccgt ttcccgcgcg ctgagaacac cggaacaggt
    10261 ttctgacaag ctgagggaaa agatagaaca ggccgtagaa gagttgggtt atattcccaa
    10321 cagaactgct ggcgcccttg catctggtca cagtgataca gtcgccgtac tggttccttc
    10381 cctgacagat aaagccagct cccgctttat gcagtcgttg cagcaggtgc ttaataaaaa
    10441 tgaattccag ctactgcttg ggtgtcatga gtataatcag ctcaaagaag ctgaaattct
    10501 gatgacgctt cttcaaggca acccggcggc gttagttatt tttggttctc agttggctga
    10561 taaaactcac cagatactcg agaaaacaaa tatacctacc attaacgttg tcggttcccc
    10621 tttcagtccg gctcaaatta ccatcgaaac agcatttttt gaagcatcac ataaactgac
    10681 cgaacatctg ctggagcagg gttataagaa tatcggattt atcggtgcgc atatggaaaa
    10741 ccgcctgcaa cgtcagcaac tgaacggctg gcataaagca atgctgagcc attaccagaa
    10801 cactgatctt gttatcacaa tgccggatgc cgcaagtctg caattggggc gttacgcatt
    10861 aaacgagata ttactgcgac aaccagagtt ggatgctgtg atttgcagcc acgaagagat
    10921 tgcgctgggt atcatgtttg agtgtcagcg gcgtttgtta aaaatccctg gaaatatcgc
    10981 tgtagcttgt cttgacggtt cagacagttg cgatcaaaca cacccaacct tgacatccat
    11041 ccgtattgac tacaaaaaaa tggggataga gacgggtaag ttactgattg gactattgaa
    11101 taataatcat gatgaatcag aagagtcgcg tatcgtgcaa ttcaactacc agatagaatt
    11161 acgtcagagt acatgagcct aaaagggcac tattgcaaag ttagctattg tggtagggat
    11221 agccgtttcc ggctattccc cgcgcagatc cggatgtgtg ctgctaacgc atccattcct
    11281 acttcaggta tttggcgtag aagcggatgc taaaaacttg ccgcaacacc gcagtatcag
    11341 cccgcagagt cgctctccat ctccagatta tccattccta tattatctct gcaccggatg
    11401 aattacgcga acaactcaga aatatgacac atatgcaact tatcaggact ccgggatcct
    11461 ggaagcctag tgccagtgaa tatcgtaatg ttaccaacgt ttatcgcatt gcattgaaat
    11521 cacttgcctg acgctatttc gagttacatg atgaaatcgc cgaccttgat gtcatgattg
    11581 cggcaattgt cgatgaactg gcacctgaac tgattaaacg taatgctgtt ggatacgaaa
    11641 gcgcnnnnnn nnnntcgcag ttgctcatca ctgccggaga taatccccaa cggctaagaa
    11701 tagaatcagg ttttgcggtg ctgtgtggtg tcaactcggt taccgtatct tctaaaaaaa
    11761 tgaaccgtta ccgacttaat cggggtgggg agcgtgctgc aaatagtgca cttcacatca
    11821 tcgctatcgg acgtttacga actgacgata aaacaaagga atatgtcgct aaatagctgc
    11881 gcctaatacc gctacacttt tgccagacca tgtctcccgc cggggaacgg actggcagtt
    11941 tccataaagt gttggacttt tttcagcaac tgccacatct ccggcactga tgattgcgca
    12001 ttatcgtttc atgaagcgcc tgccatagcc gttcaaaatn nnnnnnnnnt tcaaaatgat
    12061 ttacccatgg cgagtaaact tgctgataaa tcaccctgaa cttcggattt tccttcagcc
    12121 aacgctgcgt ttcccggctt ttgtgaataa tgtaattatc cacgatgagg ggcatcgttt
    12181 ttgcccgccg gtacacagac ttcagttgct tcaacagact gataaataag gctgaacttt
    12241 gcggttactg cccacgtagc agactttgcc cgttcagcta tgtaacgcgc cagcaagata
    12301 atatttttca ttctgccccg gtgtcatcac ccgcttttgc tgtccgcgca attgccagtc
    12361 agcaccggtt ttgggatgga tagccaggtt aacagcgtcc cgataacgtc ttgattctta
    12421 taagggcgtt aactaatttc gttacatcat atacatgatg cttgtgtgcg aagtaacgat
    12481 agacaccaag tagatgctta aaggaagagg ctgacataaa actcaaggat atgaactttg
    12541 cctgcatata ggctacacca tggacgtaat agcgttaaaa aagctactct tactaaacag
    12601 attatacatc agatcgcctc gcagattacc tctatattcc cagaagctcc gcccactgcc
    12661 tgactcagga gtttctcgat aacgactatc cgctctatat gccggataca cgcttgtatc
    12721 acgtgggcct ggcgctgcaa aaaatggcgg ctaactatat tgaaagcatc gatcaggacc
    12781 gcgatttgcg gctattgatc tcatttctga gcaaacggtt gggaaaacca gctacgcggc
    12841 gattctggac gacaggcagg tggtttattt ggcaaaagcg ttctcctatg atgaactctc
    12901 tcattaatgc ggcatatcgg atcggcaata atctcccagc cactgtaccg ccgcttgtaa
    12961 agtgctgctg agccaatatc actttctgat aggcttctga agcgtcatgg gcgttcttac
    13021 gcaataaccc gttataccac tgtgaccaca accagacggt accgccgaag caattaaaca
    13081 accaataatg gtgttgataa gacacggaag gatgtactgc tcgccattca gccataacag
    13141 ttgcagggta tatacagcag taatggtaaa accaaccttc accagccgta gtttttgcac
    13201 aatatcaggc agctggcgaa ggtggtaatc agcatcaaca aggtataacc ttcggaaatt
    13261 ttaaagtgca gcgcaatcac ccgcggtttg tcgtagctca gcgtggcgaa tagttcgtcc
    13321 agcgcgtcaa tagtccccct caccttgcag ggtctggaaa atctagttaa tcgggtactg
    13381 cataacactc tc
//
LOCUS       DAWDRC010000031        13077 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2479_length_13077_cov_2.276225, whole genome shotgun sequence.
ACCESSION   DAWDRC010000031 DAWDRC010000000
VERSION     DAWDRC010000031.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13077)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 13077)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2479_length_13077_cov_2.276225"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1811..1820
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2215..2224
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4577..4586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10381..10390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11304..11313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3211 a         3505 c         3210 g         3101 t
ORIGIN      
        1 aaataatgaa ttcttgctgg caaaaacgcg actggaagcg acgcttgaag cgcgtcgcag
       61 ccaggagatg gactacttca tggaagtatt tgttcccagc ttccatcttt cgctcgatga
      121 gattagccag gattcaccgc tgtaagcagc cggagtctgc gtcgcatcag gcaataagca
      181 ccggatgcga catcaggctc ttgtcaaaac ggaaacagca tcgggatcat caccacacaa
      241 accgccatca cgataatggt gaacggtacc cccaacttaa caaagtcact gaagctgtaa
      301 ttccccggac ctaaaaccag tgtgttaaca ggtgaagata tcggtgtcat aaaggcggcg
      361 gatgctgcca tcgccacgac catcgcaaat ggataaggcg acacccccat cgttttggca
      421 gcagccagcg caatcggagc catcaacacc gccgtcgcgg tattagaaat aaatagccca
      481 ataaccgccg acaagacaaa caaacaaccc agcatcatat gtggcccgta accgccgcca
      541 atgtccatca gccctttcac cgccagcgcg acacctcccg ttttctgtaa tgccacagca
      601 aatggcatca tcccaacgat caaaataatg ctcggccagt gaatggattt ataggcgctt
      661 tcagcatcta tacagcggaa tttccccatc agcaggcagg cgataatagc ggcaacagga
      721 ttaggaattt catcggtcag cattaacgcc accattagca ccagacagaa aatcgcatgg
      781 ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt ccggtaagtt cagcgctacg
      841 aagtcgcggc cctgtttggc cagcataccg atcagtttcc agttacccac aaccaggatg
      901 atatcgccca gcagcagagg ctcatccgcc agcgaacctt ccagcgccac gccattgcgc
      961 tttagcccca ctacattcaa tccgtagcgg gtacgaaaac caatttcgcg caccgattta
     1021 ccaataagtt ctgactcagg aattaatgaa atctctgcca tgcccacatc aagggcctgg
     1081 tcagaaaaat actcgccgcg cagtaccatc ggctccagca attgctcact acaaaattgc
     1141 cggagatcga catcagccgc agacatatca ataagcaaaa cgtcacgcgc gcgaaattca
     1201 gaaaccccat taacgttcac gataacgcga cgaaaacgcc gccagcgttc aacaccgatg
     1261 acgttagcac cataacgttc acgtaatttg agatcgtcca gccgttgacc aatcattggc
     1321 gatccggggc gaatagccag acgtcgcgca cgcccggtca gtcgatattc acggataaga
     1381 tcgcgaaagg ttcggcgcgt ccagccttcg cgctgcgggg tctgggtatc ccctttcagc
     1441 atgaaacgca tcactaacat atacaaaata cccagtacca gcacgaccag gccaataggt
     1501 gttacgctaa agaaactaaa accatgatag ccttcacgca gcaattcact gttgactacc
     1561 aggttcggcg gcgtcgccac cagcgtcatc atgccgctta tcagcccggc aaaacttaac
     1621 ggcatcatca ggcgcgacgg cgacgtttgc atacgcatgg caacgcttaa caccacggga
     1681 ataaagatag cgacaacgcc ggttgaactc ataaacgcgc caagccccgc gacggtcagc
     1741 atcaacaaaa ccaacatttt gatttcactg ttgcccgcca ctttgacccg ccatggcccc
     1801 attacagtgg nnnnnnnnnn acgttaggat cggaaaagcc ggaaaatact tcggggaccg
     1861 tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca tccatacgca
     1921 ctctgcccgt cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc caaatcaatt
     1981 caccgttcac aacttatcct tgttaattga gggggataac ttgattctgc cataaaaaag
     2041 ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa catcgccatt
     2101 tggctgtttg gtcacagtaa tttgctccag actgtggagg actaaatcga cttcattcag
     2161 gcgcggggta tctgccgggg cattaaccgc aataacatgg caacccgccg ccagnnnnnn
     2221 nnnnggagca tcttccacca caacacactc ctgcggcgca agccccagca gctgcgcgcc
     2281 taacagatac gcatcaggtt ctggttttcc gcgcttcacc cgctcagcgg ttacaaacac
     2341 ctctggcgcg ggaagccccg ctattttatg acgcgctcgc gctaccggca tggagccaga
     2401 agtcacaata gcccacggaa tacctgcttt attcaaatga ttgagtaagg cgattgcccc
     2461 cggaagcgcg gtaatacctt cggtttccgt ggcctcgatg tgctccagac gcgtaaactc
     2521 ggcggcaatg tctgcctcgg atttgcccgc cataaaatgg cgcagagagg tgatcgcctg
     2581 tttaccgtga atgaaagcca gcacctcttc cggcgataac ccatgacgtc tggcccagtt
     2641 gctccacgcc cgttctaccg caggcaggga atccaccagc gttccatcaa gatcaaacag
     2701 aaaacctttg caccgcacgc ggggcctcct caggcgttaa taatttgatt aatttcgttg
     2761 gcgctcaaat ggtactgacg cgggcaggca tgccacacat taagcatgcg ctgatatttt
     2821 tcccacattg gcgtttgggc gttaaaaccg tgagttccag cgtcaaaatg ggtatagcgc
     2881 ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca
     2941 aagccgagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa attagaccag
     3001 gaaacatgca acgcatgata catctccata atgtcgatga tagtgcggca ggtttcttct
     3061 ttcagctcgc caaactcgcg atccagttca cgcatctgta atccataacc acgctcaatg
     3121 attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt catcatcttg
     3181 tactggttag acaaaataag acgttgcgcg ttggtcattt ccattattga ctcctgtatc
     3241 actctactac ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg cgtaatcagg
     3301 ggtcaattac aaatcatcaa ggaaagtttt atccagttgt ttgaaggcac gcttaagcgt
     3361 gtcagctaat gcctggtaat caggcttgcc ttcaacgggt gccaacacct gtccagactc
     3421 ctgcaattta ccgcgaactt cataaaacca gttgaggatt gcaggaggta atggcgttac
     3481 agaacgcttg cccagccacc acaatccctg catgggtaaa cttaaggcga acagcgcagt
     3541 ggcaactgcc ggcccaagct gaccgcccag ggcaatctgc cagcagagag taaatacggc
     3601 gatcggcggc ataaaacgga tcgcataaca cgtcatcttg ataacgcgat tttcgacaaa
     3661 gactggggca aggcgttttt ccagcggcca cgtctttgag taatgctgtc cccggcgaaa
     3721 caagctaaaa aaattaacag aacgattatc cggcgttgac atgcttcacc tcaacttcac
     3781 atataaagat tcaaaaattt gtgcaaattc acaactcagc gggacaacgt tcaaaacatt
     3841 ttgtcttcca tacccactat caggtatcct ttagcagcct gaaggcctaa gtagtacata
     3901 ttcattgagt cgtcaaattc atatacatta tgccattggc tgaaaattac gcaaaatggc
     3961 atagactcaa gatatttctt ccatcatgca aaaaaaattt gcagtgcatg atgttaatca
     4021 taaatgtcgg tgtcatcatg cgctacgctc tatggctccc tgacgttttt ttagccacgt
     4081 atcaattata ggtacttcca tgtcgagtaa gttagtactg gttctgaact gcggtagttc
     4141 ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa gagtaccttt ctggtttagc
     4201 cgaatgtttc cacctgcccg aagcacgtat caaatggaaa atggacggca ataaacagga
     4261 agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg ttaatactat
     4321 tctggcacaa aaaccagaac tgtctgcgca gctgactgct atcggtcacc gtatcgtaca
     4381 cggcggcgaa aagtatacca gctccgtagt gatcgatgag tctgttattc agggtatcaa
     4441 agatgcagct tcttttgcac cgctgcacaa cccggctcac ctgatcggta tcgaagaagc
     4501 tctgaaatct ttcccacagc tgaaagacaa aaacgttgct gtatttgaca ccgcgttcca
     4561 ccagactatg ccggaannnn nnnnnntacg ccctgccgta caacctgtac aaagagcacg
     4621 gcatccgtcg ttacggcgcg cacggcacca gccacttcta tgtaacccag gaagcggcaa
     4681 aaatgctgaa caaaccggta gaagaactga acatcatcac ctgccacctg ggcaacggtg
     4741 gttccgtttc tgctatccgc aacggtaaat gcgttgacac ctctatgggc ctgaccccgc
     4801 tggaaggtct ggtcatgggt acccgttctg gtgatatcga tccggcgatc atcttccacc
     4861 tgcacgacac cctgggcatg agcgttgacg caatcaacaa actgctgacc aaagagtctg
     4921 gcctgctggg tctgaccgaa gtgaccagcg actgccgcta tgttgaagac aactacgcga
     4981 cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca ccgcctggcc aaatacatcg
     5041 gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt tgtattcacc ggtggtatcg
     5101 gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa actgggcgtg ctgggctttg
     5161 aagttgatca tgaacgcaac ctggctgcac gtttcggcaa atctggtttc atcaacaaag
     5221 aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga actggttatc gcgcaagacg
     5281 cgagccgcct gactgcctga tttcacaccg ccagctcagc tggcggtgct gttttgtaac
     5341 ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc cgtattatta tgctgatccc
     5401 taccggaacc agcgtcggtc tgaccagcgt cagccttggc gtgatccgtg caatggaacg
     5461 caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag ccgcgtaccg gtggcgatgc
     5521 gcccgatcag actacgacta tcgtgcgtgc gaactcttcc accacgacgg ccgctgaacc
     5581 gctgaaaatg agctacgttg aaggtctgct ttccagcaat cagaaagatg tgctgatgga
     5641 agagatcatc gcgaactacc acgctaacac caaagacgct gaagtcgttc tggtggaagg
     5701 tctggtcccg acacgtaagc accagtttgc ccagtctctg aactacgaaa tcgccaaaac
     5761 gctgaacgca gaaatcgtct tcgttatgtc tcagggaact gatactccgg aacagttgaa
     5821 agagcgtatc gaactgactc gcaacagctt cggcggtgca aaaaacacca atattaccgg
     5881 cgttatcgtt aacaaactga acgctccggt tgatgagcag ggtcgtaccc gtccggatct
     5941 gtccgagatt tttgacgact ccaccaaagc aaaagtgaac aacgttgatc cggcgaagct
     6001 gcaagaatcc agcccgctgc cagttctcgg cgctgtgccg tggagctttg acctgatcgc
     6061 gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc atcatcaacg aaggcgacat
     6121 caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc agcattccgc acatgctgga
     6181 gcacttccgt gccggttctc tgctggtgac ttccgcagac cgccctgacg tgctggttgc
     6241 cgcttgcctg gctgccatga acggcgtaga aatcggtgcc ctgctgctga ctggcggcta
     6301 cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct ttcgctactg gcctgccggt
     6361 atttatggtg aacaccaaca cctggcagac ttctcttagc ctgcagagct tcaacctgga
     6421 agttccggtt gacgatcatg agcgtatcga aaaagttcag gaatacgttg ctaactacat
     6481 caacgctgac tggatcgatt ctctgactgc cacttctgag cgcagccgtc gtctgtctcc
     6541 gccagcgttc cgttatcagc tgactgaact tgcgcgcaaa gcgggcaaac gtatcgttct
     6601 gccggaaggt gacgaaccgc gtaccgttaa agcagccgct atctgtgctg aacgtggtat
     6661 cgcaacttgc gtactgctgg gtaatccagc agagatcaac cgtgttgcag cctctcaggg
     6721 tgtagaactg ggtgcaggca ttgaaatcgt tgatccagaa gtggttcgcg aaaactatgt
     6781 tggtcgtctg gtcgaactgc gtaagaacaa aggcatgact gaaaccgttg cccgcgaaca
     6841 gctggaagac aacgtggttc tcggtacgct gatgctggaa caagatgaag ttgatggtct
     6901 ggtttccggt gctgttcaca ccaccgcaaa caccatccgt ccgccgctgc agctgatcaa
     6961 aactgcacca ggcagctccc tggtatcttc cgtgttcttc atgctgttgc cggaacaggt
     7021 ttacgtttac ggtgactgtg cgatcaaccc ggatccgact gcagaacagc tggcagaaat
     7081 cgcgattcag tccgctgatt ccgctgcggc cttcggtatc gaaccgcgcg ttgctatgct
     7141 ctcctactcc accggtactt ctggtgctgg tagcgacgta gaaaaagttc gcgaagcaac
     7201 tcgtctggcg caggaaaaac gtcctgatct gatgatcgac ggtccgctgc agtacgacgc
     7261 tgcggtaatg gctgacgttg cgaaatccaa agcaccgaac tctccggttg caggtcgcgc
     7321 taccgtgttc atcttcccgg atctgaacac cggtaacacc acctacaaag cggtacagcg
     7381 ttctgctgac ctgatctcta tcggaccgat gctgcagggt atgcgcaagc cggttaacga
     7441 cctgtcccgt ggcgcactgg ttgatgatat catctacacc atcgcgctga ctgcgattca
     7501 gtctgcacag cagcagtaat ctcgtcatca tccgcagctt tgcgctgcgg atatctgaac
     7561 cggaaatagt cactatttcc ggttttttat tctcttaatt tgcattaatc ctttctgatt
     7621 atcttgctta actgcgctgc atcaatgaat tgcgccatct cactttgcat acttaccact
     7681 ttgttttgtg caagggaata tttgcgctat gtccgcaatc actgaatcta aaccaacaag
     7741 aagatgggca atgcccgata cgttggtgat tatctttttt gttgccattt taaccagcct
     7801 tgccacctgg gtagttccgg tgggcatgtt tgacagtcag gaagtgcagt atcaggttga
     7861 tgatcaaaca aaaacacgca aagtcgtaga tccacactca tttcgcattc tgactaacga
     7921 agcaggcgaa cctgagtatc accgcgtaca gctgttcacg acaggcgatg aacgcccggg
     7981 tctgatgaac ttcccgtttg aagggttaac ctcaggatcg aaatacggga cagccgttgg
     8041 catcatcatg tttatgctgg tgattggcgg cgcgtttggc attgtgatgc gtacaggaac
     8101 cattgataac ggtatcctgg cgcttattcg ccatacccgc ggaaatgaaa ttctctttat
     8161 tcctgcgctg tttattctgt tttcacttgg cggcgcaata tttggtatgg gagaagaggc
     8221 cgtcgccttt gccattatca tcgcaccgct aatggtccgg ctgggctatg acagtattac
     8281 caccgtcctt gtgacctata ttgccacgca aatcggtttt gccagttcgt ggatgaaccc
     8341 gttttgtgtg gttgttgctc agggtattgc cggcgttccg gtgctttctg gctccgggtt
     8401 gcgcatcgtg gtgtgggtta tcgccactct gattggcctg atctttacca tggtgtacgc
     8461 ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat gagtccgacc gcttctttcg
     8521 tgaaaagcag gcggatgttg aacaacgtcc gtttaccttt ggtgactggc tggtattgat
     8581 tgtcctgacc gccgtaatgg tctgggtgat ttggggcgtg atcgttaatg cctggtttat
     8641 tccagaaatt gccagccagt tcttcaccat gggtctggtg attggcatca tcggcgttgt
     8701 tttccgcctt aacggcatga cggttaatac catggcttca tcctttaccg aaggggcgcg
     8761 aatgatgatc gcccctgccc tgctggtggg tttcgccaaa gggattttgc tgctggtcgg
     8821 taatggtgaa gcgggtgatg ccagcgtgtt aaataccatc ctcaacagca ttgccaatgc
     8881 cattagcggt ctggacaatg cggtcgcggc ctggtttatg ttgctcttcc aggcagtatt
     8941 taatttcttc gtgacgtccg gttctggtca ggcggcgtta accatgccgt tactggcacc
     9001 gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg ctggcgttcc agtttggtga
     9061 tggcttcagc cacatcattt acccgacctc agcttcgtta atggcaacgc tcggtgtttg
     9121 ccgggtggac ttccgtaact ggctgaaggt gggcgcgaca ctgcttggac tgctgtttat
     9181 tatgtccagc gtcgtggtga tcggcgctca gttgatgggc taccactaaa aatgttaaga
     9241 gccgcaattg cggctctttt tttcattctg ccgtttcagt ctctacggct tcatttttgg
     9301 cattgcgctt catccacaac gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc
     9361 gtgcggtgat ttcttccggc gtcagccagc aaacttcact gacttcatct tcctgtagag
     9421 cgaagggacc gtgagagacg cagctgaaca acgcgcccca gacacggcaa tttttatctt
     9481 cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac tcttcttccg
     9541 cttcgcgacg cgcggattcc agcagttgct catcggcctg gactacaccg cctgcggtcg
     9601 catctaacat gccgggtaaa aagtcttttg tctcggtacg acgctggacc agaattttgc
     9661 ccatgccatc atgcacgacg atataagttg cacgatgacg cagacactgt gcccgcattt
     9721 gttcccggct ggcttgtgca atgacttcgt tctcttcatt gacaatatcc acccattcag
     9781 tacttgccaa acgacgctgt tccattatca ggaaaccttc tttttctggc gctcttacgg
     9841 cgcattttga gttgtgggta aattacggat taatcgcgac ctgcgcaatg atactttgat
     9901 cattgagtgc gattacacta agtacgtcat tatccagcat gccataactc gccggattac
     9961 cgcctttcgg aatactcacc gagccggggt tgaagtggaa aatttccccc cgctgttctg
    10021 ccactggcag atgggtatga ccgtacacca gcacatcgtt ctggtttaaa gcaggtagat
    10081 tttccgggcc aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc agtaatacct
    10141 gttgccacgg cgcggttatc gggaaatgca gcagcatttg atccacttcg ctgtcgcagt
    10201 tgccgcgcac agcgataacc ttatgtgcca cttcattaag ccgttcagcg actttggctg
    10261 gcgcgtaacc ctccggtaaa gcattacgcg ggccatgatt caacacgtcg ccaaggatca
    10321 ccagccactg ggcaccgctt tgggcaaaca actccagaac acgttccgtc gccggtaacg
    10381 nnnnnnnnnn cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt gctatcagcc
    10441 agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc actggaatgt
    10501 cgcataatcc accaggcgtt ccggcacctc atcgccatgt agtaccaggc ggttaatcat
    10561 tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct gacccagtgc
    10621 taacaaatgt tctgccatcg cgaacaattt ctctgcactg gcttttccct tagtggcgct
    10681 tttttcgccc ccgcaaagac aacatccgtc gggcgttctt cgcgaatggg catcagatcg
    10741 ctgcgcagcc aggcctgaat ctgtcgtgct cgcgcacgat tttctaaatc aagcggataa
    10801 atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat
    10861 tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct
    10921 tgccacgtcg gctgcaaatg ttcaccgctg tcgaggttga tggtcttgat atgaaacgac
    10981 aggcctttct cctgcaacgc cacccaggcg gataacacat aaggggagaa aaaatgggca
    11041 tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggcttgc
    11101 tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgc aaatcgccgc
    11161 gctttctata cttgtggtgt tcagatgatt agacaaccgg agctgcaatg atcgatctct
    11221 atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagggccgg
    11281 aatatcgcct gataaaggta gacnnnnnnn nnngtttcgc ccggaatttt tgcgcatttc
    11341 gcctaacaac aaaattccgg caattgttga tcattctcca gccgatggcg gcgaaccgct
    11401 aagcctcttt gagtctggtg ccattttgtt gtatctggct gagaaaacgg gactcttttt
    11461 gagtcatgaa acgcgtgaac gcgccgtcac attacagtgg ttattctggc aggtaggcgg
    11521 actggggccg atgcttgggc aaaatcatca ttttaatcac gcagcccccc aaaccattcc
    11581 ttacgctatt gaacgttatc aggttgaaac tcagcgtctg taccatgtac tgaacaagcg
    11641 gctggaaaat tcgccctggc tgggaggcga gaactacagc attgcggata ttgcctgctg
    11701 gccgtgggtt aatgcctgga ctcgccagcg aattgacctc gcaatgtatc cggcagtcaa
    11761 gaactggcat gagcggatcc gttcacgccc tgccaccggg ctggcactgc taaaagcaca
    11821 acacggtgat gagcgttcgg atagttaaca gaagcaggtt ctcgtgtatt atttcattct
    11881 aagtaaaaca acggagaacc tgcaatggca caacctgccg ctattattcg tataaagaac
    11941 cttcgtttac gtacgtttat cggaattaag gaagaagaaa ttaacaaccg tcaggatatt
    12001 gttatcaatg tgacgatcca ctaccccgcc gataaagcgc gcactagcga agatatcaac
    12061 gatgcgctga attatcgcac cgtaacgaaa aacattattc agcatgtaga gaataaccgt
    12121 ttctctttgc tggaaaaatt aactcaggat gtgctcgata tcgcacgtga acatcactgg
    12181 gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta
    12241 tccatgacct taagctggca gcgttaaccg ccatatcggg aggctgcatg aacatagtga
    12301 tcaccggagg gacgggatta attggtcgcc atttgattcc acgtttgctg gacctgggcc
    12361 atcaaatcac ggtagtgacg cgtaacccgc agaaagccag ttccgttctc ggccctcggg
    12421 tgacactatg gcaagggctt gccgaacaaa gcaacctcaa cggcgttgat gcggtaatca
    12481 acctggccgg agaaccgatt gctgataaac gctggactca cgagcaaaaa gagcgtctct
    12541 gccaaagccg ctggaatatc acgcaaaaac tggtcgattt gattaatgcc agcgacacgc
    12601 caccgtcggt actcatttcc ggctcggcaa cgggctatta tggcgactta ggtgaagtgg
    12661 tggttaccga agaggaaccg ccgcataacg aatttaccca taaactctgc gcccgctggg
    12721 aagagattgc ctgtcgggcg caaagtgaca aaacgcgagt gtgcctgctg cgtaccggcg
    12781 tagtgctggc accagacggt ggtatcctcg gtaaaatgct gcggccattt cgttttggcc
    12841 tgggcgggcc gattggctct ggtcggcagt atctggcctg gattcatatt gatgatatgg
    12901 tcaacggtat tctctggctg ctggataacg agctgcgcgg gccatttaat atggtttcgc
    12961 cctacccggt gcgcaatgaa caatttgccc atgcactggg tcatgcgctg catcgcccgg
    13021 ccattttgcg cgtccctgcg accgccattc ggttgttaat gggcgaatct tcagttc
//
LOCUS       DAWDRC010000032        12810 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2546_length_12810_cov_2.394198, whole genome shotgun sequence.
ACCESSION   DAWDRC010000032 DAWDRC010000000
VERSION     DAWDRC010000032.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12810)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12810)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2546_length_12810_cov_2.394198"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1657..1666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3299..3308
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3692..3701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4010..4019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4936..4945
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5545..5554
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9396..9405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10845..10854
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12563..12572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2856 a         3430 c         3282 g         3152 t
ORIGIN      
        1 attcactacc atttagggga ttatgctgga tatttttcat tctctaatgt tttaactttg
       61 taattattgc tgaaaaaaaa ttaatcacct gccaaaagaa ataaaaaaga gaaagcctcc
      121 gattaaatta tttcgctaca ctggttccac ttttgtgatt tacacgggtt ccccatgaag
      181 ctgaccatca ttcgattaga aaactttagc gaccaggacc ggattgacct gcaaaagatc
      241 tggccggagt atttcccttc ctctttacag gttgacgata accaccgtat ctacgccgcg
      301 cgttttaacg agcgcctgct cgctgccgtg cgggtaacct taagcggcac cgagggggca
      361 ctggattctc tacgcgtgcg ggaagtcacc cgacgtcgcg gtgtggggca atatctgctg
      421 aaagaggttt tgcgtaacaa tcctgacgtt tcatgttggt ggatggcgga tgctggcgtg
      481 gaagatcgcg gcgtgatgac ggcgtttatg cgagcactgg gatttacggc acaacagggt
      541 ggctgggaga agcattaatc gtcaagtttg atttcaaaag tgatattgcc tgatgcgcta
      601 cgcttatcag gcctacaatg tgtgctgcaa tttactgatt tctttgaatc ttgtaggccg
      661 gataaggcgt ttacgccgca tccggcatga agcaacgtac tcgatattag caatttggcg
      721 gcaacccaaa gttgccgatt aatgattact tcgcatcagt cgccgtgccg ttggcgtgcc
      781 agtcaaacac accgaactca aagcctttca gatcgccttt ctcatcccag gtcagcggcc
      841 ccatcaccgt atcaaccgag ttcgctttca ggtatttagc gatctccgcc ggatcgtcag
      901 actgattcag ccccgcttgc aaagattgca gcgcagcgta ggtggtccag acgaacgcgc
      961 cgcttgggtc ctgttttttc gctttgatcg cgtcaacaat cggtttgttc gccggaacct
     1021 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
     1081 acagcgagac gttagccacg ccttctggcc ccataaactg agttttcagc cctgccgcgc
     1141 gtgcctgacg caggatttgt cccatttccg ggtgataacc gccgtagtaa acgaaatcga
     1201 tattctcttt cttcaaacgc gccaccaggg tagagaaatc tttttccccg gcggtgatgc
     1261 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcact gcacgcgcca
     1321 gaccttcgcc gtactgctgt ttgtcgtgaa cgatagcaat gcgttgcggt ttcactttct
     1381 caagtatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
     1441 tcagctgata gccacgggcg gtcagctctg gtgcggttgc cgctggggtg atcattaaaa
     1501 tgccttcgtc ttcgtagatg tcagacgctg gctgcgttga tgaagagcag aggtgaccaa
     1561 tcacgtattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
     1621 cacaggcatc gtcatatttt acgatttgca gtttgtnnnn nnnnnnccac ctttagcgtt
     1681 aatatccgca actgcctgct ctgcgccagt aaactcctgg tcaccgtact gcgccaccgg
     1741 accggacatt gcacctacga cggcgacttt aatatcttct gccagagcca tattgctgaa
     1801 tgccagcgcg atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc
     1861 cactcttctg gttattacgt gtgttgtgat gttgttgtgc agcactttat ttcgttttat
     1921 acgtagctac ccgtgcttta gcagcatact ctgctaaaac ataccccatt tttatgatat
     1981 tggaatagct attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag
     2041 ggggaattga ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc
     2101 tctgcttaat agcggaaatt acgcttcaat agcagcgcgc aattttttca tcgcgttctt
     2161 ttcaagctga cgcacacgct cagcggaaac accgtaacgg tcagccagtt cctgcaacgt
     2221 ggacttgttg tcttcatcca gccagcgcgc gcggatgata tcctggctac gctcgtccag
     2281 accttgcatc gcatcggtca ggcggtttgc cgcctgctct tcccagttat catcttcaat
     2341 gccatcggca aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct
     2401 gtcggaatcg tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc
     2461 catctcacgt acgtctttgc tggttacgcc cagttcacgg gccaccatct cgacttcatc
     2521 ctggttaaac cggcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgttg
     2581 cgctttggtg gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc
     2641 tgctttgatc cagtgaacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg
     2701 gcgcactgct ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc
     2761 atagcccgca taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt
     2821 tttagctgct tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc
     2881 agccgacaac atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg
     2941 ggctaaagct aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg
     3001 gcgcttcagt ggtagcaaca actgtgccag agcttaagag caacgaggtt atcattcact
     3061 gttttatcag accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca
     3121 caaaatgtga catagagata aaataccggg aagagacaac ggggtctctt tccctgctac
     3181 ggaacccatt gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta
     3241 aatgttgtac cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagnn
     3301 nnnnnnnnaa tagcaggcat tcatcgaacg ataagccatt gatatcaaac ttcgttccga
     3361 aaacctgtgc cacttccgca accgccgatg acaaacgcag caccagaatt tctgacaaaa
     3421 ttagtgacaa caatgcgcca gaaaatccca gcaatgcgcc accgtacagg aacggacgca
     3481 ggatgaaacc atccgtcgca ccaatcagtt tctgcacgtt aatagaatca cggcgagcaa
     3541 agatactcag gcgcacacta ttaccgatga cgaggaacac tgccgccacc atcaatacgc
     3601 cgatcattgc cgaaacgcgc ccgaccagcc cggtcaacgc cgccaggcgg gcaaaccagc
     3661 tgtcatccat ccgcacttcg tcaatgccgt tnnnnnnnnn natcacgcag cgtattcagt
     3721 gactccgtcc cctgaaaatc gagtttcggg atcaccaccg ccactgccgg aagcgggttt
     3781 tcttccagca tatccagcgc accaccaaaa ccagaccagt tacggaactc gcccagtgcg
     3841 tcttcacgag aaagataatt cactttctcc acgccttgct cggcctgcaa ctgtgccacc
     3901 acgcccgcag cggcgtcatc atccagcgtt ttttgcagat aaacagtgat ttgcggtgac
     3961 ggataatatt gcgtcgccgc ctggttaacg tttttgtaca ccatataacn nnnnnnnnna
     4021 tataacagac gctgggcagc gtcagggaga tggcgataac catcaccgtt aaaaacgtgg
     4081 cgaacggttt gcttttcaga tcctgcaatg cgccgtggaa ggcatagcgt acctgttcgt
     4141 tgaaaacgtt ggttttgcga tttaccggtt ttggcgagga tttcgcgcgt ttcggcgcgt
     4201 tacgaccgcc gtcgccagag ccgccgaccg atttacggaa gcgatccaga cgcccgccaa
     4261 actgccgaat gtgattgatt gcatcgcgct tattcatggc ccacgcctcc atgcaagtga
     4321 ccatcgctca gggtgagcat gcgataggaa cgtcgcgaaa tcaggttgat gtcgtgcgtt
     4381 gccatcaata cggttactcc aacgcggtta aactcttcaa acagacgtaa aatgccttcc
     4441 gacagcgcgt cgtccaggtt accagtcggt tcgtccgcca gcagtaccgc gggcttgttc
     4501 accaccgcgc gggcaatgcc aacacgctgt tgttcaccgc cggaaagctg aatagggaag
     4561 ttcttcgctt tgtccagtag cccgacttta tccagcgccg ccgacacccg gcgacgaatg
     4621 tcatcaccgc tggcaccggc gataatcagc gggatcgcca cgttatcata gacagtacgg
     4681 tccatcagta gatggtgatc ctggaaaatc atgccaatct ggcggcgcag aaacggaact
     4741 tcacggtttt tcagacgcgt gatgtcatgg ccgctaaacc agattttccc ggcgctgggc
     4801 cgctcaatcc cacagatcag cttcaggagg gtacttttcc ctgcgccgga atgaccggtc
     4861 agaaacgcca tctcacccgg ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca
     4921 ccgagataag ccttgnnnnn nnnnnaatca ttgttaatcc tctcgggcaa aaagtgcctc
     4981 tataaagtcg tccgccttaa acggacgcaa atcctcaata cgttcgccga caccaatgta
     5041 gcggataggg ataccaaact ggtcagccac cgagaaaatt accccgcctt tcgccgtgcc
     5101 gtccagtttc gttagcgtga tgccggttaa gccaacggct tcgtggaaca gtttggcctg
     5161 gcttaccgca ttctgcccgg tgctggcatc gatggtcagc ataacttcat gcggagcttc
     5221 aacgtcgagt ttcttcatca cgcggacgat tttcttcaac tcttccatca ggtgcgattt
     5281 gttttgcagg cgtccggcgg tatcggcaat caggacgtcg atgttacgcg ctttagcggc
     5341 ctgaatggcg tcgaagataa cagaagcgga atcagcaccg gtatgctggg caatcaccgg
     5401 aatattgttg cgctgacccc agacctgaag ctgttcaacc gctgccgcac ggaaagtatc
     5461 gcctgccgcc agcatcaccg atttaccctg ctgctcaaac tgacgcgcca gcttaccaat
     5521 cgtcgtggtt ttacccacac cgttnnnnnn nnnngccata gagcgcctcg gcatcacgaa
     5581 gctgcttgcg ggtcgcacct tctgtcagat tggtgataat tttacgcgtg gtttccacgc
     5641 ccacatcggc gatgagcaat tgctcttcca attcttcaaa cagatcatcg tcgatttttt
     5701 taccgcggaa caggctgata aatccggaac cgagattttc tttggttttt aacaggctgc
     5761 gtttcaggcg cgcgaagaaa ccttctttgg tcggtttttc ctgctcctga gcgatttctt
     5821 ccaccggctg atcttcttct gccggagaaa ccaccattac cgcctcttct gccacttcgg
     5881 cagccagcgc ctgtgcctcc ggctcttcgt cggtgatttc ttctttagcc gcttcttctt
     5941 ccgccgcttc gacaatctct acggtttccg cttcggcctg ccactcttct ggcgaaaccg
     6001 cttcggcgtt gacgtcttcc ggcaacggca gttcttcacg ttcgatagcc actggctccg
     6061 gcgtttcttc tacgaccggt tccggctgtg caacgacttc cgcttcaggc tgcgcttttt
     6121 cactttcagc aacctgttca gtaacgtcca caacgttggc agcaaaagtt tccgcctcgg
     6181 cttcagtatg cgcctgcggc tgctcttcaa cggcgtgttc agaagccttc acaggctctt
     6241 gcgcctgaac gatttcttct acaactggtt gttcattctg aacttctgtc tctttttccg
     6301 gggtctgctc tttttgacca aagcccagcc aggaaaaaaa gccacgtttt ttttctttcg
     6361 ccatttgcga ctacactcct cgctgttgct tcatggcaca gcgttaacgc tatgtacata
     6421 gcagctaaaa aaatgatgaa atagtctatc acttaactta attcacatca ccgcctgcaa
     6481 gtatgtgtta tctggcggat tgagcaattt atcatgaaaa aaccgaatca ttccggcagc
     6541 ggccaaatcc gcattattgg cgggcagtgg cgaggccgta aactcccggt tcctgatagt
     6601 ccaggtctgc gccccaccac cgaccgcgta cgcgaaacgt tgtttaactg gctggctccg
     6661 gtcattgttg acgcccaatg tctggattgc ttcgccggga gcggcgcgct ggggctggaa
     6721 gcgttatcgc gctacgctgc gggggccacg ttgattgaga tggatcgtgc ggtttctcag
     6781 cagttaatta agaatctggc gacactaaaa gcaggcaatg cacgcgtggt gaacagcaac
     6841 gcgatgtcat tcctggcgca aaaaggcaca ccgcataata tcgtgtttgt cgatccaccg
     6901 ttccgccgtg gcttgttaga agagacgata aatttactgg aagataacgg ctggctggct
     6961 gacgaagccc tgatttatgt cgaaagcgaa gtcgaaaacg gtctgcccac tgttccggca
     7021 aactggtcat tacatcggga aaaagtggcg ggtcaggtgg cttatcggct gtatcaacgc
     7081 gaagcacaag gagaaagtga tgctgattaa tattggtcgt ttgttaatgc tctgtgtttg
     7141 gggattttta atcctcaacc tggtgcatcc cttcccacgc ccgctgaata ttttcgttaa
     7201 cgtggcgctg atttttaccg tgctgatgca tggtatgcag ctggcgctat tgaaatccac
     7261 tttaccgaaa gatggcccgc agatgaccac cgccgaaaag gtgcggattt tccttttcgg
     7321 cgtgtttgaa ctgctggtct ggcagaagaa atttaaagtt aaaaaataac cttattgttc
     7381 gcctacaaag ctgacaaagc gcgttccttt atagctcagc gtacctttat cacccactgt
     7441 cagggcgtgg tactgctggg cgtcgaggcg aaacgtctgc tccattcctc cgctttgcgg
     7501 tttgaagctt gcctcatagc gcatactggt gcctgccgga gtcacttcct gctggcgcga
     7561 acggcgatcg ttaatcggtt tttcccgctt gttgctcacc accaccagct tttgctggag
     7621 cggtgccata tcattatcag ctttttcccg tcgttgttgc ataaaacgaa acgatgcggc
     7681 gacgatgatt aagccaatga taacaataaa gaaaagaggt ggtttactca tctttatccc
     7741 tcatcggaaa atgcggaaat aagcataccc tgccagttat ggtgttgtca tccgtccacc
     7801 ctcgccacta aactggaaac aagaccgtag gcattccgct tacgaaaaaa taacgaattc
     7861 aaggaactaa gatgctttgg tcgtttatcg ctgtctgtct ttccgcatgg ctatctgtgg
     7921 atgcatcgta tcgtgggcca acctggcaac gctgggtgtt taaaccgtta acccttcttc
     7981 tcctgctgtt gctggcctgg caagcgccga tgttcgacgc cattagctat ctggtgctgg
     8041 cggggctgtg tgcctcactg ctgggcgatg cgctaaccct gttgccacgt caacgtctga
     8101 tgtacgccat cggcgcgttt ttcctctcgc acctgctgta caccatctat ttcgccagtc
     8161 agatgacgct ctctttcttc tggcctctgc cgttggtgct gctggttctg ggcgcgctgt
     8221 tactggcgat tatctggacg cgtctggaag agtatcgttg gcctatctgc acgtttatcg
     8281 gcatgacgct ggtgatggtg tggctggcag gtgaactgtg gttcttccgt ccgaccgctc
     8341 cggcgctctc tgcgtttgtc ggcgcttcgt tgctgtttat cagtaacttt gtctggctgg
     8401 gtagtcacta tcgccgacgc ttccgtgcgg ataacgccat tgccgcggcc tgctactttg
     8461 ctggtcactt cctcatcgtc cgctcgctgt atctctgata aaacttgact ctggagtcga
     8521 ctccagagtg tatccttcgg ttaatgagaa aaaacttaac cggaggatgc catgtcgact
     8581 cctgacaatc acggcaagaa agcccctcaa tttgctgcgt tcaaaccgct aactacggta
     8641 cagaacacca acgactgttg ctgcgacggc gcatgttcca gcacgccaac tctctctgaa
     8701 aacgtctccg gcacccgcta tagctggaaa gtcagcggta tggactgcgc cgcctgtgcg
     8761 cgcaaagtgg aaaatgccgt gcgccagctt gcaggcgtga atcaggtaca ggtgttgttc
     8821 gccaccgaaa aactggtggt ggatgccgac aatgacatcc gggcacaagt tgaatctgcg
     8881 gtgcaaaaag cgggctattc cctgcgcgat gaacaggcct ccgacgaacc acaagtatca
     8941 cgcctgaaag agaatctgcc gctgattacg cttatcgtga tgatggcaat cagttggggg
     9001 ctggagcagt tcaatcatcc gtttggtcaa ctggcgttta tcgcgaccac gctggttggg
     9061 ctgtacccaa ttgctcgtca ggcattacgg ctgatcaaat ctggcagcta cttcgccatt
     9121 gaaaccttaa tgagcgtagc cgctattggt gcgctgttta ttggcgcaac ggctgaagct
     9181 gcgatggtgt tgctgctgtt tttgattggt gaacgactgg aaggctgggc cgccagtcgc
     9241 gcgcgtcaag gggtcagcgc gttaatggcg ctgaagccag aaaccgccac gcgtctgcgt
     9301 aacggtgagc gtgaagaggt ggcaataaac agcctgcggc ctggcgatgt gattgaagtt
     9361 gcggcaggtg ggcgtttgcc tgccgacggt aaactnnnnn nnnnntttgc cagttttgat
     9421 gaaagtgccc tgaccggaga atccattcct gtggagcgcg cgacgggcga taaagttcct
     9481 gcaggcacca ccagcgtaga ccgtctggta acgctggaag tgctgtcaga accgggtgcc
     9541 agcgccattg accggattct gaaactgatt gaagaagccg aagagcgccg cgcacctatt
     9601 gagcggttta tcgaccgttt cagccgtatt tacacgccag cgattatggc cgtcgccctg
     9661 ctggtaacgc tggtgccgcc gctgctgttt gccgccagct ggcaggagtg gatttataaa
     9721 gggctgacgc tgctgctgat tggttgcccg tgtgctttag ttatctccac gcccgcggcg
     9781 attacctccg ggttggcggc agcagcgcgt cgtggggcgt tgattaaagg cggcgcggcg
     9841 ctggagcagc tgggtcgtgt tacccaggtg gcgtttgata aaaccggtac gctgaccgtc
     9901 ggtaaaccgc gcgttaccgc gattcatccg gcaacgggta ttagtgaatc tgaactgctg
     9961 acactggcgg cggcggtcga gcaaggcgcg acgcatccac tggcacaggc cattgtgcgc
    10021 gaagcacagg ttgctgaact cgccattccc accgcccaat cacagcgggc gctggtcggg
    10081 tctggcattg aagcgcaggt taacggtgag cgcgtgttga tatgcactgc cggaaaacct
    10141 cctgctgatt catttgctgg tttgattaat gaactggaaa gcgccgggca aacggtagtg
    10201 ctggtagtac gtaatgatga cgtgctgggt gtcattgcgt tgcaggatac cctgcgcgcc
    10261 gatgctgcaa ctgctatcag tgaactgaac gcgctgggtg tcaaaggggt gatcctcacc
    10321 ggcgataatc cacgcgcagc agcggcaatc gccggggaac tggggctgga atttaaagcg
    10381 ggcctcttgc cggaagataa agttaaagcg gtgaccgagc tgaatcaaca tgcgccgctg
    10441 gcgatggtcg gcgacggtat taacgacgcg ccagcgatga aagccgccgc cattgggatc
    10501 gcaatgggca gtggcacgga cgtggccctg gaaaccgcag atgcagcatt aacccataac
    10561 cacctgcgcg gcctggtgca aatgattgaa ctggcacgcg ccactcacgc caatatccgc
    10621 cagaacatca ccattgcgct ggggctgaaa gggatcttcc tcgtcaccac gctgttaggg
    10681 atgaccggac tatggctggc agtgctggca gatacgggtg cgacggtgct ggtgacggcg
    10741 aatgcgttac ggttattgcg caggaaataa agaggctatc agtctacggt ccaacaatgc
    10801 cggatgcggc gtgaacacct tatccggcct acacagagca tcgannnnnn nnnngagcat
    10861 cgaactcgta ggcatgataa gacgcgacag cgtcgcatca ggcattgtgc accgtatcgc
    10921 cagatgcagc ttgaacgcct tatccggcct acaaaccggt ttcaagcagc aacattgaga
    10981 gcgatcggtc ccctcgctcc tctggggaga gggttagggt gagggcaaaa ggcggcatcg
    11041 aagccaatct tcccccatca accgccttta cgaatcaaat aacgataagg cagtccatcc
    11101 gtctctttag caaccagttc gtgttccata aaggtacaaa acccaggaat atcacgggtc
    11161 gtcgccggat cgtcagcaat aatcagcaac gtttcacctg gctgcatatt gcgcactgtt
    11221 ttgcgcacca tcattactgg ttccgggcag cgcaggccaa gtgcgtcgag tgtgtggtca
    11281 gggctggaaa agagatcggt cattttcttc tcatcacata aaaaaacggc gctagtttac
    11341 gccctgtgag tccgtaagca aaccaggtta acgattgcgt gaaaattaac cattgcattg
    11401 tcaacgtaaa gcagtatcat gcggcggctc gaaaaaaggg tcagcacgtt attatgttaa
    11461 ggtaacagac gtgtcgtacg tattgggttc cctcacccca atggttaatc aaaaaggtac
    11521 aatatgaacg ttttctcgca aacgcaacgc tataaggcgt tgttctggtt atcgttattt
    11581 catcttctgg tgatcacctc cagtaactat ctggttcagc ttccggtctc catttttggt
    11641 tttcatacca cctggggcgc gtttagcttt ccgtttatct ttctcgctac tgacctgacc
    11701 gtgcgtattt ttggcgcgcc gctggctcgt cgcatcatct ttgcggtaat gatccccgca
    11761 ttgctgatct cctacgtcat ctcatcgctg ttttatatgg ggtcatggca aggattcggc
    11821 gcgctcgccc acttcaacct gtttgtcgcc cgtatcgcca ccgccagttt catggcctac
    11881 gcgctggggc agatcctcga cgtgcacgtt tttaaccgcc tgcgtcagag tcgtcactgg
    11941 tggctggcac cgacagcgtc cacactgttc ggtaacgtca gcgacacgct ggccttcttc
    12001 ttcattgcct tctggcgtag cccggatgcc tttatggctg aacactggat ggaaatcgcg
    12061 ctggtcgatt actgtttcaa agtgttaatc agtatcgttt tcttcctgcc aatgtatggc
    12121 gtactactca atatgctgtt gaaaagactg gcagataaat ccgaaatcaa cgctttgcag
    12181 gcgagttaaa ggttcgttat cagagttgtg ataagatgga tgaatgagcc gttatggccg
    12241 tttatcgaaa ggaagaagtc aatgcgcaat ctggttaaat atgtcggaat tggcctgctg
    12301 gttatggggc ttgcggcctg tgatgataaa gacactaacg ctacggcgca gggttcggtc
    12361 gcagaaagta acgcgaccgg gaatcccgtc aacctgcttg atggcaagtt aagtttctcg
    12421 ctgccagcgg atatgaccga ccagagcggt aagctgggaa cgcaggccaa taacatgcat
    12481 gtctggtccg acgccaccgg gcagaaagca gtcatcgtca tcatgggcga tgatccgaaa
    12541 gaagatctgg cggtgctggc gannnnnnnn nntgctggcg aagcgtctgg aagatcagca
    12601 acgtagccgc gatccgcagc tgcaagtggt aaccaataaa gccattgagc tgaaaggtca
    12661 caaaatgcag cagttagaca gcattatctc cgcgaaaggc cagacggcgt actcttccgt
    12721 tattctgggt aacgtgggta atcaactgct aaccatgcaa attacgctgc cagctgacaa
    12781 tcagcaaaaa gcacagacca ccgcagaaaa
//
LOCUS       DAWDRC010000033        12676 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2583_length_12676_cov_2.621742, whole genome shotgun sequence.
ACCESSION   DAWDRC010000033 DAWDRC010000000
VERSION     DAWDRC010000033.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12676)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12676)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2583_length_12676_cov_2.621742"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1978..1987
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10996..11005
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3148 a         3043 c         3306 g         3159 t
ORIGIN      
        1 ggcgacatcg cttccagcag ttctggtttg ccatacagca cgccgatgcc cgtcgggcca
       61 tacagtttgt gacctgaaaa agcatagaaa tcaatatcca gttgctgaac atccgcgggg
      121 aaatgcaccg ccccctgagc accatcaacc ataaccacca tcccggctga atgagcaaaa
      181 gtaatcgctc tcgccagatc cgggcaaccg ccagtgacgt tcgacatctg gcccaacgcc
      241 agaatccgac tacggggagt aatcagttct ggcaacaaat cgacatccgg cagtcgctgc
      301 gcattaagcg gcaatttcac cactttggcc cccgtttgtt gggcgaccat cagccagggg
      361 acgaggttgg cgtggtgttc tgccacactg acaataattt catcgcccgg ttgcagacgc
      421 ggacgggcat agcattgcgc caccatgttg atggattcag tggtgccgcg cgtccagacg
      481 atatttttgt catccgatgc attcagtaat tgtgccactt tttcccgcgc agcttcgtaa
      541 cgcgcggtca ggcgttgggc ttcggcaaac tggctgcgat ggacgtttcc ggcgctcagg
      601 ctataaaact gtcgggtggc ttcaaccacg gcttcaggtt taagcgcggt cgcggcgctg
      661 tcgagataga cgcccgcatc ctgtagcgcg ggaaactggg cgcgaaactg cgcgggatta
      721 aaaacgttca tggtactcct cggcttgatg caccgatcgt gacgcaaaat tcactttgat
      781 acaaggagag tgctaaccat cagaattatc tggatatcct ggcgaagccg cgtcagtaac
      841 atatgctgaa tagtgttagg cgttaatttt agcctgttaa tcaatactat taagatttat
      901 taatcgcttt aaggagaaga tgatgaaaaa gactgccgca attatttctg cctgtatgct
      961 gacttttgcc ctgagcgcct gttccggttc gaactatgtg atgcacacca atgacggacg
     1021 taccatcgtc tctgacggca aaccacagac tgataacgat accggtatga tttcgtataa
     1081 agacgctaat ggcaacaaac agcagatcaa ccgtaccgac gtgaaagaga tggtcgaact
     1141 ggatcagtaa ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg gtgctacatt
     1201 aatcactatg gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc tttgctacca
     1261 tggtctgaat cgcagaccaa ttgcaaacac aacaacacaa catcacaacc gtaagccaaa
     1321 agttcaccag aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa gtgccgccac
     1381 tataggtatt tgctggtaga agctcaacgg acaatttata atggctcaga ttaaaaaaac
     1441 taataggtta catagtgtaa tctaattgtt aaattcattt aacatcaaag tttaaaagcc
     1501 atgtctaaac gattaccacc gctaaatgcc ttacgagttt ttgatgccgc agcacgccat
     1561 ttaagtttca ctcgcgcagc agaagagctt tttgtgaccc aggccgcagt aagtcatcaa
     1621 atcaagtctc ttgaggattt tctggggcta aaactgttcc gccgccgtaa tcgttcactc
     1681 ctgctgaccg aggaagggca aagctatttc ctcgatatca aagagatatt ttcgcaatta
     1741 accgaagcga cgcgtaaact ccaggcccgt agcgctaagg gggcgttgac ggtcagttta
     1801 ctccccagtt tcgccattca ttggttggtt ccgcgacttt ccagctttaa ttcagcttat
     1861 ccgggaattg acgttcgaat ccaggcggtt gatcgtcagg aagataagct ggcggatgat
     1921 gttgatgtgg cgatatttta tggtcggggc aactggccgg ggcaaggggg ggaaaaannn
     1981 nnnnnnnccg aatatttatt gccggtgtgc tcgccgctac tgctgactgg cgaaaaaccc
     2041 ttgaagaccc cggaagatct ggctaaacat acgttattac atgatgcgtc acgccgtgac
     2101 tggcagacat atacccgaca gttggggtta aatcatatca acgttcagca agggccaatt
     2161 tttagccata gcgccatggt gctgcaagcg gctatccacg ggcagggagt ggcgctggca
     2221 aataacgtga tggcgcaatc tgaaatcgag gccggacgtc ttgtttgccc gtttaatgat
     2281 gttctggtca gtaaaaacgc tttttatctg gtttgtcatg acagccaggc agaactgggt
     2341 aaaatagccg cctttcgcca atggatcctg gcgaaagccg ctgctgaaca agaaaaattc
     2401 cgctttcgtt atgaacaata atttacgtag ggtacgacca tgaccagccg ttttatgctg
     2461 attttcgccg ccattagcgg cttcattttt gtagctctgg gcgcttttgg cgcgcatgtg
     2521 ttaagtaaaa ccatgggggc cgttgagatg ggctggatcc agaccggcct cgaataccag
     2581 gcgtttcata cgctggcgat cttaggtctg gcggtggcaa tgcagcgtcg catcagtatc
     2641 tggttttact ggagtagcgt tttcctcgcg ttaggcacgg tgctgttcag cggcagcctt
     2701 tattgcctgg cgctgtccca tctgcgtttg tgggcgtttg tcacaccggt tggcggcgtg
     2761 agcttccttg cgggctgggc gttaatgtta gttggtgcta tccgtttaaa gcgcaagggc
     2821 gtaagtcatg aataaggttg tattgctgtg ccgtccgggc tttgaaaaag agtgcgccgc
     2881 agaaattacc gataaagccg gccagcggga aattttcggt tttgcccgcg tgaaagagaa
     2941 tgcgggttat gtgatttatg aatgttatca acctgatgat ggcgataagt taatccgtga
     3001 gctgccgttc agttcattaa tttttgcccg ccagtggttt gtagtggggg aactcctgca
     3061 gcatttgccg ccagaagatc gtattacccc cattgttggc atgttacagg gcgtagtaga
     3121 gaagggcggt gaactgcgtg ttgaagttgc cgataccaac gaaagcaaag agttgctgaa
     3181 attctgccgt aaattcaccg tgccgctacg cgctgccttg cgtgatgcgg gggtgctggc
     3241 gaactatgaa acgccgaagc gtccggttgt gcatgtattc tttattgcac caggctgttg
     3301 ctatactggt tactcataca gcagcaataa ttcgccgttc tatatgggca ttccgcgcct
     3361 gaaatttccg gcagatgcgc cgagtcgttc cacgctcaaa ctggaagagg catttcatgt
     3421 gtttattccc gctgatgagt gggatgaacg cctggcgaac gggatgtggg cggtggattt
     3481 gggcgcttgc ccaggcggct ggacctacca attggtgaag cgcaacatgt gggtttattc
     3541 cgtcgacaac ggcccgatgg cgcaaagtct gatggatacc gggcaggtga cgtggctgcg
     3601 ggaagacggt ttcaaattcc gtccgacacg cagcaatatc tcctggatgg tatgcgatat
     3661 ggttgaaaaa ccggcgaaag ttgcggcatt aatggcgcag tggctggtta atggctggtg
     3721 ccgtgaaact atcttcaatc tcaaactgcc gatgaaaaaa cgctacgaag aagtgtcaca
     3781 caatctggcg tatattcagg cacagcttga tgaacatggc ataaatgctc agattcaggc
     3841 gcggcagttg tatcacgatc gcgaagaagt gacggtgcac gtccgccgta tctgggctgc
     3901 ggtgggtggt cgtcgcgacg agcgataaca ggcaaggccg gggcgtcgtc tccggcctgc
     3961 taccctttct gtcaggctgt taccaaagaa gttgcaacct ggcgctcatc agaaataatg
     4021 tgcgttacct catccagatg cccgatgcgg gcttcccctg aacgattaaa tttactttta
     4081 tcaatcaata acaacgattg cgcggcacgt tttaataaca ttgatttgta atcagcgttg
     4141 atggcattgg agtcccacag tgcgccgctg ctatcgatcc cttcacaaga aaaaataaac
     4201 agatcgattt ccagcgattt aagctgggaa atcagcgagg gattgacgta acaaccatat
     4261 ttgcgctcaa gtgtgccgcc ggaactgatc agttgaatgc gttcacgttt accgagttca
     4321 tggcaaatcg gatggctatt ggtgaagacc tggatgttga tgtcaggcaa ctggcgtgcc
     4381 agataccagc aggttgaact ggcgtctaat gctattacca tcccttcttc aatccacgcg
     4441 agcgcctcgc gcgcgatatc tgctttatgc gcataatggc ttttcaaccg gatgtgaaag
     4501 ggatcgccac tgtcttgatt ttgacggtgg atatatttag cgcgtccatg attgcgcaga
     4561 attttaccct gcgtctgtaa ttcattgaga tcgcgacgaa tggtttcttt actgaccttt
     4621 agctgttcag agagagcttc cgtggtcagg ctggtatggt tcagcagcag gtcgactatc
     4681 gcttgctggc gtgccgcttt catatttttc accccatcat tcgataaccg cccgcgtcat
     4741 gcgggcgtac cggtatgaga ttacggtgtt accccttttt ttaaaagaat attcccgtac
     4801 ttcgctcgtt cgcctgtgat aacgatcgca aaggcttttt gcgcccgttc ataaaacgca
     4861 aaacgattga tgcggatgat gtcaggacag ggggcttgta gtgaaagcgc attacggtaa
     4921 cgtcgttcta cttcaggatc gagagtgtca ccttctaccg ccgccatcat caccagcggt
     4981 ggtgcataac tgtccagctc aaataacggg ataatcgcct ggagcaagtc gctcaccaac
     5041 aggccatcag cgcggatcac ctgcggtccc atcgaatggg cgggaaagtg agcatcggaa
     5101 aaaataattt catctccatg ccccatctct gccagcactt tcaatagttc gggagaaatt
     5161 aacggcgaaa ttgttttcag catttcacac ctcctctata aattcaggtt cagtttgcgg
     5221 gtagaaataa cggtactgat aatgaatctg tgcacgggct tcttccgggc tgttaaattc
     5281 ccctacgcca taccaaccaa acagcgcagc tcctgcgacg gtcgtttcgg catcgtcgag
     5341 aacttttacc ggaatatcaa gcatattggc tttaatctga ttccacaatg tgttgcgact
     5401 tcctccgccg actaacaata attcagaggc tttaaagtgc ccgatttttt ccagcatctg
     5461 tagattgcgc tgtaattgcg tggttaaccc ttccagcgcc gcgcgataga aatgcccccg
     5521 cgtggtatta agcgttacgc cttgccagcc agcgttctgg cacgacaata aatcacactg
     5581 cattttcact ccatctgcgc caggcgcgat caggcgagct tcttcaatca atatttgcca
     5641 gggagtttca gccgtccaga acagttttct cacccattcc agcacgccgg atgccagcca
     5701 ttgcatacct gggttataca accctgcctg gctatccagt tcgcaggtag aaccggcgta
     5761 ctgacttaac agcgaagtat caacctgggc gctgcggacc attaaaattt cccatgtacc
     5821 ggaagagagc acgggttcat tttgttcggc accagcgcca aaaagggcga actgggtatc
     5881 gtgacctgcg gaaatcaccg gtatgccaac gggtaagccg agcattgctg cggcgctgtt
     5941 ctgtagcgta ccaatctgtt cacccgcttc caccagacga gggaagagtc ggcgtggaat
     6001 accggtggct tgtaaaattt gcggactgaa atcgcgttgc tggatatcca gcatctggct
     6061 ggttccggcc atcgtaatat cagtagtgaa ttcgccggtt aaacggtggt taatcagcga
     6121 cgaaataaag agccaggcgt gcgcgcgttc cagcagttgt ggatgatttt ctttcaacca
     6181 caccaactta tataacgtat tgaaactaaa ggctccgact ccagaaatag cctgcaactg
     6241 ctgtgcggag atcaaccgtt caatatggtc cattaccgcg gctgttcgcg gacatttcca
     6301 gctaatgatc ggatagagca gattgccttg cttatctacc agagcgccat ccacaccaaa
     6361 ggtggtgacg gcgataccgc ggatgtggca atcagtcagt tcactattga tttgccgaca
     6421 gcaatcagca aagcgttgca aaatggcgtc taaagaccac tggtgccagg tgttgttttc
     6481 catcgcgata tcgctggcat taggcgttga ggcgcgggca acaatttttc cctgccgatt
     6541 aaccgcgatg gccctgacat tggtcgcgcc acagtcgagt accaggataa cttcttgttt
     6601 cataatcgct cctgcaatat agccggataa cattgcttat ccggctaacc actcttgctt
     6661 gttcaggccc ggtagccgga gcgaccgggc atcacatcag ggagtaatgt attaacgctt
     6721 gtacaacggg ccgtagttct ggcaagcgcg gtaatcctgg ccttcaatat ccatgccgtg
     6781 cgcggcccag gcagaaggac gatacacttt ggtctcttca acgttgtgca tacataccgg
     6841 gatacgcagc atggaggcaa gagtgataaa gtctgcgcca acgtggccga tggtcagaac
     6901 cccgtggtta gcgccccagt tcgccattac cgagtacaca tccgtaaacg gccctttacc
     6961 ggtgaggcgc ggtgcaaacc aggtggttgg ccaggttgag ttggtgcgtt tgttgaggat
     7021 gtcatgcaca tccttcggca attccacgct ccagccttcc gcgatttgca gtaccggtcc
     7081 caggcctttg atgatgttga cacgagtcat ggtgaacggg acgccgcctt cggtaagaaa
     7141 gcgggaagag taaccgccgc cacggaagta ttcgtggatc gccgggcacc attcggtagc
     7201 ggcgaggcaa gcgtcagcct cttgctgaga gatttcccag tgcggcttca tcgtcggttt
     7261 accttcgctg tcgcgttgtt tacaggaacc gtccagcgca gcagaaccgg agttgatcaa
     7321 atggatgatg ccgtgttctg ccagcccgtc cagtttatgc cccgttacac gctcaattgc
     7381 ttctggtgac cagtaggtac ggacatcggc aaatacctga gcggtgccgg tgagctggtg
     7441 gcccattaac attgccacgc cgttaagact gtcgttttcg gtcgccacga caaacggttt
     7501 gcgcacgcca ttccagtcaa atgaactgtt gaggatcgct tcagcggtat cgccattggg
     7561 atattgatcg gtccagtgac gttgcccctg gaagcctgca gcgatggcgt tgtagccaag
     7621 tgattcttcc acgcgaccaa tatcggccag tttgctgttg ccttgcatca tgtcgcggat
     7681 acacatcgcc atcagtaaac tttcgcgcag aactgcgcgg ctttgctcgg cattgcgttg
     7741 atactgtttg ttattttcat cttcgccata gcggaagttt ttatcagccc aggccagcgc
     7801 catttccagt tcggcttcgt cgtaaatctt ctgatcgata cggcgacgca gttcggtcat
     7861 atccaccgcc tggactttca ttcccagcca ggattcaaag aagttgtgat caacgatgga
     7921 accggcgata cccatcgaaa cgccgcccag cgacagatag cttttacctt tcatgctggc
     7981 gactgctaaa ccggcgcggg caaagcgcag cagtttttct tcaacatcgg caggaatcga
     8041 tgtgtcatcg gcatcctgaa cgtcatgacc gtaaatggag aatgccggga tgcctttctg
     8101 gctgtgagct gccagagccg ctgccaggta aacagcgccg ggacgttcag tgccgttaaa
     8161 gccccaaatg gctttcgggc gggttggatc catatcgatg gtttcactgc catagcacca
     8221 gcaaggggtt accgtaatgg tgaggcctac attctgactg ctgaattttt cttcgcaagc
     8281 agcggcttca gccatacccg cgatacaggt atcggaaatg acacactcga cggcagctcc
     8341 acaggcatgg cgcagtttct cggtcagcag tgcagccgtg gctttcgcca tattcattgt
     8401 ttgttcttca agcgactcac gaacacccat gcgacgaccg tcaataaccg ggcggatacc
     8461 aattttcggt aagctgattt ttttcattac tgattcctca ctttattcgg aaaatatgtt
     8521 cagttagttg ccgtttgaga acggaaacgg gcaaagataa agatgactgc gaagcagagt
     8581 gccgggatca gttcggcagt ggggatgttg cccgctgcgt cactgacaaa ccccatgacc
     8641 ggagtgacaa taccgccgcc aataatggtc ataacgatga aggacgaacc gtatttggtg
     8701 tcctggccga gattcttaat gcccagcgag aagattgttg ggtactgaat cgacataaag
     8761 gcactgcata aagtcagggc tattaaaccc acatgaccac cagcgaaggc tgagatcagg
     8821 cacagtgcca tagcgattaa tgcgtaggct gccaggactt tgtgtggtgc gaagcgactg
     8881 atgagccagg taccggtgaa acgaccaata aagaagcaca ccatggttcc ggttaaatag
     8941 ttagcggcaa agcctgctgt catacctgga atttcttcta cagcgtagcg aattaaatag
     9001 ctccagcagg ccgtttgtgc gccaacgtag cagaattgcg ctaatactgc ccagcgccag
     9061 tggcgaatac gcgccaggcg agaaagcgat gcggagaacg atccttgttt ggcgtcactg
     9121 tgattatcac tctgcaatgc cgggaatttc gtcagcatga tcagcagggc gaccagtaac
     9181 acgatagcca cgatgatcat ataaggtgtc tgtaccgata ataccaggct gtgtttatac
     9241 gcactcaatt gctctggaga cattttatcg agaacgtctt gcgattgatg tggcacgtta
     9301 gacaaaataa gactttgccc aaagacaacc gcgataattg cgccaaatga gttaaatgtt
     9361 tgcgcaagat ttaagcggaa gtgaccactg ctttccggcc ctaataccgt aacaaaaggg
     9421 tttgcggcag tttccagaca acctaatccg gctgcaataa taaataggcc aactaaaaac
     9481 aaggtgtagt tcattatttc tgcggcgggc cagaataatg cagcgccgaa ggcatataaa
     9541 aacaatccgg taataatccc tgctttataa ctgagttttt tcatcaatat cccggcaggg
     9601 attgggataa tgaaataacc aaagtaaaag gccgattgga tcaggccagc ctggaaattt
     9661 gtcagcgtaa aagcctgctg gaattgaggc aataaaatgt cgttaaggtt attggctacc
     9721 gcccaaagaa aaaacagtga gcacagcagt gcgaatggaa taatgtaact tctgctctgc
     9781 cctgcatctt tatctaccgc acggtaactc tgcgtttgta ttgatgtgtt tcccatagca
     9841 tcctcttagg ttcagaagct taagcacgta atcggttatg ttttcaaaag tgcaaaacca
     9901 gcgagttcgg gcttcatatc agtgaatatt tgcctgtctg aaatgaactc atggattcat
     9961 tttcaaataa aaaacggtct gtaatatgac ggcggtcaca cttattagct ggaaggtgac
    10021 tttatgtgac taccatcact ttaataatcg aaatttcatg cccgtttcag aaaataaatc
    10081 ataaaaaaca caaaccagat gaccgtatta attgtttgtg ccgaaaacgg gcattcctgg
    10141 cagggatgag tagaaaaatg accgttttga taaatatttt taagagcggt catttttaat
    10201 gtggttataa aaagagaaat tgcagttatt tactgtgatt agttgaacca ggtcacaaaa
    10261 tataagcttg tgaatttctt tctggttaat caggaagaat atggtgagta attaaacggc
    10321 taattcaata gtgtgaaagg aacaacatta ttgccctgtt ttgaatcaga gagaggtagc
    10381 taatggaacg aaataaactt gctcgtcaga ttattgacac ttgcctggaa atgactcgcc
    10441 tggggctgaa ccaggggaca gcgggtaacg tcagtgtacg ttatcaggat gggatgctga
    10501 ttacgcctac aggtattcca tatgaaaaac tgacggagtc gcatattgtc tttattgatg
    10561 gcaacggcaa acatgaggaa ggaaaactcc cctcaagcga gtggcgtttc catatggcgg
    10621 cctatcaaag cagaccggat gccaacgcgg ttgttcacaa tcatgccgtt cattgtacgg
    10681 cagtttccat tcttaaccga ccgatccccg ctattcacta catgattgcg gcggctggcg
    10741 gtaattctat tccttgcgcg ccttatgcga cctttggaac acgcgaactt tctgaacatg
    10801 ttgcgctggc tctcaaaaat cgtaaggcaa ctttgttaca acatcatggg cttatcgctt
    10861 gtgaggcgaa tctggagaaa gcgttatggc tggcgcatga agttgaagtg ctggcacaac
    10921 tttacctgac gaccctggcg attacggacc cggtgccagt gctgagcgat gaagagattg
    10981 ccgtagtgct ggagannnnn nnnnnacggg ttacggattg aagagtaatt tcgtaaagca
    11041 acaaggagaa ggatgatggc taacagaatg attctgaacg aaacggcatg gtttggtcga
    11101 ggtgctgttg gagctttaac cgatgaagtg aaacgccgtg gttatcagaa ggcgctgatc
    11161 gtcaccgata aaacgctggt gcaatgcggc gtggtggcga aagtgatcga taagatggat
    11221 gcggcaggac tggcatgggc gatttacgac ggcgtagtac ctaacccaac aattactgtc
    11281 gtcaaagaag ggctcgatgt attccagaat agcggcgcgg attacctgat cgctattggt
    11341 ggtggttctc cacaggatac gtgtaaagcg attggcatta tcagcaacaa cccggagttt
    11401 gccgatgtgc gtagcttgga agggctttcc ccgaccaata aacccagtgt accgattctg
    11461 gcaatcccca ccacagcagg cactgcggca gaagtgacca ttaactacgt gatcactgac
    11521 gaagagaaac ggcgcaagtt tgtttgcgtt gatccgcatg atatcccgca ggtggcgttt
    11581 attgacgctg acatgatgga tggtatgcct ccagcgctga aagccgcgac gggtgtcgat
    11641 gcgctcactc atgctattga ggggtatatt acccgtggcg cgtgggcgct aaccgatgcg
    11701 ctgcacatta aagcgattga aatcattgct ggggcgctgc gaggatcggt tgctggtgat
    11761 aaggatgccg gagaagaaat ggcgctcggg cagtatgttg cgggtatggg attctcgaat
    11821 gttgggttag ggttggtgca tggtatggcg catccactgg gcgcgtttta caacacgcca
    11881 cacggtgttg cgaacgccat cctgttaccg catgtcatgc gctataacgc tgactttacc
    11941 ggtgagaagt accgcgatat cgcgcgcgtt atgggcgtga aagtggaagg tatgagcctg
    12001 gaagaggcgc gtaatgctgc tgttgaagcg gtgtttgctc tcaaccgtga tgtcggtatt
    12061 cctccacatt tacgtgatgt tggtgtacgt aaggaagaca ttccggcact ggcgcaggcg
    12121 gcactggatg atgtttgtac cggtggcaac ccgcgtgaag caacgcttga ggatattgta
    12181 gagctttacc ataccgcctg gtaattgcgc ggatgtgata atgccggatg cggcgctggc
    12241 gcgtcttatc cggcctacaa gtgcattaaa tttaatggat tgttaatcgg tagcaccctg
    12301 tatctcttta tcgtaccaac cgcaattgct gtaaattgcc gtcgatatgc atatcggttt
    12361 gtaagcgggc aatatcgcgg cacagaaacg ccatctcttt atgggtttct aattttttgc
    12421 gccacttttc ggcaaccgca tccagattct catatatccc ttccagactc tgaaactcga
    12481 ccagtagctg tgtggcgctt tttggtccga tccccgcaac acccggtacc tttgaactgc
    12541 tgatccccgc aagcccccag taatcgggca actgctgcgg ttgaacgcca aattctttat
    12601 cgataaatgg cgcatccagc caacgtttct ggaagtaatc acgaatacgt aatgtcggtg
    12661 aaagtaactg acagta
//
LOCUS       DAWDRC010000034        12589 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2601_length_12589_cov_2.381682, whole genome shotgun sequence.
ACCESSION   DAWDRC010000034 DAWDRC010000000
VERSION     DAWDRC010000034.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12589)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12589)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_12589_cov_2.381682"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8433..8442
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3066 a         3205 c         3228 g         3080 t
ORIGIN      
        1 actgctggta gttgcggcgc tggtcgttct gctgtttggg actaagaagt tacgtacgct
       61 gggcggagac cttggagcgg ccattaaagg gttcaagaag gcgatgaatg atgacgatgc
      121 tgcggcgaaa aaaggcgcag acgttgatct tcaggctgaa aagctctctc ataaagagtg
      181 acgtggcgag caggacgctc cctcaatatc ttgttcgata caaaaacccg cttcaaaaag
      241 cgggtttttt atcagacaga tgtaagtaat tattacagga ttacttaact tccatccctt
      301 tcgcctgcaa atcggcgtgg taagaagagc ggacaaacgg accgcatgca gcatgggtaa
      361 agcccatcgc cagcgcttcg gctttcattt cgtcgaactc atccgggcta acgtaacgtt
      421 gaaccggcag gtgatggcgg cttggctgca aatattgccc cagcgttagc atcgtcacac
      481 catgacggcg caggtcgcgc attacctcaa taatttcttc attggtttca cccagtccca
      541 ccatcagacc agacttggtc gggatttccg gatgcgcttc tttaaagcgt tccagcagct
      601 tcagcgacca gttgtaatct gcaccaggcc gtacctgacg gtaaatacgc ggtacgtttt
      661 ccaggttatg attgaacaca tctggtggtg tggcggtcag aatatccaga gcacgatcca
      721 tacgaccgcg gaaatccggc accagagttt caattttgat ttgcggcctt ttttcccgaa
      781 tggcagtaat gcaatccgca aagtgctggg caccgccatc gcgcaggtca tcacggtcaa
      841 cggaggtgat aaccacataa cgcagcgcca tatcggcaat ggtctgcgcc agtttcactg
      901 gttcattggc atcaggagct accgggcgac cgtgggcaac gtcacagaac gggcaacggc
      961 gggtacaaat agcgccgagg atcataaacg ttgctgtgcc gtggttaaag cattccgcca
     1021 ggttagggca ggaggcttcc tcgcagacag aatgcaggcc atttttgcgc attgcggctt
     1081 tgatgccctg gatacgtgta gagtccgctg gaagcttgat tttcatccat tccggcttgc
     1141 gcagcagggc ttcgcgctct gttgccacgt ttttaaccgg gataagggcc atcttatcgg
     1201 catcgcggta tttaacaccg cgttccatca caatgggttt actcatagcg tgcgtgttcc
     1261 agttgcgaat tacgaaggaa agcgtttcaa ttcaatagat tgttgtaatt atcaactatt
     1321 tttgaattaa cgactggcag tatatcattg aaacggacct gaaagcagcc aaagcggtcg
     1381 cgaaaatgta aaattgttgt tggattgtgc cattttatcg ttctgcgctg tgatctggct
     1441 gtaacaatac tttttcaaaa tgcattacgg ataacatcta tggtcctgtg caaaacatgt
     1501 tatcggtgat gagatcggtt tgaaactgac tgagttggtt ttccgcgccg ccgatgggcg
     1561 ggttgcgcag taaaagctgt ggatagtgag ttttaatcgc ccgatagatg acaggaagga
     1621 ccagggctcc gaccgagggg ggcagattaa gcactttcgc tgcattaacg attcctttat
     1681 ggacatatgc aacttcaaaa tagtcagaag agtaagatca atatttcgta aggttgtaaa
     1741 tatttgtggt ttaccttctg acgattttct acttaaacag ggttcaattt gattattact
     1801 atccacgcac ttactcaaat tttattcatg gaaaaataat atttataaaa attacaataa
     1861 tcttatgtct aattgggacg gaacgctttt gctcaccatg gtcaattatt tcaatgagtt
     1921 aatcaattag gtgggctacg gagcaaggcg tagcatcgca tttctgcaag tgataaagaa
     1981 aatatacaaa cgcgaaggag atgtaaagca ttagcaggta ttatgagtaa tgacccagtg
     2041 taaattgggc cattgatgta tggaattaag cggtaatata ttcgaagtca ggattgttta
     2101 gtagcgctaa aatattttcc agtaaacgtg gtgcaatatt attagttgtc gcttcgggtt
     2161 tccattgtga tattttagcc atttccattc ctgcataccc acaaggatta atacgtaaaa
     2221 atggtgaaag atccatattg acgtttaatg ccagaccgtg gaatgaacaa ccgcgtcgaa
     2281 tacgtaaacc cagtgagcaa attttctttt ccccgacata gacaccaggc gcgtcagccc
     2341 gaggatgcgc ttctataccc agttcagcca gggtattcac cactgtttgc tcaagcaagg
     2401 tcaccagttc acgcacaccg agtttacggc gtttcaggtt aagcaacaca tacatcacct
     2461 gttgccccgg cccgtgataa gtcacctgcc caccgcgatc gctctggatc accggaatat
     2521 cacccggcat taaaatgtgc tccgcctttc ctgcctgccc ttgggtgaat accggatagt
     2581 gctcgaccag ccagatttca tcaagggtac tatcatcgcg ggtatcggtg aattcatgca
     2641 tagcctggga gattggctcg taaggctgaa gaccgagctg gcggacaaga attttatcct
     2701 gatacaaaac ggcgtctccg tggagaatga gtaaaagtgg gggaaaagta tatcacagcg
     2761 aggagagggg agttacccga ccaggagccg ggtaacggag aagcgagtta cagaaccatg
     2821 cggacaatat cgattttgcc cagttcttca tacagtgttt caacctgctc gatatgagtg
     2881 gcgttgatag tgatagatac cgagtggtag ttgcctttgc tgcttggttt taccgttggg
     2941 gtgtagtcac ctggcgcatg gcgctgtacc acttcaacca cctgatcaac cagctcaggt
     3001 aacgcctgcc ccataacttt gtaagtaaaa ggagtaggga attcaagcag ttcgttaagt
     3061 ttggttttca tgtcagctcc ggcgtaacgt aattaaatag caactcccgc cagaaggcgg
     3121 gagtttttta ctgatgctta gtatatgggg acggaaatta cactttcaag tgttcaattt
     3181 ttaaccaaac cagtgatgga acattaattt aatgtaatca atgattttgc cgaagaagtt
     3241 accttccggg atttcttgca gcacaaccag cgggcgttgt tcgatcgttt tgccatcaag
     3301 ctggaagttg atagttccga cgacctgatt cttttgcagc ggcgcatgca attcactgct
     3361 gttcagcaca tagctggctt tcagatcttt catgcgacca cgcggaatgg tcaggtacac
     3421 gtctttatca acccctaacg aagcgcgatc agaatcacca aaccaaaccg gttcagaggc
     3481 gaactcttta cctactttca gtgggttaac ggtttcgaag aaacggaagc cccaggttag
     3541 taatttttta ctttcggctt cacggccttt aaaagtacgt ccgcccatca ccgcagagat
     3601 caagcgcatc tggccttcag tcgcagaagc aacaaggttg taacctgctt tgtcagtgtg
     3661 tccggttttg atgccgtcga cattcaggct gttatcccat aacaggccgt tacggtttag
     3721 ctggcgaata ccgttaaacg taaattcttt ttctttatag atcgagtatt cattcggtac
     3781 gtcacggatc aacgcctggc cgatcagcgc catatctcgc gcggagctgt actgaccatc
     3841 agcatccaga ccgtgtaccg tctggaagtg ggtatttttc aggcccaggg cgttaacgta
     3901 gctgttcatc aagccaacaa aagcgtcctg gctaccagcg gcaaaatcgg ccatggcgac
     3961 acaagcatcg ttacccgatt gcaggttaat accgcggatc agctgagaaa ccggaacctg
     4021 catgcccggt ttgaggaaca tcagcgaaga acctttaaac accgggttac cggtggccca
     4081 tgcgtcgttg ccgatagtga ctaaatcagt ttctttaaat ttaccggctt tcattgcctg
     4141 gccgataacg taactggtca tcattttggt caggctggca ggatcgcggc ggacatcagc
     4201 gttctgttcg gcgagtactt tgccggagtt atagtcaatc aggatgtagg actccgcgtc
     4261 gatctgcggt acacccggga tcatggtttt gatattcagg tcatcggcat gtgcagcaga
     4321 gataaaggct gtgcaaagag ccgtggtgag cgccaggcgc ttcatgatac gagcggaaaa
     4381 aatggtattc atggtctgaa ctacgacatc cgtgatggaa ttaaaaaaag tgccctacta
     4441 tagcaaatgc actaccggca ggcatctgac tttccgcgtg actttgttaa cgtcatttac
     4501 agaaattgac acatcagatg cctgcttcac gctactgcgc ggtggtaata aatgactgta
     4561 attgggcttc ggtttgcaaa cgttgctgca aggtactggc ttcggctttg ctggcgaatg
     4621 ggccaagctg gatccgccag accgcgccat tttgagttac gcgaccgggg acgccgaact
     4681 tctgtcccag ttgctgttgg tactgttgcg cacgagcctg atcgctaacg gccccgactt
     4741 gcaccataaa gttgccgctg gcgctttgcg aggcggcttg cggtgtcacc attgcaggtg
     4801 aggttgcagg cgttgtcgac ggagctgtaa caacgggctg tggcgcaggc gtcggttcgc
     4861 tgccttccag tacaccaggc gctaaggtcg ttggtgcgcc gaggaaaccg ctgctggtta
     4921 ccggcgcgcc ggtcggatct tcgcttttta gcgtcgaatt actgaccgga agaatgtcac
     4981 cctgcgggac aggcactgaa cttgttcccg cgccaccgct taaatcggga ggtgctggca
     5041 gggcgtaagt ctgtttggcg actgtggtac aagccatacc aggaccagaa agcgaaccat
     5101 cctgggcgac aataatcgga tcgatacgaa ctttggtgtt gtttgacgtg ttaagacggt
     5161 cagctgccgc gcgagaaagc gaaatcacgc ggtcgttgcc ataagggccg cgatcattaa
     5221 tgcgcaccac gatcattcgc ccgttagcca ggttagtgat tctggcgtag ctggggattg
     5281 gaagcgttgg atgggccgcc gtcaactgtg tcggatcgaa agcttcgcca gaggccgtca
     5341 ggttactgcc gggttcggca tcatagattg ccgccagtcc cgcctggcta aatcgagacg
     5401 gatcctgcac gattttgtag cttttcccgt cgcgctggta atcctgattt gccgtcgcgt
     5461 tcagtggttc gaaacgcggg tctgccccgc taatttcaac tatggggccg ttacataccg
     5521 caggctgcgg tacacttacc gtctgttgct gaccatcatc gcttgtacat gccgcgagca
     5581 ttcctgccgc gatgcagatc ccgagccact gcttacgcat tgcgcacctc ttacacgctt
     5641 ttcgacaaca ttttcctgtg ggtgtggatt gacattacaa tcccgaaccc agccatcagc
     5701 acaattagcg ccgatcctcc ataactgacc agtgggagcg gaacccctac aaccggcaga
     5761 ataccgctta ccataccaat atttacgaag acataaacga ataatatcag cattaagccg
     5821 ccagccatga cgcgaccaaa ggtggtttgc gctctggcgg ctatccacag cccgcgcatg
     5881 atcagcagaa tgtagagagc gagcagaatc aaaatgccca ctaatcccag ctcttccgcc
     5941 agtaccgcga agataaagtc ggtatggcgt tcggggagaa attcaagctg tgactgagtg
     6001 ccgtgcagcc agcctttgcc gcgtaatccg ccggagccaa tagcaatttt agactgaata
     6061 atgtgatagc ctgcgccgag tgggtctgat tccgggtcca ggagcatcat tacgcgctgg
     6121 cgctggtaat catgcatcag aaagaaccac agaatcggaa tgaacgccgc taccagcact
     6181 actgcgacgc caatcagacg ccagctaagg ccagagagga acagtacaaa caggccggaa
     6241 agcgcaacga ggattgatgt tcccaagtca ggctgtgcag ccaccagcag cgtgggcata
     6301 aatatcagca ccagcgcgat agctgtattc ttcaatgaag gtgggcaaac gtcgcggttg
     6361 ataaagcgtg caaccatcaa cggtacggct atcttggcaa tttccgacgg ttgaaaacga
     6421 acaataccga ggtccagcca gcgttgagcg cctttagaga tggcaccgaa agcatctacc
     6481 gccaccagca aaataataca gatgatatag agataggggg cccagccttc ataaacgcgt
     6541 ggaggaattt gcgccatcac caccatgatg accagaccca tcgcaatctg gccgatttta
     6601 cgctccatca tgccaatatc ctgaccgctg gcgctccaga taaccagggc gctgtaaacc
     6661 agcaatgcca gtaagatcag cagcattgtg ggatcgagat ggactttatc ccagaatgtt
     6721 tttttattcg gattatccgt catgattaat ggtcctccgc tgcggcaacc gctggatttt
     6781 ccgcaggcag atcggtgttg ttatcaccca gcataatgtg gtcgaggatc tggcgcatca
     6841 gcgtaccaac cgccggaccc gcgccaccgt tctccagaat catggcgaca gccacttgcg
     6901 gattgttgta tggcgcaaag gcggtcatca gtttgtggtc acgtaaacgc tcggcaattt
     6961 tgtgcgcatt ataggtttcg ttcgctttca gaccgaagac ctgagcggta ccggatttcg
     7021 ccgcaatttt gtacggtgcg ctggcaaaat atttatgcgc cgtaccgtta gggcggttag
     7081 caacaccgta cataccgtct ttcgccagct cccagtaacc ggaatgaata tcgccgacgg
     7141 gcggttcatg cggttgtacc catggcacct gtttgccgtc ttcggcggtg ctcatcagta
     7201 aatgcggaac cttcacgata ccgtcattaa tcaggatcat cagtgcctta ctcatctgga
     7261 taggggtcgc cgtccagtaa ccctgaccga taccaaccgg aatggtgtca ccctgatacc
     7321 acggtttttt aaagcgtttc tgtttccatt cgcgggtagg catgttgccg gaacgttctt
     7381 ccgccaggtc gataccggtg taatgaccat aaccgaattt ccccatccat tcggagaggc
     7441 gatcgatccc catatcgtag gccacctgat agaagaaggt atccgcagat tcttccagcg
     7501 atcttgtgac attcagacgc ccgtggcccc attttttcca gtcacgataa cgtttttccg
     7561 accctggcag ttgccaccag ccggggtcaa acagcgtcgt attgcgcgtg atcaccccgg
     7621 cactcaatgc cgaaaccgcc acatagggtt taactgtaga cgcgggagga taaaccccct
     7681 gtgtggcgcg gttcaccagc ggtgtattcg ggtcgttcag caaggcggaa taatctttgc
     7741 tggagatacc gtcaacaaac aggtttgggt cataactagg cgtggaaacc agcgccaaca
     7801 ccccaccggt acgcggatcg gtgactacca cagctgcgcg gctacccgcc agcagcgttt
     7861 caatatattg ctggagtttg agatccagcg tcaggtaaat atcgtgtccg gcttgcggtg
     7921 gcacttcttt taactggcgg ataacacgcc cacggttgtt aacttcaacc tcttcataac
     7981 cggtctgacc gtgcagcacg tcttcatagt aacgttcaat gcccagctta ccgatatcat
     8041 gcgttgccgc atagttggcc agtttgccgt cattattcag gcgttcgacg tctttatcgt
     8101 tgattttcga cacatagccg atgacgtggg tcaacgccga accgtaagga tagtaacgac
     8161 gtttatagcc tttaacttca acacccggaa aacggtactg gttgacggca aagcgagcta
     8221 cttgtacttc ggtcaggtta gttttcaccg gaatagaggt gaaacggtgt gaacgtgcgc
     8281 gctcttttcg gaatgccgca atatcatcat cggtcagatc aaccacgctg cgcaaagcgt
     8341 ccagcgtttg ctgcacgtta tcgactttct ccggcatcat ttctatctgg tagatagtgc
     8401 ggttgagagc cagagggata ccgttacggt cgnnnnnnnn nnattataca ggttggcgat
     8461 aagcacgccg gtcagcagca aaatccccaa aaaggcgacc agcgcccggc gcacaaacag
     8521 cgcggactct gccgtatagt cgcgaaaaga gttctgtagt ttcatccgct gcgttttcta
     8581 ctcaaagctc acttatcact cacggtgata aggatggttg gtggtgatgc tccacgcccg
     8641 gtacagactc tctgcgacca gcacgcgaac cagcggatgg ggaagggtaa gtgccgacag
     8701 cgaccagctc tgctccgccg ccgctttaca ggcaggcgac aacccttcag gcccgccaat
     8761 cagtagactg acgtcgcgac catccagctt ccagcgttcc agctcagcgg ctaactgcgg
     8821 cgtatcccag ggcttgcctg gaatatcgag ggtgacaatg cggtttttgc ctgcggccgc
     8881 caacatctgc tcaccctctt tgtcgagtat gcgcttgatg tccgcattct tgccgcgttt
     8941 tccggccgga atttcaatca gctcgaaggg catatctttc ggaaaacgac gcaggtactc
     9001 ggtaaaaccg gtttgtaccc agtccggcat tttcgttccc acggcgacaa gttgcagctt
     9061 cacgcattaa ctccagagtt tttccagttc atacaggcga cggctctctt cctgcatgac
     9121 atggacaatc acatcgccca aatccacgac aatccagtcg gcgctgtttt caccttctac
     9181 gccgagcggt aacaggcccg ctgcgcgaga ctcctgcaca acgtgatcag caatggacat
     9241 aacatggcgg ctggacgtac ccgtacagat gatcatgcag tcggtgatgc tggatttgcc
     9301 ctgaacgtct aaggcgatga tgtcctgacc tttgaggtca tcaattttgt cgataacaaa
     9361 atcctggagt gctttaccct gcaagttttc cccctgggtg aatcaaatag ataaaaatgg
     9421 tctgtcagta tacctgaacc agaggcgatt tcgggacaat tgtcgccgaa tcggctttcg
     9481 aaagtgggct atcatcccac cccgcgccgc agattgcatc gccatttttg taaaacaatt
     9541 tctacaaagt cgtgtctggc ggaaaaagtc tggctgcgga gaatatcagc ctgcccgggg
     9601 ctgtcaatgg tcgttgcggc ttacccgtaa aaaaacagaa aagtcatgca ttctcgaccc
     9661 cgatggcacg gctattgagg acgcgtagcg tcgcgaattt ttggttgata tcaatggcgc
     9721 tccaacaccc ctggtcaacg cggaaatgcc acatagactc ggcaggcata ccaattaaac
     9781 gagtaattaa cagactcagt acgccctgat ggctgacgac taaaatattc tgatagtgct
     9841 gatattcact aagccttgcg ataaatcgtt ccacacgttg cgaaaatgcc tgaaatcctt
     9901 caccgttcgt ggggatagca tgctgccagt cattgcacca cgcgctatag ttttcggcat
     9961 cttcttgcat gaggtcacga tgatgtcgca tctcccagtc gccaaaaaac atttcattga
    10021 gttcagggat gatatgaacg gggtgctggc ggtcactaag aaccagtcgc gcggtatgct
    10081 gtgcccgttc cagttcactg cataaaacca ggtcaaagga aacatcatgt agcagcgtat
    10141 gcagattttg cgcttgctca ataccgcgcg cggtcagggg tgtgggcgca tgaccgctgt
    10201 acagaccatc gacattcgct tgcgtttcac catgacgaat taaccacagt cgcatcatgc
    10261 cctccgtaac gacaggtatc agcgatacaa gccttgttgg ttaatgtaag tcagtaccgg
    10321 ttccggtaat aaatcctcac acgattcacc attttgcaaa cgttcgcgga tgatggtcgc
    10381 cgagatgtta aaccacggcg tttcagccag ataaatttta ccggcaggct gaaggtgaag
    10441 atcttccggg ttatgtgtca aatgatcctc cagccattgc tgatattgtg gttgtgccat
    10501 ttcaagtggg taacctggac gccgacagac gatcaaatgt gcattgtcga gtatcgtttc
    10561 gtattcgtac caggtcggaa aggtcagcaa tgaatcctga ccaataataa acgccagcgg
    10621 cacgtccggt ccttgctcct gccgccactc tttcagtgtt tgcgcagtgt aagagggggc
    10681 attgcgcttt agctcgcgtt catcaagagt aaataatggc ttgtcggcaa tcgccagttc
    10741 aagcatgtgt ttacgctgca tgctgtttgc ttccggctgg ggacgatgcg gaggaacatt
    10801 attagggatg attgtgaccc gcgtcagacc aatcaaattc gccagcgttt ccacggggtt
    10861 aagatgacca tagtgcaccg gatcaaaggt gccgccaaac agagcctgta aagatttcat
    10921 atcaaccgtc gataaatacg tccgccaggg gtttatggca caacagaaga gataaccctt
    10981 caagctctgc ccacactgac tgaccgtaat cttgtttgag ggtgagttcc gttcgtgtca
    11041 ggagttgcac ggcctgacgt aactgcgact ggcttaagcg atttaacgcc tcgctcatca
    11101 tgccccggcg gttctgccat acccgatgct tatcaaacaa cgcacgcagt ggcgtatggg
    11161 cagactggcg tttcaggtta accagtaaca acagttcacg ttgtaatgtg cgcaacaaaa
    11221 taaccggttc gctgccttcc agacgcagtt gctgaagaat atgcaacgcg cgcttacttt
    11281 ttcccatcaa caaagcatca acccaatgaa aaggggtgaa atgcgcggca tcattcaccg
    11341 cctgttcaac gcgcggtaat gtcagtttgc cgtccggcca gagtagcgat aaacgctcca
    11401 gtgcctgggc cagcgccagc aggttacctt cataacagta gcagagcacc tgatttgcag
    11461 cgtcatccag ttctaagttg agctgttttg cgcgcgcagc aacccagcgg ggcagctgag
    11521 cctgctccgg tgtctgacag gtcacctgca cgctgcgatt cgcaagagca gtaaaccagg
    11581 cggcattttc ttgcgctttg cttaatttat taccgcggac gatcaacagt agatcgtcat
    11641 gcagaagtcc ggtgagtgtg agaagttgtt cattgatcgc cgcattcggt ccgttttcgg
    11701 gtaacaacag caatagcgtt tgtcgactgg caaacaggct catggcctgg cataacgaaa
    11761 aaatcgcatt ccagtcggtg ttgggatcaa tggaaaaagt gtggtgttct tcgaaccctt
    11821 gagccgcagc gacctgacga acagcgtcct ggctttcctg caataacaga ggatcgttac
    11881 caagtaaaag atacgccgcg cgcagccctt cattgagctg cgcgcggagt tgttccgggt
    11941 acaaccgaat catcagttac ccagcgtggt ggagacgcgt gcaggcgttg ccggagtatc
    12001 cgttgtggtc gacgtctgtt cttcgtcgga acgaatatcc gcagcacgga tgcttggcag
    12061 cttacgaatc agctgttcgg cagcacggtc gtacatctct tttacgatca tgtcttgttc
    12121 gttatctttc gctaacgcca tttgcgggtt atcgaagaac gaacggaaga ctttggcgct
    12181 aatcgggtag atgtcacggc cggggatcaa cacggtcgca ttcaccgtca tgatcatctg
    12241 atactctgct gtttgaccgt tacggaatac cgatgcggta tctttcgcga tgctcacttt
    12301 acccaaacgc aaggatggaa cgtccttacg cgtggtttct ttatcaagca actcgacacc
    12361 attcagacgt aactggttac gcaccgcacg gcttaatggc ccgttcggat cgcctgagtc
    12421 caggatcatg actttcatag tggaaggaac ctgcgtggtg tcacgcagat gccagccaca
    12481 cccggcggtg attaacaccg ccagagataa caacaatgtt gcgagagatc gcaagctgcc
    12541 tcctgcgcat agacaacgaa cagcatgatg agtcttcctt gtacgtcaa
//
LOCUS       DAWDRC010000035        12587 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2602_length_12587_cov_2.675790, whole genome shotgun sequence.
ACCESSION   DAWDRC010000035 DAWDRC010000000
VERSION     DAWDRC010000035.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12587)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12587)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_12587_cov_2.675790"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    10508..10517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    12359..12368
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2860 a         3185 c         3510 g         3012 t
ORIGIN      
        1 gttgaaggga tgcgcatgtc aaacctggtg accagcgtgg tcaaacatga cgaagaacgt
       61 cgtgttaacc cggacgacag caaggaggac taatgagtcg tcctcgtcgt cgcggtcgcg
      121 acattaacgg cgttttgttg ctggataaac cgcaggggat gtccagcaac gatgcgctgc
      181 aaaaagtgaa acggatttat aacgccaacc gtgccgggca taccggtgcg ctggacccgc
      241 tggcgaccgg catgttgcca atttgcctcg gggaagcgac gaagttttcc cagtatctgc
      301 tggactccga caaacgttat cgggtcattg cgcgtcttgg acagcgtacc gatacttctg
      361 atgccgacgg acaaatcgtt gaagaacgtc cggtaacctt tagtgcagag caactggcgg
      421 cggcgctgga taccttccgt ggcgatatcg aacagatccc ttcgatgtat tcagcactga
      481 aatatcaggg caaaaaactg tacgaatacg cgcgtcaggg cattgaagtt ccgcgtgaag
      541 cgcgcccgat taccgtttat gaattgctgt ttattcgcca tgaaggcaat gagctggagc
      601 tggaaattca ctgctcaaaa ggcacttata tccgcactat cattgacgat ctcggcgaaa
      661 aactcggttg tggcgcgcat gttatttact tgcgtcgtct ggcggtaagt aaatatccgg
      721 ttgaacggat ggtgaccctg gaacatctgc gtgaactggt tgagcaagcc gaacagcagg
      781 atattccggc tgcggagtta cttgatccat tactgatgcc aatggacagt ccagcttcgg
      841 actacccggt ggtgaatctt ccgttaacgt cttctgttta cttcaaaaat ggtaacccag
      901 ttcgtacatc cggtgcgccg ctggaaggac tggttcgcgt tacagagggt gagaacggca
      961 agtttatcgg tatgggcgaa attgacgatg aaggccgcgt tgcgcctcgt cgcctggtgg
     1021 ttgaataccc ggcgtaatgt taaccgtctt gcgataacag gtcgctacga gtagaatact
     1081 gccgcttaac gtcgcgtaaa ttgtttaaca ctttgcgtaa cgtacactgg gatcgctgaa
     1141 ttagagatcg gcgtcctttc attctatata ctttggagtt ttaaaatgtc tctaagtact
     1201 gaagcaacag ctaaaatcgt ttctgagttt ggtcgtgacg caaacgacac cggttctacc
     1261 gaagttcagg tagcactgct gactgcacag atcaaccacc tgcagggcca ctttgcagag
     1321 cacaaaaaag atcaccacag ccgtcgtggt ctgctgcgca tggtttctca gcgtcgtaaa
     1381 ctgctcgact acctgaaacg taaagacgta gcacgttaca cccagctcat cgggcgcctg
     1441 ggtctgcgtc gctaattctt gcgagtttca gaaaaggggg cctgagtggc cccttttttc
     1501 aagctgacgg cagcaattca ctggaaacta atgtattgtt gctatgaatg atcttccgtt
     1561 gcagaggttc gcgcggctaa tgagaggctt tacccacata gagctgggtt agggttgtca
     1621 ttagtcgcga ggatgcgcag aagatcgggt attaacacca gtaccgtaag gtactgtcta
     1681 agaaagagaa aggatattac attgcttaat ccgatcgttc gtaaattcca gtacggccaa
     1741 cacaccgtga ctctggaaac cggcatgatg gctcgtcagg ctactgccgc tgttatggtt
     1801 agcatggatg acaccgcggt atttgttacc gttgttggtc agaaaaaagc caaaccaggt
     1861 caggacttct tcccgctgac cgttaactat caggagcgta cctacgctgc tggtcgtatc
     1921 ccgggtagct tcttccgtcg tgaaggccgc ccaagcgaag gcgaaaccct gatcgcgcgt
     1981 ctgattgacc gcccgattcg cccgctgttc ccggaaggct tcgtcaacga agttcaggtt
     2041 atcgccaccg tggtttctgt taacccgcag gttaacccgg atatcgtcgc gatgattggt
     2101 gcttccgcag ccctgtctct gtctggtatt ccgttcaatg gcccgattgg tgctgcccgt
     2161 gtaggttaca tcaatgacca gtacgtactg aacccgactc aggacgaact gaaagagagc
     2221 aaactggacc tggttgttgc cggtacagaa gctgccgtgc tgatggttga atctgaagct
     2281 gaactgctga gcgaagacca aatgctgggc gcagtggtgt tcggtcatga acaacagcag
     2341 gttgttattc agaacatcaa tgaactggtg aaagaagccg gtaaaccgcg ttgggattgg
     2401 cagccggagc cggtaaacga agcgctgaac gcgcgcgttg ctgcgctggc tgaagctcgt
     2461 ctgagcgatg cttaccgcat caccgacaaa caagagcgtt atgcgcaggt tgatgtcatc
     2521 aaatctgaaa ccatcgcgac gctgcttgct gaagacgaaa ccctggacga aaacgaactg
     2581 ggtgaaattc tgcacgctat tgagaaaaac gttgttcgta gccgcgtact ggcaggcgaa
     2641 ccgcgtatcg acggtcgtga aaaagatatg atccgtggtc tggatgtgcg tactggcgtg
     2701 ctgccgcgta ctcacggttc tgcgctgttc acccgtggtg aaacgcaggc gctggttacc
     2761 gcaacgctgg gtaccgctcg tgacgcgcag gttcttgatg aactgatggg cgaacgtacc
     2821 gataccttcc tgttccacta caacttccct ccgtactccg taggcgaaac cggcatggtc
     2881 ggttctccga agcgtcgtga aattggtcac ggtcgtctgg cgaagcgcgg cgtgctggca
     2941 gtaatgccgg atatggacaa attcccgtac accgtacgtg ttgtgtctga aattaccgaa
     3001 tccaacggtt cctcttctat ggcttccgtg tgcggcgcgt ctctggcgct gatggacgca
     3061 ggtgtgccga tcaaagctgc cgttgcgggt atcgcaatgg gtctggtgaa agaaggcgac
     3121 aactacgttg tactgtctga cattttgggc gacgaagatc acctgggcga tatggacttc
     3181 aaagttgcgg gttcccgtga cggtatctct gcattgcaga tggatatcaa aattgaaggt
     3241 atcaccaaag agatcatgca ggttgcactg aaccaggcta aaggtgcgcg tctgcatatc
     3301 ctgggggtaa tggaacaggc gatcaacgcg ccgcgtggcg atatctctga gttcgcaccg
     3361 cgtatccata ccatcaagat caacccggac aagatcaaag atgttatcgg taaaggcggt
     3421 tctgtaatcc gtgctctgac cgaagaaacc ggcaccacca tcgaaattga agatgacggt
     3481 actgtgaaga ttgcagcgac cgacggcgag aaagcgaaac acgcgattcg tcgtatcgaa
     3541 gagatcactg cagaaatcga agtgggccgc gtctacaatg gtaaagtgac ccgtatcgtt
     3601 gactttggcg catttgttgc cattggtggc ggtaaagaag gtctggtcca catctctcaa
     3661 atcgctgata aacgcgttga gaaagtgacc gattacctgc agatgggtca ggaagtaccg
     3721 gtgaaagtgc tggaagttga tcgccagggc cgtatccgtc tgagcattaa agaagcgact
     3781 gagcagtctc aacctgctgc agcaccggaa gctccggctg ctgaacaggg cgagtaaggt
     3841 tgccatttgc cctccgctgc ggcggggggc ttttaaccgg gcaggacgcc ttgttagcaa
     3901 ccgggaacag gacgttcatt caaccgtggt cttcgggagt gggaaatgaa gccttttttg
     3961 cgctggtgtt tcgttgcgac agcacttacg cttgcaggat gcagtaatac ttcctggcgt
     4021 aaaagtgaag ttctcgcggt accattgcaa ccgactttac agcaggaagt gattctggca
     4081 cgtatggaac agatccttgc cagtcgggct ttaaccgatg acgaacgcgc acagctttta
     4141 tatgagcgcg gagtgttgta tgatagtctc ggtctgaggg cattagcgcg taacgatttt
     4201 tcgcaagcgc tggcaatccg accggatatg cctgaagtat tcaattactt aggcatatat
     4261 ttaacgcagg caggcaattt tgatgctgcc tatgaagcgt ttgattctgt acttgagctt
     4321 gatccaactt acaactacgc gcacttgaat cgcgggatcg cattatatta cggcggtcgt
     4381 gacaagttag cgcaagatga tctgctggcg ttttatcaag acgatcccaa tgatcctttc
     4441 cgtagtctgt ggctttatct cgccgagcag aagctcgatg agaagcaggc taaagaagtg
     4501 ttgaaacagc acttcgaaaa atcggataag gaacagtggg gatggaacat tgtcgagttc
     4561 tacctgggca acattagcga acaaacgtta atggaaaggc tcaaggcgga cgcaacggat
     4621 aacacctcgc tcgctgagca tctcagtgaa accaacttct atttaggtaa gtactaccta
     4681 agtctggggg atttggacag cgccacggca ctgttcaaac tggcggttgc caacaacgtt
     4741 cataactttg ttgagcaccg atacgcattg ttggaattat cgctcctggg ccaggaccaa
     4801 gatgacctgg cagaatcgga ccagcaatag ctgacgtaca catcagcccg taatcttttt
     4861 tgattgccat caccttaacg ggtgagggcg ttgttgttcg ttaatacacc tactttgagc
     4921 cggttcacac ttttcaatga aaattgctga tcaatttcat gatgagttat gtagactggc
     4981 cgccattaat tttgaggcac acgtactaca tggctgaatt cgaaaccact tttgcagatc
     5041 tgggcctgaa ggctcctatc cttgaagccc ttaacgatct gggttacgaa aaaccatctc
     5101 caattcaggc agagtgtatt ccacatctgc tgaatggccg cgacgtcctg ggtatggccc
     5161 agacggggag cggaaaaact gcagcattct ctttacctct gctgcagaat cttgatcctg
     5221 agctgaaagc accacagatt ctggtgctgg caccgacccg cgaactggcg gtacaggttg
     5281 ctgaagcaat gacggatttc tctaaacaca tgcgcggcgt aaacgtggtt gccctgtacg
     5341 gcggccaacg ttatgacgtg caattacgcg ccctgcgtca ggggccgcag atcgttgtcg
     5401 gtactccggg ccgtctgctg gaccacctga aacgtggcac tctggacctc tctaaactga
     5461 gcggtctggt tctggatgaa gctgacgaaa tgctgcgtat gggcttcatc gaagacgttg
     5521 aaaccattat ggcgcagatc ccggaaggtc atcagaccgc tctgttctct gcaaccatgc
     5581 cggaagcgat tcgtcgcatt acccgccgct ttatgaaaga gccgcaggaa gtgcgcattc
     5641 agtccagcgt gactacccgt cctgacatca gccagagcta ctggactgtc tggggtatgc
     5701 gtaaaaacga agcactggta cgtttcctgg aagcggaaga ttttgatgcg gcgattatct
     5761 tcgttcgtac caaaaacgcg actctggaag tggcagaagc ccttgagcgt aacggctaca
     5821 acagcgccgc gctgaacggt gacatgaacc aggcgctgcg tgagcagaca ctggaacgac
     5881 tgaaagatgg tcgtctggac atcctgattg cgaccgacgt tgcggcccgt ggcctggacg
     5941 ttgagcgtat cagcctggtg gttaactacg atatcccgat ggattctgag tcttacgttc
     6001 accgtatcgg tcgtaccggt cgtgcgggcc gtgctggccg tgcgctgctg ttcgttgaga
     6061 accgtgagcg tcgtctgctg cgtaacattg aacgcaccat gaagctgacc attccggaag
     6121 tagaactgcc gaacgcagaa ctgctgggca aacgtcgtct ggaaaaattt gccgctaaag
     6181 tacagcagca gctgtaaagc agcgatctgg atcaataccg tgcactgctg agcaaaattc
     6241 agccgactgc cgaaggtgaa gagctggatc tcgaaactct ggcagccgca ctgctgaaaa
     6301 tggcacaggg tgaacgtact ctgattgttc cgccagatgc gccgatgcgt ccgaaacgtg
     6361 aattccgtga ccgtgatgac cgtggtccgc gcgatcgtaa cgaccgtggc ccgcgtggcg
     6421 accgtgaaga tcgtccgcgt cgcgaacgtc gtgatgttgg cgatatgcag ttgtaccgta
     6481 ttgaagtggg ccgcgatgat ggtgttgaag ttcgtcatat cgttggtgcg attgctaacg
     6541 aaggcgacat cagcagccgt tacatcggta acatcaagct gtttgcttct cactccacca
     6601 tcgaactgcc gaaaggtatg ccgggtgaag tgctgcaaca ctttacgcgt actcgcattc
     6661 tcaacaagcc gatgaacatg cagttactgg gcgatgcaca gccgcatact ggcggtgagc
     6721 gtcgtggcgg tggtcgtggt ttcggtggcg aacgtcgtga aggcggtcgt aacttcagcg
     6781 gtgaacgccg tgaagggggt cgtggtgatg ggcgtcgttt tagcggcgaa cgtcgtgaag
     6841 gccgcgcacc gcgtcgtgat gactctaccg gtcgtcgtcg tttcggtggt gatgcgtaat
     6901 catcgctgaa cagcgaacac aatctgtaaa ataatatata cagccccgat tttaaccatc
     6961 ggggcttttt ttctgttttt tgtactcatg tactggtaca gtgcaatgca taacaacaca
     7021 gtcgcactat ttttcactgg agagaagccc tcatggcaac actaaccacc acccaaacgt
     7081 caccgtcgct gcttggcggc gtggtgatta tcggcggcac cattattggc gcagggatgt
     7141 tttctctgcc tgtggtcatg tccggggcgt ggttcttctg gtcaatggcg gcgctgatct
     7201 ttacctggtt ctgtatgctg cattccggct tgatgattct ggaagctaac ctgaattatc
     7261 gaatcggttc tagctttgac accatcacca aagatttgct gggcaaaggc tggaacgtag
     7321 tcaacggcat ttccattgcc tttgtgctct atatcctgac ttacgcctat atttctgcca
     7381 gcggttcgat tctgcatcac accttcgcgg agatgtcgct gaacgtccct gcacgggcag
     7441 ctggatttgg ttttgcactg ctggtagcgt tcgtggtgtg gttgagtacg aaagctgtca
     7501 gccggatgac agcaattgtg ctgggggcga aagtcattac tttcttcctc accttcggca
     7561 gcctgctggg gcatgtgcag cctgcgacat tgttcaacgt cgccgaaagc aatgcgtctt
     7621 atgcaccgta tctgttgatg acgctgccgt tctgtctggc atcgtttggt tatcacggta
     7681 acgtaccgag cctgatgaag tattacggca aagatccgaa aaccatcgtg aaatgcctgg
     7741 tgtacggtac gctgatggcg ctggcgctgt ataccatctg gttgctggcg acgatgggta
     7801 acatcccgcg tccggagttt atcggtatcg ccgagaaggg cggtaatatt gatgtgctgg
     7861 tacaggcgtt aagcggcgta ctgaacagcc gtagtctgga tcttctactg gtcgtgttct
     7921 caaactttgc ggtagcgagt tcgttccttg gcgtaacgct gggtttgttt gactatctgg
     7981 cagatctgtt tggttttgac gactcggcta tgggccgttt gaaaacagcg ttgctgacct
     8041 ttgccccgcc tgttgtgggt ggcctgctgt tcccgaacgg attcctgtac gccattggtt
     8101 atgctggctt agcggctacc atctgggcgg caattgttcc ggcgctgttg gcccgcgcat
     8161 cgcgtaaacg ctttggtagc ccgaaattcc gtgtatgggg cggcaagccg atgattatgc
     8221 tgattctggt atttggcgtt ggcaacgcac tggtccatat cttatcgagc tttaatgtgc
     8281 tgccggtgta tcagtaatca gcggtgcctt atccgacatt tctgctgcct acacaatgcc
     8341 tgatgcgctt cgcttatcag gcctacgtag gacagcgttg ccatctcgga taaggcttcc
     8401 tacgttaaaa cacactaccc caacaactct tccttcacat ccatcgccag ctcaaacgaa
     8461 tgcaggcgtg cctgatggtc gaaaatctgc ccgttaacca taatttcatc ggcgtcggtt
     8521 tcgcgcagga tcgactgcaa gccgtgacgc actttcgctt tatcgcccac cagcgacata
     8581 ctcagcgcct gctgcacacc atactgttcg gacggcgacc agaactgatc catattttga
     8641 atcggtggcg gcagttgccc ggtttcgccg cggcgcagct tcacaaaggc ttgctgcatt
     8701 gaggtaaaca ggaattcggc atcgcggttg ctgtcggcgg cgataatatt gatgcacacc
     8761 atcgcatacg gtttctccaa ccgtgccgac ggtttgaagt tgctgcgata aagatgcagc
     8821 gcctggaaca gcatatccgg cgcgaagtgt gaggcaaacg caaacggcag accaagctgc
     8881 gccgccagtt gcgcgctgta aaggctggat cctaacaacc acacggggat tttctcgcca
     8941 tagcctggta ctgggcgcac atgcggattg ggatcgcggg cgtcaaacca gtccaccagt
     9001 tccgcgacat cgcgggggaa attatcaata tcgccgctca tatgacgacg cagcgccatc
     9061 atggttcgct gatcgctacc cggcgcgcgg cccagcccca aatcgattcg ccccggatag
     9121 agcgtattga gcgtgccgaa ttgttcggcg atcaccaacg gtgagtggtt aggcaacatc
     9181 acgccgccag accccagatg cagcgtggtg gtattcgccg ccagatagcc aattaacacc
     9241 gacgttgcag cactggcaat gccggtcata ttgtggtgtt ccgccagcca gtagcgatga
     9301 tagccgcgct tttcagccag acgggcgagg tcgagagagt gggagaacgc ttcccgcgct
     9361 gaagaacctt cggggatggg ggccagatcg agtagcgaaa acgcaatggt tttatcagtc
     9421 ataacagctc actttgttac cgattaatct ttaatactgc attaaagagt agttaaagtt
     9481 gttaacaaag tgagctatat acttacgctt gcagctccag cccggccagc cgccgccagt
     9541 agccgttaca gtcgctgttt gccgtcaacg gtagtggcgc agcgccattt tcattagcgc
     9601 ggaaggcatc gagcattgcg aaagtgtcag taccctgcgg tgacaggcgg accacatcga
     9661 ccagcccctg catggatgcc agctcgttac cgaggttgta cacgtagccg ctcatggtct
     9721 gaatgccatt gagcacaaac acttgttggt tttcctgcga cagcacgttg cgcccgttcg
     9781 gatacttaat gcagcaggtt tcacactcat ctttcgggcg gtcttccgaa cgcgcggtaa
     9841 agcagcgggc ggagtaggcc agcggtagat gaccgtagct caggacttcc acttcaaact
     9901 ggttgcgaat gcccaactca tcgcactgat taagcagatt caccagccag tcgcgggaaa
     9961 gctccaccgg catacaccag cgcatcatgc cctgtttgag caatattttc agtgtcaccg
    10021 cgttgtagca gttcagcgcg tgcccggcga cgaacggcag tttgcgttca gcgcacatat
    10081 tcaccacgcc gagatcgctg gcttcaatca gaaactcacc gttctcaaca tagcgtttca
    10141 gttcgcccag ttcggaggat gcctgcacca gcgccagcgt ggagagcaca atctgcttgc
    10201 cacttccggc gagcgatttc gccatctcca gccagtcgcc aactttggtt gcccggcgtt
    10261 tgctacagac cgcttcaccg agataaatca cgtctgcgct gctggcggcg gcctgctgat
    10321 aaaaatcttc cagcgtctct tttggccagt accacagcac tggccctaag gaatatttca
    10381 ttgcttttct cactgccatt tacggtgata cgcgccaagg gtggtctgcg tgccttcgga
    10441 catcgacccg agcgtctcca tccacgcgct ttgcggtacg aagttttgcg gatcggcctt
    10501 acaacggnnn nnnnnnngat agcctgacgc cagacttttg ccacctggct gacatacgcc
    10561 gggctacgct ggcggccttc aattttcacc gaagcgatat tcgccgccat taactccggc
    10621 agcagttcca gggtattgag actggttggt tcttccagcg cgtgatagcg ctcgccgtcc
    10681 accagataac gccctttaca cagcgtcgga taacctgcgt tttcgccgtc ctgataacgg
    10741 tcgatcagca cttcgttcag gcgggattcc agcccctgcg gcgtttgctg ccagcgcacg
    10801 aagcgagccg gagaacatgc accgacggtg ttgggcgatt cacccgtcag atatgacgac
    10861 agatagcaac ggccttccga cataatgcac aggctgccga aagcaaagac ttccagtggt
    10921 acaggtgtga ctcgtgccag ttgtttcacc tgatgaatcg acaacacgcg cggcagcacc
    10981 acgcgggcaa cgtcaaaatg gcgatgataa aagtgaatcg cctcttcatt ggtcgccgaa
    11041 gcctgcactg acacatgacg ctcaatatgc gggtatcgct cggcggcgta ctccagcatg
    11101 gcgagatcgg cgaggatcag cgcgtcggca cccagttgcg ccgccatatc cacggcacgc
    11161 tgccaacggg cgtaaccgtc cggatgcgca aaagtgttaa tcgcgatgtg aagtttgcgg
    11221 cgatgttgat ggacaaaact caccgcttcc tgcaattttt tctcggtaaa gttaaggccg
    11281 gcgaagtgac gggcattggt atcatctttt agcccgatat aaacagcatc tgcgccgttt
    11341 tcgatggccg ccttaagcgc cgggagattt ccggcagggc agagcagctc cataattttt
    11401 cctgaaggtt gcgctatcaa aacgcaaaaa tgttaacgaa ctgggatttt agttaacctt
    11461 gctgcgacaa tttttgattt aaggcagtta aagttgtatt ggtggtagca gcaatttcat
    11521 atggaattgt tgatttatac cgctatgtta tttttctgat atggcaaaat aggatgattg
    11581 ttgaaacagg gagtaaaact cgtgttggat aaactgcgtt cccgtattgt gcatttgggg
    11641 ccatctctgt tgagtgtacc ggtaaaactg acgccatttg cgctaaaacg ccaggttctt
    11701 gagcaggtct taagctggca attccgccag gcgctggatg atggcgagct ggagtttctt
    11761 gaaggccgct ggttaagtat tcatgtgcgt gatattgacc tgcaatggtt tacctcggtg
    11821 gtgaatggca aactggtggt cagccagaac gcgcaagctg atgtgagttt tagtgccgac
    11881 gccagcgatc tgctgatgat tgcggcgcgt aaacaagatc cggatacgct cttcttccag
    11941 cgtcggctgg tgattgaagg cgatacggag ctggggctgt atgtgaaaaa cctgatggac
    12001 gccattgaac tggagcaaat gccgaaagcc ttacgcatga tgctgctgca actggcggat
    12061 tttgttgagg cgggaatgaa aaccgcgcct gaaaccaaac agacatcggt aggtgaacca
    12121 tgctaattcg agtagaaatt cccattgatg cgccgggtat tgatgccctg ctgcgtcgtt
    12181 cattcgaaag tgatgcggaa gcgaagctgg ttcacgatct gcgtgaagat ggctttctga
    12241 cgctggggct ggtggcgaca gatgacgaag gtcaggtcat tggctatgtg gcttttagtc
    12301 cggttgatgt gcagggcgaa gacctgcaat gggtcggcat ggcaccgctg gcggtagann
    12361 nnnnnnnnta ccgtggacag gggctggcac gccaactggt ctatgaagga ctcgattcgc
    12421 ttaatgagtt cggctatgcc gcagtggtga cgctgggcga tccggcgctg tacagccgtt
    12481 tcggctttga actggcggcg catcacgatc tgcgttgccg ctggccgggc actgaaagcg
    12541 ccttccaggt acatcgttta gcggatgacg cgctgaacgg cgtaacc
//
LOCUS       DAWDRC010000036        12337 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2664_length_12337_cov_2.724801, whole genome shotgun sequence.
ACCESSION   DAWDRC010000036 DAWDRC010000000
VERSION     DAWDRC010000036.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12337)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12337)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2664_length_12337_cov_2.724801"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    12031..12040
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3029 a         3394 c         3154 g         2750 t
ORIGIN      
        1 tgatagagat caccttgcag ttgtaatcac gctcaacttc ctgaatcgcc gaaatttcgc
       61 cgcgcccgcg ttcctgacga tcaagcgaaa tcaacacgcc tgcaagcgtc gcgccattgg
      121 cctgaataat ttccatcgac tcgcgaatcg ccgttccggc ggtgatcaca tcatctacca
      181 gcattacgcg tccttgtaac gcgctaccaa ccagattacc gccttcaccg tggtcttttg
      241 cctctttgcg gttaaagcag tacggcaggt caaggtcatg atgctccgcc agcgccacgg
      301 cggtagtggt ggcaatcggg atccctttat aagcagggcc aaacagcaga tcgaactcaa
      361 tgccggaatc caccaatgct tcagcgtaaa aacggcctaa cagtgccaga tcgcgcccgg
      421 tattaaacag cccggcgttg aagaaatagg ggcttttgcg cccggatttc agcgtgaact
      481 cgccaaactt taacacctgc ttgctaagcg caaattcaat aaactggcgc tgatatggtt
      541 tcatgccttc gctcctcatc ttacttttct acagacaaaa aaaaggcgac tcatcagtcg
      601 ccttaaaaat cagtttgcca gcgccgcctt ctgcgtcgct acaatggatt cgattccccc
      661 tcgggccagc gccaacaagg tgagtaactc ttcgtgggtg aacggttcgc cttctgccgt
      721 cccctgcact tcaatgatac gcccgtcttc ggtcatcact acgttcatgt cggtctctgc
      781 ggcagagtct tcaacatatt ccagatcgca aaccgcttca ccgttcacaa tcccgacaga
      841 gaccgcggct accatccctt tcattggatt agttttcagc ttgccgtttt ccaccagctt
      901 ctgtagcgca tctgccagcg ccacgcaggc acccgtaatc gaagcggtac gcgtgccacc
      961 atcagcctga agcacgtcgc agtccagcgt aatggtgaac tcacccagtg ctttcaaatc
     1021 tactgccgcg cgaagagcac gggcgatcag acgctggatt tccattgtgc gtccaccctg
     1081 cttacctttc gccgcttcac gcgcgttacg ggtatgggta gaacgtggca gcataccgta
     1141 ctctgcggtg atccagccct ggccctgacc tttcaggaag cgcggcacgc cttcttcaat
     1201 agaggcggta cacaatactt tggtatcgcc aaattcgacc agcaccgagc cttctgcatg
     1261 ttttgtatag ttacgagtca gggtaacggg acgcacctga ttattgctac ggcctgctgg
     1321 acgcatattg aaatctccgg cttgaaacaa atgtggctgc gcattatacg gacttccggc
     1381 ggttattcct atcctgacaa ggcatcgatg gctataatcc ttccacctct ccttttataa
     1441 acaggaacgt ctatgatccg cagtatgacc gcctacgccc ggcgtgaaat caagggtgaa
     1501 tgggggagcg caacctggga aatgcgctcg gtaaaccagc gttatctgga aacttacttt
     1561 cgtctgccgg agcagttccg tagccttgaa cctgtcgttc gcgagcgtat tcgttctcgc
     1621 ctgacgcgcg gtaaagtgga atgtactctg cgctatgagc cagatgttag cgcgcaaggt
     1681 gagctgatcc tcaacgaaaa actggctaaa cagctggtaa ctgccgcgaa ctgggtaaaa
     1741 atgcagagcg acgaaggaga aatcaacccg gttgatattc tacgctggcc gggcgtgatg
     1801 gcagcccagg agcaagatct cgacgcgatt gcagctgaaa ttctcgcggc gttggatggc
     1861 accctggatg actttatcat cgcgcgcgaa accgaaggtc aggcactgaa agtgttgatt
     1921 gagcagcgtc tggaaggcgt caccgccgaa gtggccaaag tccgcgccca tatgccggaa
     1981 atcctgcaat ggcagcgtga gcgtctgata gcgaagctgg aagatgctca ggtgcaactg
     2041 gaaaacaacc gtctggagca ggagttggtt ctgctggcac aacgaattga cgttgccgaa
     2101 gaactggacc gcctcgaagc gcatgtcaaa gagacctaca acattctgaa gaaaaaagaa
     2161 gccgttggtc gtcgtctgga ctttatgatg caggagttca accgcgagtc gaacactctt
     2221 gcgtcgaaat ctatcaacgc cgaagtgaca aactccgcca tcgagctgaa agtgttgatt
     2281 gagcagatgc gcgagcagat tcagaatatt gagtaagcga catatctccg taggccgttg
     2341 ttaattacag cggcgctacg catatagttt cgtcctcata tttatttact ccatactttt
     2401 tctaatgcat tttacttatc acaaaatcag aaaaatgcat tcttactaca gaaaatagca
     2461 atttcacata actacaaatt atcaatctat tctctcctta aaaaatctca atcgtgacaa
     2521 tgcgcacaaa tcgctaccct tccagacaga tttttaggga gagaaccatg ctgttacaca
     2581 ttttgtattt ggttggcatt actgccgaag ccatgacggg ggcgctggcg gccggacgac
     2641 ggcgcatgga tacatttggc gtaattatta ttgcgaccgc cacggcaatt ggcggagggt
     2701 cagtgcgcga tattctgctg ggccactatc cgctcggctg ggtcaaacac ccggaatatg
     2761 tgattatcgt cgctaccgct gcagtactca ccactatagt tgctcctgta atgccttatc
     2821 tgcgcaaagt gtttctggtg ctcgatgcgc tcggactggt ggtcttttct atcatcggcg
     2881 cacaggttgc actggatatg gggcacggcc cgattattgc cgttgtagcg gcggtaacca
     2941 ccggcgtgtt tggcggcgtt ttgcgcgata tgttctgtaa acgtattcca ctggtattcc
     3001 agaaagagtt gtatgccggt gtctcatttg cctccgccgt gctgtacatc gcgctgcaac
     3061 actatgtttc taaccatgat gtggtgatta tctccaccct ggtattcggc ttttttgccc
     3121 gtttactggc gctacgtctt aagctgggat taccggtctt ttactacagc cacgaaggtc
     3181 actaaggttc aaaacctgtg atctgctggg cagacagcca actgcccagc ttcttgattt
     3241 gcgcattttc cttccattca ataacctgtc tggcgcgccc cgatccagtc cccggcagct
     3301 gctgccagaa ctgttccgtg ctaaataaaa gttgcgacca ggaccgttca tcactggcat
     3361 taagcgccgc ccgggttagc ggtattccca ttgccatcac ccagcgagta aaaggctgct
     3421 gacgagccag attaaactga tgccataact gcgcactttt acttttcgcg atccccggcg
     3481 tgttctgtaa ttgctctggc gttaataaaa gccaggaaaa gatatgctca aagcgatgag
     3541 tctgatgcag ggcgcgccag ccggcctcac caatgccatc aagccccaga acctgttttg
     3601 accccagcca gactaagcgt gaaatgaact gttcctgaca aacatcagaa gcaaagtagc
     3661 aggtcaacga gttaaagcgg ttttctggtg gtgtcggttt tgtacgttct gctccgcgcc
     3721 agaccacatc atcaatgcga ggaatcccct gaccggcaag gctgacgagg atctgatcac
     3781 caggcgcaat atcccactcc tgccagcgcc tgacggaacc aatattcacc cgctggactt
     3841 ttttatcatc cagcatgaca ggtacaagtg acgcaaccac cgatatttta ccgctcttac
     3901 ccaccgcaaa ctgaatcgcc ttcacttcgg caacctgagc tacaggttga tatttccagg
     3961 ccaccagcca ttctgcctgg cttggtagcc aatggcggga ttcgggttct ttcgccgctc
     4021 gcacaaccac gccatcggtg acgaagggta atttcgtttt ccaccacgca ttgcgtacgc
     4081 gcgcaacttc atcagcattt ttcaccgcac gggtatacgt ctgcgttaga gtaaaaccag
     4141 cggtagccag ctcttttaaa cgatcggtca ttaactgcgg tccatccggc catgcccaga
     4201 caaaaacgcc cagagaattc agcgtgtcgc tatcgtcctg gcgcatcatc aggccagcaa
     4261 cttttgcgcg ggcatttatt ccccccattt gttgttggat atgcccctcg cgtttgagaa
     4321 atatttcccc ctgaagcgta ctgttggcta aaggcccgct aacggtttgc ggcacagcgg
     4381 aaattaagcg aactttctgc gtccagtcct cgcctttcag gccattgccg cgactgattg
     4441 ctttgttcag tttcccgtcc cgataaacca gggttaccgc aacgccatca acttttggct
     4501 gcacccaaag atcgctacgt tcgcgcatcc acagacttaa tgcgatttta tccgccattt
     4561 tgcgtacacc cgtatgtgca accggatgta taaccgcacc gtttaaaggc ggcatcatag
     4621 cgtcgcgatg cgtctcttca ccaaaacagc gttgccactg cgtgagacgg gcgcttaact
     4681 gatcgtaaac cccgtcttcc acctcacttt tcccttcttt ccagtagtca tcgtcccact
     4741 gttttatttg ctgttgcagg cgggaaattt cttcctgtgc tcgggctggc gaccaggccg
     4801 gacagaccgc ccacgccgat gattgccagc acaagatact tattaatatc gccatccata
     4861 ctttcatcat cacctccgct gtagatagtc aggcagatat acaacgtgat gaaagcaaag
     4921 ccgagtggca aaaacggagt ctgcgaggac gcttcctgag aatcgtcttt attgcagtga
     4981 atcacaggca aatgcggaag cagctacgca aaatgcaaca actttgcgca aaaagtgtga
     5041 gcaagggcta cgtcacatgg ccgcgccgtg tataataagc tcgtatgtag gctttatttc
     5101 gctaatcaca tacgaaagat actcatggct caaggcacgc tttatattgt ttctgccccc
     5161 agtggcgcgg gtaaatccag cctgattcag gctttattaa aaacccaacc gttgtatgac
     5221 acccaggttt ctgtttcaca caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa
     5281 cattatttct ttgttaatca tgatgaattt aaagaaatga ttagtagaga tgcgttcctc
     5341 gaacacgcag aagtttttgg taattactat ggcacttcgc gtgaggccat tgagcaagta
     5401 ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc agggtgcgca gcaaattcgc
     5461 cagaagatgc cgcacgcgcg gagtatcttt attttaccgc cgtctaaaat cgaactggac
     5521 cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa
     5581 gctgttgcag aaatgagcca ttacgccgaa tatgattacc tgattgtgaa tgatgacttc
     5641 gataccgcgt tgaccgattt gaagaccatt attcgcgccg aacgtctgcg catgagccgc
     5701 caaaagcagc gtcatgacgc tttaatcagc aaattgttgg cagactgaac ctgatttcag
     5761 tatcatgccc agtcatttct tcacctgtgg agctttttaa gtatggcacg cgtaactgtt
     5821 caggacgctg tagagaaaat tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc
     5881 gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac cggaagaaaa cgataaaacc
     5941 actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca acaaccagat cctcgacgtt
     6001 cgcgaacgcc aggaacagca agagcaggaa gccgctgaat tacaagccgt taccgctatt
     6061 gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt atctgtttga aagcctgaat
     6121 cagctgattc aaaactacct gccggaagac caaatcaagc gtctgcggca ggcgtatctc
     6181 gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac
     6241 ccggtggcgg ttgcctgcat tctggccgag atgaaactcg actatgaaac gcttatggcg
     6301 gcgctgctgc atgacgtgat tgaagatact cccgccacct accaggacat ggaacagctt
     6361 tttggtaaaa gcgtcgccga gctggtagag ggggtgtcga aacttgataa gctcaagttc
     6421 cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga tgattatggc gatggtgcag
     6481 gatatccgcg tcattctcat caaacttgcc gaccgtaccc acaacatgcg cacgctgggc
     6541 tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa cactcgaaat ttacagcccg
     6601 ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac tcgaagagct gggttttgag
     6661 gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac
     6721 cgtaaagaga tgatccagaa gattctttct gaaatcgaag ggcgtttgca ggaagcggga
     6781 ataccgtgcc gcgtcagtgg tcgcgaaaag catctttatt cgatttactg caaaatggtg
     6841 ctcaaagagc agcgttttca ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat
     6901 gattccgaca cctgttatcg cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg
     6961 ggtcgcgtga aagactacat cgccattcca aaagcgaacg gctatcagtc cttgcacacc
     7021 tcgatgattg gcccgcacgg cgtgccggtt gaggtccaga tccgtaccga agatatggat
     7081 cagatggcag agatgggtgt tgccgcgcac tgggcttata aagagcacgg cgaaaccagc
     7141 accaccgcgc aaatccgcgc ccagcgctgg atgcaaagcc tgctggagct gcaacagagc
     7201 gccggtagtt cgtttgaatt tatcgagagc gttaaatccg atctcttccc ggatgagatt
     7261 tacgttttca caccggaagg gcgcattgta gagctgcctg ccggtgcaac gcccgtcgac
     7321 ttcgcttatg cggtgcatac cgatatcggt catgcctgcg tgggcgcacg cgtcgaccgc
     7381 cagccttacc cgctgtcgca gccgcttacc agcggtcaaa ccgttgaaat cattaccgca
     7441 ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg tcagctcgaa agcgcgcgcc
     7501 aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt
     7561 ctgctcaacc atgcgttggg tggtagccgt aagctcaatg aaatcccgca ggaaaatatt
     7621 cagcgcgagc tggaccgcat gaagctggca acgcttgacg atctgctggc agaaatcggc
     7681 cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt
     7741 ccaccggcaa cccaaagtca cggacatctg cccattaaag gtgccgatgg cgtgctgatc
     7801 acctttgcga aatgctgccg cccaattcct ggcgacccga ttatcgccca cgtcagcccc
     7861 ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaaca tccgtggcta ccagaaagag
     7921 ccagagaagt ttatggctgt tgaatgggat aaagagacgg cgcaggaatt catcaccgaa
     7981 atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa acctgacggc ggcaattaac
     8041 accacgacct cgaatattca aagtttgaat acggaagaga aagatggtcg cgtctatagc
     8101 gcctttattc gtctgaccgc ccgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc
     8161 cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa attaatgttt tatgaaccca
     8221 acacgttatg cacgcatctg cgaaatgctc gccaggcggc agcctgatct gaccgtctgc
     8281 atggagcagg tccacaaacc tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt
     8341 ggcgtacatg aagttcatgc tgtctggcct ggcagccgga tgcgcaccat ggcttcggca
     8401 gctgcgggta gtaacagctg ggtacaggtg aaaacgcacc gcaccattgg cgatgccgtc
     8461 gctcatctga aaggccaggg catgcagatt ctggcaaccc atctttctga taacgctgtt
     8521 gatttccgcg aaattgatta cactcgcccg acctgtattt tgatgggaca ggagaaaacg
     8581 ggcatcacgc aggaagcgtt ggccctggcg gatcaggata tcatcattcc gatgatcggc
     8641 atggtgcagt cgctgaatgt ttccgttgcc tcagccctca ttctttacga agcccagcgc
     8701 cagcggcaaa atgcgggcat gtacctgcgt gaaaacagca tgttgccgga agccgagcaa
     8761 caacgcctgc tgtttgaagg cggctatccg gtgctggcga aagtcgcaaa acgcaaaggt
     8821 ctgccctacc cccacgttaa tcagcaaggc gagattgaag ccgatgctga ctggtggtcc
     8881 accatgcagg ccgcagggta agcgcgatga aaggtcgcct gttagatgct gtcccgctca
     8941 gttccctaac gggcgttggc gcagcgctta gtaacaagct ggcgaaaatc aacctgcata
     9001 ccgtacagga tttactctta caccttcctc tgcgctacga agatcgcaca catctctacc
     9061 ccatcggtga actactgccg ggtgtttatg ccacggtgga aggcgaagtg ctgaactgca
     9121 atatctcctt cggcggtcgg cggatgatga cctgccagat cagcgacggt tccggcattc
     9181 tcactatgcg ctttttcaac ttcaatgcgg caatgaaaaa tagcctggcg acaggccgcc
     9241 gcgtgctggc ctatggcgaa gcaaagcgcg gcaaatatgg tgcggagatg atccacccgg
     9301 aataccgtgt gcagggcgat ctcagcacgc cagagttaca ggaaacgctc acaccggttt
     9361 atccaactac ggaaggcgta aagcaggcta cgctgcgtaa attaaccgac caggcgctgg
     9421 atctgctcga cacatgcgcc attgaagaac tcctgccgcc ggaactgtca caaggaatga
     9481 tgacgctacc ggaagcgttg cgcactttgc atcgcccacc gccgacgctg cagcttagcg
     9541 atctcgaaac cgggcagcat ccggcacaac gccgtctgat tctggaagag ttgctggcgc
     9601 acaacctcag catgttggcg ttacgtgccg gtgcacaacg ttttcatgct cagccgctga
     9661 gcaccaatga cgcgctgaaa aataaactcc tcgccgcctt accgttcaag ccaacgggcg
     9721 cacaggcacg cgtagtggcg gagatcgagc gcgatatggc gctggatgtg ccaatgatgc
     9781 gcctggtgca gggcgatgta ggttccggta aaacgctggt cgctgccctc gccgcgttgc
     9841 gtgcggttgc ccacggcaaa caggtggccc tgatggcacc aaccgaatta ctcgccgagc
     9901 agcacgccaa taactttcgt aactggtttg caccgctcgg tatcgaagta ggctggctcg
     9961 ccggaaaaca gaaaggtaaa gcacgactgg ctcagcaaga agccatcgcc agcggtcagg
    10021 tgcagatgat tgttggtacg cacgccattt tccaggaaca ggtgcagttt aacggcctgg
    10081 cgctggtgat tatcgacgaa cagcatcgtt ttggcgtaca tcagcgtctg gcgttatggg
    10141 agaaaggcca gcagcagggc ttccatccgc atcagttgat catgaccgcc acgcccatcc
    10201 cccgcacgct ggcgatgact gcgtatgccg atctcgatac ctcggtgata gatgagctgc
    10261 caccaggccg cacgccagtg actacggtcg ctattcctga tacccgccgt accgatatca
    10321 ttgaccgcgt gcgccacgcc tgtatgactg aagggcgtca ggcgtactgg gtttgtacgt
    10381 tgattgaaga gtcggaatta ctggaagcgc aggcggcgga agcgacctgg gaagagttga
    10441 aactggcgct accagagttg aatgttggcc tggtacacgg acggatgaaa cccgccgaga
    10501 aacaggcggt gatggcgtcg tttaaacaag gtgaactaca cctgctggtt gccactactg
    10561 tcattgaagt cggtgttgat gtacccaatg ccagtctgat gattatcgaa aacccggagc
    10621 gtctgggtct ggcgcagtta caccagttac gtggccgcgt gggtcgtggc gcggtggcct
    10681 ctcactgcgt gctgctctac aaaacgccgc tctccaaaac cgcgcaaatc cgcctgcagg
    10741 tactacgcga cagcaacgat ggttttgtga ttgcacagaa agatctggag attcgcggcc
    10801 ctggcgaatt gttaggtaca cgtcagacgg gtaatgctga atttaaagtg gcggatttac
    10861 tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc gcgacatatt cacgaacgtt
    10921 acccacaaca ggcaaaagcc ctgatagaac gctggatgcc agaaacagaa cgttactcga
    10981 atgcgtaaaa agcggcggtg agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc
    11041 aacatcaaat acagcttaat caccagcgca ttgacgatat cgataaagaa cgcaccgacc
    11101 atcggcacga ccaggaacgc catgtgcgaa gggccaaagc gttcagtgat cgcctgcatg
    11161 ttagcgattg ccgttggtgt cgcaccaagg ccaaagccac agtgacccgc agccagcact
    11221 gccgcatcgt agtttttgcc catcatgcgc caggtaacga agatggcata caacgccatg
    11281 aagatggtct gtaccaccag aatcgccagc atcggcaacg ccagcgaagc cagctcccac
    11341 agtttcagcc ccatcagcgc catcgccagg aacaacgaca ggcttacgtt acccagcacg
    11401 gataccgctc gctcaaagac gcggtaaaag cccatcattg acagaccgtt gctcagaatc
    11461 acgccaacaa atagtacaca gacgaaggtc ggtagttcaa aagcagtacc agccaaaagt
    11521 tgcgcaacaa ttttccccac tgtcaggcag atagcaatca gcgcgatagt ttcaatcagc
    11581 accagcgagg tgatcatgcg gcccacatcc ggcttttcga acgcagtcgg aacttcctgg
    11641 tcatccggaa taccgttcgg tgtggtggag tgtttcacca gatagcgcgc caccggaccg
    11701 ccaatcaagc cgcccagcac caaaccgaac gttgcacagg ccatcgcccc ttctgtcgcg
    11761 ttggtgaagc cataacgttc aatgaacaat ttactccatg cagcacccgt accgtgaccg
    11821 ccggaaagcg taatagaacc ggccagcagc cccatcagcg gatcaagccc taacaggcta
    11881 gccataccaa tgccaatggc attttgcatc accaacaggc caacaaccac aatcaggaaa
    11941 acccccacca cacgcccacc agctcgcaga ctggcaaggt tggcgttcag gccaatggtg
    12001 gcgaagaaag ccagcatcaa cggatcgcgc nnnnnnnnnn gcaacaggtt ccggtatggt
    12061 gtatttcttc aaaaaggaga cggaatggac caacttgcgc ccgagcagca acgtcagcgt
    12121 tgcggcaaca agcgttgcta aagtatcgag atgaaacata gttactcctt tgtgtccgca
    12181 tcactctttc atactcgtca tacttcatcg cgcatttgca gtaaggcgaa atatgacgag
    12241 tatcgggtca tgctttcctg gttcgaattc ttagctgaaa atagaaaaat ggcatggatt
    12301 ttaagcagaa aacaactaaa agttatataa aaaagta
//
LOCUS       DAWDRC010000037        12271 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2681_length_12271_cov_2.564178, whole genome shotgun sequence.
ACCESSION   DAWDRC010000037 DAWDRC010000000
VERSION     DAWDRC010000037.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12271)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12271)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2681_length_12271_cov_2.564178"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5584..5593
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3227 a         3138 c         2876 g         3020 t
ORIGIN      
        1 cctccaccca atcccaacac gggcgctgtc cggatgacgc accggcggag tcccaaatgc
       61 tccgcctgct gtgttgaacg ttcccattgg atggtggtgg tcgccaaaaa acctgaaccg
      121 gcgggactgt cttcggtaca gtcgggagcc gaagcgtttt cgggccagaa tccgtaataa
      181 ccgattgccg aagcctgtat caagactttg ggttttacga tgggcatttg cagcgcgata
      241 acgagcgcct gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg
      301 tggagcacga cgaagacgcg attcgcgccg ctgatcatgt gatcgatatc ggtccgggtg
      361 cgggtgttca cggcggtgaa gtggtcgcgg aaggtccact ggaagcgatt atggcggtgc
      421 ctgagtcgtt gaccgggcag tacatgagcg gtaaacgcaa gattgaggtg ccgaagaaac
      481 gcgtacccgc caatccagaa aaagtgctga aactgacggg cgcacgcggc aacaacctga
      541 aagacgtgac gctgacgctg ccggtaggtc tgtttacctg catcacaggg gtttcaggtt
      601 ccggtaaatc gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg
      661 gtgcgaccat cgccgaaccg gcaccgtatc gcgatattca gggactggag catttcgata
      721 aagtgatcga tatcgaccaa agcccaattg gtcgtactcc gcgttctaac ccggcgacct
      781 ataccggcgt gtttacacct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc
      841 gtggttatac gccaggacgt ttcagcttta acgtccgtgg cggacgctgc gaggcctgtc
      901 agggcgacgg cgtgatcaaa gtggagatgc acttcctgcc ggacatttac gtaccgtgcg
      961 accagtgcaa aggcaaacgc tataaccgtg aaacgctgga gattaagtac aaaggcaaaa
     1021 ccatccacga agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc
     1081 cagctctggc gcgtaagctg caaacgttga tggacgttgg cctgacgtac attcgcctcg
     1141 ggcagtccgc aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc
     1201 tgtcaaaacg cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc
     1261 acttcgccga tattcagcaa ctgctcgacg tactgcataa actgcgcgat cagggcaata
     1321 ccattgtggt aattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc
     1381 tgggaccgga aggcggcagt gggggcggcg aaatcctcgt ctccggtacg ccagaaaccg
     1441 tcgcagagtg cgaagcttcg catacggcgc gcttcctcaa gccgatgctg taatcgttaa
     1501 ggccgctttc agagcggcct tcttctttca gagttgcacc agcaatttac gtttttcttc
     1561 cggcagcaaa ttcaccgcct gctgataaga cgcatccacc agataataga tttgcgaatc
     1621 cggcagcgaa ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggcttgg
     1681 acgcacatcg ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga
     1741 aacagctggg cgattttcta cttctttcac catcgcaaac agcacatctt ccactttgat
     1801 ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg cctggttttg ccatgcaata
     1861 ttgtagcaac tccaaaatgg tcatttttca ctccccttgt agtgtcgcga tgatgcgacg
     1921 cgatccgccg tggatgcgat gttcccccag ccagatgcct tgccaggtgc cggtctgaat
     1981 acgcccttta tgcaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga
     2041 aggcatatcg tctgctccct catagtcatg ctcataattt ccgttatcgg gaacggcgcg
     2101 gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag
     2161 agaggcggag gtatgttgca gcaacagatg cagtaaaccg atgttaacgc gcggcatatc
     2221 agccagctga ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag
     2281 cgtgagcgtc ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg
     2341 cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagtattac
     2401 gctctcccgc tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct
     2461 tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgcca
     2521 acatcgcgtg cggcggtaat atcgttatcg gaatcaccat aaaaaattcg gatattttta
     2581 tcctgcagcc attgcgattt tgtattttga cctggcttat cgcccgcaaa gatcaccgga
     2641 ttcatgttgg tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt
     2701 ttggtcgggc tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca
     2761 atcagctggc gagcgacctc ttttggaatg ctgaattcat cccagccatt gttcattttt
     2821 tcccagaaca caggattttt cagataatct tcgctttctg gcgagaaggt ttttttgccg
     2881 cgccagaaac ccggactgga aaaaagtacc gtatcatcga tatcaaaccc caccgccatt
     2941 ggcggacgtc ctgcgaggct attttcaatt tgtgcaaccg aaacccaatg aattggcgcc
     3001 tgttcagcga gtttggctac gttagtaccc ggattaagcg gtgaaggaga tgaggccagg
     3061 gcaacagcgg aactgtttag agcgaacaat aagcaaacgg cactcaatgc ctgtgtgatc
     3121 ttgcgcatat ttttccctaa atagtcagta agttgaaact tattattgtg cgtttgttga
     3181 aaaaaccatc tgaccataac gccaaccgcg ggggaaagga aggattatct gcggatttgt
     3241 cacataaaaa gagcatgatc acatttggaa tacctgtgcg ataaaccaca cattgccacg
     3301 gttattctgc ttacacattc acacaatggc actcttttgg ttctcgcaaa cattgttcag
     3361 ggaatatact gatgataaat ataaatgcat tcaatggatg atgaatgaaa atatcgtgga
     3421 attatatatt taagaacaaa tggcgatttc acattacaag catttcactt tttcttatca
     3481 tgctcgcggt ttcaatcgct tttttgcact tgcgttttaa taccttgtcc ggtaccgata
     3541 aaatgcggct tgaaatgtat aagtccacat tatattccac catcgagcaa ttttatgttt
     3601 taccctatat gctctcaaca gaccatatca tccgtcaggc ggtaattacg cctgacgata
     3661 tgacgtccag cgaactcaat caacgaattg cacatttcaa tactcaactc aaaaccgcag
     3721 caatatttat tctggatacc caaggtaagg ccatcgcttc tagcaactgg caggaccccg
     3781 gcagctatgt agggcaaaat tatagctatc gcccctatta taaacacgcc atgtctggct
     3841 taaatggacg cttttacggt attggtagca ctacaaatac accgggattc ttcctctcta
     3901 caagtataaa agataaagga aaaattgtcg gtgttgtagt agtaaaaata agtcttaatg
     3961 aaattgaaaa agcatgggcc gaaggtcctg aaaatattat cgtgaatgat gaacatggga
     4021 ttatattttt aagttcaaaa tcgccatggc gaatgcgaac actgcaaccg ttacctgttc
     4081 aggcaaaaca aaaactacaa tctacccgtc aatatagtct cgacaatctt ttaccggcgg
     4141 attattatcc ctgttatacc gtgagcaatt ttactttcct gaaagataaa aaagaacaac
     4201 tctgtttatt cccgcaatat tatacacaac aaatagccat tccagaattt aactggaaaa
     4261 tgacaattat ggtcccctta gataacctgt actggtcatg ggctatttcg ttagtcatta
     4321 cactaattat ttacctgcta tttttgttat ttattaaata ctggagaatg cgatctcatg
     4381 cacaacaatt attaacactg gcgaatgaaa cattagaaaa acaggttaaa gagcgtacat
     4441 ctgccctgga attgatcaat caaaaattaa tacaggagat aaaagagcgc agtcaggctg
     4501 aacaagtatt acaaattacg cgtagtgaac tggcagagtc cagcaaactg gcggcgcttg
     4561 gacagatggc aaccgaaatt gcccatgaac aaaatcaacc gttagccgcc attcacgcac
     4621 ttactgataa cgcgcgcatt atgctgaaaa aagagatgta tccgcatgtt gaacagaatc
     4681 tgaaacatat tatttcagtg attgagcgga tgacgcagct tatatccgag cttaaagcat
     4741 ttgcctcacg ccatcgcgta ccaaaaggct ctgccgatgt catcaaagtg atgtatagcg
     4801 cagtggcgtt acttaatcac agcatggaga aaaataacat tgagcgacga ataaaagccc
     4861 catccatgcc gttatttgtc aattgcgatg agctcggtct tgaacagata ttcagtaatt
     4921 taattagcaa cgccttagat tctatggaag gtagctctta caaacgactg gatatcgcca
     4981 ttcgccaggc aaataacaaa gttattatta ccattaaaga cagcggtggc ggttttgcac
     5041 ctgaagttgt cgatcgcata tttgaaccat tttttaccac taaacgtaga ggaatggggt
     5101 tgggactggc aatagtcagc gaaattgtcc gaaattcgaa cggcgcactc cacgccagta
     5161 atcatcctga aggcggcgca gtaatgacat taacctggcc tgaatgggga gaagaacatg
     5221 aataataaat ccagtgaaat tactatagtt tatattgagg atagtgacga tgtacgtttt
     5281 gcctgtgaac aaacgctcac gctggctggt tatcgtgtta tttcctgttg cgatgcagaa
     5341 cacagtattt cccttattca aagtcaagcc aacattatta tactgaccga tgtcaggtta
     5401 cctggaatat caggactaga gctcctcagt tacatcaacg aaatggatag caaaattccg
     5461 gttattttaa ttaccggaca cggtgacgtg gaaatggcgg tggatgccat gcgtaatggt
     5521 gcattcgatt tcattgagaa accaagtagc tcagataaat tattgagtat tattgccatg
     5581 gagnnnnnnn nnncagagcg gtagaaaaac ggcgattggt tttagaaaat cagcaattac
     5641 tggcgaatct gcaacaggaa aatggcccgg tgctgattgg tcgtagtccg cagatgcaac
     5701 aactgcgtaa aatgatcctt aatgtcgcag ataccggtgc cgatgtcctt atttatggtg
     5761 aaacaggttg tggtaaagaa gtcgtcgcca gaatgctgca tcactggagt acccgccgtc
     5821 aggggcagtt tgttgcctta aactgtgcag gcttaccaga aacgttgttt gaaagcgaaa
     5881 tatttggtca tgaggcgggc gcatttaccg gtgcggttaa aaagcgtatt ggaaaaattg
     5941 aacatgcaaa tggtggtacg ttatttctcg atgaaataga gggaatgcca tcaggaatgc
     6001 aggtaaaact gctacgtgta ttgcaggaaa gaaccattga acgattaggt gccaatcaat
     6061 taattccggt gaattgccgt gttattgcag cgaccaagga agatttactg cgtcgcagcg
     6121 aagaacatct gtttcgtctc gatctctatt atcgtctgaa tgtggttagc ctgaatattc
     6181 cacctctacg ccagcgacga gaagatatcc ctgaactgtt ttactggttc gccagtcagg
     6241 cggcacaaaa atataatcgt cctctgcctg atatttctcc catgttactg gcctggttgc
     6301 agtcacaaag ttggcctggc aatgtacgtg aacttaaaca caatgcagag cgttttgttc
     6361 tcggtttact gacccaccac cagccagtgc caatgactca gcaagaagag agtggtctta
     6421 ccgcctgcat tgatgctttc gagaaaaagt taatcgaaga tatgctgcga caaacggaag
     6481 gccaggtcag tttgacagcg cggctattac agctgccgcg caagacttta tatgacaaac
     6541 tcaacaaaca tcagattcaa ccgcaagttt atcgaccaga atcatccagc taatgctgat
     6601 tcctgaggat gtaaccccaa atcgatacct ttgcgggcgt aatgttcaac tacccaggac
     6661 cagaatgata ccgggatcgt aatcatcttc gcattgcgtt cccggttggc agcttctggc
     6721 ataccggggt aaatcaccgg attgccgcca ggttgaggct gcgtagctaa cagataatcc
     6781 gcaaagaact gccctttctc ccctgccaga tcggtgaatt gatcaggatc gataatcaac
     6841 gcaaaaagat gattatgcgc agatccatcc gtctgatgat cttcggcgat cgtgtcacca
     6901 ccagataaga tccccgccat catttcaatc atcagtgcca ggccaaatcc tttatgctca
     6961 ccaaatggca gtaacgcccc tggaggattg ctgaataaca ccccaggatc attgcttgga
     7021 ttcccctgtg catcaatcaa ttgtcccggc gcggcctgct tcccggtgga cgacattacc
     7081 cgtgctttat ttagcgccat gctactggtg gcaaaatcaa ccagaatcgg cggacgccca
     7141 tcactgaacg gccaggcaaa gcagaaaggg ttagtggtca tacgcggttc cgcaccgccc
     7201 cacggggcga ccagtgcagg acgcccggtg acgttactca tggccagcat cgccaggcct
     7261 tgatcagcaa ccatttgccc gtaatgcccc atacgcccta aatgatgcgc atggcacaac
     7321 gttaacaggc aaaaaccgcg gtgttttgct tgttcgatgg cagcatttac cgccactttt
     7381 ccggcgtgtt gaccaaagcc attgtgggca tgaaaacgtt gtaaattacc ttcacttgtt
     7441 aagcattccg ggcgggcatt cgcggttaca tcgccacgag cgatattttc cagatatttc
     7501 ggtagcagcg tgacgccgtg gctggcaaag ccataacgtt ccgcttcaat catatgttcc
     7561 gccacatcct gagcgatatc ggcatcacag ccattaagtt ccagcagccc ggcagctaac
     7621 caacgcactt tttcaacagc aatttgtaat gacatcgttg gtttcctcac attagaccta
     7681 aaattttcca ccaggtggcg gcaaacagta gcaacaacag gtagccgatt accgtcaggt
     7741 acagtcccac acgggtaaac tggcgtggcg tgaaggtatc cgtacccata cacaccatat
     7801 tttgtggcgc gttaatcggc aggatgaagc caaaactgac actgaaggcg agcagaatgg
     7861 tcatgccgac cgggttaacg ccgagttctg gcggcagact gcttaacagg ctaatcagga
     7921 tgggcagcaa tgcggcagtt agcgcagtgg cactggcaaa ccccaggtga ataattatca
     7981 agaaagcggc gagaatcgcg aagatggcta atgatggcaa gccatcgaga ccaaaacctt
     8041 tcaccacata gttcgccatc catgaagctg cctgcgtatc aagcagcgtg gaccccaggc
     8101 tgataccaat accaaacata agcaacgtgc cccactgcac acgtttttcg acctctttcc
     8161 agctcattac accgatgcct ggcagcaaca taatcgccag tccggcaagg gtgacagagg
     8221 tggtatcaat actgtgtaat tttccgccag tagaccaaaa gagcagtaac aatagcgaaa
     8281 taccgatcaa acgtttttct ttaccagtgg ttggccctaa ctctgcaagt gcttttttaa
     8341 tggcctcgct ccccccttca ccagcttccg tttctggcgg caacaattta cgtgcgagga
     8401 aatagaggat cgccgacatg gtgaggctcc agggcgcgcc cgctaacagc cagtcaagcc
     8461 agctgacaga atgcccggcg ccgaaagttt tattgataaa accgatagaa agcaggtttt
     8521 gcgccgccga agtttgaata ccaacgttcc agatactggt cgcctgggcg ataacaatca
     8581 tcattgacgc cgctaaacgt gaatgtttat ccaccttaaa tgccgcaatg acccccatca
     8641 taatcggcac cacgcaagcc gtacgggcag tggcactggg tacaaccagg ctcaggacga
     8701 tggtcaccac aatggcaccg ataataatgc tgcggctgct ggcacctatt ttcgacatag
     8761 taaacagcgc aatgcgttta tccagctcgg taatggtcat tgccgccgcg ataaacattg
     8821 ctgctgccac cagcgccaga gcagaattag aaaaacccga cagagtcatt ttcagcgctt
     8881 tcgcggtgcc aaggatcgtg tccgggtgat tcatatccgg ggcaaaacca accataaaga
     8941 taatcagtgc ggaaataaca attgcactgg cggtgtaatc catcgcttcg ctgatccaga
     9001 caataatggc aaacaccaga atggcgatca tattttgtcc agcgacgggc aaggtatcgc
     9061 ccagtggcag aagggtaata ataattccgg caatgatcgc caaccacagg ccgggattag
     9121 ggataattcg acgcttttgt ggtgcgccat gggatattga tgacatattc acctcattgt
     9181 gaaaaataaa aaacctgcct ggctattgca ggagctatgc catctttaat tttttaataa
     9241 cgctttgtta tttaatgagt tagctattta tcctttttat tttatgcgcg gttttccgca
     9301 cagttattaa aagatgattc ccttaccaca cagataaaaa aagggagcga tatgctccct
     9361 tgatattcat ttatggatta cttaataaca ttccataacg tttcgccaag caacgccggg
     9421 tttcttgtcc atgccatgcc tgctttatcg aacgcacgga atttatcctc tgcactgccg
     9481 ctaccgccag aaataatggc tccggcgtgc cccatgcgtt ttccttttgg tgcagtaaca
     9541 ccggcaatat aacctacgac cggtttattc atttcatgac gaataaattc cgcagcccgt
     9601 tcttccgcat taccaccaat ttcaccgatc ataattaccg cgtctgtttg cggatcattt
     9661 tcgaataaac gcagcgcatc aataaagtta gtccccggaa ccggatcgcc gccaatacca
     9721 atacaggtgg attgcccaag tcccagcgcc gtggtttgcg ctacagcttc gtaggttaat
     9781 gtaccggagc gggaaacaat cccaatacgc cctgggcggt ggatttcacc aggcataatc
     9841 ccgattttgc actcacccgg cgtgataact cctgggcagt ttggcccaat cagccgcaca
     9901 tccgggtggc agtcaagcag ccgacgaact ttcaccatat ccagtgtcgg tacaccttca
     9961 gtgatcacca caaccaattt gacgccagct tcaatagcct caacaacaga atccagcaca
    10021 aacggtgcag gcacataaat cacgctggca tcggcctcag tttgtcgcat cgcttctttc
    10081 attgaatcaa aaaccgggcg atccagatgc cgttgcccgc ccttacctgg cgtcactcca
    10141 cccacaatct gcgtgccata cgcaatagcc tgctctgtat gaaatgtgcc gtttttgccg
    10201 gtaatgccct gcaccagtac ccgcgtgtgt ttgttgatca aaacgctcat cttaacctcc
    10261 tgttaattca gtaacgcaat gatgcgttta gcggcatcat ccagagaatt gacggcttcc
    10321 accgtcaggc cactttctgc cagtaaccgc tgcccttcgg cagcgttatt accggagaga
    10381 cgcaccacga caggcagggt gatacgggcc tcattcagag catgaataat ggctctggcg
    10441 atcatgtcgc aacggacaat cccaccaaaa atattaacca gaatggcttt cactttgctg
    10501 tcggaaacaa tcagccgaaa ggcttcactg acgcgctcct gggttgcgcc accgccaaca
    10561 tccaggaagt ttgcgggttg ttcaccgtat agcttgatga tgtccatggt tgccatagcc
    10621 agccctgcac cattgaccat gcagccgata tttccgtcca gcgagacata attaagatca
    10681 agcttcgctg cctggctttc gcgtggatct tcctgggttt catcacgtag cacctggagt
    10741 tccgggtgac gatacagcgc gttatcatcc agcgatactt tcgcgtctgc acacataaat
    10801 tcgcccgttt cccgcaacac cagagggtta atttccagca gggcgaaatc cagttcgtta
    10861 aaggctttcc acgcctgatt aaccaggcga ctaaaggtag caaatagccc gtgttccagt
    10921 tgcagaacag caaacatttc gcggatatgg caaggctgca cgcctgtcag tggatcaatg
    10981 ctgacactgc tgattttctc cggtgtctca tgggcgactt tttcaatttc cacaccacct
    11041 tccgggctga caataaacgt gacccgctgg ctttcacgat ccaccaccat gccaaaatag
    11101 agttcctggc gtaccggata gatgttttcg cacagcaaaa tactgctgac atactgccct
    11161 tctggcccgg tctgataagt caccagttgc gatcccaaca tttgctgtac aaaagcctgt
    11221 gcttccggca gctgcttaag cacttttacg ccgccagctt taccgcgccc accagcatgt
    11281 acctgcgctt taagtaccgc gcctttactc gggcaagcaa tatgttgcca ggcatctgcc
    11341 agttgcgaaa tctgttggat ggctatctct tttggacacg gcatccccat gccagccagt
    11401 aacgatttgg cctgatactc atgtaaattc atatattccc tcagtggtat gtgttatccg
    11461 tgtaatgctt tattattcaa ggccattgct gcttcgtgga tgacttcgct taatgtcgga
    11521 tgcgcatgga tcgcacaggc aatatcctcg ccagaggcgc taaaagtcat cgccagcgca
    11581 atttcattga tcagctccga tgcctgcggg ccaacaatcg ctccgccaag tacgcggtca
    11641 gtgtgtttat cgctgtataa cgtgcaacgc ccaccttcct ggcctaatgc taaggcgcga
    11701 ccgttaccgg caaacagaga atttcctttg ttaaagactc tgccagcggc tttcagtgag
    11761 gcctcgtttt cccctaccca cgcaacttcc ggttgggtgt aaatcaccga agggatcagc
    11821 gcaaaattaa tcggctctac cgccagtccg gcaatttgat cagcaaccac cacaccttca
    11881 gccatcgctt tatgcgccag catcggccct cgcacgacat caccaattgc ccacagcccg
    11941 gctttcccgg tacggcataa gttatcgacg gcaataccgc cacggttatc ggcttcaaga
    12001 ccgagttgca cgagatcgac accagaaagt cgcggtacac gtccaatggc aagaattaat
    12061 ttatcgaagc ggctttcttc tcgcttctca ccctgccgcc agcgcacatg cacaccgtca
    12121 tcccgttgtt caatagcctc aatctctact gccagttgca ttttcatccc actggcaatc
    12181 attgctttac ggacttcgtt cgacaggcga gcttccagtg caggcaagaa cgttggcgcc
    12241 atctccagca acgtgacgtc tgaacccacc c
//
LOCUS       DAWDRC010000038        12044 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2732_length_12044_cov_2.673367, whole genome shotgun sequence.
ACCESSION   DAWDRC010000038 DAWDRC010000000
VERSION     DAWDRC010000038.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2732_length_12044_cov_2.673367"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    219..228
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4085..4094
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5637..5646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11541..11550
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2861 a         3018 c         3197 g         2928 t
ORIGIN      
        1 gcacacctga aagcctgtaa cgttctcgat ttcgatgatc tgattttatt gccaacgttg
       61 ctgctgcaac gcaatgaaga agtccgcgag cgctggcaga acaaaattcg ctacctgctg
      121 gtggatgagt atcaggacac caacaccagc cagtatgaac tggtgaaact gctggtgggc
      181 agccgcgcgc gctttaccgt ggtgggcgat gacgaccann nnnnnnnncc tggcgcggcg
      241 cacgtccgca gaatctggtg ctgctgagcc aggattttcc ggcgctgaag gtgattaagc
      301 ttgagcagaa ctaccgctct tccggacgta ttttgaaagc ggcgaacatc ctgatcgcca
      361 ataacccgca cgtctttgaa aagcgtctgt tctccgaact gggttacggc acagagctaa
      421 aagttttaag cgcgaataac gaggaacatg aagccgagcg cgtcaccggc gagctgatcg
      481 cccatcactt cgtcaataaa acgcagtaca aagattacgc cattctttat cgcggcaacc
      541 atcagtcgcg ggtatttgaa aaattcctga tgcaaaaccg catcccgtac aaaatttctg
      601 gtggtacgtc gtttttctct cgtcctgaaa tcaaggactt gctggcttat ctgcgcgtgc
      661 tgactaaccc ggacgatgac agcgcatttc tgcgtatcgt taacacgccg aagcgagaga
      721 ttggcccggc gacgctgaaa aagctaggtg aatgggcgat gacgcgcaat aaaagcatgt
      781 ttaccgccag ctttgatatg ggcctgagtc agacgcttag cggacgtggt tatgaagcat
      841 tgacccgctt cactcactgg ctggcagaaa tccagcgtct ggcggagcgg gagccgattg
      901 ccgcggtgcg cgatctgatc cacggcatgg attatgaatc ctggctgtac gaaacatcgc
      961 ccagcccgaa agctgccgaa atgcgcatga agaacgtcaa ccaactgttt agctggatga
     1021 cggagatgct ggaaggcagc gaactggatg agccgatgac gctcacccag gtggtgacgc
     1081 gctttactct gcgcgacatg atggagcgcg gcgagagcga ggaagagctg gatcaggtgc
     1141 aactgatgac tttgcacgcg tcaaaaggac tggagtttcc ctatgtctac atggttggta
     1201 tggaagaagg atttttgccc caccagagca gtatcgatga agataatatc gatgaggagc
     1261 ggcggctggc ctatgtcggc attacccgcg cccagaagga attgaccttt acgctgtgta
     1321 aagaacgccg tcagtacggc gaactggtgc gcccggagcc gagccgcttc ctgctggagc
     1381 tgccgcagga tgatttgatt tgggaacagg agcgcaaagt ggtcagcgcc gaagaacgga
     1441 tgcagaaagg gcaaagccat ctggcgaatc tgaaagcgat gatggcggca aaacgaggga
     1501 aataatcaag gccggaaaag atgtgtcagc atcgcatccg gcacttactc attaatgcac
     1561 ttccagcggc cagtggacat agctctgcca ctggctttcc tgatcaataa tctctttacc
     1621 cagcggatgt tgggttagcc aaccttgcgg aagcgtcaag gtcaacagtt catggttagc
     1681 ctgtaatgtc atctctggca cgagatcgtc acggcgacgg ctggcaaaaa tgatagccag
     1741 gcggagtaaa cggcagagtt gttctgcgac gcgcggtggt acggcatttt gctgatgcag
     1801 cgatgagaga tcgaccggat tagtctggtt gagcagtagc gtcgccagca gttttttctg
     1861 tgcgggggta aaaccgggaa gatccagatt acgtaccaga taagcagcgt gttgcggcgc
     1921 ttgtttgaag tcaacgctca ggccgatttc atgaagctga caggcgctga tgagcaaatc
     1981 gcggcttatt gcttcaagat gccattcatt ttccacctga tcgtagaagt tagccgcaac
     2041 tttggctacg cgctgtgcct gatcaatatc gatcataaag cggcgctgaa tattacgcag
     2101 cgtacggctg cgaatatcct gctcgacggt aagatgcaac ataccgtaga ccaggccttc
     2161 acgcagcgca ccgcccgcca gggtcataca ctgaatattc agttcggtaa aaatggctat
     2221 caggatcgcc agaccactcg ggaacactaa cgcacgttcc agcgtcagcc cgtcaatctc
     2281 tagctcttcc agccgaccgc aatgaatggc gcgttgtttc agttgctgca acttttccag
     2341 ggtaatgcgt tcatccatcc cctgtgccat catgatttcc tgtaacgcct gcacggtgcc
     2401 ggaagcgcca acgcacactt tccagccgtg ataccgtaat tcatcggcaa ccggacgtaa
     2461 cacttcgcgt gccgcttttt ctgcagcatc aaaattttcc tgccccagat tacgatcggc
     2521 aaaatagcgt tccagccagg taacgcagcc catcgacagg ctgaacaaag aggtggtttg
     2581 tgcacccgtg ccggttacca gttcagtact ggctccgccg atatccacca ccaggcgctg
     2641 atcggcaccg ccagtggtgt gagcaacgcc ctgataaatc agacgcgctt cctcttcacc
     2701 gctgatcacc tgtaccggac aaccgaggat ttcctgcgct ttggcaataa aatcacccgc
     2761 attgacggca agtcgtaatg tcgccgtagc gacaacgcga atttgcgagg gagggatatc
     2821 ttgcagacgt tcaacaaaca ggcgcagaca ttgccaaccg cgctccattg cttcattaga
     2881 cagggcattt tcgctattca ggccggcagc cagacgcact ttgcgtttta ttcgcgtcag
     2941 cgtctggatg cttccagcca cctcgcgcac aaccagcata tgaaaactat tcgaaccgag
     3001 atcaatggct gcatacagcg acgaggtgga acccatactc ttcatccttc aatctgtctc
     3061 tgcgttggct gcattccttc acctgaatca cttacgacag taagcgcatc gggattcatt
     3121 ctcttgccat cttgatacag cttgaatgat ttggagtatg acattttttc tttaacctga
     3181 acgacgacga ttacgcggag cgccagtacg acgcggacca ttgcctgtgc gcgggcgcgt
     3241 gaggcgcagc ggttttggca gatcggtcat caatgcgtcc ggattgtatt tacttaccgg
     3301 aattgagtga ccaatatagg tctcaatagc aggcaaattc aatgcatact cttcacacgc
     3361 caggctaata gagtgaccgc ttgcgcctgc gcgacctgtt cgaccaatac ggtgaacgta
     3421 atcttcacag tcatcgggta aatcgtagtt aaagacgtgt gtcacagcag gaatatgcaa
     3481 accacgcgcg gcaacgtcgg tggcaaccag aatatccaga tccccacggg taaattcatc
     3541 aagaatacgc agacgttttt tctgcgcgac atcgcctgtc aataaaccga cacgatgacc
     3601 atctgccgcc aggtggcccc agatctcttc acaacggtgt ttggtgttgg cgaaaataat
     3661 cgctctgtct ggccactctt cttcgatcag cgtttgcagc aaacgcattt tttcttcgtt
     3721 agaagggtag aaaagctctt ctttaatacg gtggcccgtt ttctgttccg gttccacttc
     3781 aatatattcg gcattgttca tctgctcgaa cgccagttca cgtacccggt acgaaagcgt
     3841 ggcggagaac agcatgttga ggcgctggtt tgcaggcggc atacggcgga acagccagcg
     3901 gatatcttta ataaagccca gatcgtacat gcgatcggct tcgtccagta ccaccacctg
     3961 aatggcaccg aggttaatgt ggttctgctt ggcgtagtca attaaacgac ccgtggtgcc
     4021 aatcagaatg tcaacgccgc tttccagcac tttcagctgt ttgtcgtagc catcaccacc
     4081 gtaannnnnn nnnncggttc cgcgtcggcg tggatctgca cggcaagttc acgcgtcggt
     4141 gccataatta aggcacgcgg ctgattcacc ttgcgatcgg caatcgcggg atgagagaga
     4201 agataatgaa acgttgacgt aagaaacgcc atcgttttcc cggtaccggt ttgcgcctgc
     4261 ccggctacgt cacgacccgc cagcgtcagc ggaagggcca gtgcctgaat gggcgtacag
     4321 ttatgaaacc cttttttttc aagggcttct acaaccttcg gatgcagggc gaagtcggaa
     4381 aacttctgtt ctgttaaatg tgttttgctc atagtgtggt agaatatcag cttactattg
     4441 ctttacgaaa gcgtatccgg tgaaataaag tcaaccttta gttggttaat gttacaccaa
     4501 caacgaaacc aacacgccag gcttattcct gtggagttat atatgagcga taaaattatt
     4561 cacctgactg acgacagttt tgacacggat gtactcaaag cggacggggc gatcctcgtc
     4621 gatttctggg cagagtggtg cggtccgtgc aaaatgatcg ccccgattct ggatgaaatc
     4681 gctgacgaat atcagggcaa actgaccgtt gcaaaactga acatcgatca aaaccctggc
     4741 actgcgccga aatatggcat ccgtggtatc ccgactctgc tgctgttcaa aaacggtgaa
     4801 gtggcggcaa ccaaagtggg cgcactgtct aaaggtcagt tgaaagagtt cctcgacgct
     4861 aacctggcgt aagggaattt catgttcggg tgccccgtcg ctaaaaactg gacgcccggc
     4921 gtgagtcatg ctaacttagt gttgacttcg tattaaacat accttattaa gtttgaatct
     4981 tgtaatttcc aacgcttccc gttttatctt aaatgcgaag tgaacagatt tctggctcgt
     5041 cactcaatcc gtcttgtcgt ttcagttctg cgtactctcc tgagaccagg cagcgaaaag
     5101 acatgagtcg atgaccgtaa acaggcatgg atgatcctgc cataccattc acaacattaa
     5161 gttcgagatt taccccaagt ttaagaactc acaccactat gaatcttacc gaattaaaga
     5221 atacgccggt ttctgagctg atcactctcg gcgaaaatat ggggctggaa aacctggctc
     5281 gtatgcgtaa gcaggacatt atttttgcca tcctgaagca gcacgcaaag agtggcgaag
     5341 atatctttgg tgatggcgta ctggagatat tgcaggatgg atttggtttc ctccgttccg
     5401 cagacagctc ctacctcgcc ggtcctgatg acatctacgt ttcccctagc caaatccgcc
     5461 gtttcaacct ccgcactggt gataccatct ctggtaagat tcgcccgccg aaagaaggtg
     5521 aacgctattt tgcgctgctg aaagttaacg aagttaactt cgacaaacct gaaaacgccc
     5581 gcaacaaaat cctctttgag aacttaaccc cgctgcacgc aaactctcgt ctgcgtnnnn
     5641 nnnnnngcgt atggaacgtg gtaacggttc tacggaagat ttaaccgctc gcgtactgga
     5701 tctggcatca cctatcggtc gtggtcagcg tggtctgatt gtggcaccgc cgaaagccgg
     5761 taaaaccatg ctgctgcaga acattgctca gagcattgct tacaaccacc cggattgtgt
     5821 gctgatggtt ctgctgatcg acgaacgtcc ggaagaagta accgagatgc agcgactggt
     5881 aaaaggtgaa gttgttgctt ctacctttga cgaacccgca tctcgccacg ttcaggttgc
     5941 ggaaatggtg atcgagaagg cgaaacgcct ggttgagcac aagaaagacg ttatcattct
     6001 gctcgactcc atcactcgtc tggcgcgcgc ttacaacacc gttgttccgg cgtcaggtaa
     6061 agtgttgacc ggtggtgtgg acgccaacgc cctgcatcgt ccgaaacgct tcttcggtgc
     6121 ggcgcgtaac gtggaagagg gcggcagcct gaccattatc gcgacggcgc ttatcgatac
     6181 cggttctaaa atggacgaag ttatctacga agagtttaaa ggtacaggca acatggaact
     6241 gcacctctct cgtaagatcg ctgaaaaacg cgtcttcccg gctatcgact acaaccgttc
     6301 cggtacccgt aaagaagagc tgctcacgac tcaggaagaa ctgcagaaaa tgtggatcct
     6361 gcgcaaaatc attcacccga tgggcgaaat cgatgcaatg gaattcctca ttaataaact
     6421 ggcaatgacc aagaccaatg acgatttctt cgaaatgatg aaacgctcat aaatttgtct
     6481 tatgccaaaa acgccacgtg tttacgtggc gttttgcttt tatatctgta atcttaatgc
     6541 cgcgctggcg atgttaggaa aattcctgga atttgctggt gtgttatgca atttgcatat
     6601 caagtagtta attttcgcat agtgctggta ggttcggaac ggactttcct ttctgaataa
     6661 aggtcttcgt ggttatactt ctgctaataa ttttctctga gagcatgcat tgtgaattta
     6721 ctgacagtga gtactgatct catcagtatt tttttattca cgacactgtt tctgtttttt
     6781 gcccgtaagg tggcaaaaaa agtcggttta gtggataaac caaacttccg caaacgtcac
     6841 cagggattga tacctctcgt tggggggatt tcggtttacg cagggatttg cttcacgttc
     6901 ggaattgtcg attactatat tccgcatgca tctctctatc tcgcttgtgc tggtgtgctt
     6961 gttttcattg gcgcgctgga tgaccgtttt gatatcagcg taaaaatccg tgccaccata
     7021 caggccgctg ttggcattgt tatgatggtg ttcggcaaac tttatctcag tagcctgggt
     7081 tatatctttg gctcctggga gatggtgctc ggaccgtttg gttacttcct gacgctattt
     7141 gccgtctggg cggccattaa tgcgttcaac atggttgatg gcattgatgg cttgctgggc
     7201 gggttgtcct gcgtctcgtt tgcagcaatc ggtatgattt tgtggttcga cgggcaaacc
     7261 agtctcgcaa tctggtgctt tgcgatgatc gccgccatcc tgccatacat catgcttaac
     7321 cttggtatcc tgggtcgccg ctacaaagtc tttatggggg atgcgggcag tacgctgatt
     7381 ggttttaccg ttatctggat cctgctcgaa acgacccagg gcaaaactca tcccatcagc
     7441 ccggttaccg ctttgtggat aatcgccatt ccgctaatgg atatggtggc gattatgtac
     7501 cgtcgcctgc gtaaaggcat gagcccattc tctccagacc gtcagcatat tcaccatttg
     7561 atcatgcgtg ccgggtttac ttcccgccag gcgtttgtgc tgattaccct tgccgcagca
     7621 ctgctcgctt ccattggcgt gctggcagaa tattctcatt ttgtcccgga gtgggtcatg
     7681 ctggtgctct ttttgctagc attcttcctc tatggatatt gcattaagcg tgcctggaaa
     7741 gttgctcgct ttattaagcg cgtaaaacgc agactgcgta gaaatcgtgg tggcagcccc
     7801 aatttaacca aataaatgag gatgtgatga cacaaccaat gcctgggaaa ccggccgaag
     7861 acgctgaaaa tgaactggat attcgtgggt tgtttcgtac cttgtgggct gggaagctgt
     7921 ggattattgg catggggctg gcgtttgcgt taatcgcgct ggcgtatact ttttttgctc
     7981 gccaggagtg gagctcgaca gcgattaccg atcgtccaac ggtgaatatg ctggggggat
     8041 attactcaca gcagcaattt ttgcgtaacc tggatgtccg ttcaaacatg gcttctgccg
     8101 accaaccatc ggtcatggac gaagcctata aagagtttgt tatgcaactg gcctcgtggg
     8161 atacccgcag agagttctgg ctgcaaaccg actattacaa acagcggatg gtgggcaata
     8221 gcaaagccga tgcggcgttg ctggatgaaa tgatcaacaa catcgtgttt attcccggtg
     8281 acttcacccg cgcggtcaat gacagcgtga agctgattgc cgaaactgcg cctgacgcta
     8341 ataacctgtt acgtcagtat gttgcttttg ccagccagcg tgcagccagc catctgaatg
     8401 atgagctgaa aggcgcatgg gcggcacgca ccatccagat gaaagctcag gtgaagcgtc
     8461 aggaagaggt ggcgaaagcc atctacgatc gccggatgaa cagtattgaa caggcgctga
     8521 aaattgctga gcagcataat atttcgcgca gtgcgacaga tgtgcctgcc gaggaattac
     8581 ctgattcaga aatgttcctg cttgggcgtc caatgctcca ggctcgactg gaaaatttac
     8641 aggccgtcgg tccggccttt gatctcgact atgatcagaa tcgggccatg ttaaacaccc
     8701 tgaatgttgg tccaaccctg gatccgcgtt ttcagaccta tcgctatttg cgtacgccgg
     8761 aagaaccggt aaaacgcgat agcccacgtc gtgccttcct gatgattatg tggggcattg
     8821 tcgggggact gatcggggct ggtgtcgcat taacccgccg ttgctcgaaa tagcaacact
     8881 gctgcggtga gcgcaaaggc gctcgccgct tattcgaaga gaatcgatgt gaaagtactg
     8941 actgtatttg gtacgcgccc ggaagccatc aagatggcac cgttggtgca tgcgttggca
     9001 aaagctcctt tttttgaggc taaagtttgc gtcactgcgc agcatcggga gatgctcgat
     9061 caggtgctga aactcttttc cattgtacct gactacgatc tcaacataat gcagccagga
     9121 caaggcctta cagagataac ctgtcggatt ctggaagggc taaaacctat tcttgccgag
     9181 ttcaaaccag atgtcgtgct ggttcacggc gatactacga cgacgctggc aaccagcctg
     9241 gcggcgtttt atcagcgtat tcctgttggt cacgttgagg ctggtctgcg cacgggcgat
     9301 ctctattcgc cgtggccgga agaggctaac cgtacattga ccgggcatct ggcgatgtat
     9361 cacttctctc caaccgaaac ttcccggcaa aacttgctgc gtgaaaacgt tgcggatagc
     9421 cgaatcttca ttactggtaa tacagtcatt gatgcactgt tatgggtgcg tgaccaggtg
     9481 atgagcagcg atacgctgcg ttcagaactg gcggcaaatt acccgtttat cgaccccgat
     9541 aaaaagatga ttctggtgac cggtcacagg cgtgagagct tcggtcgtgg ctttgaagaa
     9601 atctgccatg cgctggcaga catcgccacc acgcaccagg acatccagat tgtctatccg
     9661 gtgcatctca acccgaacgt cagagagccg gtcaatcgca ttctggggca tgtgaaaaat
     9721 gtcattctga tcgatcccca ggagtattta ccgtttgtct ggctgatgaa ccacgcctgg
     9781 ctgattttga ccgactcagg cggcattcag gaagaagcgc cttcgctggg gaaaccggtg
     9841 ctggtgatgc gcgataccac tgagcgtccg gaagcggtga cggcgggtac ggtgcgtctg
     9901 gtcggcacgg ataagcagcg aattgtcgag gaagtgacgc gtcttttaaa agacgaaaac
     9961 gaatatcaag ctatgagccg cgcccataac ccgtatggtg atggtcaggc atgctctcgc
    10021 attctggaag cgttaaaaaa taatcggata tcactatgag ttttgcgacc atttctgtta
    10081 tcggactggg ttacatcggg ctgcctacag cagctgcgtt tgcctcaagg caaaaacagg
    10141 tgattggtgt cgatatcaac caacacgcgg ttgataccat caatcgtggc gaaatccata
    10201 tcgtcgagcc tgatttggcg agcgtagtaa aaactgccgt agaaggcggt tttttacgag
    10261 cgagcacgac gccggttgaa gcggatgcct ggctgattgc tgtacccacg ccgtttaaag
    10321 gcgatcatga gccagatatg acctacgttg aatcggctgc tcgctccatt gcgccagtgc
    10381 tgaaaaaagg cgcgttggtg atccttgaat ccacctcgcc ggtggggtca accgagaaga
    10441 tggcagaatg gttagcagag atgcgtccag atctcacttt cccacagcag gtgggcgagt
    10501 tggcggacgt caacattgct tactgcccgg aacgcgtatt accgggccag gttatggtcg
    10561 agctgattaa aaacgatcgc gtgattggtg gtatgacgcc ggtttgttcg gcccgcgcca
    10621 gcgaactgta caaaattttc ctcgaaggtg agtgtgtcgt aactaactcg cggacggcgg
    10681 aaatgtgtaa gctcaccgaa aacagctttc gcgatgtgaa tatcgctttt gctaatgaat
    10741 tgtcgctgat ttgtgccgat caggggatta acgtctggga actgattcgc ctggcgaatc
    10801 gtcaccctcg cgtcaatatt cttcagccag gccctggcgt gggcggtcac tgcatcgctg
    10861 ttgatccgtg gtttatcgtg gcacagaacc cccagcaggc gcggcttatc cgtaccgcgc
    10921 gcgaagtgaa cgatcacaaa ccgttctggg ttatcgatca ggtgaaagcg gcggtggctg
    10981 attgtctggc tgctaccgat aaacgcgcca gtgaactgaa aatcgcctgc tttggtctgg
    11041 cgtttaaacc gaatattgat gacctgcgcg aaagtccggc aatggaaatc gctgaactga
    11101 tcgcccagtg gcacagcggc gaaacgctgg ttgttgagcc taacatccac gagttgccga
    11161 agaaactgac cgggctttgt actctggcgc agcttgacga ggcgctggca acggcagatg
    11221 tgctggtgat gctggtcgat catagtcagt tcaaagttat caatggcgac aacgtccatc
    11281 agcagtatgt cgtcgatgcc aaaggagtct ggcgctgatg agaaagattc tgataacagg
    11341 tggtgccggg tttattggct cggcgctggt gcgttatatc atcaacgaaa cgagcgacgc
    11401 ggtggtggtg gtcgataagc tgacctacgc cggaaacctg atgtcgctgg caccggtcgc
    11461 gcaaagcgag cgctttgcct ttgagaaagt tgatatctgc gatcgggcag aactggcgcg
    11521 cgtattcact gagtatcagc nnnnnnnnnn gtgtcatgca tctggcagcc gaaagccatg
    11581 ttgaccgttc tattgacggc ccggcagcgt ttattgaaac caacattgtc gggacttata
    11641 cactgcttga agcggcgcgg gcttactgga ctgcgctgac ggaagataaa aaatcagcgt
    11701 tccgttttca tcatatctcc accgacgaag tatatggtga cctgcactcg acggatgatt
    11761 tcttcaccga aaccacgccg tatgcgccga gtagccctta ttccgcgtca aaagccagca
    11821 gcgaccatct ggtgcgcgcc tggctgcgga cctatggtct gccgacgctt atcaccaact
    11881 gctcgaataa ctacggtcct taccactttc cggaaaaact gatcccgctg atgatcctca
    11941 acgcgctggc gggtaaaccg ctgccggtat atggcaacgg gcagcaaatc cgtgactggc
    12001 tgtatgttga agatctggcc cgtgcgctgt atggcgtggc gacc
//
LOCUS       DAWDRC010000039        11961 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2767_length_11961_cov_2.147909, whole genome shotgun sequence.
ACCESSION   DAWDRC010000039 DAWDRC010000000
VERSION     DAWDRC010000039.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11961)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2767_length_11961_cov_2.147909"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1774..1783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3271..3280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6634..6643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7379..7388
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9750..9759
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11009..11018
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11548..11557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3152 a         2512 c         3215 g         3012 t
ORIGIN      
        1 taattgcaat aacgcaatgg cagatgtcat attgtcacca gactggcgtc cgggtacgaa
       61 taactcgggt gttggggctg caacagtaag tggtaaaacc gaatacatca ctggtccaaa
      121 tgtcgtccag tctggtgggt caggtcttat ctggatgact gtagaacagg caattttaaa
      181 tggctacacc actggagata atttatccgg attgatttac gtcaataccg gagaaaaaac
      241 aaaaaccatt acagtgaagg atgaggttac tggcgcttct caaacacttc aagtgtttga
      301 tactgactct ttctcacaac gcgatgcggg gactggggga aatgaaacca ttcctggttt
      361 tagtggcact gcggattttt tcaatgcgac acgttttgta acagccaata atggcggtac
      421 agctattttg gatgtgggtt caccagcaat cggtaatttt tttaaaaata cacagcttgc
      481 tgtagctgat ggagaaggtt cctctgttgt atggaactcg gtcaatgatt tttattttca
      541 gcctggtgca accatgcagg ggggcggcgt tactcaaaaa atcattgact caatgaaata
      601 tgctggaaca attactgatt gggcgggaaa agtacaccat attaactctc ttgatgattt
      661 aaagcaatat aatcaatatt tgataaagtc actagaggac aaaacgcttt cttataagca
      721 gtatgatgct gaatttaata aggcccttat tgtcaccaag cataattaca atgtggatat
      781 gaccgctggg ggacgtatag actcaactcc ttacaaagaa aatgtagggc tgcttgctgt
      841 tctccatgca accaataacg cacgagcaat attaggtaaa acgggtaaat taactggagt
      901 tcttcctgcc tatgggaatg gaggagggat cgttgcaact aatggtggga ccggtgttaa
      961 tgaaggggtc attgatgcca ttggtactga aatgattgcc tatcaagaca gtaccattgt
     1021 taacgatggt acactttttg tttgggataa taatgataaa tatgctctcc aggcagaggg
     1081 gatggttgcc ggtagtaatg gttcttcagc cattaataat ggtgttatta atattcgccc
     1141 atttaaaaat gctttcgctc cagaggggat taacaccgca attgttgtta gtaatggggg
     1201 catggcaaca aataaaggca caataaatat tactgccgat gcatcaacta atgataataa
     1261 tggcaaaaca cgaggtgtaa atgttggagc tggaggctct tttattaact cggccttcgg
     1321 gagcatcaat gtaggtattg cggaggataa aactgcgact cattcggctg tgggttctgt
     1381 tgcgattgaa gtacaaaatg gtgcaaacaa agtcgttaat gaaggtacta tttttttggg
     1441 caggggggct caggggaact acggaatcct ggcaaaggat gccgggactg ttgatgtggt
     1501 aaataaaggg actatcacta ttgacggtca tgacagtgat gcacctgcac tgaatgttgg
     1561 catgctggca aataatagct ccgggatgaa aaactccggg attatcaatg ttaatggtct
     1621 gaacagcacc gggctacagg taatcaatgc aggacagttg aattctgacg gtacgataaa
     1681 tgttggcggc aagggtatca gtagtggttt ccgtaactat ggtgcctggg tggaaggtgc
     1741 cggaagcaat gttaatgtat ccggaaaaat cagnnnnnnn nnntgtgggg gtttttgcta
     1801 aagatggcgg cagtctgacc ctgtcaggca atggtgcagt gctatttggc agcagcgatc
     1861 agataggctt ttatgtctat ggaaaggact ctgccattca taataccgga agcggtgtta
     1921 tggatgtgtc cactgaaaac tcaacattat tccgtattgc cagtggtgcg acattccagg
     1981 gaactgcaga tgcttcttct gcacttacgg cgtctggtaa gaactcttat gcacttattg
     2041 ccacggggaa atcggatggc ggtgtggcct cgacagtaac gtctggagga atgaccatca
     2101 acctgacggg tgagggggct acagcgactt taattgaagg gggagcgcag ggcacaattg
     2161 aaagtaatgc cattatcaat atggataatg ccagtgcgat agccggtatt gcggatggca
     2221 atggctatga tatttccggc aaactcatta atccgaagga caagaccaca ctattaacgg
     2281 cgggggctca gttaagttcc acccaggata aagtgaccgg gtatatcgcc cgtaatgggg
     2341 ccactctaaa taataccggt aatatcatct ttactggaaa gaatacagtg ggcgtccggg
     2401 ttgaggaggg ggctgttggt accaacagcg gaaatattac agttcaggat ggtggtgtgg
     2461 gactaattgc taatgccaca caagatgtta caacgattaa taactcggga aatctcgttc
     2521 tcaagggagg agataatgct aaccgtacaa cgggtataaa agcatctggt acaacaacaa
     2581 cggttaatat gaccgcgggt actatatctt tgcagggaca gggggcgatt ggcgttgagg
     2641 ccagcaataa agggactgtt aaccttgatg gttcggcagt accgaacttt gctgctgacg
     2701 gctctggtat taccgatcag attgcttttc gtattatcgg agatggtgca accattaaga
     2761 cgaatattgc accgggaact ctgctggatg ccagtgggga acgttctgta cttttccgta
     2821 ttgaagatgg ggcaaaacag gccggctctc tgctgatgaa aacctccggg acaggcagcc
     2881 gtggtatctg ggccacaggg aaagggagca atgtcctggc tgatgctggc agtgatttcc
     2941 agatcctggg cgctcaggct cagggattat atgtaactgg tggtgcgaca gcgacgctga
     3001 aacagggggc atcagttaac cttgtagggg atggcgctgt tgtcgcggaa gttgacggaa
     3061 atgaatacgc tctggatggc agtattacac aaacgaatac tggctcggtt attaccaatg
     3121 aggcagatat ctcttcgccg ctgaataatg ccaagggctt tattacgcgt aatcagggac
     3181 tgttgattaa caacggcaac attgatttca ctaccggtac agataatatc ggcgtctggg
     3241 ttgataacgg ccgctttgaa aatacaggaa nnnnnnnnnn ggtcaatggc gttgcattat
     3301 ttgttgaagg tgcacagtct cagattacca gcacaggagg ggatatcgtc gctgtggatg
     3361 gtgaggctgc cattaagctg ggggcgggcg cgtcactgaa cctggcaggg agtggcttgg
     3421 gtacgatcga aggtcagaaa aatgcgcatg gcatcctgct ggatacaggg gctgtggggc
     3481 tggttattga tggtgcgaaa atcaatgtta atgctgcagg tgcggtcggt cacgggattg
     3541 agaaccgcgc agaaattgaa ggcattcagt taaccaatac gactgaaatt aatgtggctg
     3601 atggcattgg tgtacgtact tctgcctccc tggccaagac caacagcggc actattaatg
     3661 tagacggcag tggaattgca ctggcgttcc agaaagctga cggaagtgaa accgataata
     3721 acctggatat gtctgactcc gctggattgg tcattaacct gaagggtacg gacggcacgg
     3781 gtattttcgc caacactaaa gatggtgctg tcgtgaagag tggtgcaagt gtcaatgtta
     3841 tacaggccga tggcggttcc gctctggtgg ttaacaatgc agccagtgaa gtggttcaga
     3901 gcggtaatct catctctgct tctctgagtc atgccgtagt ggatgcttca aaagcacaat
     3961 cctttaccaa taaaggtcag attaaagctg cgtccaccac cgggactgca atggcgtttg
     4021 atgacgccgt gaataccacc gtactgaatg acagcggtgc tgaaattcag ggggttgtgg
     4081 ctctgaacgg cggtgataac acattcacca ataaaggcag tattaccgga accgtcagtg
     4141 cgaaagaggg taacaataca tttttatttg atgatggcag cacactgaca ggagaagtga
     4201 ctgcaggaaa tggcaataat aatgtaacac tcaatggtaa gactcatgtt gatcaggtta
     4261 ctgccggtac cgggaagaac accttcacca ttaaaggtga aggggcaacc tggaacctgc
     4321 tggatggcgg gcagggagat tctgattccc tgatttttga taacgccatt catacgctgg
     4381 attctgttgt aaaactacag aatttcgaac atgtcgggct gaagaacagt tcacttgtca
     4441 ctctgaagga agctcttgtg ctgaccgatg gggggaacgg tccgggttcc gtcgatattg
     4501 aatcgggcag cgaactggcc attattcccg cagttgcagg caactttacc tttgatccac
     4561 tgttaacagg caaaggaaca ctgtctgccc gtcttgatgc cgacacatct gcttttgaat
     4621 tcagccataa cgtcggggat caatttgccg gaactctgaa gctgggtact agtagctttg
     4681 ctctggaagg gctgaatacg agcgggttaa cccatgcaat gctgatgtct gaaaccggga
     4741 atatcacaac ggttggctcc ggtgttcagc agattggcgg tcttgggttc aatggcggaa
     4801 cgctgatttt tggttccgtt atgccgggcg ataccatagc cagcaacagt attgaaacct
     4861 ctgctgcagg tacgctggat atccggggga aaggcacaat tcaggtcacc atgccagatg
     4921 aagtgattaa tgatattccg gctgttgata cccgtaagaa tttgctggag caggatgatg
     4981 cgcagaccct ggtcacgctg gtgaatgcag cgggtaccgt caccggtact ggcgggcaac
     5041 tgcaactggt ggatgaaaac gggcaggcta tttctcacag tcagacgttt gatgtcactc
     5101 agggcggtga agttgtagct cagggaaatt atgactataa gctgctggga agctccaacg
     5161 gtattaaagg tgacggactg tacgtaggct atgggctgaa gtcgctggat ttacagggaa
     5221 ccggtgatca agcgctggtg ctgacaccga gagcgaatgc ccagggactg cagacagatc
     5281 ttggcgcaca gttaacgggg gcaggggatc tggccatcga agctgcgggg caggttgtca
     5341 cactgtctaa cggcggtaat aactacaccg gggatacgct ggtgcgcagc ggcacattac
     5401 agatggcaaa tgataatgta cttggcgcaa caggtaatct gaacgtcgcc agcaatgccg
     5461 tcttcagaag aaacggttac aggcagactg tcggtgcact gcagacagaa acgggagcgc
     5521 acattcagct tgatagcggt agtgtactaa cggtcagcgg cacgcagcgt cagccggggg
     5581 atgataatgg tggcatcatt gaaaacaacg tgctgtccgg tgagggaacg ctggctgtca
     5641 cgggcagcaa tctgacggta catggcacca atatcgggtt taccggtaat gccagcctta
     5701 cccagggggc gctggttgaa atgaacggcg cacaggggct gggaagccag ggctccatca
     5761 gctttgaaag ccttaatgat cgcctggcga ttgatatcgc cgacggtagc ggagtcagca
     5821 gtaacctgag taaatcgctt tccggagaag gttcagtggg tatactgaac accactgatc
     5881 tgacgctttc cggggataac agtaatttca gcggtgaatt ccgtgtgcag aaagatgctg
     5941 ctctgcgggc ttcagatgaa aaacacctgg gcaccgggct gattgacagt gatggtgtca
     6001 cctggcttac cgccagtgga aactggttac tgaagaatga tattactggc tccggcgctc
     6061 tggtgaaaca gggggcgggt aatctgatca ttaatcatga gctgacttat accggtgata
     6121 ccacggttga gagtggcgtt ctgattgtcg gtgatgattc agtcacccgt gccgctggcg
     6181 cgacgttgtc aggatccaaa aacattcatg ttcttaatgg cggcacactg agtgggctgg
     6241 ggaccgtttc gggtcaggtt aataaccagg gaacactggc atcactgaat gctctctcag
     6301 gttatgaaac ggcagaggtg ggcaatttta ccgttggctc actgaccaat accggcgtca
     6361 ttcgtctggc cggtggtaag acaggaaata ccctgaccgt aaatggcgat tacaccggtg
     6421 gcggtacgtt aattatcaat accgtgctgg gggatgatac ttctaccact gacaaactga
     6481 ttgtcacggg taacacctcc ggtgataccg gcgtggtggt gaataatgtc cggggccagg
     6541 gggctcagac cgctgacggc attgagattg tgcatgtcgg tgggcagtct gacggtaact
     6601 tcagactgca gaaccgtgcg gtggcagggg cgtnnnnnnn nnngtaatgc gggtggtact
     6661 gacggtaact ggtatctgcg ttctgaattg ccgccagaac cacaaccgca gccgcagcca
     6721 caaccgcagc cacagccaca ccccacgccg gataaacctg tacagaaggt ctatcgtccg
     6781 gaagcgggct cttatattgc gaacatagcg gcagccaaca cgctgttcaa catcagaatg
     6841 cacgatcgtg agggggaaac ttactatacc gatgtgttta ctggtgagaa gaaagcaaca
     6901 agtatgtgga tgcgtcatat cggcggtcat aatcgctgga aggacagcag ttcacagctg
     6961 aatacgcaaa gtaaccgtta tgttgtgcaa ctgggtggca gcattgcgca atggactgac
     7021 ggtcaggacc gtctgcaact ggggattatg gccggttatg gaaatgagaa gagttccacg
     7081 acatcctcgc tttcgggata taaatctaaa ggtgcgatca atggatacag caccggttta
     7141 tacggaacgt ggcagcagaa tgacggtaat gacaacgggg cttatgtcga tacctggatc
     7201 cagtacggat ggtttaataa taccgttaat ggtgaaaaac tggctgcgga gtcctggaaa
     7261 tcccgtggat ttaccggttc agtggaagcg ggttatacct ttaaagccgg cgaatttacc
     7321 ggtagccagg gtagtcacta tgactggtat attcagccgc agtcccagat cacgtggann
     7381 nnnnnnnncg tggatgaatg taagagccag tgagcatacg gagaagaatg gcacgaaagt
     7441 tcagctcagc ggagacggta atatccagag tcgtctgggc gtcagaactt acctgaaagg
     7501 taagagtgcg tctgatgata ataaggcgca tcagtttgag ccatttgtgg aagttaactg
     7561 gatccataat acacggagct ggggtgtcaa aatggataat acggcactca gtcaggacgg
     7621 agctacaaat attgctgaag tcaaaacggg tgttcagggt aaattgtcag ataacctgaa
     7681 tgtctggggc aatgttggtg ttcaggctgg cgataaaggg tacagtgatg cccaggcaat
     7741 gctgggtatt aaatatatat tctaatcaca cagtggcagg aggggacctc ctgccacctc
     7801 agggttgtca taatgagttc tgtaatgaaa ttttctgttg ttaatgcagt agtgctgctg
     7861 ttgctgattg tacttactgg tgttttttat atgaaaaagt caaaagaaat tgcagttgtt
     7921 aatatcgatg ctatttataa tcagtctgcc gccggagaca tggtcagaaa gcatctgaat
     7981 gaggttaaga gtattcttga aaaaggctat gaggatgcgc aaaaagcatt cgcagataac
     8041 gcgaataaag aggcgctgct tcttgatgcc agacaaaaac tggagcgaca atatgttatt
     8101 gaggaaaata gcgcaaaaat ggtgttatcc cgtgcgatag ataatgccat aggtacatgg
     8161 agaaaagaca atcctaaaaa tattgctgta ttgtcttcag gtgttgtgct tgcgcataac
     8221 gagtcagctg atatcaccaa agaagtcatg gctctggttg aaaaagaaaa tctggttttt
     8281 aatcagttac ccaaagtgga agtgcacaat aagaatgaag agaaaccaga acaaagtaag
     8341 taatcactag attctgacga tttccagaag ggagattcag caggccagca tctgtaatca
     8401 ttcagatttg ctggcttgct gctaaatatg gttgctaaga aaaatagcag gccagtaatt
     8461 tattcagatt actgaatata ataatttttc aggaacggtt atgctgaagg tagtatgttt
     8521 aatcgacaca attatttgct gtgtctaata tgaggtgaca tttttatggt cactgaccag
     8581 caagactgac ccgtattgat tagcctgatt attggcaact tattggggta gtcattttga
     8641 catcgaatgg gggaggtttc cagttaagtt cacgaatgca tgatgatttg cgttcacata
     8701 tcgaagtact ggttgactat actaaggatt ttaagataac tcactgattg taagtgggtt
     8761 attgcgaggg ggatgataga tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
     8821 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
     8881 tggactacgg ggtaagtaca cagctgctcg attgcttaaa ccctcgccat ttatgccggg
     8941 tttttatcat ttttcttaat gttttccgca cgttctgctt tttgacgtgc ttctgctttg
     9001 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcaaa gataaaggtg
     9061 ccgccgcaga tgttccccgc taaagtaggt agtgcaaagg gccagatgaa atcgctccag
     9121 tgcagcgtgc cgttaaacac cagatagagg atttcaacag aaccgacaac gatgtgggtg
     9181 gtgtcaccca gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcaccc
     9241 gctgcaggaa acatccatac catagtggcg atcagccagc cggaaatgat cgcgttggca
     9301 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     9361 gtttcttcat tgaagatagg catatattca aatgcccacg cagcaatacc tgtcccgaga
     9421 atattaccca gcagcacgat gccccataac cgcataagta agccgacgtt gctcattgtc
     9481 ggtttttgca tgacgggtag taccgcagtt acggtgtttt cggtaaataa ttgctggcgg
     9541 gccattatga cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc
     9601 ggcacgcctt ccagttcgac atgaaatatc ccttttgcca gtagcgaagc gcccatcgac
     9661 aggcccgccg caatggctga ccacagtagc gccattgcgt cgcgttccag ctctttttca
     9721 ccatcctggc ggatatgctc atgaattgcn nnnnnnnnnt tgccatcgcc cgggagggga
     9781 gtcggtcttc atctatttct atttttttac cgcgctcttt ttcttcgctc tcaacttcaa
     9841 tttcgtcgct gtgttgatca attttgtcgt tgtccatggt ctcttctctt gaattagcac
     9901 gtatagctaa agcgtagcgg cttttttgct cgcaactggc gggagttact ctgaaaatgt
     9961 agaaaaggcc gcgtttgcct ttttctgttt ctattgaatc aagtagccta cagggcggcg
    10021 attaccaggc tatgatcaaa tcagcaaatc agggcgtctg gacatcagtt gacgtgctgt
    10081 tacaatcgcc cacacctaaa caggcggata cggtatcgtt ccgtcatgga tggcaaactg
    10141 cataagccat aaaaaaacag ggagacattt atgaagcttc gcctgtcggc gcttgctctg
    10201 ggaactacgc ttctggtggg gtgtgcgagt tccggtacag atcagcaagg gcgttctgac
    10261 ccgttagaag ggtttaaccg cactatgtac aacttcaact tcaatgtatt agacccgtat
    10321 attgttcgac cggtcgctgt cgcctggcgt gattatgttc cgcaacctgc gcgtaacggt
    10381 ttgagcaact ttaccggcaa ccttgaagaa cctgcggtga tggttaacta cttcttgcag
    10441 ggcgaccctt atcaggggat ggtccacttt acccgctttt tcctgaatac cattttggga
    10501 atgggcggct ttattgatgt tgcagggatg gcaaacccga aactgcaacg gactgaacct
    10561 caccgcttcg gtagtacgct tggtcattat ggtgtgggtt atgggccgta cgttcagtta
    10621 ccgttctacg gtagcttcac gctgcgtgat gacggtggtg atatggcgga tagtctttac
    10681 ccggttcttt cctggctgac ctggccgatg tctgtgggta aatggacgct tgaagggata
    10741 gaaactcgtg cgcagttgtt ggattccgac ggtctgctgc gtcagtcgtc cgatccttat
    10801 attatggtgc gtgaagcgta cttccagcgt catgatttca tcgctaatgg cggtgaactc
    10861 aaaccgcagg aaaatccgaa cgcgcaagcg attcaggatg atttaaaaga tatcgattct
    10921 gaataagaaa caaacaaaaa aggtgagtcg caaaactcac cttttttctt attaagcgaa
    10981 cgcgttatca gaacgcgtag ttaaagttnn nnnnnnnnaa agttagtacc gaacagccag
    11041 gctttacctt cagactcgaa ctggtatggg ccttcgttaa ttttcacgct ctgaccgtgc
    11101 atataagaaa caccaacatc gactgaagca tctttattaa atgcgtaagt cgtacctgca
    11161 ctcagccaga aacggtcctg gtccggaatg gagatagaac gattctgtgc cggaactggg
    11221 ctgtcatcaa aggcgatacc ggtacggaag gtccagttat catcgtagta ataagtggtt
    11281 cccaacgcga tgcggtaagc atctttaaag ccttcatgtt tctggaacag cgtgtcgcca
    11341 ctcgttgagg tcgctttcag ctgctggaac tgactccagc tggtgtaagc caggctatag
    11401 tgaatcgccc actgcggatc aacacggtta taacccgaca cttcccacat ttcaggcagg
    11461 ttcagcgtca gataacccga ttgcgttgcg ccacctgtcg cggtaggaat tggcaaaccg
    11521 tagttattaa aagccgggtt aagatcgnnn nnnnnnnttg aagtcaattt tcacttcaga
    11581 gcggtaggtc agtgcatagc ggttattttt atccagttca tacaggattc cggcgttcca
    11641 gccaaaaccc cactggttac cgttcagatg agcgattttg gtattactgt cgataccgtt
    11701 ggcggtagct gccaatgctt gcccttgctg agtttgtcca gcaggagatt gcataatctg
    11761 accggcaacc agctgcccca gatcgcctgc gaaacgttca attttcgcgc gagcgtagac
    11821 ggcgttgaaa ccaagaccaa agctccatgc attatttaag cgatacgcac cgcttaagtt
    11881 caggttcatg gtttcaaggt cggttgtacc accgacagaa ccgcctgcat aagtatcgtt
    11941 aaactctgtt gccagaccat a
//
LOCUS       DAWDRC010000040        11888 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2787_length_11888_cov_2.531818, whole genome shotgun sequence.
ACCESSION   DAWDRC010000040 DAWDRC010000000
VERSION     DAWDRC010000040.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2787_length_11888_cov_2.531818"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8098..8107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9768..9777
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2829 a         2823 c         3305 g         2911 t
ORIGIN      
        1 ctgcgacttc tacgccagca ccaaaagaga cggcgactac ggctccagta cagacggcat
       61 ccccggcgca aaccacggca acaccagccg ctggagggaa gaccgcaggt aatgttggtt
      121 cgttgaaatc ggcaccgtcc agccattaca ctctgcagct gagcagttcc tctaactacg
      181 acaacctgaa cggttgggcg aagaaagaga atctgaaaaa ctacgttgtc tatgaaacga
      241 cgcgtaatgg tcagccgtgg tatgtcttgg tttctggcgt gtatgcttcg aaagaagagg
      301 cgaaaaaagc ggtatctaca ttgccagcag atgtccaggc caaaaacccg tgggcgaaac
      361 cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt atctcccgca atggtttatc
      421 attgcgggcg ttgcctgaag cgctggatgc tgtcggagct ttctccacag ccggagaagg
      481 tgtaattagt tagtcagcat gaagaaaaat cgcgcttttt tgaagtgggc agggggcaag
      541 tatcccctgc ttgatgatat taaacggcat ttgcccaagg gcgaatgtct ggttgagcct
      601 tttgtaggtg ccgggtcggt gtttctcaac accgactttt ctcgttatat ccttgccgat
      661 atcaatagcg acctgatcag tctctataac attgtgaaga tgcgtactga tgagtacgta
      721 caggccgcac gcgagctgtt tgttcccgaa acaaattgcg ccgaggttta ctatcagttc
      781 cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg cggtactgtt tttatatttg
      841 aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc gcggtgagtt taacgtgccg
      901 tttggccgct acaaaaaacc ctatttcccg gaagcagagt tgtatcactt cgctgaaaaa
      961 gcgcagaatg cctttttcta ttgtgagtct tacgccgata gcatggcgcg cgcagatgat
     1021 gcctctgtcg tctattgcga tccgccttat gcgccgctgt ctgcgactgc caactttacg
     1081 gcgtatcaca caaacagttt tacgcttgaa caacaagcgc atctggcgga gatcgctgaa
     1141 ggtctggttg atcgccatat tccagtgctg atctccaatc acgatacgat gttaacgcgt
     1201 gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc gacgcagtat aagcagcaac
     1261 ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt acaaaccagg agtcgtttca
     1321 cccgcgaaaa aataattctc aaggagaagc ggatgaaaca gtatttgatt gccccctcaa
     1381 ttctgtcggc tgattttgcc cgcctgggtg aagataccgc aaaagccctg gcagctggcg
     1441 ctgatgtcgt gcattttgac gtcatggata accactatgt tcccaatctg acgattgggc
     1501 caatggtgct gaaatccttg cgtaactacg gcattaccgc ccctatcgac gtacatctga
     1561 tggtgaaacc cgtcgatcgc attgtgcctg atttcgctgc cgctggtgcc agcatcatta
     1621 cctttcatcc agaagcctcc gagcatgttg accgcacgct gcaactgatt aaagaaaatg
     1681 gctgtaaagc gggtttggta tttaacccgg cgacacctct gagttatctg gattacgtga
     1741 tggataagct ggatgtgatc ctgctgatgt ccgtcaatcc tggtttcggc ggtcagtctt
     1801 tcattcctca aacactggat aaactgcgcg aagtacgtcg tcgtatcgac gagtctggct
     1861 ttgacattcg actggaagtg gacggtggcg tgaaggtgaa caacattggc gaaatcgctg
     1921 cggcgggcgc ggatatgttt gttgctggtt cggcaatctt cgaccagcca gactacaaaa
     1981 aagtcattga tgaaatgcgc agtgaactgg caaaggtaag tcatgaataa gtttgaagat
     2041 attcgcggcg tcgcttttga tctcgacggt acgctggttg acagtgcacc cggtcttgct
     2101 gctgcggtag atatggcgct gtatgcgctg gagttgcctg tcgcaggtga agaacgcgtt
     2161 attacctgga ttggtaacgg cgcagatatt ctgatggagc gcgcattgac ctgggcgcgt
     2221 caggaacgtg cgactctgcg taaaacaatg ggtaaaccgc ccgttgatga cgacattccg
     2281 gcagaagaac aggtacgtat cctgcgtaaa ctgttcgatc gctactatgg cgaggttgcc
     2341 gaagagggga cgtttttgtt cccgcacgtt gccgatacgc tgggcgcatt gcaggctaaa
     2401 ggcctgccgc taggcctggt caccaacaaa ccgacgccgt tcgtcgcgcc gctgctcgaa
     2461 gccttagata tcgccaaata cttcagcgtg gttatcggcg gtgatgatgt gcaaaacaaa
     2521 aaaccgcatc cggacccgct gttactggtg gctgagcgga tgggaattgc cccacaacag
     2581 atgctgtttg ttggcgactc acgcaatgat attcaggcag caaaagcggc aggttgccca
     2641 tcagttggct taacctacgg atataactac ggcgaggcta tcgatctcag ccagcctgat
     2701 gtaatttatc agtctataaa tgaccttctg cccgcattag ggcttccgca tagcgaaaat
     2761 caggaatcga aaaatgacta agcccatcgt ttttagtggc gcacagccct caggtgaatt
     2821 gaccattggt aactacatgg gtgcgctgcg tcagtgggta aacatgcagg atgactacca
     2881 ttgcatttac tgtatcgttg accaacacgc gatcaccgtg cgccaggatg cacagaagct
     2941 gcgtaaagcg acgctggata cgctggcctt gtatctggct tgtggtatcg atcctgagaa
     3001 aagcaccatt tttgttcagt cccacgtacc agaacatgcg cagttaggct gggcactgaa
     3061 ctgctatacc tacttcggcg aactgagccg catgacgcag tttaaagata aatctgcgcg
     3121 ttatgccgag aacatcaacg ctggtctgtt tgactatccg gtgctgatgg ctgcggacat
     3181 cctgctgtat caaactaatc tggtgccggt gggtgaagac cagaaacagc acctggaact
     3241 gagccgcgat atcgcccagc gtttcaacgc gctgtatggc gagatcttta aagtgccgga
     3301 gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg ctggagccga ccaaaaagat
     3361 gtccaagtct gacgataacc gcaataacgt tatcggcctg ctggaagatc cgaaatcggt
     3421 agtgaagaaa atcaaacgcg cggtcactga ctccgacgag ccgccggtag ttcgctacga
     3481 tgtgcagaac aaagcgggcg tttccaacct gctggatatc ctttctgctg taacgggcca
     3541 gagcattccg gaactggaaa aacagttcga aggcaagatg tatggtcatc tgaaaggtga
     3601 agtggctgat gccgtttccg gtatgctgac tgaattgcag gaacgctatc accgtttccg
     3661 caacgatgaa gccttcctgc aacaggtgat gaaagatggc gcggaaaaag ccagtgcaca
     3721 cgcttcccgt acgctgaaag cggtgtacga agcgattgga tttgtggcga agccgtaatt
     3781 taacattaat gccaaaaacc ggggaaaccc ggttttttta tcctcttttt gcaataatcc
     3841 gaaaaaatgt gaagcgcctc gccgtttcca catctcgctg ttgcgcctta tcttcaaatt
     3901 cctgaagata taaacattca ttttattgaa aatatattat gcgtcgaacg tttatcaaaa
     3961 aagaaggcgt cgttatcacg acactggccc gttatttgtt gggtgaaaag tgcggtaatc
     4021 gactgaaaac catagatgag ctggcaactg aatgccgttc atcggttggc ctgacgcagg
     4081 ccgcgttgaa aatgctggaa tcaagcggag cgatacggat tgaacgccgt gggcgcaatg
     4141 gcagttatct ggttgagatg gataacaaag cattgctgac tcatgtggat atcaacaacg
     4201 tggtatgtgc gatgcccttg ccctataccc gtttgtacga aggcctggcg agcggattga
     4261 aagcccagtt tgatggcatt cctttttact atgcgcatat gcgcggcgcg gatattcgcg
     4321 tggagtgttt acttaatggc gtgtatgaca tggcggtggt ttcgcgactg gcggcggaaa
     4381 gttatctcac gcaaaaagga ttatgcctcg cgctggagtt gggaccgcat acctacgttg
     4441 gcgagcacca gttgatttgc cgtaaaggcg agtccgcaaa cgtgaagcgc gtggggctgg
     4501 ataaccgttc ggcagatcag aaaatcatga ccgatgtctt ttttggcgat agtgatgtgg
     4561 aacgagtcga tctctcttat cacgagagtt tacaacgcat tgttaaaggc gatgttgacg
     4621 cggttatctg gaacgtagtg gcggaaaacg aactgactat gctgggatta gaagcgacgc
     4681 cgctcacaga cgatccgcga tttttacagg ccaccgaagc cgtgatcctg acgcgtgccg
     4741 atgattaccc aatgcaacaa ctgctgcgtg cagttgttga taaacatgcc ctgcttgctc
     4801 atcaacagcg agtagtcagt ggggaacagg aacccagtta ttaaacgaaa ggcatctgat
     4861 atggaaacca gactcaacct gctttgcgat gtaggcgtta ttgataagga catctgcaaa
     4921 ggcatgatgc aggtcgtcaa tgtactggaa acagagtgcc atctaccggt gcgcagtgag
     4981 caaggaacga tggcgatgac acatatggcg agtgcactga tgcgcagtcg ccgtggtgaa
     5041 gaaatagagc cgctggatga cgaattgctg gcggaactgg cgcaatccag ccactggcaa
     5101 gccgttgtgc aattgcatca ggtattgttg aaggaatttg cactggaagt taacccgtgt
     5161 gaagaaggct atttgctggc caacctttat ggattatgga tggctgctaa cgaagaggtt
     5221 tgaagctccc gataaatcca cgtcgtacaa cctaaatatt caaaaaaatg aatatttaat
     5281 tcaaggaaca gaaacgaggc aacaagatgt ttgtagaagc attgaaacgc cagaatccgg
     5341 cgctgatttc cgccgcacta agtttgtggc agcagggcaa gatcgccccg gacagttggg
     5401 tgatcgacgt ggatcaagtt ctggaaaacg gcaagcggct gattgagacg gcgcggcttt
     5461 acggcattga actgtatctg atgaccaaac aattcggtcg taatccgtgg ctggcagaaa
     5521 aactgctggc tctgggctac agcggcattg tggcggtgga ttacaaagag gcgcgagtca
     5581 tgcgccgcgc cggtttgccg gtagcgcatc aggggcatct ggtacaaatc ccttgtcatc
     5641 aggtttctga cgccgttgaa cagggaaccg acgtcatcac tgtgtttacc ctcgacaaag
     5701 cgcgggaaat ttctgcggca gcggtgaaga ccggacgtgt gcagtctgtg ctgctcaaag
     5761 tttatagcga cgatgatttt ctttatccgg ggcaggagag cggttttgta cagcattcgc
     5821 tgcacgaggt ggttgctgaa atccagaatc tgccggggct acatttggcg ggacttaccc
     5881 atttcccttg cctgctttgg gatgaggctg ccgggaaagt tttgccgaca ccgaatcttc
     5941 acacgctggt acaagcacgg gatcaactgg cgaaatctgg tattgcgatt gagcaactga
     6001 acgcgccttc agcgaccagc tgcacgtcgc tgccattgct ggcggaatac ggtgtgactc
     6061 ataccgaacc cggtcatgcg ctgacgggta ctattccggc aaatcaacag ggcgatcagc
     6121 ctgaacgtat cgcgatgctc tggttaagtg aaatctccca tcatttccgt ggcgacagct
     6181 actgctacgg cggcggttac taccgtcgtg gtcacgcgca acatgcgctg gtgtttacgc
     6241 cagaaaatca aagaattact gaaacatatc tcaacgccgt tgatgacagc agtatcgact
     6301 acaccctgcc gctggcaggc gagcatccgg taagcagtgc agtggtgctc tgttttcgca
     6361 cgcagatttt tatcacccgt agcgatgtgg tgctggtgtc cggtattcac cgtggcgaac
     6421 cagaaatagt tggtcgttat gacagcctcg gcaaccctct ggaggcgtaa atggcgcgat
     6481 ttgtggtgtt agtgattgat agctttggcg taggggcaat gaaagatgtc acgttggtgc
     6541 gtccgcagga tgccggggcg aatacctgtg gtcacatcct gagccagttg ccgcatttgc
     6601 agctaccaac gctggagacg ctggggctaa tcaacgcatt gggttatgcg cctggcgata
     6661 tgcagccgtc agactcctcc acctggggcg tggcggaact gcaacatgaa ggtggcgata
     6721 cctttatggg gcatcaggag attttaggta cgcgcccatt accgccgctg cggatgccct
     6781 ttcgtgatgt gattgaccgt gttgagcagg cattagtatc cgccggttgg caggtggagc
     6841 gtcgtggcga tgatctgcaa tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc
     6901 tcgaggcgga tttaggccag gtctataaca tcaccgccaa tctctctgtg atctcttttg
     6961 acgacgcaat caaaatgggt cgtattgtgc gtgagcaggt acaggtcggt cgggtcatta
     7021 catttggtgg cctgttaacc gacagccagt gcatcctcga tgccgcagaa agcaaagaag
     7081 ggcgctttat tggcatcaat gcgccgcgtt ctggcgccta tgacaacggt ttccaggtcg
     7141 tacatatggg ctacggtgtc gatgaaaaag tgcaggtacc gcaaaaactg tatgaagcgg
     7201 gcgtgccaac cgtgctggtg ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga
     7261 gctggcaaaa cctggtggat agccagcgga ttatggatat caccctcaac gaatttaaca
     7321 cctatccgac ggcgtttatt tgtaccaata tccaggaaac cgacctcgct ggtcatgcag
     7381 aagacgtcgc acgttatgcc gaacgtttgc aggtcgttga ccgtaacctt gccaggcttg
     7441 ttgaggcgat gcagccagat gattgcctgg tcgtgatggc ggatcacggt aacgatccga
     7501 ccattggtca cagccaccat acccgcgaag tggtgccagt gctggtttat cagcaaggga
     7561 tggtcgctac gcagcttggt gtgcgcacca cgctttctga tgtgggggct accgtatgtg
     7621 aatttttccg cgcgccaccg ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg
     7681 caggagacac cctatgagtt ttgatccgac gggttacacc ctggcccatg agcatctgca
     7741 tattgatctc tccggcttta aaaacaacgt ggactgccgc cttgatcagt atgcgttcat
     7801 ttgccaggag atgaacgacc tgatggcccg gggcgtgcgt aatgtgattg agatgaccaa
     7861 ccgttacatg gggcgcaatg cgcaatttat gctcgatgta atgcgcgaaa ccgggatcaa
     7921 cgtggtggcc tgtaccggtt attaccagga cgcgtttttc ccggaacatg tggcgacccg
     7981 cagcgtgcag gaactggcgc aggagatggt cgatgaaatt gagcagggta tcgatggcac
     8041 tgacctgaaa gccgggatca tcgcggagat tggctccagc gaagggaaga ttacgccnnn
     8101 nnnnnnnaag agaaggtgtt tattgccgcc gcgctggcac ataaccagac cggacgcccg
     8161 atctccacgc atacgtcgtt cagcacgatg gggctagagc aactggctct gttgcaagcc
     8221 cacggagttg atctttcgcg cgtcaccgtc ggccactgcg atctgaaaga caatctcgac
     8281 aacattttga agatgatcga tctcggcgcg tatgtgcagt tcgacaccat cggcaagaac
     8341 agttactacc cggacgaaaa gcgcattgcg atgctccatg cgctacgtga ccgtggggtg
     8401 ctgaatcgcg tcatgctgtc gatggatatt acgcggcgct ctcatttaaa agccaacggt
     8461 ggttatggct atgacttttt attgaccact tttattccgc aattgcgcca gtcaggattc
     8521 agtcaggccg atgtggatgt gatgttacgt gaaaatccct ctcaattttt ccaataagga
     8581 cagactcatg aaaaagattg gcgttgcagg cttacagcgt gagcagatta aaaaaaccat
     8641 tgaagcgacg gctcctggct gttttgaagt tttcattcac aacgacatgg aagcggcaat
     8701 gaaagtgaaa tccgggcaac tggattacta catcggcgcg tgtaataccg gtgcgggcgc
     8761 ggccttgtcg attgctatcg cggtgattgg ctataacaaa agttgcacca ttgccaagcc
     8821 gggcattaaa gcgaaagacg aacatatcgc caaaatgatc gctgaaggaa aagtggcgtt
     8881 tggcctttcc gttgagcacg ttgaacacgc gattccgatg ctgattaacc atctgaaata
     8941 aaaggcacga ctatggatct gtatattcag attatcgtgg tggcgtgcct gacgggtatg
     9001 acatcgcttc tggcgcatcg ctcggcagcc gtttttcatg acggtatccg cccgatcctg
     9061 ccgcaactga ttgaaggcta tatgaaccgt cgcgaggcgg ggagtatcgc ttttggtctg
     9121 agcattggtt ttgtggcctc ggtggggatc tcttttaccc tgaaaaccgg gctgctcaac
     9181 gcatggttac tctttcttcc taccgatatc ctcggcgtac tggcgataaa cagcctgatg
     9241 gcgtttggtc ttggtgctat ctggggaata ttgatcctca cctgtctgtt gccagtaaac
     9301 cagctgctga ccgcgctacc ggtggatgta ttaggtagcc tgggggaatt aagctcgccg
     9361 gtggtttcag cttttgcact gttcccgttg gtggcgattt tctaccagtt tggctggaag
     9421 caaagtctga tcgccgccgt ggtggtactg atgactcgtg tggtggtagt gcgctatttc
     9481 ccacatctta accctgaatc catcgaaatc tttattggca tggtgatgct gctggggatc
     9541 gcaataactc atgacctgcg ccatcgtgat gaaaatgata tcgatgccag cggtatgtcg
     9601 gtgtttgaag agcgtacatc acggattatc aaaaacttac cgtatatcgc catcgtgggt
     9661 gcattgattg ctgccgttgc cagcatgaag attttcgccg gtagtgaagt gtcgatcttc
     9721 acactggaga aagcgtactc cgcaggcgta acgccggaac aatcgcannn nnnnnnncca
     9781 ccgcgttagc caccggtgtg tatgcagttg cgggctttac ctttgtttat gcggtgggct
     9841 atctctcgcc gaatccgatg gttgcagcgg tattaggcgc agtggttatt tcggcggaag
     9901 tcttgctgct tcgttcgatc ggcaaatggt tggggcgcta cccgtcggta cgtaatgcgt
     9961 cggataacat ccgtaacgcc atgaatatgc tgatggaagt ggcgttgctg gtcggttcga
    10021 tctttgcggc aatcaaaatg gcgggctata ccggattctc tatcgcggtt gccatttact
    10081 tcctcaacga atccctgggc cgtccggtac agaaaatggc ggcaccggtc gtagcagtaa
    10141 tgatcaccgg catcctgctg aatgttcttt actggcttgg cctgttcgtt ccggcttaag
    10201 gaaggcacct atgaagacgt ttcctctgca aagcctgacg cttattgagg cgcagcaaaa
    10261 gcagtttgcg ctggtggaca cgatttgccg tcattttcct ggcagcgagt ttcttacctg
    10321 cggtgatttg ggcttaacgc cggggctaaa tcagccccgc attacccagc gtgtggaaca
    10381 ggtgctggct gatgcatttc acgcacaggc tgcggcgctg gtacagggcg cgggtactgg
    10441 cgcgatccgt gcggcactgg cggctttgct caaatcgggg cagcgtcttc tggtgcatga
    10501 cgcgcctgtt tacccgacga cacgggttat tattgagcag atggggctaa cgctcattac
    10561 ggctgatttc aatgatctgt cggcactgaa gcaggttgtt gacgagcaac aacctgatgc
    10621 agcgctggtg cagcatacgc gccagcagcc gcaggaccgc tatgtgctgg cagacgtgct
    10681 ggcaacgttg cgcgcggcag gtgttccagc gttaaccgat gacaactatg cggtgatgaa
    10741 ggtagcgcga atcggctgcg aatgcggggc gaatgtgtcg acattttcct gcttcaagct
    10801 atttgggcca gagggcgttg gtgcggtggt aggcgatgcc gatgttatta gccggattcg
    10861 cgccacgctc tattccggcg gcagtcagat tcagggggcg caggcattag aggtgttgcg
    10921 tggtctggtg ctcgcgccag tgatgcacgc ggtgcaggcg ggggtatctg aacggttgct
    10981 ggctttgctt aacggcggcg cggtggcgga agtgaaaagc gccgtcattg ccaatgcaca
    11041 gtcgaaggta ttgattgtgg agtttcatca gccgattgcc gccagagtgc tggaagaggc
    11101 gcagaaactc ggtgccttgc cttacccggt tggtgcagag tcgaaatatg aaatcccgcc
    11161 gctcttttat cgactttccg gaacgtttcg ccaggcgaat ccgcagttag aacattgcgc
    11221 gattcgcatt aacccaaatc gcggcggtga agagacgata ttgcggatct tgcgtgaaag
    11281 tattgccagc atttaatcgt tgtaaacgat atttttgaaa agaatgcaga aacaaatacg
    11341 ccctcggata actccgaggg cgtcaggcaa ttaaaccggc gttagccgat ttctgtcaat
    11401 agcttactgt gcagtcggac cgcaaccgcc aatgatttta gaaatagaaa ttgccgggtg
    11461 cagcaggtag tcgtaagagc agttcttatc acggttttca atatgaccag tacatgcagt
    11521 taacgtagcc agtactgcgc ctaccagtgc aactttaata aatttgttca tttacattcc
    11581 ctatgtatta tatattaatt aatattcatt gattatatga atataagcag actgctcatt
    11641 aagaacgata caggaatatt caattagcgt cagtgagcga ttgatttaat tttgtaagtc
    11701 gctgtaacgg tgaatataac ccattagcag acaatattta ttaagggggt atgtttttat
    11761 ctgctgaata tataaagaga gttgtaaacg aaaaaggcca gacttctctg gccctggtta
    11821 aattaatggt tagagaacca gttcaattta tcgcgcaggc caacaacccg accaataata
    11881 attagcga
//
LOCUS       DAWDRC010000041        11787 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2821_length_11787_cov_2.546625, whole genome shotgun sequence.
ACCESSION   DAWDRC010000041 DAWDRC010000000
VERSION     DAWDRC010000041.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11787)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_11787_cov_2.546625"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    351..360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    639..648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3612..3621
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3891..3900
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2858 a         2887 c         3067 g         2935 t
ORIGIN      
        1 gtaaatacaa ctctaaacaa tgatctcgtt cttttcttaa taaaattaat tgtgctgccc
       61 gtgaatatta aaaaacaggc ggtgcgtttt ccctggttcc agtaataata agtcgatacc
      121 tgaattaaag gcgttaggcg ggcaactcat tggttcaact gccagtccct gacgttgtaa
      181 tttttcaccg ctataaacct gcacccaggg ttgatcggaa cagaggctca ccgaaagcgc
      241 ctgctgtgga tgggtaatag tcatttccca caaatcatta gcggttttaa aggtgtggtc
      301 gatttttgtc gcggctattt ttttcgcctg cgtgaaattt aaatccagct nnnnnnnnnn
      361 ccagctcatc aacgtggtga agcgttgtcg gattagcgtg ctcatcgatg gcataaactt
      421 ggtttgccgg aagctggaac agatattcat caacagatgt taagttgcag gttaaataag
      481 ggtgaatacc cacaccgtaa ggcgctgcga cagtgccgat attctggctt gcgatctcaa
      541 cggaaagacc cgtgcgggcg ttgagcgaat aaaccacctg cgaagccagc ataaacgggt
      601 agccgtaact gggtggcaga aaggcggtta atgtgacgnn nnnnnnnntg tgacgctggt
      661 ggcgcttaat tcgctgattt gccagtcacg ccaggcgagc agcccatgaa tagcggcttt
      721 tgaaccatgt tcattaatgg gtaactgata ttcctgcccc tgatagcgat aacagccgtt
      781 ggcgatccgg tttggccacg gaataaggac tttgcccaga tgtgccagcg gcatctcttc
      841 gggtttatgc ggaataacta aatgacagcc ctgaaaggtt aattcagcca gacctgcacc
      901 gacggtcaca atggtcgcgt gataatcccc cgcggcaagt gaaacggtct gtccactgca
      961 atgcatgttg gtgatttgca tacgaggtac ctttattgaa taacgacata cccggcgtgc
     1021 agcctgatat gtcgtttttt tagtcagtta tttcgggtta atatccggca gacccgccgc
     1081 aaccgccgga gccaggcctg gcgctaacgg gatacgtggg atcaccagac aatgcagcag
     1141 atgataaata tcctgtttgc cgtcccagac tttggtggtg accttattgt ccgcatccag
     1201 ctcctgccac catgaaccat tttcgtagtc catcaggtat ttgatgcagt agtcccacca
     1261 tgtctgatac caagtttcgt actggcgatc gccagtgacg gtgtagagcg cgtaggccgt
     1321 acccatcgct tcgacgatag gccaacgcac acgttcgcgg accaccggtt ttccttccca
     1381 gtcaacggta taaacaatcc cgtccgcgcc atcgggtgcc caggcatcgc gcacggtggc
     1441 gttaaacaga cctttggcat cttctagcag ccatgctggc ggttgttcgc aacgggcttc
     1501 cagggccgcg tgaatgtgca gcattaaacg gccccattcg atccagtggc ccggagtacc
     1561 tccgaacgcg cggaagcggt gcgccgggtt atctttgttg taatctggca gcggattcca
     1621 ctgggtatcg aagtgttcgt taacgcgata atgattattt ctggcgacgt cgtggataat
     1681 cacggaagcc acgcgaatcg cgcgatccag ccattttttg tcatgggtga cgtcatagac
     1741 aatcaggaag gcttccaccg cgtgcatatt ggcattgccg ccgcgatact cttcggtttt
     1801 gctgaaggct tcatcccagg actccaggca catctgctct tcttcgctcc agaagtattt
     1861 ctcgataatt tcaatggtgt aatcgagcag cttgcgcgct tccggatgac cggtagtgac
     1921 ggcgctggca gcacccagca gagcaaagaa atgttgatag ccctgtttgg aggcatccac
     1981 cacgccctcg tcattcacgc aggcatacca accgccatat tttttatcgc gcagtgcgcc
     2041 attcatggct ttgataccgt gatcaaccaa cgagtaagcg ccaggtcggc ccatcgccgc
     2101 agcaacggaa taaacgtgca acatacgagc tgtgatccac agatgggtgc ccatctcttc
     2161 tttgatttgc cctttattgc ctaaccagcc aaatccggtc ggtactacgg attttttacc
     2221 aaaatcaaag atgcgatcgg tttcctgttc cagccaacgg ttgtgactta aagtgttaaa
     2281 ccatttcatt tttgtatccc catcttaacg gcgtttagcc atcatttcgt cgacgatatc
     2341 gccaagttgt tgtaatttcg gtgcgcaaac gtcacgcagc atcagctcgt tgtctggtaa
     2401 accgacgacc gatgcccaga cggcacgacc agccaggaag cccgatgcac ccgctgtcat
     2461 tgccacgcgt acggcacgcg ggaacagttt ttcgtcgaca ccggaagaga ggatcaccca
     2521 cggcatattg atatggtcat tcagacgttg tgaagcactg agaagctctt gttgcggacc
     2581 tttaccgtaa agcggcattt caactttgta gagatcagcg ccactgtcgc ccagctcttt
     2641 ggctgcatcg atgattgctt gttcgcgatc gaatttatcg ccacgacgcg gtgggcggac
     2701 gactggctca atgatgctta ccagaccgtg tgagtggcac atttcgttaa actctttcac
     2761 catatccagg cgttgctgcg catcttcatc actacgccac agcaccagca gttttaaggc
     2821 tttgccaccg tcttgtttga tttgtagcgg attgattttg ctattaatga ctacgctatt
     2881 taccggaata ccgttgccag gaatgaactc atcggcggcg acaatcatgg cgcaactttt
     2941 ggcaatcgcg ttttgctcaa ccacctggcg gtagcagaat tgttgatcta ccagaatcgc
     3001 cgatgcataa ggtgaaagag tcttcgcggc gttaactttg aaatcagtta aaacgctatc
     3061 ggctactgga gcgggtgcgc cagccgcggc aaacatcatg cgcatggctt cgcgctgatc
     3121 gaccgccagc atggcaaaac cgcccgatgc acgcgtaatg tcgttgatgg tgtacttatt
     3181 cattcattta ttcctttatc cagtcgttac atttttactt tggcagtcat cccggcactg
     3241 acacggacct gctccagaat ggcggaccag tcctggcgac cgcgacccgc tgcgcgcgcc
     3301 tggctataaa cctcccgtga ggcggccccc agtggcatcg gcacatgcaa ctggttggcg
     3361 acatcaaggg cgatgccaag atccttatgg gcaagatcga tcatgaaggc gggagaaaga
     3421 tctccgctga ggactttgtt cggccaggaa gtggtgaagt ggcctttacc ggcggcagta
     3481 ccgctcatta ccttgacggc aacatcgaag ggaagattga gggcttcgca caaaacggcg
     3541 gcttctgccg acaacgcatt gagcgcgatg ctcatgtagt tgttgataag cttaacgcgg
     3601 atccccatgc cnnnnnnnnn ncatgcccgg accgcccgcg ttgatcaact cgctgcccat
     3661 cgccatcagg atcggcgtgg cgcgttcaac ttgttcagca gtgccgccag ccagcagtag
     3721 cagagtaccg gtaatggcat ttgcggaagt acggcctacc ggaacatcca tcatgctgaa
     3781 gcctctggcc tgcatatcag caatcaattt atcggtttgc agcgggtgga tggtcgacat
     3841 atcaatgacc agcgcatcgg aagataagct ctcacagaca ccgttttcaa nnnnnnnnnn
     3901 ccattcggca acatggtaat gataaattcg gcacctttag cggcctgcgc tgggttagca
     3961 gcgggagtcg cccctttgtc gaccagatgc tgtacggctt cggtattcac atcgaagacg
     4021 caaagttgat gcccttgctg caataaattg ctcgccattg gcgaacccat ttgccctaaa
     4081 ccgataaacg cgattgctgc cataactctc tcctgaatac agtgatgtca ctttttgtca
     4141 tttatgacat gccttgcttg tctgtttttg atcgtatttg taatttatcg tcaaaaaatt
     4201 gacagccgtc actttttaaa caattgatga aattaaaatg aacgcatccc aaaatgctta
     4261 aggaatgacc atgattcgtg ttgcttgtgt aggaattacc gtgatggatc gcatctatta
     4321 cgtggaagga ttgccgacgg agagcggtaa atacgtggcg aaaaattata cggaagttgg
     4381 cggcgggcca gcggctacag cagcagttgc ggcggccaga ctaggtgcgc aggtcgattt
     4441 tattggtcgc gtcggtgacg acgacacggg caacagcctg ctggcagagc tggaatcctg
     4501 gggtgttaac acccgttaca ccaaacggta taaccaggcg aaatcttcgc aatccgccat
     4561 catggtggat gccaaaggtg agcgcatcat cattaactat cccagcccag acctactgcc
     4621 tgatgcagag tggttggagg aaattgattt ctctcagtgg gacgtggtgc tggcagatgt
     4681 acgctggcac gacggcgcga aaaaagcctt cactctggcg cggcaggcgg gcgtaatgac
     4741 cgtgctggac ggtgatatta cgccgcagga tatcagcgaa ctggtggcgt taagcgatca
     4801 tgcagctttt tcagaaccgg gtctggcgcg cttaacgggc gtgaaagaga tggcgagtgc
     4861 cctaaaacag gcacaaacgc tcacaaatgg tcatgtctat gtgacccagg gcagcgcagg
     4921 ctgcgactgg ctggaaaatg gcgggcgtca acatcaaccg gccttcaaag ttgatgtggt
     4981 agataccaca ggagcgggtg atgtttttca cggcgctttg gcggtggcgc tggccacagg
     5041 tggggattta gcggagtcag tccgcttcgc cagcggtgta gcggcgttaa aatgcacacg
     5101 tcccggtgga cgagccggga tccctgactg tgatcaaacc cgatcttttt tgtcactttt
     5161 tgtataaaat gccagggtga tggtttttcg aggaattttc atgagcctta ccgaactaac
     5221 cggtaacccg cggcacgacc aactcctcat gctgatcgcc gagcgtgggt atatgaatat
     5281 tgatgagctg gcaaatctgc tggatgtctc cacgcagacg gtgcgccggg atattcgtaa
     5341 attaagcgag caaggactga ttacgcgcca tcacggtggc gcgggtcggg cttccagcgt
     5401 cgttaatacg gcgttcgagc agcgtgaggt ttcgcaaacc gaagaaaaaa aagcgattgc
     5461 cgaagcggtg gcagattata ttcctgatgg ttcaacaata tttatcacca ttggtacgac
     5521 cgttgagcat gttgcccggg cgttacttaa ccataatcat ttgcggataa tcaccaacag
     5581 cctgcgtgtg gcgcatattc tttaccacaa cccgcgcttt gaagtgatgg tgcctggcgg
     5641 aacgctgcgc tcccataaca gtgggatcat tggcccttca gcggcgtcct ttgtggccga
     5701 ttttcgcgcc gattatctgg taacaagcgt aggcgcaatc gagagcgatg gcgcgttgat
     5761 ggagtttgat gttaacgaag ctaacgtggt gaaaacgatg atggcgcacg cgagaaatat
     5821 cctgctggtc gccgatcaca ctaagtatca tgcttcggcg gcggttgaaa ttggtaacgt
     5881 ggcacaggtc actgcgctct ttaccgacga gctgccgccc gctgcgctaa aatcacgctt
     5941 acaagacagc caaattgaaa tcatccttcc ccaggaagac gcgtagaatt ttgtgaccgt
     6001 tgacctggct tcacacctgg ccacatagcc aaacccatcc tttcccgcta cagttaattt
     6061 cttgtggcgc gaaaggaggc aaaaatgctc tatatctttg atttaggtaa tgtgattgtc
     6121 gatatcgact ttaaccgtgt gctgggagcc tggagcgatt taacgcgtgt tccactggct
     6181 acgcttaaga agagtttcca catgggcgag gcgtttcatc agcatgagcg tggggaaatt
     6241 agcgacgaag cgttcgcaga ggcactgtgt catgagatgg ctctaccgct aagctacgag
     6301 cagttctctc acggctggca ggcggtgttt gtcgcgctgc gcccggaagt gatcgccatc
     6361 atgcataaac tgcgtgagca ggggcatcgc gtggtggtgc tttccaatac taaccgcctg
     6421 catactacct tctggccgga ggaatacccg gaaatccgtg atgctgctga ccatatctat
     6481 ctgtcgcaag acttgggtat gcgcaaacct gaagcacgaa tttatcagca tgttttgcag
     6541 gcagaaggtt tttcaccaga cgatacggtc tttttcgacg ataacgccga taatatagaa
     6601 ggagctaatc agctaggcat tactagtatt ctggtgaaag ataaaaccac catcccggac
     6661 tatttcgcga aggtgttatg ctaaaaacca ttcaggacaa agccaggcat cgtacccgtc
     6721 cactatgggc ctggctaaaa ctactctggc aacgcattga tgaggacaac atgacaaccc
     6781 tggcaggtaa ccttgcctat gtgtcgttgc tctcattagt gccgctggtt gccgttgttt
     6841 ttgcgctttt cgccgctttt cccatgtttt ccgacgtcag cattcagttg cgtcacttta
     6901 tttttgccaa ctttctgcct gctactggcg atgttatcca gcgatatatc gaacagtttg
     6961 tcgccaattc caacaaaatg accgccgtcg gggcgtgcgg gctgatcgtc acggcgttat
     7021 tgttgatgta ctccatcgat agcgcgttga ataccatctg gcgcagtaaa cgagcgcgac
     7081 ccaaaattta ctcgtttgcc gtgtactgga tgattttaac gctggggccg ctgttggcag
     7141 gggccagtct ggcgatcagc tcctatctgc tctctctgcg ctgggcgagc gatctcaata
     7201 ctgtcatcga taatgtgttg cgtatttttc cgctgctgtt gtcgtggatc tctttctggc
     7261 tgctgtacag catcgttcct accatccgtg tacctaaccg cgacgcgatt gtcggcgcgt
     7321 ttgtcgccgc gctcctgttc gaagcaggaa agaaaggttt cgcgctttat atcaccatgt
     7381 tcccatcata tcagctcatt tacggtgtgc tggcggtgat ccccattctc tttgtttggg
     7441 tctactggac atggtgtatc gtcttgcttg gcgcggaaat tactgtcact ctcggggaat
     7501 accgcaaatt aaaacaagct gctgaacaag aagaagacga cgaaccatga ttgcattaat
     7561 tcaacgcgta acccgtgcca gcgtcaccgt ggagggagaa gtgacgggtg aaattggcgc
     7621 gggacttttg gtgttattgg gtgtcgaaaa ggatgacgac gaacagaaag caaaccgtct
     7681 gtgcgagcgt gtgctcggct accgcatctt tagcgatgcc gaaggcaaga tgaatctcaa
     7741 cgtgcaacag gcgggcggca gtgtactggt ggtttcccag tttacccttg ccgcagatac
     7801 cgaacggggg atgcgcccaa gtttctccaa aggcgcatca ccggatcgcg cagaggcgtt
     7861 atatgactat ttcgtcgaac gctgccgtca gcaagagatg aacacgcaaa caggacgctt
     7921 cgctgcggat atgcaggtat cgctggtcaa tgatggcccc gtgacattct ggttgcaggt
     7981 atgagccagc ttccagggtt gtcacgggaa acaagagaga gtatcgctat gtatcacctt
     8041 cgggttccac aaacagaaga agaattagag cgttactatc agtttcgctg ggaaatgttg
     8101 cgtaagcccc tgcatcaacc aaaaggttcg gaacgcgacg cgtgggatgc gatggcgcat
     8161 caccagatgg tcgtcgacga gcagggtaat ctggtagcgg taggccgact gtatattaat
     8221 gccgacaatg aagcgtccat tcgctttatg gccgttcatc ctgacgtgca ggacaaaggg
     8281 ttaggcacgc tgatggcgat gaccctggag tcggtggcac gtcaggaagg cgttaagcgc
     8341 gtgacctgta gcgcccgtga agacgcggtg gagtttttcg ccaagctggg gtttgttaat
     8401 cagggagaaa tcaccacgcc aaccaccacg ccgattcgcc attttttgat gataaaacct
     8461 gttgccactc tggatgatat tcttcatcgc ggcgactggt gcgcccagct ccagcaagcg
     8521 tggtatgaac acatcccgct gagtgaaaaa atgggtgtgc gcattcagca atataccggg
     8581 caaaaattta tcaccaccat gccggaaaca ggcaatcaga atccgcatca cacgctgttt
     8641 gccgggagtt tattctcgct ggcaacgctc actggttggg ggcttatctg gctgatgctg
     8701 cgcgaacgcc acctcggcgg aacgattatt ctggcggatg cgcatatccg ctatagcaaa
     8761 ccgattagcg gaaaacccca tgcggtagcc gaccttggtg ccttaagtgg cgatctcgac
     8821 cgtctggcgc gcggacgaaa agcacgggtg cagatgcaag tagaaatctt tggcgacgag
     8881 acgccgggtg cagtgtttga aggcacgtat atcgttctgc ccgcgaagcc atttggcccg
     8941 tatgaagagg gcgggaacga agaagagtag gggcgtttta cttgactgaa aaggttttca
     9001 atctgaaaac cttttctggg ccctggtccg gagttacctt cccgctctcc aggcaacatc
     9061 ccgtagcgcc gatcgcgcag cctcactgat accacctaac tgaaaaaatc cgtggataac
     9121 gccaaggtag cgttggcagg tgcattgcac tccttgccct gtcatgcggt gatacaacgc
     9181 ttcaccttca tcgcaaagtg ggtcgtattc agcggtaatg atatgtacag gtggcaatcc
     9241 attgaaatca ttgcgccaaa tagggctggc ttcaggatgc ctgcgttcaa tgcctggcag
     9301 gtacatctca aagccgctca gtaaagtgtc gcgtgtgatg atgtaatcgt gaccattgcg
     9361 tatgtagctt tcgaaatgcg cagtcgcatc aagcatagga taaattaaga tgagttgtgc
     9421 tggttgccac tcgccagcac gcttcaatcg caaagcagtt accagcgcca gatgccctcc
     9481 cgcactgtca ccggcaagag ttattcgttg tttatcaacg ccgagtcgtt cggcgtgttg
     9541 ccggactaat tctgcacctc gttgtgcatc atcatgggcg gcggggaaag tatgttccgg
     9601 tgccagccga tactggacgg caataatccg acattgcccg taataggcta actggcgcag
     9661 ctggttgtcg tgagttgcga acccgccgct aacaaaacag ccaccgtagt aataaattat
     9721 cgtcgggagt aatgtcgggg cattcagcgg tgacactatt cgtaaggtca tcccttcaag
     9781 ttcgatagtc tgaatatcta cccgcgtttc tgtctcaccc gcaaggacag cgctggcgat
     9841 atagccctct ctgcgctgcg taatactttg ttggcgcgat gaggggcgac ccgcagcgat
     9901 aaactcttcg actaactctg caattccttt ttccagtgcc atagtagaac tcattttgct
     9961 gtatgaatgt acagttttat acacccgttt ttgctggatg gatacaggga aattcacggg
    10021 cgtgaaaaat ctggaaagcg cctcgcaaat gtaaagacaa acccggcaga gacgtggttt
    10081 tgctggagca ctatcagtaa gctagtggtt tcaatggtac gtcaatgacg tacaaaaagg
    10141 attgttactc taagccaatg gcgtataatt atgctcttca tcgaaacgga aatttttact
    10201 gaagatgttc aaaaactgct gaacgacgat gaattcagtc gttttcagtt tttcctggcc
    10261 ttgaatcctg actatggtga ggtaatacct gaaactggtg ggttaagaaa ggttcgatgg
    10321 gtctcaggag gaaaggggaa acgtgctggt gtcagggtta tttactttca tcaggttaaa
    10381 cattatgaga tcagattact gcttatttat cgtaagggta ttaaagatga tctgtctccc
    10441 caggaaaaag caatgctccg gttgttaaat acgaggtggt aaatggataa ggcacttttt
    10501 gagcggttga ctcacagcat ggcccagatg aatgaaatta ttgaaggaac gcgacagcct
    10561 tcacggactt ttgaagttga tgctatgaaa atcaaagaaa tacgccgcgc ttcagggttg
    10621 tcacaatcta aatttgccga tcttatttcc gtaagcgtag acacgttgcg taactgggag
    10681 caggggcgac gttcaccgac cgggcctgca aaggctttgc tgcgagcgat tgccaatgat
    10741 ccgcaacatg tgctgcaagc acttaaccgt tagaggcatg gttatgccat acagaaagca
    10801 ccagcattca gaactcctat gaaagcggca tagtgccgcc tgctcaggag ttttgctatg
    10861 acccgtaatc gtgctgaacg tcgccatcat atggtgcgat taaaaaaaat ccgctgtcgt
    10921 tatcgcactg caggcgatgg aagcaaaaaa gcatcaggta tttgctttcg tactccctgt
    10981 atttgttctt gttggatgtg tgggcatcga cgctatcata atggtccccg cgtatcggaa
    11041 attcgtgcaa aagcacgcta tgcgaattaa gggaaaatga tgaactattt taaggaagca
    11101 catttttaga actcttaaca ggagactaaa aatgtccacg tttaatacgc gtaaagatag
    11161 ggatcgtaat ggtaaagcga agccgcataa gcattcaggg atcccaacac ttacaggtaa
    11221 atggcgtcac ccttcaatgg attgttgggt aaactacagt acaccgggcg atcatgaatg
    11281 gaagcggctc tatactacgc ttcgccgtcg cgcagatgat cgacgccaat gtcatcgggt
    11341 aatgcagggg gatgatccgg aaggaatcgt ctggttcccg gactgttatc cacaaattta
    11401 ctactactaa caattaaaga ccggttggtg cggcactgac cggtcgttat gaatggtaga
    11461 tttcacctta aaccatggtg cggtaaagct gctgactttt tcgggtacac catcctgatg
    11521 aagcaaatga agacccaatc aaaaaatatt cttgaatata aaatatttat agcatccata
    11581 attcatcctt attatttgtt gtttcttttc tgtagatgtg cccccgctta attaaaagta
    11641 ataaaccatt tttgagtaac aattgaaata actgaaactg ttacattatc tttggatgcc
    11701 gtctcgcaaa aacaaaacaa tatgaaaaaa tccgctttcg ttaattaact ttccggcgta
    11761 attttcgcac atacattaat gagtaat
//
LOCUS       DAWDRC010000042        11749 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2832_length_11749_cov_2.088421, whole genome shotgun sequence.
ACCESSION   DAWDRC010000042 DAWDRC010000000
VERSION     DAWDRC010000042.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11749)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2832_length_11749_cov_2.088421"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    294..303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1773..1782
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2941..2950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5888..5897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6252..6261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9615..9624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2644 a         3060 c         3077 g         2908 t
ORIGIN      
        1 gtcagatgcc tcgttggtca gctcaaagcg gtcatgtacc gttgctgcca gtgcggtttc
       61 cacgccttct tcgcccactt tctgtgcaat gcgtttggtg ccgctggcat acagtttggc
      121 tgtgtaagag gtttccgggt cggcagattt acgttcggcg agcagttgtt ccagttgata
      181 caggaacagc cactggtgag cggtatcgcc gaagcagctg ctggtgcctt tgtggcaggt
      241 tggtccgata ggattcgcca gtaccagtag cgtgtcgttg tcgcagtccg gcgnnnnnnn
      301 nnnttgagga aattgcccga cgtttcgcct ttggtccaca gccgttgttt agtgcgcgag
      361 aagaaggtga ctttgccgct ttcgatggtt ttgtctaaag cttccgggtt catatagccc
      421 agcattagca cttcgccgga taccgcgtgt tgcacaatca ccggcataag tccgtcggtt
      481 ttttcccagt ccagttcgcg acgttgttgt tctgttaaca tatcctgatc tccacgccct
      541 gtgttgccag gtacgctttt aattcaccaa tattgattat ttgtttgtgg aatacggaag
      601 ctgccagcgc gccgtcaacg tcggcatcgc ggaaggcttc gagaaagtgt tccatggtgc
      661 ctgcgccacc ggaggcaatc agcgggacgt ggcaaacttc acgcactttt ttcagctgtt
      721 cgaggtcgta accgttacgc acgccgtcct gattcatcat gttgaggacg atttctccgg
      781 ctccacgttt ttgcacttcc tgtacccagt cgagtgtttc ccactgagtg acgcgggtgc
      841 ggctttcatc gccggtatat tgattcacat gatatttacc ggtttcggcg tcgtaccagg
      901 tatcaatgcc gaccacaata cactgcacgc caaagcgatc cgccaggcga gtaattaacg
      961 tcgggtctgc cagtgcaggg gagttaatgg agattttatc cgcgccaaag gaaagaattt
     1021 tcgcggcatc ttccagagac ttaatcccac ccgccacaca aaacggaatg tcgattactt
     1081 ccgccacgcg agatacccag cttttatcta ccacacggcc atcgctggaa gcggtgatat
     1141 cgtagaacac cagttcgtct gcaccttctt cggcgtaacg ttttgccagc ggcacgatat
     1201 cgccaatgat ttcatggttg cgaaactgta cgcctttcac cacctgacca tcacgaacgt
     1261 cgagacatgg aattatgcgt tttgccagca tgcgatagcc tccttcacgg tgaatttacc
     1321 ttccagtaat gcccgaccaa ctattacgcc gcgcacgcca gtacctcgca gggcagcaac
     1381 atcgttaatg tcgccaatgc cgccggagga ctgaaatgcc acctgcgggt atctggcgca
     1441 cacttcttca tataaagaga cgttagaacc tgccagcgtg ccgtcgcgcg agatatcggt
     1501 acatagcaca tgtttcaggc cgacgggaag ataggtttcc accagttgtt ccagtgaaac
     1561 gcccgagttc tcttgccagc cgctgaccgc cacctgcttg ttgccttgct cgtcaatacg
     1621 gacatccagc gccagtacta aggcatcagc accaaaacgt tcaaaccagc ctttcaccat
     1681 ttctggtgat ttcaccgcgg tggagccaac caccacgcgt gcaacgcccg cttccagtaa
     1741 cgtcgctacg tcttcttcac tacgcacacc gcnnnnnnnn nntaatcgcg ctgtttgccg
     1801 taatcgccct gatggagacg caccacggtg ccgtcgatta aatctaatgc cggaataatc
     1861 atcacatctc caggaagttt ttcagcaact tagcgccagc ggcaccagaa cgctccgggt
     1921 ggaactgcac gccgtagaag ttatcttttt gtaccgccgc ggtgaacggt tcgccgtagt
     1981 tacactgggc gatggtccac ggattgaccg gcattgcgta gctgtgaaca aagtaaaagt
     2041 acgcaccgtc ttcaatcccc tgaaacaggc ggttgcctgc ctgcgggtaa acgcggttcc
     2101 agcccatatg cggcagtggc agaccaaagt cggtcatttt cggtacgtat tcgtcgatga
     2161 tacccagcaa atcgacgccg ttgctctctt cgctgcgccg ccccagcagt tgcatcccta
     2221 agcagatgcc cagcaccggt tgggtacagg ctttgatgag atcaaacaac tcgcgctcac
     2281 gcacctggtc catcgccgct tgcgcggtgc caacgccggg taaaaacagt ttatcggcca
     2341 gcaacacgac gtccgggtca cggctaactt tgggttcata accgtgacgc gcaatggcag
     2401 acttcaccga gttcaaattg gcgcagccgg tatcaaggat caccacgttc attacagcac
     2461 tcctttagac gagggcaggg tatcgccttc cacgcggatg gcctggcgca gggtgcgacc
     2521 aaaagctttg aacaggctct ctactcggtg gtgatcgttt ttacctttgg ttttcagatg
     2581 cagggtcacg cccatagtgt atgagagcga acggaagaag tgctcgatca tttcggtgct
     2641 gagatcgccc acgcgctggt aggtaaactc ggccttatat tccaggtgcg ggcgaccaga
     2701 gatatccagc gcgcagcggg caaggcattc gtccatcggc agtacaaaac caaagcggca
     2761 aataccgcgt ttgtcgccaa gtgctatttt taacgcttcg cccagcgcca gaccggtatc
     2821 ttcgacagtg tggtgatcgt cgatatagag gtcgccttta acgttgattt ccatacggaa
     2881 accgccgtga gtggcaatct gatccagcat gtgatcaaag aagccaacgc cggtattaat
     2941 nnnnnnnnnn atcttgctgc caccctcgcg atccagccac acctgaacgt caatctgcgt
     3001 ctctttggtg ttgcgcacca cgtgggcata acgatcgcgt ttcgtgagtt gctcgccaat
     3061 catcggccag ttcagggttt cacggtcgta gcgtaaacca ttaatgccca tgttttccgc
     3121 cagttgaatg tcggtcgcgc gatcgccaat cacataactg ttggcgcgat ccatcgcttg
     3181 ctctgccaga taacgctcca ccagttttac tttcggctta cggcagtcgc actcatcggc
     3241 gggcaggtgt gggcaaatca gcacttcatc aaactgcacg ccttgcgagg tgaagatctg
     3301 catcatcagg ttgtgcgggc catcgaaatc cgcctgcggg aaactttgtg ttccaagacc
     3361 atcctgatta gtgatcatca ccagcttata tcccgctttt tgcagcttca gcagctccgg
     3421 gatcacgcca ggttcaaagg cgagtttgtc aaaacggtcc acctgaaaat cactcggcgg
     3481 ttcgctaatc agggttccat cgcgatcgat aaaaagatac ttctgactca tcaaacttgc
     3541 tccgcacgta aggcgtcaat gacgcactgg ctttcttcac gggttccgac ggtaattcgc
     3601 aggcagccgc ttaaagaggg ttgtttattc tgatcacgta agataatgcc ctgatcccac
     3661 aaagatttaa acactgcgct ggaggcttta aagcgcgcca gaatgtagtt ggtttcggag
     3721 tcgaaaacct gctccacgca ggggatctct ttcagtgcgg caatcaggta ttcgcgttct
     3781 gcaataattt gcgccacccg ttcgcgcatg gcgacgattc cctgcgggct taacgcctgt
     3841 gcggcaatgt cggcaaccgg cgtcgagagc gggtaggggg cgatcacttt catcagcagg
     3901 ttgatgactt cttcgtttgc cagcgtaaat ccgcaacgaa gccccgccag agcaaaagct
     3961 ttcgacagtg tgcgcaaaat agccaggtgc ggatattccg ccagccagcc agccagcgat
     4021 gcctgcgggc aaaactcaat ataggcttca tcggcaacca caatcgcctt atcgcgggtt
     4081 aactccagca gggtgcgaaa atcctgcggg ttgatcagtt gcccggtggg gttgttgggg
     4141 ctgcaaacat agaccacttt tacgccatcc agcttgtcgg aaatgccctg taagtccagt
     4201 tgccagttgt ccagcgtcgg cactgtgcgg cactcgacgc caatggtttc agcgctgacg
     4261 ctgtacatgc cgtacgttgg cgggcagtag aggatggcat ctttacctgg ttcgcaaaaa
     4321 gcgcggatca gcagttcaat accttcgtcc gcgccacggc tgaccagcac ctgttccggt
     4381 tttacgcctg catattgcgc gtaattttca atcaccgctt tcggctggca ttccgggtag
     4441 cggttgagcg tttgctgagt gagctgaaac tccacggctg tggggtattc gttggcgttc
     4501 agccagacgt cgccgttacc gcccagacga cgcgccgact gatacggcgt caggttgcgg
     4561 acgttttcac gcgctaaatc ggtaatagtc acggtgctca tgcttgctcc ttaagggcgt
     4621 taacacgcaa agtaacggca tttttgtggg cggtcaggcg ctcggcggcg gccagtgttt
     4681 caatggtcga cgccagagcg gagaaaccta ctttcgacag ttcctgcacg gtcatgcgct
     4741 tctggaaatc cgccagcccg aggctggaac aggtggcggt gtaaccgtaa gtcggcagaa
     4801 cgtggttggt gccggaggcg taatcacctg cggattccgg tgaccagtca ccaagaaata
     4861 ccgaaccggc gctggtgatg ccatcgacca gttcgcgggc gttgcgggtc tgaatgatca
     4921 ggtgctccgg gccgtactgg ttggagatct ctacgcactg cgctaaatct ttagtcacga
     4981 tcaggcggct ggcgttcagt gcctggcggg cggtttcggc acgcggcagt tctgccagtt
     5041 ggcgttcgac agcttcggct acgcgacgcg ccatatcagc atcaggcgtc agtaaaatca
     5101 cctgagagtc cgggccgtgt tcagcctggg agagcaaatc agaagccacg aaatccggcg
     5161 tagcgccgct gtcagcaatc accagcactt ccgacgggcc tgcgggcata tcgatcgccg
     5221 caccgtccag acgctggctc acctgacgtt ttgcctcggt gacaaaggcg ttacctggcc
     5281 cgaagatttt gtccactttc ggcacggatt ctgtaccaaa cgccagcgcg gcaatggcct
     5341 gtgcaccgcc gacgttaaac acgtcctgca caccgcacag ctgcgccgcg taaagtatct
     5401 catcggctat cggcggcggt gagcacagca ccactttttt acagcccgca atacgcgccg
     5461 gagtcgccag cattaatacc gttgagaaga gcggggcgga gccgccagga atatacaacc
     5521 caactgaagc taccggacgc gtgacttgct ggcaacgtac gcctggctgc gtttctacat
     5581 ctaccggcgg cagtttttgc gcagtgtgga aggtttcaat attctttact gccaccgcca
     5641 tcgcctgttt tagctcgtcg ctcaggcgtt cgctggcggc ggcgatctca tcagcagaca
     5701 ccttcagcgc ggtaaccgtg gttttatcaa acttcgcgct gtattcacgc agggcctcat
     5761 cgccacgcgt tttcacgtta tcgagaatat cgttaacggt gcgggtaatg ctttcagaag
     5821 cggagatcgc cgggcgcatt aacagctggc gttgttgctc cgcagtacag ctattccagt
     5881 caatgatnnn nnnnnnntta aagctcatgg cgatcactcc atcatcttct caatcggcag
     5941 gaccaggatt gaactggcac ccagcgcttt cagtttttcc atcgtttccc agaacagggt
     6001 ttcgctactc accatgtgca tcgctacgcg ctgttgatca cccgccagcg gcagaatggt
     6061 tgggcgttcg gcacctggca gcagggcgat gacttcatcc aggcgttcgg tcggtgcgtg
     6121 catcatgatg tattttgatt cgcgcgcctg gatcacaccc tgaatacggg tcagcagctt
     6181 gtcgatcagt tgctgtttag attcttccat ttcgccatcg cgctggatca ggcaggcttt
     6241 cgagcggtag annnnnnnnn ngacttcgcg caggccgtta gcttccagcg tggcaccggt
     6301 ggaaaccaga tcgcaaatcg catccgccag tccggcgcgc ggcgcaactt cgacagaacc
     6361 gttcagtaag caggatttaa aagagatacc tttctggtcg agataacgct tgagcaggtg
     6421 aggataagag gtggcgatac gtttaccgtt taaggagagc ggaccgtccc aggcttcatc
     6481 aaccggcgtt gccagcgaaa ggcggcagcc gccgaaatcc agacgacgca gggtaaagta
     6541 gcgcggatct tcaccctggg cgcggcggtt aagcagctct tcttccagca cgttttcgcc
     6601 gataatccca aggtctacca cgccgtccat taccagaccg ggaatgtcat cgtcacgcac
     6661 gcgcagaata tcaatcggca tgttttctgc catcgcgatc aggcgctggg tgtgaagatt
     6721 gattttaatg ccacagcgcg ccagtaattc gcgtgagtca tcacttaaac ggccggattt
     6781 ctgcatagct atgcgtaaac gagtgttgtc tgtcattttc ttattcctct ttaaacctgt
     6841 ctgaaccgtt atcaaccgcg caataaaaaa gcccccggaa ggtgatcttc cgggggcttt
     6901 ctcatgcgtt catgcaccac tggaagatct gaatgtcttc cagcacacat cgcctgaaag
     6961 actagtcagg atgatggtga tgatggtggt gtttaaattg aacgcgtgtc ataaaagtct
     7021 ctatgaatgt ttattcaact gatgtctttt aacctaaacc actttcacgt tagaaagcaa
     7081 ggggtttttt atatatttta tggcgatgga tttgtttaat gaatgagcgg ggaaaggagg
     7141 gggctaagag gactgtcctc taacttgaca gcaccaaaaa gagcaaagtc tgatttttcg
     7201 tctcgctgac aatgtcttga tctacaaact aattaataaa tagttaatta acacccatca
     7261 ttgtacaatg aactgtacaa aagaggagat ttacatgcgt acaattagct acagcgaagc
     7321 gcgtcagaat ttgtcggcaa caatgatgaa agccgttgaa gatcatgccc cgatcctcat
     7381 tactcgtcag aatggagagg cttgtgttct gatgtcactc gaagaataca actcgctgga
     7441 agagacggct tatctactgc gttcccccgc taacgcccgg agattgatgg actcaatcga
     7501 tagcctgaaa tcaggcaaag gaacggaaaa ggacattatt gagtgaaact aatctggtct
     7561 gaggaatcat gggatgatta tctgtactgg caggaaacag ataagcgaat tgttaaaaag
     7621 atcaatgaac ttatcaaaga tacccgcaga acaccatttg aaggtaaggg gaaaccagaa
     7681 cccctgaaac ataatttgtc aggtttctgg tcccgacgca ttacagagga gcaccgtctg
     7741 gtatacgcgg ttactgatga ttcactgctc attgcagcat gtcgttatca ttattgaaca
     7801 gattcattca tagaatgaat tgtcagtttt gatacgctag cgtagcctta tagatctaag
     7861 gtgtatcagg agataacgga tgaaaaaggt cgcaattgtc gggttagggt ggttaggtat
     7921 gccgctggcg atgtcacttt cagcgcgagg ctggcaagtc accgggagta aaaccacaca
     7981 agatggtgtc gaagcggccc gaatgagtgg cattgatagc tatctgcttc gcatggagcc
     8041 tgagttagtt tgcgattctg acgatctgga tgccctgatg gatgccgatg cgctggtcat
     8101 tacgcttccg gcacgtcgta gcggccctgg cgatgagttc tatttacaag cggtacaaga
     8161 gttagtggat agcgcgctgg cccatcgtat tccccgcatt atttttacca gctcgacatc
     8221 tgtctatggc gacgcgcaag gcacggtgaa agaaaccacc ccgcgtaatc cggtgaccaa
     8281 cagtggacga gtattagaag aactcgaaga ctggctgcac aatttacccg gtacttcggt
     8341 cgatattctg cgtcttgcgg gcctggtcgg accgggacgt catcccggac gcttctttgc
     8401 cggaaaaacc gcccctgatg gtgaacatgg tgttaattta gtccatttag aagatgttat
     8461 tggcgctatc actctgttgt tacaggcacc taaaggcgga cacatctata atatatgtgc
     8521 gctagctcac cctgcgcgta atgttttcta tccgcagatg gcccgtttac tggggctgga
     8581 acctccgcag ttcagaaata gtctggacag cggcaaaggt aagattattg atggcagtcg
     8641 gatttgtaat gaactgggat ttgaatacca gtatcccgat ccgctggtaa tgccgctgga
     8701 gtaaacgatc acgccagcag gacgccgcat gcactgcaag ggggcgtgta tgaaaccact
     8761 gctggatgtg ttgatgatcc tcgatgcctt agaaaaagaa ggcagttttg cggcggcgtc
     8821 agccaaactc tacaagacgc cgtccgctct gagttacacc gttcacaagc tggaaagcga
     8881 ccttaatatt caattgcttg atcgtagcgg acaccgcgct aaattcaccc gtaccggaaa
     8941 aatgttatta gagaaagggc gggaagttct gcataccgtg cgagaactgg aaaagcaggc
     9001 gatcaaactg catgaaggct gggaaaacga gctggtgatt ggcgtggacg acacgtttcc
     9061 tttttctctg cttgcgcctc tcattgaagc tttttatcaa catcatagcg tgacgcgcct
     9121 gaagtttatc aatggcgtac tcggtggttc ctgggatgcc ctgactcagg ggcgagcgga
     9181 tattatcgtt ggcgcgatgc atgagccacc atcttccagt gagtttggtt tctcgcggtt
     9241 gggcgatctt gaacaagtct ttgcggtcgc gcctcatcat ccgttagccc aggaagaaga
     9301 accgttaaac cgtcgtatca ttaaacgata tcgggcgatt gtggtggggg acactgcgca
     9361 ggcaggcgct tctacggcct cacaacttct cgacgaacaa gaagccatta ccgttttcga
     9421 ttttaaaacc aagctggagc tgcaaattag cggccttggc tgcggctatt taccacggta
     9481 tctggcgcaa cgttttctcg atagtggcgc gttaatcgag aagaaagtgg tcgcccaaac
     9541 tctctttgaa cccgtctgga ttggctggaa tgaacagacc gcagggcttg ccataataat
     9601 ttttctttaa atggnnnnnn nnnncagggc ttgccagcgg ctggtggcga gatgaaattt
     9661 tagcaaatag tgcgatcgcc ggtgtttatg caaaatctga tgacggaaaa tcagccattt
     9721 aaagaaaaat tattatggca agcctctcat tctcttgtca tttccccccc atttaggcac
     9781 aatgcgccgc tgtcaaaaaa tgactaaaaa ccgacgttta attagcgtcg gttatttttt
     9841 gcttcaaacc aatcattcat accaagaggc cgggcttcgt accggataga tatttactaa
     9901 aaatcgacag ttgttgtcgc tgaggaatcc aaaaaaatgg ggcaattttt tgcttacgcg
     9961 acggttatca ccgtaaagga gaatgaccat gtcgcataac gttactccaa acacctctcg
    10021 cgtggaattg cgtaaaacgc ttacgttagt tccggttgta atgatgggtc tagcctatat
    10081 gcagccgatg acgctgtttg atacatttgg tatcgtttca ggcctcacgg atggtcatgt
    10141 gccgacagcc tatgcgttcg cattgattgc gatcctgttt acggctctga gctacgggaa
    10201 gctggttcgc cgctatcctt ctgctggctc tgcatacact tacgcccaga aatccattag
    10261 tccgactgtc ggctttatgg tgggttggtc ttctctgctc gactatctgt tcgcgccgat
    10321 gatcaacatt ctgctggcga aaatctattt tgaagctctg gtgccttcca ttccatcgtg
    10381 gatgtttgtg gtggcgctgg tggcctttat gaccgccttt aacctgcgta gtctgaaatc
    10441 cgtagcgaac ttcaacactg taatcgtcgt gttgcagata gtgctgatcg cggtgattct
    10501 gggcatggtg gtttatggcg tatttgaagg tgaaggcgct ggtacgctgg cgagcactcg
    10561 tccgttctgg tctggcgatg cgcatgttat cccgatgatt accggggcga caatcctgtg
    10621 cttctccttt accggttttg acggcatcag caacctgtcg gaagaaacca aagatgcaga
    10681 gcgcgtgatc ccgcgtgcga ttttcctgac cgcgctgatc ggcggcatga tcttcatctt
    10741 tgcaacttac ttcctgcagc tgtacttccc ggatatctct cgttttaaag atccggatgc
    10801 gtcacagcct gaaatcatgc tgtacgttgc gggtaaagcg ttccaggttg gcgcgctgat
    10861 cttctccacc attaccgtac tggcgtccgg tatggcggcg cacgcaggcg ttgcgcgtct
    10921 gatgtacgta atgggtcgtg acggcgtgtt cccgaaaagc ttcttcggtt atgtacaccc
    10981 gaaatggcgt actccggcga tgaacatcat cctggttggc gcgattgccc tgctggcaat
    11041 caacttcgac ctggtaatgg ctacggcgct gattaacttt ggtgcgttgg tggcgttcac
    11101 cttcgttaac ctgtcggtca tctcgcagtt ctggatccgt gagaagcgta acaaaacgct
    11161 gaaagatcac ttccagtatc tgttcctgcc gatgtgtggt gcgctgaccg ttggtgcgct
    11221 gtgggttaac ctggaagaaa gctcaatggt tctgggtctg atctgggctg ctatcggtct
    11281 gatttacctg gcttgcgtaa ccaagagttt ccgtaacccg gttccgcagt acgaagacgt
    11341 agcgtaatct tacaaaccgg atgacataat catccggtaa atctcaaaaa gccggagttt
    11401 gtcgctccgg cttttttgtt tcagacgatc tcttccgcgt actgccaaag cgcttttaac
    11461 agcgccactt tctctttgtc atcggcatac tgttgtgcca gcatctgcaa ttcatcagcg
    11521 tagccctgca aaaactctgg cgtgaagacc tggcggcggt gctccagcca gcgttgctgc
    11581 tcggcataat ccagcgtccc cgggaagttg cgcgctcgat aattgaacaa cagtttttca
    11641 attcgtttat caacaaaagt gatatccagt gccggtaaat tacgcggctc ggtttccagc
    11701 acaattttca ttgctgcacg atcggcgtca ctgaaaaagc cattataaa
//
LOCUS       DAWDRC010000043        11569 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2894_length_11569_cov_2.188379, whole genome shotgun sequence.
ACCESSION   DAWDRC010000043 DAWDRC010000000
VERSION     DAWDRC010000043.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11569)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2894_length_11569_cov_2.188379"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    515..524
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5778..5787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6028..6037
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8255..8264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9020..9029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9740..9749
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2744 a         2872 c         3146 g         2747 t
ORIGIN      
        1 gtagtcttag ttgtgctgaa cgaaaagcgc acaacgatcc ttcgttcaca gtggggaagt
       61 tttcggatcc atgacgagga gctgcacgat gactgaacag gcaacaacaa tcgatgaact
      121 ggcttttaca aggccgtatg gcgagcagga gaagcaaatt cttactgccg aagcggtaga
      181 atttctgact gagctggtga cgcattttac gccacaacgc aataaacttc tggcagcgcg
      241 tattcagcag cagcaggata tcgataacgg aatgttgcct gattttattt cggaaacagc
      301 ttccattcgt tatgctgact ggaaaattcg cgggattcct gcggacttag aagatcgtcg
      361 cgtagagatt accggacctg tagagcgcaa gatggtgatc aacgcgctca acgccaatgt
      421 gaaagtcttt atggccgatt tcgaagattc actggcacca gactggaaca aagtgatcga
      481 tgggcaaatt aacctgcgcg atgcggttaa cggcnnnnnn nnnncagtta acggcaccat
      541 cagctatacc aatgaagcag gcaaaattta tcagctcaag cccaatccag cggttttgat
      601 ttgtcgggta cgcggtctgc acttgccgga aaaacatgtc acctggcgtg gtgagacaat
      661 ccccggtagc ctgtttgatt ttgcgctcta tttcttccac aactatcagg cactgttggc
      721 aaagggcagc ggtccctatt tctatctgcc gaaaacccag tcctggcagg aagcggcctg
      781 gtggagcgaa gtcttcagct atgcagaaga tcgctttaat ctgccgcgcg gcaccatcaa
      841 ggcgacgttg ctgattgaaa cgctgcccgc cgtgttccag atggatgaaa tccttcacgc
      901 gctgcgtgac catattgttg gtctgaactg cggtcgttgg gattacatct tcagttatat
      961 caaaacattg aaaaactatc ccgatcgcgt cctgccagac agacaggcag tgacgatgga
     1021 taaacccttc ctgaatgctt actcacgcct gttgattaaa acctgccata aacgcggtgc
     1081 ttttgcgatg ggcggtatgg cggcatttat tccgagcaaa gatgaagagc gcaataacca
     1141 ggtgctcaac aaagtaaaag cggataaatc gctggaagct aataacggtc acgatggcac
     1201 atggattgcc cacccaggcc ttgcggatac ggcaatggcg gtattcaacg acattctcgg
     1261 ctcccgtaaa aatcagcttg aagtgatgcg cgaacaagac gcgccgatta ctgccgatca
     1321 gctgctggca ccttgtgatg gtgaacgcac cgaagaaggt atgcgcgcca acattcgcgt
     1381 ggctgtgcag tacatcgaag cgtggatctc tggcaatggc tgtgtgccga tttatggcct
     1441 gatggaagat gcggcgacgg ctgaaatttc ccgtacctca atctggcagt ggatccatca
     1501 tcaaaaaacg ttgagcaatg gcaaaccggt gactaaagcc ttgttccgcc agatgctggg
     1561 cgaagagatg aaagtcattg ccagcgaact gggcgaagaa cgtttctccc aggggcgttt
     1621 tgacgatgcc gcacgcttga tggaacagat caccacttcc gatgagttaa ttgatttcct
     1681 gaccctgcca ggctaccgcc tgttagcgta aaccaccaca taactatgga gcatctgcac
     1741 atgaaaaccc gtacacaaca aattgaagaa ttacagaaag agtggactca accgcgttgg
     1801 gaaggcatta ctcgcccgta cagcgcggaa gatgtggtga aattacgcgg ttcagtcaat
     1861 ccggaatgca ccctggcgca actgggcgcg gcgaaaatgt ggcgtctgct gcacggcgag
     1921 tcgaaaaaag gctacatcaa cagcctcggt gcactgactg gtggtcaggc gctgcaacag
     1981 gcgaaagcgg gtattgaagc agtctatctg tcgggatggc aggtagcggc ggacgctaac
     2041 ctggcggcca gcatgtatcc ggatcagtcg ctctatccgg caaactcggt gccagcggtg
     2101 gtggagcgga tcaacaatac tttccgccgt gcggatcaga tccaatggtc cgcgggcatt
     2161 gagccgggcg atccgcgcta tgtcgattac ttcctgccga tcgtcgccga tgcggaggcc
     2221 ggttttggcg gtgtactgaa tgcctttgaa ctgatgaaag cgatgattga agccggtgca
     2281 gcggcagttc acttcgaaga tcagctggca tcagtgaaga aatgtggtca catgggcggc
     2341 aaagttttgg tgccaactca ggaagctatt cagaaactgg tcgcggcgcg tctggcagct
     2401 gatgtgacgg gcgttccaac cctgctggtt gctcgtaccg atgccgatgc ggcggatctg
     2461 attacctccg attgcgaccc atatgacagc gaatttatta ctggcgagcg taccagtgaa
     2521 ggcttcttcc gtactcatgc gggtattgag caagcgatca gccgtggcct ggcgtatgcg
     2581 ccatatgctg acctggtatg gtgtgaaacc tccacgccgg atctggaact ggcgcgtcgc
     2641 tttgcacaag ccatccacgc gaaatatccg ggcaaactgc tggcttataa ctgctcgccg
     2701 tcgttcaact ggcagaaaaa cctcgacgac aaaactattg ccagcttcca gcagcagctg
     2761 tcggatatgg gctacaagtt ccagttcatc accctggcag gtatccacag catgtggttc
     2821 aacatgtttg acctggcaaa cgcctatgcc cagggcgagg gtatgaagca ctatgttgag
     2881 aaagtgcagc agccggaatt tgccgccgcg aaagatggct ataccttcgt atctcaccaa
     2941 caggaagtgg gtacaggtta cttcgataaa gtgacgacca ttattcaagg tggcacgtct
     3001 tcagttaccg cgctgaccgg ctccaccgaa gaatcgcagt tctaagcaac cgataaacgt
     3061 tgctgactgt aggccggata aggcgttcac gccgcatccg gcaatcagcg cctgatgcga
     3121 cgcttgcgcg tcttatccgg caattctctg ctcccgacga gggcaacaaa tgccgcgtgg
     3181 cctggaatta ctgattgcac aaaccatttt gcaaggtttc gacgctcagt atggtcgatt
     3241 cctcgaagtg acttccgggg cgcagcagcg tttcgagcaa gccgactggc atgctgtcca
     3301 gcaggcgatg aaaaaccgta ttcatcttta cgatcatcac gtgggtctgg tcgtggagca
     3361 actgcgctgc attactaatg gccaaagcac ggacgcggca tttttactgc gcgttaaaga
     3421 gcattacacc cggctgttac cagattaccc gcgtttcgag attgcggaga gctttttcaa
     3481 ctccgtgtac tgtcggttat ttgaccaccg ctcgcttact cccgagcggc tttttatctt
     3541 tagctcccag ccagagcgcc gttttcgtac cattccccgc ccgctggcga aagactttca
     3601 ccccgatcac ggttgggaat ctctgctgat gcgcgttatc agcgatctgc cgctgcgtct
     3661 gcgttggcag aataaaagcc gtgacattca ttacatcgtt cgccatctga cggaaacgct
     3721 ggggacagac aacctcgcgg aaagtcattt acaggtggcg aacgaactgt tttaccgcaa
     3781 taaggcagcc tggctggtgg gcaaactaat aacgccttcc ggcacgttgc catttttatt
     3841 gccgatccac cagacggacg acggcgagtt atttattgat acctgcctga cgacgaccgc
     3901 cgaagcgagc attgtttttg gctttgcgcg ttcttatttt atggtttatg caccgctgcc
     3961 cgcagcgctg gtcgagtggc tacgggaaat tctgccaggt aaaaccaccg ctgaattgta
     4021 tatggctatc ggctgccaga agcacgctaa aaccgaaagc taccgcgaat atctcgttta
     4081 tctacagggc tgtaatgagc agttcattga agcgccgggt attcgtggaa tggtgatgtt
     4141 ggtgtttacg ttgccgggtt ttgatcgggt atttaaagtc atcaaagaca ggttcgcgcc
     4201 gcagaaagag atgtctgccg ctcacgttcg tgcctgctat caactggtga aagagcacga
     4261 tcgcgtgggc cgaatggcgg acacccagga gtttgaaaac tttgtgctgg agaagcggca
     4321 tatttccccg gcattaatgg aattactgct ccaggaagca gcggaaaaaa tcaccgatct
     4381 cggcgaacaa attgtgattc gccatcttta tatcgaacga cggatggtgc cgcttaatat
     4441 ctggcttgag caagttgagg ggcagcaatt gcgtgatgcc attgaagagt acggcaacgc
     4501 cattcgccag cttgccgctg ccaacatctt ccctggggat atgttattta aaaactttgg
     4561 cgtcacccgt cacgggcgcg tggtgtttta tgattacgac gaaatttgct acatgacgga
     4621 agtgaatttc cgcgacatcc cgctgccgcg ctacccggaa gacgaacttg ccagcgaacc
     4681 gtggtacagc gtctcgccgg gcgatgtttt cccggaagag ttccgtcact ggttatgtgc
     4741 cgacccgcgt attggtccgc tatttgaaga gatgcacgcc gacttgttcc gcgctgatta
     4801 ctggcgcgca ctacaaaacc gcatacgtga agggcatgtg gaagatgttt atgcgtatcg
     4861 gcgcaggcag cggtttagcg ttcgattcgt ataaatcaga ggcgaaaaag tcagcgtatt
     4921 ccaccgtacg acagcgtcac ttccttcgcc gctttaatca ccatcgcgcc aaactcggtc
     4981 acgcggtcat cggtaatacg tgaaatcggt ccggaaattg aaattgcggc aaacggttcg
     5041 cggtgctcat cgaaaataca cgctgcaagg caacgcagcc ccagcgcatg ttcctcatcg
     5101 tcaaatgaat aaccccgttt gcgcgtttgg gcgagatctt cttttaaatg cacaggagac
     5161 accagcgtcg cgtgggtata ggcatgtaac catttgcggt gcagcagctt cgtcacctgt
     5221 tcttcgctca gttgggccag aaaggcttta cccgcaccgg aagcgtgcat cggcaattta
     5281 ccgccgatag gcgcggacat tcgcatcagg tgagtacact gtacctggtc gataataatc
     5341 gcttcgtgat cgctttgatc aagcaccgcc atattgaccg tttcgccaga ctcttccatt
     5401 agattgcgca ggatagggtg aacaatcgct aacaaattac ggctctggag aaagctgctg
     5461 ccgaccataa aggcgtgtgc gccgatcgcc cagtgaccca gttcgccgac ctgacggaca
     5521 aagccctgct gttgcatcga ggttagcagg cggtgggtcg tggaattggg taacccggct
     5581 tgctgtgcca gttccgtgag tgccacactg ccattggatt cggcaatcca ctccagtaat
     5641 ttcaggccac gcgttaaaga ctgaacctgt ccagtcgctg gtgcggtggc aacggcgggt
     5701 tttctgccgc gttttgcggg aatgggtgcg accatgacag tctccttttt tctgtatcgt
     5761 ggaaatcatt ttcatttnnn nnnnnnngcg aatgcaatag ttgctgaaca gatccgatga
     5821 gttaatgttg aacaaatctc atgttgcgtg gtggtcgctt ttaccacaga tgcgtttatg
     5881 ccagtatggt ttgttgaatt tttattaaat ctgggttaag cgtgtcggga gcaagtgtga
     5941 gcagcaaagt ggaacaactg cgtgcgcagt taaatgaacg tattctggtg ctggacggcg
     6001 gtatgggcac catgatccag agctatcnnn nnnnnnnatc gactgaacga agccgatttt
     6061 cgaggtgaac gctttgccga ctggccatgc gatctcaaag gcaacaacga cctgctggtg
     6121 ctcagcaaac cggaagtgat cgccgctatc cacaacgcct actttgaagc gggcgcggat
     6181 atcatcgaaa ccaacacctt caactccacg accattgcga tggcggatta ccagatggaa
     6241 tccctgtcgg cggaaatcaa ctttgcggcg gcgaaactgg cgcgcgcctg tgctgatgag
     6301 tggaccgcgc gcacgccgga gaaaccgcgc tacgttgccg gtgttctcgg cccgaccaac
     6361 cgcacggcgt ctatatctcc ggacgtcaac gatccggcat tccgcaacat cacttttgac
     6421 cagctggtgg cggcttatcg agaatccacc aaagcgctgg tggaaggtgg cgcggacctg
     6481 atcctgattg aaaccgtttt cgacaccctt aacgccaaag cggcggtctt tgcggtgaaa
     6541 acggagtttg aagcgctggg cgttgaactg ccgattatga tctccggcac catcaccgac
     6601 gcctccgggc gcacgctctc cgggcagacc actgaagcgt tttacaactc cctgcgccac
     6661 gccgaagcgc tgacctttgg cctgaactgt gcgctggggc cagacgaact gcgccagtac
     6721 gtacaggaac tgtcgcgtat tgcggaatgc tacgtcaccg cccacccgaa cgccgggctg
     6781 cccaacgcct ttggtgaata cgatctcgac gccgacacga tggcaaaaca gatacgtgaa
     6841 tgggcagaag caggctttct caatatcgtc ggcggctgct gcggcaccac gccacaacat
     6901 attgcagcga tgagtcgtgc agtagaagga ttagcgccgc gcaaactacc ggaaattccc
     6961 gtagcctgcc gcttgtccgg cctggagccg ctgaacattg gcgaagatag cctgtttgtg
     7021 aacgtgggtg aacgcaccaa cgtcaccggt tccgctaagt tcaagcgcct gatcaaagaa
     7081 gagaaataca gcgaggcgct ggatgtggcg cgtcagcagg tggaaaacgg tgcgcagatt
     7141 atcgatatca acatggatga agggatgctc gacgccgaag cggcgatggt gcgttttctc
     7201 aatctgattg ccggtgaacc ggatatcgct cgcgtgccga taatgatcga ctcctcaaaa
     7261 tgggacgtta ttgaaaaagg tctgaagtgt atccagggca aaggcattgt taactctatc
     7321 tcgatgaaag agggtgtcga cgcctttatc catcacgcga aactgttgcg tcgctacggt
     7381 gcggcagtcg tggtaatggc ttttgacgaa cagggccagg ccgatacccg cgcacggaaa
     7441 atcgagattt gccgtcgggc gtacaaaatc ctcaccgaag aggttggctt cccggctgaa
     7501 gatatcatct tcgacccgaa catcttcgcg gtcgcaactg gcattgaaga gcacaacaat
     7561 tacgcgcagg actttatcgg cgcgtgtgaa gacatcaaac gtgaactgcc gcacgcactg
     7621 atttccggcg gcgtttctaa cgtttctttc tcattccgtg gcaacgatcc ggtgcgcgaa
     7681 gccattcacg cggtgttcct ctactacgct attcgcaatg gcatggatat ggggatcgtc
     7741 aacgccgggc aactggcgat ttacgacgac ctgcctaccg agctacgcga cgcggtggag
     7801 gatgtgatcc tcaaccgtcg cgacgatggc accgagcgtt tactcgagct tgccgagaaa
     7861 tatcgcggca gcaaaaccga cgacaccgct aacacccagc aggcggagtg gcgctcgtgg
     7921 gaagtgaata aacgtctgga atactcgctg gtcaaaggca ttaccgagtt tatcgagcag
     7981 gataccgaag aagcccgcca gcaggctacg cgtccgattg aagtgattga aggcccgttg
     8041 atggacggca tgaacgtggt cggcgacctg tttggcgagg gcaaaatgtt cctgccgcag
     8101 gtggtgaaat cggcgcgcgt aatgaagcag gcggtggcgt atctcgaacc gtttattgaa
     8161 gccagcaaag agcagggtaa aaccaacggc aagatggtga tcgccaccgt gaaaggcgat
     8221 gtccacgaca tcggtaaaaa tatcgttggt gtggnnnnnn nnnngcaatg taacaactac
     8281 gaaattgtcg atctcggcgt gatggtgcct gcggaaaaaa ttctccgtac cgctaaagaa
     8341 gtgaatgctg atttgattgg cctttcgggg ctgatcacgc cgtcgctgga cgagatggtt
     8401 aacgtggcga aagagatgga gcgtcagggc ttcaccattc cgctattgat tggcggcgcg
     8461 accacctcaa aagcgcacac ggcggtgaaa attgagcaga actacagcgg cccgacggtg
     8521 tatgtgcaga acgcttcacg caccgttggt gtggtggcgg cgttgctttc cgacactcag
     8581 cgtgatgatt tcgttgctcg tacccgcaag gagtacgaaa ccgtgcgtat tcagcacgga
     8641 cgaaagaaac cacgcacacc accggtcacg ctggaagcgg cgcgtgataa cgacttcgct
     8701 tttgactggc aggcttacac gccgccggtg gtgcaccgtc tcggtgtgca ggaagtcgaa
     8761 gccagcatcg aaacgctgcg taattacatc gactggacgc cgttctttat gacctggtcg
     8821 ctggccggga agtatccgcg cattctggaa gatgaagtgg taggtgttga ggcgcagcgg
     8881 ctgtttaaag acgccaacga catgctggat aaattaagcg ccgaaaaaat gctgaacccg
     8941 cgtggcgtgg tgggcctgtt cccggcaaac cgtgtgggtg atgacattga aatctaccgt
     9001 gacgaaacgc gtacccatgn nnnnnnnnna gccaccatct gcgccaacag accgaaaaaa
     9061 ccggcttcgc taactactgt ctcgctgact tcgttgcgcc gaaggtttcc ggcaaagcgg
     9121 attacatcgg cgcatttgcc gtgactggcg ggctggaaga ggacgcactg gctgatgcct
     9181 ttgaagcaca gcatgatgat tacaacaaaa tcatggtgaa agcgcttgcc gaccgtctgg
     9241 cggaagcctt tgcggagtat ctccatgagc gtgtgcgtaa agtctactgg ggctatgcgc
     9301 cgaacgagaa cctcagcaac gaagagctga tccgcgaaaa ctaccagggc atccgtccgg
     9361 caccgggcta ccctgcctgc ccggaacatt cggaaaaagc cacaatctgg gagctgctgg
     9421 aagtggaaaa acataccggt atgaaactca cggaatcctt cgccatgtgg ccgggtgcat
     9481 cggtttcggg ttggtacttc agtcatccag acagcaagta ttacgcggtg gcgcaaattc
     9541 agcgcgatca ggttgaagac tatgcccgcc gtaaaggaat gagcgttacc gaagttgagc
     9601 gttggttggc accgaatctg gggtatgatg ccgagtaagt gataacaccg gggtatttgc
     9661 cccggttttt aatgtcataa ataatggatt attgaacatg ataaaaattg atgaaataga
     9721 ttttttcgct ttcgaaggan nnnnnnnnnt tataatgatt ggataattaa tataaaaaca
     9781 ggtgtggagt gggtatttta tgatggtcgt tattggttag attttcgtaa atccaaagag
     9841 ggtataaggt gtatctcatg cagtattctg tatcacgata aaagtaaagg gcgtatcgat
     9901 gtcttgttta gtgaaggtat agctgacaga ggtttaaaaa atacgggtgg agtgttaatc
     9961 caaaaacgcc cgcaattata ataattcctg gtaatatttg ttttcgtaaa aaggatttac
    10021 tttatgcaac gtcaatataa aaaatacatt cttctttttt ggggtgttat ggatgttatc
    10081 gcgatagcca catatctgtt ctactcgctt ggtaatggtc gtatcccttt ttattccgat
    10141 atcgttcatt cactttcttt acttaaggat atcggtgttg aaggcggttt ttacgcttat
    10201 gcagtcgcca ctgttacgct acaaatagta ttattgttct ctttattctt ttctgcgcaa
    10261 tgttttttaa gacaaaaatt atttagtttc tcttttttgg cgattcagga gtttatgcgt
    10321 tttgctaccg taagctgttc aatctccgta ataccgttgt tacttaatta ttttcattca
    10381 cagaatctgg tcttaaatat ttcgttgttt atcttctcag aggtcacaaa agtgacaacg
    10441 attatctggt gtaaacgaca ggggaaagtg tgacagaaat agcaacagat acaggtggat
    10501 ttttatataa atgccttttt aaatgctttc ttgtataaat ctcttatact gaaagtacat
    10561 acaagagaaa tgcctggata aaaacaacgt gattagaaaa atcttaacag gaaaatgcat
    10621 aacaaaaagg agaacctgat tccgtgctaa ctttgcttca cctgctttct gccgtcgccc
    10681 tgctggtctg ggggactcat attgttcgaa caggcgtaat gcgcgtcttc ggcgcgcgtc
    10741 tgcgtactgt ccttagccgg agcgtcgaaa aaaagccgct cgccttttgc gcggggatcg
    10801 gcgttaccgc actggtgcag agcagtaatg ccaccaccat gctggtgacc tcgttcgtcg
    10861 ctcaggatct ggttgctctc gcgcctgcgc tggtgattgt gctgggtgca gatgtcggga
    10921 cggcgctaat ggcgcgtatt ctcaccttcg atttatcctg gctgtcaccg ttacttattt
    10981 ttatcggcgt gatttttttc ctcggacgca aacagtcacg cgccgggcaa ctgggccgcg
    11041 tcggtattgg tcttgggctg attttactgg cgctggagtt gattgtgcag gccgtaacgc
    11101 cgatcactca ggcaaacggc gttcaggtga tctttgcctc gctgactggc gacattttgc
    11161 tggatgcgct gattggtgcg atgttcgcca ttatcagcta ctccagcctt gctgctgtac
    11221 tgctgaccgc gactttgacc gccgcaggca ttatctcttt cccagtggcg ctttgtctgg
    11281 tgattggggc aaacctcggt tccggcctgc tggcgatgct caacaacagt gccgccaatg
    11341 ccgcagcccg ccgtgtcgcg ctgggtagcc tgctgtttaa gctggtgggt agcctgatta
    11401 tcctgccgtt tgttcatttg ctggcagaga caatggggaa gttgccattg ccaaaagcgg
    11461 aattggtgat ctatttccac gtcttctaca accttgtacg ctgcctggtc atgttgccat
    11521 tcgttgaccc gatggcacgg ttttgcaaaa cgattattcg cgatgaacc
//
LOCUS       DAWDRC010000044        11486 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2916_length_11486_cov_2.556207, whole genome shotgun sequence.
ACCESSION   DAWDRC010000044 DAWDRC010000000
VERSION     DAWDRC010000044.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11486)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11486)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2916_length_11486_cov_2.556207"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8772..8781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9835..9844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2703 a         2931 c         2965 g         2867 t
ORIGIN      
        1 gaaagttgct ttttgaagcg acgttcgtca cgattgaaaa atgttgtgat accaatcaga
       61 aaggcgatga cacccacgcc ctgggccagc caatacgcgg tcatgataaa tccttagcag
      121 gtatggaaaa gcaaacggcg cttcacatta tgaaacgccg ttttttatta acaactcatt
      181 tcgactttat agcgttacgc cgcttttgaa gatcgccagt tcgcggaagt cgttacgctc
      241 gttgcaggtt tgtttaccat tggcaaactc aacgatggtg tcgataaatt cttccagcaa
      301 ctgcggcatc gctttaccgt ggatcagctg acccgcgtca aagtcgatcc agtgtttttt
      361 cttcgccgcc agttcactgt tggtggcgat tttcaccgtc ggcacaaatc caccatacgg
      421 cgtaccacga ccagtactga acagcaccat atggcagccc gcacccgcca gggcgctggt
      481 cgctacggca tcgttacccg gcgcacttaa caagttcagc cccggcgttt tcagacgctc
      541 gccgtaacgc agcacgtcaa ccacgacact ggtacccgct ttctgggtac agccaagtga
      601 tttgtcttcc agcgtggtga taccgcctgc tttgttcccc ggtgatgggt tttcgtagat
      661 cggctggtca tgggcaataa agtactgctt gaagtcattg accatggtga ccagtttttc
      721 aaacgttgct tcgtcgcgac aatggtccat cagcaactgc tctgcgccaa acatctccgg
      781 cacttcggtc agtacggtag taccgccgtt agcaatcacg tagtcagaga aacgccccag
      841 catcgggtta gcagtaatac cagaaagacc gtcagaacca ccgcactcca gaccaaactt
      901 cagttcgctg agtttgcccg gctcgcgttt atcgttgcgc atcacgctat acagctgatg
      961 caaatgcgcg atcccggcct cgatttcatc atcctgctgt tggcagatca tgaaatgaac
     1021 gcgttcagga tcgatatcgc ccaacgtttc acggaatgcg gcaacctggt tgttttcaca
     1081 gcccagacca atcaccagca ctgcgcccgc gttcgggtgg cgcaccatgt tttgcagcat
     1141 ggtgcgagta ttgatgtgat cgtcgcccag ttgtgagcag ccgtaggtgt ggctgaagag
     1201 gaacacgccg tcggtacctt cagcgttgtt ggtctctttc aggaaacgat tttggatctg
     1261 ccgcgcgatg ccgttgacac agcccacggt tggcaggatc cacagctcat tacgcacccc
     1321 gacatcgccg ttagcgcgac gatagatctg cacttcacga tctgccgctt gcgcaggcag
     1381 atcctgaaaa tcaggttgat agcgatactg atccagatcg ctcagattcg tgcgcgtatt
     1441 gtgggcgtga acgtgttccc ccgccgcaat atccgccaat gcataaccaa tcggcaggcc
     1501 atatttaatg acgttggccc cttttgcgat atccgttaac gcaaatttat gtccacgagc
     1561 aacatcctgg cgcaccgtaa cagtctggtt atcgacactg acttctgtgc cttcagccaa
     1621 atccgctaaa gcaaccgcga cgttatccag cgcatggatc ttgatgtatt gcatatcaac
     1681 cccagacctt agttcagttc aatggcgaag tagtcacgcg cattgttaaa gcaaatattt
     1741 ttcaccatct cgcccagcag gttgatgtcc gccggtgctt cgcctgcttc cacccagcga
     1801 ccgatcatct ggcacagaat gcggcggaag tattcgtgac gggtgtatga caggaagcta
     1861 cggctgtcag tcagcatgcc gacaaagcgg ctcagcagac cgagctgcgc cagttgggtc
     1921 atctgacgtt ccataccgtc tttctggtcg ttaaaccacc agccggaacc gaactgcatt
     1981 ttgcccggca taccttcgcc ctggaagtta ccgatcatgg tgcccagcac ttcgttatcg
     2041 cgcgggttca ggcagtagag aatggttttc ggcagcaggt tttcttcatt ctgcttgctc
     2101 agcagcttag acagctcttc cgccatcgga cggtcgttga tggagtcaaa gccgacatcc
     2161 ggccccagca gtttgaactg acgcaggttg ttattacgca gtgcgccaat gtggtactgc
     2221 tgtacccatc cgcgacgtgc atattcggca ccaaggaaca ccagcaccgc agttttgaac
     2281 tgtgccactt cgtgctcgct cagggtttcg cctgccagac ggcgcgcgag gatgctgtcc
     2341 agttccgctt cgttcgcttc agcaaacatc accacatcca gcgcgtggtc agacacttta
     2401 cagccgtgag cggcgaagtg atccagacgt ttagtcaggg cagtttgcag gtcagcaaag
     2461 cggcgaatgt cggtatcgga aacttcgccc agcttcgcca tgtagtcgtt aaaggtcgcc
     2521 tgttcaatgt taaaggcttt gtccggacgc cagctcggca gcactttaat ggtgaaagag
     2581 ccgtctttgg cgatctctgc gtgatgctcc agagaatcga tcgggtcatc ggtggtgccg
     2641 accattttca cgttcatctg ctgcatgatg ccgcgtgcag agaaattatc ctgcgccagc
     2701 agttcgttac attcgttcca gatttcatcg gcagttgacg gagaaagcaa tttgccagtg
     2761 ataccaaacg gacgacgcag ttcgaggtgc gtccagtggt ataacgggtt gccgatagtg
     2821 tgcggaacag tcgccgccca ggcgtcaaat ttttcacggt cagacgcatc accggtgcac
     2881 agacgctcgg ccacaccgtt ggtacgcata gcgcgccatt tgtagtgatc gcccttcaac
     2941 cagatgtcat acaggttttt aaaacgatag tcttccgcaa tctgctgcgg cggcaaatgg
     3001 caatggtaat cgaaaatcgg ctggtctttt gcgtagtcgt gatacagacg gcgggcaaat
     3061 tcagtatcta acaggaaatc ttcagtcata aacggagtca ttttcgtctt cctctcaacg
     3121 agtgagctag cttgcttatg gtgcgatgct gacaaagtta tcacaccaat ttccagagtc
     3181 cgaagatatt ttcgtgagtt agatcaataa acgtagttaa aaaaattact ctcaaagtgg
     3241 taaatctcgc tgcaggccgc gccagtactg gcctcgccgt cgtcaggtaa tgtccctaca
     3301 aatattcgca catttgtgat ggctctcacc ttttaaagtt gtatgacaag ttatctttct
     3361 gccgtcgcaa atcataagtc gacggaatgc aaattgccga ttcattcatt tgttagatga
     3421 atcgggttaa ccggtacgga agccgaatta gcacgaaact ttcatggcaa cgttcggggc
     3481 gtgccggttt tttttcggtt acccggtcgt aactaacatc ttcagcctct ggcgggatga
     3541 tggccgcgct tcctgcggat ataacaaaac gatgaggttt tacatgcgta aaattaaagg
     3601 gttacgttgg tatatgatcg cactggtgac gctcggcacc gtgcttggtt acctgacgcg
     3661 taacactgtg gcggcagctg cgccaactct gatggaagag ttaaacatct ccacccaaca
     3721 gtattcctat atcatcgcag cgtattctgc tgcttatacg gtcatgcaac cggtagcggg
     3781 ttatgtactg gatgtgctgg gtacgaaaat cggttatgca atgtttgctg tactgtgggc
     3841 cgtgttctgt ggtgcaactg cgctggcagg tagttggggt ggcctggcgg ttgctcgtgg
     3901 tgcggtcggt gccgcggaag ccgcgatgat tccggcgggt ctgaaagcca gctccgaatg
     3961 gttcccggcg aaagagcgtt ccatcgcggt aggttacttt aacgtaggtt cttcgattgg
     4021 tgcgatgatt gcgccgccgc tggtggtatg ggcaatcgtg atgcacagct ggcagatggc
     4081 atttatcatc tccggtgcgt tgagctttat ctgggcgatg gcatggctga ttttctataa
     4141 acatccgcgc gaccagaaac atctgaccga tgaagaacgc gactacatta ttaatggtca
     4201 ggaagcccag caccaggttg acacagcgaa gaaaatgtcc gttggtcaga tcctgcgcaa
     4261 ccgtcagttc tggggtatcg cgctgccgcg cttcctggca gaaccggcat ggggtacttt
     4321 taacgcgtgg atcccgctgt tcatgtttaa agtttacggc tttaacctga aagagatcgc
     4381 gatgtttgcc tggatgccga tgctgtttgc tgacctcggt tgtatcctcg gtggttacct
     4441 gccgccgctg ttccagcgtt ggtttggtgt gaacctgatc gtttcccgta agatggtcgt
     4501 aacgctgggt gcggtgctga tgattggccc gggtatgatc ggtctgttca ccaacccgta
     4561 tgtcgcgatt atgctgctgt gtatcggtgg ttttgctcac caggcactgt ctggcgcact
     4621 gattacgctc tcgtctgacg tgttcggtcg taacgaagtg gcgacggcga acggtttaac
     4681 cgggatgtcc gcatggctgg caagtacgct gtttgcactg gtagtcggtg cactggctga
     4741 caccatcggc ttcagcccgc tgttcgcagt tctggcagtg ttcgacctgt taggtgcgct
     4801 ggttatctgg accgtgttgc agaacaaacc ggcgattgag gtggcgcagg aatcacataa
     4861 cgatcccgca ccgcaacatt aaggcttcgt gtaagtttta gtttcccttc aaagccgcct
     4921 tctcaggcgg ctttttcatc actgcgagta gagctaaact cgcaaaagtg gtataacaaa
     4981 tatagtctgc cgtatcatgc ctggagcgca tatggaaatc actgaaccac gccgtttgta
     5041 tcaacaactt gccgctgatc tgaaagagcg catcgaacag ggcgtctatc tggtgggtga
     5101 taaactgcct gccgaacgct ttattgccga tgaaaaaaac gtcagccgca ccgtggttcg
     5161 tgaagccatc atcatgctgg aagttgaagg ctatgtcgaa gtgcgtaaag gttcaggtat
     5221 tcatgtggtt tccaaccagc ctcgccatca gcaggcggct gacaataata tggaattcgc
     5281 caattacggt ccgtttgagt tgctccaggc tcgccagctc atcgaaagta atattgccga
     5341 gttcgcggca actcaggtaa cgaaacagga catcatgaaa ttgatggcca tccaggagca
     5401 ggcgcgcggc gaacaatgct tccgtgattc cgagtgggat ttgcagttcc atattcaggt
     5461 agccctggcg acgcagaact ccgccctggc ggctatcgtt gaaaaaatgt ggacccagcg
     5521 tagtcataac ccgtactgga aaaaactgca cgaacacatt gatgcccgta ccgtcgataa
     5581 ctggtgtgat gaccacgatc aaatcctcaa ggcgctgatt cgcaaagatc ctcatgctgc
     5641 taagctggca atgtggcagc acctggaaaa caccaagatc atgttattta acgaaaccag
     5701 cgacgatttc gagttcaatg ccgaccgcta tctgttcgcc gaaaacccgg tggtgcatct
     5761 cgatacagcc accagcggca gtaaatgaaa ttcctgtccg acaggcgcta cagtgcgcct
     5821 gtttgtctcg tcggtaagcg aaaagtataa agtgtcagcc tgtgtaaatc ctctcgcctc
     5881 cctcccctgc attcagcaaa atcagactcc acggacatgt aattttgata acgaacaacg
     5941 ttgacctttg ttacaattag attcaatttg aatttatgct tttgaatgct ttcttatctc
     6001 acgatttaac agggaatagt tcaggctgtg ttgatgtatc aaacccgcag aacataccaa
     6061 gacagcaata acattgcggt agtgcatcat ttaaaaccag cgtggcgtta accgattcac
     6121 caggaataat gaatggaact tttgacccaa ttgctgcaag ccctgtgggc gcaggatttt
     6181 gaaaccctgg ccaatccgtc gatgattggc atgttgtatt ttgtcttgtt tgtcattttg
     6241 ttccttgaaa acggcttgct tccggcggcc tttttaccgg gcgacagttt actggtactg
     6301 gtcggcgtat tgattgcgaa aggcgcgatg ggctatccgc aaacgattct gctgctgacc
     6361 gttgccgcca gcctcggttg ctgggtcagc tatattcagg ggcgatggct gggcaatacc
     6421 cgcaccgtac aaaactggct atctcattta cccgcgcatt atcatcaacg cgcacaccat
     6481 ctttttcata aacacggttt atcggcgctg ttaattggtc gctttattgc gtttgtcaga
     6541 acactgctgc cgacgattgc cgggttatca gggctgaata acgcgcgctt tcagttcttc
     6601 aactggatga gtggtctgct gtgggtgttg atcctgacaa ctctgggtta catgctcggc
     6661 aaaacgccgg tatttttaaa gtacgaggac cagctgatgt catgcctgat gctgctcccg
     6721 gtggtgctgc tggtgtttgg cctggcaggt tctctggtcg tgttatggaa aaagaaatat
     6781 ggaaatcggg ggtaagggat gcaaatacct cgcatgtcac ttcgccagct agcctggtcc
     6841 ggcgctgttt tacttctggt cagcacacta ttgctggcct ggtccgcggt tcgccagcaa
     6901 gagtctacgc tggcgattcg tgccgttcat caagggacaa cgatgccaga cggtttttca
     6961 atctggcatc accttgacgc tcacggcatt cctttcaaaa gtatcacccc caaaaacgac
     7021 actctgttaa ttacctttga ctccagcgac cagagcgccg ccgcaaaagc ggtcctcgac
     7081 agaacattgc cccatggcta catcattgcg cagcaggaca ataacagtca ggctatgcag
     7141 tggctgaccc ggttgcggga taactctcat cgcttcggat aacttcctgt tatccgaaac
     7201 agatcactca ctttggtgat ttcaccgtaa ctgtctatga ttaatgaggc ggtggttaac
     7261 actaccgctt cgttgtctgg acaatcgttc ctttgtaata ggtccgaatc acaatggaag
     7321 gttcaagaat gaaataccgc atcgctttag ctgtttctct ctttgctctt agtgccggta
     7381 gttatgccac taccctgtgt caggaaaagg agcaaaatat ccttaaggag atcagctatg
     7441 ccgaaaaaca ccaaaaccag aatcgtattg acggtctgaa taaagccctg agtgaagtcc
     7501 gggccaactg ttcagatagc cagctgcgtg ccgatcatca gaagaaaatc gcaaagcaga
     7561 aagatgaggt ggcggaacgc cagcaagatt tagccgaggc gaagcaaaaa ggcgatgcag
     7621 ataagattgc caaacgcgaa cggaaactgg cagaagcgca ggaagagctg aaaaagctga
     7681 aagcgcgcga ctactaactc acaatagtca ctacttactc acctggagaa aactatgtcg
     7741 aaagaacaca ctacggaaca tctgcgtgct gagttgaaat ccctttccga tacgctggaa
     7801 gaggtgctta gctcatctgg cgagaagtca aaagaagagt tgagtaagat tcgtagcaaa
     7861 gcggagcagg cactgaaaca gagccgttat cgcctgggtg aaaccggtga tgccattgcc
     7921 aaacaaaccc gtgtcgcggc ggcgcgtgcc gatgagtatg tgcgtgaaaa tccgtggacg
     7981 ggcgtgggca ttggcgcagc aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa
     8041 ttatggcgga cactcatcac gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc
     8101 gaattgtttc catcatggtt gaaatggtgg agacacgtct gcggctggcg gtggtggagc
     8161 tggaagagga aaaagcgaat ctctttcaac ttttactgat gctgggcctg acgatgcttt
     8221 tcgctgcatt tggtcttatg agcctgatgg tgctagttat ctgggcggtt gatccacaat
     8281 atcgcctgaa tgcgatgatt gccaccaccg tggtgttgct gctactggca ctgattggcg
     8341 gcatctggac gttacgtaaa tcgcgtaagt ctacgttgct gcgccacacg cgccatgagc
     8401 tggcaaacga tcggcagctg ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa
     8461 cgacgtaagg cgcaactgct tagccagatc cagcaacaac ggctggatct ttccgccagt
     8521 cgtcgtgaat ggctggaggc aacaggcgct tacgatcgtc gctggaatat gctgttgagt
     8581 ctgcgctcct gggcgctggt tggcagtagc gtgatggcta tctggacgat tcgccatcct
     8641 aatatgctgg tccgctgggc cagacgcggt tttggtgtat ggagcgcctg gcgtctggtg
     8701 aaaacaaccc tcaagcaaca acagcttcgc ggttaacttc ctctctggcc ggagaagttc
     8761 cggccttatc cnnnnnnnnn nttgctaaca atcacgcttc gtcacgttta tgattctctc
     8821 catcgacagc aacgacgcta atagcgcgcc cttgcacaaa aaaacaatca gcagcctgag
     8881 cggcgcagtg gagagtatga tgaaaaaatt agaagatgtt ggtgtactgg tagcgcgtat
     8941 tttaatgccg attctgttta ttacggcagg ctggggaaaa attactggct acgcgggtac
     9001 ccaagaatat atggaagcaa tgggcgtccc cggctttatg ctgccgctgg tgattctgct
     9061 tgagtttggt ggtggtctgg caatcctgtt cggtttcctg acacgtacca cagccctgtt
     9121 tactgcgggc tttacgctgc tgacggcatt tttatttcac agcaactttg cagaaggcat
     9181 gaactcgctg atgtttatga aaaacctgac tatttctggc ggattcctgc tgctggcaat
     9241 taccggtcca ggcgcattca gcatcgaccg cctgctgaat aaaaagtggt aagcacgcgc
     9301 tatacttaac gataaaaaac gagggggaag ctcctcgttt ttgctattgg aggatagaaa
     9361 aatgggtcaa ctgattgacg gcgtctggca tgacacctgg tacgatacca aatccaccgg
     9421 cggtaaattt caacgttcag cttccgcatt tcgtaactgg ctcactgccg atggcgcgcc
     9481 aggccccact ggcaaaggcg gttttgccgc agagaaagat cgttatcatc tctatgtttc
     9541 actcgcctgc ccgtgggcac accgcacgct gatcatgcgt aagctcaaag gactggaacc
     9601 gtttatttcc gtttccgtag tgaacccgct gatgctggaa aacggctgga cctttgatga
     9661 cagttttccg ggagcaaccg gcgacacgct ctatcaacat gaatttctgt atcagcttta
     9721 tctccacgcc gatccacact acagcggacg agtgactgtt cccgtgctgt gggacaaaaa
     9781 aaaccacacc atcgtcagca acgaatcagc agaaatcata cgcatgttca atacnnnnnn
     9841 nnnngcgttt gatgcgctgg gtgcgaaagc tggagattac tacccaccag ccctgcaacc
     9901 gaaaattgac gaacttaacg gctggattta cgacaccgtt aacaatggcg tgtataaagc
     9961 cggttttgcc accagccagc aagcctacga cgaggcggtg gcgaaagtgt ttgaatcgct
    10021 ggcgcgactg gaacagattt taggtcagca ccgttacctg accggcaacc agctaaccga
    10081 agccgatatt cgcctgtgga ccacgctggt gcgttttgat ccagtgtatg tgacccactt
    10141 caagtgtgat aagcaccgca tcagcgatta cctgaatctg tatggcttcc tgcgcgatat
    10201 ctaccagatg ccgggaattg ccgaaacagt caatttcgat cacatccgta atcattactt
    10261 ccgcagccat aagaccatca accctacggg aattatttca attggtccgt ggcaggatct
    10321 cgatgaaccg catggacgag atgttcgctt cggttaaaaa aaggcacctt acgaggtgcc
    10381 tctgataatt agctaagtac gtgttgttca tgtcggatgc ggcgtaaacg ccttatcaaa
    10441 cctacaaaac atttatattc aatatgttgc aatgaccgcg caggccagat aagcgtagcg
    10501 catctggcaa ttttgcgctt gcccttatcc ttcacagcgc cttattaatt catcgcgata
    10561 atatttactg ccatttcatt cacgtctatt cttaatttgc tgctttaaaa acaagtgatt
    10621 gagcaaaatt gaggcaaaaa tggactggta tctgaaagta ctaaaaaatt atgtcggttt
    10681 ccggggtcgt gcgcggcgca aagagtactg gatgtttatt ctggtcaaca tcatctttac
    10741 gttcgtgctg gggctactgg ataaaatgtt aggctggcaa cgtgccggtg gcgaaggcat
    10801 cctgacgaca atctacggta ttctggtgtt tttaccatgg tgggcagttc agttccgccg
    10861 cctgcacgac accgaccgct cggcgtggtg ggcactgcta ttcttaatcc cgttcatcgg
    10921 ctggcttatt atcatcgtct ttaactgcca ggcgggtacg ccaggcgaaa accgctttgg
    10981 accagatccg aaactggaac aagaataata attaagcacc ttgtaaaagg tgcttaatcg
    11041 attattttcc gctaaaaagt ttgggaattt cccgcagaca ccaggatttc gcctccccca
    11101 tactgtcacg acgccaggcc ataataatat cgatctcgct ggtcgattcc gggctgacga
    11161 cacgcaaccg cccttccgca atatcttttt cgaccatcgg atacggcatc gtcgccacgc
    11221 caagccccgc cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct
    11281 gtttgtccag caactgtacg gtcaacaccg ggcgctcacg agcggtatcc gccaccgcaa
    11341 ttccacgata tttcacgcgc gtcacttcag ataacggttc cggctcctga tgaatcgggt
    11401 gatccggcgc ggcaacatag acgttcatta aggtatagag tttgcgcgag ttgatctccg
    11461 acgaggaacg aaaatgcata tccggc
//
LOCUS       DAWDRC010000045        11424 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2944_length_11424_cov_2.349987, whole genome shotgun sequence.
ACCESSION   DAWDRC010000045 DAWDRC010000000
VERSION     DAWDRC010000045.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11424)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11424)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2944_length_11424_cov_2.349987"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    448..457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1579..1588
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3111..3120
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5993..6002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11198..11207
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2772 a         3075 c         3021 g         2506 t
ORIGIN      
        1 cggcctgaaa acgggcgttg atatcatcaa agcggcgatt ctcggcgcag aaagcttcgg
       61 cttcggcact ggcccaatgg tcgcgctcgg ttgtaaatat ctgcgaattt gccacctgaa
      121 caactgcgcc acgggtgtag caactcagga tgacaaactg cgtaagaatc actatcacgg
      181 cctgccattc aaggtgacga attactttga atttatcgcc cgtgaaaccc gcgagctgat
      241 ggcacagctg ggggtaacgc gtctggtgga tctgattggt cgcaccgacc tgctgaaaga
      301 gctggacggt ttcaccgcca aacagcagaa actggcgctg tcgaagctgc tggaaactgc
      361 cgagccacat gcaggtaagg cactctactg caccgaaaac aacccgccgt ttgataacgg
      421 cctactgaac gcgcagttgc tgcaacannn nnnnnnnggc aaaaccgttt gttgatgaac
      481 gccagagcaa aaccttctgg ttcgatatcc gcaacaccga ccgttctgtc ggcgcatcgc
      541 tttcaggcta tatcgcccag acgcacggcg atcaggggct ggcagccgat ccgatcaaag
      601 cgtacttcaa cggcaccgca ggccagagct tcggcgtgtg gaacgcgggc ggcgtggaac
      661 tgtacctgac cggtgatgcc aacgactatg tcggtaaagg catggcgggt ggcttaatcg
      721 ccattcgtcc tccggttggc tcagccttcc gcagccatga agcaagcatt atcggcaaca
      781 cctgcctgta tggcgcgacc ggaggtcgtc tgtatgccgc aggccgcgcg ggtgaacgtt
      841 tcggcgtgcg taactccggt gctatcaccg tggtagaagg cattggcgac aacggctgtg
      901 aatatatgac gggcggtatt gtctgtattc tgggtaaaac cggcgttaac ttcggcgcgg
      961 gcatgaccgg tggcttcgct tacgttctcg atgaaagcgg cgatttccgc aaacgcgtta
     1021 acccggaact ggtcgaggtc ttaagcgttg acgatttggc gatccatgaa gagcatctgc
     1081 gtggtcttat caccgagcat gtgcagcata ccggttccca gcgcggtgaa gagattctgg
     1141 cgaactggtc aaccttcgcc acgaaattcg cgctggttaa accgaagtcc agtgatgtaa
     1201 aagcactgct gggtcaccgt agtcgtagcg cagcagagtt gcgcgtgcag gcgcagtaag
     1261 gggtagcaac aatgagtcag aatgtttatc aatttatcga cctgcagcgc gttgatccgc
     1321 caaagaaacc gctgaagatc cgcaaaattg agtttgttga aatttacgag ccgttttccg
     1381 aaggccaggc caaagcgcag gctgaccgct gcctgtcgtg cggcaaccca tactgcgagt
     1441 ggaaatgccc ggtacacaac tacatcccaa actggctgaa gctcgccaat gaggggcgta
     1501 tttttgaagc ggcggaactg tcgcaccaga caaacaccct gccggaagtt tgcggacgtg
     1561 tctgcccgca agaccgtcnn nnnnnnnngg cgaaggttcc tgcaccctga acgatgagtt
     1621 cggtgcggtg accatcggca acattgagcg ttatatcaat gataaagcgt tcgagatggg
     1681 ctggcgtccg gatatgtccg gcgtgaaaca gaccggtaaa aaagtagcga ttatcggcgc
     1741 aggtccggcg ggtctggcgt gcgcggatgt cctgacgcgc aacggcgtaa aagcgatcgt
     1801 cttcgaccgt cacccggaaa ttggcggctt gctgaccttc ggtattccgg ctttcaagct
     1861 ggaaaaagag gtaatgacgc gccgccgtga aatcttcacc ggcatgggta ttgaattcaa
     1921 actcaatacc gaagtgggcc gcgacgtgca gctggacgat ctgctgagtg attacgatgc
     1981 cgtgttcctt ggcgtcggga cttatcagtc aatgcgcggc gggctggaaa acgaagacgc
     2041 cgatggcgtg tacgcagcgc tgccgttcct tatcgccaac accaaacagt taatgggctt
     2101 tggtgaaacc cgcgacgaac cgttcgtcag catggaaggc aaacgcgtgg tggtccttgg
     2161 cggtggcgac actgcgatgg actgcgtgcg cacgtctgta cgccagggag caaagcacgt
     2221 tacctgtgcc tatcgtcgtg atgaagagaa catgccgggt tcccgccgcg aagtgaaaaa
     2281 cgcgcgggaa gaaggcgtag agttcaaatt caacgtccag ccgctgggta tagaagtgaa
     2341 tggcaacggc aaagtcagcg gcgtaaaaat ggtgcgcaca gaaatgggcg aaccggatgc
     2401 caaaggccgt cgccgcgcgg agattgtggc aggttccgaa catatcgttc cggcagatgc
     2461 ggtgatcatg gcgtttggtt tccgtccaca caacatggaa tggctggcaa aacacagcgt
     2521 cgagctggat tcgcaagggc gcattatcgc cccggaaggc agcgacaacg ctttccagac
     2581 cagcaacccg aaaatctttg ctggcggcga tatcgtccga ggttccgatc tggtggtgac
     2641 cgctattgcc gaaggtcgta aggcggcaga cggcattatg aactggctgg aagtttaagc
     2701 tctgtcccag cgccgcctcc tcagaaaaga gtcggcgctg aaattctcct ttctttctgg
     2761 aaagcgcctc gcataatctg aagaaaacca atgtaatacg ctcatctgga ttgttaccgg
     2821 tagatgcccg gcgtatagct gattccatta ttcaacgcag cgccatataa agggacagat
     2881 aatggaatcg ctctcagaga gaacctcaac aggctaccag caaatccacg acggtattat
     2941 tcacctggtc gatagcgccc ggacggaaac ggtacgtagc gttaacgcgc tgatgaccgc
     3001 gacatactgg gaaattggcc gacgaattgt cgaatttgaa caaggtggcg aggccagggc
     3061 tgcgtatggt gcgcagctaa tcaagcgact atcaaaggat ttaagtctaa nnnnnnnnnn
     3121 tctaaggtat aagcgtgggt tctctgcaaa aaacttacgc caaatgaggc ttttttacct
     3181 cttttttcaa catgttgaaa ttcgccagac agtgtctggc gaattaacac cattgggaat
     3241 tccgcagaca ccatctgcag aattcccatc ggcaaagatt tggcagacgc tgtctgccaa
     3301 atctttcccc ctgccctggt ccacttacgt ccgtttgctt tctgttaaaa acgctgacgc
     3361 tcgtagcttt tatgaaaaag agacgctccg ctgtggctgg tctgttcgcc agctagagcg
     3421 gcaaattgcg acccaatttt atgagcgaac actactgtca catgacaaat cagccatgct
     3481 gcaacaacac gctcctaccg agacgcatat tcttccgcag caggcgatac gcgatccctt
     3541 tgtgctcgaa tttctggaat tgaaagatga atattcagaa tctgatttcg aggaggcgct
     3601 gatcaaccac ctgatggatt tcatgctgga acttggggat gattttgcct ttgttggtcg
     3661 gcagcgaagg ttacgcattg atgacaactg gtttcgtgtc gatctgctgt ttttccaccg
     3721 ccgtttacgc tgcctgctaa tcgtcgatct aaaagtgggc aaattcagct atagcgatgc
     3781 cggacagatg aatatgtatc tcaactacgc caaagagcac tggacgctac cggatgaaaa
     3841 tccgcccatc ggtctgattc tctgtgcaga gaaaggagcc ggcgaagcgc attatgcact
     3901 ggctggtttg cctaacaccg ttctggcgag cgaatataag atgcaactac ctgatgaaaa
     3961 acgactcgcg gatgaactcg ttcgaacaca ggcggtgcta gaggaaggct atagacgccg
     4021 ttaatttcag cggtttcgaa ttctgcagac agtgtctgca gaatctttaa aacgaactaa
     4081 attttaagcc attaaatcag ccttaatctt cactaccacc tttttaatct caccaggttc
     4141 gccaacaacg catcccggtt tatgcggttc tcctggcata aacacggcga acattcccgg
     4201 tttcaggatg atggcttgct cgttttcaat ggcgctgcaa agctgataat catcttcatg
     4261 gtggaactct tcacactgac gcgcagtgcc tgccatgcca aacagaatcc gttcctcacc
     4321 gtttaataac agctggatat caatgtattg ttcgtgcaat tcggcttttt tctcaacggg
     4381 cgattgagta ttaaatgtca tgacattcat aaagatattg tcgccctgta attcgtaacg
     4441 gccaggcgtt ttttcttgcg gtctggcagc taatgccaac gttaacgcgt cctgtaacgc
     4501 aggatgtaat ccggcagacg gtaatgactg tacttcaccc atcatcataa tatttctccc
     4561 tgggccaaca gcgcagcccc aagtaaacct gcatcgtggc ggtagtgcgc cgccagtaaa
     4621 tcaacatgaa atgccgctgg ctcctgcgcc agatacgttt ccaccagcgc cagataccct
     4681 tctgccagac caacgctgcc accgaccacc acgcactggc aatcagttgt ggctttaata
     4741 tcagcgatca gccttgcaag cgtacgtgcg gagcggtgaa tcagttgctg cgcctgctca
     4801 tcaccctgcc cggcgcgcgt gaaaatagtt ttcgcattcg caccagccaa ctccccctgc
     4861 gctgccgttg caatgccgcg accagaagca atcgcttcca cgcaacctgt gcgtccacag
     4921 ccgcagactg gaccatgcgg atcggcaagc gtatgcccga tatgccccgc cagaccgcca
     4981 gggccggtac gcagtttacc gccgctcact acaccgccgc caacgccggt ggaaacagtg
     5041 ataaacacca tatcggttat atcgccatcc agcgcctgat actccgccca tgctgcggcc
     5101 tgcgcatcat taatggcaat ggtcggcaaa ttggtaagtt gctccagcgt tttgactaac
     5161 ggaaagtgta acaatccacc gagattatgc ggattcagcg ccagcaagct gccgtcgcga
     5221 ataattccgg ttgaagcgat agcaacccgc tgcgcatgtg cttgcaacgg agagactaat
     5281 gcggataagg catcacgcag cgcctgcgga gtctggctgg caggcgtagg aagttctcgg
     5341 cgatcgcgga tctgcccgtc agcgccaatc agcgcggcgg caagtttagt accgccgata
     5401 tcaatcgcca gtgtggtcat agcaccgcct ttttcatcgc tgtgttgtac cactgacaaa
     5461 tgtgttcaag acgcgtaatg gcagaaccga ccgtcaccgc ccacgcgccg tggcgcatcg
     5521 catccgccgc ctgagcaggc gtgttgtaac gcccttcggc aatcacccgg catccggcgt
     5581 cactcaacgt tttcaccagc gccagatccg gctcttctgg cgtttcaggc gtggtatagc
     5641 cagaaagcgt agtgccaata atttcggctc ccagcttttg gcatgccagg ccgtcttccg
     5701 gcgttgagca gtcggtcatc gccagtaaac cgtgatggtg aatacgtgcc agcagcgttt
     5761 caactggcac cggacgcggt cggtcggtgc cgtcaatcgc gataatgtcc gcgcccgcct
     5821 gcgccagcgc atcaacatct tcaatatagg ccgtgatgcg taccggagaa tcctccagat
     5881 cgcgcttcac aatcccaata atcggcacgc tcaccaccgc acgcgtggct tgcagatttg
     5941 ccacaccttc aatacgaatg gcaaccgcgc ccgcctgctc tgccgctaat gannnnnnnn
     6001 nntaatgcca tggcggcaac gatttcgggt ttatcgagcg ggctgtccgg aaccggctgg
     6061 caggagacaa tcaggccacc gttagcagcg attttttgat ccagttgtgc aagtaacgac
     6121 atacatcttc ccttagcgaa aggcccggta cagagaccgg gcaacaggat taacttttgg
     6181 ttttgactaa atcgtttttg gcgctgccaa acggcacggc accgctaaat ggtttgccgt
     6241 cgatagcgtc atgagtacgc aatgcttccg ggcgcagcca acgctgaaca cgagaaggca
     6301 tatccagacc aatcagcagg atcaccacaa atgtcagact gaacgagagc gatgccagcg
     6361 cagtacccag atccagacgt tgagcgatca acgcgccgag gattggggcc agtgcaccgc
     6421 ccaatgcgcc aacgttgtag gtaaagccca ggcccgctgc acgctggtcg gtatcgaaat
     6481 aaccgccaat cagtttcggt aagatcccgg cgatcccttg tccaagcatt tgctggaaga
     6541 acagtaacag accgagcacc cagacgtttg cgccgccaat cgcaaatacc ggaataatca
     6601 gcagctgcga ggccagcaga ctacaaacgt acgctttgcg ggttcccagc cagtcaccga
     6661 ggaagccacc gacgcagcac cccaccgccg cgccaaagcc actaaagaac agcacattgg
     6721 cgacagtatg cgggtcataa gccagatcgg ttttcagata cgttggcagc agcgcctgaa
     6781 tcggccatga gtagaggaaa gcgaacaaca cgaccaccat cagcattacg cccgttggcc
     6841 agcgtttgcc cgtactctgc accataaagc tgataaagat tgcggcgcat aacagcccaa
     6901 gaacagcgac gatcgcggca ttttgcaggt taccggcgaa gcagaaccac agcgcagtag
     6961 ccgccgccag tgtcattacg atattggcaa tgcgatgttc accacggtag agaatgtcta
     7021 ccatcgtgcg tactggtgct ttacccccgt gtttctcttt ccagtcttcc gcttccggga
     7081 tgtttttacg cagccagaga gcaaagatga ttggcaaaat gccgataaag aacagcgcac
     7141 gccagcccca gaccggaacc accagactat agacctgagc ggcaacgacg gcccccactg
     7201 agaagcctga aatcagaaaa ccactggctt tgttacgcag atgctttggc cagctttcaa
     7261 tgacataggt ggcgctggaa ccgtattcac ccgccatccc catgccgatg accagacgag
     7321 cgataaacat ggtgatgtag cctggcgcaa agccgcaggc cagcgtcccg gccgagaaga
     7381 gaacgatgct ggtgaccatt gccagacgac gcccgtagcg gtcacccata gcgccgagca
     7441 tcaggccgcc gaaccagcga gagataaagg ctgcggagat cagacttgcc gcctgcaccg
     7501 tcgtcagccc gaattcaccc tgtacttcgg tgagtaccag ggcgattaaa acgaaatcaa
     7561 aaccgtcaag cagatatccc aaccaggcag cggaaaatgc ccgccattgt gcgcggttaa
     7621 gatggcgata ccacgggata ttctgggttg tagtactcat tgtgagtctc ccgcggtggg
     7681 cgatgcccac acgctttggt atgaaaattg tagggtacag atgcgtttat ttcccctcac
     7741 ccggtagggg cgagcgaggg gaaacaactc acccgcgctc ttgcatcaac tgctgggcca
     7801 gcgccttcag ttctggcaga tatttttcat ctaccggacc aaacggtttg cggcacagcg
     7861 gcacagaaac gacatccata taatgcagga cagttttcag gccgcggaat acgccagttt
     7921 tgatcagtaa atcaatgact ttgttgcact cagtttgcag tttctgcgcg gtctggatat
     7981 cgccttcttt cagcgccttc acgatcccct gataacgcca gcccatgatg ttgtaagtgc
     8041 tgccgatacc gccgtctgcg cccgccagaa gaccagaggc gaagatttcg tcgtaaccgt
     8101 tatagagcac cagatcagga tgttcacgac ggatctgctc catctgatag agatcgccag
     8161 aggtctgttt cagcgcacct acaccaggca gcgtaaccag ggtgttgatc tgatccaggg
     8221 tcagttttac cccgctcagg gctggaatgt tgtacaccac catcggcaac ccatccgccg
     8281 aatcaataat tgcccgatag tgatcgcagt gttcttcaaa gctgaaagga tagtagaacg
     8341 gcgtgacggc ggagacggca tcgaagccat aacgtttagc cgatgccgca agttgttggc
     8401 tttcggcggt gctgacgcaa ccgacgtggg cgatgagttt aatcttacct ttcgcctctt
     8461 cagcgacgat ttccagtacc tgttcacgct ctgaaaggct ctgtacaaag gcttcgcctg
     8521 tcgaaccacc cacgtacaaa ccgtcgatgc cctgctgaat attgaactga accaggcggc
     8581 gcagactcgc tttatccagt gcttgttgtt ggtcaaaagg agtcaggagt gcagccatta
     8641 cgccacgtaa attcgttgcc ataaatacct ctgaagtgat gcttgtctga taaacgatat
     8701 acctttatac ctgttatacc agatcaatta agcaacaccc catacagaaa gcttataatg
     8761 cgatctgctt cactaaagtg gcattatttc tttttgttgg tggtctgacc gaaggcgtgc
     8821 caggtagcag agacgctgtt gagatgcgat tgcaacgcgc gatcggcttc gtcaggatca
     8881 tgacggcgga tcgcgtcaac gatcgcaata tgctgttgat agctaacgtt gttatgctca
     8941 tgcagagcct gatcggccac ggttggacgt gcggcgatta gccagtcgag cagggcaacg
     9001 tggatcgcca tgaagattgg gttaccaggg atctccgcca gaacgcggtg gaaatcaacg
     9061 tctgagcgaa tgaatgccgc gttgttatcc agcgactgac tgttgatttc cagtgctttt
     9121 gccagcaaat cgatttgctc atcggtggcg tgttcagccg catagcgcac caggctggat
     9181 tcaaagaaca gacgtaattg ttcgaaatgg gcaatcctac caggatgaga aaggaaatct
     9241 ttcgccatgc cggaaagctc gccgatgatg gtgtccgcag aaggacgtgg gacgcgggca
     9301 cgttcgccgt tgtttatttg caccagacct ttgcgtttta acgctgccag cgcttcgcgc
     9361 accgaaggac gcccgacgtt aaagaacgcc atcagttcgc gttcagacgg taattgttca
     9421 ccttcgccaa attcacgacg gcggatcatc tgttccagct cttcttccac catttcggag
     9481 agttttttac gcgccagcgg gcggctgcgc aagttgcgcc cgatagtggt agtagaatct
     9541 tcagcttgcg gatcaaatgc gttcatagga ctcattcagg aaaatgcgtt agcggataat
     9601 aacgacgcta tcgtagcaca gtatcaaagt caggcgaaaa gatgacagat agcaaaacag
     9661 gccacatggg cctgttttca ttttacttca caacgcgtaa tgccggtcga ccaccgcgtg
     9721 gtggctgcgg aggttcatcg tcaggatgag tttcatcatc gtgatctggc ttgtcgccat
     9781 cgataaccga cataacggtt tcgttgtctg ccgatgcctc ttcatcattc atgatgctgg
     9841 catcttcatc gtaggcagct tcaggctcaa acatcgtgcc tgcgccattt tcacgggcgt
     9901 agatagccag cacggcagcc agcggcacag aaacctgacg cggaatgcca ccaaagcgcg
     9961 cgttaaagcg cacctcatca ttcgccagtt ccagattgcc gacagcacgc ggcgcaatgt
    10021 tgagtacgat ttgcccgtca cgcgcatatt ccataggaac ctgcacgcct gggagcgtca
    10081 catccaccac caggtgcggc gtgagctggt tatccagcaa ccactcatag aatgcacgca
    10141 gcagataggg acgacgtggt gttagctgtg acaaatccat acagattaac tccggcccag
    10201 acgcatttca cgttctgctt cagttaaaga agcaaggaaa gagtcacgtt caaagacgcg
    10261 ggtcatatag cctttcagct ctttcgcacc cgggccgctg aactcgatgc ccagttgcgg
    10321 cagacgccac agcagaggag caaggtagca atcgaccagg ctgaactcat cgctcaggaa
    10381 gtacggcttc tgaccgaaga ccggcgcaat cgccagcagt tcttcgcgca gttgcttacg
    10441 tgcggcatcg gcttcagaag ctgaaccgtt gatgatggtg ttcatcagcg tgtaccagtc
    10501 tttttcgatg cgatgcatgt acagacggct ttcaccgcga gctaccgggt aaacaggcat
    10561 cagtggcgga tgcgggaaac gctcatccag atattccata atgatgcgag attcccacag
    10621 ggtcagctca cgatccacca gggtcggaac gctctgattc gggttgaggt caatcagatc
    10681 ctgaggcgga ttgtcctttt ccacgtgttc gatctcgaaa cttacacctt tctcagccag
    10741 cacaatgcgg acctgatggc tatagatgtc agtaggaccg gaaaacagcg tcattaccga
    10801 acgtttgttg gcagcgacag ccatgaaaac ctccaggtat agtcagaatt tttactgcta
    10861 ccagccacca ggtggccagt cagaagttgt gttacccagt aaggaacgac tctctttgtt
    10921 cgaaaatcaa acaaaaaatg agcaataccc gacatttggg cagaaaattg gatgatagtt
    10981 taccagattt tgcgaccttt gtggtgagtc gatgccggaa atggggaaaa agagatgcgc
    11041 tttagtctga aatagttgac ttagtccctt attggcgatg tggtttttgt tttacctgtc
    11101 tgtcaggtgg cagcaaaaag caactttcca ttttttacgc tgatgcaggt tttagccata
    11161 aaaaaacccg ccgaagcggg ttttttcgca aattgttnnn nnnnnnnatt aacgtttgga
    11221 gaactgcgga cgacgacgtg ctttacgcag accgactttc ttacgttcaa cctgacgagc
    11281 gtcacgagta acgaagccag ctttacgcag ttcagaacgc agggactcgt cgtattccat
    11341 cagagcgcgg gtgataccgt gacggatcgc accagcctga ccagagatac caccaccttt
    11401 aacggtgatg tacaggtcca gttt
//
LOCUS       DAWDRC010000046        11374 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2957_length_11374_cov_2.394911, whole genome shotgun sequence.
ACCESSION   DAWDRC010000046 DAWDRC010000000
VERSION     DAWDRC010000046.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11374)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2957_length_11374_cov_2.394911"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1750..1759
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3627..3636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4827..4836
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7776..7785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2589 a         3167 c         2827 g         2751 t
ORIGIN      
        1 taagttctct ccccttttgt cgattgcccg atgtgacgct aaagcgtctt atcgggcctg
       61 tacgttgcac gagagtctga cgttagcgca gaattatctc agcaaatcca atctcttcgt
      121 ttccccacga taatatgacc aacctctcat aatttaaatt taccccgctc tggtgattct
      181 caagcacgta atgttacccg tatcattccc atgggcacca aacacactca taacatctga
      241 ctacactaac tcgttttagg ggtcatcagc cgtggtgccg taacatctct gttcccagtt
      301 gggacaacca ggattttccg tcagaaacct gacgttaccc ataacaaatt aaaggccagg
      361 taaatcatgc cattagtcat tgttgctatc ggtgtaatct tgttattact cctgatgatc
      421 cgcttcaaaa tgaacggctt catcgctctc gtcctcgtgg cgcttgctgt tggattaatg
      481 caaggaatgc cgctggataa agttattggc tccatcaaag ctggtgtcgg cggaacgctc
      541 ggtagccttg ccctgatcat gggttttggt gcgatgctgg gcaaaatgct ggcagactgc
      601 ggtggtgcac aacgtatcgc taccacactg attgccaaat ttggtaaaaa acacatccag
      661 tgggcggtgg tactgaccgg ttttaccgtt ggttttgccc tgttctatga agtgggtttc
      721 gtgctgatgc tgccgctggt gtttaccatc gcggcttccg cgaatattcc actgctgtat
      781 gttggtgtac caatggcggc tgcactgtct gtgacccacg gcttcctgcc accgcatccg
      841 ggtccgactg caattgccac cattttcaat gccgatatgg gtaaaactct gctgtacggt
      901 actattctgg caatcccgac cgtgattctc gccggtccgg tttacgctcg cgtgctgaaa
      961 ggtatcgata agccgatccc ggaaggtctg tacagcgcga aaaccttcag cgaagaagag
     1021 atgccgagct ttggcgtcag cgtctggacc tctctggtgc cggtggttct gatggcgatg
     1081 cgtgcgattg ccgaaatgat cctgccgaaa ggtcacgctt tcctgccggt ggcggagttc
     1141 ctcggtgacc cggtaatggc cacgctgatt gccgtgctga ttgcgatgtt cacctttggt
     1201 ctgaaccgtg gtcgttcaat ggatcagatt aacgacacgc tggtttcttc catcaaaatc
     1261 attgcgatga tgctgttgat tatcggtggt ggcggtgcgt tcaagcaggt gctggtagac
     1321 agcggcgtgg acaaatacat tgcttccatg atgcacgaaa ccaacatttc tccgctgctg
     1381 atggcctggt cgattgctgc cgtactgcgt atcgcgctgg gttccgcaac cgtcgcggca
     1441 atcactgcgg gtggtatcgc ggcaccgctg attgcaacga cgggtgttag cccggagctg
     1501 atggttattg cggttggttc cggtagtgtg attttctctc acgtgaacga tccgggcttc
     1561 tggctgttca aagagtactt taacctgact atcggcgaga ccatcaaatc ctggtcgatg
     1621 ctggaaacca ttatctcggt gtgcggtctg gtaggctgtc tgctgctgaa tatggtgatt
     1681 tgataatact gatggccgga tgcggcgtga acgccttatc cggcctacga ttcggcatta
     1741 attagtaggn nnnnnnnnnc cggataagac gcgtcaagcg tcgcatccgg catcaaccgc
     1801 actctacgtc ttcttcgctg ccgctctgcg ccgtctgtcc aaatccttca gcaacttgtt
     1861 cacaccatca tcggcaaaca tcgactcaag cgttgcggaa agtttacgtc gccagttttt
     1921 atactggtaa ctggtgccgg gaatatttac cggatctgcc atatccagcc agtcttccgg
     1981 ctgtagccct aacagggcgc tgttgctatc ggcgatgtag cgctgtaagc cacgattcag
     2041 cgtcggtgtc atcgacatca acgatgcctt atgcccggca cgttttggca ggcaaccata
     2101 tttatgcaga gcatccagca gcccttgctt cgccagttcg cgatcctgat acagaccgcg
     2161 cagtaccact tcatccggat acagccccag cgttttgccc agcgttaaat ccccactctc
     2221 ccagtagccg cgtagcgtag gcaggtcgtg tgtcgccgca accgccatcg actgctctgg
     2281 gtacgctttc ggtgcgcgga atgtcttctc gtggtcgttt tcgaaataga gcactttgta
     2341 ggagtacaca ccgctgctgc gcagcttacc gacaatctct accggtacgg taccgagatc
     2401 ttcaccaatc accatacagc gatgacgttt actttcgagt gccagaatcg agagcagatc
     2461 atccaccgga tagtgaacat acgcgccctg atctgccgtc tcgccatacg gtatccacca
     2521 caaacgcagc atcgacatca cgtgatcaat tcgtaatgcg ccgcagtttt gcatattggc
     2581 acgcaacagc tcgataaacg gttcataggc acgcgcggtg atgatatgcg ggtccattgg
     2641 cggcaatccc cagttctgcc ccaacgggcc gaggatatcc ggcggcgcgc caaccgacgc
     2701 tttcaggcaa tacagttcac ggtcgcacca ggtttctgcc ccaccttctg ctacaccaac
     2761 ggccagatca cgatacaagc caatcggcat ttcatagccc tggcttatct cccagcaggc
     2821 ggcaaactgg ctgtaagcca gccactgcaa ccagagataa aaatcgacgt catcacgatg
     2881 ttcttcgcag aactggcgca cttctggtga atccacgttc tgatacatct ctggccatgc
     2941 tggccagccc cagcgcattt cgtcctcttt cacttgctgg gcatgtagcg catcaaaggc
     3001 tgcctgccag aacaggctgt cgccctgctc tgcaacaaac tggcgaaacg cggtcatctg
     3061 ctcatcatca cgttgcgcga aacctttcca cgccattcgt aatgctgtca tttttagggc
     3121 ggtaaccgtg gagtaatcaa cccagtcggc atcccgcgcc tgttgcagcg tctgttgcgt
     3181 ggtcggcaac tgccaccagg cctgagcctc ttcgctaaga tggaaatctt caactgcgtt
     3241 aacgtcgata taaatcacat tcagccaacg gcgagaagac gggctatacg ggctggcact
     3301 ttccggattt gccggataga gcgcatgaat cgggttcaga ccgatgaacg acccgccacg
     3361 ttttgccaca tccaccagca tcgctttgag atcgccaaaa tccccaatac cccagttttt
     3421 ttccgatcgc agcgtataaa gctgaacgca ggcaccccac aacttttgtt tattcagcaa
     3481 cgcctgcggt tcgtaacagc gtttcggggc gacaatcacc cggcaatgcg cgcgctggtc
     3541 gtcctgggtg agtgtcagcg tgtgataacc ttccggcagc ttcgtcggta gattgaacgc
     3601 tttgcccccc gttacatggc ctttgtnnnn nnnnnncctt cttcggtggt cagcagccag
     3661 ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt ataaaccatg
     3721 acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat cgcgtcaagc
     3781 aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt gatgtaattg
     3841 gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat cgagcttcct
     3901 tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc cgagctaaac
     3961 ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg gtcgcggtac
     4021 agcacatcca cctgcttttg tgcctctaca taagcggcga aatctgccat caccagatac
     4081 ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg cttatcgccg
     4141 tcgctgtatt tgccgctttc cagctctttc aatactgcgt ccagcacctt atctttcttc
     4201 cgccatttca ccgggtcgta gcctttggcc agaattgcct tcacttgttc cacggtatga
     4261 ccaaaaataa agatattttc ttcaccaact ttctcggcga tttcaacgtt cgcaccatcc
     4321 agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt accggaagct
     4381 tctttacctg ccgtcgaaat ttgttcggag atatccgccg ccgggatcag tttttccgcc
     4441 gccgaaacgc aataatccgg caggaacacc accttcaatt tgtcgccaac ctgcggatcg
     4501 ttgttgatca cgtcagccac tttgttgatc gcaaagataa tgtttttcgc caggtagtag
     4561 cccggtgccg ctttcgcgcc aaagaggaag acgcgcggta cgcgatcagc ctgcgggttt
     4621 tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcagatgctg gcgtttgtac
     4681 tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc aataccggta
     4741 cgaactttca caaactctgc cagacggact ttattcgcct gcttgatctc gcgatattgc
     4801 tgacggaatt tcgcatcatc agcgaannnn nnnnnnaatt tttccagatt gatcagttga
     4861 tcgagatcgt tagcccactc ttttttcagt gatttatcca acagagccgc cagtgccggg
     4921 ttgcactgtt tgatccagcg acgtggggta ataccgttgg tgacattatg gaatttgttc
     4981 ggccatagct ggtgatattc cgggaacaga tctttcacca ccagatccga gtgcagcgcc
     5041 gcaacaccgt tcaccgcgaa accgccaacc acacacaggt tcgccatatg cacttgtttg
     5101 tcgtgcacca ccgccagttt ggcccacact ttttcatcgc ccggccaggt tttctccacc
     5161 agcgttttaa agcgagtatt aatttcgtta ataatctgca tgtggcgcgg cagtaagcct
     5221 ttcaccagtt tcacatccca gcgttccagc gcttctggca tcagggtatg gttggtgtaa
     5281 gcgaaagttt tgctggtgat ggcccaggcg tcatcccagc tcatctggtg ctcatcgatc
     5341 agcacgcgca gcagttctgg aatcgcgata gttgggtggg tatcgttcag ctgaataact
     5401 tcataatccg ccagttcgtg cagtttacgc cccgccagat gatggcgacg caaaatatcc
     5461 gctaccgagc aggcacactg gaagtattgc tgcatcaggc gcagcttttt accggcagta
     5521 tggttgtcgt ttggatagag aactttggtc agtttttccg cgttgatgcc ctgctgttcg
     5581 gcacgcagga aatcaccatc gttaaattta gtcagatcaa acggatgcgc atgcgtcgcc
     5641 tgccacagac gcagtggctg cgccacgcca ttacgatagc cgacaacggg gagatcccac
     5701 gcttgaccgg taatggtaaa ctccggctcc cagcgtccat ctttcgtcac tttaccgcca
     5761 atccctacct gcacatccag tgcttcgttg tggcggaacc acgggtagtt accgcgatgc
     5821 cagtcatccg gcgcttcaac ctgtttgcca tcgacaaaag actggcggaa caaaccatat
     5881 tgatagttca gaccgtaacc cgtcgcagac tgaccgacag ttgccattga gtcgagaaag
     5941 cacgccgcca gacgtcccag accaccgttc cccagcgccg ggtcgatctc ttcttccaac
     6001 aggtcagtca ggttgatgtc ataagccttc aacgaatcct gtacatcctg ataccagccg
     6061 agattcaaca ggttgttgcc cgtcaggcga ccaattaaaa actccattga gatgtagtta
     6121 acatgtcgct gattcgccac tggcttggcg aatggctgag cacgcagcat ttcggccagt
     6181 gcttcgctca ctgccagcca ccactggcga ggagtcattt cagccgcaga atttaagcca
     6241 taacgctgcc actgacgtga aagcgcttcc tgaaattgct tatcgttaaa aataggttgt
     6301 gacataggag ttccactttt cttagatttt caacacaacg ttatcgctag tttgccaggc
     6361 tcgatgttga ccttcctcat cctgcggggg attaggcagg gaggagttgc ggggatgagc
     6421 aaggaaatgt gatctcgacc acttaaagct agtgcaatcc acaggattag cagcaaatca
     6481 atgccatacc gcgcagaaaa tctgtatcta agtgcaaaaa atggccgttg cgtattttca
     6541 aaaagcggaa ggtaactcta taaattaagt aaaggagtga aacagtttca caagtaaaat
     6601 atcaaatgtg ctccatctca ttcttaatag atttattaag atcatctttt tagatggcac
     6661 tttcatcaga aatgaagaag aaacccttac ttaaatgaaa ctgatgaaca taaggaaaat
     6721 accgtacaac ccagtattca cgctggatca gcgtcgtttt aggtgagttg ttaataaaca
     6781 tttggaattg tgacacagtg caaattcaga cacataaaaa aacgtcatcg cttgcattag
     6841 aaaggtttct ggccgacctt ataaccatta attacgaagc gcaaaaaaaa taatatttcc
     6901 tcattttcca cagtgaagtg attaactatg ctgattccgt caaaattaag tcgtccggtt
     6961 cgactcgacc ataccgtggt tcgtgagcgc ctgctggcta aactttccgg cgcgaacaac
     7021 ttccggctgg cgctgatcac aagtcctgcg ggctacggaa agaccacgct catttcccag
     7081 tgggcggcag gcaaaaacga tatcggctgg tactcgctgg atgaaggtga taaccagcaa
     7141 gagcgtttcg ccagctatct cattgccgcc gtgcaacagg caaccaacgg tcactgcgcg
     7201 atatgtgaga cgatggcgca aaaacggcaa tatgccagcc tgacgtcact cttcgcccag
     7261 cttttcattg agctggcgga atggcatagc ccactttatc tggtcatcga tgactatcat
     7321 ctgatcacta atccagtgat ccacgagtca atgcgcttct ttattcgcca tcaaccagaa
     7381 aatctcaccc ttgtggtgtt gtcacgcaac cttccgcaac tgggcattgc caatctgcgt
     7441 gttcgtgacc agctgctgga aattggcagt cagcaactgg catttaccca tcaggaagcg
     7501 aaacagtttt ttgattgccg tctgtcatcg ccgattgaag ctgcagaaag cagtcggatt
     7561 tgtgatgatg tttccggttg ggcgacggca ctgcagctaa tcgccctctc cgcccggcag
     7621 aatactcact cagcccataa gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt
     7681 tcggattatc tggtcgatga ggttttggat aacgtcgatc tcgcaacgcg ccattttctg
     7741 ttgaaaagcg ccattttgcg ctcaatgaac gatgcnnnnn nnnnngatgc actcatcacc
     7801 cgtgtgaccg gcgaagaaaa cgggcaaatg cgcctcgaag agattgagcg tcaggggctg
     7861 tttttacagc ggatggatga taccggcgag tggttttgct atcacccact gtttggtaac
     7921 ttcctgcgcc agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc
     7981 gccgcagaaa gctggatggc ccagggattt cccagcgaag cgattcatca tgcgctggcg
     8041 gcaggcgatg cgctgatgct gcgcgatatt ctgcttaatc acgcctggag tttgttcaac
     8101 catagcgaac tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagtttgctg
     8161 gaaaatccgc agttggtatt attgcaggcg tggctgatgc aaagccagca tcgctacggc
     8221 gaagttaaca ccctgctggc ccgcgctgaa catgaaatca aggacatcag agaaggcacc
     8281 atgcacgcag aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg tagtccggat
     8341 gaagcggaac ggctggcaaa actggcactg gaagagctgc cgccaggctg gttctatagc
     8401 cgcattgtgg caacctcggt gctgggtgaa gtgttgcact gcaaaggcga attgacccgc
     8461 tcactggcgt taatgcagca aaccgaacag atggcacgcc agcacgatgt ctggcactac
     8521 gctttgtgga gtttaatcca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc
     8581 gcgtgggaaa cgcaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagctg
     8641 ccaatgcatg agtttctggt gcgcattcgt gcgcagctgt tgtgggcctg ggcacggctg
     8701 gatgaagccg aagcgtcggc gcgcagcggg attgaagtct tgtcgtctta tcagccacag
     8761 caacagcttc agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat
     8821 aacgcccgta gccagctgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc
     8881 gactggatct ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa
     8941 gccgccgctg ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc
     9001 ctgcaaggtc aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaatct
     9061 gcggaaattg tcctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat
     9121 ctcaaccgta acctgttgct gcttaatcaa ctgtactggc aggccggacg taaaagtgac
     9181 gcccagcgtg tgttgctgga cgcattaaaa ctggcgaatc gtaccggatt tatcagtcat
     9241 tttgtcatcg aaggcgaagc gatggcgcaa caactgcgcc agcttattca actcaatacg
     9301 ctgccggagc tggaacagca tcgcgcacag cgtattctgc gagaaatcaa tcaacatcat
     9361 cggcataaat tcgcccattt cgatgagaat ttcgttgaac gtctgctaaa tcatccggaa
     9421 gtaccagagc tgatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg
     9481 atttactccg gttacagcaa tgagcaaatt gcaggcgagc tggaagtggc ggcaaccacc
     9541 atcaaaacgc atatccgcaa tctgtaccaa aaactcggtg tcgcccaccg tcaggccgcg
     9601 gtacaacacg cgcagaaact gctgaagatg atgggatatg gggtgtgagt ttagccggat
     9661 aatgcgtcgg atccggctta catctttaca tcattcaatg ctcacccgcg ttacgccatc
     9721 tgtttctata aaaccaaacc gcaccggcaa gaaacgctcc accacggcga tattggtcag
     9781 cagatggcat gagggatggg cgaccgtaaa ttcccccgtg cccgccagcg ccatcggtag
     9841 caccagttgg tcggcgagat attcccccac cgccgccggg cttgccaggt agcgtttcac
     9901 ctctttcacc aactgtgccg cgaccacctc ggcactgacg cgcttttcac cgacgacaaa
     9961 aaagcgttcg gtgatatttt cactttcgac ttcaagcgag acggtattac ccggcccctg
    10021 gtcgcgcggc aggttatgaa tattctgttc atgcaaggaa aaactacccg ccagtgtagc
    10081 gatttcacgc tcagcaacat ggcgcggcac accagccagt agcacctctc cacgcatctg
    10141 cacaatgttc ccgcgctcgc caagttgcaa gctattaaac gatgccaccg gtgagacttc
    10201 cgttgccacc acgccgcctc cggcaggata aaaaccgtga cgtaacagcg tggtttgctg
    10261 atgaactcct attttcgcca gcagcggctc cagcacccgg cggataaaat ccgcaggcgg
    10321 ggccgacggg ttatcggtgc cgccgctcac ttcaacacgc gaaggtccat cggcaaacca
    10381 cagcgcgggc agcaccgttt gcagcaccag cgtacaactt ccggcgctac cgatagcaaa
    10441 gcggtaatcg ccgccgcgca cggtgccagg tcggaagacc agacgctgcg accccagctc
    10501 agcgccttcc accgttgccc tacaaatttc cgcagccgct tttaccgcgg tcagatgttg
    10561 gcgcaacagc cccggtttcg cccgcccggc acgaatgccg gtgatggtaa atggctggcc
    10621 ggttatcatc gacaggctca gcgccgagcg caggatctgc ccgccgcctt cgccctgtgc
    10681 gccatccagc gcaatcatcc ttttcatcat ttatcctttt acgcacacca cctgacgcag
    10741 ggtatagata acttccacca gatcgctttg tgccgccatc accgcatcaa tatctttata
    10801 cgccatcggg atttcgtcga tcacctcggc atctttacgg cactccacat gcgcggtggc
    10861 acggatttga tcttccacac tgaacagttt tttcgcttta gtacggctca ttacccgccc
    10921 ggcaccgtgg ctgcacgaac agaacgactc ttcatttccc agcccacgga cgataaagct
    10981 tttcgccccc atcgaaccgg gaataattcc atattgacca gcacgcgcag acaccgcgcc
    11041 tttacgcgtc acatagatct cttcgccaaa gtgctgttct ttttgcacat agttgtggtg
    11101 acagttgatc tcttccatcg ccagcgtttg tggctgtttt accgttttct gcgtaacgct
    11161 ctgcaacgcc gttaccacgt tttccatcat cgcatcgcgg ttaaggctgg caaaaagctg
    11221 cgcccaggcc acggctttca ggtaatcatc aaagtattcc gtaccttcca taaagtacgc
    11281 caggtcacgc gatggcaacg tttcaagcgt ttcctgcatc tctttttgcg ccagatcgat
    11341 aaagtaagtt ccgatggcgt taccaattcc gcgt
//
LOCUS       DAWDRC010000047        11277 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2983_length_11277_cov_2.540545, whole genome shotgun sequence.
ACCESSION   DAWDRC010000047 DAWDRC010000000
VERSION     DAWDRC010000047.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2983_length_11277_cov_2.540545"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    580..589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4018..4027
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2749 a         2785 c         2783 g         2940 t
ORIGIN      
        1 ttgggatatg atcgtttttt gttgatcttc ttcacagatt atagccattt catggataga
       61 ataaccctac cttcaactga cacagaaaaa ggtaaaggta aatggaaaat aataaccgct
      121 taatgcctca tataaggcgg acaacccata tcatgaagtt tgcccatcgt aatagttttg
      181 actttcattt ctttaatgcc cgctagtctt ctgactaaag ggcaccccag cgtacaggtc
      241 tccctgactt taagcattac aggttaatac ctgtatccct cggtgctcat atactgctaa
      301 cccttttaaa ctctaaataa ttcgagtcgc agcacatgca acttgaagta tgacgagtat
      361 agccagttac cgggctggtc tgggttattg catctgcaaa aagcaaacta ctgatttatt
      421 tatcagcggt ggggttttgc ttttttccgg cgtgatcaat ttcttctttg agaaattgag
      481 gacctgctat tacctgaaat aaagagatga acaaaatgtc agaattaaaa attgccgtta
      541 gccgttcttg cccggattgt ttttccactc atcgtgaatn nnnnnnnnnt gaatattgac
      601 aaaagtaatt atattgacgt tgccgccatt attttatcag tcaatgatgt tgaacgtgga
      661 aaactcgatg aaatagacgc tactggctat ggcattcctg tttttattgc aacggaaaat
      721 gaagaacgtg taccagcaga atatctgcca cgtatttctg gtgtcttcga gcattgcgaa
      781 tcacggaaag agttttatgg acgtcagtta gaaaccgctg ccagccatta tgaaacgcaa
      841 ctgcgcccgc ccttcttccg cgcactggtc gattatgtca atcagggcaa tagcgctttt
      901 gactgcccgg gtcatcaggg aggtgagttt ttccgtcgcc atccggcagg caatcagttc
      961 gtggaatatt ttggcgaaat gctcttccgc tccgacttgt gtaatgccga cgttgcaatg
     1021 ggcgatctac tgattcatga aggtgcgcca tgcattgcac agcaacatgc ggctaaagtg
     1081 tttaatgccg ataaaacgta ctttgtttta aacggcacct cctcttccaa caaagtggtt
     1141 ttaaacgccc tgctgacacc gggtgatctg gtgctgtttg accgcaataa ccataagtct
     1201 aaccaccacg gggccttgct acaggctggt gcaacaccgg tttatctgga aacagcacgt
     1261 aacccgtatg gttttatcgg tggcattgat gcgcactgct tcgaagaaag ttatctgcgt
     1321 gaactgatta cagaagttgc accgcaacgg gcaaaagagg cgcgtccgtt ccgcctcgct
     1381 gtgattcagt taggcaccta cgacggtact atttataacg cccgccaggt ggtggataaa
     1441 atcggtcatc tgtgtgacta catcctgttt gactcagcat gggtcggcta tgaacagttt
     1501 attccgatga tggcggactg ttcgccgctg ctactggagc ttaatgagaa cgatccgggt
     1561 attctggtta cgcaatctgt gcataaacaa caggctggtt tttctcagac ttcacaaatt
     1621 cataaaaaag acagccatat caaagggcaa cagcgttatg taccgcacaa acgcatgaac
     1681 aacgcattta tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat
     1741 atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggtt
     1801 aacggtatta atgcccgcaa attgatcctc gataactgcc agcacattcg cccgttcgtt
     1861 cctgaactgg tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat
     1921 ctgcgcttct ttaaatttgt acccggtgag cactggcatt cttttgaagg ctacgcagag
     1981 aaccaatact ttgtcgatcc gtgcaaactg ttgctgacaa ctccaggtat tgatgcacgt
     2041 aacggcgaat atgaagcgtt cggtgtacct gcaacgattc ttgctaactt cctgcgcgaa
     2101 aatggcgtag tgccggaaaa atgcgatctt aactccatcc tcttcctgct gactccggca
     2161 gaagatatgg ccaaactgca gcaacttgtt gccctgctgg tacgcttcga aaaactactg
     2221 gaagctgatg cgcctctggc ggaagtactg ccttccatct acaaacagca tgaagagcgc
     2281 tacgccggtt ataccctgcg tcagttgtgt caggaaatgc atgatttgta tgcccgtcac
     2341 aacgtgaaac aactgcaaaa agagatgttc cgtaaggaac acttccctcg cgtcagcatg
     2401 aatccgcaag aagccaacta cgcctactta cgcggtgaag tggagctggt tcgtctgccg
     2461 gatgcagaag gccgtatcgc tgccgaaggt gcgcttcctt atcctccggg tgtgctgtgt
     2521 gttgttcctg gtgaaatctg gggtggtgct gttctgcgtt acttcagcgc tctggaagaa
     2581 ggcatcaacc tgttgccagg ttttgcaccg gagctgcagg gtgtctatat cgaagaacat
     2641 gatggtcgta agcaagtttg gtgctatgtc atcaagcctc gtgatgcgca aagcgccctg
     2701 ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggtgtc gttcagttaa
     2761 ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg acaaagcttg
     2821 ccgaagtcgg gacaatctca attatctcct ggctggtgac agccgtaggc tcaatggcac
     2881 tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggaggt atgggcggtt
     2941 atgccgagta tgctttcggt aaatccggta actttatggc gaactatacc tacggcgtct
     3001 cgctgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc accgaactgc
     3061 tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggggtg ctgtggattt
     3121 gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc attaccgtgt
     3181 ggggggtcat tattccggtg gtaggcctgt gcattatcgg ctggttctgg tttagcccga
     3241 cgctttacgt tgattcctgg aatccgcatc atgcaccgtt cttcagtgcg gtaggttctt
     3301 ccatcgctat gacgctgtgg gcttttcttg gtctggagtc tgcgtgtgcg aatactgatg
     3361 tagtggaaaa cccggaacgt aatgtgccaa tcgcggtact cggcggtacg ttaggtgcgg
     3421 cggtgattta tatcgtctcc accaacgtga ttgccgggat tgtgccaaat atggagctgg
     3481 caaattcaac ggcaccattt ggtctggcct tcgcgcagat gttcacgccg gaagtgggta
     3541 aagtcattat ggcgctgatg gtgatgtcct gctgcggttc gctgcttggc tggcagttca
     3601 ccattgccca ggtgtttaaa tcttctgccg atgaagggta cttcccgaaa attttctccc
     3661 gtgtgagcaa agtcgatgca ccagtacagg gaatgctgac cattgtcatc atccagagcg
     3721 gattatcact gatgaccatt agcccgtcgc tgaacagtca gttcaacgtg ctggttaacc
     3781 tggccgtggt gaccaatatc atcccgtata ttctgtcaat ggcggcatta gtcattattc
     3841 agaaggtcgc taatgtgccc ccctcaaaag cgaaagttgc aaactttgtt gcttttgttg
     3901 gcgcgatgta cagcttttat gcgctgtact catccgggga agaagccatg ctatacggtt
     3961 ccatcgtgac cttcctcggt tggacactgt atggtctggt ctcaccacgc tttgaacnnn
     4021 nnnnnnnggt taattaagca aagcaaaaaa gggcgatctt ccgaccgccc tttttcttat
     4081 ttgatatgtt tacgcgtttt tcagaacttc gctaacaatc tcaaccgctt ctttctcaat
     4141 ctgcttgcga tgttcttcac caaggaagct ttcgcagtag atcttatatg cgtcttccgt
     4201 gcctgacgga cgcgcggcga accagccgtt gtcagtcatc actttcagac cgccgataga
     4261 agcgccatta cccggtgccg ccgtcagacg cgcggtgatc gggtcacctg ccagggtgct
     4321 ggcgctcacc atttccggag acagcttaga caacgccgct ttttgtgcgg aagtcgctgc
     4381 tgcctgcaaa cggttatagc tcggcgcacc aaagcgtttt gccagttcgt tatagtgttc
     4441 ctgcgggttc ttaccggtga cagcggtgat ttccgccgcc agcagacaca taatgatgcc
     4501 gtctttatcg gtggaccacg gcgtgccgtc gaaacgcagg aaggaagcgc ctgcactctc
     4561 ttcgccgcca aagccgaagc tgccatcgaa caaaccatca acaaaccatt tgaaacctac
     4621 cggtacttct accagcttac ggcccaaatc attgaccaca cggtcgatca tcgcagatga
     4681 aaccagcgtt ttaccgacag caacatcttt gccccactgc ggacgatgct ggaacaggta
     4741 gttgatcgcc accgccaggt agtggttcgg attcatcaaa cctgccggag tgacgatacc
     4801 gtgacggtca taatccgggt cgttagcaaa cgccagatcg aacttatcac gcagtgccag
     4861 caggcccgcc atcgcacact cggaggagca gtccatacgg atcgcaccgt ctttatcgag
     4921 gtgcataaag cggaaggttt gatcgacctg atcgttaacg atggtcaggt tgaggttgta
     4981 atactcgcca atgcgcttcc agtattcgat accggaacca cccagcggat caacgcccag
     5041 cgtcaggccc gctttctgaa tcgccgccat atcaacgata tcggccagcc cttccacaaa
     5101 cggctgcacc agatcctgct ctttcacatg accggatgcc atcgcttcgt cgagggagat
     5161 acgcttcacg cctttcaggc catcagccag cagtgcgttg gctctgtctt ccaccacttt
     5221 agtgacgttg gtatcagccg ggccaccatt tggcggattg tacttgatac caccatcttc
     5281 cggcgggtta tgggacggtg taatcacgat accgtctgcc agcgggccac cttttttatt
     5341 gtgaaccaga atggcattgg aaatggcagg tgtcggagtg aagccattgt tttcctgcac
     5401 aatgacatca acgccgttcg ctgccagcac ttccagtacg gaaatgaacg caggctcgga
     5461 cagggcgtga gtatctttac ccacatagca agggccagtg atgccgtttt tcgcacgttc
     5521 ttcagcaatt gcctgagcga tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc
     5581 actgccacgg tgaccggaag taccgaattt caccgcgtgc tccgcattcc ctgcttctgg
     5641 tttcagtaca taatattgcg ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc
     5701 aggttggcct gcacgattgt ggattgccat tgctttgtcc tttgtctgca acgttttaga
     5761 tagttccgca aaccttctca atcaattccg ccgggaattg catcgactgc atgatgtgtt
     5821 cgatcatgct gcatttacgg ccggtgttgg tgttggtgat cacccaatac ggcgtaccag
     5881 gcacatgttt cggcttggtc tgattaccat ttttcagcag cgtttgttca tctgccgcaa
     5941 agtaaacgcg tgtacgaccg tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa
     6001 gagaatatag tgtagacaac agcagcataa agcgattgac cgctcgcttt tgctctgcgt
     6061 attcgtccga aagcagaagt tcacgcattg cgcgaacctt gtctttaatc gttttgaccg
     6121 gcttcgcttc gacgatagca ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag
     6181 gctgtgatgc ggcggaaaat ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga
     6241 tatgcttagt gtggctggca atatagctgt agagttcatc atcaacttca atcgttttca
     6301 tcttaatcca gtgcagtgtc ttagctgaat acaagtcgcc aggagtatat gaataaatcc
     6361 cggcagcgga tagcgtcaag cctggcttgc agaaaaactc ggaataaacc ttatttgttg
     6421 cagccctgtg gcacaatggt caacataata ccctaacccg acaacgcgaa aaaaagaact
     6481 ttgccatgaa attgaatatc cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca
     6541 tcgttcttgt ccatggtctg tttggcagcc tcgacaacct tggcgtactg gctcgcgatc
     6601 tggtaaacaa tcacaatatc atccaggttg atatgcgtaa cctcggtctt tcaccgagag
     6661 atccggtaat gaattacccg gcgatggcgc aggatcttgt tgataccctg gatgcacagc
     6721 agatcgacaa agcaacattt atcggtcact ccatgggtgg taaagcggta atggctctta
     6781 ctgcactagc ccccgatcgc attgacaagc tggtggcgat tgatattgcg ccggtcgatt
     6841 atcacgttcg ccgtcatgat gagattttcg cggccatcaa cgcagtcagt gaatcagacg
     6901 cacaaacacg ccagcaagca gcggcaataa tgcgccagca tcttaatgaa gaaggggtga
     6961 ttcagtttct gttgaaatct tttgttgacg gggagtggcg ctttaacgtg ccagtattgt
     7021 gggatcagta tccgcatatt gtgggttggg agaaaatccc ggcatgggat catcctaccc
     7081 tgtttatccc tggcggcaat tctccgtatg ttagcgagca gtaccgtgat gatttactgg
     7141 cacaatttcc acaggctcgg gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg
     7201 aaaaaccgga tgcggtatta cgcgctatcc gtcgctatct caatgattaa cgcaactgat
     7261 taaaaaccca gcgtccgcgt cgctttccgc gcgtggacgt tggcgtgact catcgcgctg
     7321 atgtattatg gcgcgctatc catccgggca ggcgcctggc tgattgtttc ccccgaagtc
     7381 accaagatca tggccaaaga acaaacggac cgtacgacat tagatctgtt cgcgcacgag
     7441 cgtcgaccgg gacgaccgaa aactaatccg ctttcgcgcg atgagcagct gcgtattaat
     7501 aaacgcaacc aactaaaacg cgacaaagta cgtggcctta agcgtgtcga actgaagctg
     7561 aacgcggaag ctgtcgaggc actgaacgag ctggcggaat cgcgcaatat gagccgtagc
     7621 gaactgatcg aagagatgtt aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt
     7681 taaatttcca ctttcatgta gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga
     7741 ctgcctgttc tgctatgatt gcctttatcc gtgggcaatt ttccaccccc atttcaataa
     7801 gtttcaagag gttatttcac tcatggctat cactggcatc tttttcggca gcgacaccgg
     7861 taataccgaa aatatcgcaa aaatgattca aaaacagctt ggtaaagacg ttgccgatgt
     7921 ccatgacatt gcaaaaagca gcaaagaaga tctggaagca tatgacattc tgctgctggg
     7981 catcccaacc tggtattacg gcgaagcgca gtgtgactgg gatgacttct tcccgactct
     8041 cgaagagatt gattttaatg gtaaactggt tgcgctgttt ggttgtggcg accaggaaga
     8101 ttacgcagaa tacttctgcg atgcattggg caccatccgc gacatcattg aaccgcgcgg
     8161 cgcaactatc gttggtcact ggccaaccgc gggctaccac ttcgaagcgt caaaaggtct
     8221 ggctgatgat gaccactttg tcggtctggc tatcgacgaa gatcgtcagc cggaactgac
     8281 cgctgaacgt gtagaaaaat gggttaaaca aatttctgaa gagctgcatc tcgacgaaat
     8341 tctcaatgcc tgatgtgatg cggcgtagac tcatgtctac gccgtattaa tagataatgc
     8401 caatcaaaat aattgctaca aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg
     8461 tgttcaagtg gccttgccgt tgtaaatgta agctgtgcca cgtttttatt aacaatattt
     8521 gccagggact tgtggttttc atttaggcgt ggcaattcta taatgatacg cattatctca
     8581 agagcaaatt ctgtcacttc ttctaatgaa gtgaaccgct tagtaacagg acagattccg
     8641 catgactgat aacaataccg ccctaaagaa agctggcctg aaagtaacgc ttcctcgctt
     8701 gaaaatcctg gaagttcttc aggagccgga caaccatcac gtcagtgcgg aagatttata
     8761 caaacgtctg atcgatatgg gtgaagaaat tggtctggct acggtatatc gcgtactgaa
     8821 ccagtttgac gacgctggta tcgtcacccg ccacaatttt gaaggcggta aatccgtatt
     8881 tgaactaaca cagcaacatc accacgatca cctgatctgc ctcgactgcg gtaaggttat
     8941 cgaatttagt gatgattcca tcgaagcgcg tcagcgtgaa atcgccgcaa aacatggcat
     9001 tcgcctgact aaccacagtc tctatcttta cggtcactgt gccgaaggcg attgccgcga
     9061 agatgagcac gcgcacgaag gcaaataagc cagcctgaac gagaaaagcc aacctgcagg
     9121 ttggcttttt tatacaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt
     9181 attactgcga atttcctgcc agaccttatc acaatcggct ttcaccgcct ggtcattacc
     9241 ggtttgcgtt gcgcgcagac actgctgata atccagtacg cggacacttt cctggtcagc
     9301 aaactgctga tgttgtctct ctttcttcag cacgtttaac acgctctggc aggcttcaat
     9361 tttttccggc gaaccttgcg cggtgttgat acaggcgctg taagcctctt tcagacggct
     9421 gtcttcctgc ggtgcggttg attgcgcaca agctaccagc cccgatgcca tcatggcgat
     9481 gagaatcagt tttttcatag caaattcctc aaacgtgcgg caggcacaaa acctgccgcg
     9541 tcgggcatca gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgttcatcct
     9601 ggaagatgtt gccgtaacca ccaccccagc ttgggatgtc ggagtggttg tcgtattcgg
     9661 tgaagtgcag tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tacaccgcat
     9721 ccaggctgta ggcagactct tcaatagtcc ggtttttatc gtagtacgca tccggattgc
     9781 tctgccaggt cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc
     9841 ctggaagatt ccagtttttc aggtcataca tcgcaccgaa gaagaccgct ttttcgccgt
     9901 tggcgttgaa gtcggaacgg ttatcccacc agatatccag gcgaccgttc gaggaagcgt
     9961 aggttggagt catacgttgc aggaagtatc cttgctgacc gtcagcctta acccaggtac
    10021 cttcgaggcg caaatccact acgtcagccg cccggtaacc aaaggtcaac gcctgcagcc
    10081 aggcggtgcc gtcataaaga tcgttgatgc tgcgatcgtc aactttatcg cgcgtaccat
    10141 agaactggta gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg
    10201 caaaatattg atcgatatac ccttcggcct gaccaaacgc cgcttccagt acgaagttat
    10261 ttttgaagtc gtacttcgcc ccaagggagt gtagataatc aactttggtg gttttatcgt
    10321 tctgataaaa ctcatccatt tccagatgcc acggtgcttt gtattcgttg gtccacatgt
    10381 aggagaaact caacgcacca gcatcgccgt aatcaaaatt cgccccggcc tccgccccct
    10441 gataagtacc cggcataaag ctccagtgtg gcgctaacag cgtctgaccg gttggctgaa
    10501 tataacctgc cctcgcccaa accggaccgt atttaaattt ggccgctgct ttatacaggc
    10561 ttataccgct tttatcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg
    10621 cgatttcgtt cgggtgagag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcgg
    10681 caatatcaag gccgaacata tcagcagcat aaccagactg aaaatcgagg ttggcgttcc
    10741 aggtggagtg agaaaggttg gttttgtatt tatcgccgtc ggtaacatct ttacggtcgc
    10801 gttcacgctg ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg
    10861 cgcgagcctc cggaattgcc ataaagcccg acatagctgt aacaccggcg atagccagcg
    10921 ccagcgtact acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat
    10981 tgctgcacca aaggtgaaca gcgaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag
    11041 tctcgctgac tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta
    11101 accagaatgt aagaacatga gcaaccgcac atattttgat gatttttgtt acaaggctta
    11161 agtttgcagt tgaaaggctt gcagaagaat aaaagcgtga atttccagca agatgaaaac
    11221 ggttacattt tatttaatta tggtttgtga ttttaaattt gttttatgga taaattg
//
LOCUS       DAWDRC010000048        11230 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_2996_length_11230_cov_2.894049, whole genome shotgun sequence.
ACCESSION   DAWDRC010000048 DAWDRC010000000
VERSION     DAWDRC010000048.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2996_length_11230_cov_2.894049"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4514..4523
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2948 a         2871 c         2660 g         2741 t
ORIGIN      
        1 aatataaata aattttgctt gattcatgca agcggcatta atactattta tactaacgtc
       61 aatatacaac ccacccaatc tattttatta gaacatacat cgtgcccaca ctacctttcc
      121 ccttccatct ggcgaatcat aataaagatg ctgccatcaa tatattgatt acatttataa
      181 gcatagggct gattattttt aatgtgaatt atttccatac aactatagtt aaaaatgaac
      241 agatcattaa tcaaccaaca aatgcatttc agtctgaatt cagccttgcc gctttatggc
      301 gcaacgaaaa tcatgctggg gtaaaagatg caaatcccgt ggcagttaat caggaaacac
      361 ctaagctctc tatagcgctt aatggcattg tactcacatc gaatgatgag acttcttttg
      421 tcttaattaa tgaggggaac gaacaaaagc gatactccct caacgaagcc cttgagtcag
      481 cgccgggaac gtttattaga aaaataaata aaaccagcgt tgtgtttgaa actcacgggc
      541 attacgaaaa agtaactctc catccagggc tacctgacat catcaagcag cctgattcgg
      601 aaaatcaaaa tgtactcgcc gattatatta tcgccacacc tatacgtgat ggagaacaga
      661 tatacggtct tcgattgaat cctcgtaagg gtctcaatgc atttaccacc agcttgctgc
      721 aacctgggga tatcgccctc aggatcaata atctttcgct gacccaccct gatgaggttt
      781 cacaggcatt aagtttactg ctaactcaac aaagtgctca gtttacaatt cgtcgcaacg
      841 gcgtaccccg cttgataaat gtttccgtcg cggaacttac aggaatgaat ggacagcgca
      901 atgaagggac tcaataaaat cacctgctgc ttgctggcag cactactcat gccttgtgca
      961 ggacacgctg agaacgaaca atacggcgct aacttcaata acgccgatat ccgccagttc
     1021 gtggaaatag tggggcagca tcttggcaaa acgatcctga tcgacccttc ggtacaggga
     1081 accatttccg tacgcagtaa tgatacgttt agccaacagg agtactacca gttcttttta
     1141 agtattcttg atctttacgg ttattccgtg atcacactgg acaatggttt tctgaaagtg
     1201 gtgcgctcag ctaatgtaaa aacatcgcca gggatgattg ctgacagttc tcgtccaggc
     1261 gtaggtgatg agttggtcac ccgaatcgta ccgcttgaga acgttcctgc tcgtgacctg
     1321 gctcccctgc tccgccagat gatggatgcg ggtagcgtcg gtaatgttgt gcattatgaa
     1381 ccctccaacg ttcttattct gaccggtcgt gcctccacca ttaataaact gattgaagtc
     1441 ataaagcgcg ttgatgtcat cggcacagag aagcagcaaa ttattcatct ggaatatgca
     1501 tcagcggaag atctcgccga gattcttaat caattaatca gcgaaagcca cggtaaaagc
     1561 cagatgcctg ccctcctttc cgcgaagatt gtggcggata agcgaaccaa ctctcttatc
     1621 atcagcgggc cggaaaaagc acgccagcgc ataacttcat tactgaaaag ccttgatgta
     1681 gaagagagcg aggaaggaaa tacccgggtc tattacctga aatatgctaa agccacgaat
     1741 ctggtggaag tgctaaccgg tgtttccgaa aagctgaaag atgaaaaagg gaattcgcgt
     1801 aagccttctt ctacttctgc gatggataat gtggccatta ccgccgatga acagaccaac
     1861 tcgttggtca ttaccgctga ccagtccgtc caggagaaac tcgccacggt aattgcgcgt
     1921 ctggatattc gccgtgcaca ggtgctggtt gaggcaatca tcgtcgaagt tcaggatgga
     1981 aatggactca acctcggcgt gcaatgggcg aataaaaacg ttggcgcaca gcaatttacc
     2041 aatacgggat taccggtttt taacgctgcg caaggtgtag ctgattataa aaagaatggc
     2101 gggatcacca gcgcgaatcc tgcctgggat atgtttagcg cctacaatgg catggctgca
     2161 ggcttcttca atggcgactg gggcgtattg ttgactgcgc tggccagtaa caataaaaat
     2221 gacatcctcg ccaccccgag catcgtaacg ctggataata aactcgcgtc cttcaacgtt
     2281 ggtcaggatg tgccggtgct atccggctct cagaccactt ctggggataa cgtctttaat
     2341 actgtcgaac gcaaaacggt gggcacaaaa ctcaaagtca ctccgcaggt caatgaaggc
     2401 gacgcggtac tgctcgaaat agagcaggag gtttccagcg ttgactcttc gtctaattcg
     2461 acgctcggcc cgacatttaa cacccgtact attcaaaacg ccgtgctggt taaaaccggt
     2521 gaaacggtgg tcctgggcgg attgctggat gatttttcta aagagcaagt atcaaaggtt
     2581 cctctgcttg gcgatattcc tttagtggga caactcttcc gctatacctc gaccgagcgc
     2641 gctaaacgca acctgatggt atttatccgc ccgacgatta tccgtgacga tgatgtttat
     2701 cgctcactgt caaaagagaa atacacccgc taccgtcagg agcaacagct gcgaatcgac
     2761 gggaaatcaa aagcgctggt tggctcggaa gatttgccgg tgctgaatga gaacacgttc
     2821 aacagccacg cccctgcgcc atcgtcacgg taaggcattc ttatgagaat tcactcaccg
     2881 taccccgcca gttgggcgct ggcacaacga attggttatc tctattcaga gggcgagatt
     2941 atttatctcg ccgatacgcc attcgaacgg ttactcgata ttcaacgtca agttggccag
     3001 tgccagacca tgaccagctt gtcacaggct gattttgaag cccgactgga agcggtgttc
     3061 catcagaata ccggtgagtc gcaacagatt gcgcaggata tcgatcaatc cgtcgatctt
     3121 ctctcgcttt cggaagagat gcccgcaaat gaagatctcc tgaatgaaga ttcagcggca
     3181 ccggttatcc gcttgatcaa tgctattttg agtgaggcca tcaaagaaac cgcctccgat
     3241 atccacattg aaacctatga aaaaacaatg tcgatccgtt ttcgcatcga cggcgttttg
     3301 cggacaattt tacagcccaa caaaaaactg gcggcactgc ttatctcccg aattaaggtc
     3361 atggcgcgtc ttgatatcgc cgaaaaacgt attccacagg atggaagaat cagtttgcgt
     3421 atcgggcgac gtaacataga tgtccgcgta tccacactgc catccatcta tggtgaacgt
     3481 gccgtgctcc gcctgctgga taaaaacagc ctccagcttt cattgaacaa cctggggatg
     3541 acggcagcgg ataaacagga tttagaaaat ctcattcagc ttccgcacgg tattatcctg
     3601 gtaacagggc cgacaggctc cggtaaaagc accacgctct acgccattct ttcggcgctt
     3661 aatacccctg gccgtaatat tctgacggta gaagatcccg tggaatatga actggaaggc
     3721 ataggacaaa ctcaggtgaa tacgcgcgtg gatatgtctt tcgctcgcgg cctgcgcgcc
     3781 atacttcgcc aggacccgga tgtcgtcatg gtgggggaga ttcgtgatac agaaaccgcg
     3841 caaattgcgg ttcaggcctc gctcacaggc catctggtac tctcaacact ccacactaac
     3901 agtgcatcag gcgcagtcac ccggctacgc gacatgggcg ttgaatcatt cctgctttcg
     3961 tcttcactgg cagggattat cgcgcaacgt ctggttcgtc gtctgtgtcc gcaatgccga
     4021 caattcacgc ccgtatcgcc ccaacaagcg cagatgttta aatatcatca gctcgcggtg
     4081 acaacaattg gcactcccgt aggttgccct cattgccatc aatccggtta tcaggggcgc
     4141 atggcgatcc acgaaatgat ggtggtgacg ccggaattgc gggccgctat tcatgaaaat
     4201 gtggatgaac aagcactgga gcgactggtc cggcaacaac acaatgcctt aatcaaaaat
     4261 ggcctgcaaa aagtgatacg cggtgacacc tcctgggatg aggttatgcg cgtcgccagt
     4321 gccacgctgg agaacgaagc atgaattatc gctatcgcgc catgaccctg gatggtcaaa
     4381 aattgcaggg gatcattgat gctaacgatg aacgtcaggc acgactgcgg ctgcgtgaag
     4441 aagggctttt cctgttggat attcgccccc aaaaaagttc gggagtaaaa acacgtcgcc
     4501 cgaggataag ccannnnnnn nnnactgacg cttttcaccc ggcagttggc aaccttaagc
     4561 gcagcggcat tacccctgga agagagcctt gccgtaattg gtcaacaaag cagtaataat
     4621 cgactggctg acgtgttaaa tcaggtacgc agcgccatcc ttgaagggca tcccctttcc
     4681 gatgcgttac aacattttcc cacgcttttc gattcgctct accgtaccct ggtaaaagcg
     4741 ggcgaaaaga gcggtctgct ggccccggtg ttggaaaagc tggctgatta caatgaaaac
     4801 cggcagaaaa tccgcagcaa gctcattcag tcactgatct acccctgcat gctcactacg
     4861 gtggcgattg tggtcgtgat tattctcctc actgctgtcg tgcccaaaat taccgaacag
     4921 tttgtgcata tgaagcagca actgccactg agtacacgca ttcttttagg tctgagcgac
     4981 acgttgcaac ataccggccc gacattacta gcgacagtgt ttattgtcgc tgtaggtttc
     5041 tggctctggt taaaacgcgg caataaccgc caccgttttc atgccatgtt gctgcgcgtt
     5101 gtactcatcg gcccgctgat ttgcgccatt aacagcgcac gctatctccg cactttaagt
     5161 attttgcaat ccagcggcgt ccctctgctg gatgggatga atttgtccac cgaaagtctc
     5221 agcaacctcg aaatccgcaa gcgtctggca aatgcggcag aaaacgttcg gcagggtaac
     5281 agcattcatc tttcgctgga acaaaccgca atcttcccgc cgatgatgct ctacatggtg
     5341 gcctctggcg aaaaaagcgg gcagctcggc acattgatgg tcagggccgc agataaccag
     5401 gagacactcc aacaaaatcg gatcgcctta acgctctcca tcttcgagcc cgcactcatt
     5461 attacgatgg cactgatcgt cctgtttatt gtcgtctcgg tactccaacc tcttcttcaa
     5521 cttaactcaa tgattaacta aggaaaaaca atgcgcgcaa cggataagca acgcggtttt
     5581 acattactgg aaattatggt ggtgatcgtc attatcggcg tacttgccag cctggtggtt
     5641 cccaacctga tgggcaataa agaaaaggcg gataaacaaa aagccgtcag cgatattgtg
     5701 gcgctggaaa acgccctgga tatgtacaag ctcgacaatc atcgctatcc aaccacaaat
     5761 caggggcttg aatccttagt cgaagcgccg acacagccac cgctggctgc aaactataac
     5821 aaagaaggtt atatcaagcg tctgcccgcc gatccctggg gcaatgatta tgtccttgtt
     5881 aatcctggtg aacacggcgc atacgatctg ctttcagcag ggctggacgg tgaaatggga
     5941 accgaggacg acatcaccaa ctggggtttg agcaagaaga aaaaataagt cagcgatgaa
     6001 tcagcaacgc gggtttaccc tgctggagat gatgctggtg ctggcgctgg tcgctatcac
     6061 ggcaagtgtg gtgctcttca catatggtcg ggaggatgcc gccagcactc gtgcgcgcga
     6121 aacagcggcg cggtttaccg ctgcacttga attagccatc gaccgcgcca cgctcagtgg
     6181 tcagcctgtc ggtatccatt tctctgattc agcatggcgc attatggtac cgggcaaaac
     6241 accatcagcc tggcgctggg ttccattaca agaagatgcc gcagacgaga gcaaaaatga
     6301 ctggggtgaa gaactctcaa ttcaacttca gccatttaaa cctgatgaca gcaaccaacc
     6361 gcaggtggtg attctggccg acggacaaat cactcccttc tctttgttga tggctaatgc
     6421 cgggaccggt gagccgctcc tgacgttagt cagttcaggc tcatggcccc tggatcaaac
     6481 gctcgccaga gatacccgcc catgaacaaa caatcaggga tgacactgct tgaagtctta
     6541 ctggcgatga gtattttcac cgctgttgcg ctgactttaa tgagcagtat gcaagggcaa
     6601 cgaactgcca tcgagcggat gcgtaatgag acgctggcat tgtggatagc ggataaccag
     6661 cttcagtcac aagattcatt cgacgaggaa aacacgtcca gttctggcaa agagttaata
     6721 aatggcgaag agtggaactg gcgcagcgat atccactcaa gcaaagacgg cacgcttctg
     6781 gaacacacca taacggtgac attacccagc ggtcagacaa cctcgcttac gcgctatcaa
     6841 agcatcaata ataaatccgg gcaggcacaa gatgattaat cgccagcagg gtttcacatt
     6901 gctggaagtc atggcggcgc tggcgatttt ctcaatgctt agcgtgctgg cgtttatgat
     6961 tttttctcaa gcttccgagt tgcatcaacg ttcacagaaa gaaatccagc agttcaatca
     7021 gctacagcgc accatcacca ttcttgataa cgatctcctg caactggtcg cccggcggaa
     7081 tcggagtacg gacaaaatca tggtgttggg tgaagaagcg atttttacca cgcaaagtcg
     7141 cgatccgctg gcccctctca gtgaagcaca aaccttactc actgttcact ggtatctgcg
     7201 aaatcatacg ctctaccgtg ctgttcgtac ttctgtggat ggcaggaagg atcaacccgc
     7261 ccaggcgatg ctggaacatg tcgagagctt tcttctggaa agtaacagcg gagaaagcca
     7321 ggagcttcct ctctcagtca cgttacatct gaagactcag caatatgggg cgttacagcg
     7381 ccgttttgca ttacctgagc agttagcgag agaagagtcg ccagctcaga cgcaggccgg
     7441 gaataataac catgaataat gaacaacgcg gcgtagcact gctcattgta ctcatgttac
     7501 tggcgctcat ggctgctctg gctgcagaca tgacgatcag ttttcacggc cagcttcatc
     7561 gaacccgcca ggtaaaccat catttgcaac ggcaatatga tattgagctg gcggagaaac
     7621 ttgcgcttgc cagcctcacc ctggacgtta aagataacga cagacaaacc acgttgcagc
     7681 agtactgggc acaaccgcaa cagttgcagc tggaaaatgg caatacagtg aagtggcagt
     7741 tacgcgatgc ccagcactgt tttaatctca atgccttagc aaaaatctct gacgccccgc
     7801 tggcttcccc tgatttcccc gcccaggtat tcagtgccct gctgattaac gccggtatcg
     7861 atcgcggaaa caccgatgag atcgtgcaat caattgctga ctatattgat gccgatgatt
     7921 cgccacgctt tcacggcgca gaagataact tttaccaaag ccaaacgccg cctcgccata
     7981 gcgccaatca gatgcttttt ctgaccggtg aattgcgtca gataaaaggc ataaccgaaa
     8041 acatctatca gcggcttatc ccgtatgtct gcgttctccc gacctcggaa cttagcatca
     8101 atcttaatat gctaacggag aacgatattc cgctcttcag agcgttattc ctcaataaca
     8161 tcacggatgc tgacgctcgg gtcttattac aaaaaaggcc cggggaaggc tggctaacca
     8221 cagatgcatt tctttactgg gcgcagcagg atttctccgg cgtgaagccc ctggttgctc
     8281 aggtgaaagg gcatctcttt ccctatagcc gctattttac gctgagtacg gaaagtatca
     8341 gcgacgaaca atcccaggga tggcaaagcc atattttttt caaccgtaaa cagcagagcg
     8401 cccaaatcta tcggcgtacg ctgcaactct actaatcata aggatcgtga tgcctgaatc
     8461 gttgatggta atacgttctt tctcgacgct gcgtaagcat tgggaatgga tgacgttctc
     8521 cgcagatagc gtatcttcgg tacatacgct cacagacgat cttccgctgg agtcgctggc
     8581 cgatcagccc ggtgctggaa atgttcacct tttgatccca cctgagggtc ttttatatcg
     8641 ttccctcacg ctgcccaacg ccaaatacaa actcaccgcg caaacattac agtggttggc
     8701 ggaggagaca ttacccgacg ccagccaaaa ttggcactgg acggttgtcg ataaacaaaa
     8761 cgaaagtgtc gaggtgatag gcattcagtc ggagaaactc agtcgttatc ttgagcgttt
     8821 acatactgcc ggactcaacg tgacgcgggt tttgccagat ggctgctatc ttccctggga
     8881 agtagatagc tggactctgg ttaaccagca aacaagctgg cttattcgca gtgccgcaca
     8941 tgccttcaac gaactggatg aacactggtt acagcatctt gctaaccagt ttccgccaga
     9001 gaatatgcgt tgttatggtg tagcgcctca cggcgttgca gcggccaatc cacttatcca
     9061 gcatcctgaa ataccatcac tgagtttgta ttctgccgat attgcattcc agcgctatga
     9121 catgctgcat ggtgtttttc gtaaacagaa aactgtaagc aaatcgggaa aatggctcgc
     9181 ccgcctggca gtaagctgtc tcgttctcgc cattctttct tttgttggca gccggggcat
     9241 cgcgttctgg caaacgccta aaatttaaga tcaacttcag caacaacagc aagaaacctg
     9301 gcaacgttat ttcccgcaga tcaaacacac ccacaacttt cgtttttact ttaagcagca
     9361 actcgctcag caatatcctg aagctgtacc gttgctctat catttacaaa cacttctgct
     9421 tgaacaccct gaactgcagt taatggaggc gaactatagc cagaggcaaa agtcgctgac
     9481 cttaaaaatg agtgctaaga gtgaagccaa tatcgatcgt ttctgtgagt taacacagtc
     9541 ctggcttccg atggaaaaaa cagaaaaaga tccggtcagt ggtgtatgga cagtaaggaa
     9601 ctcagggaaa tgattaaatc atggtgggca gaaaaatcca catcggaaaa acagatcgtt
     9661 gcggcattgg cggttctctc gctcggcgtt ttttgttggc ttgcggtgat aaaaccgata
     9721 gatacctaca ttgaagagca tcagtcccac gcgcaaaaaa ttaagaagga catcaaatgg
     9781 atgcaggatc aagcgagcac ccttggttta ctcggtcatc cagcgcttac gcagcccatc
     9841 aaaaatatcc ttctcgaaga agcaaaacgt gagaatttga ctatcacgct ggaaaacggt
     9901 ccggacaata ctctgacaat taaccctgtc acagctcccc tcgaaaacgt ctcccgctgg
     9961 ctcactacgg cacagataac atacggtatt gttattgaag accttcagtt tactcttgcc
    10021 ggaaacgaag agatcaccct caagcatttg tccttcaggg agcaacaata atgacaatgc
    10081 tactgccgct cttcattctg gttggtttta ttgcaggtta ttttgttaat gtcatggcct
    10141 atcacctctc gcctttggag gataaaacgg cgttaacgtt tcgccaggta ttggttcatt
    10201 tctggcaaaa aaaatatgcc tggcatgata cagtgcccct gatactttgc gttgctgctg
    10261 ctatcgcctg cgccctggca cctttcacgc ccattgtgac tggcgcactc tttctctatt
    10321 tctgtttcgc actcacgctc agtgttattg attttcgcac tcagctcctg cccgacaaac
    10381 tcaccttacc gctgctctgg cttggcttgg tatttaatgc gcagtctgga ttaattgatt
    10441 tacatgatgc ggtttatggc gcggtcgctg gctatggggt gctgtggtgt gtttactggg
    10501 gcgtctggtt agtttgtcac aaagagggat tgggctacgg tgatttcaag ctactggccg
    10561 ctgcaggcgc gtggtgtggc tggcaaacgt taccaatgat actgctgatt gcctcgctgg
    10621 gtggcattgg ttacgccatc gtttcacaac ttctgcaacg ccggactata cccactatcg
    10681 cattcggccc gtggcttgcg ctcggcagca tgataaacct gggatatctg gcctggatct
    10741 cttattaacc gggagggttc tccctcccga cacggctcaa ccttcttcgc gaatctgtgc
    10801 ttgcagataa ttttgcagtc ccatcttctg aatcagatca agttccgttt ccagccagtc
    10861 gatatggcct tcttcatcgc gcaaaatttc tatcatcata tcgcggctga cgtaatcatg
    10921 aacgctatcg gcataaccaa ttgcctcacg caaattcttc gcgccatcca gctcaagtgc
    10981 cagatcagaa cgcagcattt cctcaacatc ttcaccaatg ttcagtttgc ccaggtcctg
    11041 taagtttgga agaccttcca gaaaaagaat gcgctcaata taacgatcgg cgtgtttcat
    11101 ctcatcaatg gattcatgat actccacatc attgagacgt ttgagacccc agtttttaaa
    11161 catccgggca tgaagaaagt actgattgat tgcgacaagc tcatttccca acagtttgtt
    11221 gagataattt
//
LOCUS       DAWDRC010000049        11165 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3024_length_11165_cov_2.199010, whole genome shotgun sequence.
ACCESSION   DAWDRC010000049 DAWDRC010000000
VERSION     DAWDRC010000049.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11165)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11165)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3024_length_11165_cov_2.199010"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2435..2444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5536..5545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6208..6217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8736..8745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10743..10752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2766 a         2915 c         2980 g         2454 t
ORIGIN      
        1 gtatcggctg cgaaactgcg cagttcccgt taccgcaggt gggtaaagat ctgttccgcg
       61 atctgaaacg cgtcgcgcaa acgctggata gcattaacgg cggcgaagcg tatcagaaag
      121 tgtgtgatga actggtcgcc tgcttcgata atcctgatct gactttctct gcccgtatct
      181 taaggtctat gattgatact ggtattggcg gaacaggcaa agcgtttgct gaagcgtacc
      241 gtaatctgct gcgtgaagag ccgctggaaa ttctgcgcga agaggatttt gtcgccgagc
      301 gcgaggcgtc tgaacgccgt cagcaggaaa tggaagccgc tgataccgaa ccgtttgcgg
      361 tgtggctgga aaagcacgcc tgacagaaaa gaaaaaggcc actcgtgagt ggccaaaatt
      421 tcatctctga attcagggat gatgataaca aatgcgcgtc tttcatatac tcagactcgc
      481 ctgggaacaa agagttcaga aaatttttaa aaaaattacc ggaggtggct aaatgccgtt
      541 gttagatagc ttcacagtcg atcatacccg gatggaagcg cctgcagttc gggtggcaaa
      601 aacaatgaac accccgcatg gcgacgcaat caccgtgttc gatctgcgct tctgcgtgcc
      661 gaacaaagaa gtgatgccag aaagagggat ccataccctg gagcacctgt ttgctggttt
      721 tatgcgtaac catcttaacg gtaatggcgt agagattatc gatatctcgc caatgggctg
      781 ccgcaccggt ttttatatga gtctgattgg tacgccagat gagcagcgtg tcgctgatgc
      841 ctggaaagcg gcaatggaag acgtgctgaa agtgcaggat cagaatcaga tcccggaact
      901 gaacgtctac cagtgtggca cttaccagat gcactcgttg caggaagcgc aggatattgc
      961 gcgtaacatt ctggaacgtg acgtgcgcat caacagcaac gaagaactgg cgctgccgaa
     1021 agagaagttg caggaactgc acatttagtc agtttaagca gaagaggccg ggctggtgaa
     1081 aacaccgccc ggcttttgtt acctgtcagt cacccgatac tccacgctat gtgcttcatc
     1141 aagttgagga acttccagcc ttgaggccat ccgcatcatg accgcttcag gaactgcata
     1201 ctcgcgtctg gcattttgct gcctccattg cgcccatggc acttccagat aaacgatttt
     1261 tactcgtgcg ccgtaagccg taaacagact gatgagctgg ctgcgtagct ggcgggtaat
     1321 gttcgttgca ttccagatga acggcttttt ctggcgtaaa aaaacgcgcg cctcttcttt
     1381 tgcctgttgc acaatacgcc cggtagcggt tttgtcgtcc ggactggcat tgattcgccg
     1441 ccgcatatca tcaaggctga taacgtccat tccctggcac tgttcattga tatagcgatc
     1501 tttgcccata cccggtaagc cacacagcaa aatgacctca aaaggttcag cctcccaggg
     1561 aacaaaatca ggagaacttt gctcatgggt caggtaatgc cagcgtgcgg aatcagaaac
     1621 gaacgggcgc atttttcccc agcattgttg ctcatggcaa aacagctcga acagatcgat
     1681 gcgttccagc attgattgct gatccgggct ttggcgacca agcagatcgg cacgagcaag
     1741 caaagcgagc agacgcgtgt cgatgcgcat tgcagccgtg agcaacaaac gctccggttc
     1801 cgggcgttcc agtaaccata gcggtaaccc atgcagacgc accagtgcaa caatttgctc
     1861 gcgcagcacg aacggcgttg ggatatcgcg ccataaaatt tgtcgggccg tcagttcgcc
     1921 ccgacgagca tgaccaggag actgaatacg tccgttttcc tgcacggtag tgctgcgctt
     1981 ttcaacatca tgcaataacg ccgccgccca taaaacttcc tgctgttgag cggggagttg
     2041 ctggaattct ggcagagtaa tgagggcgtt aagcaccatt tcggtatgta cgccgacgtc
     2101 gccttcacca tgatgctcag ggtcttgtgg cgtgtgatgc atctcctcaa cccagctaaa
     2161 acgctgacgt agcgccgacc agcgtttatc gtcggtaagt tgccaaatca tgactgttct
     2221 ccataaacga actcatgggc catccgcgcc cgttgccagt ggcgtttcca gtgcacagtg
     2281 gttttgacat gatttttgcg cacatacttg aagacgttat gcgaaaagtc agcgacagaa
     2341 aacgcatcat gatttcggct gacaatgcct tccagcgtac agggttgaca ggtttgcgta
     2401 tcccagggat cgaatgtgcc gcgcgcaatg gtcannnnnn nnnncgccag aaaatcacgt
     2461 tgccagcttt tttcatcatt ccccggctgc gggccggaga tttcgggaac gcaggggaaa
     2521 tcaaacagag cggcgtaaaa ctgcacctct tcccagctca gccacatatc ctggcagcga
     2581 acggcaaaca ggtaaaagtc ctgttccagc gcgcggtatt caatggaatg aacggcgtag
     2641 agattttcac caaacaactc caggtcgccg agatcgtttt tcagcagttg ccagcgttgg
     2701 cgaattttat aggtccatgc cgactgcgta ggcgtggcat gggagcgggc aaacacgccg
     2761 tagcggttaa ggcagttatt ttcgccatcg agtttttctg tgtgcaccaa ttgcgtgatg
     2821 gtctgtaaat cctgccagta atcggtgtta atgcggtcat cgctggtggt gcctggagag
     2881 aaggggtaat gccaggttct cccgtattta cgttgggtat tcataataat ccattatttt
     2941 gtgtgtaata aatgtacgcc cgcgagggtg cgagttttgg ggttaaaaga aaaatgtgag
     3001 tatcacccgc gaaagcgagg gcgaaggaaa aagagaatta acaacctgag cgaatgctca
     3061 gaaccggcta atggtgaatg gaagagtttg gggcggcgaa aagatacacc gaatgagtgt
     3121 ataaaacaac cgtttgcaga atgagcaaat aaaaagggag ccgcagctcc ctttggcaac
     3181 cttagtgcgc accgcctccg ccgccacctg cgccaaatgg cggtttagca aaccacacca
     3241 gccccagcag gacgaggaat atcccggctg acatccagaa gatctcattg gcggaaataa
     3301 tcagcccctg attggttatc tgctgggcaa tccagcctga tgcctgctgt tgcgtcatac
     3361 caagcccttc cagttgactg tacatcgcct gagcattcgg gttgaacggg tttaccgact
     3421 cagtcaactg tgcatggtgc agcgactcgc ggttggtcca catggtcgtg gttatcgacg
     3481 tgccgataga ccccgccagc gttcgcgtaa agttggagag gctcgatgcc gccgccagtc
     3541 gttccggtgg caaaccggac agcgtaatgg tggtcagcgg cataaagaag caggccaccg
     3601 caaaaccctg gataaactgt ggccaggccg acgcgccaaa atccataccc ggttcaaagg
     3661 tataggcacg ccagtagaag cagacggcat acataataaa gctgaaggtt accagtcggc
     3721 gcatatccag tttatgcgca aagcggccga taatcggcga caggatcacc ggaataatcc
     3781 ctaccggcgc agaggccagc cctgcccacg tcgccgtgta accgtagacc tcctgcaaca
     3841 actgcggcag cagaacaatc gcgccgaagt agagcatata cgcgaggctg atacacaagc
     3901 agccgatggt gaagttgcgc gacttaaaca acgacagatc gactatcggg ttatcgtcgg
     3961 tcagctccca gacaatcagg aagcagatag ccaccaccgc caccacggtg aggatgataa
     4021 tttcctgtga tgagaaccag tccagctctt taccgcggtc gagcataatc tgcaggctgc
     4081 cgataccaat aaccagcagt gccagcccca cggcatcaat ccgccgccgt tcggtgcggg
     4141 tttcacgtcc gcgcagagtt tgcagtgtca tcaacaccac cgccacgcca atcggcacgt
     4201 tgatgaagaa tatccagccc cagtggtaat tatcactgat ataaccgccg aggatcgggc
     4261 cgcaaattgg tgcaacaatc accgtcatcg accacaacgc cagcgcgatc gagcgtttgg
     4321 ctggcgggta gttattcagc aatagacttt gtgaaagcgg gatcagcggc ccggcgacaa
     4381 tcccctgaat cacgcggaag aagatcagca tattcaggct gctggagaca ccacacgccc
     4441 acgacgcaat agcaaaggcg atggttgacc agaggaacaa tttcacttcc ccgacgcgct
     4501 ttgccagcca gccggtaagc gggatcgaga tggcattcgc caccccgaaa gaagtgatca
     4561 cccacgttcc ctgactgagt gatgagccca gattcccggc gatagtgggg atcgccacgt
     4621 tagcaatggt ggagtccagc acctgcatga atgtcgccag tgacagcgca atcgtcataa
     4681 tgaccagttg tgcgccttcc agcggttttt gctgttgcat cacacgcacc tctggattag
     4741 ccagcgttag cttttacgat atcgtcgatc agtttattga caggtgccag gctgatttca
     4801 cgcgcggtgc ttaccgcgac cggtgtggaa cgtactttat ttgccagtac ctgaccgtca
     4861 cggttagtgg tattgacact caccagcgtg gataaaccga tacgcagcgg gtattgctcc
     4921 agctgtttct ggtccagttc aatacgcaca ggcagacgct gaacgacttt gatccagtta
     4981 ccggtggcgt tttgcgccgg aagcagtgag aacgcgctac ctgtgcccat atccagacca
     5041 accactttac cggtgtattt cacatcatcg ccgtaaatat cggtggtgat ggtgaccggc
     5101 tgaccgatac gcatattggc aatctgcgtc tctttaaagt tggcatccac ccacatattg
     5161 gtggctggaa cgaccgccat cagcggcgtc gttgggctaa tttgcgcccc aggctgtacc
     5221 gcgcggcggg agacataacc ggtcatcgga ctgacaatac gggtacgctc cagcgccagc
     5281 caggcgttac gtacttcggt ggcagcctgt tgcacggcag gctgatcttc cagtttagtc
     5341 cccagaatca tcgcctgatt ggcattgtat tgttgaatag cgacgtccag ttgtgcctgg
     5401 gcactggtaa cggcgtcgcg ggcgtgttgc agctcttcgc gaccaatcag gttggcattg
     5461 cccagcggca cacggcggtt gtagtcgctt tgtgctttcg cgagggcgat tttttgcacc
     5521 tcaatattcg cctgcnnnnn nnnnntcggg tcgagagtga ccagcacgtc gccttctttt
     5581 acaaaatcgg tgttatcggc ccagactttc gtcacgctgc cagacacctg agacataatt
     5641 tgaatctgat tccctgccac gtatgcgtca tcggtttctt cgaaatgacg cagtaccaaa
     5701 aaccaataaa tccctatcgc tacggcaata attataaaga gcaaggtgag aaggaggagc
     5761 agacgcttac gtttgccgct cttctttacc ggttgctgcg gggtttgagt ctccgcattt
     5821 gcgctcatat tgttctccac gatcttctta actcatcggc tgagccgacc tgcttgccaa
     5881 aaaggccagc atgttacgat gctggccggt tattttttct tttataaatc tggatttttg
     5941 agcgagatga cgcgttagct catcgcttcg agaaccacac cgtcctgttc catctggtcg
     6001 agacgggaga gcaatttgcg ggtgatttgc tcgagctggt ctttttctgt tgtgctgagc
     6061 gcggaccaga gttgatgcag gcagttatgc tgcggcggta aaacctcgcg caaaaactcg
     6121 tgaccttttt ccgttaattg cagatgcagg cagcggcgat cgttatcgct ttcacgacgt
     6181 tcgatccaac cgcgtttttc cagttcannn nnnnnnngga tgatccaaga gcacaactca
     6241 attcagaagg ctgaatactg tggttttcct gagactccag cgtaatcaac gccataaaca
     6301 acgtctcgtt aatcccctga gccttcagca ttttattgcg gttttccagc agcttgcttt
     6361 gcatatgcat gcaaagacgg gtcagaagga tctcctgata aggaaaatct tcgtggcggc
     6421 tggcgcgaaa ttttagcatt tgttcaatgg gcgtaaacga actatccatt tgggtatgac
     6481 ctcattaatt tcagccgata tagtaacgac agtgacaaat aaagtaaatg tattgtttta
     6541 gaaaaatgat tcttgtgggt tatataatcg ccatcacttt ccaggcgagc ccataggcca
     6601 gcgcactaag cagtgttggg atgataatgc tgcgtgtttt atagaaactg gcacccagta
     6661 ccgcgaagcc gaccagtgtg ggcacgaaac ggcgtgtatc gtgcatcact tctggtgcgg
     6721 tagagacaac cagcagagcg catatcgagg cgatgccaat ggtgtcgagc aaaataccta
     6781 ccgcgccacg tttggttggg cgggcattac ccacacgcag gcgcagcggc agatagcgga
     6841 agcaataatt cgccgcgcca accagtaacc caagcagcag aacctcatag ctcatcgggc
     6901 gctccttgcc agaatgcctg gattaacgca gtgaggcagc cacagacaat gcctgccaga
     6961 atggcgacgg gaatagaaaa tagcgttacg cctgcaaggg caccaactaa cgctgcggta
     7021 acgcaaagag attgtttgcg ctggaaagag gcgagcagga aactcataaa gagtgccgga
     7081 agcataaaac ctaatgctgc ttcaacggcg ggataacctt gcagcaagcc gctgccggag
     7141 aatgccccta ttaccgtacc aaatacccac gatgaccatg aactgaaggc aatgccgatc
     7201 atccagtttt cgctccagcg gcgattattg cgtaccagtt ttgcggttgc ggcggcaaaa
     7261 acctcatccg tcaggccaaa cgcccacagg gcggttttcg atttttgcag acgctgaata
     7321 atacggctac gcagtgacgg gccatacaaa acatggcgaa catccattgc catgacggtc
     7381 agtgcagcaa cccacaaact actcccggct gccagcatcg cggtaatgac gaactggctc
     7441 gcgcctgcat aaatgatgca ggagaaaaaa acgctttcga gaggagagaa tcccagacgg
     7501 gtcgcattca gaccgaacgc aaaggccacc ggaatataac taataacaat cggtaaactg
     7561 tctttgcatc cttccatgaa ggtcgccgaa ccaggagcag gctgtggagt agggctttcc
     7621 atagagtgta cgcttaacga ttgttaatat tataaataga ctgaatgaat atcttaacct
     7681 tatcagactg atgggcttct taacaccctt ataagtgtaa agccacgaaa acggttgctg
     7741 attgcttaat ttgcggcttc ctgacgatga aaacctcgcc accagaccag taagttaacc
     7801 agggcaatcg tcgcgccagc cagacacacg ccagcccaac tgccatgttg ccaggctgag
     7861 gctgaaatta gcgaaccagc ggcaccgcca ataaagtagc tggtcatgta acctgcggtc
     7921 aggcgattac gcgcatcagg gtgtattcga taaattaccg tctggttagt gatatgcacg
     7981 ccctgcacgg tgaggtccag caccaggatg ccgataatca gcgccagtac ggaagtgtga
     8041 ccaaaccaga ttgccagcca tgaaagtaac agcagcagca gaccgaaagt tgtggtgtgg
     8101 tgcgatttgc ccttatcggc aaaaccgccc gccggacgag cgcccaacgc tccggcagct
     8161 cccgcaagcc caaacagacc aattacacca tcgctgtagt taaaaggtgg agcggcaagc
     8221 aaaaaggcca ttgaggtcca gagaatgctg aagttggcaa aggtcaggca gcccagcaac
     8281 gcgcgggtgc gcaggatctt attgctgata aacatgctga aaacggaacc caacaactgt
     8341 gggtagttga ggtgggtttc tgatttcatt tgtggcagac cacgccataa tgccagcgcc
     8401 atcagtgcca ttaacatcga agcaacccaa aagacggtgc gccagccgcc gagattcgcc
     8461 agcaatccgg caacggtccg tgcgcagacc gctcataatg gtgccaacca ctttgccgcg
     8521 tttgtccggt gaagccagcg tcgctgccag tggaaccaga atttgtgcca cgactgaaaa
     8581 taaaccggtt aatgctgtac cgaggatcat catcgccagc gactgactgc tggcagtgat
     8641 taacatgccg catgccgcca gtaaggtcat cgagacaatc aggcggcggc gttcaaacat
     8701 atcaccgagg ggaacaagaa acagcagacc tgctgnnnnn nnnnngctgc atagcccaac
     8761 tgcgcggcgg taacaataaa gcctgccgaa ctggcggaaa gggaaaagtt acgcgcgatg
     8821 gtgtcgagca atggctgggc gtaatagtta ctggctaccg ccaggccggt ggcgatagac
     8881 atcagcacga tcagcgccgg gctaagctca tgattaggtt tagtcattat ttttgttcat
     8941 caggtgatga ttaagctgga aatcaataat agcggatgtt gatgaatatg ggatgaaaga
     9001 tagattgttg aaatgtgcgg tgtggtttgt aggtatgata agacgcacca gcgtcgcatc
     9061 aggcattgtg tacagagcgg cggatgaggc aaaaacgcct tatccgcccg aataaaaatt
     9121 acttctgcgc tgccagcgcc tcattcaccc agccatcgaa ctgctgctgg tgagctttga
     9181 tccagccatc aacgtggccc tgaatatcgc cttctgaggc tttgccgtca tgcataatgg
     9241 cgttctgggc gttaatatca gccactggta actgcataat ggcaaacagt ttcgctgctg
     9301 ccgggttttt ctcggcccag gctttgttgg caacgatatg catggtactg accgggaagc
     9361 cgtaattcgc gccattcggc agtttggtat cggcattttt atcgcccggc agtgcggaga
     9421 acggcacctg caaccagacc acatctttcc ccggtttcag ttcgttactc acccagtacg
     9481 gcgtccaggt gtagtaaaac accggcttac cctctttgta acgactgatg gtgtcagcca
     9541 tcatcgctgc gtagttcccc tgattatgcg tcacggtatt ggtcagtcca tacgcggcaa
     9601 gctggtggtt gatcgcacct tcgcagcccc agcctgggtt acagccggtt aaatccgctt
     9661 taccgtcgcc gttggtatcg aacagtttgg cgatcttcgg atctttaagt tgcgcgatgt
     9721 tggtaatttt atactggtcg gcggttttct tatcgatcag gtaaccctgt gccgcgccgt
     9781 taacaaatac tccttcacga taaaatttct tatcgccacc ggcagcttcg tacatgttgt
     9841 catgcagtgg cgtccagttc actgcggtga aggttgcatc gccggaagca agcgaggtgt
     9901 agccaacgtt gtaatccact tcgctgggtt tattgacggt ataacctaat ttctccagcg
     9961 cacgactgac cagcagtgtc tggaaggttt cttcggtgat ggtgctttga actggattaa
    10021 cagtaatgcc tttgcccggc aggtcggcag caaaagtttg tgtagagata agcgtggcaa
    10081 acgctgtcgc aaaaagtacg ctatgtcgca ttgttattcc tttttttggc agggtgatat
    10141 gcccggcgtc accgggcaag tgcagagtta cttaatgaat gggcgggtca gcagaccgac
    10201 agggccagtg gtgtaccagc gacgattgcc gcgactgcgt gagtcgcgcc caacggcctg
    10261 cgtcaggcgg tcgaggataa tggcgaggat cacaatcccg acgccgccaa cggtggcaag
    10321 ccccatatcc agacgaccga taccgcgaag taccatctga cccagcccgc cgacggcaat
    10381 catcgaggcg atgaccacca tagaaagggc cagcatcagc gtctggttaa cgcccgccat
    10441 aatggttggc atcgccagcg gtaactgaac tttgaacagc atctggcgcg ggctggcacc
    10501 gaatgagcgc gaggcttcaa tcagatccgc cggaacctgg ttaattccca gaatcgtcag
    10561 acggataatc ggcggcagcg caaagatgat cgtcaccacc acgcccggca cgttgccgat
    10621 accaaacagc atgacgattg gcaccagata aacaaacgcg ggcgtggtct gcatggcatc
    10681 aagcagtgga cgaataattt tcgccgctcg cggacttctc gccagccaga tccccaacgg
    10741 cannnnnnnn nngcagggct gttaacacca gcgccagggt aaccattgcc tgtgaccagg
    10801 caccgattgc gccgatggca attagcgaaa ccagcgtcgc cacgcccatt ccgaccccgg
    10861 aaatctgcca ggcgatgaga gcgaaaacga taatcgccac cggcgcgggc atacccagca
    10921 gcaattgctg gaaaccgttg aggatgtaat caaccggaac gcgcacgccc tgaaagacgg
    10981 gacgaaaatg ggtaacgacc cagtcgatcc cttcagtgac ccaactgtcg agcgggatca
    11041 gcgttttatg gaacggatcg agaatattaa aatgctcgac gtttggcgca ggcgtactgg
    11101 tcagccagtc agcaccaccg ccgtcagtcg gtgcagtcgc cggtgtaccc caggcgtctg
    11161 cggat
//
LOCUS       DAWDRC010000050        11105 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3042_length_11105_cov_2.744887, whole genome shotgun sequence.
ACCESSION   DAWDRC010000050 DAWDRC010000000
VERSION     DAWDRC010000050.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11105)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3042_length_11105_cov_2.744887"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2105..2114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3629..3638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4839..4848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5306..5315
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2989 a         2798 c         2528 g         2750 t
ORIGIN      
        1 ctgaaattaa tgccgaaact aaaattaaaa ccttcaaatt acaattcaaa gactgtgcgg
       61 ggattcccgg gaaaaaggcc aaaataaaat tgactacacg agcattatgt gaaggtaatt
      121 ctaataatgg tccaggattt gctaacgcat caacggcaac agcaaaagca gcggcggtcg
      181 ccgttgaggt ctggagcaca tctacgccag gtaaaaacgg cgctaaacaa ttcagttgtg
      241 tcacccccgc aacagcagaa gtcagtattg ctaatgcgac gggcagcaat gttgtcgatt
      301 acccaatgag tgcggtgttg gtggtcgcaa aagataaaac tgtcacaaat gtcactgcgg
      361 gtgatttcac tgccccggca acattcaccg tgacttataa ctaacaggca gactgattca
      421 ggaactaaat atgcgtccca ctcaacgtaa tctgatgaaa aaatcaattc tgattctgtc
      481 gttgctattc agcgccactt cccctgtggt tgcaggacag gatgttgaca ttgttgcaaa
      541 tataaaaaat aacacctgcc aaagtggaat cagcaacaac ggaaatattg atttaggtgt
      601 cgttggcgtg ggcgatttta ccggaaacat ttcagccgaa aattatcacc ccggtggaaa
      661 agagttcact attacagtac aggactgcac acttcagggc acagggaatg tgttaaatca
      721 gttacatatt aattttcgcg cacttagtgg cgtaatggcg acaggttcca gtcaaatatt
      781 cgctaatgaa gacaacgccg gggctaaaaa tgttggggta gttatatttt ctattcagga
      841 cccggcaaat accttcaacg ttcttagtgc cactggtagc tcacgttccg tttatccggt
      901 catgtcatca gcactaaata attcatcctg gaaatttagt acccgaatgc aaaaaatcga
      961 tccagcactg aatgtcacct ctggcccact gataagccat gtgttagtgg atatctacta
     1021 cgaataacag acaaagtgct acaagtatta tcttgcagca tgacaatgga gtaaaaatga
     1081 agacattctt cagatacttt ctttttttag cactatgctc ttgttgttat acagcaagcg
     1141 ccggcaccga tgatgacgtc ggctatattg ttgggaataa ttacggtgtt ggtccatcag
     1201 accaaaaatg gcgagagaca ggtcccaatg gagatgttac cgtcaaattc cggtatggtt
     1261 caggcaccaa taatttagtt ttttataaac caacccaact tggcccaacc ggggtgtcat
     1321 taaaatgggc gcagttagat tccgcttcgg gtggaggttt cctttactgt aacaggagta
     1381 gtaactcaag cggcgcacca atgtctattg aacataaaat ggttgattcc ggaaaaagtt
     1441 atggcggaca taaactcttt aagacatctg ttccaggcct gtattacacg ttagcaatat
     1501 caaacatttg gtcaacactt acatctactg atatcaaccc atcaggcatg tatattggcg
     1561 attctacgag ccaatctttt aactggcgtg gcgaaagcga gcaaacccta tattggtctt
     1621 gcaacaacgc gaatagcagc aaaaaatact gggccgttgg tggcgttatg caaacgctca
     1681 cgattgaatt ttataccgat accgacttta acccgacaac gaatcaacgg gtaacattat
     1741 ccagaacaga tagttatctc tactcattca aagcatataa tgctggcgtt agtatcaaga
     1801 gttattttct taaaatcgac tttgacttga cagatatcgt attaacaaac cctacctgtt
     1861 ttactgctgc gcttagtggc ccatcggtaa gtggttcaac ggttaaaatg ggtgattata
     1921 gccctgcaca aataaaaaat ggcgcaacag cggttccttt tgatatcacg ctacagaact
     1981 gtattcgtgt tcgcaatata gaaacaaaac tcaagtcaaa caaggtgggt agtgtaagca
     2041 aagaactttt ggctaacaca ctcacaggaa atgacgcagc aaaaggcgtt ggtgtactga
     2101 ttgannnnnn nnnncaaatg atgcaacgtc tatttataaa gattatgaaa cagaaaatga
     2161 cactactggg gggattttcc cggataatgg caacggtggg acctctcaac ctcttcattt
     2221 ccaggctaca ttaaaacaag acggtaatat tgctatagaa ccgggggatt ttaaagccac
     2281 cagcactttt caggttacct acccctgata acactattgc accgccagac aacggcggtg
     2341 cgatacggat aaatcattgc ccccaccaat gtgactgata aaatgcgaga cagcacaaca
     2401 tcatttatca ggatacgcta tgaaaccaac cacgattgct tcattgcaga aatgcaagca
     2461 ggacaaaaaa cgtttcgcaa ccatcaccgc ttacgactat agcttcgcaa aactgtttgc
     2521 cgaagaagga cttaacgtca tgctggtcgg cgattcactg ggcatgacgg ttcaggggca
     2581 tgactccacg ctaccggtta ccgttgctga tatcgcctat cacaccgccg ctgtgcgccg
     2641 cggtgcgcca aactgccttc tgttagcaga cctgccattt atggcgtatg ccacgccgga
     2701 acaagccttt gaaaacgccg caacggtaat gcgtgccgga gccaacatgg tcaaaattga
     2761 aggcggtgag tggctggtgg aaaccgtaca aatgctgacc gaacgtgccg ttccagtttg
     2821 tggtcactta ggtttaacgc cgcagtcagt gaatattttc ggtggctaca aagtacaggg
     2881 gcgcggcgat gaagcgggcg atcaactgct cagcgatgca ttagccttag aagccgcggg
     2941 ggcacagctg ctggtgctgg aatgcgtgcc ggttgaactg gcaaaacgta ttaccgaagc
     3001 actggcgatc ccggttattg gcattggcgc aggcaacgtc actgacgggc aaatcctcgt
     3061 gatgcacgac gcgttcggta ttaccggcgg ccatatccct aaatttgcaa aaaatttcct
     3121 tgctgaaacg ggcgacatcc gcgcggctgt gcggcagtat atggctgaag tggagtccgg
     3181 cgtttacccg ggcgaagaac acagtttcca ttaaggagtg acgttgtgtt aattatcgaa
     3241 accctgccgc tgctgcgtca gcaaatccgc cgcctgcgta tggaaggtaa gcgcgtggcg
     3301 ctggtgccca ccatgggtaa cctgcacgac ggccatatga agctggtcga cgaagccaaa
     3361 gcccgcgccg atgtggtcgt cgtcagtatt ttcgttaacc caatgcagtt tgaccgcccg
     3421 gaagatctgg cacgttatcc acggaccttg caagaggact gcgaaaagct gaacaaacgt
     3481 aaagtggatt tagttttcgc gccttcggta aaagagatct acccgaacgg tactgaaacc
     3541 cacacctatg ttgatgttcc cggtctttcc accatgctgg aaggtgccag ccgtccggga
     3601 cattttcgcg gcgtttcgac tattgtcann nnnnnnnnca agctgttcaa cctggtccag
     3661 ccagatatcg cttgcttcgg tgaaaaagat tttcagcaac tggcgctgat ccgcaaaatg
     3721 gttgctgata tgggcttcga tattgagatt gtcggcgtac cgattatgcg tgcgaaagac
     3781 ggtctggcgc tgagttcccg taacggttat ctgacggcgg aacaacgtaa aattgcgccg
     3841 ggtctctaca aagtcttaag ttcaattgcc gacaaattgc aggctggcga acgggatctc
     3901 gatgaaatta ttgctattgc cgggcaagaa ctgaatgaaa aaggcttccg ttccgatgat
     3961 attcagattc gcgatgccga caccttgctg gaaatctctg aaaacagcaa acgcgcagtg
     4021 atcctggtag ccgcctggct tggcgatgct cgcctgatcg acaacaaaat cgtagaactg
     4081 gcgtaatatt taactggcgc tacggctgat ggcgccagtt attcattgcc acgtcatctg
     4141 taagcaatta ttcatatcgt agacaactta gttagagtta attagcttat caatctctgc
     4201 aagagataaa gctgtcacct ctgcaacgtc ttcccgagac attcctttac tcagaagacg
     4261 ctgggccagt cttaccttct ggagtccctc tttatatccc ttctgaaatc ccttctgaaa
     4321 tcccttctct tcaaaccact gcgccaacgt ctgccaggtt tctctccgtg tctttatttc
     4381 catatgttcc cttaattatc tggtcagtta acaataacaa ttatctggct ataactgata
     4441 atgcagataa cttagctaat cagcttatca acctcagcaa gaggtaaatt tgccatctct
     4501 gcaacgtctt cccgagacat ccctttactc agaaaacgca gggcgtattc ctgtcttact
     4561 tcctgtctaa cctcttgtat tacctcctgt cttacctcct gtcttacctc ctgcctttcc
     4621 tcctgtcttc ccttctcttc aaaccactgc gccagcgtca tcatagactc ccctcccgtt
     4681 tccctgtctc tcagcacacc ataaaacaaa tccgcttgtt cagtatgacc gcgttgcagc
     4741 atatagtttt gcatggcaac taactgactt ccgctagtgt acccttcgtc aatcagcgtg
     4801 accagttgct ccagcaataa cattaagtcg cgctggcgnn nnnnnnnntg tttttgcagc
     4861 agttcgggaa tcgcaatccg ccgatgttgc atgatttcgt catccggtgt gatagtgata
     4921 tccaccagcg ggaaaggact gttatagacg cgtcgcgcca gttccggtga gtaaaacata
     4981 tcaaaccagc acattgatag cggataaggt gtggcctcgc cctgataaaa cagaattggc
     5041 accaccagcg gcagcttatc gtggtcagcc tccagatgcc ggtgcatggc agcaatagaa
     5101 taacgcatca tgcgaaagac cattttctta tccggcttgc tttgatgttc aatcacaaca
     5161 tgcaaataac cggggttgcc ctgcatttgc acggaataga gcacgtcagt gctgtgccct
     5221 ttcaggcttt cttcaataaa actccccgac tcaagatgca gcgtgtcgag gtcacacagt
     5281 tcgcgtagct ccgctggcaa atggannnnn nnnnntcgcg agccgtttcc gcatgcatta
     5341 aaaattgttt aaacaccgca tcatgcggtg tggtactcgg tgcatccatc ttccctgcct
     5401 tccgtgtggt cttgcgtgaa ggcagtatcc agtcatggtg tgccgtcgtc agccgcctct
     5461 ttagtgttta ccgagcagcg ttcagataaa tttcaattgg ttacaggcgt tacatggcac
     5521 gcttcggcac ggtacgcaaa ctgctgctgc aaatgccgtc agatgagtag acactaaaca
     5581 aaaatcgggc aatactgcgt gagaatttcc tcaaagcagg catcgcctgc ttcgttaacg
     5641 acagggtaga aaggtagaag ttatgattcg cacgatgctg cagggcaaac tccaccgcgt
     5701 gaaagtgact catgcggacc tgcactatga aggttcttgc gccattgatc aggattttct
     5761 tgacgcagcc ggtattctcg aaaacgaagc cattgatatc tggaatgtca ccaacggcaa
     5821 gcgtttctcc acctatgcca tcgcggcaga acgcggttcg agaattattt ctgttaacgg
     5881 tgcggcggcc cactgcgcca gcgtcggcga tattgtcatc atcgccagct tcgttaccat
     5941 gccagatgaa gaagctcgca cctggcgacc caacgtcgcc tattttgaag gcgacaacga
     6001 aatgaaacgt accgcgaaag cgattccggt acaggttgcc tgattgttat ccctgcggct
     6061 ggttactcac cagccgcgac atcgtctcca gcgaatccgt tcttaagata taaagtcgtt
     6121 ttagtaacaa cggattatcc cccggtttta ctttgccttt catggtcgtc accgccaggt
     6181 gaaatccggc ttcttttgct gccttcacgg ctttgtcatt aaatccgcca aatggataag
     6241 aaagatacaa aacatgtgga ttaaattgcg ccagagcgcg gcgtgaacgt gcaaaatcaa
     6301 acagaatatt gtgctcacta cggctcagca atataggccg ccgataacca tctatccgat
     6361 gcaaaaaatg agtatgtgac tggaaatcaa atacatcgcg aatttcgtta agttcagaaa
     6421 cgctcataaa ttgcagcgat tttgggttcc atttctgcgg gtgacgtttg atgcgcgagg
     6481 taacaataaa cgccgtcgcc ttcatgccat attgtttcaa cacaggatac gcatagcggc
     6541 tcaccgactt gaggccatca tcaaaggtaa ttaccaccgc tcgcgcaggg agattgatct
     6601 tattcttcac gtaaccttcc agctgcgcca tgctcagtgt cgcgtatccc ctgtcgcgca
     6661 gccaggccat ctggttattg aaagcgcgta ccgaggtggt cgtcgaagta tggcgaaaac
     6721 gggtgttttc ttcgtcgcgc aaaatatggt gataggttag caccggcaag ccattatcgg
     6781 gttgggcatc cagcgcgctg atataggcca gtcgatcgcc aatgcggatc tgataccagg
     6841 tttgatttaa cctgtctttc agtttatgca aaatcgggta gcgcaaattg tccgccagta
     6901 ctccaaatgg cgcactgccc acactcggcg cgttataaac cggcgtatct ttccaggtta
     6961 ttaagttctg attactcagc ggcttgttga ggtcacctaa accatcttca actttttgtc
     7021 gcccctgaac cggctcgaga tggcctttat cgataaaccc tttaccaaag ccaaaattaa
     7081 atacgtaata acttgcggca gtgggctcca cggcaatgat ttgtccggcc cgaatattcc
     7141 ccaccgtcac catcttgtca ccaatttgcg cccagaccgc ggcattttcg atggtttgca
     7201 tataatgggc aggtaatgcg gcactgacgc ttgcggtaaa cagcatcagc aggagaataa
     7261 cagcttgttt gtacatgaaa aaataaccga gggtgaaaca acgcttttat tgtagcaaaa
     7321 gcgtcatcag aatcgcgggt taatactcat acaaattatg tttgaggacg aaaggcggat
     7381 ttttttgttg ttggtgccaa agactactca cctccggcgc acccagttcg acaatatgct
     7441 cgcgaaacgc tgaactggtc atggtttcac ggcaagccat catctgttca attaacggta
     7501 acgtgacacc agaaatcact tcgcaacggg agtgtttgtg gcttaataat gaagccacgc
     7561 ggtacggtgg cgctccggct atatcggtta aaaagataac gccctcacca gagtccgttt
     7621 catgcaaggc atcgcacatc atgcggctga gcatattaga ggttaatccg cgccagaaat
     7681 tcacggcccg gcactgaaga agtgccccat gttttttctc cagcgcatcc agtatctctt
     7741 gcgcccggtc atcgtgacag gtaattaccc aacctaacat cgccttcctc cttagccgaa
     7801 aatcagtcta ccctgaccat tgttgataaa gggtgatagc cgtcaaaact cctctccccc
     7861 aaatccagag gagaggaaat agccttagct acgcaaacca cgtccacgtt ggatcagcga
     7921 ccaacagatc aaataaaacg ccacaataaa gaccaccagt acgccaaagg tagtgaccag
     7981 cggaacatca ttgataccga ggaagccgta gcggaaacca ctgatcatat aaacgattgg
     8041 gttcaggtgc gacagccctt gccagaacgg cggcaacaaa gtcagggagt aaaacacccc
     8101 gcccaaatac gtgagtggcg ttaacacaaa ggttggcacc aggctgatgt catcgaacgt
     8161 tttggcaaac acgccgttca gcaaacccgc aagggagaat agcaccgcag tgagcaccag
     8221 ggttaaggca acgaataccc acgaatgcac ctgaaacggc acaaaaaaca gtgaaattgc
     8281 cgtcaccaaa ataccaacaa acagaccacg cgccacgcca ccaccgacat atccggcaat
     8341 aatgacatga gtcggaaccg gcgctaccag cagctcttca atattacgct ggaacttggc
     8401 accaaaaaat gatgacgcga cgttagcgta ggcgttggtg atcaccgaca tcatgatcag
     8461 ccccggtacg atgaactgca tgtagctgaa gccatgcata tcgccaatac gcgaaccaat
     8521 caggttaccg aagataataa agtaaagggt catggtgatg actggcggca ccagcgtctg
     8581 cacccagata cgcataaagc gatggatctc tttcgcccag atgcttttta gcgccaccca
     8641 gtaaagatgc atcatgtgcg atctccttgt ttttcattaa ccagtgaaac aaacagctct
     8701 tccagacggt tagctttgtt acgcatactt aatacctgaa tgccctgctc acttaactgc
     8761 gtaaatacgc tgttgatgcc ctgctcacgc agcacttcaa cttccagcgt ggcggtatct
     8821 accagtcgat actgatagcc atcgagcttc ggtaacggac ttttcggtgc gagatcgaga
     8881 ataaaggttt ctgatttcag cttcgccagc agcgccttca tcgaagtatt ttccaccaac
     8941 tcaccgtgtt gaataatgcc gatattgcgg cacagcattt ctgcttcttc caggtagtgt
     9001 gtggtgagaa tgatggtggt gcctttgtcg tttaaatcct tcaaaaagcc ccacattgag
     9061 cggcgaagtt caatatccac gcctgcggtc ggttcgtcga gaatcagtag tttaggttca
     9121 tgcattaacg cacgggcaat cattaaacgg cgcttcatcc cgccagataa catacgcgca
     9181 cgttcgttgc gttttcccca tagatcgagt tgtttaagat acttttcgct gcgaatgtac
     9241 gcttctttgc gctccacacc gtagtaccct gcctgattca ccacaatttg ctgcacggtt
     9301 tcaaacgggt tgaagttaaa ttcctgtggc accagaccca actgacgttt agcgtttacg
     9361 acatctttct cgagatcgta accaaatacg ctgacccgcc cggaggtttt atttaccaga
     9421 gagctgataa taccgatagt ggtcgatttt ccggccccgt tcggcccgag aagcgcataa
     9481 aaatcacccg cttcgacctg caaatcgatt ccacgaagcg cctgaacgcc gcctggatag
     9541 gtttttttaa gctgttgaag ttccagtgca atggtcataa atttttactt accttacgtt
     9601 cttacacttt atctgtggtt taaatcgtcc cggagttgcc ctatattagc caaacgtaat
     9661 tatttggtta caggtcgtta acctccatga aagacataga tacactcatc agcaacaatg
     9721 cactatggtc aaaaatgctg gtggaagagg atcccgggtt ttttgagaaa ctggcacaag
     9781 cgcaaaaacc gcgctttcta tggattggat gttccgacag tcgcgttcct gcagaacgtt
     9841 taaccggtct tgagccgggc gaactctttg ttcaccgtaa tgttgctaac ctggtcattc
     9901 acaccgacct gaactgcctt tccgtggttc agtatgcagt ggatgtactc gaagttgaac
     9961 acattattat ctgtggccac tacggttgcg gcggcgtaca agccgcagtt gaaaacccgg
    10021 aactggggct tatcaacaac tggctgctgc atatccgcga tatctggttc aaacatagct
    10081 cattgctcgg cgaaatgccg caagagcgcc gtctggatac cttgtgtgaa ctgaacgtca
    10141 tggaacaggt gtataacctg ggccactcca ccattatgca atcagcgtgg aaacgcgggc
    10201 agaaagttac cattcacggc tgggcctacg gcattcacga cggcttgctg cgtgatctgg
    10261 atgtcacagc gactaaccgc gaaacccttg agcaacgtta ccgtcacggg atctccaacc
    10321 tcaagctgaa gcacatcaat cacaaataaa ttcgccatgc cggatgcaac acatccggca
    10381 acttcacact tactcgtcca gcagaatcac tttgccgata tacggcagat gacggtaacg
    10441 ctgtgcgtaa tcaatgccgt aacccaccac aaactcatcc gggatcgaga aaccgataaa
    10501 ttctaccggg acgttcactt cacgacggga cggtttatcc agcagcgtac aaatcgccaa
    10561 cgacttcggt tcgcgcaggc ttaagatctc acgcactttc gacagtgtat tccccgagtc
    10621 gatgatatct tcaacaatca gcacgtcctt gccacggata tcttcatcca gatctttgag
    10681 gattttcaca tcacgggtgg tggacatgcc gctaccgtag ctggaggcgg tcataaagtc
    10741 gacttcatga gatacctgaa cttcacggca caggtccgcc ataaacataa atgagccacg
    10801 cagcagaccc accagcacca tatcgctgcc gctgtctttg taacgctcag taatctgacg
    10861 acccagttcg gcgatacgcg ctttaatctc cgcttcgggg atcattactt ctacagtatg
    10921 tttcatatct ataaccatat gatttaaaaa caaatcactt cgcacgggca ggtttcagca
    10981 ccgcaacaaa gcgcaagaca cgcagtatac cagtaaaact attcattcgc ggaatctgtt
    11041 aataaagccc gaattgtgat gacgatcaca catgttaaaa cctataatta attgctacta
    11101 aaata
//
LOCUS       DAWDRC010000051        11024 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3064_length_11024_cov_1.984866, whole genome shotgun sequence.
ACCESSION   DAWDRC010000051 DAWDRC010000000
VERSION     DAWDRC010000051.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_11024_cov_1.984866"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3460..3469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6470..6479
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6785..6794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7440..7449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9268..9277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    10434..10443
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2697 a         2920 c         2705 g         2642 t
ORIGIN      
        1 ggcgaaagat ctcggtgcca gtgaatttat cattctgcga aaaatcatcc tgccactggc
       61 aatgccagcg gtggcggcgg gctgggtgct aagctttacc ctgtcgatgg acgatgtggt
      121 agtttcgtcg tttgtcaccg gaccaagcta tgaaattctg ccgttaaaaa tttattcgat
      181 ggtcaaagtc ggcgtatcgc cggaagttaa cgcgctggca accatattac tggtgctgtc
      241 gctggtgatg gttattgcca gccagcttat tgctcgtgat aaaacgaaag gtaacacagg
      301 ggacgttaaa tgaaaaaatg gtcacgccac ctgctcgcgg cgggtgctct ggcactgggc
      361 atgagcgccg ctcacgccga tgacaacaac acgctgtatt tctacaactg gaccgagtac
      421 gtgccgccag gactgcttga acagttcacc aaagaaaccg gtattaaggt tatctattcg
      481 acttacgagt cgaacgaaac catgtacgcg aagctgaaaa cgtacaaaga cggtgcctat
      541 gacctggtgg ttccttcaac ctattacgtc gataaaatgc gtaaagaagg gatgatccag
      601 aagatcgaca agtcgaagtt aagcaatttc agcaacctcg atccagacat gctcaacaag
      661 ccgtttgacc cgaataacga ctattccatt ccgtatatct ggggtgcaac ggcgattggc
      721 gttaacggtg atgcggtgga tccgaaatct gtcaccagct gggccgatct gtggaagcct
      781 gagtacaaag gcagcctgct gctgaccgac gatgcccgtg aagtgttcca gatggcgctg
      841 cgtaagctgg gctactccgg taacaccacc gatccgaaag agattgaagc tgcatacaac
      901 gagctgaaaa aactgatgcc aaacgtcgcg gcgtttaact ccgataaccc cgctaacccg
      961 tatatggaag gcgaagttaa cctcggcatg atctggaacg gttctgcttt cgttgcacgc
     1021 caggcgggta cgccaattga tgtggtgtgg ccgaaagaag gcggcatttt ctggatggac
     1081 agcctggcga tcccggcaaa tgccaaaaac aaagaaggcg cactgaaatt gatcaacttc
     1141 ctgctgcgcc cggatgtggc aaaacaggtt gctgaaacta tcggttatcc aacgccaaac
     1201 cttgcggcgc gtaagctgtt aagtccggaa gtggcgaacg ataaaacgct ctacccggat
     1261 gctgaaacca ttaaaaatgg tgaatggcag aatgacgtag gctccgccag cagcatttat
     1321 gaagagtatt atcagaagct gaaagcagga cgttaattca gcaagcgggc ggtggtatta
     1381 ccgcccgttt tttatttagc aaggatgtgg ctaatgagcc aggacagtag agttttcttt
     1441 cgtatattct tcggtatcgg tctggtactg atcctcattt ctgttgttat cttttataac
     1501 cagttcacct atagcaaaga cgcgattcat actgaaggcg tcattgttga taccgtctgg
     1561 cactccagtc attctcatcg cacgggtaaa aatggttcgt ggtatcccgt cgttgccttt
     1621 cgtccgacgc cagattacac gctaattttc aattcgagta ttggcagcga tttttatgaa
     1681 gacagcgaag gcgataaagt taatgtttat tattctccag gacatcctga aaaggctgag
     1741 ataaataacc tgtgggttaa tttttttaaa tgggggttta tcggcattat gggcgttatt
     1801 tttatcgccg tcggtttatt aatcagtatg ccctcctcta aaaagacacg caggaaacgg
     1861 aagtcccgcc catgaaaaaa atcatgatac tgctcagttt actgatgttc ctgcctctga
     1921 cggcaatcag taagccctta attccaataa tgaagacact ttttacggat gttaccggta
     1981 ctgtaccaga tgctgaagag atcgcgcata aagccgaact ttttcgccaa caaaccggag
     2041 ttgcaccgtt tattgtggtt ttacccgaca ttaataatga agccagtctc aggcaaaatg
     2101 gcaaagcgat gctggcgcat gcggcatctt caatgagtaa tgtaaaagga agtgttctgt
     2161 tactctttac tacccgcgag ccacggttaa ttatgataac caacggccag gtcgaaagca
     2221 gtatggacga taaacatctc ggccttctgg tagaaaatca cacgctggct tatttacatg
     2281 cggatctctg gtatcagggt atcaataatg cattggctgt tttacaagca cagatattaa
     2341 aacaaccgac gccaccactg acatattatc cgcatcccgg acaaccgcat gaaaatgatc
     2401 cacccggcag taccacgaca ttaggccttt ttgcctgggc agttgcattc atcgtctttg
     2461 ctgcattttt taactacaca acgcgccttt attatgcgtt aaaatttgca gtggcgatgg
     2521 ctgttgccaa tatgggttat caggctttat gtttgtatat cgataacagc tttgcgctta
     2581 ccagaatatc gccactctgg gcagggctga ttggtgtctg tacgtttatt gccgcgttac
     2641 tatggacgag taaacggtaa aagaggcagt aaagttcgca tgtattatgc cggatgcggc
     2701 gcgaacgcct tatccggccc acaaaacccg caaattcaat taattgcgcc ccttcacagg
     2761 cctgacaagc gtagcgcatc aggcaatttt tcccgcttta taatcccttc agcaactttt
     2821 caacaaactc tggcaccacc tggcttgccg gaccgtaata tttctcggca aattcattac
     2881 caacctgact cggttcaaga ttcagttcca cggtgtgcgc gccatgcagt ttcgcttcgt
     2941 gaacaaaccc agccgccgga taaacatgcc ccgaagtacc aatggcaatg aaaatatcgg
     3001 ccatcgacaa cgccatataa atttcatcca tgccgagtgg catttcgcca aaccacacta
     3061 cgtgcgggcg caagggtgcc gggaactggc aacaatggca tttatcttct ggcgtaacat
     3121 ctccggtcca gtcgaggacc tggccacttt gcgaacaacg tactttcagc agttccccat
     3181 gcatatgaat gacattggta ttacctgcgc gttcatgcag attgtcgata ttctgcgtca
     3241 ccagcaaaaa gcgatcgccg agagcatctt gcagtttcgc cagcgcaaga tgtgcagcgt
     3301 taggctgaat ttctggctgc tgcaactgtc gacgacgggc gttataaaac gtttgcacca
     3361 gttcaggatc gcgatcaaaa ccttccggcg ttgccacatc ttcaacccga tgttcttccc
     3421 acaggccatc tgcggcgcga aaggtacgaa tacctgattn nnnnnnnnnc tgattccgca
     3481 gaaatcccag cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact
     3541 ttatctctga aaaaaatacg ctgacgcaaa cgctcgcgca ggcggcgttt atttttacga
     3601 aaacgactta accgatgacc ccgacgcgac agcatagcaa cctctgttga ttaatcggtt
     3661 agatgtagga aggccgcacc acgcattccc cccgcatcac cgtggcgcgc gcgttcaatg
     3721 cgcggaacac gagctacagg taagagatga cgaggcaacc tttccgccag ttgcgttgtg
     3781 attgccggga aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc
     3841 aggatatttc ccagacaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc
     3901 tgctcatcgc cttgatcata aagcgcaatg atctcaggag cctgcaacgg ttgatgataa
     3961 tagtgttgat acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc
     4021 tgaccacagc cgcagcggcg taacgggaaa tccagcccca tcatggttaa cgcatcaacc
     4081 ggcagacgca tatggccaaa ctcgccggta atatagcttt taccggtaat tggtttaccg
     4141 ttgaaaatca gcccgccgcc aacgccggtg ccgagaatca accccatcac cagtggatat
     4201 tgagtaaatt catcatccca tgcttcagaa agggcaaagc agttggcatc gttatcaagg
     4261 cgtacatcgc gatcaagacg tgcgctcagg tcggcacgca gcggtttacc gctggcagca
     4321 gggacattgg cggcatacag cgtaccatct tctgtttccg gcattcccgg aataccgatg
     4381 ccgacagaac ctttacagcc aaaacgccgg tcagcttcgg ctaccagctc gcacactgca
     4441 tctaaaaatg cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc
     4501 cgaccgctat caaacacgcc tagcgcaatt tttgttccac caatatcaaa cccgtaatac
     4561 attgccgctc cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc
     4621 aatattactg gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt
     4681 gaccagtacg tagaagacgt ccagccagtg caattccgat ggcaagaagt caataaaata
     4741 gatatcgctg gagaggaact gatgaccgat cagcttttca atccactcaa taatcggggt
     4801 aagttgcagt gaaacaacta cgccgataat cacaccacac aggctgccga acagccctgc
     4861 cagcaatcca taccagacaa agatggcgcg aattaagcca tctttcgccc ccagcgttct
     4921 taatactgcg atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt
     4981 gaaacaggcc acaccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg
     5041 gatatcgcga tacatatagc cgtaagtacc aatccagctt ttaatataaa catagctgtt
     5101 ggtcacttcc cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag
     5161 ggcaatacct gacacgctgg aacccatatc aagatattgt tgggcatccg ccagtgggat
     5221 catggcaaaa ctgtgatcga gttgaccact caactgcaaa ataccggcaa cgtgcaaacg
     5281 cacacgtttt ggctgcatca gtttatgctc aggattcgag ttggggatca taatcgacac
     5341 ccaatcgccc tgcttcactt tcagcgcatt cgccacgcct ttgccgatga taatttgctg
     5401 ttcgcccgct ttaaaattgc gccaggcatc cccctgaaca aacgagggta atgcgctcag
     5461 acgctgttcc tgttgcgggt taacgccctt cacctggatt gcgcgcagat tcgctccact
     5521 ttccaccagc ccggtgaaat tgatatacgg cgcagcggcg gcaatacccg gcactttctg
     5581 cactttatcc agtgcttcct gccagttagt ccacggctga tccaccgctt cgatttcacc
     5641 atgcggcacc accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct
     5701 taagccgacg atcaataccg ccacgccgag ggcaatgccg atggtagaaa tgacggagat
     5761 cagcgacacc atgccgccgc gccgccgtcc gcgactaaaa cgcaggccaa ttaataacga
     5821 taaaggcatc gccattactc cgcccccatc aggctcagtt ccgccgtcag acgaccatca
     5881 cgcatctcca gttggcggct catacgtttc gccagttgca ggtcgtgagt aaccaccagg
     5941 aaggcggtgc cctgcaagcg attcaattcc ccaagcaact ggaagatgct gtcggcgtta
     6001 cgcgcatcga ggttaccggt aggttcatcc gccagtacca gacgcgggtt attgaccagc
     6061 gcacgggcaa tcgccacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc
     6121 gcacgatgct ccagccccac cgcttttaac atctccagcg cacggctgtt gatttcagcg
     6181 ggctttttct tgccaatcag cagtggcata gccacgtttt ccagggcagt aaaatccggc
     6241 agcaggtggt gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc
     6301 gccgcagaca gtttgctcat cggttgaccg ttaaagatca catcgccgga ggttggtgta
     6361 tccagcccgc ccagcaggtg cagcaaggta cttttaccgg aaccagagct accgacgatc
     6421 gccatcattt caccttcgcc gacgctgaaa ctgacattgt gcagtacatn nnnnnnnnnt
     6481 cggtttgcac actgccttcc tgatagcgtt tgcacaggtt gtcgcattgc aacaggatct
     6541 tattcataac gtaaagcctc agcgggttga gtggcggcag cgcgccatga agggtaaagc
     6601 gtagacagca gcgcgatagc catcgccacc agcgcaataa caatgacctg taaaggttcg
     6661 atagcgaccg gcagcgccgc gccatcaagc aggacgccga ttatcggcat cagattattt
     6721 aactggctgg caagcagtgc gccaagcgcc gctccgagga tcgcaccgat aatcccagcg
     6781 ctggnnnnnn nnnntggccc cttgcaccat aaagaccatc atgatttgtc gcggagttaa
     6841 gccttgcgtt tgcaggatcg cgacttcgcc ctgcttctcc atcaccatca gccctagcga
     6901 ggtaataatg ttaaacgccg caacggcgac aatcaggctc agcagcaagc ccatcatatt
     6961 tttttccatg cgtacggcct ggaacagctc gcctttacgg tcacgccagt cctgccattt
     7021 gctgccttca ggcagttttt gctgacttaa tgagtcgact ttcagcggct catccagcca
     7081 caaacgccag ccggtaatat tgcctgccgg ataacgcatc agacgcgagg catcctcaat
     7141 attcaccagc atttcatagc catcgacttc actgttggcg gcgaaagtgc caatcacatt
     7201 gaacaggcgc tggcttggaa tacgccccat cggcgtgaac tggctggcag atggcaccat
     7261 cacgcggatt tgatcaccgc gattgacgcc caactgtgag gcaagctgtt caccgaggat
     7321 gacattatat ttccccggct cgaggtcagt ttgtttcaca tcgaccagat acggcgttag
     7381 tggatctttt tgcgccgggt cgatacccag catcaccccg accgccacgc tgcgcgcgcn
     7441 nnnnnnnnnt accacatcac cggtagtaat aggtgcgacg cgattaacgc cgtccagttt
     7501 gactaccgtt tccgggagtt gctgcgggtt aagagagcca tgctcagaag agagaattgc
     7561 ctgtggcatc aggccaagga tgttgttttg cagttcgcgc tcaaagccgt tcatcactga
     7621 caatactgtg accagcgcca tcaccccgag ggtaatgccg atggtagaaa gccaggagac
     7681 gaaacgaccg aagcgatccg ctgcacgccc acgcatgtaa cgcaggccaa taaatagagc
     7741 gacaggttgg tacatgaaat ccgtctggtt gctgtagcaa agacacgagt atataagcga
     7801 aagcgggatg ggtaaacgtc tgaactgtaa gtgatcgtca gggctaatct gaaaagtacc
     7861 agcaaatcaa tcgactaaag ggcctcattt tctcattggc cttgtggcct gatctttatc
     7921 tgaaaaataa ccaaatacag acagatacgc attacaccac gcgactggcc cctcaggatc
     7981 aacagcatgt taaataaaaa gagcgtgttg tcctgatgaa acaaaaagag ctatggatta
     8041 accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc attacctttt
     8101 atccgcatct caccactttt cagcatccgt tatcggaagt cctgagcaaa tgctggatct
     8161 atttcaatct ttaccttgcg ccctttcgta tgccggtttt tttctttatc tctggctatt
     8221 taattcgccg ctatatcgac agcgtgccat ggggaaattg tcttgataaa cgcatctgga
     8281 gcatcttctg ggtgctggca ctttggggcg tagtgcagtg gctggcgata agtgcactaa
     8341 atcagtggct ggcacctgag cgcaatttaa gtaatgcctc caatgccgct tatgccgatt
     8401 ctaccggtga gttcctgcac gggatgatca ccgccagcac cagcttgtgg tatctgtatg
     8461 ctttaattgt ctatttcgtg atatgtaaaa tttttaaccg cctggccctg ccgttatttg
     8521 ttctgtttgt actgatgagt gtggctgtta atttcgtacc cacaccgtgg tggggaatga
     8581 acagtgtgat ccgcaatttg ccttattaca gccttggcgc atggtttggc gcaacattaa
     8641 tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct ttgctggctg
     8701 ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg gtatcgattg
     8761 tggtgatcat gaagctgttt tatcattacg agcagcgttt cggtatgcgt tcctccagcc
     8821 tgctgaatgt gattggctcc aacaccattg ctatctatac cacccatcgc attctggttg
     8881 aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg ccacaagtcg
     8941 aactgacact cctgctggtt tacccctttg ttagtttgtt catctgtact gttgcgggct
     9001 tgctggtaag aaaactttca cagcgcgcat tcagcgatct gttgttctcc ccgccttctc
     9061 tgcccgcggc cgtcagttac tcccgctaag ccatcgcccc agccggggcg attcaatttg
     9121 ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat gcccccatat
     9181 gttgaggcgt atcctaacga gaatctgaca accgttatgc ctgaacaata tcgttatacg
     9241 ctgcccgtca aagcgggtga gcagagtnnn nnnnnnntgc ccgtatgtgc gggtgtcctg
     9301 cgtctgctgg gcgagttaac cggcgctgcc tgtgcgacgc tggtagcgga aattgccgaa
     9361 cgtcacaccg gaccggtggt gcttattgcg ccagatatgc aaaatgctct gcgtttgcat
     9421 gatgaaatca gccagtttac cgatcagatg gtgatgaatc tggcggactg ggaaaccctc
     9481 ccttacgaca gtttttcgcc tcatcaggac attatctcct cgcgcctttc caccctttac
     9541 cagctaccga ccatacagcg tggcgtactg attgtgccgg tgaatacgct tatgcagcgc
     9601 gtttgcccgc acagttttct ccacggtcat gcgctggtga tgaaaaaagg tcagcggtta
     9661 tctcgcgatg cattgcgaac ccaactggac agcgcaggtt atcgccatgt tgaccaggta
     9721 atggagcacg gcgaatatgc cacgcgcggc gcgttgctgg atctcttccc gatgggcagt
     9781 gagctgcctt atcgtcttga tttctttgat gatgaaatcg acagcctgcg ggtgtttgac
     9841 gtcgatagcc agcgcacgct ggaggaagta gaagagatca atctgcttcc cgcgcacgaa
     9901 tttccgaccg ataaagcggc aattgaactg ttccgcagcc agtggcgtga taccttcgaa
     9961 gtgaagcgcg acccggaaca tatttatcag caagtgagta aaggcacatt acctgccggg
    10021 atcgagtact ggcagccgct attcttcagc gaaccgctgc cgccactgtt cagttatttc
    10081 cctgccaata cgctgttggt gaatactggc gatctggaaa acagtgccga acgtttccag
    10141 gctgacacgc tggcgcgttt tgaaaatcgc ggcgtcgatc cgatgcgtcc actgttacca
    10201 ccacaatcgc tctggctgcg ggtagacgag ctcttttcag agctgaaaaa ctggccgcgt
    10261 gtgcagctaa aaactgaaca tttaccgaca aaagcagcga atgccaattt aggtttccag
    10321 aaactgccag acctggccat tcaggcacaa caaaaagcgc cgctggatgc gctgcgtaag
    10381 ttcctcgagt ctttcgacgg tccggtggtg ttctcggtag aaagtgaagg ccgnnnnnnn
    10441 nnnatgcgta acctggcgct gatttgcgaa agcgatctgc tcggtgaacg cgttgcccgt
    10501 cgtcgtcagg attctcgccg cgccatcaac cccgatacac tgatccgtaa ccttgcggag
    10561 ctgcatattg gtcagccggt ggtccatctg gagcacggcg tcggtcgtta tgccggaatg
    10621 accacgctcg aagctggagg cattactggc gagtatttga tgctcaccta tgccaacgac
    10681 gccaaactgt atgttccggt gtcgtcactg catctgatta gccgttacgc gggtggcgca
    10741 gaagaaaacg caccactgca taaacttggt ggcgatgcct ggtcacgcgc acggcagaaa
    10801 gcggcggaaa aagtgcgtga tgtggcggcg gaattgctgg atatctacgc gcaacgcgcc
    10861 gccaaagagg gcttcgcgtt taaacacgat cgtgagcagt atcagttatt ctgcgacagc
    10921 ttcccgtttg aaaccacgcc ggatcaggct caagccatta atgcggtgct tagcgacatg
    10981 tgtcagccgc tggcaatgga acgtccggtt tgcggccatg ttgg
//
LOCUS       DAWDRC010000052        10910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3099_length_10910_cov_2.533763, whole genome shotgun sequence.
ACCESSION   DAWDRC010000052 DAWDRC010000000
VERSION     DAWDRC010000052.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3099_length_10910_cov_2.533763"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    255..264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4889..4898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7876..7885
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9139..9148
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2736 a         2776 c         2683 g         2675 t
ORIGIN      
        1 atgaaaaatg tgaagtgcat ttctttttta aaattttttt accctattat ttcttatcaa
       61 attaacattg cccttaatta taagaaagga gaatataaat gaacaacata ttaggctcat
      121 caattaatat gccctgtaca cttttcgaaa ctattacttt attcgatgat ttttctgctg
      181 atgatatgca gtatggggat atggaagaac aagattttct ctcactcggc ttaagcgaca
      241 tctctgcaaa agttnnnnnn nnnnaagttg atccttaccg cctcattaaa taccattttc
      301 ctgggccgaa ttctacttat ggtgctttta gtgttccaac ttctggaaca aaaatatcac
      361 aaagtgaatg tattgatatt ttatttgcag aaatgaaaga tctggcaaaa atgttttcct
      421 tttttggaca atacaaaaca ctcatccagg atctgattga tcattttaga tatggaaatg
      481 gcaacagttt ccatagccag gaattgaact tatcttttca tgaacgaata aacaaatatg
      541 attataatag cccaatacgt gtaatcaaag aatgtatcga aaatgatatt tcttcaacac
      601 ctacgatagg ataccggcct cttctcctac aaaagattaa gaccgaatta ctttcatcaa
      661 gactaaataa attcaatgat tttaaagata attttaatgg cctgggaatc agcattcatg
      721 atatctcagc tcaaaaaata tctcttttaa attttcaaaa atatcctatg ggctggagcg
      781 ctaccattca tttcatagca caagaccact ttggtttgga tgtgactgat ataaaaaaca
      841 agatttacaa caagtataga ttttttcgca tatggttctt tttacaacgg cataaagact
      901 ttgctttcaa accttttttc accaatttta acactatcga aagaatcgaa aattatctat
      961 gaaaagaaaa ctccagaaaa ttatattata tttatttctt ctatgctgca tctataattt
     1021 atggacattg cgtcctgtac agattttgta tacatactct gatgctggca attcagtctt
     1081 tctggtcgtt gatcatctgc catggactga tagcgataaa ataaattggt atctgaagca
     1141 tcaggatgaa attaaaagtc aaaatcccct accagaaggt agttggcata cctggtatgt
     1201 tattgatatc ggtaatggct tcactgacta taaaaaatat atcgaagggc cgtatgaaga
     1261 tctctactgt ttcccgacga ttaagagcaa tgataattgc atcgtaaaaa actatttaat
     1321 ggttattaat gaatatcctt accgtaatac tcattttttt attgctgata acaatgagta
     1381 tcaactaacc caggaaaata agatagagcg agtgttcaac ccgcatgact ttgaaaaaga
     1441 caattttcag caataagccg gataaagaac aatctttacc cggcatttat attacaacca
     1501 gttcttccgc ttaaagtaca gatacggtgc caggcccgcg aggatcataa agataatcgc
     1561 gcctgggtag ccgaagctcc acttcagttc cggcataaac tcaaagttca tcccgtagct
     1621 ggaagcgacc agcgtcggtg gcaggaacac cacggatacc accgagaaga ttttgatgat
     1681 gcggttctgc tcgatattga taaagcccat cgccgcctgc atcaggaagt tcaccttctg
     1741 gaacagggat tcgttatgcg gcagcaggga ttcgatatct cgcaggattt cacgcgcctg
     1801 ctccagttgc ccacccggta agcgtgcttt acgtaccagg aagttgagcg cgcgctgggt
     1861 atccatcaga cacaggcgaa ctttccagcc gatatcttcc agttccgcca gcgtggagag
     1921 cgcctcgtcg tactcatcgc cctgatgccc ttccataatt acccggctca actgctccag
     1981 gtcgctgtag atattttcaa tttcatctgc caactgttcg attttggttt cgaacagatc
     2041 cagcagcagc tcgtaggcgt taccgtctac catcgactgg ctacgggcac gcatacggta
     2101 aaggcgaaaa gcaggcaatt cacgctcacg cagagtaaac agacgaccat cacggatggt
     2161 aaatgccaca gtggagttac cggcgtgatc ttccgcatct tcaaagaaga agaaggagtg
     2221 aatatgcagg ccgtcatcgt cttcaaagaa acgtgccgat gcttcgatgt cttccagttc
     2281 cgggcgggtt gccaggctct ggccaagttc agattgtacg cgcagtcgct cgtcgtcgtc
     2341 cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt cgacttccag
     2401 ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc
     2461 ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag accattaaac
     2521 agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag gctatcagca
     2581 aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag tatctactgg
     2641 gtgtgtccaa ggcgaatgtc ctcttagagt gatcgtgcgc gcatgttacg ccagcaaaat
     2701 tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcgc gctttttggt
     2761 actcgtcaag gtcgctaaca ggataaaaaa atcccacatc caggacctat aatggcgcat
     2821 cggctactca ttggtaaagg aatgatcacc ttgaatctca aacggatttt tctcgccctc
     2881 accttacttc ccttgtttgc tgttgccgct gatgattgcg cactctcaga tccgacgctg
     2941 accgtacagg cgtataccgt taatactcaa acagagcggg tgaaaatgta ctggcaaaaa
     3001 gccaatggcg aagcgtgggg aacgttacat gcgctgctgg cggatatgaa tagtcagggt
     3061 caggtgcaga tggcgatgaa cggcggcatc tatgatgaaa gctatgcgcc gctcggtttg
     3121 tacatcgaaa acggtcagca gaaggtggcg ttaaatctcg cttcaggtga agggaatttc
     3181 tttattcgtc ctggcggcgt gttttatgtc gcgggagata aagtcggcat cgttcgtctg
     3241 gatgccttta aaaccagtaa agagattcag tttgcggtgc agtcagggcc attgttgatg
     3301 gaaaacggtg taattaatcc gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac
     3361 ggtgttggga ttaataaaca tgggaacgcc gtgtttttgt tgagccagca ggcgacaaat
     3421 ttttatgatt ttgcctgtta tgccaaagca aaactgaacg ttgagcagct gctttatctt
     3481 gatggcacaa tttcacatat gtatatgaaa ggcgaggcaa tcccgtggca acgctatcct
     3541 tttgtcacca tgatttccgt cgagcagaaa ggttaaacat tgtacactcg aggaggccag
     3601 acaaactggg gctggctaca cagctggaaa atcatcagat aaatgaaggg aaacaaaaat
     3661 gaaaaaaacg ctcgcaacat tatttttatt aacctgtctg gggtcatcat ccgcttatgc
     3721 ggataacgct ttgattttac aaacggattt tagcctgaaa gatggtgccg tctcggcgat
     3781 gaaaggggtc gctttcggcg ttgaccataa tcttaaaata tttgatttaa cacacgaaat
     3841 tccaccggat aacatctggg aaggcgctta ccgcctgtat cagactgcca attactggcc
     3901 gcaaggttcg gtatttgtta gcgtggtcga tcctggtgtt ggtacagagc gtaaatcggt
     3961 agtgctgaaa accaaaaatg gtcagtactt cgtttcacca gataacggta cgctcacgtt
     4021 ggtggcggaa tcgctgggta ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgtct
     4081 gaaaggttcc gaaaagtcat ataccttcca tggtcgtgat gtatatgcct acaccggtgc
     4141 gcgtctggct tccggggcaa tcacgttcga gcaggtgggg ccagagcttc ctgcaaaagt
     4201 tgtcgaactt tcttatcaga aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc
     4261 gattctcgat attcagtacg gcaacgtctg gagcaatatc agcgacgagt tgctgaatca
     4321 ggcaggaatc aaactcaacg acacgttgtg tgtgaccatt tccgaaggtt cccagcaaaa
     4381 atacgcaggg aaaatgccgt atgtcgcaag cttcggcgat gtgccggaag gccagccgat
     4441 ggtgtatctg aacagcttat tgaatgtttc cgtggcgctg aatatggata atttcgcgca
     4501 gaaacatcag gttgcgtctg gtgctgactg gaatattgat gtgaagaagt gcgctaagta
     4561 agttactgat gccggatgcg gtgtggcgca ccgcatccgg caacggaatc gtaaattata
     4621 ccgtctccag tcgggcgtaa gccgccacca gccatttaat accgtggccc tggaacgcca
     4681 cctgcaaacg gctatgctca ccgctgcctt ccatattgac gatggtgcct tcaccaaact
     4741 tagcgtggcg tacgcgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac
     4801 ccatccgttg atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac
     4861 actcttccgg caactcaccg ataaagcgnn nnnnnnnnta ccgggtaggt cggtgtaagt
     4921 ttctggatcg cgcgagttac gccaacgtag gccagacggc gttcttcttc cagacgcccg
     4981 ccttcatcca gcgacatctg gcttgggaac atgccctctt ccataccaac gataaacacc
     5041 tgcgggaatt ccagaccttt cgccgagtgt agcgtcatca actgcaccgc atcctgccag
     5101 gtatccgcct gcccctcgcc cgcttccagc gccgcatggg agaggaatgc ctgcagcggc
     5161 attaaatctt cgtcttcttc gttgtagctg aactggcgcg ttgccgtcac cagttcctct
     5221 aagttttcga tacgcgtctg acctttttcg cccttctcct gctcgtacat ggtgcgcagg
     5281 ccggagtctt taattacccg gtcagtctgt acatgcagcg gcatatcggc agtttcctgc
     5341 gctaaggcgt cgatcagctc cataaaccgc tgtaaggcgc tggcagcacg tccggcgagg
     5401 gctttttcct gcaacagttc acgacatgcc tgccagagtg ttaactggcg atctcgcgat
     5461 gtctgacgta ccacgtccag cgtccggtca ccaatacccc gcgttggcgt attcaccaca
     5521 cgctcaaagg ccgcgtcgtc gttgcggttg gcaatcaggc gcagatacga gagcgcatct
     5581 ttgatttcct ggcgttcgaa gaagcgcatc ccgccgtaaa tacgatacgg catactcgcc
     5641 tgcaataacg cctcttccag cacacgcgac tgggcgttgc tgcggtagag aatggcgcac
     5701 tcggcaagcg ccccgccgtt gtcctgccag gttttgatgc ggttaaccac aaaacgcgct
     5761 tcgtcgagtt cgttaaaagc gcaatagagg gaaataggct caccgtccgc gccatcggtc
     5821 cacagttttt tacccagacg cccgttattg ttttcaatca gggcgttagc ggcgctcaga
     5881 atattgctgg tagagcggta gttctgctcc agacggatag tttcagcacc ggggaaatca
     5941 ttgaggaaac gctgaatatt ctcgacctgc gccccgcgcc agccgtagat tgactggtcg
     6001 tcatcgccaa cgatcatcac tttgccagta tcgcctgcca gcagtcggat ccacgcgtac
     6061 tgaatgttgt tagtgtcctg gaattcatcc accagaatat tggtgaaacg ttcgcggtaa
     6121 tgttgaagga tatgcggttt gttgagccat aattcgtggg cacgaagcag caattcagca
     6181 aaatcaacca gacccgcgcg gtcacaggct tcctgatacg cctgatacac cttctgccag
     6241 gtttgctcca ctgggttgcc gtagctctga atatgatgcg gacgcagccc ctcatctttc
     6301 tggctgttga tgtaccacat tgcctggcgc ggcggccact gcttctcgtc gagattcatc
     6361 gccttgatta ggcgtttgag caggcgtagc tggtcttcac tgtcgaggat ctggaaatcc
     6421 tgcggcaggt tggcgtccat atggtgcgca cgcagcaggc ggtgcgccag cccgtggaag
     6481 gtgccgaccc acataccgcc ctggctagtg cccatcagct gcccgatacg atgacgcatc
     6541 tccgccgccg ctttgttggt aaacgttacc gccataatcg agtatggcga gcagttttcc
     6601 acgctcatca accaggcgat acgatgcacc agtacgcgcg tcttaccact gcccgcgccc
     6661 gccagcacca gaaggttgct gcgtggcgcg gccaccgctt cgcgctgttt gtcattaagg
     6721 ctgtcgagca ggtaagaaac gtccattggc accgccgcgt aaaaataggt tgggcggaga
     6781 agcgcaacgc cctccgccaa aaagctgggt atatatacag atttgctgat tatatcagcg
     6841 aggtcagaga tgccaaccgg gaaatttcca gatgcggcag taaacggctg tcccaggttt
     6901 gcatcagatc gccattttcc ggtctgatcc aacaggcctg cattccgctg cgaattgccc
     6961 cacccacgtc agtggtgaga tcgtccccaa catgtaagat ctcgccgatc ggcacgttga
     7021 gtttttccgc cgccaggaag tacatatcgc tgaacggttt tgagcgcccg tgcgggccag
     7081 cgcgcagcac aaactcaaaa taatccccca aaccaaacag ttccggctgg gcgttaccgt
     7141 tggtgatcgc caccagcggc catttcttcg ccagctgttt taaggtgtcg tgggtttgct
     7201 gcgggacgtc gatccggctg cgccatttgg caaagttgat cattgccgcg tgtgcgcctg
     7261 cactggcttc ttcggcactc agcccggcgt cgagcatcgc ttgttcaatc gaacgaaaac
     7321 gccagcgcgt cacgtcgtga taaatctcgg gttccgcttc ccgtaccgcc tggcgcaggc
     7381 gttgcagatc ttcattctgg aagctgcgca gcgccggatg ataattttgc acaaaggtaa
     7441 gcgcctcctg ctcggtgcgc aaaatcaccg gacggttatc gtaaagggta tcatccaggt
     7501 caaaggtgag cgccgagatg cgccccaaag gccggtaaaa acgcattatt tcccccgttt
     7561 ggcgcgtgga tgcgccgcat cgtacaccga ggcaaggtgt tgaaaatcaa gatgagtata
     7621 gatttgcgtg gtggagaggt tggcatgacc cagcagctcc tgtacaccac gaagatcgcc
     7681 gctcgactcc agcatatggg tagcgaacga gtgacgtaat ttatgcgggt gaacgtgatt
     7741 attcagccct tgttttatgc cccattcggc aaagcgtttc tgcacattac gcgcggagat
     7801 gcgtttgccc agtttcgaca gaaaaagcgc gtcgtcttcg ctaccaaaca ggtcgcgcaa
     7861 atcaagccag tgctcnnnnn nnnnnccgat tcatatcatc gacgtcgatg tttttcggca
     7921 gatgacgcgg cgctttcggt gccgaaacac ctttcgccgg gttagctttg agttcgttct
     7981 ggctgaccag ccagtcaaaa aagctacgta gcgcagaaag ccgtaacgcc agacttgcag
     8041 ctcccagccc tttacggcga ctgcgtacag caaaattacg caccatcgtc acatcacatt
     8101 gctgccagct ttgcaggcca ttttcgctgg caaaattgat gatcgcctca agctgtcgct
     8161 ggtagttaag cagggttatc gggctaagct ggcgctccac gctcagataa cgtaggtagc
     8221 gttctacatc ggtgtgtaaa tcggtcatac gcgttcaatc caacgcgcca gaagctccgg
     8281 caacatcagt gcaatttcat gaagtaactg cgttccctgc ccttgttgat agtgactggc
     8341 atcgcgactg gtaaacagca caacacccaa atcagcatcg cttcccagca tcgacatcgc
     8401 caccgatccc accgctttcg cttccggtag caccaccagc agctccggtc cgttaagcgg
     8461 tcccagatag tgctgttcct gccccagacg ctgaatacgc agcggttcga aagactgacg
     8521 gcttaatgcc agatgagtgt agttcgacgg cgcacctaag cgccagcgat ccggaaacag
     8581 gcgcagactc gcacctgcca ggccgagatc gcgcgcccag cggtgaaagc gcatcagcat
     8641 atcatcgaga ctgctggcgg cggtgagact gcgctgcagg tagagcagac gataaaacag
     8701 gccttcgttg gcgattgcct gttccatcaa cagcgccatg ttctcttcca gaacatgaat
     8761 atgattacgt gcgcgggcca tgtgccactc gaccaacgaa acggtgccgc gtaccggatg
     8821 cggcacacgt atcgcttcta ctgcgcgcgc attgcggata aaaaactcag gatttttaat
     8881 cagataatcg acaaccgccc ggtcatcaag ctccgtgagt gtttcctgca gttcttcccc
     8941 tggttgcttc atagatgaat aaatccgtcg tagacatgta ccgccgggcc agtcatatat
     9001 aacgggtgac ccggaccttt ccaggcgata tcaagacgac cgccagggag ttccacgcgt
     9061 acttcttcgg ccagcaaacc ttgctgaatc cctaccgcca ccgccgcaca cgcgccgctg
     9121 ccgcaggcct gggtttctnn nnnnnnnntg ccccacgctc ataaacgcgt aaacgaatat
     9181 gctcgcgctt aaccacatgc ataaaaccga tattggcgcg ctccggaaaa cgctcgtggc
     9241 tttccagaac aggaccaagc gtttctaccg ccgcggtatc gacatcatcg acctgaatca
     9301 cgcaatgcgg atttcccatc gacaccacgc cgcataagat tgtctgctcg gcggcgcgca
     9361 taatataggt cttttccgct ttgttagcgc gaaacggcac ggcggaaggt tcgaagttgg
     9421 gttcgcccat atttacgcgg accagatcat catcggtgac ggtcagaacc atccgcccgt
     9481 tggcggtgct gacgcggata tcgcgcttat tggtcagccc tttcaggcgc acaaaacggg
     9541 caaagcagcg cgcgccgttg ccgcactgcg ccacttcact gccatcagca ttgaaaatgc
     9601 gatagtgaga atccagttca ggatcatacg gtggctcaac caccagcagc tggtcaaacc
     9661 ctacccccag gtgccgatcc gccaggcgac gaatcagatc cggtgaaaaa aagacattct
     9721 gcgttaccgc gtcgacgacc ataaaatcgt tgccaaggcc atgcattttc gagaactgca
     9781 tcatttactc caatcacgcg ggtacagaaa ctgactttta gtaattcacc tgggatggac
     9841 catcgccagt ggcgcggtcg tttttatccg gcaccgtgga ttgcgtttgc gtctctaccg
     9901 gtttggtcgg cggcggtgcg tttttatctg caggcgggaa atagagcgga cctttcagac
     9961 cgcagcccgt caggctgaag agagtaagta atacagtgag tgccttaaac acgtttttca
    10021 ttatggattg cctgtaaagt tcatcgcttt ctgctttcta tcatcgcagg agaaggcgta
    10081 aaagcaagcg tttagcgact ctttatgaag tctgaaaagt gaggaaatgc tgcgagtata
    10141 acgcttactc gtggcgattt atgttttgct ggtagtttcg tgatcggcac ctcgcgccag
    10201 gatggcttta tgcactatcg caatgtggtt tgtttattgt cttgttcgtt gttcttatca
    10261 tcggcatggg gatgtcggct tgatgagccg gaacataata tttaccagaa acagggaaaa
    10321 ggcgtggtgt atttgcgccc ttatgaaaag accaatcttt cgctatatag agatattatt
    10381 attccggtga cggatgaatc atgccggaac gcagcagcag acgggcaaac tttgaagccc
    10441 tgaccccatg ccgctatact gccgccatca cttaaaaaca ggatacaaca atgaacgaca
    10501 gtgaatttca tcgcctggct gatcaactgt ggctgaccat tgaagaacat ctggacgact
    10561 gggatggcga cagcgatatc gactgtgaaa tcaacggcgg tgtactaacc attacctttg
    10621 agaacggcag caaaatcatt atcaaccgcc aggaaccgct gcaccaggta tggcttgcaa
    10681 ccaaacaggg cggctaccat tttgatctga aaggcgatga gtggatttgc gatcgtagcg
    10741 gtgaaacctt ctgggatttg ctggaacagg cggcgacgca gcaggcggga gaaaccgtca
    10801 gttttcgtta agagctaatg ccggataagc ctcgctttcc ggcattagca tcaagaaaaa
    10861 tactgctgca acagcggcgc gtcatgctcc tgattggcag gtggcatgtt
//
LOCUS       DAWDRC010000053        10793 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3137_length_10793_cov_2.777519, whole genome shotgun sequence.
ACCESSION   DAWDRC010000053 DAWDRC010000000
VERSION     DAWDRC010000053.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10793)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10793)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3137_length_10793_cov_2.777519"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8848..8857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2706 a         2816 c         2596 g         2665 t
ORIGIN      
        1 aaaaaaagcg attcagaccc cgttggcaag ccgcgtggtt aactcatcca taaaatatcg
       61 cgcaatggcg ggcatcccct ttcgccccgc aaataaagca tacaacggtc tgggtatgcc
      121 gctccacggt gcaaacaggc gcaccagttc cccgttcgca agcccctgtt tacaggcaaa
      181 ctgaggcaat aacgccacgc cattcaacac aaccagggcg cgaactcgct gagcgaaatg
      241 cattagcgca aatccctgat cttttaagct gcgcgttaac cactgataca aatgttaccc
      301 gccaacggct tcggtatatg caacccgaca caaaattgtg tcatagtgca ggaaaaagca
      361 tttaccagga gcagacaaca gcaatgaaac gcccggacta cagaacatta caggcactgg
      421 atgcggtgat acgtgaacga ggatttgagc gcgcggcaca aaagctgtgc attacacaat
      481 cagccgtctc acagcgcatt aagcaactgg aaaatatgtt cgggcagccg ctgttagtgc
      541 gtaccgtacc gccgcgcccg acggaacaag ggcaaaaact gttggcgcta ctacgccagg
      601 tggagctgct ggaagaagag tggctgggcg atgaacaaac cggttcgact ccgctgctgc
      661 tttcactggc ggtcaacgcc gacagtctgg cgacgtggtt gcttccggca ctggctcctg
      721 tgttggctga ttcgcccatc cgcctcaact tgcaggtaga agatgaaacc cgcactcagg
      781 aacgtctgcg tcgcggcgaa gtggtcgtgg cagtgagtat tcaacatcag gcgctgccga
      841 gttgtcttgt cgataaactc ggtgcgctcg actatctgtt cgtcagctca aaaccgtttg
      901 ccgaaaaata tttccctaac ggcgtaacgc gttcggcatt actgaaagcg ccagtggtcg
      961 cgtttgacca tcttgacgat atgcaccagg cctttttgca gcaaaatttc gatctgcctc
     1021 ctggcagcgt accctgccat atcgttaatt cttcggaagc gttcgtgcaa cttgctcgtc
     1081 agggcaccac ctgctgtatg atcccgcacc tgcaaatcga gaaagagctg gccagcggcg
     1141 aactgattga cttaacgcca gggctattcc aacgccggat gctctactgg caccgctttg
     1201 ctcctgaaag ccgcatgatg cgtaaagtca ctgatgcgtt gctcgattat ggtcacaaag
     1261 ttcttcgtca ggattaatcc atcaaataat gcctgatagc acatatcagg cgttatcctc
     1321 acttcttttt gtattccttg aatcacatta caaaatagac aaatctcagg cggcaaaaaa
     1381 cgacgtctga atgcattttt ttgctggaga caaacccacg taaaaagctc accgtaagcg
     1441 caaataccct cattttgatt gcgttttacg gagcaaataa tgtctaacgt gcaggagtgg
     1501 caacagcttg ccaacaagga attgagccgt cgggagaaaa ctgtcgactc gctggttcag
     1561 caaaccgcgg aagggatcgc catcaagccg ctgtataccg aagccgatct cgataatctg
     1621 gaagtgacgg gtacccttcc cggtttacca ccctacgttc gtggcccgcg tgccactatg
     1681 tataccgccc aaccgtggac catccgtcag tatgctggtt tctcgacagc aaaagagtcc
     1741 aacgcttttt atcgccgtaa cctggccgcc caccgaggct acgactccga taacccacgc
     1801 gtggcgggcg acgtcggcaa agcgggcgtc gctatcgaca ccggggaaga tatgaaagtc
     1861 ctgttcgacc agatcccgct ggataaaatg tcggtttcta tgaccatgaa tggcgcagtg
     1921 ctaccagtac tggcgtttta tatcgtcgcc gcagaagagc aaggtgttac atccgataaa
     1981 ctgaccggca ccattcagaa cgatatcctc aaagagtacc tctgccgcaa tacctatatt
     2041 tacccgccaa aaccgtcaat gcgtattatc gcagacatca tcgcctggtg ttccggcaac
     2101 atgccgcgat ttaataccat cagtatcagc ggttaccaca tgggtgaagc gggtgccaac
     2161 tgcgtgcagc aggtagcatt tacgctcgcc gataggattg agtacatcaa agcagcaatc
     2221 tccgccgggc tgaaaattga tggcttcgtc cctcgcctgt cgtttttctt cggcattggc
     2281 atggatctgt ttatgaacgc cgccatgttg cgtgcggcac gttatttatg gagcgaagcg
     2341 gtcagtggat ttggcgcaca ggatccgtat aacaacgtta tccgcaccac cattgatcat
     2401 tgtgcacatc ccatgtatcg ggattatctg catcgatatc tggaaaatgc gtccggcgga
     2461 catattcacc acgatcttag ccacgtcttc gatttacacc gtaatttaat tgcaaccggc
     2521 tcgatgctgg gttaatgact cgtcccgtca cgatcttcag caatatgctg aagcatcgtg
     2581 gcggtataac gatgatttct gattgaataa agatattcct gcatatacat cggcctgtgc
     2641 ggcgcatcaa aatatttcag cgttgccgga ttcaccagtc gccaggcaaa atgcgggatc
     2701 accgtcagaa aatgtccccg ctccacggcg ctgattttgg ccataaaact atagggacga
     2761 taaataatgg ttggattgat cccacaagga cgcatattcg catcaatcat tgcttcgaag
     2821 ttagcccggt tttgaaagcg catttgtagc cagggcagct cctgaagcag ctcttgctcc
     2881 tgtcgccctt cataacggcg agaaacgtga aagcccaatc gcagcggtgg tagttcactg
     2941 atagtcaggt tttccagttc ctgaactcgt gcagatacat gctgtgggga gattataaaa
     3001 tctaactgac ggtcaaaaag attatcgata acgccatttt cgctgaactc aactggctgg
     3061 gcggtcacgc cttcgtactt atctcagaga ctaatcaact gatcaaaaat aatggtcgga
     3121 taagtattat ctatgcctat cacaatgtct cgtgaacaac gtcctgagtt atggatttca
     3181 ttatcaatcg cagaaagtcg ttgataaacc gggaacagtt tttgatacag ttcctgtccg
     3241 gctttattga ggctgatatt attatctttg cgggtaaata gcgtatagcc aatttgttct
     3301 tccagcgcgg caatgctttt gccaaatcgc gaagccgtca tatggatttt ttctgccgcc
     3361 cgggcaatat tattggtttg cgctaataaa atgaaattgc gcatcttttt cgaaataaaa
     3421 atgtccatct cccctctccc gcatccttgt ccgcttaacc gtttcagtca gcctatcctt
     3481 gatgaaaccg cgagcaaaga taggtgatta cgtcatggtt ttacagaaaa aggaggcaat
     3541 atcgggtaaa ggcattagcc cgacgaatac gtcgggctac aagtattaat gtgcagcagg
     3601 tgttttagcg ggtggttgat ccacaggttc taactggaag actacatcga cctgatcatc
     3661 aaactgaata gcggcctgct cgtaagtttc ctgtgcggac accggtgcag catcagcttt
     3721 catcatccgc accattgggc tgggctgata gttggaaaca tgataacgca cgctatatac
     3781 cggcccgagt ttgcgattaa agccattcgc cagttcctgc gcctgatgaa tcgcgttatc
     3841 aatcgctgcc ttacgcgctt tgtctttata ggcatccggc tgcgccacgc ccagcgacac
     3901 agaacgaatt tcgttaagac ccgcctttag cgcgccgtcc agcaaggaat tcagtttgtc
     3961 taactgacgg agcgtgactt ccaccgttct cactgcgcgg tagcctttca ggatactttt
     4021 accatcctga taatcataat ctggctgagt gcgtaagttc gctgagctga tatctttttt
     4081 cgcgatctga ttgagctcaa ggaaggaaat gtattgtgcg acgcgctcat ctgcctgttt
     4141 cttggcagta gcggcatcct tcgcggccac gttaacttca atcgcaagag tggcaatgtc
     4201 tggcaccgca tccacgcttg ccgtaccgga ggtgacaata tgcggtccat ccggcaattc
     4261 gttagcctgc gctgccatcc cgctaatacc cattaatgcc gccagggcga taactttgaa
     4321 cttcacgtta gtctctccgt gttaattgac ttgcccaaaa ttcaggacct ctggaggcaa
     4381 gcttagcgcc tctgttttat ttttccatca gatagcgcct aactgaacaa ggcttgtgca
     4441 tgagcaatac cgtctctcgc cagctgcaag gcaataaacc acataacaca tcccactacc
     4501 agattgataa tgcgctgtga ttttgccgtg cgcaggcgcg gtgccagcca ggctgcgaga
     4561 atagccagac caaagaacca caggaaagag gcgctaattg ttccgagcgc aaaccaacgt
     4621 tttggttcca catcaagttg cccgccaagg ctgcccagca caacaaaagt atccaggtaa
     4681 acatgcggat tcagccaggt cactgccaac atggtggcga taattttcca tctgccttgt
     4741 ttcagaactt cggcactggc taactcaatg ttactgctca ttgctgtttt aaaagcgcca
     4801 aaaccatacc acagcaagaa ggctacgccg ccccaggtga ccagcgccag caaccacggc
     4861 gactgcatca ataacgcgct gccaccaaaa atccccgcac aaatcaggac caaatcgctg
     4921 atagcacaaa gtaaggcaat cataatgtgg tactgacgac gaatgccctg attcatcaca
     4981 aaagcatttt gtggaccgag cggtaggatc atagccgccc caagtgcaag accttgaaag
     5041 taataagaaa acacgttgga tattccgaat taagtatctg atgcagagac tatatcgcgg
     5101 gattgttatt agcggaaatt gataatatta attggcaatc agaaaaacta ataagagaga
     5161 ggccagagag tattatctgg cctcagcgtt gattacgcag ctttgtcttc tttcacccgc
     5221 ttaaagttca catccatttg cgtgtacggg aagctgatac cggcggcatc aaattcacgt
     5281 ttaatacgct ccagcacatc ccagtacacg ttttgcagat cgccgctgtt gctccagacg
     5341 cggaccacga aattaatcga cgatgcgcca agttcgttca gacgcacagt catttcgcga
     5401 tctttcaaaa tgcgatcttc agactggata atatcggtca ggatctgctt aacctgatcg
     5461 atatcggaat catacgccac gccaataata aattcgttac ggcgagctgg ctcgcgggag
     5521 aagttaataa tattcccggc aataatttta ccgttcggaa taacgataat tttaccgtct
     5581 gcagtacgca tggtggtgga gaaaatctgc acactcagca cagtaccggc tacgccgccg
     5641 aggtcaacat attctccggc acggaacggg cggaacatga caagtaacac gccagcggcc
     5701 aggttagaca gtgacccctg caaagccaga ccaacagcta agcctgcggc accgagtaca
     5761 gcaatgactg acgctgtttg tacgcccacg cgccccagtg cagcgattag cgtaaaggcg
     5821 ataattccgt aacggactaa tgcagaaaga aaatcagcaa cagtggcatc gattttacgg
     5881 gagatcatca ggcgattcac cgcgttggaa atcatccgcg cgataatcaa accaacgatg
     5941 atgatcgcga gtgccgccac gatgtttact gcataactta gcagcagcgc ctggttagcc
     6001 accagccagc ttcccgcgcc gtttatgcta tcgacaacat tcaaatcttc cattcaatat
     6061 tccttttcaa ctgactcaaa atggagaaat acactccgcc gccttatgac gggcagtctg
     6121 acaagggtaa acaataaagc ataattttgc caaatagatc acagatttct catctgtatc
     6181 accttgcttt tggcgaaaaa aagacccgca gagcgggcct tgagataagc agaaaggaat
     6241 atcttacaga acgtcgatcg cgttcagttc ctggaatgct ttttccagac gagcgatcat
     6301 cgaagtctga ccggcacgca gccatacgcg cggatcgtag tatttcttgt tcggctgatc
     6361 ttcgcctttc gggttgccca gctggccttg caggtaagct tcgttttctt tgtagtagtt
     6421 cagaacacct tcccaggttg cccattgggt atcggtatcg atgttcattt tcactacgcc
     6481 gtagcttacg gagtctttga tttcctgagc agtagaaccg gaaccaccgt ggaatacgaa
     6541 gttcaggctg ttgtgcggca ggttgtgttt cttagaaaca tattcctgag agtcgcgcag
     6601 gatggtcgga gtcagaacca cgttacccgg cttgtaaaca ccgtgtacgt taccgaagga
     6661 cgcagcgatg gtgaaacgcg ggctgatttt gctcagctcg gtgtatgcgt aatcaacgtc
     6721 ttccggctgg gtgtacagtg cagaagcgtc catgtggctg ttgtccacgc cgtcttcttc
     6781 accaccggtg caacccagtt cgatttccag agtcatgccg attttggaca tgcgctccag
     6841 gtatttagag cagatttcaa tgttctcttg cagagattct tcagacaggt caatcatgtg
     6901 agaagagaac agcggcttac cggtagcagc gaagtgtttt tcacccgcgt ccagcagacc
     6961 gtcgatccac ggcagcagtt ttttcgcgca gtggtcagtg tgcaggatca ccggaacacc
     7021 gtagtgttca gccatctggt gaacgtgatg cgcaccagag atcgcaccca ggatagcagc
     7081 gccctgcgga acgtcagatt tcacgccttt accagcgata aaagaagcac caccgttgga
     7141 gaactgaacg ataaccggcg ctttaacttt agctgcggtt tccagtacgg cgttgatgga
     7201 gtcagtaccg acgcagttta ctgctggcag tgcgaagttg ttttcttttg ctacctggaa
     7261 aactttctgt acgtcatcac cagtgattac gccaggtttt acgaaatcaa aaatcttaga
     7321 catgtctctt gtcctgtatc gtcgggcctt atactcgtca tacttcaagt tgcatgtgct
     7381 gcgtctacgt tcgctcaccc caatcactta cttatgtaag ctcctgggga ttcactctct
     7441 tgtcgccttc ctgcaactcg aattatttag agtatgaaaa agtgcgtgct ctaaaagcgc
     7501 gctgaaacaa gggcaggttt ccctgccctg tgatttttta cttcttagcg cgctcttcga
     7561 gcatcgctac tgcaggcagt actttacctt ccacgaattc gaggaatgcg ccgccgccag
     7621 tggagatgta ggagattttg tcagcaatgc cgaacaggtc gattgctgcc agagtgtcgc
     7681 cgccgccagc gatggagaac gcttcgctgt ctgcgatagc gttagccacg atttcagtac
     7741 ctttgcggaa gttcgggaat tcgaacacgc caaccggacc gttccacaga atggttttcg
     7801 cattcttcag gatttcagcc agttcctgag cggaagcatc accgatatcc aggatctgct
     7861 cgtcagcttt cacatcgtta acagatttca gggtagccgg tgcagtttca gagaactcgg
     7921 ttgctacgcg aacatcagac ggaaccggga tgttgcaggt ggtcagcaga cgtttagctt
     7981 cgtcaaccag gtcagcttcg tacagggatt tacccacatc gtggccttgt gctgcgataa
     8041 aggtgttagc aataccacca ccaacgatca gctggtcagc gattttagac agggagtcca
     8101 gaacggtcag tttggtagat actttagaac caccaacgat agccaccatc gggcgagcag
     8161 gttctttcag tgctttaccc agcgcgtcca gttcagctgc cagcagcggg cctgcgcacg
     8221 caacgtcagc gaatttaccg ataccgtgag tagaagcctg cgcgcggtga gcagtaccga
     8281 atgcgtccat tacgaacacg tcacacagtg cagcgtattt tttggacaga gtttcgtcat
     8341 ctttcttctc gcctttgttg aagcgaacgt tttccagaac aaccagttca ccttcagcaa
     8401 cgtcaacgcc gtcgaggtaa tctttaacca gacgaaccgg gttagacagt ttgtctttca
     8461 ggtagttaac aaccggcagc agagagaatt cttcgttgta ctcgccttcg gtaggacgac
     8521 ccaggtggga agttaccatc acttttgcgc cttgtttcag ggccagttcg atggtcggca
     8581 gagaagcacg gatacgcgcg tcgctggtta ctttcccgtc ttttactggt acgttcagat
     8641 ccgcacggat aaatacacgt ttcccagcaa gatccagatc ggtcatctta attacagaca
     8701 tggtgaatcc tctcgttgat tctaaaagtt ttgcagacgc tgcttgcgtc ttacctgaaa
     8761 gcaacagtag ccatagctaa cgtcgtgtcg agcattcggt tagcaaagcc ccattcgtta
     8821 tcgcaccaga ccaacgtttt gatcaggnnn nnnnnnntga tcaggtgtgc gccactgacc
     8881 cgggtttgag tgccatcgac aatggcactg tgcgaatcgt ggttaaaatc tacagagacc
     8941 aacggcaatt ccgtatagtc aactatacca tgaaatgcac cttgtgctgc tttttgcagc
     9001 aacaggttga cttcattggc ttttacaggt ttcttcaccg tcacgcttaa atcgattgcc
     9061 gtcacattta tggttggcac acgtaccgca atcgcctcaa agcgatcgtt aaattgcgga
     9121 aaaaatcgtg tgataccggc ggccagttta gtatcgaccg gaatgatcga ctgactggct
     9181 gcccgagtgc gacgcaggtc aggatggtat gcatcaataa cctgttgatc gtgcatggcg
     9241 gagtgaattg tggtcacagt gccggactca ataccgtacg catcatctaa caatttgatg
     9301 acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa cgatgcggtg ttccgcacga
     9361 agttgatcct gattgacgcc ataaacaacg gtcgtgtcga gatcgttact gccaggatgt
     9421 gaaaagagca cttttttagc cccggcggca atgtgtgctt caccatgctc gcgggagcca
     9481 tatacgccgg tgcagtcgag gactacatca acgccaagtt cacgccaggg gagcgattgc
     9541 agtgaacgtt catgcaatac acggatggcg tcatcaccaa caaaaagttg atcgcgttcc
     9601 tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat atttcaacaa atgcgccatg
     9661 cccgcagcat ccgccagttc gttgattgcc accacggtaa tttccgcacg gcgtccggat
     9721 tcatacaaag cacgaaccac attacgcccg atgcgaccga agccatttat cgctacgcgt
     9781 acggtcatag atctcctgca aggttttccc tgagcaaatt tgccagacag agtaatccag
     9841 caaatcgttc ggggaaacct tacctgtcgc aaactgcgac tgattggtta attgtcgaac
     9901 atttaatcga ctgaaacgct tcagctagga taagcgaaac gtggaataaa aggaatgatt
     9961 gtccagtcaa agaagacaat tatctgactt acgtcacgtt ttcagccccg cccctgcgga
    10021 atttattcca ctttgcagaa tcacgaataa gcgtagcaaa atcttttcgt tgtgccgtca
    10081 caaaccctga cttatatcag aatatgactg atgcagcgtc gatattatcc gcaatacgtc
    10141 cgagtttcgg agtactccaa tgatgacatt gccctaccca tattcttcct ccgcacgttt
    10201 ttacgcgctg cgcctgctcc ccgggcagga agttctctcc caattacgcg ccttcgcgca
    10261 gcaacaacaa ctccacgccg cgtggattgc aggttgtacc ggcagtctga ctgacgtcgc
    10321 cttacgctat gccgggcaag aaaacactgc cctgctaagc ggtaagttcg aagtcatcgc
    10381 attgaacggc acgctggaac agagtggtga acatcttcat ctctgcgttt ccgatccaca
    10441 cggcacgatg cttggcggtc atatgatgcc cggctgtacc gtgcggacca cgcttgaact
    10501 ggtgatcggc tgccttgagg aactggcgtt tagccgccag tcttgcgcgc tttctggtta
    10561 cgacgagttg catatttccc ccgttaaata actactttca gtgaggtttt cttatggcgc
    10621 gtcgccactt ttccagtcag gcattggtgt tgatcgttat ctctatcgcc atcaatatga
    10681 tcggcgggca actggccagc atggtcaaat tacctatttt tctcgactcc atcggcacac
    10741 ttattagcgc ggtactgctt ggtccggtga tcgggatgct aaccggttta ctg
//
LOCUS       DAWDRC010000054        10474 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3243_length_10474_cov_2.207601, whole genome shotgun sequence.
ACCESSION   DAWDRC010000054 DAWDRC010000000
VERSION     DAWDRC010000054.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3243_length_10474_cov_2.207601"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    258..267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1069..1078
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2590..2599
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7668..7677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8581..8590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2444 a         2572 c         2686 g         2722 t
ORIGIN      
        1 tgggactaat ctgtccgcta actcttcctg gcttagcccg cgattttctg cgaccacctg
       61 aagaaattcc gccgcgttat cacgtaagga gcgggatttc gcccgttggg atatatggtg
      121 cagctgtatc agcgccatat cattattcat cagggtaagg atgttcaggc cactgacagc
      181 acgggcagat ttgccttctt gtggccaggc gaggatctgt gtcgtcagat cccgagccgt
      241 gctttcgtca ccaaagannn nnnnnnntgc cagcgtttgc ggctggcaaa acgttttcag
      301 ctgttccagt ccgccataaa aacgtccgcc ctgggtaaag cgcagcattt cgccgataat
      361 ttccagagcg tcatcggtaa caggctgatt attcttgata aggcgcggcc tggaccacat
      421 tgccggatgc cagaaatcag gagcttttgg aatacgtgcc gggtaaattt ctattggacc
      481 cagtttaagt aattgctcca gggcaggcca tacatctgta cgctgccagc ggtttgccac
      541 ggtttgcagt aattcgccat gcccttgttc atacagtaaa cgcagggcaa gtaatgcagc
      601 ttcgctatta tcgctgggtt ttgtaaagac aagagggata agtgcagtag ccgtatgttc
      661 cggccattgt aaaatccact ggcgagccag atcacgctgc gccgcgaaac gtcgccagac
      721 gcgggcaacg ggcagggcca gttctgttgc gccgaaattt aaaattaacg gaaatgtttc
      781 ttttggatga tgtgaaaaag ccaataacag gccaggtaat gtatccaacc cgaggatact
      841 gagaaaatag ttctcacccg cgaacagatg ctttttctca ttgatacgca gccagaaagt
      901 caaagccatc tcacgcggca actgtgccaa taaatacagg ttccagacac tcttttccgg
      961 acgataacgt gagtagctat cattttccca ggtgttaatt aacgcttcaa cgtcgccgga
     1021 acgaagcgcc tgttcggctg attttgcttc cgtggagtgc cagccattnn nnnnnnnngg
     1081 gagtaatgcc actgacccac taatgatttg cgcgccgcat ccagtttgcc gggcagtata
     1141 taattccgga acagccatac atcatcccca aaacctagct cctgtagtac aactcgacca
     1201 ctttttcgtt ccatgcgttc cgtaaaagat cccgtgctga aataacgggc agactctgtg
     1261 gcggcaagtc tctctcgttc gcctggttgc cagcgggctt tgggcgctaa ttcaagaggc
     1321 actaaatcaa ggcgcagcaa tgccgttttt ttcttacctc gccacggtgg agaaacaagt
     1381 atttctggca ggtcgttgct atgagtactc tcgatagcaa gttttggcga tgccgataaa
     1441 taaccattca ccactgcctg ggcttgtgta cttaagcacg gcatcacctc ttctgccagt
     1501 tgtggctgcg atgccatcag cgtttgtaac agcgtatgcc agtattcatc acgtgctttt
     1561 tgctgtgcag gcagcttttt gtcttcgatg atgggataac cagggcgcgc agggggggct
     1621 tttaacgcca gcagttctgc cagtgcggcg agcgtcgcat gggggaagtt tttactgtat
     1681 ttagccacac gttgcaggct gggtttgttt ttatcagcca ccagtagtaa ggtgcggatg
     1741 acttgcggat ggttaatttg taccagcaga ctgccgcaat cttctttatg tgcatacatc
     1801 gcgaggcgcg gaacggctgc cagtccttgt tcgcgcaaaa gggcagcgca ggccgcgtaa
     1861 ccgaagtggt tttcatgcga catgtagctg gcttctcgat cgctaaatat atcctggctc
     1921 cagtaacgtg cgagttcttt caccgcctta gggtcatcag caacaacttt taaccactct
     1981 tttgagcgaa aatgagtttg cgaacattca aggcttacca gctcgttggc gatttcgggt
     2041 ttttccggga ggttcagcgc aataaaaggg cggcgaactt tggttattcc tggcagtgcg
     2101 gcaatgagtt tgtcggcgca acgctgccag cagctttcct ctgccagaga gagatgctta
     2161 cgcaatcgga gatcgaattc gttataggtt tctgagcgat aaccatagcc atagtcctcg
     2221 ttctgatatt gcaatgttac taccagagga tcactctcat aacaccgtac aataaactgg
     2281 cgtgcaatca ccacctcagt tgcatattcc agcccctctt gttggacgat ttcgtcgagc
     2341 aactgttggc tatcattttg cgcgatacag gctagcgccg ccattgtccg ggccgtgatt
     2401 gagggtttgt gttcgccgat caagttgatg gcctgcgtga tggcggactg ccactccagt
     2461 gaaaagtttt cataacgcca gacaatgttt tttttcttct ctgctttttg tagcaattca
     2521 tcacccagtg tctgaatgat gctttcagtg ataacaggca ccggttcgcc tggccaattg
     2581 cgatggctgn nnnnnnnnng ctgagtggtg ttttgccttt tttatatttc agttccagtt
     2641 gggcgttatc cgccagccac ggtaattcct tgtccatgct ctttccttat tcaaacaggg
     2701 cttcaatatc gttaattagc tcgctggcgg tgatggcatc gagtacggag aactgcgctt
     2761 cctgtgttga attactcccc cagccatagc tttccgcttt gccttcccat agccacagct
     2821 tgcttaagag ttgctcggca ctgagttcgg ctggcgacat gccgaccgca aaaccttcat
     2881 cgatttccat gattaacgac cagcggccca gaggtttgat catccagccg atccagccac
     2941 cgtcctgcgg ttcaccacgc atccagcctt tatttgccag ccccattacc cgaccactcg
     3001 ggcattttct gcctgcccag cgtgtcagtt cgctggcatt gcgctcggct tctgtcagcg
     3061 cgtaactgtt acgatcaagc tggcggaacg gtggtaacag ttcgtagtcg gcaaaaagct
     3121 gcccgaaggc ggcagcatcc gtcggtgata tttccagaac gtgaggaata ccaatagaga
     3181 tatcgccttc cggcagggtg aaaagatcat cgtcagcggt gctgtagctg ttatcttccg
     3241 ctacgcgaaa gcaagccagt agctggtttt cggcgctata aacgccccaa atcagacggc
     3301 gggttaagtg gcggaccagc ggatgctcaa ccaggaagag ctgaaagttt tccggtgacc
     3361 agcggcgacg caggcacatg gcggattcca gccttgctac ctgctgggcg gcgacggtac
     3421 gcgcatcttt tttcagcaat ttgtagcggt taaccgcatc gttcgcctgt gattcatcat
     3481 cgcttttgtt tggtttgggc aggtctttca gacgactgcc ggaagcgtca cgcacaaacg
     3541 gttttaaggt ttcatcaaag ctgacggtga actggcgtgg gccgaaatcc agcagcaacg
     3601 aaccgttatc atccagaccg agatccggtg ctaaccgatc ttcaagctcc gccaccgtga
     3661 gttcgcggct ctcggcaatg ttggctattt tttcttttgc ccgctcctgt aatgctttga
     3721 atttcagttt ctgggcgatg ccgttaagct gcatcagggc gatatcacta ccgatagcag
     3781 cgagaatatc caacccgacg gtggcgcgtt tatgctggga ttcaccaggc caggcgcgaa
     3841 tcaaaggcgt cagtttgcgg gcggtgtcat cgttaccgag aacgcctaac gcagtgaacg
     3901 cccaactctc tttcgacggc gcgccagcgg tctgccaggc ggtaaacaga tcccaggcaa
     3961 attcagccag tgagtcggtg gtacaggcat ctttcacctg caataatccc ggatacagtg
     4021 cctcttcctg agggaagcgg agcatttcac cgaggtggag gagtgcgcta tcaggcagtg
     4081 attgggcgtt tgcttttaat aacgggcgcg tccagagcga tggctgataa aaggtcggaa
     4141 gagtggggat ttttgtcggg tgattatcta agggatcgag cgcaagtaga gcgttcaccg
     4201 catcggttac ttccggctgg ttataacgcc gggcgatttc ttgcagcaat ggctgatgac
     4261 cgttttcggt aagcatacgc aaggcagcac gggcgttgtc ctgtgcttca ctggctttgc
     4321 cgagcgctgc aggcagcagg ccggtaatgg cgtgttccgg gtatttcagc agccagctac
     4381 tggcgtcttg tcgtaatgtt ttgagtttgt tgaaggcgcg ggcgacggca ggagccagtt
     4441 ccgttgcggc aaaataatta gcaaccgcaa aaccttcttg agggtagcgt gaaaacgagt
     4501 gtagaaaacc actcatacct gccagtcgca aataaagcat gactggtttt atcgcggtat
     4561 gcggctcttt gctgagaaat tcccatagct taatcgcttt ttgcataggt aaaaagggaa
     4621 gtgagttgag atgccactcc ccctgatacg gtgcatggtg ggctttaaac atttggatga
     4681 gtgtagcgaa gtcctcacgt tcccatgcgt caattgccga gtcagaagcg ccgatatatt
     4741 tatatgtgtc aaaatcccag cggcgatacc ccagcgagta aaggaaattt ttagcacctt
     4801 cacctggctt atgcggttcc cagtcccaat gagtttgaaa ctctttagct tctgtgtcgg
     4861 ttattgtgca tatggattcg aggttgagcg gtgctaaatc cagcaccaga atcggcgatt
     4921 ttttcttttt cgaaagccat ggtggggaga ctactatggc cggcagtaga tcggcgctgg
     4981 catggtttga gggctgtgtc agttgttgct ggcatgattt cagcactgca actgcgggtg
     5041 tcgaaagcca gggaatgacc tgttctgcta gtgtcggttg cgagataagc atcgtcatta
     5101 gcataatgcg ccaactattc tcttcttttt gcactagaag ttccgccagt gctgccagtg
     5161 ctgcgtgtgg gaacgcagcg caggctttcg tcattcgatc gtgacagcgt ttagtatgcc
     5221 ctgctacacg tataagcagt gttaatgcga acggatgatt gatatgacgc aacacatcgg
     5281 cgcagtagtc acttgcgaca tagggagcaa atcggggcag ggccgcaaca ccttgctcct
     5341 gtaatacggt ggctgaccat atcgcaccac aatagtaatc atcaaaaatg gcatatggct
     5401 cgtgatattt ttctaatgag gcaagaatgt gctcatcagt cgccactatt tttagccact
     5461 ccagcgaggg taatttcttt tgccccagta gacgctgggc aatttcatgt gcaatttctg
     5521 gtttttcggg caataacaaa gcaattaatg gttgccgcca ttctggaata tgtggaactg
     5581 cggcaataag tttttgtgca catttttgcc agagagactc ttccgccagt gataaatgtt
     5641 tacgtaggcg taattcaaag ctggaatatt gctgcaaata atcaggtgaa atgccagacg
     5701 acaggaaaac aacattattg ttgacataat cccattcaat agatatttgt tgaagatgta
     5761 ttattacatc agtagcatat tccagaccgc cttcctgaat aatctgatcc ataacatcct
     5821 cacgctcttt atatcttgtg catttcattt caagtgccgt gagtacagcc attacgggtg
     5881 atggtagctg tgttgttttc aggtcgtcga tctgactgat cgcctgggcg acggtgtttt
     5941 gccattctaa agaacaggcg ctgaagtcaa atgtggctgt tgtattatcc cgttgcgaca
     6001 cactacaggc taagcgacgt aattgggtca atttgtctgc ttgctgaaca ggaactccgg
     6061 gaaagcggcg atgacttaac gtggtgtgta caacttcggg cggaaaaacg acagcaatat
     6121 ctttcgccag ccacggtaaa acagcatctg tatttttgtc agccgcacgg gtttctgcta
     6181 cgaaagaggg ttgttcctga ataatggtgg ataagtccgc taccttgaga gagtccgtta
     6241 ccgtttggga aggttgcaaa gaattatcct tcgcttgctc cacatatccc ttcttcacct
     6301 tctccgcaat cagcttcagc tccgcttttt ccgctgccac agcatcagca aaacttttta
     6361 cctggctttg cccttgggta ccaacttttc cccaactgat atgcaactcg tttccctgtt
     6421 gctccaccgc ccagaattta tgtgattttt cgtcctgata gataaagtgt ctcattctgc
     6481 atccctgtaa ttttatccag ttgaatgcag atgctaccag tatttatgcg ggttagagag
     6541 agacaaaatc gcaagagaac aactttagga atttataaag aaaaggcgct gtcgatgcag
     6601 cgccttgaag ggggattatt tcacctgatg acccggttta gcaccggcat ccgggcttag
     6661 caggaagata tctttcccac caggaccagc agccattacc atgccttcgg agataccgaa
     6721 gcgcatttta cgtggtgcca ggttagccac cataatggtg tgacgaccaa ttagtgcttg
     6781 cggatccggg taagcagaac gaatgccgga gaagacattg cgtttttcac cgccgagatc
     6841 cagcgtcagg cgcagcagtt tgtcagaacc ttcaacaaac tctgcgtttt caatcagcgc
     6901 cacgcgcagg tcaactttgg cgaagtcgtc aaaggtgatg gtttcctgaa tcggatcatc
     6961 tgccagtggg ccagttaccg gtgcggctgt ggcttttact tcctctttgg atgcttccac
     7021 cagtgcttca acctgtttca tatcgatgcg gttatacagc gccttgaatg gattcacttt
     7081 atggcccaac agcggttgct ggataccatc ccaggtcagt tctgtattga ggaatgcttc
     7141 tgcgcgctcg gtcagtttcg gcagtaccgg tttcaggtaa gtcatcagca cgcggaacag
     7201 gttgatgccc attgagcaaa tcgcctgcag atcggcatcg cgaccttcct gcttcgccac
     7261 cacccacgga gcctgttcat cgacatagcg gttagccagg tcagccagcg ccatgatttc
     7321 acggatagct ttaccaaatt cgcggctttc ccacgcttca ccaatcactt cagcggcatc
     7381 agtgaaggtt ttgtacaact gcgggtcagc cagctcgctt gccagcacac cgtcaaaacg
     7441 cttattgata aagccagcgt tacgggacgc caggttaacc actttgttca cgatatcggc
     7501 attcacgcgc tgaacgaaat cttccaggtt gagatcgata tcatcaatgc gcgaagacag
     7561 tttggcggtg tagtagtagc gcaggctgtc ggcgtcaaaa tgattcagcc aggtgctggc
     7621 tttaataaag gtgccgcgag acttggacat tttcgcgccg ttcaccgnnn nnnnnnnaaa
     7681 caggttggtc ggcttgcgga agttgctgcc ttccagcatg gcaggccaga acaggctgtg
     7741 gaagtaaacg atatctttac cgatgaagtg atacagctcg gcagtggaat ctttcttcca
     7801 gtatttatcg aagcttacgc tgtcgccgcg cttgtcacac agattcttga aagagcccat
     7861 atagccaatc ggtgcgtcca gccagacata gaaatatttg cccggcgcgt tcggaatttc
     7921 aaaaccgaaa taaggcgcgt cgcgggagat attcctctgt tgcaggccag attcaaacca
     7981 ctcctgcatt ttatttgcca cctgctcctg caacgcaccg ctgcgggtcc atgcctgcag
     8041 catttcgctg aaagagggca gatcaaagaa gaagtgttca gaatcacgca ttaccggggt
     8101 agcaccagaa accaccgatt tcggctcgat cagttcagtc gggctgtagg tcgcgccgca
     8161 gacttcgcag ttatcgccgt attgatccgg ggatttacat ttcgggcagg tgccttttac
     8221 aaaacggtcc ggcaggaaca tgcctttttc cggatcgtac agctgagaga tggtgcggtt
     8281 tttaataaaa ccgttttctt tcagacgaga gtagataagt tctgacaact ggcgattctc
     8341 ttcgctgtgc gtcgagtgat agttgtcata gctgatgtta aagcctgcga aatcagtctg
     8401 atgttcctga ctcatttcgc caatcatctg ctccggggtg ataccaagct gctgagcttt
     8461 aagcatgatc ggcgtgccgt gggcatcgtc ggcgcagatg aagttgacct cgtggccgcg
     8521 cattcgctgg taacggaccc agacatcagc ctggatgtgc tccagcatat ggccgaggtg
     8581 nnnnnnnnnn gaggtggatt gagccgttag cgtacggcag tgcgcacgtc accagaattt
     8641 tcttcgcgac ttgagtcata gtaggcatta cttcttaata gtgaaaaggg cttagatagt
     8701 accaaatggg aaaatgttaa gtaagtactt ctctatattc ttcaggtaaa ctatcagctg
     8761 atggtttatc tatacaaaaa aggagtcggg atgaacgaac aatcccaggc caaatcacca
     8821 gaagccttgc gcgcaatggt cgccgggaca ctggccaatt ttcagcaccc aaccctgaag
     8881 cataacctca caacgctgaa agcgttgcac catgtcgcct ggatggacga cacgctgcat
     8941 gtggaactgg ttatgccatt cgtctggcat agcgcgttcg aagagttaaa agagcaatgt
     9001 agcgccgaac tgctgcgcat caccggagcg aaagctatcg actggaaact gtcgcataac
     9061 atagccactc tgaaacgcgt gaagaatcag ccgggaatta acggcgtgaa gaacattatc
     9121 gcggtcagtt ccggtaaagg tggcgtgggt aaatcctcca cggcggtaaa cctggcgttg
     9181 gcactggcgg cggaaggggc gaaagtcggc attctggacg ccgatatcta cggcccatca
     9241 atccctacca tgctgggcgc agaaaatcag cgtccaactt cgccggacgg tacccatatg
     9301 gcaccgatta tgtctcatgg cctggcaact aattcaattg gttatctggt gacagacgac
     9361 aatgcgatgg tgtggcgtgg cccaatggcg agcaaggcgt taatgcaaat gttgcaggaa
     9421 accttgtggc cggatctcga ctatctggtg cttgatatgc cgccaggtac cggtgacatt
     9481 caattgacat tggcacaaaa cattccagta accggtgcag tcgtggtcac caccccgcag
     9541 gacatcgcgt tgatcgatgc gaaaaaaggc attgtgatgt tcgagaaagt cgaggttccg
     9601 gtgctcggta ttgtcgagaa catgagtgtg catatttgca gtaactgtgg tcaccatgaa
     9661 ccgattttcg gtaccggtgg tgcagagaaa ctggccgaga aataccacac acagctgctg
     9721 gggcagatgc cgttacacat ctccttacgt gaggatctcg ataaaggcac gccaacggtt
     9781 atcagccgtc ctgagagcga atttaccgct atctaccgcc agctggctga ccgcgttgca
     9841 gctcagctct actggcaggg tgaagtcatt cctggcgaga tttccttccg cgcggtctaa
     9901 cgcgtattaa ccattctggt tattcttcat acaccgcaga gatgttacat tgatgcggtg
     9961 tatttgtttt tgccgcatta atgaacactt atttaatgct gcgaatattc tgcggacctt
    10021 cgtccgaaac tctacaaagt cgattgatcg taccggcgat gcttcattat catccttcgt
    10081 ggctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
    10141 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
    10201 ctatggattg gcctctcccg cgttctcttc agaaactgcc acattaacga ttaatggtag
    10261 gatcagtccc ccgacatgca gtatggctat ggttaatggt caacctcaac agcattgtgg
    10321 tcagttaacg tataatgttg atacccgtca tcaggtttct tcgccagtta aaggtgtaac
    10381 gacggaagtg attgctgcag atagcgatag caaacgtcga atcgtgttaa atcgctacga
    10441 ttaatttgcc accctggttt taatcggatt taga
//
LOCUS       DAWDRC010000055        10430 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3265_length_10430_cov_2.049542, whole genome shotgun sequence.
ACCESSION   DAWDRC010000055 DAWDRC010000000
VERSION     DAWDRC010000055.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3265_length_10430_cov_2.049542"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1778..1787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2489..2498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4842..4851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5413..5422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7217..7260
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7473..7482
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8550..8559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2493 a         2879 c         2550 g         2404 t
ORIGIN      
        1 aaataggaaa accgcgacgg cagttcgggc aaaaaccaca gccagaaatg tgatacacca
       61 gcacgcggtc gccctcttta aaatggcggc agccttgccc catcgccaca atctgcccgc
      121 acggttcatg accgttgata aatccctggt ataacggttt atcgggtgcc gccgccgtag
      181 cacggtgctg gtggtagata tagtggacat cgcttccgca aatcccggag gatttcattt
      241 tgatcagtac ctggttaatc cccggcgtcg gcaccgcaac ttcccgcaga tcgacggtcg
      301 aatttcctgg taaataagct gccagcatcg ttttcataaa tccctcatta acaatacgat
      361 taattttcat ccctgccgca cccgcgccag gggcgttaag gtttagcgtt tcgctttact
      421 gctacgctgg gtcagcaaga tattcgccag caccgccacc acgatgatga cgccgcgcac
      481 cacctgctgg aaaaaggagt taatcccgag cagcaccaga ccgttaccga ttagcgtaat
      541 caccagtacg ccaagcagcg taccgaacaa cgagccgcga ccgccggaaa gcgccgtacc
      601 gccgacaacg accgcggcga tgacgtcaaa ctccagaccg tttgcggcac ctgcgttacc
      661 ggaaccgagg cgcgccgcca acaaaatgcc ggtcaccgcc gctaataatc ccgaaagggt
      721 aaaaataaga atgcgtaccc gacgaacgtt aataccgcac aactgcgctg cagtggcatt
      781 accgccaaca gcaaaaaccg agcgcccgaa ggcggttttg cggctaatga acacaaacag
      841 cgcaaataac accatcatga tcagcgcgga taccggcacc ccgagaaatt gtccgcccag
      901 ccagtccagc acttcgtttt cgttaattgg cactggcaag gcgttcgtca taaacagccc
      961 cattccgcgc agggcgctcc ataaacccag cgtcgcaaca aaacttggca cgttaaacac
     1021 gccgcgcagc accccggcga gcgttcccat cagcgcacct aacagcaaca ccagcagaca
     1081 agcaaacgcc agcggaacgt caaattgcag caaaaacgcc aggcacactg aaacaaaagc
     1141 caccatcggc ccaacgctga catcaatttc accggagata ataatcagcg tcatcgccca
     1201 ggcggcgatc ccaatggtgg cggcatcgcg cagcacgttc atctggttat tcaatgagat
     1261 aaagccaggc gcattcaggg agaagaccag ataaagaatg gcaatcacca ccagcaaacc
     1321 gatctcatta atatggcgac tgacaaattg tttgagcgag acgctcttgc cctgcggcaa
     1381 tggtaatgac gaagcagaca tcttcaggtt cctcggtaaa attcatcagt gcacagacag
     1441 aatggcggac atcagctcat ccacattgac cggagagtga aactcctgcg agaacgtacc
     1501 gtgctgtaac aacagaatgc ggtcgcacac caacggtaac tcctccactt cactggagat
     1561 aaacaccacg ctttttcctt cggcagccag ctcgcggaca atacggtaaa tctgctgttt
     1621 ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac aaaatctggc tggcggcata
     1681 gacccaacga ccgatcacca ctttttgctg attgccgcca gaaagcgtgc cgatgggcgt
     1741 ttcgctactg gcggccttga ccgtcatccg ctgcatcnnn nnnnnnnaac gtctcccgct
     1801 cgttcggcag acgcccctcg atcacctctt cggtcaggcg gcggatggtg gaccattgca
     1861 gcacaccgtt ggcgctgatt ttttgccgat tggtcagcac tgtattttcg tcaacgccca
     1921 accagggaat gatccccgct tctttgcggt tttctggcgt atagccaatg ccgcgtttca
     1981 gcatgtcgcc gtaatcgggg cgcgtgattt tctcgccgtt gataacaatt tcgccctgtt
     2041 catacgtctc cagcccaaca atcgccttca gcaattcact gcgccctgcc cccagcagac
     2101 cagcaatgcc gagcacttcg ccacgacgta gcgtaaaact gatatcctcc agcttgggct
     2161 tatggcgtaa cgcacggact tccagcacgg cttgatccat aatttcctga ggggcaaccg
     2221 gcgcaatatc aacgtgatcg cgcccgagca ttagcgacac aatatgatgc gtagaggtgt
     2281 tttcgagcat cacatcgccc gccacctgac catcgcgcat aacggtggca caggaggcaa
     2341 tgcggcgaat ttcttccatc cggtggctga cataaatcac cgccacgccc agtgccgaca
     2401 tctttttcac cgcgctgatc accagttcaa cttccgcact ggcaagcgag ctggtgggtt
     2461 catcaagaat gaccacgcgc ggctcgccnn nnnnnnnncc gttgcggcgg ggccactgac
     2521 cgaggcagag gttttccgcc actgtcagcc cttccaccag acttaactcc tgataaaccg
     2581 cacgaacccc cagttcagcg gcacggcgag tcagcgtagc ttcgtcacct tccagtcgcg
     2641 tctcgccaat ccagatatca ccgctatccg gacgttcgct accggtcagc attcgaatga
     2701 gggtcgattt tcccgcaccg tttttgccta acagcgcgcg aacttcgcct ttattgagcg
     2761 tgaagttaac gttatccagc gcaacgactc cgggataacg cttatttcct gccaccactt
     2821 ttgctaccgg gactgcctct gttgccgtga acatagggac ctctgcgaat cagcgattag
     2881 ggcagaccat caacgtgcgt tgccagccac tgtttaccgt cttccgtttt ggtatagaga
     2941 tcgattggca cctgaatcac tttttcaccg tcggcttgtt tattgataac cttcaatgtt
     3001 tgcgcgaaaa cagcattgcc cattttctta ccggaaatat ccaccaccgc tttcagcacc
     3061 tggttgtttt ccagctcctg agcaatttca gtggtcatat cagaaccgaa aacagcaatt
     3121 tttccggcct gattttgatt acgtaccgct tttaccgcgc cgagtgtcgc tccgcccgat
     3181 tcccccataa tggcgttgag atccggcgtg gagataatca gtttttcacc gacagaaatc
     3241 gctttatcta aaacagtccc ttcctgatta gcgacaattt gcgcgccggg aacgcgggtt
     3301 tttaatactt cttcaaaccc tttacgacgt tgcacacaaa cttcaaaggc ttcgcaattg
     3361 atgacggcaa ttttcggctg gtcaattttg ttggcaataa aataatcggc ggctgcgtta
     3421 cccagttttt taccaaattc cagcgggtca ccaaccagat atgccgagac atatttatcg
     3481 acaccctttt gattaataca ggtgttgtag caaatcaccg gaatacccgc ttcactggcg
     3541 cgacgaacgg tacggctact gccattttca gacactgccg ataaaataat ggcatcgaca
     3601 ttacgcgcca cgagggtatc aacaaaggta ctttctttcg aaatatcgcc ctgagcgtta
     3661 gtttcaatta actgtacctg tactgaagaa tcttttgccg catcctgaac gccctgacgc
     3721 actccggcgt aatatccctg ggtatcaagg tatattgcgc caatagtcat ttctttatca
     3781 gaagccctgg caaatagtgc gctacccaat agcgtagcca ttaataataa attacgggtt
     3841 cttgtcattt ttttaggcat agtgcctcct gtagggtttt tatttacaac ggcttatttt
     3901 aattatttga gcgtgagcgg cagcgcgtgc cgctcaaata ttacagttaa tagggtttag
     3961 tgaacgtagc taaaaataaa aggcatcacg gtggtgaata atgccgcttt cggccaggct
     4021 ggattaatcg ccatcaattc agcgcgagca gattcatagt gcgcaacctc ccctaatccc
     4081 gcggccgcca gcatcgccac catcaggcat ttttctttat gctgctgttg taaatcgcca
     4141 tacagcgaca acaagtcagg ctgcgagacg gcaaagaaat ccgcttcgat actggttttc
     4201 gccatctctt gtgcccactg tttcatttca ctaaacagtt gctgtgcggt gtgttgttcg
     4261 cccagcagtc gcagcgccat tccttgccag aagagataat caaccggctg atcgttgtaa
     4321 taactgtgga tgttaatggt gcgatcgccg gtcgccgcca gacgtaaaca acgcgtcgct
     4381 tcagtttcat cgccctgcgc gttggcgcat actgcctgcc agaaccagat gtcgttgtca
     4441 gtttgccccg gtaaacggcc ttcgcttaaa ttctccggat aatgcagcgc ggcatgaagc
     4501 agttcgctgg cctgctgcgg ctgtctggca tcaagatgct gccaggcgcg taataactgg
     4561 ttgaggataa actggctggt gaccttccct tccccacctt cccacgggtg gaatttgcgc
     4621 gtggcgagaa tgtccgccgt tttgtctgcc tgaccagtga gatgccacaa attgagcagt
     4681 tctgcggtca tgtcgtcgcg tttcagcgca atttccagat tattttccag acgcgccagt
     4741 cgtttctccg gtgtgacacc gcttaactta tccagcaaat ccagttcgaa aagcagacgt
     4801 gcatcctgcg gcgcaagctg ataagcatta tcaagataac gnnnnnnnnn nggccagctc
     4861 ataatcgtgc tgcttattcc acgcatggat cgccaacccg cgccagccgt cggcaaactc
     4921 cggcgacatc tctacgcaac gctgccataa ggcaatggct ttgccgtagc tacgcttgtt
     4981 gtagtagaag caggccagta aatgccgggc aaaccagcac tcttcgatac tctccagcgc
     5041 cgccacttct tccagcgtat tagggaaacg gacaaactgc gggaaggcat caatagcttt
     5101 tacgaccagt tctccgcgtt cagcttttgg tagcaaactg gcttgcaggt aaagcggtag
     5161 cgtgcgctga cagtccagtg cgttcagcat atctgccgcc agggcgggca tgccccagtt
     5221 aatcagttgc ccggcggtca tcagggcgtt aacgtcgcgt ccctgacaaa gacctcgcca
     5281 ctgcgcgagg gctgattcgc tacgaccatc gaaccagttc agccaccaca gagtggcgtt
     5341 cagcggataa tcacgcagca gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact
     5401 taacaccagt agnnnnnnnn nntgggcagg cgcgaagact ttgttggcaa aaatccagac
     5461 cagtgtcgaa gttaccatta cgcgccgcca gtcgtgccag accataatag ccgcccgctt
     5521 tactgttgcc gctccagacc gcacgccaga aatcctcttc ggcttgttga tattgcccct
     5581 gacgttcgta agcgctggcg cgaatcaaac tcgcctgtcc gcactgcgga tttttgttca
     5641 gcgcatgtgc gcgtttcagt gcctgactgg catataccac cgcttgcggg aaatctgcac
     5701 ggttatattc cagcattgcc agcgccagat tacagcgata atccagcgga tccagcgcca
     5761 cgccgcgcag atagtaatcg aacggtgaac ggctggcgtg atgatattgc tccagatgtt
     5821 gaccgataaa ccaggcttca tctgtactgg taatgtcttg tgctgccagt ggcgctttgg
     5881 cgacgtccgg caacggcaac gcttgcggct gatgttcctg ataactcagt acaatattgc
     5941 cgtcggcatc ggagagctca atggtcagcc tttcaggatt gataccgtgc aacacgccct
     6001 ggatggcggt cgctggtgtc aggaccaccg catcatcgag caacgcgttg catttgccga
     6061 tttcgcggat cgccagacgg tatccgttca acggagagat ggcatacagc ccccactcaa
     6121 tcccccgctc gctacgctgg agtttaatca ccgcatcgcg ggaggcgttt tgcaccatgc
     6181 ccaaagaatg gtaaggcagg aaatactgct cgaaacgctt ctcctcgtaa gcatcaagcc
     6241 aggtaaaatc aggctggtta tcggcaaaaa taccggtcat cagttcgata tacgggccgt
     6301 tattgtcagt caggctctta tcccacgcct ggccaaattc actgtgtccc cagctccact
     6361 gttttttacc tggcgcaata tggtggttgg caacgtgtag caaaccacca tcttcatcgt
     6421 gacaccacgc gccaacaaaa tcgtactgtg atttttcagc catatatgag gttggaacgg
     6481 gcacattttt atagcgagaa atgtccaccc cggcggagta gtccactttg tagtaagtgc
     6541 cggtggcgat ggggaaagcg gagacggccc gtttgccgtg atcaaacacc gcagtcacat
     6601 ccggtgggaa aacgctctga tgcccttcac cccctttcac tgccgggttg gcccaccaca
     6661 agaaatgacg cggcgtggcg ttgccgttat agacgcgact ggcgatttcc agcgccgccc
     6721 ggtcagggcg cagggtgaaa cctgtcatca cctgtaaacc gtgcatcggc tccgtttcgc
     6781 cgacccacac cgtctgtgcg ccgtcgtcat gggcttcgag ggtgaaatca acgggcataa
     6841 aggtggtcgg gcgatggtgt tgcggccagt taaactcaat cccgccggag atccacggcc
     6901 ccagcagtcc caccagcgcc ggtttaatga cttcattgtg ataaacaaaa tcgcgctgtt
     6961 tcactttatc ccacgcgcga tgcacccgac cgcccagttc cggcaggatc atcactttga
     7021 tgtagtcgtt ttccagccac accgcctgcc aggatttcag ggttttctgc tcgctcagcg
     7081 tatcggtcac gccgtaggga taaaccgcgc cggacgatcc ctgataaacg cgattttcca
     7141 ggaacatggg atgtatatcc tgcggcccgg tttcataggt cgggatctca acgcgctctt
     7201 gcccaacttt tactggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 aacaagacat actgaattaa aagatttgtg gcagtgtatt gaacaatctg gcactgtttt
     7321 cgcggaataa tcacgcaatt aactaaacag ggtttagtga agatgagagc ctgcattaac
     7381 aatcaacaga ttcgccacca taacaaatgc gtgattctgg aactgctgta ccggcaaaag
     7441 cgcgccaata aatcaacgct ggcccggctg gcnnnnnnnn nnagcgaaaa acgggtggtg
     7501 aatatcgacg atgaaagtca aacgcgcgga catagtagcg gtacatggct gattgcgccg
     7561 gaaggtgact ggacgctgtg tctgaacgtg acgcccacca gcattgagtg tcaggtcgct
     7621 aatgcctgtt taagtccgaa aggcgaattt gaacgctttc agattgacgc tccaacgccg
     7681 caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt gtggccggac
     7741 cgcaccatca atctggctct ggcaatccac ggtcaggtgg atccggtgac cggcgtgtcg
     7801 caaaccatgc cacaagcgcc gtgggcaaca ccgattgagg tgaagtacct gctggaagag
     7861 aagctgggca ttcgggtaat ggtcgataat gactgcgtga tgctggcgct ggcggagaaa
     7921 tggcaaaata attcgcagga acgggatttc tgcgtaatca acgttgatta cggcattggc
     7981 tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt tgtatggcag tggacagatt
     8041 ggtcacacca tcgttaatcc ggatggcgtc gtctgcgact gcgggcgtta tggctgcctg
     8101 gaaaccgtcg cctcgttaag cgcattaaaa aaacaggcgc gggtatggct aaatcacaac
     8161 cggttaatac tcagcttgat cctgaaaaac tgactaccgc gcagttaatc gctgcctggc
     8221 agagtggaga accgtggatc accagttggg ttgatcgctc tgccaacgcc attggtttga
     8281 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
     8341 gtgcctttgg tgagaactgg cttaatacca ttattcgcca gacgggattt aacccgttcg
     8401 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg ctttgggcaa ttaagccgcg
     8461 cacagcaggt gctgggaatt ggctatttgt atgttgaggc gcagttacgg cagatttgat
     8521 ggcgcaataa cgtagaaagg cttcccgaan nnnnnnnnnt caacagatta accgtttcgc
     8581 ttatgcgtaa accggataac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat
     8641 aacagcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc
     8701 ttcggcttct ttaaagccac gacgggtaat cgccggagta cctacacgaa taccggaggt
     8761 cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg
     8821 gcccagggcg gcgtcagctt ctttaccggt caggttttta tcaaccagat caaccaggaa
     8881 caggtggtta tcagtgccgc cggaaaccac tttgtagccg cgttccagca acacttctac
     8941 catcgcttta gcgtttttcg caacctgctg ttggtaagtt ttgaactcag gctccatcgc
     9001 ttctttcaga gcaaccgctt taccagcgat tacgtgcatc aacgggccgc cctgaccacc
     9061 agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat
     9121 caggccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg
     9181 aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac
     9241 gaacaggtaa gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt caaccacgcc
     9301 ggaatatgca gagaggccac cgataatcat tttcggtttg tgttctttgg cttgtttttc
     9361 cagatcggcg tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag
     9421 tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccagattcat
     9481 acccagaacg gtatcgcctg gttccagcaa cgcggtgtag accgcaaagt tagcctggga
     9541 gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat
     9601 cgccagttgt tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata
     9661 accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt
     9721 gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat
     9781 agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat
     9841 ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg
     9901 cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac
     9961 gattaccttc aggctaagca aggctttgga gaataaagag cttgcaaccg gaaacggatt
    10021 tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa
    10081 agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag
    10141 atgcaaaaaa aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc
    10201 atccctttac tggtggaaac gggaccaaag ttaaccgccc atttctacga ccgtatgttt
    10261 actcataacc cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa
    10321 cgtgaagccc tgtttaacgc tattgccgcc tacgccagca atattgaaaa cctgcctgcg
    10381 ctgctgccag cggtagaaaa aattgcgcag aagcacacca gtttccagat
//
LOCUS       DAWDRC010000056        10289 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3315_length_10289_cov_2.587258, whole genome shotgun sequence.
ACCESSION   DAWDRC010000056 DAWDRC010000000
VERSION     DAWDRC010000056.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10289)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3315_length_10289_cov_2.587258"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    8045..8054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2596 a         2488 c         2674 g         2521 t
ORIGIN      
        1 aaattaattt cacatcacct ccgctatatg taaagctaac gtttctgtag ctcgacgcaa
       61 cttcctcatt cttctctcca aaaaccacct catgcaatat aaaaaactgc aaataaagat
      121 aactatagaa cattaagcca acaaataaac tgaaaaagtt tgtgcgcgat gctttcctct
      181 atgagtcaaa atggccccaa atgtttcatc ttttgggggg aaactgtgca gtgttggcag
      241 tcaaactcgt tgacaaaaca aagcgtacag aacgactgcc catgtcgatt tagaaatagt
      301 ttttttaaag gaaagcagca tgaaaattaa aactctggca atcgttgttc tgtcggctct
      361 gtccctcagt tctacagcgg ctctggccgc tgccacgacg gttaatggtg ggaccgttca
      421 ctttaaaggg gaagttgtta acgccgcttg cgcagttgat gcaggctctg ttgatcaaac
      481 cgttcagtta ggacaggttc gtaccgcatc gctggcacag gacggagcaa ccagttctgc
      541 tgtcggtttt aacattcagc tgaatgattg cgataccaat gttgcatcaa aagccgctgt
      601 tgccttttta ggtacggcga ttgatgcgac tcataccaac gttctggctc tgcagagttc
      661 tgctgcaggt agtgcaacaa acgttggtgt gcagatcctg gacagaacgg gtgctgcgct
      721 gacgctggac ggtgcgacat tcagtgagca aacaaccctg aataacggta ctaacaccat
      781 tccgttccag gcgcgttatt atgcaaccgg tgtcgcaacc ccgggtgctg ctaatgcgga
      841 tgcgaccttc aaggttcagt atcaataacc tacccaggtt cagggacgtc attacgggca
      901 gggatgccca cccttgtgcg ataaaaataa cgatgaaaag gaagagatta tttctgttag
      961 cgtcgttgct gccaatgttt gctctggccg gaaataaatg gaataccacg ctgcccggcg
     1021 gaaatatgca atttcagggc gtcattattg cggaagcttg ccggattgaa gccggtgata
     1081 aacaaatgac ggtcaatatg gggcaaatca gcagtaaccg gtttcatgcg gtaggggaag
     1141 atagctcacc ggtgcctttt gttattcatt tacgggaatg tagcacggtg gtgagtgaac
     1201 gtgtgggtgt ggcgtttcac ggtgtcgcgg atggtaaaaa tccggatgtg ctttccgtgg
     1261 gagaggggcc agggatagcc accaatattg gtgtagcgtt gtttgatgat gaaggaaacc
     1321 tcgtaccgat taaccgtcct ccagcaaact ggaaacggct ttactcaggc tctacttcgc
     1381 tacatttcat cgccaaatat cgtgctaccg ggcgtcgggt tactggcggc atcgccaatg
     1441 cccaggcctg gttctcttta acctatcagt aattgttcag cagataatgt gataacagga
     1501 acaggacagt gagtaataaa aacgtcaatg taaggaaatc gcaggaaata acattctgct
     1561 tgctggcagg tatcctgatg ttcatggcaa tgatggttgc cggacgcgct gaagcgggag
     1621 tggccttagg tgcgactcgc gtaatttatc cggcagggca aaaacaagtg caacttgccg
     1681 tgacaaataa tgatgaaaat agcacctatt taattcaatc atgggtggaa aatgccgatg
     1741 gtgtaaagga tggtcgtttt atcgtgacgc ctcctctgtt tgcgatgaag ggaaaaaaag
     1801 agaatacctt gcgtattctt gatgcaacaa ataaccaatt gccacaggat cgggaaagtt
     1861 tattctggat gaatgttaaa gcgattccgt caatggataa atcaaaattg actgagaata
     1921 cgctacagct cgcaattatc agccgcatta aactgtacta tcgtccggct aaattagcgt
     1981 tgccacccga tcaggccgca gaaaaattaa gatttcgtcg tagcgcgaat tctctgacgc
     2041 tgattaaccc gacaccctat tacctgacgg taacagagtt gaatgccgga actcgggttc
     2101 ttgaaaatgc attagtgcct ccaatgggcg aaagcgcggt taaattgcct tctgatgcag
     2161 gaagcaatat tacttaccga acaataaatg attatggcgc acttaccccc aaaatgacgg
     2221 gcgtaatgga ataacgcagg gggatttttt cgcctgaata aaaagaattg actgccgggt
     2281 gatttaaacc ggaggaataa tgtcatatct gaatttaaga ctttaccagc gaaacacaca
     2341 gtgcttgcat attcgtaagc atcgtttggc tggttttttt gtccgactct ttgttgcctg
     2401 tgcttttgcc gcacaggcac ctttgtcatc tgccgaactc tattttaacc cgcgtttttt
     2461 agcggatgat ccccaggctg tggctgattt atcgcgtttt gaaaatgggc aagaattacc
     2521 gccagggacg tatcgtgtcg atatctattt gaataatggt tatatggcaa cgcgtgatgt
     2581 ctcatttaat acgggcgaca gcgaacaagg gattgttccc tgcctgacac gcgcgcaact
     2641 tgccagtatg gggctgaata cggcttctgt ctccggtatg aatctgctgg cggatgatgc
     2701 ctgcgtgcca ttaacctcaa tgatccatga cgctactgcg catttagatg ttggtcagca
     2761 gcgactcaac ctgacgatcc ctcaggcatt tatgagtaat cgcgcgcgtg gttatattcc
     2821 tcctgagtta tgggatcccg gtattaatgc cggattgctc aattataatt tcagcggaaa
     2881 tagtgtacag aatcgaattg ggggtaacag ccattatgca tatttaaacc tacagagtgg
     2941 gttaaatatt ggtgcgtggc gtttacgcga caataccacc tggagttata acagtagcga
     3001 cagttcatca ggtagcaaaa ataaatggca gcatatcaat acctggcttg agcgagacat
     3061 aattccgtta cgttcccggc tgacgctggg tgatggttat actcagggcg atattttcga
     3121 tggtattaac tttcgcggcg cacaattggc ctcagatgac aatatgttac ccgatagcca
     3181 aagaggattt gccccggtga tccacggtat tgctcgtggt actgcacagg tcactattga
     3241 acaaaatggg tatgacattt ataatagtac ggtgccaccg gggcctttta ccatcaacga
     3301 tatctatgcc gcaggtaata gtggtgactt gcaggtaacg attaaagagg ctgacggcag
     3361 tacgcagatt tttaccgtac cctattcgtc agtcccgctt ttgcagcgtg aagggcatac
     3421 acgttattcc attacggcag gagaataccg tagtggaaat gcacaacagg aaaaaccccg
     3481 ctttttccag agtacattac tccacggcct tccggctggc tggacaatat atggtggaac
     3541 gcaactggcg gatcgttatc gtgcgtttaa tttcggtatc gggaaaaaca tgggggcgct
     3601 gggtgctctg tctgtggata tgacgcaggc taattccaca cttcccgatg acagtcagca
     3661 tgacggacaa tcggtgcgtt ttctttataa caaatcgctc aatgagtcag gtacgaatat
     3721 tcagttagtg ggttaccgtt attcgaccag tgggtatttt aatttcgctg atacaacata
     3781 cagtcgaatg aatggctaca acatcgaaac acaggatgga gttattcagg ttaagccgaa
     3841 attcaccgac tattacaatc tcgcttataa caaacgcggg aaattacaac tcaccgttac
     3901 tcagcaactc gggcgcacat caacactgta tttgagtggt agccatcaaa cttattgggg
     3961 aacgagtaat gtcgatgagc aattccaggc tggattaaat actgcgttcg aagatattaa
     4021 ctggacgctc agctatagcc tgacgaaaaa cgcctggcaa aaaggacggg atcagatgtt
     4081 agctcttaac gtcaatatac ctttcagcca ttggctgcgt tctgacagta aatctcagtg
     4141 gcgacatgcc agtgccagct acagcatgtc acacgatctc aacggtcgga tgaccaatct
     4201 ggccggtgta tacggtacgt tgctggaaga caacaacctc agctatagcg tgcaaaccgg
     4261 ttatgccggg ggaggcgatg gaaatagcgg aagtacaggc tacgccacgc tgaattatcg
     4321 cggtggttac ggcaatgcca atatcggtta cagccatagc gatgatatta agcagctcta
     4381 ttacggagtc agcggtgggg tactggctca tgccaatggc gtaacgctgg ggcagccgtt
     4441 aaacgatacg gtggtgcttg ttaaagcgcc tggcgcaaaa gatgcaaaag tcgaaaacca
     4501 gacaggagtg cgtaccgact ggcggggtta tgccgtgctg ccttatgcca ctgaatatcg
     4561 ggaaaacaga gtggcgctgg ataccaatac cctggctgat aacgtcgatt tagataatgc
     4621 ggtcgctaac gttgttccca cccgtggggc gatcgtgcga gcagagttta aagcgcgcgt
     4681 tgggataaaa ctgctcatga cgctaaccca caataataag ccgctgccgt ttggggcgat
     4741 ggtgacatca gagagtagcc agagtagcgg cattgttgcg gataatggtc aggtttacct
     4801 tagcggaatg cctttagcgg gaaaagttca ggtgaaatgg ggagaagagg aaaatgctca
     4861 ctgtgtcgcc aattatcaac tgccaccaga gagtcagcag cagttattaa cccagctatc
     4921 agctgaatgt cgttaagggg gcgtgatgag aaacaaacct ttttatcttc tttgcgcttt
     4981 tttgtggctg gcggtaagtc acgctttggc tgcggatagc acgattacta tccgcggcta
     5041 tgtcagagat aatggctgta gtgtggccgc tgaatcaacc aattttactg ttgatctgat
     5101 ggaaaacgcg gcgaagcaat ttaacaacat tggcgcgacg actcctgtcg ttccatttcg
     5161 tattttgctg tcaccctgtg gtaacgctgt ttctgccgta aaggttgggt ttactggcgt
     5221 tgcagatagc cacaatgcca acctgcttgc acttgaaaat acggtgtcag cggctgcggg
     5281 actgggaata cagcttctga atgagcagca aaatgagata ccccttaatg ccccatcgtc
     5341 cgcgatttcg tggacgaccc tgacgccggg taaaccaaat acgttgaatt tttacgcccg
     5401 gctaatggcg acacaggtgc ctgtcactgc ggggcatatc aatgccacgg ctaccttcac
     5461 tcttgaatat cagtaactgg agatgctcat gaaatggcgc aaacgtgggt atttattggc
     5521 ggcaatgttg gcgttcgcaa gtgcgacgat acaggcagcc gatgtcacca tcacggttaa
     5581 cggtaaggtc gtcgccaaac cgtgtacggt ttccaccacc aatgccacgg tagatctcgg
     5641 cgatctttat tctttcagtc tgatgtctgc cggggcggca tcggcctggc atgatgttgc
     5701 gcttgagttg actaattgtc cggtgggaac gtcgagggtc actgccagct tcagcggggc
     5761 agccgacagt accggatatt ataaaaacca ggggaccgcg caaaacatcc agttagagct
     5821 acaggatgac agtggcaaca cattgaatac tggcgcaacc aaaacagttc aggtggatga
     5881 ttcctcacaa tcagcgcact tcccgttaca ggtcagagca ttgacggtaa atggcggagc
     5941 cactcaggga accattcagg cagtgattag catcacctat acctacagct gaacccaaag
     6001 agatgattgt aatgaaacga gttattaccc tgtttgctgt actgctgatg ggctggtcgg
     6061 taaatgcctg gtcattcgcc tgtaaaaccg ccaatggtac cgctatccct attggcggtg
     6121 gcagcgccaa tgtttatgta aaccttgcgc ctgctgtgaa tgtggggcaa aacctggtcg
     6181 tggatctttc gacgcaaatc ttttgccata acgattaccc ggaaaccatt acagattatg
     6241 tcacactgca acgaggctcg gcttatggcg gcgtgttatc taatttttcc gggaccgtaa
     6301 aatatagtgg cagtagctat ccatttccta ccaccagtga aacgccgcgg gttgtttata
     6361 attcgagaac ggataagccg tggccggtgg cgctttattt gacgcctgtg agcagtgcgg
     6421 gcggggtggc gattaaagct ggctcattaa ttgccatgct tattttgcga cagaccaaca
     6481 actataacag cgatgatttc cagtttgtgt ggaatattta cgccaataat gatgtggtgg
     6541 tgcccactgg cggctgtgat gtttctgctc gtgatgtcac cgttactctg ccggactacc
     6601 ctggttcagt gccgattcct cttaccgttt attgtgcgaa aagccaaaac ctggggtatt
     6661 acctctccgg cacaaccgca gatgcgggca actcgatttt caccaatacc gcgtcgtttt
     6721 cacccgcgca gggcgtcggc gtacagttga cgcgcaacgg tacgattatt ccagcgaata
     6781 acacggtatc gttaggagca gtagggactt cggcggtaag tctgggatta acggcaaatt
     6841 acgcacgtac aggagggcag gtgactgcag ggaatgtgca atcgattatt ggcgtgacat
     6901 ttgtttatca ataaagaaat cacaggacat tgctaatgct ggtccctgag gcgctacgct
     6961 atcagaccta caaaacctgc acgttagccc tttgtaggcc agatgcgcca gcgtcgcatc
     7021 tggcataaac aaagcgcact ttgccgacaa tccgaacaga gcctgccatt ggcaggctcc
     7081 ggtgttcttt tacgctacca tgctaataat caacacaata atcagcccaa ccacggagtt
     7141 gaccagctcc agcagacccc aggttttcaa cgtgtctttt actgacaggt caaagtaatc
     7201 taagaggcat tgctaatgta gggaatgtgt ctgaacctgc ggtcattgtc agtaccagca
     7261 tcaggccaat gccgaatacc acccagagaa tgttaagcac atgcataacg ttttacctta
     7321 cctggttgaa ccgttgttat tttgggcgac atgttatgta aattggtcaa ccattgttgc
     7381 gatgaatgtc acatcctctg attaataacc atcgattacc ctttgctgca atttgcagca
     7441 acaaccagga gagtgaaatt cttgtgatgt ggttaaccaa ttttagaatt cgggttgaca
     7501 tgtcttacca aaaggtagaa cttatacgcc atctcatccg atgcgacgct acggctgtgg
     7561 ccgggttgtt catccggata cctaaacaac tccggggttc cgcgtctctt cgctgttgaa
     7621 cccactatgt gaaagaggaa aaatcatgga acagacctgg cgctggtacg gcccaaacga
     7681 tccggtttct ttagctgatg tccgtcaggc gggcgcaact ggcgtggtta ccgcgctgca
     7741 ccatatcccg aatggcgaag tatggtccgt tgaagagatc ctcaaacgca aggcgatcgt
     7801 tgaagacgca ggcctggtat ggtctgtcgt agaaagcgtg ccaattcacg aagatatcaa
     7861 aacccacacc ggcaactatg agcagtggat cgctaactat cagcagactc tgcgcaacct
     7921 ggcgcagtgc ggcattcgca ccgtgtgcta caacttcatg ccggtgctcg actggacccg
     7981 tactgacctc gaatacgtgc tgccagacgg ctccaaagcg ctgcgtttcg aacagatcga
     8041 atttnnnnnn nnnncacctg gagctgtaca gagatatcga caaagccaaa ctgcgcgaaa
     8101 actttgctgt cttcctgaaa gccattattc cggttgccga agaagttggc gtccgtatgg
     8161 cggttcaccc ggacgatccg ccgcgcccga tcctcggcct gccgcgcatt gtttccacca
     8221 ttgaagatat gcagtggatg gttgataccg taaacagcat ggcgaacggt ttcaccatgt
     8281 gcaccggttc ctacggcgtg cgtgctgaca acgatctggt tgatatgatc aagcagttcg
     8341 gtccgcgtat ttacttcacc catctgcgct ccaccatgcg tgaagataac ccgaaaacct
     8401 tccacgaagc ggcgcacctg aacggtgacg ttgatatgta cgaagtggtg aaagcgattg
     8461 ttgaagaaga acaccgtcgt aaagcggaag gcaaagaaga cctgatcccg atgcgtccgg
     8521 accacggtca tcagatgctg gacgacctga agaagaaaac caacccaggt tactccgcaa
     8581 ttggtcgtct gaaaggcctg gccgaagttc gcggtgtcga actggcgatc cagcgcgctt
     8641 tctttagccg ttaatatcca ccggcatggc tgcgcgccgt gccggttcct tcttccttgc
     8701 cgtcactctc tgaagacgga ttctggagtt tacgatgact actattgttg acagcaatct
     8761 gccggttgcc cgcccgtcat gggatcattc tcgtctggaa tcacgcattg tgcatctcgg
     8821 ttgcggggcg tttcaccgcg cgcaccaggc gctgtatacc catcatctgc tggaaagtac
     8881 cgacagcgac tggggtatct gcgaagttaa cctgatgcca ggcaacgacc gcgtgctgat
     8941 cgaaaacctg aaaaaacagc aactgctgta caccgtggcg gaaaaaggcg cggaaagcac
     9001 cgagctgaaa attatcggtt cgatgaaaga agcgctgcat ccggaaatcg acggttgcga
     9061 aggtattctc aacgcgatgg cgcgtccgca gacggcgatt gtctctctga cggtcacgga
     9121 aaaaggctac tgcgctgatg cggcaagcgg tcagctggat ctcaataacc cgctgatcaa
     9181 gcacgatctc gaaaacccga ctgcgccgaa gtctgcgatt ggttacatcg tagaagccct
     9241 gcggctgcgt cgtgaaaaag ggctgaaagc gtttacggtg atgtcctgcg ataacgtgcg
     9301 tgaaaacggt catgtggcga aggttgccgt tcttgggctg gctcaggcgc gtgacccgca
     9361 actggcggca tggattgaag aaaacgtcac cttcccgtgc accatggttg accgcatcgt
     9421 tccggcggcg acgccagaaa ccttacagga aattgctgac cagctgggtg tttacgaccc
     9481 gtgcgccatt gcctgcgaac cgttccgtca gtgggtgatt gaagataact tcgttaatgg
     9541 tcgtccggac tgggataaag tcggcgcgca gtttgttgcg gatgttgtac cgttcgaaat
     9601 gatgaagctg cgtatgctga acggtagcca ctctttcctg gcgtacctcg gttacctcgg
     9661 cggctatgaa actattgccg acaccatgac taacccggct tatcgcaaag cggcctttgc
     9721 cctgatgatg caggaacaag cgccaacgct gtcgatgccg gaaggtacag acctgaacgc
     9781 ctatgcgacg ctgctgatcg agcgtttcag caacccgtct ctgcgtcacc gcacctggca
     9841 gattgcgatg gacggcagcc agaagttacc gcagcgtctg ctggatccgg tgcgtctgca
     9901 cctgcaaaac ggcggcagtt atctgctgct aaccgttagg cagaaaaata gccaggtcag
     9961 cttactgtac ctggatgcac tcggcgtccc ccttgcggat ggacagctca tagccccgga
    10021 ccgtgacctc caccgggtcg cccagggggg ccacctttcg gacataaata gatgtcccct
    10081 tggtgatgcc catgtccatg atgcggcggc gcagggcgcc ctcgccgttg agcttgacca
    10141 cagtgacggt ctgccccacc tttgcctcct tcaatgtgct catctagact ctcctccttc
    10201 tccggccgga ctcataccag gatgcgtccg gccatattct tgctcaaggc gatccgcgct
    10261 tccttgatgt tgacgatcag gttaccgcc
//
LOCUS       DAWDRC010000057        10257 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3327_length_10257_cov_2.241031, whole genome shotgun sequence.
ACCESSION   DAWDRC010000057 DAWDRC010000000
VERSION     DAWDRC010000057.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_10257_cov_2.241031"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1337..1346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2538..2547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5699..5708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6865..6874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7642..7651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2807 a         2148 c         2394 g         2858 t
ORIGIN      
        1 caactgtaac aaggggccgg ttaggtgagg gattatctcc gttcattagt catcccattt
       61 gtggctgaaa tacgcggcca gaaacccaga aaataaaatt attcccagta cggccataaa
      121 aacattcata ttacccagca ttatttgcct ccgtttatgg tgtggtgtgt ttcctcgctt
      181 accttaattt tcatctcaat gagaaagata ttagcagagg taacctaaac gaaggctgaa
      241 ttaaattctt attgtgttcg acttaaatca acaaagcgcg ggctgccccc acggtcttgc
      301 acgaattcct tttaacatca gcacccacgc aacaacgatc accaacgcta agatgataaa
      361 cagcgagaaa ggcggcagac tggtaagtac tatgccactc actaatgggt taatcgcagc
      421 accaagccag cctaaagact gggcggaaaa atagctggct ttcattcctg gcggcgcaat
      481 atggtaaatc aacatatatt cgcccggcgc ataaatgatt tcaccgacag taaataccgc
      541 agctgacata ccccacaata gcaggctgtt gccggaaaaa ataaaaccga ccagcccgat
      601 gacgaaacag agggtgcctg ctgtcatcag cgcgcggatg ttagccgggt taaggcggcg
      661 gccaacggaa tattgcaacg taaccaccat ggcagcattc accggaagaa caaccgcgac
      721 caccttttcg gcaaaatcac catcagcaat caccatcaca tattgtgaaa tgcatgaagc
      781 aaatgcgccg ctaacaaaag aagccagaaa gccagagcag gtaaaccaca acagtgcttt
      841 atcttgtaat aaaactttcg gcgaccagac actgcctgtt tccgtggcga tgattttctc
      901 gctgcgcttt acccaaattt gaatgaaaag catgggaaac gcggaacaga tagctgccag
      961 ccagaagggc agattgatgc tctgcatcac caacagcgtg ccgagtggcg gaccgatggt
     1021 ccagccaatg ttgagcatgg tgtagttgat tgagaaaatc ttcgttttgc tggtggacga
     1081 aagattgtcg gcaaaccagg ctttcagcac ggtagcaaaa acggagtagg cgcagttaat
     1141 aagggcaaaa aagagcacca ccagcgtcac gttattcact aaaggaatgg caataaaacc
     1201 gctagcgaag gcggtaattg ccagtaacat atagcgtttc ttgtcgaact tatccgccag
     1261 gataccaaaa ccgaggctaa aaacgacgcc aatagtgagc gcaattgtca tcgcataacc
     1321 gattagatcg acactcnnnn nnnnnntttt gttttagaga tgtaaaggaa tagcatggca
     1381 aaaatccgaa gtatagcgac ttatttgcgc attaatgccg tcgtaaacgg tgactaacga
     1441 atcaactgtt aaagaaaagt aaattcagct aagttgattg cttacaaaag atgaaattca
     1501 gaggcggtag aatgtcgcga aaagatgggg tgttggcgct actggtagtg gttgtatggg
     1561 ggctaaattt tgtggtcatc aaagtggggc ttcataacat gccaccgctg atgctggccg
     1621 gtttgcgctt tatgctggtc gctttcccgg ccatcttttt tgtcgcacga ccgaaagtac
     1681 cactgaattt gctgctgggg tatggattaa ccatcagttt tgcgcagttt gcttttctgt
     1741 tctgcgccat taactttggt atgcctgctg gactggcttc gctggtgtta caggcacagg
     1801 cgttttttac tatcgtgctt ggcgcgttta ctttcggtga acgactgcat ggcaaacaat
     1861 tggcggggat cgccttagcg atttttggcg tactggtgtt aatcgaagat agtctgaacg
     1921 gtcagcatgt ggcgatgctc ggctttatgt tgaccctggc ggcagcattt agttgggcgt
     1981 gtggcaacat cttcaataaa aagatcatgt cgcacgcaac gcgtccggca gtgatgtcgc
     2041 tggtaatctg gagcgcatta atcccaatca ttcccttctt tgttgcctcg ctgattctcg
     2101 atggttccgc aaccatgatt cacagtctgg ttactatcga tatgaccacc atcttgtctc
     2161 tgatgtatct ggcgtttgtg gcgacaattg ttggttatgg gatctggggg acgttactgg
     2221 gacgctatga aacctggcgg gttgcaccgt tatcgttact ggtgcctgta gttggactgg
     2281 caagtgcggc actattgctg gatgaacgct taacgggtct gcaatttttc ggtgcggtgc
     2341 tcattatgac cgggctgtat atcaatgtat ttggcttgcg gtggcgtaaa gcggtaaagg
     2401 tcagaggcta aaccatacgc acagaatgtg atgccagcac tctgtgcgtc tgcttttatc
     2461 gcgggaaaaa gtactttacc tggaactcga aaatattgct gtaaagtaac gaataatcca
     2521 gcggaataga gttactannn nnnnnnncgt tctcaatata cgaaaatttg gatttttgca
     2581 tatgcgatac ggttaaatca ggaaaacgtg ccactgacat ccacgtttct tctaattgtg
     2641 ctgcattatc ctcaatgaat cgcagtctct ttatgtaata aacctgctca ccagtcacta
     2701 aattcagctc agtcgcaata gtaggagaag catcctgcac ttcaaatttc ataatctgac
     2761 ttcctgcctc tttgccgctt ttatgtgcaa tttcactgaa actgttcatg tgatcaatgt
     2821 gtgcaacaaa attctttttt tgaatgtatg ttcctgaacc atgctttctg tgaataattc
     2881 cttcgccttc cagcagggat aatgccttac gcagggtatt acgcgaaaca ttataacgaa
     2941 cagcgagttc cgcttctgac ggcaacaaat caccaacata ccatgaattg ccattgatct
     3001 cggatttaag taagcgggca atcttttgaa aaatcacttt tgcacctttt aagagccatt
     3061 gaaaacaagg agttgagacc tgattagttt atcgtatggc cgagtcgttt aattatcacc
     3121 ctgttgttga acaactttat tgctgtatta ctgtgatcaa tgtaccagga cgtgtagcta
     3181 ctgtgggata tgatatttac acaatgtagt ctgttatata ccagtaagga aaaatatgaa
     3241 aaagatattg ttagtttgcg ctgcgggcat gtcaaccagt atgctggtta aacgtatgat
     3301 tgatcatgct accgctattt cacttgaagt taatatttcc gcattggcga ttgcagaggc
     3361 taaagggaaa attaaaaata acgaagttga cgttgtttta cttggtccgc aagttcgctt
     3421 tcagaaacca gagattgaag ccgttgcaca ggggaaaatg cctgtagccg ttatcgagat
     3481 gaaagactat ggcacaatga acggacaggc agttcttgaa tttgcgatga aactactgca
     3541 agagtaagaa tctaatatta ttaaactatt acctgaacta acttttcaca catcataaaa
     3601 tggatatggc atacatgctt tattgggatg tataaatatc caggacaaca ggtataaaaa
     3661 aatgggatta atggcatcat tcgaacgtgg aatggaacgt tttcttgttc cagttgctat
     3721 caagttaaac tcacaaaaac atgttgcagc ggtgagagat ggattcgttt ttacgtttcc
     3781 aattatcatg gcaagctcat taattatatt aattaacttt gccatattat cgcccgacgg
     3841 ttttattgcc ggattactgc atctgaacag cattttcccc aaccttgaaa aagcacaagc
     3901 tatttttact ccggtaatga atggttctgt aaatatcatg tcaattatga ttgctttcct
     3961 ggtcgccagg aatgtggcga ttagctatga gcaagatgat cttttatgcg gattaacggc
     4021 aataggagca ttttttattg tttatacccc ataccagatg atagatgggc aggcattcct
     4081 gacgaccaaa tatctcggcg cgcaggggtt gtttgttgct gttatcgttg cattgatcac
     4141 cagtgaaata ttttgtcgct tagctcgaaa ccccaaaatc accataacga tgccggcagc
     4201 tgtacctcct gcggtagcgc gttcatttaa agttttattg ccaatatttt ttgtcatggt
     4261 gttcttttcc gcacttaatt attgcctgac attgatatcc ccggcaggat taaatgatct
     4321 catttacaca ttaatccaga cgccgctcaa acatatggga acgaatatct ttgcggtaat
     4381 tatcctgggg gctgtgggta atttcctctg ggtgctgggg atccacggac ctaatacaac
     4441 gtcggcaatt cgagaaaccg ttttttctga ggctaatctg gagaatctct cctgggccgc
     4501 tcaacacggc actacctggg gcgcgccata tccgattacc tggacttcta ttaatgatgc
     4561 attcgccaac tgcggcggtt caggtatgac gttggggtta ttgttggcta tttttatcgc
     4621 ttctaagcgt gcggaatacc gtgatctggc aaaaatgtca tttatccccg gtattttcaa
     4681 tatcaatgaa ccgataatgt tcggccttcc tattgtactt aaccccatca tgatggtgcc
     4741 gtttattatg gttcccattg ttaactgtgc cattggttac ttctttgttt cgatggaaat
     4801 tattccaccg gttgcttatg ccgtgccctg gactacgccc ggacctttaa ttgctttcct
     4861 cggaaccggg ggaaactggc tggcattact ggttggtttt ttatgtttag gtgtggcgac
     4921 aatgatctat ttaccttttg ttatcgccgc caacaaagtc aataacatgg caactaacgg
     4981 ataattccat ttacccagga aggagattgt gatgtttgca gatgaagaac ttgttatgga
     5041 gttactgatc aacgcgggac aggctcgttc tgatgcgatg gaagccatac gttgtgccgg
     5101 gcaaaaagac tggcagggcg caactaaact aatggccatc tctgaatctg cctgtcttca
     5161 ggcgcataag attcagaccg cgttaattag tcaggatgaa ggctgtggca aaatagaagt
     5221 taatttaatt ctgatacatg cacaggatca tttgatgaat gcaatattat gccaggattt
     5281 agccaaggaa attatttcat taagaaaaga gctccatgcc tgattttttc tccagtgcga
     5341 tatctggagg aaattactac actcttaagg ataagtcatg aatataaaga cgcttaacgt
     5401 cagccttttg tctatttcta ttattacagc attgtttccg ttgaacgcga tggcaacaaa
     5461 attaaccata gagcagcgcc ttgaattgct tgaaaatgaa ttgtcgcaaa ataaacaaga
     5521 gctgaaagca acacagaatg aactgggagt atataaattc cgactttcga cattacaaaa
     5581 aagcatcaca gaaaataaat atcaatcggc ctcgcttgcc gaaatatcag cgacatctcc
     5641 cgttgctgat aacatcaaaa atgaaaacgg tgaacagaac tcgtctgccg cagcacatnn
     5701 nnnnnnnnca catactataa atggatcgca gcaaattgcc gttattgaaa gtaaaggcga
     5761 taaaaccact atcgaaagcg tgaccctgaa agatatcagt aaatatataa aggatgatat
     5821 tggattcagc tatcaggggt acttccgctc aggctggggc accggtaatc acggctcacc
     5881 acaaacttat gcagcgggtt ctctgggacg ttttggtaac gagatgagtg gttggtttga
     5941 cctgacctta aatcagcgtg tttataatca ggacggtaga acggcaaatg cggtcgttac
     6001 ctatgatgga aacgtaggtc agcagtataa cgatgcctgg tttggtgaca gtgccaatga
     6061 aaatatcatg cagttcagtg atatttatct gacaacgcga ggttttttac ccttcgcacg
     6121 agaggcagaa ttctgggtcg gcaaacataa actcccgcaa tatgagatcc aaatgctgga
     6181 ctggaaaacc ttaaccacgg atgttgccgc gggtgtgggg atttaaaact gggcgcttgg
     6241 tgtagggctg tttgatatgt ccttaagccg agatgatgtc gatgtttact ctcgtgattt
     6301 tacgcgtacc agtcagatga ataccaattc tgtggatgtt cgttatcgca atatcccgtt
     6361 atgggatgat gcgacgttgt cattaatggc taaatattcc gcacctaata aaacggatca
     6421 acaacaagat aatgaaaatg acgacagtta ttttgaaatg aaagatagct ggatgctgac
     6481 ttctgtttta cggcaaaact tgcaacgcga tacgtttaat gaatttacgt tacaggttgc
     6541 caataattcc tatgccagca gttttgccag tttctcagat gccagtaata cgatggcgca
     6601 tggtcgctat tactatggtg accataccaa tgggatcgcc tggcgtttaa tctctcaggg
     6661 cgagatgtat ctgactgaca atattattat ggctaacgcg cttgtctatt ctcatggcga
     6721 agatgtttat agttatgaaa gtggcgctca tagtgatttt gacagtattc gcaccgtaat
     6781 aagaccggcc tggatctgga atacatggaa tcagacgggg cttgaattag gctggtttaa
     6841 gcaacagaac aaaactcagc agggnnnnnn nnnnacgtat atcaatattc tggataacga
     6901 attatcgaat tttaagttta atgagaacag caaagacgag tttatggccg gcatccaggc
     6961 ggaagtctgg tggtaatcta tttagttaag gagttgtgta tgtcaggatt taaagaagat
     7021 tttttatggg gcggtgcggt agccgcacat caattggaag gtggctggaa tgaaggagga
     7081 aaaggcatca gtatcgctga tgtaatgact gctggcgctc acggggtgcc gcgtgaagtg
     7141 acagaaggcg ttatcgacgg acttaattat cccaatcatg aagcaattga tttttatcat
     7201 cgctataaaa cagatattca gttatttgcc ggaatgggat tcaaatgctt tcgaacttcc
     7261 attgcctgga cacgaatctt tccgcaaggt gacgaacagg agccgaatga agagggttta
     7321 caattttatg atgatctctt cgatgaatgc ctgaagcagg gaatggaacc tgtggtgacg
     7381 ctttcgcatt ttgagatgcc ttatcatctg gtgacaaaat atggtggctg gcgaaaccgt
     7441 aaactgatcg acttttttat ccacttcgca tcaacggtct tcacgcgcta taaagcaaaa
     7501 gtaaagtact ggatgacgtt taacgaaatc aataatcagg tgaatttcag cgaaagcctg
     7561 tgtccattta ctaattccgg tatcttgtat tcgccagagg aagatctcaa tgagcgcgaa
     7621 caaataatgt accaggcggt annnnnnnnn ntatgagtta gttgccagtg ccctggcggt
     7681 acagactgga aaactgatca atcctgaatt taatatcggc tgtatgattg ctatgtgccc
     7741 catctatcct ctgacgtgtg cacccaacga tatgatgatg gccacgaaag cgatgcatcg
     7801 tcgttactgg tttactgatg ttcatgctcg tggatattat ccgcaacata tgctgaatta
     7861 ctttgcaagg aaaggattca acctcgatat cacaccagat gataacgcga ttcttgccag
     7921 aggttgtgtc gactttatcg gctttagcta ctacatgtct tttacgacgc aattttcgcc
     7981 agataatccg caactggatt atgttgaacc acgagatttg gtcagcaacc cttatatcga
     8041 tacatccgaa tggggatggc aaattgatcc ggcagggcta cgttattcac tcaactggtt
     8101 ctgggatcat ttccagttgc cgctgtttat tgtcgaaaat ggatttggtg cggttgacca
     8161 gagacaagct gacggcacgg tgaacgatca ctatcgcatt gattactttt cctcccatat
     8221 tcgggaaatg aaaaaagccg tagttgaaga tggtgttgac ttaattggct acaccacgtg
     8281 gggttgcatt gacctggttt ctgccggaac aggagaaatg aaaaaacgct acggaatgat
     8341 ttatgtcgat aaagacaacg aagggaaggg aacgctggaa cggatacgta aagcatcgtt
     8401 ttactggtat cgggatctca tcgccaacaa tggcgaaaat atatgagact tcaggaagat
     8461 attacggaaa tgtcaggctc tgtgatcaga gcctgacaat aactacatag cgtgttgatt
     8521 ataatagggc acgccgagcg catccgactt atcactgcca gtacccatgt gatttaaatc
     8581 aaacggtggt tgttcctgcg atgtgggaac aaccacgaca ttgccacagg aagtaacaac
     8641 cggctgcgtg gtttgttccg caacgccctg cgcagaaaag agaataagcg cagttgctat
     8701 tgcggataaa agtggtttca tgattgcctc agtgacgttg tcacgttatc aactagttgt
     8761 tgtaatgatt taatggatgt aaaaagcgcg attcaccctg catattggtc atccggtatt
     8821 tatgcggcgg aacatcaaag taatttttga aggttcgggt cagagtttgt tgggactcga
     8881 agccatatcg ttccgccaga tagagtatcg gctcgttact ttccttcagc ttttgcgcga
     8941 tttccgtcat cttacggcta cgggtgtatt ggcctaatga atgaccggtt tcttttttaa
     9001 acatccgttg caggtgccat ttggagtaac ccgaacgctc tgacactttc tccagtgaca
     9061 gtggcgattc caggttgtcc tcgatccagt ccaaaatgct atgaatggta atagcgtcag
     9121 tattgcgtct ggacatcgtc atacctcttt tttcgtttac ggcaggactt tcttaagcaa
     9181 atgctcaagt gttgccactt cgtccgccgt caggtttttt gttaattctt gatgcaggtc
     9241 ctggccaact aattgatggc attgttcaca tattgccgcg ccgctggtgg taagttttac
     9301 cagtacgccg cgcttatcat tcgggttcgg caacctttcc acccagcctt tacagaccag
     9361 gcgatccagc atacgggtca gtgcccccag gtcgaccgac aacacttttt tcagttcaac
     9421 cggagtaata cacgccgcgc agcggataga gcagagcacc ttaaactgtg ccgcggtaat
     9481 atccagcgga gacagatact cgttaagcag gcgatctttc ttctgattaa ccatatggat
     9541 taagcgaccc aatggaataa tttcattgaa cagatcgctg gtacttttca cattagttgc
     9601 cctggcaagt aattagttgc aggggataat attgcccagg caagtataag tcaaatgaat
     9661 gaattgaccg atgccacgtt ttgctaaatc ggttcaagtt ttggtcaatg cgatgcaagg
     9721 ttagctaaag agtgacactt tatccttgtt taccccttat acttttcgct gataaaccag
     9781 atacacagga taacaaccac caatgttaga tttgtttaaa gcaataggcc tggggctggt
     9841 ggtgttactg ccgttagcta acccattgac aactgtcgcg ttatttcttg gcctggcagg
     9901 caacatgagc agtgccgaac gtaatcgtca gtcgttgatg gcctcggtgt acgtatttgc
     9961 catcatgatg gtggcgtatt acgccgggca actggtgatg gatacatttg gcatttcgat
    10021 tcccggtctg cgaattgcag gcggcttaat cgtcgcgttt atcggttttc ggatgctctt
    10081 tccgcaacag aaagcgattg attcaccgga agcgaaaagc aagtcggaag agctggaaga
    10141 tgaacccagt gccaatattg cttttgtacc actggcaatg ccaagtaccg ccggtccggg
    10201 aaccattgcg atgattatta gctcggcctc cacagtgcgt cagagttcaa ccttcgc
//
LOCUS       DAWDRC010000058        10227 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3339_length_10227_cov_2.326878, whole genome shotgun sequence.
ACCESSION   DAWDRC010000058 DAWDRC010000000
VERSION     DAWDRC010000058.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3339_length_10227_cov_2.326878"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    611..620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2442 a         2569 c         2661 g         2545 t
ORIGIN      
        1 cgatcagctg gcaacgtttg gaacaaaatg gccgaaacgg gctgatttca ccccacacca
       61 ccgttttccc gcaggttggg caattcaccg taatagtttc tgacattttt actccttagc
      121 aacaggccag ttcgaaatcc agacgttctg gtacctgtcc gttttcactg tccagcggca
      181 taaaacgaat ggcaaaacgg ctcttatgac cggaaattgg cggataaagc tgtgattcga
      241 gcgacagatt aaggcgcagc aagtcggcat cgccaccgtt atcctgataa aaaccattca
      301 ggctggtttg tttacggaag ggggccgact ggcgaattaa atccagcacc atggtaagtg
      361 cctgggtaag tgggttcagg ctggcaatcc aggtttctac ctggctgtcg cgctgcgcct
      421 ggggtagatg cagccaaatg tgcaatgtag gtaaatcaaa gctgcaacag ccgcccggga
      481 tgctcagtcg ctgacgcacc agagcaatca aacgatcttc acgtagaaat tgcccgatac
      541 gcggcgcgga gattaatacg ctccccgccg cttttaactg ctgaattaat gcttcaatac
      601 ggctctggtc nnnnnnnnnn ttctttcaac agctcagtgc ggacttcgcc gcgctcgaaa
      661 acatccagta attcactgac attacggaag aaatgcagcg cgccagcgtg gtcaacgatg
      721 ggtagattaa cggtgagttg ctgaatcaaa aactcaatgc gcagccatgt acgcattttt
      781 tcatttagtg gatgttcaaa aaggacctgg gtctgcatta cggtttttcc tgtgagacaa
      841 actgcgacgc aagctgcaaa tagtatgcgt gcaggcgggc aacatccgat gcgatagcat
      901 ccggtgcgcc gttattatca atgacgtcat ctgccacggc aaggcgagct tcgcgcgttg
      961 cctgagcagc aaggatttgt tcgacatgct cgcgagttac atcgtcgcgc tgcatggtgc
     1021 gcttaagttg cgtttctggg ctgacatcca ccaccagcac tcgattcgct tttttataca
     1081 gtgagttttc taccagcaat ggcacaaccc atagtacata gggggaagta gcttgctgga
     1141 tctggtgttg cgtctcttgc tgaatcagcg gatgcagcag ggcgttaagc cagtttttct
     1201 cttccgggtt ggcgaagatc cgctcgcgca aggcccggcg ctgcaatgtt ccatcagcag
     1261 caatcatgtt agcgccaaag tgatcagcaa tggcatgtag cgcaggtgca cctggttcaa
     1321 ccacctgacg cgcaataata tcggcatcaa tgacgttaat tccgagatca gcaaacgcat
     1381 tggcaacggt actcttgcca ctgccaatgc ctcccgttaa ggcaactata tacctcataa
     1441 tttatattcc cgggaattca tcatgattat caaaacgtta aaaatgagcg cacgaaagcg
     1501 aaattgatga aacgttcgct cactatttac caggtaaatt tatgggattg tagcgtaaaa
     1561 aaagacaatt tcgcagtctt gcgccgcatt gattagtgcg tatgataacg tcactggagt
     1621 tgcgctctta cccttatagc cattaacccc aggaatccgc acatgcgtat tgaagaagat
     1681 ctgaagttag gttttaaaga cgttctcatc cgccctaaac gctccactct taaaagccgt
     1741 tccgatgttg aactggaacg tcaattcacc ttcaaacatt caggtcagag ctggtccggc
     1801 gtgccgatta ttgccgcaaa tatggacacc gtaggcacat tttctatggc ctctgcgctg
     1861 gcttcttttg atattctgac tgctgtgcat aaacactatt ctgtcgaaga gtggcaagcg
     1921 tttatcaaca attcttccgc tgatgtgctg aaacatgtga tggtttctac cggcacgtct
     1981 gatgcggatt tcgaaaaaac taaacagatt ctcgacctga acccggcatt aaacttcgtt
     2041 tgtattgacg tggcgaatgg ttattccgaa cacttcgtgc agttcgttgc gaaagcgcgt
     2101 gaagcgtggc cgaccaaaac catttgtgca ggcaacgtag tgactggtga aatgtgtgag
     2161 gagcttatcc tctccggtgc cgatatcgtt aaagttggca ttggcccagg ttctgtttgt
     2221 acaactcgcg tcaaaacagg cgtcggttat ccgcaacttt ctgcggtgat cgaatgcgcc
     2281 gatgttgcgc acggtctggg cggaatgatc gtcagcgatg gcggctgcac cacgccgggc
     2341 gatgtcgcga aagcctttgg cggcggtgcc gatttcgtaa tgcttggcgg catgctggcg
     2401 ggccacgaag agagcggcgg tcgcatcgtt gaggagaacg gcgagaaatt tatgttgttc
     2461 tacggcatga gctcagagtc tgcgatgaaa cgtcacgttg gtggcgttgc ggaatatcgc
     2521 gcagcagaag gtaaaaccgt taagctgccg ctgcgaggcc cggttgaaaa taccgcgcgt
     2581 gatattttgg gcggcctgcg ttcagcttgt acatacgttg gggcttcacg cctgaaagaa
     2641 ctgaccaaac gcaccacatt tatccgtgtg caggaacaag aaaaccgcat cttcaacaac
     2701 ctgtaatata ccaacgctgg cgtggagcaa cacgccagcg ttatcccatt ccactcatcg
     2761 catcgcctaa atggaaaatt ggcagataca ttgccaccac cagcgtaccg ataattcctc
     2821 ccgttatgat cagcaacgcc ggttccagta aggctgcgag gttatccgcc agcgccattg
     2881 tgttatctcg atgatgatgg gcgaggttgt ctaacatgag atccagcgag ccggatgcct
     2941 ctcctgttct cactaattgc aaacagagcg ggctaaactc accggcattt tttagcgcca
     3001 gccagatggg gtgaccgtta ctgatatcgt gctggatttg tgtcagaagt tgcacccagt
     3061 acgggcagcg cattgtttct ctgacgccct caacgccctg taaaaaagaa atgcctgcac
     3121 tttgtgtcag cgtcagaatc gtaaagatct gcgtgagttt ttgtccccgc atcagtgaac
     3181 ccataatcgg gatgcgtaac agcaatttct gccgtgcgat aagcctggtt gggtggcgca
     3241 tcagcagctt attggctatc gccagcagaa agccgaatag caccagcaac caaccccatt
     3301 cgccactaaa acttgccagc gtcatgatcc cctgcgttag cgccggtagc ggagtgttaa
     3361 aggtcttata gatagcggca aactccggca gaacaaaatg cagcattgcc acaaccacca
     3421 tgattgccat cgctaaaatg ataatgggat aacgtaacgc tgatttcact ttgtcggtca
     3481 actgacgctg ggctttttgc tgacgcgcca gttcaaagca gcattcatcc agcttaccgg
     3541 tcagttcacc cgtgcggatc atcgcctgat agagcggcgg aaatacctct gaccagggta
     3601 ataaggcatt ggaaaaggca acgccctgtt caagatcgtg cgccagtgat tgcagcaacg
     3661 cttgccactg cttactggga tgctgttccg ccagcagagc cagcccttca gaaagcgtta
     3721 acccggcttt gagtagcgtc gccagttgat gaatgacttc cgcgctttta tctcctcgcc
     3781 actgcgcaga attgatggcg attcgcttca ggcttagtgg ggtaaccatc tgttgctgta
     3841 gtgccatgag cagcaaagca cggctctctg cccatagcat cccatcttgc gcattgccgt
     3901 cgccggttat gccacgccag cgccagagtt gcttactcgc catgcggcat ccccaatacg
     3961 cggattaact cttcaaaggt ggttaagcct tgctccacgg ccaggcagcc gttttcaaaa
     4021 agcgttcgca tacccgcctg tcgggcgtgc gtttccagcg attcaacgtc ggtattagcg
     4081 gaaataagct gacgtatgac cggtgttatg ggcagaactt caaataaggc cgtgcgacca
     4141 taaaaaccgt ggtagcaatg tacacaaccg ggtgcctgcc agtggggcag cggcgacggc
     4201 catacattgt ctggaatatg gatgggctcc ccttgctgcc ggcgacaatg cgggcaaagt
     4261 ttgcgtacca gacgctgggc tattaccagc gtaagcgccg atgatagcat ccagcgggcg
     4321 actcccattt gctgtaaacg taccagcgtt tcgcagctgg aattagtgtg tagggtagac
     4381 aacaccaggt gaccggtttg cgcggctttt atggcaattt ctgccgtttc gccatcgcgg
     4441 atctctccga tcatgatgac gtcaggatcc tggcgcaata acgcacgcaa aacgccctga
     4501 aaggtgagtc cggcacgcga atggatttgc gtctggttta atccggctat ggggatctca
     4561 accggatctt cgacgctaca aatattaatg tcagcggtat tcagcatttg cagggcacta
     4621 taaagcgtga ccgttttgcc gctgcctgta gggccagtta ccagcaccag tccctgtggt
     4681 tgttgcaagg catgagcaaa gtccaccagt tgtgacggct gcattccaag cgtgttaaca
     4741 tccagtgcct ggttcacctg ctgtaacaac cttaatacca ccttttcacc accccgacat
     4801 gctaaggtcg caatacgaaa tgagacggcg tttcctgcca gttcgacagt gaattgcccg
     4861 tcctgcggca ggcgatgttc cgcaatatcc aggtttccca gcacttttaa tctggcggtt
     4921 aatgcgactc cggcatccgg tgaaacgtcc ggtaaaggat gcaatacgcc gtcgatacgc
     4981 aagcggatgc ggtaggcatt gtccgctggt tcgatatgaa tatcagacgc gcgttgttcc
     5041 agcgcagatt gtaacgttcg agtcagcaac tctgctttgg gctgatgctt ctcctgaaca
     5101 gctacgggca atgtctgttg tgtgcgactg gcgtgacctt ccatttgttg gcgcgtccag
     5161 caggtgatct caatacgttt ggtggtagcg aaatgcaatg cgtccagtag ctcatgcgaa
     5221 ggtgcatcga ctaccgcaac atgaaccacc tcttcgctgg catccagcaa gactccctga
     5281 taacgcagac acagggcagt gagctgtgga atattcattg ccgctcctta gttggcgtca
     5341 tcaaagcgga agacatcttc gcaggcttgc tgcaatgcgc tgtcactttg aatattgcag
     5401 ttacgcgtcc agccggtgac gccgtttgcg ttatcccaac ccggcgtcat gacgacgctt
     5461 agtccattga gactttcttg cccggtcagc gacaccacgc cctttgccac actcatggct
     5521 gaaacatagc gggtggtggt aggtgaggga atgccattgc tgccaccgtc gcaggtattt
     5581 aatccaccat gttccagcgc gcacaactct acggcggtac ggtaaggcac aaaggtttgt
     5641 agcatgtcgg tgagtgcggc tttgcgcagg tagttttgat aagcgggaat accaatggcg
     5701 cttaaaatgg caatgatgcc aataaccacc atcagttcga taagtgtaaa accgcgttgc
     5761 ttgtccatct gtttcgctcc ttgatttggt tggcgctact ttggcaaacg ccatcagcac
     5821 aagcgagggg caaaaaacga aacgggaaag cagcttccga ggttttttat ttcgttgcag
     5881 cgaaagacaa gaaatttgcg aggcgttacg aagaaagttg gggaagggga gatttatccg
     5941 cccgcgatgg agcggataaa tctgttagca attagcgaaa acgcattgaa aggtcgagtg
     6001 cctgtacgtg tttagttagc gcaccaacgg agataaagtc cacgcccgtt tcggcaaatt
     6061 cgcgcagtgt tttgtcagtg acgttgccag acacttccag tagcgccttg ccgttggtgc
     6121 gtttgacggc ttcgcgcatc tgttctgttt cgaagttatc cagcatgatg atatcggcac
     6181 ctgctttcag ggcttcatca agttcttcca gattctctac ttcgacttct actggcgcat
     6241 ccgggtgcaa ccaggacgct ttttcgaccg cctgccgcac tgagccggag gcaataatat
     6301 ggttttcttt gatcaggaag gcatcagaaa gccccagacg gtgattcgct ccgccgccgc
     6361 aaagtaccgc gtatttcaga gctgaacgca ggccgggtaa ggttttgcgc gtatccagca
     6421 actgagtgtc ggtgccttcc agcaattcaa catagtggcg taccttactg gcaactcctg
     6481 aaagggtttg cacaaaatta agcgcagtgc gttcgccggt taacagcacg cgggatgggc
     6541 cttcgagttc gaacaagggt tgattggcgt tgatgacatc gccgtcatcc acatgccaga
     6601 ttatggtgac atcgtcgcct gccagttgaa taaacacctc ttcaacccag cgtttgccgc
     6661 aaaatacgcc attctcgcgg gtgatcactg tggcatgaga gcgagagttt tccggtaaaa
     6721 gttttgccgt aatgtcattg ttggcatcga ctgttccgcc taaatcttcc cgcagcgcct
     6781 gggccaccgc gccggggata tcgagattaa tgcgttccag cagctcgtca cgtcgggtgt
     6841 cagggttata gcggcgaggc ggcatgttaa aactccagat agctaacgaa tcataaggta
     6901 gaaacatgct actctgaacc gggtattagc accacatata aggagatcct gcatgttgtt
     6961 agaacagggg tggctggctg gcgcgcgccg cgttccctca ccacattacg attgccgccc
     7021 ggatgacgaa acacccactc tgctggtggt gcacaatatt agcctgccgc caggcgagtt
     7081 tggcggtccg tggatcgacg cattattcac tggaactatt gatccgcagg cgcatccttt
     7141 ctttgctgag atcgcccatt tgcgcgtctc cgctcactgt ttgattcgcc gcgatggtga
     7201 aatagtccag tatgttcctt tcgataaacg tgcctggcat gcgggagtct ctcagtatca
     7261 ggggcgtgaa cgctgcaatg atttttctat tgggattgag ctggaaggca ccgatacgct
     7321 ggcgtatacc gatgcgcagt atcaacagct tgcggcggtt acgcgggcac tgattgatcg
     7381 ctatccggat atcgctaata acatgacggg ccattgtgat attgcgccgg atcggaaaac
     7441 cgatcccggc cctgcatttg attgggcacg gtttcgtgcg ctggtcagca aggagacaac
     7501 atgacgctat ttacaacctt actggtgtta attttcgagc gcctgtttaa gttgggcgag
     7561 cactggcagc ttgatcatcg tcttgaagcg ttctttcggc gggtgaaaca tttttctctc
     7621 gggcgcacat taggcatgac cattattgcg atgggcgtga cttttttact gttacgcgca
     7681 ttgcagggag tattgttcaa cgttcccacg ctgctggtgt ggctgctgat tggtttgctg
     7741 tgtattggcg caggtaaagt tcgtcttcat tatcatgctt atctgacagc tgcttcacgt
     7801 aatgatagcc atgcccgtgc cacgatggct ggcgaactca ccatgattca cggcgtcccg
     7861 gcaggctgcg atgaacgtga gtatttgcgt gagctgcaaa atgcattgct gtggattaac
     7921 tttcgttttt atcttgctcc gctgttctgg ctgattgtgg ggggaacctg gggacccgtt
     7981 acgctgatgg ggtatgcgtt tttgcgtgca tggcaatact ggctggcgcg ataccagacg
     8041 ccgcatcatc gtttacagtc cggcattgat ggcgtgcttc atgtactgga ttgggtgccg
     8101 gttcgtcttg cgggtgtggt atatgccttg atcggtcatg gtgagaaagc gttaccggcc
     8161 tggtttgctt cgctgggtga tttccatact tcgcaatatc aggtattaac gcgtctggca
     8221 cagttctctc tggcgcgtga accgcatgtc gataaggtgg agacgccaaa ggcagcggtt
     8281 tcaatggcga agaaaacctc gttcgtggtc gtggtggtaa ttgcgctact gacgatttac
     8341 ggggcgttgg tgtaaagatt attgccctca ccctgcacgg gtgagggcgt agagagatta
     8401 atgcgctttt acggctttgg cggttttctc tttaaacaga tagccgatac ctaacacgat
     8461 cagccatacc gggatcaggt ataccgaaat cgccattcct ggggtcatca gcataatcac
     8521 cagtaccgcc gccataaaca gcaggcagat ccagttaccc agcggataaa gcagggcagg
     8581 gaagcgagtt accacgcctt gttcttgttt ggcacgacgg aatttcatat gcgccagact
     8641 gatcatcgcc cagttgatga ccagtgcaga taccaccagc gccattaaca gtccgaaagc
     8701 ggactctggg gcaaggtagt taatcagtac gcacaatgcc gtaaccagcg cagacaccag
     8761 aatagtgtta accggtacgc cgcgtttatc gacagacgcc agcgcttttg gcgcgttccc
     8821 ttgttgtgcc agaccgaaca gcatacggct gttgcaatat acgcagctgt tgtacacgga
     8881 gagcgcagca gtcagtacca cgatattcag cgcattcgct acaaaggtat cgcctaactc
     8941 gtggaagatc agcacaaacg gactggtatc ggcggtaacg cgggtccacg gcattagtga
     9001 gagcagaacg gctaacgaac caatatagaa aatcaggatg cggtagataa cctggttagt
     9061 tgcttttggg atactttgct ccgggttatc agcttctgct gcggtgatcc ccaccagttc
     9121 cagaccaccg aaagagaaca tgataatcgc catcatcatc accagaccgg tgaagccgtg
     9181 tggcaggaaa ccaccctgat cccacaggtt gctaacgctc gcctgcgggc cgccgttgcc
     9241 gctgaatagc agccaggcgc cgaagatgat catcgctacc accgcgataa ctttgataat
     9301 ggcaaaccag aactccatct cgccaaacac ttttacgttt gtcaggttga tggcgttgat
     9361 caccacaaag aacacggcgg cagaaaccca ggttgggatt tccggatacc agaactgaat
     9421 gtatttaccc acggcagtca gctctgccat ggcaactaaa acgtacagta cccagtagtt
     9481 ccagccagag gcgaaaccgg caaaactgcc ccagtattta taagcaaagt ggctaaagga
     9541 gcctgcgaca ggttcttcga ccaccatttc acctagctga cgcatgatca gaaaggcgat
     9601 aaaaccagca atggcgtaac ccaggataat ccctggccct gcggactgta ttacggaggc
     9661 gctacccagg aatagccctg tccctatcgc gccacccagc gcgataagct gaatatggcg
     9721 gtttttaagg ccgcgcttta gctgctcgcc gtgctgttga ccttccatta tgaaacctcg
     9781 tgttgtgttg tttttttgaa ctacgcagca atgcgtgtgt aattttgtaa ttccgtttgt
     9841 tgtattggtt tctttacatt ttatgtaact gaattatgat gatagtactt tcaactatca
     9901 caagataatg gtatatgcgc gagaatgcac ctgctttatg tagatgtgtt gattttctgg
     9961 ataaaaaaaa agcaattgta agtttttcat cacatttaat agatacttta agtctgataa
    10021 catactgttt taactagcta tatgtcggat ttgtctgcct gctgaacacc atgccttagc
    10081 caccagcaca ataatcttac aaaaggtcat ataagaataa atagatgtta ataatacaat
    10141 ggggttgtaa cgcgagtatt taaatataat attgcgagct aagtgaaaac gttaagtaat
    10201 tgaaataatt tttcattaaa agcgttt
//
LOCUS       DAWDRC010000059        10226 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3340_length_10226_cov_2.300069, whole genome shotgun sequence.
ACCESSION   DAWDRC010000059 DAWDRC010000000
VERSION     DAWDRC010000059.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3340_length_10226_cov_2.300069"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2734..2743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7120..7129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9922..9931
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2456 a         2666 c         2600 g         2474 t
ORIGIN      
        1 acagataatg ctgctcgata tcgtgtttag cctggattcg gtgatcaccg ccgtcggcct
       61 ttctcagcat atatttatca tgattgccgc cgtaatgatt gccgtcggtg tcatgatgtt
      121 tgcggcgaaa acaattggtg atttcgttaa cgccacgccg tcaataaaaa tactggcgtt
      181 aaccttttta ttgtttgtcg gtgtattgct ggttgccgat agtctcaaca ttcatattgc
      241 caaagagtat ctctacttcg cgatattttt ctcattaagt gttgagacac tcaatattat
      301 ccgtgaaaga agaaagtttc gacattgatc gaaacgcatg aatcgcactc gcattaataa
      361 atctattacc aactttaaga tggagtctaa ctatgtctgt agcccttatt cgtgacggcg
      421 tcatccgtcc atccgaaaac gattcgcatg tgcttaccgc catgctgccc tcgtcctgcc
      481 cgcaaaacca cgcggcgaat attctgcctt tgccggatgg cgcgttaatg tgcgtctggt
      541 ttgcgggaac gcaggaaggt attgccgata tttctgtctg gggatcgcga ttgcccgctg
      601 gcggaatgca gtggagcgat gcggtcaaac tttcccacga tgacacgcgt tcagagcaaa
      661 acccggtgct gtttctcgcg ccggataacg tgctgtggct cctatggacc gcacaaatat
      721 ccggtaatca ggacaccgcc atcgtccgct accgtaagtc agacgacctc ggacaaacct
      781 ggggcgagat tgccaccctg cttgataaac ccggtacgtt cattcgacag ccaatcacgg
      841 tgcttgataa cggcaactgg ctgctgcccg tcttttattg ccgtacccaa cccggcgaaa
      901 aatgggtcgg caatgatgat atcagcgcag tgaaaatttc cgctgacggt ggtcactcct
      961 ggcgcgatgt cgaggtgccg caaagtctcg gctgtgttca tatgaatatc acaatgttgc
     1021 acgacggtac gttggttgct ctctttcgca gccgttgggc agacaacatc tatatcagcc
     1081 attcagtgga caacggcgag agttggtcgg tgccgcaagc aaccgaattg cccaacaaca
     1141 attcgtctat tcaggtaacg acgcttgcca gcggcgaact ggcgctggtg tacaacgcga
     1201 tgagtgcggc aggcgcagta gaacgtcgcg cgtcgcttta tgacgaaatc gacgatggcg
     1261 acgacagccg caaagaaccg acagccgtcg gacgctccgc tttctggggg gccccacgcg
     1321 cgccgatgac ggtagcgatt tctgccgatg gtgggaaaag ctggccgtgg cggcgcaatc
     1381 tcgatgaagg cgacggctac tgcatgacca acaattccct ggaaaagcta aatcgtgagt
     1441 tctcctaccc cagcatcaaa caaagcccgg acggtacgct acatatcgct tacacctggt
     1501 ggcgtcaggc catcaagtac gtacgcatct cccccgagtg ggttaagggg caggcatcat
     1561 gattatcggt aatctcaacc atttgtcgct ggcggggctt cctgcctggg tgagaaatat
     1621 tctgctgcgc ccggagtgtt cgttatcggc gttatccacc agagaggacg gtcgctggca
     1681 acctgaagga tgtcggtggt tctgcacgct gggaacttca gacacgcaac ccgccgaact
     1741 ccgccatacg gagtatcacc atttatgggc cgatatacag gtggtgatta ctggttgtga
     1801 gggcattaat gcgggaacgc ggcccatcgc tcgcgaaaac gatgaagaac gtaagcccga
     1861 tctgtttatc gccccatccc ctgaaaacag tgtcgccatc accctacacg ccggtgattt
     1921 cgccgtcttt atgcctgagg aacctcacca ggcgctgtgc gcaattggca cggctacttc
     1981 aatacgcaaa gcggtcttta aagtaccgcg cgacatgctg gaagcctaaa aggagagaaa
     2041 aaatgacaat ggaaatgatc ctggcattag gaatactggt cctgatgatc gtgctgatca
     2101 tgtcggacaa aatgcctttt ggcgcaccgc cactgcttgc gtgtttattg ctggttgtat
     2161 cgggactttc gaccgttcaa caggcttttg ccggattcgt caatccaagc gtggtgatga
     2221 ttgcagggtt tatggtggtc atggcggcgc tacaaaaaac ccggcttatc agcaacgtta
     2281 aatctgcgat gataagcctg gtcaataaag gcagttaccg cagctatggg ctgttgttag
     2341 tcattgttat gctgggtgcc agtcttgccg gcaccggagc gaccggttat tacgtgctaa
     2401 ttctgtcgct ggtatccacc attccgtaca gtaaaaagct accaacatcg aaattaatga
     2461 tgcccctggg atttgccacc aaccacccgc ttcttcctat caatctcgcg cttctctttg
     2521 gcgttacggc gaccgtactc gaaaccgcgg ggtttcatca ggagatttcg atggggcgct
     2581 ttgcgctggt gaatctcatc atgtcagcgg ctttcctcgc atggagtctt attgcctacc
     2641 gttttcttcc ggatcatccc attgcggatg cttccgagga tgcgctggcg gcacgcgagg
     2701 aaacgtttaa cgcccttcct gcctggaaag aatnnnnnnn nnnactattg ccgcattcgc
     2761 tgtcagcgtt atcggcatga tgctaatgaa cattctggga aatatcgcct tcgttattcc
     2821 gggtcttgcg ggtgcgtttg ttctgatgat cgatgtactg gacttcaaag aagttcgcga
     2881 tcatatgggg gctccggtca ttttaatgat ggcgggggtg atcggtgtag ctgatgcact
     2941 ggcgggcacc ggctttacgg cgatggttgg cgatgccgtt gcaggtgtgc tcggttctgg
     3001 cgtctcaccg tttgtattta ttgtcgcctt tgctttgctt accagtacct gcgccacctt
     3061 cacgggttcg aatatggggt cagtatatat ttttgccccc atcgccattg ccgcctgtac
     3121 tagccttggg cttaacccga ccgctgccgc gattgcggtg gtgatttctg gctggaatgg
     3181 cggctatatg cctattgacg gaatgccagc catgatcctc ggaatgggga aatacaaact
     3241 tcccgaattc tgggtgttct ctgtaccaat gtatctcatc cacattctgg cgctttgtgc
     3301 aggcgctgtt tttatcttcc caatgtaatc tcagacaaaa aaagcggcag agaagatccc
     3361 tgccgctttt tattgttgga tgcgacgctg ccgcgtcgca tcagacataa agcagattac
     3421 tttttgattt catacagcgg tgtttgaccc gccacaacat ggccctgagc tttaatgata
     3481 aggccgctga aatcgtcgat attgctgcaa accaccgggc taatcatcga gcgagcgtta
     3541 gcgttcaggt aatccagatc catttccaga atcggttgcc ctgcgcttac ctgcgcgccc
     3601 tcttccacca gacgtttaaa gcctttacct tccagcgcta cggtgtcgat acccatatgg
     3661 acgacgatct ccgcgccttt ttcggtttcc agacagaatg cgtggttggt gttgaagatt
     3721 ttcacgattg ttcctgcggc tggtgatacg acgattttat ctgtcggttt caccgccaca
     3781 ccgtcaccca ccgctttgct ggcgaatgct tcgtcaggaa cctgatccag tgccacaaca
     3841 tccccggtaa tcggcgatac cagctccgcg atagataccg cgtttggtac agcctgcggt
     3901 tttgctacag gagcggcagt tgccggagtt gcttcagctg acgcagcggc taccggacca
     3961 cgggcaacga ctttcttcat cgcatcgccg atggattctg ctttcgcgcc aacaatcacc
     4021 tgaatagtct gtttgttcag tttcactacc ccagaagcac ccagacgttt acacatcgca
     4081 tcgttgacgc gagctgagtc aaccacggta aggcgcagac gagtaataca ggcgtcaatg
     4141 gctttcaggt tgtcagtgcc gccaaccgca gcaatatagt tggttgccag ttgattgaga
     4201 ccttcttcag tattgctgtt agcctcttca gtaacgatct cgtcttcttt atcttcacga
     4261 cccggcgttt tcaggttgaa catgcggata accaaactga acaccacgaa gtagatagcg
     4321 aagaagacaa cccccatcac cagcagcatc cagacgttct ggctggcggc cggcaggtta
     4381 tacatcaacg cgtagtcgat agcccccgca gagaaagaga agccggcatg gatacccagc
     4441 agcgttgcca caaacaggct gataccggtc agcagtgcgt gcaggaggta cagcagcggt
     4501 gcaaggaaca tgaacaggaa ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca
     4561 gcaacagaaa gcagcatccc gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac
     4621 atcgccagcg ccgcacccgg cagaccgaac atcataatcg ggaagaagcc ggacatgaac
     4681 atccccgcgg tgccgtcacc ggcatagaag cggttgatat caccgtggaa aaccgttccc
     4741 gccgcgttgg tgaattcacc aatctggaac caggcgatgg tgttcagcac ctgatgcaga
     4801 ccggttggga tcagcagacg gttgatgaaa ccaaagatac cggaacccag cgcgcccgca
     4861 gaaacgatcc actcgccgcc tgcatggata gcgtgctgta ccggtggcca gacgtaacca
     4921 aaaatggccg ccagcaccag acagaagaag ccggtggcga tcggcacaaa gcgtttgccg
     4981 ccgaagaagc tcaggaagtc cggcagttta atatcggacc aacggttata ggctgcgcca
     5041 ccaaccagac cggtaatgat acccgccagt acacccatgt taatttctgg gttgatggtc
     5101 accatcgctt tggttaacac aaagtaacct accgcacccg ccagtgccgc cgcacctgcg
     5161 ctgtctttcg accagctgga tgccacgccg atggcgaaga ttaatgcgag gttatcaaaa
     5221 atcgcaccgc ccgcctgggc aataaacgca acgttaagta aatctggctg accgaatcgc
     5281 agcaacagcg ccgccaccgg cagcaccgcg atagggagct gtaacgccct accgagtcgc
     5341 tggaaaaaac ctaaaatatt catcttattc cccctacgag aaccctattt ggctcgttta
     5401 aagccgtatt tttattttgc tgcaaattgt actgccgatg ttctgtaatc agattgttag
     5461 atcatctgct acagagtgtg tgaaaattta attcgtatcg caaattaaac gcgtgtcttt
     5521 tgtgagtttt gtcaccaaat atcgttatca tcactccctt ttactggcta aaccagaaaa
     5581 cttattttat cattcaaaaa atcaggtcgg attgacgcct gtctgcgcaa atccaggtta
     5641 cgcttaaaga tgcctaatcc gccaacggct tacattttac ttattgaggt gaataatgag
     5701 actgatcccc ctgactaccg ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa
     5761 tcgtatcaat gcgttcaaac cgactgccga tcgtccgttt gtactgggcc tgccgactgg
     5821 cggcacgccg atgaccacct ataaagcgtt agtcgaaatg cataaagcag gccaggtcag
     5881 ctttaagcac gttgtcacct tcaacatgga cgaatatgtc ggtctgccga aagagcatcc
     5941 ggaaagctac tacagcttta tgcaccgtaa tttcttcgat cacgttgata ttccagcaga
     6001 aaatatcaac cttctcaacg gcaacgcccc ggatatcgac gccgaatgcc gccagtatga
     6061 agaaaaaatc cgttcttacg gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg
     6121 tcatattgca tttaacgaac cggcgtcttc tctggcttct cgtactcgta tcaaaaccct
     6181 gactcatgac actcgcgtcg cgaactctcg tttctttgat aacgatgtta atcaggtgcc
     6241 aaaatatgcc ctgactgtcg gtgttggtac attgctggat gccgaagaag tgatgattct
     6301 ggtgctgggt agccagaaag cactggcact gcaggccgcc gttgaaggtt gcgtgaacca
     6361 catgtggacc atcagctgtc tgcaactgca tccaaaagcg atcatggtgt gcgatgaacc
     6421 ttccaccatg gagctgaaag ttaagacttt aagatatttc aatgaattag aagcagaaaa
     6481 tatcaaaggt ctgtaattat tatccctgcc ctgacacctt gctcagggca atatttttta
     6541 aaatcggggg tcagaatgta tgcattaacc cagggccgga tctttaccgg ccacgaattt
     6601 cttgatgacc acgcggttgt tatcgctgat ggcctgatta aaagcgtctg tccggtagcg
     6661 gaactgccgc cagagatcga acaacgttca ctgaacgggg ccattctctc ccccggtttt
     6721 atcgatgtgc agttaaacgg ctgcggcggc gtacagttta acgacaccgc tgaagcggtc
     6781 agcgtggaaa cgctggaaat catgcagaaa gccaatgaga aatcaggctg tactaactat
     6841 ctgccgacgc ttatcaccac cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc
     6901 gagtacctgg caaaacatca gaaccaggcg ttaggtctgc atctggaagg tccgtggctg
     6961 aatctggtaa aaaaaggcac ccataatccg aattttgtgc gtaagcctga tgccgcgctg
     7021 gtcgatttcc tgtgtgagaa cgccgacgtg attaccaaag tgactctggc accggaaatg
     7081 gttccagcgg aagtcatcag caaactggca aatgccgggn nnnnnnnnnt tgccacccat
     7141 ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc
     7201 gacgaagctg acatttattg cggtattatt gctgatggcc tgcatgttga ttacgccaac
     7261 attcgtaacg ctaagcgtct gaaaggcgac aaattgtgtc tggttaccga tgccaccgcg
     7321 ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata ctaccgtaat
     7381 ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat gattgaaggc
     7441 gtgcgtaatc tggtcgaaca ttgtggtatc gcactggatg aagtgctgcg tatggcgacg
     7501 ctctatccgg cacgtgcgat tggcgttgag aaacgtctcg gcacgctcgc cgcaggtaaa
     7561 gtagccaacc tgactgcatt cacacctgat tttaaaatca ccaggaccat cgttaacggt
     7621 aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct cagataggta
     7681 atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt gaccagtacg
     7741 ggccaatctc gcggattcag attgccgagc aaagtcagct tgcccccgcc agcgtaacca
     7801 aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag caggcctcca
     7861 ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac gcaatcggcg
     7921 tacggcttgg tcgtcacgac gccaccatca ccttgtttga tctcagcagc aaagtgctgg
     7981 cagaagaaca ttactcgctg ccggaacgta cccagcagac gctggaacat gccctgctga
     8041 atgccattgc tcagtttatt gatagctacc agcgcaagct acgcgagctg atcgcgattt
     8101 cggtgatcct gccagggctt gtagacccgg acagcggcaa aattcattac atgccgcata
     8161 ttcaggtaga aaactggggg ctggttgaag cactggaaga gcgttttaaa gtgacctgtt
     8221 ttgtcggcca cgatatccgc agtctggcgc tggcagagca ctacttcggt gcaagtcagg
     8281 attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg attatctcta
     8341 acgggcgaat ttttatcggg cgcaacggca acgtcggtga aattggccat attcaggtcg
     8401 aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact atcgccgcca
     8461 atgctgccat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag agccgtgtgc
     8521 cactggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc gatagcctgg
     8581 cctcggaagt gattgaatat gtcggtcgtc atctgggtaa aaccatcgcc attgctatca
     8641 acttatttaa tccgcaaaaa attgttattg ctggagaaat caccgaagcc gataaagtgc
     8701 tgctccctgc tattgaaagc tgcattaata cccaggcgct gaaggcgttt cgtactaatc
     8761 taccggtggt acgttctgag ttggatcacc gctcagcaat cggcgctttt gcgctggtaa
     8821 aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa tgtgctttta
     8881 tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa atgtaatttg
     8941 cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag cggaattttt
     9001 gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc cttcgcagac
     9061 cgggcaagat ctggcgaacc gctttgccac cgctggtgtc gatgtaccgg atagcgtgtt
     9121 ttatacctcc gcgatggcga ccgccgattt cctgcgtcgc caggaaggca agaaagcgta
     9181 tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca ctattaccga
     9241 tgtgaaccct gattttgtga ttgttggtga aacgcgttcc tacaactggg acatgatgca
     9301 taaagcagcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc cggacaccca
     9361 cgggcgcggt ttttatcccg cctgtggcgc gttgtgtgca gggatcgaga aaatctccgg
     9421 acgtaaaccg ttctatgttg gtaagccgag tccgtggata atccgcgcag cattaaacaa
     9481 aatgcaggcg cattcggaag aaacggtgat tgtcggcgat aacctgcgta ccgatattct
     9541 ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt catcgctcga
     9601 cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcggtcg ctgaaatcga
     9661 cgttatctga atagcaacca cgtctgcgga cgtggtttct tatttttacc catcattata
     9721 atcatcacat tcaataaaac ccgccaaaaa ttatcgatcc atcaataaac acgtcaccac
     9781 accctccctt ttttcacaaa tcagcaatca ctattacggt ttttattttt ccactgccaa
     9841 atagcttgcg cacggtgctg ctttgcggca tttttttaaa caagcaaaca caacaagcaa
     9901 caaataccag gttaacggag annnnnnnnn ntcacgccgg atgcgtcatc gtggcccgga
     9961 ctggtccggt atttatgcca gcgataacgc catcctcgcc cacgaacgtc tgtcaattgt
    10021 tgacgttaac gcgggggcgc aacctctcta caaccaacaa aaaactcatg tgctggcagt
    10081 aaacggtgaa atctacaacc accaggcatt gcgcgctgaa tatggcgatc gttaccagtt
    10141 ccagaccggg tctgactgtg aagtgatcct cgcgctgtat caggaaaaag ggccggaatt
    10201 tctcgacgac ttgcagggca tgtttg
//
LOCUS       DAWDRC010000060        10126 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3383_length_10126_cov_2.359249, whole genome shotgun sequence.
ACCESSION   DAWDRC010000060 DAWDRC010000000
VERSION     DAWDRC010000060.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10126)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3383_length_10126_cov_2.359249"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5159..5168
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6995..7004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8435..8444
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2506 a         2421 c         2544 g         2625 t
ORIGIN      
        1 catccgaacc cgtgtgaccg gcttcactcc tggccgaatc attacctgga gcagtatccc
       61 cgaaaggttg cctgttacct cgccagcatc agtaaactgg ataactttct cggtcagctt
      121 gatggtatcc ttcgccggca ttcccgtcat ttcgtcatgc tttacttttc tgaccatggg
      181 ctgtcggtca gcgacagtgc taatcctgtt catcattatg gtcatgtgca gggaggctac
      241 agcgttcccc tgattattac cgccagtgac atcacgtctc atcagtccgt cagcagaaaa
      301 atcagtgccc gtcatttcgc aggtattttt cagtggctga ccggcattcg tactgaaaat
      361 ataccgccat tcaatctact gacagacgaa gataatgaac ccattatggt ttttaacgga
      421 gagaggaatg taccggcaga cagtctgaaa ccgcagcctc tgattcttcc ggtcaaggga
      481 aaataagcgt aatgttcatc accagattat gtacgatgag ttttccactg tttatagcaa
      541 ttatcttgct aataaccaga caggagtaac cagaccaacc gaaaacaccg gggaggtgat
      601 tacccgctgg cagaggaaat ccggtttttt ttgatgtcag ccataacgtg tttgacaata
      661 aaacattatg aaagggtgtt aatgagtcca tgggataatt cataaaaata atttattgtg
      721 ttatcctttt caaaagaaat attcagaaga taacccctgg ctgacaacca ggggaagggg
      781 acattaacag tgttattatt ggtggataaa ttattacttg tgtgcaggat acactaatgc
      841 aatatctact gatactgtat gccagtatgt ttcatgcctg ccgctgaaaa cggcaatatc
      901 cagggccaga catattcacc attcatattt ctgcatttct gtacaatttt ctcagaaagc
      961 gtctcatcaa ttctttcacg caacttctcc tgctgtctta aaatactttc gcatatttcg
     1021 aaatcatctt cctgcatcag catttcggcg catttaatga tttctgcccg ttcatgcagc
     1081 acagaggaat gattatagag ccagtttaca aacagcaatg cttccggata ttgcccccat
     1141 aacgggtggt atgtggcata ataatcttcg gcagtatccg gaccgcctga aaagttatgc
     1201 catacagcaa gtggaaatgg atgcagattt ccgcacagta tttctgcgat gtaagtattt
     1261 gccgcaaagc cgcgacgaaa tgcagttgca gcttttacca gttccccatt aagaaaatgc
     1321 agaaggccga gttcatacca gtaaggtgaa aattcatctg catgttcctg cagtatatgg
     1381 cgtgcctgtt catgagcgcc agtgcgtaac agttccggtc ccagtagcca gcgtgcacca
     1441 tgattatctt cgggattgta atccagtatt ttttcaatga gcatgatggc atcctgatga
     1501 cgccgtaaat gtgcatttgc caggattgcc gcatagaggg cacgaagaaa aggtcggttg
     1561 tcaggatgta tccagatgat tctgccactg aaaccttcag ggataagacg attgccaact
     1621 gccagtcctt taagggcagc attcagcgcc ttacgcgggg cattctgctc cagaaatacg
     1681 tatgccaagt gagcgtgtac atcaataaag tcattctcct gtcgggcaag gcgcctgagt
     1741 ttgttgatgt aacttgtttc gctgatttca tccgcatcgt atgcatcaat cagttcttca
     1801 aactcatcca gcaacgagcc aaaccaggtt tccggaaata tgaaacagcc ctggttatcg
     1861 ttcacttcaa agcgtaattt gccagtcata ttctgaacct gtaaaaaagg atagaccata
     1921 atctgcaggc tataaaaatt gtgatgcctg gcatcggtgt ccttttattg tccgggatta
     1981 acgttgccca tgataataca gtgaatcctg ttctgtggta agacgttgag ttattgaatg
     2041 agaacctcag cctggctgat ggttccgccg gcataaaaaa gaaattgatt gaggcgggtg
     2101 ctcttcccct taagaggaag agcatgtgtg taaatggtgt tcagtcacct tttcagtgga
     2161 ttgttaactt ttgcatattc aaacggactg atacactcct ccctgtttgc atccagaaaa
     2221 tctgcccacc attgcagcat aagccgacgt tcttccagat gttctgcttt atgaatatac
     2281 gcggcacgta ctccgttgcg ctcctgatga ctcatctgac gttcaaccgc atctcttgac
     2341 cataaaccgg attcaatcaa tgcactgcac gccatcgccc ggaatccatg gccacagact
     2401 tccttggttg tgtcataccc catcactctg agggccttat ttacggtatt ttcactgatt
     2461 ggtttacgcg ggtcatgtgc tcccgtaaag atcagaccat tttcaccagc ccatgtctga
     2521 agttctgtca gtatggctac agcctgtttg ctgagtggca caagatgtgg tgttcgcatc
     2581 ttggagcccc ggtgtgagaa ttttacccct ggcagaggtt tgcgttcggc tggaatagtc
     2641 cacatggcat tgtcaatatc aatttcagac cagcgggcgt atctgagttc actggaacga
     2701 ataaaaacca gtaaattcag cataacggca agccgggtga caggctgacc tttataactg
     2761 ttaatacgcg acagaagttc aggcagacgt gaaagatcaa gagcggggcg atgctggcgt
     2821 tttaccgtag tcaatgcgcc agccatatcc agagcaggat tatatctgat gatgccagac
     2881 tgtacggcat aacgcattac ggcagatatt ctttgctgca gacgactggc gatttcataa
     2941 atttgtttag cttctgcagc acgaacaggg ataagcagat ccggggtatt gagtgttgtg
     3001 atatcccggt tgccgataaa ggggaaaacg tgtgtttcga ggcttttgag tactttatcg
     3061 ctgtgtgatt gtgcccattg tttattactg gcatgccagc gtcgtgcaat atgttcaaaa
     3121 gagagctgta aatcagggac tactttttta gctcttttct gttcgcaagg gtcaatcccc
     3181 tccgaaatca gttttcgggc ctcatcccgt tttgtacgag cttcggagag agaaacttct
     3241 ggatacacac caagtgccag agtcttctcc ttacccagaa tacgataacg gagacgccag
     3301 taacgggaac cattaggatg taccagcagg aacatgccag ccccgtcagt gagtttataa
     3361 gccttgtcag tgggctttgc agcccggatt tttgcgtcag tcagtgccat agttcagcct
     3421 tctgaatgcg ttaaagaggg tacaaattct gtcgaactgg ggtatacccg caattatacc
     3481 cacacaatgg ggttgatgtg ggggtaatct ggttgagttc ggtagaattg atttggagga
     3541 agaacactta aatcatggtg ttctgaaacg aaaaacggac ctccgtggag gtccgttgat
     3601 atgaatttgg tgcccggact cggaatcgaa ccaaggacac ggggattttc aatcccctgc
     3661 tctaccgact gagctatccg ggcaacgggg cgcattaaac cgtaatatca gcgcggcagc
     3721 ccacgccagt gttaactgga tgatcagtaa tggaatgcta atcaccgaca ccgcaatacc
     3781 cagcgagcag gcgaagatca tcgccgtaaa gaaatcaaga aatgacttgg cgattaaaat
     3841 actcggatcg ccggtcatcc cttcgttcat cgccccgaag attccggtgc cgctggcgca
     3901 aaacaggaca ataatcgcga caaaattctg aataaaagat tcatgcgctg gcttcttagg
     3961 cgagtggtga aacagattat gcgctttggc gaccactgta ttgacgcctt tttccagcag
     4021 acaaatttcg ccgattaatg ctccgagtaa ggttgctaaa accatcgccg gaaggttggc
     4081 gcatttcacc accagtaaaa taccaatccc cagcgatgcc agaccaaaaa ttgatgtcat
     4141 ggagacgcgg atacgttccg gtaagcgttg gctaagaagt gcgccgaaaa cgccacccag
     4201 taagactgca cttgcgttga taaaagggcc gatgaccaca agcgttcctg ttagctgtat
     4261 tgtttgattt tcttattatg aacttttggc gccgtcgatg gcttgtttgt tcgcaaagtc
     4321 ctggcttgca cgctttagcg aaaggtgcca tgattgcgcg aattttctcc tctctgtacg
     4381 gagtttgccc gatgcacgcc acctccttac attctctcgc ttatcgccgt ttcgcgcgaa
     4441 actcttctct ttttatgcta ctgctcatgc ggtgaagtta acgcacgctc actgcaggac
     4501 aacagtaaaa tcagagcgtt tctgctttta ctgatgtctg gcggtcggag ctggtgacca
     4561 gtttgaccca tatctcgtgg ggcagggttt ttcaccttgt ccggtattct tacttccccg
     4621 aaacgggttt tgcgcttatg aaatcaatga atattgccgc cagtagtgaa ctggtatccc
     4681 gactttctac tcatcgctgc gtggtagcgt tgggagatac tgattttacg gacgtcgcgg
     4741 cagtcgtcat taccgctgcg gatagtcgca gtggcattct tgcgttgctt aagcgcaccg
     4801 gttttcatct accggtgttt ttgtattccg aacatgctgt tgaattacct gcgggcgtta
     4861 cggcggtaat caacggcaac gagcagcagt ggctggagct ggaatccgca gcctgtcagt
     4921 atgaagagaa tttgctgcca ccgttttatg acacgctgac gcagtacgtt gagatgggta
     4981 atagcacctt tgcttgccct ggacatcaac atggtgcgtt ctttaaaaag catcctgccg
     5041 gacgccattt ttacgatttc tttggcgaga acatctttcg cgccgatatg tgtaacgctg
     5101 acgtaaaatt gggcgatctg cttattcatg aaggatcggc gaaagatgcg cagaaattnn
     5161 nnnnnnnnaa atttgcagcc aaagtctttc atgccgataa aacctatttt gtgctgaacg
     5221 gcacatcggc agcgaataaa gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc
     5281 tcttcgaccg taacaaccat aagtcgaatc atcacggcgc gttgattcag gcgggggcta
     5341 cgccggtcta tctggaagct tcacgtaacc cgtttggttt cattggcggt attgatgcgc
     5401 actgttttaa tgaagagtat ctgcgccagc aaattcgcga cgttgtgcca gaaaaagccg
     5461 acctgccgcg cccgtttcgc ctggcgatta ttcagctggg aacctatgac ggcactgtct
     5521 ataacgcccg tcaggtgatc gataccgttg ggcatctgtg tgattacatt ctgtttgatt
     5581 ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc ggatagctcg ccgctgctgt
     5641 tagaacttaa cgaaaacgat ccggggatct ttgtgaccca gtcggtgcac aaacagcagg
     5701 cgggattctc acagacgtcg cagatccata aaaaagataa ccatatccgt ggacaggcgc
     5761 gtttttgccc gcataagcgg ttgaataatg cctttatgct ccatgcttct accagcccat
     5821 tctatccgct gttcgccgcc ctggacgtta acgccaaaat tcatgaaggg gagagtgggc
     5881 gtcggctgtg ggctgagtgt gttgagatag ggattgaagc gcgcaaggct attcttgcgc
     5941 gctgtaagct gtttcgcccg tttatcccgc ctgttgttga tggcaaattg tggcaggatt
     6001 atccgacatc agtgttagcc agcgaccgcc gttttttcag ttttgagccg ggggcgaagt
     6061 ggcacggctt tgaaggatat gccgcggatc agtattttgt tgatccgtgc aagctgttac
     6121 tcactacacc aggtatcgat gctgaaaccg gcgaatatag cgactttggc gttccggcga
     6181 cgattctggc gcactatctg cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact
     6241 ccattctgtt cttattaact ccggcggaaa gccacgagaa gctggcacaa ctggtggcga
     6301 tgctggcgca gtttgaacag catattgagg atgactcgcc gctggctgag gtgttgccga
     6361 gcgtttataa caaatatccg gtgcgctatc gcgactacac cctgcgtcag ttgtgtcagg
     6421 agatgcacga tctgtatgtc agtttcgacg tcaaagacct acaaaaagcg atgttccgcc
     6481 agcagagttt cccgtcagtg gtgatgaatc cccaggatgc gcatagcgct tatattcgcg
     6541 gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg aattgcggca gaaggggcgt
     6601 tgccttatcc acctggcgtg ctttgcgtgg tacccgggga agtctggggt ggggcggttc
     6661 aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt gccgggattt tcgccggagt
     6721 tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa acggttgtac ggttatgtgt
     6781 tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc gtttttcttt gcgtaattag
     6841 tggctaaccg tctgtgtacc tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg
     6901 aacgcgaagg ccagaaccac ggagtaacct gcgaaaatca gccatacggt ctgccagtcg
     6961 gtaatgccgt tttgggtgta catctcaacc acttnnnnnn nnnngctcac gatgccgccg
     7021 aggatacagc cgaagccgtt ggtcatcatc aggaacatcc cctgcgcgct ggcgcgaatt
     7081 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
     7141 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
     7201 taagcaaaca gagcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
     7261 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
     7321 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
     7381 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
     7441 agcagcattg agaagatgaa gaagattgcc atgcgcttgt ttttaaacag cgcgaatgca
     7501 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     7561 ggcagagtca gggtaaacag aaccagaatg gcggaaagcg ctgcgccaat atacagctgc
     7621 atgtggctta attcgaagcc ggacaggctc accacccaca ttgccatgat aaagccgatg
     7681 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     7741 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     7801 aatatcacaa ggaacatcgc ctccggcgta gtgacctctg ccgccatgaa cagcgtgata
     7861 gcgccaatgg tgtggcaaat ggcatagacc catttcgcac ttaaccattt gtcggccaca
     7921 atccccagca gcgcaggcat aaagaccgcg gcgataccca gtgagctata aactgcgccg
     7981 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     8041 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     8101 ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     8161 atgatgcgat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     8221 aacattccca atttgcaaaa cgaaattgtg atgtagataa catttttacc cctgagcatc
     8281 gtcaggggcg gttagtggaa ttatcggcgg cggtaagatt tttgcgcggt attcacttta
     8341 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     8401 cccggcgtca tggtgttaat aatattggca gcctnnnnnn nnnnggcagc ctgaatctta
     8461 tcattcgaaa agactcgtag cacgctgcct gcgccgccgt tataggcggt gatgacggca
     8521 taacgccgcg atgttgggtt atcaattccg ccgagataaa cgttgttcag catcgccaga
     8581 tacgcggtgc cggtatcaat attgctggca ggatcaaaca agaaactgcg gctcggcgtg
     8641 ccggatttcc cctgcgagcg gaacacatct ttcccggcgg tatgttgtac cacctgcatt
     8701 aatcccagcg catcggaacg gctgaccgca tacgggttaa aggaagattc agtctgcata
     8761 attgccagaa tcagcgactc atcaacgcca tatttgcgtg acgcctggcg gaccataccg
     8821 agatatttgt gcgcacgttt atcaaggtgg ttcggcacca tgttaatggt gacgctgtag
     8881 atgatacgca gcccgttgct gcggctcttc agacggtttt tcagcagata atccgcgaag
     8941 ttgctcgcgc gaccttccca gcgaatcggc tgcccggtgt tgtccaccac ctgaccgtaa
     9001 aggaaaggtt ctttcgaaat cgtaatatca tcaacgtcgg aatagagatc gaccgaactc
     9061 ggatcgtcac ccatcagcaa cgttttgatt attgcccggc gcaaatgcgc ggcaggttct
     9121 gtcccggcga tggtttcgat agtaatcgta ccgtcatcga agttgatgtg gctgcgggtc
     9181 tgatattgat cggtgtattt cacgtagtcc ttaggaccgg cgatcaccac ctctttgaag
     9241 ccccagatgt tctcaatatt gtgggcaaat tgccccatca gaatatcaaa accgttggta
     9301 tctttgaccc aggcttcgtt ataggtatcg ccttttttgg tcgtcgaaca ggagatgagc
     9361 aacggcgcaa tcaaagccag cgcgagatat tttttcatca ttccgggtgc gtgttgtgtt
     9421 tgtttttatg caagttgccg gaggcgtgct ccggcactgt ttttattttt tatcttccgg
     9481 cgtatagccc tcgatatgca cctctttacc ctcgaacagg aagttgacca tctcctgctc
     9541 cagcagcttg cggtgctcgg cattcatcat gttgagtttc ttttcattaa tcagcatggt
     9601 ttgtttgtgc tgccactgcg cccaggcttc tttggagatc tcgttataga tgcgttttcc
     9661 cagttcgccg gggtacagct gaaaatcctg accttctgct tcacgttgca ggaaagtaca
     9721 aaaaatcgtt ctgctcataa atcatcctct ttatcgactc acgcgctaaa ccggcgcgcc
     9781 agtgcgtaac tgctgtaaca aacgctccac gggagccgcc aggccaactg acggcggttg
     9841 cgctaagtta taccagagcg cattgtcttc atccatgcag ccggtgaatg acgacacggg
     9901 aagccacata ggcacaatat ctaagtggaa atggctgaag gtatgccgaa acgcggtcag
     9961 ctgcgtcagg ttatcggcag caatctgtcg ttgcgccagc cactgccgca aactttcttc
    10021 atcggcaaac tgcgggaaac agtataaacc gccccacaat ccactcggcg gacgctgcgc
    10081 cagcaatact tcatcttcgt gctgtaacag cagaaagtag ccggtg
//
LOCUS       DAWDRC010000061        10050 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3406_length_10050_cov_2.198699, whole genome shotgun sequence.
ACCESSION   DAWDRC010000061 DAWDRC010000000
VERSION     DAWDRC010000061.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 10050)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3406_length_10050_cov_2.198699"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3883..3892
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2282 a         2550 c         2788 g         2420 t
ORIGIN      
        1 gccgagttgg cgcgcgtatt ccagcccggc aatcacatac ggcgtgcgtc cgctggccgc
       61 aataccaacc accacatcct gcgcggttaa gccgatattt ttcagatcat taacaccacc
      121 ttcccggcta tcttctgcgc cttccaccgc gtgctgaatg gcatattcgc cgccagcaat
      181 caaaccaact accaggcccg gtttcacgcc gtaggtcggc ggacattcgc tggcatccag
      241 aatccccaga cgaccggatg ttcccgcacc gagataaatc agacggccgc cgccgcttac
      301 ctgggtgtgg ataacatcga tcgccgcggc gatatccggc agtacgcgct caacggcgag
      361 tggtacggtt ttatcttcat cgttgataat ccggcacatt tccagcgttg atacgcggtc
      421 aatttcagcc gaggcggcgt tcgagccttc ggtaatcatc ttttcaggtt gcatcggggt
      481 gaactttaaa tttgcgatca aataattgca gataaatggg atcatatatc ccgatttgat
      541 agacacaaag gaatcattaa gtccgaatat tactcatgtg atgtcactga aaatgattgc
      601 gcgctgacgt aacgcttgag gtgctgcata aagcagcgac taaattgaga ttttcctgaa
      661 ttagtgagct gatccgcagc aatattttgt ttatcctgta ttttcagagg gaatggagtg
      721 taacgctctg tattaacaag gagagcatta aaatgggtaa actcacgggc aagacagcac
      781 tgattacggg cgcattgcag ggaattggcg aaggaattgc cagaactttt gcgcgtcatg
      841 gcgcaaacct aatcttgctg gatatctccc ctgagatcga aaagctggcg gacgaactgt
      901 gtggtcgtgg tcatcgctgt acggcggttg tcgccgatgt gcgtgacccg gcgtcggtag
      961 ccgcagctat caagcgcgct aaggaaaaag aagggcgcat tgatattctg gtgaataacg
     1021 caggcgtttg tcgtctgggc agtttccttg atatgagcga tgaagatcgc gatttccata
     1081 ttgatatcaa tataaaaggc gtatggaacg tcacgaaggc ggtgctgccg gagatgattg
     1141 cccgcaaaga tggccggatt gtgatgatgt cttcagtcac tggcgatatg gtggcagacc
     1201 cgggcgaaac ggcgtacgcc ttaacgaaag cggcgattgt tggcctgact aaatcgctgg
     1261 cggtggagta cgcgcagtct ggtattcgcg ttaacgccat ctgccctgga tacgttcgca
     1321 cgccaatggc ggaaagcatt gcccgccagt cgaacccgga agatccagaa tcggtgctga
     1381 ccgaaatggc gaaagcaatc ccgatgcgtc gcctcgccga tccgctggaa gtcggcgaac
     1441 tggcggcctt cctcgcatcg gatgaatcca gctatttaac cggtacacag aatgtgattg
     1501 atggcggcag cacactgccg gagacggtta gcgtcggcat ctgattcacc tctgtttcct
     1561 ccctgcattt gtggggagga tttcgtctta ttcccttttc aacttccaaa tcaccaaacg
     1621 gtatataaaa ccgttactcc tttcacgtcc gttataaata tgatggctat tagaaagtca
     1681 ttaaatttat aagggtgcgc aatggccgtt aacttactga aaaagaactc actcgtgctg
     1741 gttgcttctc tgctactggc gggccatgta caggcaacgg aactgctgaa cagttcttat
     1801 gacgtctccc gcgagctgtt tgccgccctg aacccgccgt ttgagcaaca atgggcaaaa
     1861 gataacggtg gcgacaaact gacgattaaa caagctcatg ccggatcatc aaaacaggcg
     1921 ctggcaattt tgcagggttt aaaagccgac gttgtcactt ataaccaggt gaccgacgta
     1981 caaatcctgc acgataaagg caagctgatc ccagcggact ggcagtcgcg cctgccgaat
     2041 aatagctcgc cgttctactc caccatgggc ttcctggtgc gtaagggcaa cccgaagaat
     2101 atccacgact ggaacgacct ggtacgctct gacgtgaagc tgattttccc gaacccgaaa
     2161 acgtcgggta acgcgcgtta tacctacctt gcggcatggg gcgcagcgga caaagccgac
     2221 ggtggtgata aagccaaaac cgaacagttt atgacccagt tcctgaaaaa cgttgaagtg
     2281 ttcgatactg gcggtcgtgg cgcgaccacc acttttgccg agcgcggcct gggcgatgtg
     2341 ctgatcagct tcgagtcgga agtgaacaac atccgtaaac agtatgaagc gcagggcttt
     2401 gaagtggtga ttccgaaaac caacattctg gcggaattcc cggtggcgtg ggtcgataaa
     2461 aatgtgcagg ccaacggcac ggaaaaagcg gcaaaagcct acctgaactg gctctacagc
     2521 ccgcaggcgc aaaccatcat caccgactat tactaccgcg taaataaccc ggaagtcatg
     2581 gacaaactga aagataaatt cccgcagacc gagctgttcc gcgtggaaga caaatttggc
     2641 tcctggccgg aagtgatgaa aacccacttc actagcggcg gggcgtaagt aatgtttgcc
     2701 gtttcttcaa gacgcgtgct gccgggcttt accttaagcc tcggcaccag tctgctgttt
     2761 gtgtgcctga ttttgctgct gccgctctcc gcgctggtga tgcaactggc ccagatgagc
     2821 tgggcgcagt actgggaggt gatcaccaac ccgcaggtgg tcgcagccta taaagtgacg
     2881 ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt tcggtctgct gatggcgtgg
     2941 atcttaaccc gctatcgctt tccgggtcgc acgctgcttg atgcgctgat ggatttaccc
     3001 tttgcgctgc caacggcagt cgccggttta acgctggcct cgctcttttc cgtgaacggt
     3061 ttttacggtg aatggctggc gaagtttgat atcaaagtca cctatacctg gctggggatt
     3121 gcggtggcga tggcctttac cagcattccg tttgtggtgc gcaccgtgca gccggtgctg
     3181 gaagagttag gcccggaata tgaagaggcg gcagaaacgc tcggcgcaac acgctggcag
     3241 agttttcgca aagtggtgct gccggaacta tctccggcgc tggtggcggg tgtggcgctg
     3301 tcgtttaccc gcagcctcgg tgagtttggc gcggtgattt ttatcgccgg gaatatcgcc
     3361 tggaaaacag aagtgacgtc gctgatgatc tttgttcgct tacaggagtt tgattacccg
     3421 gcggcgagcg cgattgcctc ggtgatcctc gcggcatcgc tgctgttgct gttctcaatt
     3481 aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg gaagtgacac
     3541 aattgaagcg ttatgacgcg cgcccgatta actggggcaa atggtttctg attggcatcg
     3601 ggatgctggt atcggcgttc atcctgctgg tgccgatgat ttatatcttc gtacaggcgt
     3661 tcagcaaagg actgatgccg gtgttacaaa atctggccga tccggacatg ctgcacgcca
     3721 tctggctgac ggtgatgatc gcgctgattg ccgtaccggt aaacctggtg ttcggcattc
     3781 tgctggcctg gctggtgatg cgctttaact tccccggacg ccagctattg ctgacgctgc
     3841 tggacattcc gtttgccgtg tcgccggtgg ttgctggtct ggnnnnnnnn nnctgttcta
     3901 cggctctaac ggcccgctgg gcggctggct ggatgaacat aatctgcaaa ttatgttctc
     3961 ctggccgggt atggtgctgg tcaccatctt cgtgacgtgt ccgtttgtgg tgcgcgaact
     4021 ggtaccggta atgttaagtc agggcagcca ggaagacgaa gcggcgattt tacttggcgc
     4081 gtccggctgg cagatgttcc gtcgcgtcac gctgccgaac atccgctggg cgctgcttta
     4141 cggcgtggtg ctgaccaacg cccgcgcgat tggcgagttt ggcgcggtat cggtggtttc
     4201 cggctcgatt cgtggcgaaa ccctgtcgct gccgttacag attgaattgc tggagcagga
     4261 ctacaacacc gtcggctcct ttaccgccgc cgcgttgttg acgctgatgg cgattatcac
     4321 cctgttttta aagagtatgt tgcagtggcg cctggagaac caggaaaaac gcgcgcagca
     4381 ggaggaacat catgagcatt gagattgcca atattaagaa gtcgtttggt cgcacccagg
     4441 tgctgaacga tatctcactg gatattcctt ctggtcagat ggtcgcgttg ctggggccgt
     4501 ccggttccgg aaaaaccacg ctgctgcgca ttattgccgg gctggagcat caaaccagcg
     4561 ggcatattcg cttccacggt accgacgtga gccgtctgca cgcgcgggac cgtaaagtcg
     4621 gtttcgtgtt ccagcattac gcgctgttcc gtcatatgac ggtgttcgac aatatcgctt
     4681 ttggcctgac ggtgttgccg cggcgtgaac gcccgaatgc ggcggcgatc aaagcgaaag
     4741 tgacaaaatt gctggagatg gtgcagctcg cgcatctggc ggatcgttat ccggcgcagc
     4801 tttccggcgg ccagaaacag cgtgtggcgc ttgcacgcgc gctggctgtg gaaccgcaaa
     4861 ttttgctgct tgatgaaccg tttggcgcgc tggatgcgca ggtgcgtaaa gagctgcgtc
     4921 gctggctgcg tcaactgcat gaagaactga aattcaccag cgtgttcgtg acccacgacc
     4981 aggaagaggc gaccgaagta gctgatcgtg tagtggtgat gagtcagggc aatattgaac
     5041 aagctgacgc accggatcag gtatggcgtg aaccggcgat tggttttgtg tgggggttta
     5101 tgggcgaggt aaaccgcctg cagggaacca ttcgcggcgg gcagttccac gttggcgcac
     5161 atcgctggcc gctgggatac acccccgcgt atcaggggcc ggtggatctc ttcctgcgcc
     5221 cgtgggaagt ggatatcagc cgccgtacca gcctcgattc gccgctacca gtacaggtac
     5281 tggaagccag cccgaaaggt cactacaccc aattagtggt gcagccgctg gggtggtaca
     5341 acgaaccgct gacggtcgtg atgcatggcg acgatgcccc gcagcgtggc gatcgtttat
     5401 acgttggtct gcaacatgcg cggctgtata acggcgatga gcgtattgaa acccgcgatg
     5461 aggaacttgc tctcgcacaa agcgcctgat aggttgagtg aatgttaaac gcccggaggc
     5521 gcttcccgcg atccgggctt tttaatggca aggttttgta acctgtaggt ctgataagac
     5581 gcgtaagcgt cgcatcaggc aacaccacgt atggacagag atcgtgagta cattagaaca
     5641 aacaataggc aatacgcctc tggtgaagtt gcagcgaatg gcgccggata acggcagtga
     5701 agtgtggtta aaactggaag gcaataaccc ggcgggatcg gtaaaagacc gtgcggcgct
     5761 ttcgatgatt gtcgaggcgg aaaagcgcgg cgagattaaa ccgggtgatg tgctgatcga
     5821 agccaccagt ggtaacaccg gcattgcgct ggcaatgatt gcggcgctta aaggctatcg
     5881 catgaaattg ctgatgcccg acaacatgag ccaggaacgc cgtgcggcga tgcgtgccta
     5941 cggtgcagaa ctgattctgg tcaccaaaga acagggcatg gaaggcgcac gcgatctggc
     6001 gcttgaaatg gcgaatcgcg gcgaaggaaa gttgctcgat cagttcaata atcccgataa
     6061 tccttacgcc cattacacca ctaccgggcc ggaaatctgg cagcaaactg gcgggcgtat
     6121 tacccatttt gtctccagca tgggaacaac cggcactatc accggcgtgt cacgctttat
     6181 gcgcgaacaa tccaaaccgg tgaccattgt tggcctgcag ccggaagaag gcagcagtat
     6241 tcccggcatt cgccgctggc ctgcggaata tctgccgggg attttcaatg cttctctggt
     6301 ggatgaggtg ctggatattc atcagcgcga tgcggaaaac accatgcgcg aactggcagt
     6361 gcgggaaggg attttctgcg gcgtaagctc cggcggcgcg gttgccggag cactgcgggt
     6421 ggcaaaagct aaccctggcg cggtggtggt ggcgatcatc tgcgatcgtg gcgatcgcta
     6481 cctttctacc ggggtgtttg gggaagagca ttttagccag ggggcgggga tttaagatta
     6541 ttgcgcaggc accgttttat ccgtctgcgc aattaacgca tgtaaaaaag gctgtgcgtt
     6601 ctcatcgctc ttaccgggca tcttcacgat atacggcttc ccggtaatcg atgacgacga
     6661 ggccaccttg tcaataaact gctcggctgt atcaatgcga ttacgggtat tgcccagctt
     6721 caggcgcaga tgagaaaccg cttcatcgca ggtatgttca tcaccgttgc gcacaaatat
     6781 caaatccttt ttctgtgcta atccctccag catggcgttg atacgggctt cttcatgggc
     6841 cgttaacttc gcataagcgg ggagtgtcat tagcagcgta acgaccagac aaatgatttt
     6901 cttcactgaa ttaccttatc cttgttttgt aatagccgat ttgactgcca gaatcaggta
     6961 aagtttcggc tggaatacga tgaggatcat cggcaaggag aaaaaattaa atgagtagtt
     7021 tgttgttgtt taacgataag agcagggcac tgcaggcgga tatcgtcgcc gtgcagtcgc
     7081 aggtagtgta cggcagcgta ggcaacagca ttgcggtgcc tgctataaaa cagaacggcc
     7141 tgaatgtctt tgccgtgccg acggtattgc tgagcaatac gccgcattat gacactttct
     7201 acggtggtgc gattccggat gaatggttta gcggttattt gcgtgcgctt caggagcgtg
     7261 atgcgctgcg ccaacttcgt gctgtaacca cgggctatat gggaacggca tcgcaaatca
     7321 aaatccttgc cgagtggctg actgcgctac gcaaagacca tcctgaccta ttgatcatgg
     7381 tcgatccggt gattggcgat attgatagcg gaatttatgt caaacctgac cttcccgagg
     7441 cgtatcgaca atatttactg ccgctggcac agggaattac ccccaatatc tttgagctgg
     7501 aaatcctgac cggtaaagat tgccgcgatc tcgacagtgc cattgctgcc gcaaaaagtc
     7561 tgctttcaga cacattaaag tgggtggtga ttaccagcgc ctccggcaat gaagaaaatc
     7621 aggagatgca ggtggtggtg gtcagtgccg acagcgtgaa tgtcatttcc cattcccggg
     7681 ttaaaactga cctgaaaggg actggcgacc tgttttgtgc tcagcttatc agtggattgc
     7741 tgaaagggaa ggcgttaacc gatgcggtgc accgcgcggg gttgcgcgta ctggaagtga
     7801 tgcgctatac ccagcagcat gagagcgatg aattgatttt gccgcctctg gctgaagcat
     7861 aaaaaaatgg cgccgatggg cgccattttt cactgcggca agaattactt cttgatgcgg
     7921 ataaccgggg tttcacccac ggttacgcta ccggacagtt tgatcagttc tttgatttcg
     7981 tccatgttgg agataacaac cggagtcagg gttgacttgg ctttctcttc cagcagcggc
     8041 agatcaaatt caatgacagt atcaccaact ttcacgcgct gaccttcttc agcaatacgc
     8101 ttgaagcctt cgcctttcag ttcaacggtg tcgataccga agtggacgaa cagctcaacg
     8161 ccgctatcag attcgataga gaacgcgtgg ttggtttcaa agattttacc aatggtgccg
     8221 tctacaggcg cgaccatttt gttacccgtt ggtttgatag caatgccatc accaacgatt
     8281 ttttccgcaa aaacgacatc cggcacgtct tcgatattga cgatctcgcc agagagcgga
     8341 gcaatgatct caatagttcc ggtatccttc ttgtcgtcgg aaaccagaga tttcagttta
     8401 tcgaacaaac ccatgatctt ctcctaagca gtaaattggg ccgcatctcg tggattagca
     8461 gattgttttt tcttcaatga acttgttaac cagcgtcatt aactcgtccg ttgtcggttg
     8521 agcaagagcc tgctctgcta acaccttcgc atcttcgaag ttcgtgttac ggataatctt
     8581 cttaatgcgc gggatagaaa tggcgctcat agagaattcg tccagaccca tccccagcaa
     8641 cagaagtgta gcacgttcat cgccagcaag ctcaccacac atgccagtcc atttgccttc
     8701 agcatgagaa gcatcaataa cttgcttgat caagttcagc acggacggtg acattggctg
     8761 gtaaaggtgt gaaatcatat cattaccacg gtcaactgcc agagtgtact gcgttaaatc
     8821 attggtgccg atactaaaga aatcaacttc tttggctaaa tgacgtgcaa ttgttgcggc
     8881 agccggtgtt tccaccatta cgccgatttc aattgactcg tcaaacgctt taccttcgtc
     8941 gcgcagttcc tgtttgtaga tttcgatctc tttgcgcagt gcacgcactt cttcaacaga
     9001 gatgatcatc gggaacatga tgcgcaattt accgaaagca gaggcacgca ggatagcgcg
     9061 gagctgatcg cgcaggatct ctttacgatc catcgcgata cggatagcgc gccagccgag
     9121 gaacgggttc tcttctttcg ggaagttcat gtatggcagc tctttgtcgc cgccgatgtc
     9181 catggtacga acgataaccg cctgagagcc acacgcttca gccactgctt tgtaagcagc
     9241 aaactgttct tcttcagtgg gcagcgcgtc gcggtccatg aacaggaact cagtacgata
     9301 cagaccaacg ccttcagcgc cgttacgctc tgcaccttca acgtcacgaa ccgtaccaat
     9361 gttagcgcac acttctacct ggtgaccgtc cagcgtaata gctggcagat ctttcagttt
     9421 agcaagctct gctttttcag aagccacttg ctcctgaaca gcgcgcattt tatcaataac
     9481 ttcgttggtt ggattgacgt aaacctgatt atttacggca tccagaatca gatagtcgtc
     9541 atttttcacc tgagaggtga cgctaccggt acccacgata gcaggcagtt ccagagaacg
     9601 cgccatgata gaggtgtggg aagtacggcc acccgcgtcg gtgatgaaac ccagcacctt
     9661 cttcaggttc agctgtgcgg tttcggacgg cgtcaggtca gcggcaacca gaatgacttc
     9721 atcctgaatg gcgctcaggt cgataatctt caggcccagg atgttgcgca gcaggcgctt
     9781 accgatatca cgtacgtcag ccgcacgttc tttcaggtat tcatcatcca gctcttccag
     9841 ggcagaagcc tgaccttcga taacttcatg agcagctgcg tcagctgtca tgtgcttatc
     9901 tttaatcagg gctatgattt cctgctccag ctcctcatct tcgagcagca taatatgccc
     9961 ttcaaagatg gcttcttttt cttcaccgaa cgtttcacca gctttcgttt tgatcgtttc
    10021 cagctgggct gatgccttgg cacgaccgct
//
LOCUS       DAWDRC010000062         9988 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3431_length_9988_cov_2.100574, whole genome shotgun sequence.
ACCESSION   DAWDRC010000062 DAWDRC010000000
VERSION     DAWDRC010000062.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3431_length_9988_cov_2.100574"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1642..1651
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8301..8310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2400 a         2595 c         2688 g         2285 t
ORIGIN      
        1 gcattcgtca gcaacaccgg caccgtcgca ctgctgctgc ccatcgcagt cagccttgcc
       61 gcaagtttcc acatgaatcc gagccgcctg ctgatgccga tcgctttcgc cagcagcatg
      121 ggcggcatga tgacattaat cggcacaccc cccaacctcg ttatacaaaa tacgctgacc
      181 gaagccggat ttcaaccgct cgaattcttc tcctttcttc cggtaggttt aatttgtgtc
      241 ggggtcggaa ccctcgtact gattcctctg agcaaatatt ttctttcgaa aacagggaca
      301 aaagagaaac aacgcggtta caaaaacaaa tcgctgaacg atctggctgc ggaatatcag
      361 ttgggcgacc atcttttccg gatgcacgta gcaccccatt ccccgctgat tggcaaaaca
      421 gccgcagaag ccgacatccg caaccgctac gacctgaaca tcctcgaaat aagaaggatt
      481 accgttcccc agagccgttt tttcaaaacg atcagccagc gaacagcccc gaatactgtt
      541 ttactgccag atgacctcct ttatgtgatg ggggatttcg acaaagtgca acatttcgct
      601 gaaacctacc gtttggaact gctggacaca cacatgcccg aagagaggtc cgcaataaac
      661 cgcaattcac tggactttta cgacatcggt atcgcagaga tcgtcctgat gcctacttcg
      721 ggcctgatca accgtaccat aggggaatcg gggttccgag acaaatacca cgtcaacatc
      781 ctcggcattc ggcgtaaacg cgaatacatc ctcaataacc tgaaagacga gaagatgcac
      841 agcggagatg tactgctggt ccagggcagc tggaatgcca tcggccattt gagccaggaa
      901 aactcggact gggtcgtatt gggacaaccg ctaaatgaag cggccaaagt caccctcgac
      961 tacaaagctc cattagcagg tatcatcatg ctcctgatga ttatggcgat ggtgttcgac
     1021 ttcatcccga tcgccccggt tacggcgatt atgatcgctg cggtattgat ggtactgacg
     1081 ggttgtttcc gcaacgtgga agctgcatac aaaaccatca actgggagag tatcgtactg
     1141 attgcggcca tgctgccgat gtcgatggcc ctggaaaaga ccggcgtatc cgaatacata
     1201 tccaacagtt tggtcagcgg cctgggcgtt tacggcccga tagccctgtt ggccggcatc
     1261 tatttcacca catctttact gaccatgttt atcagcaata ccgccacggc cgtacttctg
     1321 gctcccatag ccctgcaatc ggcaatacaa atcggaacca gcccatatcc gttccttttt
     1381 gcggtaaccg tcggagccag tatgtgtttt gcctcgccgt tctccacgcc gcccaatgca
     1441 ctggtcatgc cggccggaca atacactttc atggattaca tcaaagtcgg attgccattg
     1501 caaattctga tcggcatagt gatgatactg acacttcccc tgctcttccc cttttaaccg
     1561 gcacgggccg gtcatccctt gtccgctccg aacaaccggc ttcgctgcaa aggatcgctt
     1621 ttattacata ctgaagcttt cnnnnnnnnn naagaaacac agcaaacaaa atacccggca
     1681 gtatctctgc cgggtatttc gttgaggttc acttcgtcca caagaactat gcacgtttgg
     1741 cgttaaattc gtccagcgcc tcgtagaaag catcgatatt agccgccgga acttcggggg
     1801 gtgtatcgca tcccgatgaa aggatgaagt ttttgtaatc ttttgtccgt tcgagcagat
     1861 cggacacagc cgttttcatc tcctgtgccg tggctacttt gaacatggct acgggatcga
     1921 tatttcccat cgccaacacg ccttccggcg tatcctcgag agccttgacc atatcgatct
     1981 tgttaccgaa atggagtgcc caggcaccgg ccgccgccat cgactgtgta cagtgaccgg
     2041 tattaccgca attgtgcagg acgacgatga aattttcatc ctgaaccgca tccacgatct
     2101 ctttcaggta cggagtcgca aacttcaggc agtcgtcatt cgacaacagt cctgccgccg
     2161 gctcggcgag cactacaccg ttcgctccga ctttcttcag ctcgaggcag tagttcttga
     2221 tgaatgcggt acatttcttc agcaacaact cgatcgtatc cggctctacg tagcaggcca
     2281 tcataatctc ggtcatgtcg aacaggcggc ccgccagcga aaaaggaccg atgcatccgg
     2341 ccagagtcgg cttatccgtg atgttttcgg cagccagttt attggccagg acataaacca
     2401 ggcactgcct ggcgatatga tttttttcga tcagggcgat gcccagcact taatggtctg
     2461 gatggggcgt tacatcatct accacaccgg aagcgccacg aaaactgaca acggaatgcg
     2521 cgcagtcagt ctgcaacaac ttatgacatg gaaggacacc cgatggatac ccaacgattc
     2581 caatcccaat ttcattggca tttatcgttt aaattttctg gcgcgatagc agcgtgcttg
     2641 tccctgtctc ttgtgggaac agggcttgct aatgctgatg attcgcttcc ttccagtaac
     2701 tatgtgccgc ccgccggggg aacattcttt ttgctcgctg acagcagttt tagcagcagt
     2761 gaagaggcga aagtgcgact ggaagcgccg gggcgtgatt atcggcgcta tcagatggaa
     2821 gagtacagcg gcgtggacgt tcgcctgtat cgtattcctg acccgatggc ttttttgcgc
     2881 cagcagaaaa acctgcatcg cattgtggtg caaccgcaat atctgggcga cggactgaac
     2941 aatacgctga cctggctgtg ggataactgg tacggcaaat ctcgccgcgt gatgcagcgt
     3001 actttctctt ctcagtcacg gcagaatgtg actcaggcgt tacccgaatt acagctcggt
     3061 aatgccatta ttaaaccttc ccgttatgta cagaacaacc agttttcacc gctgaaaaaa
     3121 tatccactag tggaacagtt ccgttaccca ctatggcagg ctaaaccggt cgagccgcaa
     3181 caaggggtaa aactggaagg cgcatccagc aatttcattt cgccgcagcc gggcaacatc
     3241 tatattcctc tcggcaaaca agagccggga ctgtacctcg tcgaggcgat ggtgggtggg
     3301 tatcgggcga cgacggtggt gtttgtttcc gataccgtgg cgcttagcaa agtgtcaggt
     3361 aacgagcttc tggtgtggac cgcggggaaa aaacagggtg aagcgaagcc cggctcagag
     3421 atcttgtgga ctgacggtct tggcgtgatg acccgcggtg tgaccgatgg cagcggtacc
     3481 ttgcagttac aacatatatc gccagaacgt tcatatattc tgggtaagga tgctgaaggc
     3541 ggcgtttttg tctccgagaa ctttttctac gaaagcgaaa tctacaacac ccgcttgtat
     3601 atctttaccg atcgcccgct atatcgcgca ggcgatcgtg tcgatgttaa agtgatgggc
     3661 cgcgagttcc acgatccgtt gcattcatcc cctatcgtca gcgcaccggc gaaactttca
     3721 gtgctggatg ctaatggcag cctgttgcaa accgtcaatg tcacgctgga tgcgcgtaat
     3781 ggcgggcagg ggagtttccg cctgccagaa aatgccgtcg ccggaggtta tgagttacgt
     3841 cttgcttacc gcaaacaggt ctatagcagc agttttcgcg tggcaaacta catcaaaccc
     3901 cattttgaga ttggtttagc tctcgataaa aaagagttca aaactggcga agcggtcagc
     3961 ggcaaactgc aactgctcta cccggatggc gagccggtaa aaaatgcccg cgtgcagtta
     4021 agtttgcgcg ctcagcaatt atcaatggtt ggtaacgatt tgcgttatgc aggacgtttc
     4081 ccggtgtcgc tggaaggcag tgaaacggtt tccgacgata atggtcatgt ggcgttgaat
     4141 ctccccgccg ccgataaacc aagccgttat ttgttaactg tctccgccag tgacggcgcg
     4201 gcgtatcgtg tcaccaccac caaagagatc ctcattgaac gcggtctggc gcattactct
     4261 ttaagtactg ctgcacaata cagtaatagc ggtgaatcgg ttgtgttccg ttatgccgcg
     4321 ctggaatctt caaaacaggt tcctgttact tatgaatggt tgcgtctcga agaccgcacg
     4381 agccacagcg gagagctaca gtcaggcggc aaatccttta ccgtcaattt cgctaaacct
     4441 ggcaactaca atctgacatt acgcgataaa gacggcttaa ttctcgccgg gttaagccat
     4501 gcagtcagcg gtaagggcag cacgtcgcat actggtacgg tagatatcgt ggcagataaa
     4561 acactgtatc agcctggcga aaccgcgaag atgctgatta ccttcccgga gccaattgac
     4621 gaagcattat tgacgctgga acgcgatcgc gtggaacagc agtcgctgct atcgcatccg
     4681 gcaaactggc taacgctaca acgtttaaac gatacccagt atgaagcccg cgtaccggtg
     4741 agcaattcct ttgcgcctaa catcactttt tcggtgctgt atacccgtaa cggtcagtac
     4801 agttttcaga acgccgggat taaagttgcc gttccccagc tggatatccg ggtgaaaacg
     4861 gacaaaaccc attaccagcc tggtgaactg gtcaatgtcg aattaacctc gtcgctgaaa
     4921 ggtaaacctg tttctgcgca gttaacggta ggcgtggtcg atgaaatgat ctacgcgctg
     4981 caaccggaaa tcgcgccgaa tatcggcaaa tttttctatc cgctggggcg taacaatgtg
     5041 cgtaccagct ccagtctgtc gtttatcagc tacgatcagg cgctctccag cgagccggtt
     5101 gcgcctggcg cgactaaccg cagcgagcgg cgagtaaaaa tgctggaacg tccacggcgt
     5161 gaagaggtgg ataccgcggc ctggatgccg tcactcacaa ccgataaaca aggcaaagcg
     5221 tatttcacgt tcctgatgcc tgattcgtta acccgctggc gcatcaccgc gcgtgggatg
     5281 aacggcgacg ggctggtcgg gcaggggcgt gcttatctgc gttcggaaaa aagtctctac
     5341 atgaaatgga gtatgccaac ggtgtatcgc atgggcgaca aaccggcggc aggactgttt
     5401 atcttcagtc agcaggataa cgaaccggtg gcgctggtga ctaaatttgc aggtgctgag
     5461 atgcgccaga cgctgacgct gcacaaaggg gcgaattata tttcgctgac gcagaatatt
     5521 cagcaatctg gcttgttaag tgcagaactg caacaaaatg ggcaagtaca ggacagcatt
     5581 agcacaaaac tgtcttttgt ggataacagc tggcccgttg aacaacagaa aaacgtcatg
     5641 ctcggcggtg gcgataatgc gctgatgttg cccgagcagg cgagcaatat ccggctacaa
     5701 agtagcgaaa cgccgcagga gattttccgt aacaatcttg atgcattagt cgatgaaccg
     5761 tggggtggcg tgatcaacac cggtagccgt ctgatcccgc tcagtctcgc ctggcgttcg
     5821 cttgccgatc atcaaagtgc cgccgctaac gacattcgtc agatgattca ggataaccgt
     5881 ctgcggctga tgcaactggc ggggcccgga gcgcgcttta cctggtgggg tgaagatggc
     5941 aatggcgacg ccttcctcac ggcatgggca tggtacgccg actggcaggc cagccaggcg
     6001 ctcggcgtaa cgcagcaacc ggaatactgg caacatatgc tcgacagtta tgccgagcag
     6061 gcggacaaca tgccgttatt gcatcgggcg ctggtgctgg cgtgggcaca ggagatgaat
     6121 ctgccgtgca aaacgttgtt gaaagggttg gatgaagcta tcgcccggcg cggaactaaa
     6181 actgaagatt tctctgagga agacaccagc gatatcaatg acagcctgat cctcgataca
     6241 cctgaatctc cactggcaga tgcggtggca aacgtcttaa ccatgacgtt gctgaaaaaa
     6301 gcgcagttga agtccactgt gatgccacag gttcagcaat atgcgtggga taaagcagca
     6361 aacagcaatc agccgctggc gcacacggtt gtgctgctca atagtggagg cgacgctacc
     6421 caggcggccg ctattttaag tggtttgacc gctgagcaat ccactattga gcgcgcgctg
     6481 gccatgaact ggctggcgaa atatatggcg acgatgcctc cagttgtgtt gcctgcgcct
     6541 gcgggcgcat gggctaaaca taagttaact ggagggggcg aagactggcg ttgggttggt
     6601 cagggtgtgc cggacattct ctcttttggt gacgaattat cgccgcaaaa tgtgcaggtc
     6661 cgctggcgtg aaccggcaaa aacggctcaa caaagtaaca ttccggtgac cgttgaacgc
     6721 cagttgtatc ggcttatccc cggtgaagaa gagatgagct ttactctgct gccggtgacc
     6781 agcaatgaga ttgacagcga tgcgctgtat ctcgatgaaa ttacgcttac cagcgagcag
     6841 gatgccgttc tgcgctacgg tcaggtggaa gtaccgctcc cgccaggggc tgacgttgag
     6901 cggacaacgt ggggcatttc ggtcaataaa cctaacgctg gaaaacagca ggggcaattg
     6961 ctggaaaaag cgcgcaatga aatgggcgaa ctggcctata gggtgccggt gaaagaactg
     7021 acgggaacgg tcactttccg ccatttgctg cgcttctcgc aaaaagggca attcgttctg
     7081 cctcctgctc gttatgtgcg ttcctatgca cctgcacagc aaagtgttgc ggcagggagt
     7141 gaatggaccg ggatgcaggt gaaataagtg aactggcgca gaatcgtctg gctattggcg
     7201 ttggttactc tgccaacgct tgcggaggag acgccgttac aactggtgct gcgcggtgcg
     7261 cagcacgatc aactgtatca gatgtcgtca tcaggcgtga ccaaagtctc agcattacct
     7321 gacacgctca ccacgccgtt agggagttta tggaaactgt atgtttacgc ctggctggag
     7381 gatacccatc aaccggagca gccctaccag tgtcgcggaa actccccgga agaggtttat
     7441 tgttgtcagg ctggggagag cataacgcgc gataccgcgc tggtacgctc ctgtggactc
     7501 tattttgcgc cgcaacggtt gcatattggt gcagatatgt gggggcaata ctggcaacag
     7561 cgtcaggcac ctgcgtggtt agcgtctctt ccgacgctga aaccagaaac ctcggtaacg
     7621 gttaaatcgc tgcttgattc attagcgacg ttacccgcgc aaaacaaagc tcaggaagtc
     7681 ttactggatg tggtgctgga cgaggcgaaa atcggtgtcg catccatgct cggtagtcgg
     7741 gtacgggtga aaacctggag ctggtttgcc gatgataaac aggaaatccg tcagggtggt
     7801 tttgctggct ggttaaccga tggtacaccg ctgtgggcta ctggcagtgg caccagtaaa
     7861 acggtactga cccgttatgc gacggcgctt aatcgggttt tgccagtgcc gacgcaagtt
     7921 gccagtgggc aatgtgtgct ggtggatctg tttgctcgtt atccgctgaa gaaagttact
     7981 gaggaaaaga gtacgacggc attcaaacca ggcgtgctca atgggcgcta tcgtgtgacg
     8041 tttgccaatg gtaaccacat gacatttgtc agccacggtg agacaacgtt actgacggta
     8101 aagggcaaac tgaaattaca gtcgcatctc gatcgtgaag agtacgttgc cagagttctg
     8161 gatcgcgagg cgaaaagtac gcctcctgag gcagcgaaag ccatgaccgt ggcgattcgc
     8221 acgtatttac agcaaaacgc cgatcgcgat ggtgattgcc tgagcatccc ggacagtagc
     8281 gctacccagc gcgtttcagc nnnnnnnnnn taggcgcgcg gacgatgacc gcctggacgc
     8341 aggatctcat ctatgcgggc gatccggttc attatcatgg cagccgggtt accgaaggga
     8401 cgctttcctg gcggcacgct actgcccagg cggggcaggg agagcgttat gatcagatac
     8461 ttgcgtttgc ttatcccgac aacaacctta gccgctgggg agcgccacgc tcaacctgcc
     8521 agttattacc caaagcgaaa gcctggctgg cgaaaaaaat gccgcagtgg cggcgtatat
     8581 tacaaggtga gacgggatac aacgaaccag acgtgtttgc ggtctgtcgt ttagtctcag
     8641 gtttccccta taccgatcgg cagcagaaac ggctgtttat tcgtaatttc tttacgcttc
     8701 aggatcggct ggatttaacc catgagtatc tgcacctggc ctttgatggt tatcccaccg
     8761 ggcttgatga gaactatatc gaaacgctga cccgacaatt attaatggac taatgctatg
     8821 cgaaagattt ttcttccgtt gttactggtg gcgctttcgc ctgtcgctca cagtgaaggt
     8881 gtgcaggaag tcgagattga tgcaccgctc tctggctggc atccggcaga gggtgaagat
     8941 gccagttttt cacaaacgat caattacccg gcgtcgtccg tcaacatggc agatgatcaa
     9001 aatatctcag cgcagatccg cggcaaaata aagaattatg ctgcggcggg taaagttcag
     9061 cagggccggc tggtggtcaa cggtgccagt atgccgcaga gaattgaatc agatggttct
     9121 tttgcacgcc cttatatttt cactgaaggc agcaacagcg tgcaggtcat cagcccagag
     9181 gggcaaagcc gacaaaaaat gcagttttac tcaacgccgg gtgctggggc gattcgcgca
     9241 cgtttacggc tggttctctc gtgggatacg gacaataccg acctcgatct tcatgtcgtt
     9301 acgcctgacg gtgaacatgc ctggtacggt aacaccgtgc tgaaaaacag tggtgcactg
     9361 gatatggatg tcacgacagg gtacggcccc gagattttcg ccatgccagc gccagttcac
     9421 ggccgttatc aggtgtatat caactactat ggcggacgca gcgaaacgga attaaccacc
     9481 gcccaactga cgctgatcac cgatgaagga tcggtcaatg agaaacagga aacatttatt
     9541 gtaccgatgc gtaatgctgg cgagctgacg ctggtgaaaa gttttgactg gtgattaatg
     9601 gggttacctt cgccgtcgat gttcaagaaa acgcccgata actttcgcta tcgggcgttt
     9661 ttattggtta attcaaccgc tcaatcacca tcgcaattcc ctggccgcca ccaatgcaca
     9721 gtgttgccag ccccagcgtt ttatcgcgtg cctgcattgc atgtaatagt gtgaccagaa
     9781 tacgagcacc actggcaccg ataggatgcc ccagcgcgat ggccccgccg ttgacattca
     9841 ctttctcagg atcaaagccc agggttttcc caacggcaag gaactgtgca gcaaatgctt
     9901 cattagcctc aatgagatca atatccgcca gttgcagccc cgccagttgt aacgcttttt
     9961 gcgtggcagg tactggcccc atacccat
//
LOCUS       DAWDRC010000063         9910 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3465_length_9910_cov_2.657331, whole genome shotgun sequence.
ACCESSION   DAWDRC010000063 DAWDRC010000000
VERSION     DAWDRC010000063.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3465_length_9910_cov_2.657331"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3778..3787
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7187..7196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2614 a         2438 c         2283 g         2555 t
ORIGIN      
        1 aaactcacca catggtcatt taagccagga ataaattagc ttcccgttca tcatttttgt
       61 gatcttgcac acatctttga gataacgcac tttcttcacc agtacgttgc tcgctttgtc
      121 agcagaagat tgcaacattc tgtcagaagg ccgcgaatcc cccgttttgt cgtggtaatg
      181 tatgcacttt gtgttagacg ggataggttt ttaagatgga aaaactgcgg gtaggaatcg
      241 tttttggtgg taaatcagcg gaacatgaag tgtctctgca atcggcaaaa aacattgtcg
      301 atgccattga taaaagtcgc ttcgatgttg tgctgctggg cattgataaa caagggcaat
      361 ggcacgtcag cgatgccagc aattatctgc taaatgcaga cgatcctgcc catattgcgt
      421 tgcgcccttc ggcgaccagc cttgcgcagg tgccaggtaa acatgagcat cagcttattg
      481 acgcacaaaa cggtcagccg ttgccgacgg tggatgtcat tttcccgatt gtccacggta
      541 cgctgggcga agatggttcc ttgcagggaa tgctgcgggt cgccaattta ccgtttgtag
      601 gttctgatgt tctggcttca gcggcctgta tggataaaga tgtcaccaaa cgtctgctgc
      661 gcgatgccgg gctgaacatt gcgccattta ttaccctgac gcgcgctaat cgtcacaaca
      721 tcagttttgc cgaagtggag tctaaactgg ggttaccgct gtttgtaaaa ccggctaatc
      781 agggctcttc tgttggtgtc agcaaagtaa ccagtgaaga acagtacaca attgccgtcg
      841 atctggcgtt cgagttcgat cataaagtga tcgttgagca agggatcaaa ggtcgtgaaa
      901 tcgaatgcgc agttcttggc aacgataacc cgcaagcaag cacctgtggc gagatcgtac
      961 tcaccagcga tttctatgcc tacgacacca agtacattga cgaagatggc gcgaaagtgg
     1021 tagttccggc agccattgcg ccagaaatca acgataagat ccgggcgatt gccgttcagg
     1081 cttatcagac gttgggatgc gcaggcatgg cgcgtgtaga cgtgttttta accccagaga
     1141 acgaagtggt gatcaacgag atcaacaccc tgcctggctt caccaacatc agtatgtatc
     1201 cgaagctgtg gcaagccagc ggtctgggtt acaccgacct gatcacgcgt ttgattgagc
     1261 tggcgctgga gcgtcacgct gcggataacg cactgaaaac cacaatgtaa tattcaaacg
     1321 cccggtaata ttcttattgg gcgttttcag tttcttcatc gcgacgacga ataatcaatc
     1381 ccgccagcca gaaactaatc acccacgtca ccagaccgac ggcataggtc tgccaacctt
     1441 ttgcttcaaa tcccagtaac cccaccacgc cattaagaat gaatataagg ccaatggcga
     1501 acgcatagta gtgccagtca cggcggattt ttacaggcag attcatggca gctccgttaa
     1561 cgaaaaacaa catcttttca tattctgtaa caactgtctg tggtgtagac aacactttcg
     1621 caattgttaa taaatttaac ttaaggttgc tattttgtga ctcacaacga agattcacgc
     1681 ttcgggaata ttcccaacat caatttacca tgattctgat attgacaatc taaatgacct
     1741 gtcagacttg ccataagccg ctggtatcac accagaagag aagcagatga cttccggagg
     1801 tttttatggc tgatttcacc ctgtcaaaat cgctgtttag cggaaaatat cgcaatgcct
     1861 cttcaacgcc tggcaatatt gcctatgcgt tgtttgtgct gttttgcttt tgggctgggg
     1921 cgcaattgct gaacctgcta gtgcatgcgc ccggcgtcta tgagcattta atgcaggtcc
     1981 aggaaacagg tcgcccacgg gtggaaattg gtttaggtgt cggcaccatt tttgggctga
     2041 tcccgttttt agtaggctgc ctcatttttg cagtagtggc gctatggctg cactggcgac
     2101 atcgccgcca gtaagattta cgttgccaga tgcggcctaa aacgccttat ctggcgtgac
     2161 attcctgttt gatcactgtt tcatacaacg cgcccgccgt tcgtctacgc gcttcgcaaa
     2221 ccaggccgta gtcagattgc gggtgatttt cgggctttcc agttgaatgc caggcaacat
     2281 ttcgcggggt agtgatttac ccgttttcgc ttcggcaagt tgataaactt tcttgtacag
     2341 cgccgtctcc tcaaaagaga agctgtcccc tttctctaac tggcggcgaa tttcactgtc
     2401 gctcatcccc agttttcccg ccagtttgcg cgttgccagt tcggttttcc cgggctcttt
     2461 actgtcgtaa cgaattaaat caccgtccag tgccagcttc acgccgctgg ccttactgac
     2521 cgcgttctga aacgcggcat tacggctggc gtaccagcca gcgttaaaat cagcaaaacg
     2581 gtatatcggc gcgctatagc tagcgggata attcagtaaa tggtaagtac caaaccacaa
     2641 cccgccgcga cgactgaaga cttcctgacg gactgtaccg tccattttcc acggataccc
     2701 tttggtatgc tgttcggcaa aagcaatact gacctgcatc ggcccaccgg tgcgcaccgg
     2761 attgagcgaa ccaaacagcg tctgccccat tggcaccctg ctgatcagat cgtcaaaaat
     2821 cgcgctcaat tgcttttcag tacgcaccga atctaaccgt tcgctatagc ttttgccgtt
     2881 tggcgatttg atttttagcg cggtatgcac cagaaaagca ggaatgtgca tccgttcagc
     2941 acgacggtca atcccttgcc aggcgatctt gcttaagccc ggtacggccg gaactgcctg
     3001 ataatttgac tcctgctgcg ccaccgccag caccgagcag acgttttcca gcgtcggtgc
     3061 cagcccctga ctttcaaacg tggtggcgag atctttcgcc caggcatcgc ggtctttcac
     3121 gctcgcaggc atcttctgcc gcacgacgct cgccacatct atcgcttttt cgcccttttt
     3181 caacggttgc ggagcctgac tactacaccc cgctaaaacc agaaccgcca acaatgacag
     3241 cgaagataga tgattgacgc gcgacattac ttctcctttt tagctcaagg tatgggttac
     3301 ttcctgaatt ttgtcaccat ccagctcatg ttcaaagctg cgcagacgtt tgtagataga
     3361 catcaactca accagtgtgg tccatgagtt aatcaggtac tggaaagctc cgcgaacctg
     3421 accaaaaacg ttggtaatct gcgtcatcag gccgagcgta atcgtaccgg caacaattga
     3481 cggaaacagc aagaacaaac cgaaaacgtt atcgacctgc aaatagagaa tgcgggcgat
     3541 gttgaaatac atatagtgaa aatagaggcg aaaatagttt ttccgtacgg cgctaaacag
     3601 ctcgcgtacc gtaggcggcg tcgcgcgcgt ggcatcgtct tcaccataaa ccagctcttt
     3661 acggtaggca gcctctacac gctggttttt aaactccagc cccggcagtt tgatccctac
     3721 cactgccagc aatccggtcc ccatcagcga ccagacgatt gcggcaatca ccagaccnnn
     3781 nnnnnnnacg ctgcgcggcc ccttcgatat gacgcagttg ttgccagttc gccatgtaat
     3841 attcgttcat cgccgtacgc cagcggaaca cgtagtgact gacaaagaag ttgttcagca
     3901 cactgatcac caccgcgatc agcgcaatcc ccagaaagac gcccacttcg cggtaaaatt
     3961 gttcgatggt gactttatgc ggcgaactta gcgccgtttg aatcagatca tagaacggcg
     4021 cataccaggc gttgacggca accccgactt ccaccaaaaa ccaggtgacg aagataatca
     4081 gcgcagtacc gagtattgac cagtattgcc aacggtgcgg gctatagata aaccagaaaa
     4141 atgcaaaaag tcctacgcaa acaatgtagt aagcgtaaaa aatcaggaaa tccaacgacc
     4201 agaaacgcgc ggcgctaatc gggatctgcc cggaagcgcc ggtgatacgc gccacccaat
     4261 cacccccacc gacttgccag aagataacgg caatcaatgc ccaaacaaag gccgagagaa
     4321 aaaacgttcc cggctttggg aaaaaagact taaacatatg cactcctgct aacttcttat
     4381 cgttttaaca actgctgtgt attaaccgca tgaccgctac aaagcgtcag ccaaatgaaa
     4441 ctgcccattg tgacccatag caaggcgctt agttcgccag cctgaccata agaattgttt
     4501 cgacgggttt cacaaaccaa agattgttta cataagcgga ttaacacgcg caggatcaca
     4561 aatgcaggca ttggtgagca ggaaaaagat cgtgtttacg cgccgttacg tttttaacac
     4621 tattattgca ctaattcgcc ctttgcaatc catcaatgag tagtataaat acgctcagtt
     4681 accttcattc aatctatgga caccaccgtt gaaacgtagt ctgctttttt ctgccgtgct
     4741 gtatgcggcg tcattgacct ccgtccatgc ggctcagccg atcactgaac cggagtttgc
     4801 ctctgatatt gtcgatcgtt atgccgatca tattttttac ggcagcggtg ccacggggat
     4861 ggcgctggtt gttatcgacg gtaaccagcg cgtctttcgc agttatggcg aaacgcgacc
     4921 tggtaataac gttcgcccgc agctggattc cgtcatacgt attgcttccc tcaccaagtt
     4981 aatgaccagt gagatgctgg tgaaattgct cgaccagggg accgtgaagt taaacgatcc
     5041 gctaagtaaa tatgctccgc caggcgcacg cgtaccgacc tacaatggga cgcccattac
     5101 gctggtgaat ctggcaaccc ataccagcgc cctgccccgt gaacagcccg gtggcgcggc
     5161 acatcgtccg gtatttgtct ggccaacgcg cgagcaacgc tggaaatacc tttccactgc
     5221 caaactaaaa gccgcgccgg gcagccaggc cgcgtactcc aacctcgcat tcgatctgct
     5281 ggcagatgca ctggcgaatg cgtccggcaa gccttatacc cagttgtttg aagagcagat
     5341 tacccgtccg ttggggatga aagacaccac ctacaccccc tcaccggatc agtgccgccg
     5401 tttaatggtt gctgagcgcg gtgccagtcc gtgcaataac acgctggcgg caattggcag
     5461 cggcggcgtc tattccacgc caggcgatat gatgcgctgg atgcagcagt atttgtcatc
     5521 tgatttttat cagcgcagta accaggctga tcgcatgcag acgttgattt atcagcgcgc
     5581 gcagtttaca aaagtgattg gcatggatgt ccccggcaaa gcggatgcgc ttggtttagg
     5641 ctgggtatat atggccccga aagagggtcg tccggggatt attcagaaga caggtggtgg
     5701 tggcggtttc attacctata tggcgatgat cccgcagaag aatattggcg cgtttgtggt
     5761 ggtcactcgt tcgccgttga cgcgctttaa aaatatgagc gatggcatca atgacctagt
     5821 gaccgagcta agcgggaata aaccgttggt tatccccgca tcctgattaa tactctgaag
     5881 gcaaacggtt gatagcgacc agtttgcctt tattcatttc gatatagccg cctttacgca
     5941 aagcagcgag aacttccgca acgacagaac gcgatatacg tgtacgttga tggatataat
     6001 tcatcacgcc aatacgtgat cgcaattctt cattccagtc aatcattgat aataacgtcg
     6061 cgcggatttg ttcgtaggaa ttatgcccaa tgagctgaac gtcgcgtaat tccagaattc
     6121 tattttgcca ggctaaccag taaaaagcgt ctcgccagag ttgattttgt tcaataagag
     6181 taatggtttg tttggctggc aaatgatatc ccgtacagtt tccttctgat attaatttgt
     6241 atggtatatc gtttttcatt aaaccatcag ccagccccat aatataagga gcctgtgtaa
     6301 taccgataag tacgttttct tctctacgca aagagataac gccctctaaa ataacgaaag
     6361 tatcttcgct attgacatca ctggaaaata taacttgctt ttcattatta aactcgaagc
     6421 gcgtaccgta tctggacaaa catttatcga gcttaccaaa ttcctgaaga ggtttaacta
     6481 cagataacat ttgcgcgtcc tttgcagtaa tgcccgtcaa atccttgacg ggcattattt
     6541 agattaaatt acctatttct tcggagtgaa gaatattacc aggtatattt aacacccaca
     6601 ttcgcggacc agtcttgatc tacgtcacca ccaccgaggt agttagcatc ggtataggcg
     6661 ctgaagttct tggtgaagct gaactgagta cccagaccaa cacgtaccgc agacccttca
     6721 gtaccgttat cgatggaatc accgttcaca tcgttatcgt tgttagagtc gtcgtagacg
     6781 taagccagtt tgaagtacgg agtcagagcc tggtcttcgc tgtaggtgaa ggtataacct
     6841 gcatctacac ccagttcata acgcatgctg tcgtaagact gaccatcaac tttcatgtcg
     6901 ttgctcagct ggtaatcatc accagactgg aacagaccag aaacgctgcc gtaaggagtc
     6961 acgtaacctg catcacccag tttgaagtcg taaccggctt tcaaaccaaa gccccaagcg
     7021 tcggagttgg tgctgccgtc aacgtaagta ccgttgctca tggttgcaga caggtcgttg
     7081 ttgaagtgag agtagctcaa gctaccgtca acaaagacgt tgttcgcgaa gtgagcagaa
     7141 gagtagatgt aggcagtctg gctgtcttga tccacctgac cagaacnnnn nnnnnnactt
     7201 agcgttgtta ccgtcaattt tggtatcaac accgaccatg atgccgttaa catcctgatc
     7261 atagttgatg gtgccgttgt cgccgttgaa gttaccaccg aagtagctta cccatgcgcc
     7321 gccgttatca gccagaccat gacgagagtt ggtcagacga gtaccaacgg tgtcttgttc
     7381 caggttccag atattggtgt tcgcagacgg gatgctcagc gccatgttag cgtagtcggt
     7441 caactcggtc tgatgcagaa caacggtgtt accacgctgc tcagcctgat aggtgtatgc
     7501 acccaggtca gctttgttag cagcagagaa ggtcgcgttg gtgttgacgt cgtttacata
     7561 gataatttct ttatctttgt aatcagcgat tgcgccagcg ccagttgcgt tgtcgatacg
     7621 aactttgtag ttaccggttg ccgcaacaac gttgtctaca gaactgttat ccagttcagt
     7681 accgttgtct acgtcgccgt taccgttgat agccagatga ccatcagagt tcatagcaac
     7741 aacaccgtag ccgtagttag cctgagcggt atcccaagta cggtcgttca ccaggtcagc
     7801 gttcagaaca taatcgctgc tgttgatatc gaacacacca gaaactacat tgccgttgtg
     7861 gtcagcgatg ttgccgctag tcagttccag tttgtcagta cggaaagctt cagtatcaaa
     7921 ctgatcaacg ttcacatcca gtacaccacc gttagttacg gtgatggtat tggcatacag
     7981 gttagaatag ttattccagc cagtagattc ggttaaatta acggtactgt agctatcaac
     8041 agtcaggtgg tctgtagcaa ccaaaccatc ttcaccaata ttcagtgagg agccgttggt
     8101 taaaccaatg gtatctgaga ccagagagga atcagtaaca tttacctgag agccattatt
     8161 tacagctaat gtatcaatta ctgaactatt aacggtattc cattctgaac cattgttcag
     8221 tgctacgtta aatacgccgc tctggaatac cttgttgtca ataatacgac cccaatcgtt
     8281 agcttcgata gtggcaccag gggtcaggct gttcggcgca acatcataca tatctgccgg
     8341 agcaattact tcagcactca tgtttgctga gccaacccac ttactaccgt tgttcagagt
     8401 aatgttcagt tcatcaacgt tctgagcatc atctacccaa ccaccgttgg tatcaaggac
     8461 accgtcacca ttgctgtcat ggccggttga atagaagaat acgccatcgt tattccagtg
     8521 gcttgtgaac tcgacatcac ccaacagggt tgaatggtca aagtttacat tgtttttcat
     8581 ggaataattt gagccagcat tgtcagagaa tgacagagct acgtcatttt cgccaccatt
     8641 accagcgtaa tcacttggtt cagcagattt accgaagaag ccattgcttt cttccagcgg
     8701 agtctgtgac cctgaagtta cagtggagtt agacacatta atgttgttgt cccactgatg
     8761 atattcgcta gtataagtcg tgttgcttgt attgccttca gacagcgcaa taccagctac
     8821 acgggagttg ttagtaatat tgatattgct ttcaacatca agatttactg caacacccag
     8881 agcggtatag aacgtttcat tggttgactt agatttatca ccatcaagat atgtgtcagt
     8941 gaagtaaaga gcttcatagt catcatcaat ggtggaattg gatacgttca gagtgaatac
     9001 gtcaccatcg taccaattgt agtcaaggtc agtagtgatt ccgttataga aattatagcc
     9061 attataggtc tgtggagtta caacttcacc tttagcatta acttctgcag gagtaatgct
     9121 gtaataacca aatggcagtt cagaggtgat tgccccatga attactgaat tgctgatatc
     9181 tacctggtta acagaaccgc tagcgtaatc atgattcaag taataagtag aaatggtacc
     9241 gttaacggta tcgcctttaa agacggtatc gaaatcgcca ttggcattgt tatagtaaac
     9301 aaaaccattg taagttacgt agttaccgtc tgaacccatc gggtccatgc cgccagcatc
     9361 ggcaattcca tcattgtcag tgtcatacgg ccaaactaaa tcggtcgtgg tgttgtcatg
     9421 atacccccag gtatcatagt ttgctgcatt agcctgagag gtgatagcca gagtgcaagc
     9481 caatgctaat tgtgatacta caagtttctt tttccaggag tgcatttgtc atccctcctc
     9541 agggacgtaa taagttgatc catcaattat caatgcatag aaaacatatt atgtttcgcg
     9601 tttacgatta tgcggcgtta tagaggaatg gttcaattat tctttcccct aagtccgatt
     9661 gcggacatat ataaagcacg tgaacacaca caaagaaaga aataacaaaa acacaatatt
     9721 tcaatacgtt aatgaaagcg ttaattttaa aacatagacc atgaaaatat aaaacaacaa
     9781 aaacagacaa ataatgtatc aataagcaat gaactttaag ctattcactt tttgaaaacg
     9841 tcaccaaatc cccaaaaaca ataatctatg gctataaata aaaattaaaa atattttcag
     9901 tgaatatgaa
//
LOCUS       DAWDRC010000064         9812 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3495_length_9812_cov_2.335144, whole genome shotgun sequence.
ACCESSION   DAWDRC010000064 DAWDRC010000000
VERSION     DAWDRC010000064.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3495_length_9812_cov_2.335144"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1960..1969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2300..2309
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2406 a         2726 c         2476 g         2184 t
ORIGIN      
        1 cacctgcggt ccgttgccgt gaacaatcgc cagccgataa gaacgggcca ggcgcgccag
       61 cgcgggtaca gcactggcaa tattgcgata ttgatttttt gccgtcagcg cctcaccgcg
      121 ctggagtaag gcgttgcccc caagagccac aaccagtgtt ttcatggttt tccttgtaat
      181 aattgttggc caagccagaa ccccagcagc gtatcggccc ctgacgtatg ccccagcgcc
      241 agcagcgaat cgatcgccgc tgcggtacgt ttcgggcaac tcagagcatg aacaaagtgc
      301 aggagtggcg aggcgaaata tccttgcgcg gcataacgta aataactgac gctcaccgca
      361 gtggtaacga gttgaagatt gtcggaacag gcaaaaaacg ggcgaccgga gcgcgcatct
      421 aaagcaccat aataccaggc cgccagcagc attccgctca gcgtgtcatc atgacttggc
      481 gttaatccgg ggcctttacc cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg
      541 gcctgaaaac agtgacgaaa ctggcgtaat tcagcaggca gcggatcgct tgccgccaat
      601 gccagtggcc cgaaaagccc ggtttcctcc gcgcgttgca tccatgcaac ggcaagcgct
      661 tgaggatgcg caggcggcgt aatacgtagc aaacaataac gctgtggctg tttaaccgtg
      721 aaacgcccga ggcgaatccc ttgtgccaca acctgtggtc gttcattgcc gcataatcca
      781 ccgcataacg catcgaattg cgcgcggcga agcacccatc cgccggggcc gaaaccactc
      841 ccctgacgat gcagcgttaa cagttcgccg ctttccgtca tcaggttaat cgcccgccgc
      901 cacactcccg ctaaccgcca ggactggcga taattcggtg cgctacggct ggcaagtaga
      961 ggatggatga tcgtcattgt gcgttcctca accaatcccc atgctttcag ccagtgcttc
     1021 cagcgcctgt tcaaagcacg ccagcggtgc ccgcacggtg cctgcgccaa tctgcccgat
     1081 ccccgcctct ttatgggcga taccggtatt gatgagtggc gtaatgccgg tttctaccac
     1141 gcgacgaatg tccagcccca ggcacgcccc ctggaaatcc cagccgggga tctgcaattg
     1201 catattgcgt tcaaggtaaa tttccgccat ctcttcagat accgctctgg ccgcttccat
     1261 gccacccgca ccgacaaagc gcgttacgcc aggcgctgcg atcattgccg ctccgccaat
     1321 accaaaggtt tcggtaatcg cgctgtcgcc catatccggg ttcgcctgat cctgcgagaa
     1381 gccggtgaaa aacagacctt ggggagtgtt tacaggcgcc gtaaaccagc gttcacccag
     1441 cccgcttacc cgaatcccga acatattgcc gttgcgggtc atagccgtaa cgatgctgcc
     1501 tgcgcggatc atcgcgcctg catccatcgc cgccttgcag taggccatcg cgagattgag
     1561 gaagaactga tcggtcacgc tgaggaaatc catcacttcg gcgatgtgct gtttatcatg
     1621 atcgaggcga gcaatttgtg gggccagcgc acgcattaac agtgcagagg aagcaatatt
     1681 gcgttgatgg aactcatcgc ccatggtaat gccctgcgcc atcattgccg tgagatcgat
     1741 accgcgctcc atgcgcccca gcgccgcgct taatactggc attaacacat cgcgcatcca
     1801 gcgatggcga gtcaggacat cttcgccgta agcgccaaaa cgcatcactt tgccgatacc
     1861 ttcgttgagg ttgcagtacg cccggttgcc gtcggtcacg ttctcgacca ccagcatcgg
     1921 catactggca gaagtaatgc cacccattgg cccgacggcn nnnnnnnnnt atgcggatgc
     1981 tggtcgtcga gaacgtgacc gacggcattc acatggtgac aaggaatgaa gttcacttcc
     2041 ccctgctcca gtattgccag cgcctgcgct tcatctttcg cccagccttc gaacagacat
     2101 gcgcccacgc acgccccttt catgggtccg gtcatctcct gccagcgcat tggcggcccg
     2161 gcgtgaagca gtgttttgcc cttgtttagt tcgctgataa gtgaagaagc cggttgcaca
     2221 tccagccagt gtggacgagc gcgacgaatt tgttcgataa ccgcagcatt ggcttgcgcc
     2281 actgatgtaa acatgggaan nnnnnnnnnt gggaacccct tattgcaaac gttctaataa
     2341 acgagccagt tttttattgc cgccggcgac tggcgaccat tggtaatgca caaccggttt
     2401 gctggcgctt tgtagctcca gcgcaaagct gcgtaatccg atgttaatca cggcgacgtt
     2461 ttccagtaat gacggtgtgt gttgctgtgt ggcaggcgag agcggatgaa ttaatgccgc
     2521 cgccagcaag gtggcttccg gtagcgaact cactaccgcg atccccgcat cttccagcgt
     2581 ggcaatttgc tgcgagcgac attgcggatc acgttcagtg cctgtcaccg tggcaatggc
     2641 atacagtggt tgattgtctg aacgcgcggc acaggctttt tgccaggcgc tcaccagcga
     2701 ggcggcagga tcggcggtcg caccgaagcc aatcacgaca tcaagcagca acacgcgcac
     2761 ttgcggttta gcgccgagat cggcaattaa caggttgcgt aaggtcgggt cgatcatcgg
     2821 atggggacgc ccgacggtgt aaaaatcatc gcccaggtcg aggatctgat gtccgtcggc
     2881 gtccagcatc atgccatgtt gatgggtatc gtcggcttcc acgccaaggt gtccggcaag
     2941 taatcccgcc gcttcagcag ccagcgtacc gccggtatac aaaccgcaaa taaatccgct
     3001 gctgacaggc gctatcgcgt tacgtcgcgc agtgacgcgt gaaagcagac aagccagacg
     3061 agcggcctca tccagcgagg aggcaaacca gacattctcg tcgcgcgcca ccgtcggttt
     3121 gccagttgct ttcatggcat taacaatttt cagacgcaca gtttcggcag gtggttttga
     3181 aacaaatgcc agcacttcgc ttttctcgtc tgcactgagc atttccagcg ccgttagcgc
     3241 actgatgccg cccacttcac ggctgagatc gcgcccgcca agaccaatcg cgtgagtaat
     3301 tccctcccct gccagcgcaa tctgtgagca cagctcctga atcccggtac cggaagcgcc
     3361 tatgacgcca atgttgcctt ccggcatcac gttagcaaaa gccagcggcg tggcggcaat
     3421 catcgacgta ccgcagtctg gccccatcac cagcaagcct ttttcccgcg cgcgggtttt
     3481 aagttggatt tcatcttcca gcgtgacgtt atcggaaaac atcatcacgt tgaggttgcg
     3541 atccagcgcc tggttcgcca gctccgccgc atactcacca gccactgaaa tcagcgccag
     3601 attggcatcg ggtaatttct gacaggcact gtcccagcga cgcacctgcg tcaacgcctg
     3661 gctgctgcct gaaccctgcg ccagttgttt tagcgcctct tcaagctgct gcataaccgc
     3721 ctgcgcgatc cccgcatccg ccgcttcgct acgaattgcc acgcaaatat cgttcggcgt
     3781 ggcgttatta aaatcgtcat gccagaaacc tgtagtatct aataacgctt tattggcggg
     3841 cgtacccatc attacggaaa catcatcaac attttctgat tcgctgagtt ttcgtgaaat
     3901 aatcattaaa ctgaccgaat cctgaaaaca ccctttttta ataaaggcgt ggatcataac
     3961 aactccttaa aaattgcacc catggcaatt gcacgagcag tgttaatgtg gcgattacgg
     4021 tatcgatgac aacgagtaaa taaggtgatg aatatcacac gtggattatt aacaaaagaa
     4081 ttcaaataac cgccagacta ttacgcgtta ttttttaata agcatggttg ttttaaaaac
     4141 caggttaaaa gcacgaagat tatcaaataa attctaacta tatttttttg cctgtctgga
     4201 tcacataatc cagatatttt ccctgtatgt taattgcagt catgcttcac accgtcgtta
     4261 aaaaggaaga cagatgaaaa tcagtcggga aacactccac cagctaatcg agaataaact
     4321 ctgccaggct gggttaaaac gtgagcacgc tgcaaccgtg gctgaagtat tggtttacgc
     4381 cgatgccaga gggatccact ctcatggcgc ggtgcgcgtt gaatactacg ccgaacgcat
     4441 ttcaaaaggc ggcaccaacc gcgaaccgga gtttcgtctt gaggaaaccg gaccgtgctc
     4501 ggcaatttta catgccgaca atgccgccgg acaggtcgcg gcgaaaatgg gtatggaaca
     4561 tgccatcaaa accgcccagc aaaaaggcgt tgcggtggtc ggtatcagcc ggatgggtca
     4621 cagcggcgca atctcttatt ttgtgcagca ggctgcccgc gccggattga ttggcatttc
     4681 gctgtgccag tccgatccaa tggtggtgcc gtttggcggc gcggaaattt actacggcac
     4741 taacccactg gcctttgccg cgccgggaga aggcgatggc atccttacct tcgatatggc
     4801 gactaccgta caggcatggg gaaaagtgct cgacgcccgc tcgcgcaata tgtctatccc
     4861 ggatacctgg gcagtcgata aaaacggtgc accaacaacc gatccgttcg cggtacatgc
     4921 tctgctcccc gccgctgggc cgaaagggta tggcctgatg atgatgattg acgtcctctc
     4981 aggcgtcttg ctcggcttac cgttcgggcg acaggttagt tcgatgtatg acgatttaca
     5041 cgccgggcgt aatttggggc aattacatat agttattaac ccgaactttt tctcctccag
     5101 cgaattattc cgtcaacatc ttagccagac catgcgcgaa ttaaatacca ttacccccgc
     5161 gcccggtttt aatcaggttt attatcccgg acaggatcag gatattaaac aacgcaaagc
     5221 cgccgtcgaa ggcatcgaaa ttgttgatga tatttaccag tatttaattt ccgacgcgct
     5281 ttataacacg tcatacgaaa cgaaaaatcc ctttgcgcaa taattattga gacaggactt
     5341 tcttatgatt acacatttcc gtcaagctat agaagaaacg ctgccctggc tttcctcttt
     5401 tggcgctgac ccagcgggtg gaatgacccg tttactttat tcgccggaat ggctggaaac
     5461 ccagcagcag tttaaaaaaa gaatggcagc aagcgggctg gaaacacgtt tcgatgaagt
     5521 ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg ttctgagcgg
     5581 ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg gtgcgctggc
     5641 ggcgtggctg gctattgact ggctgaaaac gcaatacggc gcgccgttac gtacggtcga
     5701 agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgttttct ggggcagtaa
     5761 aaatattttt gggctggcga atcctgacga cgtgcggaat atctgtgatg ccaaaggaaa
     5821 tagttttgtc gatgcgatga aggcttgcgg atttactctg ccgaacgccc cactaactcc
     5881 gcgtcaggat attaaagcct ttgtcgaact gcatatcgaa cagggctgtg tgctggaaag
     5941 taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgtt atacggtgac
     6001 gctgaacggt gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc gtgatacggt
     6061 ttacgctttc agtcgcattt gccatcagtc tgtcgaaaaa gcgaaaaaga tgggcgaccc
     6121 gctggttctg acctttggca aagtagagcc gcgcccgaat acggtgaatg tagtgccggg
     6181 taaaaccacg ttcaccattg attgtcgtca taccgatgct accgtgctgc gcgatttcac
     6241 ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggatattg gtattgatat
     6301 cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg ccaccctgac
     6361 agaattgtgt gaaagcgaaa aactgaatta ccgggtgatg cacagtggtg ccgggcacga
     6421 cgcgcaaatt ttcgcgcctc gcgtgccgac ctgcatgatt ttcattccca gcatcaacgg
     6481 gatcagccat aacccggcgg aacgcaccaa tattaccgac cttgccgaag gggtcaaaac
     6541 gttggcactc atgctttatc aacttgcctg gcagaaataa ggagttataa atgggatatt
     6601 taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt gttaaacacg
     6661 gtaatttcgc actgttaaca ccagacggtc tggtaaaaaa tattattccg ggctttgaaa
     6721 attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt gattatctgg
     6781 tcacactgca tcaaaacggc ggcaaccaac agggcttcgg tggcgaaggc attgaaacgt
     6841 tcctgtatgt gctctctgga aatatcactg ccaaagccga agggaaaaca tttaccttaa
     6901 gtgaaggtgg ctatctttat tgcccgccag gctccttaat gacgtttgtt aacgcccagg
     6961 ccgaagacag ccaaatcttt ttatataagc gtcgctatat tccggtagaa ggtcatgcgc
     7021 cgtggctggt ttctggcaat gccagcgaac tggaacgcat tcattatgaa ggcatggacg
     7081 atgttattct gctggatttt ctgcccaaag agttaggttt tgatatgaac atgcatatcc
     7141 tctcttttgc accaggtgcc agccacggtt atatcgaaac ccacgttcag gaacacggtg
     7201 cctatattct ttccggtcag ggggtttata acctcgacaa taactggatc ccggtgaaaa
     7261 aaggcgatta catctttatg ggcgcttatt ctttacaggc tggttatggc gtagggcgtg
     7321 gtgaagcgtt cagctatatt tactcgaaag attgcaaccg cgacgtagag atttaagtca
     7381 taccactcgc ctgattattt ttagctatgt tggattttgc cacggtttat tccgtggctt
     7441 tttttgtagt caattcaggt aaaaaaagat gcgatataaa tcgcatccgc tttaaaggta
     7501 atagtcagtt tttaattccc tgacctattt taatggcgca ggcaatattt cgcgcactgt
     7561 tgaagagatt ggtttcaccg ctggcgagca cttcggctaa aggtgccaga cgtggcaaaa
     7621 tgctgaatac cgcatcaatg ccgtactggt gcaccacttc cacgccatca cccaatacgc
     7681 cagcaatccc aatcaccggg acattaaact gcttcgccac cgacgccacg cccagcggcg
     7741 ctttaccgcc tgccgtttgc gagtcgatgc gcccttcccc ggtgatcacc agtgctgcac
     7801 cctgcaccgc ctgcgccaga ttgaccgcat tcaacacaat ctcaataccc ggtttaatat
     7861 ccgcattgag gaataccgcc gccgcaatgc ccataccgcc cgcagcgcca ccacccgcca
     7921 tctggcagac attaatttca gtttgctgtt gtaaaacacg ggcgtaattt tgcagcccct
     7981 gttccagttc ttcgaccatt tccggcgttg cccctttttg cggaccaaac accgccgccg
     8041 cgccgcgtgc ccctaccagc gggttatcaa catcacatgc tacttcaata tggcagttcg
     8101 ccagacgcgg atcgcattca tccatctcaa tgcttgccac gcgcgctaaa ttgccgccgt
     8161 tagccgccag cacctgaccg tcagcatcaa ggaaacgcac accgagcgcc tgcgccatgc
     8221 ccataccgcc gtcgaccgtc gcactgccgc caatgccgag aataatatgg cgaatgccgt
     8281 tatccagcgc atgacgaatc agctctccgg tgccaaaact tgacgccagc aacggattac
     8341 gcttttcagg cgcgaccagc atcaggccgc ttgccgccgc catctcaatc accgccgttt
     8401 tcccgtcgcc ggtaaggcca taaaaagcat tcactgtttc gcccatcggc ccgcagactt
     8461 caagcgtcac gatgttgccg cccgtcgcgg cgaccatcgc ctccaccgtc ccttcgccgc
     8521 catccgctat cggcaaacag atatagtgcg catcgggaaa tatggtcgaa aacccggctt
     8581 taattgcctg acaacatttt tctgcactta aactctcttt aaacgagtct ggcgcaatga
     8641 caattttcat tatcaatcct ttttcaacaa attattcgat gcgttcccac ggaataagat
     8701 tatccataag aacagccagt aaaatcccga ccagtaagcc gttactgagc agaggacgca
     8761 gcgttaatgg aaggtcttgc agatacacag gcggtaatgc cattaaaaat atgccgacaa
     8821 ataacggcaa tgcgagtcga taaatattgc gagcggtaaa ggttatttgc tggctaaaca
     8881 ccagcgcgga aaagagtaaa ggcagataag aaaccagcat gactgcacta ctcacgggta
     8941 aagggatact gcaaaacagt cgcgtgagtg ccggaaccag tgccaccagc aggcaaataa
     9001 cgctgccata aataaacgaa cgccgcgtgt aatcgcctgt ctgggttaat aaaccaatgg
     9061 atgaaacaaa cggtgaaaat ggaattactg ccagcggcac ggttatcagt gtcataaatc
     9121 cggtcgccac aaagctgcga cgataacgcg tattccccgc gccctgctgc ggataaaaaa
     9181 catccgtgcc ccgaatcgca ccgtaggtat tgctgatatt taccagacct gtaatcaccg
     9241 ccgtcagaat aatccccggc gacaaagcac cgccactgcc gagcgggaac cactgccagt
     9301 gcaactcacc ggagagcgaa tgcgaagaag gaaagcaaaa gtaccacaac aaccagccgg
     9361 ttatcgtacc gaccagcagg ccgtaacggg caaaacgttg tggcaggaaa ataatcatcg
     9421 ccagtaccag gcacatcacc gccaccgaga gcgcgaacgg cggtaattga attttaaaat
     9481 tcgggtcggc tatgccaaac ggcagcccga gcataccttt gaaaaagata gtggtcagtt
     9541 gcgcacccag catcaacata aacaagacca tcaccgacgg cgtaaataac cgtgccaggc
     9601 gatggcctaa tccgctaaaa ccaatcaaca tcgtcagcac gccggagagc gcaatcccca
     9661 ccgccagact ggtagcgata tcgttgatcg gtgtcccgcg cgatgcttcg ccaagggtga
     9721 tagtcaggat ggttccccac cacaggccgc ccggcccttc cataatagcg cgacgatgac
     9781 cgcagaacgc ctgagcgaag caggccagtg cg
//
LOCUS       DAWDRC010000065         9784 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3505_length_9784_cov_2.684962, whole genome shotgun sequence.
ACCESSION   DAWDRC010000065 DAWDRC010000000
VERSION     DAWDRC010000065.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3505_length_9784_cov_2.684962"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1082..1091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8798..8807
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2473 a         2715 c         2249 g         2327 t
ORIGIN      
        1 accgagcact tcaacaatgc ccatcactgc cggttcttcc atttcgtacc aggcttcgca
       61 gccttcagcc tgcattcgtg gcccggcaac accaacaaag cgggcgttgg gcacacgttc
      121 tttcagagcg cggattaaac cggcccccag gatatcgccg gaggtttctc cggcgaccag
      181 ggcaatcgtt aatggacgct gttcagtcat taacgaatca gaccgcgcgt tgagcgtgca
      241 aagaaatcgg taaaggcttt cacttccgga tatgtttccg ccagttcagc aatttccggt
      301 ttcacttcat cgagcgtttt accgctacga taaatcagct tatacgcatt gcggatagcg
      361 gtaatcgcct cacggctgaa tccgcggcgc ttcagccctt cgatattgac accgaacggc
      421 gttgcgtggt taccctgcgc aatgacataa ggagggacgt cctgcgccac accggagcag
      481 ccgccaacca tcacatgcgc accaatgatg cagaactgat ggactgcggt catgccgccg
      541 atgatcgcga agtcgtcaac cgatacgtga cccgccagcg ttgcgttgtt ggcgaggatg
      601 cagcggttac ctaccgtaca atcgtgcgca atgtgcgcat tgatcatcag taagttgtcg
      661 ctgcccacct tcgtcaatcc accgccctgg actgtgccac gatgaatggt gacgctttcg
      721 cgaatgcggt tacgatcgcc gatttccacg cgggtcggtt cgccagcata tttcagatcc
      781 tggttaactt cgccgatgga ggcgaactga taaatctcat tatcgcggcc aattttagta
      841 tgaccattca cgacaacgtg agatttcagt acggtaccct caccaatttc gacatggggt
      901 ccaacgatac aaaaaggacc aatgtgagcg ttcgcgccaa tcgacgcgcc ctcttccaca
      961 atggcggttg gatgcacaaa ggcggattta tcaatcacgt atcaggcctc ccggctacga
     1021 gcacacatca tcgttgcttc gcaaactact ttaccgtcga ccagagcaac ccctttaaaa
     1081 cnnnnnnnnn naatggctca ttgacagaga cattttttac tgcgcgcaga aaacgacctt
     1141 cttcaaaatc cagcacgcga tccaccagta agaacgggaa acggtgcggc agaagttcta
     1201 aaatctcttc aatctgcaga gtatgagtgt tagtagtcaa gatactcttc ctgtcaaaat
     1261 ataagaaacg acaataataa cacggcctgc cgcaatcgta agaatgagac aggccgtaaa
     1321 gtttggcgaa caaaagatgg aacgttagtc ttgttgatta accttgcgct caagcgattt
     1381 cagacgcttg ctcatgtcat caatgttcat caccagtgca gcggttttgc gccagacttt
     1441 gttgggttgc agcggaatgc ctgaggaata gacgcctggt tcagtgatgg gacgcatcac
     1501 catacccatg cccgtaaccg tcactttgtc gcatatttcc atatgcccgt tgattacgct
     1561 ggctccgccg atcatgcagt aacgaccaat tttcaggctg cccgccataa tgacgccacc
     1621 ggcaactgcc gtattgtcgc caatcacgac gttatgtgca atctggcact ggttatcaat
     1681 gatcacacca ttgccaataa cagtgtcatc cagcgcgcca cgatcaatag tagtgcaggc
     1741 accgatctcc acgcgatcgc caataattac ccgaccaatc tgtgggatct tcacccagtt
     1801 accacgatcg ttggcataac caaagccgtc tgcgcctacc actgttccgg actggatcag
     1861 gcaattctga ccgatctgga tctcatggta aatggttacg ttcgcccaga gacgcgaacc
     1921 tgcaccgatt ttgctgtttt tacctacgaa gcaaccggca ccgataatca cgttatcgcc
     1981 cagttcaacg ccggactcaa tcaccgcgtt agcgccaatc gatacgttgt tacccagctt
     2041 cgccgtcgcg tcaatcaccg cactgggtgc aatgttctgc gcgggctgcg gcgtggtatc
     2101 taaaatttgc gccatgcgcg cgtaagtcag gtagggattc ttcactacca gcgcggcact
     2161 tttcgcgaaa ggaagatcgt cctgggtcat gacaaccgcg gacgcctggc acaagcctaa
     2221 atgctcacgg tatttggggt taaccatgaa cgtaatgtga cctgtttgtg cagattgcat
     2281 ggacgcaacg ccggtgatga cgatatcgcc atcaccgtgt agttctgcat ccaactgctg
     2341 cgctaaatca gccagtcgaa ttgaaggcat tacttattta acctgtttca gtacgtcggc
     2401 agtgatgtct tttacatcgc tgctgttgta agcaacggcg tttgcatcaa caaccagatc
     2461 gatatcctgg ctgttggcaa cggatttcac agcagtctgg atacgagtaa ccagtttgcc
     2521 gcgttcttcg ttggaacgac gtgcgcgatc ctgctcaaaa gcctgcgctt tctgagcaaa
     2581 agtctggcgc tgagccatca cgtctttttc cagcttagtg cgatcgctgc ccgctttcat
     2641 ggactgcagc tttttcattt tagcctgcag atcggtttcc atacgctgca gttcgctggc
     2701 acggcctttg aactcgtttt ccagcgtgtt agaaacaccg gttttctgcg ctacctgctg
     2761 gaacaggctg cccatattga cgattgcaat tttgtcagcc gcctgagcag aagttgccag
     2821 tgctaaaccg agacctgcag ctaataacca ctttttcaca ataaactcct taccatccca
     2881 tttgcaccgg agggtgcagt tctttgcgtg gcccggcgat cttatattga tcgcctaaag
     2941 tcatcgctac actaccacta cattcctttg tggagaacac ttaccaggtt ttaccgatgt
     3001 taaactggaa ctgttctgcc ttgtctccat cgtacttttt gaacggctgg gcgtaggaga
     3061 acaccaacgg ccccaatggg gacatccatt gtaatgcgat acccgcagac atacggatat
     3121 tgcttggatc actatagtcc ggatatccag aatattggct ggaatcccag tttgtatccc
     3181 aaacggtacc catatcccag aagaaggaag tacgaaccga gttagcatac ttatcgctaa
     3241 taaacggcgt cggggtgatg aactcgaggc tggcaaccgc catggcgtta ccgcctacag
     3301 catcatccga tttacacaga tctttcgcgc cgtcctgagt cgcacattcg taatcatagt
     3361 ccggatcata attactggcc tgatgcggga agtaaactgc tttcggacca atggtattgg
     3421 actggaagcc acgcacggtg ctggaaccac cggcatagaa gttctcatag aacggcatct
     3481 ctttgccgcc taaaccatca ccataacccc agcgggtacg acccagaaca acccatttgt
     3541 gatcatcatc aatcggcaca taagtcgccg tgtctaacgt cactttgtag tattcgttat
     3601 ccgatcccgg aatggtcact ttaccggtca ggttgacacg tgaaccatct gtcgggaagt
     3661 aaccacggtc aagcttgtta taggtccaac catagttgaa cgtgaagtcg tccgttttga
     3721 agctgttatc ctgatcagag gtgctcggat gttcacccat agagtacaga taacgccaca
     3781 tcgcaacctg cggctgcatg ttggacaggg agttatgtac ataacccaga cctgcacgca
     3841 gcgagttata ttcgttgatc gggaagccca acgtcacgtc tgtaccataa ctcttgttgg
     3901 tatagtcgga caggtcggcg tcatctgcct ggaagtcatt atagaagaga cgaccaccga
     3961 ggcttacgcc atctacggtg aagtacgggt tggttaccga cagttcagca taggtctggt
     4021 aatcgttttt ggtcccgttg ataccaacag cataacccgt acctaaccag ttatcctgtt
     4081 gcacaccagc ctggaagctc acgccacttt cagtaccgta accaatcccg aagttgaagc
     4141 taccggtgtt acgttctttc accttgtaaa ctacatcaac ctgatccggg ctccccggaa
     4201 cacgttgggt atcggtatcg acagtttcaa agaagcccag acggttcaga cgctccttac
     4261 cctgatcgac cagatcgctc cccagccatg caccttccat ctgacgcatt tcgcgacgca
     4321 ggacggcatc tttcgaggta tcgttacctt caaaacggat cttacgcaca tagaaacggt
     4381 tacccgcatc aacgttcacg cgtaatttaa cggttttgtc ggcatcgtta atttcaggca
     4441 tcgactgtac gcgcggatag gcataaccat agcgaccaag aagcttttta atgtcatctt
     4501 ccattttggt cactttggtg ccgttataca gctcgcccgg ctcgatctta gtcagttgct
     4561 caatttcagc ggagtgcccg gcaagattgc cgctaacttc aacgccagaa agcttgtact
     4621 gatcgccttc ggtgatgttc accgtgacgt aaataccttt tttatctggc gtcagactga
     4681 cctgggtaga atcgatgttg aaacgggcat aaccgcgatc cagatagtaa ctgcgcaggg
     4741 tttcaaggtc gcccgcaagt ttctgtttct ggtatttacg atcgcctacc acgttccacc
     4801 acggcacttc gtcacgcagt tggaaatgag agatcagttc atcggtggtg aaagcatggt
     4861 taccaacaat gttaatttgc tggatttcag ctgacacacc ttcctggaac accagtttta
     4921 ggtcaacacg gttgcgcggc agcggggtca cgacagcttt aacgctggcg ctatatttac
     4981 cgacgctgta gtagaagtct tccagacctt tctcgatatc ggcaatggtg gtgcgatcga
     5041 gggattcgcc cacacgcacg ccagaagcct cgaggttttg cttcagcatg tcatctttca
     5101 ccgatttgtt accggagaaa gtaatgctgg caatggtcgg acgttctttt acctgaacca
     5161 gaagggtatc accatcacga aggacgcgaa catcctcaaa gttgccggta gcaaacagag
     5221 cgcgaatggt attactgata tcttcatcat taaccgtgtc gcctgtgcgc accggcatac
     5281 tgaggagggc cgcaccaacg gcgacacgct gaaggccttc gaaatgaata tctttcacta
     5341 cgaacccttc agcaccgtat acggtggcgc tgctaaacag cagcgacgct atgagcaact
     5401 ttttcatcgc catcgttatt atgcgttctt cctaactaac tctcataacc gagagaaatc
     5461 attgaaaagt gcaagcccca ttaacagcac cagcagaatc gagccaatgc gataacaaaa
     5521 gtcttgaacc cgctcggata ccggtccgcc cttgatcttt tcgatcgcaa ggaacagcag
     5581 atgccccccg tcaagtacgg gcaacggaaa caggttaatt atccctaagt tcacgctaat
     5641 aagcgcaaga aacggcaggt aataaactac cccgagttcc gctgtcatcc cagccccctt
     5701 ggcgatagag atcggcccac tgaggttgtt cagtttcaca tcaccggtga tcaattttcc
     5761 cagcatactg accgtcagct tcatcaactg ccacgttttg tccgtggctt cgacgatagc
     5821 gttgaacggc ccatactggc gtacaacttt atactcatct ggcaaaggaa tgactttcgg
     5881 ctcaataccg acaaaaccaa tcgctttacc cttacccggt ttactctccg ggattaatgt
     5941 caaagacaag ggactcccct gcctttcgat ttctaacgct aaggatttac ccgggttatc
     6001 ccggacaagc atcacaaagg tcacccactg cgttaagggc tgaccatcga ctttaacgat
     6061 cctgtcgcct gcttgcaaac ctgccttgct tgccgccgag tttggctgca cattttccag
     6121 tacaggttca atttgcggcc cacgaggacg aatccccaac gaagataccg gatcttcttt
     6181 atcaggctca aacgcccagt gacgtaaatc gagctttaca tcccgccgtt ggtcgctgcc
     6241 aaatggcgct accgtaatgg tggtgctttc atcgccaatt ttatcgacca actgcaaacg
     6301 cacggcatcc caatcaggcg tttcgatacc atctacggct tttagttccg tacctggtgc
     6361 aatttgtgct tccgcagcta tcgaattggc tgctatttca ccaaccaccg gacgtacgcc
     6421 aggcacacca ataataaaaa ccagccagta ggcaaagata gcaaaaatga agtttgcaac
     6481 tggacctgcg gcaataatcg ccgctcgttg gccgacagat ttattattga aggcatggtg
     6541 gcggagttcc ggaacgaccg gttctgcgcg ctcatccagc attttgacat aaccgcccaa
     6601 cgggatcagg gcgataacat attcggtgcc gagcttatca gttcgccgcc agagcgcctt
     6661 accaaaccct attgagaaac gctcaacgcg aacaccacaa cgccgggcaa cccagaaatg
     6721 accaaattca tgcacggtga taagtacacc cagtgcaacg atgaacgaag ccaaatccca
     6781 gagaaaactc agcatattac cttccgttaa agcgtcctga ataccagtaa caacaagcaa
     6841 gcaaagaccg gtaccgcagc cgtcaggcta tcaatacgat ctaaaatacc accgtgtcct
     6901 ggaattaaat gaccgctgtc cttaattcct gcttcacgct taaacatact ttcggtcaga
     6961 tcgccgagca ctgaggctaa cgctgcgaca atagagcaaa tgagtaaggt gacgggagcg
     7021 acgtcgagat tcacccacat gccataaccc catgagatta ccgctgcggt cgcgagtcca
     7081 ccgataaagc cttgccaggt tttacccgga gaaaccttcg gtgccagttt atgtttacca
     7141 aacaatttgc caaacatata tgcgccagag tcagcgcccc ataccaggat catgacatag
     7201 agcagccata ttgcgccact gtaatgattc tcgtcgtagt gccaggcccg taacgccagc
     7261 atgccccaga agaagggaac aatggttagc acgccaaaaa taatgcgcaa tgttttagag
     7321 ttacgccaga ttgctgcgga acctgggtaa aacagcacca gcaatagcgc gacaatccac
     7381 caacccagcg aagcccaaag tgagatttca accagcggtt gatgaatatt tcggtgatat
     7441 tccggcaaca gaaaaagcat cagcgccaac aataacccgc ataacaccgc caaccatact
     7501 cgctgcgaac gagtggtaaa accgctaagc tgtccccatt cccacgctgc cagcatacag
     7561 accaccagcg ttacaatggc gaaccccacc ggcggcaaca gaaacaacgc cgcgatgacg
     7621 acgggtatta acacaaaagc agatatcagg cgatacttca gcaaaagcga cccccatcag
     7681 gctgtttcat caccgggctc ggtgccgccg aaacgacgct ctcgattagc aaaggcattt
     7741 aacgcccctt caaagtcttg ttcatcgaaa tcgggccaga gaacatctgt aaagtaaagt
     7801 tcggcatagg caatttgcca aagcaaaaag ttactaatgc gatgctcccc cccagtccta
     7861 attactaaat ctacaggggc cagttcatgc atacagacat gctggtttag catctcttca
     7921 tctatctgat ctggttgcag gtttccttgc tgcacctttt cagccagttg cctgactccc
     7981 tggactatat cccaacgtcc accgtagttc gccgcaatat tcagcgtcag accggtattc
     8041 ccggctgtta gcgcttcaga tttacgaata cgttcttgca aacgcgagtt aaagcgactg
     8101 gtatcgccaa taatacgcag acgcacgtta tgtcggtgca gactttttac ttcgctatct
     8161 agcgcccaca caaacagttc cattaacgca ctgacttcct gcgctggtcg gttccagttt
     8221 tcactactaa aggcatacag cgttaacgcc tcaataccgt tgttggccgc aaaagagaca
     8281 gcccggcgga cggattttgc cccggcttta tgcccaaagg cacgaatctt cccttgcttt
     8341 tttgcccagc gaccattgcc gtccataatg atcgcaacat gacgacagcc atgcgctggc
     8401 aatttttcgc taagtggttg agtagcagac aacatcacgc gtttcttatt ccctgacagg
     8461 gtttacggta ctcaggaata ctgaagccta tacaaaaaaa agccgtgtaa aaccacggca
     8521 tacctgacct tataaagcca actacttacg atcagatggc gcagactata tcactgaagc
     8581 cctacgctaa caaatagcgc gactctctgt agccggatta tcctcagctt gcgagacgca
     8641 tcacctcttt tctggcgact tcacgcgcgt tcgcatcaac agataacaca tcatccacac
     8701 attgtggttc gcgcatatcc attttttcca gtacggataa attcaacgca gcgatatccg
     8761 taaagcggat ttgttgcgca agaaaagcag ccacggtnnn nnnnnnnggt gatttcattt
     8821 gcggcattca atgctgtcgt cgctgcctgg ccttgttcaa acgcctccat cgccagtttc
     8881 agacatggat aacgatcata atccggtgcg gcaaatgtca acgcacttag tttgcaaaaa
     8941 tcgagcggct tcacgccaga gttcacgcga ttcggccatg ccatggtgtg agcaattggc
     9001 gtacgcatat ccggttcacc cagctgcgcc agaacactgc cgtcctggta gcgcaccatt
     9061 gagtgaatca ctgactgcgg gtgaatcagc acttccatct ggctggcgct ggcgttaaac
     9121 agccaacgcg cttcaatgta ttccagacct ttgttcatca tggtagccga atcgacggaa
     9181 attttacgtc ccatcgacca gttcggatga cggcaggctt gatcgggcgt cattgttgcc
     9241 aaatcgcgca atggcgtctc acggaaaggg ccaccagacc cggtaagtaa aatggacacc
     9301 acgccatttt gctcaaggtc agcgtatccc agattatgct ggataggttg cggtaaactc
     9361 tgaaaaatag cgttatgttc gctatcgacc ggtaacaatt gcgctttgct ctgctttacg
     9421 gcgtccataa acagacgtcc gcaggtaacc agtgattctt tattggccag caaaatggtt
     9481 ttacccgcgc ggatcgcagc aagcgtaggt aacagcccag cagcgccaac aatggctgcc
     9541 atcacctgat caacatcctc aagcgcagcc atatcgcaag cggcttgttg cccacttaag
     9601 acttcggtgc ggctcccctg ttgctgtagc atcattttaa gaagtttcgc actcgcttca
     9661 tcgtccatta cggcatagcg gggagagaat tccaggcact gttctaccat gcgagtgaca
     9721 tttttgcctg ccaccagcgc aactacgcgg aagtgttcgg gattatggcg caccacgtcc
     9781 agcg
//
LOCUS       DAWDRC010000066         9745 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3523_length_9745_cov_2.429515, whole genome shotgun sequence.
ACCESSION   DAWDRC010000066 DAWDRC010000000
VERSION     DAWDRC010000066.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_9745_cov_2.429515"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5758..5767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6559..6568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9329..9338
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2457 a         2492 c         2523 g         2243 t
ORIGIN      
        1 catataggtc cagtgcgtcc cggcatcagg cgcgggcagg ttacgtaact gccactgctt
       61 aatgttgtac tcatcggtgc ggtacatctc tgggacggtg tcacctggtt tgaaatgctt
      121 aaagtcagca atgaaactgc ttaactcata cttagaaatt cccgaggtat tggtcgaacc
      181 cgcggtggct ggtgcggccc aggcggcact ggtaaccagc aaaagcgcac ccagcaacat
      241 cttattttta atggtcataa actctccact ttggggatct gtaatatcag tcgcatatct
      301 ttcctgccat acaatataac tgacaagcga tattttgtta gtgatcccat gatttcgtaa
      361 aaagtgtaaa tgaaaggatg ccctaaaagc atccttagcg agcagagagt cagcggaaat
      421 agctgattta agaatcatcc tgggctgaag ataacatgca agcctaaagg attgagttat
      481 cgcattatgc ccatgaagcc atgtacgccc atatacacac caactaagcc aatcaacaga
      541 ctggaaaaat agggagcgcg tttagcgaga gtgttaaatc cgctccagcg ttttgcgacc
      601 tgctgaacgc tgattgctgc gccaacgcct acggtgacaa gcgttaacgc caggccgagg
      661 ctgaaactga cgaccagtgt tgcgcccagt gtcagggctt tcaactgaat gcaaatcaac
      721 agcacggtaa ttgctgccgg gcaggggata aggccaccgg ttaagccaaa tagcaaaatt
      781 tgccagttgg tgacctctct accatcaaag cgtcgtttaa tgtcattggc atgggctcgt
      841 gcatgggcat cctgatactc gccatgttcg tgatggtgat gatgtccatg gtcgtggtga
      901 tcttcgtgat cgtggtgatg atgctcatga tcatgttcgt gcatattctc cagccagttg
      961 cgttcgccgc gccaggtacg ccagaacatc cagaacgcgg tgctaataat gatcactgcg
     1021 gaaatcaact ggagccacgg ttctgctgat tgagcagtaa agcgtttgct gatcaccatc
     1081 ccgccaaagg caattaacca gaccactgcg gtatgtgaaa tagttgctgc cagtccgagc
     1141 atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat catcgttttt
     1201 gagtgccccg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg gatgaagaac
     1261 caggcgtttc cttgctgaag aagtgttgta aattcggtca tgataataat tcttagtatt
     1321 aattcggcaa tctgattcta ctccccccca gtacctgata ctacccccca gtagattaat
     1381 agtgctatga tttttcatat tcttgttaac caggtgttgc catgtctcat acaatccgtg
     1441 ataaacagaa actgaaagcg cgtgccagta agattcaggg ccaggtcgtg gcgctcaaga
     1501 aaatgctcga cgagccgcac gaatgcgctg cagttttaca acagattgct gctatccgtg
     1561 gcgcggtgaa cggtctgatg cgggaagtga ttaaaggtca tctgacggaa cacatcgttc
     1621 accaggggga tgagctaaaa cgtgaagaag atctggatgt cgttctgaag gtgctggatt
     1681 catatatcaa ataatttatt aacgcgattg taaaactgcc gtttttcctc gtttacaacg
     1741 cgtgcgctgg acattaccat cctcctttgc gatttatcat cgcaaccaaa cgactcgggg
     1801 tgcccttctg cgtgaaggct gagaaatacc cgtatcacct gatctggata atgccagcgt
     1861 agggaagtca cggaccacca ggtcattgct tcttcacgtt atggcaggag caaactatgc
     1921 aagtcgacct gctgagttca gcgcaatctg cgcacgcgtt acaccttttt caccaacatt
     1981 cccctcttgt gcactgtatg accaatgatg tggtgcaaac ctttactgcc aataccttac
     2041 tggcgctcgg tgcatcgcca gcgatggtca tcgaaaccga agaggccagt cagtttgcgg
     2101 caatcgccag tgcgttgctg attaacgttg gcacgctgac gcagtcacgc gcacaggcga
     2161 tgcgtgccgc cgttgaacag gcaaaacgct ctcaaacacc ctggacgctt gatccagtag
     2221 cggtgggtgc actcgattat cgccgccgtt tttgtctgga acttctgtct cataagccaa
     2281 ccgccatacg tggtaatgct tcggaaatca tggcattagc tggcgttgct aatggtggac
     2341 ggggagtgga taccactgac gccgcagcta atgcgatacc tgctgcacaa acactggcac
     2401 gggaaactgg cgcaatcgtt gtggtcactg gcgaggtgga ttatgttacc gatggacatc
     2461 gcattgttgg tattcacggt ggcaatccat taatgaccaa agtggtagga actggctgtg
     2521 cactatcggc ggttgtcgct gcctgttgtg cgctaccagg cgatacgctg gaaaatatcg
     2581 catctgcctg tcactggatg aaacaagccg gagaacgcgc agtcgccaga agcgaggggc
     2641 caggtagttt tgttccacat ttccttgatg cgctctggca attggcgcag gaggtgcagg
     2701 catgaaacga attaacgctc tgacgattgc cggtactgat ccgagtggtg gtgcggggat
     2761 tcaggccgat cttaaaacct tctcggcact tggcgcttat ggttgctcag ttattactgc
     2821 actggtggcg caaaataccc gtggcgtaca gtcggtgtat cgcattgagc ctgattttgt
     2881 cgccgcccag ctcgattcgg tgttcagcga tgtgcgaatc gacaccacta aaatcggtat
     2941 gttggcggaa accgatattg ttgaagcggt ggcagaacgg ttgcaacgtt atcagatcca
     3001 aaacgtggta ctcgacaccg ttatgctggc aaaaagcggc gacccgctgc tttcaccttc
     3061 ggcagttgcc acgctatgca gtcgattatt gccacaggtt tcattaataa cgccaaactt
     3121 gcccgaagca gccgccttgc tcgacgcgcc acacgcgcgc accgaacagg aaatgctgga
     3181 acaagggcga tcgctgttgg cgatgggctg tggcgcagtg ctaatgaaag gtggtcatct
     3241 ggatgatgag caaagcccgg actggctgtt tacccgcgag ggtgaacaac ggtttaccgc
     3301 acagcgcatt atgaccaaaa acactcacgg cactggttgt acgctctctg cggcgttggc
     3361 tgcactacgc ccgcgccata caaactggac tgacaccgta caggaggcaa aaagctggct
     3421 ttcatcggcg ttagcccagg ccgacacgct ggaagttggt cacggtattg gtccggttca
     3481 ccacttccac gcctggtggt gagcaccgta ctatagctgc tttggttatt gtcagccttt
     3541 ggtgctgaca ttcagggatg aaaccaatgc agttgagaat caccagtcga aaaaaattca
     3601 ccgttttatt atgcgctctt gggctaattt ccatcgttgc aatttatccg cgtcagacgg
     3661 tgaatttttt ctattcgaca gcaattcaga ttaaagacta cattcacttc tacggttatc
     3721 gctcggttaa atctttcgct attcgcattc ctgccagtta caccattcac ggaatagatg
     3781 tttcgcgctg gcaggagcgg atcgactggc agcgggtggc aaaaatgcgc gataacggta
     3841 ttcgcttaca gtttgctttt attaaggcga cggaaggcga aaagctggtg gacccctatt
     3901 ttttgcgtaa ctggcaacta agccgcgaaa atggcctgct gcgcggggcg tatcattatt
     3961 tttccccgtc ggtatctgct tcagttcagg cgagattatt tctgcaaacg gtggatttct
     4021 cacaaggcga tttccctgcg gtactggacg tagaagagcg aggaaaatta tcggcaaaag
     4081 aattacgcaa gcgggtaagt cagtggctaa aaatggtcga aaaacgtacg ggaaaaaagc
     4141 cgattattta ctcaggagcc gttttttatc acaccaatct ggcgggctat ttcaatgaat
     4201 atccgtggtg ggtggctcac tattatcaac gtcgcccgga caatgacggc atggcctggc
     4261 gcttctggca gcattccgac cgtggacagg tcgatggcat taatggtccg gtggatttta
     4321 atgtgtttaa tggcacggtg gaggagttgc aggcattcgt tgatgggatt aaataaacgc
     4381 cttaaataag aagcagtgat atactcccac cagattcccc ggcaggaaaa agacaaatgg
     4441 aacaagcgca tacccagctg attgcccaac tgaacgaacg tattttagcg gcggataaca
     4501 cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc ggggtgctgg
     4561 agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc gtttcgcgca
     4621 ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg cgttcgcgtg
     4681 gttacggcac gcagatcaac aacatcttcg aatactcgct gaaagaagcg cgaggttttt
     4741 ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaac aagcgtgtcg
     4801 tgaaatgtcc cgaagaagtc gcgcagcagc tggcggtcga agcgggaagt gatgtctttt
     4861 tgcttaagcg tattcgctat gtcgatgaag aggcggtatc gattgaggaa tcgtgggttc
     4921 cggcacattt aattcatgat gttgatgcca tcgggatttc tctttatgac tacttccgca
     4981 gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg ccggatgccg
     5041 agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc aagcaagtgg
     5101 cgcttgacca acagcaacgg cctattgagt acagcatcag ccactgtcgc agcgatctat
     5161 acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc gccccgatgc
     5221 ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag tggcagacca
     5281 gaggccagcc cggcaagtac gccaccggca tgactgtcgc ccgccccaat ggtgtctaca
     5341 acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata ccatgcgccc
     5401 tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg cttgccaagt
     5461 gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc ttgacgattg
     5521 agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcatccgg gatatcgcca
     5581 atacgtgggc caaaatcgat aaacggcgtc acgtcttgca gcttttccag ccattccact
     5641 aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag gctgccaggc
     5701 gcaacggtta atcgcgccag ccactggcga ttccactgat tttccacacc gctgaatnnn
     5761 nnnnnnncat caggctcaac cagcgccaga caccagccgt tatcaccttc ggcgttatcg
     5821 atcaggctga ttaagtcctc ttttgccatc cggttgcgaa taatctccgc ccacaccccc
     5881 tgaccgagcg gcaaggcatt acccgcttcg atgccgaggc gctttaatgc cacggcaata
     5941 ttcagggcgc agccgccaac gttaacgctc tgctgtttca gttcgatatc gcacccacgc
     6001 caggggaggg cataagcgtc ggcgatcaca tcaatgaccg ccgcgccaat caccataact
     6061 ggctgacgcg aggttaattc aggcagcagc gtgtgtaatc gagcgccgct catatcgcct
     6121 cccgttgttg gcgatacttc gccagcgccg tggcatagcg gttgaaatca agctgattta
     6181 ctgtatccag ttccgccttt aacgcaggat cgatagtatt aacgccatgc aacgcgccgc
     6241 aaattgccgt cgccatagca ccgatggtgt ctgtgtcgcc gccaaggtta gcgcacagga
     6301 cggcgcaacg gttcgggtcg gtttgtgcca gttcaaccag cgcaatggcg cacggaacgg
     6361 actcaatagt actggtacct gcgccaacga cctggtaaag ctgttcgctg gcggattcgg
     6421 tgccgtcagc attgcgcaca attttcagcg caatctccag acgtgcagcc agtgaggcgc
     6481 tgaaggtagt gatgcgtttt tgttgagcat gtcgcgcaat tgaaggcagt gaatcaacaa
     6541 ttgctgacca gctttctcnn nnnnnnnnga aggagaaatc gcccatgcga tgactaccgc
     6601 ccccgcaacc gccagatcgg atttatgtgt cgggctggag gctagcgcca catcatcaat
     6661 aaaggaatca acatcacgcg ccggaagcaa acaacctaat ggcgagatgc gcatcgctgc
     6721 gccgttggtc actccgttat tttccagttc agcaacgggt ttaccgtcgc gaatggcgtt
     6781 aagcgcaatc ttcgaggtcg gacctagtac gtttttgtta aaggcgtcga aacgcagcgc
     6841 ccagtcgaga atattacgcc caatcagatc cggatcgatc ttgccttcac gttccagtaa
     6901 cgcatccgcc agacacagcg ccatcgaggt atcgtcggtg aattcggcgc ggttaaaata
     6961 acaggctgcg ttattctcct ttggcccagg cagaaaacgg tcaatccagc caaagtgtgc
     7021 tttgacgcga ctgcgcggcc aaagctcgga gggcatcccc atcgcatccc ctaacgcctg
     7081 cccataaaga gcaccgagaa tacgttctgt tttcatttaa cttccccttg tgtcaacgca
     7141 atatcgcgat catcgacctt gatagccgta atttcgttgt cggattcgcg gaaaaaaatc
     7201 atgaacagca cggcgataat ggcaatcatc accgcgccga aagtccacat cccggcccag
     7261 ttgaaagtca gtccgtttac cggttcctga taagcgaaca tcttttccat catcacaccg
     7321 ccaagacgat agccgagcaa actgccgaag ccctggcagc agagcgtgat cagcccctgc
     7381 gcggcggtac gcatatgcac gggagctttt ttatcgacat agatgtaagc ggtaacgtag
     7441 taaaaatcgt aacttacgcc gtgcagcaaa ataccgagga acagtaacgc gtaggtgaaa
     7501 tattcatccg cactaccgta aataaagaag ccatagcgga tcgcagcggt gaccagacca
     7561 agcaacaata cctttttgat accaaagcgt ttagtgaaaa acggcaatgc cagcataaag
     7621 aagatttcag agaactggcc gagcgtcatc cagccagtgg cgtttttcat gccaacttcg
     7681 gtcaggtaac cgttggcaaa gatgtaatag aacgccagtg gcatcgcaaa caggaatgaa
     7741 cagaagaaaa agacgaggaa gttcttatcg cgcagcagga tcagcgcatc caggccgagc
     7801 atgactttaa tatccatttt gccggtgctt tttggtggcg tgtcgggcag gaaaaatgca
     7861 aacacaccga gcagagcaga acttccggcg gtaatcagca gcgggatgtt cgtcggtgag
     7921 atatcggcat accccagcat ttgcggcaag aatccacatg ccagaccaga ggcaatccag
     7981 ccgatagtgc ccatcacacg aatgcgcggg aaatcacgct ccacatccgg cacgttggca
     8041 aaagcgatac tgttagtcag cgcaatggtc ggcatatagg ttagcgagta ggccagcagt
     8101 aacgggaaga acccggcaaa agtggtctgt tgcgcagcga aatacatcag caccgcacca
     8161 gcgaacatca ataccgccag cactttttgc gccgagaaaa agcggtcagt gatggagcca
     8221 accagaatcg gcgacaggat cgccgcaatg gcggtacagg catacgacca gccaatttct
     8281 ccggcactaa aaccgctttt acttaaccac aaccacaatg gcacaaacca cgcgccccag
     8341 ataaaccatt caacaaacat catgaacgac agctttgctg ttgttttcat cttttaattc
     8401 cttcatgaca ggtaatgtaa ggtacgcgat gacaatacca cttaataata ccttttaaat
     8461 acctttgtgg tgaaaacttg accgcggtaa catttttcct gatgaatcga gccaacagaa
     8521 aacgctgaaa aaacatccaa aagatggaaa aactcgcccg taacgaccag gattatgatt
     8581 gcccgcaaaa ggcgggccag gacaatccat agccgatatc caatcggaat ttacgggagc
     8641 atagtaatga cagatattgc gcagttgctt ggcaaagacg ccgacaacct tttacagcac
     8701 cgttgtatga ctattccttc tgaccagctt tatctccccg gacatgacta cgtagaccgc
     8761 gtgatgattg acaataatcg cccgccagcg gtgttacgta atatgcagac gttgtacaac
     8821 accgggcgtc tggctggcac aggatatctt tctattctgc cggttgacca gggcgttgag
     8881 cactctgccg gagcttcatt tgccgctaac ccgctctact ttgacccgaa aaacattgtt
     8941 gaactggcga tcgaagcggg ctgtaactgt gtggcgtcaa cttacggcgt gctggcatcg
     9001 gtatcgcggc gctacgcgca tcgcatccca tttctcgtca aacttaatca caacgagacg
     9061 ctaagttacc cgaataccta cgatcaaacg ctgtatgcca gcgtggagca ggcgttcaat
     9121 atgggcgcgg tggccgttgg tgcgactatc tattttggct cggaagagtc acgtcgccag
     9181 attgaagaaa tttctgcggc ttttgaacgt gcgcacgagc tgggcatggt gacagtgctg
     9241 tgggcctatt tgcgtaactc cgcctttaag aaagatggcg ttgattacca tgtttccgcc
     9301 gacctgaccg gtcaggccaa ccatctggnn nnnnnnnntt aattacggtt ataccgacga
     9361 tcgagtctac agcaaactga ccagtgaaaa cccgattgac ctggtgcgtt atcagttagc
     9421 taactgctat atgggccggg ccgggttgat aaactccggc ggtgctgcag gcggtgaaac
     9481 tgacctcagc gatgcagtgc gtactgcggt tatcaacaaa cgcgcaggcg gaatggggct
     9541 gattcttgga cgtaaggcgt tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc
     9601 cgtgcaggac gtttatctcg atagcaaaat tactatcgcc tgatgcgctt catacgaaca
     9661 gcactaccac gtatgacgga aaaggcgtta acgccacatc cggcataaaa ccacgcgtac
     9721 tttgttacgg cttcccccgg gaggc
//
LOCUS       DAWDRC010000067         9725 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3533_length_9725_cov_2.227508, whole genome shotgun sequence.
ACCESSION   DAWDRC010000067 DAWDRC010000000
VERSION     DAWDRC010000067.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9725)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3533_length_9725_cov_2.227508"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    284..293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    765..774
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2385..2394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5658..5667
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7320..7329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8127..8136
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2336 a         2684 c         2378 g         2267 t
ORIGIN      
        1 cggcgtaatc gccatcccca tctggctgac cggcagcgtc accaggtcaa agggtttgtt
       61 gtcgttaacc tccaccagca cttcaaaacg ctcgcccatc agcactggca gctcgttcac
      121 cttcaccggt tcaggtagca gaccgccgtc gctggcaatc acataaagcg ggcgattgtc
      181 gctggtggcg aaattgagcg agcgggcatt acagccattg agcaaacgca ggcgcagcca
      241 gccacgcggg gcagcgtgtt gcgggtagat tgcaccgttg gtcnnnnnnn nnntcagcaa
      301 cgtatcgcca aaccaaccca cagcggcggt catcacatcc agttgataat caatctgccc
      361 gtcagcgtta aattttttat cctgaacaat caccgggaca tcatcaatac cccactgttt
      421 tggcagcatt aatttcagga tctcgtcatc ttcaatcaca accaaaccag ccagccccat
      481 cgccacctgt cgcccggtct tgccatgttg atgtggatgg aaccagcagg tagcggcagg
      541 ttgatcaacg ttcaacgtca ccgagcgctt gccacctgac ggaataattc cctgcgggcc
      601 gccgtccact tcacccggta cttccagccc gtgccagtgc aacgtcgtct cttccgtcag
      661 ttggttgtag atatcaaccg ttaccgcttt gccgcgctgt aatttcaccg ccggccccag
      721 cagattgccg ttatagcccc aggtagttgc ggttttcccg ccaannnnnn nnnntcccgc
      781 caaaggtgga ctgacctgcg ccaatagtta actgaatgcg attacgggca tcggtcgtga
      841 gcaaatcagg gattggtaac gttgggcgtt ccgccgcaaa tactgcgcgg ctccacagcg
      901 gcaaggctga agccacaccc agcgcgacgg aatatttcaa gaaatcacga cgttgcatag
      961 ttatttcctt attcttaagc aggcgaaaca aaatcaaacg ttgagcatag tccttcccct
     1021 tacgggaagg tcaagccgca gacacaattt aatcgacaat aatggtcgtc gcctcgctcg
     1081 cagtgtgcta acgtttatct tctttaagcc cctggtagaa gcaatgaaga cgtttttcag
     1141 aacagtgtta ttcggcagcc tgatggccgt ttgcgcaaac agttacgcgc tcagcgagtc
     1201 tgaagccgaa gatatggccg atttaacggc agtttttgtt tttctgaaga acgattgtgg
     1261 ttaccagaac ttacctaacg ggcaaattcg tcgcgcgctg gtctttttcg cccagcaaaa
     1321 ccagtgggat ctcagtaatt acgacacctt cgacatgaaa gccctcggtg aagacagcta
     1381 ccgcgatctc agcggcattg gcattcccgt cgctaaaaag tgcaaagccc tggcccgcga
     1441 ttccttaagc ctgcttgcct acgtcaaata atccctgata cctttttgta gaaatattga
     1501 ccgtgcatcc gcggtcaatg ttagctatta tgttgcgcca tttttttacg ggtgttaaca
     1561 aaggaggtat caacccatgg ccgaaaaaaa acagtggcat gaaacgctac acgaccagtt
     1621 tgggcagtac tttgcggtag ataacgttct gtatcatgaa aagaccgatc accaggatct
     1681 gatcattttt gagaacgccg catttggtcg cgtaatggcg ctggatggtg tagtacaaac
     1741 caccgagcgc gacgagttta tctatcatga gatgatgacc catgttccgc tactggccca
     1801 cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac ggtgccatgc tgcgtgaagt
     1861 aacccgacat aaaaacgttg agtcaatcac gatggtggaa atcgatgcgg gtgtcgtgtc
     1921 gttctgccgt cagtatcttc ccaaccataa cgccggtagt tacgacgatc cgcgctttaa
     1981 actggtgatc gacgatggtg tcaatttcgt taatcaaacc aaccagacct ttgatgtcat
     2041 tatctccgac tgcaccgatc ctatcggtcc cggcgaaagt ctgtttactt ctgcatttta
     2101 tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc gtcgcacaaa acggcgtctg
     2161 ctttttacag caggaagagg ccatcgacag tcatcgcaaa ctcagccatt acttcagcga
     2221 tgttggcttt tatcaggcgg cgatcccgac ctattacggc ggcatcatga cctttgcatg
     2281 ggcgacagat aacgacgccg tacgccatct ctcaaccgaa attattcagg cgcgttttct
     2341 cgcttctggc ctgaaatgcc gttattacaa tccggcagtc catannnnnn nnnncagctt
     2401 ttgccttacc ccagtatctg caagatgcac tggcttcaca gccgtcctaa ggagaagata
     2461 agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt
     2521 gtatttacga tatctgctac gccaaaacta ccgaagagcg cgacggttat attgcttata
     2581 tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta
     2641 tcggggccaa tattcttaac atcgcccgcc aggattacga gccacaaggt gccagcgtca
     2701 ctattctggt gagtgaagaa ccggtcgacc cgaaacttat cgacaaaaca gaacatcccg
     2761 gcccactgcc agaaacggtc gttgcccatc tcgataaaag tcatatttgc gtacatacct
     2821 acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta
     2881 cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg
     2941 atattgtgac tattgattat cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc
     3001 actttatcga ccatgagatt aattcgattc agaactttat gtccgaagat atgaaggcgc
     3061 tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc aagatgttgc
     3121 ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca
     3181 gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc
     3241 gcaatatgcc cgctgtttaa cgtctcaggc ggagcacgga ggctccgcct gatttactta
     3301 cttctgctgc acgaaattac ggtaagccgc caccacctga agaaaatcct caacgccgca
     3361 cagcgacaag ctctcttcat cgtagtagtt catcccctct tccatttcat cgccagcgaa
     3421 ttccagttga ttggcgcgaa ccatcacctc ttcaccatcc atccatagcg tgtattcatg
     3481 ccctgcccgt tgccaggaac gttcgctacc tttcaatgcg tgcgccgctt gttctacttc
     3541 atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg
     3601 ccccatggac atgcgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat
     3661 agtgtgttcc tctgctcctg gcaatgcgcc gaaatcgcat tgcgccattg atgtaattat
     3721 cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg
     3781 gaatctcatt caccgggcat tgtgttgttt atgcgcagcg cgtgcgctta cttattaaac
     3841 cgcagtctgg aaaatcaccc cgtccgcttt ttcggtgtac tgagaaagct ggtcgaagtt
     3901 cagataacgg taagtatcaa cggctgtttt atctacctgc gccacgtagg tctggtactc
     3961 ttccggcgtc ggcagtttgc caatcagcgc cgcaacagca gccagttccg cagaagccag
     4021 gaagacattc gcgccagtac ccaaacggtt cgggaagtta cgggtagagg tggaaaccac
     4081 cgtcgcaccg tctgctacac gcgcctggtt acccatacac agggaacagc cagggatctc
     4141 gatacgagca ccactcttac cgaagacgct gtagtagcct tcttcggtca gctgcgcagc
     4201 gtccatacgc gttggcggtg ccacccacag gcgggtcggc aactgacctt tgtgcgcatc
     4261 cagcagttta ccagcagcac ggaagtgacc gatgttagtc atgcaagaac cgataaatac
     4321 ttcgtcgatc ttctcgccct gtaccgcaga aagcggacgt gcgtcatccg ggtcgttcgg
     4381 agcacagagg attggctctt taatatccgc cagatcgatg tcgatcactg ccgcgtattc
     4441 cgcatctgca tcggcttcca gcagctcagg attcgccagc catttttcca tgccctgaat
     4501 acgacgctcc agggtacgac gatcgccgta accttccgcg atcatccact tcagcaggac
     4561 gatgttagag ttcaggtatt caatgatcgg ttctttgttc agcttgatgg tacaaccggc
     4621 agcagaacgc tcggcagacg catcggtcag ctcaaatgcc tgctcaactt tcagatccgg
     4681 cagaccttca atttccagaa tgcggccaga gaagatgttt ttcttacctt tcttctcaac
     4741 ggtcagcaga ccttgtttga ttgcatacag cggaatggca tgtaccagat cgcgcagggt
     4801 gatacccggc tgcattttgc ctttgaagcg taccagaacg gattctggca tatccagcgg
     4861 cattacgcct gttgcggctg caaatgccac cagaccagaa cccgccggga aagagatacc
     4921 gatcgggaaa cgggtatggg agtcaccacc ggtaccgacg gtatccggca gcagcatacg
     4981 gttcagccag gagtggatta cgccgtcacc cggacgcagc gacacaccgc cacggttcat
     5041 aatgaagtcc ggcagcgtgt ggtgcgtgtt cacgtcaact ggcttcggat acgccgcggt
     5101 gtggcagaaa gactgcatca ccaggtcagc cgagaagccc aggcacgcca ggtctttcag
     5161 ttcatcacgg gtcatcgggc cggtggtgtc ctgggaacct acagaagtca ttttcggttc
     5221 acagtacgcg cccggacgaa tgcctttcac gccacaggct cggcccacca ttttctgcgc
     5281 cagcgagaag ccacggtcgc tctcagcgac atctttcgcc tgacggaaca catcgctgtg
     5341 cggcaggcca agtgcttcac gcgctttggt ggtcaggcca cgcccgataa tcagcggaat
     5401 acggccgcca gcacgcactt catcaatcag cacgtcggtt ttcagttcga aggttgccag
     5461 cagttcgccg gtttcgtggt tacgcacttc acctttgtac gggtaaacgt caattacgtc
     5521 gcccatgttc aggttagaga cgtcgacttc gattggcagt gcacccgcgt cttccatcgt
     5581 gttaaaaaag attggtgcaa ttttaccgcc gaggcacaaa ccaccgccgc gtttgttcgg
     5641 cacatgtgga atatcatnnn nnnnnnnatc gcccataaac cacagaacgg agttcgtggc
     5701 ggatttacgc gatgaacccg tacccacaac gtcaccgacg tacgccagcg ggaaaccttt
     5761 ctgttgcaga gcttcaatct gtttgatcgg gccaacaaca ccaggctgat ctggctcaat
     5821 accttcacgg gcgtttttca gcatcgccag cgcgtgcagt gggatatccg ggcgtgacca
     5881 cgcatctggt gccggagaga ggtcatcggt gttggtttcg ccagtgactt tgaagacggt
     5941 aacggtcagt ttttcagcca gcgccgggcg attcaggaac cattcggcat ccgcccagga
     6001 ctgcattacc tgcttcgcat attcgttgcc tgctttggct ttctcttcta cgtcatagaa
     6061 gttatcgaac atcagcagcg tgtgagaaag tgctttggca gcgatcggtg ccagtttggc
     6121 atcatccagc gcgtcgatca gcggatgaat gttgtaacca ccctgcatag tgcccagcag
     6181 ttcaatggct ttttccggag tcagcagcgg ggatttagct tcgcctttgg cgacagcagc
     6241 caggaagcct gctttgacat aggcggcttc atcgacgcca gggggaacac ggttggttaa
     6301 cagatctaac aggaattctt cttcgcccgc gggcgggttt ttcagcagct ctacaagtgc
     6361 ggccatttgg tttgcatcca ggggtttggg cgcaatcccc tcagcggcac gctcagctac
     6421 gtgcttacgg tattcttcta gcacgacggt tctcctcgct ctcattgtca tagtgcggca
     6481 ggcgattctc ttcacgctcc tgtgagacag cagtttgtag ggtaaatgcc cagtaccgca
     6541 acgggcagaa tagcaggatt ttgacgatgt gttaatctgt ttacaaaaaa gcaacataaa
     6601 aaagtggctg aatcgttaag gatagtctag agaatgtttc cctccgtaaa aacttcgtta
     6661 attcgcatgg tgataatcac ctttctcaac gaatcccaag attagtcaaa atttaaacta
     6721 cggcctcttt atactcggat tcacagcacc tgcgggtggc agttcgccga ccattgcgat
     6781 ttccttgaga tccgaattat gaaaatgact ttgccgttta aaccccatgt gctggcgcta
     6841 atttgcagtg ccgggctttg tgccgcctct gccgggctat atataaaaag ccgcacagtg
     6901 gaagcgcctg tggaagcgca atcgacacaa cagactgcgc ctgacatctc cgcagttacg
     6961 cttcctgcaa cggtttccgc gcctcccgta acgcctgccg tcgtcaaatc cgcattcagc
     7021 actgctcaaa tagatcaatg ggttgcacct gtcgcgctat accccgactc tctgctttca
     7081 caagtgttaa tggcatcaac ctatccggca aacgttgctc aagcagtgca atggtcgcac
     7141 gataatccac ttaaacaagg cgatgctgct attcaggctg tatctgacca gccctgggac
     7201 gccagcgtca aatcactggt ggcctttcca cagttgatgg cattgatggg cgaaaacccg
     7261 caatgggtgc aaaacctggg cgatgctttt ctggcgcagc cgcaggacgt gatggactcn
     7321 nnnnnnnnng actcggtaca gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag
     7381 tcatcaactg aacaaaagat catcgcgaca acgaaaaaag tcgtaccggt aaatcagccc
     7441 gccaacgccc ctgccacaca atcaaatacc gtttctacgt ccagccccgt cgtcgcagaa
     7501 cctgcaccga ccgtaataac cattgagcca gccaatccgg atgtggttta tattccgaac
     7561 tacaacccaa acgtggttta cggcagttgg gccaataccg cttatccgcc agtttatctg
     7621 ccgccgccag ccggagaacc gtttgttgac agctttgtgc gcggattcgg ctatagcatg
     7681 ggcgttgcta ccacatacgc actattcagc agcatcgact gggacgacga cgatcatgac
     7741 catcatcatc atgacgatga tgattatcat caccacgatg gcggtcatcg tgacggtaat
     7801 gactggcaac acaacggcga caacatcaat atcgacgtca acaatttcaa ccgtatcacc
     7861 ggtgagcatc ttactgataa gaatatggca tggcggcaca atccaaacta ccgtaatggt
     7921 gtgacctatc atgatcagga tatggcaaag cggtttcatc aaaccgatgt caacggcgga
     7981 atgagcgcca cgcagttacc tgctccaaca cgcgacagcc agcgtcatgc ggcagcaagt
     8041 cagtttcagc aacgaacaca cgccgccccc gtcattacgc gagataccca acgtcaggca
     8101 gcggcacagc ggtttaatga agcggannnn nnnnnngctg aacactatgg gagctatgac
     8161 gacttccgcg acttcagccg tcgccagcca ctgacccagc aacaaaagga cgccgctcgt
     8221 cagcgttatc agtcggcttc tcctgagcag cgccaggcag tccgcgagaa aattcaggct
     8281 aacccgcaga atcagcagag aagagaagcg gcacgtcagc gtattcagtc cgcgtcgcct
     8341 gagcagcgcc aggcggttcg cgagaaaatg cagactaacc cgcagaatca acagcaaaga
     8401 gatgcagcac gtcagcgtat ccagtctgcc tcgcctgagc agcgtcaggt ttttcgagag
     8461 aaagttcagg agagccgccc gcagcgtcta aacgacagta accatactgc ccgattgaat
     8521 aatgagcaac ggtcagcggt acgcgaacgg ttgtctgagc gtggagcaag gagactggaa
     8581 aggtaaattg caggcgtaaa aaagcggcgt tgttagccgc ttaagactga tgacaaacat
     8641 cgaatcgccc gatgcgcaag cttatcgggc ctacacaagc catgcaatat attgaatttg
     8701 catgattttg taggtcggat aaggcgttta cgccgcatcc ggcatcaacc acgagcactt
     8761 tatcaacaat ctgaagcggc cagaatagcc gcttcttaat ttgcaggatg ttccggcaaa
     8821 cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta ccttcgaaca
     8881 cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcatggatg gtcagtgcga
     8941 tgtcttcagc gtcacaaccc atttcgattg ccaggccgat ttcacccagc agctcaccgc
     9001 cgttagtacc gacaattgca ccaccgatca cacggtgaga ttctttgtcg aaaatcagct
     9061 tggtcatacc gtctgcgcag tcggaagcga tagcacgacc agaagcagcc cacgggaagg
     9121 tggcggtttc gtagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg
     9181 caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag taatgttttt
     9241 taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcattg
     9301 gctgaccgac gatatcaccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt
     9361 caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt
     9421 taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagcgg
     9481 gtgctttttt gccttccatc gtcacataaa tgccgtcttc tttcgcttca acggcggtaa
     9541 ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtaaag actttaacga
     9601 tgtctttatc agctgccggg atgacctggt cgaacatttc aaccacgtca atctgtgaac
     9661 ccagcgcgtg gtatacggtg cccatttcca gaccgatgat accgccaccc attaccagca
     9721 ggcgt
//
LOCUS       DAWDRC010000068         9719 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3540_length_9719_cov_2.143522, whole genome shotgun sequence.
ACCESSION   DAWDRC010000068 DAWDRC010000000
VERSION     DAWDRC010000068.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3540_length_9719_cov_2.143522"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1680..1689
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4157..4166
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5864..5873
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6380..6389
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9578..9587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2187 a         2551 c         2709 g         2222 t
ORIGIN      
        1 atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct gattggtttt
       61 tccggcagcc cgtggacgct ggcgacctac atggtggagg cggcagcagc aaagcgttca
      121 ccgtgatcaa aaaaatgatg tatgccgatc cacaggcgct gcacgcgcta ctcgataaac
      181 tggcgaaaag cgtcactttg tatctgaatg cgcagattaa agccggtgct caggcagtca
      241 tgattttcga cacctggggc ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct
      301 attacatgca taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccag
      361 tcacgctgtt tactaaaggc ggtggacagt ggctggaagc gatggcagaa accggttgcg
      421 atgcgctggg cctcgactgg acaacggaca tcgccgatgc gcgccgtcgc gtgggcaata
      481 aagtcgcgct gcagggtaat atggacccgt cgatgctgta cgcgccgcct gcccgcattg
      541 aagaagaagt ggcgtctata cttgccggtt tcggtcacgg cgaaggccat gtctttaacc
      601 ttggtcacgg cattcatcag gatgtgccgc cagaacatgc aggcgtgttt gtggaggcag
      661 tgcatcgctt gtctgaacag tatcaccgct aaggagcgat tatggatctc gcgtcattac
      721 gcgctcaaca aattgaactg gcttcttctg tgatccgcga ggatcgactc gataaagatc
      781 cgccggatct gatcgccgga gccgatgtcg gatttgagca gggcggagaa gtgacgcgag
      841 cggcgatggt gctgctgaaa tacccctcgc ttgagctggt ggagtataaa gtggcccgca
      901 tcgccaccac catgccttac attccaggtt ttctctcgtt ccgcgaatat cctgcgctgc
      961 tggcagcgtg ggagacgctg tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga
     1021 tctcgcatcc tcgccgtctt ggcgtagcca gccattttgg cttattggtg gatgtgccga
     1081 ccattggcgt ggcgaaaaaa cggctctgcg gtaaattcga accgctctcc agcgaaccgg
     1141 gcgcgctggc cccgctgatg gataaaggcg agcagctggc ctgggtctgg cgcagcaaag
     1201 cgcgctgtaa cccgttgttt atcgctaccg gccatcgggt cagcgtggac agcgcgctgg
     1261 cgtgggtaca acgctgcatg aaaggctatc gtctgccgga gccaacgcgc tgggcggacg
     1321 cggtggccgc ggaacgtccg gcgttcgtgc gctatacagc aaatcagccc taattcaggt
     1381 aaactgcgga taatttccgt atttgagaac tcaacatgtt acaaaaccca attcatctgc
     1441 gtctggagcg cctggaaagc tggcagcatg tcacttttat ggcctgctta tgcgaacgca
     1501 tgtaccccaa ttacgccgta ttctgtcagc aaaccggctt cggcgatggg cagatttacc
     1561 gccgcattct cgatctcatt tgggaaacgc tgaccgtcaa agacgcaaaa gtaaatttcg
     1621 acagccaact ggagaaattt gaagaagcga ttccttcagc cgacgatttc gatctgtacn
     1681 nnnnnnnnnc ttgcgtggcg ttaagtgaac tggtccattc gcgtttaagt ggcgaaacgc
     1741 tcgaacacgc ggtggaagtg agtaagactt ccatcacgac cgtggcgatg ctggaaatga
     1801 ctcaggctgg tcgtgaaatg agcgatgaag agctcaaaga aaacccagct gtagagcaag
     1861 aatgggacat tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg
     1921 agctgataaa aggccttagg gcagacctgc gtgaggcggg tgagagcaat attggtataa
     1981 tttttcagca ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct
     2041 tccctttcgc cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg
     2101 tgtatgcagg agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa
     2161 acacattgta aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag
     2221 cagaactgtc caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg
     2281 agtctctgaa agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc
     2341 accgcgctga gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag
     2401 ctaacgtacc ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg
     2461 tggcagtgaa cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat
     2521 cattgatgct ggcaggcgct ctgtttctca ctgcctgtag tcacaactct tcacttcctc
     2581 cctttaccgc cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca
     2641 gcggcgataa tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga
     2701 cgcgagagta tcgctggcag ggcgataacc ttacgctcat caatatcaat gtttacagca
     2761 aaccgccggt gaacattcgc tcgcggcgat ctgagcttta tgcaacgtga atccgatggg
     2821 gaaaagcagc agctttctaa cgaccaaatc gatttatacc gttatcgtgc tgatcagatc
     2881 cgccagatta gcgatgcctt acgtcagggg agagtcgtgc tgcgccaggg gcgctggcat
     2941 gcgatggaac agaccgtgac cacctgcgaa gggcaaacca ttaaacctga tttagattcg
     3001 caggcgatag cgcatatcga gcgccgccag agccgctctt ctgttgatgt cagcgtggca
     3061 tggctggaag cgcccgaagg ttcgcaatta ctgttggtgg caaactctga tttctgtcgc
     3121 tggcaaccca acgagaaaac gttctgattt accagtggcc catacccata tgaccgccac
     3181 caccgcagcc gccgtagccc atgcccattc ccattccggc tccgcgcgga atacccgctt
     3241 cagccatcgc aatatcccgt ttcacccgta actcatctaa cgactgacgc aaattctcca
     3301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
     3361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
     3421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
     3481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
     3541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
     3601 tcttacttcg ggtattgcat ctttcgtgcc aacggcgaaa cgctgatatg acgggtaatc
     3661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
     3721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
     3781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
     3841 agactcctta gctaaatggt taggcgcgat cctccccgtg gtcattgttg ggctggtggg
     3901 gctgtttgcg gtgacagtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
     3961 gctgctggaa aaaggcagtg tacttatccg tgctctcgaa tccggctcgc gcgtcggcat
     4021 ggggatgcgc atgcatcatg cgcagcagca ggcattactg aaagaaatgg ccgggcagcc
     4081 tggtgtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
     4141 cggcatggtg ggaaaannnn nnnnnnggcg agcctgttct ggaaatttat cgccagtttc
     4201 aaccgatgtt tgctgctgga atgcaccgga tgcgccatat gcagcaatat gccgcgacac
     4261 cacaagcaat ttttatcgct ttcgacgcca gtaacattgt gagtgctgaa gatcgtgagc
     4321 agagaaacac cctgattatc ctcttcgctc tggcgacggt cttgctggca agcgtgttgt
     4381 cattcttctg gtatcgccga tatctgcgct cgcggcagct attgcaggat gaaatgaagc
     4441 gcaaagagaa gctggtggca ctggggcatc ttgcggcagg tgttgcccac gaaatccgta
     4501 acccactttc ctcgattaaa ggactggcga aatactttgc cgaacgcgcg ccagcagggg
     4561 gagaagcgca tcaactggcg caggtgatgg cgaaagaagc cgaccgttta aaccgcgtgg
     4621 taagcgagtt gctggaactg gttaagccaa cgcatctggc tttgcaggcg gtggatctca
     4681 acacgctgat taaccactca ttacagctgg taagccagga tgcaaacagc cgggagatcc
     4741 agttacgctt taccgccaac gacacattac cggaaattca ggccgatccg gacaggctga
     4801 ctcaggtcct gttgaatctc tatctcaatg ctattcaggc gattggtcag catggcgtga
     4861 ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat cagcgttacc gacagcggta
     4921 agggaattgc ggcagatcag cttgaagcca tcttcacccc gtacttcacc accaaagccg
     4981 aaggcgccgg actggggctg gcggttgtgc ataatattgt tgaacaacac ggtggtacaa
     5041 ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac cctttggctt ccggtcaata
     5101 ttacgcgtaa ggacccacaa ggatgacgca cgataatatc gatattctgg tggtggatga
     5161 tgacattagc cactgcacta ttttgcaggc tttgctgcgc ggctggggct ataacgtcgc
     5221 gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg gaacaggttt ttgatcttgt
     5281 gctttgcgat gtacgaatgg cagagatgga tggtatcgcc acgctaaaag agatcaaaac
     5341 attaaacccc gcgattccgg tgctgattat gaccgcgtac tccagcgtcg agacggcggt
     5401 agaggcgttg aaaaccgggg cgctggatta tctcatcaag ccgctggatt tcgataacct
     5461 gcaggcgacg ctggaaaagg cgctcgcgca tacacacagt gttgacgctg aaacgcctgc
     5521 ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg gcgatgcaac acctgctcag
     5581 tgaaatcgcc ctcgtcgcgc catcggaagc cacagtgctg atccacggcg attccggcac
     5641 cggtaaagag ctggtcgcca gggcgattca cgccagtagc gcacggagtg aaaaaccact
     5701 ggtaacgctc aactgtgcgg cactcaacga atccttgctg gaatctgaat tgttcggtca
     5761 cgaaaaaggg gcgtttaccg gagccgataa acgccgggaa gggcgctttg ttgaggcgga
     5821 cggcggcacg ctgtttctcg atgaaattgg cgatatctcg ccgnnnnnnn nnnggagcgc
     5881 gaagttcagc gtgtcggcag caaccagacc atctcggttg atgtccggct gattgcggcg
     5941 actcatcgcg atcttgccgc agaggtgaat gccgggcgtt ttcgccagga tctctactat
     6001 cgcctgaacg tggtggcgat tgaagttccg tcgctgcgtc agcggcggga agatattcct
     6061 ctgctggcaa accattttct tcagcgcttt gccgagcgta atcgtaaggc ggtaaaaggt
     6121 tttacgcccc aggcgatgga tctgctgatt cactacgact ggccgggaaa tattcgtgaa
     6181 ctggaaaaca cggtggaacg ggcggtggtg ctgctgaccg gggaatatat ttccgaacgc
     6241 gagctgccgc tggcgattgc gagtacgccg atcccgctgg tacaaagtca ggatattcag
     6301 ccgctggtgg aagtcgaaaa agaggtgatt cttgcggcac tggagaaaac gggcggcaac
     6361 aaaaccgaag ccgcccgtcn nnnnnnnnna atggatatcg gtcattaagg cataagcgcg
     6421 tttctgcgct tctgccacgg tatgacccag cgcggtgacg cacagtacgc gcccgccgct
     6481 ggtcactacc tgctcgtcat ccgccagttt cgtgcccgcg tggaacactt tgccgtcaga
     6541 cacttcttcc agcggcaggc cgtggatcac atcgccggtg cggtaatcgc ccggatatcc
     6601 acccgcagcc atcaccacgc cgagagaagc gcgttcatcc cactcggacg ttttctcgtc
     6661 cagcttgcct tcacaggccg ccaggcaaag ctcaaccaga tcggacttca tgcgcagcat
     6721 aatcggctgg gtttccggat cgccaaagcg gcagttaaat tcaataacct tcggattgcc
     6781 ctgtttgttg atcatcaggc ccgcgtagag aaaaccggtg taggtgttgc cttctgccgc
     6841 catgcctttc acggttggcc agatgattcg ttccatcgtg cgctgatgaa cctcatcggt
     6901 caccactggc gcaggggagt aagcccccat cccgccggtg ttcggtccgg tatctttatc
     6961 acctacgcgt ttgtggtcct gactggtggc catcggcagc acatgttcgc cgtccaccat
     7021 cacgataaag ctcgcttctt cgccgtcgag gaactcttcg ataacgatgc gatgccccgc
     7081 gtcgccaaaa gcgttgcccg ccagcatatc gtgaacagcc gcttccgctt cttcctgcgt
     7141 catcgccacg ataacgcctt tcccggcagc cagaccgtca gctttaatga cgattggcgc
     7201 gcctttctca cgcagatacg ccagcgcagg ttctacctcg gtgaagttct ggtattccgc
     7261 cgtaggaatg ttatggcggg ccaggaaatc ctttgttaac gccttccggc ccttcagctg
     7321 cgcggcacct gcggttgggc cgaagatttt cagcccggcg gcgcggaagg tatccaccac
     7381 gcctttcacc agcggcgctt ccgggccgac gatggttaga tcgatctttt cgttttgcgc
     7441 gaaatccagc agcgccggga tatcggttac gccgatagcg acgttctgta gggcgggttc
     7501 cagcgcagtg cctgcgttac ccggagcaac aaaaacagtt tcaaccagcg gcgactgggc
     7561 cgctttccag gccagcgcgt gctcgcgccc gccgttacca atcactaata ctttcatctg
     7621 tcgctccatt aatggcggaa gtggcgcatg tcggtgaaga gcatcgcaat accgtgctcg
     7681 tcggcggcgg caatcacttc gtcatcacgg atagaacctc caggctggat tacacaggtc
     7741 acacccgcag cggcggcggc atcaataccg tcgcggaacg ggaagaacgc gtcagaagcc
     7801 attgacgaac ctttcacttc caaaccttca tcggcagctt tgataccggc gattttcgcg
     7861 gagtacacgc ggctcatctg gcctgcgcca ataccgatgg tcatattgtt tttggcatag
     7921 acgatagcgt tggatttcac gaacttcgcc actttccagc agaacagcgc atcacgaagt
     7981 tcctgttcag tcggctgacg tttggtgacg acgcgcagtt cttccgcacc gaccatcccc
     8041 agatcgcgat cctgaaccag cagaccgccg ttcacgcgtt tgaaatcaag acccggaata
     8101 cgttcgcccc actgaccgca ggtcagaacg cgtacgttct gcttggcggc ggtgattttc
     8161 agggcttctt cgctggcgga cggcgcaata atcacttcga cgaactgacg agaaatgatg
     8221 gcctgcgcgg tttcagcatc cagttcgcgg ttaaaggcaa tgatgccgcc gaatgcggag
     8281 gttgggtcgg ttttgtacgc gcgatcgtaa gcatcaagaa tggaattgcc gatagccacg
     8341 ccgcaagggt tggcgtgttt cacaatcaca catgccggct cggcgaactc tttcacgcac
     8401 tccagcgccg catcggtatc ggcgatgttg ttataagaga gggctttacc ctgaacctgg
     8461 gttgcggtgg caacggaggc ttctttcaca ttctcttcta tatagaaggc agcctgctgg
     8521 tggctgttct cgccgtaacg catatcctgc ttcttaatga agttcaggtt cagcgtgcgt
     8581 gggaagcgac cggcggcttc tttgttttca ccgtggtaag ccggaaccat gctgccgaag
     8641 tagttcgcaa tcatgctgtc gtaggcggca gtgtgttcga aggctttgat ggcaaggtcg
     8701 aaacgggttg caagcgtcag cgatccttcg ttggcatcca tctctttaat aatggcgtcg
     8761 tagtcgctgc tcttcaccac gattgcgaca tctttatggt tcttggcggc ggagcgcacc
     8821 atcgttgggc cgccgatatc gatgttctca accgcatctt ccagcgagca gccttcacga
     8881 gcgacggtct gggcgaacgg atacaggtta acaaccacca tatcgatagg ctggatctga
     8941 tgttcttcca taatggcatc gtcctggccg cgacggccca gaatgccacc atgtactttc
     9001 ggatgcaggg tcttcacgcg tccatccatc atctccggga aaccggtgta atcggaaact
     9061 tcggttaccg gcagaccttt ttctgctaac agacgggcag tgccccctgt agacagcagc
     9121 tccacaccgc gtgcggaaag tgcctgggcg aattcgacga taccggcttt gtcagaaaca
     9181 ctgagcagag cgcggcggac tggacgacgt tgttgcatgg taaatcccct ggatttgact
     9241 attacagaga gcgttagctg aatttttcgc gaaaaactca gctaacgccc ctaacggggc
     9301 atccttattt ttcgcccgca ttgtaacgaa aacgtttgcg caacgctcgc gaatttttct
     9361 ctttcaatgg tgatcacaat tttgacagtg gctaccgtgg gaaaaataca gaaattacat
     9421 tgatgattgt ggataactct gtgcgtaaaa aggtataaag tggggttttg ctggggaatg
     9481 cagcagtcag tcatttttat gcaatttttc tattgcggcc tgcggagaac tccctataat
     9541 gcgcctccat cgacacggcg gatgtgaatc acttcacnnn nnnnnnngag aattccggca
     9601 gagaaagcaa aaataaatgc ttgactctgt agcgggaaag cgtattatgc acaccccgcg
     9661 ccgctgagaa aaagcgaagc ggcactgctc tttaacaatt tatcagacaa tctgtgtgg
//
LOCUS       DAWDRC010000069         9673 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3553_length_9673_cov_2.863901, whole genome shotgun sequence.
ACCESSION   DAWDRC010000069 DAWDRC010000000
VERSION     DAWDRC010000069.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9673)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9673)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3553_length_9673_cov_2.863901"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6774..6783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2528 a         2534 c         2459 g         2142 t
ORIGIN      
        1 gtggcgcatt atctgtggaa ccgctttggc agctcccacc gcgtgcgttt tgtcgctatt
       61 aatttcgaac cggtcgtcgg ggaaattctc gagaaaatcg acgacggtca gatgggcgtt
      121 atcctcaagc gtatgatggt gcgtgccgcg tccaaagtgg cagaacgtta cggcgtacag
      181 gcgctggtta ccggtgaagc gctcggccag gtgtccagcc agacgctgac caacctgcgc
      241 ctgattgata acgtctccga tacgctgatc ctgcgtccgc tgatctctta cgacaaagag
      301 cacatcatca acctggctcg ccagattggc accgaagact ttgcccgcac gatgccggaa
      361 tattgcggcg tgatttccaa aagcccgacg gtgaaagcgg ttaaatcgaa gattgaagcg
      421 gaagaagaga agttcgactt cagcattctc gataaagtgg tggaggaagc gaataacgtt
      481 gatatccgcg aaatcgccca gcagaccgag caggaagtgg tggaagtgga aaccgtcaat
      541 ggcttcggcc cgaacgacgt gatcctcgat atccgttcta tcgatgaaca ggaagataag
      601 ccactgaaag tcgaagggat tgatgtggtt tctctgccgt tctataaact gagcaccaaa
      661 tttggcgatc tcgaccagag caaaacctgg ctgttgtggt gtgagcgcgg ggtgatgagc
      721 cgcctgcagg cgctctatct gcgcgagcag ggctttaaca atgtgaaggt gtatcgcccg
      781 taatatgtgg ttgtaacgtc gcatctggtc agatgcgacg cttaccgcat ccgacactac
      841 tcgtaataat tataaatccc tgctgccatc accagttgtg atgccacttc atgggctttc
      901 tcgcgcccaa ccagcaggtc gatcattttc agaccaaagt cgatagctgt acctggcccc
      961 tggctggtca gcaattttac ccgtgcatcc cagacgacgc gcttgtcctg ccattgttcg
     1021 gcgggtattt tgtctttcag cgtcgggaag ccagtcatat tgccaatcgg gaagatatcg
     1081 tgcgggacca gcatggtggc tggcgcggcg caaatagccg cgacgatacg cccggaacgg
     1141 tggaactgtt taacggtttc aaccagcagg gtgctatcgc gaaaacactc tgcgcctttg
     1201 atgccaccag gcagcacaat cacgtcatat tcgccatcag ccacttcaac cagcggcgca
     1261 tccgccagca gcttcacgcc gcgcgagcag gtaatcgcca ggttaccatc gctggcgacg
     1321 ctggcagtgg tgactttgat accgccgcga accagcagat cgatagtggt gacggcttca
     1381 gtctcttcac taccaggggc gaggcaaacc agtgccgatg cgctcatatt cactctcctt
     1441 tctttttacc atttcaaaca ggcgggtgtt ttccggtacg gcaatcccat gcgcgcgggc
     1501 gcggcgtaag agaaaaccat tgatatagtc gatttcggtg tggcgcagcg cgcggatatc
     1561 ctgcaacatc gacgagatat tttccgctgt ggcatcaatc acctgcatca cgtaatcacg
     1621 caaatcttct gctgaagtat gatgcccttc gcgttcgatc actgccgcga cttcttcgca
     1681 tatctgcata atttcttgtt gatgatggcg tagttcacca ttcgggcaat tcaagatggc
     1741 ggtcagtgga ttaatcacac agttgactgc cagcttgcgc cacagctcgg cgcgaatatt
     1801 gttatgccag gcgacgtcag gtaacacggt ttgcaaaata tccgccagat aactgtaatc
     1861 gccgtcctgt tgccgtgccg gaccaatatg cgtgataccg tttgccacat gaataatgac
     1921 attgccgtcg cggcgagctg catgggtggt ggtgcccatc agtaatggct gctgaatgtt
     1981 ttgcaactct tcgatggtgc ccatgccgtt gtgaattaac agtattggcg tggttacagg
     2041 cagtgtggac gcgaggcttt tgacggcatc ggaaacctgc catgctttca gcgtcaccag
     2101 gagcagatcg ctgatggcga gaaaatcggg atcgttggcg gtcagcgatt cgttaaatat
     2161 cgaaccatct gtctcaacca gattcacact acaataaggt tgcggtacgc gcagccagcc
     2221 ctgaacgtcg tgaccctgtt tacaaagtgc tgtaagccat aattgcccta aggcaccgca
     2281 tcccaatacg gtaattttca ttgttcctcc tcacccgcaa ccactccggg tgttcaataa
     2341 ggctatccct taattgtgca tgctgttgcg actatgcaca attaagggat acgtcctggt
     2401 gcaggactgt tggttattta actttgcagg tattatgctt cgcatcaaaa atgaagggag
     2461 aggaaaagat gccatctttc gatattgtct ctgaagttga tcttcaggaa gcacgtaacg
     2521 cggtcgataa cgcgagccgc gaagtggagt cccgttttga cttccgtaac gttgaagcct
     2581 catttgagct gaatgacgcc agcaaaacca tcaaagtgtt gagcgagtcc gacttccagg
     2641 tcaatcagtt gctggatatt ctgcgtgcca agttgctgaa gcgcggcatt gaaggcagtt
     2701 cactggatgt accggaaaat atcgttcata gcggtaaaac ctggtttgtg gaagcgaaac
     2761 tgaaacaggg cattgagagc gcgactcaga agaaaatcgt caaaatgatc aaagacagca
     2821 aactgaaagt gcaggcgcaa attcagggcg atgagatccg cgtaacgggt aaatctcgtg
     2881 atgatttaca ggctgtcatg gcgatggtac gtggtggcga tctgggtcag ccgttccagt
     2941 tcaaaaactt ccgcgattaa tcgcgacaac ctgtgctttg ttcatgtcag atgtggcatg
     3001 aacgcctgat tcggcctaca aaggtttgta actcaggaac ttagtaggcc tgataagcgt
     3061 agcgcagcaa gcaatttcgc ctcgctttta tgcctggcga attgcctgtt caacctcaaa
     3121 gcgattcgtc actttgctgt cgattttcac ataagctgaa tgttcttctt ctgcaatcaa
     3181 cacttctttg atgccttcag tttccagcaa acgcactttt aacgcctcgt ttgcggcaat
     3241 gttcgccggg atttcaatgc gcaagctgct gacatacggc ggttctttca tggtactggc
     3301 gactgccagc cacactgcgg ccagcattgc gccagcgaga aatactccct gaccgtcaaa
     3361 catgccgtca atccagccgc ccagcgaacc gccaatcgcc acgccaagaa actggctggt
     3421 ggagtaaaca cccatcgccg tacctttgta acctgctggc gattctttac tgataagtga
     3481 aggcagaagg gcttccatca aattaaacgc cacaaagaaa agctgcacgc cgaccaccag
     3541 ttgccagaac tgcgtttgtg cgttccacaa cacaatttcc gcaaccacga tcaacccgac
     3601 gcagaagaca aagacttgct tcattttgcg cttaacttca gcgtagataa tgaaaggcac
     3661 gaccgagcca aaggcgatta acatcgttgc caggtagacc ttccagtgtt cagccgccgg
     3721 taaccccgca tcagccatct gtccgggcag ggcaacaaag gtcgacatca gcaaaatatg
     3781 cagacacata atgccaaagt tgagtttcag cagccgcggt tccgccagca ctttactgaa
     3841 actgcctttc accattccgg actcacgatt aagtacgtga gtgctactgt tgggcacaac
     3901 ccaaatggtc aacgcaatgc cggttgttgc cagaatagcg atcatccaga acagcgcgtg
     3961 cagcccaagt ttgtgagtga tgatcgggcc aagcaccatc gcaatggcaa aggtaatgcc
     4021 aaagctcacg ccgataaacg ccatcgcttt ggtgcggttt tgttcgcgcg tgagatcgga
     4081 aagcagcgcc ataacggcag cggcaatcgc accggagcct tgtagcgccc ggcccagaat
     4141 aattccccag atggagtcag agagcgcagc gataacgcta ccggcggcaa acaccgccag
     4201 cccaccgaca attaatggtt tgcgaccaat gcggtctgaa agcaggccaa acggaatctg
     4261 aaaaacggcc tgagtcagac cataaatacc aatggcaata ccgattaatg cttcgctggc
     4321 accttgcaga gccatgccgt acgtggtcag aaccggcaga accatgaaca tgcccagcat
     4381 gcgtaatgaa aatacggtcc ctaaacccca ggtcgcgcgc cgctcacctg gcgtcatttt
     4441 ataatcgttc attaccacct ctgttttaaa ttcgcgacta gtgtaaagcg gcaaacgaat
     4501 ggggtaaaca tgcggatttt ttagtaaata tttcgagagg gatatgattc taatgctaag
     4561 aaaaaaggtg ccgtagcacc tttttaactg agaggttgtg ttaccacaca gcagccagca
     4621 gcgtatgcga gtccggcacc ataaaatcaa cggacatcat caccgagagg gcagtgatgg
     4681 cgatgataga gaaaccgaac agcttgcgcg cccagattct gtcatcagca actttatagc
     4741 cgcgcagagc catgcctaac caccagacgc tcaccgcagc ggcgaccacc agatatttat
     4801 acccagcgta accgccaaga gagagcatca gcgtggcaac ggcaaaggcg atgatataca
     4861 gcgtgatgtg attcttcgcc accgaaatgc cttttaccac tggcaatacc ggaatgtttg
     4921 ccgcctggta atccttaaag cggaaaatgg cgatggcata ggagtgaggc atctgccaca
     4981 gactgaagat agcaagcagg atcgctgcgc cactatcgaa ctcgccggtt actgcacagt
     5041 agccgatcac cggcggcgca gcgccggaga gcgaaccaat caatgtgccg tagactgagt
     5101 ggcgtttcat gtacaggcta taaacgccga cataaaccac aaagcccatc acccccagcc
     5161 agcaggccag cggattcgcg ccaaaccaca gcagcataaa gccagcaata cccagcaagg
     5221 tggcgtacac cagcgagaca gcaggagaga tcaggccttt aaccagcacc cgattcttcg
     5281 tcctttccat ctttctgtcg atatccctgt cgatgtagtt gttaaacaca caacccgacg
     5341 ccacaaccag tgacacccca accagcgtgt agataaacag gggataatca atgctgccct
     5401 ttgaggccag caggaatccc ccaatcaccg agatcaggtt gccaaagatg atgcctggtt
     5461 tcgttacttg caggtattgc ttaaacatca taaccgccgc tcttagtgca tcatcatgtt
     5521 gtagttaagg ttccacataa tccagatgga gcctacaacc aggatagcga tgattagcac
     5581 ggtgaagaca aacgccgtca tgttccagcc ttcatctgat ttggtattca tgtgcaggaa
     5641 gcacaccaga tgcaccagaa cctgtaccac tgccattgcc aggattgttc ccagaattac
     5701 ggccggagag gcagcccctg tcatcaccat ccagaacgga atcaccgtca gaatgatcga
     5761 caggataaag cctgtcatgt aggtttttac gctgccatgg gacgcgccgc tgtgatcggt
     5821 agaatgactc attacatcgc ccccatcaga taaacaacag tgaacacaca gatccaaacc
     5881 acatccagga agtgccagaa caggctcaga cacatgatgc gggtacggtt agtgctggtc
     5941 aggccgcgac gggcgatttg caccatcagc accgccatcc agataagacc ggaagtgacg
     6001 tgcagaccgt gcgtgccgac cagcgcaaag aatgctgaca ggaagccgct gcgatccgga
     6061 cccatgccgt taacaatcag gtgatggaat tcatagattt ccatcccgat aaatccggca
     6121 ccaaacaacc atgtcaacgc cagccaggag atcacctggc ttttgttgtt tttgtacatg
     6181 gcgatagccg ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt ttcaaccaga
     6241 acgaacggca gttcgaaaat gtccttacct gtcggcccac ctgcggtgcc gttcaccaga
     6301 acggcatagg tagcaaacaa gatagagaac agaatgcagt cgctcatcag gtagatccaa
     6361 aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc gtgggcagtc
     6421 gcgtgcgtca aagtatcagt tgccattttt cagccctgcc ttagtaatct catcgaaatg
     6481 ctggttttcc agtttttcga tttctgccac cggcacgtag taatccacgt cctcgtcgaa
     6541 gcttttcacg atccaggtga tgatcatgcc tgcgaagcca acaatcgcca gccaccagat
     6601 atgccagatc atggcgaaac cgaagatggt ggagaaagcc gcaatgacga taccggcacc
     6661 gctgtttttc ggcatatgaa tttcttcata gtggtcaggc tttttgtacg cttcgccttt
     6721 ctctttcatt tcccagaatg catcacgttc gtgaacgtgc ggcacaacgg caannnnnnn
     6781 nnnaaagtta tagaacggag gcggggaaga ggttgcccac tccagcgtac ggccacccca
     6841 cgggtcgcca gtcaggtcac ggttctggtc gcggtcacga atagaaacgt acatctgaat
     6901 aacgaggcag agaataccca gcgcaatcag taccgcaccg ctggctgcaa tcatcagcat
     6961 ggtgtggaac tgcgggtcaa tctgctggct caaacgacgg gtcatgccca tgaagcccag
     7021 cgcatacagt ggcataaagg caacgaagaa gccgatgatc cagaaccaga acgcgcgttt
     7081 accccaggtt tcgttcagtt tgaaaccgaa cgctttaggc caccagtagg tcatccctgc
     7141 gaagcagccg aagaccacgc cgccgatgat cacgttatgg aagtgcgcaa tcaggaacag
     7201 gctgttatgc agaacgaagt ccgcgcccgg tactgccagc agcacgccag tcatcccgcc
     7261 caccgagaag gtgacgataa aaccgatggt ccacagcatc gcagaatgga acacgatgcg
     7321 gccctgatac atggtgaaca gccagttgaa gatcttcacc ccggtcggga tggcgataat
     7381 catcgtggtg ataccaaaga aggcgtttac gttcgcgccc gcacccatcg taaagaagtg
     7441 gtgcagccag acgatgaacg acagcacggt gatacagacg gttgcccata ccagcgaggt
     7501 ataaccaaac agacgtttac gcgagaaggt tgccgcaatt tcggagaaca caccgaatac
     7561 aggcaggatc aagatgtaaa cttccgggtg gccccaggcc caaatcaggt tgatatacat
     7621 catcatgttg ccacccatat cgttggtaaa gaaatgggtg cccagatagc gatccagggt
     7681 caacaacgcg acggtaaccg tcagaattgg gaaggaagca ataatcagta cgtttgcgca
     7741 cagtgatgcc caggtaaata ctggcatctt gaacatggtc atgcccggtg cgcgcatctt
     7801 tagaatggta acgaagaagt tgataccggt aagcgtcgta cctataccgg atagctggag
     7861 actccatatc cagtaatcga caccgactcc cggactgtac tctattcccg atagcggtgg
     7921 ataggccagc cagccggtct gcgcaaattc gcccacgccg agagaaacgt taaccagaat
     7981 cacaccaaca acggtaaacc agaagcttaa gttgttgagg aacgggaacg caacgtcacg
     8041 cgcgccgatc tgcagcggaa ccaccaggtt catcagaccg ataacgaaag gcatcgccac
     8101 gaagaagatc ataatcacgc cgtgcgcggt gaagatctga tcgtagtggt gaggtggcag
     8161 gaagcccgct tcgcccgccg aggcaagagc ctgctggcta cgcatcataa tggcgtcagc
     8221 aaaaccacgc agcaacatca caatcgccac gatgatatac atgataccga gacgtttatg
     8281 gtcaacggag gtcagccact ctttccacag gtaggtccac ttaccgaagt aagtgatcag
     8341 gccaacgagc gccagacctc ccaaaataat gccagcgatc gtaaccatga cgataggttc
     8401 atggaacggg actgcatcaa gtgataattt tccgaacatc tttattcttc ctcaacccct
     8461 ttaatgggcg gattccgcgt ggctcatgtc catgccttcc ataccttcat gtgcgctgtg
     8521 ctcaccttct ggctgggtca tgtccatgct cttaccgtga gccataaact tgttaatcac
     8581 atcggcaaac aagtctggtt tcacgttgga gaaatattcc acctggttgt attcgctagg
     8641 cgcggccagt ttttcgaacg ctgccatgtc agacatgctg ttcggcgact gtttcgcttt
     8701 tgcgacccat tggtcgaatg cggcgcgatc cggtgttgca atagctttga acttcatgcc
     8761 tgagaagccc gggccgctgt agctggcgga gataccgtca taagtaccgg gttcgttggc
     8821 gatcagatgc aggcgagtct gcataccggc catggcataa atctggctac ccagacgcgg
     8881 aatgaagaag gagttcatca cggagttgga ggtcactttg aagtacaccg gagtgttcgc
     8941 cgggaaagcg atttcattca cggttgcaat gccctgttcc gggtagatga agaaccattt
     9001 ccagtccatg gaaaccactt cgatggtaat gggcttctcg tcgtgtgcca gcggcttgct
     9061 aggctcaaga gcgtgagtgg ttttccaggt cagtaccgca aggaagatga tgattaagat
     9121 aggtaccgtc cagaccacag cttccacttt attggagtgt gaccagttcg ggctgtactt
     9181 agcatcttta ttgctcgcac ggtacttcca ggcgaaacca acagccatca agattgcggg
     9241 aataacgaca atcaacatca ggccaaatgc cgtcagtatc agtgaacgtt gctccagacc
     9301 aatctgtcct ttgggatcta acagcgcaga attacagcca ctgagcaata cagtgcctgc
     9361 aaataatgac aaccatccca aacttttatt gtatttcctg agtctcattt aacgacctca
     9421 attccacggg atctggtggc gtttaaagtg tgtgggcatt ttacgggaag gttacattac
     9481 tgtaaacatg attaaattcc tgttacttgg atttggctgt caggtcacat atgttgcaaa
     9541 acatatcaag ttgattttaa gaagctgttg caaaagggcg ttataacgaa aggggagtgc
     9601 agaaggagtt tgggtgggca attggtataa tcaatataaa ataaaacaat tatttaacaa
     9661 ataattatca ttg
//
LOCUS       DAWDRC010000070         9620 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3577_length_9620_cov_1.881129, whole genome shotgun sequence.
ACCESSION   DAWDRC010000070 DAWDRC010000000
VERSION     DAWDRC010000070.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3577_length_9620_cov_1.881129"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1741..1750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2896..2905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3425..3434
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4550..4559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6154..6163
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8499..8508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2545 a         2385 c         2133 g         2497 t
ORIGIN      
        1 tacttttgtt ttgggtgtat ttcaatctgt taaaaaattt ttcgctacac tggcaagcaa
       61 aaatgaaaca ggaataatcg aaatgggatg ttgcgcacag ttaaaataac tcaccgtaaa
      121 taatcatctg ctataaataa tcgctttaat gcaataccag ataagccatt tttaaacaaa
      181 cacttaccgc acaacaaact aattaactac aaagaaaggc aacaatcaac ctaatgttaa
      241 taaaatgttt tacatatatt aaccacttgt tcaatatggg aaatcagtac attattttct
      301 tataaataca gttgaaatca tatgataacc aagaacaatt gattaattgt atttactaat
      361 gaccagacta atatttacct gtttaactga gttgggattg cgtcgtttct ccattaggag
      421 taaagcttta atgtcacctg aagttcacag aataaggaac aggttatgag ctatagcaat
      481 attcttgtcg ccgttgcggt tacaccggaa agtcagcaac tgctggcaaa agcggtatct
      541 atcgccaggc cagtaaaggg acacatcagt ttaattactc tcgcttccga cccggaaatg
      601 tacaatcaat tagctgcgcc gatgcttgaa gatttacgca gcgtgatgca ggaagaaacg
      661 caaagctttc ttgataagtt aattcaggat gcggggtatc ccgttgacaa gacgtttatt
      721 gcctacggtg agttaagcga acatattctg gaagtttgtc gcaaacatca tttcgatttg
      781 gtgatttgcg gtaatcacaa tcacagtttc ttttcgcgag catcctgctc ggcgaaaaga
      841 gtgattacct caagtgaggt cgatgtgctg ttagttccac ttacgggaga ttaaccgcac
      901 ctacgcaagc ttaggaaagg tagcaacttt atcgcgctgc tggctttccg cgcttcgcgg
      961 aactatctgc tttaggtcgc taatgaagca ctcctgccag tggttaatat cgtttttcac
     1021 gataacgtcc agcatttctg catgacggga aatacgttcc gccagcgaca tagtcaatgc
     1081 acgatccagc gcagctgcaa cttcgtcacg atcgtaggga ttaacaatta acgccgacgt
     1141 taactcgttt gccgctcccg caaattgcga aagaacaaga acgcccgggt tggctgggtc
     1201 ctgagcggca acatactctt ttgccaccag gttcatcccg tcacgcagtg gcgtcactaa
     1261 gcccacgtca gagtagcgga atattttcat cagtaattta cggtcaaaat gctgattcaa
     1321 ataataaagc ggcgtccagc ctaattgccc atatttacca ttaattcgtc cggcttcatt
     1381 ttcgagctga tgacgaatat cctgataggc ttgcacatca ccacgagacg ttggcgcgat
     1441 ctgggtataa cgaattttac cgtgatgttg cggatatttt tccagtaacg cttcataggc
     1501 gagaaaacgc tccggcaaac ctttggaata atccagtcgt tcgacagaaa agatattttg
     1561 tacgtttttc agttccgctt tgagttgcgc cagttttggc ggcagtggac cggcagcctg
     1621 tttcgctatt tctttcggct caatgccgat cgggtaaact tccgttcgaa acgctttgcc
     1681 gcaggccgta tggctttttg cgctacgtgt cgtgacgcgg gtcaggttag aaaggcaatc
     1741 nnnnnnnnnn gcaatccagg aacgccagac gatcgttctc cgtctggaaa cccagcaaat
     1801 cataatcaca aagctgttca agcaaggtgt catatgtcgg cagcgcgttg aagatttctg
     1861 gtgtcgggaa aggaatatgc agaaagaaac caatgcgatt attcacgccc cgtttgcgta
     1921 attcatgcgc aaagggcaac aggtgataat cgtggatcca gataatgtca tcgtcttgca
     1981 acagcggcag taatttatct gccagcaacg cagttacgcg tagatagcca tcccaggcag
     2041 gacgctgaaa ttgcaccaga tcgagccgat aatgaaaagc gggccagaga acggcattgg
     2101 agaatttgtt gtagtattcg tcaaggtcct gttcgctgag gttaaaagag gcccacgtaa
     2161 tgttaccctt tttcaccttt tttagcggct gatcctcatt ccctgtttca ccgctccagc
     2221 caaaccacag tccacctgcg gctttcagtg cccccagtat gccaacggca aggccaccgg
     2281 cactggcggc gtgctcgtct ggtggtgcaa tccggttaga tactacgact aaacgactca
     2341 tagtcatcac tcctgttatt ttctcttttt tgttgtaatg cggtggttat catttcaagc
     2401 cagctccaga catccggcac gcccgccagt cgccatgatg cctgagttgc acctgtgcca
     2461 atttttactg acattccgcc cagccggtta acgactgcga agccagattc atcggtcaga
     2521 tcatcgccca gaaatacggg cgttcgcccg ataaagggag cttcctgcat aaaggcagca
     2581 atcgcctcac ctttactggt gcctcgcggt ttgatctcga caacacactt tccctgctgt
     2641 aacgccattt gtggccagat ctgagtaata cgttgcgcta atgtcattaa tgcgtcttca
     2701 tgctgcggag cctgacgata atgcagcgca aaggccatcc ctttcgtctc cagctccgca
     2761 ccgggaaact gggcgatagc cgtatgcagt tgcacgctaa tatcacgcgc aatcgcatcc
     2821 ggcagatgaa cgatatgcgt tttaccattg atgtcacggc gctccgcccc atgcacgccc
     2881 gctaacggga agcgannnnn nnnnngtttt gccagtgcgt caagctccac cattgagcgc
     2941 cctgaaatca atgccaatgc accatcactt gcggttgcca gtagctgaag tccttgcaga
     3001 atagtgtcag gcacgacgac ctgatcggga tgcggtttga tttccgccag cgttccatcg
     3061 agatcaaaaa accaggcata tttcgcggat agttcagggg tttcggttaa cggttctgtc
     3121 acccggttct cctccttctc ttttcttatt gtgttgcgca gaacgcgctt tacagacatg
     3181 taagtataga cagtgtgacg ggggtcgcca tttgcaacga agaacagcca accgttgtgg
     3241 cggctggctg tggtgggaaa aaacgctaaa ttgttgtgga aaaaagcatc agacagtgcg
     3301 tttcgctttt tgcttgtaac ggtcgaaaat caccgctgcc agcaggatta agccgcgaac
     3361 tacgtactgt gcgaaaggag aaatattaag caggttcatg gcgttttcca cggtgcctaa
     3421 aattnnnnnn nnnnttaaga tacccgccac cacatatgag atttttccga tgccgccttt
     3481 cagagaaacg ccacctaaaa cgcaggcgga gatcacaatc agctcatagc caatcgacgt
     3541 cattggctgc ccactggtca tacgtgaagc cagaataatt ccggctatcg ctgataccag
     3601 gccagagaga acaaagataa taattttggt gcgaacaacc ggtacacccg ccagacgcgc
     3661 agcctcttcg ttcccgccaa tcgccagggt attacgacca aaggtggttt tattcagcag
     3721 caagccaaag atgatcaaac acgcgacggt gagccagatt ggcgcaggca gaccaaacca
     3781 gttggcgtaa ccaagggcaa agaagctttc atcttcgata ccgaccgctt taccgtctga
     3841 aatgatgtac gccagacctc gaacaatctg catcgttgcc agtgtcgtga tcagggcatt
     3901 tattttgagt ttggcgataa caaagccatt gaccagacca cagagaatgc ccagcagcaa
     3961 ccccgctgcc acgccaatcc acaggctttc agtcaggttg ataaccaccg cagtggtgac
     4021 acccgcacag gcaattacgg aggcgacaga aaggtcaaag tcaccggaag caaggcagaa
     4081 caacatgcca cacgccacca tccccgacat agaaattgcc aggcccaacc ctttcatatt
     4141 aatgaaggtg gcaaaatttg ggacaaaaat ggcacaggcg ataaaaagca ccgcaaacac
     4201 caccagcatg ccgtactgat cccagatacg cccgaagctg aatgacgact taggtgcgcc
     4261 agaacccgat gtagaaacag aagacatcat actctcctta ctcaggcaac tgcctggctg
     4321 actttaggca tcgcaaggct cagtgcctga cgctcatctg cctgctcgtg taacaattca
     4381 ccggcgattt cgccttcgcg catcaccaca atccggtcgg caacgccgag gacttcaggt
     4441 aagtcgctgg aggcaaacag caccgccaca ccctgcgccg ccagcgcata aatcacgttg
     4501 taaatttcat gcttagcacc aacatcaatg ccacgcgtag gttcatccan nnnnnnnnnc
     4561 tgagagattc ataatcagtt gctcagcgcc cggcgttttg atgttgagcg aacgaatgtg
     4621 gtgatcggca ttgttttctt cccaaccgtt gttgattaca caaccgccga gcacatgttt
     4681 gcgtctggca ctgatgttga tattgtcgcg aacggagtgc acaggaataa tgccttccgc
     4741 tttgcgatct tccgggcaga gcatcatgcc tgcggtaata gcgtggctcg gtttacgaat
     4801 atcgatcggc tgttggtcga tataaacctg accggcggtg atttgcgtac cgccaaacat
     4861 gcctttcatt aattcgctac gccctgcccc gaccagacca aacagaccga caatttctcc
     4921 actgcgaacc gccagactta ttggcgtacg cacgcctggt gctttcacag catcaagacg
     4981 cagccgctcc tcgccataac tacgcggttg ccagccgtag atatcgccaa tgtctcgccc
     5041 gaccatcgcc tgcaccagtg cgtcgtggtc aacctgctgc atatcggtaa aggttttgac
     5101 ataacgccca tctttaaaga cggtgatggc atcgctgagg gcaaatattt cttccatacg
     5161 gtgagaaacg tataagatca cccgcccctc ttttcgcagt tcacgaataa cgcggaaaag
     5221 attgtcgatt tcgcgggcag agagggagct ggttggctca tcaaaggcga taattttggc
     5281 gttacgcgcc agcgctttgg cgatttcaac catctgccac tgaccaatgg agagatattt
     5341 cagcggcgtg tccgggtcaa tatccatacc aagatgttta atttgtaaac ctgcctcata
     5401 attcagcaat gaacgattca caatgccgcc tttatgcggc agctggccga gatagatgtt
     5461 ttccgcgacg gtcatttccg gcacgagatg cagttcctga taaataatcg ccacacccgc
     5521 gttaagtgct gcggtcgtgt cggaaaagga catttcctgc ccattaatca ctacagaacc
     5581 cgtggttggc gcatagtttc cgctgaggat ttttaagaga gttgattttc ctgcgccatt
     5641 ttcacccatc aacgcatgaa cctgaccggc atagcagtca aaactaatat ccgtcagcgc
     5701 cttaacgccg ggaaacgttt taccgatgcc gcgaaatgag agatacgggg tagactgttg
     5761 cataacgtct ccgtgaatca ctggtcgttc acaccccctc tgcatcatgc agaggggaat
     5821 ttttccggca aattacttac cgcctaaacc ttttttctcc agttcttctt taaagttgtc
     5881 acgcgtgatc agcaccacgt cggtaacttc ggtgaatttg gtcggttcaa caccttttgc
     5941 aacccagttg taaagcattt cgctggactt ataaccatgt acgtccgggc ttggcagcag
     6001 ggaaccgtag aagccggttg cctgtgcctt agacagttca ctcaccgcat ccacaccgtt
     6061 aataccgata ccgatgatat cggccgcttt aaagccctga ccttccgtcg cgcgtacgcc
     6121 gcccagcacg gtgctgtcgt tcataccgac aatnnnnnnn nnnagttggc agcgtcaaat
     6181 gcccccggga tgtcgttaga tttggtaggt acctgataaa tttgtttttc cgggaatcct
     6241 gcagctttca gcgcatccat agaccccgta gtacggcgac gagccgtatc cagttcgttg
     6301 gaggtaatcg ccatcaccgc actttctttg acatcccagc cacgtttctg catctcttta
     6361 tacagttcct ggccctgacg ttcgccaatt ttggtcgctg ccatcatcac cagcggaacg
     6421 gtatccattg gcttaccttt ggcgttaaca aactggtcat ccacggcaat gactttcata
     6481 tcgtagccac gcgctttcgc cacgatggca gagccgagtt tcgggtccgg agtacaaata
     6541 acgaaacctt tcgcgccact ggcggccagg ctgtcgatag cgttcaatgt tttttcgcca
     6601 tccggcacgg caatcttaat aacttcaaac cctaaatcct tcccggcttt atcggcaaac
     6661 ttccattcgg tctggaacca cggctcttcc ggttgcttca ccaggaaacc gagcttcagg
     6721 ttctccgcca tagcggattg tgacataacg gctgccagac caatggctgc cagggcttta
     6781 gtaaatttgt gcatggttct ctccagcttt agtgtcgttt tgtgtagggc aaaaacgaat
     6841 gacattcgtt aaattaatcg gaaaacaaag cattaccttt taactaaaag ataagtgact
     6901 gtgttgacat agttttagcg agaaattaat tctccatagg agagcaatat cacatcgcag
     6961 aattacagtg agaacgtgca taaatttagc gggaaaagac ataaagaaaa gccaatttgt
     7021 cagacaaatt gtcgaatgca cagcagatta atccataaga ttagccttga aatccttgtt
     7081 atctttggta cccatgcggg atgtcttctt tttaaccagt caataggcct cattacctgg
     7141 cgttgagttt ttgcaatggt gtaataaccg caacttaaag atgtggaaaa tgcgcgtcat
     7201 tcatttcgcc attaattatc actgtactca ttaattaaca gaacacgtat aatgagagcc
     7261 atatcgcaaa aatgaaaaaa cgttttataa aatcattgct tcatcatgaa ttcaaattca
     7321 tcgattaata tcaagaagat acaaaaagca ccatcattaa aattcattac agttacattg
     7381 atttcatcaa tgaaatgtaa aaatatataa acttgatgat ttaagcattt tcttataccc
     7441 gttcagacgt tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg
     7501 accggcagaa aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc
     7561 tctccacttc tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc
     7621 aactctcaaa tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg
     7681 tgttctgaac agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat
     7741 gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt
     7801 aaagccattg atgttcccgg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg
     7861 atggaagaat acgagcaacg ttcaagcacg ctggcacagt tagccgatga agcgaaagaa
     7921 ctgaatgatg attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat
     7981 ggcctgttac tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc tggtatgtgt
     8041 cctgtgcaga ccgaccaaca tgttctgaat gtcgtgtcgc atcagctgca ttgatcatac
     8101 atcggcgcta atgcattgcg ccgatgaagt ttttgagaaa ccgctgcctc atttttttga
     8161 agcagcggtt ttttaatggg attcgccctg tggggtaaac ttgagttcaa tcaacgcgat
     8221 ggctttttgg attgcccgca tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc
     8281 gatattcttc agctggctgg acattttttc gcgaacttca acgggcgcga tcacatcgag
     8341 aacatccaga atttgtttga taaccaactg gcaagcaacc acatcagaaa ccagttcctg
     8401 atcggcattc agcggctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag
     8461 tagcggcttc acatcttcag cgatagtcac atccaccgnn nnnnnnnntt gcaatagtta
     8521 ttgaacatac ttttcaggat cttgcgcagt ttcatcgcgg tactccgacc atttgttata
     8581 caggtgttat tgtctttgcg tttataatat gacaaccatc acaaaaatca atctttatgt
     8641 gatgtaaatc acataaacac ccctttaatg ttataaaaat gataatcaaa aagcagcccc
     8701 ccaatttctg acacatacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca
     8761 ttgtcgcagc gctgtaacgc gtaaaatact gctttctttt actcttctgg ctggaccatg
     8821 agacttctga ttctgactct ttccttaata acgctgacag gatgtacggt tactcgccag
     8881 gcccatgtga gcgaagttga cgcggcaaca ggtattgttc ggttggttta tgatcaggct
     8941 tttttgcagc atgcccgtac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc
     9001 cagcaggcag gctataccca cgctatcccc tttggtcagc cagtaggcaa ctgtagcctc
     9061 tttgcgggtt ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc
     9121 gccttccctg tctttctcta aacgttttaa tcagcgaggg ggatcttcgc tgattaaata
     9181 aatagcggta atgcgtttta ttcccattcg tatttttaat aaatgatgtt tatattttac
     9241 cttttgcaaa taataaaata acaaattata gtgacgccac agcaacaaat ataacctttg
     9301 tggagcacta tcatgctgaa accagaaatg attgaaaaac ttaatgagca gatgaacctg
     9361 gaactgtact cttcactgct ttatcagcaa atgagcgcct ggtgcagcta tcataccttc
     9421 gaaggtgctg ccgcgttcct gcgccgtcac gcccaggaag agatgacgca tatgcagcgt
     9481 ctgtttgatt acctgactga taccggcaac ttaccgcgta ttaataccgt tgaatctccg
     9541 tttgctgaat attcctcact tgatgaatta ttccaggaaa cctataaaca cgaacaatta
     9601 atcacgcaga aaattaacga
//
LOCUS       DAWDRC010000071         9548 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3614_length_9548_cov_2.464764, whole genome shotgun sequence.
ACCESSION   DAWDRC010000071 DAWDRC010000000
VERSION     DAWDRC010000071.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9548)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3614_length_9548_cov_2.464764"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1644..1653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3008..3017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4793..4802
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5015..5024
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6721..6730
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2162 a         2590 c         2325 g         2421 t
ORIGIN      
        1 taattaccga tctctctaac ccgcaaaccg ttttattttt tatcagtatt ttctcagtaa
       61 cattacatgc cgaaacacca acatgggcac gattaatggc ctgggcgggg attgtgctcg
      121 catcaattat ctggcgagtc tttcttagtc aggcgttttc tttgcccgca gtgcgtcgtg
      181 cttatgggcg tatgcaacgc gttgccagtc gggttattgg tgcaattatt ggtgtattcg
      241 cgctacgcct gatttacgaa ggggtgacgc agcggtgagc agataattta ctctctacgt
      301 aatcaacctg atttgccgga tcccgaccat cggcgacgat agcccccgta atgcgtggtc
      361 cttatttccg ataagtttac ttgagtcttt agcttcccat ttcagccact ctcccctgtg
      421 tgggcttttt cattggtctg acccgtcctg aatagccaaa caatggcaat gttccacttt
      481 gcagggatga taataagggg atctgattca gaaaagtttt gaaaagttgg accgccatcg
      541 aggcctcgaa ccccgtacca tacaacaatc ctggttgtcg tctgctcttc ctgatgagct
      601 aatggcggtt tttgatatga gccttacaac atattttctg tcaggcttga gtgcgatgcg
      661 taagatacag aaaagacccc acacaaacat gatggaatta tgattttttt catatgaatg
      721 tcaacccaca cattcacaaa acatctgtat gagaacattt tttatacacc atatagctgc
      781 gaaaccacta ctggtgcttt tttaagcgaa actccgccgt tcacaacaaa atatcggcga
      841 aaaaggaggg ggaatgccat ttggcataca gatatacaga tgaaatgatg acctgctttg
      901 cgattcactc tctggtggaa taacaacttg caggtggctc tcttaaataa aaataaacgt
      961 cgcagataat aaagctctga taaaaccaga cactatcaga aatcgctcat aactaacgtg
     1021 taaagtagtg aagttaattt aacaatctga atttacgcta taggtttacc actactttga
     1081 tattatttcg ctctccttaa ctaaaaagtt aagtggagcg aagttaatat gttaaaccac
     1141 aggatgttta ttaatttttt cagtctgtta gtgtgcgatt atcgataaca aaacgatatt
     1201 tcacatcacc acgcagcatt cgctcatagg cttcattaat ttgatcggcc cgaatcatct
     1261 ctatatcagc cacgatgcca tgttcggcgc aaaaatcgag catctcctga gtttctggaa
     1321 tgccgccaat catagaacca gctatcgcgc ggcgtttcat gatcaggttg aaaacttccg
     1381 gcgatttatg cggtgtcgca ggcgcaccaa ccagcgttat ggtgccatcg cgcttcagca
     1441 aggtggtaaa atcgtcgaga ttatgtggcg cagctactgt attcaaaatg aaatcgaaac
     1501 tcttcacatg agccaccatc tcatcggcat tgcgtgagtt aacaacttca tcggccccca
     1561 gggcttttgc cgcttcgcgt tttgactcag aagtggtaaa tgccaccaca tgcgcccccc
     1621 atcgcgtggg ccagtttaat cccnnnnnnn nnntatgtcc cagaccgccg atgccgacca
     1681 cgcccacttt tttacccggc ccggcctgcc agtggcgtag cggcgagtac gtggtgattc
     1741 ctgcacacaa taaaggagcc accgccgcca gctgctcttg cgggtgacga atacgcagaa
     1801 catatcgctc atgaacgacg atctgttgtg agtagccgcc cagagtatgg cccggttcat
     1861 ccggtgtcgg cgagttatag gttccggtca tgtgatcaca gtagttttcc agcccgtctt
     1921 cacactcttc gcaatgttta caactgtcga caatgcagcc gacaccaacc agatcgcccg
     1981 gcgcatgttt ttctacctga tcaccaacgg ctaccacgcg ccccacaatt tcatgacccg
     2041 gcacgcaggg gtaaaccgtc cccgcccact ccgaacggac ctggtggaga tcggaatggc
     2101 agacgccaca gtaagcgatt tcgattttga catcatgcgg tcccggttca cgccgggtga
     2161 tatccatcgg ttcaagcggt tgtttagcgg aatatgcacc aacagctttg atcttcattg
     2221 tgtttactcc tgattagcta tgtgtatttg gcgatgcctg atatgtcatc gcggcaaaac
     2281 gcgtccattg aatatagcca atatttaaac cacccgatcc agccccaaca tgccgcgagc
     2341 ctgaatgaaa cagagcagca acgttcccca taaggcgagc gcctataagt gtgatatttg
     2401 ctatccgtaa aagacaaaaa tcggcaaaac tgatgatatt ttcacgtaga caaatagtta
     2461 tctacacaac gtcaataagg gcatattctt caaagataat gtccatttct gccaacacac
     2521 tcatactcag gccgccccac tccctggggc ttaaatctct tactgttcac cgttgcaggc
     2581 gtttcaaatg cgcatcaacc tgccacactc ccccacacca gttgcccttt atggaacgtt
     2641 gcggttctcg gcgagatacg cgccaccgcc tcggcggaac aggaagcgtc caccagcaca
     2701 aaactggcgt catcctgcgc ttttggccac acgtgctcgc ctttttcatt tagcggcaat
     2761 acatcaccgg tggctaaaaa cagcgagcgg gataaattct gttcgttagg gcgaatatag
     2821 agctgcgcgt acagattggc tttttccagc atgtcgccca ggccgtaggg tgaccagtgg
     2881 tcgataacgc tgtcggtgcc ggtcatcact tttacgcctt tgtcgtgcag ctgtttgagc
     2941 ggcatatgca acgtgccaat cggcaccgtc gaggcgatag aaatttgctg cgccaccatc
     3001 cggttcgnnn nnnnnnnccg tgctgcggaa aggcgacgat ttcacactta aagcccgcct
     3061 gacgtcgcgc cagcaccgcc tgcaaatttt gcagattttt caggccggaa accggttcga
     3121 tattgcagtg gctgcgggca atggtggtgc ctttcgactg caataaatcg atcagttttt
     3181 ccgcccgttc ctgcgtgtac ggttgcagtt ccggcagcat tttctgctcg agtttgatca
     3241 tgtcctggat agtggtgcct gccggacgat tgagcgagcg ccacggcccg ccgtagaagg
     3301 ttttgtcgag atgaatatgc atgtcgcggg tggtgggcag catcagctta ccgccagcgt
     3361 catagtgcgg cgccgtgcgg gtctgtaccg ccacgccatt ttcgtagtca aaaccggttt
     3421 ccagcagcac gttatcgaga tagtaatgcg ggtcagtgat tttcatggtg ttgttcgctt
     3481 cgcagtttag ggtgccagct ccccccgcat gggcgagcgg cactgaggga ccaaacagcg
     3541 cggcggcggt aaccatctta ccgctctggc tcagaaattc acggcggcta ttgttttctt
     3601 tcatcttacg tccttaggtt ttaataatat gagtgcccgt ttcgccgcgt agcgccgcag
     3661 gcaggcattc cggcgtggtg ataatcactt ctttgccgcc ttgttccagg aacgtcaggc
     3721 tggcgatgat ttttggcaac atgctgccag gtgggaaatg cccttcctgc atatagcggg
     3781 tcatggtggc aatatctacg cgatccagcg cctgctgctg cggtttgcca aagtgaatac
     3841 acactttttc gacgccagtg gtgatcacca gaatgtcggc gtgaatttcg cgagccagta
     3901 gcgcggtaga gagatctttg tcgataaccg cgtccacgct ttggtaatcc cccgcttcgg
     3961 tgcgcaccac aggaattcca ccgccgcccg cgccaatcac cacaaagcct tgttggatca
     4021 gcgctttaat ggcaggtgct tcgacaatac gtttcggttc cggcgaggcg accacgcggc
     4081 gatagccccg tccggcatct tcaacaaaac gccagtcagg gtttgccttt tgtagttcgt
     4141 cacgctggct ttcactaaag aattcgccaa tgggtttgat gggatgggca aaccccggat
     4201 cgtttttatc cacttccacc tgggtcacca cggtgacggc ttttttctcg ccgtgacgcg
     4261 ccagtcggtt gttcagcgcc tgctggatca gatagccaat gccaccttgc gtatccgcca
     4321 cacagttcgc cagcggcgtt aagggcagcc cttcgcgctc gtgggcaatc tccgcgcggc
     4381 gtaaatccag cccgacctgc ggcccgttgc cgtgggtcag cacaatgtcg taatcggaag
     4441 ccagcatttc cagcaccgta tcggccacgg ctttcaccgc ctcagcctga tgctcaatcg
     4501 actggctggc gttatctttg ataatgctgt tgccaccaat ggcaacgacc acaagttctt
     4561 tcatgttgtt atgtccgcga cagagggtga tggggttatg accaacgcca gactgcctga
     4621 tgcgctacgc ttatcaggcc tacagattcc cgtgccattc gtaggccgga taaggcgttc
     4681 acgccgcatc cggctgtggt ggccaatgcc tgaagcgcca cgcttatcag gcctacagat
     4741 ttccgcgcca ttcgtaggcc ggataagaca ttcatgtcgc atccggcata aannnnnnnn
     4801 nngtaactca gacgccgtaa cgttcgcacc agccgagaat ggctttctcg aagcaggcga
     4861 agggtggatg cacaatgccc gcgccaatca tgccgacgcc cgcatcttta tgggcaatgg
     4921 cggtgttgat cactggcaga atgccactgc tacccacgcg ggtgatgtcg attgccgacg
     4981 gcacgcccat aaatccgagc agcggaatgg tgacnnnnnn nnnngttcgg gttttcgcca
     5041 agggtgattt cacgcatctg gcgggagaaa tcgatagcct cttccaccgt accgcccacc
     5101 agcgcgacga tagcgggtgc ggtcgccata gcaaatccgc caataccgta ggtttcagta
     5161 atggcgctgt cgccgatatc cagccccgaa tcttccggct tataaccggc aaacatcggg
     5221 ccgatgacct gttgcgccgg gccggtaaac cactgccccg gcaggccgct gactcgcagg
     5281 ccgaactcga cgccgttacg cgccatagtg gtcaccacgg tgctgtattc gatgccgtgc
     5341 gccgcatcca tcgccgcttt acacatcgcc atccacgtcg ggccggagaa gtagtcgctg
     5401 ctggcgacaa actcaaacac ttcgcgctgt tgctcgacgg aataacctgc ctgaataatc
     5461 cccggcgtca gcgcctgaat cagcaacgtc gtcccggcgt tattgcggtt atggcattca
     5521 tcgcccatat gcagcgcctg cgccagcatt aagcgcagat cgatttcgcc gataattttc
     5581 atcgcgtcgc gcagcatggg tccctgcaca tcgcgcatcc agttgaggcg gtcgatcacg
     5641 ctctggtcgt tagcgcccat acgcaaaatc ttcgccatct gctcgctcat gttggtataa
     5701 gcgatgttgc cataggtttt gtttttcacg atgtgcataa acatcgaggc cgaggtgacg
     5761 cccgccatcg atcccacaca gtcgtgttcg tggcacggcg agaaggtgat ctccccggaa
     5821 gccgccagct cagccgcctc gtcgagatct ttcgccagtc cttcaaacac cagcgctccg
     5881 gtgaccgcgc ctttcatcgc gccgcacatt ttttcccagg tgaccggcgg cccggcgtga
     5941 agaatggttt tcggcgtcat gcccggcacc acgttaatcg cccgatcaaa accaatcagc
     6001 atcggatgcg actggataat gcgctccagc gcctgctggt tagcggcggt gattttgtcc
     6061 gccagcggcg aatcggcaat gttgtccagt gcctgcacca cctgcatatt gccctgcccc
     6121 ggcggcgtcc agtcgagttg ggtcacttct acatgctgct ttttcagatc atcgctaaac
     6181 atggcgatac cgacgttaat aacgttcaac ggttggctaa acagtgactg gctcatcagg
     6241 cttcctcccc tttgcagata aattcacgcg ccagcaatcc ggtattggtg ctgctgctcg
     6301 ccagaatcac tccggcatca agcagcatct ggctttgttg ttctaatgat ggcgtatcga
     6361 gatcggtgcc cagcacatag gcgagaatga tcaactcgcg tccttcggca gcggcgatcg
     6421 ctttcgcttc tttgatcgcc tcgatggtgg aaccgaccgg atcttcatgc gatccaaatc
     6481 cgagcacaaa atccatcacg atcaccgcca cttctggatc gcgcgcttct tcgatcaagc
     6541 ggctgatacg gttggtcggg tcaatcattg gatgcggctt gccgttggtg aagtcatcat
     6601 cgccaaaatc gaggaaggtg tgtttgatgc tgcggttgat atcttgcagg cggaattcag
     6661 gatcgggctg gatattgctg tagacatcgc catgtttttc catcaccgcg aacagggttt
     6721 nnnnnnnnnn atgcaggtcg agatgttctt gtttcacgcc ggagagcatc accgctttca
     6781 atgccgcctc tttgctgccg cgagcaaact gtagcccctg ctcatccact ggcgtttcgc
     6841 cacgaccgag gaagcaggcc accactggct tgcggcaggc gcgtgcgcgt tccagcactt
     6901 tgcgggccac cgcaggcgca ggcggtttgg agataagcac aatgatttca gtttgcggat
     6961 cgttttccag catcccgatg gcatcgagca tcatcaggcc accgattttc tcgctcaggt
     7021 cgcgcccgcc ggtgccgatc agttgtgaaa cgccgccacc aaattcatgg atacggacgc
     7081 tcaattcctg gctgccggtg ccagatgcgc caacaatacc gatgttgccg cgacgcacgg
     7141 cgttaccaaa gcagagcgcc gcgccgttga taatcgccgt gccgcagtct ggccccatca
     7201 tcagcagccc tttttcatgg gccaactgtt tgagcgccag ttcatcttca actgagacat
     7261 tatcggaaaa cagcatcacg ttgaggtcat tttgcagcga ctgacgcgct tcgcgagcgg
     7321 caaacagacc gttgaccgaa atcactgcca ggttactttc cgggatatgc tttttggcgc
     7381 tggcaatagt ggcgtaacgc gcctcgtgcg agccgctttg cgctttggtg ttgaacagtt
     7441 cttcaatctc cgccagcaac tgctcgttgt ccacacccga tttaccattg atgacaatca
     7501 tcaggtcgcc gtttttcgct tgctccagct ccggcgtcag cagtcctaaa ttcttcaaca
     7561 cgcctttgtt catttcggtc gccatcgcca caaatgcctg ctcgacgccg tcgagtttat
     7621 ttgcacgcgt ggagatagac atcagcgaga cagaatcaaa ataggtattc ggtttaatga
     7681 ctattttaac tgacataatt ccttccgtag gtttggctca gcgcacaacc atcggccatg
     7741 ccatacagca tgtcgcagcc agaactggag ccaatatgtt taattttttc tattacctgg
     7801 gttgaattcc cagggacacc ataaataata tcgtgaataa aatgatgaat attttcccgg
     7861 ctgcgttgtt gtaatgcggc ttccagcgtt atggcactta ataacgtggt attatttctg
     7921 ccgcgttgca gaccgcgata aaattcctct ttgtattttt ctgccggatg cccggaaata
     7981 aataaaataa gtaccagtcc ggtcagataa tcatcggctg atggcgtaag gccaatccct
     8041 aagcccatca tactggcgac tgctgaggcg atattttgtt tatcgtttac ggcctgaata
     8101 actgcccgtc gtttaatatg tagttcttta tttaacgcct gataaaacgg attatcgccg
     8161 cgataattaa acagggtttc attttccccc aggtgatgat gaataatgtc gtgccactgt
     8221 tgccaggcga taaggtgaaa attcacagcg gtcatttgca aggttgggga cagccaacgc
     8281 tggcaatgac tcatctctat atgaagatgt tgaccaaccg taatgccttg atcgtcaaac
     8341 tgaaccttat caccatgccg gaacagacca ttaaagtgag tgagtgccaa ccgacaactg
     8401 tttggcgcat tgtcgtactc ttcacaaagc agcgtcagca actgctggcg agccgggagt
     8461 gcgatattta ccgctctggc aaagacctgt tcaacctgtc cttgcccccg ctgcgccaga
     8521 aaatcagcat ccgcagttag cgcccacaca caccgttttt ctggtgtgtt gataacaagc
     8581 ctcccggttt atgcacctgc ggcgatcagc aactgcgcaa tctcttcaaa gccccgttcc
     8641 cgcgccagtt ccagcggcgt tttgccatat ttatcggtca gatgcgggct ggcaccgtgt
     8701 tccagcaata actggacaat cgcctgctgt ttaatcccgc catcgttaag cacaatcgct
     8761 tccagcaacg gtgtccagcc aacatggtta gtctggttaa cgttaatctc cgtatttgcc
     8821 aaaagctctt ttacaatact taaatggcct ttttcacagg caggcgtcag gccgacaccg
     8881 ccaaaacggg ttacgcaatt aagatcgggt ttagccggta aaataattcg tagtagcgtt
     8941 aaatcatcgt tcagacagct aattaaaaaa ggatttaaac aggtatgatc ttgtttatta
     9001 atattcgctc tagcgtcaat taatgcctga acgcaagcat attgctgata taaacttgcc
     9061 agcgtaattg ccgttttccc ctgacgatcg caggtattaa tatcgacacc cagcgcaagg
     9121 caggttttta ctttatcgat atcaccctgt tgcgcagcca ataaatagtc tgcaggtaga
     9181 ttttttatag tcattcacat tcacctggtt tttgtggctg ttttcgatga attgagaata
     9241 gcaacgataa tctgataaaa gaacccgctt aaatttaatt cgccgtattt cgcctttttg
     9301 ttgaatgatg ttcaacaatc ttttcgcagt gtgcggtagt gcaatatttg tttctttgac
     9361 ccgataccct ttcactcaat caggttttat ttctctatag tctgcacatg taagagtagc
     9421 tttcgtccat gtctaaccag aggggaacaa gatgaacgga ctcacggcaa cgggtgtcac
     9481 cgtcggtatt tgtgcagggc tttggcagtt agtgtcttca catgtcgggc tctctaatgg
     9541 atggggat
//
LOCUS       DAWDRC010000072         9509 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3632_length_9509_cov_2.986461, whole genome shotgun sequence.
ACCESSION   DAWDRC010000072 DAWDRC010000000
VERSION     DAWDRC010000072.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3632_length_9509_cov_2.986461"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2465 a         2498 c         2196 g         2350 t
ORIGIN      
        1 accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc ccgcgcctga
       61 aacgtggcaa attctactcg ttttgggtaa aaaatgcaaa tactgctggg atttggtgta
      121 ccgagacggg acgtaaaatc tgcaggcatt atagtgatcc acgccacatt ttgtcaacgt
      181 ttattgctaa tcacgtgaat gaatatccag ttcactttca tttgttgaat acttttacct
      241 tctcctgctt tcccttaagc gcattatttt acaaaaaaca cactaaactc ttcctgtctc
      301 cgataaaaga tgattaaatg aaaactcatt tattttgcat aaaaattcag tgagagcaga
      361 aatccaggct catcatcagt taattaagca gggtgttatt ttatgacgac gattctcaag
      421 catctcccgg taggtcaacg tattggtatc gctttttccg gcggtctgga caccagtgcc
      481 gcactgctgt ggatgcgaca aaagggagct gttccttatg catatactgc aaacctgggc
      541 cagccagacg aagaggatta tgatgcgatc cctcgtcgtg ccatggaata cggcgcggag
      601 aacgcacgtc tgatcgactg ccgcaaacaa ctggtggccg aaggtattgc cgctattcag
      661 tgtggcgcat ttcataacac cactggtgga ctgacctatt tcaacacgac gccgctgggc
      721 cgcgccgtga ccggcaccat gctggttgct gctatgaaag aagatggcgt gaatatctgg
      781 ggtgacggca gcacctataa aggaaacgat atcgaacgtt tctaccgtta cggtctgctg
      841 accaatgctg aactgcagat ttacaaaccg tggcttgata ctgactttat tgatgaactg
      901 ggtggccgtc atgagatgtc tgaatttatg attgcctgcg gtttcgacta caaaatgtct
      961 gtcgaaaaag cttactccac ggactccaac atgcttggtg caacgcatga agcgaaggat
     1021 ctggaatacc tcaactccag cgtcaaaatc gtcaacccaa ttatgggcgt gaagttttgg
     1081 gatgagagcg tgaaaatccc ggcagaagaa gtcacagtac gctttgagca aggtcatccg
     1141 gtggcgctga acggtaaaac ctttagcgac gacgtagaaa tgatgctgga agctaaccgc
     1201 atcggcggtc gtcacggcct gggcatgagc gaccagattg aaaaccgtat catcgaagca
     1261 aaaagccgcg gcatttatga agcccctggg atggcactgc tgcacattgc gtatgaacgt
     1321 ttgttgaccg gtattcacaa cgaagacacc attgagcagt atcacgcgca tggtcgtcag
     1381 ttgggccgcc tgctgtacca gggacgttgg tttgattccc aggcgctgat gctgcgtgac
     1441 tctctgcaac gctgggttgc cagccagatc actggtgaag ttactctgga gctgcgccgc
     1501 gggaacgatt attcaatcct gaataccgtc tcagagaacc tgacctacaa gccagagcgt
     1561 ctgacgatgg aaaaaggcga ctcggtgttc tcgccagatg atcgtatcgg tcaactgacc
     1621 atgcgtaacc tggatatcac cgatacccgc gagaaacttt tcggttatgc caaaactggc
     1681 ctgctctcct cctctgccgc ttcaggggtg ccgcaggtgg agaatctgga aaataaaggc
     1741 cagtaattcg acatcaaccc tgcccttcag gggtggggtt accttgatta tcctctgtaa
     1801 actcctttct gagcatcaca taatcgaaaa catcatggtt tgcatccaca acccgtgagt
     1861 ctcctttata gtgtgcaatc acttcctcaa gcgtttgcgg ctgttccggc tttgttaccc
     1921 ccatccacgc attaataagg taattattgt aagctgtcga aaagatgttg ttttccgttg
     1981 tgcgatccac gccatcccct agaacggggc tataccagat aaacatcggg acatgatacg
     2041 cctgctggct agcctccctg cctccatgaa aatagacatt cttcttcgta gggtcgcgtt
     2101 ccaggccgtg gtcggcaaaa tacatgaccg aggcgcggcg atcttttaat aattcaaaaa
     2161 cctgacctag caaactatct gtgtaatgaa tggagttgtc atagcaggca tcctgatcgt
     2221 cctgcggttg aaacacggcg ctggattgcg gataggcgct acaagccggt tcatggcttc
     2281 catttaaatg aagaacaatc agtttttttt gctgcgtctt ttgctgtaac gcttgcgata
     2341 aatgcggcaa caacaattca tcaaatcctc tgacatagac tgtttccatg gcgcgcatgg
     2401 cgatactagt aactgctgta ccattctgcc ggaaagcgga ttgcgagctt agccagaaag
     2461 tctgaaatcc tgcttgatta gccatattaa taatgttgtc cggataatta tgaatgtcat
     2521 gactcaaaac agaatcagca gttaaagaaa ggggaaccga cagcgcagtg taaggtgcac
     2581 cgcttattgc ttgattaaac agtttgatct gctttctttg tgcttcgact tgcggtgtcg
     2641 tagagcgtgt atatccatac aaagacatat tgtcgacacg tacagactcc cccacaatca
     2701 acacgtaggt atcaatacct gtatccctga ctgatagttg aaaatacggc accgtgtttg
     2761 caattgagag taatctttga tgctctttcg ctgctaaagc aaaataattg agattgaaaa
     2821 acggcgtata ggtagcaaat cgcgacgcta gtatatatgg actgaacgcc tctttatttt
     2881 ttgcatcttt ataagcaaat tgacaagcgg aaaataaact gcccgaaatg acaatcagca
     2941 ataatattcc tgtcactttt tttgtcggca aggaaacatc atattttatt attactgcaa
     3001 aaaaaagaag ggataaaaag gcaaaagcac atagataagg gatatacatt cccagcattt
     3061 tgacaacttc atccggatca ctctgcagca cactaatcgc aaatccatca ttgaaagttg
     3121 taccaaagat acaccatgaa tagaggctaa tactcatatc tgccgcagtc agtaacacaa
     3181 atggtacggc aataagcagg cgaagcggaa gggatgatat acgtttaaca accaggatgg
     3241 tcagaacgaa aaacagcaat cgctgaacca tatgcgctcc ggaagaggcg actaagttcg
     3301 taataccaaa aacaataaca caaagataca acaaccaatg agatttaaaa cttctaacaa
     3361 atttgttgaa tactgtcatc cagaaatcca agttcacaac caatataaac aattatcaac
     3421 aatgttcaat tttatatcag acatgttttt caactctgat ccatatcaga ccgaaacact
     3481 gttagttttt tctttttaaa tgatgcggcc attgttctca gtaattaaat aacattatca
     3541 atgcgtctct aaacattacg cagaacgtag ccatcagtag ttatactgac aggagcagga
     3601 tggtttatcg agaagtaaac atatccgtat tcgtaccgga actggagttg acacaataaa
     3661 gtgccaatta tgtcagcaga agggaaaagt ttataactaa ggcgtaccgg caccatcgtt
     3721 tcaaagtacc agctacgagt aaagcaactg gacgagatac agatacctga caaccattcc
     3781 tcagaccagg accaaaacga aaaaagacgc ttttcagcgt ctttctggaa tttggtaccg
     3841 aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta
     3901 ccaattccac cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt
     3961 agccggagca gctggctgag tttgttcggt tttcgccggc gcactcaggt tttcccattc
     4021 gctaccttta ttggttttgt tgctattgat gttacccagc accagactga tgatgaagaa
     4081 taacgttgcc agcagcgctg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa
     4141 cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg
     4201 cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacta aaagagcttc
     4261 gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc
     4321 gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaagc gaatgacctt
     4381 cgcaagggca attttattgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt
     4441 tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca gttatctaac gctttaaacg
     4501 gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct
     4561 tccaccatca cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg
     4621 ctacccagcg ccgcttcaac ttctgcggtc acagctttga ctgattcatg ctccagcgga
     4681 tcgccgctac ctgcggtgta acgcacgttt accaggattt gcgggaacat tttcatgccg
     4741 ctgcaaaggt cgtgcaggct catatgatta cgcgccatcg ccgccagcac ctgcaaacca
     4801 gcaacgatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct
     4861 gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc
     4921 gcgcgcgcaa atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc
     4981 aatgtaccca cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata
     5041 tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg
     5101 ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc
     5161 gcgcgaacgt cggtagcccc cacttcggca ttgatgttta caccgttcgg ctcacaaccg
     5221 atagcgataa cgttcgctcc cagttcacgc agcacgttcg gcgcgatgtg ataagtcgca
     5281 ccgtttgcac aatccaccac aatcttcagt tcactgaggc taagttcgtt tgggaacgtg
     5341 gctttgcaaa actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt
     5401 tctgccgaat caacacagct gatctctttt tccatttccg cttcgatggc ctcttctact
     5461 gcatccggta gtttggtgcc gtcgatagag aaaaatttaa tgccattatc gtagaacggg
     5521 ttatgcgatg cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc
     5581 acggccggtg ttggcatcgg accagtgaag agtgcggaaa ggcccgcagc cgccagaccc
     5641 gcttccagtg ctgactccag catgtagcca gaaatacgcg tgtctttacc aatgatgatc
     5701 ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa
     5761 tcaggggtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta
     5821 cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc
     5881 ttctacggtt tctttgacgt catgaacacg aatgatatgc gcgccttgca tagcggcaat
     5941 gaccgcacaa gccagactac cgctcagacg ctcggacggc cccacgttca gcagctgccc
     6001 aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc
     6061 caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc
     6121 gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa
     6181 atagcgattc acttctgcaa agacatcgtc atacttcggg gcttcctgca tggtctttgg
     6241 atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagctg cctccagagc
     6301 ccctggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc
     6361 acggatgact tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat
     6421 tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgctcc
     6481 tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt
     6541 cgcatgtttc acggcatcta tcagcgagtt atgcgtgccg ccatccgaaa aggaatcagg
     6601 cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc
     6661 aaagagtttc atgatgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc
     6721 ccggggtttt cggtacaaat acagtcatct gatacgggaa cttacttgtc gcctaactgc
     6781 tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt
     6841 ggactaccat tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga
     6901 cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc
     6961 atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag ctggcgcgca
     7021 cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca
     7081 tcggacatat gtttcgcttt cgctacgcta cgaccgagga acacttcacc ttcttcttcc
     7141 gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat gttacgtgcc
     7201 aggttggtcg caactttaat gtcgttggac gccccggtag aaacatgttc cggtccgtag
     7261 atgatctctt ccgccagacg accaccgtac agcgtggaga tctgactttc cagtttctga
     7321 cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg
     7381 cgtgggataa tcgttacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc
     7441 gcgtggccag cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag
     7501 cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac catcgaaaca
     7561 acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca
     7621 gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat
     7681 ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagcca
     7741 accacaacct gacggtcgaa acggccagga cgcagcaggg ctgggtcgag aacgtccgga
     7801 cggttagtcg cagcgataac gatgatacct tcgttacctt caaaaccatc catctcaacc
     7861 agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccaagcc tgcgccgcgc
     7921 tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggcgccgc tttcttcgcc
     7981 tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca
     8041 gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caattgcttt cgccagcaac
     8101 gttttaccgg tacccggagg acctaccatc aggacgcctt tcgggatctt accgccgagt
     8161 ttctggaagc ggctcggctc gcgcaaatac tcaaccagtt cagcaacttc ttcttttgct
     8221 tcgtcgcagc ccgcaacgtc agcaaaggtg gttttgatct gatcttccgt cagcatgcgc
     8281 gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg
     8341 aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc
     8401 agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta
     8461 tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg
     8521 gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct
     8581 tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg
     8641 aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca
     8701 ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc
     8761 cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca
     8821 atatacactt cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta
     8881 aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact
     8941 acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga
     9001 tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac
     9061 atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca
     9121 tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg
     9181 caagcgatga tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc
     9241 ggagctgcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt
     9301 atttcatcaa gtttaaacca ggcacgggaa cgtaaccccc ttttctgtgc ctgttgaaca
     9361 tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct
     9421 gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc
     9481 ctatttggtg atatatggga gatggcggt
//
LOCUS       DAWDRC010000073         9476 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3647_length_9476_cov_2.265046, whole genome shotgun sequence.
ACCESSION   DAWDRC010000073 DAWDRC010000000
VERSION     DAWDRC010000073.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3647_length_9476_cov_2.265046"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    222..231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3369..3378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5636..5645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8338..8347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2253 a         2257 c         2460 g         2466 t
ORIGIN      
        1 tggttaacgg tctggcgtat gtcatgatca ccgaaaacct ggtagatcag gaattcctcg
       61 ataaatattg cgttggctac gacgagaaaa ccctgccagc cagtgcgccg aaaaatggtc
      121 actataaagc ttatattctg ggtgaaggac ccgatggcgt ggctaaaaca ccggaatggg
      181 cctcgcaaat caccggtgtt ccggcagaca aaatcatcaa annnnnnnnn ncggtgaaat
      241 cgcaacccgt gctatctcga tgctggcgat tctgaccggt aacgttggta ttaacggggg
      301 gaacagcggc gcgcgcgaag gttcatacag cttaccgttt gtccgtatgc cgactctcga
      361 aaacccgatc cagaccagca tttctatgtt tatgtggacc gatgccattg aacgtggccc
      421 ggaaatgacg gcgctgcgtg atggtgtgcg cgggaaagat aagctggatg tgccgatcaa
      481 aatgatctgg aactatgccg gtaactgcct gattaatcag cattctgaaa tcaaccgtac
      541 ccatgaaatc cttcaggatg ataagaagtg cgagctgatt gtggttatcg actgccacat
      601 gacctcatcg gcgaaatatg ctgacatcct gctgcctgac tgcaccgctt ccgaacagat
      661 ggactttgca ctggatgcat cctgcgggaa tatgtcttac gttattttca atgatcaggt
      721 cattaaaccg cgctttgaat gtaagactat ctatgagatg accagcgaac tggcaaaacg
      781 tcttggcgtc gaacaacagt ttactgaagg tcgtactcag gaagagtgga tgcggcatct
      841 gtatgcccag tcgcgggaag cgattcctga actgccaacg tttgaagagt tccgtaagca
      901 ggggatcttt aaaaagcgcg atccgcaagg gcatcacgtt gcttacaaag ccttccgtga
      961 agatccgcag gctaatccat tgaccacgcc atcgggcaaa attgagatct attcacaggc
     1021 gctggctgac attgccgcta cctgggaatt gccagaaggt gatgtgatcg atccactacc
     1081 gatctacacg ccgggctttg aaagttatca ggatccgctg aacaaacagt atccgctgca
     1141 gcttacgggt ttccactata aatctcgcgt tcactcaact tacggcaacg ttgatgtgct
     1201 gaaagcagct tgccgtcagg aaatgtggat caacccgctt gatgcgcaaa aacgcggtat
     1261 caacaacggc gataaagtca ggatctttaa cgatcgtggt gaggttcata ttgaggcgaa
     1321 agtgacgcca cgaatgatgc cgggtgtggt cgcactgggc gaaggtgcct ggtatgaccc
     1381 ggatacaaaa cgtgtcgata agggcggttg tatcaacgta ctgaccactc aacgtccgtc
     1441 tcctctcgct aaggggaatc cgtcacatac aaacctggtt caggttgaaa aggtgtaagg
     1501 agtaaccgat gacaacccag tatggatttt ttattgattc cagccgttgc accggttgca
     1561 aaacctgcga gctggcctgt aaagactaca aagatttgac gccggaagtc agcttccgcc
     1621 gcatttatga atatgctggc ggtgactggc aggaagataa cggtgtctgg catcagaacg
     1681 tgtttgccta ctatctgtcg atttcatgta accactgcga agatccggct tgtactaaag
     1741 tctgcccgag tggtgcgatg cataaacgtg aagatggttt tgttgtggtc gatgaagatg
     1801 tgtgcattgg ctgccgctac tgccatatgg cttgcccgta tggcgcaccg caatataacg
     1861 aaacgaaagg ccatatgacc aaatgcgatg gttgttatga ccgtgttgcg gaggggaaaa
     1921 agccgatctg tgttgaatct tgcccgctgc gggcgctgga tttcggccct atcgacgagc
     1981 tgcgtaaaaa acatggcgat ctggcggccg ttgcgccgtt gccgcgagct cactttacca
     2041 aaccgaatat tgtgatcaaa cccaatgcca acagccgccc aaccggggat accactggct
     2101 atctggcaaa cccgaaggag gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg
     2161 atcttcacgg tcttcgggca atgtgtagcg ggtggtttta tcgttctggc tttggcgctg
     2221 ctcaaaggcg acctgcgagc agaagcccag cagcgtgtta tcgcctgcat gttgggttta
     2281 tgggtgctga tgggcattgg ctttatcgcc tctatgcttc atcttggttc accgatgcgc
     2341 gcttttaact cgctcaatcg ggtaggggct tcagcactca gtaacgaaat cgccagcggt
     2401 tcgatctttt ttgctgtagg cggcatcggc tggttgctgg caatgctgaa aaagctttca
     2461 ccggcattgc gtacgctttg gctggtggtg acgatggttc ttggcgtagt ctttgtctgg
     2521 atgatggtgc gtgtgtataa cagcatcgat acagtaccca cctggtacag catctggaca
     2581 ccgatgggct tcttcctgac gatgtttatg ggcggcccgt tactgggtta cctgttgctg
     2641 agtctggctg gtgtcgatgg ctgggcgatg cgtctgctgc cagccatttc tgttctggca
     2701 ctggtagtga gtggcgtgat gtcagtgatg cagggcgcag agctggcgac tattcatagc
     2761 tctgtgcagc aggccgcagc gctggtgccg gactatggtg cgctgatgtc ctggcggatc
     2821 gtgcttttgg ccgttgccct gtgcttgtgg attgcaccac agctaaaagg ttatcagcct
     2881 gcggttccgc tactttctgt atcattcatt ctgcttctgg caggggaatt aatcggtcgt
     2941 ggcgtattct atggtttgca tatgaccgtg gggatggctg tcgcaagtta atcagtaaca
     3001 accggggttt cggccccggt tcgcttttat ttctgcttcg ttaacgtgtc ataactggtc
     3061 atcaagttac gataatcagg aatgtggtta gagaacaacg tcgccagtcc ttcaatatca
     3121 ttacgccagt cgcgatgcag ctcgcatgcc acgccgaacc atgtcatcaa ttgcgcacca
     3181 gcttgcgaca tgcgatccca tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg
     3241 tcagtcacga caaagacgtc aaacccttct tcaatggctg aaagcgccgg gaatgccacg
     3301 caaacttcgg ttaccacacc ggcaataatt aactgttttt tacctgtcgc tttgacagct
     3361 tttacaaann nnnnnnnnta atatttcccg ggcgagcaat gtaaggtgca tcggggaatt
     3421 gtgctttcag ttctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa
     3481 tagttggtaa gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact
     3541 tatcgggttc gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa
     3601 cggcagcatc atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag
     3661 agtcagtggt acgtctaaat cggtcagacg cgatgcattg ctctgaaagc atagacggga
     3721 aatatgagtt tgctgtgacc atgaaatttt tcgactgaac gaagcgtatg aaatttgtgt
     3781 tagatcaata aaaacaatca gatgagatta atcgtctctt cagcattagc gttatgaata
     3841 agtaatataa atcgataata ccgccgctgc gatatgcgcg tattttcacc cattgacaat
     3901 gtttttggcg gtggcatgat gcgcatgaaa tttgaacttc ctcacggttt taattcatgt
     3961 ccacgtatac ccggcctgtc atgcttttgc tgtctggcct gcttttgttg actctggcga
     4021 ttgcggtgtt aaatacactc gtgccgcttt ggctcgccca ggaacagatg tccacatggc
     4081 aggtaggcgt tgtcagctca tcctatttta ccggcaacct tgtcggtaca ttgctgacag
     4141 ggtatgtcat taaacgcatt ggctttaacc gtagctatta tctggcctcc ttcatttttg
     4201 ccgctggctg tgccggcctt ggcctgatga ttggtttctg gagttggttg gcgtggcgtt
     4261 ttgtcgcggg cgtcggctgt gccatgattt gggtagtcgt tgagagcgcg ctgatgtgca
     4321 gtgggacgtc acgtaaccgt gggcgtttgc ttgctgcata catgatggtt tattacgtgg
     4381 gaacgttttt aggccagtta ctggtcagca aagtctcaac cgagttgatg agtgtattgc
     4441 cgtgggttac agctttgacg ttggcaggaa tcttaccgct gttgtttacg cgtgtcctga
     4501 atcagcaggc tgaaaaccat gattcgacgt caattacgtc aatgctaaaa ctccgtcagg
     4561 cgcggcttgg cgtgaatggc tgcattatct caggaatcgt gctgggatct ctatatggcc
     4621 tgatgccgct gtacctcaat cacaaagggg tgagcaatgc cagcattggt ttctggatgg
     4681 cggtactggt cagtgcgggt atcctcgggc aatggccgat tggacgtctg gcggataagt
     4741 ttggtcgact gctggtgttg cgtgttcagg tctttgtcgt cattctcggc agtattgcga
     4801 tgcttagcca ggcggcgatg gccccagcgt tattcatcct cggtgccgct ggctttacgc
     4861 tatatccggt ggcgatggcc tgggcttgtg agaaagttga acatcatcaa ctggtggcga
     4921 tgaaccaggc cttactgttg agctataccg tgggaagcct gcttggcccg tcatttaccg
     4981 ctatgctaat gcagaatttc tccgataatt tattatttat catgatcgcc agcgtatcgt
     5041 ttatctattt gctgatgctg cttcgtaacg ccggtcatac gccgaaaccc gtggctcacg
     5101 tgtaaatgaa ttcaagcaga gtgtgaactt actgtttcac actctgcttt tctgtgcaca
     5161 aaaaagcggc aacttaataa agttgccgct ttacggggaa attagaacat gaccttatga
     5221 ccgtactgct caagaatgcc tttcacgcgt tccatggtct ctttcttcgg tggtttaaca
     5281 ccatcgagtt tgtattcttc acccattgcc acccatttgt gtttgcccag ttcgtggtag
     5341 gggaggagct cgattttctc aacgttgccc atatcacggg taaattcacc aaggcgatgc
     5401 gctgaatcgt catcgtcaga ccagcctggg acaacaacat agcggatcca caccttcaca
     5461 tttttgttcg ccagatattt agcgaactcc agcgtgcggt ggttggaaac tccaaccaga
     5521 ttttggtgga tctcgtcgtt catctgtttg agatcgagca ttaccaggtc ggttacttcc
     5581 agcagttcat caatcaccgg atcgtaacga cgaacaaaac cgttggtgtc cagacnnnnn
     5641 nnnnnccttc ttttttgcag gcgcggaacc agtcacgaac aaactcagct tgtaggattg
     5701 cctcaccgcc ggatgcggta acgccgccgc cggaagcgtt cataaagtgg cgataggtca
     5761 ccacttcctt catcaaatct tcaacggtaa cttctttacc gccatgcgta tcccaggtgt
     5821 cgcggttatg acaatacagg cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa
     5881 tacccgggcc gtctacggtt ccacaggatt caaaggagtg aatgcgacca ataactgaca
     5941 ttgcggtgtt tctccagatg tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct
     6001 acgtcgagtc tgttttggca gtcaccttaa agtatagata gctgacaaaa aaggctctcg
     6061 tgctaaaaaa ggccccactt tcgtggagcc tttattgtac gctttttact gtacgatttc
     6121 agtcaaatct aattacatag attgagtgaa ggtacgagta ataacgtcct gctgctgttc
     6181 tttagtcagc gagttgaaac gtactgcgta gccagataca cggatggtca gctgcggata
     6241 tttttccggg ttttccatcg cgtcgagcag catttcacgg ttcatcacgt taacgttcag
     6301 gtgctgacca ccttcgatgg atgcttcgtg gtggaagtaa ccatccatca gaccagccag
     6361 gttggtctta cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag agaaggtgta
     6421 ggagatacca tctttagcgt aagcaaacgg cagtttagca acggaagtca gagacgctac
     6481 agcacctttc tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc
     6541 acgacgaccg tctggggtgt taccagtttt cttaccatac acaacgttag aagtgatggt
     6601 cagaacagac tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat
     6661 gaaacgttct accaggtcaa cagccaggtc atctacacgc ggatcgttgt taccaaactg
     6721 cgggtattcg ccttcgattt cgaagtcgat agccagaccg tcttcgtcac gaatcggttt
     6781 aactttcgca tatttgattg cagacaggga gtcagcagca acggacagac cagcgatacc
     6841 acacgccatg gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct
     6901 gtacttgtcg tgcatgtagt ggatgatgtt cagtgcagtg atgtactgtt tagctagcca
     6961 gtccatgaag tgatccatgc gctccatcac ttcatcatag ttcaggacat cgcctttgat
     7021 cggttcagac ttcggaccaa cctgcatttt cagtttttcg tcaacgccgc cgttgattgc
     7081 gtacagcatg gttttcgcca ggtttgcacg cgcaccgaag aactgcattt gtttaccaac
     7141 gatcatcggg cttacgcagc aagcaatagc gtagtcatcg ttgttgaagt ccggacgcat
     7201 caggtcatcg ttctcatact gcagagaaga ggtgtcgatg gacactttag cggcgaattt
     7261 cttgaagttc agcggcagtt tttcagacca cagaatggtc atgttcggtt ccggagacgg
     7321 acccatggtg tacagggtgt tcaggaaacg gaagctgttt ttggtaacca gggtacgacc
     7381 gtcgaggccc ataccaccga tagattcggt tgcccagatc gggtcgccag agaacagttc
     7441 atcgtattcc ggagtacgca ggaagcgaac catacgcagt ttcatgacca ggtggtcaac
     7501 catttcctgc gcttcttgtt cggtgatctt gccagctttc aggtcacgtt cgatgtacac
     7561 atccaggaag gtggaggtac gaccgaagga cattgcagca ccgttctgag acttaacagc
     7621 agccaggtag ccgaagtaag tccactggat agcttcctga gcgttggtag ccggaccaga
     7681 gatgtcgtag ccgtatttcg cagccatttc tttcatctga cccagagcgc ggtgctgttc
     7741 agcgatttct tcgcgcagac ggatagtctg ttccaggttt acgccgtttt ccagatcagc
     7801 ctgcagagag gtgaactgtg caagtttgtc tttcatcagg tagtcgatac cgtacagcgc
     7861 aacgcgacgg tagtcaccga tgatacgacc acggccataa gcatctggca gaccggtcag
     7921 aacaccggat ttacggcaac gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt
     7981 gtgagtttta cggtattcag tgaagttttt tttgatcatc gggtccagtt cgcggttgta
     8041 cgctttgcag gaaccttcga tcattttgat accaccgaac gggataagag cacgtttcag
     8101 cggagcttca gtctgcagac caacgatttt ctcaagctgc ttgttgatgt aaccagcgtc
     8161 gtgagaggtg atggtggaag ctacagcggt gtcaaagtca actggcgcgt gagtgcggtt
     8221 ttccagttta acgccttcca ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc
     8281 agccaggaag gactcgtcac cctcgtacgg agtgtagttt ttctgaatga agtcacgnnn
     8341 nnnnnnngtt tacttcattc tgccagtcac ctttggtaaa accttcccag gctgtggcta
     8401 acttttcatt aagctcggac atgtaacacc taccttctta agtggatttt ttatttactg
     8461 cgtacttcga caaccattaa tggtgatcgt tttcacgcag gtaaatgacc cagtatgtca
     8521 acccaaccaa caaaccaccg ccgataatat taccgatcgt aaccggaatc aggttatcag
     8581 tgatgaagtt catcacggtc aggtgagaaa aattttccgg tgcagaaccg acagcggtcc
     8641 agaattccgg agatgcgaag tcgcggatta caatacccat cgggatcata aacatgtttg
     8701 cgatactgtg ctcaaaaccg ctggcaacaa acatcgcgac cggcagcacc ataatgaacg
     8761 ctttgtccat caggctgcgg ccagaataac tcatccatac tgccagacat accatcaggt
     8821 ttgccaggat accaagacag acggcctcaa taaaagtatg gtgcactttg tggtcggcgg
     8881 tttgtaggac gtttagtccc cattgaccat ttgcggtcat atactcgccg gaaagccaca
     8941 ttaaaagtac aaacagcagt gcgccgacca ggttgccaaa ataaacattt agccagtttt
     9001 tcgccaactg accccaggtg atgcgcccac tcgccttggc aacaacaatc aacactgtgg
     9061 aagtaaagag atcggctccg cagacaacac aaagaatcaa ccccagagag aagcaaatgc
     9121 cgccaaccag ttttgccatg ccgaagggca ttgtacctgt gccagtggtt gctgtgatat
     9181 agaagacgaa tgcgattgag atgaaaacac cggcggtaat cgccagatag aaagtcttaa
     9241 gcggatgttt cgttgcttta tagacacctg cctcttcggc cactttggcc attgcagcag
     9301 gaagtaaaag atcaaaaggg ttgtcagctt tcacactaac tctctcttta ttaagtcggc
     9361 gacgagatac taacaaagca ttatagatga gaaattgata tagatcatat ctcgcctggc
     9421 ttataggccc gtaactcgca tggtttttat gcaaatacgg agtaaatatt tgaata
//
LOCUS       DAWDRC010000074         9302 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3713_length_9302_cov_2.488050, whole genome shotgun sequence.
ACCESSION   DAWDRC010000074 DAWDRC010000000
VERSION     DAWDRC010000074.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9302)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3713_length_9302_cov_2.488050"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    699..708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8200..8209
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2570 a         2156 c         2077 g         2479 t
ORIGIN      
        1 tttaatattt atttttttca cattatagta gataaaaaca cgcattaaaa atgacttgta
       61 gaaaagctgc acaataaata gttcatattt aatctgttgc gtagaactac tattcaaaaa
      121 atcttattaa tgagataatg agattacatc gataacgttc tgattgcagg catacttatc
      181 tgggtgtgcc tctctttttt ttacaactgt acacggtggc gtatgacgag aaaatatctc
      241 acacaggatg aagtctacag gctgatggat gcagcccaga gcatgtcctt tcctgaaaga
      301 aatcgctgtc tgattatgat ggctttcatt cacggtttta gggccagtga acttcttgat
      361 ttacgtttat ccgatattga tgcatctgga aaacaactta atattcggcg tattaaaaat
      421 ggtttctcga caacacatcc tcttcttcct gatgaatata atttgatcaa gttgtggctt
      481 aagcagagga agttaattga aaacggagtt gaaggagact ggctttttct ctcccggaaa
      541 cgccgcccta tcagcagaca acattttttt tctatcattc gtgaggctgg aaaacgtgca
      601 ggattagctg taaaagcaca tcctcatatg ttacgtcatg cctgtggttt tgctctggct
      661 gacaatggtg tcgatacccg actaattcag gattatctnn nnnnnnnntc tgggtcatcg
      721 aaatattcag catactgtca gatacacagc cagtaatgct gctcgtttta aaggggtgtg
      781 gaaaaaaaag cctcgttaaa acagtacctt ctgattcaaa atgtcagatt cctacgtttt
      841 cctcaattct tccataaaaa aacagcccat tcaattaaat aaaacacata acatgttgat
      901 atttctagct aaagtaaaat aaccctatga tttgaaatga ttaatcacaa aggtcatacc
      961 agtgattaac tttcgcccac gttgttaaat aacacaaaat taaaaacaat aaaaatcaaa
     1021 caaattagat ttatattcca agaaaattaa atattcaggc tattaaacca aacctgacat
     1081 tttgaatcaa aatgtcaggt tttgtagcaa tggcgtggac gagtctgaat gaagaagcct
     1141 ctggttatta tcagtgcctg tcagttcact aggctggcac tggagtctct catcccaacg
     1201 gacaggtata tcgtgcgggt gtacagcaac gtcactacag aggttgaatt cgttttaaag
     1261 acctcctgtg gctacctgtt agctgatatc ccttcgcttc cttcggggga gttggttctt
     1321 ctggcttgtc tcgcccgttt gcggagtgat gccgggcaat ggcaggtctg cctgaccggt
     1381 gactccgact ggttcgataa tgctcctgct gcaagtctgt ataacggctt cccacgcatc
     1441 ggcaccatgc tgacagcgag tcgttatcag aatcaaatta tccgctggtt actacgtcct
     1501 ttgcccggcg atatgagcga tttctggctc ctgactgcac gggagttgag cgtgcttaag
     1561 attctgatgc agggcatgag tttcagtgag attgcccgtt atgaacaacg cagttcaaag
     1621 accctgcatg cgattgccac acgggcttta atgaaacttg gactgggaac actgagcgat
     1681 ttcaggctgc tctatacggg atgcggtgac agcagagtga aacggaatat acgactacat
     1741 gcaaaatata tcggacaaca ttccgctcat cgactcactc agtatgtgca gaacatggta
     1801 tccggaatcc tttatccaga gaaagttgcc gttggtgaat caataaattc tgacgccatt
     1861 tccggaaaag tactttccgg acaaagaaaa aatatatcta aaaaaagacc aggattgtta
     1921 tgacacagca gcgttgttac taccacccac tgcggcgcat acggactcgc tacagtgtag
     1981 gagccaaaat atttctactg ttctgggctg caggattggg aggcacgctg tacagcctgt
     2041 acagcgttct ggcgtactga catctgtgag ggaaggatgc gttacgttct tttctgtccc
     2101 tcaggaattg taccagcgca gttcgcagct ctatcaaccg gttctgtcgg caatgttacc
     2161 tgcatcagaa cagaagagga gttacgcaat aaattgcggc acaggccaca gagtgtcgtg
     2221 atatcggcag ggcgacctgc cgaatgcgct gaaatgtggt tccgatttta tcgggatcac
     2281 tcattcgtgg tggtgttgtg cgtcgcgccc ttttttctgc caccggatgt gagtatttct
     2341 ggcgttctca aaaacctgag gttactgaaa ccaggaatgt cagtagagcg tgtcatcagt
     2401 attgcaaatg cctctggtgg gttttcagga ctgaaacatg cagaaaattt gccagttatg
     2461 gactgttact ctgtctttat gaaagaggtg aataaccgca caaagacaat cgtcatgtca
     2521 gaacgcttcc cggaaaagca aaagaaggtg ttgtcgttgc ttcttgccgg acacagctgg
     2581 gaatacagtg cgcagtttct caaaacgggg ataagacaga tatggcttgc cgagcagtcg
     2641 ctaaaaaagc gttggggaat accggacagc atgtcattgc gggaagccct gctgctgagc
     2701 agcaataatt ttcacggtga tggaaatgca ttggaaacaa cgaatgccat gacattgcgt
     2761 gaaaacggaa ataccaacag gtattccgtg gtggtaaatg cagggacgca ggctttatct
     2821 ctgcataaat ataaataagt gggaaattga aattagttac cagaatggag aatttcttca
     2881 tgaaaaacag taaggcattt taccgcagcg cattagcgac agctattgtt atggctcttt
     2941 ctgcaccagc attcgctgct gataacgcgg tatcaactga ttcggttacg ctgaataaag
     3001 ataagacgac tctggatcaa gatgttgtta ttaacaatac agcagacaaa cagattacag
     3061 ccgtaacaat taatgcggca aatgaagacc tgaatgttac ttttgctggt catgatatta
     3121 ctgctgaatc aacggcagac aaaaaattcg ttgaaggtgt aaaagttagt ggtaacaaaa
     3181 atgttgtgat taatgctaca gactccacca tcacagctca aggtgaaggc acctatgtcc
     3241 ggactgcaat ggtcatttct tcaactggtg atgttgttat taatggcggt aatttcgttg
     3301 caaaaaatga aaacagtagt gcgacaggaa tatctctgga agggaccaag ggaaataatg
     3361 tcacgctcaa tggcacaacc ataaatgctc aaggtaataa gagttccagc aacgcctcca
     3421 cggcaatttt tgctcaaaag ggtagcttat tgaatagttt tgaaggtgat gcaaccgaca
     3481 acatcaccct tgctgactca aatattatta atggcgggat tgaaacaata gttactgccg
     3541 ggaataagac gggaattcat acagtcaacc tgaatattaa ggatggctca gtaattgggg
     3601 cagctaataa taaacaaaca atttatgcct ctgcttcggc acaaggcgca ggttcagcaa
     3661 cgcaaaattt aaatttgtct gttgctgatt caaccatcta ctctgatgtc ctagcccttt
     3721 ctgaaagcga gaattcagcc ggtaccacaa caaatgtaaa tatgaacgtt gcccgctctt
     3781 actgggaagg taatgcttat acgctcaata gcggcgataa agcgggtagt gatctggata
     3841 taaatctttc cgatagttca gtctggaaag gcaaagtttc aggggcagga gatgccagtg
     3901 tatctctgca aaacgggtct gtctggaatg ttacgggttc ctcaactgtt gatgctctgg
     3961 cagtaaaaga cagtacggtt aatatcacga aggctacagt caatactggc acgtttgctt
     4021 ctcaaaacgg cactctgatt gttgatgcct cttctgaaaa caccctggat atcagcggaa
     4081 aagcgagcgg tgacttgagt gtttacagtg cgggttcatt ggatcttatc aatgaacaaa
     4141 cggcatttat ttctaccggc aaagacagca ctctaaaagc cacaggcaca acggaaggtg
     4201 gtctgtatca atatgacctg acacagggag ctgatggtaa cttttatttc gtaaaaaaca
     4261 cgcataaagc atccaacgcc agctccgtga ttcaggcaat ggcagctgct ccggctaacg
     4321 tcgctaatct gcaggctgac acgctctccg cccgtcagga tgctgtccgt ctgagcgaaa
     4381 atgacaaggg tggcgtatgg attcagtact ttggcggtaa acagaaacat accaccgcgg
     4441 gaaatgcatc ctatgacctg gatgtaaatg gtgtaatgct gggtggtgat acccgcttca
     4501 tgactgaaga tggtagctgg ctggccggtg tggcgatgtc ttctgcgaaa ggtgacatga
     4561 ctaccatgca gagcaaaggt gacactgaag gttacagctt ccacgcttac ctgagccgcc
     4621 agtataacaa cggtatcttc attgatactg ctgcacagtt tggtcactac agcaacacgg
     4681 cagatgttcg cctgatgaat ggtggcggta ccatcaaagc tgactttaac accaatggtt
     4741 ttggtgcgat ggttaaaggc ggttacacat ggaaagacgg taatggcctg tttattcagc
     4801 catatgccaa actgtctgct ctgactctgg aaggtgtgga ttatcaactc aacggcgtgg
     4861 acgttcattc tgacagctat aactctgtgc tgggtgaggc cggtacgcgc gtgggttatg
     4921 acttcgctgt gggcaatgcg accgttaaac cttatctgaa tctggccgca ctgaacgaat
     4981 tctctgatgg caacaaagtc cgtctgggtg atgactctgt caatgccagc attgacggtg
     5041 cagcattccg cgtgggtgca ggtgtacagg ctgatatcac caaaaacatg ggagcatatg
     5101 caagccttga ctacaccaaa ggtgacgaca ttgagaaccc gctacagggt gtagttggta
     5161 tcaatgtgac ctggtaagaa taaagcagtc ggttcccctg tcgactgtta tacaccctgc
     5221 aggcagcagt atgctgcctg caggctataa atatccagta ctcatgatgc tggctttttg
     5281 ctgatattta ccctgaacat tcctgataca gactattaat acaatcttat ctgcgacaga
     5341 tttcattttc tggatatatt atgaatgaac aaatcaaaca ggatatcgat ctgattgaga
     5401 ttttatttta tctgaagaaa aagattcgtg ttatcctttt tattatagct atctgtatgg
     5461 caatggtgct gttgtttctg tatatcaata aagataatat aaaagtgagt tacagcctaa
     5521 aaataaatca gacaacacca ggcatacttg ttagctgtga tagcaataat aactttgcct
     5581 gtcagaccac aatgactgaa gatgttattc agcgaattac tacatttttt cagaccagcc
     5641 cagatgtcaa gaacagagaa ataaagctgg aatggtcagg agataagaga gatttaccaa
     5701 ctgctgaagc ggaaatatct cgcgtgcagg cgtctatcat taaatggtat acgtcagaat
     5761 accataatgg caggcaagtt ctcgatgaga tacaaacgcc ttcagcaatt aacagtgagc
     5821 tttatacaaa aatgatatac ctgaccagga actggtcact gtatccgaac ggtgatggct
     5881 gtgtcactat aagctcacca gaaataaaaa ataaataccc tgctgccatt tgcctggctc
     5941 tgggattttt tctaagtatt gtgatttctg taatgttttg ccttgtcaaa aaaatggtag
     6001 atgaatacca acaaaactct gggcagtaat aaacagcatt ctgacagaga gtgcctgtat
     6061 aaaactatag tgacagtgac cgggaaggga gcccgtcttc aggaatggat tcctgtcttt
     6121 ttaactgtaa atatcctggc attattaatg tcaggatatt tacagaaaag cagcataacc
     6181 atgacggaac ggggttaagt caggttgtcc ttaacgttat tcacaacctc gtataccgcc
     6241 ttcaggttta tgtccagact acatatctct ctcagcaatc ctcaataatc agatacaccg
     6301 ctttcaccgg accatgaacc ccgacgactt tgataagctc aatgtccgcc gttgaactgg
     6361 gaccactgat gatgttaata catgacggca ttcgttcgcc ggcctgcgct ttctgatgca
     6421 atttttctgc aagttgcgct acacgcggca ggattgtgct tttacgcagg ataaaaagag
     6481 aagattccgg gagcaggctc aatgaacgcc cgcgcccggc ggcggaaaaa agaaccacgc
     6541 ctcccgattc ggttaaacca tattcagcat atacgacgcc cactttagcc tgctctgctt
     6601 gtgatatgtt ctcaatacct ttcgccggat cccaaacaac ggcattgcat tcctgctgca
     6661 aacgttcgct aatccccaat tcctccagcc tcgtgtcacc actaatcacg accgactgat
     6721 ctcccagctc tttacacaga cgtattgcag cttctgccgc cttcgcctcg ctggtcagtt
     6781 cacagcgcgt caacataaca tcgctggcaa actggataaa cgcgtcacag cgctgctgtt
     6841 gggtaagttg ggtaagccgc tcgttagcat agttgttaag cggcgcatct tctgcttgcg
     6901 gttcaagtcg cagcgggcgc cccagtgcct gagcaacgtt attcaaaaat ttgctccgat
     6961 tatccattct ttttctcctg cgcctgatgt ttcttaaacc aactacggaa actctctccg
     7021 tcagcttcag gaagatcgcg tgcttccatc cagtcgctaa tcgcgccaaa tttgagtggt
     7081 gttttgccgc cattgataaa ccagcttgcc gcatgagcac cagccatcat cccaactttc
     7141 cacaatcctg gatgactatt ggcataagcg aacattttta tcgcccgttg ctctgctttt
     7201 gcggtgatcc ctttttcagc catcacccga cgatgacgca aaatcagttt tgacagcgga
     7261 atacgcaccg gacacacgct gtcacaagcg gtgcataaag agcaggcgta gggtaaatct
     7321 ttaaaatctt tatagccgcc aagtaacggg gaaatcaccg caccaattgg ccctggataa
     7381 atagagccat atccatgacc gccaatatgg cgatatgccg gacaagtatt catacaagcc
     7441 ccgcaacgaa tacagcgcag cacatcccga aattcagagg ccagcacctc agaacgcccg
     7501 ttatcgacaa taaccagatg aaactcttca ggaccatcaa cgtgccccgc ttcgcgcggt
     7561 cctgtcagcc aggtgttgta tcccgtcaaa cgtgcaccaa cggcactgcg cgccagcatg
     7621 gtgatcaata catctacctc tgcaaacgtg ggagcaatac gctccattcc catcactgca
     7681 atatgcgttt taggcagcgt ggtacacatt cgcgcattac cttcattggt caccaggcat
     7741 accgaaccgg tctctgccac cgcgaaatta cagccggtaa tacctatttc agcactgagg
     7801 aaatcttcgc ggattttttg ccgaatgaat aaggtcatcg cttcaggcgt ttccggcccc
     7861 tcatagccca gacgttcgtg tagcactcga cggatctgat ggcgatcttt atgaattgcc
     7921 gggaccacaa catgagaggg cggatcttga tccagctgga gaatgtattc acccagatcg
     7981 gtttcaatca cctgaatccc ggcatcctgc aacacatgat tgacaccaat ctcttcggtc
     8041 accatcgatt tagatttcac taccttccgg gcatttttgc gttgggcaac ctgtaaaatg
     8101 tagcgggtgg cgtcttcttt ggtttttgca aaatagacgt gaccgccgtt ttgtgtcact
     8161 ttttctgaga gctggtacag ataagcgtcg agattactcn nnnnnnnnna ttactcagaa
     8221 catgatcacg tatctgggcg gcccgatcgc gccactcctc ccagtgcccc aattcatcga
     8281 ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt tgccaccgct ttgcgcatga
     8341 tcggatcttc aatttgctga cgaatgcgtg tcttaaaatc tgtattactg gttttgatcg
     8401 acatctttat atcctcagcg gctcatcagc acttcagcaa tatgcatcac tttgactttc
     8461 tgcccttccc gttgtaatcg tccactgatg ttcagcaggc aactcacgtc agcaccaatt
     8521 aaatactcag ggcggacttc catcaggtgc acaacctttt ctttcaccat ctcgccggat
     8581 atttcggcca ttttgaccga gaacgtgccg ccaaatccgc agcaggtatc ctgttcagca
     8641 aaggtcaaca gctccagtcc acgcacattt ttcagcagcg taagtggttc gtccttcacc
     8701 cccagcttac gggcaaggct acaagatggg tgatacaccg ctctcccttg caaactggca
     8761 cctacatcga ctacccctaa tgtattaaca ataaaagagg tgagatcctg catacgcgcg
     8821 gcaacctttt cggcacgtga tgcccattca ggttcatccg ccagatacgt cgggtaactt
     8881 tttacggcat aggtgcaaga gccagccggt gaaataatgg gatcgtcgtt atcctccagt
     8941 gcggcgatca gatttttcat ccctggaatc gcttctttga tataaccact attgatcgca
     9001 ggctgaccgc agcatccctg tttctccggg aaatttacgc gacagccgag tttttccagt
     9061 agcagcacgg agtctcgtgc cattcttgat ttcagggcgt caccaatgca ggtgacaaag
     9121 aaattgacat tcacaactaa tactccatta cttcatgccc atttatgccc gatggttccc
     9181 cccttccggg ggatcaatca tcaataaata tggcgttctt ttttaattgg ctagcaacat
     9241 accacctctc atttgtatga caaatgataa tctttcatct aaacttcaaa tgcaaaaact
     9301 aa
//
LOCUS       DAWDRC010000075         9298 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3718_length_9298_cov_2.308017, whole genome shotgun sequence.
ACCESSION   DAWDRC010000075 DAWDRC010000000
VERSION     DAWDRC010000075.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3718_length_9298_cov_2.308017"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3699..3708
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4622..4631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6411..6420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2173 a         2462 c         2452 g         2181 t
ORIGIN      
        1 tgtcgcggag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
       61 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
      121 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
      181 ctccagtctg gccctgcacg cgccgtcgca aattgttgcg gcgataaaat ctcgcgccga
      241 tcaattgggt gccagcgtgg tggtgtcgat ggttgaacga agcggcgtcg aagcctgtaa
      301 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtgga ctgatcatta actatccgct
      361 ggatgaccag gatgccattg ctgtggaagc tgcctgcgct aatgttccgg cgttatttct
      421 tgatgtctct gaccagactc ccatcaacag tattattttc tcccatgaag acggtacgcg
      481 actgggcgtg gagcacctgg tcgcattggg tcaccagcaa atcgcgctgt tagcaggccc
      541 attaagttct gtctcggcgc gtctgcgtct ggcgggctgg tataaatatc tcacacgcaa
      601 tcaaattcag ccgataacag aacgggaagg cgactggagt gccatgtccg gttttcaaca
      661 aaccatgcaa atgctaaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
      721 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
      781 ctcggtagtg ggatacgacg ataccgaaga cagctcgtgt tatatcccgc cgttaaccac
      841 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
      901 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
      961 cacccttccg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1021 gctggcacga caagtttccc gactggaaag caggcagtga gcgcaacaca attaatgtga
     1081 gtcagctcac tcattaggca ccccagtctt tacactctat gtgtccggct cgtatgttgt
     1141 gtgaaattgt gagcggataa caatttcaca caggatacag ctatgactat gattacggat
     1201 tctctggccg tcgtattaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1261 cgccttgcgg cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1321 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     1381 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgacgccga tactgtcgtc
     1441 gtcccctcaa actggcagat gcacggttac gatgcgccta tctacaccaa cgtgacctat
     1501 cccattacgg tcaatccgcc gtttgttccc gcggagaatc cgacaggttg ttactcgctc
     1561 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
     1621 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     1681 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
     1741 gtgatggtgc tgcgctggag tgacggcagt tatctggaag atcaggatat gtggcggatg
     1801 agcggcattt tccgtgacgt ctcgttgctg cataaaccga ccacgcaaat cagcgatttc
     1861 caagttacca ctcgctttaa tgatgatttc agccgcgcgg tactggaggc agaaattcag
     1921 atgtacggcg agctgcgcga tgaactgcgg gtgacggttt ctttgtggca gggtgaaacg
     1981 caggtcgcca gaggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggcggttat
     2041 gccgatcgcg tcacgctacg cctgaacgtt gaaaatccgg aactgtggag cgccgaaatc
     2101 ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca
     2161 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     2221 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     2281 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     2341 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
     2401 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     2461 ctgaccgatg atccgcgctg gctacccgcg atgagcgaac gcgtaacgcg gatggtgcag
     2521 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
     2581 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccttcccg cccggtacag
     2641 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     2701 gtggatgaag accagccctt cccggctgtg ccaaaatggt ccatcaaaaa atggctttcg
     2761 ctgcctggag aaatgcgccc gctgatcctt tgcgaatatg cccacgcgat gggtaacagt
     2821 cttggcggca tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     2881 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg
     2941 tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt
     3001 ctggtctttg ccgaccgcac gccgcatccg gcgctgacgg aagcaaaaca ccaacagcag
     3061 tatttccagt tccgtttatc cgggcgaatc atcgaagtga ccagcgaata cctgttccgt
     3121 catagcgata acgaactcct gcactggatg gtggcgctgg atggcaagcc gctggcaagc
     3181 ggtgaagtgc ctctggatgt tggcccgcaa ggtaagcagt tgattgaact gcctgaactg
     3241 ccgcagccgg agagcgccgg acaactctgg ctaacggtac gcgtagtgca accaaacgcg
     3301 accgcatggt cagaagccgg acacatcagc gcctggcagc aatggcgtct ggcggaaaac
     3361 ctcagcgtga cactcccctc cgcgtcccac gccatccctc aactgaccac cagcggaacg
     3421 gatttttgca tcgagctggg taataagcgt tggcaattta accgccagtc aggctttctt
     3481 tcacagatgt ggattggcga tgaaaaacaa ctgctgaccc cgctgcgcga tcagttcacc
     3541 cgtgcgccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc
     3601 tgggtcgaac gctggaaggc ggcaggccat taccaggccg aagcggcgtt gttgcagtgc
     3661 acggcagata cacttgccga cgcggtgctg attacgacnn nnnnnnnntt acgaccgccc
     3721 acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg
     3781 gacacggcga gatggtcgtc agtgtaggtg ttgaagtagc gagcgataca ccgcatccgg
     3841 cgcggattgg cctgacctgc cagctggcgc aggtcgcaga gcgggtaaac tggctcggcc
     3901 tggggccgca agaaaactat cccgaccgcc ttactgcagc cagtttttac cgctgggatc
     3961 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg
     4021 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca
     4081 gccgctacag ccaacaacaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag
     4141 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga
     4201 gcccgtcagt atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct
     4261 ggtgtcaaaa ataataataa ccgggcaggc tatgtctgcc cgtatttcgc gtaaggaaat
     4321 ccattatgta ctatttaaaa aacacaaact tttggatgtt cggtttattc tttttctttt
     4381 acttttttat catgggggcc tacttcccgt ttttcccgat ttggctacat gacatcaacc
     4441 atatcagcaa aagtgatacg ggtattattt ttgccgctat ttctctgttc tcgctattat
     4501 tccaaccgct gtttggtctg ctttctgaca aactcgggct gcgcaaatac ctgctgtgga
     4561 ttattaccgg catgttagtg atgtttgcgc cgttctttat ttttatcttc ggaccactgt
     4621 tnnnnnnnnn natacaacat tttagtagga tcgattgttg gtggtattta tcttggcttt
     4681 tgttttaacg ccggtgcgcc agcagtagag gcatttatcg agaaagtcag ccgtcgcagt
     4741 aatttcgaat ttggtcgcgc gcggatgttt ggctgtgttg gctgggcgct gtgtgcctcg
     4801 attgtcggca tcatgttcac catcaataat cagtttgttt tctggctggg ttctggctgt
     4861 gcactcatcc tcgccgtttt actctttttc gccaaaacgg atgcgccctc ttcagccacg
     4921 gttgccaatg cggtaggtgc caaccattcg gcatttagcc ttaagctggc gctggaactg
     4981 ttcagacagc caaaactgtg gtttttgtca ctgtatgtta ttggcgtttc ctgcacctac
     5041 gatgtttttg accaacagtt tgctaatttc tttacttcgt tttttgctac cggtgaacag
     5101 ggtacgcggg tatttggcta cgtaacgaca atgggcgaat tacttaatgc ctcgattatg
     5161 ttctttgcgc ccctgatcat taatcgcatc ggtgggaaaa atgccctgct gctggctggc
     5221 actattatgt ctgtacgtat tattggctca tcgttcgcca cctcagcgct ggaagtggtt
     5281 attctgaaaa cgctgcatat gtttgaagta ccgttcctgc tggtgggctg ctttaaatat
     5341 attaccagcc agtttgaagt gcgtttttca gcgacgattt atctggtctg tttctgcttc
     5401 tttaagcaac tggcgatgat ttttatgtct gtactggcgg gcaatatgta tgaaagcatc
     5461 ggtttccagg gcgcttatct ggtgctgggt ctggtggcgc tgggcttcac cttaatttcc
     5521 gtgttcacgc ttagcggccc cggcccgctt tctctactgc gtcgtcaggt gaatgaagtc
     5581 gcttaagcaa tcaatgtcgg atgcggcgcg agcgccttat ccgaccaaca tatcagggcg
     5641 gaatgatcgc aatgaacatg tcgatgacag aaagaataaa agcaggcaag ctatttaccg
     5701 atatgtgcga aggcttacct gaaaaaagac ttcgtggaaa aacgttaatg tatgagttta
     5761 atcactcgca tccatcagaa gttgataaaa gagaaagtct gattaaagaa atgtttgcca
     5821 cggtagggga aaacgcctgg gtagaacccc ccgtctattt ctcttacggt tccaacatct
     5881 atataggccg caatttttat gcaaatttca atttaaccat tgtcgacgac tacacggtaa
     5941 cgatcggtga taacgtactg attgcaccca acgttactct ttccgttacg ggacacccgg
     6001 tacaccatga attgagaaaa aacggagaga tgtactcttt tccgataacg attggcaata
     6061 acgtctggat cggaagtcat gtggttatta atccaggcgt caccatcggg gataattctg
     6121 ttattggcgc gggtagtgtc gtcacaaaag acattccacc aaacgtcgtg gctgctggcg
     6181 ttccttgtcg ggttattcgc gaaataaacg accgggataa gcaatattat ttcaaagatt
     6241 ttaaagttga gtcttcagtt taaataagca aaattgcctg atgcgctccg cttatcaggc
     6301 ctacaatttc agagatctac gtaggccgga taaggtgttc acgccgcatc cggcataaac
     6361 tacgctcgcg gtaacccagc cgattaccgt ttgtaatcga tggcttctgg nnnnnnnnnn
     6421 gcttctggct gctccagata tacggtggtt tgtgccggtt ttgtgctggc aatcaccttg
     6481 ccgccacgta ccgaataacg taccggaacc tgacggcgca gcgcatcaaa cccattttca
     6541 gccggcagga taatcaggtt ggcgctgttt ccggcggcaa tgccgtaatc ctgcaaattc
     6601 aacgtcctgg cgctgtggtg ggtgattaaa ttcaggccgt cgttaatctg cccgtagccc
     6661 atcagctggc aaacatgcag ccccatatgc agcacttgca gcatattcgc cgttcccagc
     6721 ggataccacg gatcgaagac atcatcgtga ccaaagcaga cgttaatgcc ggactccagc
     6781 atctctttaa cgcgcgtgat gccgcgacgt tttggatacg tatcgaaacg tccttgcaga
     6841 tgaatattga ccaacgggtt ggcgacaaag ttaataccgg acattttcag caaacggaac
     6901 aggcgcgaag tatacgcccc gttataggag tgcattgccg tagtatggct ggcggtgact
     6961 cgcgcgccca tgccttcacg gtgcgccagg gcagcaacgg tttcgacaaa gcgcgactgc
     7021 tcgtcatcga tctcatcaca gtgaacgtcg atgagacggt cgtatttttg cgccagggcg
     7081 aaggttttat gcagcgactc cacgccgtat tcacgggtaa attcaaaatg cggaatcgcc
     7141 ccaacaacat ctgcccctaa gcgtagcgcc tcttccagca acgcttcacc gttgggatac
     7201 gacaaaatcc cttcctgcgg gaaggcgacg atttgcagat caatccacgg cgcaacttcc
     7261 tgtttcactt ccagcatcgc tttcaacgca gtcagcgttg cgtcggagac atcgacatgg
     7321 gtacgcacat gctgaatgcc gttggcaatc tgccatttca gcgtttgcca tgcgcgttgt
     7381 ttcacatcga catgagttaa tatctctttg cgctcggccc agcgttcaat gccttcaaac
     7441 agcgtgccgg actgattcca gttcggttgt ccggcggttt gcgtggtgtc caggtgaata
     7501 tgcggctcca caaacggcgg taaaactaaa ccttgttcgg catccaggct gttttcagtt
     7561 atgggcatca ccccggattg cgcatcaatg gcgctgattt ttccgtcgtg aaaataaatc
     7621 tgccacagcc cctctttgcc gggtaaccgg gcgttaataa ttgtttgtaa agcgttattc
     7681 gacactgtta gcctccacat gcgtcattgc tgctgtcgtt ttacgattca aaatcgggtt
     7741 aaggatcaga tagctcagcg cgccacctaa taccgcgttg accggaacaa ttcccggtaa
     7801 ccagtggcct gcggcaatcc ccagcgcgac cgccagaatc gccacccaat tgacactcat
     7861 catacgcgtg gtcgcaaagt gctcatagcg gcgacggttc atcagataat cagcgatgat
     7921 cacgccgccc actggaggaa tagctgccga aaggaaggtc aaccagccga caaaattgtt
     7981 atacagccat aatgcgcaga ccgtaccgat aataccgtta atcaccgaaa gggttttgct
     8041 cgacataccg gtaatgttgg cgaaacctaa acccgacgca tagagcgcgt tatcgttggt
     8101 ggtccagata ttcagcccca gcaccacaat cgcaggcagc agcaggccct gagcaatcat
     8161 cacatcagag atatccgcca tccccagtgc cgcagcgccc gctgcaccga aaataaacat
     8221 caacgagttg ccgaggaaaa aggccaccat cgccaccagc accgccagtt tggcattgcg
     8281 accaaaccgg acaaagtcag cggtgagcgt gcccgcactg ataaatgacc ccacaaccag
     8341 cgccagcgcg acattgaaat ctaacggttg tgcgggaacg accgctttta atacgtccag
     8401 gccgcccatg ccgttaacgg ccagccacac ggaataaccg ccaaggcagg cgatagccgg
     8461 aaccgcaatc accgaaagaa ccgtcagcgc cgaaatgcca aagaagacgg tgacggtcat
     8521 cagtaaaccg gagacggcaa tcagcaaatt aatatccagc ccggttgcct tgcccaccgg
     8581 aatagaaaac atcgccacgc caacgccaaa ccagccaacc tgggtgccgc ccagcagcag
     8641 tgaaggcaac catgagcctt taacaccaaa cgagaagcga gcaagaagat gggtggtcag
     8701 gccggttttt gcgccaatgt aaccaagaaa tgaagtgtaa ataccgagga gaagattacc
     8761 gatgagaact gcgaggaaga aatcatgata gctaagaccg gttccgagag tgccgccggt
     8821 ccacatactg gcggaaaaga aggttaatcc cagcatgacg aacgtcaatg ccaatacccc
     8881 tttccgcgcc gactgcggga ctggcccctg gctaaagttg ttatcttgcg acacgaaatt
     8941 cccccttttt gctgtttgaa acccaaaaaa tccgccgcat tctattcatc tgaagataaa
     9001 aagcaatcgt tttcgtaggg ggatatattt tttatatgaa tgagtgcaaa aaggcggggg
     9061 aaacttatct gtgtaaaaac agtgggtagg catgataaga cgcgtcagcg tcgcatcagg
     9121 catctgcgca tggtgtcgga tgcggcgcga gcgccttatc cgacctacgg ttcaggcctg
     9181 taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcacggtgt cggatgcggc
     9241 gcgagcgcct tatccgacct actcttccat cgcctggcgg atctgatcca acgacgcc
//
LOCUS       DAWDRC010000076         9281 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3725_length_9281_cov_2.326577, whole genome shotgun sequence.
ACCESSION   DAWDRC010000076 DAWDRC010000000
VERSION     DAWDRC010000076.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3725_length_9281_cov_2.326577"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2348 a         2030 c         2369 g         2534 t
ORIGIN      
        1 ttatatattg aatatttagc gcgctaacaa tagagagagt ctacccattt ggggttaata
       61 attattaatc catatcaata gaagggtatg agtaataagg tgggattatg ttgtatgttc
      121 aaatcgccgg atgtgtcgta tccggcgttc agtcgataat gtgttactgc ggttcagcag
      181 gcgcgttatc ctgggtagac tgcgcgggag cagagacgtt accgctggtg gtacgggtgt
      241 agagaatttt atgcgtatca ttagcgcaat gcccgacgac ctgggaatca ggctgatcaa
      301 cctggtcatt gggtacaata cttaacgtga agctgctttc gggtacgcca ttattgataa
      361 tgcgctgtga tatatcgctc tgtatgcgct cacaggatcc cggcgcggcg agtaccgcgg
      421 gtgaggcgag ggcgagcaga agcgcggcac agcaggttga gagtttcatc ataagctcct
      481 tacgcgaaga taacttcttt aagcatagca tttaacgtgt aaagtactgt atttgctact
      541 atgattgaga atcatctcta ctctcaggtg actgttgtga aatacaaatt actgccatgc
      601 ttactcgcga tattactcac aggatgtgac cgcacagagg taacactttc atttaccccg
      661 gagatggcca gtttctctaa tgaattcgat tttgatccgc tgcgtggtcc cgtaaaagat
      721 ttcactcaga cattaatgga tgagcaaggt gaagtgacga aacgtgtttc ggggactttg
      781 tcggaagaag gctgttttga ttctctcgaa ttactggatc tggaaaataa taccgtggtt
      841 gccctggtac tggacgccaa ttattaccgt gatgccgaga cgctggagaa aagagttcgt
      901 ttgcagggaa aatgccagct agcagaatta ccttctgccg gggtgagttg ggaaaccgat
      961 gataatggtt tcgtgattaa agccagcagc aaacagatgc agatggaata tcgctatgat
     1021 gatcaggggt atccgctggg taaaaccacg aaaagtaacg ataaaacatt atctgtcagc
     1081 gccacgccat caacggaccc gatcaaaaaa ttagattaca cagcggttac tttactgaat
     1141 aatcaacggg ttggaaatgt aaaacagagc tgtgaatatg acaatcacgc caatccggtg
     1201 gactgtcagc taatcattgt tgatgaagga gtaaaacccg ccgtcgaacg ggtttacacc
     1261 atcaaaaata cgatcgatta ttattaatac tattgtgcgg tcggcttcag gagagtctga
     1321 cccggtgttt tgtgctcagc cagatactga tgctggaata tacacatgcg aatggcatta
     1381 cgatattgac cattaataaa gaactcatgc atcaattcac cttcaaccgt aaagccaagc
     1441 ttgcggtaaa tgtgaatcgc tttttcattc tctttatcaa cgatcagata cagcttatag
     1501 agattgagaa cggtgaagcc atagtccatt gctaatttgg cggcacgggt tgccagacct
     1561 ttcccctgat actccgggga gataattatc tgaaattctg cgcggcgatg aacatggtta
     1621 atttccacca gctccaccag accggctttt tcgccgtcac attccaccac aaagcgccgt
     1681 tcgctctgat cgtgaatatg cttatcatac agatcagaga gttcaacaaa ggcttcgtag
     1741 ggttcctcaa accagtaacg catcacactg gcgttattgt cgagttgatg tacatagcgt
     1801 aaatcttcac gctccagcgg gcgtagctta acactgtggg cgcttggcat aacgtgtcct
     1861 tacattcctt aaatcaataa caggttaggg gataataacg cggccagttc gacggtccag
     1921 gcagcgcaaa gtatttggct cccagtaggc attgatgttg gcgctttgct cacatttatc
     1981 gcggttatca aaagcagcgt cggctttatc ccactctttt tcagtgcgtt tattcacttt
     2041 ctggcgcaga ttacgcgtgt cattccattg ctctttttcc atagcggcgc gctggcggct
     2101 ttgtgcactg tcgccagact caatcaccag tttgttagtt tcggcatgaa cagttgtgct
     2161 caatgccagt gcgcaaggca gcagaaaagc gagcaggccg attcgtttgc tgagagtgat
     2221 tttcataatt cattccctgt atgaatgatt aaggctgatt ctacaccatc cactgcgaac
     2281 gcaaaacgtt caaggagggt gtttatattg atgatattat gtcgccctat aactatacgt
     2341 gatgtcaata agtgacaaag atgattaaaa caacgttact attttttgct actgcgctgt
     2401 gtgaaattat tggatgcttt ttgccctggc tgtggttgaa gcgaaacgcc agtatctggc
     2461 tgttgcttcc ggcggggatt tcactggcgc tgtttgtctg gttgttaacg ttgcatcctg
     2521 cggcgagtgg gcgtgtttac gcggcttatg gtggcgttta tgtctgcacg gctttgatgt
     2581 ggttgcgcgt tgtggatggc gtgaaactga ctctttatga ctggacgggg gcgttgattg
     2641 cgctttgcgg catgttgatc attgttgcgg gctgggggcg cacgtaggaa cataaatcca
     2701 ttttatcaat aagatacgag gaagtgtcag ctgacaaaag gtattctatt tcatcttttg
     2761 tcaaccattc acggcgcaaa tatacgcctt tttttgtgat cactccggct ttttttgatc
     2821 ttcatacttg tatggtagta gctcagttgc gtagatttca tgcatcacga caagcgatgc
     2881 aaggaatcga acatgaagat cgtaaaggct gaagtttttg ttacctgtcc ggggcgtaat
     2941 ttcgtcacat taaaaatcac cactgaggac ggtattacgg gccttgggga tgccactcta
     3001 aatggacgtg agctttccgt ggcctcttat ttgcaggatc acctttgtcc gcagcttatt
     3061 ggtcgcgatg cacaccgtat cgaagatatc tggcagtttt tctataaagg tgcttactgg
     3121 cgtcgcggtc cggttacgat gtcggccatt tcagcggttg atatggcgct gtgggatatt
     3181 aaagccaaag ctgccaacat gccgctttac cagttactcg gcggcgcgtc tcgtgaaggg
     3241 gtgatggttt attgccatac caccggtcac agtattgatg aagctctgga tgattatgcc
     3301 cgtcatcagg agctaggatt caaagccatc cgcgtgcagt gcggaatccc tggtatgaaa
     3361 accacctacg gcatgtcgaa aggtaaaggt ctggcttatg aacccgcaac caaaggacag
     3421 tggccggaag agcagctgtg gtcgacggag aaatacctcg atttcatgcc gaaattgttt
     3481 gacgcggtac gtaacaagtt tggttttgat gaacatctgc tgcatgacat gcaccatcgc
     3541 ttaacgccta ttgaagcggc gcgctttggt aagagcattg aagattatcg catgttctgg
     3601 atggaagacc cgacgcctgc ggaaaaccag gaatgcttcc gtctcattcg ccaacatacc
     3661 gtcacaccca tcgcggtggg tgaagtcttc aacagcatct gggactgcaa acaactgatt
     3721 gaagagcaac tcatcgatta tatccgcacc acgctgaccc atgcaggcgg aattaccggt
     3781 atgcgtcgga ttgccgattt tgcttcgctg tatcaggtac gtactggctc acacggtcct
     3841 tccgatttgt caccagtctg catggctgcg gcgctgcact ttgatctgtg ggtccccaat
     3901 ttcggtgtcc aggaatacat gggttattcc gaacaaatgc tcgaagtctt cccgcacaac
     3961 tggactttcg ataacggcta tatgcatccg ggagaaaaac cgggtcttgg catcgaattc
     4021 gatgaaaagc tggcggcgaa atatccctat gaacctgctt atctgccagt cgcacgtctg
     4081 gaagatggca cgctgtggaa ctggtaagga gtaaggtaat gaaaagcatt ttaattgaaa
     4141 aaccgaatca actgtcgatt atcgaacgtg aaatacccac cccgtcagcg ggtgaagtac
     4201 gagtaaaagt gaaacttgcc ggaatttgtg gttcagatag ccatatttac cgtgggcata
     4261 atccttttgc gaaatatccg cgcgtcattg gtcatgaatt ctttggcgtc attgatgcag
     4321 tgggtgaagg cgtggaaagc gccagagtcg gtgaacgcgt tgctgtcgat ccggtggtca
     4381 gctgtgggca ttgctatccg tgctctatag gtaagccgaa cgtttgtacg acacttgctg
     4441 tattaggtgt gcacgctgac ggtggtttca gtgaatatgc tgtggttccg gcaaaaaatg
     4501 cgtggaaaat tcctgaagct gtggccgatc aatatgcggt gatgatcgaa ccttttacca
     4561 ttgcggctaa cgttaccggt catggtcaac cgactgaaaa tgataccgtt ctggtttacg
     4621 gtgccggccc aatcggcctg acgatcgttc aggtattaaa aggcgtctat aacgttaaaa
     4681 atgtgattgt tgccgatcgc attgatgaac gactggaaaa agcgaaagag agcggggcag
     4741 actgggcgat taataacagc cagacaccgc ttggcgagag tttcgctgaa aaaggcatca
     4801 agccgacttt aattatcgat gccgcttgtc atccttcaat cttgaaagag gccgtaacgc
     4861 tggcttctcc agcggcacgt attgtattga tgggcttctc cagtgaaccg tctgaagtga
     4921 ttcagcaagg aattaccgga aaagaactct ctattttctc ttcacgctta aatgcaaata
     4981 aattcccggt cgttatcgac tggttaagta aagggttaat taaaccagaa aaactaatta
     5041 cccatacatt tgatttccag catgttgcgg atgccattag tttatttgaa caggatcaaa
     5101 aacattgctg caaagtctta ctcacttttt ctgaataata ccaataacgg cgagtaagta
     5161 gtacgcatct tacctctttt ttagagataa ccattatgac aatagaaaaa cacgaaagaa
     5221 gtactaagga tttggtgaaa gcagcagtat cgggatggtt gggcactgcg cttgaattta
     5281 tggacttcca gttatattcg ctcggcgcag cgttagtgtt tcatgaaata ttttttcctg
     5341 aatcatcaac ggcaatggcg ttaattctgg caatgggaac ctatggtgca ggttatgtgg
     5401 cacgtattgt cggagcattt attttcggca aaatgggcga cagaataggg cgtaaaaaag
     5461 tgctctttat taccatcacc atgatgggga tctgtaccac cttaattggt gtgttaccga
     5521 cttatgcaca aattggtgtt tttgccccca tcttgctggt gacgctgcgt attattcagg
     5581 ggttgggtgc aggtgcggaa atttccggtg ccggtacgat gctggcggaa tatgcgccaa
     5641 aaggtaagcg cggaattatc tcctcatttg tggctatggg aactaactgc ggaaccttga
     5701 gcgcaacggc aatctgggcc tttatgttct tcattctcag taaagaggaa ctgctggcgt
     5761 ggggatggcg tataccgttc ctggcgagcg ttgtcgtgat ggtctttgct atctggttgc
     5821 gtatgaatct gaaagaaagc ccggtctttg agaaggttaa cgacagcaac caaccgacag
     5881 caaaacctgc acctgctggt agcatgttcc agagcaaatc cttctggctg gcaacagggc
     5941 tgcgttttgg tcaggctggt aactccggtt taattcagac tttccttgca ggctatttag
     6001 tgcagacgtt attgtttaac aaagcaattc caacagatgc attgatgatc agttcgattc
     6061 tcggctttat gaccattccg ttccttggtt ggttatccga taaaattggt cgccggatcc
     6121 cgtatattat tatgaatacc tctgcgattg tgctggcatg gccaatgctt tctatcatcg
     6181 tagataaaag ctatgccccg agcaccatta tggttgcact gattgtgatt cataactgtg
     6241 cggtgctggg attatttgct ctggaaaaca ttaccatggc agaaatgttc ggctgtaaaa
     6301 accgctttac ccgaatggct atttctaaag aaattggtgg tcttatcgct tccggttttg
     6361 gtcctatcct ggcggggatt ttctgcacca tgacggaatc ctggtatccg atcgccatta
     6421 tgatcatggc atattctgtg attggtttaa tctctgcgct gaaaatgcca gaagtgaaag
     6481 accgtgattt aagtgcgctg gaagacgccg cagaagatca accacatgtt gtaagagctg
     6541 cgcaaccgtc cagaagtctg taaatcctca atcccttctc ttaccggaga ggggattttt
     6601 attcatataa caaaacatgt agcttgctat atttaatttt aaggaattgt tatgggaaat
     6661 catttgttat cagcaaaagc gacgctccct gtttatgatc gtaataacct ggccccaaga
     6721 attgttcatt taggctttgg tgcatttcac cgtgcacatc agggcgtgta tgccgatatt
     6781 cttgctacgg aacatttcag tgactgggga tattatgagg tcaacttaat cggcggcgaa
     6841 cagcaaattg ccgatttaca acagcaagat aatctttata ccgttgcgga aatgtcggct
     6901 gatgcgtgga cggctcgcgt cgttggcatc gttaaaaaag ccttgcacgt acaaatagat
     6961 ggcttagaaa ccgtgttggc tgcgatgtgt gaaccgcaaa tcgcgattgt ctctctgaca
     7021 atcaccgaaa aagggtattt ccactctccg gcgaccggac agttaatgct cgatcacccg
     7081 atggtcgttg ccgacgtgca aaatccccac cagccgaaaa ctgcaacagg ggtgattgtt
     7141 gaggcgctgg ctcgccgtaa agcggcagga cttcccgcat ttaccgtcat gtcatgtgac
     7201 aacatgccag aaaacggtca tgttatgcgt gacgttgtca cttcctatgc gcaagccatt
     7261 gatgtaaaac tggcacaatg gatcgaagat aacgtgactt tcccatcaac aatggtggac
     7321 cgtattgtgc ccgcagtgac agaggatacg ctggcgaaaa tcgaacaact taccggtgtg
     7381 cgcgatgcgg caggcgttgc ctgtgaacct ttccgccagt gggtaataga agataacttt
     7441 gttgccggac gtccggaatg ggaaaaagcg ggagccgaac tggttagtga tgtgctgcct
     7501 tatgaagaga tgaagttgcg catgctcaat ggcagtcatt cattcctggc gtatctgggg
     7561 tatcttgccg gatatcagca cattaatgac tgcatggaag atgaacatta tcgtcatgca
     7621 gcgtatgcct taatgttgca ggaacaagcg ccgacgctga aagtgcaggg cgttgatttg
     7681 caagattacg ctaaccgatt aattgaacgc tatagcaacc cggcgttacg tcatcgaacc
     7741 tggcagattg cgatggatgg cagccagaaa ttgccacagc ggatgttgga ttctgttcgc
     7801 tggcatctgg cgcatgacag caagttcgat ctgctggcgc tgggcgtcgc gggttggatg
     7861 cgttatgtcg gtggtgttga tgaacaggga aatccgatag aaatcagtga tccactgtta
     7921 cctgttattc agaaggcagt acaaagtagt gctgaaggga cagcgcgcgt ccagtcattg
     7981 ctggcaatta aggcgatctt tggtgatgat ttaccaggca atagtttgtt tacgacaaaa
     8041 gtaacggaag cgtacttgtc tttattagcg catggcgcga aagcgaccgt ggcgaaatat
     8101 tccgtgaagt aaaaaaagca atgccgccat cggcggcatt cacacgctta gttcatgccg
     8161 agacgaatca ggtccagtgg tgccagtttc tcttcacaac cttcaacaac tacggttttt
     8221 ccgcggcgga tttgctcagc ggtcagacct tcagtatcaa tcgacaggat cttgccagtg
     8281 tgaccggtgc cgctaaccat aacacggctg ccagtggtga ttgcgttacg gttacgatcg
     8341 taggtcgtca taggtgtttt ctcctttctg atttacagtc gaagcccacg taacgggcgc
     8401 aatataaata ctcctgtcga gtgaaatatt ttttgttttt gatcaagctc acatcttttt
     8461 tgatgcatgc atgagccata agtcggggga aaagactctc accccggatg ggatgagagg
     8521 aaatgaatta ctcttcgcta aaccagtcgc tgttttcctg gcggatctgg cgcacggatt
     8581 cgctgatttc ctgcaaatgt tgcgtcattg cacgttctac cgcatcgcca tcacgttttt
     8641 gcagggcaga gaaaatatca agatgctggc gtaacagcat ttctggtgga gaaacgtggt
     8701 cgaagctcat atagcgcacg cgatcaacgg tcgctttcag gttctcaatg gtatcccacg
     8761 ccagttgaca gtcggcaatc tgcgtcagga gttgatggaa gttgtcatca agttcaaaaa
     8821 aatcatccag ttgcttgcgc tcaatggcaa tgcgttgctg gtgaagattt tgttccagtt
     8881 ggtagcactg gctttcggta atcatgctcg ccgcccgacg cgccaccgcg cactcaatgg
     8941 cctgacggat aaaactgccg ttgcgcacct gggccatgga aattttgttg acgtagctgc
     9001 cacgctgcgg acgaatttga atcagaccgt tttccgccag tttaataaag gcttcgcgaa
     9061 ccggctggcg tgacacattg aaacgaacag aaacttcttt ttccgacaac ggtgtgcctg
     9121 gtgcaatcag gcaatggaca atgtcgcggc gaagaatacg ataaatctgc tgattgacag
     9181 gctgtgtggg attaagttgc gtttcgacgg tcattcgttc ttgcttgtga gtgagttaac
     9241 tgcatgagtc taccactttt ttgtgacaag caataacccg g
//
LOCUS       DAWDRC010000077         9280 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3726_length_9280_cov_2.172141, whole genome shotgun sequence.
ACCESSION   DAWDRC010000077 DAWDRC010000000
VERSION     DAWDRC010000077.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9280)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9280)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3726_length_9280_cov_2.172141"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5733..5742
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6126..6135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2124 a         2559 c         2277 g         2300 t
ORIGIN      
        1 tatcaagtaa gagatggtgc atccgggagg attcgaacct ccgaccgctc ggttcgtagc
       61 cgagtactct atccagctga gctacggatg caaatggcgg tgaggcgggg attcgaaccc
      121 cggatgcagc ttttgaccgc atactccctt agcaggggag cgccttcagc ctctcggcca
      181 cctcaccaca cgcctcttac gagtgcttcg aagaacttgt ttatcgctca tcgtcgctgc
      241 gtggcgcaca tattactttc tgagacttat aagtcaaaca atttttccca cacttttatc
      301 gtttgcacac ttcacgctca attagtctgt aaaaacgtca aaaagagtgt tttatcaaca
      361 gaaaaacgga ggtctgacag atagcagtaa tgcaaaaaaa tggagactta agttgaatga
      421 acgggagtaa agcgaaaaaa caatggagtg aaagagaaat tttgagggtg cgtctcaccg
      481 ataaagatga gacgcggaaa gattagtaac tggactgctg ggatttttca gcctggatac
      541 gctggtagat ctcttcacgg tgaacagaaa cttccttcgg ggcatttacg ccaatacgta
      601 cctggttgcc ctttacccct aaaactgtca cggtgacctc atccccaatc atgagggtct
      661 caccaactcg acgagtcaga atcagcattc tttgctcctt gaaagattaa aagagtcggg
      721 tctctctgta tcccggcatt atccatcata taacgccaaa aagtaagcaa tgacaaacac
      781 attacatcta ataagcagtc atggcattac attctgttaa acctaagttt agccgatata
      841 cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag
      901 tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag cctttcacac
      961 tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac
     1021 gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc cctgctttca
     1081 cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct tcggctaccg
     1141 ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc atggtacgca
     1201 acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca ttaacatcaa
     1261 ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta agctgttgta
     1321 actctttttc cagctgacgc gtacgttcca gtactgaacg cactttatca gccagattat
     1381 tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcactgtct gcatgaacgg
     1441 tggcgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca gcagtacccg
     1501 attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga gtaccgccac
     1561 acagctcggt ggagaaatcg cccatgctca gcacgcgtac gcgctcatca tacttctcgc
     1621 caaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg ttggtttcga
     1681 tcggcaagtt acggcgaatc tgtgcgttca ccaggtcttc gaccgcacga atctcttctg
     1741 gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac tttgtcgtta accagtgaac
     1801 ctttctgcga tacatgagta cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg
     1861 cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc tgcacagcgt
     1921 cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc tggccgtatt
     1981 tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct ttatcgccaa
     2041 cctgaccgcc ggattccgca tagaatggcg tttgatccag cacgaccaca gcttcctggc
     2101 ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact ttgccgttca
     2161 gttccagatg gtcatagcct ttaaattcag atgcactgtc aacacggatc attgcgttgt
     2221 aatcggcacc aaagccgctg gcttcgcgcg cacgacgacg ctgctcttcc attgctgctt
     2281 caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacgtcagcc gtcaggtcaa
     2341 ccgggaagcc gtaggtgtcg tacaggcgga aagcagtttc accatccagc gtatcaccag
     2401 aaagttttgc cagctcttcg tccagcagcg ccagaccgcg ctccagagta cgggcaaact
     2461 gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac
     2521 ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag gtttctttcg
     2581 cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt acataaccac
     2641 ggttttcgtt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt
     2701 cagcgattac gcgcagcgat ttattgctca gatcggttgc gccagtgact ttcgctaccg
     2761 cctggatcag cgtgcggaac aggtcgatgt catagttaga gttaacgtgt tgcagcaccg
     2821 cagcaatacg ctccagaccc ataccagtat ctacagacgg tttcggcagc ggttccatcg
     2881 tgccatcggc ctggcggttg aactgcatga agacgatgtt ccagatctca atgtagcggt
     2941 cgccgtcttc ttccggactt cccggagggc cgccccaaat gtggtcgccg tgatcgtaga
     3001 agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag ttgtcagatg
     3061 cgtatggcgc acctttgtta tcgccgatac gaataatacg ttcgcgcggg atccctactt
     3121 ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc cacagacgct
     3181 ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat
     3241 cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag gtatggtgac
     3301 gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc acgcagcgtt
     3361 gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca tccttgaact
     3421 ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc agggagctgc
     3481 tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga cggatctcag
     3541 cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgct accagccgtt
     3601 gcgtgtcgtt aaccgcccgg ctggtaactg aaaagtggga ataagataag ttttcttgac
     3661 tgggaagtaa aatcccgtat gcgttcagtc ggcaaaattt cgccaaatat cctggatatc
     3721 ttccatcaga tagccacgat agagcagaaa acgctggatc ttaacttttt ctgaaaagac
     3781 agttggcaaa ggttcgccat attttcgcgt cgcctgatcg cgcgccagtg cgcaccagtc
     3841 gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg gaaatacctt tctgattcag
     3901 ttcctggcga atacgcgcag gtccataacc tttgcggcta cggctggcga taaagcgcgc
     3961 aacaaatcgg ctgtcatcaa gatagccatg ttcatggcac caggcaataa cgcgctcgta
     4021 atcttctgcc gtggcatcaa tctcttccgg gccatttttg cccataatcg gtgccgcgag
     4081 tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc agaatgcgta ccgcacgatc
     4141 caacaggcga gcatatgccg ggcgacggga tgttgattct gtcatggcat atccttacaa
     4201 cttaaaaaag caaaagggcc gcagatgcga cccttgtgta tcaaacaaga cgattaaaaa
     4261 tcttcgttag tttctgctac gccttcgctg tcatctacag agaaatccgg cgttgagttc
     4321 gggttgctca gcagcaactc acgtactttc ttctcgatct ctttcgcggt ttccgggtta
     4381 tctttcagcc aggcagtcgc attcgcttta ccctgaccga tcttctcacc tttgtagctg
     4441 taccacgcgc ctgctttctc gataagcttc tctttcacgc ccaggtcaac cagctcgccg
     4501 tagaagttga taccttcgcc gtagaggatc tggaattcag cctgtttaaa cggcgcagcg
     4561 attttgttct tcaccacttt cacgcgggtt tcgctaccca ccacgttttc gccctctttc
     4621 accgcgccga tacgacggat gtcgagacga acagaggcgt agaatttcag cgcgttacca
     4681 ccggtagtgg tttccgggtt accgaacatc acaccaattt tcatacggat ctggttgatg
     4741 aagatcagca gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat cgcctggctc
     4801 atcatacgtg ccgcaaggcc catgtgagag tcgccgattt cgccttcgat ttccgctttc
     4861 ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga acgcgccagg
     4921 gcgtcacaga tttccagtgc ctgctcgccg gtatccggct gggagcacag caggttgtcg
     4981 atatcaacgc ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc atcgataaac
     5041 gcacaggttt taccttcgcg ctgcgctgcg gcgatcacct gcaacgtcag cgtggtttta
     5101 ccggaagatt ctggtccgta gatttcgacg atacggccca tcggcagacc acctgcccca
     5161 agcgcgatat ccagtgaaag cgaaccggta gagatggttt ccacatccat ggaacggtct
     5221 tcacccaggc gcatgatgga gcctttacca aattgtttct caatctggcc cagtgctgcc
     5281 gccaacgctt tctgtttgtt ttcgtcgata gccattttta ctcctgtcat gccgggtaat
     5341 accggatagt caatatgttc tgttgaagca attatactgt atgctcatac agtatcaagt
     5401 gttttgtaga aattgttgcc acaaggtctg caatgcatac gcagtagcct gacgacgcac
     5461 cgcatcacgg tcaccgctga aacattcccg ccgggtaatg ccttcaccac gggcagtggc
     5521 aaaagcaaac cagacggtgc cgacaggttt ctcttcactg ccgccatccg gcccggcgat
     5581 accactaata gacacggcgt aatcagcacg agccgctttc agtgcgccta tagccatttc
     5641 caccacgaca ggttcgctca ccgcgccatg ctgcgccagc gtctcttcgc gtacaccgat
     5701 catttgcgct tttgcttcgt tactgtaggt gannnnnnnn nncgccacgg gctttcagcg
     5761 ccagcccaac ctgttcgctt aactgcatca gttcactgtc agtcatcaca acctctgtca
     5821 gtcaaaaatt tatgccggac aagataacac tttcgtcagg aagatggtgt tggcgttgca
     5881 attttacgag gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc
     5941 ttacaaatag ttactgtacg cgcgccagcg ccacggcttg tcctaactgc cagaccgcca
     6001 ttgcgtaatg agtgctgtgg ttataacggg taatggtgta gaagttcggc agaccgtacc
     6061 agtactggta gccggtgcca acatccagac gcagcaggct ggcttgttga tggttgccca
     6121 gcggcnnnnn nnnnncccag cggctgctgt ggcgttaaac ctgccgcggc aagctgcgaa
     6181 atgctgtacc tggttttgaa gccatttggc aagcctggag cctgaccgtt tgccatcacc
     6241 gcaacctgat cacctttcac ccagccgtgc gctttgaaat agttcgccac actaccaatg
     6301 gcatcaaccg gatcccacag gttgatatgc ccgtcgccgc tgaaatctac cgcatattgt
     6361 ttgtaagacg acggcataaa ctgtccgtag cccatcgccc cggcaaagga acctttcaga
     6421 ttgagcggat cgtcctgctc gtcgcgcgcc atcagcagga aggtttccag ctcgccagaa
     6481 aaatactccg cgcggcgtgg gtagttaaat gacagcgttg ccagcgcatc gaggatgcga
     6541 gttttcccca tcacgcgccc ccagcgggtt tcaacgccga taatcccgac gataatttcc
     6601 ggcggtacac catacacctg ccacgcgcga ttcaacgcat cttcatactg attccagaaa
     6661 accacaccgt tctgcacgtt gtccggcgta ataaattttt tgcgataacg cagccatgcg
     6721 ccgttcggac ctgatggcgg tttcaccgat gtggttggtg cctggttatc catcagccgc
     6781 agtaccgaat ccagacgttt cgcctgggag agaatttcct gcaactgctg acgatcgaaa
     6841 ccgtgtttgt tcaccatttt gtcgatgaac tgctgggcat tcggattatt ggcgaagtca
     6901 ccgcccatct gcatcacatt gtgctgcggc tcaagcagga agccgccaga cggcgttccg
     6961 gtggtcgttt cagtctcagt aggttttggc ttgctgctac aggcggcaag caacacaaaa
     7021 aggggaagca atgttacata acgacgcttg aacatgaggg gtccatttaa cagattcaac
     7081 caggggcaag tatggtaaag catcccgccc cgcacaagga agcggtagtc actgcccgat
     7141 acggacttta cataactcaa ctcattctcc tcactacctt ctttatacaa actttcaaaa
     7201 taaaatattt atctttcatt ttgcgatcaa aataacactt ttaaatcttt caatctgatt
     7261 agattagatt gccgtttggt aataaaacaa taaatactga aggagagaac aatgatagaa
     7321 accattactc atggcgcaga gtggtttatc gggctgttcc agaaaggcgg agaggtgttt
     7381 accgggatgg tgaccggcat tcttccgctg ttaattagcc tgctggttat catgaacgca
     7441 ttgattaatt ttatcggtca gcatcgtatt gaacgatttg ctcaacgttg cgccggtaac
     7501 cctgtttccc gttatctgct gctaccgtgc attggtacgt ttgtcttttg taacccgatg
     7561 accttaagtc ttggtcgctt tatgccggaa aagtacaaac ccagctatta cgcggcggcc
     7621 tcttatagct gccactcaat gaatggcctc ttcccccata tcaaccctgg cgaactgttt
     7681 gtttatcttg gcattgccag cggtctgacg acgctgaatc tgccacttgg cccgctggca
     7741 gtgagttatc tgctggttgg tttggtcacc aacttcttcc gcggctgggt gaccgatctg
     7801 accaccgcca tttttgagaa aaagatgggc attcaacttg aacaaaaagt tcacctggca
     7861 ggagcaacat catgacacgc attcggatcg aaaaaggaac gggtggctgg ggcggcccgc
     7921 ttgagctgga agccacgccg ggcaaaaaaa tcgtctatat caccgccggt acccggcctg
     7981 cgattgttga caaactggca cagcttactg gctggcaagc tatcgacgga tttaaagaag
     8041 gtgaacccgc ggaggcggaa attggtgtcg cggtaatcga ctgtggcggc acattacgct
     8101 gcggcatcta tccgaaacgg cgtattccca ccattaatat ccactcgacg ggcaagtccg
     8161 gcccgctggc gcagtacatt gtggaagata tttatgtctc tggtgtaaaa gaagaaaaca
     8221 tcactgtagt gggtgaggcg acaccacaac cctcttccgt gggccgtgac tatgacacca
     8281 gcaagaaaat caccgaacaa agcgatggtt tactggcgaa ggtgggaatg ggtatgggtt
     8341 ctgccgttgc cgtgttgttt caatctggtc gtgacaccat cgacactgta ttaaaaacca
     8401 ttctgccgtt tatggcgttc gtctcggcgc ttatcggcat cattatggct tctggccttg
     8461 gtgactggat tgcccacggt cttgctccgc tggcgagcca tccactgggt ctggtcatgc
     8521 tggcgctcat ctgctccttc ccgctgcttt cacctttcct cggcccaggc gcagttatcg
     8581 cacaggttat cggcgtattg attggcgtgc agattggtct cggcaatatt ccgccgcatc
     8641 tggctttacc tgcactgttt gccatcaacg cgcaggcggc ctgcgacttc atcccggtcg
     8701 gtttgtcgct ggcggaagcc cgtcaggaca cggttcgcgt cggtgtccct tctgttctgg
     8761 tgagccgctt tttaaccggc gcgccaactg tactgatcgc ctggtttgtc tccggtttta
     8821 tctatcaata gaggctgaaa catgaccgtt atttatcaga ccaccatcac ccgtattggc
     8881 gcgagtgcca ctgacgccct cagcgaccag atgctcatca cctttcgtga aggcgcgcct
     8941 gcggatctcg aagagtattg cttcattcat tgccacggcg agttgaaagg tgcactccat
     9001 cccggtttgc aattttctct cgggcggcat cgctatccgg tgaccgccgt tggcagcgtg
     9061 gcggaagaca accttcgcga actgggtcat gtcaccctgc gcttcgatgg tttaagcgaa
     9121 gcggaatttc cgggcactgt ccatgtggca ggccctgtac ccgacgatat cgcgccggga
     9181 tcggttttga agtttgaatc tgttaaggag taaaaaatga atcaggttgc cgttgtcatc
     9241 ggtggtgggc aaaccttagg cgcgttcctg tgccacggtc
//
LOCUS       DAWDRC010000078         9266 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3733_length_9266_cov_2.677234, whole genome shotgun sequence.
ACCESSION   DAWDRC010000078 DAWDRC010000000
VERSION     DAWDRC010000078.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9266)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9266)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3733_length_9266_cov_2.677234"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5086..5095
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2446 a         2170 c         2150 g         2490 t
ORIGIN      
        1 ccacacgctt gaccagcagt tgatagagga agcgatggcg gtaccgctac tgaaaacact
       61 catcgaggag atccgccatc tgaccaatcc gcaaatcgtg ggctgggata tttgtttttc
      121 ccgactatgt cagtcagaag tcgacagcaa cagccttgtg caactggttg cgctggaaag
      181 tgaaaactgg cccgctgact cgttccatga agcgacgctg ctccagttac tatcaagtca
      241 gattccacca gacgcgttcc cgctccttcg taacgtgatg cctgccttta ttgaatggct
      301 tgagcgtcat catttcagtc tgagtagcag gacgtggcta aagtggctgg atgttcttgc
      361 gatggaacaa agcgtcagtc aggcggatat caagctggcc acgatggcga cggagcgctt
      421 cctacaaggc tctgttagcc aggaagcata tcaacagttc ggagccatgt tggaactgat
      481 cgtcgaacgc gccagttcgt tccgtaacct gtcagtgctg ggcgagttga tcgaactctt
      541 ccttgattct ccggtgcagg atcaggcgac gctcacctcg ctgtggctca gcgttcagtc
      601 ttttgtgagc ggaatatgga cgcgactgga tccgactgct cgtaccgtga tgcgcaactt
      661 ggcggctgac gtgctagggg aaggtgcaga gagtgtattt ccggttgagc aggatagctg
      721 ttcggcagat gcagaagatg aattgccgga cttatccgga gccagagtgg cgatttatag
      781 cttgacagaa ggtgctgcaa gacgcgcgaa acggatgctg gaaacgctgt ttcccgggat
      841 cggagtggag atcagccaag cacacactgc aaccgataag ttgattaatc aagcaaaaca
      901 ggctgattac ttcatctttt cggcaggcag cgccactcat caggcttttt acgcggtctc
      961 agcgcagcgc agagatttaa tctacccgac aggtaaaggt gccgggtcaa tgctgaacgc
     1021 gtttattacg cacgttcagc aagtctcaac agtagtggta taaaaatagg ggcaaatgcc
     1081 ccctttttat atattggcgt tcgctgcttt gatataactg gtgaccagat aatccgtcac
     1141 taatgagata tctttcgaca ggttctgcag ttcattccat ttacgaacaa tgggctgacc
     1201 atcaatatcc gtaccgaagt tccacgcgcc actactccat gcacaaagtg gcttgatgcg
     1261 gtttaactct tcgacaaaac gagtatagga aggaatctca gtccagccag gttcaccttt
     1321 atagtcctga tgaacctgat cgatttcatc catcaaacta ccaagagcaa gaataccaac
     1381 gccgtgtaat aggcgggaat agcgtggctt tttgtcccaa tcatcggtaa acacggtttc
     1441 caccgccgac cagaagttat tcagcagttt caccatcttc acttcatccc ccaaacctgt
     1501 cgacgggtca cggaatcgat acaatgctcc ctcacgaaga ctggcatcga tcattttaat
     1561 catcgagttg tcagcaataa ccccatcagg attggttgcc gttttgatac gtcctgcaag
     1621 tggtccttcg ccgaagttta gtcgttgagt cagcagtgaa ggaaacttac gcatttgcag
     1681 atcggatggc aaacgaccat gggtatgtgg tgccaattcg tataacaacg ttttgggcag
     1741 aggttttgtg gagttaacca gcataaactg ctcgcgttgc tcttctgcat cattagcgat
     1801 aaatgcagag accggcatca taaacgagtc gatttccgca tcccgcaaag cagcggtacg
     1861 ttgttgacca tccacaataa acccaggctt ctcaaaatcc ttatcttcgg agaaaggtat
     1921 caccagatgc cccatgtcgc cattatctgt aagcggttca aaacgcacac gcttatcaaa
     1981 agcgataatc accgggttgg gtatcatcgg gtttttactc tcaatatagc gctgaatttc
     2041 gcgaatatgg ttacgtactt ctggacgctg ataaccaacc aaattctctt caccgcgacg
     2101 aatacgcgaa atcgctgcaa ttttgctaat ctgtttacct tctatcgcaa ggctatagag
     2161 ttgcctctcc tcaccctgtc ggattcgcag ggcgggaaca cgatattcac tcatgacagt
     2221 tcctctgagc gtaatgacaa tgtgtgctgt aaacggttgt acaacacctg aatattatgg
     2281 aaaccgcggc gacggttacg ttctgctccg cggaaaatga tgacgttgat accgatagct
     2341 tggcaaatat tgcactggca ttgtttccag ggacgttcac ccagtgtgcg ttcataatcc
     2401 gcacggattt tattggcctc tttttcacct gaatgcaggc gttcataagc cagtactgcc
     2461 tcaagagttt tttcaagggg caaagcccct ttatcgtatt cgaacaaggc attcatggca
     2521 tccttttcca gatgccgagc aatatcctga tcaatcacgc cggattttat tttgcgactc
     2581 aaacttggat tcgcatcaaa ctgaggaact cgcacagcgg tataggccgg gccatcaggc
     2641 gtatggtagt tattttttcg atctttgaag gcctgctgca gcggtgttgt cgagtcaata
     2701 ctggtcacac caaacttgat aaagtcggcg aaactctctg gacgggcaat accaagcaag
     2761 tgaacacgtg tatcactttt cagcagcggt ttaatttctt cgagggtctc aaggatctgc
     2821 gtcgttttca acggtaccat cccccccatg gtgatatttt tgtatcccat ggctagcagg
     2881 gcctctacag actgacggta ggagttttta ttccatccat gtgcaacacc aaagggagtg
     2941 aaacaggact tttgagactt gtttaaaaac tcttgtgcca gggtcagagt gatatcctgg
     3001 cgtcggcgac attcagtcaa tacttcgcca ctgaatactt cccctggcct ctcataacca
     3061 aagacgatgt gatcgagaga aacgccatag ttaaagcggg aagtttcgta gaactcaatc
     3121 acctcttcca cacggtatgg ggggacgtcc tggttaacat aggtgaaggc tccgcaatca
     3181 cccatagttt ccatattttc tggaagacgg aaaaagtgct tcattccatt acggaaataa
     3241 cgtaaacgct gggcacgggt atatttgctc tcaccgccac caagaccatc tactacggct
     3301 ttagaaacca gcataccatc gtaaggatga gaaaaaacct catgcggata atgatcgtca
     3361 cgttgacgca cgcgatgttc gttacgcgtt tcgcggaaga agtcgaaact gggatcaata
     3421 aaatcctgac tatctgggaa aaaatacttt agcttcgaca acttactctc ctgtgacacc
     3481 actgcaacgt ctcaaaaaac gagggattct accatgaaat cccagcaagt agcattaagg
     3541 tttagcctct ctaagggcct taaactcagc ccgaaaacgt ttttcctcaa atgaatttcc
     3601 cgagtcacga aattcacgca acgcatgtgt tgcactgaca ctaggatttt gttccataag
     3661 ctttaaaatg aatgacttaa cttcctcttg gctacgacga attccttttt tcacttcgcg
     3721 cacaggtaac tctttgagtt cttctgttaa cttttgagat aactcattta aatcattgag
     3781 ttgttttgac gtaaattcct tcacaatgta ttttgctaca ctgatattaa gcataagcat
     3841 attgcatctc aaccactgag ccatattttt gtttgttttg aaaaggtatg ccgttagcct
     3901 acgattgacc ttttgggaag ttataatgag cagttgtttt tttgcgtcag ccaaatactc
     3961 aataccatta ataatatcca attcaattgc attaatatag tctggagaac cgcaaataat
     4021 ataatggtct tttttctttt ttttcattaa ttctacaatg gatgtagggt gcttagatct
     4081 aaaaatagat ctattagtta tttctttcca ccatgttctg tgaaaacttt ttcctatata
     4141 ttcttgtgaa tacaatggta ttgattcatc atagcccaca gcaaaggtcg ctttatacgg
     4201 tactacattg tctttgtggt ggaggaggcc taaaccggca gaaagaaccc ataaatcaat
     4261 agggtattca tttaagatcg ctttagctgt cgcccagtgc ccacctttat aaagatcttc
     4321 tgcacataca gttgcagatg tagatatcgc ttcattcaga gcattgcacc aagaattcac
     4381 gagcacattt gaagggggcc ggcctgatga aaattgactc agactcaata tatccccact
     4441 atcgtagtgt ttaccgtttg tacatgttgt tattaagttg atattgctca caatcgcccc
     4501 cctaaatcgt gctacacaag ctacaggaga aaaaagggga tgacaagaac ggaattatca
     4561 gtccctgaaa aatttttcag ggactgatgc tagtgaggaa tatactcgaa atactcatct
     4621 tgagaatgag aacacttttg gcatcgcctc agaatacctt tttcctcaag agagagacaa
     4681 aaattgcgac atgattcaag aaggtcgtca ggattttcat ttttattttt agaaatcaag
     4741 gctctagcga gtgtgtcata actaaaacct cccgttgagt gcgatacaat gctcatcgca
     4801 agttgggtat cgaaagtttt catagtgcac ctctgagccg ccagaataat acagttataa
     4861 aaaagtatgg gttatagttc aagctggctt ttcaaattcg aatatagcaa gcatcaggaa
     4921 ttagactaca tgccaaagtc ataaggacta acttcccttt tcctattcac atccaaaaat
     4981 cagcccacta ctgcagctca gtcgccgttc atcaatatgt tcggccttat gaacgtaaga
     5041 tgtacgtact gagttacgtt ctgatcaccg ttcagcacca tgtccnnnnn nnnnntggtg
     5101 acggttttac ctttgtcgag ggtcgtttcg gtccattcag caccgttgcc ttttgctgca
     5161 ttaacactag caaagacgtt gataccggca ccgacgccag cccgtcttgg ttgttctatg
     5221 tcgattctat ctggcgtcag ggggattata taaaccagta tggtcccggc acgccggtgc
     5281 agcagtggat gactactgtg atgccgagac atacagctct attgtaatat aagggcttcc
     5341 ccggcatgtc aacgccggtg tttatggttt ctgtctgctc ttcagtaaaa ataaagggga
     5401 gagacaacag aacgataaat ggccatgatg agtcgtcatc gactcggacc ttgatgaaag
     5461 acgcgcgtga ggttcttctt cgttaagcgg atacgtatat tctgtacaat agccagatag
     5521 gcctcacggg gtctaaccct ttatcatcaa cgaattgacg gtcacatggg tattttgttt
     5581 tgtgctttag ctggctgagt acagcctgaa attatcactg taatgcaatg atgcccagca
     5641 cagccttgcg tttttggcgg tgatctctac aacggtacgc cagtatcctc tgcgctcgac
     5701 cagtgatcag ccccaacgac tgaatgaatc agtccttttt tcggcaccaa tcagaaccga
     5761 acgcccccct gaaccagcag aattcgcaga tacgtatcgc cagctttagt tatcctgcca
     5821 agcgttgatt tcccactact gctgtattgc gatagtgtta gtcccagcca agttgccagt
     5881 tgtcgctcat tttaaaatcg tgtgcatttc cgataatggc gaccagcgca caggccgttg
     5941 tggtactaat ccctttcagt tccattagtc gctgactgcg gtgatctgct tttgccatgc
     6001 gggacaaaac cggtcatatt cagtaatatt atcttcaata cgatcaatgt gtaccagcag
     6061 gtcatgaacg tattgttgaa cctgaagtgg caaaaaactc ttctgctcag aaaacatatg
     6121 acgaagggca tctgtacttt gtggggcgat gacactgaat tccgatatca aacctctcaa
     6181 tcggttatat gttgctgttt tctcttcgat aaaaccctgt ctggtacggt gtaggcattg
     6241 catcgcctgc tggttctcat ccttcactgt tacaaaccgc atatgtgggc gacgaactgc
     6301 ttcacagata gcctgagcat tatttgcgtc atttttgcct gatttaccag ccatgcgata
     6361 aggagataca aatttagcaa ccatcagacg tggttcatga ccatattgtc gaaacaacct
     6421 tgtccagtag tgagccccgg agtatacctc catcccgata acacagagag gtaaactggc
     6481 aatcagctaa ggaatggtag tatccgatat tttggggttt aaccagaata gtttttaccg
     6541 tgttagtcaa cgcagtgagc agcgaacaca tttttaacaa gatcgatacc gacagtcgtg
     6601 atggtcataa cgaatccctc cggggctatg tttaccccat gattgcataa gagttaatca
     6661 ggcgcatatc ttggggaagc ccttttcatt cgtaaaggcc cgctgttccc gctgaattcg
     6721 ctgatgtacc atgggatagc cagcgccgag aatgcctatt atggactaga aaaagagcaa
     6781 acggaccgcg attttttcga ttaggtctgg acctacttcg tgaccagcac ctaactgcag
     6841 gagctgtata ttaccccatc tgtgctgaaa aaggtgaagt gggatacgtt ggcgaaggtc
     6901 gcaaaatggt tgaaggaaaa tgccagcgtg ctggttgata cccactgggt tggtggtgac
     6961 tcgacggcgc ttgccatgta cgactggcca tcttggaaca aagacaaagc cattcttggt
     7021 ttgcgcaacc cgtcggataa gccacaaacc tactacctag agttagcgaa ggatttcgaa
     7081 ataccaacag gatacgcggc gcaggttagt ctgaaagcgg tatatggcat taataaaacc
     7141 ttgcccgttg agtataaaaa cgcaacagtg attacgttgc aggcactgga aacgctggtg
     7201 tttgaggcga tacccgttaa ctaaacgcct gtacctatga gtaggccggc taaggcacag
     7261 cctactcggc aagaccagct acagggattt actccccctg ctgcgcaagc gcctttttca
     7321 ttggcaattc ggcctatagc tacgcgtcgg caggcagacc aacgaggcgc tcaaggcggc
     7381 aattgccgca catgactcag gtagcggcat tatacagttg cctagatatc cagatacccg
     7441 cttcgtgaaa agtacgtatc agatgatagc ttggatcgtt aataacgata tcaggaatga
     7501 agtctcactg ctattgctgc cggtgaggat gtcgttcctg cttcagagtt ggcaactgct
     7561 ataaaacaaa taaaagatgg ttacctatgc agggttccag aatacttggt tctgacatat
     7621 taaatctctc ggatgggatt ttatcggtcg tatcagaggt aacattcagc tacgtattga
     7681 aagtaaagga caatactggt tcaggtgtca gaagttgctg gccagtagta aaccggaata
     7741 tctgggccgg gaacgcttgc cagctcagaa tatgcgcggt gcaatggttg tttttatttg
     7801 caataaaaag agcagaaagg ccggaacaat aaacgtgccc acagcggtat agccagacca
     7861 tcacaaatac aagatgccag ttcggcggca aaagaaccgt ggttaatctt tagtagcata
     7921 aaaggcttta agtctagtta aatcatgttg ttatacagtt acagaatgaa gattgagcaa
     7981 aacttccgga atgaaaaggt gagcgttttg gtttcggcgt gcgtgccagg tacagtcgtt
     8041 cggctggaaa aatacggggg ctgagcctgc tggcaacact gagcacaata gtgcggtggc
     8101 ttattggcta tcacgcttaa aataaagggg tagatctgag acagcaggcc aacagaatta
     8161 aaacgcggcg ggtaatatcg tatctgacat tagcgaagaa tatcttgcga tactcctcgc
     8221 taattttaaa acgaatggtg ctgatcaccg ttcttaatca cttcgccaga accgaccata
     8281 atatggtgtt ggtttattag cgctgattca tggggatccc tcagtgcttt tgcggttttt
     8341 tatatcttta aagcagtagc ggtagggtcg cgctggcagt ttgctggctc acatcgctgt
     8401 acttatgatc cagcgcaatc agcgatgtgg tgagtaactc taccaacagc ataactccca
     8461 ccttggcatt cagcgcgccg gcgctcaagg ggccttccgg tttggccgcg accagttgaa
     8521 tatcactcag cgaagctaac ggactgcgcg gcgtattact cagcgccagt acacgcacac
     8581 cttgcttacg cgctagtttt acgacatgca gcagatcgcg cgtggatcca gaactggaaa
     8641 tcgccaccac gaacgtgttt ttactaagtg ttgtggcgtt catggcagca cgatgcatat
     8701 cgctgaacaa ttgtgcgggt ttccccagac gcaggagttt gtaatgtaaa tactccccca
     8761 gaatagcact ggctgctacg ccataaatct gtactgactg agcctgatgc agtgcaagcg
     8821 tggctgcttc cagcaaggtc ctgtcgagta attttgctgt gtcctgtagt gcttgcacgg
     8881 attcattgac cacattgtca atctcatcac cgccgtgctc tacagactgt acttgtcgga
     8941 gatcgagtgc aagcgccatt ttgaattcgt tatagccctt acaccccagc gttcggcaca
     9001 gacgtgtgac gctagcctcg ctggtgccgc tttcacgcgc cagttcggta atcgtcaggt
     9061 agacaacttt tgccggatcg ctaagtacaa actctcccag cttctgttga gttcgactat
     9121 atccatcaac gctctggcgc aatctcagca gcaggttttc attttctggc atgcaaaatc
     9181 cttaatgcgt ttctctgccg atagtgtggc ggaaagtagg ggagagatgc cagtcatttt
     9241 aaaaaagtat gatcggtgtc ttggtt
//
LOCUS       DAWDRC010000079         9256 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3740_length_9256_cov_2.573525, whole genome shotgun sequence.
ACCESSION   DAWDRC010000079 DAWDRC010000000
VERSION     DAWDRC010000079.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3740_length_9256_cov_2.573525"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1078..1087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3755..3764
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7128..7137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2296 a         2371 c         2224 g         2335 t
ORIGIN      
        1 gtacacaggc gctattattt aattcgcgat taactgttca tttattttgt cgataatatt
       61 gattgaggga tctgacgcta cgacgcgtaa atccagtatg acgccaagat tatttaactc
      121 atgaaatgcg ttaatatcat cgtcatctaa tgaaactgct ttggttaact gtttcttacc
      181 aggacgccag gccataccgc caatattcag ggtacctatt tttaccccct ggcgaaccat
      241 tgctaaggca tcctgtgggc gagtgaaaag ataaaaaacg gtttcgtcct gatattgcgg
      301 gttgtgataa accgcgacgg ctttttcaat attgaccacg ttaaccttca ttcccggtgg
      361 cgcagcctga cgcaataatg tccggcgaac ttcatcgtta taaacttcgt cattgcagat
      421 aataatgcgt tgggcgttag ccactttaga ccagacggta gtgacctggc cgtggatcag
      481 gcggtcatca atgcgggcga gcgtaatgtt catcagaaat cctcctccgc tgttgccacc
      541 tgtctccaga cgacgcaggt ttgctttgcg atttgcgtca ggtgttcaca cagttcatca
      601 atattcatgc tgtcctggtt atcaaccagt tcgagcgcca gcggcagaga aaggccgctg
      661 attacccgca gacgaggatt gcgcatcgca agcatcgctg ccgcattcca cgggctgccg
      721 cactgtaaat ccacggcgat tagccactca tcttgattgt gaatgcttac taacttctcc
      781 agctttgcca caatgtctcc ggcgttctcg ccgggcacaa attcaactgc ctcgaccctg
      841 gcatcgccgt aaaccatctg aaccgattcc agcatggcgc aagctaattt gccatgcgca
      901 caaaaaatgg cattgaccat aaagcctcct tagccgcgcg ttttgccgcc cgcaatgtta
      961 gtggttactc cggtgatata gctggcgcgt tcagacagca gataacaaac aaaatcagcc
     1021 acttctgcta atcttccggc gcgcccaata ggaatggcgt ttttggtata gccttcannn
     1081 nnnnnnnctg ctcgacggtg atattgcgcg tccacgccag cgcctcttca tattccggcg
     1141 tgcgcagccc tgttttttcc agaatccccg gcgcgatacc gaccacacgg ataccgtgct
     1201 tacccagctc tttcgaccag gagcgcgtga agctattgag cgcggctttg gtcgcggcgt
     1261 aacagctttg gccttctgag ccttccagcc cactttctga ggaaacattc acaatcacgc
     1321 catcatgttg tttgaccatc tgtcgcgcca ccgcctgcga catcagaaaa acgcctttct
     1381 ggttgatatt gaccattttt tcgaatgcag cttcgttgag ttcatactgc ccggcaggcg
     1441 ctttctcatc gacaagcaga cgcgggaaat tgaccccggc gttgttaacc agaccgtcga
     1501 tgcgaccaaa acgctggata atttctgcta ccgtatgatt tatctctttg gcgctggaaa
     1561 tatcggtcgg ccagaactga taacctttat ggctttcata ttgaccatcg ccaccgtgaa
     1621 tatcgaccat ctgtacattc gcgccttgtg ctaataattc ctctacaatc gccagaccaa
     1681 taccggatgc gccaccggtg acaataatga ttttatcctg caaatttaac cacgtttgca
     1741 taacagactc ctgattttag ataataggga cccgccgtaa tttttattac ggtaaatatc
     1801 gggaagattt ttatttcagt aatagttcag ctgtgctgct attcgttacc agacaattaa
     1861 tatattttcc acgcagtgca ccaacaattc cactgtattt ttcttcgctc atggcaatgc
     1921 caatggaata ccgtgcctgc tttaatttat tcatttcgat agagagtgtt ttttcgctca
     1981 tattcgtttc aaccattgcg ccgtgaatat caaaaaagcg cgaacaaata tcgccagcaa
     2041 cctggcgggc attcaggtcg tcactctctt caccaccata aaacgcatgc cagttagcgc
     2101 cgtcgcgaat ggccggtgag ccaattccca ccagggcgac atccagatta tcccagtagg
     2161 cagagatggt tttaaagtgc tgagactgca tgatcccatt acgaattaat gggttatcca
     2221 gcagagccgg aaaatccgcg agatgcgatt cccctttcag cttcgctgcc gcgctgtagg
     2281 ttaatgtgtt tacgtgatag cggctttcga gtttaccgga cgggccacca ataatcggca
     2341 cgcaaattaa ctgccgcgat tgccccgcct gcggcaagtt ttcaaccagt gcgctcaccg
     2401 cgcggcccca ggaaaaaccg acaatatcgc caggttccag caagcgatcc agcagttgcg
     2461 cgccgtgtaa ccccatcatc gccagttgag tatcttcatc ctcatcattt cctgacacca
     2521 ccacaacgtc tttcagacca aacttttgct tcacttgctg ctccagccag agattttcgt
     2581 tgtagtcata gttgatggcg atggtgacaa ttccctgatc gcggcctcgt ttaagcaagc
     2641 ggctgatggt ggtgcggtaa atacccagtt cgcgcgcaat ttgcgcctgc gtcatatcct
     2701 gttcgtaata gagttgggca atcttcacaa tcaaacggat atcgtcactg ttttccattg
     2761 ccgcctcctg gttctgcaca tttgtgcaaa tacgctttct caccgcttga tctgatataa
     2821 ccccatcatg atggcctttc aaagcgattt ttcactcttt gcatgggggg aaatagcgct
     2881 ttctcttgca catttgtttc tctgcgtagt agtcatcagc acaggtgtgc agcgatgcgt
     2941 aatttgttga ttattcttat tttgccaggc aaatttgcat cgtacttccg gagacacttt
     3001 tgtgataaaa ggcgtggcag atcacattta atttcgacgt tgccgcataa ccctgttttt
     3061 ccccgctaag aggtacggta tattccgcgt tttacaggag aatttatgct gcccgactca
     3121 tcagtccgtt taaataaata catcagcgaa agcggaattt gctcacgccg cgaagcggat
     3181 cgctatatcg agcaaggcaa tgtgttcctt aatggcaagc gagccaccat tggcgatcag
     3241 gtgaaacccg gcgacgttgt gaaagtaaat ggtcagttga ttgagcctcg ggaatccgaa
     3301 gatttggtac ttatcgccct gaacaagccc gtcggtattg tcagcaccac cgaagatggc
     3361 gagcgcgata acattgtcga tttcgttaac cacagcaaac gcgtgttccc gattggccgc
     3421 ctggataaag attcccaggg gctgattttc ctcaccaatc acggcgatct ggtgaataag
     3481 atcctccgtg cgggtaacga tcatgagaaa gagtatctgg tgacggttga taaaccgatt
     3541 accgatgaat ttattcgtgg tatgggggcg ggagtaccga tcctcgggac ggtaactaaa
     3601 aagtgcaaag tcaaaaaaga agcaccgttt gtcttccgca ttaccctggt gcagggtctg
     3661 aaccgtcaga ttcgtcgcat gtgcgagcac tttggttacg aagtgaaaaa gctggaacgt
     3721 acgcgcatta tgaacgtcag cttaagcggc attcnnnnnn nnnntgggtg agtggcgcga
     3781 tttaaccgac gatgagttaa tcgatctctt taagctcatt gaaaattcct cttccgaggc
     3841 aaaacctaaa gcgaaggcca aaccgaaaac ggtaggcatc aaacgtccag tcgttaagat
     3901 ggaaaaaacg gcggaaaaag gcggtcgccc ggcgtccaac ggtaagcgtt ttacctcgcc
     3961 ggggcgcaaa aagaaggggc gttaatcagc gtctgccgcg cgtcgacgta tttgctgacc
     4021 agctaaatga cgcctcagga tccggtttat aagcctgctt tttcttcaat tggcgggctt
     4081 ttttcgcttc agcctcacgc tcgaccatta atttatcgat gtactctttc ttaatgctgt
     4141 tagtttcgga gttggtcagt acgcgaccat gcgcaatccg cgcccgatcc agcagtgttt
     4201 taagttcacg ctgctcgcgt tcagtcatct ctttttgggt aatacggggg agtgccataa
     4261 tggtgccctc agtgagccag agagttcagt gtacgggaat tcgtttgcga gcagaactga
     4321 taatattcat tttgccttct cttaactatt tatctctttc gataattagt tagagatgcg
     4381 gataaaaaat taaaggcaat tattctccga taaagatgct ggttaacagg gaatagactc
     4441 gtttatttat gggcataatg acaagaaaaa caatacggcc cgaaataatg tcttccgggc
     4501 catactgtgt ttactcaaac aaattaaaat gtagcttctg caccacctgc tcggcatctt
     4561 cgccgggcac caggaagcac aggttatggc tggatgcgcc gtaacaaatc atgcgaatgt
     4621 tgaacggttc cagtacgccg aatacctctt tgccaacgcc gcaggctttt gacaggtcat
     4681 tgccaatcaa cgcgaccagc gccagaccct cttccacttc cacccggcac agtgcagaca
     4741 gctccatcag cagagattgc gtcagcaacg tatcgccagt ggaggttgaa ccggtggtat
     4801 caagggttaa tgccacactc acttctgacg tggtgattaa gtctaccgaa atattatgcc
     4861 gcgcgaggat gccgaaaact tccgcgagga aaccgcgaga atgcagcata ttcaggctgt
     4921 gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc gcggaacagc ggcggatttt
     4981 cagttttatt gcacaccagc gtaccacctg cgcgtgggtc tttgctggag ccgacaaaga
     5041 ctgggatatc gctgcgtact gcgggtagca acgttgccgg atgcagtact tttgcaccaa
     5101 aagttgccat ctctgccgct tcggcaaacg cgatttcatc aatgcgtttt gctgcggaaa
     5161 ccacgcgtgg atcggtggtg tagatgcccg ggacgtcggt ccagatatca acacgagatg
     5221 cgtgtaaagc ctccgccagc aaggctgccg tataatcgct gcctccacgg ccaagcgtcg
     5281 ttgtacgacc tttattttcg ctaccgataa atccctgggt gatcactaag ccttcattga
     5341 ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc ggctacatct ggctctgcac
     5401 gaccaaatcg gtcgttggta cgcatcactt tacgtacatc aaaccactgt gcctgaacat
     5461 cgcgttcgcg caggatctcg acaaacagca gggtcgacat cagctcgccg tggctgacca
     5521 gttcatctgt cagggccgga gacgttgcca gcgccgccgc ttctgccaga acagtaatat
     5581 tctccagcag acgttcaatc tcttcacgga taacgttcgg gtaacgcaga cgttccagaa
     5641 tggcaaactg gatattgcgg attgcgtcga gtttttcgaa tcgctcgcca ggttccagtc
     5701 cttcagctaa agcgaccagt agattagtga taccagcaga agccgagagg acaactaaac
     5761 gtacgttggc atcagaaagc acaatatcag cgctgcggtt catggcgtca aaatcagcta
     5821 cgctggtacc gccaaatttg gagacaacaa tttcagacat aactacctcg tgtcagggga
     5881 tccattttca gccttggcac aagggaagag cggaagacgg gtgggcgcag agcgatactt
     5941 cgctactatt ttcacccaga agtgctccac cacttgcgaa acgcccgact gcgaacgctt
     6001 ctggtgacaa cccaggggat tcagcccctg tagccgatga tgaacgtagc cagccgttca
     6061 atcacctcgg cgatgcaccc cctcaggtgt tatcacagga ctggctcctc caacaccgtt
     6121 acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg tagtacattt ataaataaag
     6181 ggtgagcggg gcggttgtca acgatggggt catgcggatt tttcatccac tcctggcggt
     6241 cagtagttca gctaataaat gcttcgctgc gctaagggtt tacactcaac attacgctaa
     6301 cggcactaaa accatcacat ttttctgtga ctggcgctac aatcttccaa agtcacaatt
     6361 ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc caacgcagac cgctgcctgg
     6421 caggcactac agaaacactt cgatgaaatg aaagacgtta cgatcgccga tctttttgct
     6481 aaagacggcg atcgtttttc taagttctcc gcaaccttcg acgatcagat gctggtggat
     6541 tactccaaaa accgcatcac tgaagagacg ctggcgaaat tacaagatct ggcgaaagag
     6601 tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg agaagatcaa ccgcactgaa
     6661 aaccgcgccg tactgcacgt agcgctgcgt aaccgtagca ataccccgat tctggttgat
     6721 ggcaaagatg ttatgccgga agtcaacgcg gtgctggaga agatgaaaac cttctcggaa
     6781 gcgattattt ccggtgagtg gaaaggctat accggcaaag caatcactga cgtagtgaac
     6841 atcgggatcg gcggttctga cctcggcccg tacatggtga ctgaagcact gcgtccatac
     6901 aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg ggactcacat cgcggaagtg
     6961 ctgaaaaaag taaacccgga aaccacgctg ttcctggtag catctaaaac cttcaccact
     7021 caggaaacca tgaccaacgc ccacagcgcg cgtgactggt tcctgaaagc ggcaggtgat
     7081 gagaagcacg ttgcgaaaca ctttgcggcg ctctcaacca acgccaannn nnnnnnnccg
     7141 ttggcgagtt tggtattgat actgccaaca tgttcgagtt ctgggactgg gtcggcggtc
     7201 gttactcttt gtggtcagcg attggcctgt cgattgttct ctccatcggc tttgataact
     7261 tcgttgaact gctttctggc gcacacgcga tggacaagca tttctccacc acgcctgccg
     7321 agaaaaacct gcctgtactg ctggcgctga ttggcatctg gtacaacaat ttctttggcg
     7381 cggaaactga agcgattctg ccgtatgacc agtatatgca ccgttttgcg gcgtacttcc
     7441 agcagggcaa tatggaatcc aacggtaagt atgttgaccg taacggtaag gttgtggatt
     7501 accagactgg cccgattatc tggggtgaac ctggcacgaa cggtcagcac gcgttctatc
     7561 agctgatcca ccagggaacc aaaatggtac cgtgcgattt catcgctccg gctatcaccc
     7621 ataacccgct ctctgatcat caccagaaac tgctgtctaa cttctttgct cagaccgaag
     7681 cgctggcgtt tggtaaatcc cgcgaagtgg ttgagcagga atatcgcgat cagggtaaag
     7741 atccggcaac gcttgactac gtggtgccgt tcaaagtgtt cgaaggtaac cgcccgacca
     7801 attccatcct gctgcgtgaa atcactccgt tcagcctggg ggcgttgatt gcgctgtatg
     7861 agcacaaaat ctttactcag ggcgtgatcc tgaacatctt caccttcgac cagtggggcg
     7921 tggagctggg taaacaactg gcgaaccgta ttctgccaga gcttaaagat gacaaagaaa
     7981 tcagcagcca cgatagctcg accaatggtc tgattaaccg ctataaagcg tggcgcggtt
     8041 aatcattgtc gatatgtagg ccggataagg cgttcacgcc gcatccggca accgatgcct
     8101 gatgcgacgc ggtcgcgtct tatcaggcct acaggtcgat gccgatatgt acatcgtatt
     8161 cggcaattaa tacatagtac gattgattaa atagccttaa aaacaatgcc gactataagt
     8221 cggcattgtt ttatcagata aatcctcttg tctgtcattt aacgaaaatc ataccgtgag
     8281 gttaatccta aaataaattt ttaatcgtgg tttatttcgg aaaatacgca gagtaattac
     8341 tttttatttt attttaactt catgattttt aatcttattt atatataagt caaagcttat
     8401 atttgatatt cattccaatt gtcctaaaac tccatcacta attccccgcg ccgtaattcg
     8461 catgctttag ttgtgtatac tcgatcccgc ccgaaatgtt ttgggtaaat ctccattcat
     8521 tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct
     8581 tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag gatcggcagc
     8641 aacgtcggtt tcggcgggta gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt
     8701 gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccaccacaac
     8761 cacgaccacc agtacccagt aataaagtat gtatccccaa aataattcga gtcatcgcat
     8821 ctgaagcttg aagtatgaag gggattaacc ataaccacac tccggtgtgg ttattctgcc
     8881 cctctggaga agagtcgtga agcgacctgc actcattctt atctgcctgc tattacaggc
     8941 ctgttcagcc acgactaaag agctgggcaa ttcactgtgg gacagtctgt tcggcacgcc
     9001 aggcgtacag ctgacggacg atgatattca aaatatgccc tacgccagcc agtacatgca
     9061 gcttaatggc gggccgcagt tatttgtggt gctggccttc gctgaagacg gacaacaaaa
     9121 atgggtcact caggatcagg ccactctcgt cacacaacat ggtcgtctgg tgaagacttt
     9181 gcttggtggc gacaacctga ttgaagtgaa taaccttgcc actgacccgc tgattaaacc
     9241 cgcacaaatt gttgat
//
LOCUS       DAWDRC010000080         9225 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3764_length_9225_cov_2.169575, whole genome shotgun sequence.
ACCESSION   DAWDRC010000080 DAWDRC010000000
VERSION     DAWDRC010000080.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9225)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3764_length_9225_cov_2.169575"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3205..3214
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3474..3483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4680..4689
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7857..7877
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2235 a         2440 c         2247 g         2252 t
ORIGIN      
        1 gagaacggac ccgcgatcgc cagcagggct ttcacaccgc cgttagcttc agccgggaag
       61 aatttgaact ctttcaaacc gtagtccata cccagcatca gttcggaaac agtgctgatc
      121 cccggaatca gaggaatagt cccttcggta gcagctttca gcagcggctc ggtcagaccc
      181 gggctaattg caaactgtgc acccgcttca gtgacttctg ccagctgctg tggattcagc
      241 accgtaccgg cacccacaat cgcttcaggc acttctttgg cgatagcacg gatagcgtca
      301 actgcgcact cggtacgcag agtcacttcc agaacgcgca ccccaccagc aaccaacgct
      361 tttgccatcg gcaccgcgtg ttccagtttt tttaccacga taaccggtac aaccgggccg
      421 gtggtcagga ttgattctgc acttgttttc cagtttttca tcagagtttt ctctcgcctg
      481 attacaaatt tgtcgtctta aaaagtgata caggttgcgc cctgttcggc accggacagt
      541 ttttcacgca aggcgctgaa taattcacgt cctgttccca cgcgtgacgc gctcaggtca
      601 ggaatgtgcg gttcgcgagc agccagttcc gcttcgtcta ccagcagcgt cagttcgcct
      661 gtctgtccat tcacacgaat gatgtccccg tcgcgcactt ttgccagcag cccgccatcg
      721 taggcttctg gtgttacgtg gatagctgac ggcactttac ctgaagcgcc ggagagtcgt
      781 ccatcggtaa ctaacgcaat tttgaaacac cggtccaata atacaccaag tggcggcatg
      841 agtttatgta attctggcat tccgttcgct tttggcccct gatgacggac aacaacgaca
      901 caatcgcggt ccagtaaacc cgcttcaaag gccggcataa cgtcatgctg gctttcaaaa
      961 acaaccgctg gcgcttcaat cacctggttc tctaccggca cggcagaggt tttcataacc
     1021 gctcgcccca ggttaccgct taacactttt gtcccaccat gatgagagaa aggttgttcg
     1081 aaggacgcga tcacattgtt gtcgagtgat ttttctgccc cttcccgcca gtccagttca
     1141 ccattattca gccatggttc aagggtataa cgagacagac caaagcctgc caccgtattg
     1201 acatcttcat gcagcaggcc tgctttgagc agttcacgta ccagaaccgg tacgccacct
     1261 gccgcctgga agtggttaat atcggccgga ccgttcgggt agagacgtgc catcagcggt
     1321 acaacatcag aaaggtcaga gaagtcatcc cagttaatct gaataccggc tgcgcgcgcc
     1381 atcgccacca ggtgcatggt gtggttagtg gaaccaccgg tcgccagcag tgcaacgata
     1441 ccgttcacca ccactttctc atcgatcatc ttaccgatcg gcatccattc attaccatta
     1501 ccggtcatgc gcgtaacctg gcgcgcagct gcagcggtca aagcatcgcg cagcggagaa
     1561 tccggatgaa caaaagaaga gcctggcaac tgcatcccca taaactccac caccatctgg
     1621 ttggtgttgg cagtaccgta gaaagtacat gttcccggcg catgataaga cgcggcttct
     1681 gactccagta gtgccatgcg gtccacttta ccttcggcat aaagctggcg aatacgcact
     1741 ttttctttat ttggcaaacc acttgccatc ggtccagacg gcacaaacac cgcaggcaaa
     1801 tgaccaaacg acagggctgc catggtcaga cctgggacaa tcttgtcgca cacaccgaga
     1861 aacagagcac catcaaacat gttatgggac agccccaccg ccgcagacat cgcaatcact
     1921 tcgcggctta acagcgacaa ttccattcca tcctgcccct gggtgacacc atcacacatc
     1981 gccggaacac cgcccgcaac ctgaccaacc gcattcgctt catgcagggc tttacgaatg
     2041 atttccggat agtgttcata aggctggtga gcggagagca tgtcgttata ggaggtgatg
     2101 atggcgatat tgttacgcaa catgcttttc aaagaggctt tgtcttctgg ctggcaggca
     2161 gcgaaaccgt gtgccaggtt accgcatgcc aactgcgaac gatgaacggt cgaagttttc
     2221 gcttgttcta tccgggcgag gtaagcagag cgagtctcgc gcgaacgttc aatgattcga
     2281 tttgttacgc gtaacaattg tggattcata aaggctcctg aaattgagtt gtcagagcag
     2341 gatgattcac aacacgtttc attcagagga ttcatgactg aaacgcctgt aaccggagct
     2401 catagggcaa aaacgtttca gtcagtgtaa taaaaaaagc ctcgtgggtg aatccgcacg
     2461 aggcctgaaa gtgtaaaaat tgttctacaa tctgtgcaag atcatgttac cggtaaaata
     2521 accataaagg ataagcccag atattactca aactcattcc aggaacgacc gtcacgggta
     2581 atcatcgcca ccgaggcaac ggggccccag gttccggcct gatacggttt cggcgcatca
     2641 ttgtccatcg cccacgcctc agtaatggag tctacccatt tccaggcttc ttccacttca
     2701 tcacgacgta caaacagtgc ctgaatacca cgcatggttt ccagcagcag acgttcatag
     2761 gcatctgcca gatgcgtttg attaaaggtt tctgaatagc tcagatccag cttggtgatt
     2821 tgcaggttat gtttgtggtc aaggccagga actttattca gtacctggat atccacgcct
     2881 tcatcaggtt gcagacggat agtcagttta ttctgcggca gatcctgcca cgactcttta
     2941 aacagattca gttcaggtgt tttgaaatag accacgactt cagaacattt ggtcggcaga
     3001 cgtttaccag tacgcaggta gaatggcaca ccggcccagc gccagttatc aatgtcgacg
     3061 cggatcgcca cgaaggtttc tgtattgctg ctcttgttcg cgccctcttc ttccagatag
     3121 cccggcactt ttttgccctg agcgaagccc gcagtatatt gcccgcgtac ggttttttcg
     3181 cgcacgttgg agcggtcgat gcggnnnnnn nnnncaggtc agacggcgga gacatcgcaa
     3241 tcatgcaaag aatttgcagc aggtggttct ggatcatgtc gcgcatctga ccggctttat
     3301 caaaatagcc ccagcgccct tcgatcccca cttcttctgc cacggtaatc tcaacatgat
     3361 caatggtgcg attgtcccag ttgttcacaa acagagagtt agcaaaacgc agcgccaaca
     3421 ggttcagcac tgtttcttta ccaagatagt ggtcgatacg gtaaaccggg cacnnnnnnn
     3481 nnncgaagta ttcgccaacc tgatcgttga tttcctgcga ggtcgccagc gacgtcccca
     3541 gcggtttctc catgactacg cgtgccggtt tagcattcag ttttgcctca ccaagccctt
     3601 tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta gttaatggtg atacgatttt
     3661 tttgatccag catcgcgccg agacggctga atgcagcagt gtcattgaca tcaagattac
     3721 aaaaatccag acgtgcactc agggtgtccc ataaaccttc atcaatggtt tctttcatga
     3781 aagtttcgag cgcctcgcgg acaactttgg tataagccgc tttatcccag tcagcacgcc
     3841 ctacgccgat aatccgggtg tccgggttga gctgaccggc tttttccagt tgatacaggg
     3901 aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc aggtcacagg
     3961 cctgggctgt ttgcgttacc gccatgtcat tctccttaag ttaactaacc cggtacttaa
     4021 gccagggtat acttgtaatt ttcttacggt gcactgtact gcttttacga gcttgcgaaa
     4081 actgtaaacg cttatccacc cgtgcgatta cggtaaaagc gcgcaaagtg cggcaaaact
     4141 gataaaaaaa tcatcgtttt ccattgggta aaaatctgac actgatcatg ttatgaaaaa
     4201 aaataacaac ttttttatct gcttttgtca ttaacacagc acacaggcgt aatatctgac
     4261 aaaactgcat ttcgatttct ttcagagcgg aaatcgtcat tacccgtgag tctctttaca
     4321 tcatgaatat gctggaaaaa atccagtctc agctggaaca tttgagcaaa tctgagcgca
     4381 aagttgccga ggtcattctg gcttcgcccg ataacgcgat ccattcgagt attgctgctc
     4441 tggcgctgga agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc agcatggaca
     4501 cacgcggttt tcctgatttt aaacttcatc tggcacaaag tctggcgaat ggcactccct
     4561 atgttaatcg caacgtcaat gaagatgaca gcgttgaatc atacacgggg aaaatatttg
     4621 agtccgcaat ggcaacgctc gatcatgtcc gtcattcact ggatacatct gccatcaacn
     4681 nnnnnnnnna ttaggttctt cagccgccgt tgcccacgat gcgatgaata agttctttcg
     4741 ttttaatgtt ccggtggtgt actccgatga tatcgtgctg caacgcatga gttgtatgaa
     4801 ttgtagcgac ggagacgtgg tggtgctgat ttctcacacg ggaagaacaa aaaatctggt
     4861 cgagctggcg cagctggcac gcgaaaacga cgctatggta attgccctca cctctgcggg
     4921 taccccgctc gcccgggaag caacgctggc aattaccctc gacgtaccgg aagatactga
     4981 catttatatg cccatggttt ctcgacttgc acagctgacc gtgatagatg tgctggcgac
     5041 aggatttact ttgcggcgcg gtacaaaatt cagagataac ttgaagcggg tcaaggaagc
     5101 gctgaaggaa tcgcgttttg ataagcagtt acttaattta agtgacgatc gctaaaaacg
     5161 actgtcactg tcctaatctt atacgacatc cgaatgagat taatttatcg ccatcgcggc
     5221 gttatttcat tcggatttca tgttcaagca acacctggtt gtttcagtca acggagtatt
     5281 acatgtccag aaggcttcgc agaactaaaa tcgttaccac gttaggccca gcaacagatc
     5341 gcgataataa ccttgaaaaa gttatcgcgg cgggtgccaa cgttgtacgt atgaattttt
     5401 ctcacggctc gcctgaagat cacaaaatgc gcgcggataa agttcgtgaa attgccgcaa
     5461 aactggggcg tcatgtggct attctgggtg acctccaggg gcccaaaatc cgtgtatcca
     5521 cctttaaaga aggcaaagtt ttcctcaata ttggggataa attcctgctc gacgccaacc
     5581 tgggtaaagg tgaaggcgac aaagaaaaag tcggtatcga ctacaaaggc ctgcctgctg
     5641 acgttgtgcc tggtgacatc ctgctgctgg acgatggtcg cgtccagtta aaagtactgg
     5701 aagtccaggg catgaaagtg ttcaccgaag tgaccgtcgg tggtcccctc tccaacaata
     5761 aaggtatcaa caaacttggc ggcggtttgt cggctgaagc gctgaccgaa aaagacaaag
     5821 cagacattaa gactgcggcg ttaattggcg tagattacct ggctgtctcc ttcccacgct
     5881 gcggcgaaga tctgaactat gcccgtcgtc tggcacgcga tgcaggatgt gatgcgaaaa
     5941 ttgttgccaa ggttgaacgt gcggaagccg tttgcagcca ggaggcaatg gatgacatca
     6001 tcctcgcctc tgacgtggta atggttgctc gcggcgacct cggtgtggaa attggcgatc
     6061 cggaactggt cggcattcag aaagcgttga tccgtcgtgc acgtcagcta aaccgcgcgg
     6121 taatcacggc gacccagatg atggaatcaa tgattactaa cccgatgccg acgcgtgcag
     6181 aagtcatgga cgtagcaaac gccgttctgg atggtactga cgctgtgatg ctgtcggcag
     6241 aaactgctgc tggtcaatac ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg
     6301 gggcggaaaa aatcccgagc atcaacgttt ctaaacaccg tctggacgtt cagttcgaca
     6361 atgtggaaga agccattgcc atgtcagcaa tgtacgcggc taaccacctg aaaggcgtta
     6421 cggcgatcat caccatgacc gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct
     6481 ctggtctgcc aattttcgcc atgtcacgcc atgaacgtac gctgaacctg actgctctct
     6541 atcgcggcgt tacgccggtg cactttgata gcgctaatga cggcgtagca gctgccagcg
     6601 aagcggttaa tctgctgcgt gataaaggtt acttgatgtc tggtgacctg gtgattgtca
     6661 cccagggcga cgtgatgagt accgtgggtt ctactaatac cacgcgtatt ttaacggtag
     6721 agtaagtacg ttgccggatg cggcgaaaac gcctcatccg gcctacagtt caatgatgat
     6781 tcaacagatt tcgaatcttc tgaagcaaac ttgaacttat catcagtcga aggcctctcc
     6841 tcgcgagagg cattttttat ttgatgggat aaagatcttt gcgcttatac ggttggattt
     6901 cgcccggttt gcgagttttc agcaatttta atatccaggt gtattgttct ggtcgcggac
     6961 caacaaaaat ctcgacttct tcattcatcc gccgcgcaat cgtatgatca tccgcctcta
     7021 acagatcatc catcggtggg cgcacctgaa tcgtcagacg atgcgtcttg ccatcataaa
     7081 tcggaaatag cggtacaacg cgcgcacggc acactttcat caaacgacca atcgcgggca
     7141 acgtcgcttt ataggtggca aagaaatcaa caaattcgct gtgttctggg ccatgatcct
     7201 gatcgggtaa ataatatccc cagtaaccct gacgtaccga ctggatgaat ggtttaatac
     7261 catcatttct cgcatgcaga cgaccaccaa agcgacgacg caccgtgttc cagacataat
     7321 caaaaaccgg gttgccctga ttatggaaca tcgctgccat tttctgccct tgcgaggcca
     7381 tcagcatggc aggaatatcg acggcccaac cgtgcggcac cagaaaaatc actttctcat
     7441 tattacgccg catctcttcg atgatctcca gcccttgcca gtcaacgcgc ggctgaattt
     7501 tctccggccc gcgtattgcc aactcagcca tcattaccat cgcttgcggc gcggtggcaa
     7561 acatctcatc tacaatcgct tcgcgttcag cttcactacg ttctggaaag cagagcgaca
     7621 gattgattaa cgcacgacgg cgtgagcttt ttcccagtcg tccggcaaaa cgtcccagcc
     7681 gtgccagaat gggatcacgg aactttggcg gcgttaaagc gatacccgcc atcgctgcta
     7741 cgcccagcca tgctccccag tagcgcgggt ggcgaaagga tttatcaaac tcaggaatgt
     7801 attcgctatt attttttttc gtttccatgc ttttccagtt tcggataagg caaaaannnn
     7861 nnnnnnnnnn nnnnnnngcg gcacaatctc tttggcctgt gccaggaatt cgcgacgatc
     7921 ggagccggtc agcccttcgg tacgcggcag ttttgccgtc agcgggttta cggcctgctg
     7981 gtttatccat acttcatagt gcagatgcgg cccggttgaa cgtccggtat taccggaaag
     8041 cgcgatacgg tcgccacgtt tcaccttctg tcccggtttc accaggatct tgcgcaagtg
     8101 catataacgc gtggtgtagc tgcgaccatg acgaatagcc acataataac ctgctgcgcc
     8161 actacgtttg gcaaccacca cttcaccgtc acccactgaa agcactggcg taccttgtgg
     8221 catggcaaaa tcaacacctc tgtgtggcgc aacgcgaccg gtcaccggat tagtacgacg
     8281 cgggttaaag ttagatgaga tacggaactg tttcgccgtc gggaatcgca agaatccttt
     8341 cgccagacca gtaccgttac gatcgtagaa tttgccatct tcagcgcgga ttgcgtaata
     8401 atctttacct tctgaacgca aacgtacgcc cagcagctgg ctttgctcac gtttaccatc
     8461 aagcatttct cgtgacatta acaccgcaaa ttcatcgcct tttttcagtt tgcggaaatc
     8521 catttgccac tgcatggctt taatcactgc gctcacttcg gcgctggtta aaccggcgtt
     8581 tctggcgctg gcaacaaagc ttcccccgac ggtacctttc agcagattgt tgacccactc
     8641 tccttgctgc atttcgctgg tcattttaaa accgttagcg gcagtacggt cataggttcg
     8701 ggtttcacga cgagacactt cccaggtgag gcgctgcagt tcgccgtccg cggttaatgt
     8761 ccaggagagt tgttgaccga ttttcaggtt acgcaattct ttgtcggcag cagccagttg
     8821 ggtgatatca cccatatcaa taccatactg attgagaatg ctgcttagcg tatcgccagt
     8881 ggaaacaaca tattcatgca cgcccgcttc accggcgatt ttgtcatcca gttcgtcctg
     8941 gggaatggct tcatcttctt gtgcagcttg atcaatcggc tcactggctt caggtaagag
     9001 cgaacgaatt tcgttctgtt ccagctcaat ggttttgaca attggcgtgg catcgcggtg
     9061 ataaacatag ggccgccaga cagcgacggc cagagtaaga acggtgagcg accccaacat
     9121 aacgcggtgt ggtcgcggta aattattaaa cgccagggcg acagagcggg ctatctgttg
     9181 cacgtaatca cttcctcatt aatctccttt caggcagctc gcata
//
LOCUS       DAWDRC010000081         9219 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3767_length_9219_cov_2.085661, whole genome shotgun sequence.
ACCESSION   DAWDRC010000081 DAWDRC010000000
VERSION     DAWDRC010000081.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9219)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3767_length_9219_cov_2.085661"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    277..286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1625..1634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3389..3398
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6047..6056
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6768..6840
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8469..8478
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2113 a         2254 c         2446 g         2283 t
ORIGIN      
        1 gcagtgagcg cagccttgac cgacctggaa gggcagcttg gcgttcaact gtttgatcgc
       61 gtggggaaaa gactggttgt taatgaacac gggcggctgc tctatccgcg agcgttggca
      121 ctgcttgaac aggcggttga aatcgaacaa ctgtttcgcg aagacaacgg cgcgattcgt
      181 atctatgcca gtagtaccat cggtaactac attctgcctg cagttatcgc ccgttatcgc
      241 catgattatc cgcagttgcc gattgaactt agcgttnnnn nnnnnngatg caagcggtgc
      301 tggatttccg cgttgatatt ggctttattg aaggaccgtg ccacagcact gaaatcattt
      361 ctgaaccgtg gctggaagac gagctggtgg ttttcgccgc gccgacttcg ccgttggctc
      421 gtggtccggt caccttagaa cagctggccg ctgcgccgtg gatcctgcgt gaacgcggtt
      481 ccggcacgcg ggagattgtc gattatctgt tgctgtcaca tttaccgaag tttgagatgg
      541 cgatggaatt aggtaactcc gaggcaatca aacatgcggt gcgtcatggg ttgggaatta
      601 gttgcctgtc gcgacgggtg attgaagatc aattgcaggc aggcacatta agtgaagttg
      661 cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg caaaaacacc
      721 tttccaacgc gctacggcgc tttctggact attgcgatcc cgcgaatgta ccgcgttaag
      781 gtgctgtaca agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc ctcagcatgc
      841 attcgccaga aaagagattg gctgctttac ttataatccc tgggcgatca tgaaggtgtc
      901 ttataaccgt gtatttctgc cggaaggatt gccaatcgtc tgctacaatc gcgcctcatt
      961 tttaagatgg atagcatttt tgtatgggtt ccgaaactaa aactacagaa gcgccgggct
     1021 tacgccgtga attgaaggcg cgtcacctga cgatgattgc cattggcggt tccatcggta
     1081 caggtctttt tgttgcctct ggcgcaacga tttctcaggc aggtccgggc ggggcattgc
     1141 tctcgtatat gctgattggc ctgatggttt acttcctgat gaccagtctc ggtgaactgg
     1201 ctgcatatat gccggtttcc ggttcgtttg ccacttacgg tcagaactat gttgaagaag
     1261 gctttggctt cgcgctgggc tggaactact ggtacaactg ggcggtgact attgccgttg
     1321 acctggttgc agcacagctg gtcatgagct ggtggttccc ggatacaccg ggctggatct
     1381 ggagtgcgtt gttcctcggc gttatcttcc tgttgaacta catctctgtt cgcggctttg
     1441 gtgaagcgga atactggttc tcactgatca aagtcacgac agttattgtc tttatcatcg
     1501 ttggcgtgct gatgattatc ggtatcttca aaggcgcgca gcctgcgggc tggagcaact
     1561 ggacaatcgg cgaagcgccg tttgctggtg gttttgcggc gatgatcggc gtagctatga
     1621 ttgtnnnnnn nnnnattgtc ggcttctctt tccagggaac cgagctgatc ggtattgctg
     1681 caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag gtattctggc
     1741 gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt ccgtataccg
     1801 atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc accctggtgt
     1861 tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt ctgacggcgg
     1921 tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtat accctggcgt
     1981 gtgacggtaa agcgccgcgc attttcgcta aactgtcgcg tggtggcgtg ccgcgtaatg
     2041 cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg tttggcaacc
     2101 agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc gcctggctgg
     2161 ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga cacgacatta
     2221 acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca ttcattctgt
     2281 gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc attgactggg
     2341 gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg ttcggctaca
     2401 agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg cagaacgata
     2461 agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct ttttcaattc
     2521 catttccctg acaaatcatg cggatataaa atttaacatt tggattgata attgttatcg
     2581 tttgcattat cgttacgccg caatcaaaaa aggctgacaa atcagaggct gttccggctt
     2641 tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac gcaaggggac
     2701 gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg agatatggaa
     2761 tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt tcttgtgcat
     2821 ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca tcttccgttg
     2881 aacaaaacct taaagatgct cccgccagta tcagcgtcat tacccaggaa gacctgcagc
     2941 gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta caactgacga
     3001 acgaagggga taaccgtaag ggcgtaagta ttcgtggtct ggacagcagc tacaccctga
     3061 ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac aatgatttcg
     3121 atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt ggcccgatgt
     3181 cgtcgctgta cggttccgat gcgctcggcg gtgtagtgaa tatcatcacc aaaaaaatcg
     3241 gtcagaaatg gtcgggcacc gttaccgtcg ataccaccgt tcaggaacat cgcgatcgcg
     3301 gtgataccta taacggtcag ttctttacca gcggaccatt aattgacggc gtgctgggac
     3361 tgaaagctta cggcagcctg gcaaaacgnn nnnnnnnngc gtattgaagg attctccagc
     3421 cgcgacggca atgtcgaatt tgcctggaca ccgaatcaaa atcacgattt tactgccgga
     3481 tacggtttcg accgtcagga tcgtgattcc gactcgctgg acaaaaaccg cctggaacgc
     3541 cagaactact ccgtcagcca taatgggcgt tgggattacg gcaccagcga actgaaatac
     3601 tacggtgaga aagtcgagaa caaaaaccct ggcaacagca gcccgataac ttccgaaagc
     3661 aatacggtcg acggcaaata cacgttgccg ctgacggtga ttaatcagtt tctcacggtt
     3721 ggcggtgaat ggcgtcacga caaacttagc gatgcggtga acctgaccgg gggaaccagc
     3781 tccaaaacgt ctgccagcca gtacgcgctg tttgtggaag atgaatggcg gatcttcgag
     3841 ccgctggcgc tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg tgaacactgg
     3901 agtccgcgtg cctacctggt ttataacgcc accgacaccg taacggtgaa agggggctgg
     3961 gcgacggcat ttaaagcccc ttctctgttg caacttagcc ctgactggac gagcaattcc
     4021 tgccgtggcg catgtaagat tgtgggtagc ccggatctga aaccagaaac cagcgaaagt
     4081 tgggagctgg ggctttacta catgggtgaa gaaggctggc tggaaggggt tgaatccagc
     4141 gttaccgttt tccgtaacga tgtgaaagat cgtatcagca ttagccgtac gtctgacgtc
     4201 aatgctgcac cgggctacca aaactttgtc ggttttgaga cgggcgctaa cggacggcgc
     4261 ataccggtat ttagctacta caacgttaac aaagctcgta ttcagggcgt ggaaaccgaa
     4321 ctgaaaattc cgttcaacga tgaatggaaa ctgtcgatca actacaccta caacgatggt
     4381 cgtgatatca gcaacggcga aaacaaaccg ctatccgatc tgccgttcca tactgctaac
     4441 ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt ttctggtcac
     4501 tataccgggc agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg ttataccatc
     4561 tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc aggcgtgctg
     4621 aaccttggcg acaaggatct cagtcgtgac gactacagct ataacgaaga cggacgtcgt
     4681 tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac cgggcggact
     4741 ttcacttcgg taaatactgc gcatggaagc gcaggtgatc ctctataaaa gaggcgatga
     4801 agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac gctttctggc
     4861 gcgcggcttc tgccagtact gcaggttgca gttggtcagc aagaaactga tcattatcac
     4921 cctgatcaat aagcgtcggg attgcatcct gcgcgttact ggcatacatc aacgcgcaac
     4981 tgtcccattc cagccatgca tttttgtcct cacctaaata agtgctaaac gctttgattc
     5041 cccacgggac gctgcacgga ttcacaattg gcgcaaaggc ggaaacgctg gtgtatttac
     5101 ccggattttt cagcgccata atcagcgcac cgtgaccacc catggaatga ccgctaatag
     5161 cgcagcggtc gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat
     5221 aatcatacat ccggtaatgc gtcgcccacg gtggttgcgt ggcgttaaga taaaaccccg
     5281 cgccctggcc taaatcgtag ccatcgtcgt tggcaacctg ttcgccgcgc gggctggtgt
     5341 ctggcatcac cagtacaatc cccagttccg ccgccacgcg ctgggcaccc gccttggtgg
     5401 tgaagttctc gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt
     5461 gatcacgagg tggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt
     5521 cgtgtcgcca gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc
     5581 gtggctcctt gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg
     5641 agtaactttc acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc
     5701 ggcaattaca ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata
     5761 atcaggctgt aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg
     5821 caggagaaat cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta
     5881 gttgcgcgag gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg
     5941 cgcaaaagcc ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg
     6001 gctgatgaca ggttgatgga acagccacat cgcaacggcc aaaagtnnnn nnnnnntgta
     6061 tgtcgatgaa attttctccg gtctggatta cgccaatttc ccgaaaatca ccctcattga
     6121 aaacaaaatg aaggtcgatg aaatggtcac cgtgcgcgat atcactctga ccagcacctg
     6181 tgaacaccat tttgttacca tcgatggcaa agcgacggtg gcctatatcc cgaaagattc
     6241 ggtcatcggt ctgtcaaaaa ttaaccgtat tgtgcagttc tttgcccagc gtccgcaggt
     6301 acaagaacgt ctgacgcagc aaattctcat tgcgctacaa acgctgctgg gcaccaataa
     6361 cgtggctgtc tctatcgacg cggtgcatta ctgcgtgaag gcgcgtggca tccgcgatgc
     6421 aaccagtgcc acgacaacga cctctcttgg tggattgttc aaatccagtc agaatacgcg
     6481 ccacgagttt ctgcgcgctg tgcgtcatca caactgataa aaaggcagga accgtggagc
     6541 gcaacgtcac gctcgatttt gttcgcggcg tcgccattct ggggatcctg ctattaaaca
     6601 tcagcgcctt tgggttacca aaggcggctt atctcaatcc cgcctggtac ggcgctatta
     6661 cgccacagga tgcatggacc tgggcatttc tcgatctcat cggccaggtg aaattcctca
     6721 cgctttttgc gctgttgttt ggtgcgggcc tgcaaatgtt gctgcccnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 agtatggctg gcaactggca gggatgatgc tcattggtgc cgcattgatg cgcagtggct
     6901 ggctgaaagg gcagttcagc tttcgtcact atcgtcgtac tggttttgtg ctggtggcga
     6961 ttggagtgat cattaacctt cctgccatcg ccctgcaatg gcgactggac tgggcgtatc
     7021 gttggtgcgc cttcttactt cagatgccgc gggaactgag tgcgccgttt caggcgattg
     7081 gctatgcgtc gctgttttat ggcttctggc cgcaattaag ccgctttaag ctggtgctgg
     7141 caatcgcctg tgtcggacgg atggcactga ccaactatct attgcaaacg ctgatttgta
     7201 ctacgctttt ttaccacctc ggtttgttta tgcagtttga ccgtctggag ctgctggcgt
     7261 ttgttattcc ggtatggttg gcgaatatct tcttctctgt tatctggctg cgttacttcc
     7321 gccaggggcc ggtggaatgg ctctggcgtc agttaacttt gcgtgctgcc ggaccgacaa
     7381 tatctaaaac atcaagataa cgatctggat cacattcgtt aacaaaacgg ctgtaaccgt
     7441 ttccattgct gtgactcgat tcacgaagtc ctgtattcag tgctgacaaa atagccgcca
     7501 gcaagcagtc atttactgca atctcataac aggtagtgaa tatgatcacc attcgtgatg
     7561 tagcgcgtca ggctggcgtc tctgtggcaa cggtttcccg ggtgctcaat aacagcacgc
     7621 tggtcagtgc cgatacgcgt gaagcagtaa tgaaagccgt gagtgagctg gattatcggc
     7681 caaacgccaa tgcccaggcg ctggcaactc aggttagcga taccattggc gtggtggtga
     7741 tggacgtttc tgatgcgttt ttcggcgcgc tggtaaaagc ggtggatctg gtcgcccagc
     7801 agcatcagaa atacgtgcta atcggcaata gctatcatga agcggaaaaa gagcgtcacg
     7861 ccattgaggt gttaattcgc cagcgttgta attcgttgat tgttcacgca aaagcattga
     7921 gtgacgatga actggcacaa tttatggata acattcccgg tatggtgtta atcaaccgcg
     7981 ttgtgccggg gtacgcccat cgttgcgttt gcctggataa tctcagcggt gcccgaatgg
     8041 cgacgcgcat gttgctgaat aacggtcatc aacgtattgg ttatctttct tccagccacg
     8101 gcattgaaga tgacgccatg cgtaaagcag gctggatgag tgcgttgaaa gagcaggata
     8161 ttattccgcc ggaaagctgg attggcactg gtacgccgga catgccgggc ggtgaggcgg
     8221 cgatggttga actgctgggg cgcaatctac aactaaccgc tgtatttgct tataacgaca
     8281 atatggccgc tggcgcactg acagcattaa aagataatgg cattgcgatt ccgttacatc
     8341 tctcaatcat tggtttcgat gatattccca tcgcccgtta caccgacccg caattaacga
     8401 ctgtgcgtta tcccattgct tcaatggcta aattagctac cgaactggcc ttgcaggggg
     8461 cagcagacnn nnnnnnnntt ctgtcgcaac acgccagaat gcggcggcga tcactaactc
     8521 aacaaatcag gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat
     8581 gtgatggcta tgattgcacc gttttaacgt tgtaacccgt atgtaacagt gaataatcac
     8641 tttcaccgag gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg
     8701 catggaagaa cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta
     8761 ctgccccgcc gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat
     8821 gaataagaag gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc
     8881 acacgctgct gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc
     8941 tgtagtgcgc aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat
     9001 gaatgattct cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa
     9061 aggggtgaag gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga
     9121 gaaagcgcgt gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc
     9181 gctggatagc tacgacaaag cctactacgt tggcactga
//
LOCUS       DAWDRC010000082         9162 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3791_length_9162_cov_2.212035, whole genome shotgun sequence.
ACCESSION   DAWDRC010000082 DAWDRC010000000
VERSION     DAWDRC010000082.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3791_length_9162_cov_2.212035"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    463..472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4316..4325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6500..6509
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    8745..8754
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2220 a         2449 c         2331 g         2122 t
ORIGIN      
        1 cggctgggca accagtggtg taccaccgtt actggcgcta atcgctacat tgtaagaacc
       61 gttcggcgca gtgctgccgt cagtcaacgt accgtcccag gtgaaactgt ggactccggc
      121 ggtcagttca ccaatatcaa tggtgcgcac aaccgcgcca tttttatcgg tgatggtggc
      181 cgtcactttg tctgccgcct gttgcagctc aacaccaaac ggcgtagtcg tggtcacagc
      241 cccttcttca ctgccggttc ccgcaagaac agtggtgccg gggatcatca cgccgtgacc
      301 gatcaggtta ctggcctgta acgactggct gttatcaatc tgtccggaaa tagatccgag
      361 cgtggtattg agtttttcaa tcccgctgac cgtgctgatt tgagccaatt gcgacgtcag
      421 ctcgttgttt tccattggat tggtcgggtc ctggtttttc agnnnnnnnn nntgcgccac
      481 cagcaaagtc agaaaactgc tttgtaaatc tgaggcgttg ctgcccgtga gcgaactact
      541 actggtggtg ctgacgccgg tatttgtcgg atcggtggtg gttaccgcaa tggacatagt
      601 tttctccttt attgaccgag cgtaagggtt ttcagcatca tgcttttcac cgtgttgagc
      661 acttcaacat tggcctgata gctgcgtgac gccgacatgg tgttaaccat ctctccgacg
      721 acatcaacgt ttggcatttt cacgtagccc tttgcatccg ccagcggatt acccggttca
      781 taaaccagtt tgtccggggc ctgactttct ataacatcgg caacctttac gccgcctgtc
      841 gcagcacctg gtgcagcgtt aacctggaat accacctgtt ttgcccgata tggctgtcca
      901 tcgggaccgg tcacgctatc agcattcgcc agattactgg ccgccacgtt caggcgctgg
      961 gactgggcag ttaacgccga cccggcgata tcaaaaatat tcagcagtgc catccgttaa
     1021 tttccgctct gtaaaacgtt catcatgcct ttgatctgcc cgctcaacac gctaagactc
     1081 atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt cgcgatccat atcgacggta
     1141 ttaccgtcaa gcgaaggctg gtccggaata cggtattgca gttctgcggt aggaggcgtc
     1201 agcgcctgcg ccggaatgtg ttgcgttgag gtcatcgtca gtgcaaccac actggttgca
     1261 tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc gcgcgcctga
     1321 taaccagggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg ctgggcgcgc
     1381 agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag catatctcct
     1441 ccgcaagtat caaaattcgg ccatcagctt aaatgccttt acgcgcgcgt tatcggcgga
     1501 ataaacgcaa aatgggtctc tatttatgcc gttgatggtc attgcggaca ggtacaattc
     1561 acgttgtaga aatggctggg gggcgaaaat gctggcatta aaacgtagcg tggcgatcat
     1621 cgcgatactg ttcagtccgt taagtgcggc gagcaatctc acatcgcaat tgcacacctt
     1681 ttttagcgcc cagctcgcgg gggtaagtga tgaggttcgt gtttctattc gtacagcgcc
     1741 caatctacta ccgccgtgcg agcagccatt gctttcgatg agcaataatt cccgcctgtg
     1801 gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa cgatatttac aggttaatgt
     1861 gcaggccaca ggaaattatg tggttgccgc aatgcccatt gtgcggggag gaaagctgga
     1921 agctggcaat gtcaaactga aacgcggacg gctggacacc ttgccaccgc gtacggtgct
     1981 ggatatcaat caacttgttg atgccgttag cctgcgcgat ctatcacccg atcaacctat
     2041 ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg ggacaacgcg tcaacgtgat
     2101 cgccagtggt gatgggttta gcgccaacgc agaaggtcag gcgctgaaca atgcagccgt
     2161 cgcacagaat gcgcgggtgc gcatggtatc gggacaggtg gtcagcggcg ttgttgatgc
     2221 agatgggaat attcttataa acctgtaagc tgttaaagat tacccgtccc ttgccgataa
     2281 ataagcaaca catgataaaa gcgccctcaa tgaggaataa accatgagta ttgatcgcac
     2341 ttcgcctctg aagcccgtaa gcaccgttca accgcgcgaa accattgacg cgccggtaac
     2401 gaacacccgg gcggcaaaaa caaccgcctc caccagcacc agtgtgacgt taagcgacgc
     2461 gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt
     2521 aaaactggcg attcgtaacg gtgaactaaa aatggacacc ggcaaaattg ccgatgcgct
     2581 gatcaacgaa gcgcagcaag acttgcagag taactgatcg tatgacgcgt cttgcagaga
     2641 tcctcgacca gatgtccgct gtgcttaacg atctcaaaac ggtaatggat caagagcagc
     2701 aacatctctc tatggggcag atcaacggca gccagttgca atggattaca gaacaaaaaa
     2761 gctcactgct ggcgacgctg gattacctcg aacagttacg caggaaagaa cccaatgcag
     2821 caaatagcgt tgatattagt caacgctggc aggaaattac tgtgaaaacg cagcaactac
     2881 gccaaatgaa tcaacataac ggctggttac tggaaggaca gattgagcgc aatcaacagg
     2941 cgctggaaat gttgaaaccg catcaggaac cgacgctata tggagcgaac ggtcagacct
     3001 caacaaccca tcgcggcggt aaaaagattt cgatctgaag attatctccg gcctgcactg
     3061 caggccggaa tgcattgtta caccgtccgg cgggcaaact ctttaacttt gaagcccagt
     3121 accgccagtg cagcaaagta cgcagcaatc cccgccagca cgaccgccat taaacgcagt
     3181 aagcgccagg gcatggtacc caatgaccac tccggcatga tatgtaacat acctaaaagc
     3241 acgcccgaca tcaccagtac cgccaccacc agacgcaaca gaaacgccat ccagccaggt
     3301 tgcggggtaa agattttctg cttacgcaac tgccagtaaa gcagcgaagc attcagacac
     3361 gccgccagac caatagaaag tgacagcccg gcatgtttca acggaccgat aaacgccagg
     3421 ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt tcactggcgt tttaatgtcc
     3481 tggcgggaat aaaagccagg agccaacact ttcactacaa tcaggccaat caaacccacc
     3541 gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa aggcggtaaa tttaccgtac
     3601 tggaacagcg aaacggttaa cggacctgaa agaatgccca acgcaaccgc actcggcagc
     3661 gccaacagga aacaaagacg cagcccccag tccatcaaac ggttgtattc atcatgattg
     3721 ccactggcaa aacttttcga cagcgacggc agcaaaatgg tgccaagcgc cacgcccagt
     3781 acaccggacg gaaactccat taagcggtcg gcgtaataca tccatgacac cgaaccggaa
     3841 gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga tctggctcac agagacgcca
     3901 aggattgccg gtcccatctg tttcaccaca cgcattgctc cggcatcgtg gaagttaatg
     3961 cgtggcaaaa ccagcatgcc gatcttcttc agatgaggta gctgatacac cagctgcagg
     4021 atgccgccga ccgtaacagc ccaggccagc gccagcaccg gtggattaaa gtacggcgcg
     4081 gcaaacagcg caaagccaat catgctgata ttgagcaatg tcggggcaaa cgccggaatt
     4141 gagaagcggt tccaggtatt cagaatcgct cccaccagcg acgccaggga gatcagcaag
     4201 atatagggaa aggttatctt tagtagctgg ctggtcaggg caaatttgtc ggcagtgtca
     4261 gcgaagccag gcgcggtcac catgatcacc cacggtgcag cgagcatgcc agcgannnnn
     4321 nnnnntcaca accgccagcg caagtgtcag caggccagaa acataagaga caaagacccg
     4381 cgtggcgtct tcaccctgct tgcttttata ttctgccaga atcggtacaa atgcctggga
     4441 aaatgcccct tcggcaaaga tacggcgtaa caagttagga agtttaaaag cgacgaaaaa
     4501 ggcgtcggtt gccatccctg cgccaaagat tctggcgaca attgcgtctc gtgcgaagcc
     4561 aagcacacgc gaaaacatgg tcatcgagct gacggcggcc agcgatttta ataaattcat
     4621 cggtgttcta atccagaccc acgggcaaaa cgcctgcaag gcaggcgctg aaagaggcga
     4681 atagtctacc tggattatgg tgaattgcta ccgccagatg ttacagggtt tattcactca
     4741 tcgcatcgcg ccagatcttg tcaacgatac gttgcgccag cacggcctgt tcgccggcgg
     4801 tttgcggaac tgtctgattt tgcacgcatt caatgaagtg ccgcgcacag ccgacaaatc
     4861 cacgttgctc gagcgtactc tgccaaccag gaatcggttt atgcactacg ccctgcccgc
     4921 gctcctcacg ccattcgcgc atatccgtaa tgtcgatgag cgcaccatca gtcacggcct
     4981 gcacggtttc acgctgactt ccggcacggc gatgcataca ggtggtgatc tgcaaaggac
     5041 cagccgaaaa atggtgctcg gcaaacagca tttcgccgga ctcattggtc agcaacgtac
     5101 cgctttcgag agttgcattg ccgccagaca accacagcgc ggtatccacc acatgcagat
     5161 aatcatccag caacgtgaaa taaagatcgt gtggcccgac gctattgcta cgatgtttat
     5221 ccattcttag cgaggacgcg gtggcgagtt gcgtttttaa ctcaccgtag agtggtgcga
     5281 aacgacggtt aaaaccgacc atcaacgtca gttttttccg cgccgccagt tccaccagcc
     5341 gttcagcatc gcgcagattt tctgccagcg gtttatcgac acagacatgt acccccgcat
     5401 tgagtaacgt actgaccaca tcaaagtggc tggcggtgct ggaatgcaca aaaaccgcat
     5461 cgcaactggc ggcaaggctg gataacgaat cagcataagg aatgcgccag ctgtcacaaa
     5521 ttggcaaggc tttggcgcgc gtaggcgacc acgcaccttg taacgtccag tcagacgctg
     5581 ccgccagcac cggtaaccac gctttttgcg cgatgccgcc taaccccact acgccgatac
     5641 gtaatttttt cactttaatc tcccagatgg gccagcaacg aatcaagacg ctgtttcagt
     5701 tctgccactt cgatttccag ggcttcgaca cgggcctgta aatcaccgtc aaccgcgttc
     5761 aacatatccg ttaccgccgg cggattctca acctcaccac tgaaaagatg catgtagcgg
     5821 ctttcgcgtt tacccggttc gcgggccaga cgcaccacaa aaggaccatc ttcgcgactc
     5881 gccagttgtt ccagcgtcga ctccacttcc gccatatcgc tgaattcata cattcgcgcg
     5941 gcgcggctgc gcagttcccc cggcgtctgg gcaccacgca ataacaacgt ggtgattaac
     6001 gccacttctg cggcgctcag tttcagatcg ccaaattctg aattacaaaa acgttgctca
     6061 tatttggtga cccgattacc aaaaccgctc accgtgcgta gataatgacg tttgaccaga
     6121 ttatccagct gttcctgcac ttcagattca ctcagattca taaccggttc acggttcgtt
     6181 ttctgattac aggccgtgac tacaccattt acggagagcg ggtattgctc cggcgtggtc
     6241 acctgttttt ccagcaagca accaatcacg cgagcttcca tggcagtaag ttgatatttc
     6301 atgagattct ccttagcggc cgggcgtcca gtctggggta gttaatgccg tcagtacgtg
     6361 atcgcgccat tgtccatcaa tcaacagata gtctttcgca tagccttctt tttcaaaacc
     6421 cagtcgcgcc agtaaatcac cgctgcgttt attgtgcggc atataattag ccataatgcg
     6481 atgaatatgt tgggtgcgcn nnnnnnnnna atggctgcgc tcagagcttc aaacatgagt
     6541 cctttgccct gccatttttg cccaatcgaa taaccgagat agcaggcatg aaaagagccg
     6601 cgaacaacat tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg gtcgaataag
     6661 ccaaagtaga aagctgaacc ttgtttatga aattcgttaa tcatccctag cctggcctgc
     6721 cagcctgatg gataacagtg gctttcgtcg cgcactggct cccagggctt gaggaaatgg
     6781 cgattctctg cgtaataatc cgccagacgc caggcatcac gatcatgcac cagacgcacg
     6841 accagtcggt ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc aaacattcta
     6901 tacctactcc ttcccgtaac gtctttaata cgctgtaagt attactatac ctgcgcaacc
     6961 agaccctgtg aaaagtccag aatgccattt tctgcattat tcacatattt gataaaaact
     7021 ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt
     7081 agcggcaatg cagaatagaa agtgctcatc ttttatttcc cgggagggga aatgtcccgc
     7141 gtgtcgcagg cgaggaacct gggtaaatat ttcctgctca tcgataatat gctggtcgtg
     7201 ctggggttct ttgttgtctt cccgctgatc tctatccgct tcgttgatca aatgggctgg
     7261 gccgccgtca tggtcggtat tgccctcggt ctacgccagt ttattcagca aggtctgggt
     7321 attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt caccggtatg
     7381 ctgatgcgtg ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg gctactgtgg
     7441 ttttcatgcc tgctttcggg actcggcggc acgttgtttg atcctccgcg ttcggcgctg
     7501 gtggtgaaat taatccgtcc acagcagcgt ggtcgttttt tctcgctgtt gatgatgcag
     7561 gacagtgccg gtgcggtcat tggcgcattg ttggggagct ggctgttgca atacgacttt
     7621 cgcctggtct gcgccacagg ggcagttttg tttgtgctgt gtgcagcgtt caatgcgtgg
     7681 ttgttaccgg catggaaact ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc
     7741 gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc tggcgggtta ctacatgctg
     7801 gctgtacaag tgatgctgat gctgccaatt atggtcaacg acgtggcggg cgcgccctct
     7861 gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct ctaccctatc
     7921 gcccgctgga gtgaaaagca ttttcgtctg gaacaccggt taatggctgg gctgttgata
     7981 atgtcattaa gcatgatgcc agtaggcatg gtcagcggcc tgcaacaact tttcaccctg
     8041 atttgtctgt tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct
     8101 tcgctggcag acgcaagagc tcgcggcagc tatatggggt ttagccgtct gggtctggcg
     8161 attggcggcg ctattggtta tatcggtggc ggctggctgt ttgacctggg caaatcggcg
     8221 caccagccag agcttccgtg gatgatgctg ggcattattg gcatcttcac ttgcgctggg
     8281 ttggcagttt agccagaaac gcgccgcgcg tcgtttgctt gaacgcgacg cctgatttga
     8341 tactcccccc tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt
     8401 gaagctatac atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct
     8461 gaaaaatatc cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg
     8521 gatggtcggt caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga
     8581 aagcatggac atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa
     8641 acgttcccct gccattgaag agtggctgcg caaggtcaat ggctacgcca acaaactgct
     8701 gttgccgcgt tttgccaaat cggcatttga tgagttttct actcnnnnnn nnnntttcta
     8761 ctcctgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg
     8821 acctgctggc ccactctgac ggtctgatta agaatatcag cgatgattta cgtgcgctgg
     8881 acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc
     8941 tatttccgct cctgcgtaat ctgacgctag tagccggaat caactggcca agccgcgttg
     9001 ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga
     9061 tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgctgttt tcagtttttt
     9121 ctgctggcgc gtttatcgct ggtgacacat gctgcgttgt gt
//
LOCUS       DAWDRC010000083         9079 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3822_length_9079_cov_2.412456, whole genome shotgun sequence.
ACCESSION   DAWDRC010000083 DAWDRC010000000
VERSION     DAWDRC010000083.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9079)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3822_length_9079_cov_2.412456"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1804..1813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2234..2243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2334 a         2470 c         2190 g         2065 t
ORIGIN      
        1 cgccatagcg aggtgcaggc attcaggaaa ctgcgggtca tgctgatgag attccaggac
       61 tgccccggac gcgccgccgc cagtttggga atgtagatat tggcaacgac cgcgccaccg
      121 taggccagtg cgcatgcggc cagggcaacc agaatatgcc agtcagccag aacaccacca
      181 gctacggaac cgagcaaaat cgccgctatg gtagacgctt ccattaaacc gttagctttt
      241 actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg tgaataggct
      301 gcagcaccaa cacccaccaa cgtatagccg agaaacggat tgataccaaa gcagatactg
      361 gctgcgccca gtagcttcag gccgttggca aacatcatca cccggccttt ggcgaagcta
      421 tccgccacct gcccgacaaa cggcgcaaaa agaatgtaag cacctacaaa caccatttgc
      481 aggatgggct ggctccactc cggatagaac tgcgctttca gtaacgccag agtggcaaac
      541 agcagcgcat tatcgccaaa cgcagagaga aactgcgcca cgataaccgc tttcatcccc
      601 ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc
      661 tacccagctt ttcaacgtga caaagtcagg tttgccgctg ccaagtaatg gcatctgttt
      721 cagatagcga atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc gggcatactg
      781 ttgtaactta tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt
      841 gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac caagtgccag
      901 ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg
      961 gccctgaatc tgcacaaagc cctgctcatc aaaacgcacg atatcgccag tgtcatacca
     1021 gccgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta caccaggctt
     1081 ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc gtccgccctc
     1141 ttcgatacca ggaactgaca acaggcgcgc gtccatacct ggtagaatac gccctaccgt
     1201 accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcact cggtcacacc
     1261 gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttgg tactttcttg
     1321 tagtttttct gcgcctgcca ccacatagcg tagacgatag aagtcatacg ggttggcgaa
     1381 acgcgcgtag tgaccgagga aagtcgaggt accgaacaac acggtgcaac tgcggtcata
     1441 caccaactcc ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca cttctgcacc
     1501 tgtgagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca gcggtaacgc
     1561 cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct cgacgttcgc
     1621 cagaatgctt ttatggctat ggacgacgcc tttcggatgg ccttcagaac cggaggtaaa
     1681 gaggatcagc gcctcttctt ccggctgctg ttttaactgt gccagacgcg gcatcagcaa
     1741 atgagcgaaa atccacactt tgtcggcagt ggtgacatct gcttttaaat cttccagata
     1801 gacnnnnnnn nnngcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat
     1861 cgagaaactg acgggaagtg aaaatggttt tgatttcagc cgccgtaata gcactggtca
     1921 acccttttac cccggcggtg tagttcatca ttgcgggaat gcggcgacgg gcgatggccc
     1981 caaaaatcac tgcagcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt
     2041 caacactgta tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat
     2101 aggagtccgg ggtaaagtta acgtcttcga cacatttctt cccggctccg aagcggtaca
     2161 ttgcgctcag taaagattcg tacagcgttt cacgcgggcg caccgccata cgcgcctcca
     2221 tcattatttg atgnnnnnnn nnnatgcagc atttcgccag cgattttgcg acggtcacgg
     2281 gcacgcggcg catccggcat ctccacctgc gttggtggca aaatatgcag agtaatttgc
     2341 gggaacaagc gacgtttaac cagacctttc aggcggctga agtgcgtaag ttccgcccct
     2401 tcaatacgca caggaataac cgttgcacca gacttcgccg cgacaaatcc cgcgccatcg
     2461 tagattttca tcagcgagcc agtggtggtg atgcgtcctt cagggaaaat caccactggt
     2521 cgtccctgtt ccaccagacg taccagatgt ttaatcgcca taggttgcgt cgggtcgaga
     2581 ggaacaaagt cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg
     2641 taaacggcaa ataccggacg tacgggtaaa aacaatgcca gcagtatgcc atcaataaaa
     2701 gagacgtgat taggcgtaat tagaacgcgc tcgcccttca gtgccttggt gtcacccgta
     2761 acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact
     2821 ccctttgctc tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga
     2881 gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac
     2941 tgggtacgaa gagcgtaccg aataatctca ccaatcaata cctctgggat cttgattgtg
     3001 gtctgcacaa cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga
     3061 ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat
     3121 ctgcgcaggc tggtgtaatt catgtgctca acccgaagtt gacttcacct atcaatacct
     3181 ctgggatcac cactttagca acctgaagcc aaacgccacc agcagtcaat cgcaacagca
     3241 tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg
     3301 gctcacattc ccacgatgaa aacacgccac cccttgaacc aacgggcgtt ttccgtaaca
     3361 ctgaaagaat gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag
     3421 cccgactggc aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag
     3481 ccagcgaagc ttcccgactg gctgtgcata gtgcaatgga gtctcttagc tatcacccga
     3541 acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg gtcgttggtg
     3601 atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca
     3661 ctggtaattt tttattgatt ggcaacggtt accacaacga acaaaaagag cgtcaggcca
     3721 ttgagcaact gatccgccat cgctgtgctg cgctggttgt ccatgccaaa atgatcccgg
     3781 atgccgattt agcctcatta atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc
     3841 tgcctgactt tgaaaaccgt tgtattgctc tggacgatcg ttacggtgcc tggctggcaa
     3901 cgcgtcattt aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta
     3961 tttctgacgc cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattc
     4021 cggccaatga ccggctggtg acatttggcg aaccagacga aagcggcggc gaacaggcaa
     4081 tgaccgagct tttgggacga ggcagaaatt tcactgcggt agcctgttat aacgattcaa
     4141 tggcggcggg cgcgatgggc gtgctcaatg ataatggtat tgatgtaccg ggtgagattt
     4201 cgttaattgg ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg
     4261 tgcgttaccc aatcgtgacg atggccacgc aggtacgtcg ccattcagtg tcaactccgt
     4321 cgctggaggc aagtcatcat gcaaccagcg actaaccgca gttaaagcaa ttccagcgcc
     4381 agtaattctt caatggtctg gcgacggcga atcaaccgcg cctgaccatt atcaaacaga
     4441 acttctggta acagcggacg gctgttgtag ttggatgaca ttgatgcgcc atatgcccct
     4501 gtatcatgca gtaccagata atcacccgcc ttcacttccg gcaaggcgcg ggtttcgaca
     4561 tttccccctt cctgctgggt aaagacatcg cccgattcac ataacggccc ggcgacgacg
     4621 gtttccaccg ttggcgcgtg ttccagagaa cgaccctcag ctgccagggc actgatatgg
     4681 tggtaactac cgtacattgc cgggcgcatc agatcgttga acccggcatc aaccagcaca
     4741 aagtggcggc tacccatttg tttgacgctc cgcacttgag taattaatac gccagcctgc
     4801 gctaccagaa agcgacccgg ttcaatttcc agtttcacag gatggcccaa atggcgggcg
     4861 atttgctcac gcgcggcatt ccacagacca taataatgtt cggtatcaac cgcctcttca
     4921 ccctgttgat aaggaataga aagcccaccg cccgcagaaa tagcctgtaa atcctgaccg
     4981 aactcgagga cctgacgcac catagcacca catacctgtt ccagatgggc ataatcgacg
     5041 ccagaaccaa tgtgcatgtg aatgccgacc agctgtagat gatgacgttg tatcacgtcc
     5101 agtgcggcgg gcagatcggt gtaccagata ccgtgcttgc tgttttcgcc accggtattg
     5161 gttttttggc tatgcccgtg accaaacccc ggattaacac gcagccatac ccgatgccct
     5221 ggagaaacct gacccagttg gtcgagcata tcaacagaac ccgcattcac cggaatttgc
     5281 aattcactga cgcgttcaag cgtcgcctga tcgataacat ctgccgtaaa aacaatatca
     5341 tcggggtgcg tttgcggatt gtaacccgcc gccagtgcac gctctatttc gcctaacgag
     5401 acggaatcca cttttacgcc ctgctcacgc attaagcgca aaatatgaat attggaacag
     5461 gctttctgtg caaagcgcac cacatcaaac tgtttcagcg ctgcaatctg ccgacgaata
     5521 atttgcgcat cgtagaccca caccgggcag ccaaattctg cgggcaaacg cagcagattt
     5581 tcggcggtga gatcggtatc agtgctgaac agtgaatgtg gcataacaaa ctccagataa
     5641 gtgctttttt atgattacgc cacatcataa aaagaataaa aaatatcgat ttatgtcgag
     5701 tctatgcaaa aatgatatgg attaccggat tgcgagagag cgctaatggc cgccgttaac
     5761 ttacgtcata ttgaaatttt tcatgcggta atgaccgccg gaagcctgac tgaggcggca
     5821 cacctgctac acacctcaca gccaaccgtc agccgcgaac tggcgcgctt tgagaaggtg
     5881 atcgggctga aattgtttga gcgcatacgt ggacgattac atcctaccgt gcaaggactg
     5941 cgtctgtttg aagaagtgca acgatcctgg tacggactgg atcgcattgt cagtgccgca
     6001 gaaagtctgc gcgagtttcg ccagggagaa ctgtctattg cctgcctgcc ggtcttttcg
     6061 caatcttttt taccgcagct cctgcaaccc tttctggcac gttatcccga tgtcagctta
     6121 aatatcgtgc cccaggaatc accgctactt gaagagtggc tctcggccca gcgtcatgat
     6181 ttaggactca ctgaaacgct ccatacgcct gcgggaacag aacgtaccga attactctct
     6241 ttagatgaag tgtgtgtgtt acctccgggc catccgctgg cggtaaaaaa ggtattaacg
     6301 ccggatgatt ttcacggtga gaactacatc agcctttccc gtactgacag ctatcgccag
     6361 ttgctggatc aattgtttac tgagaatcag gttaaacgac gcatgatcgt agaaacccac
     6421 agcgccgcgt cagtctgcgc aatggtacgg gcgggggtag gcgtttcggt ggttaacccg
     6481 ctcaccgcac tggattatgc ggcaagcggt ttagtggtgc ggcggttcag cattgcggtt
     6541 ccattcaccg tcagcctgat ccgccccctg caccgcccgt catcagcgct ggtgcaggcg
     6601 tttagtgagc atttacaagc gggattaccg aaactggtca cttctcttga cgctattttg
     6661 tcgtcagcta cgacagcata aaagcgacag catcctcggc atggatcgcc gcggtatcaa
     6721 acacaggcag aacactgcgc tcttctggca ctagtaaacc aatttctgtg cagccaaaaa
     6781 tgacgccctg tgcgccctgt tctgcaaggc gagcaatcac ttgcgcataa taagcgcgtg
     6841 acgcttcggt aaattgcccc agacacagtt cttcaaaaat aatctgatta atttttgctc
     6901 gttcatccgc ttcaggaata agacagttga tggaaaattg ttccgtcagc cgcccgcgat
     6961 aaaaatcctg ttccatggtg taacgtgtac ccagcagcgc cacacgagtc attcctgccc
     7021 cggtaattgc acgtccagtg gcatccgcta tgtgtaagaa aggcagagag caacgagact
     7081 caatggcatc cgccactttg tgcatcgtat tggtgcataa cacaatacct tctgcgccca
     7141 cccgctgtaa gccaagcgcc gcctcagcca gaatatcccc ggttttgtcc cactcaccga
     7201 gacgctggca ctcttctatt tcatgaaaat cgacgctatg tagcagcact tgcgcagagt
     7261 gaagcccacc aagccgctgt ttaatgcctt catttatcag acgatagtaa ggaatagtgg
     7321 attcccagct cattcctccc agcaaaccaa ttgttttcat tcctctctcc tgatgtgtgt
     7381 taccgcaaca aggctgaata aggaacgaga caaacgcctc aacggccagg tgcctaatct
     7441 ctattaacga aaaaagggcc ggatgtacag cacatccggc ccgtgaaatc agacgccgat
     7501 atttctcaac ttttcgcctg ccatcagttt gcgctcgata tgttccagcg tgacattttt
     7561 ggtttccgga atgagccaga aagtgatgcc cacaaacgca atgttcagcg cagtgtagag
     7621 ccagaacgta ccggcagcgc caatgctatc aagcagtgtc aggaaggtcg cgccgataat
     7681 catattcgac acccagtttg tcgtcgttga gcaggtgatg ccaaaatcgc ggcatttcag
     7741 cggctgaatt tcagagcaca agatccacac cactggcgcg gcgctcatcg cataaccggc
     7801 aatacacatc atcgtcatgc caacagagag ccaggacaag ccactggaag ccgtaccgtt
     7861 atcaaactgc atcaggcagt aacccagcac cagagtgcct aacgccatca cgctgaaacc
     7921 aattttcagc gccggcttac gccctgcttt atctaccgta aagaccgcaa tgaaggtagc
     7981 gaacataaag gtcagcccca cgaccagagt cgcaatcatc tgttgttctg tggtcgtaaa
     8041 gcccgccatt ttgaagatac gtggcgcgta gtacatgatg atgttcatac cggtaaactg
     8101 ctgcatcgcc tgcaacaaca taccgagaaa tacagcacga cggacgttac ggttgatctt
     8161 aaacaatgcc caaccgccct gttttaactt caggctttca cgaatttcgt tgagttcttc
     8221 tcgcgctttt tccgacgtat cgcgcagcat acgcaatact tcttccgcct caatatgacg
     8281 ccccttttcc gccagccagc gcgggctatt tggcaggaag accaccagaa taatcagcag
     8341 aactgctggt aaagcaagaa cccccaacat tgcgcgccag ttaccgctat aactgaacgc
     8401 tgtatcggat aaaaacgcca gcacgatgcc gagagtgacc atcaactggt acatgctgat
     8461 catcttaccg cgaacgtttt cacttgccat ttcagaaaga tatagaggag cggtgtaaga
     8521 cgcgatcccg acagcaatgc ccaacaccac acgagcggcg attaacatct ctacgctggt
     8581 cgcaaaagcg gaccctatcg aaccgagtac aaacaggatg gcccccgcca tcaggctgta
     8641 tttacgcccc aggcggaacg acagccaacc attaaacagc gcaccaattg ctgcgccgag
     8701 catcatgcta ctgaccaccc attcctgcaa acgactggtc agcacaaagt gatcggtaat
     8761 gaacggcaac gctccggcga ttacgccgat atcaagacca aataacaatc ctgcgaccgc
     8821 agcagctacc gaaacaaaca tattcatacg ccgcgtatcc cgcagaggac gtggcgttaa
     8881 agcagattcc gtattgatag taaccatttt ttcctgccag cagagagtaa gacatagtga
     8941 aaaaatacgt gaacaattca cacaggtgtc aggtcggaaa tagcataaat atggattaaa
     9001 ttgctacgac atgtcgttat gtgatggata ttccaatttt caaattaagt tgaattattg
     9061 agattattat taaccacct
//
LOCUS       DAWDRC010000084         9017 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3841_length_9017_cov_2.572640, whole genome shotgun sequence.
ACCESSION   DAWDRC010000084 DAWDRC010000000
VERSION     DAWDRC010000084.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3841_length_9017_cov_2.572640"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4371..4380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4781..4790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2175 a         2217 c         2227 g         2378 t
ORIGIN      
        1 ccagcgtttc tgataaagcc cgggcgattg gcggcggcgt acttgaggcg ttaatgatgt
       61 tgatgcatca gggcgttaaa gcgacaaaag aagtgctgga agtggcttaa atgaaaatgg
      121 ccggatggta ttacccatcc ggcctttaaa ttactgacaa atcaccgttc cggttttgcc
      181 ttcaatccct tctttcgctt tgctcaatac ggtaatcagt gcttcacgac ctgcgcgaga
      241 gcgagcaaac gaggcggcag cttccacttt aggcagcata gaacctttcg caaaatgacc
      301 ttcttcgata aagcgttcag catcacttaa cgacaggcga tcgagccact gttcattctc
      361 ttttccgaag ttaatggcca ctttttctac cgcagtcaga atgatcagca tatcggcatc
      421 gatcatttct gctaaacggg cgctggccca gtctttatcg ataaccgcgc tggcaccacg
      481 cagatggtta ccttcacgaa taacagggat accaccaccg ccaacggtga tcaccacctg
      541 tccggcatct accagagctt taaccgtttc tttttcaatg atatcaaccg gttttggcga
      601 agcaacgaca cgacgatagc cgcgacccgc atcttctttc agagtgtaac cctgttttgt
      661 cagtagctct gcttcttgct cggtaaagaa cgaaccaatc ggcttggtcg ggttgaggaa
      721 tgccggatcg ttagcatcga cttcgacctg ggtaaccagc gttgctacag gtttattaat
      781 gccacgagaa agcagttctt ctcttaaggc gttttgtaga tcgtaaccaa tataaccctg
      841 gcttaaggca acacagacag acatcggcag cattggcgag tgcgcttcag tttttgctgc
      901 agcttcaaag gcctggttaa tcatgcccac ctgtggcccg ttgccatgag tgacgatgac
      961 ctcatgtccc tgggcaatta aatcaacaat tgcctgagaa gtaattttta ccgctttcat
     1021 ttgcccagcc aggtcatcgc ccagcgcatt cccgcccagg gcgagaacaa ttttcttact
     1081 catttttcct cactcatcat tcttgattgt gtatagatcc ctggcagtgg ttagagcacg
     1141 ggagggacaa acggtttgcg tcgcaggaaa cgacctcgcc cggctttgcc tgtaaaggtg
     1201 ccgtcgcaga aaatcgtttc accacgagat aaggttctga caatcgcgcc ctgacaggta
     1261 aaaccctccc atggcgagta gtcagcgttg tcgtggagat ggcgatgctg aatttgttgg
     1321 ctctgacgtg ggtctataat caccacgtcg ccatcggaac cgggcgctaa taatcctttt
     1381 tgtggccaca ggccaaacag cctggcgggc attgcactgg ttaactcaac aaaacgctcc
     1441 ggtgagatac gtcccgtcat cacgccactg gagaacagca gttgcatgcg gttctccaca
     1501 ccgggtaagc catttgggca gcggctgaaa tcgcctttag aaatttgctg gcgctgggcc
     1561 atcgagaagg tgcagtgatc ggttgccacc acgtcaatcg caccatcact gatgccacac
     1621 cacagtttgt cctgctcgcg tacgttacgc agcggtgggc taagaatgaa cttcatgcca
     1681 tcttctgtat cgtaactgcg ttcgtccaac aggagatatt gtgggcaggt ttcaacccag
     1741 actggctggt gattcgcacg ggcaagacgc agataatcca gacctaagcc gttagacagg
     1801 tgcacgatat agagcggggc gttaccggca atttgtgcca ggttaatcat gcgggcgatg
     1861 gcttccgctt cgcattccag aggtcgactc aaggcatgat aacgcggcgc ggttaaccct
     1921 gcggcgataa actccgcccg cttgctggcg atagctgcgt cattttccgg gtgtacggtg
     1981 gtcagcgcgc cggactcatg caagcggcgt aatgcctgca acacttcatc atcgttgagt
     2041 ttatattgat aggttaaata gagtttaaaa ctgctcagtc cttcctcaac catcatcgga
     2101 atttcgtcga ggatagcgtg attaatatgt tgaatgacac cgtgaaagct gtagtcgatg
     2161 actgctttgt gggcggcata accgcgataa acttccagtt gatggcgtaa ccggcagcca
     2221 tttgggccaa atcccatatg gtcaataatg gttgttgtac cgccacacgc agcagcgcgg
     2281 gtaccggtaa aaaaatcatc acaactgcgc gcgatgccga catcaatatt gaaatgcgta
     2341 tggacatcca cgccgccagg gaaaacgtaa cagccagagg catcaatttc ttcgcaaggg
     2401 agttgcggcg aaatatcggt acccaactgg cgaacaatcc cgctttcaat cagcaaatcc
     2461 tgcttcgcct gtccatctgc attaacaaca atgccgtttt ttatcaatac gcgcatagca
     2521 aactccaaaa accctccggt aaaccggagg gggaataagt gaagagtaat tattcggttg
     2581 ccagccagct taatgggatt gcagcgtaca tagcggcaca agttaccagg tgagatttcc
     2641 aggttttttc gtttggcgcg tgcgcttcag gttctttacc agggccaaaa ccgatgaccg
     2701 gaatgccgtg gcgacccatg atggatacgc cgttagttga gaaggtccac ttatcaacga
     2761 caggcgcttt accaaacagc ccttcataag cattcaccag tgctttaacg gtgaagtgat
     2821 cttcttccac tttccaggtc gggaagtagc attcggttgg ataaaccagg ccagtccagg
     2881 acggacggtc gtagttgtac atagaaacga cagcattcgc tttttgtact gcaggcaggg
     2941 cacggatctc gtccagcgcg ccttcccatg tttcgcccca agtgagacgg cggtcaatag
     3001 aaactgcgca gctatctgcg acagcgcaac gacttgggga ggtgaagaaa atttcggaaa
     3061 cagtcagcgt acctttgccg aggaattcgt catagcccag acgttgagac agttcctgta
     3121 attcgccaag aattggaccc attttgaaaa tggcgttatc accacgttct ggtgccgaac
     3181 cgtggcagct aacaccctga acatcaatgc gaatttccat acgaccgcgt tgaccacggt
     3241 atacctggca gtcggttggt tcggtactga ccacaaattc cgggcgaatg ccggattgtt
     3301 caataatgta ttgccagcac agaccgtcgc agtcttcttc ctgcacagta ccggtaacca
     3361 gcagggtata ttcatcttcc agaccgaggt ctttaatgat tttaccggca taaaccatag
     3421 atgccatgcc gccttcctgg tcggaagtac cgcgaccacc gatcagctcg tcggtttcca
     3481 tgccttcata cggatcgaag tcccagtttt tgatgttgcc aatgccgacg gtgtcgatat
     3541 gagcgtccat tgccaccaga cgcgggccgt ggccgatata accgagaacg ttgcccattg
     3601 ggtcgatttc aactttatcg aagccgactt tttccatctc ttctttaata cgatgtacta
     3661 cgcgtttctc gtcgcagctt tcactgggaa tagcaaccat atcgcgcagg aagcgagtca
     3721 tgtctgcctg gtaatctttt gctttttcaa gaatcagttt gaatggaata ttcttagcca
     3781 ttatttttct ccagattttt tactccccgt tttgcaggga gcagttccaa tagggtgatt
     3841 aaggtgctac agcgtgtttg ccttcccaga caacttcgcg gtagtgcttc acgtcggtgt
     3901 cgccttcagt gctgataacc agcaccacgg catctttgtt cagcgccagt ttttccatca
     3961 ggctttgacg ttgcgggtga taatgaaccg ctgcgagaac gcccaaaccg acagcgccgg
     4021 attcaccgga gatgatgcgc gggtcgttac cgtacggatt acccagcacg cgcataccta
     4081 atgcggcaac gctgtcctgg caagagatga attgggtggc gcagttacgt aggatttccc
     4141 agcccagcgg gttaggttcg ccacaggcca ggcccgccat gatggtggcc atatcaccgc
     4201 caacgttgac aatgtcgcct ttgacgccgg agcgataaat acagtcagct ttgtcaggtt
     4261 caacaataat gctgtgcaga ttttgcgggc tatagacgtc gaccagataa cccagcacac
     4321 caccggccat tgcaccgaca ccggcttgca gcagaacgtg tgtcggggtg nnnnnnnnnn
     4381 cccatttcac gcatttgctc gacggcttca tccgccagtg ttgcgtagcc ttgcatgatc
     4441 caggttggga ttttggtgta gccttcccat gccgtgtcct gtaccacttc ccagccgtgc
     4501 tgctgcgcgt gttgcatggt caggcgaacg gtatcgtcat agttcatatc cgtgacgatg
     4561 cactcggcac cgaggtttag aatggcgtca acgcgttcct gagcagaacc tttcggcatg
     4621 taaatcaccg cattctgtcc gagttgctgt gctgcccatg ccacaccgcg cccgtggttg
     4681 ccgtcggtgg tagtcgcgaa agtcattttt tcgccgatgg catttttcag gtgctcaaat
     4741 gacagcgttt cgatatcaag atgatatttt tcacacaata nnnnnnnnnn ccagttttgc
     4801 ctggctttgc gaaaaaagcg gtgatgtttc gccgttgaaa aatttgttat cggcgatatc
     4861 aatcttcaat gagaaaacgg acatatccta tccttttggc ctctggaaat agagggccga
     4921 ccagctgatc ggcccgaggg gttatttcac gcgttcttgc gcttctttca gcagttgctc
     4981 gagcagtgca cctggtttgg cgtatttacg ggacaggatc atcgcggcga tgatataagg
     5041 cttccagctg gcttctttgt aggtagcgat acggtatttt tcgaatacgc cttcagtcac
     5101 ttcaccttct ttgcaggata cgccgctgat atcagctggc aggcagtgca tgtacagggc
     5161 ttcgccatca cgggtcagtt ccatcatctc ttcagtacaa tgccagtctt tgtgttgcgc
     5221 gttctgtgcc agacactgtt tttccagtgc ttttaagcct tcgtgatcgt tcgcacgcag
     5281 caattcagta cgctcttcca tcactttgta aggtgcccat gacttcggat agacgatctc
     5341 tgcgtctttg aaggcttctt ccatgctggt gacctgacgg aagctaccac cggaggcttt
     5401 agcattgttt ttagcaactt caacgacatc cgggatcagg tcgtagcctt ccggatgggc
     5461 cagggtgaca tccataccga agcgggtcat cagaccgatg atgccttgtg gtacagagag
     5521 cggtttgcca tagcttgggg agtaggccca ggtcatggcg atttttttac ctttcaggtt
     5581 ttccagtgaa ccaaagtgtt cacgtaacca tgccagatca gccattgatt gagtcgggtg
     5641 gtcaatatcg cattgcaggt tcactaaagc cggacgctgt ggcagaaccc cttgtttgta
     5701 gccgtcatca agtgccgcgc caacttcacg catataggcg ttgcctgcgc ccagatacat
     5761 atcgtcgcga ataccgatag cgtcagcgca gaaggagatc atattggcgg tttcacgcac
     5821 ggtttcgccg tgagcgattt gtgatttacc ttcgtcaaga tcttgttgtg caaggccgag
     5881 caggttaagc gcggaagcat aagagaagcg ggtacgggtg gaattgtcgc ggaatacgga
     5941 aatacctaat ccactattaa agactttggt tgagatgttt tcagcacgca gtgcttttaa
     6001 cgcggcggca acgtccagta cttgtttcag ttcgtctggc gtctgttccc acgttaacaa
     6061 aaaatctttt tcatgcaggt gagaggtcag cgaattgata tccttaatca gctcattaac
     6121 agttttcatc ataaagtccc gatagtagaa tgacaggcaa agtggtttat ttcactgtga
     6181 cgtgccaaag tgattggtgt tttcacagct atacctggga ctaacaataa gtgtgccaga
     6241 attatccacg cggctgcgaa aggctaattt gacggataat gtgtggggtt gatcacatat
     6301 tgatgccagt ttccggttgt ttgtaaaata cgaaaacgtt tggctttttt tatcacgtta
     6361 tttatttgct gctaagtatc agaaataaga taaagaggca ggctatctct cttcagtttg
     6421 ataaacttgc cgctgtaatt ctcatattga tatagaaatg tgatatctct tccataattt
     6481 tattagaaag tgataaaaat cttatgtttt tatcaaagcc aatttcgcct gagttaatat
     6541 catcatgatc tgtgaaccat caacgtcttc aatgatcgac ttttaagtaa gtctggaagg
     6601 taaagctatg gagcttgcga ctacgcagtc agtattgatg caaattcaac caacaattca
     6661 gcgttttgcc agaatgcttg ccagcgtttt gcagcttgag gttgagatcg ttgatgaaaa
     6721 cttgtgtcgc gtcgccggaa cgggcgcgta tgggaagttt cttggccgcc agttgagcgg
     6781 caactcacgc ctgctccgcc acgtcctgga aacgaaaact gaaaaagttg tgacacagtc
     6841 tcgcttcgat cccctttgtg aaggttgcga tagtaaagaa aattgccgcg aaaaagcatt
     6901 tctgggtacg cctgtcattt tacaggatcg ttgtgttggg gtgataagtt tgattgccgt
     6961 tacccacgag caacaagaac atatcagtga taatttacgc gaattttccg attacgttcg
     7021 ccatatatcc accatttttg tttctaaact tctggaggat cagggaccag gagataacat
     7081 cagtaaaata ttcgcgacca tgatcgataa tatggatcag ggcgtgttag tggttgatgc
     7141 tgataatcgg gttcagtttg ttaatcagac agctttaaaa acactcggtg ttgtacaaaa
     7201 taatattatt ggaaaacctg ttcgtttcag accattaaca tttgagagta attttaccca
     7261 tggtcatatg caacatattg tttcgtggga tgataaaagt gaattaatca ttggtcagtt
     7321 gcataacatc cagggccgac aactattttt aatggcgttt caccaatcgc ataccagttt
     7381 ttctgtagca aatgcacctg atgaaccgca tattgaacaa ttggttggcg agtgccgtgt
     7441 tatgcggcaa ttaaaacgac tcattagccg tattgcaccc agcccatcca gcgttatggt
     7501 ggttggtgaa agcggcacgg gtaaagaagt cgtcgcccgt gcaatccata agttgagcgg
     7561 aagacggaat aaacccttta ttgctatcaa ctgtgccgcg attccggaac agcttctgga
     7621 gagcgaactg ttcggttatg ttaaaggcgc atttactggc gcttctgcca acggtaaaac
     7681 agggttgatt caggcggcga atacgggcac gctgtttctc gatgaaattg gtgatatgcc
     7741 attaatgttg caggctaaat tactgcgcgc tattgaggcg cgtgaaattc tgccgattgg
     7801 tgccagtagc ccaatacaag tcgacatccg catcatttct gcaactaatc agaatttggc
     7861 ccagttcatt gccgaaggta aattccgcga agatctcttc taccgactta atgttatccc
     7921 gataactctt ccaccgctgc gtgaacgtca ggaagatatt gaattactgg tgcattactt
     7981 tttacatctg catacccgtc gtctgggatc ggtttatcct ggcattgctc ctgatgtcgt
     8041 cgaaatattg cgtaagcatc gttggcccgg aaacctgcgc gagttaagca atttgatgga
     8101 atatctggtt aacgttgttc cttcaggtga agttatcgac agcacgctat tgccgccaaa
     8161 tctgctgaat aatggcacaa cggagcaaag tgatgtaaca gaggtcagtg aggcgcacct
     8221 gtcactcgat gatgcgggcg gcacggcgct ggaggagatg gaaaagcaaa tgatccgcga
     8281 ggcgctttca cgtcataaca gcaagaagga agttgctgat gaactgggca tcggcattgc
     8341 tacgctctat cgcaagatta agaaatatga gttgttaaac acataacctc tgagaatccg
     8401 gcctgaattc aggccggata tccttttact tcgttttctc gcaatccaga actgtattta
     8461 caatcatctg ataccccgtg cagcgacaaa gatttcccgc cagtccacga cgaatttccg
     8521 taatggttaa tggtttttcg cgtggttttg ccagcatcgc cgtggtagcc ataatcaggc
     8581 ccggcgtaca aaacccgcat tgcactgcgc cggatttcgc ataagccagt tggacatgag
     8641 aaagtttacc gcctttcgct tcaccttcca gcgtgcggat ctcttttcct tcagcccagg
     8701 ccgcaaggaa taagcaactg tctattgctg tgccgtcgac cagcaccgta caggcaccac
     8761 attcacccac gcagcaccct tgtttgacac ttagcagtcc ttgttcgcgg agtaattccg
     8821 agagcggcat tcctggcgcg gcgtgaagct gaaaaggcat cccgttaatg gtgcattcga
     8881 tggtaattgt ttcgctgtga ttcattgcaa ttttcccccc gccgcggcga cggcttcgct
     8941 aatcactttt ttggtcatcg tctggatgag atgcagacga aactctttac tggcccgcca
     9001 tgaagaacgc ggggtga
//
LOCUS       DAWDRC010000085         8978 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3858_length_8978_cov_2.750196, whole genome shotgun sequence.
ACCESSION   DAWDRC010000085 DAWDRC010000000
VERSION     DAWDRC010000085.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3858_length_8978_cov_2.750196"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1647..1656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2921..2930
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2150 a         2405 c         2310 g         2093 t
ORIGIN      
        1 cggttttgat gaggcaaaac tgtttatcac cgcagcacct cctttacctg gcgttcaacc
       61 tcatcaacac gcgcttcgtc cacgcgatcg gctttggtca gcgccactgt cagcatcggg
      121 ttaccggtca gctgcaaaat cgccagatgc tcacgggtct gtgccatcac accgtcatcg
      181 cacgccacca ccaacagcgc gtgatcgata ccaccaacgc ccgccagcat gttggaaaga
      241 aacttttcat gaccgggtac gtcgataaaa ccaggcacgc gaccatccgg ctgcggccag
      301 taggcatagc cgagatcgat ggtcatgccg cgcttttttt cttccggcag acggtcggca
      361 tttacgcccg taatcgcctg caacaaggtt gttttgccgt ggtcaacgtg tccggcagtc
      421 gcaataatca tttcaacaac atctccaaaa accgttgctc atcttcaagg cagcgtaaat
      481 ccagccacaa tcgtccgtca taaatacgac caatcaccgg cactggcaat tcacgccagc
      541 gggcggctaa tgactcaagg tggctaccgc gtccatcatg gggggtaaac gttaatgccg
      601 cgctcggcag gcgatcaacc ggcagcgaac cactgccgat ctgcgaaaga catggcataa
      661 cctgtaccgc aaactccgcg ccgtaatgtg cggcaagggg agcctgtaaa cgttgtgcct
      721 ggatttgaat gacctctgcg ctgcgggtaa gcaggcgcag ggtcggtaat ttttcactca
      781 gagcttcagg gtgtaaataa agacgcaacg tggcttccag cgccgcgagg gtcattttat
      841 ccgcgcgtaa tgcacgcttc agcgggtggc tttgcaggcg ggcgatcatt tcttttttac
      901 caacaataat tcctgcctgc ggcccgccta acagcttgtc gccggagaag ctcaccagac
      961 tgacgcccgc cgcaatcaac tcctgcggca ttggctcttt cggcaaaccg tactggctaa
     1021 gatcgaccag cgagccactg cctaaatcag tcaccactgg aacatccagt tctttgccga
     1081 gcgccaccag ttccgcttca tctatcgctt tggtgaaccc ctgaatgctg tagttactgg
     1141 tatgtacttt catcaacagt gcggtatttt cattcaccgc ctgacgataa tcattcgcgt
     1201 gcgtgcggtt ggtggtccct acttcgtgta gggtgcatcc tgcctgacgc ataacatcgg
     1261 gaatacgaaa cgcgccgcca atctccacca gttcgccgcg agaaaccacc acctctttcc
     1321 cgctggcagt ggccgccaac atcaataaca ccgccgccgc attgttatta acgatacagg
     1381 catcttccgc ccccgtaata cggcacagca gctgcgccag cgcccgatcg cgatgtccgc
     1441 gtccggcgtc gtccagatcg tactcgaggg tcactggcga acgcatagcc ttcgtaacgg
     1501 cttccaccgc ggcttccgcc tgtaaagctc gcccaaggtt ggtatgcagc acggttcctg
     1561 tcaggttgat taccggacgc agcgcgcttt gcgcctcttt cgtcaaccgg gcatcgactt
     1621 cttgcgccca gttttcacac cacgcgnnnn nnnnnnccac gcgggcagcg tctggctgtc
     1681 acgaatcact tctcgcgctt cgtcaagcat ctgacgcagc aattccacca cgcgggtgtg
     1741 accataagta tcacgcaaag aaaggaagga gctatcgcgc aataagcgat caatagccgg
     1801 aagttgacta tagagggaac gcgtttcggt tgtcatagga aacctggctg atcaaggccc
     1861 tctcacacgg agaagggcgt ttaacataac cacggattgt aacgtgagat gggtcaggag
     1921 gacatatcgc gcatcaagcc tttggcggtt cggtgcgagc aaaactttcg cggctaaaca
     1981 ggttttcgac cagtttgact aagtgtgggc ggtcgacaca caagcctggc gcaacgcggc
     2041 ggaaattgag atatccgacg gcacaggcaa tggcgatagt cgccagattg accgtatcgg
     2101 ttttgagcgt gccatcgacc agatatcctt ccagcacatc cagactgcgg ttgattttct
     2161 cacgctgacg taacaattca tcttcagact gctgcgccgc cggacgcgcc tgttcacgca
     2221 ccgataccag cccggcatcc ataatgccat ccgccagtgc ctcaattttg cgcacccgca
     2281 acgactccag cggatcgcgc ggcaacatcg ccggagcaac attcattaat tcaatatatt
     2341 cagcgatgat cggtgaatca aaccagcatt cgccctcttc ggtcaccagc accggcactt
     2401 ttcctaacgg gttaaattgc gccacgccgt tatccgcgtt atagggcagt tcattaataa
     2461 attcgaacgt tatgcccctt tctaacaaca gaatagaaag tttgcgtaca aacgggctgg
     2521 tgtagctacc gacgagtttc atgccgagtc ctttgtgcga ggaaaaatat cagtatggct
     2581 caggctcgcg caaaacacag gtgatacaag tctcaatgat ccaaataaag ataatgcatc
     2641 caactggtca ttcttagcag cactttccgc atcaccgaaa catggctgaa atggtcggag
     2701 taaactgcca cctgggcgta gatgccgtcg ggtaaggcat cgatatcagc gttagggtcc
     2761 agttcaatgg ttcccagcac accgtccgtg ccgggcacga ccgttaatga ttgcaatacc
     2821 ccctgcgcct gataagaacc gcctggcacg acaggtaaaa tactggtcag tttgccgtga
     2881 aacacctgcc caggtagcgc gttaaacacc acttccgcat nnnnnnnnnn cgagttttgc
     2941 cgaaattggg cgacaatttg ccgtttttgc tcggggatga agaccatcac cggacgcagc
     3001 ggcaaggcag cggcgtatgt acctgggcgg atcagtacct gagtgacgta gccattgctc
     3061 ggtgcgcgaa tgacggtctg ctcaaggtta tattttgctt cagtaagttg cgctcttaag
     3121 ctcacaatct gcgattgctc gccgttaacc atactgtcga gctggctctg gatctgcgcc
     3181 tgctccgcca ccgagccttt caccagcgca tcctgcgcga ggaaattttg ccgcgcatcg
     3241 tcgatgtcac gttccgagaa cggattcacc gccgcctggc tgcctttcag ataacgttga
     3301 taatttttaa acagacggtc gcgctccgct gaaacctggg tggtgttggc ctgcgcttca
     3361 gtgagctgcg cgcgcagcgt ctttatatta tgcgtcgccg tcatcaggtc agcctgaagc
     3421 ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa aaagcacctc gcccttctga
     3481 ataagctgat tatttttgtc ggtgacttca gtaacaattc ccgtcacctg tggcgtgatg
     3541 gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag ggtggttgta gttcatcaac
     3601 aaaatcaaac cactcaccag aaacacgcct cccagcgccg ccgtcgccag cgtccactga
     3661 ttcaccggaa tgcggaagat tttaaagacc gcccacgcca gcgccacgta agttaaaaca
     3721 atcaatagat ccataattag atctccggaa acgtggaata gtcagtcttt ttctcggcgg
     3781 cgagttggtg ctccagccgg gaaatccggt cagaaagtgc ggcgatttcc gggtctggca
     3841 cgtcgccttg cggcgcaaca tgtgactgca tgccccagcc gcgctccggt tgatagagcg
     3901 tcgcccagat ccacaggaac ggccagataa catgcagagt aaacaggctc acccagcccg
     3961 ccgtatgaat agcgtcggca tggggatggt tgcgcttttt ggcaatcaga taagggatgt
     4021 catgtatcgc gatgattcca taaaaaatca ccagaaagac gaagatcagc actcccaacg
     4081 cgaaatagtt tagaaacata tctgcctcgg attcacgttt atcagtgttg ttttttggct
     4141 ggcagccaga agggagtcag gctgatattt tgacaataat ccgggttcgc gattctcgcc
     4201 ataacaccaa agaataattt taagaggtga tgagttgctt agttacataa cgatttcatg
     4261 acgaaggcat aacatgctgt agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga
     4321 ttcagatcac aaagattcaa caaatcatca aaataaaaat gtgacattac tcacatttaa
     4381 atgccatttt tagcgaaaat cgccgccttg ttgctttttt acacaagcat tttgtgatga
     4441 gcgtcacgtc aattacctct ctacccccta catttatgtg actgatatca cacaaaaggc
     4501 cgccgactgg acagttaacc gattcagtgc cagatttcgc agtatctaca aggtccggct
     4561 acctctgccg ccacattaac aaaaaacctc gggcttccag cctgcgcgac agcaaacata
     4621 agaaggggtg tttttatgtc atccgatatt aagatcaaag tgcaaagctt tggtcgtttc
     4681 ctcagcaaca tggtgatgcc aaatatcggc gcgtttatcg cgtggggtat catcaccgcg
     4741 ttatttattc caacagggtg gttaccgaac gaaacgctgg cgaagctggt cgggccgatg
     4801 atcacttatc tcctgccgct gctgatcggt tataccggtg gtaagctggt aggcggcgaa
     4861 cgtggcggcg tagtcggtgc catcaccacc atgggcgtta tcgtcggcgc agacaggccg
     4921 atgttcctcg gctctatgat tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac
     4981 cgctgggtag acggtaagat caaatccggt tttgaaatgc tggtgaataa cttctccgca
     5041 ggcatcatcg ggatgatcct cgctattctg gcatttctcg gcattggccc ggttgttgaa
     5101 gccctgtcta aaatgctggc ggcgggcgtt aacttcatgg ttgtccatga catgctgccg
     5161 ctggcgtcta tctttgttga accggcgaaa atcctgttcc tcaacaacgc cattaaccac
     5221 ggtatcttct cgccgctggg tattcagcag tcccatgaac tgggtaaatc catcttcttc
     5281 ctgattgaag ctaacccagg tccgggtatg ggcgtgctgc tggcgtacat gttctttggt
     5341 cgtggtagcg ctaaacagtc tgcgggcggt gcggcaatca tccacttcct gggcggtatc
     5401 cacgaaatct acttcccgta tgtgctgatg aatccgcgtc tgattctggc cgttatcctc
     5461 ggtggtatga ctggcgtgtt tacgctgact atcctgggcg gtggtctggt ttctccggca
     5521 tctccgggtt ctatccttgc tgtactggcg atgacgccaa aaggtgctta cttcgctaac
     5581 atcgcgggtg tgtgtgcggc gatggcagtc tccttcgttg tctctgccat tctgctgaaa
     5641 accagcaaag tgaaagaaga agatgatatt gaagctgctg cacgtcgcgt tcaggaaatg
     5701 aaagctgagt ctaaaggcgc atctccgctg tctgcgggcg atgtgactaa cgacctgagc
     5761 cacgtacgta aaatcatcgt tgcctgtgat gccggtatgg gttccagtgc gatgggcgca
     5821 ggcgtgctgc gtaagaaaat tcaggatgca ggtctgtcgc agatttcggt cactaacagc
     5881 gcgatcaaca acctgccgcc agatgtggac ctggtcatca ctcaccgtga cctgaccgaa
     5941 cgcgctatgc gccaggttcc gcaggcacag catatttcgc tgaccaactt cctcgacagc
     6001 ggcctgtaca ccagcctgac cgaacgtctg gttgctgccc agcgccatac tgaaaacgaa
     6061 gtgaaagtga aagacagtct gaaagacagc tttgacgatt ccagtgctaa tctgttcaaa
     6121 ctgggcgcgg agaacatctt cctcggtcgc aaagcggcca ccaaagaaga agcgattcgt
     6181 tttgctggcg agcagctggt gaaaggcggt tacgttgagc cggaatacgt tcaggcgatg
     6241 ttggatcgtg aaaaactgac cccgacttat ctgggtgagt ctatcgcggt gccacacggt
     6301 acggttgaag cgaaagatcg cgtactgaaa acgggcgtcg tgttctgcca gtacccggaa
     6361 ggcgtgcgct tcggtgaaga agaagatgac attgcccgtc tggtgattgg tattgctgcc
     6421 cgtaacaacg agcacattca ggtgatcacc agcctgacca acgcactgga tgatgagtcc
     6481 gttatcgagc gtctggcgca caccaccagc gtggatgaag tgctggaact gctggcaggt
     6541 cgtaagtaat ccattcccac cctctccgca tgaagaaggt ggggctaatt gcctgatgcg
     6601 cttcgcttat caggcctaca acataaagca caatttgttt tgtcggtcgg ataaggtgtt
     6661 tacgccgcat ccggcagtcg gtacacaatg cctgatgcga cgctgacgcg tcttatcagg
     6721 cctacaggtc agtgccaaac acgtaggccg gataaggcgt ttacgccgca tccggcaaaa
     6781 agcccctctc ctcacggaga gggtttgggt gagggaaaag cctcacccca gccctctcgg
     6841 gtaaaaacat tgatgaaggt taatactatg aaagcattac attttggcgc aggtaatatc
     6901 ggtcgtggct ttatcggtaa actgctggcg gacgcgggta tccaactgac gtttgccgat
     6961 gtaaatcagg tggtacttga tgccctgaat gcccgtcata gctatcaggt tcatgtggtc
     7021 ggcgaaaccg agcaggtgga taccgtttct ggcgtcgatg ccgtcagcag cattggcgat
     7081 gatgtcgttg atctgattgc tcaggtcgat ttagtcacta ccgccgttgg ccctgtcgtg
     7141 ctggaacgta ttgctccggc catcgccaaa gggctggtga aacgtaaaga acaaggtaat
     7201 gaatccccac tgaacatcat cgcctgtgaa aacatggtcc gcggcaccac gcagctgaaa
     7261 ggccatgtga tgaacgccct gccggaagat gccaaagcgt gggtagaaga acacgttggc
     7321 tttgttgatt ccgccgttga ccgcatcgta ccgccttcgg cttcggcaac taacgatccg
     7381 ctggaagtga cggtagaaac cttcagcgaa tggattgtcg ataaaacgca gttcaaaggc
     7441 acgctgccaa acatccctgg catggaatta accgacaatc tgatggcatt tgtcgaacgt
     7501 aaactcttca ccctgaatac aggtcatgct ataaccgcgt acctcggaaa actggccggt
     7561 catcagacca ttcgtgacgc aattctcgac gagaaaatcc gcgcggtggt aaaaggggca
     7621 atggaagaaa gcggcgcggt actgatcaag cgctacggct ttgatgctga caaacatgcg
     7681 gcatacatcc agaaaatcct cggtcgtttc gagaacccgt acctgaaaga tgatgtagaa
     7741 cgcgtaggcc gtcagccgct gcgtaaactg agtgctggcg accgtctgat caagccactg
     7801 ctcggaaccc tggaatatgg tctgccacat aaaaacctga ttgaaggtat tgccgctgca
     7861 atgcacttcc gcagtgaaga tgacccgcag gctcaggaac tggcagcgct gattgccgac
     7921 aaaggcccac aagccgcgct ggcacagatt tccggtcttg atgccaacag cgaggttgta
     7981 tccgaggcgg taaccgctta taaagcaatg caataatggt ggaccaggcg caggataccc
     8041 tgcgcccgaa taacagattg tcagatatgc aggcaacaat ggaacaaacc caggcctttg
     8101 aaaaccgtgt gcttgagcgt ctgaatgctg gcaaaaccgt gcgaagcttt ctgatcaccg
     8161 ccgtcgagct tttgaccgag gcggtaaatc ttctggtgct tcaggtattc cgcaaagacg
     8221 attacgcggt gaagtatgct gtagaaccgt tactcgacgg cgatggtccg ctgggcgatc
     8281 tttctgtgcg tttaaaactc atttacgggt tgggcgtcat taaccgccag gaatacgaag
     8341 atgcggaact gctgatggca ttgcgtgaag agctaaatca cgacggcaac gagtacgctt
     8401 ttaccgacga cgaaatactt ggaccctttg gtgaactgca ttgcgtggcg gcgttaccac
     8461 cgccgccaca gtttgaacct gcagactcca gtttgtatgc aatgcaaatt cagcgttacc
     8521 aacaggctgt gcgatcaaca atggtccttt cactgacaga gctgatttcc aaaatcagct
     8581 taaaaaaagc ctttcaaaag taagtacttt cgcttacttc tcctctacct gtttcggacg
     8641 ataaagctga cgataatact ccagacgttg tagatataac ggcacgcttt catcaggtat
     8701 tccggacgga atcgcgttac ggggaggtag ttttttcaga tactcccgga acgcctggct
     8761 ggatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac ctaatttgcc
     8821 catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat tttagtttag
     8881 atcccggtat aaatgacatt aaggaaaacg tgctcaatcc tcaataatca cctgcttctg
     8941 ttgactatct tttttcttta aatttcttaa ctaatcaa
//
LOCUS       DAWDRC010000086         8956 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3867_length_8956_cov_2.357713, whole genome shotgun sequence.
ACCESSION   DAWDRC010000086 DAWDRC010000000
VERSION     DAWDRC010000086.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3867_length_8956_cov_2.357713"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    251..293
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    730..739
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4784..4793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5309..5318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2007 a         2237 c         2355 g         2284 t
ORIGIN      
        1 catctcgtca taacccattt tgcaggtacc gcacgggtgg aaggcggttt cggcgtggtt
       61 acgcacgaac tcatcgagct gctcatccgt ctggcattcg acaccggggc tgatttcgcg
      121 gccacgatac tgatccagcg ccggttgatg catgatctcc cgggtgatgc gaattgcgtc
      181 gcggaactcc tgccagtcct gctcgtgcga catgtagtta aacagaatcg ccggatgctg
      241 gtgcgggtcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgctctt
      301 tcactgcgtt cgagccgtta tagttaatcg ccactggcag gaaatggtac tgaatattcg
      361 gccacgcaaa ttcctcacgg ctgcgaataa atccgcctgc ttcaaagtgg ttgctggcac
      421 caacgccggt gccgccaaac agccactccg caccgatttt cggctgattc caccactgca
      481 gggcagggta gagggaaacc ggttctttgc actcatattg cagatacatc tccaggtgat
      541 cctgaagatt ttcgccgacg ccaggtaatt catgcaccag cggaatatca aactccgcca
      601 gcagttcagc gttgccgacg ccggagcgtt gcaggatctg cggtgaagca atcgcgcctg
      661 cacataacag cacttcttta ttcgccgccg cgcgggtcgg aatggtgctg tcgccttcca
      721 gccactcgan nnnnnnnnna ctcgacgccc accgcgcgtt tgccgtcaaa aaagatgtga
      781 tcggtcatgg cgtgagtacg aatggtcagg ttagggcgcg atttggcctg atcgagatag
      841 ccacgcgcgg tgctggcgcg acggccatgc ggcgtgacgg tgcgatccat cgggccaaaa
      901 ccttcctgct gatagccgtt gagatcgtcc gtgcgcgggt agcccgcctg cacgcccgct
      961 tcaatcatcg cttcaaacag cggattgacg ccgggtttgg aggtggtgac gctcaccggg
     1021 ccatcaccgc cgtggtagtc gttctcgccc acatcgcgag tctcggcctt gcgatagtag
     1081 ggcaggcagt cgagataact ccagttctcc agaccaggtt cttgtgccca gttatcgaga
     1141 tccagcgcat tgccacggat gtagcacata ccgttgatca gcgatgaacc gcccagacct
     1201 ttgccgcgtc cgcactccat gcggcggttg ttcataaacg gttcgggttc cgtctcatag
     1261 gcccagttgt agcgtttgcc ctgtagcggg aacgccagcg cggcgggcat ctgggtgcgg
     1321 aagtcaaagc gatagtccgg gccgcctgct tcaagcagca gcacggttgt gttcggatct
     1381 tcagtcagac gggtagcgag aacgttgccg gctgagccgg caccaataat gatgtagtca
     1441 aattgcaaat aaacctcctg gttagaatat ggactggaat ttagccatct caacctggat
     1501 ggacttcacc tgggtgtaac tctggagcgt catcacgccg ttctcgcgac caatgccgga
     1561 gtgtttgtag ccgccaacgg gcatctctgc cggggattcg ccccaggtgt tgatccagca
     1621 aatacccgct tccagctgat gaatgacgcg aagcgcgagg tttaggtccg ccgtcacgat
     1681 acccgccgcc agaccgtagt cggtatcgtt agcgcggcga atgacttcgt cttccgactc
     1741 gtaggtcaga atggacatca ctggcccgaa aatctcttct cgcacaatgg tcatatcgtc
     1801 gctgcaatcg gtgaacacgg tcggtgcaac ccatgcgccg ttatcgaagc tatccccctt
     1861 cagtacatcg ccgccgcaca gtacgcgcgc gccttcctct atgcctttgg cgatatagcg
     1921 cagcacgtta tcgcgatgcg ggaagctgac cagcgggccg aagttagttt gcggatcgaa
     1981 aacgtcgccc gcgcgaatgc gctcaacgcg cgccagaata ttttgttcaa atgcggcttt
     2041 gcatttcgtc ggaacgaaga cgcgagtgcc gttggtacac acctgaccgg agctgaagaa
     2101 attcgccatc atggcgatat cggcggcgag atcgagatct gcatcgtcga aaacgatcag
     2161 cggtgattta ccgcccagct ccatggtcac ttctttcagg gaggaagcag ccgagttagc
     2221 catcactttt ttgccgctgg cgacaccgcc ggtaaatgac actttggcaa tgcccggatg
     2281 atcggtcaga tattgtccgg tctccgcgcc cacgcccggc aacacgttaa atacgccgtc
     2341 cggcaggccc gcttcgctgt aaatttcggc cagctttaac gcggtaagcg gggtgacttc
     2401 gctcggtttg aaaatcattg cattgcctgc cgccagcgct ggggcggatt tccacagggc
     2461 aatctgaatc gggtagttcc atgcgccaat ccctgccact acgcccagcg gttcgcggcg
     2521 ggtataaaca aatgacgtct cacgcaacgg gatctggctg ccttccagcg ccgggatcag
     2581 cccggcgtag tactccagca cgtccgcacc ggtaacgata tcgacggttg aggtttccga
     2641 atatgctttt ccggtgtcaa gggtttccag ttttgcgagt tcgtcattgc gttcacgcag
     2701 aatatcaacg gcccgacgca gaatacgcga gcgctccata gcggtcatcg ccgcccagat
     2761 tttttgccct tgttgggcac tcttcacggc gcgatcgaca tcctcacgcc cggcggcctg
     2821 cacggtcgcc agcacgttac cgttggccgg gttaatggtc tcgaaggtgc gaccgctggt
     2881 ggcggaggta taaccaccat gtatataaag ctgctgttct gccattcggg acatcacgtc
     2941 tcctcggtta atcggtgggt aaatgctgag taataaagtg gcgggtcagc gagtgtgcga
     3001 gaggtttatc cagcggtttg ccactcagtg ccgcgcgcag ccataatccg tcaattagcg
     3061 cggcaaggcc gtagcccgct tcctgtgcct gttggcgcgg taactcgcgg cgaaattcgc
     3121 tcaccagatt cgacaataag cggcggctac taacctgctg caagcgatag agcatcggct
     3181 gatgcatact gctggcccag aaggccagcc aggctttcat cgccgcactg ctcacctggg
     3241 tttcatcgaa gtttccgaca acaatcgcct gtaatcgcag ctctgcactg ccctgcggaa
     3301 gtgcatgtaa tcgattcaaa accgcgtcac gcagctgact ggtgatatcg cgcatggttg
     3361 cttccagcag accatttttg tccctgaaat agtgactgat gatccccgta gaaacgcctg
     3421 cacggcgggc gatctgcgcg atcgttgcat cgtgcatgcc cacttcattt attgcttcca
     3481 gcgtggcgtc gatcagttgt ctgcgccgga tcgactgcat ccccaatttg ggcattttcg
     3541 ccactccatt catcagcgtt gtttatctat taaagctgtt attgattgga cgttcaatat
     3601 aaaatgtgtc ttaattgtta cgaatttgat tttaaatggt aacaataaca gtggggatac
     3661 tggatgacag acctttcaca cagcagggaa aaggacaaaa tcaatccggt ggtgttttac
     3721 acttccgccg gactgatttt gttgttttcc ctgacaacga tcctgtttcg cgacttctcg
     3781 gccctgtgga ttggacgcac gctggactgg gtctctaaaa ccttcggttg gtactatctg
     3841 ctggcggcaa cgctctatat tgtctttgtg gtctgtatcg cttgttcgcg ttttggttcg
     3901 gtgaagctcg ggccagaaca atccaaaccg gaattcagtc tgctgagttg ggcggcgatg
     3961 ctgtttgctg ccgggatcgg tatcgacctg atgttcttct ccgtagccga accggtaacg
     4021 cagtatatgc agccgccgga aggcgcggga cagacgattg aggccgcgcg tcaggcgatg
     4081 gtctggacgc tgtttcacta cggcttaacc ggctggtcga tgtatgcgct gatgggcatg
     4141 gcgctcggat actttagcta tcgttataat ttgccgctca ctatccgctc ggcgctgtac
     4201 ccgatcttcg gtaaacggat taacgggccg ataggtcact cagtggatat tgctgcggtg
     4261 atcggcacca tcttcggtat tgccactacg ctcggtatcg gtgtggtgca gcttaactat
     4321 ggcttgagcg tactgtttga tattcccgat tcgatggcgg cgaaagcggc actgatcgcc
     4381 ttgtcggtga tcatcgccac tatctcggtg acatccggtg ttgataaggg cattcgcgtg
     4441 ttatcggaac tcaacgttgc gctggcgctg ggattgatcc tgttcgtgtt gtttatgggc
     4501 gacacctcgt tcctgcttaa tgcactggtg ctgaatgttg gcgactatgt gaatcgcttt
     4561 atgagcatga cgctcaacag ttttgccttc gaccgtccgg ttgagtggat gaataactgg
     4621 acgctcttct tctgggcatg gtgggtggca tggtcgccgt ttgtcggctt gttcctggcg
     4681 cgtatctcgc gtgggcgtac cattcgccag ttcgtgctgg gcacgttgat tattccgttt
     4741 accttcacgc tgttatggct ctcggtattc ggcaatagcg cgcnnnnnnn nnncgctgta
     4801 tgaaatcatc cacggcggcg cggcatttgc cgaggaagcg atggtccatc cggagcgcgg
     4861 cttctacagc ctgctggcgc agtatccggc gtttaccttt agcgcctccg tcgccaccat
     4921 tactggcctg ctattttatg tgacctcggc ggactccggt gcgttggtgc tggggaattt
     4981 cacctcgcag cttaaagata tcaacagcga cgcccccggc tggctgcgcg tcttctggtc
     5041 ggtggcgatt ggcctgctga cgctaggcat gctgatgacc aacgggatat ccgcgctgca
     5101 aaacaccacg gtgattatgg gcctgccgtt cagctttgtg atcttcttcg tgatggcggg
     5161 gttgtataaa tctctgaagg tagaagatta ccgccgtgaa agtgccaacc gcgataccgc
     5221 accgcgaccg ctggggcttc aggatcgcct gagctggaaa aaacgtctct cgcgcctgat
     5281 gaattatccg ggcacgcgtt acactaaann nnnnnnnntg gagacggtct gttatccggc
     5341 aatggaagaa gtggcgcagg aactgcggtt gcgcggcgcg tacgtggagc taaaaaacct
     5401 gccgccggaa gagggacaac agttggggca tctggatttg ttggtgcata tgggcgaaga
     5461 gcagaacttt gtctatcaga tttggccgca gcaatattca gtgccgggct ttacctaccg
     5521 cgcacgtagc gggaaatcga cctactaccg gctggaaacc ttcctgttag aaggcagcca
     5581 gggcaacgac ctgatggact acagcaaaga gcaggtgatc accgatattc tcgaccagta
     5641 cgagcggcac cttaacttta tccatctcca tcgtgaagcg ccgggccata gcgtgatgtt
     5701 cccggacgcg tgattgttat tgcataaaac cgcgccatgt ctgcatatgg cgcggtttct
     5761 cttacctctt gatacgttat atctgtacag ttaagccctt agtatcgatt gatgattgcc
     5821 aggcaacaga taataaaaaa gaaaagaact attgaagccc aaaaactacg tttggactgt
     5881 tgtgaatgtt caataagctt agtcttattt aatgtaaata ttggtgctaa tttgaaatga
     5941 cgatttattc atggggaacg cgtagcgcaa atcaactaaa atcaatgtaa ccgtttgttg
     6001 tttaaggttt aattgtctgt gtctgatatt ttattgattg ttttaaatat tgtttttatt
     6061 gtcattgtta taatgttggc tatctattgc tgaatggatt cagtcttaag aagcaggttg
     6121 ttaagggata ggcatagagt aatgacgtgt atgcataacc aacatcttta ctcattatgt
     6181 cattgaatgt tgacgctatg tgtttatgag gttgaggtat tttcagttga tctggattgt
     6241 taaattcata taatgcgcct ttactcatga atgggcgtca atacgtagtg ggaaattata
     6301 aatatcgaaa tagtccaact acttctttat taccaaaaat gagtatctga attttaatat
     6361 tgcattcttg cgtgattatc tcctgagttt gacttgtgat taccttttta aggtatttag
     6421 cgtaactgtt tttgagcgag catcagaggt aaagataatc ttcttgatag tgatgtggga
     6481 tgttatacgt atggcatcgc taatgtttat ggttacccct tatgtgtgct caggaatcca
     6541 caggtaatca ctcatactga acagcgataa aagataaaag gtgtgttcat gaattcatgt
     6601 gattttcgtg tttttctgca agagttcggt acaacggttc atttgtcatt gcctggtagc
     6661 gtatccgaga aagaacgact gctactcaag ctgctgatgc agggaatgtc tgtaacagaa
     6721 atatcacagt acagaaatcg cagtgcaaag accatttcac atcaaaagaa acagctcttt
     6781 gagaaactgg ggattcagag cgatattact ttctggcggg atattttctt tcagtacaat
     6841 ccggagatca tatccgccac gggtaataat agtcacaaat atattaatga taatcactat
     6901 caccatatcg tcacgcctga agccatcagt ctggcgttgg aaaaccacga gtttaaaccg
     6961 tggatccaac cggttttctg cgcgcagact ggggtactga caggctgtga agtgcttgtc
     7021 cgctgggaac atccacaaac gggaatgatc ccaccggatc agtttattcc tctggcggag
     7081 tcatccggtc ttattgtcat aatgactcgc cagctgatga aacagactgc ggatattctg
     7141 atgccggtaa aacatttgct gccggacaat ttccatattg gtatcaacgt ctcggcgggc
     7201 tgttttttgg ctgcgggatt tgaaaaagag tgtctgaacc tggttaagaa attaggtaac
     7261 gataaaatca agctggttct tgagctgacg gaacgtaatc ctattccggt aacgccagaa
     7321 gccagagcga tatttgacag ccttcatcag cacaatatta cttttgcgct ggatgacttt
     7381 ggtacggggt atgcgaccta tcgttacttg caggcgttcc cggtcgattt tattaaaatt
     7441 gacaagtcgt ttgtgcaaat ggcgagtgtc gatgaaatct ccggtcatat tgtggacaat
     7501 attgtcgaac tggcgcgtaa gcctggtctg agtatcgtgg cggaaggggt agaaacccag
     7561 gagcaggcgg atttaatgat cggtaaaggc gttcactttt tacagggcta tttgtactct
     7621 ccgccagtac cgggtgataa atttatctct gaatgggtaa tgaaaacagg tggttgatgt
     7681 aaaccgctat tcacagcgca tcgggaggtt ggcagcgatt aattctcccg atgcagtaaa
     7741 tcctgataga tccccgttaa tatgccattt ggcgcaaatt gttttttaat ccacggcgtc
     7801 acctgaccgg tggcagagtg ctgggttgcg agtaacatgc gtgaatcctg gcgcgggtta
     7861 tggatttgtc gggtaaccag caaggattgc gccatcgcct cacggaccat gtaatccggc
     7921 aaaaaaccaa ttccttcacc taaaatctgg cactgacatt tggtgttgaa atctggcacc
     7981 agaattgact cctgcccatg cagcaaccag cccacttttt tattaatcgt atgcgcggtg
     8041 tcctccacca tgatattagg gtagagacgc agctggcttt cggcgatcgg ctccggcaca
     8101 aaggctaatg ggtgatccgg ggcgatggca aaagcccagc gaatcgcgcc aatctccgtg
     8161 taatcaatgc cgccaccgtc cagcagcgtg tcgggggcgc cgatagcgat atttgcctga
     8221 ttattaataa tcgcatccca gacgccgtta tacacttcgg tggtgacggt aatctggcag
     8281 gtaggaaact gctttttcag cacctgtaat aagcgtgcgg tgtgtttggg cgtataaagc
     8341 agctgattaa tacaaatgcg cacacgcgct tctatgccct gcgaaatagt atcgatactg
     8401 cgtttaatgg catgaaaatc attcagcagg tcggtagctt tgcggaaaaa ataacgcccg
     8461 gactccgtta actcaatgct gcgggtactg cgagtgaaca gcaccacatc cagccccgtc
     8521 tccatacgtt taatggtgta gctaatggcg gaagtggtta aacccaactc ttctgcggct
     8581 ttactgaagc tgccaaaacg cgcagcggta gtaaatgcca acagattctc ttcggtaaaa
     8641 attgagttca ttgacccgct cccgccattt tcgattctga atttctccag ccatgactgt
     8701 agaggtatcc attaaagatg gataatcatt tttgaataac ttttaatacc cgtcgctttt
     8761 ggtcgtcgct tcgcttaatt tttgtccgcc gtattctgaa attatcccca cgcagttacc
     8821 cgccagaaaa cccagtaatg tcaccgtcca gttcattccg ctggcaaaaa atagcgtcat
     8881 gccgagaaag cccccgggaa taaaagacag taaccagaaa cgtccttgcc agaccactac
     8941 cgccgagaag ggtaca
//
LOCUS       DAWDRC010000087         8848 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3914_length_8848_cov_2.571477, whole genome shotgun sequence.
ACCESSION   DAWDRC010000087 DAWDRC010000000
VERSION     DAWDRC010000087.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8848)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3914_length_8848_cov_2.571477"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    838..847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2020..2029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2133 a         2350 c         2341 g         2004 t
ORIGIN      
        1 cgccccacct atcgccaata gcataatcgc caacgtatag cggaacagcg gggagaaatc
       61 gccctgatga atttcggtca gcggcacgcg cacccagata ttgggcgaca gccaggtttt
      121 cagccagacg acaggcgagc ttttgttgac ctcaacgcga acttccgtcg gaccgcccag
      181 ttgctgcgcc atctgatggc ttaagaattc atagtgttgc gcccaacgca gacctgcctc
      241 ttcggcagct tcgttggaat agagagagat ccccagctca cggtagatct cccgacggaa
      301 agcgggaggc acaaccaact gcgtgccgtc ctccagttgc agtttgtcgg tcatcaacat
      361 acgcacttcg tacgcgagga ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt
      421 cagcaccacc agataagtcg tcaccaggct gacgaacagc aaggtgacga tgagcaataa
      481 cgtacgggca aatgaacttc gtggcgagaa gcgcaatcgc ctcatgcttt agaaccgtcc
      541 ggtacaaaga cgtagcccag accccagacg gtctgaatgt aacgcggatg cgccggatct
      601 tcttccacca tgcggcgcag acgcgaaatc tgcacgtcga tggagcgttc cattgcggaa
      661 tattcacgac cacgggcaag gttcatcagc ttatcgcggg agagcggctc acgcggatgg
      721 ctgaccagcg ccttaagtac cgcaaactca ccactggtga gcggcatcgg ctcgtcttcg
      781 cggaacattt cacgcgtacc gaggttaagt ttgaacttac cgaaagcaat taccgccnnn
      841 nnnnnnnctc ttcctgtgac ggcgcgcccg gcagttcgtt ggcctgacga cgcagcaccg
      901 cacgaatgcg ggccagcaat tcacgcgggt taaacggttt tggaatgtag tcgtcagcgc
      961 caatctccag gccaacgata cggtccactt cttccccttt cgccgtcacc ataatgatcg
     1021 gcatcgggtt gctctggcta cgaagacgtc ggcaaatcga caagccatct tcaccaggta
     1081 acatcaaatc cagtaccata agatggaaag attcacgagt cagcaggcga tccatctgtt
     1141 ctgcattagc gacgcttcga acctggaagc cttgttcggt gagataacgt tccagcagcg
     1201 cgcgcaggcg catatcgtca tcgaccacca gaatcttgta gttctcttgc attgtttgta
     1261 ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta aaaaagctca cgttcgtcac
     1321 cagctaaatc tggtatgaat ttcagcctaa attgttacaa agcatattaa acagcagctt
     1381 aagtatacaa tttattcggc gaaacattat tgattctgtt gatatgatca cgttataccc
     1441 aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga tgaaaacgcc cctggttacc
     1501 cgggaagggt atgaaaaact caaacaagag cttaattatc tctggcgtga agaacgcccg
     1561 gaggtcacaa aaaaggtgac ctgggccgca agtctgggcg accgcagcga aaatgctgac
     1621 tatcagtata ataaaaagcg cctgcgtgaa atcgaccgtc gcgtgcgcta tctcactaaa
     1681 tgcatggaaa atctcaaaat cgtcgattac tccccgcagc aggaaggcaa agtctttttt
     1741 ggtgcgtggg tggagattga aaacgacgat ggtgtgactc accgtttccg tattgtcggc
     1801 tacgatgaaa tttttggccg taaagattac atctctatcg attccccgat ggcccgcgca
     1861 ttgctgaaaa aagaagtcgg cgatctggcg gtggtgaata cccctaccgg ggaagcgagc
     1921 tggtatgtta atgctatcga gtacgtgaaa ccgtaaggaa gagtcttaac ctcatgccga
     1981 tggctggcat ttttgccagc cagtccgtat aactatcccn nnnnnnnnng tgccgtttat
     2041 cgcacgttat cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga
     2101 aacgcgtctg agctatctgc gcgagctgga agagagacgt caggcgatcc tcaagtctat
     2161 ttccgagcaa ggcaaactca ccgatgatct ggcgaatgcc atcaacgcca ccctaagcaa
     2221 aaccgaactc gaagacctct acctgcccta caaacctaaa cgccgcaccc gtgggcaaat
     2281 cgccattgaa gcagggcttg agccgttggc cgatctgctg tggagcgatc cgtcacacac
     2341 gccagaagtc gccgctgcac aatatattga tgccgataaa ggcgtggcag ataccaaagc
     2401 cgcgctggac ggcgcgcgct atatcctgat ggaacgtttt gccgaagatg ctgcgctgct
     2461 ggcgaaagtg cgtgattatc tgtggaagaa cgcgcatctg gtctcaaccg tggtgagcgg
     2521 taaagaagag gaaggggcaa aattccgcga ctatttcgat catcacgaac cactgtcaac
     2581 ggtgccttct caccgcgcac tggcgatgtt ccgtggtcgt aacgaaggca tacttcagct
     2641 ttcgctgaat gccgatccgc agttcgaaga gccgccgaaa gagagctatt gcgagcaaat
     2701 catcatggat caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaagg
     2761 tgtggtgagc tggacgtggc gcatcaaggt gttgatgcat ctggaaactg aactgatggg
     2821 caccgtgcgc gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga
     2881 tctgctgatg gcggcccctg ccgggctgcg tgcaacgatg ggcctcgatc cgggtctgcg
     2941 taccggggta aaagtggcgg tagtcgatgc gacaggtaaa ctggtggcga ccgataccat
     3001 ttatccgcat accgggcagg ccgcaaaagc agcgatgacc gttgctgcgc tgtgtgaaaa
     3061 gcataacgtt gaactggtgg cgattggcaa cggtacagct tcccgcgaaa ccgaacgttt
     3121 ctatctcgac gtgcagaagc agttcccgaa agtgaccgcg cagaaagtga tcgtcagcga
     3181 agctggcgcg tcggtttact cggcttccga gctggcagca caggaattcc cggatctcga
     3241 cgtttcgctg cgtggcgcgg tgtctatcgc ccgtcgtttg caggatccgc tggcggagct
     3301 ggtgaaaatc gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac
     3361 gcagctggca cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga
     3421 tctcaacacc gcttctgttc ctctattaac ccgcgtggcg ggcctgacgc gcatgatggc
     3481 gcaaaacatc gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgtt
     3541 aaaagtgagc cgtctggggc cgaaagcctt cgagcagtgc gcgggcttct tgcgcattaa
     3601 ccacggtgat aacccgctgg acgcttctac cgttcacccg gaagcctatc cagtggtgga
     3661 acgcattctg gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact
     3721 gcgtaacctg aaagcatctg actttactga tgagaaattc ggtgtgccga cggtaaccga
     3781 catcatcaaa gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca
     3841 gtttgccgat ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggtgc
     3901 ggtgaccaac gtcaccaact ttggcgcgtt tgtcgatatt ggtgtgcatc aggacggcct
     3961 ggttcacatc tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc
     4021 gggcgacatt gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct
     4081 gaccatgcgt ctggacgagc agcctggcga aaccaacgcc cgtcgcggtg gcggtaacga
     4141 tcgcccgcaa aacaaccgcc cggcagccaa accgcgtggg cgtgaagcgc agcctgccgg
     4201 taatagcgcg atgatggatg cgctggcggc ggcaatgggc aaaaaacgtt aaacgcccgg
     4261 actggcccct aaacctgcgc gattaggcgt tcacgccgca tctggcaatg aacgccttgc
     4321 ctgacataaa agtgccggag aatatctccg gcatttttat tccacagcca accccacaat
     4381 atactcccta atatttatca cctcattaac aactgaaacc ttaattaaac attaaccagc
     4441 cctaataatt cgtcatgcga attatatttt cgctgcgata taaccttgag ccatatcaac
     4501 attgagtcag attattattc aaaccaacat tcacacacat tttaagtatt gctgatagaa
     4561 accattctca ttatcattgt actgttgatt atttaatctc tccttcgttg gcaaatcatc
     4621 tggtctcatg tcgctgtcaa acgccccatg aggtagttat ccagttaatg agaaacaagt
     4681 aggcacctat gcaatacact ccagatactg cgtggaaaat tactggcttt tcccgtgaaa
     4741 tcagcccggc atatcgccaa aaactgcttt ctcttggcat gttacctggc tcctctttta
     4801 atgtggtgcg cgtcgctcca ctcggcgacc ccattcatat cgaaacccgt cgtgtgagcc
     4861 tggtattacg caaaaaagat ctggccttat tagaagtgga agcggtttcc tgttaatacg
     4921 gtgataacaa caatgaaaaa attaaccatt ggcttaattg gtaatccaaa ttctggcaag
     4981 acaacgttat ttaaccagct cactggctca cgtcagcgtg taggtaactg ggctggcgtt
     5041 accgtcgaac gtaaagaagg gcaattctcc accaccgatc atcaggtcac gctggtggac
     5101 ctgcccggca cctattctct gaccaccatc tcatcgcaga cctcgctcga tgagcaaatc
     5161 gcctgtcact acattttgag tggcgacgca gacctgctga ttaacgtggt ggatgcgtct
     5221 aaccttgagc gtaacctgta cctgacgctg caactgttgg aactcggcat tccatgcatc
     5281 gtggcgctga acatgctcga cattgccgag aagcaaaata ttcgtattga aattgatgcg
     5341 ctgtcggcgc gtcttggctg cccggtgatc ccgctggttt caacccgtgg tcgcggtatt
     5401 gaagcgctca agctggcgat tgatcgctat aaagctaacg aaaatgtgga actggtgcat
     5461 tacgctcagc cgctgctcaa cgaagcagat tcactggcaa aagtgatgcc ttccgacatc
     5521 ccgctgaaac aacgtcgctg gctgggcctg caaatgctgg aaggcgatat ctacagccgc
     5581 gcctacgccg gtgaagcgtc gcagcatctg gatgccgccc tcgcccgtct gcgtaatgag
     5641 atggacgatc cggcgctgca cattgccgat gcgcgttacc agtgcattgc tgccatctgt
     5701 gatgtggtaa gcaacaccct gacggcagaa cccagccgtt tcaccactgc ggtagataaa
     5761 atcgtgctca accgtttcct cggtctgccg attttcctct ttgtgatgta cctgatgttc
     5821 ttgctggcta tcaacatcgg cggggcgtta cagccgctgt ttgacgtcgg ctccgtagca
     5881 ctctttgtgc atggtattca gtggattggc tacacgctcc acttcccgga ctggctgact
     5941 atcttcctcg cccaaggcct gggcggtggt attaacaccg tgctgccact ggtgccgcag
     6001 atcggcatga tgtacctgtt cctctccttc cttgaggact ccggctatat ggcgcgtgcg
     6061 gcgtttgtga tggaccgtct gatgcaggcg ctgggcttgc cggggaaatc ctttgtaccg
     6121 ctgatcgttg gtttcggttg taacgtaccg tcggtaatgg gcgcacgtac gcttgatgca
     6181 ccgcgtgaac gtctgatgac catcatgatg gcaccgttta tgtcctgcgg cgcgcgtctg
     6241 gctatcttcg cagtattcgc ggctgccttc ttcgggcaga acggtgcgct ggcggtcttc
     6301 tcgctgtata tgctgggtat cgtaatggcg gtgctgactg gcctgatgct caagtacacc
     6361 atcatgcgcg gtgaagcgac gccgtttgtc atggagctgc cggtctacca tgtaccacac
     6421 gttaaaagcc tgattatcca gacctggcag cgtctgaaag gcttcgttct gcgtgctggt
     6481 aaagtgatta tcatcgtcag cattttcctg agcgccttca acagcttctc gctgagcggg
     6541 aaaatcgtcg ataacatcaa cgactcggcg ctggcttccg tcagccgggt gatcaccccg
     6601 gtcttcaagc cgattggcgt acatgaagat aactggcagg caacggttgg cctgtttaca
     6661 ggtgccatgg cgaaagaagt ggtggtgggt acgctcaaca ccctctacac cgcagaaaat
     6721 attcaggacg aagagttcaa tccggcggaa tttaacctcg gtgaagagct gttcagtgcg
     6781 gtagatgaaa cctggcagag cctgaaagac accttcagcc ttagcgtact gatgaacccc
     6841 attgaagcca gcaaaggcga cggcgaaatg ggtaccgggg cgatgggcgt gatggatcag
     6901 aaattcggta gcgcagctgc cgcatacagc tacctgattt tcgtcctgct gtacgtaccg
     6961 tgcatctcgg tgatgggggc tatcgcgcgt gaatcaagcc gtggctggat gggcttctcc
     7021 atcctgtggg ggctgaatat tgcttactca ctggcaacat tgttctatca ggtcgccagc
     7081 tacagccagc atccaactta cagcctggtg tgcattctgg cagttatcct gtttaacatc
     7141 gtggttatcg gtctgctgcg ccgcgcgcgt agccgggtag atgtcgaact gctggcaacg
     7201 cgcaaatcgg taagcagttg ctgtgcggcc agcaccaccg gtgattgcca ttaatggctt
     7261 cacttattca ggtgcgtgat ttgctggcgt tacggggccg tatggaagcg gcccagataa
     7321 gccagacatt gaacactccg cagccaatga ttaacgccat gctgaaacag ctagaaagta
     7381 tgggaaaagc cgtgcggatt caggaagaac ctgacggctg tctttccggc agttgtaaaa
     7441 gctgcccgga aggaaaagcc tgtctgcacg aatggtgggc gttgcgttaa ccttgtttca
     7501 ctgcctgttt tgaaagcccg gtatgcgttt gcatccgggc ttttttgcgt gcggctttcc
     7561 ataaaaatgc aacccttgca gcgcgacgta agttcctctg aaagcgtctc gcagggatga
     7621 aaactcgcta atgcacaggt gtggagtggc gcgtagagtc gcggcattca aataacaggt
     7681 gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgtta cgccattgcc
     7741 gcaatgcaaa atcaccttga tgccggatac aaaacattgc cgatggtggt gccattgttg
     7801 ttttatcacg gtattgaaag cccctatccc tattcgctgt gttggctgga ttgtttcgcc
     7861 gatcccaacc tggcaaggca gctttatgcc tctgcatttc cgctgattga tgtcaccctc
     7921 atgcctgatg atgaaatcat gctgcaccga cgtatgccgc tactggagtt aattcaaaaa
     7981 catattcgtc aacgcgacct gatggggctg gtagagcaaa tggcctgctt attaagtagt
     8041 ggatacgcta atggcagaca aatcaaaggg ctgtttaatt acatactgca aaccggcgac
     8101 gcggtacgtt ttaacgattt tatcgatggt gtggccgaac gttcaccaaa acacaaggag
     8161 agtttaatga ctattgcgga aagattgcgg caggaagggg aacaatccaa agccttgcat
     8221 atagcaaaaa taatgcttga atccggagtc cctcttgcag acatcatgcg ctttaccgag
     8281 gtgtcagaag aagagttggc tgcggcgagc cagtaaagtt ctgtcccgcc atttcgaaag
     8341 ccacctacac cctctgcttc aacgccacca gcaggtgaca aaactcgtcc ggatgtgaaa
     8401 taaacggcgc atgggccgct ttggcgaaga tatatgattc gctgtgaggc caaagtttat
     8461 ccagcatcgg caccactttg cgcggcacca gaccgtcgag atagccatac aatcgcaaaa
     8521 acggcatggg cacgttttgc agcggctgac ggagatcgac cgttttcagg atttccagcc
     8581 cgccattaag cacgtcaacc tccggcatcg gtaacgccag aacggttttc ttcaacgccc
     8641 gcgcatcctg gcgcgccgtt tcagtaccca tggtttgtaa cgccaggaac cgctccactg
     8701 tacgctgaaa atcatcactc agctgctgct gaaatcccgc cagcacgtcc ggttttatcc
     8761 ccggccactc gtcgcgagca ctaaaacaag gtgacgacgc cacggtgacc agcgcctgaa
     8821 cccgctcggg atgggttaac gcaatctg
//
LOCUS       DAWDRC010000088         8831 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3921_length_8831_cov_2.525752, whole genome shotgun sequence.
ACCESSION   DAWDRC010000088 DAWDRC010000000
VERSION     DAWDRC010000088.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3921_length_8831_cov_2.525752"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4259..4268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1864 a         2325 c         2461 g         2171 t
ORIGIN      
        1 cgggtggtca tacgctgggt aaaacccacg gtgccggtcc gacatcaaac gtaggtcctg
       61 atccagaagc agcaccgatt gaagaacaag gtttaggctg ggcgagcact tacggcagcg
      121 gcgttggcgc agatgccatt acctctggtc tggaagtggt ctggacccag acgccgaccc
      181 agtggagcaa ctatttcttc gagaacctgt tcaagtatga gtgggtacag acccgcagcc
      241 cggctggtgc aatccagttt gaagcggtag acgcaccgga aattatcccg gatccgtttg
      301 atccgtcgaa gaaacgtaaa ccgacgatgc tggtgaccga cctgacgctg cgttttgatc
      361 ctgagttcga gaagatctct cgtcgtttcc tcaacgatcc gcaggcgttc aacgaagcct
      421 ttgctcgtgc ctggttcaaa ctgacgcaca gggatatggg gccgaaatct cgttacatcg
      481 ggccggaagt accgaaagaa gatctgatct ggcaagatcc gctgccgcag ccgatctaca
      541 acccgaccga gcaggacatt atcgatctga aattcgcgat tgcggattct ggtctgtctg
      601 ttagtgagct ggtatcggtg gcctgggcgt ctgcttctac cttccgtggt ggcgacaaac
      661 gtggtggtgc caacggtgcg cgtctggcat taatgccgca gcgtgactgg gatgtgaacg
      721 ccgcagccgt tcgtgctctg ccagttctgg agaaaattca gaaagagtct ggcaaagctt
      781 cgctggcgga tatcatcgtg ctggcaggtg tggttggcgt tgagaaagcc gcaagcgccg
      841 caggtttgag cattcatgta ccgtttgcgc cgggtcgcgt tgatgcgcgt caggatcaga
      901 ctgacattga gatgttcgaa ctgctggagc caattgctga cggtttccgt aactatcgcg
      961 ctcgtctgga cgtttccacc accgagtcac tgttgattga taaagcccag caactgacgc
     1021 tgaccgcgcc ggaaatgact gcgctggtgg gcgggatgcg cgtactgggt gccaacttcg
     1081 atggcagcaa aaacggcgtc ttcactgacc gcgttggcgt attgagcaat gacttcttcg
     1141 tgaacttgct ggatatgcgt tatgagtgga aagcgaccga cgaatcgaaa gagctgttcg
     1201 aaggccgtga ccgcgaaact ggcgaagtga aatacacagc cagccgtgcg gatctggtct
     1261 ttggttctaa ctctgtcctg cgtgcggtgg cggaagttta cgccagcagc gatgcccacg
     1321 agaagtttgt taaagacttc gtggcagcgt gggtgaaagt gatgaacctc gaccgtttcg
     1381 acctgctgta atctgacctt cttcagcgac tgcctttcat gcagtcgctg aagtttcttt
     1441 accggcgtat agtgtccaca ggaaaactac acactggatc tctcatgtct gccgcaggaa
     1501 agagcaaccc actggcaatc agtggcctgg ttgtgctcac acttatctgg agttatagct
     1561 ggattttcat gaagcaagtc accagctaca tcggtgcctt cgacttcacc gccttacgct
     1621 gcattttcgg cgctctcgtt ttattcatcg tccttttatt acgtggtcgc ggaatgcgcc
     1681 cgacgccgtt taaatacacc ttagccattg ccctgttaca aacctgcggg atggttggcc
     1741 tggcacagtg ggcgctggtc agcggcggag cggggaaggt ggcaatcctg agctatacca
     1801 tgccgttctg ggtggtaatt ttcgccgcgt tgtttctcgg tgaacgcctg cgacgtgggc
     1861 aatatttcgc gatactgatt gccgctttcg gcttattttt ggtgttgcag ccgtggcaac
     1921 tcgatttctc ttcgatgaaa agtgccatgc tggcaatcct ctccggtgtg agttgggggg
     1981 cgagcgcgat tgttgctaaa cgtctgtatg cccgccatcc gcgcgtggat ttattgtcgc
     2041 taacatcctg gcagatgctg tatgcggcgc tggtgatgag cgtggtcgct ttactggtgc
     2101 cgcaacgtga aattgactgg caacccaccg tgttctgggc gctggcctac agtgcgattc
     2161 tggcgacagc gctggcctgg agcttatggt tgtttgtatt gaaaaacttg cctgccagta
     2221 ttgccagctt aagcacactg gccgttcccg tttgcggcgt actcttttcc tggtggctgc
     2281 tcggtgagaa tccgggggca gttgaaggtg gcggtattgt gctgattgta ctggcactgg
     2341 cactggcgct ggtgagccgt aagaaaaaag aagccgtcag tgtaaaaagg atctgaattt
     2401 tttcttcatg tggggagatc tcttatttag caaaataccg atagtgcccc accatctgcc
     2461 agctaaacag tacatcttct tcctgcgcgc ctgcgccaat gttatgtatc accagcggcg
     2521 taccgtcgcg ggcgaagcca tctgacacca ccccaatatg tgccagcccg ttatctaatc
     2581 gccaggagac aatatcgccc gcttgatagt cactggggtt cttgctggtg gggcgtgttt
     2641 tatcgtggcg ggtaaaccag gtttccagat taggcacccg acggtgatcg atgttgctgt
     2701 ccgggcgctt taactgccat ttttgcgggt acgcagcaaa attcttcgcc atatcttcgt
     2761 gcaccagttt ctgcaaatcg accttctggc tgcgcaatgc acggatcacc acatcggaac
     2821 atacgccgcg ttcttgcggg acatcaccgc caggataggt aagctgcaca tacgccggat
     2881 cgtaaaatag cgtgctgccg atttgcagtc tggcgccgtc tgcgatggca agattggtgt
     2941 tagcctggat ttgtaccacg gttggtggaa cggcgggaga ttttaaggag taactggtaa
     3001 ataacgtcag aagactgaat aacgccagtg aagctttcat ctgtttcgtc cgtgaaaata
     3061 agagtcacag attcgacctt ccgggctaac gattgttctg tggagaaaat gtccagaagc
     3121 ggataaaaaa acacccgaac aaggtggaag tttcagatta ctggcacagc gtgccggatg
     3181 cggcgtgaac gccttatccg gcctacaaaa gtacgtaaat ttactatatt gcagagattg
     3241 cgtaggcctg ataagcgtag cgcatcaggc aattttgcgt tcaaactccc ggacaagccg
     3301 ggagtttgga gtaggttatt cccactcttg caggaaacgc tgaccatact ggtcggctac
     3361 cagcagtgcg gcgtaaacct gatctggcgt cgcgccgcca ggcatgttgt ggatggtttc
     3421 accttctgca catgccgctt ctgccacaat tcgcattttc gccgggacat cttctctaat
     3481 atccagttga gcgagagtta ttggcaaacc taccgcatgg ctaagcgcag ctacggtttc
     3541 gatttcctcc accggcgcgt tttccagcac cagctgcgtc agcgtaccga atgccacttt
     3601 ttcaccatga taatagtgat gcgcgtccgg gatagcggtc aggccgttat gtaccgcgtg
     3661 tgccgccgcc agcccgccgc tttcaaaacc aacgccactc aaataggtgt tcgcttcaat
     3721 cacgcgctcc agcgctggag tcactacatg ctgttcggcg gcaagcatcg ctttttcgcc
     3781 ttcttccagc agggtgttgt agcacagttc agccagtgcc agcgctgcct gagtgcactt
     3841 gccacccgcc atggtggtcg cgccgctacg ggaacaggca cgcgcttcaa accaggttgc
     3901 cagcgcatcg ccaatacccg ccgctaacag acgcgcgggc gcgccagcga cgattttggt
     3961 gtcgacaatg accatattcg ggttatttgg caacagcaga tagcggtcaa actcaccctc
     4021 atcggtgtag ataacagaca atgcgctgca cggtgcgtcg gtagaagcga tagtcggtgc
     4081 gatcgctacc ggaacaccca tgaaatgtgc cagtgctttg gcagtatcga gggtttttcc
     4141 gccaccgata ccgagaattg cgccacactg cgcagtctcc gcgatgccac gcagacggtc
     4201 gatctcattt tgcgaacatt caccgccaaa cggcgcaatt tctactacca gtccagcann
     4261 nnnnnnnnga ttgagcaaaa cctaaaacaa atttgtcacc caccactaac cagcgttccg
     4321 ccagtggctt caggtactcg cccagacgat taattacatc tgcgccctgg atgtatttac
     4381 ctggtgattg aataatgcgg tccataatta ctcctttaga gatgagtagt gtcaaatgcg
     4441 gcattccagt cgtgctcgaa cttctctata gctgactcta ccgcaggggt gttaagcatt
     4501 tgttgcgcta catctaaggg cagtgtgatg gattcacatc cagccagcaa acaatccagc
     4561 gcctgacgtg gtgttttaaa gctggcagcc agcaccatgc tttctggcgc atgcatttcc
     4621 agtaacgctt gcaactcctg tacagtacga atgccgtcac cgccctgggc atctacgcgg
     4681 ttaacgtatg gagcaacgta tttggctcca gccagcgccg ccagtaatcc ttgcgcggcg
     4741 ctgtacactg cggttcccag cgtagtaatg ccttctttct tcagcatttt aattgctgcc
     4801 agaccttcag aggttaccgg aattttcacc acaatgcccg gaatagcgtc gcgcaggtgt
     4861 ttcgcttctt tcaccatacc ctgcgcgtcg cggctcatgg tctgagcaaa cagaatgccc
     4921 tcatcaccga tcgctttttg aaggcgcggc agcacttccc agatggactc cttgctggca
     4981 gcgataatgc tcgggttagt tgtcaccccg gcaatcggga atatgcgtgc cagacgttcg
     5041 acttctgcga cgttagcggt gtccagatac agttccatga tgtttccttt tacagttcca
     5101 gttcatgttg cagcaggctg gcgatagcgt cggcagaggc ggcgttaacc agcgcattac
     5161 ggaattcttc gtgcatgatg cgacgagcga ggcgcgagaa aatgcgcata tgttgatcgc
     5221 ccgcggcgtg tttgttcagg gttaacataa tgatgaattg cgcttcatca tcgccccagc
     5281 gcactggcgc ttgcagacgc gctacgctga tggtggattg ctcaatgtgt tccgatttac
     5341 tgtggggaat ggcgaaacta aagcccagac cggtagagaa aacggcctcg cgcgcccaca
     5401 agtcggcttc cagtttacgc ggatagcggc agcggcccgc cagcagcagg ttatcggtca
     5461 tgcctttgag cacttcttct ttgctgcgcc agtcgctttc cagtgtgatg cactcggcgg
     5521 tgaccagcgg tgcgtcctgt tgggtcatgc ggaattgcgc cagcaggtgt tccacttcca
     5581 gcgaagtacg gcaggccatt gcctggttga gcaactggcg gcactcacgg ctatcaagtt
     5641 gcgccatccg cgctttcgcc gccggaattg atggtgcgct catgctgagt tcatccagcc
     5701 ctaaaccgac cagcaacggc agcacggaac ctttcgctcc cagttcaccg cacagaccaa
     5761 tccatttgcc ctggcgatgc accgcctgca cggcgtaatc gagcgcccgc aagaaggccg
     5821 gattcaggct gttgtagtga cgagtcacct tagcgttatc gcgatccact gccagtagat
     5881 attgcgtcag gtcattacta ccaatactaa agaaatcaat ctcttcgcag cattgatcga
     5941 tgatgaacat caccgacggc acttccagca tgatgccgag ctggatcttc tcatcaaacg
     6001 gaatgtgttc gttacgcagt tgctgtttgg cttccgccag tttttctttc acccataaga
     6061 tctcttccat tgaggagatc atcgggatca tgattttcag gctgccgtga gcagaggcgc
     6121 gcaggatcga ccgtagttgt gtggtaaaca acgacgcgta ttcttcataa atacgcaccg
     6181 cgcgataacc gaggaacggg ttagcttctg ccggaatgtt cagataatca acgggtttat
     6241 caccgccaat gtccatagtg cgcacaataa tgctgcgtcc gttggcagac tccagcgcct
     6301 gacaaaaaat gttgtacagc tcgctttcgc ccggtgcact ggtgcgatcc atatagagca
     6361 tttcagtgcg gaacaaacca acgccttccg caccattgcc gaatgcggcc tgcgcttcca
     6421 cggagtgagc gatgttagcg gcaatttcaa tgcggatacc gtcagcggta cgcgcttgct
     6481 gggtcagcca gacacgctgt tgctcacgca gagcgtcctg tacgcgggct tcttgctgat
     6541 aataacgggc cacggattcc cctggctcaa ccacaatcgc ccctgcgttg ccgtcgatat
     6601 aaatcgtctg atgctgccac ggagtaaggg cagcaatatc cacacctacc agcgtcggaa
     6661 tgttgaacga acgggcaagg atcaccgtat gtgatgtggt gccgccactt ttcagtaaca
     6721 gacctttgag atgattttta tccagttcga ggaactggct gggggtcagt tcatcagcca
     6781 tacaaatggc gggctgcgtc agtttgcccg gtgccgggaa gcgttgctca ccgtagattt
     6841 gctggagtaa ctggaagcag acgtcgcgta cgtccagggc acgttcttgc aggtagctgc
     6901 tgctggaacg ggcgaactct tcgcaaaagt gattcgcact ggcaacaatc gcttcggcgc
     6961 agcttaatcc ggcgctgaca cctgccagta aatgttcgcg cagggaagta tcgccagcca
     7021 gggatcggtg cgcttccaga atcgcgctgg tagcaccgtc gctatccagc agacgaaact
     7081 ctatgttttt cagtaccagc gtcagaccgt tttccagtgc ggattgttcg gcgtcaacgc
     7141 ctttggctgc gggaagatta cccagcgcat tgagatctaa agaagagatc ggcgtcagaa
     7201 tgccgcctgc gctaccactg cacacggtgc gagcacggat aatttgcgga ttcagattgg
     7261 tcagtgaaac cggcagtggt tccagttcgt cagattttac ttccgccagc ggtgcgtcgc
     7321 agtgggggaa ttcatcgcgc agccattggc ttaaacgctg gtacgcttcc tgttcgtcgg
     7381 ccccggaaat cagtaactgg cagttatcgc ccaccagcgt atcggtgcca atcagcgcaa
     7441 gggcgctttt ggcgttgccc ttgcggtcag tgcgcaggtt atgccactca atttgtgatg
     7501 aaaaagtatt acacagcgtt tcaacgtggc ttgccggacg cgcatggacg ccgttaggta
     7561 gctcacaaat aaattccaca atcagggcca ttgcctttct cccggtacgg ttttctgttt
     7621 acaggataag agcgcacggt aacggcctgc catgtgacaa atctgccaaa agctggacaa
     7681 atgtaatgta accgtcaatt tgcgacgcgt ctcacaagac gccgttttgc ggcatgcttc
     7741 cggattatcg caagttatga ggcggatcgc atttttgtac tgatattaca aaagtccagt
     7801 aaatggcctt tttatccact gtttgctccg cctgcgattg cctattgttt cttccatcaa
     7861 aaatatggac atgggctttc gcaagcccgg gagcagctta tgaatgagtt ggtgcagatc
     7921 ctgaaaaata cccgtcagca tttaatgacg ggcgtttcac acatgattcc cttcgtggta
     7981 tcgggcggta ttttgctggc ggtttccgtc atgttgtatg gcaaaggcgc agtgccggat
     8041 gccgtagccg atccgaatct gaaaaaactg tttgatatcg gcgttgcagg tttgacgctg
     8101 atggtgcctt tcctcgccgc ttacatcggt tattccattg cagaacgttc tgcgctggct
     8161 ccatgcgcta tcggtgcctg ggttggtaac agctttggcg cgggcttctt tggtgcgctg
     8221 atcgccggga ttatcggcgg catcgtggtg cattacctga agaaaattcc ggtgcataaa
     8281 gttctgcgct cggtgatgcc aatcttcatc attccaatcg tcggcacact gattaccgct
     8341 ggcgtcatga tgtgggggct gggcgagccg gtgggggcgt tgaccaacag cctgacccag
     8401 tggcttcagg ggatgcagca gggcagcatt gttatgctgg cggtgatcat gggtctgatg
     8461 ctggcgttcg atatgggcgg cccggttaac aaagtggcct atgccttcat gctgatttgc
     8521 gttgctcagg gtgtttatac cgtggtggct attgccgctg ttgggatttg tgttccaccg
     8581 ctggggatgg ggctggcgac gctgattggt cgtaaaaatt tctccgcaga agagtgcgaa
     8641 accggcaagg cggcactggt gatgggctgt gttggcgtta ctgaaggggc gattcctttc
     8701 gccgctgccg atccgctgcg tgtcattcct tccatcatgg tcggttcagt ttgtggtgcg
     8761 gtaactgcgg cgctggtcgg tgcgcagtgc tatgcaggct ggggtggtct gattgtgctg
     8821 ccggtggttg a
//
LOCUS       DAWDRC010000089         8707 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3973_length_8707_cov_2.014216, whole genome shotgun sequence.
ACCESSION   DAWDRC010000089 DAWDRC010000000
VERSION     DAWDRC010000089.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3973_length_8707_cov_2.014216"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    239..248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1258..1267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5204..5213
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6763..6772
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2009 a         2190 c         2390 g         2078 t
ORIGIN      
        1 cggcagaaag attttcggcg gcagctggct ggcaccaatc agcgtcgggc caatgacccg
       61 aaagccatac ataaaacgcg taccaatgac aaacagatac ggatggcgtt ggataagttt
      121 ctgcgcccgc tcaattttat cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa
      181 ccgccgcccg cacagataaa gcacctggtc gccaatcatg ccgccaagcg ccacagaann
      241 nnnnnnnncc agcgggaact ttaatagccc ctgatgtgcc gcaacgcctc ccagcaaagt
      301 cacggtttca ccttccgcca ggctaccgat caccagcgcg gcataaccat attgtgagat
      361 gagtgtattg agatccatag gacagtctgt ctccctttgc ttgcctgtaa gcatacaccc
      421 tgtgaatcat tcacgctgaa aaaggacgta catcagacgg tgcaaattac atgagcgaac
      481 aaaaaataat cacaagctga cttatacttg aactgtcagg ttcatgaact cacaacaagg
      541 gggcggtatg agccatgtct ggggactgtt ttcccatccc gatcgagaaa tgcaggtgat
      601 taatcgcgaa aacgaaacga tttctcacca ttacacccac cacgttttgc tgatggcggc
      661 gattccggtg atttgcgcct tcattggcac tacacagatt ggctggaatt ttggcgatgg
      721 cactatcctg aagttatcct ggtttaccgg actggcgctg gctgtcttgt tttacggcgt
      781 tatgctggct ggggttgcgg tgatggggcg ggtcatctgg tggatggcgc gtaattatcc
      841 gcagcgtccg tcacttgcgc actgcatggt cttcgcgggc tacgttgcaa ctccgctgtt
      901 tttaagcggt ctggtggcgc tttacccgct ggtctggttg tgtgcgctgg tcggcacggt
      961 ggcgctgttt tacaccggct acctgctgta tctcggtatt ccgagcttcc tgaatatcaa
     1021 caaagaggaa ggacttagct tctccagttc gacactggcg ataggcgtgc tggtgctgga
     1081 agtgctgctc gcccttaccg ttatactatg gggctacggt tatcgtttgt tctagaaagt
     1141 tcttttgcgc tgcattgctg gcgtaaatgt cagcaatgca gcatttcctc acgattcttc
     1201 tttgacgatc gcactttttg ctcgttatga tggcgtcgct agcctcagta aatccttnnn
     1261 nnnnnnncat catgccgaaa tttcgagttt ctttatttag cctggccctg atgctggctg
     1321 tgccttttgc accgcaggcc gttgcgaaaa cggcagccgc taccaccgcg tcacaaccgg
     1381 aaattgcctc cggtagcgcg atgattgttg atctgaatac caacaaagtg atctattcga
     1441 accacccgga tctggtgcgt ccgattgcgt ctatcagcaa attaatgacc gcgatggttg
     1501 tgctggatgc acgactgccg ctggatgaaa aactaaaagt ggatatcagc cagacgccgg
     1561 agatgaaagg ggtctattcg cgcgtacgac tgaacagcga aatcagccgt aaagatatgc
     1621 tgttgctggc gctgatgtct tcagaaaacc gcgcggcggc aagccttgcg caccattatc
     1681 ccggtggtta caaagccttt attaaggcga tgaatgcgaa agcgaaatcg ctcggaatga
     1741 acaacacgcg ctttgttgaa ccaaccggat tgtcggtgca taacgtttca accgcccgtg
     1801 acttaaccaa actacttatt gccagcaaac aatatccgtt gatcgggcag ttaagtacta
     1861 cccgggaaga tatggcaacc ttctctaacc cgacgtatac gctgccgttc cgcaatacta
     1921 accatctggt gtatcgtgat aactggaata ttcagttaac caaaaccggt tttaccaatg
     1981 cggcgggcca ttgtctggtg atgcgcacgg ttatcaataa taaaccggtg gcgctggtag
     2041 tgatggacgc gtttggcaaa tatacccatt ttgccgatgc cagccgcctg cgtacctgga
     2101 ttgaaaccgg taaagtgatg cctgttccgg cagcagcgtt gagctataaa aaacaaaaag
     2161 ccgcccaaat ggcggcggcg gggcagacgg cacagaacga ttaaatgaat gacggatggc
     2221 gatactctgc catccgttat tattactcca cttcctgcca gtttttccgc ttactggttt
     2281 taccgatccc cggattcata ctgttggtcg gatcgttctc gcgatagaac ttctgcaacg
     2341 tctccggtgc tttatacaaa tgaccgacgt tatgctcggc agggtattgc gcgccacgct
     2401 gctgcagcag ttccagcatc tgttctttca acgcatgcac atccacgcct tttttaacga
     2461 tgtaatcctg atggaagaca tagcacataa aatggccgta atagagcttg tgcaccagtt
     2521 ggttgtcgat ctccggcggt aaatgctcat accactcggt gtcgttacgc cgcagagcga
     2581 tatccagcgc cagaatgtct tcgacttcat cggaatgcac agcctgataa cgaattgctg
     2641 cgcccgcagc ggcgaaacgg tgtaaaaacg ctttgctgcc ttcctccggc gtacagacaa
     2701 agaaatcgcc ctcggcctgt ttgaaataat ctaccagcca cgatttggct tcaccgacgc
     2761 catcgcccgc catttttaac agcagatgat gctcgtattt atcgcgccag tttttcatgc
     2821 gcggcggtaa atggctgggg aacaggtgac cgaatttttg cattgcacgg tcggtaaaat
     2881 gcggacggaa gaatttcact ttctccagca tcgcatcggt gcgtcccttg agattaaaga
     2941 agaacggcat cttgtcggtg cctaacttat caatcatcag gaaggtgtct ttgccgtatt
     3001 tttccgcaat atcgtagata tcccggtgca tatattcccc ggcaaccggc agattttcga
     3061 aattagccag aatatgacgg cggatttcgg tcagcacttc cggctggttg gtgccgatat
     3121 aaaacacctg ctgatttttt tccgcttcga aggtatcaag acgtaccgca aagacggcca
     3181 gcttccctgc gcaaccagaa gattcaaata accgatccgg atcagcgtta taacgtgcgg
     3241 gcgtgtcggc ttcaatatca cgaacgcggt ggacatagtc ataatcgtgg gcgtgacgac
     3301 catcgtggcg cacatcgtca tctttgatgc gatcgtcatc cagcttgcta aggatctgct
     3361 ccggcgtttc gcccagatca atccccagat ggttcaccag cgtcagtttg ccgtcttcat
     3421 ttatacgcgc gaataatgac atttcggtat acgccgggcc acgttgcacc agcgagccgc
     3481 ccgagttgtt acaaataccg ccgatgaccg atgcgcctat acacgatgat ccaatcactg
     3541 agtgcggttc gcgtcccagc ggtttgaggg ctttttccag tgaatagagc gtggtgcccg
     3601 gataggccag cacttgttcg cccttgccga gaacgtgcag cttgtcgaga cgcagggtgc
     3661 tgatgatcac gatatcgcga tcataatcgt taccgtttgg cgtcgatcct tcggtcaggc
     3721 ctgtattggc agcctgcatc agaataattt tgtcagcgct gacgcaggct ttcagcaccc
     3781 gccacaattc cagtagtgag ccagggaaaa cgacagccag cgcgtcgccc tgaccagaac
     3841 ggaagccctt gcgatagcgg gccgtttttg cgggatcggt gagcaggtgt gaatgaccga
     3901 ccagacgggc aagttcattc aaaaaggctt tattatcagt tgttgtcatg gaagacattt
     3961 tccactcctt gtggtggcga aaaaatatca caaactagaa tagcgccttg tatgacaatt
     4021 cagcgagtga atattttgaa aattcagaca ataaactgag cattcttttt ctctctccat
     4081 catgcttatg gcaaactacg gtttttgcac cattttcgct gccacgtcgg gcaacaaagg
     4141 aagaaaaatc catatgaaat ggctatgttc agtaggaatc gcggtgagtc tggccctgca
     4201 gccagcactg gcggatgatt tattcggcaa ccatccatta acgcccgaag cgcgggatgc
     4261 gttcgtcacc gaactgctta agaaaatgac agttgatgag aaaattggtc agctgcgctt
     4321 aatcagcgtc ggcccggata acccgaaaga ggcgatccgc gagatgatca aagacggtca
     4381 ggttggggcg attttcaaca ccgtaacccg tcaggatatc cgcgccatgc aggatcaggt
     4441 gatggaatta agccgcctga aaattcctct tttctttgct tacgacgtgc tgcacggtca
     4501 gcgcacggtg ttcccgatta gtctcggtct ggcttcgtct tttaacctcg atgcggtgaa
     4561 aacagtcggg cgtgtctctg cttatgaagc ggcagatgat ggcctgaata tgacctgggc
     4621 accgatggtc gatgtctcgc gcgatccgcg ctggggacgt gcttccgaag gttttggcga
     4681 agatacgtat ctcacctcaa taatgggcaa aaccatggtg gaagcgatgc agggtaaaag
     4741 cccggcagat cgctactcgg tgatgaccag cgtcaaacac tttgccgcat acggcgcggt
     4801 agaaggcggt aaagagtaca acaccgtcga tatgagtccg cagcgcctgt ttaacgacta
     4861 tatgccgccg tacaaagcgg ggctggatgc aggcagcggt gcggtgatgg tggcgctgaa
     4921 ctcgctgaac ggcacgccag ccacctccga ctcctggctg ctgaaagatg ttctgtgcga
     4981 ccagtggggc tttaaaggca tcaccgtttc cgatcacggc gcaatcaaag agctgattaa
     5041 acatggcacg gcggcagatc cagaggatgc ggtacgcgtg gcgctgaagt ccggaatcaa
     5101 catgagcatg agcgacgagt attactcgaa gtatctgcct gggttgatca aatccggcaa
     5161 agtgacgatg gaagagctgg acgatgctgc ccgccatgta ctgnnnnnnn nnnttaaata
     5221 tgatatggga ttgtttaacg atccatacag ccatttgggg ccgaaagagt ctgacccggt
     5281 ggataccaat gccgaaagcc gcctgcaccg caaagaagcg cgtgaagtgg cgcgcgaaag
     5341 cctggtgttg ctgaaaaatc gtctcgaaac gctgccgctg aaaaaatctg caaccattgc
     5401 ggtggttggc ccgctggcag acagcaagcg tgacgtgatg ggaagctggt cggcggcagg
     5461 tgtcgccgat caatctgtta ctgtgctaac cgggattaaa aacgccgtcg gtgaaaacgg
     5521 taaagtgctg tatgccaaag gggcgaacgt taccagtgac aaaggcatta tcgatttcct
     5581 gaatcaatat gaagaggcgg tcaaagttga tccgcgttcg ccgcaagaga tgattgatga
     5641 agcagtgcaa accgcgaaac aatctgatgt ggtggtggct gtggtcggtg aagctcaggg
     5701 gatggcgcac gaagcttcca gtcgtaccga tatcactatt ccgcaaagcc aacgtgactt
     5761 gattgcggcg ctgaaagcca ccggtaaacc gctggtgctg gtgctgatga acgggcgtcc
     5821 gctggcgctg gtgaaagaag atcagcaggc tgatgcgatt ctggaaacct ggtttgcggg
     5881 gactgaaggc ggtaatgcaa ttgccgatgt attgtttggc gattataacc cgtccggcaa
     5941 gctgccgatg tccttcccgc gttctgtcgg gcagatcccg gtgtactaca gccatctgaa
     6001 taccgggcgt ccgtataatg ccgacaagcc gaacaaatac acttcgcgtt attttgatga
     6061 agctaacggg gcgctttatc cgttcggcta tgggctgagc tacaccactt tcaccgtctc
     6121 tgatgtgaaa ctttctgcgc cgaccatgaa gcgtgacggt aaagtgacgg ccagcgtgca
     6181 ggtgacgaac accggtaaac gcgaaggggc cacggtagta cagatgtact tgcaggatgt
     6241 gacggcttcc atgagtcgcc cggtgaagca gctgaaaggc tttgagaaaa tcaccctgaa
     6301 accgggcgaa actcagaccg tcagcttccc aatcgatatt gaggcgctga agttctggaa
     6361 tcaacagatg aaatatgatg ccgagcctgg caagttcaat gtcttcatcg gcactgattc
     6421 cgcacgcgtt aagaaaggcg agtttgagtt gttgtaattt tcgatgccgg ataaggcgtt
     6481 tacgccgcat ccggcaagaa acgccgggca aaatatgcct tttaagtatt tatctgattc
     6541 tgcaactgag tccatccagt caggctaact ccgccattat caactatgct tttctcttaa
     6601 ttcgctgaaa acaacagtaa aagaatgagg aaagcatcat gccactctca aaggtctggg
     6661 caggttcact ggttttgttg gcagccgtga gcctgccgct acaggcggct tcccccgtta
     6721 aggttggttc aaaaatcgat acggaaggcg cgctgctcgg cannnnnnnn nncaggtgct
     6781 ggaaagtcac ggcgttccta cggtaaataa agtgcagctg ggaacgactc ccgtagtgcg
     6841 cggggcgatc acttctggcg agctggatat ctatccggaa tatactggca atggcgcgtt
     6901 tttctttaaa gatgaaaacg atacggcatg gaaaaacgcg cagcaaggct atgagaaagt
     6961 caaaaaactc gatgcagagc aaaacaagtt aatctggctg acgcccgcgc ctgcaaataa
     7021 cacctggacc atcgccgtgc gtcaggatgt ggcagagaaa aacaaactca cttcgcttgc
     7081 cgagctgagt cgttatctga aagagggtgg caacttcaaa ctggcagcct cggcagagtt
     7141 tattgaacgc gccgatgcct tacccgcgtt tgaaaaagcc tatggcttta agctcggtca
     7201 ggatcagttg ctgtcactgg ccggtggtga cacggcggtg acgatcaaag ccgctgccca
     7261 gcaaacttct ggtgttaatg ccgcaatggc ttacggcacc gacggcccgg ttgcggcgct
     7321 ggggctgcaa accttaagcg atccgcaagg cgtgcaacct atctacgcgc ctgcgccagt
     7381 ggtgcgtgag tcggtgctga aagagtatcc gcaaatagca cagtggctac agccagtctt
     7441 cgccagcctc gatgaaaaaa cattgcagca gctgaatgcc agcattgcag tggaaggact
     7501 ggatgccaaa aaagtggcag ccgactacct gaaacaaaaa gggtggacga agtaatttcc
     7561 cgtgacttat ctacgtatta atcctgttct ggcgctgctg ctgttgctga cggcaatcgc
     7621 agcggcgctg ccgtttatca gttacgcacc taatcgttta gtttcgggtg aggggcgtca
     7681 tctctggcag ctgtggccgc aaactctctg gatgctggtg ggcgttggtt gcgcctggct
     7741 gacagcctgt tttattcccg ctaaaaaagg cagcattttt gcactcattc tggcgcaatt
     7801 cgtcttcgta ttgctggtgt ggggagctgg aaaggcggcg acacaactgg cgcaaaatgg
     7861 cagtgcgctg gcgcgtacca gcctcggcag tggtttctgg ctggctgcgg cgctggcact
     7921 gctggcctgt agcgatgcca tccgccgaat ctccacgcat ccgctgtggc gctggttgtt
     7981 gcatatgcag attgccattg ttccgctgtg gttgctgtac tccggcacgc ttaacgatct
     8041 ctcgttaatg aaagaatacg ccaaccgtca ggatgtgttt gacgacgcgc tggcacaaca
     8101 tctgacgttg ctgtttggtg cggtgctgcc tgcgttagtg attggtgtgc cgttgggcat
     8161 ctggtgctac ttttccaccg cgcggcaggg ggcgattttt tctctgctga atgtcattca
     8221 gaccgtgcct tcggtggcgc tctttggcct gctgattgcg ccgcttgccg cgctggttac
     8281 ggcctttccg tggctgggaa cgctcggcat agcaggaacc ggaatgacac ccgcacttat
     8341 tgcgctggtg ctctatgcct tgctgccgct ggtgcgcggc gtggtagtcg gcttgaacca
     8401 gatcccgcgc gatgtgctgg agagcgccag agcgatgggg atgagcgggg cgcagcgttt
     8461 cctgcatgtt cagttgccgc tggcgttacc ggtatttctg cgcagcctgc gggtggtgat
     8521 ggtgcaaacc gttggcatgg cggtgattgc ggcgttaatc ggcgcaggcg gttttggtgc
     8581 gctggttttt caggggctgt taagcagtgc cattgattta gtgttgctgg gggtgatccc
     8641 ggtaattgtt ctggcggtgc ttaccgacgc gctgttcgat ttgcttatcg cactgctgaa
     8701 ggtgaaa
//
LOCUS       DAWDRC010000090         8696 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3981_length_8696_cov_2.310149, whole genome shotgun sequence.
ACCESSION   DAWDRC010000090 DAWDRC010000000
VERSION     DAWDRC010000090.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3981_length_8696_cov_2.310149"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2228 a         2352 c         2056 g         2060 t
ORIGIN      
        1 ttagtattta acaataaccg aatataaaac aaccatttcc gactcaacaa tcgcttacac
       61 tcgcttatat attgaccaca actgatacat cagattatgt gacgacttgt gcctagatca
      121 attttggcaa tcattagcaa caagattaat aaaccatcta tatcaattta cctatcctat
      181 tttaccgttc aagaattcac cgaacggtta tttttaacaa atttttctct tcccattgac
      241 tttcccggac accttgtctg acctaaggtg cgcggaagcc actttttcct tcctgagtta
      301 tccacaaagt tatgcacttg caagagggcc agtttcacac tatcttgcag tgaatcccaa
      361 acataccccc tatatatagt gttcttagca gcttccctca ctacaggtag tctgcatgaa
      421 gctattgaaa aacaggtacg acatacatga atcagaatct gctggtgaca aagcgcgacg
      481 gtagcacaga gcgcatcaat ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag
      541 gactgcataa cgtttcgatt tcccaggtcg agctgcgctc ccacattcag ttttatgacg
      601 gtatcaagac ctctgacatc cacgaaacca ttatcaaggc tgccgcagac ctgatctccc
      661 gtgatgcgcc ggattatcag tatctcgccg cacgtctggc gatcttccac ctgcgtaaaa
      721 aagcctacgg tcagtttgag ccgcctgcgc tgtacgacca tgtggtgaaa atggtcgaga
      781 tgggcaaata cgataatcat ctgctggaag actacacgga agaagagttc aagcagatgg
      841 acacgtttat cgatcacgac cgtgatatga ccttctctta tgctgccgtt aagcagctgg
      901 aaggcaaata tctggtacag aaccgcgtga ccggcgaaat ccacgaaagc gcccagttcc
      961 tttatatgct ggttgccgcg tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat
     1021 acgtgaagcg tttttacgac gcggtttcca catttaaaat ttcgctgccg acgccaatca
     1081 tgtccggcgt gcgtaccccg actcgtcagt tcagctcatg cgtgctgatc gagtgcggcg
     1141 acagcctgga ttccattaac gccacctcca gcgcgattgt taaatacgtt tcccagcgtg
     1201 ccgggatcgg catcaacgcc gggcgtattc gtgcgctggg tagcccgatt cgcggtggcg
     1261 aagcgttcca taccggttgt attccgttct acaaacattt ccagacagcg gtgaaatcct
     1321 gctctcaggg cggtgtgcgc ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg
     1381 aagtggaaag cctgctggtg ttgaaaaaca accgtggtgt ggaaggcaac cgcgtgcgtc
     1441 atatggacta cggggttcaa atcaacaaac tgatgtatac ccgtctgctg aaaggtgaag
     1501 atatcactct gttcagcccg tccgacgtac cggggctgta tgacgcgttc ttcgccgatc
     1561 aggaagagtt tgaacgcctg tataccaaat atgagaaaga cgacagcatc cgcaagcaac
     1621 gtgtgaaagc cgttgagctg ttctcgctga tgatgcagga acgtgcgtct accggtcgta
     1681 tctatattca gaacgttgac cactgcaata cccatagccc gtttgatccg gccatcgcgc
     1741 cagtgcgcca gtctaacctg tgcctggaaa tcgccctgcc gaccaaaccg ctgaacgacg
     1801 tcaacgacga gaacggtgaa atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa
     1861 ttaacagcct ggatgaactg gaagagctgg caattctggc agtacgcgca ctcgacgcgc
     1921 tgctggatta tcaggattac ccgatcccgg ccgccaaacg tggagcgatg ggtcgtcgta
     1981 cgctgggtat tggtgtgatc aacttcgctt actacctggc gaagcacggt aaacgctact
     2041 ccgacggcag cgccaacaac ctgacgcata aaaccttcga agccattcag tattacctgc
     2101 tgaaagcgtc taatgagctg gcgaaagagc aaggcgcgtg cccgtggttt aacgaaacca
     2161 cttacgcgaa agggatcctg ccgatcgata cctataagaa agatctggat gccatcgcta
     2221 atgagccgct gcattacgac tgggaagcac tgcgtgagtc aatcaaaacg cacggcctgc
     2281 gtaactccac gctttctgcc ctgatgccgt ccgagacttc ttcgcagatc tctaacgcca
     2341 ctaacggtat tgaaccgccg cgcggttacg tcagcatcaa agcgtcgaaa gacggtattc
     2401 tgcgccaggt ggtgccggac tacgagcatc tgcacgacgc ctatgagctg ctgtgggaaa
     2461 tgccgggtaa cgatggctac ctgcaactgg tgggtatcat gcagaaattt atcgatcagt
     2521 cgatctctgc taacaccaac tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc
     2581 agcagttgct gaaagacctg ctcactgcct acaaattcgg ggtcaaaacg ctgtattatc
     2641 agaacacccg tgacggcgca gaagacgcgc aggacgatct ggtgccgtca atccaggacg
     2701 atggctgcga aagcggcgca tgtaagatct gatattgtga tgccggatgc ggcatgaacg
     2761 ccctacccag cctacgatgc ggtttgtagg ccggataagc gaagcgcatc cggcataaaa
     2821 ctcctaacag gacacattca tggcatatac caccttttca cagacgaaaa atgatcagct
     2881 caaagaacca atgttctttg gtcagccggt caacgtggct cgctacgatc agcaaaaata
     2941 tgacatcttc gaaaagctga tcgaaaagca gctctctttc ttctggcgtc cggaagaagt
     3001 tgacgtctcc cgcgaccgta tcgattacca ggcactgcct gaacacgaaa aacacatctt
     3061 tatcagcaat ctgaaatatc agacgctgct ggattccatt cagggtcgca gtccgaacgt
     3121 ggcactgctg ccgctgatct cgatcccgga actggaaacc tgggtagaaa cctgggcgtt
     3181 ctcagaaacg attcattccc gctcctatac ccatatcatc cgtaatatcg ttaacgatcc
     3241 gtctgttgtc tttgacgata tcgttaccaa cgagcagatc cagaaacgtg cggaggggat
     3301 ctccagctat tacgatgaac tgatcgaaat gaccagctac tggcatctgc tgggcgaagg
     3361 cacccacacc gttaacggta aaaccgtaac cgttagcctg cgcgagctga agaaaaaact
     3421 gtatctctgc ctgatgagcg ttaacgcgct ggaagcgatc cgcttctacg tcagctttgc
     3481 ctgctctttc gcattcgctg aacgtgagct gatggaaggt aacgccaaaa ttattcgcct
     3541 gattgcccgc gacgaagccc tgcacctgac cggcacccag catatgctga atctgctgcg
     3601 cagcggcgcg gatgatccgg agatggcgga aattgccgaa gaatgtaagc aggagtgcta
     3661 tgacctgttt gtccaggcgg cacagcaaga gaaagactgg gcggattatc tgttccgtga
     3721 tggttcgatg attggtctga ataaagacat cctctgccag tacgttgaat acatcaccaa
     3781 tatccgtatg caggcagtcg gtttggatct gccgttccag acgcgctcca acccgatccc
     3841 gtggatcaac acctggctgg tgtctgataa cgtgcaggtt gctccgcagg aagtggaagt
     3901 cagttcttat ctggtcgggc agattgacgc ggaagtggac accgacgatt tgagtaactt
     3961 ccagctctga tggcccgcgt taccctgcgc attactggca cacaactgct gtgccaggat
     4021 gaacaccctt cgcttctggc ggcgctggaa tcccacaatg tggcggttga gtaccagtgc
     4081 cgcgaaggtt actgcggctc ctgtcgcacc cgactggtcg caggccaggt ggactggatt
     4141 gccgaaccgt tagcttttat tcagccgggg gaaattttgc cctgttgttg ccgggcaaaa
     4201 ggcgatattg aaatcgagat gtgaattggt tttaacgcca gatacgacgc ttatacgtct
     4261 tatctggcct acaacgatta catagcgtag taatatgctt tcacctgctc ccagtccgct
     4321 ttggggatcg gctccagata tttttccagc tggcggaagt catgattaat cgctttatca
     4381 cgacgcaagc ggcgacgact tttctccaga tcaagaaaac cagcttctgc cttaccttct
     4441 gttttcacat agatatggcg aacataacaa cagccatgct gacgattaac gctatgcatt
     4501 ttcttaaacg ccagcgccac tgctttcaac atggcttgcc gtacttcgtc agaataaggc
     4561 gatactgcat gccgggcata ccagtcagca atgctgatga accccgccat atcttcagtc
     4621 accagcaacg cgcgccattc accctcaact ttcaccgctt caccaaaaac gatcttcggc
     4681 acgatgacac cggcccgttc cagttcttta attaccgcaa cctcacggac aatcgttggt
     4741 cggccgaacg gataacgtac ggaatgaaac agatgatgcg tcatgcgctt tacatacagc
     4801 tttttgccgt tgcgctcgac gcattgcacc ccgctcatac cattacggcg atagttaggc
     4861 tcttcaaccc agtcgccctc tgttgcccac cagtgattaa attcgtcgtg ttttgctgaa
     4921 actgccatac cctatcgcct gtcgtttttt attaacgaca atgactatag atgtttagct
     4981 gaggaaaatc ttaataatac gtgttgtatt gacgagtatc ttatgccggg aacaagtcat
     5041 ctcgctcaaa ataattgcaa ttaaggataa cccacagact ttaatgaaga gtggtatcgg
     5101 atagcacata agtgccatcc ggtagttaat ttactcttta ttaaggaatt ttactgcctt
     5161 atcagggaaa tcagtaaata gcccatttac acccgctttg ttatacagag catcatataa
     5221 ctgattcaca tcagtagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac
     5281 taccagttta ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg
     5341 cgatgtctcc tcaatcaaca tatggtaatc cggaccaata ccatcagcat attccgccac
     5401 ctgtttcata gcccccggct taaacatcca gtcgtagttg taattaaccc agcttccgtc
     5461 cggctgtttc tgctgcgttt cgttccagtc ggtataggca atcagttgta ccagattgag
     5521 atccataccc attttaggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa
     5581 acattgcaaa taaactttgt cgtccttacc ggtgtaacca tatttcttca gcacttccag
     5641 cgtttttgcc gcaatatcct tcccttcctg atgatggaac cacggcgctt taatttctgg
     5701 atagataccg atatttttcc cggtagagtg gtttaacccc tgaacaaatt caatctcttc
     5761 ttcaaaggtg tgcacccgga agtcagattt acccattggg aaacgccccg gataagtctg
     5821 cactttttta ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc
     5881 cagcgtgaaa tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc
     5941 ggcaacatca gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt
     6001 ggtcatcacc aaatcctgtt ccagataatc cgctccctgc gcatacgcca tcgcttttgc
     6061 tggcagcgta tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc
     6121 gttgctgtcc gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat
     6181 gctaaggttt ttcagcgtca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt
     6241 gttcatggcg acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc
     6301 tgccgccaat cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa
     6361 caatcgcgct cgccgccacc gaaccgccga ggtaaccaaa cagcccggta aaacccgctg
     6421 ccgtacctgc cgcttttttc ggtgccagtt ccagcgcatg tagaccgatc agcatcacag
     6481 ggccatagat caggaagccg ataacaatca tacaaatcat atcgacggtt ggattacctg
     6541 ccgggttcat ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg
     6601 ttgccccacg gttaccacga aagactttat ccgacatcca gccgcacagc agggtgcccg
     6661 gaatacctgc atattcataa agaaagtagg cccaggagga tttatccagc gcgaaatgct
     6721 taacctcttt cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga
     6781 acacgttggc gatggcgata taccacagca gtttgttcgg cagcacgtac tgcatgaaga
     6841 tctgcttcgc ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt
     6901 tgtactcttc gatcggcggc aggccacagg attgcggggt atcgcgcatc atcgcaaagg
     6961 cgaacagtgc caccagaatg gcgcagaaag cgggcatata gagcgccgcg tgccagtcat
     7021 tgaaccaggc catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg
     7081 cacagttcca cactgacaca ataccgccac gttctttctg cgaccaccag tgcaccatag
     7141 tacgaccaca cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa
     7201 acatcaccgc aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg
     7261 ccgccagaaa caaaactgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac
     7321 ccatgatgaa tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat
     7381 caccgcgtga gaatccctgc tcaaccagat aaggcatagc gagcgcaaag ttcttacgaa
     7441 ccaaatagta agcagcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac
     7501 aataagtcgg atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa
     7561 tactcaacat tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca
     7621 gtgggagaac ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa
     7681 tgtgaattac cgcacacatt attaaatacg atggttgaaa tgttcagaat gacgcatgaa
     7741 atcacgtttc actttcgaat tgtgagcgaa tatgcgcgaa atcaaacaat tcatgttttt
     7801 actatggcta aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg
     7861 caatcaagtg acgtgattat cattggcggc ggcgcaacgg gagccggaat tgcccgcgac
     7921 tgtgccctgc gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc
     7981 accgggcgta accacggcct gctgcacagc ggtgcgcgct atgcggtaac cgatgcggaa
     8041 tcggcccgcg aatgtattag tgaaaaccag atcctgaaac gcattgcacg tcactgcgta
     8101 gaaccaacca acggcctgtt tatcaccctg ccggaagatg acctctcctt ccaggccact
     8161 tttatccgcg cctgcgaaga agcagggatc agcgcagaag ccattgatcc gcagcaagcg
     8221 cgcattatcg aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc
     8281 accgttgatc catttcgtct gacagcagca aacatgctgg atgccaaaga acacggtgcc
     8341 gttatcctta ccgctcatga agtcacgggg ctgattcgtg aaggcgcggc ggtgtgcggt
     8401 gttcgtgtac gtaaccatct caccggcgaa attcaggcac ttcatgcgcc tgtcgtagtt
     8461 aatgccgccg ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg
     8521 ttcccggcga aaggatcgct gctgatcatg gatcaccgca ttaatcagca tgtgatcaac
     8581 cgctgccgca aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt
     8641 ggcaccacct ctttacgtat tgattacaac gagattgacg ataatcgagt gacggc
//
LOCUS       DAWDRC010000091         8670 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_3989_length_8670_cov_2.450029, whole genome shotgun sequence.
ACCESSION   DAWDRC010000091 DAWDRC010000000
VERSION     DAWDRC010000091.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8670)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3989_length_8670_cov_2.450029"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    960..969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3057..3066
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4804..4813
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6511..6520
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2104 a         2227 c         2210 g         2089 t
ORIGIN      
        1 cgcgtcttat caggtctaca ggcggtgtcg gaacggtagg ccggataagg cgtttacgcc
       61 gcatccggca agaaaatgag catcaattaa ctgaacttgc tctgcgccca catcacgccg
      121 ctggcatact ccggcggcag cagtgggatt aaggcttcca gcgtcgcggt cagacgcgat
      181 gtgtcgctgt cggtcaaatt taaatgcccc actttacggc ccggacggac ttctttgtcg
      241 taccagtgca gatgcacaag cggcagtttc agccagtcat aattcagatc gctaccaatc
      301 agattgatca tcaccgacgg attattcact actggttgcg gtaacggcag atcggtaatc
      361 gcccgcagat gtagctcaaa ctggctgatg ctggcaccgt tttgcgtcca gtgcccactg
      421 ttatgcacac gcggagccag ttcgttgatc agcagacctt gtggggtgac aaaacactcc
      481 attgccatca cgcctacata gctcagctcc tgcataatcg ccgacaacat ctcttcagct
      541 tgcgcctgca gctgtgcgtt ggcctgcggg aaagcgacgc tggtgcgcaa aatgccgtcc
      601 tgatgcaggt tatgcgtcag cggataaaac acggtgctac catcaaagcc gcgcgcgcca
      661 accagcgaca cttcaccaga gaagtttatg ccctgctcga caatacattc gccgtagcat
      721 tcagccggta actgttcggt ttcagcggcg cgtaaacgcc attgaccgcg tccgtcatag
      781 ccaccagtgc ggcgcttaac aatcgccagc tcacctaaac gatcaaacac cgcaggccac
      841 tcgctgcgtt cggcaagtaa ctgccacggt gcggtcggca ggtggagttt atcgaaaagc
      901 tgcttctgag tcagacggtc ggcaataatc gggaacacat cgcggttcac aaaggccggn
      961 nnnnnnnnnc ccgaccggcc agacagcaat gcctaacggt tcgcctgcct gacgtagcat
     1021 acggcctaac tgcccgttcc cgaggacgca aacctgtttc atgccgcacc tcgcgggtcc
     1081 gggttttcca ggacttcatc agtttgtgct ttgcgccagt cattcagacg ctggtgcagt
     1141 tctttatcat gagtcgcaag aatttgtgct gccagtaacg ccgcgtttgc cgcgccagct
     1201 ttaccaatcg ccagcgtacc caccggaatg ccgcgcggca tttgtacgat ggagtagagg
     1261 ctatcgacac cgctcagtgc ggcgctctgt actggcacgc ccagcaccgg caccagcgtt
     1321 ttggcggcaa tcatgccagg caggtgcgct gcgccgcctg cgcccgcaat aatcacctga
     1381 taaccgttct cttcggcgct ttcggcgaag ctgaacagtt tatcgggggt gcggtgagca
     1441 gaaaccactt caacgtggtg cgggacattc aggatttcga agatttcggc ggcgaactgc
     1501 atggtagccc agtcgctttt ggaccccatc acgatggcga cacgcgccgg attattgcgg
     1561 gaagacatgc gtcttaaaac tcctgtggtg cacaactctc ggctttagag ggcacagaga
     1621 atagcacgga aagagtgcaa ggaaaacggt tgcgtaggcg gaattaaccg tcagttgttg
     1681 agtttttaat ttgttctgcg tgttgattat gcgctctggc tgaactggca aagaatgccc
     1741 atatcaacac aatgctccat ggaaaaaagc aaataccgac aatcaatata aatctttcta
     1801 caaaacgttg gttatggggg cgggtgagta ctaaataaat gagccagaaa tagactatcc
     1861 aggaaatgac cgccgcagct accagtgaca atgcgccaaa atagaagccg acaagaccga
     1921 accagctaat acacagtggc gctcctgccg taagggcgat gccagaaatc aaaaatccgt
     1981 tgcagcgttg cataataata ttccctgttg ttatttcact cagagattat taccttgctg
     2041 attttaaaaa agatatacag gttgcgttag ttcggtgctt tttcagaatc tgtccattcg
     2101 tggtcagaaa ggaaaatgga ttagctcaac gtcatccgcc gtgactttca ccatcgaacc
     2161 ttctgtatgc caggcaccca gtaccacgcg aaaagcaggt tgctgattgg cgataagttc
     2221 atgcaccgcc gggcgatggg tatgcccgtg gatcagccat tgcacttgat gtttttccat
     2281 cgcactgacc accgcgtttt ggttaacgtc catgatcgcc agcgatttac tgctgttggc
     2341 ttctttactg ttcgcgcgca ttcgcgcggc aatgcgtttg cgcacaaaca acggcagggc
     2401 gaggaatagc gtttgcagcc agggtttgtg gactttggcg cgaaaagcct gataacctgc
     2461 gtcatcggtg cacagcgtgt cgccatgcat aatcaacacc cgacgaccat aaagtttgag
     2521 caccttttct tccggcaata acgtcatgcc gctttcacgg gcaaagcgtt tgccgagcag
     2581 aaaatcacgg ttgccatgaa tgaaataaca gggaacgccg gaatcggata ccgctttgat
     2641 cgctgcggcc atctggtgat gaagtgggtt gggatcgtcg tcgccgatcc aggcttcaaa
     2701 cagatcgcca agaatataca gcgcatcggc cttgcgggcc tcccccgcta aaaaacgcag
     2761 aaaaccggcg gtgatcgccg gttcttccgc gcagagatga agatctgcaa taaagagtgt
     2821 cgccacgatt actcgctaac ggtcacgctt tcaatgataa cgtcttcttt tggcacgtcc
     2881 tggtgcatac cgctacgacc ggttgccaca cctttgattt tgtctaccac gtccatgcct
     2941 tcaaccactt cagcaaatac gcagtagccc caaccttgca ggctttcgcc agagaagttc
     3001 aggaagtcgt tatcaaccac gttgatgaag aactgggcag ttgcagagtg cggtgcnnnn
     3061 nnnnnngtgc ctgagtacgg gccattgcca gcgtaccacg ggtatttttc agaccgttgt
     3121 tggcttcgtt tttgatcggt tctttggtgg ctttttgttt catgcccggt tcaaaaccgc
     3181 cgccctgaat cataaagccg ttgataacac ggtggaaaat ggtgttgttg taaaaacctt
     3241 cgcggcagta gtccaggaag tttttaactg tttcaggtgc tttatcgtca aaagttttga
     3301 tgacaatatc gccgtgattg gtgtggaaag taaccatttt tgcatcctgt tccgtttgat
     3361 tggtgcttca acccggttcg ggtcatatat agggtggtgt tatagcataa ccgcacgatc
     3421 ggatcatctc gcaatgtatg ctgattcgcg cgggaaatat gggtattata cgcaactcaa
     3481 ttacccacac atgtctaaac ggaatcttcg atgctaaaaa tcttcaatac tctgacacgc
     3541 caaaaagagg aatttaagcc tattcacgcc ggggaagtcg gcatgtacgt gtgtggaatc
     3601 accgtttacg atctctgtca tatcggtcac gggcgtacct ttgttgcctt tgacgtggtt
     3661 gcgcgctatc tgcgtttcct cggctataag ctgaagtatg tgcgcaacat taccgatatc
     3721 gacgacaaaa tcatcaaacg cgccaatgaa aatggcgaaa gctttgtggc gctggtggat
     3781 cgcatgatcg ccgaaatgca caaagatttt gatgctttga acattttgcg cccggatatg
     3841 gagccgcgcg cgacgcacca tatcgccgaa attattgaac tcactgaaca actgatcgcc
     3901 aaaggtcacg cttatgtggc ggacaacggc gacgtgatgt tcgacgtccc gaccgatccg
     3961 acatatggcg tgctgtcgcg tcaggatctc gatcagctgc aggcaggcgc gcgcgttgac
     4021 gtggtcgacg acaaacgcaa cccgatggac ttcgttctgt ggaagatgtc gaaagagggc
     4081 gaaccgagct ggccgtctcc gtggggcgcg ggccgtcctg gctggcacat tgagtgttcg
     4141 gcaatgaact gcaaacagtt gggtaaccac tttgatatcc acggcggcgg ttctgacctg
     4201 atgttcccgc accacgagaa cgaaattgcc cagtccacct gtgcgcacga tggccagtat
     4261 gtgaattact ggatgcactc cggtatggtg atggttgacc gcgagaagat gtctaaatcg
     4321 ctgggtaact tcttcaccgt gcgtgacgtg ctgaaatact acgatgcgga aactgtgcgt
     4381 tacttcctga tgtcgggtca ctatcgcagt cagttgaatt acagcgaaga gaacctgaaa
     4441 caggcgcgtg cggcgctgga acgtctctat actgccttgc gcggcacaga caaaaccgtt
     4501 gcgcctgctg gtggcgaagc gtttgaagcg cgctttattg aagcaatgga cgacgatttc
     4561 aacaccccgg aagcctattc cgtactgttt gatatggcgc gtgaagtaaa ccgtctgaaa
     4621 gcagaagata tggcagcggc gaatgcaatg gcgtctcacc tgcgtaaact ttctgccgta
     4681 ttgggcctgc tggagcaaga accggaagcg ttcctgcaaa gcggtgcgca ggcagacgac
     4741 agcgaagtgg ctgagattga agcgttaatt caacagcgtt tggatgcccg taaagcgaaa
     4801 gacnnnnnnn nnnggggatc gtgctggaag atggcccgca agggaccacc tggcgtcgta
     4861 agtaattgcg ctattgccgg atgcgagttt tcgcatccgg ttatcgtctg cgccaccaca
     4921 acattcccat cagtagcatc cccggcaacc acacccacat caattcagaa ataatcacct
     4981 gatgcccgta cggcgtggtg taacgagaca atgcaaacgg cgcgacttta atcacctgcc
     5041 agggagcgaa aaagcgttca tctgaccacg gccacagcca gccaacccct ttaccgccag
     5101 tggttaccga atccagcaag ctgtgcgata gcaacgagac ggttaaaaac agccagcagc
     5161 gaatcagccc agccctgaac catcgacgtc caatcagcac acataacagc gggacaacaa
     5221 acgcaaacac cagcgaatgg gtaaacccgc gatgaccaaa aacattgccg taagcaacgc
     5281 caaatttaaa cgacaataca tcagcatcgg gcagcatcgc caggatgatt ccggcaaata
     5341 acagacgcgg agggatggct ttcgaaccca accctaaacc aatgcatagg ggaacggcgg
     5401 cgtgcgtaat aacggttggc atgatggtcg cttcggcaaa atgtcgatgc tatcagcatg
     5461 gatgaacggg gcgtagaggg caaaagtctg aaaagagaac cggcctgttg atacaggccg
     5521 ggaaagggat caggcaacaa cctgtacgct gtgacctgca aaactcactg tctgaccggc
     5581 gacaattttg cagcgtttgc gcgtttcaac cgcaccgtcg actttcactt ggccttctgc
     5641 tatcgcgatt ttcgcctgcg cgccgctttc gctccagcct tccagtttca gcaagtcgca
     5701 cagctcaacg tgcggatgtt tacctaaaga aaatgtcgcc atgttactca ccctgtggat
     5761 catgatattc aacgcacgcc tgtagcgtgt tttcaatcag cgtggcaacc gtcatcgggc
     5821 caacgccgcc cggaacaggc gtaatgtatg aggcgcgttt agccgcgtct tcaaacacga
     5881 cgtcgcccac aactttgcca ttttccagac ggttgatgcc gacatcaatc acaattgcgc
     5941 cttctttgat ccagtcacca ggaataaagc ccggcttgcc gacggcaacg atcagtagat
     6001 cggcattttc tacgtggtga cgcagatttt tagtgaaacg gtgggtcacg gtagtggtgc
     6061 aacctgccag cagcagttcc atgctcattg ggcgaccgac gatattcgat gcgccaatca
     6121 ccacggcgtt aaggccaaag gtatcaatgt tgtaacgctc aagcagcgtg acgataccgc
     6181 gcggggtgca gggacgcaga cgtggcgcgc gctggcacag acgaccgacg ttgtaaggat
     6241 ggaaaccgtc cacgtctttg tccggatgaa tacgttccag cactttgacg ttatcaatac
     6301 ccgccggtaa cggcagttga accagaatgc catcaatggt gttgtcggca ttcagcgcat
     6361 cgataagctc cagcagctcc gcttcgctgg tggtttccgg gaggtcataa gagcgggaga
     6421 cgaacccgac ttcttcacaa gccttgcgtt tgcttgcgac ataaatttgc gatgcagggt
     6481 tactgcccac cagcacaacg gccagtcctg nnnnnnnnnn ggctgcaata cgcgcctgaa
     6541 ctttttgagc aacttcagag cgcacctgct gcgcaatcgt tttaccgtca ataatctttg
     6601 ctgccatcag agagaggatt ccttctgtta cgtaggtcga aggggatgtg tctattttgt
     6661 cagaagcgtg gcgcgctgtc agttttcgtt ttctgaaaag taaaaccttc tgtacctggc
     6721 tgattcacac ctgtcatgat aaccgtcgtt ggtttgccgc catagtgagc tacatcagat
     6781 gcggtcgtac acttgttcag caactggttc aaaagccatt gactcagcaa gggttgaccg
     6841 tataattcac gcgattacag cacaccgtgg tatcaatgcg cccttagctc agttggatag
     6901 agcaacgacc ttctaagtcg tgggccgcag gttcgaatcc tgcagggcgc gccattaaaa
     6961 ttcaatcagt tacgccttct ttatttcttc tataattcca gagtgggaca ttatcaccaa
     7021 aaatgtcgtc tattttcctc gcgtgctcag tcaaatgatt aggcgcaagg tgagcatatc
     7081 tacgaaccat ttctctggac tcccatccgc ccatttcctg aagcactgat aatgggacgc
     7141 ctgacggaat caacctgctt gcccgggtat gccagaggtc atggaaacgg aaattttcaa
     7201 ttcctgcacg acgacaagct gatagccatg ctgtcttgct gtcaatgcgc atcttcctga
     7261 gtgcaggcat tgatgttcca tctgctcgct tagccgccct ggtatgtaca cccacatttg
     7321 tgatgcttgc ctgtttgatc acgcaacact ttagaggcgg tattgttcag cgccacacca
     7381 atggcgcagt ttgatttgct atcttctgga ttcaccctgg caacttgtcg ctgcatgtca
     7441 atctgttgcc actccagatt tatgatgttc gactttctca gaacagttac cagcgcaaat
     7501 ttgacgacag atttcagtgg ttcggggctc tcatcaatca ggcgtttggc ctgattcttt
     7561 tgtaagtctc ttgtgccatt ccacctcata caggtttgcc tgcaaacagt actgatattg
     7621 cccagatatt ccggctggct tcgtggttgt tttcaacagt gatgaggcat cgtggcatct
     7681 cgttgaagac catcggggta aaacggttta tgacgtggca tcaggggacg cgttatttat
     7741 ttctgagctc ggtccgttac cggaaaatgt tacctggtta tccccggcag gggagtttca
     7801 gaagtagaac ggcacatcat ggataaagga tacggaggaa gaaacctcgt tgctggaagc
     7861 ctgtaagatg tatcgggtgt tgctgaaccg agttgataca tcaactgccc ctgatattga
     7921 gtggcctgtg aaccctgtca gagagtaatc attgggatta tgccgcagca cgtcgtgcgc
     7981 aagaacgtgc tgcggctgga tgctattttt tccctgaagc ggaaaacatt actacagtac
     8041 ctttaacctt ggttttaaca ttctcgaaat gctctgagag tatatgtgtt aagccttctt
     8101 cggaatcttt tgtgtttgaa aagatgcctt tctgattgta aatgcgcatc agtttttgac
     8161 cgaagctatt gtgcacaacg ccgtcaccaa gaattgtggc tccgtataga gttccatcgt
     8221 cagttaaggc ctgcgccgca ttgcgtatta cacagctttt tgtagatata tttccaggca
     8281 ggcagtgaag aaggtaaaac atggaaatgg aatcaaattg accatgtaac gccgcgggat
     8341 aaggatcaaa aacatcatgg ttaattttat gtttaatttt tgattcccca gcccttgtcg
     8401 atgccgcgtt caggctagct tcgttcaaat ccattaaaga tatcagacta ctctcaggta
     8461 cgtgagtaag gtaaaaccca gttccaacgc caatatccag atggttgtta cctaaatgtt
     8521 ccagaaagtg tggaagaagg tgttcctttg taggacatcc ccatgcaagc cgatttgata
     8581 cacccaaaac ccaccagtca taaagcttta gggtaagtgg tgtgtaaatt ttagccccat
     8641 catctgtgtt ttttttcatt gatttcacca
//
LOCUS       DAWDRC010000092         8627 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4013_length_8627_cov_2.361759, whole genome shotgun sequence.
ACCESSION   DAWDRC010000092 DAWDRC010000000
VERSION     DAWDRC010000092.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4013_length_8627_cov_2.361759"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5618..5627
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6105..6114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2104 a         2254 c         2229 g         2020 t
ORIGIN      
        1 aggaattaac ataaatgact gaaaaacaaa gcctacgtgc ttcgcgcgca actttgcccg
       61 cgcttcttcg tccatattat gtagcggttg tcccaccaga ctcacttcgc cactgctgcc
      121 gtcatccagc ccggcgagga tcgccagcaa ggttgactta cccgatcccg actcgcccac
      181 cagtgcgatg gtctcgccac gtttgacaac cagctcaact ccggtgagga tggagagttc
      241 atgctccccc tgaccgacgg acttcttaag atgatgaact tcaacaatgt tttccgctgg
      301 catttgccct tcctgttcct ggtcctgtta accttccgtg ccgccgcagc agacacgtta
      361 ttgattctgg gtgatagcct gagcgccggg tatcgaatgt ctgccagcgc ggcctggcct
      421 gccttgttga atgataagtg gcagagtaaa acgtcggtag tcaatgccag catcagcggc
      481 gacacctcgc aacaagggct ggcgcgcctt ccggctctgc tgaaacagca tcagccgcgc
      541 tgggtgctgg ttgaactggg cggcaatgac ggtttgcgtg gttttcagcc acagcaaacc
      601 gagcaaacgc tgcgccagat tttgcaggat gtcaaagccg ccaacgctga accattgtta
      661 atgcaaatac gtctgcctgc aaactatggt cgccgttata atgaagcctt tagcgccatt
      721 taccccaaac tcgccaaaga gtttgatgtt ccgctgctgc ccttttttat ggaagaggtc
      781 tacctcaagc cacaatggat gcaggatgac ggtattcatc ccaaccgcga cgcccagccg
      841 tttattgccg actggatggc gaagcagttg cagcctttag taaatcatga ctcataaagc
      901 aacggagatc ctgacaggta aagttatgca aaaatcggtc ttaattaccg gatgttccag
      961 tggtattggc ctggaaagcg cgctcgaatt aaaacgccag ggttttcatg tgctggcagg
     1021 ttgccggaaa ccggatgatg ttgagcgcat gaacagcatg ggatttaccg gcgtgttgat
     1081 cgatctggat tcgccagaaa gtgttgatcg cgcagccgac gaagtgatcg ccctgaccga
     1141 taattgtctg tatgggatct ttaacaatgc cggattcggc atgtatggcc ccctttccac
     1201 catcagccgt gcgcagatgg aacagcagtt ttccgccaac tttttcggcg cacaccagct
     1261 caccatgcgc ctgttacccg cgatgttgcc gcacggtgaa gggcgtattg tgatgacctc
     1321 atcggtgatg ggattaatct ccacaccggg tcgtggcgct tacgcggcca gtaaatatgc
     1381 gctggaggcg tggtcagacg cactgcgcat ggagctgcgc cacagcggaa ttaaagtcag
     1441 cctgatcgaa cctggtccca ttcgtactcg cttcaccgac aacgtcaatc agacgcaaag
     1501 tgataaacca gtcgaaaatc ctggcatcgc cgcccgcttt acgttgggac cggaagcggt
     1561 ggtcgacaaa gtgcgccatg cttttattag cgagaagccg aagatgcgct atccggtgac
     1621 gctggtgacc tgggcggtaa tggtgcttaa gcgcctgctg ccggggcgcg tgatggacaa
     1681 aatattgcag gggtgagttg aagcgcgcgc ttaagccccc atgtcaaaga aaatatcgac
     1741 aacagagagc gactccatgt ccgtagaaaa tattgtcaac attaacgaat ctaacctgca
     1801 acaggttctt gaacagtcga tgaccactcc ggtgctgttc tatttttggt ctgaacgtag
     1861 ccagcactgt ttgcagttaa ccccaattct ggaaagcctc gcggcgcagt acaacgggca
     1921 atttattctg gcgaagctgg actgcgacgc ggagcagatg attgccgccc agtttggtct
     1981 gcgcgcgatt ccgaccgtat atctgttcca gaacgggcaa ccagtagacg gcttccaggg
     2041 gccgcaaccg gaagaggcga tccgcgccct gctggataaa gtgctgccgc gtgaagaaga
     2101 gctgaaagcg cagcaggcga tgcaactgat gcaggaaggc aattacaccg acgctctgcc
     2161 attgctgaaa gacgcctggc agttgtcgaa tcagaacggg gaaatcggcc tgttgttggc
     2221 agaaacgctg attgcgctga accgttctga agatgcagaa gccgtgctga aaaccattcc
     2281 gttgcaggat caggacaccc gctatcaggg gctggtggcg caaatcgaac tgctgaagca
     2341 ggcggctgat acgccggaaa ttcaacagtt gcaacagcag gtggcggaga atccagaaga
     2401 tgccgcactg gcgacgcaac tggcactgca actgcaccag gttgggcgca atgaagaggc
     2461 gctggagtta ctgtttgggc atctgcgtaa agatctcacc gccgcagatg gtcagacacg
     2521 taaaacattc caggagatcc tcgctgcgct gggtacgggt gatgcactgg cgtcgaagta
     2581 tcgccgccag ctgtatgcgt tgttgtattg atgttttgtt gatcggcaca tgaaccacaa
     2641 cgccggatgc gatgctgcgc atccggcatt gcatcatttc ttcttcaatt gcgtcaccac
     2701 caactggtgg cgcgaattgt aaaacttacg ataggttaaa tagcaggcaa taatggtaga
     2761 caggctggca gttgagagca gcataaaggt caccataatc tgatatttaa tcgccttcac
     2821 cggatcaatc ccggcaaata tcagcccgga catcattcca ggtaaactca ctaagcctac
     2881 cgtttttgct gaatcgaccg tcggaattaa agccgcgcga atactgtcgc gaatcaatat
     2941 cgctgaagcc tgtttcggcg tcgcgccaag actcagtttc tcctgaatct gttgctgttc
     3001 gctaatgacc cgttgcccta aattgttgta acacaacccc accgctacca tggcgttgcc
     3061 ggctatcatc ccggcgatag ggatcacctg catcgggata aattcaatcg acccagagag
     3121 gatcagtacc gccagggtaa ttcccgctcc gaccgtaatg gaaataaacg atgagataaa
     3181 agctttagca atatatttac tgcgtttttg cgcgttccac gccgcattaa agcagataaa
     3241 taacaccatc aataatgtca ggctggcatc atccacgctg aaaatatact tcagcacata
     3301 gccgacaata atcagctgga ttatcgcgcg cccgacgctc cagagaatat ctttctccag
     3361 cgccagtttt tctttatggc taattaagat tgccaccacc accagcatta atgccagtgc
     3421 taatgattcg ttagtaatat tatgcgagtt catagcgtgc ttcctgcatt tctccagcat
     3481 gcggttgcag tgtaatcact ttatccgcat gattaatttc gtctttatcg tgtgtcaccc
     3541 acagcacagc aatattttgc tcgcgtacat aacggtggat catctcatta acgttatgtt
     3601 tattactttc atccagcgca ctggtgattt cgtcgagcaa taaaaccttc ggcataaatt
     3661 gcaggttacg aatcaatgag atgcgttgtt tttcaccacc agatagctcg gcgatattct
     3721 tcgtcaaaat gctgtccggt aaagcgaagc gttcaagaaa atcgagaaaa atggccgggt
     3781 caggctgctg gttacggatc tgccagggaa aaatcaggtt atcgtatacc gtatcgccaa
     3841 acagcgtcgg cgtctgggcg cagtaagaga cttgctgacg gtagatttct ggctttagtg
     3901 tgctgacatc ctcaccttca aacagtaacg ttccgctggt tgggctgatc aatgaagcaa
     3961 ctatttttag cagtgtactt ttgccacaac cagaaggacc ggtaattaac ttaaattcgc
     4021 cagcacgcag cgaaaaattg atgttattaa gaatcttcgt atcacccgcc agatatccta
     4081 cgttttgtag ctgaagcaaa ggactatttt cctgcatcgc tgttcccttt ttctgatttt
     4141 tactaaaaac agtttatcct tcgcaggaat aagggggaac tctctttcag taatcaggta
     4201 taatttgttt aatcaagcag caattttgct gattatggac ggtacaacag gaggtttttc
     4261 cgatgcttat ctttatcccg attctcattt ttgtcgcgct ggtcattgtc ggcgcgggcg
     4321 tcaaaatcgt gccgcagggc tatcaatgga cggtagaacg ctttggtcgc tataccaaaa
     4381 cgttacagcc aggtctaagc ctggtggtgc cgtttatgga tcgcattggt cgcaagatca
     4441 atatgatgga gcaagtgctc gacatccctt cccaggaagt tatctcgaaa gataacgcca
     4501 acgttaccat cgacgctgtg tgctttattc aggtgattga cgcgccgcgc gcggcttatg
     4561 aagtcagcaa tctggagctg gcgatcatca acctgaccat gactaacatc cgtaccgtgc
     4621 tgggttcaat ggaacttgat gaaatgctct ctcagcgcga cagcattaac tcgcgtctgc
     4681 tgcatattgt cgatgaggcc accaacccgt gggggattaa agtcacccgt atcgaaattc
     4741 gtgatgtgcg cccgcccgca gagcttatct cttcaatgaa cgcgcagatg aaagcggaac
     4801 gtaccaaacg cgcttacatc cttgaagcgg aagggatccg tcaggcggaa atcctcaaag
     4861 ccgaaggtga aaaacagtca caaatcctga aagcggaagg cgaacgtcag tcggcgtttt
     4921 tacaggctga agcgcgtgaa cgttccgctg aagcagaagc ccgcgccacc aaaatggtgt
     4981 ctgaagccat cgcctccggt gatattcagg cggtgaacta cttcgtagca cagaaataca
     5041 ccgaagcgtt acagcagatc ggttcctcca gtaacagcaa agtagtgatg atgccattag
     5101 aggccagcag cctgatgggg tcgattgccg ggattgccga gctggtgaaa gacagcgcca
     5161 acaaacggac tcagccatga tggagttaat ggtcgttcat ccacatattt tctggctcag
     5221 tctcggcggg ttgctgctgg cagccgagat gttgggcggc aatggttatc tgttgtggag
     5281 cggcgtggca gcggtgatta ccggcctggt ggtctggctg gtgccgctgg gttgggagtg
     5341 gcaaggggtg atgtttgcca ttctgacact gctcgccgcc tggttgtggt ggaaatggct
     5401 gtcgcggcgg gtgcgcgaac aaaagcacag cgacagtcat ttaaaccagc gcgggcagca
     5461 gctgattggc cgacgttttg tgctggaatc tccactggtc aacgggcgcg gtcatatgcg
     5521 cgtcggtgac agttcatggc ctgtcagcgc cagcgaggat ctcggcgctg gtacgcatgt
     5581 tgaggtgatt gcgatagaag gaataacgct gattatcnnn nnnnnnngcg gtcatcgcct
     5641 gatgcgtctt atcacacctg gaaatctgta ccaaacccca tgccggataa ggcgtttacg
     5701 ccgcatccgg catatagccc cctacatagt gttaattgaa tgttcggcta aggagtagcc
     5761 atgttttccc cctttcgaaa acctgaagta cgtctgctga aaaatactgc tgcgggcgtc
     5821 attttttcgg tgaagtccgg gaaaggattt ctgcgtcaat gtgtgattta cgatccgcac
     5881 tcgcacggtc ttatccattc gctctgctgg caggatgtcc cgttaatacg tttctggtcc
     5941 gaggctgggt gccctacctg tgcggaattt gtctacagcg gttttgcgga ggatgaacag
     6001 ggagccgccc ggtttttgtc tgctctcgaa aactggaatc gcccgtggtc tggcataacg
     6061 gacgcttttg ccgcgctgac accgctgttc tcatgcatgg ctgannnnnn nnnngctgat
     6121 ggttattatt tgctggaaga tcgtgaactt tacccgacgg atggcaacgg gcatttcttt
     6181 tgggcggcaa cggaccatag ttccagtaat ccggcaaccg ttgccgtctg ggatcctgaa
     6241 tattgctctt tcagtgacac cgcgccctgc tttcttctac ccggccagcc gccatcacat
     6301 tttaatcctg agcgtgcgac gttttatcgc gataaaccgg acgcccgcgc tctggcctgg
     6361 tatctccctg attcctatct ctgcgttctg ctcgacggac accataaggc tactgccgcc
     6421 gcgctggaag ggcgaccgct caaaacactg gtgctcagca cggcaaccca ttttaatgat
     6481 gagcaacaaa ccctggtatt tcctggcggg gaatgtctgc acaaaacaga gctgcaatac
     6541 cacataccaa aactcaccgc gtgcaaaaca ttaccgccca gcgcatggga gagtttcggc
     6601 cctgataagc acatcagccc atccgaaacc tggtcagaag agttaaagca gagcgtcagt
     6661 cggtacccct cgctggatca agcctggcaa attgttgagg ccgggaattt gagcgaaact
     6721 cgtattaaga gcatgatcca acaaggtttg ggggaggatg aaaaggctga tattattttg
     6781 caggcgctgt tttccacaca ctcgccgctt tttattgact ttgcccgatt cgttatcagc
     6841 catccggcat acgccattta tcgccccctc accttccggt tgatggcgca aaaccgaacg
     6901 ccgcaagcag atgctttttt tctggatttt gccatcaatg atgacgggga acgtccggag
     6961 ttgacgaaaa taatggacga ctattttcgc aagccttaat caccccgccc gatgatgaca
     7021 gcagccggaa agattttcga taatcgggca gtcggcgctg tcatcaccgg ggcaggcatt
     7081 cgctagcgcc agcagctggt tacgcatgga ttgcagttct tcgatatgct gttcgatctc
     7141 cgctactttc tccagcgtac gacgtttgac gtcggcgctg tgacgctgcg gatcgttaaa
     7201 cagattcacc agttcgccgc tctcctccag gttaaagccc acctggcgtg cctggcggag
     7261 taaggtcagt tcgttgagat gctgctgcgt gtaggtgcga taaccgtttt cgctgcgcat
     7321 cggcggcgtc accagcccct tctcttcata gaagcgaatg gctttgctgg tcaggccggt
     7381 aatttttgct acatcgctaa tgttcatcgt tcgcgcaacg cctcctgtgc tttgttaaag
     7441 ggtttcaaca gatattcaag cacgtttttt tgccagtgcg aatatcgacc gccaccacaa
     7501 taatacagga ggtactgcgc acagagagat gttatacagt tttattgcgc ttcatgacca
     7561 ttgccacaat aaggctgagt aagagtgcgc caataatcca tatcagcgca cggctgccct
     7621 gggaccagag actgtagata aagccaacga tcaccaacaa cataaccaac gtaccaacga
     7681 taagaatcgg caacgaggcg tgaatatcat gacgcaggcg gatcgccacc acaaacaccg
     7741 ccagatagca gatcaaaaag gtggcgctgg caacgctggc gagtgagcct aaattcagcg
     7801 ccgccgtcat cagcataatc aacaccacga cgatgatgtt gccccaggta ctctgccgcc
     7861 acagaggttt attcattagc ttcggcagtt cgcgttcgct gcccatgttg tccataatgt
     7921 taaacacggc gaacaggttt gcgttgatgg ctgaagccgt cgccagtaaa gcgccgatga
     7981 cgacgatcac ataccccaca tgcccgagga gcggagaagc agcctgcgct acggcggtat
     8041 cggcatattt ttctaactct aatgccgata catcgctaag caaaaccagc gccagcgaga
     8101 tataaagcaa cgtggtaacg ccaatcgcca ccagaaacgc ccgtggcatt atgacctccg
     8161 gatctttcac tttatccgcc gcgttcgcca tcatgccaaa gcccgcatag gcaaggaagg
     8221 ttatcccgat acaggagaaa aacgccccgg agctgggggg cgcagaaacg gaaatatgcg
     8281 ccggttgcag cgaccagaca ccggcaataa tcagcaataa caggatcatc attttaatgc
     8341 cgacgaggat cacttccagc cgccctaccg catggttgct taaggagttg aaaagcgtca
     8401 tcaccgcaat gatccccaac gcgtagagca aaataaggtg ctcctcctgg ctgccttcat
     8461 gcaaaaactg cacggcataa gcgccaaaag cacgagcgac catggcgata ctcaccgcca
     8521 gcgtcaacag gtacaataac gagagtgcca gcgaaaaaac gccgttgcct aatccgcgac
     8581 gaaagaagtc gataataccg ccattgctgg gatagctcgc ccccaga
//
LOCUS       DAWDRC010000093         8580 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4038_length_8580_cov_2.559296, whole genome shotgun sequence.
ACCESSION   DAWDRC010000093 DAWDRC010000000
VERSION     DAWDRC010000093.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8580)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4038_length_8580_cov_2.559296"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2888..2897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3833..3842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4779..4788
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6226..6235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2059 a         2261 c         2311 g         1909 t
ORIGIN      
        1 ttatcgcggt ccatcgcgta gtaacgacca atgatggacg ctacgcggcc tttacccagc
       61 gcggcaaatt tctcttcgaa ttttttcagc gaggattcag cactgcgcgg cggagtgtcg
      121 cgaccgtcaa ggaatgcgtg taggtagatt ttttctgcgc cgcgttcagc tgccagttct
      181 accatcgcca tgatgtgatc ttcgtggctg tgtacaccac ctgcggagag cagacccata
      241 atgtgtactg ctttgcctgc gtttttcgct ttatctactg caccagtcaa caccggatta
      301 gcaaagaaag cgcgatcttt gatttcaacg tccagacgag tcaggtcctg atacacgatg
      361 cggccggcac ccaggttaac gtggcctact tcggagttgc ccatctgacg gtcaggcaga
      421 ccgacttcca gaccggaagc gtcgatcagg gtatgcggac gattggccca cagtgcatcc
      481 attaccgggg ttttagcact aaaaatggcg ttatcctgct gttcttcgcg atagccatag
      541 ccatccagaa tcaccagtac cataggtttt ttagaaaccg acattgcgac aacctcatac
      601 tcaagagtca aaatttgcgt aattttacta cagctgaatc gataaaatag cctctgaaga
      661 tcaaaatata cgaccgctcg cggcaagcag atgccagatt tacgcttttt tttcgtagcg
      721 ccccgcaaaa ttgcattcca gttaacgcgc tggctgtatt tgccgcaacg cgcaggtata
      781 ctcctttcct ggttttttta atcactacgt cgggagttgt taccccccat gcaagaaatt
      841 atgcaatttg ttggccgtca tcccatactg agtatcgcct ggatcgcgtt actggtggcg
      901 gttcttgtga ctacgtttaa gagcctgacc tcgaaagtga aggtgattac tcgtggtgaa
      961 gctacgcgtc tgatcaacaa agaagacgct gtggttgtgg atttacgtca gcgtgatgac
     1021 ttccgtaaag gtcatatcgc aggttctatc aacctgttgc cgagcgaaat caaagccaac
     1081 aatgttggtg agcttgagaa gcacaaagac aaaccggtta tcgtggtaga tggttctggc
     1141 atgcagtgcc aggagcctgc aaacgcgctg acgaaagctg gttttgcgca agtattcgta
     1201 ctgaaagaag gcgtcgctgg ctgggctggc gaaaacttgc ctttggtgcg cggcaaataa
     1261 tttacctgtc gtctttcatg atgcaggccg ttagctgcaa ttaggccccc agcgacttat
     1321 tgatataagc ttctggggac actccatttt gctgccgccc tgcaacatga aatttcaggg
     1381 taaaaaacag gagctaattt atggccaatg ttgaaatcta taccaaagaa acctgcccgt
     1441 attgccatcg tgcaaaagca ctgctgagca gcaagggcgt gagttttcag gagttgccga
     1501 tcgatggcaa tgccgctaag cgtgaagaga tgatcaaacg cagtggtcgc accacggttc
     1561 cacagatttt tattgacgca cagcacattg gtggctgtga tgacttgtat gcattggatg
     1621 cacgtggtgg actggatccc ctgctgaaat aacgtgtgaa cgttggcatt acattgcgca
     1681 gtatttaagg acaacactta agggttttct acacatgtca gaacaaaaca acaccgaaat
     1741 gactttccag atccaacgta tttataccaa ggatatttct ttcgaagcgc cgaacgcgcc
     1801 gcacgttttc cagaaagatt ggcaaccaga agttaaactt gatctggata cggcatctac
     1861 tcaactggca gatgacgttt acgaagtggt actgcgtgtt accgtaacgg cctccctggg
     1921 cgaagaaacc gcgttcctgt gtgaagttca gcagggcggt attttctcca tcgcgggtat
     1981 cgaaggcacc cagatggcac attgcctggg cgcatactgc ccgaacattc tgttcccgta
     2041 tgctcgtgag tgcatcacca gcatggtatc ccgcggtaca ttcccgcaac tgaaccttgc
     2101 gccggttaac ttcgatgcgc tgttcatgaa ctatttgcag cagcaggctg gcgaaggtac
     2161 tgaagaacat caggatgcct gatgaaccaa cgtaatgctt caatgactgt gatcggtgcc
     2221 ggctcgtacg gcaccgctct tgccatcacc ctggcaagaa atggccacga ggttgtcctc
     2281 tggggccatg accctgaaca tatcgcaacg cttgaacgcg accgctgtaa cgccgcgttt
     2341 ctccccgatg tgccttttcc cgatacgctc catcttgaaa gcgatctcgc cactgcgctg
     2401 gcagccagcc gtaatattct cgtcgtcgta cccagccatg tctttggtga agtgctgcgc
     2461 cagattaaac cgctgatgcg tactgatgcg cgtctggtgt gggcgaccaa agggctggaa
     2521 gcagaaaccg gacgtctgtt acaggacgtg gcgcgtgagg ccttaggcga tcaaattccg
     2581 ctggcggtta tctctggccc aacgtttgcg aaagaactgg cggcaggttt accgacggcc
     2641 atttcgctgg cctcgacgga ccagaccttt gccgatgacc tccagcaatt gctgcactgt
     2701 ggcaaaagtt tccgcgttta cagcaacccg gatttcatcg gtgtgcagct tggcggcgcg
     2761 gtgaaaaacg tcattgccat tggcgcgggg atgtccgacg gtatcggttt tggcgcgaat
     2821 gcgcgtacgg cgctgatcac ccgtgggctg gctgagatgt cgcgtcttgg cgcggcgctg
     2881 ggtgccgnnn nnnnnnnctt gggggggctg ggcgatctgg tgcggacctg taccgacaac
     2941 cagtcgcgta accgccgttt tggcatgatg ctcggtcagg gcatggatgt acaaagcgcg
     3001 caggaaaaga taggtcaggt ggtggaaggc taccgcaata cgaaagaagt ccgcgaactg
     3061 gcgcatcgct tcggcgttga aatgccaata accgaggaaa tttatcaagt attatattgc
     3121 ggaaaaaacg cgcgcgaggc agcattgact ttactaggtc gtgcacgcaa ggacgagcgc
     3181 agcagccact aaacccaggg aacctttgtt accactatga cccggcccgc gcagaacggg
     3241 tcggtcatta tcttatcgtg tggagtaagc aatgtcgtgt gaagaactgg aaattgtctg
     3301 gaacaatatt aaagctgaag ccagaacgct ggcggactgt gagccaatgc tggccagttt
     3361 ttaccacgcg acgctactca agcacgaaaa ccttggcagt gcactgagct acatgctggc
     3421 gaacaagctg tcatcgccca ttatgcctgc tattgctatc cgtgaagtgg tcgaagaagc
     3481 ctacgccgcc gacccggaaa tgatcgcctc tgcggcctgt gatattcagg cagtgcgtac
     3541 ccgtgacccg gcggtggata aatactcaac cccgttgcta tatctgaagg gttttcatgc
     3601 tttgcaggcg tatcgcatcg gtcactggtt gtggaatcag ggccgtcgcg cgctggctat
     3661 ttttctacaa aaccaggtct ctgtaacgtt ccaggttgat atacacccgg cagcaaaaat
     3721 tggtcgcggt atcatgctcg accacgcgac aggcatcgtc gttggtgaaa cggcggtgat
     3781 tgaaaacgac gtatcgattc tgcaatctgt gacgcttggc ggtacgggta aannnnnnnn
     3841 nnatctggtg gcgaccgtca cccgaaaatt cgtgaaggtg tgatgattgg cgcgggcgcg
     3901 aaaattctcg gtaatattga agttgggcgc ggcgcgaaga ttggcgcagg ttccgtggtg
     3961 ctgcaaccgg tgccgccgca caccactgcc gctggcgttc cggcacgcat tgtcggcaag
     4021 ccggatagcg ataaaccgtc aatggatatg gatcagcatt tcaacggcat taaccatacg
     4081 tttgagtatg gtgatgggat ctgatgtcct gtgatcgtgc cggatgcggc gtgaacgcct
     4141 tatccggcct accgcatctt aatctctcaa caccgctccc ggatacccca gctggcgcca
     4201 ggcttcatac accacgaccg acaccgcatt ggacaggttc atgctgcgac tgtccggcac
     4261 cattggaata cgaatttttt gttcagcggg cagggcatca agaatgctcg ctggcaggcc
     4321 gcgtgtttcc gggccgaaca tcagataatc gccatcctga tagcttacgg cgctgtgagc
     4381 aggcgtacct ttcgtggtga gggcaaacag gcgctgggga ttttctgctt caaggaacgc
     4441 gcgatagtca tgatgacgcg taacggcggt aaactcgtga tagtccagcc ccgcgcggcg
     4501 caggcgctta tcgtcccagg caaatcccat cggttcgatg atatgcagac gaaagccggt
     4561 attagcgcaa agacggatga tgttgccagt atttggcgga atttctggtt cgtaaagtac
     4621 gatgtttagc atactgcccc cttagtgcgg gggcaggata gcagatattc gtctaaccct
     4681 taagctgcat tccctttcgc cattggagcc agtgccgcag gcaactcttt acccagcccc
     4741 tgcaccagcg aatcttgcgt aatttcgcta atcgatttnn nnnnnnnncc tggcccgctg
     4801 ttgccagtgc atacaggaaa gcacgaccca gcagtacggt gtcggcaccg agcgcgatca
     4861 tacgcacgac atcaagcccg ttacgtattc cgctatccgc cagaatggcg atatcacctt
     4921 tcacggcatc tgcaatagca ggcaacgcac gggcagaaga cagcacaccg tccagctggc
     4981 ggccaccgtg gttagaaacc acaattccat cagcaccaaa acgtactgca tcgcgcgcat
     5041 cttccggatc gaggatccct ttgatcacca tcgggccatc ccagaaatcg cggatccact
     5101 caaggtcttt ccatgagatg gacggatcga agttattccc cagccagccg atgtaatctt
     5161 ccagtccggt cggtttgccg agataagctg agatattacc taaatcatgc ggacgaccgt
     5221 tcaggcccac atcccacgcc cattgcggat gcgtcaccgc ctgcaaatag cggcgcattg
     5281 ccgcgttcgg gccgctcatc ccagaatgcg catcacgata acgcgctccc ggtgttggca
     5341 tatccacggt gaataccagc gtcgaacaac ccgctgcttt tgctcgctcc agtgcgttac
     5401 gcataaagcc gcgatcgcgc agcacataaa gctggaacca catcggacgt ttgatagccg
     5461 gagccacttc ttcaatcggg caaacggaaa ccgtcgagag agtaaacgga atgccatgcg
     5521 catccgcggc tttggcagcc tgaacttcgc cgcgtcgcgc atacatgcca cacaaaccta
     5581 ccggacctag cgccaccggc atcgacagtt tctcattaaa cagcgtcgtt tccaggctta
     5641 agtcagacat gtttttcaga atacgctggc gcagcgccac ttctgacaaa tcttccacgt
     5701 tgcggcgcag cgtgtattca gaatatgccc ccccatccat atagtggaac aggaacggcg
     5761 gcagaatgcg ttgcgctgcg gcgcgataat cgctggctgc ggaaataatc atgcgttttt
     5821 ctccctcgaa tgctcattat ggtcaccagg caggcgggta atgcgtgcat ggcgagcctg
     5881 atcttcatcg aatcgtttca tggtggtgtg aacaaagcta aggtgcgcca tcattgcttt
     5941 acgcgccccg tcagcatcac cggcaaaaat ggcgtcaatg accgcctgat gttgttcggt
     6001 cagctgtgaa aaaactgggg gtaccaggta catccgctgg cggctatgct tcacagagga
     6061 ttgcaggaca tcgaagaaac cgcgcatggt ttgcagcagc acgatgttat gtgaggcttc
     6121 ggcaatcgcc agatgaaaac gaacgtccgc ttgtgaggcg agatccgggt cttcgctgag
     6181 cgttgcttca aaacaaagct gaatcttttc tttttcgcca ggcgtnnnnn nnnnngtgct
     6241 ggcttcaatg gcgtagcggg cttccagaat atcgaaactg taatccggat catcggccat
     6301 cagcgttttc agcggctgga cgatgttttg ctccgaccat gtgtcatgac gccagcgaat
     6361 aaacgtcccg ccgccacgtc gactgagcag cacaccttcg cttaccagtt tcgccagcgc
     6421 ctcgcgcagt gaatttctcg acacgccgag ttgcatcgcc agttgacgtt cagcgggcaa
     6481 cttcatgccc gcttccaggt ttttttcatc aatcagcgcc cgcacacgat cggcaacctc
     6541 gtctgacagg cgtctgggta aaacaatcat taaggaatca tccacgttaa gacataggcc
     6601 tgaagcgtgg tgatcacgcc caccatacag gtgaagatca ggctgtgttt gacagtaaag
     6661 cggaacaaat cagactcttt gcccaccagg cctaccgccg cacaggcgat agcgatagat
     6721 tgcggggaga tcatcttacc ggtgacacca ccggtggtgt tggctgcaac cagcaacagg
     6781 tcagaaacgc caatttgttg tgcggcggta gcctgcaaag cggcaaacaa agcattagat
     6841 gacgtatccg acccggtcag gaatacccca agccagccga ggaacggcga gaagaaggtg
     6901 aatgcatgac cggtgtgcgc cagtgccagc gccagtgttg atgacaaacc ggaatagttc
     6961 gagataaagg cgaacgccag caccatgccg atggagtaga taggcagagc cagttctttc
     7021 agtgtgctgc cgaaggtgct gatagcgtca gatggtttca tcttcagcca gacaatcgag
     7081 agcagggcag caaacagaat ggcggtaccg gtggcagaga accagtcgaa cttaaacacg
     7141 gcggcatacg ctgtcgcttc gctgaccact ggcggcatac gggcaaccag tttatcgagg
     7201 tacggcaccg gaatgttgat cacccactca tacagcacac cgcctgaagt gaacagggct
     7261 ttaaacggcg ggatactcca cagcgttacg gtggcggtca ggaacaggaa cggtgtccag
     7321 gcgcgcagca cctgacctgc cgtgtaacct gtgtgagcca gcgtcatatc aacctgtgac
     7381 gcccctaagt caccgaagcg gaatacgcgc actggttgcc agcgtttgag gaacagtgtc
     7441 agacagagca gcgatacccg cgaagagata atgtctggca gttccggccc aataaagtta
     7501 gagctgaggt attgagcgat ggcaaacgac ccgcccgcaa ccacgaccgc aggccaggtt
     7561 tctttgatac cgcgccagcc gtccataatc gccatgatcc agaacagcac gataatggtc
     7621 ataaacggca gctgacgccc caccatctga ccaatctcaa agctgtcgat gcctgttacc
     7681 tggccggcaa ccaggattgg aatgcccatc gcaccaaatg ccactggcgc ggtgttaaca
     7741 atcaggcaca gcccggcggc gtacagtggt ttaaagccca gaccgaccag caatgcggcg
     7801 gtaattgcta ccggtgcgcc aaagcctgcg gctccttcaa ggaaagcgcc gaaacagaaa
     7861 ccgacgatca gcatttgcag gcgctggtca ggggttatcg aaagaataga cgagcgaatg
     7921 atgtcaaact gcccggtttt caccgagatc ttatagacga acaccgctgc aataatgatc
     7981 cacgcgatgg gccacaaccc gtagaagaag ccataaacca ccgaggccag cgcgttagcg
     8041 accggcattt tatagaacag caaagccacg gcaagggcga ttgccaccgt ccatgaggcg
     8101 gcgacgtatc ctttcagttt gagcttaatc agcgcaaaga agaaaaacag gatgggaagc
     8161 gatgctatca ggctggaaag ccagatattc ccggcgggat cgtagttttg ttgccagaga
     8221 ttcattgcag gtctcctgga gtccacgcac atcgtgtaat gagtctgttg cccatctcct
     8281 tgtcacacgt tgtgtaaaag tggtactgcc aattgtgttg tgtaggggta atgacaatga
     8341 atggttaacc aaatgttatg tgttggcaac attgatgtga gttttaattg gggaattgtg
     8401 aaccagaggt cagataagcg aagaattggt agggccaatt cttgtggtgg gagatgtctg
     8461 gtaaagaaaa gggcaattcc catcagagaa ttgccctcag attgttgatc tggtgcgcct
     8521 tatcttgccg gatgcggcgt gatgaactga accccaaaac ctggacagtt tcagttaacc
//
LOCUS       DAWDRC010000094         8552 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4055_length_8552_cov_2.056373, whole genome shotgun sequence.
ACCESSION   DAWDRC010000094 DAWDRC010000000
VERSION     DAWDRC010000094.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4055_length_8552_cov_2.056373"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3020..3029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4997..5006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6791..6800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2096 a         2176 c         2260 g         1990 t
ORIGIN      
        1 gatgccgcac tgacccgcta caatgtcaga ttactgccgt aaagaagttt gaggaagcaa
       61 gacaatgagc accactatcg aaaaaatcca acgccagatt gctgaaaacc cgatcctgct
      121 gtacatgaaa gggtcaccga aactgccgag ctgcggtttc tctgcccagg cagtccaggc
      181 gcttgccgca tgtggcgaac gttttgccta tgttgatatt ctgcagaatc cggacattcg
      241 tgcggaactg ccgaaatatg cgaactggcc gaccttcccg caaactgtgg gttgacggcg
      301 agctggtggg cggttgtgat atcgtgatcg aaatgtatca gcgtggtgaa ctgcagcagc
      361 tgatcaaaga aactgccgct aaatacaagt ctgaagagcc ggacgcggaa taatccgtcc
      421 tgcaatactg acggatgcga acgggcatcc gtcagtcctt tctctatccc caatccagcc
      481 cttgtggcga acttgaaaat cccgcctcac gtaaccgcgt aaacatcggc gtttgccgga
      541 ccggtaggtc attcacttct gttagcgtaa agcgcagccg tggttcgcga ctggacagct
      601 gtcggctgtt taaaggcgcg aagaaaccag tcgcgggtcg ccggtgaaaa cacgtccggc
      661 aggggcgatg aagggtttgg attatttgcc attaacgcta ttccccgtga ataacatgca
      721 tgatgcgtag aatagcctca ctatagatgc ctgattcatt gcatgtcatc aggcatcgac
      781 tcgattacac ctcttcggcg gcaggcagcg gccagcctcc cagacgtttc cagcggttga
      841 cgatttcaca aaacagcaca gcagtgcgtt cggtgtcgta cagcgcggag tgcgcctggg
      901 tgctgtcgaa gtccatgcca gcggtctggc aagcctttga caatacggtt tgtccgagtg
      961 ccagcccggc cagtgcagca gtgtcaaaag tggcgaaagg gtggaacggg ttacgtttca
     1021 gtgaggcgcg ttctgcggcg gccatcataa agctgtgatc gaaattggcg ttgtgcgcca
     1081 ccataatggc gcggttacag ccgctcgctt taataccttt acgtacaact ttaaaaattt
     1141 cgtgcagcgc ctcgtactcg ctgaccgcgc cgcgatcggg atcgttcgga tcaatgccat
     1201 tgaaggcaag ggcctctggt tgcaaatttg cgccgacaaa tggttcgacg tggaaatgta
     1261 atgtggtgtc cggcatcagc cagccttgtt catccatttt cagggtgatg gcggcaatct
     1321 caagcagcgc atcggttttg gcgttaaatc cggctgtttc aacatcgatc acaacaggat
     1381 aaaaaccacg aaaacggtcg cacagaccgg taagttgagc gttatcggac atctgattct
     1441 cttaatacgg ataaattgca gcgcgcatta tgacaaatat tacttgtcga tgcagtaaag
     1501 atgcgggcgc gatgagttca cgcccggcag gagattagtt gcccagaccg cgaccggcgt
     1561 ctttctcttc gattaattca attttgtaac cgtccggatc ttccacaaat gcgataaccg
     1621 tagtaccgcc ttttaccgga cccgcttcac gggttacgtt acccccgttt tgacggattt
     1681 tttcgcacgc ttcagcggcg ttatctacgc taagcgcgat gtgaccataa gcagtgccga
     1741 gttcgtattt atccacgccc cagttgtagg tcagttcaat cactgcttct tcggtttccg
     1801 ggccgtaacc aacaaacgcc agtgagtatt tgtattccgg gttttcgctg gtacgcagca
     1861 gtttcatgcc cagcacgttg gtataaaaat cgatggagcg ttgcagatcg ccaacgcgca
     1921 gcatggtatg aagaagacgc atctttatat cctcaaaatg gctaacagat tcaaaaagtt
     1981 gttttagtat agcggcggct ttgcgccgct cgcaatgttg gattacagcg tcgggtaatc
     2041 agtgtaacct tcagcaccgc caccataaaa actttcggca cgctgtgggt taagctcagc
     2101 tttgcgctgc aagcgggcga ccagatccgg gttcgcaatc cagtcacgac caaatgctac
     2161 cgcatcaatt aaccctttgc cgatcagcgt ttcagctttt tctactgtgt atgcacctgc
     2221 gccgataatc ggaccgtgga aacgggcgcg tactttttcg cgaaaagcat ccgtatatgg
     2281 ttcacatccc gcccaatctg gttctgacat atgaagataa gcaatgccgc gtttacccag
     2341 ttgttcaatc agatacagtg catcggcttc ttcattcggg ccgttatccg tgttctggaa
     2401 agtaccgatt ggcgaaatgc gaatgccaat gcgatcggca ccccattctt caatcccggc
     2461 atcgaccact tccagcacca gacgcgcgcg attttccacg ctgccgccgt actgatcggt
     2521 acgatggttt gaagaagggg aaaggaactg atgcagcaaa taaccgtgag cagagtggag
     2581 ctctaccaga tcaaaaccgg cttcacgcgc gttagcaatg gcctgacgga aatcattgac
     2641 gatacccgga atctctccca gttcaagcgc acgcggcatg gatgtttcaa cacggatcgc
     2701 ctgaccattt tcatcgcgca gagaagtacg tgttcccgcg ctaagtgctg aaggcgctac
     2761 cggtgcctga ccgccaggtt gcaggctggc gtgagaaatg cgtccggtgt gccacagctg
     2821 tacggccata tgaccatttt cagcatgaac gccagcggta atttttttcc atgcggcaat
     2881 ttgttccggg ctatggatgc caggtgcacc tgcgtatcct tttgcctggg cagaaatttg
     2941 cgtggcttcg ctaataatca aaccggcact ggcacgttgg cgatagtatt ccgccatcaa
     3001 cggggtaggg atgtcacccn nnnnnnnnna tactgcgcag acgcgtcagc ggtgccataa
     3061 aaatacgatt tgctgccgtg atcgcgccca ctttcagtgg ggaatacagt ttttcagatg
     3121 acataaaggc tcccggtcgt ctggtgataa aaaatgcctg ctaaacggca ggcgtcgcaa
     3181 taatgttttt tacatgggcc agcgcgtttt ccagtggctc gaaactgcgc gaaattttgg
     3241 cctgcagatt cgcgccaagc cacagtgcgt aaagcacttg tgcctgttgc agcggttcgc
     3301 cacaaaaggt taaacaatgg ttctcacggc cattttccag cgcctgagag agcagggcga
     3361 tcacgccgcg agccccttta tccattgcgc tgcgcatatc ttctgacaga tcgcacactt
     3421 cggcagagag ttttactgtc aggcaaccac tgatggttcc atgttggcaa aactggttca
     3481 gtgtttgctg gtaataagcc agtatgcggt cgcggtagtt accttcgccg gattgcagca
     3541 actcagtcag tcgctggtga tatgcggcgt aatggcgctc aagcatagca acgccaaacg
     3601 cttctttaga acgaaagtag tgatagaagg accctttcgg cacttcagcg gtttttagga
     3661 gttcgcttag ccccatcccg gtgaatccac gttgcaggca aagttgctcg cccgtcgcca
     3721 ggagatgttc gcgagtatca tgttcggtgt gtttgttcat gcgttggagt gtagtagacc
     3781 agtcggtcta gtgcaagcaa agttgctact ttggacatta acagcttcaa taatggtaat
     3841 aatcttatct gaggagggcg aggtggcgga gcaattagag ttctttcccg tccagagccc
     3901 gtgccgggga atttgccagt ctgacgaacg cggattttgt cgcggctgtt ttcgtagccg
     3961 ggatgagcgt tttaactgga ataagatgag cgatggcgaa aaacaagaag tgttgcgcct
     4021 ttgtcggcag cgattaatgc gcaaattacg tgtaaataag tcagcgccat cagacgaacc
     4081 tgagcaacca tcactctttt gagcctaatc ttgcgtatac tcctctcata tctgataaga
     4141 ggaagcaatt atggttcagc gtattactat tgcgccgcaa ggcccggagt tttcccgttt
     4201 tgtgatgggc tactggcgat tgatggactg gaatatgtcc gcccgccagt tggtcagttt
     4261 tattgaagag catctggatc taggcgtgac caccgtggac catgctgata tttatggtgg
     4321 ctatcagtgc gaagcggcgt ttggtgaggc gctgaaactg gcacctcacc tgcgtgaacg
     4381 gatggaaatc gtcagtaaat gcggtatcgc gacgaccgcg tgtgaagaaa acgtcattgg
     4441 ccattacatc actgaccgcg atcacatcat taagagcgcc gaacagtcgc taattaatct
     4501 cgcgaccgat catctggatc tgctgttaat ccaccgacca gacccgttaa tggatgccga
     4561 tgaagtggcg gacgccttta aacatctgca tcagagcggc aaagtgcgtc attttggtgt
     4621 atcgaacttt acacctgcgc aatttgcgct gttgcaatca cgcctgccat ttacccttgc
     4681 cactaatcag gtggaaatat ccccggtgca tcagccgtta ctgctggatg gcacgctcga
     4741 ccaactgcaa caactgcgtg ttcgtccgat ggcgtggtcc tgccttggtg gtggtcgtct
     4801 gtttaatgat gattatttcc agccgctacg tgatgaactg gctgtggtgg cagaggagtt
     4861 aaacgcgggc tcgattgaac aggtagttta cgcctgggta ttacgtttac cgtcgcagcc
     4921 gctgccaatt atcggctcag gtaaaattga gcgcgtacgg gcagcagtcg aagcagaaac
     4981 actgaaaatg acccgtnnnn nnnnnnaaca atggttccgt atccgtaaag cggcactagg
     5041 ttacgacgta ccgtaagcac ttttaggaat agccgccgtt caaaaatgtg tcactggttt
     5101 acacttattc aggaatgcac aatgaacgga ggtcctatga aacgttttag tctggccatt
     5161 ctggcgctgg ttgttgcgac cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc
     5221 gtcacgtcgc aaggggttgg gcagttaatt ggtagcgtca ccattactga aaccgataaa
     5281 ggtctggagt tttcgcccga tctgaaagca ttacctcccg gtgaacatgg cttccatatt
     5341 catgccaaag gaagctgcca gccagccacc aaagatggca aagccagcgc cgcagaatcc
     5401 gcaggcgggc atcttgatcc acaaaatacc ggtaaacatg aagggccaga aggtgccggg
     5461 catttaggcg atctgcctgc actggtagtc aataatgacg gcaaggcgac cgatgccgtc
     5521 atcgcgcctc gtctgaaatc actggatgaa gtcaaagaca aagcgctgat ggtccacgtt
     5581 ggcggcgata atatgtccga tcaacccaaa ccgctgggcg gtggcggtga acgctatgcc
     5641 tgtggtgtaa ttaagtaatt aagaagccag cgtaccttgc ggcggtgctt gctcaagttg
     5701 cgaaagcgag cagtacagcc gccagacaat tgccgccagt tctcgggcgg caggttgatg
     5761 atgacgggca aggctgtcgc aaattcgctg taattcttca agtgtggccg ctagtgattt
     5821 ttgctgaacg ccacgctcac tcatcactcc ccgcaacagt gaaatacagt tatcccgtac
     5881 tcgcgataac ggatcggaac gcgattccca gtcgcgcaat tgccagacaa catgagaaca
     5941 gttcagcaac actacacccc agcgtaataa ccaacggcga gccagcgcat cctggctgtt
     6001 actcaactga ctgacgtgat gataagtgag cgattcaaat tcgctttcac tcagtgttgg
     6061 atggcggctt agttgatcga caaaatccca gcgcagcgcg cgaatatggc ggcggctttt
     6121 acgagcatcc gatcctggac gcagaatggc gaacgctaac cacgccaacg cgacgccaac
     6181 aatttttgcc agattatcgt taagaaaatc agcaaaatca tacaccgggg gattagtgac
     6241 agcgataaac gaacccataa aaacaatcag ttgcccccac agtgcggcaa atttgggcat
     6301 ctgcaattta agaagctgca ttgtcgccag cagtggaaag agaaacagta aaaattgcca
     6361 cagatcgcta atctgcacca tcaggccaaa tttgaccaca aagctgaata gcgaaagtaa
     6421 caccagcgtg cgcatcagca gtgacaacga cttaaacggt gccgcgacgg cggagtagag
     6481 tacgcagcta attgctgcca gcgttaaagc attggcaccg gcatcccatt gcgaagcaat
     6541 actccatgcg ccaatcatca tcaacgtaca aaatgtacgc agcccgctcc acatagcttc
     6601 ggcgttatcg gtatgacgag ccaggccgga tgtccgtggg agtctggcac gatcatctac
     6661 accgctttgc agacgatgta attcctgact actttgcaga taaaggcggc aaaaataacg
     6721 tagtcgctgc cagaaggcga cgtgccggta gtcggcgacg ttggtcgggc gtagcgggga
     6781 gataatacgt nnnnnnnnnn gcgacggtgt aaacatctgt ttgcgaactg gcgagcgccg
     6841 tcagcagctg ttcgagaatt tctcgcgtgg catctggcgg tgagggccag ttgagcaaca
     6901 tacgtcgcag gctggagatg acactggtca tacgtaattg ctggtggagt agcgcattaa
     6961 gacgcgcgtt ttgctggcga aaacgatagt ggctccagaa agcctggata cgcagcaaat
     7021 tcatggtcag tatctgccca atcacccctt catgtgctgc acgaatggca tcggttgttt
     7081 caggccgcca gagtaaactg gcatgttcca gtaatcgggc gtgcatgttt ttcaatgcgg
     7141 ttaaaagggc tgtagcatcg gaactgctcg gcaggatcat catcatcatg ccgccgcaca
     7201 aaataccgac tatcacctcg caaacacgcg cctgagcgat atcccacagc tggctggcct
     7261 cagtaatatt aaccatcgga aaggcgatga tggcagccgt gtagcccgcc agttgaaatg
     7321 catacgcgac gttattcgtg aagtgcgcac aggcccaggt acaaaagcca agccacgccg
     7381 acatgctcaa tagaaaaaac cacggctcat tgagcgtatg tccggcaaga agcagtgccg
     7441 caatggctcc gagcaaactg ccagcgatgc gtccgaggct tttgctgatg acaccgccaa
     7501 cggtgggaaa gctaaccacc gcagccgagg tcatcgccca atagggctca tcaagattca
     7561 aataataggc aaccgtcagc gccagacaca tggcaatggt attgcgtaac gcataacgcc
     7621 attgcgccag cgtggccctg aaccagggca aattgcgcaa ggaccatgac gatgcgttca
     7681 ttattgacct acggcaatgc tacaggtggt gcccgacacc agcgtgacat cgcctgggac
     7741 tttatccagc gtaaagcgga cgggaacgcg ttgggcgagg cgaacccagg ggacgttagg
     7801 tttcacatcc ggaataaggc ttgagtcact ttcaacgctc tgatcataaa tcgcccgacc
     7861 gatactggac gcgtgaccct gtaacgtttt gttatcacta tagagggtaa tttgtgctgg
     7921 tgcaccttcg cggatatggc gcaatttggt ttcttcaaaa taaccaatga cgtaaaacga
     7981 gtggctgtca acaagggcaa acagcggttt cccggtgtca gcgtagtcgc cgatgcgggt
     8041 agtgagatta gtcacccatc cactcaccgg ggcgcgaatt tccgtttgcg ccagttgcca
     8101 ttgtgcctgt tttagcgtcg cctgtgcggc atcaacgctg gcctgcatcg ctttaacatt
     8161 gaggttagcg gtatcgagtt cttcggcaga aataaaattt tgtgagagat gacgaagacg
     8221 attcgcttcg ttattggctt ttgcgaggtc agattgcgct ttcgccagct gagcctgtgc
     8281 gttcagctcg gcgatctgaa agggcgtttt atcgatcgtg agcagaagat cccccgcgtt
     8341 aaccagctgg ttgtctttta tattcagctc aacaatacgt cccgacacct gtggggtgat
     8401 agaaacctgt tctgcgcgta ttttcccgtc gcgtgtccac ggagattgca tgtaaaaatt
     8461 ccacagccac catccggcaa gaaccgcaac taccgctaca atgattgttg agaaatactt
     8521 aattgtttta attgacatag ttaccacgca at
//
LOCUS       DAWDRC010000095         8529 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4069_length_8529_cov_2.485249, whole genome shotgun sequence.
ACCESSION   DAWDRC010000095 DAWDRC010000000
VERSION     DAWDRC010000095.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4069_length_8529_cov_2.485249"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1998 a         2127 c         2251 g         2153 t
ORIGIN      
        1 gtcgccctcc acatttcgcg acgcattttt aaaagttggc ctgcgcgcga tgacctgttt
       61 tgaaactact gaccgtaaca gcggcatcaa agagttgcag gaaggtgtag aagaaaacat
      121 ccgcttcgcc cgtcagattg atgaggcgaa gaaagcagca accgagccgt atctggtgga
      181 agcacatatc ggcgctcacg cgccgtttac cgtaccggat gccggtctgg agatgctgcg
      241 tgaagccgtg aaaagcacag gtcgtggttt gcatattcac gctgcggaag acctttacga
      301 cgtttcctac agtcaccatt ggtacggcaa agacctgctg gcacgactgg cgcaattcga
      361 tctcatcggc agcaaaacgc tggtcgctca tgggctgtac ttgtcgaaag atgacatcgc
      421 cctactcaat cagcgcgatg cgttcctggt gcataacgcc cgttcaaaca tgaacaacca
      481 tgtcggctac aaccatcacc ttagcgacat ccgcaatctg gcgttgggaa cggacggcat
      541 tggttcggac atgtttgaag agatgaaatt tgccttcttt aaacatcgcg atgcgggtgg
      601 tccgctgtgg cctgacagtt ttgccaaaac cctgactaac ggtaacgaac tgatgagccg
      661 caactttggc gcgaaatttg ggcttctgga agccggttac aaagccgatt taaccatttg
      721 cgattacaac tcgccgacgc cgctgctggc agacaatatc gccgggcata tcgctttcgg
      781 tatgggctca ggcagcgttc acagcgtgat ggtcaatggt gtgatggtct atgaagaccg
      841 tcagtttaac ttcgattgcg attccattta tgcgcaagcc agaaaagccg ctgccagtat
      901 gtggcgtcgg atggatgcgc tggcataaat aacagctccc ctcttctttc agagaagagg
      961 ggaaattaag gaaagacgat gattgaacaa tttttcagac ccgactctgt cgaacaagcg
     1021 ctggaactga agcgccgcta ccaggatgaa gccgtctggt tcgccggggg cagcaaactc
     1081 aacgctacac caacccgtac cgataaaaag attgccattt ccttgcagga tctggagctg
     1141 gactggattg actgggataa cggtgcacta cgaattggcg caatgtctcg cttgcagtca
     1201 ctgcgtgatg cgcgatttat tcctgcagca ctgcgtgaag ctctcggttt tgtctactct
     1261 cgccatatcc gtaatcagtc gaccattggt ggtgaaatcg ccgcccggca ggaagagtcg
     1321 gtgctgcttc ccgtcctgct ggcactggat gctgaactgg tttttggcaa cggcgaaacg
     1381 ctgtcaatcg aggactacct ggcctgccca tgcgatcgcc tgttaaccga aattatcatt
     1441 aaagatccgt atcgcacctg tgcgactcgc aaaattagcc gttctcaggc aggtttaacc
     1501 gtcgtgacgg cagccgttgc aataacagac cacgacggta tgcgaattgc gctggatggc
     1561 gtcgccagta aagcactgcg tctgcatgat gttgaaactc aaaatctgga aggcaatgca
     1621 cttgaacagg ctgtcgccaa cgccattttc ccgcaggaag atttgcgggg cagcgtggcc
     1681 tataaacgct atatcacggg agttctggta gccgatctgt atgacgactg ccaacaggct
     1741 ggggaggaag ccgtatgatc atccacttta ctttaaatgg cgcgcctcag gagctaaccg
     1801 ttaatccagg cgaaaacgtg caaaagctgt tgtttaacat gggaatgcac tctgtacgca
     1861 acagtgatga tggttttggg tttgccggtt ctgacgcaat tatctttaac ggtaatatcg
     1921 ttaacgcgtc cttgcttatc gccgcacagt tagagaaggc agatattcgt accgcagaat
     1981 ctctggacaa atggaatgag ttaagtctgg ttcaacaggc catggttgat gttggcgtgg
     2041 tgcagtctgg ttataacgat ccagctgcgg ctctgattat caccgatctt ctcgatcgca
     2101 tcgacgcacc tacccgcgaa gagatcgacg acgcactctc aggtctgttt agccgcgatg
     2161 ctggctggca gcaatactat caggtcattg aactggcggt tgcacgtaaa aataatccgc
     2221 aggccaccat tgatatcgct ccgactttcc gtgacgacct tgaagtcatt ggcaagcatt
     2281 atcctaaaac tgatgccgcg aaaatggtgc aggcgaaacc ctgctatgtt gaagatcgag
     2341 taacggctga cgcctgcgtc attaaaatgt tacgtagccc acacgctcac gcactgatta
     2401 ctcatctgga tgtcagcaaa gctgaagcct taccgggcgt cgttcacgtt attactcacc
     2461 tgaattgccc ggatatttac tataccccgg gtggtcagag cgcaccggaa ccgtcaccgc
     2521 ttgaccgccg aatgttcggc aagaaaatgc gtcacgtcgg cgatcgcgtt gctgcggtag
     2581 tagccgaaag tgaagacatt gcgctcgaag cactgaagct catcgacgtt gaatatgaag
     2641 tgcttaagcc ggtaatgtcg attgacgagg caatggcgga agatgcgcct gtcgtgcacg
     2701 atgaaccggt ggtgtatgtt gctggtgcac cagatactct ggaagatgat aaccgtcatg
     2761 cagcccagcg cggcgagcat atgatcatca acttcccgat cggttctcgc ccacgcaaaa
     2821 atatcgctgc cagtattcat ggtcatattg gcgatatgga caaaggcttt gccgatgccg
     2881 atgtgatcat tgagcgaacc tataactcaa cgcaggcgca gcagtgcccg actgaaacac
     2941 atatctgctt tacccgtatg gacggcgatc gtctggtgat ccacgcctcc acccaggtac
     3001 catggcactt acgccgccag gtcgcgcgcc tcgtgggcat gaaacagcat aaagttcatg
     3061 tcattaaaga gcgagttggc ggcggttttg gttccaaaca ggacatcctg ctggaagaag
     3121 tgtgcgcctg ggcaacctgc gtgaccgggc gtccggtact gttccgctac acccgtgaag
     3181 aagagtttat tgctaacacc tctcgccacg tcgcgaaagt caccgtcaaa ctgggcgcga
     3241 aaaaagatgg tcgcctgacg gcagtgaaga tggatttccg cgccaacact ggcccttacg
     3301 gtaaccactc actcaccgta ccgtgtaacg gaccggcgct gtcgctgccg ttatatccat
     3361 gcgataacgt cgatttccag gtcaccacct actacagcaa catttgccca aatggtgctt
     3421 atcagggtta tggcgcaccg aaaggtaact tcgctatcac catggcatta gcggaactgg
     3481 ctgaacagtt acagatcgac caactggaaa ttatcgaacg taaccgggta cacgaagggc
     3541 aagagctgaa aattctcggt gcgattggtg aaggtaaagc gccgacatcc gttccttccg
     3601 ccgccagctg cgcactggaa gagatcctgc gtcagggtcg cgagatgatc caatggtctt
     3661 caccaaaacc acaaaacggt gactggcaca tcggtcgtgg cgtcgccatt atcatgcaga
     3721 aatcgggtat cccggatatc gatcaggcta actgcatgat caaactggaa tcggatggca
     3781 cctttatcgt tcattctggc ggtgcggata ttggtactgg tttggatact gtggtgacga
     3841 aactggcagc agaagtgctg cactgccccc cgcaggacgt gcatgttatc tccggtgata
     3901 ccgatcatgc gttgtttgat aaaggcgcat atgcctcgtc cggtacttgc ttctcgggta
     3961 acgctgcgcg tttggcagcg gaaaatctgc gggagaaaat cctgttccac ggcgcgcaaa
     4021 tgttgggtga gccagtggca gatgttcaac tagcaacgcc gggcgtcgtt cgcggcaaga
     4081 aaggcgaagt tagtttcgga gagatagccc ataaaggcga aaccggcacc ggctttggtt
     4141 cactggtggg aactggcagt tatatcacgc ctgatttcgc cttcccgtat ggcgcaaact
     4201 tcgctgaagt tgccgtcaac acgcgtacgg gtgaaatccg cctggataaa ttctacgcct
     4261 tgctggactg cggtacaccg gtcaatccag agttagcgtt gggacaaatc tacggtgcca
     4321 ctctgcgagc tatcggccac agtatgagcg aagagatcat ttatgacgcc gaaggtcacc
     4381 cgttaacgcg tgatttacgc agttacggcg caccgaaaat tggtgacatt ccgcgtgatt
     4441 tccgcgctgt gctggtgccg agcgacgata aagtcggccc attcggggcg aaatcgatct
     4501 cggaaatcgg tgtaaatggc gccgctccgg cgattgctac cgcaattcac gatgcctgcg
     4561 gcatctggtt acgcgaatgg catttcacac cggagaaaat actcaccgcg ctggaaaaaa
     4621 tataagtaat aaatggccgc ttcggcggcc tgtttctctg tttgaacgat gactaaatag
     4681 tgactgcgaa aatgagataa gccgttctca aggcgtcctg cgccccaaat tcgtgggaat
     4741 ttattttact aattcagatg atcaaattta ctttaaaagg agtgaaggga tgtctgatat
     4801 aaaccatgca ggttctgacc ttatatttga actggaggat cgccctccct ttcatcaggc
     4861 tctcgtaggt gccattaccc atctgttggc aattttcgtt ccgatggtaa cccccgcgtt
     4921 aatcgtgggt gcggccttac agctttccgc tgaaacaact gcctatcttg tttctatggc
     4981 gatgatcgcc tctggtattg gtacctggtt acaagtaaac cgctacggca tcgtcggttc
     5041 tggtttactt tcaattcagt cagtcaattt ttcatttgtt acggtcatga ttgcgctggg
     5101 tagcagcatg aaaagcgacg gttttcacga agagttaatc atgtcgtcgc tcctcggcgt
     5161 ctctttcgtt ggcgcatttc tggttgtcgg ctcttctttt atcctgccct atttacgtcg
     5221 ggttattacg cctaccgtca gcggcattgt ggtgctgatg atcggcttaa gcctgattaa
     5281 agttggcatt attgattttg gcggtggttt tgcagctaaa agcagcggta cgttcggcaa
     5341 ttacgagcat ctcggcgttg gcttattggt tttgattgtg gtgatcggct ttaactgctg
     5401 ccgcagtccg ttgctacgca tgggtgggat cgccattggg ctatgtgtcg gctatatcgc
     5461 atcgttatgc ctgggcatgg tggatttcag cagtatgcgc aatttgccgt taatcaccat
     5521 cccgcatccg ttcaaatacg gctttagttt tagcttccat cagttcctgg tggttggcac
     5581 gatttatctg cttagcgtgc tggaagctgt cggcgatatc accgccacgg caatggtttc
     5641 ccgccgtccc attcaggggg aagagtatca gtcccgactg aaaggcggcg tgctggcaga
     5701 tggtctggtt tctgttatcg cctccgctgt cggttcatta ccattaacca cgtttgcgca
     5761 aaataatggg gttattcaga tgactggcgt cgcttcacgt tatgtcgggc gaaccatcgc
     5821 ggtaatgctg gttatcctcg gcttatttcc gatgattggc ggctttttta cgaccattcc
     5881 ctcggcagtt ctgggaggcg caatgacgtt gatgttttcc atgattgcca tcgcagggat
     5941 tcgcatcatc atcaccaacg gtttaaagcg ccgagaaaca cttattgtcg ccacttcttt
     6001 aggtttagga cttggcgtct cctacgatcc cgaaattttt aaaatattgc cagcctctat
     6061 ttatgtatta gttgaaaacc ctatttgtgc tggcgggtta actgcgattt tattaaatat
     6121 tatcctccct ggtggctacc gacaggaaaa cgttctgcct ggtattacct cagcggaaga
     6181 gatggattaa cagtaaagga gtcaatgatg tcaggagaac acacgttaaa agcggtacga
     6241 ggcagtttta ttgatgtcac tcgtacggtc gataacccgg aagaaattgc ctctgcgctg
     6301 cggtttattg aggatggttt attgctcatt aaacagggaa aagtggaatg gtttggcgaa
     6361 tgggaagacg gaaagcatca aattcctgac accattcgcg tgcgcgacta tcgcggcaaa
     6421 ctgatagtac cgggctttgt cgatacacat atccattatc cgcaaagtga aatggtgggg
     6481 gcctatggtg aacaattgct ggagtggttg aataaacaca ccttccctac tgaacgtcgt
     6541 tatgaggatt tagagtacgc ccgcgagatg tcggcgttct tcatcaagca gcttttacgt
     6601 aacggaacca ccacggcgct ggtgtttggc actgttcatc cacaatccgt tgatgcgctg
     6661 tttgaagccg ccagtcatat caatatgcgt atgattgccg gtaaggtgat gatggaccga
     6721 aacgcaccgg attatctgct cgacactgcc gaaagcagct atcaccaaag caaagaactg
     6781 attgaacgct ggcacaaaaa tggtcgtctg ttgtatgcga ttacgccacg cttcgcccct
     6841 acctcatctc ctgaacagat ggcgatggcg caacgcctga aagaagaata tccggataca
     6901 tgggtacata cccatctctg tgaaaacaaa gatgaaattg cctgggtgaa atcgctttat
     6961 cctgaccatg atggttatct tgatgtttac catcagtacg gcctgaccgg taaaaactgc
     7021 gtctttgctc actgcgtcca tctcgaagaa aaagagtggg atcgtctcag cgaaaccaaa
     7081 tccagcattg ctttctgtcc gacctccaac ctttacctcg gcagcggctt attcaacttg
     7141 aaaaaagcat ggcagaagag agtcaaagtg ggcatgggaa cagatatcgg tgccggaaca
     7201 acttttaaca tgctgcaaac gctgaacgaa gcctacaagg tattgcaatt acaaggctat
     7261 cgcctctcgg catatgaagc gttttacctg gccacgctcg gcggagcgaa atctctgggc
     7321 cttgacgatt tgattggcaa ctttttacct ggcaaagagg ctgatttcgt ggtgatggaa
     7381 cccactgcca ctccgctaca gcagctgcgc tatgacaact ctgtttcttt agtcgacaaa
     7441 ttgttcgtga tgatgacgtt gggcgatgac cgttcgatct accgcaccta cgttgatggt
     7501 cgtctggtgt acgaacgcaa ctaataataa aactttaaca tcctcgtgag gacatcatta
     7561 tgtctggaga catcctacaa acaccggacg caccaaagcc acagggcgcg ctggataatt
     7621 attttaaaat taccgctcgt ggcagtaccg ttcgtcagga agtactggct ggcttaacga
     7681 cctttctggc catggtttat tccgttatcg tcgttccggg aatgctgggc aaagcaggtt
     7741 ttcctcccgc agctgtgttt gttgccacct gtctggtcgc gggcttcggc tcgttgctga
     7801 tggggttatg ggccaatttg ccaatggcga ttggttgcgc tatttcctta acggcgttta
     7861 ccgcattcag tctggtactc gggcaacaaa ttagcgttcc tgtcgcactg ggcgcagtct
     7921 ttctgatggg cgtcatcttc accgctattt ccgtaaccgg tgtgcgtacc tggatcttac
     7981 gtaatttgcc gatggggatc gctcacggta caggtatcgg tatcggcctg tttctgctgc
     8041 tgattgctgc taacggtgtg ggaatggtta tcaaaaaccc gattgaaggc ttgccagtgg
     8101 cgctcggtgc ctttacctcc ttcccggtga tgatgagctt actggggctg gcggtcatct
     8161 tcggtctgga aaagtgccgc gtacccggcg ggatcttgtt ggtgattatt gcaatttcga
     8221 tcatcggctt aatctttgac ccagcggtga agtaccacgg tctggtggcg atgccaagcc
     8281 tgactggcga agatggtaag tctctgattt tcagcctcga tattatgggc gcactccagc
     8341 caactgtact tccgagtgta ctggcattgg tgatgaccgc agtgttcgac gccactggca
     8401 ccatccgtgc cgtcgccggt caggcgaatt tgttggataa agacaaccag atcatcaacg
     8461 gcggcaaagc cctgaccagt gactcagtaa gttcaatatt ctccggcctg gtgggcgcag
     8521 cgcccgcgg
//
LOCUS       DAWDRC010000096         8514 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4077_length_8514_cov_2.108760, whole genome shotgun sequence.
ACCESSION   DAWDRC010000096 DAWDRC010000000
VERSION     DAWDRC010000096.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4077_length_8514_cov_2.108760"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1696..1705
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2899..2908
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4162..4171
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7859..7868
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2095 a         2423 c         2098 g         1858 t
ORIGIN      
        1 cttccttccg gacctgacct ggtaaacaga gtagcgttgc gggagaacca acagagcccc
       61 cattgagagc attgagaacc aacgcgcagg cgcattatca ctgctgcccc ggctaattgc
      121 aacccattgc gtgccagatg ctggtttctt gcgcaatgcg gcttcactcg ggcgtttttc
      181 cagattatgc tgaaaaatta cctcaggaag aaaagatgga aaaagaagat tcatttcccc
      241 agcgcgtctg gcaaatcgta gccgctattc ccgaaggcta tgtcaccact tacggtgatg
      301 tggcgaaact ggcgggatcg ccgcgcgccg cgcgccaggt aggcggtgtg ttaaagcgtc
      361 tccccgaagg cagcaccttg ccatggcacc gggtggttaa tcgccacggc accatttcac
      421 tgaccggacc ggatttacag cgtcagcgac aggcattact ggcagaaggt gtgatggtgt
      481 cgggaagcgg gcaaatcgac ttgcagcttt atcgctggaa ctactaaccc tctccgggcg
      541 gagagggtta acttcgctta atattgcgtt ggagctggaa ctgccgaaga cggattcacc
      601 tgggttggtg aagttgaagg tacggtagtc gttgcgccac cgctagcctg aaccggcacg
      661 gcggtttgct gcaccggcac caatgtcagg tcggctttag ttccgccctg gttgatcacc
      721 ggctgaacgg tatcggtaat aaataccagt ttgtcattca cggtaatcgc cgcactcaac
      781 agaatacgcg cgttcggctg aacatctgcc gggttaaatg gcagaacaaa gctgaatggt
      841 gactgtttac cttcggtacg caccgctttc tgcgccagca ctttcgacgg cgcatcggct
      901 aacgacgcgt cagaaagtgt cacggtcagc acggcatcag gcggcagtgc gactttctga
      961 cggatccaaa cggtaccgga gacattcggt tgctggatag caggttgttg tgttgctgaa
     1021 atagacgtat ttgcagccgg tgctggcgtc tgaatatccg cgcttttatc tgcgcaagcc
     1081 gccaacgcaa tcgcaaccgc taaaccactg gccatgtgca cgagtttcat tcacttctcc
     1141 ttattatcaa tgcaccagcg ggctaacttt cctcgccgga agagtggtta acaaaatagt
     1201 aacgtcaaca agtgtggcac acatcacgca tttctgcctg taattagccc gtaattcaga
     1261 ccattgcacc catcggacca gtggcaaatt tgcgttatac tcaactcact ttggcttgct
     1321 gcggcagctt tgttactgga gagttatatg agtcaggcgc taaaaaattt actgacattg
     1381 ttaaatctgg aaaaaattga ggaaggactc tttcgcggcc agagtgaaga tttaggttta
     1441 cgccaggtat ttggcggcca ggtcgtgggt caggccttgt atgctgcaaa agagaccgtc
     1501 cctgacgagc ggctggtaca ttcttttcac agctactttc ttcgccctgg cgatagtaag
     1561 aagccgatta tttatgatgt cgaaacgctg cgtgacggta acagcttcag cgcccgccgg
     1621 gtttctgcta ttcaaaacgg caaaccgatt ttttatatga ctgcctcttt ccaggcacca
     1681 gaagcgggtt tcgaannnnn nnnnngtttc gaacatcaaa aaacaatgcc gtccgcgcca
     1741 gcgcctgatg gcctgccttc agaaacgcaa atcgcccaat cgctggcgca cctgctgccg
     1801 ccagtgctga aagataaatt catctgcgat cgtccgctgg aagtccgtcc ggtggagttt
     1861 cataacccac tgaaaggtca cgtcgcagaa ccgcatcgtc aggtgtggat ccgcgcaaat
     1921 ggtagcgtgc cggatgatct gcgcgttcat cagtatctgc tcggttatgc ttctgatctt
     1981 aatttcctgc cggtagctct acagccgcac ggcatcggtt ttctcgaacc ggggattcag
     2041 attgccacca ttgaccattc catgtggttc catcgcccgt ttaatttgaa tgaatggctg
     2101 ctgtatagcg tggaaagcac ctcggcgtcc agcgcacgcg gctttgtgcg cggtgagttt
     2161 tatacccagg acggcgtgct ggttgcctcg accgttcagg aaggggtgat gcgtaatcac
     2221 aattaaaaaa atagccggag gtgaaaaccg tccggctgtt ttttgcagtg cttgttacgc
     2281 gttataggca ttctcgccgt ggctgttgac gtccagccct tctcgctcct gctcttccgg
     2341 tacacgcaga ccaaccgtca aatccgccag tttgtagccg ataaatgcca ccacaccaga
     2401 ccagacaatc gtaatggcga tgctttccag ctgtaccagc aactgatggc ccatcgtcac
     2461 accttcagcg aagcccacgc cgcccagcga gctggcggca aaaatcccgg tcatgataca
     2521 gccgacaata ccacaaacgc cgtgcacacc gaagacatcg cagggatcat ccacccgcag
     2581 caagcgtttg agcatggtaa cgccccacaa gcccgccaga ccagctacca cgccgataat
     2641 caacgcaccg ccaaccccaa tgtaaccaca ggctggcgtc acgccgacca gaccggcaat
     2701 cgcgccagaa cacgctccca gcagtgaagg cttaccacgc agcgcccatt caccgaagat
     2761 ccagccaaga attgccgccg ccgttgcgac cacagtattc acaaatgcca gtgctgcgat
     2821 ttcattcgcc gtgcccgctg acccggcgtt aaagccaaac caaccgatat agagaatggc
     2881 agtcccggtg aagaccatnn nnnnnnnntt tgcctatcag atacgcgccc accagaccgg
     2941 cgattgcggc gttaatgtgc actaccgtgc cacccgcgaa atccagcgca ccgtgagaag
     3001 ccagcaaacc gccgccccag accatatgcg caatcggaat gtaagagagc gtcagccata
     3061 ccaccacaaa aatcaacaca gctgagaagc ggattcgttc cgccagcgcc ccaactatca
     3121 agcctacggt aatgcaggca aacgatccct gaaacgccac gtggatatac tgataaatgc
     3181 tgcccatcac cgccgtcagt tcaatgtttt tcagcatcaa cccgttaatg ttgccgaaga
     3241 agttgttgcc ttcgccaaac gccagcgagt agccgtaaac cacccagaga atacagacca
     3301 gtgcaaacgt cacggtcacc tgcgtcagca tcgacagcac gtttttgcca cgaatcagcc
     3361 ctccataaaa cagggcaatc cccggaacgg tcataaacag caccagcgca gtacaaatca
     3421 tcataaacgc attgtcggct ttatcggcca ccgcaggtgc agccattacc agtcccggca
     3481 gcatcgccag tgaagtaagc ccagttttta tcgttgctat cttcattttt cgttcctgtt
     3541 gctgtgtgcc agagattaca gcgccgcttc gtcggcttcg ccggtacgaa tacgaatgac
     3601 gcgttgcaat tcagcgacga agattttgcc gtcgccaatt tttccggtgt aagccgcttt
     3661 gctgacgata tcgatcactt catcgagttg gtcatcagca atcgccacat caatttttac
     3721 ttttggcagg aagttgacgc tgtattccgc cccccggtac agctcggcat gccctttctg
     3781 acgcccgaaa cctttcactt cggtgacggt caggccctga ataccaatgg aagataacgc
     3841 ttcacgaacg tctcccagct tgaatggttt gattatcacg gtcaccagct tcatagatcc
     3901 cctccggtca gaattcggta atggttctgc tacacgaaga aggtattgca agcggtgtgc
     3961 cagaaatgaa aaacgagaaa gagagcagga agttaagaag attgaaaatg aaaacgcact
     4021 atgacagtgc acagtgcgtt acatttttgc accaacgatg aaatattgcg ctattcaggc
     4081 gctcaatgat tcctcttcac gcacgctggc tgccagctct tcgcccgcaa gttgcagttg
     4141 atacatctgc cagtatcgtc cnnnnnnnnn nctggtgagt gccctgctcc acggcttgcc
     4201 cacgatgaag caccagaatg gtgtcggcat caacaatagt cgataagcgg tgagcaatca
     4261 ccacaagcgt agtatgttca cgcaccgccg ccagagcatg ttgaatcgcc tgttcagtcc
     4321 cggagtcaat gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc tccaccagca
     4381 cgcgcgccag tgccagcagt tgcttttgcc cgactgagag attattcccc tgctcgccaa
     4441 gcggcgtgta aataccatca ctcatgctac gcgccagctc cgccagctgc acggtttcca
     4501 gcgcctgcca gacgcgttct tcagagatat cacgtcccag cgtcacgttg gcgaggaagg
     4561 tatccgccag caccaccgga tcttgctgca ccattgccac gccctggcgc agcgcgctgt
     4621 gacttagcgt acttaatgga cgaccatcaa ggcgaatctc accttccgtt agcgggtaat
     4681 agcccatcaa taaactggcg agggtgcttt tgccactgcc ggtatgcccg accagcgcca
     4741 caaagttgcg cgaaggcaca gagagattaa tgttctttag caccagattg tcatcgcgat
     4801 aagcaaatga cacgttatcg acgtcgatgg tgccgctctg caacgggcga tcatcattgc
     4861 catattgctg gcgcggtccg tccatcagct caaaaacgcg ctcaccagca acaacagcct
     4921 gttgcagcat cgcctgctgc gtggtcagtt cgattaaggg ttcgttaagt cgccccaaat
     4981 aactgatgaa cgcatacaac acgcccactt caatggtgcc gctggcggag aagccaaaca
     5041 gcatcaacaa gccacaaaga atgagcgatg aaaacagact cagcagcgga cgcagcagaa
     5101 aaccgtcgag gcgcagggtt tgcatcctcg ccatatagtg tgaacgactg gcctcaccca
     5161 tacgttcgcc aaatcgcgcc tgctgacgga actgctggat aacgctcatg ccattaatga
     5221 tttcgttaaa gccgtcgttg atatccgcca gataagcgcg cacacgacgg acaatcggcg
     5281 tgctgtaacg ctgatatatc accattacca ccagcaccac cgggaaaatc attatcgcca
     5341 ccagcgccat tcgccagtcg aggctgaaca ttgccaccag catcgcgccc accagtgcgg
     5401 cgctacgcag gacagttgcc actacggtaa cgtagaggtc gcggatcact tcagtgtcat
     5461 tagtgacgcg ggaaatcacc tgcccgacgg gttgggtatc aaactcgctt aatggctggc
     5521 gtaacgcagc atccatcacg tcggtacgca actgttgcac tacgcctact gccgcccgat
     5581 taaacagcag cgactgcgcg taatgtagcc cggcggcaaa cagttgcagc ccaacatacg
     5641 ccgcagccag ccctgcaacc actttcaacg gcaggttatt tttcgctacc atattgtcga
     5701 taaaatagct gataagcagc ggcccactga cttctgccgc cgccgcaacc cacatcatca
     5761 ggaccgcaat ccccaacggt ttacgccacg gcgaaccgta cgctaacagg cgcttgagag
     5821 tcggccacag ttggctaaaa ctacgcatcg atggcctcct cgcgaatttc cggagcgtcg
     5881 tcgagcgccg cctccagttg ttgatagcga tacatatcgc gataccagcc actttgttgc
     5941 gccagctcat catgattgcc acgctgggcg atatgtccgt gctgcatcac aataatttca
     6001 ctggcttccg tcagtgcaga aaggcgatgg gcactgatga ttaccgttct tccctgcccc
     6061 cactgacgca ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc tgaaagcgca
     6121 tcatcaagga tgaggatttc cgcgttgact aataacgcac gagcaatgga gatacgctgt
     6181 ttttgcccac cggaaagcat cacgccgcgc tcgcccactt ctgtatcgta accttgcggt
     6241 aaacgcaaaa tatcgtcatg cacgctggct aaccgcgcga catgctcaat ctcttgctgg
     6301 gtggcattcg ggcaacccaa cgcgatgttg ttcgctacgg tgtcggaaaa aaggaacggt
     6361 gtctggctaa ctaccgccag gcggctacgc cagctatcga gttgtaactt cgtcagagga
     6421 atatcatgaa aacggatatc accctcgctg acgtcgaaat gacgctgaat gagcgacaac
     6481 agggtacttt tgccggaacc agtcggcccg caaataccta ccatctgacc gggtttcagg
     6541 gcgaaattga cgttttccag cgcaggatgg tcagtctgcg gatacgtaaa ctggcgaata
     6601 ttcacatcca gttcaccacg accttctggc accttatcac taccatcaat aacgactggc
     6661 gcttcggcca gcatggtacg aattcggcta tacgccgcgc tgccacgttc cacaatgtta
     6721 aacatccatg ccagcgccag cattggccaa atcatcagac ctaaatacat tataaaactg
     6781 gtgagttggc ccagcgttaa actgccctgt accaccatcc aactaccgcc gccaatcgcc
     6841 agcaagttcg ccataccaat cgcgatatag atggtcgggt cgaaacgagc atcaatacgc
     6901 gccacccgca tgtttttttt tgccggtatt ttcggcatcc gcggcaaata acgccgactg
     6961 acgatcttcc agaccaaagg ctttgatcat gcgaatactg gtgaggcttt cctgggtgcg
     7021 gtcattaaga ctggaaaacg ccgcctgtgc cagcttaaag cgttcatgca aggcatcgcc
     7081 gttgcgcttg atcatgatcg ccatcactgg catcggcaac agggcaaata aggtcaactg
     7141 ccagctaatt tgcgtagaca tcataatcaa cacagcgccc cttctccggc ggcaaacacg
     7201 acgcgatcga cgtcattggt cgcacgagcc atgaggtcac ccgtgcgatg acgcaggtaa
     7261 aactcaggat gctgccggct tagctggcgg taataatctt cacgcagttc aacagccagt
     7321 tgataagacg caccaaacag caatacgcgc cagacgtaac gcaggagata aaccacaacg
     7381 gcaatcagca ccatggtggc gatccacatc aggatctgcc cggtagtaaa gtgttgttct
     7441 gtcacgccat caacaacaat accaaccact tttggcggaa ccagttgcag catcgcgata
     7501 atgacaagca aggcgacagc cccgagatag cgacgccatt cccgacggaa ataccagctt
     7561 aattgagcaa ataatcgcac gcgttgtgtc ctgacttgat tctggatatt tattcgatgg
     7621 gtaaagaagt ggtgtattta atctgttcca tcgcgaagct ggaagtgacg tccgacagcc
     7681 ccggtacact gtttaccaga cgcttataaa actcgtcgta gcgtttcatg tcggcaacct
     7741 ggacgcgcat cagataatcg tattcaccag ccatgcgcca gaaccccagc acttctggca
     7801 tttcggtaac caccgtgaca aagcggcaat accattcgct gctgtgatgt tgcgttttnn
     7861 nnnnnnnngg tcaggccgag gcctattttt tccggatcca gcagggcgac tttgccaata
     7921 aggataccgt cgtcctccag ccgtttcaga cgcttccagc agggggtggt tgtcagatta
     7981 acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgtagcaa ggccagcagc
     8041 ttacggtcaa ttttatctaa catagccctt ccacagagaa tttttttctc ttttaattct
     8101 attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taagcaccac ggcacaaaat
     8161 gacaaattca acgcaaaaaa aaccgggcaa ttgcccggtg ctggaaggga tctcgttatt
     8221 cgggggaata aggtagatgt ggatagtcca gccagtgcgt caaatagtga gagacagcct
     8281 gatttcggca atgtccaatc accggtaaat gcgggagctc cgcgcgcagt tgcggcatcg
     8341 cattgcccat aataaatccg ctaccgacgc tgcctaacat ttcgcgatcg ttcatcgcat
     8401 caccaaaggc catgcaatcg cgtaacgata aacctaaatg ttgggtcagc accgtcaatg
     8461 cagcgccttt attgcagccc accggcagca cttcgaggca atccgtggcg gaaa
//
LOCUS       DAWDRC010000097         8489 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4089_length_8489_cov_2.544819, whole genome shotgun sequence.
ACCESSION   DAWDRC010000097 DAWDRC010000000
VERSION     DAWDRC010000097.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4089_length_8489_cov_2.544819"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1762..1771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2219 a         2124 c         2121 g         2015 t
ORIGIN      
        1 ccatgtccgg tcaaaccgta gaagtgctga ataccgacgc cgaaggccgc ctggtactgt
       61 gcgacgtatt aacttacgtt gagcgttttg agccggaagc ggtgattgat gtggcgacgc
      121 tgaccggtgc ctgcgtgatc gcgctgggtc atcatattac tggtctgatg gcgaaccaca
      181 atccgctggc ccatgagctg attgctgcat ctgaacaatc cggtgaccgc gcatggcgtt
      241 taccgctggg tgacgagtat caggaacagc tggagtccaa ttttgccgat atggcgaaca
      301 ttggcggtcg tcctggtggg gcgattaccg caggttgctt cctgtcgcgc tttacccgta
      361 agtataactg ggcgcacctg gatatcgccg gtactgcctg gcgttctggt aaggcgaaag
      421 gcgcaaccgg tcgcccggtg gcgttgctgg cacagttcct gctaaaccgc gccgggttta
      481 acggcgaaga gtaattgtat caggctgtgt tgttattgtc ggatgcggcg tgaacgcctt
      541 atccgaccta cacacagcac ttaacttgta ggcctgataa gacgcggcag cgtcgcatca
      601 ggcgctgcgg tgtatacctg atgcgtattt aaatccacca caagaagccc catttatgaa
      661 aaacgcgacg ttctaccttc tggacaatga caccaccgtc gatggcttaa gcgccgttga
      721 gcagttggtg tgtgaaattg ccgcagaacg ttggcgcagt ggtaagcgcg tactcatcgc
      781 ctgtgaagat gaaaagcagg cttaccgact ggatgaagcc ctgtgggcgc gtccggcaga
      841 aagctttgtt ccgcataatt tagcgggaga aggaccgcgc ggcggtgcgc cggtggagat
      901 cgcctggccg caaaagcgca gcagcagccc gcgcgatata ttgattagtc tgcgaacaag
      961 ctttgcagat tttgccaccg ctttcacaga agtggtagac ttcgttcctt atgaagattc
     1021 tctgaaacaa ctggcgcgcg aacgctataa agcctaccgc gtggctggtt tcaacctgaa
     1081 tacggcaacc tggaaataat ggaaaagaca tataacccac aagatatcga acagccgctt
     1141 tacgagcact gggaaaagca gggctacttt aagcctaatg gcgatgaaag ccaggaaagt
     1201 ttctgcatca tgatcccgcc gccgaacgtc accggcagtt tgcatatggg tcacgccttc
     1261 cagcaaacca tcatggatac catgatccgc tatcagcgca tgcagggcaa aaacaccctg
     1321 tggcaggtcg gtactgacca cgccgggatc gctacccaga tggtcgttga gcgcaagatt
     1381 gccgcagaag aaggtaaaac ccgtcacgac tacggtcgcg aagctttcat cgacaaaatc
     1441 tgggaatgga aagcggaatc cggcggcacc attacccgtc agatgcgccg tctcggcaac
     1501 tccgtcgact gggagcgtga acgcttcacc atggacgaag gcctgtccaa tgcggtgaaa
     1561 gaagttttcg ttcgtctgta taaagaagac ctgatttacc gtggcaaacg cctggtaaac
     1621 tgggacccga aactgcgcac cgctatctcc gacctggaag tggaaaaccg cgaatcgaaa
     1681 ggttcgatgt ggcacatccg ctatccgctg gctgacggcg cgaaaaccgg gacaagggcg
     1741 cttccaggct gcctttccac cnnnnnnnnn nacggtaaag attatctggt ggttgcgact
     1801 acccgtccgg aaaccctgct gggcgatact ggcgtggccg ttaacccgga agatccgcgt
     1861 tataaagatc tgattggcaa atatgtcatt ctgccgctgg ttaaccgtcg tattccgatc
     1921 gttggcgacg aacacgccga catggaaaaa ggcaccggct gcgtgaagat caccccagcg
     1981 cacgacttta acgactatga agtcggtaaa cgtcatgccc tgccgatgat caacatcctg
     2041 acctttgacg gcgatatccg tgcaagcgcc caggtgttcg ataccaaagg taacgaatct
     2101 gacgtttatt ctagcgaaat cccggcagag ttccagaaac tggagcgttt tgctgcacgt
     2161 aaagcagtcg ttgctgcggt tgacgcgctc ggcctgctgg aagaaattaa accgcacgac
     2221 ctgaccgttc cttatggcga ccgtggtggc gtggttatcg aaccgatgct gaccgaccag
     2281 tggtacgtgc gtgccgatgt actggcgaaa ccggcagttg aagcggttga gaatggcgac
     2341 attcagttcg taccgaagca gtacgaaaac atgtatttct cctggatgcg cgatattcag
     2401 gactggtgta tctctcgtca gctatggtgg ggtcaccgta tcccggcatg gtatgacgaa
     2461 gcgggtaatg tttatgttgg ccgcaacgaa gacgaagtgc gtaaagaaaa taacctcggt
     2521 gctgatgttg cgctgcgtca ggatgaagac gttctcgaca cctggttctc ctccgcgctg
     2581 tggactttct ctacactcgg ctggccggaa aacaccgacg cactgcgcca gttccaccca
     2641 accagcgtga tggtatctgg ttttgacatc atcttcttct ggattgcccg catgatcatg
     2701 atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc aggtgccgtt ccacaccgtt
     2761 tacatgaccg gtctgattcg tgacgacgaa ggccagaaga tgtccaaatc caagggtaac
     2821 gttatcgacc cgctggatat ggttgacggt atttcgctgc cagaactgct ggaaaaacgt
     2881 accggcaata tgatgcagcc gcagctggcg gacaaaatcc gtaagcgcac cgagaagcag
     2941 ttcccgaacg gcattgagcc gcacggtact gacgcgctgc gcttcaccct ggcggcgctg
     3001 gcttctaccg gtcgtgacat caactgggat atgaagcgtc tggaaggcta ccgtaacttc
     3061 tgtaacaagc tgtggaacgc cagccgtttt gtgctgatga acacggaagg tcaggattgc
     3121 ggcttcaacg gcggtgaaat gacgctgtcg ctggcggatc gctggattct ggcggagttc
     3181 aaccagacca tcaaagcgta ccgcgaagcg ctggacagct tccgcttcga tatcgccgca
     3241 ggcattctgt atgaattcac ctggaaccag ttctgtgact ggtatctgga gctgaccaag
     3301 ccggtaatga acggtggaac agaagccgaa ctgcgcggta ctcgccatac gctggtgact
     3361 gtactggaag gtctgctgcg cctcgcgcat ccgatcattc cgttcatcac cgaaaccatc
     3421 tggcagcgtg tgaaagtact ttgcggtatc actgccgaca ccatcatgct gcagccgttc
     3481 ccgcagtacg atgcgtctca ggttgatgaa gcggcactgg ccgacaccga gtggctgaaa
     3541 caggcgatcg ttgctgtacg taacatccgt gcagaaatga acatcgcgcc gggtaaaccg
     3601 ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac gtcgcgtaaa tgaaaaccgc
     3661 ggcttcctgc aaacgctggc gcgtctggaa agcatcaccg tgctgcctgc tgatgataaa
     3721 ggtccggttt ccgttacaaa gatcgtcgac ggcgcagaac tgctgatccc gatggcaggc
     3781 ctcatcaaca aagaagatga gctggcacgt ctggcgaaag aagtagcgaa gatcgaaggc
     3841 gaaatcagcc gtattgagaa caagctggcg aacgaaggct ttgtcgcccg cgcaccggaa
     3901 gcggtcatcg cgaaagagcg tgagaagctg gaaggctatg cagaagcgaa agcgaagctg
     3961 attgaacagc aggctgttat cgccgcgctg taatactcat agtgaaaacg aaggccggag
     4021 catgctccgg cctttttatt tagataaacg gaacatacgg cataataccg cgagatatcc
     4081 acattaacga accgctatcc tgcggtttgt catcattggt taactccagc gaatcaaggt
     4141 cgccctggac atgggtattg ttagcaatcc agctaaccat ccacattttc agccagggat
     4201 aagccagacc acaggtaatt gaggacccca ttaataccgc caacatgcgc agaagcatac
     4261 cgatcgcggt aacagatgaa tgaaaacgaa ttgtcccatt tgccagcgtc agattattca
     4321 aaaacagatt gcgtaacgtg acatagagat aactggcaaa aacaaggatc gcgacaaaat
     4381 acaggaaata agaagccata atctgcgggt aatattgtaa tacaaactca ctgccagcat
     4441 cagacctacc caacatggtc atcatcatta actgctggaa tactggtgcg atcataatac
     4501 caatcacaat gatgaaagga accatggtta atattgcgag catgcaacct ttaatgcatt
     4561 cttttacgtt tacctgaata gaaaacttat gaatgccaaa agttgcatta ttacccagca
     4621 atggcatcca tttactatag gttataccat ggacaacacc tagcccgatg gcagaaagca
     4681 aacttaatgc gactaagtta aagaataacc ctcctattga tgtcgtcact tgtgcaataa
     4741 gatacagtgc gaaccaaaaa actaacgcca acagaacagg cagccctagc ataacccacc
     4801 atgcacggag cattgagcaa ttaaagccaa agcgaatgcc atttagtgaa gtcatattcg
     4861 cctgatactg taggctcttc actgccatac aaggaatacc gcagatcaag agagcaaaca
     4921 ggaataaacc gagcgcaaag tattgacctg caaaacaaga aatactgaaa agataaaccg
     4981 ccacaataag taacatgcta acaaaaatgg caccacctgt tcccttataa ctaaatggtt
     5041 gccccttgag tgtcacatgt tgataaatat aacgacggca ttttatcaat gcccacggcc
     5101 catatattcc cagagtaata attgtcagta aaaaattcac aagacaaata agaaaatact
     5161 caccaccttt gcctgtgaaa acaaacgagt gccgagaatt atctttcccg atgtttacat
     5221 cattcatgaa ctatatccct aaaataaatt ccattcatac cgataataat tccagtcaat
     5281 tgatactggt tcgcctagaa tacgctttat ctcagagaga tatgcgtaat attttctgat
     5341 ttatcttctt cttgcaccac ctaagcgagg gtggtattaa agtgagctat aaatcataca
     5401 tttgaaattg agctatgcta tgaatgttgt cgcttctccc gcactccgtt tgcgcaaact
     5461 taccgtcgcc gataatcctg ctatcgccca cgtcattcgt caggtatccg ccgaatacgg
     5521 tcttaccgct gataaaggct acaccgtcgc cgatccgaac cttgacgagc tgtatcaagt
     5581 gtatagtcag cctggccacg catattgggt ggttgagtac gagggtgaag tggtcggcgg
     5641 cggtgggatt gcgccattaa ccgggagtga gtcagacatt tgcgaactgc aaaagatgta
     5701 ttttcttccg gctatccgcg gcaaagggct ggcaaaaaaa ctggccttaa tggcaatgga
     5761 gcaggcgcga gaaatgggtt tcaaacgctg ctatctggaa acgaccgctt ttttaaagga
     5821 agccattgcg ctttatgagc atttgggctt tgagcatatc gactatgcgc ttggctgcac
     5881 gggtcatgtc gattgtgaag tacggatgct acgcaaactt tgaaaaatgc ccgcgagctt
     5941 agactcgcgg gcattcgaac attaaccacg tgctttttga gtatgctgga tgtgacgtaa
     6001 atgccttatc cgggctacaa attcaccagg ttgcaggaac tacctgggcc tgatatgcgt
     6061 agcgcatcag gtaacgcagc ctttgtcgtc aacgcttaat tagtgacgaa taccgtcatc
     6121 gtcttcatcg acaaaatctt catcgtcgcc gtcttcgccg ttaggatctt caaagtaagt
     6181 gccccaacca tcgtactcaa cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg
     6241 ggcatcgatc aggtcggcat tcaacgcaca ctcgctgagg atgtcgcagc aaatcacgat
     6301 gtcaccatct tcaacttcca gctcttctgg atcggtcacg tcgtaaccga gtttaaacgc
     6361 ttcaactgct gctttttcca gggtttccag atcgtctgcg gaaagatggt gttcgatggt
     6421 gtacagcgcg tccggatcgc tgccatcttc cagtaattct tcaataatca aacgtgtttc
     6481 ttcacgctgt tcttccagtt gttccgggtt tgccatggct tattcctctt aatgtcctgc
     6541 cgatactctt attgtcacac accactgaca ttgcctccac ctttgtgaca aagatttatg
     6601 ctttagactt gcaaatgaat aatcatccat ataaattgaa ttttaattca ttgaggcgtt
     6661 agccacagga gggatctatg tccgggtttt atcataagca tttcctgaaa ttactcgatt
     6721 tcacgccagc tgaactcaac agcttgttgc agttagccgc gaagctgaaa gccgataaga
     6781 aaagcggtaa agaagaagcc agactcactg gcaaaaacat cgcgctcatc ttcgaaaaag
     6841 actcgacccg tacccgatgc tctttcgaag ttgccgcata tgaccagggt gctcgcgtta
     6901 cttatctcgg cccaagcggc agccagattg gtcacaaaga gtcgattaaa gacactgccc
     6961 gcgtgcttgg tcgcatgtat gacggtattc agtatcgcgg ctatggtcag gagattgtcg
     7021 aaacactggc ggaatacgct ggcgtgccag tatggaacgg cctgaccaat gagttccacc
     7081 ccacgcagct gctggcggat ctcctcacca tgcaggagca tttgcccggc aaagcattca
     7141 acgaaatgac gctggtctat gcaggtgacg cgcgtaacaa catgggcaat tcgatgctcg
     7201 aagctgcggc gcttaccggt ctggatttgc gtctggtcgc gccacaggca tgctggccgg
     7261 aagctgcgct ggtcgccgaa tgcagtgctc tggcacaaaa acacgggggt aaaatcacct
     7321 tgacggaaga tatcgcctct ggggttaaag gcgcagactt tatctatacc gatgtctggg
     7381 tatcgatggg tgagcccaaa gagaaatggg cggagcgtat tgccctgctg cgtgactatc
     7441 aggtgaatag caaaatgatg gccctgaccg gtaattcgca ggttaagttc ctccactgtc
     7501 tgcctgcatt tcatgatgag caaaccacgc tgggtaaaaa aatggccgca gaattcggtc
     7561 tgtatggtgg aatggaagtc accgatgaag tctttgaatc tccggcaagt attgtctttg
     7621 accaggcaga aaaccggatg cacaccataa aagctgttat ggtcgctaca ttagcaaaat
     7681 aacgttttgc cgggcggcgg cacaggtcgc ccccggcagt gatcacttac gattttaata
     7741 gcttaactac tgctttccgt acctgagcgg gggctcccac cgcacaaagc ggtttgtgaa
     7801 cttcgccagg atagaaaacg acaaaatccc cttcattaag aatcactgtt ttttcatcga
     7861 tcccctgccc gataaaagca atatctttat ctgccagcca gtcagtctct ggtacgcctg
     7921 ctggttgcgt gctaaatgtc atcccttcct gcccttttaa tacaatctgg atatcgagat
     7981 aacgagcatg atactcagca cgacgcagtt cacctggttc agtcgtatcc tcagaaatca
     8041 gataaaacag acggtcgccg tcaatagcgt gcttaccttt tgctgtctca tcgctgacat
     8101 gtgatttgat gtactcaatt gcttcgcgaa gttcttccgg cagccaggat tgtaagtgat
     8161 ggatgttgcc aacaatcata atatcttcct ttattaaaac aatgtttcac ttctttcctt
     8221 atacgaaaaa aagccacctc tcaccataga aaaatcatca ggttttgccc ttgcgcatgt
     8281 ttattgctgg agatgttgta ctgatgttaa gcaggagtaa ttttgtatgc agttttgctg
     8341 cataaatctt ataacttagc tatgtatttt ggtcctaata tcttgttatt gcatatgtta
     8401 aaagaaaact ctcagtcaga tcacagttca ttttcttagc ttttaatgcc cgtgctgtca
     8461 tagttatccc gtgaaaatag tgctgtgaa
//
LOCUS       DAWDRC010000098         8394 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4143_length_8394_cov_2.566855, whole genome shotgun sequence.
ACCESSION   DAWDRC010000098 DAWDRC010000000
VERSION     DAWDRC010000098.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4143_length_8394_cov_2.566855"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6074..6083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1902 a         2120 c         2239 g         2123 t
ORIGIN      
        1 cttcctgccg tgcagaaaaa aaatctggcg gtggtcggtg cgggacctgc tgggctggcg
       61 tttgccatta acgcggcggc acgtgggcat caggtaacat tgtttgatgc gcacagcaag
      121 atcggtgggc agtttaatat cgccaaacag atccccggca aagaggagtt ttacgaaacg
      181 ctgcgctatt accgccggat gatcgaagtg acgggtgtga cgctaaaact caatcacacc
      241 gtgacagcgg atcagttaca ggcgttcgat gaaacgatcc tcgccagtgg gatcgtgccg
      301 cgcgttccgc ctatcgacgg gatcgatcat ccgaaggtat tgagttatct cgatgtactg
      361 cgcgacaaag cgccggtggg caacaaagtt gccatcatcg gttgtggcgg aattggtttt
      421 gatacggcga tgtatttaag tcagccgggc gaatccacca gccagaatat cgccgggttc
      481 tgtaatgagt ggggaatcga cagtagcctg caacaggccg gaggtttgag tccgcaaggc
      541 atgcagatcc cccgtagccc acggcagatt gtgatgctcc agcgcaaagc cagcaaacca
      601 ggacaagggt taggcaaaac caccggctgg atccatcgca ccaccctgct ctcacgcggc
      661 gtgaaaatga tcccaggcgt aagttaccag aagatcgacg atgacgggct gcatgtagtg
      721 atcaacggcg aaacgcagat attagcagtg gacaatgtgg tgatctgcgc agggcaagag
      781 ccaaaccgcg cattggcgca accgctgatt gatagtggga aaactgtgca tttaattggc
      841 ggctgcgatg tggctatgga gctggacgca cgacgggcaa ttgcccaggg aacacggctg
      901 gcgctggaga tttaaatcgt tgtttgaagc gccggatgcg acgcatccgg cacgtcgcgt
      961 taatcaataa acaaggcggg agaaatgccg aatcgcgttg ccagtttttt agcgtgttcc
     1021 agcgttaatt tcctcttccc gctcaaaacg cgtgacacca tcgatttact gccaatttcc
     1081 ggaagatcgg aaagggttaa accatattga tccataaggg tacgaatcac agctataccg
     1141 ccaggcatgg cttgcgccat ggcattaaat tcagcaaatt cgggcgctga ttcttcccac
     1201 gcggttattt tggcacacac cagatccagc aaggggtttt caggatcgtt gagcagcaga
     1261 tgatctacca gttccagcgc ctgggtgtat tgttcctcgt tctgaatacc cgccagaaaa
     1321 ggtgccacag cagttaactt ttctcctgct tgcaagatgt cggcaatcgc aatcattttt
     1381 tccccttagt acgatgaacg gcggtaaaga aatcgtattc tttatgcgtc ataacttcac
     1441 gtatgtagca taaacgttga acaacgctaa acaagagaat gtagaggatg gtgacaggga
     1501 ttttttgttt tatggaagcg cgattccaga actgggcaga cattgccgga tgcgacgttt
     1561 gccgcgtctt atccgacctg ggggtaatgt gctgaacgta tgccggataa ggcgtttacg
     1621 ccgcatccgg caggtaaccc ccaatcttac cgacgacgcc ccagcttcac tgctttcagc
     1681 accacaaatt tattattcgt ggcgatggtg gtgcagttgc cgaaaatttt cttcagttta
     1741 tggaagtaat ccaggtgacg gttggcaacg atatacaact cgccgttgat tttcaggcag
     1801 cggcgggcat ggtggaacat ctcccaggcg acgttatcgg tcagcgcatg ttgctgatga
     1861 aacggcgggt tgcagagcac agcattaaag cggaaaggcc ccacgccgga gagcgcgttg
     1921 ttgatcataa actcgcagcg atccaacgcc tcgggcatgt tggtttcaac gttcaaacgg
     1981 ctggaagcaa ccgccatcgg tgattcatcg acaaacacca ctttcgcctg cgggttttta
     2041 tcgagcaatg tcaggccaat aacgccatta ccgcaaccga gatcgacaat ctcaccttcg
     2101 agattctctg gcagatgttg cataaagaag cgcgcgccaa tatccagccc ggtgcgggag
     2161 aagacattcg catggttgtg gatagtccag tcagtacctt ccagcttcca gctaacggtc
     2221 tgcggcgcat cggccaacgg cggttcgttg aaggtgcagt taatcaggcg cgctttcttc
     2281 catgccagcg tggtggtggt cggaccgagc actttttcga acagttccag tgtggaagtg
     2341 tgaatgtcac gggctttggc accggcaata atacgcgtat ctggcgtgac cactttgcgc
     2401 agcgcacgca gttgctgttc cagcaatgcc agtgttttcg gcactttgat cagcaccaca
     2461 cccggctgtt gcgggtagtc ggcggtgctg tcgagaaact tcacgctcga ctcatcaatg
     2521 ccgttgagac gtaaattctc acgcgtcgcc agctcgctga tgtatgagtc accaatgctg
     2581 tagggcttat gttccgccag cacacagctt aacgcaccaa aagcatcatt caggatcaac
     2641 accgggccgc ggatttctgt gtcgtccaac tgttgcagca aatattcatc cgccgcttcc
     2701 cacgcctgta gcgggttaac gtcatccgtc gccggaaaac gttgtagtgt cagtgaacgg
     2761 aaaccgttgt ctaagtggct catcggccct cctgaatgat aaaatttcga cgttgcccct
     2821 gaaaagggtg cgtgagtata ccctttttct ctttttcgtg ggcggttatg agcaatctta
     2881 cttatcttca gggttatccc gagcagctac tttcccaggt gcgaacgctg attaacgaac
     2941 agcgtcttgg cgatgtgctg gcaaaacgtt atccgggaac acacgactac gccactgata
     3001 aagccctctg gcaatatact caggatctta aaaatcagtt tctgcgcaat gccccgccga
     3061 tcaataaagt gatgtatgac aacaagatcc atgtgctgaa aaacgcgctc ggcttacata
     3121 ctgccgtttc gcgtgtgcag ggcggcaagc tgaaagcaaa agcagagatc cgcgtcgcta
     3181 ccgtgtttcg caacgcgccg gaaccgtttt tgcgcatgat cgtggtgcac gagttggcgc
     3241 acctgaagga gaaagagcat aacaaagcgt tttaccagtt gtgctgtcat atggaaccgc
     3301 agtaccacca gcttgagttc gacacccggc tgtggctaac gcagttgtcg cttgggcagg
     3361 acaaaatctg aaaatgatgt caggcgttat tttttcctcc tttagcgcct aaaatcgacc
     3421 tcctcccttt cgttaaggat gacgaccatg ctgcgcgcat ttgcccgcct tcttctccgt
     3481 atttgttttt ctcgccggac tcttaaaatt ggctgtttat tgttgcttat agcaggagca
     3541 acccttttta ttgccgatcg cgttatggtc aacgccagcc aacaactgac ctggagcgat
     3601 gtcaacgccg ttccggcgcg gaacgtgggt ttattgctgg gggcgaggcc gggtaatcgc
     3661 tactttaccc gacgtattga taccgcagca gagttgtacc acgccggaaa agtgaaatgg
     3721 ctgttagtta gtggcgataa cggacgtaaa aattacgatg aagcgtcagg aatgcagcaa
     3781 gccttgatcg ctaaaggcgt accggcaaaa gccatcttct gcgactatgc cggattctca
     3841 acgcttgatt cggtggtgcg tgccaaaaag gtgtttggtg aaaaccatat caccattatc
     3901 tcgcaagaat ttcataatca acgcgccatc tggctggcaa agcaatacgg catcgacgct
     3961 atcggtttta acgcgccaga cctcaatatg aagcacggtt tttatactca actgcgggag
     4021 aaactcgctc gtgtcagcgc ggtgattgac gcaaaaatcc tccatcgcca gccgaaatat
     4081 ctcgggccgt tggttatgat tgggccgttt agtgagcatg gctgtccggc gaaagaataa
     4141 tgcgtatctg cgcacgttga agatgaaaaa ggcgtgctac attgacgaga gaatcccttt
     4201 atggagtatc cacgcgttat gatacgtttc gctgtgattg gtacgaactg gatcactcgc
     4261 cagttcgtcg aggccgccca tgagagcggt aaatacaagt taaccgccgt ttattcccgc
     4321 agtcttgaac aggcccagca cttcgccaat gatttttctg tcgagcatct gtttacgtcg
     4381 ctggaagcga tggcagaaag cgatgccatt gacgcggtgt atattgccag cccgaattcc
     4441 ctgcactttt cccagacaca acttttcctt agtcacaaaa ttcatgtgat ttgcgagaaa
     4501 ccgctggcgt cgaatctggc ggaagtggat gccgccattg cctgtgcgcg ggaaaatcag
     4561 gtggtgctgt ttgaggcatt taaaacggcc tacctgccga actttcatct gttacgccag
     4621 gcgctgccga aagtcggcaa actgcgtaaa gtctttttca actattgcca gtattcctcg
     4681 cggtatcaac gttacctgga tggtgagaat cccaacacct ttaatccggc attctctaac
     4741 ggttcaatta tggatatcgg cttttactgt ctggcgtcag cggtggcgtt atttggtgag
     4801 ccgaaaagcg tgcaggcaac cgccagtttg ttggcaagcg gcgtagatgc ccacggcgtg
     4861 gtggtgatgg attacggtga tttcagcgtt acgttgcagc actcaaaagt gagcgattct
     4921 gtcctggcaa gcgagattca gggcgaagcg ggatcgctgg tgattgaaaa actgtctgaa
     4981 tgccagaaag tttgctttgt tccgcgtggc agccagatgc aggatctcac ccaaccgcag
     5041 catattaata ccatgctcta cgaagcagag ctgttcgcta ccctggtgga tgagcatctg
     5101 gtggatcatc cagggctggc ggtcagtcgc atcaccgcca aactgctgac cgagatccgc
     5161 cgccagactg gggtgatttt tccggcagat agcgtaaaac tataaattgc caaagtaaaa
     5221 cagtgtaaaa ggtatgtaac agcccattga ctgactgaat ggtctgtcat actttgttac
     5281 ctgcaaaggg gagtaacttc attgccggtc gatcgtcatt acgatgtgtg aaaaaccaca
     5341 tccggtcacc gggcaacccg aaaggaatac gcagacgtat tccttttttg ttgtaagtga
     5401 gaccttgccg gaaggcgagg tctatgcata aaaagcagcg gctgacgtct tccgacgttg
     5461 gccgtttttt tatgtgtaag gaacttctat gaatactgtc ggcacgccgt tgctatgggg
     5521 cggattcgct gttgttgtca ccattatgct ggctatcgac ctgttgttgc aggggcgtcg
     5581 tggggcacat gccatgacca tgaaacaggc tgcggcctgg tcgctggtct gggtgacgct
     5641 gtcgttactg tttaacgccg ctttctggtg gtatctggtg caaaccgaag gtcgcgccgt
     5701 tgccgatcca caggcactgg cctttctcac gggttatctg attgagaaat cgctggcggt
     5761 cgataacgtc tttgtctggc tgatgttgtt cagctatttc tctgttccgg cggcattaca
     5821 acgccgcgtg ctggtgtatg gcgtactcgg ggcgattgtt ctgcgtacca tcatgatctt
     5881 cactggtagc tggctgattt cacagttcga ctggatcttg tatatcttcg gtgccttcct
     5941 gctgtttacc ggcgtgaaga tggcgctcgc ccatgaagat gaatcaggca ttggcgacaa
     6001 gccgctggtg cgctggctac gcggtcattt gcgcatgacc gacaccatcg acaacgagca
     6061 tttctttgtg cgtnnnnnnn nnnccgctga tgctggtgct gattctggtg gagttgagcg
     6121 acgtgatttt cgccgtggat agtattccgg ctatcttcgc cgtgaccacc gatccgttta
     6181 ttgtgctgac ctcaaacctg tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg
     6241 caggtgtggc agagcgtttc tcgatgctca aatatggtct ggcggtgatt ctggtgttta
     6301 tcggtatcaa gatgctgatt gtcgacttct accatattcc gatcgctgtc tcgctgggcg
     6361 tggtgtttgg cattctggtg atgacgttta ttatcaacgc ctgggtgaat tatcggcatg
     6421 ataagcagcg ggtagaataa tttttaatct gcctaagccg tgtactctgt catcaacatg
     6481 agcaccgttt tctccctctc ccccaaaagg ggcgagggga ctgaccgagt acttttttga
     6541 cgttgtcatc agtctggaag ccgcacgttg ggctttattt tcatgtcaaa caaatgtaac
     6601 cattaagttg cgaaatatga cttctcttta aaatccagca tatttcgctt cccgaagctg
     6661 taactttcct tatactcgac cttgcaaaca ctttgttaca tcctgaaaga tgcgtcgaca
     6721 gaacgcacca gggatgtgcg acaacacaat gaaaggatcg aaaaatgact acgcaacatt
     6781 caccggggct attccggcgt ctggctcatg gcagcctggt aaaacaaatc ctggccggcc
     6841 ttattctggg gattcttctg gcatggatct caaaacccgc ggcggaagct gttggtctgt
     6901 taggtacttt gttcgtcggc gcactgaaag ccgttgcccc catcctggtg ttgatgctgg
     6961 taatggcatc tattgctaac caccagcacg ggcagaaaac caatatccgc cctattttgt
     7021 tcctctatct gctgggcacc ttctctgctg ctctggccgc agtaatcttc agttttgcct
     7081 tcccttctac cctgcacttg tccagtagcg cgggtgatat ttcgccgccg tcaggcattg
     7141 tagaagtgat gcgcgggctg gtaatgagca tggtttccaa ccccattgac gcgctgctga
     7201 aaggtaacta tatcgggatc ctggtgtggg caattggcct cggtttcgca ctgcgtcacg
     7261 gtaacgaaac caccaaaaac ctggtcaacg atatgtcgaa tgccgttacc tttatggtga
     7321 aactggtgat tcacttcgca ccgatcggta tttttggtct ggtttcttct accctggcaa
     7381 ccaccggttt ctccacgctg tggggctacg cgcaactgct ggtggtactg gttggctgta
     7441 tgctgctggt ggcgctggtg gttaacccac tgctggtatg gtggaaaatt cgtcgtaacc
     7501 cgttcccgct ggtgctgctg tgcctgcgcg aaagcggcgt gtatgccttc ttcacccgca
     7561 gctctgcggc gaacattccg gtgaatatgg cgctgtgtga aaagctgaat ctggatcgcg
     7621 atacctattc cgtttctatt ccgctgggag ccaccatcaa tatggcgggc gcagcaatca
     7681 ccattaccgt gttgacgctg gctgcggtta atacgctggg tattccggtt gatctgccca
     7741 cagcgctgct gttgagcgta gtggcttctc tgtgtgcctg tggcgcatcc ggcgtggcgg
     7801 gggggtctct gttgctgatc ccactggcct gtaatatgtt cggtatttcg aacgatatcg
     7861 ccatgcaggt ggttgctgtc ggctttatta tcggcgtatt gcaggactcc tgtgaaaccg
     7921 cgctgaactc gtcaactgac gtgctgttca ctgcggcagc ttgccaggcg gaagacgatc
     7981 gtctggcaaa tagcgccctg cgtaactaat acttagcccc tttcgtctac ggcggaaggg
     8041 gttttctcca ctttaaacgg atcaattccc cttctctgca tacgccagaa acgaatgata
     8101 ttcaggccat tcatcagcag aaaactgcct tcaatcatcg tgccgcctat cgaccccgcc
     8161 cagaagttgt gaatcaccca gcaacacgtt gaaaaccaca ttacgcagcg catggtcagc
     8221 cctttacagc ggaacagcgc ccaggtactg acaatcgtgc cgattaccgg caatagttcg
     8281 acaggatgat ggaacttcgc gaggccaatt ccgccagtca gcacaataaa aatcgccatt
     8341 acccataagc tgcgcgtgcg taaggtaatc aatgtacgaa tggcattaag gatg
//
LOCUS       DAWDRC010000099         8375 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4155_length_8375_cov_2.652163, whole genome shotgun sequence.
ACCESSION   DAWDRC010000099 DAWDRC010000000
VERSION     DAWDRC010000099.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4155_length_8375_cov_2.652163"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1818..1827
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2739..2748
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2133 a         1925 c         2134 g         2163 t
ORIGIN      
        1 ttaaggggtt ttttgggcgg caataccgcc gctaacgccg agaacgattt ttttaccggc
       61 caggctcatg atgatttttc ctgttgggtg acaccagaag ttggcgattt tatcacaata
      121 cttttgttgt cgtgccttca cctgagattc actttgcgag gcgctttcca ggattgaaaa
      181 ctggccgtcg atttaaagga acggctatga caggatgcga gcaccacaaa ggaggtgaag
      241 gtgaaaaaca atgcacagct gttgatgccg cgcgaaaaaa tgctgaagtt tggtattagc
      301 gccttaacgg atgtcgagct gctggcgcta tttctgcgta ccggaacgcg cggtaaagat
      361 gtattaaccc tggcaaaaga gatgctggag aatttcggct ctctttatgg cttgttaacc
      421 tctgaatatg agcaatttag tggcgttcat ggaattggcg tggcgaaatt cgcccagtta
      481 aaggggattg ccgaactggc gcggcgttac tacaacgtac ggatgcgtga agaaagccct
      541 ttactcagcc cggagatgac gagggaattt ttacaaagcc agctcacggg cgaggagcgg
      601 gagatcttta tggtgatctt tctcgactcc caacacaggg ttataacgca tagccgtctt
      661 ttttccggca cgctaaacca tgttgaagtc catcctcggg aaattatccg cgaagcgata
      721 aaaataaacg cctcggcgct gatccttgcg cataatcacc cttcgggttg tgctgaaccc
      781 agtaaagcgg ataaactcat tactgaacgg ataataaaga gttgtcagtt catggattta
      841 cgcgtgctcg accatatcgt gattgggcgt ggagagtatg tttcttttgc cgaacgcggc
      901 tggatttaac ccgctatgcg cgatccttcg ggatctttgt ctgttcggga cttgagcaca
      961 tcgctgagtc agcgtatact acgccacctt tgagaatctc gggtttggca tttgggcctg
     1021 gcaatcgaga gttcacatag aactgcgatg accgggctgt aaagcctgac gaggcgccaa
     1081 taccccatac gaagctcgag ctaatttgat ttttggagaa tagacatgtc ccgagtctgc
     1141 caagttactg gcaagcgtcc ggtgaccggt aacaaccgtt cccacgcact gaacgcgact
     1201 aaacgccgtt tcctgccgaa tctgcactct caccgtttct gggttgagag cgagaagcgt
     1261 tttgtcaccc tgcgcgtatc tgctaaaggt atgcgtgtaa tcgataaaaa aggcatcgat
     1321 acagttctgg ctgaactgcg tgcccgtggc gaaaagtact aagtacttag aggaaataaa
     1381 tcatggctaa aggtattcgt gagaaaatca agctggtttc ttctgctggt actggtcact
     1441 tctataccac tacgaagaac aaacgtacta agccggaaaa actggaactg aaaaaattcg
     1501 atccagttgt tcgccagcac gtgatctaca aagaagcgaa aatcaaataa tttccgcttt
     1561 gatgtaacaa aaaacctcgc cccggcgggg ttttttgtta tctgcttgcc cccatattga
     1621 ctgcatctgt tcattcctgg agatgctatg cctgaattac ccgaagttga aaccagccgt
     1681 cgcggcatag aaccgcatct cgctggtgca accattcttc atgcggtggt gcgcaacgga
     1741 cgcttgcgct ggccggtttc agaagagatc taccgtttaa gcgaccaacc agtgcttagc
     1801 gtgcagcgcc gggctaannn nnnnnnnggc taaatatctg ctgctggagc tgcctgaggg
     1861 ctggattatc attcatttgg ggatgtctgg cagcctgcgc atccttccag aagaacttcc
     1921 ccctgaaaag catgaccatg tggatttggt gatgagcaac ggcaaagtgc tgcgctacac
     1981 cgatccgcgc cgctttggtg cctggctatg gaccaaagag ctggaagggc acaacgtgct
     2041 ggcccatctt ggaccggagc cgcttagtga cgatttcaac ggtgagtacc tgcatcagaa
     2101 gtgcgcgaag aaaaaaacgg cgattaaacc gtggctgatg gataacaagc tggtggtagg
     2161 cgtagggaat atctatgcca gcgaatcgct gtttgcggcg gggatccatc cggatcggct
     2221 ggcgtcatca ctgtcgctgg cggagtgtga attgttagct cgggtgatta aagcggtgtt
     2281 gctgcgttcg attgagcagg gcggtacaac gctgaaagat tttctgcaaa gtgatggtaa
     2341 accgggctat ttcgctcagg aattgcaggt ttacgggcga aaaggtgagc cgtgtcgggt
     2401 gtgcggtacg ccgattgtgg cgactaaaca tgcgcagcgg gcaacgtttt attgtcggca
     2461 gtgccagaag taattgatgc gcgccggatg gcataccatc cggcataaac gctacgctaa
     2521 cttcgccatc agcgcctgat ggacattctc cggcaggaaa tgggtgacat cgccctgatg
     2581 gcgcgccacc tctttcacca acgatgaaga gataaacgac cactctttcg acggcatcag
     2641 aaacacactt tccagctccg gcattaagtg gcgattcata tgcgccagct gcatttcata
     2701 ttcaaaatcc gccaccgcac gcaggccacg aatcagcann nnnnnnnngg cacgttcgcc
     2761 atcaaatcac taaacccgac cacttccacg ttccccagat gcgcggttgc ctgctgtgcc
     2821 agctccacac gctcttcaag ggtaaacatc ggttttttac tgggactagc ggcaatcgcc
     2881 agaataacgt gatcgaacat ttgcgtggcg cgcgtcacga tatcgatatg accattggta
     2941 atgggatcga aagtacccgg ataaatcgcc cgtttttgca taacaacctc aatgcgtttt
     3001 cggtggcagg taaggttcca gcagttgaag cagacgctgt agtgcgccct ggttttgata
     3061 cagcacttca acggcatgac ggccatagaa actacggtaa tcggcgtcgg tgagtaaaga
     3121 ggaaacctct tttgcaagcg tagtggcatc ggtaacggta atcagcccgc ttgcctgctc
     3181 cagccgcgcg caaatgtctt taaagttaaa cgtatgcggc cccatcaata ccggaatagc
     3241 gtgtgcggcc gcttccagcg gattatgccc accacgttca accagtgaac cgccaacaaa
     3301 ggcgagatcg gcaatgccgt acagtaacat caactcgccc atcgtatcgc caaccacaac
     3361 ctgcgtgctg gtggagggga cttcccctga agatcgtgtg atatagctta gtccagcctg
     3421 gcggacaagg ttaatcgcat ccgggaagcg ttctggatga cggggtacca ggatgagcaa
     3481 taaattcggg aattgctgta acaatgcctg atgtgcggcg atcaccacgc tctcttcgcc
     3541 ttcatgagtg ctggtggcaa tccataccgg gcggtgtggt gcccactggc ggcgcagcgt
     3601 cacggcttta gcagccaact gcggcgttac agaaatatcg aatttcaggc taccggtaac
     3661 ggtcacctga ttattttttg cgcccagcgc cacaaaacga gcaccatctt cttcattttg
     3721 ggcggcaatc agcgtaatac gacgcagcaa gcgacggacg aatttaccca gtttggcata
     3781 acctgcggcc gagcgggcag agagtcgcgc gttagcgatc accagcggaa ttttacgttt
     3841 atgtagcgcc gcaatcaggt taggccatag ttcggtttcc ataatcaaca ccagtttagg
     3901 gtcgacttta ttcaggaaac ggttgagtgc atcgggcagg tcatacggca gataaacgtg
     3961 ctgaacatcc ttcccgaaag ccgattgtac gcgctccgaa ccggttggag tcatggttgt
     4021 tacggtaatc ggtaaatcag gataacgatg acgcagtgcg cgcaccaacg ggatcgccgc
     4081 cagagtttca ccgacggaga cggagtgcag cataatgccg cctggtttta gcggatggcg
     4141 gtaaaaaccg taacgttcac cccagcgttt tcgataggcc ggagccttac gtccgcgcac
     4201 ccagagccgt atccagatca gtggctgaat aaggtagaga agggcggtgt aaagcaattc
     4261 gagcatagta aatagctgac ttatggatgt gctggggatt ctatgtattt agctgtggct
     4321 ttaccattac ttttcccgtt tttgacttaa atagcaattt acccgccaat tcttgctgaa
     4381 catctatact ttttttgtat ttttatgcga ttcattgatt tctgcagtac gtgcaggaag
     4441 aaatcgcaga gcaacagtaa aagaatatgg ttgctaatga ttttatttga ttgatttctt
     4501 tattgaaaat caacaatgtg cttaaaaatc attcgagtcg gattgccgcg aaagaagtga
     4561 tacactagcg cgtcaatatc agtgcggtat cagtattcag gtagctgttg agcctggggc
     4621 ggtagcgtgc tttttctgct tcaccttaat cggataatct caacaaaaag agttacttgt
     4681 ggataagcca tttcgaagaa ttttgctcat taagatgcgt tttcatgggg atatgttatt
     4741 aactactccc gtcattagtt cgctgaaaaa aaattaccct gacgcaaaaa tcgatgtgct
     4801 gctttatcag gacaccatcc cgatcctgtc tgaaaatcca gagattaacg cgctctacgg
     4861 cataaaaaat aaaaaagcaa aagcctcaga aaaaattgcc aactttttcc atctcatcaa
     4921 ggtattacgt gccaataagt atgaccttat cgttaatctt accgatcaat ggatgattgc
     4981 tatactggtt cgcttattaa atgcccgtgt gaaaatttcc caggattatc atcatcggca
     5041 gtctgctttt tggcgtaata gtttcactca tttggtgccg ttgcagggtg gaaatgtggt
     5101 ggaaagtaac ttatccgtgc tgacgccatt gggacttgaa tcgttggtga agcagacaac
     5161 catgagttac ccgcctgcaa gctgtaaacg tatgcgtcgc gaacttgatc acgctggtgt
     5221 tggacaaaat tatgtggtta tccaacctac ggcgcggcaa atcttcaaat gctgggacaa
     5281 cgccaagttt tccgctgtga ttgatgcctt acatgctcgt ggttatgaag tcgttctgac
     5341 gtccggccca gataaagacg atctggcctg cgtcaatgaa attgcgcagg gatgccagac
     5401 gccaccagta acggcgctgg ccggaaaggt gaccttcccg gaacttggtg cgttaatcga
     5461 tcatgcgcag ctgtttattg gcgttgattc cgcaccggcg catattgccg ctgcagttaa
     5521 tacgccgctg atatcgctgt ttggtgcgac ggaccatatt ttctggcgtc cctggtcaaa
     5581 taacatgatt caattctggg cgggagatta ccgggaaatg ccaacgcgcg atcagcgtga
     5641 ccgaaatgag atgtatcttt cggttattcc ggcggcagat gtcattgcag ctgtcgataa
     5701 attacttccc tcctccacga caggtacgtc gttatgatcg ttgctttttg tttatataaa
     5761 tattttccct ttggcggttt gcagcgcgat tttatgcgta ttgctcagac agtcgccgct
     5821 cgaggttatc atgttcgggt ttatgcccag tcgtgggaag gcgaatgccc tgatgtattt
     5881 gaactgatca aagtgccggt taaatcgcat accaatcacg ggcgcaatgc ggagtatttt
     5941 gcctgggtgc aagaacattt acgtgaacat cccgtcgata gagtcgttgg atttaataaa
     6001 atgccggggc tggacgttta ttatgccgct gatgtttgtt atgccgagaa agtagcgcag
     6061 gagaaaggct ttttctatcg cctgacgtca cgttatcgcc attatgccgc ctttgagcgg
     6121 gcaaccttcg aacagggcaa gccgacacag ctgctgatgc tgacagataa gcaaatcgcc
     6181 gatttccaga aacattatca gactgaagcg gagcgttttc atattctgcc accggggatt
     6241 tatcctgatc gtaaatatag ccagcagcca gctaatagcc gtgaaatctt ccgtaagaag
     6301 aatggaataa ccgaacaaca atatttattg ttgcaggtcg gttcagactt cacgcgtaaa
     6361 ggtgtcgatc gttccattga agcacttgct tcgttaccgg attcgctgcg ccacaacaca
     6421 ttgctatatg ttgttgggca ggataaaccg cgaaaatttg aggcgctggc agaaaaacgc
     6481 ggcgtgcgca gtaatgttca cttcttctcg gggcgcaacg atgtctcgga attaatggcg
     6541 gcggcggatt tattacttca tcctgcctac caggaagccg caggaattgt actactggaa
     6601 gcgataactg caggattacc ggtactaaca acagccgttt gtggctatgc gcattatatt
     6661 gtcgatgcca attgcggcga ggctattgct gagccattcc gccaggaaac attgaatgag
     6721 attttacgca aagcgttaac gcaatcttca ttgcgccagg cctgggcgga aaatgcgcga
     6781 cattatgctg atacacaaga tttatacagt ctgccagaga aagcggcgga catcataacg
     6841 ggtggtctgg atggttgaac ttaaagagcc gtttgccacg ttatggcgcg gtaaagatcc
     6901 ttttgaggaa gttaaaacct tgcagggtga ggtatttcgt gaactggaaa ctcgccgcac
     6961 tctgcgcttt gaaatggcgg gcaaaagcta ttttctcaaa tggcatcgcg gcacgaccct
     7021 gaaagagata atcaaaaatt tactctcatt gcggatgcca gtattaggcg cagaccgcga
     7081 atggaatgcg attcatcgac tgcgggatgt cggcgttgat actatgtatg gggtggcatt
     7141 cggcgaaaaa ggcattaatc cgctgaccag aacctcgttt attattaccg aagatctgac
     7201 accaaccatc agtctggaag attactgtgc tgactgggcg accaactcac cagatgttcg
     7261 cgtaaagcgt atgcttatta agcgtgtcgc gacgatggtg cgcgatatgc atgctgcggg
     7321 cattaaccac cgtgacggtt atatctgtca tttccttctg cacttgcctt tttccggtaa
     7381 ggaagaggag ttaaaaattt cagtaattga cctgcaccgg gcgcagatac gtgcaaaagt
     7441 accgcgtcgt tggcgggata aagatcttat tggactttat ttttcttcga tgaatattgg
     7501 cctgactcag cgggatatct ggcggtttat gaaagtgtat tttgtcgccc cgcttaaaga
     7561 cattatcaag caggaacaag ggctgctgtc gcaagcagaa gcaaaagcca caaaaatcag
     7621 ggaaagaacg attcgaaaat cgttgtaatt tccggagaga aatatgagtg cccactattt
     7681 taatccacaa gagatgatca ataagacaat catcttcgat gaaaggccag aggcgtcagt
     7741 ggcatcatca ttccatgttg cttatggcat tgatcagaac tttctttttg gttgtggtgt
     7801 ttcaatcacg tcagttttgt tacataacaa cgacgtgagt tttgtcttcc acgtttttat
     7861 tgatgatatc cctgacgccg atatccagcg tttatcccaa ttggcgaaaa gctatcatac
     7921 ctgtatccag atccatctgg taaattgtga acagcttaag gtattaccga cgaccaaaaa
     7981 ttggtctatt gccatgtatt tccgttttgt aattgcagat tactttattg atcaacaaga
     8041 taagatcctg tacctggatg ctgatatcgc ctgtcaggga accttaaaac cgctgataac
     8101 aatggatctt gccaataacg ttgctgctgt tgttactgaa cgcgatgcta actggtggtc
     8161 gttacgggct caaagtctgc agtgtaatga acttgaaaag ggttacttta attcaggtgt
     8221 cctgttaatt aatacactag cgtgggcgca ggagtccgtt tctgctaaag cgatgtcgat
     8281 gcttgctgat aaagccgtcg tttcccgttt aacctatatg gatcaagata tactaaatct
     8341 tatcctgtta gggaaagtta aattcattga tgcta
//
LOCUS       DAWDRC010000100         8367 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4159_length_8367_cov_2.397618, whole genome shotgun sequence.
ACCESSION   DAWDRC010000100 DAWDRC010000000
VERSION     DAWDRC010000100.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4159_length_8367_cov_2.397618"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3373..3382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7242..7251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2032 a         2043 c         2382 g         1890 t
ORIGIN      
        1 atccgtgtct ggctttctgc gcagaccgga gcggggatac cacagctttt tcaggctttg
       61 acggagcgtc tttccggcga ggtggcgcag catacattgc gtctgccgcc gcaggaaggg
      121 cgcctgagaa gtcgttttta tcagcttcag gcaatagaaa aagagtggat ggaggaggac
      181 ggcagcgtaa gtctgcaagt tcgtatgccg atcgttgact ggcgtcgcct ctgcaaacaa
      241 gaaccggcgt tgatcgatta cctgatctaa cggcgtagcg tctgaagcgt ggagtcatat
      301 cctctggcgt cgaaagacaa cagggatcac cgcataacaa atatggagca caaacatggc
      361 gtggaatcag cccggtaata acggacaaga ccgcgacccg tggggaagca gcaaacctgg
      421 cggcaactct gagggaaatg gaaacaaagg cggtcgcgat caagggccac ctgatttaga
      481 tgatatcttc cgcaaactga gcaaaaagct cggtggtctg ggtggcggta aaggcaccgg
      541 atctggcggt ggcagttcat cgcaaggccc gcgcccgcag cttggcggtc gcgtcgttac
      601 catcgcagcg gcagcgattg tcattatctg ggcggccagt ggtttctata ccattaaaga
      661 agccgaacgc ggcgtggtaa cacgctttgg taaattcagc catctggttg agccgggtct
      721 gaactggaaa ccgacgttta tcgacgaagt caaaccggtg aacgtggaag ccgtgcgtga
      781 actggcagct tctggtgtga tgctgacgtc ggacgagaac gtagtgcgcg ttgagatgaa
      841 cgtgcagtac cgcgtcaccg atccggaaaa atatctgtat agcgtgacca gcccggatga
      901 cagtctgcgt caggctaccg acagcgccct gcgcggggtt atcggtaaat acaccatgga
      961 ccgcattctg acggaaggtc gtaccgtgat tcgtagcgat actcagcgcg aactggaaga
     1021 gacgattcgt ccgtatgaca tgggcatcac tctgctggac gtcaacttcc aggctgctcg
     1081 tccgccggaa gaagtaaaag cggcgtttga cgatgcgatt gccgcgcgtg aaaacgaaca
     1141 gcaatacatt cgtgaagcag aagcgtatac caacgaagtt cagccgcgtg cgaacggtca
     1201 ggcgcaacgt atcctcgaag aggcgcgtgc gtacaaggcc cagaccatcc tggaagctca
     1261 gggtgaagtg gcgcgctttg ctaaacttct gccggaatat aaagccgcgc cggaaattac
     1321 tcgcgagcgt ctgtatatcg agacgatgga aaaagtgttg ggcaacaccc gcaaagtgct
     1381 ggttaacgat aaaggtggca acctgatggt tctgccatta gaccagatgc tgaaaggtgg
     1441 taacgcccct gcggcgaaga gcgataacgg tgccagcaat ctgctgcgtc tgccgccagc
     1501 ctcttccacc acaaccagtg gagcaagcaa cacgtcgtcc accagtcagg gcgatattat
     1561 ggaccaacgc cgcgccaacg cgcagcgtaa cgactaccag cgtcaggggg aataatgatg
     1621 cgtaagtcag ttatcgcgat tatcatcatc gtgctggtag tgctttacat gtctgtcttt
     1681 gtcgtcaaag aaggtgagcg cggtattacg ctgcgttttg gtaaggtact gcgtgacgat
     1741 gacaacaaac ctctggttta tgagccgggt ctgcatttca agataccgtt cattgaaacg
     1801 gtgaaaatgc tcgacgcacg tattcagacc atggacaacc aggccgaccg ctttgtgacc
     1861 aaagagaaga aagacctgat cgtcgactct tacatcaaat ggcgtatcag cgatttcagc
     1921 cgttactacc tggcaacggg cggtggcgac atttcgcaag cggaagtgct gttgaaacgt
     1981 aagttctctg accgtctgcg ttctgaaatt ggtcgtctgg acgtgaaaga catcgtcacc
     2041 gattcccgtg gtcgtctgac cctcgaagta cgtgacgcgc tgaactccgg ttctgcgggt
     2101 acagaagatg aagttactac cccggcggca gataacgcca ttgccgaagc ggcagagcgc
     2161 gtaacggctg agacgaaggg caaagttccg gtcatcaacc cgaacagtat ggcggcgctg
     2221 ggtattgaag ttgtcgatgt gcgaattaag cagatcaacc tgccgaccga agtgtctgaa
     2281 gcgatctaca accgtatgcg cgccgagcgt gaagcggtag cgcgtcgtca ccgttcacaa
     2341 ggtcaggaag aagcggaaaa actgcgtgca actgccgact atgaagtgac cagaacgctg
     2401 gcagaagctg agcgtcaggg ccgcatcatg cgtggtgaag gcgatgcaga agcagccaaa
     2461 ctgtttgctg atgcattcag taaagatccg gacttctacg cattcatccg tagcctgcgt
     2521 gcttatgaga acagcttctc tggcaatcag gacgtgatgg tcatgagccc ggacagcgat
     2581 ttcttccgct acatgaagac gccgacttcc gcaacgcgtt aatataacga ctgcggtaca
     2641 ggtcaataaa gccaccgcat cctcagggat gtcggtggtt ttctttttct ataaggataa
     2701 tgaatgaatt cgacaatctg gctggcgctt gccctggttt tggtactgga aggtttaggg
     2761 ccgatgcttt acccgaaggc atggaagaag atgatctctg cgatgaccaa tttgcccgat
     2821 aatattttac gtcgttttgg cggtggactt gtggttgcgg gcgttgtggt ctactacatg
     2881 ttgaggaaaa cgattggctg aacaaaaaat agactgatcg aggtcatttt tgagtgcaaa
     2941 aagtgctgta actctgaaaa agcgatggta gaatccattt ttaagcaaac ggtgattttg
     3001 aaaaatgggt aacaacgtcg tcgtactggg cacccaatgg ggtgacgaag gtaaaggtaa
     3061 gatcgtcgat cttctgactg aacgggctaa atatgttgta cgctaccagg gcggtcacaa
     3121 cgcaggccat actctcgtaa tcaacggtga aaaaaccgtt ctccatctta ttccatcagg
     3181 tattctccgc gagaatgtaa ccagcatcat cggtaacggt gttgtgctgt ctccggctgc
     3241 gctgatgaaa gagatgaaag aactggaaga ccgtggcatc cccgttcgtg agcgtctgct
     3301 gctgtccgaa gcatgtccgc tgatccttga ttatcacgtt gcgctggata acgcgcgtga
     3361 gaaagcgcgt ggnnnnnnnn nnttatgaag ataaagtagc acgtcgcggt ctgcgtgttg
     3421 gcgacctttt cgacaaagaa accttcgctg aaaaactgaa agaagtgatg gaatatcaca
     3481 acttccagtt ggttaactac tacaaagctg aagcggttga ttaccagaaa gttctggatg
     3541 atacgatggc tgttgccgac atcctgactt ctatggttgt tgacgtttct gatctgctcg
     3601 accaggcgcg tcagcgtggc gatttcgtca tgtttgaagg tgcgcagggt acgctgctgg
     3661 atatcgacca cggcacttat ccgtacgtaa cttcttccaa cactactgct ggtggcgtgg
     3721 cgaccggttc cggcctgggc ccgcgttatg ttgattacgt tttgggtatc ctcaaagctt
     3781 actccactcg tgtgggtgca ggtccgttcc cgaccgaact gtttgatgaa actggcgagt
     3841 tcctctgcaa gcagggtaac gaattcggcg caactacggg tcgtcgtcgt cgtaccggct
     3901 ggctggacac cgttgccgtt cgtcgtgcgg tacagctgaa ctccctgtct ggcttctgcc
     3961 tgactaaact ggacgttctg gatggcctga aagaggttaa actctgcgtg gcttaccgta
     4021 tgccggatgg tcgtgaagtg actaccactc cgctggcagc tgacgactgg aaaggtgtag
     4081 agccgattta cgaaaccatg ccgggctggt ctgaatccac cttcggcgtg aaagatcgta
     4141 gcggcctgcc gcaggcggca ctgaactaca tcaagcgcat tgaagagctg accggtgtgc
     4201 cgatcgatat catctctacc ggtccggatc gtactgaaac catgattctg cgcgacccgt
     4261 tcgacgcgta attctggtac gcctggcaga tattttgcct gccgggctaa cagtgtgata
     4321 cattgctgtg tcggttaagc cattacgcta tccgacacag tgttaaatcc tcgctttttt
     4381 ccttccccga actgaaataa attagcgaca cagcttgtgg ctggtttatc atcaatataa
     4441 atgtattttt ccccgatttc ccttttgagg ttgatgtgca gttaacgagt ttcactgatt
     4501 acggattacg tgcgctgatc tacatggcgt cattgccaga agggcggatg accagtattt
     4561 ctgaagtgac tgacgtctac ggcgtctccc gtaatcatat ggtcaaaata atcaatcaac
     4621 ttagtcgtgc cggctacgtg actgctgttc gtggaaaaaa tggcggcatt cgcttgggta
     4681 aaccggcgag tgcaatacgt attggtgatg tggtgcgcga gctggagccc ttatcgctgg
     4741 tgaattgcag cagtgagttt tgccacatta cacctgcctg tcggttgaaa caggcgcttt
     4801 ctaaggccgt gcaaagtttt ctgacggaac tggataacta cacgcttgcc gatttggttg
     4861 aagagaatca accgctttat aaattattgc tggtggagtg acgaaaatct tcatcagaga
     4921 tgacaacgga ggaaccgaga tgtcacaaga tcctttccag gaacgcgaag ctgaaaaata
     4981 cgcgaatccc atccctagtc gggaatttat cctcgaacat ttaaccaaac gtgaaaaacc
     5041 ggccagccgt gatgagctgg cggtagaact gcacattgaa ggcgaagagc agcttgaagg
     5101 cctgcgtcga cgcctgcgcg cgatggagcg cgatggtcaa ctggtcttca ctcgccgtca
     5161 accgcgggga attctctggc agctcagaac cagagaactg aatatacagg ctgcccactg
     5221 cggtgccgga acgcctcgac ctggtgaaag gtaccgttat tggccaccgt gatggctacg
     5281 gctttctgcg agttgaaggg cgtaaagatg atttgtatct ctccagcgag cagatgaaaa
     5341 cctgcattca tggcgatcag gtgctggctc agccgctggg cgctgaccgt aaaggtcgtc
     5401 gtgaagcgcg tattgtccgc gtactggtgc caaaaaccag ccagattgtt ggtcgctact
     5461 tcaccgaagc gggcgtcggc tttgtggttc ctgacgatag ccgtctgagc ttcgatatct
     5521 taatcccgcc cgatcagatc atgggcgcgc ggatgggctt tgtggtggtg gttgaactga
     5581 ctcaacgccc gactcgccgt accaaagcgg tgggtaaaat cgtcgaagtg ctgggcgaca
     5641 atatgggcac cggcatggcg gttgatatcg ctctgcgtac ccatgaaatt ccgtacatct
     5701 ggccgcaggc tgttgagcaa caggttgccg ggctgaaaga agaagtgccg gaagaagcaa
     5761 aagtgggccg tgtcgatctg cgcgatttac cgctggtcac cattgatggc gaagacgccc
     5821 gtgactttga cgatgcagtt tactgtgaga aaaaacgcgg cggcggctgg cgtttatggg
     5881 tcgcgattgc cgacgtcagc tactatgtgc gtccgccaac gccgctggac agagaagcgc
     5941 gtaatcgtgg tacgtcggtg tacttccctt cgcaggttat cccgatgctg ccggaagtgc
     6001 tctctaacgg cctgtgctcg ctcaacccgc aggtagaccg cctgtgtatg gtgtgcgaga
     6061 tgacggtttc gtcgaaaggc cgcctgactg gctacaaatt ctacgaagcg gtgatgagtt
     6121 ctcacgcgcg tctgacctac accaaagtct ggcatatact gcaaggcgat caggatctgc
     6181 gcgagcagta tgcgccgctg gttaagcatc tcgaagagtt acataacctc tataaagtgc
     6241 tggataaagc ccgtgaagaa cgcggtggga tctcatttga gagcgaagaa gcgaagttca
     6301 ttttcaacgc tgaacgccgt attgaacgta tcgaacagac tcagcgtaac gacgcccaca
     6361 agttaattga agagtgcatg attctggcga atatctcggc ggcgcgtttc gttgagaaag
     6421 ccaaagaacc ggcactgttc cgtattcacg acaagccgag caccgaagcg attacctctt
     6481 tccgttcagt gctggcggag ctggggctgg aattgccggg cggtaacaag ccggaaccgc
     6541 gcgactatgc agagttactg gaatcggttg ccgatcgtcc tgatgcagaa atgctgcaaa
     6601 ccatgctttt gcgctcgatg aaacaggcga tttacgatcc ggaaaaccgt ggtcacttcg
     6661 gtctggcatt gcagtcctat gcgcacttta cttcgccgat tcgtcgttat cctgacctga
     6721 cgttgcaccg cgccattaaa tatctgctgg cgaaagagca ggggcatcag ggcaacacca
     6781 ctgaaaccgg cggctaccat tattcgatgg aagagatgct gcaactgggt cagcactgtt
     6841 cgatggcgga acgtcgtgcc gatgaagcaa cgcgcgatgt gtctgactgg ctgaagtgtg
     6901 acttcatgct cgaccaggta ggtaacgtct ttaaaggcgt aatttccagc gtcactggct
     6961 ttggcttctt cgtccgtctg gacgacttgt tcattgatgg tctggtccat gtctcttcgc
     7021 tggacaatga ctactatcgc tttgaccagg tagggcaacg cctgatgggt gaatccagcg
     7081 gccagactta tcgcctgggc gatcgcgtgg aagttcgcgt cgaagcggtt aatatggacg
     7141 agcgcaaaat cgactttagc ctgatctcca gcgaacgcgc accgcgcaac gtcggtaaaa
     7201 cggcgcgcga gaaagcgaaa aaaggcgatg caggcaaaaa annnnnnnnn ncaagcggcg
     7261 tcaggtcggc aaaaaggtca actttgagcc agacagcgcc ttccgcggcg agaaaaaacc
     7321 gaagccgaaa gcagcgaaga aagacgcgag aaaagcgaaa aagccatcgg cgaaaacgca
     7381 gaaaatagcc gcagcgacca aagcgaagcg tgcggcgaag aaaaaagtgg cagagtgatc
     7441 attaccctct tcaaacagaa gagggtgaat tatccgtagg ccgggcaagc gtggcgctcc
     7501 cggcaaaacg taaacaacga gtacattaat gagcgaaatg atttacggca tccatgcagt
     7561 gcaggccctg ctggagcgcg cccctgaacg ttttcaggaa gtctttattt taaaaggccg
     7621 tgaagataaa cgtctgttac cgctgattca cgcccttgag tctcagggcg tggttatcca
     7681 gttggcaaac cgccaatatc tcgatgagaa aagcgacggt gccgtgcatc agggcattat
     7741 cgctcgtgtg aagccaggac ggcagtatca ggaaaacgat ctgccggatc tgatcgcttc
     7801 gctcgatcaa ccgttcctgc tgatcctcga cggcgtaacc gatccgcaca acctcggcgc
     7861 gtgtctgcgt agcgcggacg ccgctggtgt tcatgcggtg attgtgccga aagatcgctc
     7921 cgcacagctc aacgccacgg cgaaaaaagt ggcctgtggt gcggcagaaa gcgttccgct
     7981 gattcgggtg actaatctgg cgcgcaccat gcgtatgttg caggaagaga atatctggat
     8041 cgtcggtacg gcaggcgagg cggatcatac actctatcag agcaaaatga ccggacgcct
     8101 ggcgctggtg atgggcgcgg aaggtgaagg tatgcgtcgc ctgacccgtg aacattgcga
     8161 tgagttgatc agcatcccaa tggcaggaag tgtttcttcc ctgaacgttt cggttgcaac
     8221 cggaatttgc ttatttgaag cggttcgtca gcgcagctag ttcctctgaa ttatgaaaag
     8281 ccatccagat ttggatggtt tttttttgtc tatagctggt aagataatta cgtactgcaa
     8341 atattcattt tattaatatt taaactt
//
LOCUS       DAWDRC010000101         8358 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4162_length_8358_cov_2.477297, whole genome shotgun sequence.
ACCESSION   DAWDRC010000101 DAWDRC010000000
VERSION     DAWDRC010000101.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8358)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4162_length_8358_cov_2.477297"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2051 a         2031 c         2146 g         2130 t
ORIGIN      
        1 catgaagtag tccatctcct ggctgcggcg cgcttcaagc gtcgcttcca gtcgcgtttt
       61 tgccagcaag aattcattat ttccggccgc aagttcttcc aggcatttca gatatgcaca
      121 cagtgcatct gcctgtttca ccagcgattt ttcttcatcg ctatatgcat gctcgtcaat
      181 taacggcgca aagatatcct gcagctcttc cggaaccata tcgaccagtt tttgctgagc
      241 gattttttca atagccttgt attcctgagc gatttgcgaa ttgaagtatt tcaccggagt
      301 agggagatct ccggtgagca cttctgaagc atcgtggtac atcgccagta aagcgatacg
      361 ttcggcgttg acattaccgc caaatttacg atttttgata gctgccagcg catgggcgac
      421 catcgctacc tgcaaactgt gttcggacac attttccgtc cgcacgttgc gcattagcgg
      481 ccagcggtta atgagtttca ggcgggagag atgggcaaaa aaatggctct gtttcatagt
      541 gaccttacgt atgatgacat catcaggtca ttgtggcggg ataacactgc cggatgcaat
      601 ctccggcagt gaaattaaga ttacagcgga tgataaccgg aaaggaaacg cgcgaacttg
      661 ctcaaagaca gctcgatatc atcgacacgc ggtagcgtga caatgcggaa gtgatccggc
      721 cacggccagt tgaatgccgt cccttgcacc aacaggactt tttcctgcaa caggaaatcc
      781 agcaccattt tctgatcgtc gtgaatgtta aagcgtttgg cgtcgatttt cgggaacata
      841 tacagcgcac cacgaggttt cacgcaggaa acgcccggaa tatcgttgat caattcccac
      901 gcgcggttac gctgctcata aagacgaccg ccaggggtaa taaattcact gatgctctga
      961 taaccaccca gtgcggtctg aatggcgtgt tgcgcaggaa cgttagcaca caggcgcatt
     1021 gaagccagca tttccagacc ttcgatgtaa cctttggcgt gttttttcgg cccgttcaac
     1081 accatccacc cctgacggaa gcctgcaacg cggtacgttt tcgacagtcc gttaaaggta
     1141 atggtcagca ggtcaggtgc cagcggcgca atggagtgat gctcagcgtc gtcgtagaga
     1201 attttgtcat aaatttcatc ggcgaagata atgagattat gctgacgtgc aatctccaca
     1261 atctccatta aaagctcttt ggaatatacc gcgccggttg ggttatttgg gttgataata
     1321 acgatcccac gcgtacgagg cgtaatttta gcgcgaatat catcgaggtc tgggaaccag
     1381 tcagaggatt catcgcaaag ataatgcacc gctttaccgc tggagagcga aaccgctgcg
     1441 gtccatagtg ggtaatcagg tgcaggaacc aacatttcgt ccccgctgtt cagcaatgcc
     1501 tgcattgcct gaacgataag ctccgataca ccattgccga tgtaaatatc ttccacggta
     1561 acatcacgca tgccacgagc ctggtagtgc tgcatgatgg ctttacgcgc ggagtaaaga
     1621 cctttggaat cgcaataccc ttgagcggta ggcaggttgc gtatcacgtc aacgagaatt
     1681 tcatctggcg cgtcaaaacc gaacggggca gggttgccga tgttcagttt cagtaccttg
     1741 ttaccttctt cttccaggcg ttttgcttct ttcagcaccg gaccacggat gtcataacag
     1801 acattctcta atttgctgga tttttcaatg ggggacatga atcttgacct tttttgctgt
     1861 tatcgccact ccctgccgtg gaagtcagct tagaacaatg tactccccca ggacggtgtt
     1921 ttgaaggtct gcagaataag attttgccag aggggaaggc tcgattgtgc tgttaataac
     1981 ttaataatgg caaaataatg aggtgcatat gcttaataag tgatgttatg tagtgaattg
     2041 ttctgattct taattgtaag tgtggttttt ttatctgtaa gataattgtg tgaaaatgcc
     2101 tcgtttaaga attcactact gaatcgcgat gatgatattt ccgcattacg gagagtttaa
     2161 cagcgtgatg atttccgagg tgtcacaatg ggggcaaggc gtgaaactat tgttaagcgg
     2221 aattaatgaa tggggatgat gctaattaat tattatttaa tattcaaaaa ggttttgttg
     2281 tgcttattaa taatagaaga ttactttgcc taacataaca taactttgca tcagataatt
     2341 cgcaatgacc cttataaata aaaggttttt ggggtaaaat gcttgcataa atgacggatc
     2401 cctgctcttg atgccgcctc accattgctg tttgtacgtg ttcgcgtatg accagcgatg
     2461 ctgtacgaga gtggaacaag gactcacatt tacatttata caaacacgcg catgtcataa
     2521 atagaaaaga gtgagtaact taatagatat gcgatccctg aagatatatt ctgtgatcta
     2581 cttcaggcat tataacggaa caatgtgagt cactttttta gtgcaactca tatgcatttt
     2641 tactttcgtc cgggttccgg atgaaactgc cagggaggca cattacaaat ggaattgctt
     2701 gtttgtgtgt gcacagcatt aaccagctca gtatgagccg ccagtaagtg ataatatatg
     2761 ataagtgcaa atcgtccgat aattaacctc gacctcgatc tgctgagaac atttgttgct
     2821 gttgccgatc tgaacacttt tgctgccgca gctgccgctg tgtgtcgtac tcagtccgcc
     2881 gtaagtcagc aaatgcagcg tctggaacaa ctcgttggga aagaactgtt cgctcgtcac
     2941 ggtcgcaaca aactgttaac tgaacatggc attcaacttc ttggttacgc caggaaaatc
     3001 ctgcgtttta atgatgaggc ctgctcatca ttaatgttca gtaatcttca gggcgtgtta
     3061 actatcggtg cttcagatga atctgccgat acgatcttac ctttcctgtt aaatcgcgtg
     3121 agttcggttt atccgaaact agcgctggat gtccgcgtta aacgtaatgc ctacatggca
     3181 gaaatgctgg aatcccagga agtggatttg atggtaacca cccatcgtcc ttctaccttt
     3241 aaggcattga acctgcgtac ctcgccaaca cactggtact gcgcagcgga atacgtattg
     3301 caaaaaggtg agccgatccc tcttgtgttg ctggatgatc caagtccgtt tcgcgatatg
     3361 gtgctggcga cgcttaataa agcagatatt ccctggcgac tggcttatgt cgcatcgacg
     3421 cttccggccg ttcgtgcggc agtgaaagcc ggtcttggcg tgacggcaag gccggtcgag
     3481 atgatgagtc cggacctgcg tgtgctgagt ggagtagatg gcttaccgcc gctacctgat
     3541 accgaatatt tgctgtgcta tgacccgtcc agtaataatg agctggccca ggtgatctat
     3601 caggcgatgg agagctacca taatccgtgg caatacagcc caatgtctgc tccggaagga
     3661 gatgactcat tgttgattga aagggatatc gagtaatcga cggacgatag ataattcctg
     3721 agacaatagt gtaaaaacga accgctgggt ttattttaaa ccccagcggc ttttttttag
     3781 taaaaagcct tcctcgtatc gtgcagaacg tacaaaactg ttcatttttc aatcaccaga
     3841 gattcacgcc ccgtacgcac aaataatcac ctgaaaaaaa tctcctgcta caataatgtt
     3901 aaaaaatacg tgaatttgtt gctgcatttt tgttatggaa tgacaaaagc gtgtcacaga
     3961 tcaagaaaat actctctttt ggggggagga atcgacacaa attcctgtca aaatagaccc
     4021 gtattttttc cattgcttca caacggacac gattcaacaa catctaattt tcctagagtc
     4081 gtcaatgatc tggggagaaa gggcgccaaa tgcaaatgtt gttgtcattg tgtaaaacag
     4141 tgtgaagaaa cctttgttaa agttgacaaa aggttataga aaggagtaaa agaccacatc
     4201 aattagctgt ataaaatagt ttctgcagtt attgtaaggt tttttttatt cctccccatg
     4261 aatcgatgtg gcgtccatct gccgtaaaga gcagagaatc aggcgctact tttgatgagt
     4321 aagcaatgag tatgtcaaca tccactgaag tcatcgctca tcactgggca ttcgctatct
     4381 ttcttatcgt tgccattggc ctgtgttgcc tgatgctggt aggcggttgg tttttaggcg
     4441 gtcgcgcacg cgcgaggtca aaaaacgttc cgtttgaatc cggtatcgac tcggtcggct
     4501 ccgcccgctt acgcctgtcc gccaagtttt atctggtggc catgttcttc gttatcttcg
     4561 acgttgaagc gctgtatctg ttcgcatggt caacctctat ccgcgaaagc ggctgggtag
     4621 gctttgtgga agctgcaatt tttatttttg tgttactggc aggtctggtt tatctggtgc
     4681 gtattggcgc gctggactgg acgcccgcgc gttcacgccg cgagcgtatg aacccggaaa
     4741 cgaacagtat cgctaatcgt caacgctaac cgcgaggcat taagatggat tatacgctca
     4801 cccgcataga tcccaacggt gagaatgacc gttaccccct gcaaaagcag gagatcgtaa
     4861 ccgcccctct ggagcaagaa gttaacaaaa acgtgtttat gggcaagctc aatgacatgg
     4921 ttaactgggg tcgtaaaaac tcaatttggc cgtataactt cggtctttcc tgctgttacg
     4981 ttgagatggt gacttcgttt accgcggtgc atgacgtggc gcgttttggc gctgaagtat
     5041 tgcgtgcttc gccgcgtcag gctgacctga tggtggttgc aggaacctgc tttaccaaaa
     5101 tggcaccggt tattcagcgt ttgtatgacc agatgctgga accaaaatgg gttatctcaa
     5161 tgggtgcctg tgccaactcc ggcggtatgt acgatattta ttccgttgtg cagggcgttg
     5221 ataaattcat cccggttgat gtgtatatcc cgggctgtcc gccgcgtcct gaagcgtaca
     5281 tgcaggcact gatgctgttg caggaatcta tcggtaaaga acgtcgtccg ctctcctggg
     5341 tggttggcga tcagggcgtt tatcgcgcca atatgcaatc agagcgcgaa cgcaagcgcg
     5401 gtgaacgcat tgccgttacc aacctgcgta cacctgacga gatttaattt gcgcctgtcg
     5461 gcaaagggaa ttttcttcgc ttattcctaa atctatttcg cgaagcttac tgcgccgaca
     5521 gtcaccacgg accatttgca atggtgaaca atatgaccga cttaaccgcg caagaacccg
     5581 cctggcagac ccgcgatcat cttgatgatc cggtgattgg cgaactgcgc aaccgttttg
     5641 ggccggatgc ctttactgtt caggcgactc gcaccggggt tcccgttgtg tggatcaagc
     5701 gtgaacaatt actggaagtg ggcgatttct taaagaaact gccgaaacct tacgtcatgc
     5761 tgtttgactt acacggcatg gacgaacgtc tgcgcacaca ccgcgaaggg ttacctgccg
     5821 cggatttttc cgttttctac catctgattt ctatcgatcg taaccgcgac atcatgctga
     5881 aggtggcgct ggcagaaaac gacctgcacg taccgacctt taccaaatta ttcccgaacg
     5941 ctaactggta tgagcgtgaa acctgggatc tatttggcat tacattcgac ggtcacccga
     6001 acctgcgccg catcatgatg ccgcaaacct ggaaaggtca cccgctgcgt aaagattacc
     6061 cggcgcgcgc taccgaattc tcgccgtttg agctgaccaa agccaaacag gatctggaga
     6121 tggaggccct gaccttcaaa ccggaagagt gggggatgaa gcgcggcacc gaaaacgagg
     6181 acttcatgtt cctcaacctc ggtccgaacc acccgtctgc gcacggtgct ttccgtatcg
     6241 ttttgcagct tgatggcgaa gagattgtcg actgcgtacc agacatcggt taccaccacc
     6301 gtggcgcaga gaaaatgggc gaacgccagt cctggcacag ctacattccg tataccgacc
     6361 gtatcgaata cctcggcggc tgcgttaacg aaatgcctta tgtgctggcg gtagagaaac
     6421 tggctgggat caccgtgccg gatcgcgtta acgtcattcg cgtaatgctc tccgaactgt
     6481 tccgcatcaa cagtcacctg ctgtatatct ccacctttat tcaggacgtc ggcgcgatga
     6541 cgccagtgtt cttcgccttt accgatcgtc agaaaattta cgatctggtg gaagcaatca
     6601 ctggttttcg tatgcacccg gcgtggttcc gtattggcgg cgtagcgcac gacctgccgc
     6661 gcggctggga tcgcctgctg cgtgagttcc tcgactggat gccgaaacgt ctggcgtctt
     6721 acgagaaagc ggcgctgcaa aacaccattc tgaaaggtcg ttcccagggc gttgccgctt
     6781 acggcgcgaa agaggcgctg gagtggggca ccaccggcgc gggcctgcgt gccaccggga
     6841 tcgacttcga cgtgcgtaag gcgcgtcctt attctggcta tgaaaacttc gacttcgaaa
     6901 ttccggtggg cggtggtgtt tctgactgct acacccgcgt aatgctgaaa gtagaagagt
     6961 tgcgccagag tctgcgtatt cttgagcagt gcctcaacaa catgccggaa ggcccgttca
     7021 aagcggatca cccgctgacc acgccgccgc cgaaagagcg cacgctgcaa catatcgaaa
     7081 ccctgatcac ccacttcctg caagtgtcgt ggggtccggt gatgcctgcc aatgaatcct
     7141 tccagatggt tgaggcgacc aaggggatca acagttacta cctgaccagc gacggcagca
     7201 ccatgagtta tcgcacccgt atccgtacgc cgagttatgc gcatttgcag caaattccgg
     7261 cggcgatccg cggcagcctg gtgtctgacc tgattgttta tctgggcagt atcgattttg
     7321 ttatgtcaga tgtggaccgc taattatgca cgagaatcaa caaccacaaa ccgaggcttt
     7381 tgagctgagt gcggcagagc gtgaagcgat tgagcacgag atgcaccact acgaagaccc
     7441 gcgtgcggcg tccattgaag cgctgaaaat cgttcagaag cagcgtggct gggtgccgga
     7501 tggcgcgatc cacgcgatcg ccgatgtgct gggtattccg gcgagcgacg tcgaaggcgt
     7561 ggcaacgttt tatagccaga tcttccgtca gccagtaggc cgccatgtga tccgttattg
     7621 cgacagcgtg gtctgtcaca tcaacggtta ccagggtatt caggcggcgc tggagaaaaa
     7681 actgaacatc aaaccagggc aaacgacatt tgacggtcgc ttcacgctgc tgccaacttg
     7741 ctgcctgggg aactgtgata aagggccaaa catgatgatc gatgaggaca ctcacgcgca
     7801 tctgaccccg gaagcgatcc ctgaactgct ggagcggtat aaatgaaaaa cattatccgt
     7861 actcccgaaa cgcatccgct gacctggcgt ctgcgcgatg acaaacagcc agtgtggctg
     7921 gacgaatatc gcagcaaaaa cggttacgaa ggcgcgcgta aggcgctgac cgggttgtct
     7981 ccggacgaaa tcgttaatca ggtaaaagac tctggtctga aagggcgcgg tggcgcaggc
     8041 ttctcgactg gcctgaagtg gagcctgatg ccgaaagacg aatccatgaa tatccgttac
     8101 ctactgtgta acgccgatga aatggagccg ggcacctata aagaccgtct gctaatggag
     8161 caactgccgc acctgctggt ggaaggcatg ctcatctccg cgtttgcgct gaaagcttac
     8221 cgtggctata tcttcctgcg cggcgaatat atcgaagcgg cagtgaatct gcgtcgtgcc
     8281 attgccgaag ccaccgaagc gggtctgctt ggcaaaaaca ttatgggaac aggtttcgat
     8341 ttcgaactgt tcgtccat
//
LOCUS       DAWDRC010000102         8323 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4178_length_8323_cov_2.222908, whole genome shotgun sequence.
ACCESSION   DAWDRC010000102 DAWDRC010000000
VERSION     DAWDRC010000102.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4178_length_8323_cov_2.222908"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    353..362
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2542..2551
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3157..3166
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5489..5498
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6042..6084
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2029 a         2103 c         1949 g         2159 t
ORIGIN      
        1 tatgggtgta tgattttttt gattatattg ataaaatgtt tttctgatgt tttatcttct
       61 ccaaaatcag cactcccata ataaacagca ttaatctgcg tcttcattaa taaataccca
      121 cgaaacttct taaacagttt catatccggt ttaaaatcgg cctgccagaa ctggtgcaaa
      181 tgcccttgat acgtcaagcc tttgatgtcg tacagggcat tgcccatcac tttgagtggt
      241 ttgttatgaa tcagtgcaga gatccccgcc gtactgttaa tcgtcaccac cgcttttgcg
      301 tggcgtaata attccggcat cggcagatcg tgcacatata tgactcgctc acnnnnnnnn
      361 nngctcacct aagccatatt ccttactcaa tcgcttaatt aatggtcgat agagtctgtg
      421 accacgatcc atcggatgat gtttgatcac caaataactc tctttcggcg ctttacgcga
      481 aaatgagtac atgacttcat taatatagtc acgcacatcg ttataattgc tgtggttacg
      541 aatctggcta tcgttatata cctgcaaaac ggcaagataa taacgctggt ccaactcgtt
      601 cattaacctc ggtaatacct tacgctgtgt taccttgtaa agttgcttgc gccagtatgc
      661 acgaacccag caacgtgctt cataccaggg ggaaaacgat ttatggtggc ggtagcgagg
      721 gaactcatgg cggtaatgcc agcccatcag gtaataccac atcgcatgac ctatacgttt
      781 catcgttgaa ggttttaagt tctcaacttg cggcgtaggc atatctggta acttacgata
      841 aaaatccgga tcgcgcggta gcgatgaata tgcgttcact ccgccttctt caacggtaat
      901 aaattgcgga cgtaaatacc cctcttcaaa tgccagaaag cggatctctt tcgtattcgc
      961 ccaccgtttt gcttctttgt gtaatgggcg gcagtcgcca aagcagagga tggtatcaaa
     1021 gtcatattgt cggtggagat ctcgcaacca accgggaaac tctttcggcg tttggtagta
     1081 agccaggtat tgtcgattgc ggcagtaaaa acgatctccg ccgttgaata caacattcac
     1141 aacgttacgg cctaatgact ctaaccattc cgcgatgtca ttgaaaaaag gtcccattgg
     1201 tccctgcaat agcagatatt ttttaccgga taataagacg gttagtgcat tatcttgcat
     1261 aaataatttg cgtaatagtc aacaatgaaa ttgtttagcc cagtcttact ttgcagaaca
     1321 tgattaactt acggtaaatg ttaataactc tgtaaatatt attttttcta ataaacatcg
     1381 gcttgcgcgg agtctggatc aaatattccg ccgcctcttc aaccgtaata ggttgtagcc
     1441 gtgttgggtg gatataggtt ggataaacaa tcagcgcctg atagatcaaa tccgctaacg
     1501 ttaattttcg ctcgcggcgc gggcagcgat gttcatcaac ggttaaaccc caaccggcat
     1561 agaagggcag gccgtaacaa tgtacgcgct tgccatgtaa taatgcttca aaccccgaca
     1621 gcgacgtcat ggtatgcact tcatctgcgc gttggatgca ttgaataata tctgcgtcca
     1681 gtgcctgata atcagcaagt tcagcaatct gttctgccgg aatattgccc ttgcgattcc
     1741 ccaccagtac atccgggtgc ggtttataaa taatgtaagc gtgcggattg cgctcgcgta
     1801 ctgtgcgtaa taactcaagg ttgctcttaa tcgagacagt gcctgtttta attgaggcat
     1861 cgtcttctac ctgacccggc accaggataa cttttttatc tttggcttcg gctggtagag
     1921 agaaatcggc tcccaggttg tacttactca gtttgctttc aaccagtcgc tggcgtaatt
     1981 tttcagctcg catcttctgc gccagcgtta gctggctatg attcagcagc acttccagat
     2041 cgctggggcg cgtggcgtca tagtaaatac cgcgtttatc cagcaccagc gatagcggcg
     2101 gcagcaggtc agagccaagt ccggatgaac gcagaaaacc atcttccatt cgccataacg
     2161 gcagtgattt tcgctgcgct tcggctcgcc attgctgctc tccctttaca ccccatacca
     2221 cgcaagccgt cgccgcagta caacggcgtg aataactcag tctgtttgtt gcggtacgca
     2281 agaaaggttt caggatcgct gacttccaca gcgttaagcc tggcgcccat aaatagccat
     2341 tacgctgttg ctgatggttg cgctgtaatt gcagccattg cagtacggta aacagatcgc
     2401 ttgcggcccc cgtgagcggg tcgatatagc gacagtaacg caggtatgca gcggaaaaaa
     2461 gttcttccag cgtggcagaa ccgcgtcggg cagataacaa agcggactgc ggatggcgat
     2521 cgtcggttaa gccccagcct gnnnnnnnnn naaacatgtt actggttttc ctgccagcaa
     2581 ggcttcaaag ccgtattggg atgtcacgac gtaaacccgg gaaacgtgtc gcaatagcga
     2641 ctgcgggctg acattctcgg caatcaaacg tactcgttgc gtggcacgca gatcagcgaa
     2701 ataacctgtt ttctttcctt ccaggacatc cggatgcacc ttcacccaaa tttcggcttg
     2761 aggattttcc gccatcgcag cttccagcat ggcagcaaac tcatgcgggc cagcattgcc
     2821 atacgtcacg gacatgtcat taaatgtctg atcgacaacc agaacgatgt ctgcacgttc
     2881 tgactcatca gccacaaatg caggcgccaa gttatatttc gacaaatccc cggtcacgat
     2941 ggtgtgcatc gcttctctgg cctggcttat cagagctgta tttccggctt tatcctgtac
     3001 cagtttctcc aatgctgaag gcttgctggc atcgtagtaa atgccacaat catccaccac
     3061 cagagaaagc ggcggcgcgc cattgacgcc aagatccagc gaacgcacaa atccatcttc
     3121 cagacggatg atgggttttc ccgctgcatt agcgatnnnn nnnnnntgct ttagcgatgg
     3181 cggctggttt cgccgcgctg gggcgatgtc cccacacggc gatagcatcg acgttttgcg
     3241 gaacaggacg cagcagagaa agtttctggc acggttgtgc cagaaatttc tccagatgcg
     3301 gaatacgcca gatgccaggc gagtaaatgc caatcatgcg ttttcagcca gttcttgcgc
     3361 catcagggca cgcacctttt caaggcatgc tggggtgtcg acgcccggac cggttgcggc
     3421 aacctcaaat gtacggatgt tgatccccgc gttcattaac cgtagctgct ccagttcctc
     3481 gatttgactt aatgccgtcc ttggtaactg actgtagttt tgcagcacat cgcgacgata
     3541 agcgtaaata ccaacgtgtt tcaggtagcg cgctttttca gcattgcgcg gatagggaat
     3601 cggcgaacgg ctgaaataaa gtgcatcctg gcgggtattt accaccactt ttaccgtgct
     3661 tggctcagcc gcttcttcgg cagaaatcgc gtggcatagc gttgccactg gcaacgcggg
     3721 gtcatcacgc attccttgta acagcgtttc tacatcccgc gggcgaatca ttggttcgtc
     3781 gccctgcagg ttaatgtaga tatctgcctc gactttatgt atcacctcga ccagccgatc
     3841 ggtgccggat tcatgatcgt tgcgcgtcat gatggctttc ccgccaaatg cctgcacggc
     3901 cttttcaaca cgctgatcgt ctgttgccac ccaaacttcc gcaacgcccg ccacctgtaa
     3961 cgcacgctcg taaacatgct ggatcatcgg tttaccaaca atatcgagca gtggcttacc
     4021 cggcaggcgc gatgagccat aacgagccgg gatgacaata acagctttgc tcatttcaac
     4081 tccttacaaa gacagaatca cttttgcacc caccgccagc tgatagagga tggtggaaat
     4141 accacgggta acttcaatat ttttcgattc atatttcggc agaaccataa tctcatcgcc
     4201 cggtttgagt gaatccacgt cttccgcgtt gacggcagca ccgttctgac gaatgacgat
     4261 aattctggcg ttacccgatt tctgcgtcag gccaccacat ttctcgatgt aatcctcggt
     4321 ggtcataccc ttctgccagc tcaccgcgtt cgggaacagc acctcgccat gaaccataac
     4381 cagcgatgtt ttctccggaa tattgatgac gtcgccatct tcaagcagaa cagaatcaat
     4441 attggattcg ttgaggatca cttcaccttt cggaacaacg gtgcgtgctt tcgccacaaa
     4501 gcggctgatc agttgtgctt cctgcattcg caggctggct tcttctttgg tggatgactg
     4561 ggcagaaagt gatgcttcct ccagcttttg cagcgagaga ttcagcatct ctttctgacg
     4621 ctgagccact gatgggcgat aaagctgaac tgcgttcatc tgcgacatgc tgttcgggcg
     4681 gactttttcc agaaccgcac gcatagtgga accataaggc agtaccatgg catgttcacc
     4741 ggagtgtgcg ccttcaaccc gcacctgaat ggtgccggca tagcggtcag tgctcacgat
     4801 taaggtatcg ccattttgca acatacggcc tggcgcagaa ctgatcggat agtattcgct
     4861 gcgtttttgc agcccctgtt tacgcataat cgtaatgtga gtcgcgccag gcttagggcg
     4921 cgcccagctc aacgcttccg ttacgggaat gctgctttcg cggaactcaa agtcgtagct
     4981 gttaaagaca tcgccctgaa cgctgaaagt atgctgacgc ggcccgacga tgatggtgtc
     5041 accatcggcg aactgcgaaa gccccagttt gccgttcagc aggaagtcgt acaggttgac
     5101 gttggagcgc acgcggttac cgcgcttgac cacaatatca acgtaacttc cgcgctctgg
     5161 atcaacgccg ccagccttga tcagataatt gagtaacgaa tcagacgtca caccgccata
     5221 cagaccagga ttacgcacaa atccggtcac gtacactttt actggctgcg cctgtaataa
     5281 ggaggcgtag acgttgacgt tggactggta tacttccttc actttggatg tgaccagggc
     5341 atttagctga ctattactga cgccagcaac tttcaccgga ccaacgttcg gcaggaaaat
     5401 attacctttg ggatcaacct gtaacgcacc atcaaaggtg aacgcacccc acaagcgaac
     5461 ctgaatgcta tcacccggat tcagaatann nnnnnnnnca tacggctcat taccaccggc
     5521 ggtgagggcg gcggggtatt gtcgaagccg ctcatgtttt gcgtatccga ctgttgcccg
     5581 ttcagaatac cggtaagcgg cgcggctcct gtaaggtttg ggtcagcgtt aatatcaatg
     5641 gccgcgctgg cctgcgccgc gtgacaggcg gcaatcagta aaattgattt aaataatttc
     5701 atcacaaact cattcagcga cagcgttagt ctcggtgatc ttcaataaca gccagcaaca
     5761 gtttcagggt gccgaacagc aggcagcaca ccagtaacca gcaggcgatc aaataaggga
     5821 tatttggaaa agacgattcc tgcggcaact gtggcgaact gatcactgac agcaccttga
     5881 gcttacgagc cgcttctaca cgggtctttt caatggaggt cagggtcagt ttgtacagct
     5941 cggtgttgaa ctctactttt gatttgattt cttcaaaatc cactgccata cggtttagct
     6001 tgtcaccctg tggcgcagtg attttgcttt gttcttcatc gnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnntgcctg catgactgaa tcgcattacg cgcactcact
     6121 acttgcgggg cgtcctcacg cagatacgtc agcaagttac gcagatccgc ttccatctgg
     6181 atcttctggc ccatcagcgt attgactaac gtgctcgccg cctgtgcctg tgcctgtgga
     6241 tccagaacgt tgttgttgtc ctgataagag agcaattccg ctttgctggc gtccagacgc
     6301 tggcgtgcct tttccatctc cgtttctgca aaggcaagct ggtcacgcgc gatgcgatgt
     6361 gacatctcat tgataaagcg ctctgactct ttcagcacgg tctggttaaa cttaagcgca
     6421 aactccgggc taaagccctg cgtctgaata ttcagtaatc cggttttatc gtcatacgag
     6481 acgttgatac ggtccttgta gtacttcagg aatccttctg cggtttcatc cttgctaaga
     6541 tgattaagaa aatcgagccc gctgtggcta aatgcttcac gaaaatttag ttgcttatcc
     6601 agcgccgcca gcatatccgg cgagttgatg tactctttca gatacaaagc atcttctgcg
     6661 gaactggggt tagaggcacc cagaagcaga ccaaaattca ggctgccgct gtttaaatca
     6721 tccgagcgtt taatggcgac tttcgactcg ctcatatagc gaggctggct gaagatagcc
     6781 agatagatca gcagcaccgc catcggtgcc agaatgatga ttttcgccag gtgtttttgg
     6841 atatctgcca gtgagatggc agacagacga gcacgcatcc aggatacggc agacttcact
     6901 tttatcaaca tgtaacgatt atcaaagttt cagtggggcg caaactcaat gagagactaa
     6961 cctctcgaac cccgttgctt aattagtcga aaatgcgcac aaggccagtg actttatttg
     7021 ccttgttggt caccaataac gttgagacac ggtgtttttg cattttttct tccgcttcaa
     7081 tgatcatggt gtcttccggt agcgtcagcg gttcacgtgt catcatctgc gcagccgtgg
     7141 cggatgtcag agaatcctct ttttccataa agcgacgcag gtcaccgtcg gtgataatgc
     7201 ccgctagccc accttctgcg tcttctacca tcaccattcc ctggcatccg ctggtgatac
     7261 gttgaatcac ggttttaaat gacgcatcca gctgtaccgc aggaacatca tgctgcatga
     7321 catcagcaac gcgggtcagc aggcgacgac ctaatgaacc gcccgggtga tagcgcgcaa
     7381 aatcattcgg cataaatttg cgttggcgga tcatggcaat cgccagcgca tcgccgatcg
     7441 ccatcgtcag cgtggtagac gttgttggtg caagattatt cgggcaggtt tcattcgcca
     7501 tgtggagttc cagcacggca tcagcatttt tcgccagcgt ggaatttcca ttattggtaa
     7561 tggcgattat tcggttgccg aaatttttca gtgaaggaac tagcttgagg atttcatccg
     7621 tttcaccgct ggcagaaata aggatcagaa gatcgtaagg cgtaatcatg cccagatcgc
     7681 catggaaagc ttctgcagga tgaataaaga aactaggcgt acccgtagag gccagcgtcg
     7741 ctgacatttt acgaccgaca tgccctgatt tacccattcc cgaaagaata acgtgccctt
     7801 tacagttcat tatcaaatta aggacacgct ggtactgccc ggaatccagt tgtttagaca
     7861 agttttgtaa tgctgcgcct tgttctgcca gagtctggcg aacagaggta attagatatg
     7921 gatcgatagt actgctctgg tcatcaggta aatgtctttc agacatatca gctcctttgc
     7981 acggaaataa tgccacagtc acaacaaacc cgaacccact ataatttatt tatgctaaat
     8041 caatgggttg taacaaaatt tttaatgaat ataaaaccat ggtcagtccc tgcttatatg
     8101 caggacttat caccttctta ttaagtcgca atgtctcatg gaggtgcgaa gatgctgcaa
     8161 tattacaggc gagaagggga accaaatttt gcccacctat ttaacacacc cctacattat
     8221 gaacaaggaa tttaggattt ttatcattta ttaaaatgtt tctcacttga ctattaagac
     8281 caaaaacaat ttatcaattg attatttcct cgtaaaaaaa gaa
//
LOCUS       DAWDRC010000103         8299 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4187_length_8299_cov_2.581878, whole genome shotgun sequence.
ACCESSION   DAWDRC010000103 DAWDRC010000000
VERSION     DAWDRC010000103.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4187_length_8299_cov_2.581878"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    796..805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2052 a         1981 c         2071 g         2185 t
ORIGIN      
        1 ctgcataaat atgaatatat ttatttattt ttcaatgtat taagctattt tcgttgcaat
       61 ttttgaccca tgattttctt ttcgaattgt cgatatgatt gaaggataac tttcaaagaa
      121 aaatacaagg ggctatagtg ttacgcatta aaggccaatg aagagtgtta acgtgccgag
      181 aaaatcgtct aacctactga aacgcagatt aaaaattgca gaaattgttg ccgcccaggg
      241 tgaaatcaaa gttgatgact tatctgcgca acttggtgtc tctggcgtga cgatccgcgg
      301 cgatctcagt tatctggagc aacagggata tttaaagcga tcatttggcg gtgctattgc
      361 gacaccactc tcctctgaac cagaagcgat acaaccgatc gcgccattgt ctctggcaca
      421 acaaatggaa atagcacgac attgcgcacg attaattcgc gatcgtgaca cgctatttct
      481 tggacatggg acaatctgcc gtaagatcat tccattgctc agcgaagtga aaaaactgcg
      541 tctgatcacc aacgatcccg aacatgcgct gctggcaaat cagtttattg atggcgaaat
      601 aatactggct ggcagtgaaa tgctgcgccc cgacaccgca ctggccggaa aacagcttga
      661 acaggcactt cagcatttca ccattacaca tagcattctg gaagtcagcc atctggatgc
      721 tgatggtact ttaaatatca acgttcccag actcgccgct gcctggcagc tctgtttcga
      781 tcatgcccag aataannnnn nnnnntttcg ttgccgccga tcctgcgcca tcaacggctg
      841 ctatcgggca tttgcatcag gcagaaaatg tgatcgtcag ccgcagcatt aacgaacaat
      901 atcagcaaca attgcagtcg gctgatttcg ttatccttta taccagtaat gagtgcttta
      961 cctggtccaa tcgcgaaagg cttcaggaat gagaggggtt ttgtgacaat aactatctgc
     1021 gctatgggcg aattgctggc cgagtttttg tcccgcaacc cacatcaaaa attcactcag
     1081 cctggggagt ttatcgggcc atttcccagc ggtgcgccag caatttttgc tgctcaggtg
     1141 gcaaaactgt cccatcgggc catcttcttt ggatgtgttg gtaatgatga ttttgcccga
     1201 ctcattatag agcgtctccg tcatgaaggt gtcattaccg atgggatcca tgttatgaac
     1261 aatgccgtca caggtacggc gttcgtgagt tatcaaaatc cccagctgcg ggatttcgtc
     1321 tttaatatcc ctaacagcgc ctgcggtttg tttactgccg agcacattga taaggatctg
     1381 cttaaacagt gtaaccatct gcatattgtg ggctcatcgt tgttctcatt tcgcatgatc
     1441 gatgtcatgc gtaaagcaat aacgacgatc aaatcggctg gcggcaccgt ttctttcgat
     1501 cccaatattc gcaaagagat gctgagcatt cctgaaatgg cgcaggcact cgattatttg
     1561 attgaatata cggatatttt tatccccagc gaaagcgaac tccctttctt cgcgcgtcac
     1621 aaaaatctgt cagaggaaca gattgttagc gatcttctcc acggcggcgt aaaacatgtg
     1681 gcgataaaac gcgcccagcg tggggccagc tattacaagc ttaaaaacgg tacattacac
     1741 gcccagcatg ttgcaagtca cgatatcgaa attatcgatc caacgggtgc aggcgactgc
     1801 tttggcgcaa cgtttatcac tcttttctta tccggtttcc cggcacacaa ggcgctgcaa
     1861 tatgcaaatg ccagcggcgc gctcgccgta atgcggcaag gtccgatgga agggatatcc
     1921 tcactggcag acattgaaga ctttttgcag cagcactgaa cggcaatttt ctgtcagatc
     1981 attttccctg cctgaagact tgccgtagaa ttgcccgttt ctcgtatccc cccatgagga
     2041 ggaccccttc cgtggacact cgttttgttc aggcccataa agaggcgcgc tgggcgctgg
     2101 ggctgaccct tttgtatctg gcggtatggt tagtatccgc ttacttacct ggcgttgccc
     2161 ccggttttac cggctttccg cgctggtttg agatggcctg catcttgacg ccgctgctgt
     2221 ttattggact gtgctgggcg atggtgaaat ttatctatcg cgatatccca ctggaggatg
     2281 acgatgcagc ttgaagtaat tctaccgctg gtcgcctatc tggtggtggt gttcggtatc
     2341 tctgtttatg cgatgcgtaa acggagcact ggcaccttcc ttaatgagta tttcctcggc
     2401 agccgctcta tgggcggtat tgtgctggcg atgacgctca ccgcgaccta tatcagtgcc
     2461 agttcgttta tcggcgggcc tggagctgct tataaatacg gcctgggctg ggtattgctg
     2521 gcgatgattc agcttcctgc agtctggctt tcgctcggta ttctcggcaa gaagttcgca
     2581 attcttgcgc gccgctacaa tgcggtgacg ctgaacgata tgctgtttgc ccgctaccag
     2641 agtcgtcttc tggtatggct ggcgagtttg agtttgctgg ttgcgttcgt tggtgcgatg
     2701 accgtgcagt ttatcggcgg tgcgcgcctg ctggaaaccg cggcgggtat tccttatgaa
     2761 accgggctgc tgattttcgg tatcagcatt gcgttgtata ccgcctttgg cggctttcgc
     2821 gccagcgtgc tgaacgaacc cttgcaaggg cgggggaggc tgtttggcac cgttgtgctg
     2881 cttattggcg tggtccatgc tgctggcggc ttaagcaatg cggtagagac gctgcaaacc
     2941 atcgatccgc agctggttac gccacaaggc gctgacgata ttctgtcgcc tgcctttatg
     3001 acgtcgttct gggtgctggt gtgttttggc gtgattggcc tgccgcatac tgcggtgcgc
     3061 tgtatctctt ataaagacag caaagccgta catcggggga tcatcatcgg tacgattgtg
     3121 gtcgcaatcc tgatgttcgg tatgcatctg gccggagcgt taggtcgggc ggtgatcccc
     3181 gatctcaccg taccggatct ggtgatcccg acgttaatgg taaaagtgct gccaccgttt
     3241 gccgccggga tcttcctggc tgcaccgatg gctgcgatca tgtcgacaat taacgcccaa
     3301 ctgctgcaaa gttccgctac gatcattaaa gatctctatc tgaatatccg tccggatcaa
     3361 atgcaaaacg agacgcgtct gaagcggatg tcggcggtga tcacgttggt tctcggtgcg
     3421 ttgctgctgc ttgccgcctg gaagccgcca gaaatgatca tctggctgaa tttgctggcc
     3481 ttcggtgggc tggaagccgt tttcctgtgg ccgctggtgc tgggtcttta ctgggaacgt
     3541 gccaacgcca aaggcgcgct aagtgcgatg atcgttggcg gcgtgctgta tgccgtactc
     3601 gcgaagctga atattcagta cctgggcttc caccctatcg tgccctcgtt actactaagt
     3661 ttgctggctt tcctggtcgg aaaccgtttc ggtacatccg tcccgcaagc taccgttttg
     3721 actactgata aataaagagt tttgccatgc cttggatcca actgaaactg aacactaccg
     3781 gcgcgaacgc ggaagatctt agcgatgcgc tgatggaagc gggtgccgtt tctatcactt
     3841 ttcaggatac ccacgatacg ccagtatttg agccgctgcc gggcgaaacg cgcctgtggg
     3901 gcgacaccga tgtgattggt ctgttcgacg ctgaaaccga tatgaacgac gtggtggcga
     3961 ttctggaaaa ccatccgctg ctcggcgcgg gcttcacgca taaaatcgaa caactggaag
     4021 ataaagactg ggagcgcgaa tggatggata atttccaccc gatgcgcttt ggtgaacgac
     4081 tgtggatctg cccaagctgg cgtgatgtgc cggacgaaaa cgccgtcaac gtgatgttag
     4141 acccagggct ggcgtttggt acgggtaccc atccaactac ctctctgtgc ctgcaatggc
     4201 tcgacagcct cgatttaacc ggtaaaacag tcatcgactt tggctgcggt tccggcattc
     4261 tggctatcgc ggcgctgaaa ctgggtgcag caaaagccat tggtattgat attgatccgc
     4321 aggcgattca ggccagccgc gacaacgctg aacgtaatgg cgtttctgat cgtctggaac
     4381 tctacttacc gaaagatcag ccagaagaaa tgaaagccga cgtggtggtc gctaccatcc
     4441 ttgcgggtcc attacgtgaa ctggcaccgt taatcagcgt cctgccagtt tcaggcggtt
     4501 tgctgggcct ttccggtatt ctggcaagcc aggcagagag cgtttgcgaa gcttatgccg
     4561 acagcttcgc tctggacccg gtcgtggaaa aagaagagtg gtgccgcatt accggtcgta
     4621 agaattaacc ttcgcatcgc cgtagggtga cgcaaggcga agtgcgagca aggtcacaaa
     4681 aggcacgtta atttgccgat tatttacgca aatttgcgtg ccaaaatttt cattcataaa
     4741 gaaaaattga gaacttactc aaatttcttg gagtgtaaat tttagtcact attttctaat
     4801 ataatgattt ttatgagtaa ttatcgcgcc aagctcattt taaatgcaat tctttgatcc
     4861 atctcagatg attggtcaaa gtttggcctt tcatctcgtg caaaaaatgc gtaatatacg
     4921 ccgccttgca gtcacagtat ggtcatttct taactcatgc gcatcggaca atatcagctc
     4981 agaaatcgcc tgatcgcagc gcccatggct ggcattacag acagaccttt tcggacgttg
     5041 tgctacgaga tgggagccgg attgacagta tccgagatga tgtcttctaa cccacaggtt
     5101 tgggaaagcg acaaatctcg tttacggatg gtgcacattg atgaacccgg tattcgcacc
     5161 gtgcaaattg ctggtagcga tccgaaagaa atggcagatg cagcacgtat taacgtggaa
     5221 agcggtgccc agattattga tatcaatatg ggttgcccgg ctaaaaaagt gaatcgcaag
     5281 ctcgcaggtt cagccctctt gcagtacccg gatgtcgtta aatcgatcct taccgaggtc
     5341 gtcaatgcag tggacgttcc tgttaccctg aaaattcgca ctggctgggc accggaacac
     5401 cgtaactgcg aagagattgc ccaactggct gaagactgtg gcattcaggc tctgaccatt
     5461 catggccgta cacgcgcctg tttgttcaat ggagaagctg agtacgacag tattcgggca
     5521 gttaagcaga aagtttccat tccggttatc gcgaatggtg acattactga cccgcttaaa
     5581 gccagagctg tgctcgacta tacaggggcg gatgccctga tgataggccg cgcagctcag
     5641 ggaagaccct ggatctttcg ggaaatccag cattatctgg acactgggga gttgctgccc
     5701 ccgctgcctt tggcagaggt taagcgcttg ctttgcgtgc acgttcggga actgcatgac
     5761 ttttatggtc cggcaaaagg gtaccgaatt gcacgtaaac acgtttcctg gtatctccag
     5821 gaacacgctc caaatgacca gtttcggcgc acattcaacg ccattgagga tgccagcgaa
     5881 cagctggagg cgttggaggc atacttcgaa aattttgcgt aaacagaaat aaagagctga
     5941 cagaactatg ttcgaacaac gcgtaaattc tgacgtactg accgtttcta ccgttaactc
     6001 tcaggatcag gtaacccaaa aacccctgcg tgactcggtt aaacaggcac tgaagaacta
     6061 ttttgctcaa ctgaatggtc aggatgtgaa tgacctctat gagctggtac tggctgaagt
     6121 agaacagccc ctgttggaca tggtgatgca atacacccgt ggtaaccaga cccgtgctgc
     6181 tctgatgatg ggcatcaacc gtggtacgct gcgtaaaaaa ttgaaaaaat acggcatgaa
     6241 ctaattcagg ttagctaatt gcttgattaa aaggcgctac tcggcatggg gaagcgcctt
     6301 ttttataggt gtcacaaagg gagtgaccat gagaacagga tgtgaaccga cccgatttgg
     6361 taatgaagca aagaccatca tccagggtga tgcattaacc gaacttaaaa agctccccgc
     6421 cgaaagtgtc gatctgatct ttgccgaccc accgtataac atcggtaaaa attttgatgg
     6481 tctgatcgaa gcctggaaag aagatctgtt tatcgactgg ctgtttgaag tgattgcaga
     6541 gtgccatcgc gttctgaaaa agcagggcag catgtacatc atgaatagca cggaaaacat
     6601 gccctttatc gatctccagt gccgcaagct ttttaccatc aaaagtcgca tcgtctggtc
     6661 atatgacagt tctggagtgc aggcgaaaaa acactacggc tccatgtacg aacccatcct
     6721 gatgatggtg aaagacgcaa agaactacac gttcaacggt gatgctattc tggtagaagc
     6781 caaaaccgga tcgcagcgcg cgttgatcaa ttatcgcaaa aatcctccac agccttacaa
     6841 tcatcaaaaa gtaccgggta acgtctggga ttttccgcgc gtgcgttatt tgatggatga
     6901 atatgaaaac cacccgacgc aaaaaccgga agccttgctg aaacgcatta ttctcgcctc
     6961 ttccaaccca ggcgatatcg ttctcgaccc gtttgccggt agctttacta ccggtgccgt
     7021 agccgtcgcc agcgggcgaa aattcattgg tattgagatc aacagcgagt acatcaaaat
     7081 ggggcttcga cggctggatg tcgcgtcgca ttactctgcg gaagaactgg cgaaagtgaa
     7141 aaaaagaaag accggcaacc tgtcaaaacg aagccggtta agcgaagttg accccgatct
     7201 cattgcaaag taaaggaagt gtaagcctat ttttcagatt attcatttcg tgtatatttc
     7261 ctgccagact tggttaaaca tgcacaggct caggtaatga ttcgcaagta ttggtggctc
     7321 gtcgttttcg ctgtcttcgt ttttctgttt gatactttac tgatgcagtg aattgaactg
     7381 ctggcaacag aaacagacaa atgccgcaat atgaactcag ttaatccact aaaactggtt
     7441 aactttgacg aactgaattt tcaggacaga atgtgaattt atatgacact taattcattc
     7501 gttttatgaa ttaatttcgt tatgttttca tctggcatga acattcttaa tacgttatcg
     7561 accagagcgg gggcttgttt ataaagatca taactcgcca tattcattaa ccagttttga
     7621 acaattccgc tgaaggcacc atcaataata atcatcacaa catctaaatc gagattattt
     7681 gctacacaac cttgttgctg acacgcctgc aatacttcgc ggagagtctg cggattaaag
     7741 cccatctttt cgcgtatcac tccctcggcc agcatttcat cattaaattc acatttgtga
     7801 tataagattt tcagcaacgc ctgctggcgg ggaattttgg caatatattg caaaccgaca
     7861 atcaattttt cacgcaattg ctgaaacggg tcatgctcta acccagcggt caagtgatcc
     7921 tggattaact cccgcaatga aggctgttgc aaccacatct cattaaacag ttgagtcttg
     7981 ttttcgaagt gccagtagat agcgccacgc gtaacgttag cagcgtcggc aatgtcgttg
     8041 agcgtcgtct tgcttacgcc atactgcgca aactgggcga tggcagtttc aatcagttct
     8101 tgccgggtct tcagggcttc agctttggtt ctttttgcca tgattaatta ttcaggaaat
     8161 aaatatattc gacacaggat gagaaaatag cgaaggttaa tctatcacct aatgtgtatt
     8221 tatacgagag gctaatattg cgttgctata aatcgtcaaa taaatgatat gtattattta
     8281 cctaaaatac attcaccct
//
LOCUS       DAWDRC010000104         8296 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4189_length_8296_cov_3.297537, whole genome shotgun sequence.
ACCESSION   DAWDRC010000104 DAWDRC010000000
VERSION     DAWDRC010000104.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8296)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4189_length_8296_cov_3.297537"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         2261 a         2183 c         1809 g         2043 t
ORIGIN      
        1 aacggcgtac cgctaccgcc atgtaatgcc agaggcacgc tcgagcagcg cgaaatctcc
       61 gccagacgct gaaaatcaat agcaggtaca acgccaggcg cgtacatgcc gtgcgccgtg
      121 cctaccgaga cggccagtag atcaatgcct gtctgcgcga taaacggttc cacatcagtg
      181 ggttgcgtca tcaggtctgc attggcggcc tggtcgtaac gcggcgcatc tgccagatgg
      241 cccagttcgc cttccacgca gagctgcgcg ctggcggcga gttccaccac cgccctggtc
      301 tggcgcacgt tttcctccag gggaaaagca gaagcatcaa tcattaatcc gctgaaacca
      361 gcacgaaacg cctgacgaat acagtccgga tttttgccat gatcaagcgc cagagcgacc
      421 ggtactgagg cagattccgc gagcgtttta accactgccg ccgcgacggg tgcaggaaaa
      481 tgcgccagat gcccctgata gagattgaga atgatcggag cgcgattctt ctccgccacg
      541 cggatagctg cgtgagcact ttccagattc atacagttga tcgccagcaa ggcgtaatgt
      601 tcatgccagg ctttcatcac catacttttc atatcggcaa acatagggcc tccggttatt
      661 cgagggtgaa agtgatctct tcatctttca gcttttgatc gctcagttcg tcaaactctt
      721 cgtcgcgttc cgttaccggt ttcttccaga gtgaaagcat cacgccacaa atcaccgtgc
      781 ctatacccag cgccaggcaa aacagcagag gatgggtgaa cagaggcaca acaaacatgc
      841 cgccatgcgg aaccggactc tccacgcccc acaccatgat taagccgccc gcgaccgccg
      901 atgccaccac acaagatccc actactcgca gcaggtcacg cgcggcgatc ggaatgactc
      961 cttcagtgat catgcagatc cccatcggaa acgcggcttt cagcgcctcg cgctccgcac
     1021 gggtgtattt atggcgtgtc agtaagaacg aaagtgtgat gccaaacgga gggatgattg
     1081 agccgacaaa tttcacggct tctggtccgt agacgccact gaccagcaag ccatcggaaa
     1141 acagcgacat ggttttgttg accggaccac cgaaatcgaa ggttgccatc gcgccaagta
     1201 tggcccccat caggaagcgc gatccgccct gcatcgactc gagcaaatga atcagtgctt
     1261 cttgtaacca ggcgatgggt ttaccaatca gcgtcatcat taacagccca ccgataatgg
     1321 tgcttaatac cggcaacacc atgattggca tgagtccttg catcgactgc ggcaggcgga
     1381 tatagcgttt aagtagcaaa atggtgtagc ccaccaggaa accacccaac ataccgccaa
     1441 taaatccggt atggatttgc ccgcagagaa agccgacaat caggcctggg gcaaatccgg
     1501 gacgatcggc aatagaatag gcaattgccg cgctaatcag cggcacaatc agccccattc
     1561 cccagccgcc aatctgatta agcatccacg ggattgttcc tgttttttca ccaacatctg
     1621 taccgccaag aacttgcccc aacgcaatac atatcccggc ggccacaata aggggtatca
     1681 tccacgagat gccggttaat aaatgttttt taatttcctg taaaaccgga attttatttt
     1741 ctttcatatt aagatccctc cagtataagg atcatttgtt tcccagtgat ttttcgacct
     1801 tttcaagaaa ttgaacgggc gatttaatga cgatctctgt ctttactcgc acaatacgtt
     1861 tattggtgaa gcgttcttct ccttttattt tcacatctac agcaagaatc acgacgtcag
     1921 cagcagagat atctgccgcc tgtaattcat tttctgtacc aatggttccc tgggtttcaa
     1981 ctttaatggt atggcccaga gcttttgctc ctttgattaa cttttcacga gcaatatagg
     2041 tatgagcaat acccgccgta caggcggcaa cacagacaat attcatgaca tacttcctgt
     2101 tatttagcag acatcggatt gtgcatactg actaaagaga tcaatgattt tttgcggact
     2161 cgtctcaact aacaattgtt ctatgatgtc atcatccgcc agcgcgcccg cgacctggga
     2221 aagtaaacgg atatgggtgg tattttgatc ttcaagacgt accgcaaata aaataataca
     2281 gcgcacgcta ctgccatcca gcgtttccca gggaatatcc tggcgggtac ggccaattgc
     2341 cagcgtagta ttaattaccg cagaagattt accatgcggg atcgcaatat gattttcaaa
     2401 accagtcgag ccttccgctt cgcgctgcca gacatcttta ataaaggttt cccgacaatc
     2461 gatcgcacca tcttttttga gaagattagt taattcttca atcgcctccg ctttattcgt
     2521 tgcttgcata tctaaaataa cgcgattcgt attcaggatt tttgttatgt ccattcgtca
     2581 tacctcataa aattctgaca ttaaatttca acgacccgag acgtcgcatg atgtaatttt
     2641 tccgtaatca ccatttcagt gacccgctta atatcggcat cattaaaaaa agcggtaaca
     2701 taagcgaccg gcatagtgac tgtaggcaaa ttaatggtcg aaataatcag ttcgatattg
     2761 tcaggcaaaa cctgtgataa attcgctgct gaaataacat caacaatcgt ccattcagga
     2821 aatgctcgca gaatacggct tttcagtaga tgcgaagttc cgattccggt tgaacagacc
     2881 agtaatacac gtttacgggc aatttgccgc tccatcgcag cctgaaaatg aacggtcagg
     2941 taacccactt catcctcgct cactgcattc tcaccccagg ttttaaatac ctgattcacc
     3001 acatattgcg tcaaccgcca gacatccgct aattctgctt taatatcttc cagcaatgga
     3061 ttacggatat gaatgcgata atttagacgg ttaatcagcg gcttaatatg caccaataag
     3121 ccatcatata atgcgctgtc ttcactaaaa tcgcagtcca cgatgtcact gaataccgta
     3181 attaaacgcc aggtaatcag acgcgcttca ttgctggcct gcatatgtga aataatgcta
     3241 acatcttttt gtccatcaat cgccacacct gatgaaatga tatattgata aataaaccag
     3301 acttcatcat cgggcaatgt atgtgcaatt cgttgttcta tcttatgaat catctgactg
     3361 gcaacattaa agatattttc atcatattgc ctggtttgtt cttcttctct tgataacgca
     3421 ttcccgtgcg tgttgcgata catcataata aggatatgag taaaaatgtt aacgtaataa
     3481 ggttctccca aagaccaact taattcatat tccatatcca gtaacaatga ctggacaaat
     3541 aacacctctt cttctccaaa ataatggact aatgctttat agcttccggg atccagacgt
     3601 gaatacacga caccttgcgg ctcattatgg ttaataatgc cgttaatcag taatgccatt
     3661 gcctgtcgga cttgcccttc actaccttca atatgcgtac cacttggact gcggatcaac
     3721 gataacccca acggcgcaag ccaggactca attactctca ggtcattaac aatagaggct
     3781 ccgctgataa agtagcgttc tgacaattta ctgatcgacg tttcattcgg tgtttcactt
     3841 aataactgcg acgctatttt aacacgcctg gcattattca ttacaccatc aatctcttca
     3901 cattctgcca ccaacaaatg atcaagatgc agcaaatcag caatattctc cgcacttaac
     3961 atcacacccg cgccagcacg cttctcaaca tttattctcc attgtcccag ccattgctca
     4021 agtaaacgta aatcacgttg aatggttttt tccgaaacat ttaaataacc ggcaatcgta
     4081 acaatagtcg tatattccct tcgaggtaaa aggaatcgta ataatctgtt ttgtcgcgag
     4141 gtaatcattt gcatttttta cccttatcac agattaagaa gccccatatg gaagagcttc
     4201 agttgccatc tctgagggta aatataaagc agaataattt cacaacaata ctaaaatctg
     4261 tccgtaatcc ggacagtgat aaaacaaagc aaggcggtta agggatattt ttcagcacag
     4321 atcacatttt cgtgatgaat aatcgtgatc atgctcgcaa atacgatgga agaaatattt
     4381 attaccagat gtgagattat ttaccggata cgacacaaaa ccattcgtat ccggcattct
     4441 tcaatagatt acaacgtaat tgtcagcgca ttcccttgag gagtcaccac cagtccctgc
     4501 tcactttcag cctgcgaacc gccttgcaga ccatttactt tcacaatatt gcgcaagcac
     4561 agtgtccagt tcttcgcctc gcccgtacca gtcacagtaa tcgtgttgcc agtacgtgct
     4621 gcttttaaag taaagatcac cgaaccgtca gcatcgggca cttcacagac ggcttcatgc
     4681 ccgtcttgca gattaaagag gtggaatgcc gtaccttcgt gccacgcgta atcgggacgt
     4741 tgatcgttgt tgcccagcgc cagtagggtg ctatcacgca cataaacggg cagactcagg
     4801 aagctgtgct gctgtttatg ccagcgacta ccgtcgagtt catcgttgtg ccacaggtgt
     4861 gtccagcgac cttccggcag gtagaactgc acatcgcccg cttctgtgaa cacaggcgca
     4921 accatcacgt tgtcgcctaa catatattga cggtcaaggt aatcacaagc cggatcgtcc
     4981 gggaactcca tcatcatggc ccgcatcatc ggcgtacccc gggcgtttgc acgtgcagct
     5041 tcacgataca gatacggcat catgcggcat ttcagttgcg tgaagaagcg caccacatca
     5101 caggactcat catcgtaggc ccacggcaca cgataagatt tgctgccatg taaacggcta
     5161 tggctggaaa gcaaaccaaa cgcgcaccag cgtttgtaaa cgtgcgccgg agcggtattt
     5221 tcaaagccgc cgatatcgtg gctccagaag ccaaaacctg aaaggccaat agacaaacca
     5281 ccgcgcaggc tttccgccat tgattcgtag ttagcgtaac agtcgccacc ccagtgtacc
     5341 ggaaatttct gcgcaccaac ggaggccgag cgggcaaaca agacggcttc ttcctcacca
     5401 acggtgtcct tgaacacgtt ccacaccagt tcgttgtaga tgtacgcata atggttatgc
     5461 attttctgcg gatcggaacc atcaaaccac tgaacatcgg ttggaatacg ttcgccaaag
     5521 tcggtcttaa agcaatcaac gcccatcgcg accagacctt tcagtttgtc ggcgtaccat
     5581 ttgcaggcat ccggattggt aaagtcataa atcgccagac ctggctgcca tttatcccac
     5641 tgccacagcg aaccgtccgg gcgtttgagt aaataacctt tctcttgtaa ctctttaaag
     5701 acgggggatt tttgcccaat ataggggtta atccagacgc agattttcag ccctttcgct
     5761 ttcagacggc ggatcatccc ttccgggtcc ggaaaagtca gcgggtccca ctcaaaatcg
     5821 caccactgga aggctttcat ccagaaacag tcaaagtgga aaacatgcag cggcagattg
     5881 cgttccgcca taccatcgat aaagctgttt accgtcgctt cgtcgtagtt ggtggtaaat
     5941 gaagtggtta gccacagacc gaaggaccac gcgggcggca gcgccggacg gccagtaaaa
     6001 cgggtataac gatcaagtac cgctttcggc gtcgggccgt cgataacaaa gtattcgaga
     6061 tattcactct caacgctgaa ctgtactttg gagactttct ccgatcccac ttcaaaagag
     6121 acgcattgag gatgattgac cagtacccca taaccacggt tagtcatata aaacgggata
     6181 tttttatacg cctgttcagt acttgtgccg ccgtcccggt tccaggtctc taccgtctga
     6241 ccattgcgca ccagggcagt aaagcgctct cccagaccgt aaactgtttc gccaacgcca
     6301 agatccagcc gctcaaacat ataatttcgt tgattattcg tgtcctgcac gtagccatta
     6361 tttttcacct gactaccggt aatacgttca ccgctgcgca gaaaatccag tgaccagaac
     6421 tcacctttgc tgacacgcgc acttaagttg ccacttttaa actcagcgta acgttctgtg
     6481 ttttcgatag tgaccttcac gtcctgcaaa atattgagcg ggtaatgagg accgttattc
     6541 agcgccccct gaaaatgctc aatccgtaca ccgacaatac cttcctgtgg ggagaaaaag
     6601 cgcagcgtaa ataaaggcgt atcaagctgc caggtacgtt cacgcacatc acgaggggca
     6661 gcatagacca ccatttcatt accctgctgt tcaacctcga acacctgaag cgggtgaatc
     6721 aaattgaggc caggttgaat caaccagttt ccgtcgctaa ttttcattgc cgttccttag
     6781 ttctgcacat cttgagaggt gaattgttgc tggcaacggc gtttgccctg cgccagctgt
     6841 tccataacgg ttttcagatt gtgcgtcgtg agcgagtagt agcgtttagc gataatcgcg
     6901 ctcagcaagt aacagatcgc cggaacaatc gtgaatagcg cgataatgat gctaattgtg
     6961 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
     7021 agggcaccac cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
     7081 ccggtcaggc gtttaccatt acaccactcg ccgtagtcga cggtatcgga catcattacc
     7141 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacatg
     7201 gtgatgctgg cctgcatagg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
     7261 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagcggttt cgccagtgcg
     7321 gaaccaatca ggttacccac gcaataagtg gtgagaaaag caacaaacac ttccggcgta
     7381 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
     7441 ttgaaaattg tcaggaaacc gacaatacgc cactggtcgt tttgccagat atcacgtaaa
     7501 tcatcccgca tagacgtcgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
     7561 cagaatgcca gcatcataaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
     7621 agtggtttat tatcaccacc aattaaatta accagtggca tcatcagaac agtagagagc
     7681 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
     7741 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgatggt ataaagtaag
     7801 gtaagtagag tgtaagtaat tgctgcataa atcatttttc cgttcatact gagatctggc
     7861 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
     7921 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     7981 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     8041 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     8101 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     8161 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     8221 gcgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     8281 ggtagcgtta atttcg
//
LOCUS       DAWDRC010000105         8276 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4198_length_8276_cov_2.478896, whole genome shotgun sequence.
ACCESSION   DAWDRC010000105 DAWDRC010000000
VERSION     DAWDRC010000105.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4198_length_8276_cov_2.478896"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2396..2405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5375..5384
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2222 a         1691 c         1867 g         2476 t
ORIGIN      
        1 cttcaaatga cagatggtat gcatatcatc gttgaagcat taaaacagaa taattttgac
       61 actatttatg gtgttgtagg tattcctgtg acggatatgg cgcgccatgc ccaggcggaa
      121 ggcattcgtt atattggttt tcgtcatgag cagtcggcag gttatgccgc tgcagcaagc
      181 ggttttctta cccaaaaacc agggatctgc ctgacagtgt ctgcgccagg attcctcaac
      241 ggtttgaccg cattggccaa tgcaacggta aatggttttc cgatgatcat gattagcggc
      301 tccagcgacc gcgcgatcgt cgatctccag caaggtgatt atgaagagct ggaccaaatg
      361 aatgcggcaa aaccgtatgc caaagcagca tttcgcgtta atcagccgca ggatcttggc
      421 attgcattgg cacgcgctat ccgggtctca gtatcgggtc gtcctggcgg tgtttatctt
      481 gatttgccag caaatgtcct ggccgcgacg atggaaaaag acgaagcgtt aaccacgatt
      541 gttaaagttg aaatccgtcg ccagcattat tgccatgccc gaagtcagtc actagcgcaa
      601 tttcgctttt agcaaaagct gaacgaccat taattatcct tggcaaaggc gcggcgtatt
      661 cacaatctga tgaacagctt cgtgaattta ttgaaagtgc ccagattcca ttcctgccaa
      721 tgtctatggc gaaagggatc ctcgaagata cgcatccact ttctgcggca gctgcgcgtt
      781 cgtttgccct ggcaaatgct gacgttgtca tgcttgttgg tgcacgactg aattggttac
      841 tggcacacgg taaaaaagga tgggcggcag aaacacagtt tattcaactg gatattgaac
      901 cgcaggaaat tgacagcaac cgccccattg ctgtgccagt cgttggcgat attgcatcca
      961 gtatgcaagg tatgttggca gaattgaaac aaaacacatt tacgactcca ctggtgtggc
     1021 gcgatatttt aaatatccac aagcagcaaa atgcacaaaa aatgcatgaa aagttaagta
     1081 cagataccca accattaaat tactttaatg cattgagtgc cgtgcgcgat gttttgcgcg
     1141 agaaccagga tatttattta gttaatgaag gggcaaatac cctggataat gcacgaaata
     1201 ttattgatat gtataaacca cgtcgtcgtc tggattgtgg cacctggggt gtcatgggca
     1261 tcggtatggg ctatgccatc ggtgctagcg tgacctctgg ttctccggtt gtcgctattg
     1321 aaggtgatag tgcttttggt ttcagtggga tggagattga aacgatttgt cgatataacc
     1381 tgccggtgac gatcgttatt tttaataatg gcggcatcta cagaggagac ggtgttgatc
     1441 tcagcggcgc tggtgcacca tcgccaacgg atctgttgca ccatgcaagg tatgacaaat
     1501 taatggatgc gtttcgtggc cttggctata acgtcaccac gacagatgaa cttcgtcatg
     1561 ctttaaccac cggaattcag tcgcgcaaac cgaccattat taatgtggtc atcgacccag
     1621 cagcaggaac tgaaagtggc catattacca aacttaaccc aaaacaagtc gctggtaatt
     1681 aaatactgac atcgtctcct cttgccaact tattatgcga tggtgagggg acccttatcg
     1741 gaaatactat tatgctaaca ttttttattg gcgatttatt gcctattatc gtaatcatgc
     1801 tgttgggtta ttttagcggc agacgagaaa ctttttcaga agatcaagct cgggcattta
     1861 ataaactggt attaaactac gctcttcctg cggccctatt tgtttctatt acccgggcaa
     1921 acagggaaat gatttttgcg gacactcgcc tgacccttgt atcacttgtg gttattgtcg
     1981 gatgtttctt tttctcctgg tttggttgct acaaattttt taaacgtacc catgcagaag
     2041 cagcagtatg tgcattaatt gcaggctcac ctaccatcgg attcctgggg tttgcagttc
     2101 tcgaccctat ttatggtgat tccgtatcaa caggtttagt ggtagcaatt atttctatta
     2161 ttgttaacgc aattactatt cctattggtc tgtatttgct gaatccttcc tcaggagcgg
     2221 atggtaagaa gaatagtaat ctgagcgcat taatttctgc ggcaaaggag ccagtagtat
     2281 gggcacctgt tctggcaacg atcctggtgc tggttggggt aaaaattccg gcagcatggg
     2341 acccaacctt taatctgatt gcgaaggcta actcaggggt agcggtattc gctgcnnnnn
     2401 nnnnntggct gcacataaat tcgagttcag tgccgaaatt gcttataaca ctttcctgaa
     2461 gctgattctg atgccactgg cgctgcttct cgtaggtatg gcatgtcatt tgaatagcga
     2521 acatttgcag atgatggtac tggcaggcgc attaccgccg gcattctccg gaatcattat
     2581 tgccagccgg tttaatgtct acacccgcac tggtacagcg tcattggctg tgagcgtact
     2641 gggttttgtc gtcacggctc ctttgtggat ttatgtcagt cgactggttt cataattaat
     2701 catgttattg attttacata aatgccgaag tgtcttttca ccttcggctt ttttcaaggc
     2761 ataagcaaat gacaaataat gaatgcaaag ggccgtttga aggcttatta gttatcgata
     2821 tgacgcatgt ccttaatggg cctttcggaa ctcaacttct ttgtaatatg ggtgcaaggg
     2881 taattaaagt tgagccgccg ggtcatggtg atgatacccg cacatttggt ccctatgtgg
     2941 atggacagtc gctctattac agttttatta atcatggaaa agagagtgtg gttcttgatt
     3001 taaagaatga tcacgataaa agtatattta taaatatgct taaacaagct gatgtattag
     3061 ctgagaattt tcgcccaggt acaatggaaa aactcggatt ttcatgggaa aggttacaag
     3121 aaatcaaccc gcgccttata tatgcttcgt cgtcaggttt cggacatacc ggtccgctaa
     3181 aagatgctcc tgcctacgat accatcattc aggcaatgag cgggataatg atggaaacag
     3241 gataccctga tgctccgcca gtgcgcgttg gtacctctct tgcggatctc tgtggcggtg
     3301 tttatttatt cggtggaata gtgagtgcac tttatggccg cgaaaagagc cagagaggtg
     3361 cgcatgtcga tatagcgatg tttgatgcca ctctgagttt tctggagcat ggactgatgg
     3421 catatatcgc gactgggaag tcaccacaac gcctgggaaa tcgccatccc tacatggcac
     3481 cttttgatgt tttcgatact caggataagc cgattacgat ttgttgtggt aatgacaagc
     3541 ttttttctgc gttatgccag gcactggagc ttacggaact ggttaatgat ccccgattta
     3601 gcagcaatat tttacgcgta cataaccagg ctattcttaa agaatatatt gagcggacgt
     3661 taaaaacgca ggtagctgaa gtttggttag ccagaataca tgaagttggt gtgcccgtcg
     3721 cgccgttatt aagtgtggct gaggccatta atttaccaca aactcaggcg agaaatatgt
     3781 tgattgaagc cgggggaata atgatgccgg gtaatccgat aaaaatcagc ggctgcgcgg
     3841 acccgcatgt tatgccggga gcggcaacgc tcgaccagca tggggaacaa attcgccagg
     3901 agttctcatc ataaatgtaa gatgtgctgg taaatagctc ccacatttga acattgtggg
     3961 agccgctatt tagtcatttt gctgacagaa aacagtaatc tcctggtcca gctctgcaat
     4021 gtgttctttt acagagttca gcaactgaag aatttcaggc ttactgtcat ctgaaacagg
     4081 tgttatttct aactggtggc taatattaat caacttttgc aaattcagga tgttagccgc
     4141 accgtggatg cgatgaatac actgatggaa agttctgtta tcgccagctt ctagtgcatg
     4201 aaacgcagcg ggtagatctt tatgcgtttc atgctggaaa gtcatgagaa tctcctgcat
     4261 cagttgtaga tcattcgccg tattattctt cagggcctcg atatcaaggt ggcgatactg
     4321 aggtgcaata tacgcaacct ggtgtaactg acttaaatgt gttttcagta catccagagt
     4381 caacggtttg aacaaacata agttcatgcc gcaatttaac cctttttcac gttcgttagc
     4441 ctgtgcgttg gctgtaagcc cccagatggg taaggaagaa ttttgctcac ggagtttgcg
     4501 agtcaactca aaaccatcca tattcggcat attaacgtca gtaataagca gatcataatg
     4561 ttgcatactg actttgtgta gcgcttgcac accatcagtg gcttcatcaa catcatatcc
     4621 taatagattt agctggcgtt tgagtaatag cctattggtc ggatgatcat ccgcgattaa
     4681 tatgctcaac ttttcaggta gtgtgatagg ttgttctgct tttgcctcga cagccgccac
     4741 ttgttgggta atttctaccg ggattgtgat cgtaaatatc gttcctatgc ctggatgact
     4801 ttctaatgac aaatcgccct gcatgttttt aattaattct ttgcagatca ttaagcctaa
     4861 accagaacct gtttgctgac gacctgcact tgtttggcta tagcgtttaa acagttgttg
     4921 ttgttcttcc tgagataatc cacttccaga atccataatc gtcattttga taacagcgtg
     4981 gttgtcatca atgtgaccca gggaggtcgt aatttttact gccccctcgg tagtaaattt
     5041 gagagcatta ctcagtaaat tagataagac ctgcttaaac gcctgagggt cgatcttaac
     5101 caggtagcgt tcaggaagcg tactgctgca acttaatgcg atcgatttgc ttgcagcaat
     5161 cgcagcgaaa gagtgacaag tgttctggac taaagtaggg atatcgaccc attgtggttg
     5221 aagttgatag ttacccgatt caattttgtc gacatcaagg atttcaccaa ttaagccgag
     5281 gagtgattgt ccggtggcgt aggcaagtga aatcgcctcc acccgttgtt ccttgctaag
     5341 accagaaccc gacagaagtt ccaggaagcc catannnnnn nnnnagccca taatggagct
     5401 tattggtgtt cttatttcgt gactcattgt tgccagaaac tggctttttg cgacagttgc
     5461 attgatcgct ttatttctct ctacttcgag tgcatgaatt aaatcacgcg tctcggtaat
     5521 atcttgccaa ccacaaatat aaacagcatg ttcgcttgct ggcagattac ataaagtatg
     5581 ccagtgatta atgcatcttt tctcgatgcc attatcaatt tcaaatacct gtgtgtatat
     5641 tgttcggttt tctttcgttt ctgctgtgac ttcatgcgta ttagaaaaaa catctttaaa
     5701 gggggattca ctgttttcta atggcaacat tgcatttttg tagtaatcat cagtgaaata
     5761 atgttcaaat gcactattat gactaatgac attaccttgc cagtttacaa cataagttgg
     5821 attcggtaag gagtccgaga gtgctttccg gaatgatatt tggttttcta aatcaccctg
     5881 aataacttta cgacgacgaa ctgagcgtaa caggtagaat ccccataaaa ggctactgcc
     5941 aactaataaa acggataatg tcgtaacaat ataaaactgc tcgctataga ggtcccatgt
     6001 gtcaatggtc acattgggca ttttaatcca tttttcggtc aggcgcagaa cttcgcttgg
     6061 gggaattgca ttcagtgctt tattaataat atccttaagt tccggttctc cgcgaggaaa
     6121 agcgaacgaa agtgatgcat tctgaacgcc gggaataaga aaatgataaa gttcattagg
     6181 atagtaatgg tcgatcatgt aacgcgaatt taactgtgtc gcgaccagag catcaagttc
     6241 accttccttg accttgtgaa atgcagcgct ggcgttatcg acttttatcc actctacctc
     6301 cggatacatc tcttttaatt gcgaatgaag ctcataataa tatggaatgg caactttcat
     6361 tccttttttt aatgtttgtt cactgtcagg cgctttttgc atgacaaaaa cgtaaggcgt
     6421 tgttatgaag acttcagcaa atgaaacatt attttctcga tcttcactat aaatagcggc
     6481 gggcaatata tcccatccac cggggttaag ctgtgtccca gcatgaatat tatgtgaaac
     6541 ggtgatcgga gaaaaattta aacctgtttg caaggtaata atattaagaa tatcacccat
     6601 cacgccccga actgagccag tttcatctgt catagaatag ggtggcgagt aaggattttc
     6661 cagcaccttt aaatcgggat gctgcttaat ccactgtttt tcatgttcag tgagttctaa
     6721 tggtttgttc agaaaggcca ggtttcctgt atcaagccaa ttttgtgata cttcatagcg
     6781 aacttcattt gttaaagcat caacaaatcg attgagtact tcattaagaa cgattgaatc
     6841 ttttctggtt aataaaaagt tatattgacg cggtgagtta taatacttca ctacatttaa
     6901 ggagtgagta aaataacggg aaatcatact gctggtaatg atgttactac caataaagta
     6961 atcattctgt ccggctgaga cggatgctaa tgcctgatat aaatttgtaa aagagataat
     7021 tgttgctttt ggaaatgatt gatgaattac ctcgtctggg ggataatttg ctactcgagc
     7081 aatatttact ggttttgatg aggtaagcgg tcgcattgaa tcgtgaaggg tagtgaccag
     7141 cgccggaaag gtaattatca gaggattagt tgcagcaatg tcatcattaa gaggcggcga
     7201 agcaactaaa tgtgataaca ctatatcaac ttcaccgtct tccagcgcgt ccattgcttt
     7261 ttgatgatct gcgtattccc ggagtgttaa tttgatattt aacgctcttt ttaaaagatt
     7321 taaataatca gcattaatac cacgaatccg ttgctgcgaa tcggtatgca acaacgtagc
     7381 tgtttgggac ttgtgtactg caatcacaag attttttttg ctcgcgagcc agcgtaattc
     7441 cttgttgctc agacgtagtg gatcaagtgt gacacggttg ttactactga tgccacgata
     7501 ttcgatgtaa tctccgtctg cgaaacttat ggtcgaccaa agaccacaac agagaagaaa
     7561 aatatagggt aaaaacttca tgtggttagc cgattttgtt acgttgtgcg aatgtgtaaa
     7621 gatccatcag tgatttgcat tctaattttt ccatcaagcg acttttataa gtgctgacag
     7681 ttttgttgct gatgaacatt ttttcagcaa tgtcattatt atccttgcca tcaagaatat
     7741 accgcatgac actaatttct tgtttcgata aggagtcgag tttttgctgg tcggacgtta
     7801 aacttccaac aaaccggttg agagagaagg ggaaatagca gtagccattt tttgcagctt
     7861 caatagccgc aatgatattg ttcatgcctt cttttttact cacgaaacca ttagcgccag
     7921 catcagcaca atgtttcccg taaaaatggt catttttagc ggagacaata ataataattc
     7981 cgctatattg gcgctttctc agcgtttcta acacctggat accgttaact ccggggatat
     8041 cgacatcaat gatgacgata tcaggcttaa gtgtttccac ccgctgaacg gcgcttccgc
     8101 cttcagtcaa ctctgctaag atttcaatat cgtttttgat caataaatta cgaattgctg
     8161 cgatagcaag aggatggtca tcaataatta ttgcgttcat agattattcc ctttgcaatg
     8221 aagcatttcc cttctccctg tagtaataca gacataaggt atcatctcat tttctc
//
LOCUS       DAWDRC010000106         8227 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4232_length_8227_cov_1.946158, whole genome shotgun sequence.
ACCESSION   DAWDRC010000106 DAWDRC010000000
VERSION     DAWDRC010000106.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4232_length_8227_cov_1.946158"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    578..587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1090..1099
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3705..3714
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6903..6912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1917 a         1998 c         2254 g         2018 t
ORIGIN      
        1 taccgccggg gcgttagagg cattaaacct cagtttgcaa gcggtaactg aaccgggcga
       61 ttgggtgata gtagagaatc cttgtttcta cggcgcgttg caggcgctgg agcggttacg
      121 gctgaaggcg ttatcggtag cgacggatat taaagaaggg atcgatcttc aggcgctgga
      181 actggcgttg caggagtatc cggtgaaagc gtgctggctg atgactaata gccagaaccc
      241 acttggattt accttaacgc cgcagaaaaa agtgcggctg gtagcgttgc tcaatcagta
      301 caacgtaacg ctgattgaag atgacgttta tagcgaactt tattttggac gggaaaaacc
      361 gctgcctgcg aaagcgtggg atcgccacga tggtgttttg cattgctctt cgttttcgaa
      421 atgtctggtg cctggttttc gtattggttg ggtcgccgcc ggaaaacatg cacgtaaaat
      481 tcaacgcttg cagttgatga gtacgctttc caccagctca ccgatgcagc ttgcgctggt
      541 ggattacctt tctactcgcc gatacgatgc tcatcttnnn nnnnnnngcc tgcgtcgcca
      601 gcttgcggaa cgtaaacaac gcgcctggca ggctttgctg cgttatctgc ctgcggaagt
      661 caaaattcat cacaatgaca gcggttattt tctctggctg gagctccccg agccgttaga
      721 tgcaggagaa ttaagcctgg tggcactgac gcatcatatc agtattgcgc cgggtaatat
      781 gttttctacc ggtgaaaact ggtcacgttt ttttcgtttt aataccgcgt ggcagtgggg
      841 agaacgtgaa gaacaggcgg taaaacaatt aggcaaactt attcaagaac ggctgtaata
      901 gcgtttaatt taattcctct caggttgggt aatatgaatt tcgaatagca gtcatatatc
      961 ctaactcctt gtctatactc cagaagataa ccttacagac ggcataatgc gcggtagctc
     1021 acaacctgat taaattttct cagggccgaa ggtgtgcctg caagccgccg tctatggtta
     1081 aacaaggagn nnnnnnnnnc tggccgggat acatcgaacg cggacaaact gataaacagt
     1141 acgactgggt aacgcaattc gaaaaagaga caggctgcgc ggtgaatgtt aaaaccgccg
     1201 cgacttccga tgaaatggtc agtctgatgg ccaaaggggg ttacgatctg gttacggcat
     1261 ccggcgatgc ctcgctgcgt ttgattatgg gcaaacgcgt gcagccaatt aataccgcct
     1321 tgattcccaa ctggaaagcg ctcgatccgc gcgtggttaa aggcgactgg tttaacgttg
     1381 gtggcaaagt ttacggcaca ccttaccaat gggggccgaa cctgctgatg tacaacacta
     1441 aaaccttccc gacgccgccg gatagctggc aagtggtttt tgttgagcag aatctgccgg
     1501 acggcaagag caataaaggc cgggtacagg cttatgatgg ccctatctac attgcggatg
     1561 ccgcgttgtt cgtcaaagcc acgcagccac agttgggcat cagtgatccg tatcaactca
     1621 ccgaagaaca gtatcaggcg gtgctgaaag tgctgcgcga tcaacataat ttgatccatc
     1681 gctactggca tgacactacc gtgcaaatga gcgatttcaa aaacgagggt gtggttgctt
     1741 ccagtgcatg gccctatcag gccaacgccc tgaaagccga aggccagcct gtcgctaccg
     1801 ttttcccgaa ggagggcgtt accggttggg ctgacaccac catgctacat agcgaagcga
     1861 aacatccggt ttgcgcctac aaatggatga actggtcatt aaccccaaaa gtgcagggcg
     1921 atgtggcggc ctggtttggc tcgttaccag tagtgccgca agggtgtaaa gccagtccgt
     1981 tattaggcga gaaaggttgt gaaacaaacg gttttaacta tttcgataaa atcgccttct
     2041 ggaaaacgcc tatagcagaa gggggcaagt ttgttcccta cagtcgctgg acgcaggatt
     2101 acattgccat tatgggcggt cgctaacttc gctggggtgt tttatgacgt acgcagtgga
     2161 gtttgacaac gtctcacggt tgtacggtga cgtgcgcgca gtagatggcg tcagtattgc
     2221 gataaaagat ggtgagttct tctctatgct ggggccgtcc ggctccggca aaaccacttg
     2281 cctgcgcctg attgctggat tcgaacagct ttccggcggg gctatctcta tttttggtac
     2341 gcccgccagc aatctgccac cgtgggagcg ggacgtgaat actgtcttcc aggactacgc
     2401 gctatttccg catatgtcga ttcttgacaa cgtagcctac gggctgatgg tcaaaggcgt
     2461 gaataaaaag cagcggcatg caatggcgca agaggcgctt gagaaagtgg cgttggggtt
     2521 tgtacagcaa cgtaaaccgt cacaactttc tggtggtcag cgccagcgag tcgccatcgc
     2581 cagagcactg gtgaacgaac cgcgtgtgtt gttgctggat gaaccgctcg gcgcgctgga
     2641 tctcaaattg cgagagcaaa tgcagcttga gctgaaaaag ctgcaacagt ctctcggtat
     2701 cacttttatc ttcgttaccc acgatcaggg cgaagcgtta tcgatgtccg atcgggtggc
     2761 ggttttcaat aatggacgca ttgaacaggt cgattctccg cgcgatctct atatgcgccc
     2821 gcgcacgccg tttgttgccg ggttcgttgg tacatcgaat gtttttgatg gactgatggc
     2881 agagaaactt tgtggcatga cgggaagctt cgcactacga ccggaacata tccgcctcaa
     2941 cacccatggc gaaatgcagg ctaatggcac gatccaggcg gtgcaatatc agggcgcagc
     3001 gacccgtttt gaactgacac tgagcggcgg tgaaaaactg ctggtgagtc aggccaatat
     3061 gacaggcgaa gaactgcctg ccacgctcac gcccggacaa caggtgatgg tttcctggtc
     3121 gcgtgatgtg atggtggcgc tggttgagga gaggtgaatg gcgatgaacg tattgcaatc
     3181 accttcacgt ccaggtctgg gtaaggtgtc cggtttcttc tggcgtaatc cggggctggg
     3241 gctgtttttg ctgctgcttg gcccgctaat gtggtttggc attgtctatt tcggctcgct
     3301 gctgacgctg ttatggcagg gcttttatac ctttgacgat ttcaccatgt cggtaacgcc
     3361 ggaactgacg ctggcgaata tccgtgcgct gtttaatccg gcgaattacg acatcattct
     3421 ccgcacgctg accatggctg tggcagtcac tatcgccagc gccattctgg cttttccaat
     3481 ggcgtggctc agtggttttt acaacatttt gggctggaac cactgctgac tgcgttcctc
     3541 acgctaccgg cggtgggagg taatacgctg tcaacttccg ggctggggcg ctttctggtg
     3601 tttctctata tctggctgcc gttcatgatc ctgcccgtgc aggcggcgct tgagcgtttg
     3661 ccgccgtcat tgttgcaggc gtcggctgat cttggcgcac gtccnnnnnn nnnnaacctt
     3721 tcgctatgtg gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt
     3781 ctcactcacg ctgggcgatt ttattgtccc gcagctggtt ggccctccag gatattttat
     3841 cggcaatatg gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt
     3901 caccctggtg ccgattgttc tcatcgcact ttacctggcg ttcgtgaaac gtctgggagc
     3961 gttcgatgca ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgtgg
     4021 ttttcctaca ttttcccatc ctgattatcg ctgcctatgc gtttaacact gaagatgcgg
     4081 cgtttagttt tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta
     4141 gtgatattct tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaatcg
     4201 cgctggtgtt agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa
     4261 acgccatttc gctgttactg ttgctgccca ttgcgctgcc gggcattgtc actggtctgg
     4321 cgctattaac cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg
     4381 gtcatgcgac tttttgcgta gtggtggtgt ttaacaatgt catcgcccgt tttcgccgca
     4441 cctcgtggag tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc
     4501 gctacgtagt gttgccgaat ctcagttcgg cgttactggc tggaggaatg ctggcgtttg
     4561 ccttgtcgtt cgatgaaatc atcgtgacga cttttacggc aggtcatgaa cgaacgttac
     4621 cgttgtggtt gctcaatcag cttgggcgac cgcgtgatgt accggtaacc aacgtggtgg
     4681 cactgctggt tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg
     4741 aaggcgacaa tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga
     4801 ttaacggaga actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg
     4861 gggacgtttt actggagatt gccgaggcat ccgcagagca ggtcaatgct gctgttcgcg
     4921 cggcagatgc agcatttgcc gaatgggggc aaaccacgcc aaaagcgcgt gcggaatgcc
     4981 tgctgaaact ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc
     5041 gtaattgtgg caaaccgctg catagtgcgt tcaatgatga aattccggcg attgtcgatg
     5101 tctttcgctt tttcgccggt gcggcgcgct gcctgaatgg tctggcggca ggtgaatatc
     5161 ttgaaggtca tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac
     5221 cgtggaatta tccgctgatg atggctgcgt ggaaactggc tccggcgctg gcggcaggga
     5281 actgcgtagt gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc
     5341 tggcgaaaga tatcttcccg gctggcgtga ttaacgtgct gtttggcaga ggcaaaacgg
     5401 tgggagatcc gctgaccgga catcccaaag tgcggatggt gtcgctgacg ggttctatcg
     5461 ccactgggga acacatcatc agccataccg cgccgtccat taagcgtact catatggaac
     5521 ttggtggcaa agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag
     5581 gtgtacgtac ttttggctat tacaatgccg gacaggattg tactgcggct tgtcggatct
     5641 acgcgcaaaa aggcatttac gatacgctgg tggaaaaact cggtgctgcg gtggcaacgt
     5701 taaaatcggg ttcgccggat gacgagtcta cggagcttgg acctttaagc tcgctggcgc
     5761 atctcgaacg cgtcagcaag gcggtagaag aggcgaaagc gacagggcac atcaaagtga
     5821 tcactggcgg tgaaaagcgc aagggtaatg gctattacta tgcgccgacg ctgctggctg
     5881 gcgcattaca ggacgatgcc atcgtgcaaa aagaggtatt tggtccggta gtgagtgtta
     5941 cgctcttcga caacgaagaa caggtggtga actgggcgaa tgacagtcag tacggacttg
     6001 catcttcggt atggacgaag gatgtgggca gggcgcatcg cgtcagcgcg cggctgcaat
     6061 atggttgtac ctgggtcaat acccatttca tgctggtaag tgaaatgccg cacggtgggc
     6121 agaaactttc tggttacggc aaggatatgt cactttatgg gctggaggat tacactgtcg
     6181 tccgccacgt catggttaaa cattaaggat aatattgcag atcgtaagag tatgagatga
     6241 tcttgcgtac tgtcagagca ctgatttctc tgacagtacg tgctgcgtgc aaaatcacta
     6301 catcaaataa accagccaaa tctcatagag ataagagtaa acaaaccaac agcagcaaag
     6361 acatttatca gtactacagt tctactggaa acattcattt ttgccacctt ttatcattgc
     6421 ccctatgtgt atagtacggg ttaagaaaat ccgcaataat actcattctg aagatagata
     6481 agggcaagtt gctgtttgat gattttttct ctaactggtg aaaaaacttg atgcacgtca
     6541 aaaaatgacg catatttgcg cgcgttttat tcatctggct ggacgcccgt acatgtctct
     6601 ctatcaacat atgcttgttt tttatgcggt tatggcagca atcgcatttc ttatcacctg
     6661 gtttctttct catgataaga aacgcatccg tctcttaagc gcctttctgg tgggggcaac
     6721 atggccgatg agtttcccgg tggcgctgtt gttttctctg ttttaaatct tatgcactgg
     6781 cttgacggca ccacgcagac ttatatcatt tggatgaatc gataaatttc acaagtggct
     6841 aaggagaaag tatgtcgcat ctggatgaag tcatcgcgcg cgtggatgcc gcgattgaag
     6901 agnnnnnnnn nngcgtcatt gcccatatga acgaattatt aatcgccctg agcgatgacg
     6961 cggagttaag tcgggaagat cgctacaccc agcagcagcg tttgcgcacc gctattgctc
     7021 atcacggtcg caaacataaa gaagatatgg aagcgcgcca cgaacagtta accaaaggcg
     7081 gtaccatcct ctgattaaaa tgagcgtctg gccaccagcc aggcgccaac cacaagtaaa
     7141 attgcgccac aaattgggcc gatcaacgcc cgaagtggta cgccatggct gcggaagata
     7201 tccagcacca gaccgccaat cagctggctg gcgaccagta ctgcaatagt tgtcgccgct
     7261 cccacattct gatatccgct gatactggcg aagacaaaaa acgatcccag caagccaggt
     7321 ataagtgtcc accagcgcac gctggacacc agttcgccaa accccgccat gccttgttta
     7381 aaccataaaa tagaaacaaa gagcacaatc cccaccagtg agttcagcaa catggcgatg
     7441 agaatggtag aggaggtctg ggtgatgcgc accatcagcg tgttctggac taccagacca
     7501 atcccggctg caatcaaaaa ggcaagggta agtgactgat tcatccgatc gcgtccggtt
     7561 cagtgcgctc gtcgagttgc aactgcataa atgtcagatc cagccagcgc ccaaatttag
     7621 tacctacctg cggcatttgc gcggtgacga caaatcccag cgactggtgc agatgcagcg
     7681 aggcctgatt ttgcgattcg atcccggcga ccatgacatg cttcccgcaa tcccgcgctt
     7741 catcaatcaa tcggcttaac aatttacgcc ccagaccttt cccctgatga tcgggatgga
     7801 cataaaccga atgttccacg gtatggcgaa aaccatcaaa actacgccag tcgccaaacg
     7861 aggcatatcc cgtcactacg ccatcttctt cactcaccag caccggataa cctgctatgg
     7921 tccgcgcttc aaaccaggca atgcggttat ccgcatccac cgtttggtca ttccagatag
     7981 ccgccgtata caacacggcg tggttataaa tttccgcaat tgcagcacag tcggctttgc
     8041 gggcaaaacg gatggacatg tctggcctcg ataaaataat gatgatgatg ttatcaatac
     8101 tatcgaaaac agcccgaagc aacggattcc tctatgaaat tattgcgtgt tgcagagggg
     8161 atgtgacggc tgcaaacaaa accaatcctg tgagcctgct actattgatg gattcatgcc
     8221 aaaagca
//
LOCUS       DAWDRC010000107         8222 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4236_length_8222_cov_2.407861, whole genome shotgun sequence.
ACCESSION   DAWDRC010000107 DAWDRC010000000
VERSION     DAWDRC010000107.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4236_length_8222_cov_2.407861"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    182..191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2754..2763
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6504..6513
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2155 a         1968 c         2016 g         2053 t
ORIGIN      
        1 acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccgcgtt
       61 gtgttccgtt gggaacgagg cgaatattac ggattgcctc acccttcgtc aacggttttt
      121 ctcatctttt gaatcgtttg ctgcaaaaat cgcccaagtc gctattttta gcgcctttta
      181 cnnnnnnnnn ncgtgcttcc tgtgctgcaa gctcttcatc tgtcgcaaaa acaacgcgta
      241 acttacttgc ctgacgcaca atgcgctgaa ttgttgtttc accttgttgc tgttgtgtct
      301 ctccttccat cgcaaaagcc atcgacgttt gcccaccagt catcgccaga taaacttccg
      361 caaggatctg ggcatcgagt aatgccccgt gcagcgttcg tttactgtta tctatttcgt
      421 agcgggcaca taacgcatcg aggctgttgc gcttaccggg aaacattttc ctcgccaccg
      481 caaggctatc ggtgacctta cagaaggtat tggtcttcgg aatatcgcgc ttaagcaacg
      541 aaaactcgta gtccataaag ccgatatcga acgctgcgtt atggatcacc agctccgcgc
      601 cgcggatata gtccatgaac tcatcggcta cctcggcaaa cgtgggctta tcgagcaaaa
      661 attcatcggc aataccatgt acgccaaagg cttccggatc caccagccga tcgggtttga
      721 gataaacatg gaagttattg cccgtcaggc gacggttcac cacttcaacg gcaccaatct
      781 caatgatctt gtggccttca tagtgcgcac caatctggtt cataccggtg gtttcggtat
      841 cgagaacgat ctggcgtgta attgcagtgc tcatagcggt catttatgtc agacttatcg
      901 ttttacagtt cgattcaatt acaggaagtc taccagagat gcttaaacag gtagaaattt
      961 tcaccgatgg ttcgtgtctg ggtaatcctg gacctggggg ttacggcgct attttacgct
     1021 atcgcggacg cgagaaaacc tttagcgctg gctacacccg caccaccaac aaccgtatgg
     1081 agttgatggc cgctattgtc gcgctggagg cgttaaaaga acattgcgaa gtcattttga
     1141 gtaccgacag ccagtatgtc cgccagggta tcacccagtg gatccataac tggaaaaaac
     1201 gtggttggaa aacagcagac aaaaaaccag taaaaaatgt cgatctctgg caacgtcttg
     1261 atgctgctct ggggcagcat caaatcaaat gggaatgggt taaaggccat gccggacacc
     1321 cggaaaacga acgctgtgat gaactggctc gtgctgcggc aatgaatccc acactggaag
     1381 atacaggcta tcaagtcgag gtttaagcct gtggtttacg acattgccgg gtggcaccaa
     1441 ccgcctggcg aattcgcggt ttgttcttgc tctgtttcat cggatttagc gttaaaggaa
     1501 tagtccgttt ccgggcaaca ataagttgta agcaaccaag cgcaggaata tgcgcattca
     1561 atagttttcc tccgtgtttg ttccacggga gaacgtggaa acggctggcg tgtagcactt
     1621 caaaattcaa caaagagagc cagtccagct gccgcatcag agtgaacatc cggctgttat
     1681 agggcgatgt tttgcgcaat accggcacaa gtttgcgtaa tcccattaaa ctgatgggat
     1741 tgaagccact aatcaccagc cagccatcat caatcagtac ccgatcggct tcacgcaata
     1801 aacgatgcgg atcagtgcac cacggcaatg tatgtgccag tagacaaaca tcaacggatt
     1861 tatcggcaaa aggaagatga agtgggtccg cctgcacctg gacgggcatt ccttgcgcag
     1921 aaacattcac ttgatgagaa accgcgcacg cttcgcaatt gatttctgcg cttaaattgc
     1981 caatcttaag cagatgaaaa ccatacattt tagtgaacca cgggttgagc tgacgctcca
     2041 gcgccttgcg ataaagctct ccccagggca aatcgcccca gcaatcagga gccacgacag
     2101 tttgagggac tcttgccggt ttcatcacaa ccttccgttt cacactgaga ggtaatctat
     2161 gaatcttaac agtattcccg cctttgatga caattacatc tgggttttga atgatgaagc
     2221 aggtcgctgt ctgattgtcg atcccgggga cgcagagcca gtattaaacg ccatttccgc
     2281 caataactgg caaccggagg ccatatttct cacccaccat caccacgatc acgttggagg
     2341 cgtaaaagaa ctggtggaaa agtttccgca aattgtggtg tatggaccac aagagacaca
     2401 agataaggga acaacacagg tagtcaaaga tggcgaaact gccttcgttt tggggcatga
     2461 atttagtgta attgccacac cgggtcacac tttaggacat atctgttact tcagtaaacc
     2521 ttatctattt tgcggcgaca cgctgttttc tggtgggtgt ggtcggttgt ttgaagggac
     2581 accatcacaa atgtatcaat cacttaaaaa gttaagtgcg ttacctgacg atacattggt
     2641 atgttgtgct catgaatata ccttatcaaa tatgaagttt gctttgagta ttcttccgca
     2701 cgatttgtcc ataaatgatt attatcgtaa agttaaggag ttacgggcaa aaannnnnnn
     2761 nnnatcaaat aacactaccc gtaattctga aaaatgagcg gcaaattaat gtttttttaa
     2821 gaacggaaga tattgattta attaatgtaa ttaatgaaga aacattattg caacaacctg
     2881 aagaacgttt tgcatggtta aggtcaaaga aagataggtt ctgataaaac tttcttgtca
     2941 tcggctccgt tcgccgttat gatcggtcgt cttttaagca actattgaca cacacatgaa
     3001 ggcaaaagcg atattactcg cctctgtcct gctcgtgggt tgccagagca ccggcaacgt
     3061 tcaacagcac gcacagagcc tttctgcagc tggtcaaggg gaagcagcaa agtttacaag
     3121 tcaggcacga tggatggacg atgggacgtc tatcgcgcca gatggtgact tgtgggcttt
     3181 cattggcgac gagctaaaga tgggaattcc ggaaaacgac cggattcgcg aacagaaaca
     3241 gaaatattta cgcaataaga gctatctcca cgatgtaact ttacgggcag agccgtatat
     3301 gtactggata gccgggcaag ttaaaaaacg taacatgcct atggaactgg tactactacc
     3361 catagtggag agcgcttttg atcctcacgc aacgtctggc gccaatgccg cgggcatctg
     3421 gcagatcatt ccgagcacgg ggcgcaatta tggtttgaaa cagacacgca attatgacgc
     3481 gcgtcgcgat gttgttgctt caacaactgc cgcgctgaac atgatgcagc gtctgaacaa
     3541 gatgtttgac ggcgactggc ttctgaccgt agcggcttat aacagcggcg aaggtcgggt
     3601 catgaaggca attaaaacga acaaagcgcg tgggaaatcc acggacttct ggtcgttacc
     3661 gttgccgcag gaaacgaagc agtacgtgcc taaaatgctg gcattgagtg atattctcaa
     3721 aaacagcaag cgttatggcg tacgtctgcc aacgaccgat gaaagccgtg ctctggcgcg
     3781 tgtgcacctg agcagcccgg ttgaaatggc gaaggttgca gatatggcgg ggatttccgt
     3841 cagcaagctg aagacattca acgctggcgt gaaaggctcc acgctgggcg caagtggtcc
     3901 gcagtacgtg atggtgccaa agaagcatgc agatcaactg cgtgaatctc tggcttcggg
     3961 cgaaattgct gctgtacagt cgacgctggt tgccgacaat acgccgctta acagccgtgt
     4021 ttacaccgta cgctctggcg acacgctttc aagtatcgct tcacgtctcg gcgttagcac
     4081 caaagatttg cagcagtgga acaaactgcg cggatctaag ctgaagccag gccaaagctt
     4141 gacgattggc gcaggtagta gcgcacagcg gttggcaaac aacagcgata gcattacgta
     4201 tcgtgtgcgc aaaggcgatt cgctttcaag cattgctaaa cgccacggcg tgaacatcaa
     4261 agatgtgatg cgctggaaca gcgatactgc gaatctgcaa ccaggcgata agctgacgtt
     4321 gtttgtgaaa aacaacaaca tgccagattc ctgacaaacc agataatgaa aggcaccgat
     4381 tcccccggtg ccttttttat ttatgctgct ttatgtgctt ctaccatgat gatatcactg
     4441 gtgaaagagc catcattctg caaggcaaaa tacgttttca cctctgtcga tgcgctctgt
     4501 tggtaaatgc gaatagcgtc tactaacgct tctggcgtac gcattctcgc gacccatgaa
     4561 gaaaattcca gcggtaactt atctgtaatt aaattatcaa ctatcagatt ggcttcattg
     4621 attaacctca accactcacc gctggcgtag tttcgtacgt gagaggtatc gcgtaatgct
     4681 tctaccgtct gtaaccagat gtcgcgcact gggtggcccg gagacattac gtccatcaca
     4741 atcagcctac cgccaggttt caatatccgg ttcacttcac gcagtgctgc accaacatca
     4801 tgccagtgat gggcagaata acggctgata acaatatcaa atgcgttatc ggcaaatggc
     4861 agactttcgg cataaccctg gcgggtggtg atatttttca gttgccgggc ttcggcagct
     4921 tgtgccacga catccagcat atgggcagat aagtcatacg ctaccaccgc gctcacgttt
     4981 tgcgcagcaa caaagctggc atgccctgct ccgcagccca tatcaagtac acttgcatca
     5041 ggataatcag ccagacgcac cgccaggcgc tgtaaatctc gtccggatgc atgtacggta
     5101 ctggtcagat attcgctggc ttgagagcta aactgctttt ctacgtggtc atggtgggat
     5161 tgtgttgtca ttgcgctgtc cttttgttgt ttgagtataa aagggcagcg ccaaacgttg
     5221 gtctgccctg tggcagacct gacatacctt atttatcagg cttgccggga ctgaattcaa
     5281 cgagtagcgg attgtgatcg gaagcgcgcg taaccagtac agaagcttcg ctgacgttca
     5341 gaccacggta gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg
     5401 taaaacgcac ctggcgtagc gacatttccc gcgcaaagcg atataacgcg ttcatccttc
     5461 tacggctcca ggcattgaaa tctcccgcca taatgaccgg gccactgtgg tgagctatct
     5521 gatcgccaat aggaagtaac tgcttactat agacatccac gcccagactg aagttgacgg
     5581 cgtgtatatt aaccaccatc aacaggcggg tgtcaggtaa gggatagacc gtcaccagtg
     5641 ccgatttcgc cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa tacactggat
     5701 gtgccgccga aagggtcatt acgccagaag gatgttgtgg cagcacgaaa gcgggtacct
     5761 gatcggcggc aagatagtta gcagtcgcaa actgtactaa ctctggcgtt gtctgcgctt
     5821 cctgcaataa caccagatgt gcatctttgc cgtagttctt taataccgac aaccattcag
     5881 cgcgttgctg tttgtatatg ttccacacca ggatccgaat acgctcttcg gtacttaacg
     5941 gttctccagg tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt aagatccttt
     6001 ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt ttttcgcact tttgactttg
     6061 aaacctctat cacgtcaacc cttccgcact cgggagggtt ctactgttat agggatttta
     6121 gctcactctt tcaacgagca attgtatatt gttatgtaag caagtgctta caccggtcag
     6181 gattttgctc attattgaga acattaaatc ggatggcata tctcgcatcc tgatggttta
     6241 atgacaaata aattcaaaat atctcttcta ttttgcaaca ggagcaaaaa tgaaagccac
     6301 gtcggaagaa ctcgccattt ttgtttcagt cgtcgaaagc ggcagcttta gccgggcagc
     6361 ggaacaatta gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc tggagatgaa
     6421 acttggcgtt agcctgctta atcggaccac gcggcaactt agcctgacgg aagaaggcga
     6481 gcgttatttt cgtcgcgtac cgtnnnnnnn nnncaggaga tggcagcggc agaatcagaa
     6541 attatggaga cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc aactccagtg
     6601 gtactgcact ttctgatgcc gttgattaag ccttttcgtg aacgctatcc ggaagtcact
     6661 ttatcgctag tctcctccga aacgattatt aatttgatcg aaagaaaagt ggatgtcgcg
     6721 atacgcgccg gtacgttaac ggattccagc ttacgtgcca ggccgttatt taacagttat
     6781 cgaaaaatta tcgcctcccc cgattatatt tcctgctacg ggaagccaga aacgatcgac
     6841 gatttaaagc aacatatttg cctgggattc actgaacccg cttccctcaa tacctggccg
     6901 atagcctgta gcgatggaca attacatgag gtgaagtacg gtttgtcatc caatagtggg
     6961 gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc cgactacatg
     7021 atcgacaaag aaatcgctcg cggagaattg gtggagttaa tggcagataa agtgttgcca
     7081 gtagaaatgc cattcagtgc agtctattac agcgaccgtg cggtaagtac gcgcatccgg
     7141 gcttttatcg atttccttag cgggcatgta aaaacagctc ccggaggagc tgtcagagag
     7201 gcttaatccc attcaggagc cagaccttcc gggctaacca ggcggtcgtt gcaatccagt
     7261 gcggcgatcg cttttttatc ttcggcatca agctgtaaat tttgtgcctt aagattactc
     7321 tccaggtttt cacgtttagt agaagaagga attactgagt aaccttcccc catagcccac
     7381 gccagaatca cttgagccgg agtcgcattg tgtttagctg caatacgagc aataacctta
     7441 tctttcagtg cattgccata cgccagagtc atataggaag taatatggat accgtgctgt
     7501 ttagcccaag cgaccacttt acggttttgc agataaggag agagttcaat ctggttagta
     7561 gcgatgtttt cagcgccaac agcagcaatc gctttttcca tcaatgggat cgtgaagttg
     7621 gaaataccga tctcacgcgt cagcccttgt tttttggctt ccagcagcgc ctgcataaac
     7681 tcttcagcag agacttcatc gcttggtgac ggccagtgga ttagcgtcag atcaacataa
     7741 tcggtacgca atttttgcag gctctctttc agactcggga tcaatttgtc tttgctgaga
     7801 ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc actttctgca
     7861 atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat tgcgcgataa
     7921 tcaagttcaa gcgccgtttt cacagatgaa ataacaacgt cgtctttcag acggaaagtg
     7981 cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt tcgttaactg
     8041 aggtgattat gggcttcaga ctgttgtgga aaaatggcgt aaaacgcaga ggatttttgc
     8101 gattttggca ataatagaaa tacaaacgaa aaaaaacctc gagcgttaac tcgaggcttc
     8161 ttaataagtg gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta
     8221 tt
//
LOCUS       DAWDRC010000108         8209 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4246_length_8209_cov_2.703213, whole genome shotgun sequence.
ACCESSION   DAWDRC010000108 DAWDRC010000000
VERSION     DAWDRC010000108.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4246_length_8209_cov_2.703213"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2251..2260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3022..3031
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2127 a         1999 c         2072 g         1991 t
ORIGIN      
        1 tcagacggta caggaacgct tacagcaaaa tggcgtaaaa gtcttttaac agggaataaa
       61 gttatgagag aaaaacccag tttttatgtc gcgcttacac cgatcatttt tatgatgatc
      121 gtgttggttg ttggcatcgg tgttatgggc tggcctgcca gcgtctgttt attaatttca
      181 gcggcgtttt cctgcattat tgcaatggct aagctgaaat atacctggga tgaaatccag
      241 gggtttatta tcgataagat ttcggcggtg atggcaccag tacttatcgt tattttcgtt
      301 ggctttatgg tcgcgacatg gagctatgca ggtacactgc cgatgctggt ttattacggt
      361 atgttactgg ttgcgcccgc atggctttat gccatagcgt tttttctgaa cgcagtattg
      421 tcctatgttt ccggggcatc atggggatct gtcgcttcta ttggtgttgc attaatgggc
      481 atcggttccg gcttacatgc tgatcttcca attctggcgg cagccattgt taccggggct
      541 tattttggcg acaaactttc accgttaagc gataccacta acctgacttc agcagtaact
      601 aaaactaaac tttatgatct gattaagtat ctactctgga cgaccatccc atcaacgatt
      661 attagcctgc tgttttttac ctggctggga atgattcata cttctacggg ctctatttca
      721 ccagaaagca tgcagatgct ggtacagctc aaagagttat ataagtttgg tctgttaccg
      781 ctactgccta tgcttattgt tctgtgcttt acctttttgc gtttgcctac agtaccggca
      841 ttattaagca gtgcgttcgt ggccgtattg gttggatggc tgtatcaagg tttcccgctg
      901 gccgaaggta ttaaggccac aatgagtggt ttccgtctga cgatgatcac tgattcaacg
      961 acgctgttac cttctgtgaa agaactgctt gaacaaggcg gcctgaataa tcagggcgga
     1021 ttcctgtcat ttattatctg cgccatgatg tttgctggca tcctgacggg aacaggaatg
     1081 atggatgtca cattacaaag tgcggcaaac aaaataaaaa gtgcctttgg cgctattctg
     1141 gcaagcggtg ttttagcgat catcattaac ttacttaccg gttctgatgg tttgaataag
     1201 attgttatat ctgaattgat gatgaagaaa ttcgaagatc ttaatttatc accgttagtc
     1261 ttagcccgaa cgctggaaga ttttggcacc atgagcgcgc caattattcc gtggtcagct
     1321 gccggattgt atatggcgac aacactaggc gtgccgacgt ttagttactt gccgtactgt
     1381 gtattctgtt tctgctcgat gatatttgcg ttgatctatg cttctacagg tttccgttta
     1441 ctgcgcttca acgaagcaaa ggcctgagtt attcaagata ataaatgcgt ttgccggatg
     1501 gattaatctc catccggcgt taatgtcaaa attccatgtt ttgcagaata atgtcagcta
     1561 agcacaatct ccagctcgcg cagttcacgc cagaagcgtt cggcaactgg attcatgcgg
     1621 gtattcatcc ggtatgcata ggcctgaatc gggataacta attcgtcccg attcagtaca
     1681 acgagctgcc cgctgcgaat ttcttgttgg atggcatact ccggcaacca ggcaatccca
     1741 catccgtcga gggcaacctg ctttaaaagc tcgctcatcg aagagacaaa aaaggtgctg
     1801 aaactcaact cactgtggcg cgtcagagtg cgattaatca atcgccccat gtaggagttg
     1861 cggctgtaat tcagtaacgg aaagtgtggc tgagcgaggt taaaaagtgc ttctccgtgt
     1921 tcgtcactgg cgcagacggg gaacaattga gattcaaata agcggatgtg gtcaaacggc
     1981 gcttccagca aatcttcgtc gtgaaaggaa aaaatacagt cactttgccc ttcgcgcagt
     2041 ttatcgaccg cttcatcgac atcaatggct tcaattgccc aggtaaagag cggcggcatc
     2101 tggctgataa tggacggtaa cagaccgagg gaaagagagt gtgcagcggc tatcttgatt
     2161 ttacgttgcg cgtaatcgct gccgccacgc agctctgcca gattgctctc taactgttgc
     2221 aacagatggc ggatctgcga atggaagatt nnnnnnnnnn tgttctgaga gttgcagcgg
     2281 cgttacctgg cggttaaaca attcaacacc aatcgcctgt tccagcgcac ggatgcggcg
     2341 gctgaatgct ggttgcgaga cgttgcgact gactgccgcc tgggaaaaat tgcgacattt
     2401 ttccagggtc agaaaatcat aaagccattt ggtttcaatg ttatgcaaaa ccgcaccaca
     2461 gtcatccatt acgttatact ctgtcagccg cccttgcggg cattctacgt ccattcgggc
     2521 ggctgacaac cgtttatgcc gtttcaaacg ttccttttac gcaggctttg ccgtctttga
     2581 ccatcagttt gccgcgagcg tatacctgct caatgcgcaa ttcaggcgtc atgaccagta
     2641 agtctgcgtc attgcctggc agaatttcgc ctttcccggt caggttaagg aaaccggcta
     2701 cgctactggt gagcgggcgc agggcatcgc taaggctgaa gctatagtct ttgaccaaca
     2761 cttgcacggt ttccagcaat gtttcaaaac ctgcaacacc gatatgggtt aaatttcctt
     2821 cgtcatcaaa gaacggctgg ctaccgttgc cgtcggagct gagggtgacg cgtgccagcg
     2881 gaatacccgc ctgaacggcg cgggcaatac cttcggcagg ggcgaccggt tcgtcaatgc
     2941 tgctggtgat gtcgatggtg ccgcctttgc gcgcgaactc cagcgcctgc tcaaacaacg
     3001 gaacgttgcg gttaacgtgg gnnnnnnnnn ncagagatgg cgcatttcac accaatcacg
     3061 cgatcgataa tcgccacgtc tttttccacg gaacccgtaa tagtgcggga agggacatga
     3121 taagcgccgg tcagcatcca ggcgctgatg ccttcttcat tgagcgcacg ggtcttggcg
     3181 agcagggatt ccgggtggcg agagatagag tcggtgccca gcaggccaac cactgacgtg
     3241 atgcccgctt ccgtcagacg acttaacgcc acttccggcg tgcgtgtcgt gggacctgct
     3301 tcgccgccac caccaatcaa atggacgtgt tgatcaataa aacctgggca gaggatctgc
     3361 ccgctgagat cgacaaccgt gcagtccggt acaatgtcag aagggatgtt gctggcaacg
     3421 gcaataattt tgccgttagc gatgaggaca tcgcaaattc cccgatcttc cggcgcatac
     3481 aaatgcgctc cctgcagcag ggtaaaaccg gctgcggaat aatcaatcat ggtaactcct
     3541 tgttaaacaa taagctgcat aacccagatc gacagcaatg cgttaatcac gcagaccgta
     3601 atgatatgcg ggtaatattt ggcattcact tcggctgttc ccaggcagcg accgacattt
     3661 tgcaccgggt tgcccattaa atacatcgcg ggcagtaaaa cggtgacatc gtggccggtt
     3721 aatgcgccag cggttgccag actggcggca acaccgactg cgccgcccat gctcatcaat
     3781 gcggctaaaa ggacggttgc cgcttcaccg ggcaatcccc acagtgccat tacgggttca
     3841 cagatatgtc ccacccagtc gagcaggcca gtaattttca gcgcctggat gatgacaaag
     3901 gccatcacca cgtttggcag caggttggtc gtggcgatag taaagccacg gcgcgcgcca
     3961 tcgataaaca tatccatgac gtttttgcgg acctgagttg tcatgcttgc gctccttgcg
     4021 ttgggttgcg acgttcttcg aagttaagcc agacgcgcaa aatgttggct ccgacaaatt
     4081 taaacaccag gatgacggct aaaggtacga ttacggacga gcccagaaag gcaaacaccg
     4141 cgacgccgga agagaagtag ttggtgatga tggcgctacc gctggtttga taggcggcga
     4201 atatcacttt atcacgttcg gtaatttccc cttcctgcgc cagttctttg gtcatacctg
     4261 cggcggcatc ggtgttttgc aggttagcaa tcagcgccag tgaacagatg cctggaatgc
     4321 ccaacaacgg ttttagcacg ggcgtcatta actgctgcgc ggcgcgcaat ccgccaagac
     4381 catcggtaat cgagataatt cccagagaga gaatcactga cggagccagt tccagcgcga
     4441 agagaaagcc atctttagct ccagtgccac ctgcgccgcg aaacgaggtg gtagcgccat
     4501 tcgcaccagg cagttgccca aacgatccgt tcagaacgga aaagtcaaaa acgcgccacc
     4561 aactgtctgt cccggaaaaa actccggaaa aaaagataat cgtgagaaaa aaggccagat
     4621 accctttaac acccactttt tctgtcgcca gctccccagc tacagcatcc ccttgttgtg
     4681 tcataacaat ccccattgtt tttttgtgta tgtgtgttgc ttgagccagg aaaattcctg
     4741 gagctggaaa accttatcag ccatgtgggt atgggaaaaa tgcaagtatg tttcttgcta
     4801 tgcgtttttt gcatagcgga aaaatgacgc tgtgtggcaa aggttctgcg caggattgag
     4861 ttgcagaaaa ttcataaacc cagtgacaat gacatcagta aataaaaaca gaaaattcag
     4921 ggacgaaaac gcgatggcaa aatacaacga caaagagctg gcagagacga gcaagttttt
     4981 aagttttgtc ctccgacata aaccagaggc gattggcatc gtgctggacc gtgaaggatg
     5041 ggcggatatc gataagctca tactctgtgc acaaaaagcg ggcaagcggt taacccgcgc
     5101 attactggat actgtggtcg ccaccagcga taagaagcgc tttagttatt ccagtgatgg
     5161 tacgtgtatt cgcgccgttc aggggcattc gacttcgcag gtcgccattg gcttcatcga
     5221 aaaacaccgc cacaatttct ctaccatggt acggcaagcc gttttcttga cgagataaaa
     5281 aaacaagggc tgattgcagg ggagcgccat tacgtgcatc tctctgctga tgaagcaacg
     5341 gcacgcaaag tgggagcaag acacggatcg ccagttattt taaccgtcaa agcacaggaa
     5401 atggcgaaac gaggcatccc tttttggcaa gcggaaaacg gggtctggct gacatcaacg
     5461 gttgcggttg agtttcttga gtggtaaggg aaacttccct catgaagtga ccacttgcag
     5521 cttaaataat tgcgctgtgc tgataagtgc agcaataaac aacgccgccg cgccgcagag
     5581 agtggcgctg gcaagcgccg atgttccatt gccaagcata ctggtcagcg ccgggccaag
     5641 aatttgccca atgccgtaaa tgagcgtcac aaagcctaaa agattgagat ttcccggtac
     5701 gctcagttgg cgggcgatag tcatcaccag agatgtcgtg cccataaagg tactgccaaa
     5761 accaatactg ctgatgataa gcaagagagg cgagtcgctg gcgagagtaa gcaacacact
     5821 aatagcctgc accagcaaat tcgccgtcag gcagggcaaa gccccccaac gttttgctgc
     5881 ccatagccag ccaaagcaac caggcacaat tgataagccg actaacgtcc agagatgggc
     5941 agttaacaat ggtgagcctg catctttcgc catgagcggc aggtaagtcg cgacgatgat
     6001 ataaccaaat cccgccaggc cgtacagaat agccagtaac caccagctca ttatctgttg
     6061 ctccgttttt gccaatggca ttggtgcgat ggcgtgtttt ttcgagggca ttaaaagcgt
     6121 aagtgcaatc agcatcatgc cagaaagcgc cccggcgcct tgccataacg tttgcgagga
     6181 gaggtcaaaa tgcaggcctg ccagaacata ttcattgccc agtgcgatgc caatgccaac
     6241 gccagaaaac aaagctgcca gcacaaaagg atggcgcgtg tgctgcataa tcagcgtcga
     6301 accaaaaatc agcattccgg cgctggcgac acccgccagg acgcgaatca ataacaccag
     6361 aataaatggc ggcaaccatg ccattgcgag gatcaacaac ccgctcgcca gggcagaagc
     6421 caacagaaat gggcgcaggc gcgatggctg gtgaaatgcg ccaaaagaaa atagcagact
     6481 gccagccaga taccccgcat agttgccgct ggcaatccac gagagctggc taaatgagaa
     6541 cgatccttcc gccatcatga cgggcaacat aggtgtataa aggaagcggc ctaaacccat
     6601 acctaaggtc agaaccagca tcccgaaaag cgcggtgctg aaagaaaagc gttctacggg
     6661 atgcgagggc gaaggcatgt ttaaccttgt ttgtcatttt tgctgttaac aagttaataa
     6721 taaaaaggtg aaacatgcca gcctgttaat gcgatgattg aggggtaaag cgataaaaac
     6781 gatttggttc gctaacgata taaatattac cagaggcatc catcgcaacc ccttcggcct
     6841 gtttgatgtt atgcgacaaa cctcgactgc ctttggttag tgacatttca ccgataacct
     6901 cgccaaccag cgttacttcc tggagtgcgc gagattcatg agacagcacc agaagcgtat
     6961 ttttttgttg gttaaattct gcgccggaca catcatctaa agtaaattgc cgttgtagtg
     7021 ccttgtcttt gctgatgtgc aattcattgc tacttaatag cccgttaact ttgtagacct
     7081 caatcgggtt tttctcttta aaaaaccaga aagtgtgatc ctggcgagaa taggccaatc
     7141 cttcaaaacc gcaattggtt ggggattcct gcagcgggat ttttattttt ttgaggattt
     7201 ttacttccga gtttggggtc agtgaaatca cataaatggc gtaatcgcgc tcatcgctaa
     7261 tgacaaactg attatcgcca atgtattcga tcgtctcgag atccttaaca aaatccagag
     7321 gaattgtgcg gatcaaatca ccattggtgg tcatttcaac aatggcggca ggtttgttga
     7381 tagtgctaaa aagagtattg ctttgtgccg accaggtcag cgaagagata ttattcgtta
     7441 tgcctgcaat ttctttgccg tcaatggtgg cgtggtaatt ctgaaatgat gctgcatgat
     7501 tgcttttacg ggcgcaagat tgcacgaaga aagtacacac tgccacaatg acaaaaagaa
     7561 tcacaataac gaagatgcgt ttacttaacg aaatactttt tgtcacggta atccctttaa
     7621 ggcaaatcaa taagatatcg agaagtatac gtagcgatct taatgaatcc ttaacgcgga
     7681 gaaatcccgt aatgtgcaat gcagcgccag ccaccatcct gctgacaata aactgattcc
     7741 gtatgcccag ggatcaccca ttgaatttcc tcgccaggcg gacaggtcag ccaaatgcat
     7801 aacccgcagc cgccgcgtaa atcacgggga atatcgctga cgcgaaaagt catgcccgct
     7861 gcctgcaacg ccttgcgggt ttgtatgacg ccgaccgtgg agtgaaataa aaacagatac
     7921 tctttcatgc gcgttttctc tgccgctgac cgatcaccgc cgcgccaatt gcaccagcga
     7981 attgcgcatc agggtgagtt tgtaccggca ttcccagatg gctttccagc atgtgggtaa
     8041 acctttgaca gtggctaacg ccaccagtaa acagaatcgg cgcttcacag gagagacgag
     8101 caatgaaatt ggcgctccgc cgcgccatcg cgttaatcac tccggcgaga atcgcttctg
     8161 gcgctacgcc cgcagagcgc aggctgatca cttctgattc agcaaacac
//
LOCUS       DAWDRC010000109         8203 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4253_length_8203_cov_2.456063, whole genome shotgun sequence.
ACCESSION   DAWDRC010000109 DAWDRC010000000
VERSION     DAWDRC010000109.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4253_length_8203_cov_2.456063"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2366..2375
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6687..6696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2158 a         1982 c         2029 g         2014 t
ORIGIN      
        1 attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaacgat cctttgccct
       61 ggtacaagta ttctttcgcc gcgcgcctgg ggaaaagacg tgcaaaaagg ttgtgtaaag
      121 cagtctcgca aacgtttgct ttccctgtta gaattgcgcc gaattttatt tttctaccgc
      181 aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta caaagatgcc
      241 ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt agtgaagaaa
      301 acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg tgcattgccg
      361 caaaaatatc gtgaacctgt gctggtttcc ggcactgacg gcgtaggtac caagctgcgt
      421 ctggcgatgg acttaaaacg tcacgacacc attggtattg atctggtcgc catgtgcgtt
      481 aatgacctgg tggtgcaagg tgcggaaccg ctgtttttcc tcgactatta cgcaaccgga
      541 aaactggatg ttgataccgc ttcagcggtg atcagcggca ttgcggaagg ttgtctgcaa
      601 tcgggctgtt cactggtggg tggcgaaacg gcggaaatgc cggggatgta tcacggtgaa
      661 gattacgatg tcgctggttt ctgcgttggc gtggtagaaa aatcagaaat catcgacgga
      721 tctaaagtca gcgacggcga tgtgctgatt gcacttggct ctagcggccc gcactcgaat
      781 ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca aaccaccgaa
      841 cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta cgtgaagtca
      901 gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac cggcggcggc
      961 ttctgggaaa acattccgcg cgtattgcca gataataccc aggcagtgat tgatgaatct
     1021 tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg caggtaatgt tgagcgccat
     1081 gaaatgtatc gcaccttcaa ctgcggcgtc ggtatgatta ttgccctgcc tgctccggaa
     1141 gtggacaaag ccctcgccct gctcaatgcc aacggtgaaa acgcgtggaa aatcggtatc
     1201 atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt gtggtgctta
     1261 tttccggcaa cggaagtaat ttgcaggcaa ttattgacgc ctgcaaaacc aacaaaatta
     1321 aaggcaccgt acgggcagtt ttcagcaata aggccgacgc gttcggcctt gaacgcgccc
     1381 gccaggcggg tattgcaacg catacgctca tcaccagcgc gtttgacagt cgtgaagcct
     1441 atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc gtgctggctg
     1501 gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt ttgctgaaca
     1561 ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag gcgctggaaa
     1621 atggcgatga ggagcacggt acatcggtgc atttcgtcac cgatgaactg gacggtggcc
     1681 cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat gacatcaccg
     1741 cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg tttgccgatg
     1801 gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg ccgccgcagg
     1861 gctacgctgc cgacgagtaa tgcccccgta gttaaagcgc cggctctgcc gctggcgttt
     1921 ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc tggcatagtt
     1981 ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa taaaacggag
     2041 taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt tggttatcgt
     2101 tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt gaaaggatgc
     2161 gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc ttcgctgaac
     2221 tgaagcgacg catcattatt agcgaagaac aaggttccaa ctctcattca cgccatttac
     2281 tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc ctctacaacg
     2341 agctactgct ggagatggcg cgcaannnnn nnnnngatct tcctgattaa tgaacgccag
     2401 ctctccgtca atcaacaaaa ctggctgcgt cattatttta agcagtatct gcgtcagcac
     2461 attacgccga ttttaatcaa tcctgacact gacttagtgc agttcctgaa agatgattac
     2521 acctatctgg cggtggaaat tatccgtggc gataccatcc gttacgcgct gctggagatt
     2581 ccatcagata aagtgccgcg ctttgtgaat ttaccgccag aagcgccgcg tcgacgcaag
     2641 ccgatgattc ttctggataa cattctgcgt tactgccttg atgatatttt caaaggcttc
     2701 tttgattatg acgcactgaa tgcctattca atgaagatga cccgcgatgc cgaatacgat
     2761 ttagtgcatg agatggaagc cagtctgatg gagttgatgt cttccagtct caagcagcgt
     2821 ttaactgcag agccggtgcg ttttgtttat cagcgcgata tgcccaatgc tctggttgaa
     2881 gtgttacgcg aaaaactgac tatttcccgc tacgactcca tcgtccccgg cggtcgttat
     2941 cataatttta aagactttat taatttcccc aatgtcggca aagccaatct ggtgaacaaa
     3001 ccactgccgc gtttacgcca tatttggttt gataaagccc agttccgcaa tggttttgat
     3061 gccattcgcg aacgcgatgt gttgctctat tatccttatc acacctttga gcacgtgctg
     3121 gaactgctac gtcaggcgtc gttcgacccg agcgtgctgg cgataaaaat caatatctac
     3181 cgcgtagcga aagattcacg catcatcgac tcgatgatcc acgccgcaca caacggtaag
     3241 aaagtcaccg tggtggttga gttacaggcg cgtttcgacg aagaagccaa cattcactgg
     3301 gcgaagcgcc tgaccgaagc aggcgtgcac gttatcttct ctgcgccggg gctgaaaatt
     3361 cacgccaaac tgttcctgat ttcacgtaaa gaaaacggtg aagtggtgcg ttacgcacac
     3421 atcgggaccg ggaactttaa cgaaaaaacc gcgcgtcttt atactgacta ttcgttgctg
     3481 accgccgatg cgcgcatcac taacgaagta cggcgggtgt tcaactttat tgaaaaccca
     3541 taccgtccgg tgacatttga ttatttaatg gtgtcgccgc aaaactctcg ccgtctgttg
     3601 tatgaaatgg tggaccgcga gatcgccaac gcgcagcaag ggctgcccag tggtatcacc
     3661 ctgaagctaa ataaccttgt cgataaaggc ctggttgatc gtctgtatgc agcctccagt
     3721 tccggcgtac cggttaatct gctggtgcgc ggaatgtgtt ctctgatccc caatctggaa
     3781 ggcattagcg acaacattcg tgccatcagt attgttgacc gttaccttga acatgaccgg
     3841 gtttatattt ttgaaaatgg cggcgataaa aaggtctacc tttcttccgc cgactggatg
     3901 acgcgcaata ttgattatcg tattgaagtg gcaacgccgc tgctcgatcc gcgcctgaag
     3961 cagcgggtgc tggacatcat cgacatattg ttcagcgata cggtcaaagc acgttatatc
     4021 gataaagaac tcagtaatcg ctacgttccc cgcggcaatc gccgcaaagt acgggcgcag
     4081 ttggcgattt acgactacat caaatcactc gaacaatctg aataacccta tgccaataca
     4141 cgataaatcc cctcgtccgc aggagtttgc tgcggtcgat cttggttcaa acagttttca
     4201 catggtcata gcccgtgtgg tagatggtgc catgcagatt attggccgcc tgaaacagcg
     4261 ggtgcatctg gcggacggcc tggggccaga taatatgttg agtgaagagg caatgacgcg
     4321 cggtttgaac tgtctgtcgc tatttgccga aaggctacaa gggttttctc ctgccagcgt
     4381 ctgtatagtt ggtacccata cgctgcgtca ggcgctgaac gccactgact ttctgaaacg
     4441 cgcggaaaaa gtcattccct acccgatcga aattatttcc ggtaatgaag aagcccgtct
     4501 gatttttatg ggcgtggaac atacccaacc ggaaaaaggt cgcaaactgg ttattgatat
     4561 tggcggcggt tctacagaac tggtgattgg tgaaaatttc gaaccgattc tcgttgaaag
     4621 ccgccggatg ggttgtgtca gctttgccca gctttatttc cctggcgggg tcatcaataa
     4681 agagaatttt cagcgcgctc gcatggcggc agcacaaaaa ctggaaacgt taacctggca
     4741 atttcgaatt cagggctgga acgtggcaat gggcgcttcc ggtaccataa aagccgccca
     4801 tgaagtgtta atggaaatgg gcgagaaaga cgggataatt accccggaac gtctggaaaa
     4861 actggtaaaa gaagttttac ggcaccgtaa tttcgcatcg ctgagtttac caggtctttc
     4921 cgaagagcgg aaaacagtct tcgttcctgg actggcgatt ttatgcggtg tgtttgatgc
     4981 tttagccatc cgtgaactgc gcctttctga cggggcgtta cgcgaaggcg tactgtatga
     5041 aatggaagga cgtttccgtc atcaggatgt gcgtagtcgc actgccagca gcctcgccaa
     5101 ccagtatcac atcgacagcg aacaggcccg acgggtgctg gataccacta tgcaaatgta
     5161 cgaacagtgg cgggaacagc aaccggagct ggcgcatcca caactggagg cgctactgcg
     5221 atgggccgcc atgctgcatg aggtcgggtt gaatatcaac cacagcggtt tgcatcgcca
     5281 ctccgcttat attctgcaaa acagcgactt gccgggtttt aatcaggaac agcagctgat
     5341 gatggcgaca ctggtgcgct atcaccgtaa agcgattaaa ctcgacgatc tgccgcgctt
     5401 taccttgttt aagaagaaac agttcctgcc actgattcag ctattgcgcc ttggcgtatt
     5461 actcaataat caacgtcagg caaccaccac accgccaaca ttgacgctga ttactgatga
     5521 cagtcactgg acactgcgtt tcccgcatga ctggtttagt cagaatgcgc tggtactgct
     5581 tgatctggaa aaggagcaag aatactggga aggcgtggct ggctggcggt tgaaaattga
     5641 agaagaaagt acaccagaaa tcgccgctta atgctttgcg ggccgacgag aaatgtcggc
     5701 ccgcattatt caggcacttt cgcgaatggg ttcgatttca ttcagcgtat caattaacgg
     5761 ttgcggctta ccaataagat aaccctgcat ataatcgatc cccaaagaga gcaccgcctc
     5821 gcggatctct tcgttttcaa cgtactctgc cactacccgc attttcttca ttcgcgccag
     5881 gtggcaaatc gacgccacta tctgataatc cagactattt gacacaatat tgcggataaa
     5941 actgccgtca attttaagca gatcggcatt cacattttta agccgcgcat agctggcgta
     6001 gccggtgccg aaatcatcaa tcgcaatctg gcagcctaat tcctgaagat gctgcaaggt
     6061 gatttgcgcc tgcttaacat tggtcagagc attactttcg gtgacttcaa aaataagttg
     6121 ccacgcttca atctgatatt tagccagcag ctgactgact tcaacaggaa aacgagcctg
     6181 acataccgag gttggagaca gattaatagc aaaacggtga gcgggcatct tcgctctgtt
     6241 ttcagccata aattgcagcg tatgctcaat gacccacatg tcgatactcg acgataaacc
     6301 aaattcgtgc gcgaccggta agaagctatc ggggctgatc agttcatcat tctcaccttt
     6361 catgcgcaga agaatttcat ggtagacatc accacgcata ccggtaatcg gctgggccat
     6421 caggaaaaaa tggttgtgtt ccagcgcccg ctgtagccga ttcatcatcg cgactttatc
     6481 tttcaattcg cgttgcaaat acattgctcc gcgacgctgc atattttccg gggcgttggt
     6541 cacgatggaa agttcggcca ccgtatttag ctctcccagc agcaggtaga tatgattcac
     6601 tggcgagcgc acatagcagt aactgacgcc aatctgcggt tgcatcggca ttccatccca
     6661 aaagaaacga aattgcttga gatggcnnnn nnnnnnccag tgcggtaatg cgctcctggt
     6721 gcgattctgt attcagccgc agcgcgagat cgttacccga aagctgataa acatcttcac
     6781 ccggttccaa caatggtgac agccagtgag aaagtttttg cttgtattga atccgcagca
     6841 tgatgccata attcttaacc agcatttcca tgccagggat gcgtaaataa caaagcgcag
     6901 accagggggc atcacgtaac gcgcgattca gggcgcgaac atttggcaga tgaaccaccg
     6961 ggtccacata cgcaagccgc tgaatgcgtc tgactaccgc tcgctgacgg gttgccagta
     7021 cagccatata attgacaata aaagagaata ccagataact ggaggaggtt atggtgagct
     7081 gcgtggtata gccaggataa atgggaatgt aattttgata gctgtggatg ctgatcatca
     7141 acacgaccgc ccagagcaat gaaatcagct tataaccata gcgcatcgct ccccacatca
     7201 tcaggggcag caataatgac aaggtgtaat tggtgctaaa aatcgtgctt ttttcattta
     7261 acggcatgca taacagcaat agtaaagcac ctaatgccag tagccagatt gcgaactctt
     7321 ttttggtaac tttggcatca acctgctgtt ttaattgcga ataatagcta cgtaaataaa
     7381 acggatttcg caccacccga atgatgaagt agcacagcgg gacaccaatc agattaccca
     7441 ccagcaaggc ctgataattg attaaggtcc cgaggttaaa gggcatgacg ccgaccagat
     7501 tttctctgct cgccagtaac cctacaaacg cagcaaactg gaaaagtatc agaaacagcg
     7561 ttgcaggaaa cacaatctgc cagaatagtc gctgggaaat taaacggcta tcaccatgtg
     7621 aaacgttgtt acgccggggg gtaaaaaccc tgtaaccgcc ccaacagaga atgataatga
     7681 taataaaatt agccgttaat gagagcgttt cgtaaaaacc cagctctgca tatttgcgga
     7741 caaaaatccc taaggcgatc cctggcaatg ccgcccagct gaaaaacatc atcatgctga
     7801 tcatcagtgc cagtggcaga taaaagagaa aaacttcacc ggaagaaata tgcgcgaaag
     7861 tattaatgtg ggcgaaaatg ggcaagatta aagaaggcag gaacagcgga agcccccacc
     7921 atttatcacg tatttttata taagttgcat tcagtttcat agatgctcag cagaatcccc
     7981 cacatcctga aggaggtgta ttcagacagg catcccacct gacttcgaat gatgattatt
     8041 catcactata gagagcaatg attctaagtg tcatatgaaa gttccaattg atatatatca
     8101 aacaaaataa ccctgattaa tgaattatta cgtttatcat gttaactcat cattattaca
     8161 tcatcattgt aaataattaa attaactttc ataacattaa aat
//
LOCUS       DAWDRC010000110         8188 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4267_length_8188_cov_2.396287, whole genome shotgun sequence.
ACCESSION   DAWDRC010000110 DAWDRC010000000
VERSION     DAWDRC010000110.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8188)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8188)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4267_length_8188_cov_2.396287"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3373..3382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6916..6925
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2031 a         2285 c         2018 g         1834 t
ORIGIN      
        1 cgccagcccg cgacgccaga tatttagctg cattagcagt gaagccagat gccccaccag
       61 ttctgcaatt aagccgctcg attcatcata aggtagaacc gattgccact cgccctgcgc
      121 gctctccatc gcgtagccca gcaacatgcg cgtcaggcca aaccgccagg tgtgttgacc
      181 ggtagcggga agttccagct cgcgaacgtt gtcgtcatct atcccccaac gaatgccgga
      241 ttcgttgacc cactgacgta aataacgcag cccttcttcg gtgatgtcaa accgcgctgc
      301 cagcaccggc acatccagta atgccagcac gtcttccgac acaaagcggc tgtctggcag
      361 cgataacagg ctgataaacg cttcaagtac aggatgcgac tgccgcgccc gacggtcgga
      421 aatggcgtaa ggcaggtaac gatccgcagg tgcactacca aacacagcct gaataaacgg
      481 actgtagctg tcgatatcag ccaccatcac gatgatgtcg cgcggagtaa gtgtcgggtc
      541 ttcctccagc atcgccagca ggcgatcgtg taaaacttca acttcacgct gcgggctatg
      601 gcaaacgtgg aaggtgatac tgctatccag tggatcaagc gggcgtttgt tatcgctacg
      661 ggaaaactct tcgatgttca caccagcaac ggcgcggttt tccagttcca gaatgtcaga
      721 ctgaatatta tgcagcaggt tatctggcgt cacatcgaca aaaacgtcca gctcctggct
      781 gctctccagg tcagaaagga gataaatgta gtcgcgccca agcttgcccc atgaagccag
      841 cagcgggttg ccgacatcct gttcaccatc gctgttaaag agctgcccgg cattttcgct
      901 gtcgcgaaat agcggtaatt cgcgatcttc aaaactgtgt cggcgctggc gagtcagtag
      961 tttcgccaga taagcgggat ctttaatgtc gccccagtaa taacggcagg ggttggtaaa
     1021 caggagatgg atttcaatat gtttacccag cgcctgtagc gcctggagat aaacaggcgg
     1081 taacgcggaa ataccgcata taaagacgcg cgaaggtaac cccggcgggc aggtcgtcgc
     1141 ggactccagc gtttcgataa agcgctgata gagattggcg cggtgccagc gcggttgccc
     1201 aagttcgtcg gtatattcca ccaacgcctt ccacaacggc gcttgccagg cctgtgcttc
     1261 tcccaaccct tctaccagat gtcctgtttc ccactgtgcc agccagtccg gacgatagac
     1321 cagatactgg tcaaacaggt ccgccgcttt tgaagaaagc tggaacagtt ttcgcttgtc
     1381 actatcgtca gtcagataat gccgcaacag ggtaaagtct tcgcgctcca acagttgcgg
     1441 cagcagagtc atcagtttcc agctcatgct ctgtttgtta aaggcgctct ctttggggat
     1501 ctccggtaac acccggacga acatatccca gataaagctc gctggcagcg gaaaatcaat
     1561 gtttgccgca ataccaaact tttgcgacag ggtcatttgc agccactgtg ccataccggt
     1621 actttgcacc agaatcatct ctggttcgaa aggatcgtcc agccgttcgc gttcgacaat
     1681 aaactccatc aacgcttcca gcacgtccag acgattggaa tggtagaccc ttaacatagc
     1741 ggctcctgac tactgacgat tcgggcaatg caggcgggtc atctcgcctt ctctgccccc
     1801 gggtgaaact agcgtaacgc tgatgctgac acatcccgcc tgcgatgtct gcattcggtt
     1861 gacctgccag ttagcaggtg gcgaaatcgc gcgcagttgc gtttgctgcc agccatgccg
     1921 ccagagctgt tggtactggt ttctgctggc aagactgttc attaatgttc gctggaaacc
     1981 cgataacgca gtgacaatca tcaccatcaa caccatcgcc aacattacct ccggcagact
     2041 aaagccttgt tgattcctca gggaagctga cataacgccc tctctttcag cggacaaaaa
     2101 tcgctccagc cgcgtggcga aaaaacaatg tttccatcga tgacttcgcc tgttcgccac
     2161 aacgaaacgc cttcataacc ggcaatcaat aaggcctcat tctctgccag taaacgcaga
     2221 caaacccggg caccggtggc agcatacagt aagcactgaa ctgcgggctg catctgccag
     2281 gagtgcattt ttccccactc cagcgccgac tgaacgatgg cctggcggcg caatgactga
     2341 ctttccatgc tcacgcgaga agcaaaactg cgatcctgct gactcattcc ttgcaatagc
     2401 aagctaccca aaatcagcag catcaggacc agagccagtg acgaaacacc cttttcgcgg
     2461 ttcacaggtt ggatcctgtc acgctatagc tggcatccac cacggtttgc ggatcagcct
     2521 tgctggcagc gtgcatatta accgtcaaca ccggcgagaa gccgctgaca tcctgacgta
     2581 cgacctgaaa agtgtcgata atgatggcat ccggattagt gactttatcc cagcccttac
     2641 cttcacagga tgtcgcaccg cgtagcgttt ccagcacatg ctccttcaga cgaaatccaa
     2701 tctggtcgga ctcttttacc ggttcgcgat cccagatacc attactgttc gcatcccact
     2761 gcacaatgac acagtcaccc tgtccgacaa tttccagccc ttcgcccgta cagctgccat
     2821 ggcaataacc cgccctctgg agatgcttcg cgacggtaaa tacccgcagc cagatttcat
     2881 cttccagcgc cagtttacgg gtgctcgtta aactttcacg ctgtaacgca ggcagaaagc
     2941 gtgcagcccc cagcaacaat acgctactga tcgccatagc aatcaacact tccagcagag
     3001 aaaaaccttg ctcttttaca ggcatccttc tgtttctcct tgctgacaaa gccggagtct
     3061 tccccacggc gaaaccacta gccaccactc gcccgttgag tttttgaagc gaatatgccc
     3121 agcccatgcg gtattgcgca ggccaaagaa agcaagcgaa ggtgtcaggt cgctcatttc
     3181 gacttcgggc cagcgcggca caaagaccaa tggtgaactg ccatgacagg tattcgcccc
     3241 agcaacggaa ctcacaaggc accataacgt cccctccctg ataagactga tactgtggtc
     3301 gcggttatgc cagttggcat cttcacgtaa atagagcaaa tagtcccgcg cctggctggc
     3361 ggtttgccaa agnnnnnnnn nngcgaccag cgtttcaatc agcgtataac cacgttgtgt
     3421 tttcatgccg gcagtatgga gcgaggagaa aaaaagacga gggccagttt ctatttcttc
     3481 ggcgcatctt ccggactatt tacgccgttg caggacgttg caaaatttcg ggaaggtgtc
     3541 tcgaagaatt taacggaggg caaaaaaaac cgacgcacac tggcgtcggc tctggcagga
     3601 tgtttcgtaa ttagatagcc accggcgctt taatgcccgg atgtggatcg tatccttcaa
     3661 tctcaaagtc ttcgaaacgg tagtcgaaga tggattcggg tttacgtttg ataatcaact
     3721 tcggcagcgg acgcggttcg cggcttaatt gcagatgagt ttgatccata tggttgctgt
     3781 agagatgcgt gtcgccaccg gtccagacaa aatcacccac ttccagatcg cactgctgcg
     3841 ccatcatatg caccagtaac gcgtagctgg caatgttgaa cggcaggccg aggaagacgt
     3901 cacaggagcg ctgataaagc tggcaagaga gtttgccgtc tgccacatag aactggaaga
     3961 atgcatggca cggtgccagc gccattttat ccagttcgcc tacgttccac gctgaaacaa
     4021 taatgcggcg ggaatccggg tcgtttttca gctggttcag tacagtagtg atctggtcaa
     4081 tatgacgacc atccggcgtt ggccaggcgc gccactgttt accatacact ggcccgaggt
     4141 cgccgttttc atcggcccat tcgtcccaga tggtgacatt gttttcgtgt agataagcaa
     4201 tgttagtgtc gccctgcagg aaccacagca gctcgtggat gatggaacgc aggtggcaac
     4261 gtttagttgt caccagcggg aatccatcct gcaggttaaa acgcatctga tgaccaaaaa
     4321 tggaaagcgt tccggttccg gtacggtcgt ttttctgtgt gccttcgtcg agcacttttt
     4381 gcatcagttc taaatactgt ttcatggttc ctcaggaaac gtgttgctgt gggctgcgac
     4441 gatatgccca gaccatcatg atcacacccg cgacaatcat cgggatggaa agaatttgcc
     4501 ccatgctgat gtactgcacc caggcaccag taaactgcgc gtcgggctgg cggaaaaact
     4561 caacaatgat gcgaaacgcg ccgtaaccaa tcaggaacaa gcctgagaca gctcccattg
     4621 ggcgcggttt acgaatatac aggttgagga taataaacag caccacacct tccagcagca
     4681 gctcgtaaag ctgtgatggg tggcgcggca gcacaccgta agtgtcgaaa atggattgcc
     4741 actgcggatt ggtttgcagc agcaaaatat cttctgtacg ggagccaggg aacagcatgg
     4801 caaacgggaa gttcgggtca acgcggcccc acaattcacc gttaataaag ttgcccaggc
     4861 gcccagcacc aagaccaaac ggaatgagtg gggcaataaa atcagagacc tggaagaagg
     4921 aacgtttagt acggcgggcg aagataatca tcaccacgat aacgccaatc aggcctccgt
     4981 ggaaggacat gccgccgtcc catacacgga aaagatacag cggatcggcc ataaactgcg
     5041 ggaaattgta gaacagaaca taaccaatac gtccaccgag gaagacgccg aggaagcccg
     5101 catagagtaa gttttcaact tcatttttgg tccagccact gcccggacga ttcgcccgtc
     5161 gtgttgccag ccacattgca aaaatgaaac ccaccagata catcaggccg taccagtgaa
     5221 gcgccacggg tcctattgag aaaatgaccg gatcaaactc cggaaaatgc agatagctac
     5281 tggtcatctg tcaccacaag ttcttgttat ttcgctgaaa gagaacagcg attgaaatgc
     5341 gcgccgcagg tttcaggcgc tccaaaggtg cgaataatag cacaagggga cctggctggt
     5401 tgccggatac cgttaaaaga tatgtatatg atccgcgcta taaccccccg cgaatcaacc
     5461 cgcccatgcc gcgacgctcc ataaaggctg caacctgatg gcgaacttcg gtcgccagtt
     5521 gcgcttccag actacgctgc gcaagatttt ctgcttcagc aaaatcaatg cgccgcagca
     5581 ggtattttac gcgcgctaca gaacgtccgt tcatagacag atggcgatac ccaagcccaa
     5641 tgaggattgc cacgcacatg ggatcgcccg ccatttcacc gcacaaacgg agatcgattc
     5701 catgtatttc cgcttcccgg gcgatcatcg ccagagctcg taacattgca ggatgaagac
     5761 tgtcataaat gttcgccacc cgggtattgt tgcgatcaac ggccaggatg tattgagtca
     5821 gatcgttggt gccaacagag atgaaatcga cccgctttgc cagatgcggc agcataaata
     5881 ccattgacgg cacttccagc atgatgccga tacgtggttt gggaatttcg taaccgatca
     5941 tctcctcgac ttcacgtccg gcacgttcaa tcaggcggcg tgcttcgtca acttcatcga
     6001 ggcttgtgac catcggcaac agaatattca ggttgcccgt agcggcatta gcacgcagca
     6061 tcgcccgcac ctggatcaag aagatctccg gctgatcgag cgtaatgcga atcccacgcc
     6121 aacccaggca tggattctct tcgctgatcg gcatgtaagg cagctgctta tctgctccga
     6181 catccagcgt acgcaaggtg acgggtttat cattaaacat ttgcagcatc ccctgatact
     6241 gcgccacctg ttcttcttcc gacggaaaac cactttgcag catgaatggg atttcagtgc
     6301 gataaagtcc tatgccatca atacggctgc ccagtttttc ttcatgttcc gggcttaaac
     6361 cggcattgag catgacttta atgcgttcgc cgctttttaa ctgggcgggt aaattgacgt
     6421 catcttccgc cagacggcta agctcgatct cttcactaat tagccgctga tattcttgca
     6481 gcagtaccgg ttccggatcg accagcaatt caccgcgata accatcgacg atcagcgtcc
     6541 gacgatgcag taccgaaggc tgaatatccg cgcccatcac ggtaggtatc cccagcgcac
     6601 gtaccatgat cgcagcatgg gagttggcag cgccatcgcg cacgacaaca ccgactaagc
     6661 gatcctgggg cagctcagca agcgttgtcg ctgacagttc atctgccacc agaatgaaac
     6721 gttccggcca ggcgttcggc ccttgattag cgtcatcaag atgaaacagc aatcgctgac
     6781 ccagcgcacg taaatcgcca gcccgctctt tgagatagtt atcgcttagc gcggcaaact
     6841 gttcggcaaa tttttcaatg accgttttta ccgcccactc tgccaccgag cctttatcaa
     6901 cctcggcaaa caattnnnnn nnnnngtaac gtggcatcct gccagccttc ggcaatcgcc
     6961 acaccaggtg ctgccggtaa tgcacggatt cgcgtctggc gatattgccc aaacaaggca
     7021 gtcaactgcg actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat
     7081 tcttcacttt cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac
     7141 tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttacggag
     7201 gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc
     7261 ctgccaacca ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt
     7321 tttttcagtc cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag
     7381 accgaacaga cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc
     7441 tcattcaggc gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata
     7501 attggcgtga cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta
     7561 ttttcctgca gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca
     7621 cgtttaaatg acaccacctg tctgactgga taccagtaac ttacccatcg ccagccgtca
     7681 aactctggtg tactgctggt ttgcatattg atttctgcat cgccgctcac cagctgcaag
     7741 agaaaccatt tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt
     7801 ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa ggattcggac gtctttgcgg
     7861 cttaatccta cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg
     7921 ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc
     7981 tgcccctggc gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc
     8041 accggactac ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac
     8101 ggtaaaccac tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg
     8161 tgcagagtta taaacagagg aaggggtg
//
LOCUS       DAWDRC010000111         8177 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4273_length_8177_cov_2.415907, whole genome shotgun sequence.
ACCESSION   DAWDRC010000111 DAWDRC010000000
VERSION     DAWDRC010000111.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4273_length_8177_cov_2.415907"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4769..4778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5823..5832
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1899 a         2174 c         2114 g         1970 t
ORIGIN      
        1 gacggattcg ccctgattca ccagtttggc gaggtagttt tccaccgcat ggtagggaat
       61 ccccgccatc gggattggct ctcccgctga agcaccgcgt ttggtcagtg aaatatccag
      121 cagttgcgac gcccgttttg cgtcgtcata aaacagttca taaaagtcgc ccatccggta
      181 aaacagcagg atctcgggat gctgggcttt cagcttgaga tactgctgca tcatgggcgt
      241 atgggcgtcg aaattttcta ttgtactcat ggggttaagt ccggctccct gatgttaaat
      301 gtagtgtgat ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta
      361 atcatttcag gggaagacgc gaaaggtgag ttcttgatgt cggttccgcg aaaaatgctg
      421 ctttgcatct tttgcgtgat gacgatgaaa caagatcgcc aaattcgtcg gttgaagaaa
      481 cctcctcctt cagagacata gtccccttta aaattaagtt gtaatataaa tgcaccttaa
      541 gagtatggag gcagcatgtc cggcaagcgt atctctcgtg aaaaactgac gattaaaaaa
      601 atgatcgatc tttatcaagc gaaatgcccg caggcgtcag cagagccgga gcattacgag
      661 gcgttgtttg cttacgcgca aaagcggctg gataaatgtg tgttcggcga agagaaacca
      721 gcctgtaagc agtgtccggt gcactgttac cagcctgcaa agcgtgaaga gatgaaacag
      781 attatgcgtt gggccgggcc gagaatgctg tggcgtcatc cgatcttaac cgtgcgtcat
      841 ctcatcgatg acaagcgtcc ggtgccggag ttgccagaaa aatatcgtcc aaagaaataa
      901 atctgccgga gagccatact ctccggcaaa tacggcgctt acgctttggt ggcaatcagg
      961 atggctgatg ctttgatcaa tgcaataacg cgcgagccag tggtgagttt catttcatcc
     1021 tgactttcat ttgttaccac tgccacaatt tcaaaacccg catccgtttt gatatggaca
     1081 gtggcgttca ctgcgccttc ggtgattgtc gacacgctgc cagcaaactg gttgcgagca
     1141 gagaatttca gtccacagtc ttccgttgcc agcgtgaccc acggggcttt aatcagggca
     1201 atcgcttctt tccctttcgc cagacccagc gcttgctggc tgctgtgagt cacaattgcc
     1261 accagctttg caccacctgc cagcgtcagt tctacttcat cattcacagc gcccattgct
     1321 actgcgctaa ctgttccggt taattgattt cttgctgaaa ctgccatact gcctcctttc
     1381 attgaatgtt gatcgagtat aaccacatct gttaaatcaa tgccgattta tcaattccca
     1441 gtcgcttcat ccgtgacagc agagtcgtgc gtttcagccc cagacgttgc gcagcgcctt
     1501 taggcccggc aaccacgccg tttgtttctt tcagcacgcg aacaattaac tgatattcat
     1561 cttcgccctc ctgggcgaca accgttgcgg caggcggcgt ttcaggttct ggtaaagcaa
     1621 tatctggcaa tgacagctgc agcacgttgc cgcgtgttag caataccgcg cgctcgatga
     1681 cgttttccag ctcacgcacg ttgcccggcc attccatgtt gctcaggatg cgcagcgtct
     1741 cggcaggaat gctgtcgata ttgcgcccca gacgacgggc aattttgaag gtaaaggctt
     1801 tcgccagcag cggaatatct tccggacgct cgcgtagtgg cggcaggtgg atcgggaata
     1861 cgttcagacg gtaatagaga tcgctacgga actctcggtc ggcgaccatt tttttcagat
     1921 cgcggttagt cgcggcaatt aaacgtacgt ccgtctgaat gattttgttg ctgccgagac
     1981 gttcaaactc ctgttcctgt aatacacgca gcaacttcgg ctgtaactcc agcggcatat
     2041 cgcccacttc atcgaggaat agtgagcttt tatccgccag ttcaaaacga ccaatgcgct
     2101 gagcgctggc accggtaaaa gccccacgct catgaccaaa tagatcgctt tccagcaatc
     2161 cggcaggcat cgccgcgcag ttcattttga ccatacggcg attattacgc ccactgagat
     2221 tatggatcgc gcgggcaatc agctctttac ctgtaccagt ttcaccgagg atcagaacgg
     2281 tactgtcact ttgcgccacc atttccactt gtttaagtac gctgtacatg gcttcgctgc
     2341 ggccaataat ctcgccaaat tcactatcaa cattgttgag ctgctcggtc agggcgaggt
     2401 tttcatcaac cagccgttct ttcagacgat gaatttcctg ataggcgagg gcgttatcga
     2461 cagcaattgc cacacgttcg gcaatctggc gcagtaagct cagattggta gtggtaaaga
     2521 ctttctcttc gcattgcgcc agtttcagta cgcccagcat ggtgtcgcca gacatcagcg
     2581 gtaacaggca caaggtttga atctggttgc cccaggtgtt gaacaacatg cgttcatagg
     2641 gggctaaatc gtcccgctcg tggagattaa tcaacagcat ctctttactt ttgaagacgc
     2701 gttcggtgag ggttccggct tcatcgactt cgctctgttc gtgagcggga tgctgtttat
     2761 caagatagtg agtggagtag atgttgagtt tgtttttacg gtggctgcgt aagacgatgc
     2821 tgatatcgtc aatgtcgaaa tagtaatgga tttctttggc gacttcgctg accagttcgt
     2881 ccatatccag gcgggaaagc accgcgttag tgatggcgac caggatgcgg aagttatcgc
     2941 gttcccggca taacaactca tagtcgacat tgttaacgac gcggctctgg atttgttcgg
     3001 tgacgacaga aacgatctgc gtaaatgttt gcagacgatt gaactctttt tcgctccagg
     3061 ggcgatcgtc ataacgaata aattcacagc caccaaaaat atgcccttcc gccgccagtg
     3121 gcatcaggca atagtgacca aatttggggt atagcccacc tgtggccagc tgcggccagg
     3181 tttcacaaaa ttcttcgtaa ctgcaatgca gcgtatcagg gcgcgacaaa atgctgcgta
     3241 ccggaccgtg tgccagaaca gtttcgtctt catatttaat gggggtgtct ttttcacgcg
     3301 atgcgtaata agacgcacgt tgagtctgcg cttgccacaa cacaatcgcc gcgttgtcgg
     3361 cgagcgcaga acgctttacc agttgcgaaa gagcctcaca cagcgaggcc agatcgggct
     3421 gctgcaatag tgtccgagtg atgtcgaaca acccttgctg tccgagatca ctcatcggtg
     3481 tatatgacat tttgttcttc caggaaagca ccgcaaagcg gtgcaaaagg tcattgacta
     3541 actataggag atttagaatt ttattagcat atacgcggaa gcggttcggc gtgcggtaga
     3601 tcgagggttc gtttcacgcc atacagaccg gcaagacgaa cacctttacg ttctactact
     3661 tcgccaatca gcgccgcatc ttttcccaat ggatgggaat gtaacgccgc cagcacttgc
     3721 tctgctgcgt tgcgttcaac ggcgatcacc agtttgcctt cgttggcaaa gttaagggcg
     3781 tccagtccca gcagttcgca aacgccgcgc acagcaggtt taactggcag tgccgcttct
     3841 gaaagttcaa taccacaacc gcaggctgcc gcgaactcat gaaccaccgc gtttacacca
     3901 ccacgggtgg catcacgcag cgctttcacg ccgggaatgt cacgcagcgt ctgaataagc
     3961 ggcgttagta ccgcgcagtc gctgaccagt tcgccgtcca gccccagctg ctcacgcagg
     4021 ttaaggatag tcgccccgtg gtccccgagt gtaccgctaa ccagcaatac atcgcctgcg
     4081 gttagcgtct gtgcgcccca gtgaatattc gccggaattg cgcccatgcc agcggtgttg
     4141 ataaacagtt tatctgccgc gccgcgctgc accactttag tgtcgccagt aacgatggca
     4201 atgcctgccg cgcgggcggt ttctgccatg ctggtcacaa cggctttcag cgtctccatc
     4261 ggtaatcctt cttcaaggat aaatccacag gagagataac gcggaatagc gccactgacc
     4321 gctacgtcgt tcgcggtgcc gcaaatcgcc aacttgccga tattaccgcc aggaaagaac
     4381 agcgggtcaa tgacgtaact gtcggtggag aacgccagac ggtcgccttc cgctaccagc
     4441 tgtgccaaat caagacgggc ctgatcttcc tgctctgcca gccacgggtt ggcaaaggct
     4501 tccataaaca ggctgttgat taattgctgc atcgcctggc cgccgctacc gtgggcgagt
     4561 tggatattat tcacgcttca ctctcctgct gacgatactg ataccacgcg gcgcacgctc
     4621 cttcggagga aaccatcagc gcaccaaacg cggtttgagg attacaggtg ttaccaaaca
     4681 gcgggcattg atgcggctta catttgcccg tcaagacttc gccacaacgc gcacgcgggt
     4741 catcgcagac ctgctgcggt gccgggcgnn nnnnnnnnct tcggcatcga atcgttgata
     4801 gtctggtgtt aagtgcacgc cagaagattc aatcacgcct aagccgcgcc attcactgtc
     4861 gccgttgaca cagaacacat cggcaatcgc ctgttgcgcc agcaggttac cggcatccgg
     4921 taccacccga cgatactgat tctctacctt gctgtgggcc gctattttct gctccaccag
     4981 catcaccacg ccttgaagta gatcaagggg ttcgaaacca gcgaccacca gcggacgatg
     5041 aaaatcgctg gcgataaaat tataggcatc agtgccgata accatactga cgtggcccgg
     5101 cgcgaggaac gcgtcgatac cgttatctgg ctgttccagc aaactgcgca gcgtcgggat
     5161 aagcgtaatg tgctggcaga agaagtaaaa attcttcaca tcgcgggctt tcgcctgttg
     5221 cagagtgatg gcggtggtcg gcatggtggt ttcaaaacct aagccgaaga ataccacttt
     5281 acgggttgga ttctcctgcg ccagtttcaa cgcatccatt ggcgagtaaa cgatgcgcac
     5341 atcggcaccg cgtgcttttg cctgcaacag cgacccctgt ttccctggta cgcgcatggc
     5401 atcgccaaag gtacagaaga tgacttccgg atggctggca atctccacgc aggtgtcgat
     5461 tctacccatt ggcagtacgc acaccgggca acccggaccg tggataaact caacgttttc
     5521 cggtagcaac tggtcgaggc cgaatttaaa aatggcgtgg gtatgaccgc cacacacttc
     5581 cataatccgt agagggcgtt cggcggtgta agagagatgt gaagcacgtt cgcgcagatg
     5641 ctcaattaac tgcattacct gttccggcgc gcgatattca tcaacaaaac gcattatttt
     5701 tcctcgccat acaacagcgc gccgacatct ggctcaacgt caaacatgtt ttgtaaggcg
     5761 tcgagagtgt cgcgtgcttc ggcttcatta attacgctca tggcaaagcc aacgtgaacc
     5821 agnnnnnnnn nnaaccagta cccactggcc cacgcgtggc tgaccgtttt catcgcagct
     5881 gccgactaac gttaaatcga catcgcgctg aatgccgcag acgtcgactt tcgcctggtt
     5941 gccgtcaata gtgcggatct ggccgggaac gcctatgcac atcgctgtgt ctccagccag
     6001 ttcagccact ggtccatccc ttcgccgctg gtggcggaaa taaggatgat ttcaatttct
     6061 ggattgactt cacgggcgca ggcgatgcac ttctcaacgt cgaaattgag atacggcagc
     6121 aagtcaactt tgttgagcag catcagcgag gcggcggcaa acatgtgcgg atatttcagc
     6181 ggtttgtctt caccttcggt aacggaaagc accgccactt tgtgtttttc accgagatcg
     6241 aagctggccg ggcaaacgag gttaccgacg ttttcgataa acagaatacc gttatcgtcc
     6301 agtggcaggc gcggcgcggc gtcggcaatc atctgtgcgt caagatggca gcctttaccg
     6361 gtgttcacct gaatcgctgg tgtgccggta gcgcgaatgc gcgcggcatc gttcacggtt
     6421 tgctggtcgc cttcaataac cgcgcacgga acgctgtctt tcaggcgcat taaggtttcc
     6481 gtcagcaggg tggttttacc ggaaccaggg ctggaaacca ggttgagcac cagttgcttg
     6541 cgggcagcaa agcgcgcgcg gttgcgttca gccagacggt tatttttgtc cagcacgtca
     6601 atttcgactt ccagcatccg acgctggctc atgcccggcg cgtgagtgcc cgcttcgcca
     6661 tgaccgtaat gcaggtcgcc ttcttcagtc tggctggggg taaattcagg cgctttaatg
     6721 ccggtgattt tcatcttcgg gcgtgccgcc ggggcaaatg gcgcgctacg aaacgcggaa
     6781 tgagggttat gttcatcacc ctcaatatag aggttgcctt caccgcaacc gcatgttgta
     6841 cacatcgctc actcctggtc tatttctatc cgccgaatct gtaaaccgtc gtctgccaca
     6901 atctgtagca tgtcaccatg acacagtgga cagcggcgga cgcgctgggt cagtagcgtc
     6961 acatactgtt ggcatgtttc acaccagcat tcggcctctt gttcttcgag gtgcagttta
     7021 caaccttccg ccacgctgcc gcggcaaacc agatcaaaac aaaaggcaag agagctggtt
     7081 tcgacacaag aaaatgcgcc aattttgagc cagaccccag ttacgcgttt tgcgccgtgt
     7141 tttgcggcct gctgttcgat caattccagt gcccgttggc agagggttat ttcgtgcata
     7201 tcgcctccca ttaactattg ccagctacaa gcaataattg tgccagtgtt gattatccct
     7261 gcggtgaata atgtcgatga tgtcgaaatg acacgtcgac acggcgacga aattcatctt
     7321 tagcttagaa atcgctttaa taacaatgaa ttaaaagttg gcacagaaaa tgcttaaagc
     7381 tggcatctct gttaaacggg taacctgaca atgactattt gggaaataag cgagaaagcc
     7441 gattacatcg cacagcggca tcgtcgccta caggaccagt ggcacatcta ctgcaattcg
     7501 ctggttcagg ggatcacgtt atcgaaagcg cgcctgcatc acgccatgag ctgcgcgccg
     7561 gacaaagaac tctgtttcgt cctttttgaa cattttcgca tttacgtcac cctggcggat
     7621 ggctttaaca gccacaccat cgagtattac gtcgaaacaa aagatggcga agacaaacag
     7681 cggattgcgc aggcgcagtt gagcattgac ggcatgattg atggcaaggt caacatccgc
     7741 gatcgcgaac aagttctgga acactatctc gaaaaaatcg ctggcgttta cgacagctta
     7801 tataccgcca ttgaaaacaa tgtgccggtg aatttaagcc aactggtaaa gggacaaagc
     7861 ccggtagcat gagctgaggc tttgcccgtt ttgcaggcgt tacgcctgtt tggggatggg
     7921 cgtgtcgatg agtgtcgaaa atgacatttc atcggcatgt tttcgtcaaa aatgacaatc
     7981 acctgaggaa tgcctggtga atcgttttgt aattgctgac tccacgctct gtatcggctg
     8041 ccacacttgt gaggctgcct gttcagagac gcatcgccag cacggcctgc aatcaatgcc
     8101 gcgcctgcga gtgatgctaa atgaaaaaga atctgcgccg cagctctgtc atcactgtga
     8161 agatgcaccc tgcgcgg
//
LOCUS       DAWDRC010000112         8135 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4294_length_8135_cov_2.554455, whole genome shotgun sequence.
ACCESSION   DAWDRC010000112 DAWDRC010000000
VERSION     DAWDRC010000112.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4294_length_8135_cov_2.554455"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2600..2609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4131..4140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1959 a         1906 c         2188 g         2062 t
ORIGIN      
        1 cggctcaaac ggcatttgca aatctggcgc aatgcgcatt gaccagttaa agctgtaagc
       61 gtcaccagtg tcgcggcgat tttctttcac cagcggcatc gattttttca tctgacgagc
      121 gacttcagcg gcgtcatcaa tgatgatgcg gtaatggcgg cgcgcgtttt cacccagcgt
      181 atgcacgaca aactcgtcca gtacgcggaa gtagtcggcg ctctctttcg ggccggtgag
      241 gatcaatggt aaaacctgat ctttgttggc cgggttcatt aaaattccca gcaaatacag
      301 caactcttct gctgtaccca caccgccagg gaagataatg ataccgtgag cgatacggac
      361 aaacgcttcc agacgttttt cgatatctgg catgatgatc aattcgttga ccagcgggtt
      421 aggcggttca gcggcgataa tcgacggctc tgtcatacca ataaagcgac tgtctttgta
      481 acgctgctgc gcgtgtccga ccgcagcacc tttcatcggc gcttccatcg ctcccggacc
      541 acagccggtg cagatattca gctcacgcag gcccagctgg ttcccgacgc gacgggcata
      601 caaatactcg ttttcgttaa ttgagtgacc gccccagcag accaccatat ttggcgcttc
      661 accgacatgc agcgcgcgag cgttacgcaa gatggaaaag accaggttag tgatgtggac
      721 ggagttgtcg agattcaggt tgggaaaacg aacggtgtta tgaatttgcc cataaacgaa
      781 gagaatgtca cgcagaactg cgaacaagtt ggcctgcaaa gcgcgaataa ttcgcccatc
      841 gacaaaagcc tcttccgggg gatttatcag ttccagcttt acgccgcgtt cacggcgcag
      901 gacgttaata tcgaaatttt caaaacgaga cagcaattct ttgctgttat cggtcaaact
      961 accggagttc agtacggcaa gtgaacagtt gcgaaacagt tgatagaggt cgctgctggc
     1021 ggtgcgttta agcatatcca cttccagctg cgacaacata tccatggagc caagcgggct
     1081 aatatgtgta atcaagaaaa actccttatg ggacgaatat cgtcattctc tttgattaca
     1141 atagccctgc ctgctgacct ttcacaacct tctgcgtgaa tccagcacgc aaaattgaga
     1201 aaaaatttat tgccgaacca gtcttgtggt cgatgggaca aaatcgctat tactgcgcca
     1261 cgggttaatg tccagaccgc cacgacgggt ataacgtgcg taaacgctca atttctctgg
     1321 ctggcagaag cgtaacaggt cattaaagat gcgttccacg cactgttcgt ggaactcgtt
     1381 gtgatgacgg aaagaaacca ggtaacgcag cagtttttct ctgtcaattt ggcgtccacg
     1441 atactgaatt tggatcgatc cccaatctgg ttgatgggtg atcaggcaat ttgatttcag
     1501 caggtggctg acaagcgtct cttccactac tttttcaccg ctggtggcat tctccagata
     1561 gtcagtagtg aattcatagt tatcgatagt gatatcctgg tcatcaatgc aggtgccatt
     1621 gaaatgacct atcggctggc cttccagttc atcaagacga tataacgcca cgctaacctt
     1681 accctgggcg caagtgctta agtcgcgctc cagcgtctgg cgcacctcat cccagttatt
     1741 aaaacgcgtc tggttaaaac tgttgagata gagcttaaaa ctcttcgact caatcagatt
     1801 tacgctggtg taatcaagtt caacatgacc gacagcgacc tgcggcaaac ctttcgcatt
     1861 cagccaggaa agttcataca gcgtccagat atccgtaccg tgaaaaggca ggttatccgc
     1921 tttcagaccc agcgggtcgc gattcaggct gcgtggaacg ccttgcagta ggctggcgtc
     1981 ataggtatcc cggtaatcgg ttgattttcc aagtgtcagg cccgcaagtg cctgatggtt
     2041 tgcataagaa gacatgtttc accgtcataa accagataaa ctaaacgaca agtgtatctg
     2101 gtttaagtga agagagaaat tagtggacga tttgaccgca caagccctga aagattttac
     2161 tgcacgctac tgcgatgcgt ggcatgaaga gcataaaagc tggccgttaa gtgaggaact
     2221 gtacggcgtt ccttcaccgt gcattatttc aactaccgaa gatgccgtat actggcaacc
     2281 ccagccgttt accggggaac aaaatgtaaa cgcggttgaa cgcgcttttg atattgtgat
     2341 acaacccacg attcatacct tttatacgac tcagtttgcc ggggatatgc acgcgcagtt
     2401 tggcgatatc aaactgacat tgctgcaaac ctggagtgaa gacgacttcc gtcgggtgca
     2461 ggaaaatctg attggtcatc tggtgactca gaaaaggcta aaactgccgc ccacgctatt
     2521 tatcgcgacg ctggaagaag agttagaggt catctcggtg tgtaatctct ccggtgaagt
     2581 gtgcaaagag acgctgggan nnnnnnnnnt aaacggacgc atttagcctc aaatcttgcg
     2641 gaattcctta atcaacttaa gcctcttctg taatccaaat accccatctg gttgtgagag
     2701 atctcttaca gactctgtag gagatcgcca ggaaataagc aaatacttaa aaagataaga
     2761 atcgttattc tcatttaaaa acaacatgtt gcttgttaat tcttaacttt catatgaaat
     2821 tttccttaag gcattgtctg taagcgcctt gtaagacttc gagaaaaaga cgattctatc
     2881 ttcgtcgaca gggagtcaga caacgaagtg aacatcagga tgatgacact tctgaaggac
     2941 acaccaggat ggtgtttcag ggaaaggctt ctggatgaag cgaagaggat gacgcaggac
     3001 gcgttaaagg acacctccag gatggagaat gagaaccggt caggatgatt cggtgggtca
     3061 ggaaggccag gaacacttca ggatgaagta tcacatcggg gtggtgtgag caggaagcaa
     3121 tagttcagga tgaacgattg gccgcaaggc cagaggaaaa gttgtcaagg atgagcaggg
     3181 agcaacaaaa gtagccggaa tgctgcgaaa cgaaccggga gcactgtgaa tacagtgctc
     3241 ccttttttta ttcctgctat tcttcgcggc agtttttctt attgaggttg ctttatgacc
     3301 actcatgacc gcgttcgtct ccagttgcag gcgcttgaag cgttactgcg tgaacatcag
     3361 cactggcgaa acgatgaacc cctgccgcat caatttaaca gtactcaacc gttctttatg
     3421 gacaccatgg aaccactgga gtggttgcag tgggtgctga tcccgcgaat gcacgatttg
     3481 ttagataaca accagccgtt accgggcgct tttgctgttg cgccctatta tgaaatggcg
     3541 ctggcgactg atcatccgca acgcgcgctt attttggctg agctggagaa actggatgct
     3601 ctttttgcgg atgatgcgag ctaatgctgg aaatactcta tcaggatgaa tggctggttg
     3661 cggtaaataa accctccggc tggctggttc accgcagctg gctggatcgc gacgagaaag
     3721 tagtggtcat gcaaaccgtg cgtgaccaga taggccagca tgtttttact gctcatcgtc
     3781 tggaccgacc aacttctggt gtgttgttga tgggattatc cagcgaagcc ggacggctgc
     3841 tggcacaaca gtttgaacag caccaaatcc agaaacgtta ccatgcgatt gtgcgtggct
     3901 ggttgatgga agaagcggtg ctggattatc cactggtgga agaactggac aaaatcgctg
     3961 ataaatttgc ccgcgaagat aaaggcccgc agccagcagt gacgcattat cgcggtctgg
     4021 cgaccgtaga aatgccggta gcgaccggac gttacccgac cacgcgctac ggcctggtgg
     4081 aactggagcc gaaaaccgga cgcaagcacc agcttcgtcg ccatcttgcc nnnnnnnnnn
     4141 tttgcggcac ccgattattg gcgacagcaa acatggtgat ttacgccaga atcgcagcgg
     4201 tgctgagcat tttggccttc agcggttaat gctgcatgcc agtcagttgt cattgacgca
     4261 tccttttact ggcgagccgc tggctattca cgcgggcctg gacgacacct ggatgcaggc
     4321 attatcacaa tttggctggc gcggtctgct tcctgaaaat gaaagggttg agttcagtgc
     4381 gccatcgggt caggatggag agataagttc ctaatccagg gagaaaacgg tatggcggaa
     4441 attggtattt ttgtcggcac catgtacggg aattcactgt tagtggccga agaagcggaa
     4501 gcgattctga ccgcgcaggg ccacaaagca acggtatttg aagatcctga attaagcgac
     4561 tggctgccct atcaggataa gtatgttctg gtggttacgt ccacgaccgg gcagggcgac
     4621 cttcctgata gtattgtgcc actctttcag ggaatcaaag atagtctggg cttccagccg
     4681 aatctgcgtt atggcgtgat tgcgctcggc gacagtagtt atgtgaattt ctgcaatggc
     4741 ggcaaacagt tcgatgcctt gttgcaggaa cagagcgctc agcgggttgg cgaaatgctg
     4801 ttgattgacg ccagtgagaa cccggaaccg gaaacggaat caaatccgtg ggttgaacat
     4861 tggggcacga tgttgtcctg atatgttcag cgagcggtaa atgccgtttt agcggtgctg
     4921 aatcgaatct tttcaggcaa atgccagtaa aaactgcttc atagcgcgga tttttactgg
     4981 cgtttgcctg gagtcaagcg atccatttca tactcttctt tatttcttcg ttttaaccct
     5041 tcctttcttg ttcttgtttt catttccgtg aagtggattc caccgtccag ggctaatgcc
     5101 aaaatcgggc ctcattgaac gcattaatgt tgtgttgttg cacggtgagc cgctatggcg
     5161 cgctttttat actgctattg ccagatataa acacgcgccg tattcggcga acgacctata
     5221 aaaacggcaa aaacacccta cgtcacctct gatttcctgg cgatgtcgca gtccagagtg
     5281 agcgtggcta acgcgaattt tcaggagtgc aacaatgagt tctttaagtc aggctgcgag
     5341 cagtgtggaa aaacgcacga atgctcgtta ctggatagtg gtgatgttgt ttatcgtcac
     5401 atccttcaac tacggcgacc gcgctacgct ttctattgcc ggttcggaaa tggccaaaga
     5461 tatcggcctt gatcccgtgg gaatgggcta tgtgttctct gccttctcat gggcgtatgt
     5521 tatcgggcag atccctggtg gctggttgct ggatcgtttt ggttcaaaac gcgtctactt
     5581 ctggtcgatc tttatctggt cgatgtttac cttgctgcaa ggcttcgtcg atatctttag
     5641 tggattcggc attatcgttg ccctgtttac actgcgcttc ctggtcgggc ttgctgaagc
     5701 gccatctttc cctggcaaca gccgcattgt tgcggcctgg tttccggcgc aggaaagggg
     5761 aacggcggtg tcgattttta actccgccca atacttcgca acggttatct tcgcgccgat
     5821 tatgggctgg ttgactcatg aagtgggctg gtcacacgtc ttcttcttta tgggcggtct
     5881 gggcattgtc atcagcttta tctggttgaa agtcatccac gagccaaatc aacatccggg
     5941 cgtaaatcag aaagagctgg agtacatcgc cgcgggtggc gcgctgatca atatggatca
     6001 gcaaaacacc aaagttaaag tgccgttcag cgtgaagtgg gggcagatca aacagctgct
     6061 cgggtcacgg atgatgatcg gcgtttatat cggtcagtac tgtatcaacg ccctgactta
     6121 cttttttatt acctggttcc cggtttatct ggtgcaggcg cgcgggatgt cgattctgaa
     6181 agcgggcttt gtggcttccg ttccggcggt ttgcggtttt atcggcggtg tgctgggtgg
     6241 gattatttcc gactggctga tgcgtcgcac gggttcactg aacattgcgc gtaaaacacc
     6301 gatcgtgata ggcatgttgt tgtcgatggt gatggtgttc tgcaactacg tcaacgttga
     6361 gtggatgatc atcggcttta tggcgctggc cttcttcggt aaggggatcg gtgcgctggg
     6421 ttgggctgta atggctgata ctgcgccaaa agagatcagc ggtctttccg gtggcctgtt
     6481 taacatgttt ggtaacattt ctggcatcgt cacgccaatc gcaattggtt atatcgttgg
     6541 cacgactggc tcctttaatg gggcgctgat ttatgttggt gttcatgcct taatcgcggt
     6601 actcagctac ctggtgctgg tgggcgatat caagcgtatt gagctgaaac ctgtcgcggg
     6661 gcaataagat gacgacacaa tccagtcctg ttattactga tatgaaagtc attccggtcg
     6721 ccggacatga cagcatgttg cttaatattg gtggtgcaca taacgcatat ttcacccgca
     6781 atattgtggt actcaccgat aatgccgggc ataccggcat tggtgaagcg ccgggcggag
     6841 aggtgattta tcaaacactg gtcgaggcta ttccgatggt gctgggccag gaagttgcgc
     6901 gcctgaataa agtggttcag caggtgcata aaggtaatca ggcagccgat tttgatacct
     6961 tcggcaaagg tgcctggact tttgaattgc gcgttaatgc cgtggcggcg ctggaagccg
     7021 ccttgcttga cctgttaggt caggcgctga atgttccggt ctgcgaactg ttagggccag
     7081 gcaagcaacg tgatgctatt accgttctcg gttatctgtt ttatatcggt gatcggacca
     7141 aaaccgatct tccttatctg gaaaatacgc cgggcaacca tgagtggtat cagttgcggc
     7201 atcagaaagc gatgaacagc gaagccgttg tgcgtctggc ggaagcctca caggatcgct
     7261 atggctttaa agatttcaaa cttaagggtg gcgtgttacc tggcgagcaa gaaatcgaca
     7321 ctgttcgtac attgaagaaa cgcttccctg atgcgcggat taccgttgat cccaatggtg
     7381 cctggctgct tgatgaagcc atttctctgt gcaaagggct aaatgatgtt cttacctatg
     7441 cggaagaccc gtgcggcgct gagcagggtt tctccggtcg tgaagtgatg gcggagtttc
     7501 gacgggcgac cggcttgccc gtcgcgacca acatgatcgc caccaactgg cgcgaaatgg
     7561 gtcatgcggt gatgctcaat gcggtagata ttccacttgc cgatccgcac ttctggacgc
     7621 tttccggtgc agtccgcgtg gcgcaacttt gcgacgactg ggggctgacc tggggctgcc
     7681 attctaataa ccactttgat atctctctgg cgatgtttac ccatgtgggc gcggcggcac
     7741 cgggtaatcc taccgctatc gatacccact ggatttggca ggatggccag aacctgcttc
     7801 tggacacgcc aaagattaga gatggttatc tggaggtgcc gcagggcccc ggacttggag
     7861 tgacgcttaa tatggaccgg gtcatagaag ctaaccagct ctacaaccag atgcaaagcc
     7921 acgaccgtga cgatgcggcc agtatgcagt atctgattcc aggctggaaa tatgattcaa
     7981 agaaaccatg cctggtgcgg gaataaattg ggaaagagga gaataaatga cgccgattat
     8041 cacaaaaatg gaagtctatc cggttgccgg aaaagactgt atggaattga acttgagtgg
     8101 agcacacgcc ccttttttca ccagaaatat tgtaa
//
LOCUS       DAWDRC010000113         8117 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4313_length_8117_cov_2.406847, whole genome shotgun sequence.
ACCESSION   DAWDRC010000113 DAWDRC010000000
VERSION     DAWDRC010000113.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4313_length_8117_cov_2.406847"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3648..3657
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5219..5228
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7088..7097
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7448..7457
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7875..7884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1802 a         2241 c         1897 g         2127 t
ORIGIN      
        1 ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag cagtttctca
       61 gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt aacttgttcc
      121 tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgatgcg cagcacgaat
      181 gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccggcgcgcc gttttcacct
      241 accagaccgc cgttgaagat agcgtcggca actggcttga agttcaactc ttccggcagg
      301 ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg ctctgcaccg
      361 gccagagact cggtgtcgtt gcttgccgca tcgctcactt tctgctgcag cgcgtagtac
      421 gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac ttcgtctaac
      481 gatttcactt tcgctggctg aatgtcgtcc agacgcacaa tcaggaaacc gaccgaagat
      541 ttgataacac cagacagttg gcctttttct ttcagaccag catttttcag ttcgtccggg
      601 atagtggcat cttctaacca acccatatca ccgccgttgc gggcagagat aatatcggca
      661 gatttttctt tggctaatgc agcaaaatca ccgcccttat tcagctcatc aagtaccgct
      721 ttcgcttcat cttcagtttt ggtctggatg atgctgtaac gggtacgctg cggctgggtg
      781 aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac cggttgctgc
      841 atcgtcgcgg catccagctt gatgtaactc acgcggaatt gttccggcgt catgaaattg
      901 tttttgtttt gttcgtagta gctggcaatt tcctgttcgg tcacaggctg cttcgccgcc
      961 agcgcgttaa catcgatagt cgcttcacgc accacgcgtt gttgagctac cagtgccgcc
     1021 agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt aatcagctgt
     1081 tgagtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat ccccatctgg
     1141 ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa ggctggggtc
     1201 gcgaaaatcg cctgtttaac ctgctcatcg ctgataccca gtttcagctc acgagcgtac
     1261 tgatccagaa gcgcctcgtc gatcagacga ttcagcacct gttgacgcag ggttttcata
     1321 tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg catgcgatta
     1381 cgctcgatgt tgaaggcgtt ctcgaattgc ccacggctga tttcctggtc attcactttt
     1441 gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa tatgaacgac
     1501 acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt gcgtaagctg
     1561 tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc aacgtatcga
     1621 tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac aagacgcaga
     1681 aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa cttcgtcaca
     1741 tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct gcacggaagc
     1801 tcggtacttt agcagcagcg atggtgatct ctttaccggt ctgcgggttg cggccagtac
     1861 gggcagcacg ctctttgacg gcaaaagtac caaaacctac cagtgctaca tcatcccctt
     1921 ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca gccgcagctt
     1981 tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc actcttctct
     2041 tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc agcagttata
     2101 tcaggccagc catcccctta caacacccct taataagggc aagcccgatc cgccatctaa
     2161 cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct gtttttatta
     2221 gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga cggttcattt
     2281 tgtagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc cagatcggca
     2341 attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat taacactgtt
     2401 ttaatcccgc cgcgatgcgc tgccagtagt ttttctttca aaccaccgat cggcagtacc
     2461 tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg gttaccggtc
     2521 aggcaagaaa ccagcgcggt gcacatggca ataccggcac tcggaccatc tttcggcgtc
     2581 gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg gttgatcccc
     2641 agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc ctgcatcact
     2701 tcgccgagcg aaccggtata ggtcagttta cctttccccg gaacgcacgc ggtttcaatg
     2761 gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctggcc gacacggttt
     2821 tcgttatccg cgcgcccata gtcgaaacgc tgaacaccga ggtagtcatg caggttatcg
     2881 ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac cgctttgcga
     2941 cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt gtagtaacga
     3001 ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag tgcattacgt
     3061 tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc ttcggtataa
     3121 ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt catggagttc
     3181 gacgtagcga caaacattac gtcgctgaga tcgtaatcca cttccaggta gtggtcgctg
     3241 aacgctacgt tctgctctgg atccagcact tcaagcagtg cggaggccgg atcgccacgc
     3301 atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt cacgcccact
     3361 ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg gcggtgacca
     3421 cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt acgcccggtg
     3481 gctttggcga tggactgacc cagggaggtt ttacctaccc ccggcggccc taccaggcag
     3541 aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caagatactc aaggatacga
     3601 tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgnnn nnnnnnngct
     3661 cgttcagata gtactcacgc tgggatttct ccatctgctt tttaacgcgg ttgcgaatgc
     3721 gtttctcaac ctgcagcaga tcgatttccg attccatcat tgccatcaga tattccagac
     3781 gttcgttaac gtcggacatc tccagaacgg actgtttgtc agccagtttc agcggcatat
     3841 gtgcagcaat ggtatccgcc agacgcgccg gatcgtcgat gctattcagc gacgtcagca
     3901 cttctggtgg gatttttttg tttagcttga tgtagccttc gaactggctg attgcagtac
     3961 gcaccagcac ttcctgttcc cgctcatcaa tggtcggcga ctccagatac tccgccttcg
     4021 cagaaaagtg ttcgccattg tcagagagcg cagaaatacg cgcgcgctgt aacccctcga
     4081 ccagcacttt gacggtgccg tcaggcagtt tcagcatctg caatatagag gccacggtcc
     4141 cgacagtgaa aagatcgttt acacccggct catccgttga agcttctttc tgcgcgacca
     4201 gcataatttt tttatcatgg tccatcgccg cttccagaca acggatggat ttttcccgcc
     4261 cgacaaataa ggggatgacc atgtgcggat aaaccaccac atcgcgcagc ggcaatacgg
     4321 ggatttcaat gcgttcagaa cgctcaggat tcatagagct ctctcttagt ttaatttccg
     4381 ccaggtaatc agatgacacg actgtgcttc acgccatcta ttaacatgta cgtcagtata
     4441 tggggatgtt tcccccacat tcaacgccga gaatagagga aaaattaaag gggagataaa
     4501 atcccccctt tttggttaac taattgtatg ggaatggtta attattcacc agatgcctgt
     4561 tgcgcttccg gcttaccata aatcagcaac ggtttgcttt gaccatcaat taccgactcg
     4621 tcgataacca ctttttcgac atcttccatg gacggcagat cgtacatggt atcgagcagt
     4681 gcggcttcta cgatagaacg caggccacgg gcaccggttt tacgcgccat cgctttctta
     4741 gcgatagcat ccagcgcctc gtcacggaat tccagatcca cgccttccag attaaacagc
     4801 gcctgatact gcttggtcag ggcgtttttc ggctctttga ggatctgaat cagagcttct
     4861 tcgctcagtt cattcaacgt tgcgacaacc ggcagacgac caatgaactc agggatcaga
     4921 ccaaacttga tcagatcttc cggttcaacc tgcgccagca gctcgccttc gcttgctttg
     4981 tcggacttcg cttttaccgt cgcgccaaaa ccaatgccgg aaccggtttc tacacgatgg
     5041 gaaatcactt tatccagacc ggcaaacgca ccgccacaga taaacaggat cttagaggta
     5101 tcaacctgca agaattcctg ttgcggatgt ttacgcccac cttgcggcgg aacagcagct
     5161 accgtacctt cgatcagttt caacagtgcc tgctgtacgc cttcaccgga aacgtctcnn
     5221 nnnnnnnnaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc atcgatgtag
     5281 acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tctgcaacag cttctgaatg
     5341 atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc gtcagccatg
     5401 gtgaacggaa cgtccagcag gcgcgccagc gtttcggcca gcagcgtttt accggaaccg
     5461 gtcggaccga tcagcagaat gttacttttg cccaactcga cgccattgct ggtatctccg
     5521 ttgcgcagac gtttgtagtg gttgtatacc gcgaccgcca gcactttttt cgcctgttcc
     5581 tgaccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg tagcgcactg
     5641 cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt acataaatca
     5701 acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac ttcatgctgg
     5761 cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg tttatctgtc
     5821 atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa tgccattctc
     5881 aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca cagttgcgcc
     5941 tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc accgcttcag
     6001 gggcagaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat gattgacccg
     6061 tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca cgggcatgaa
     6121 tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ctggtgaatc atcacgcgcg
     6181 aattcggcag gcagaaacgt ttaccttttg cccctgcagt cagcaagaaa gcacccatcg
     6241 aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc atggtgtcat
     6301 agatagacat cccggcagta atcaccccgc ctggagagtt aatgtacaga tagatatctt
     6361 tttctgggtt ttctgcttcc aggaacagca tctgcgccac aatcaggtta gccatgtggt
     6421 cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa tagatatcaa
     6481 aagagcgctc acctcgtgag gtctgttcaa tgaccatcgg caccagcgcc atatggggtg
     6541 caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa ttgaaaaaac
     6601 ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg ttatgggtac
     6661 ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc gaaagaggct
     6721 tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg gcggtgacgg
     6781 gcctttgtgc gaatttagcg cgttatgcta cgtaaattac gcctgctggt tcatcagctc
     6841 gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt caacagcctg
     6901 ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc tgtagaactc
     6961 gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc ctttcacgcg
     7021 ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca ggccaacaac
     7081 tacgcggnnn nnnnnnnacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt
     7141 ccagagcttg tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga
     7201 tttcgctgtc gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga
     7261 tcgcctgaga cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac
     7321 gcacttcagc gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt
     7381 cttcagtcag ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg
     7441 ctgctttnnn nnnnnnngtt ttctgcgtgg tactcttccg ggaaggtcac gtcgatggtg
     7501 aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat catacgaccc
     7561 tgacccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc gccgtcaaca
     7621 gaaccggtga agtcgatggt cacacggtct tctgcttcaa cagcgccgtc tttttctttc
     7681 caggttgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc gtcggtcact
     7741 tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac ttccggataa
     7801 acttcaaact ctacagagta ggtgaagtct tcacccagct tgtattcgcc cggaacatag
     7861 gtcggtgcgc cagcnnnnnn nnnntgagca acgatattca ttggcacttt gcctttgcgg
     7921 aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac agcggtctcg
     7981 atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt ggtttcaact
     8041 gaaacttgca tcttgttacc tccaaaaatc acagtgctcg gtcaactctt accccgcaag
     8101 cacaggttgt tggcttc
//
LOCUS       DAWDRC010000114         7976 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4394_length_7976_cov_1.995455, whole genome shotgun sequence.
ACCESSION   DAWDRC010000114 DAWDRC010000000
VERSION     DAWDRC010000114.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7976)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4394_length_7976_cov_1.995455"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2769..2778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4687..4696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5103..5112
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6530..6539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7174..7183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1727 a         2443 c         2250 g         1506 t
ORIGIN      
        1 gcggtcgggt tccggaaaat ccaccctttt acgtcactta agcggtttga ttaccggcga
       61 taaatctgtc ggtagccata tcgagctgct gggccgcact gtccagcgcg aaggccgtct
      121 ggcccgcgat atccgcaaaa gccgcgccca taccggctac atctttcaac aattcaacct
      181 ggtgaatcgc ctgagcgtac tggagaacgt gctgattggc gcgctcggca gcacgccatt
      241 ctgatgcagc aggcgaaggt cattctcgct gacgaaccca tcgcctcgct ggacccggaa
      301 tcggcacgca tcgtgatgga caccctgcgc gacatcaacc agaacgacgg cattaccgtg
      361 gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg
      421 cgccaggggc acgtcttcta cgacggctgc agccaacagt ttgataacga acgttttgac
      481 catctctacc gcggcattaa ccgcgtcgaa gagaacgcga aagctgcctg acatccccat
      541 cattgaggaa aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc
      601 agcctcagca ccctgttaaa tccggcatac gccgaagagc aggaaaaggc gctgaatttc
      661 ggcattattt caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gttcttacag
      721 gatatggaga agaagctggg cgtaaaggtc aacgccttct ttgccccgga ctacgcgggc
      781 attatccagg ggatgcgctt caataaagtg gatatcgcct ggtacggcaa cctgtcggcg
      841 atggaagcgg tggatcgcgc caacggtcag gtcttcgccc agacagtcgc ggcggacgga
      901 tcgccgggtt actggagcgt gttgatcgtc aacaaagaca gtccgatcaa caacctgaac
      961 gatctgttgg cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctccacctct
     1021 ggcttcctcg tccccggtta ctacgtcttc gccaaaaaca atatctccgc cagcgacttt
     1081 aagcgcaccg tcaacgccgg gcatgaaacc aacgccctcg ccgtcgctaa caagcaagtg
     1141 gatgtcgcca ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccggagaag
     1201 ctgaaagaac tgaaggtgat ctggaaatca ccgctgatcc ctggcgatcc gatcgtctgg
     1261 cgcaaaaatc tttccgaaac cactaaagac aagatctacg acttctttat gaattacggc
     1321 aaaacgccgg aagagaaagc ggtgctgaag cgcctgggct gggcgccgtt ccgcgcctcc
     1381 agcgacttgc aactggtgcc gattcgccag ctcgcactgt ttaaagagat gcaaagcgtg
     1441 aaggacaaca aagggctgaa cgaacaggac aagctggcaa aaaccaccgc cattcaggcg
     1501 caactggatg atttggaccg cctgaacaac gcgttaagcg caatgagttc ggtgagtaaa
     1561 gcggtgcagt aaatcgtagg tcggataaga cgcccggcgt cgcatccgac aatgtgcaga
     1621 cgttgatgtc ggatgcggtg caagcacctt atccggccta cagaccggag ccaaacatgc
     1681 aaaccatcac catcgcccca cccaagcgca gctggttctc gcttctgagc tgggccgttg
     1741 ttatcgccgt gctggtcgtc tcgtggcagg gcgcggaaat ggccccgctt acgctgatta
     1801 aagatggcgg caacatggcg acgttcgccg ccgacttctt cccgcccgat ttcagccagt
     1861 ggcaggatta cctcaccgaa atggccgtca cgctgcaaat cgccgtctgg ggcaccgcgc
     1921 tggcggtggt tctctccatc cccttcggcc tgatgagcgc tgaaaacctg gtaccgtggt
     1981 gggtctacca acctgtacgc cgcctgatgg acgcctgccg cgccattaac gaaatggtct
     2041 tcgccatgct gttcgtggtc gccgtcggcc tcggcccgtt cgctggcgtg ctggcgctgt
     2101 ttatccacac caccggcgta ctttccaaac tgctttccga agcggtagaa gcgattgaac
     2161 ccggcccggt ggagggcatt cgcgctaccg gagccaacaa gctggaagag atcctctacg
     2221 gcgtactacc gcaggtcatg ccgctgctga tttcctactc cctctatcgc ttcgaatcca
     2281 acgtccgctc ggcgaccgtc gtcggtatgg ttggcgcggg cgggatcggc gtcacgctgt
     2341 gggaagcgat tcgcggtttc cagttccagc aaacctgcgc cctgatggtg ctcatcatcg
     2401 tcacggtcag cctgctggat ttcctctctc aacggttgcg taagcacttt atctgataag
     2461 cgaggcattg atggctatgc acttgtctac acatccgacc agctacccaa cacgctatca
     2521 ggagatagcc gcaaaacttg agcaggagct tcgtcaacac taccgctgcg gcgactatct
     2581 tcccgccgag cagcaactgg cggcgcgctt tgaggtgaac cgccacaccc tgcgccgcgc
     2641 catcgaccaa ctggtggaaa aaggctgggt acagcgccgt cagggcgtcg gcgtactggt
     2701 gctgatgcgc ccgttcgatt acccgctcaa cgcccaggcg cgttttagcc agaacctgct
     2761 ggaccaggnn nnnnnnnncc caccagcgaa aaattgctat cggtgctgcg ccccgcctcc
     2821 ggtcacgtcg ctgacgcgct ggggataacc gagggggaga acgtcatcca cctgcgcacc
     2881 ctgcgtcggg tcaacggcgt cgcactctgt ttaatcgatc actacttcgc agatctcacc
     2941 ctctggccaa cgcttcaatg ctttgacagc ggttcgctac acgattttct gcgcgagcaa
     3001 acggggatta cgctacgacg cagtcagacg cggatcagcg cccgccgcgc ccaggccaaa
     3061 gagtgccagc gtcttgaaat tccgaatatg tcaccgctgc tgtgcgtgcg cacccttaac
     3121 caccgtgacg gtgaaagcag cccggcggaa tactccgtca gcctgacacg cgccgacatg
     3181 attgaattca ctatggagca ctgaatgcac gcagataccg cgacccgcca gcactggatg
     3241 tccgtgctgg cgcacagcca accggctgaa ctggcagccc gcctgaacgc gctgaacatc
     3301 aacgccgatt atgaggtgat ccgcgccgct gaaactggcc tggtgcagat tcaggcgcga
     3361 atgggcggca ccggcgaacg cttttttgct ggcgacgcca cgctcacccg cgccgccgtg
     3421 cgtctgactg acggcacact cggctatagc tgggtactgg ggcgcgacaa acagcacgcc
     3481 gaacgttgtg cgctgattga tgcgctgatg cagcaaaacc gccactttca aaacttatca
     3541 gaaactctta ttgccccgct gaacgctgac cgtatggcac gcattgccgc acgccaggcc
     3601 gaagtaaacg ccagccgggt cgacttcttt acgatggttc gcggagataa cgcatgaccc
     3661 tggaaaccgc ttttatgctt cccgtgcagg atgcccagca cagttttcgt cgcctgttaa
     3721 aggccatgag cgagccgggc gtgattgtcg ccctgcatca gctcaaacgc ggctggcagc
     3781 cgctgaatat cgccaccacc agcgtgctgc tgacgctggc cgataacgac acgccggtgt
     3841 ggctttctgc gccattaagt aacgatatcg tcagccagag cctgcgtttt cataccaacg
     3901 cgccgctggt caatcagccg gaactggcga ccttcgcggt aacggatgag gcgatttcca
     3961 gcgaacagct taacgccctt tccagcggca ccgctgttgc gccagaagcg ggcgcgacgc
     4021 tgattttaca ggtcgtcagc ctgagcggcg ggcgcatgct gcgccttact ggcgtgggta
     4081 ttgccgaaga acgaatgatc gccccgcagc tgccggagtg cattctgcac gaacttaccg
     4141 agcgcccgca cccgttcccg ctcggcatcg acctgatcct gacctgcggc gagcgcctgc
     4201 tggctatccc gcgaaccact catgtggagg tgtgctgatg tacgttgccg tgaaaggggg
     4261 cgaaaaggcg atcgacgccg cccacgccct gcaagagagc cgacgccggg gcgatactga
     4321 tttgcctgaa ctgagcgtcg cccagattga acagcagctt aacctcgcgg tagatcgcgt
     4381 gatgaccgaa ggcggtattg ccgaccgcga gctggccgcg ctggcgctga aacaggccag
     4441 cggcgataac gtcgaagcga ttttcctgtt gcgcgcctac cgcaccacgc tggcgaagct
     4501 ggcggtcagt gagccgctcg acaccaccgg gatgcgtctc gaacggcgta tttcagcaat
     4561 ttataaagac attcccggcg gccaactgct tggccccacc tacgactaca cccatcgcct
     4621 gctcgatttc accctgctgg caaacggcga aacgccgacg ctgaccacag ccgacagcga
     4681 acaacannnn nnnnnntcgc cgcacgtttt cagcctgctg gcgcgtcagg ggctggcgaa
     4741 gtttgaagaa gatagcggcg cacagccgga cgacatcacc cgcacgccgc cggtttaccc
     4801 ctgctcacgc tcctcccgcc tgcaacaatt aatgcgcggc gacgaaggct atttgctggc
     4861 gctggcctac tccacccagc gtggttacgg acgcaatcat ccgttcgcag gcgaaatccg
     4921 cagcggctat atcgacgtgt cgattgtgcc ggaagagctg ggatttgcgg tgaacgtcgg
     4981 cgaactgctg atgaccgaat gtgaaatggt caacggtttt atcgacccgc cggatgagcc
     5041 gccgcacttc acgcgcggct acgggctggt gttcggcatg agcgagcgca aagcgatggc
     5101 gannnnnnnn nngcgttgca ggcaccggaa tacggcgagc acgcaacagg tccggcacag
     5161 gatgaagagt tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac
     5221 ctcaaactcc cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa
     5281 caggagcaga accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac
     5341 caaacgcatg atccgccgcg ccatcttaaa agcggtggca atccccggtt atcaggtgcc
     5401 gtttggcgga cgcgagatgc cgatgcccta cggctgggga accggcggca ttcagctcac
     5461 cgccagcgtg attggcgaaa gcgacgtgtt gaaggtgatt gaccagggcg cagacgacac
     5521 caccaacgcc gtgtcgattc gtcacttctt taagcgcgtg accggggtga acaccaccga
     5581 gcgcacggac gatgcgacac ttatccagac gcgtcaccgc atacccgaaa cgccgctgac
     5641 cgaagatcag atagttatct tccaagtgcc gatccccgaa ccgctgcgct ttattgagcc
     5701 gcgtgagacg gaaacgcgca ccatgcacgc gctggaggag tacggcgtga tgcaggtgaa
     5761 actgtatgaa gatatcgccc gcttcggtca tatcgccacc acctacgcct atccggtgaa
     5821 ggtgaacggg cgctacgtaa tggacccgtc gccgatcccg aaattcgata acccgaaaat
     5881 ggacatgatg cccgccctgc aactgtttgg cgcggggcgc gagaagcgca tctatgccgt
     5941 gccaccgttt acccgcgtgg aaagcctcga tttcgatgat catccgttca ccgtccagca
     6001 gtgggatgaa ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct
     6061 ggatgacgcc ggaaaacgca tgtttgtctg ctccgatacc gattattgcc gccaacagaa
     6121 cgaggccaaa agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttat
     6181 gccccgggca aaggcttcag cgatgtctct tttgatttat ggccaggaga agttctgggc
     6241 attgttgggg agtccggttc cgggaagacc acgctactga aatcaatttc cgcccgcctg
     6301 acaccacagc aaggggaaat tcgctacgag aaccgttcgc tgtacgcgat gagcgaggcc
     6361 gaccgccgtc gcctgctgcg caccgaatgg ggtgtggtgc atcagcatcc gcttgacggc
     6421 ctgcgccgcc aggtgtcggc aggcggcaat atcggcgaac ggttgatggc aaccggggcg
     6481 cgtcattacg gcgatattcg tgccaccgcg cagaagtggc tggaaggggn nnnnnnnnng
     6541 agattcccgc caaccggatc gacgacctgc cgaccacctt ttccggcggt atgcagcagc
     6601 gtttgcagat tgcccgcaac ctggtgacgc atccgaagct ggtgtttatg gacgagccaa
     6661 ccggcgggct ggatgtatcg gtacaggcgc gcctgctcga tctgctgcgc ggtctggtgg
     6721 tggagctgaa cctggcggta gtgattgtta cccacgatct gggcgtcgcc cgcctgctgg
     6781 cggaccgttt gctggtgatg aaacaggggc aagtggtgga gagtgggtta accgaccgcg
     6841 tgctcgacga cccgcaccat ccgtataccc agttgctggt gtcatcggtt ttgcagaatt
     6901 gagctgatgc cggatgcggc gtaaacgcct tatccggcct acaaatgcgc tccccgtagg
     6961 tcggataaga cgcatcagcg tcgcatccga cacccgaacc acgaggcgaa aaatgattaa
     7021 cgtacaaaac gtcagtaaaa ccttcatcct gcaccagcaa aacggcgtgc gcctgcctgt
     7081 attacaaggc gcctcactca ccgtcaacgc cggcgaatgc gtggtactcc acggccattc
     7141 cggcagtgga aaatcaactc tgctacgctc gctnnnnnnn nnnactatct acccgacgaa
     7201 gggcaaatcc agatcaaaca cggtgatgaa tgggtcgacc tggtcaccgc gccagcgcgc
     7261 aaagtggtgg agatccgcaa aaccactgtc ggctgggtaa gccagtttct gcgcgtcatc
     7321 ccacgtattt cggcactgga agtggtgatg cagccgctgc tcgataccgg cgtaccgcgt
     7381 gaagcctgcg ccgctaaagc cgcgcgtctc ctcacccgcc tgaacgtgcc tgaacgcctg
     7441 tggcacctcg cgccatcgac cttctccggt ggcgaacagc agcgtgtcaa catcgcccgt
     7501 ggctttatcg tcgactaccc cattctgctg ctcgacgaac ctaccgcctc gctggacgct
     7561 aaaaacagcg ccgccgtagt ggaactgatc cgcgaagcca aagcccgcgg cgcggccatc
     7621 gtcggtatct tccatgacga aaccgtgcgc gagcaagtcg ccgaccgcct gcacccaatg
     7681 ggagtctctt catgattatc aataacgtca aactggtgct ggaaaacgag gtggtacacg
     7741 gttctctgga gatgcaggac ggcaaaatcc gcgcctttgc cgaaagccag agtcacctgt
     7801 cagaagcaat ggacggcgaa ggcggctggc tgctaccggg gctgattgag ctgcataccg
     7861 ataatctgga taaattcttc accccgcgcc cgaaagttga ctggcctgcc cactcggcga
     7921 tgagcagcca cgacgcgctg atggtggcga gcggcatcac caccgtgctg gacgcc
//
LOCUS       DAWDRC010000115         7934 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4418_length_7934_cov_2.535347, whole genome shotgun sequence.
ACCESSION   DAWDRC010000115 DAWDRC010000000
VERSION     DAWDRC010000115.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4418_length_7934_cov_2.535347"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1887 a         1996 c         2131 g         1920 t
ORIGIN      
        1 agcggctccg gtcattgctg gcattcttgc cagtatgatc gtgaactggc tgaataagcg
       61 gaagtaacgt gtcatgcggg cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc
      121 cgaaggggga aactctgcgg cctttttcgt tcttactgcg ggtaaggcac ccagtcgccg
      181 ccgttcaggc gaacgtacgg tttattctgg tattgaataa ctactgcatt tgagttctcg
      241 gagaccggtg ctgtttgtgg taacccactg gtgagttttt tccagtcaac attgtcttcg
      301 gtgaaaatct tgccatcaag aacgcgaacc accagatcgg agatagccag gaagctgctc
      361 ggttggtcga tgacaatcgg agccccctga tgtggtgcct tcatgccgaa gaatttcacc
      421 ccaacgggga cgtctgtgat agacgggcta gggatatcac gtagaccaga tatctgcatt
      481 ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga ccaccatcac tttacgaccc
      541 gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa agaatttttg cgcccgcgct
      601 ttgtaatccg ctgttttgct gacgcctgga taatggttgc cgtcatgcag tggaagcgtg
      661 ttgtagaacg tggcactacg gctgttttta tctttttcgg taacgtccag ccaacggtta
      721 agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc ccagcaaaat aaccggcaga
      781 tttgtttgat ccatcaattc gctctgcatg ccgccatttt cgcgaacttc cttcaaaaaa
      841 ccaccgaact ggccgttatg tcccatcatc aggtgctggg taaagcccag tttcgacagg
      901 ttatcaaaca gatagcagtc gttatttgcc ggttgataca gattagtgtg cgaagtctgc
      961 ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac tgtaggaggt ggcggagtta
     1021 aagttcttga actcaatatc gaaatgcgac cacagtggat gcgacatcaa tccggcggct
     1081 tcaatatccg accaggaaag cgaacagata ttaatcacca gtagttcaaa tggctgagca
     1141 tcagcgggca gcgaagacgg gaagatcgat ttacgtttcg cctccgcgtt atagaaatta
     1201 ttcagccagg cgttaaggtt tgctgttgtt ggcggtgcag tttgtgcggg tatatcaccc
     1261 actaccggtg cgccacccgt cgccgcaacg gttgccgctg cgttaccacc cgtcgttgtt
     1321 acagtggtcg tcggttgacc ggctggccac aaggagaaac ttggtcccgc cagggtaagt
     1381 acgttcagcc atagcagaat ggcaaccaca aaaacggtaa tgcgaatcca ttgtgacagg
     1441 aataaccagg ccacgaataa aacaaaaatg gccccaatca tctgccagtt aataaagcgt
     1501 gtgacaagat cgattaaata atcggtactg aaccccgcca cctgcgaacc ctggctcatt
     1561 atgctttccg ggccaggcaa ccaggtgtca tgccagaaca aagcaaagcc gatcggcagg
     1621 gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa tgggcatcag cagaaacgcg
     1681 gcaaacacca gattgaggag cggatggaag ttaagatatc ccgcccacaa caggccgaac
     1741 ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc agtattgcca gatggaagaa
     1801 ggcatggcgg tattttgcgt aaattgagtc attttttggt tgccctggct ttttccgtgc
     1861 ggcgtaacgt cccggccggt ttaacataac gaggtttagc aaagaacaaa cgtaaggtgt
     1921 cgcgaatgcg tcgcaaagag tgccgcgcca gatagcccag cgggaaaaat atcagtgcgc
     1981 aaacgacaat aatttcaatg atatcgctga tggtcatcat gatgagcgct ccacagcatc
     2041 atctaacagt cgcattggtt ctggtattcg tcgccagtgg cgaccatcgt gctcggcatt
     2101 gatgaccggt ttagaacttt gcgctaaagg cagcggcatg ccccattgtt ctggggcaag
     2161 caggcgcatc tgcaccagct cggcactgat ttgatcatct tcaaaccaga ccattcggtt
     2221 tgagaaaatg tcgccagtag gcaatgggaa aatatgattc aacgcggtat ccagatcgtt
     2281 aatccgacag aatgagagaa acagcaccag ccgattaccg ccaatggtca tgatatcgcc
     2341 ggtacggtta gggcgacata gcgtcagggc ttgctcaacg cggatacccg gtaccggacg
     2401 tagggcaacc agaacgcctt taccgtgggc aggtaataga gggttattca tcatgttgtt
     2461 gacggcatta cagaacacat cccacttctg gaaaccacgc agtttgagcg gctgggtcat
     2521 tgacagcaag gtagtgatat cttctggcac atagcgacta aacttctgcc cttgcacgct
     2581 ttcgatcatc gtcagacagc gggagagtgg cgcattccac gggataacca tatttgcacc
     2641 gcaggccaat aataaacgtt catcggtggc gcgcaggctg gcggtatttt cccgtacgag
     2701 gattttcatc gcactaccgc gctggcgacg cagagtatga atgctgcggg ccagtggctc
     2761 gatttgtgca ttttgctgta aagaaaagac caccgtcgcc gcctgagcgg tacgggcttc
     2821 attgaacagg acttcgttat tgttgaacag ttgccagtgt tccgatagcg gcggagcacc
     2881 ctccagtaca gcaacattac tcagaatgcg tttttcgtcg ctgcgtggtt ggatttccgc
     2941 ctcctggtgt tgaactaatg tccagcaacc attttgttgc tgaacgctaa gctgctgacg
     3001 ggcgctgacc cctttttcgt tgcaccagaa agcaatatcc agcaaatgtt ggtcgccctg
     3061 aaaacgcaaa ctggcaagac caaaaagtga acggtattcc tcaagtaaca atgaaaattg
     3121 tttatcgtta ttatttccgg aattaattac caaaagcgaa caatggttta gccggctcca
     3181 tttattcatt ttatccaacc atgagcgaag ccgctcggca ggaatgtttt gccatgcgtt
     3241 atttgcgcaa acaagaataa aaaggtaatt atggggatca atagaacact gcaaatcacg
     3301 ggccaaatag tatagacctt tttcatgatt tagcattgaa aataatgtta tttttcccgg
     3361 cccttgagaa tcatctaatt gaaagatttt cgctggatcg ctgtccatgc taatgaccgc
     3421 gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc gccagactga tagcatcttc
     3481 atggcgatcg acgttaaacc accagacgcc gcctgctggc atatgtcgca gctcatccca
     3541 taatgatgag ataccgatag agaatacagg gtccacaatg tccctcatgt caacattttg
     3601 tctgattatc agtttactag cgaaagctaa gaaataaacc taccattgaa attaaaactt
     3661 atcagattta gcatgtgaat caatttagca ggtcacaaga catgtgattt tagtcttttc
     3721 gtttttaact ttttctgttg cgtaacggaa agtcaaaaag tgagcacatt cccgtctcgc
     3781 cgtaaacaat aaaccgacga aatgctcaca gttagaattg tctcatgaac ggtacggtta
     3841 tttcataggg atcaagaaaa atgaataaca atgaaccaga tactctgcct gatcccgcga
     3901 taggctatat cttccagaat gatattttgg cgttaaagca ggcattttca ctgcctgata
     3961 ttgattatgc cgatatttcc caacgcgaac agttggccgc ggcattaaaa cgctggccgt
     4021 tgctggcaga gtttgcgcaa caaaagtagg ggattggtga atggccgtac tgggattgca
     4081 gggggttcgg ggaggcgtgg ggacaacaac catcaccgcc gcattagcct ggtcattaca
     4141 aatgttggga gaaaatgtcc tggtggtcga tgccagcccg gacaacttgt tgcgcctgtc
     4201 atttaacgtt gattttaccc accgtcaggg ttgggccagc gctatgctgg atggccagga
     4261 ctggcgtgac gccgggttgc gctatacctc gcagctcgat ttgctgcctt ttggtcagtt
     4321 atccattgaa gaacaagata atccacagca ctggcaaacc cggctgagcg atatttgctc
     4381 cggcttacag caactaaaag ccagcgggcg ttaccagtgg attttaattg acttaccgcg
     4441 tgatgcctcg cagataaccc accagctgct gagtttgtgc gatcactcgc tggcaatcgt
     4501 caatgtggat gccaactgcc atatccgact gcatcagcaa gcgctgccgg atggtgcaca
     4561 tattttgatt aatgacttcc gtattggcag ccaggttcag gacgatattt accagctttg
     4621 gttgcaaagc cagcgccgat tgctgccgat gctcattcat cgtgatgaag cgatggctga
     4681 atgtctggca gctaagcaac cagtcggtga atttcgcagt gatgcgctgg cggctgaaga
     4741 aatactgacg ctggcgaact ggtgcctgtt gaactattcc gggctgaaaa cgccagtcgg
     4801 gagtgcatca tgagtatcct gacccggtgg ttgcttatcc cgccggtcaa cgcacggctt
     4861 attgggcgtt atcgcgttta tcgtcgtcac ggcgcgtcgg ctttcagcgc gacgctcggc
     4921 tgtttctgga tgatcctggc ctggattttt attccgctgg agcacccgcg ctggcagcgt
     4981 attcgcgcag aacataaaaa cctgtatccg catatcaacg cctcgcgtcc gcgtccgctg
     5041 gacccggtcc gttatcttat tcaaacatgc tggttactga tcggtgcatc gcgcaaagaa
     5101 acgccgaaac cgcgcaggcg ggcattttca ggtctacaaa atattcgtgg acgttaccat
     5161 caatggatga atgagctgcc tgagcgcgtt agccataaaa cacagcatct tgatgagaaa
     5221 aaagagctcg gtcatttgag tgccggggcg cggcggttga tccttggtat catcgtcacc
     5281 ttctcgctga ttctggcgtt aatctgcgtt actcagccgt ttaacccgct agcgcagttt
     5341 atcttcctga tgctgctgtg gggggtagcg ctgatcgtac ggcggatgcc ggggcgcttc
     5401 tcggcgctga tgctgattgt gctgtcgctg accgtttctt gccgttatat ctggtggcgt
     5461 tacacctcga cgttaaactg ggacgatccg gtcagcctgg tgtgcgggct tattctgctg
     5521 ttcgccgaaa cgtacgcgtg gattgtgctg gtgctcggct atttccaggt cgtatggcct
     5581 ctgaatcgtc agccggtgcc attgccgaaa gatatgtcgc tgtggccgtc ggtggatatc
     5641 tttgtcccga cttacaacga agatctcaac gtggtgaaaa ataccattta cgcttcgctg
     5701 ggtatcgact ggccgaaaga caagctgaac atctggatcc tcgatgacgg cggcagggaa
     5761 gagtttcgcc agtttgcgca aaacgtgggg gtgaaatata tcgcccgtac cactcatgaa
     5821 catgcgaaag cgggcaacat caacaatgcg ctgaaatatg ccaaaggcga gttcgtgtcg
     5881 atattcgact gcgaccacgt accaacgcga tcgttcctgc aaatgaccat gggatggttc
     5941 ctgaaagaga aacagctggc gatgatgcag acgccgcacc acttcttctc gccggatccg
     6001 tttgaacgca acctggggcg tttccgtaaa acaccgaacg aaggcacgct gttctatggt
     6061 ctggtgcagg acggcaatga tatgtgggat gccactttct tctgcggctc atgtgcggtg
     6121 attcgccgta agccgctgga tgagattggc ggtattgctg tcgaaacggt aaccgaagat
     6181 gcgcacactt ctctgcgtct gcaccgtcgt ggctacacct ccgcgtatat gcgtattccg
     6241 caggcagcgg ggctggcgac cgaaagtctc tcggcgcata tcggtcagcg tattcgctgg
     6301 gcgcgcggga tggtgcaaat cttccgtctc gataacccgc tcaccggtaa agggctgaag
     6361 tttgctcagc gactgtgcta tgtcaacgcc atgttccact tcttgtcggg cattccacgg
     6421 ctgatcttcc tgactgcgcc gctggcgttc ctgctgtttc atgcctacat catctacgcg
     6481 ccagcgttga tgatcgccct gtttgtgcta ccgcatatga tccatgccag cctgactaac
     6541 tcgaagatcc agggcaaata tcgccactct ttctggagtg aaatctacga aacggtgctg
     6601 gcgtggtata tcgcaccacc gacgctggtg gcgctgatta acccgcacaa aggcaaattt
     6661 aacgtcaccg ccaaaggtgg actggtggaa gaagagtacg tcgactgggt gatctcgcgg
     6721 ccctacatct tccttgttct gctcaacctg gtgggcgtag cggttgggat ctggcgctac
     6781 ttctatggtc cgccaaccga gatgctcacc gtggtcgtca gtatggtgtg ggtattctac
     6841 aacctgatta ttctcggcgg cgcagtcgca gtatcggtag aaagcaaaca ggtacgccga
     6901 tcgcaccgcg tggagatgac gatgcccgca gcaattgccc gcgaagatgg tcacctcttc
     6961 tcgtgtaccg ttcaggattt ctccgacggt ggtttgggga tcaagatcaa cggtcaggcg
     7021 cagattctgg aagggcagaa agtgaatctg ttgcttaaac gcggtcagca ggaatacgtc
     7081 ttcccggctc aggtggcgcg cgtgatgggt aatgaagttg ggctgaaatt aatgccgctc
     7141 actacccagc aacatatcga ttttgtgcag tgtacgtttg cccgtgcgga tacatgggcg
     7201 ctctggcagg acagctaccc ggaagataag ccgctggaaa gtctgctgga tattctgaag
     7261 ctcggcttcc gtggctaccg ccatctggcg gaatttgcgc cttcttcggt gaagggcata
     7321 ttccgtgtgc tgacttctct ggtttcctgg gttgtatcgt ttattccgcg ccgcccggag
     7381 cggagcgaaa cggcacaacc atcggatcag gctttggctc aacaatgatg ataacgcgat
     7441 gaaaagaaaa atattctgga tttgtgcagt ggctctgggg atgagtgcgt tcccctcttt
     7501 catgacgcag gcgacgccag caacgcaacc actgatcaat gctgagccag ctgtagccgc
     7561 ccaggcggag caaaatccgc aggtggggca agtgatgccg ggcgtgcagg gcgcagatgc
     7621 gccagtcgtg gcgcagaacg gtccttcccg tgatgtgaag ctgacctttg cgcaaattgc
     7681 gccgccgccg ggcagcatgg tgctacgtgg cattaacccg aacggcagca ttgagtttgg
     7741 tatgcgcagc gatgaagtgg tgacgaaggc gatgctcaac ctcgaataca cgccatcgcc
     7801 atcattactg cctgtccagt cgcagttaaa ggtttatctc aatgatgaac tgatgggcgt
     7861 gctgccagtg accaaagaac agttgggtaa aaaaacgctg gcacaaatgc ccattaaccc
     7921 gccgtttatt actg
//
LOCUS       DAWDRC010000116         7924 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4425_length_7924_cov_2.362562, whole genome shotgun sequence.
ACCESSION   DAWDRC010000116 DAWDRC010000000
VERSION     DAWDRC010000116.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4425_length_7924_cov_2.362562"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1926 a         2077 c         1937 g         1984 t
ORIGIN      
        1 gatgaacagc accacggcca acgcatccgg caaataattc tttattttat tcatttttaa
       61 accttttaat tatattaata aaccctggtt gttggtattt ccatgtaata ggcaataaaa
      121 atcaggtaca gaggaaatat taaaaattat tgtgtgtaaa tgtctccctt taaaaagtct
      181 ggccagtttt tttcaaagta aataccgtta atacacggtt gcatacgtaa agactttact
      241 tcggtaataa aagaaccttg cgaatattga attgttaatt tgtgattttt gtcttcagcg
      301 agttcagagc gaaaacgcca ttcttctttt gcgccgcgac gagtttgtgg taggcatttg
      361 ccttgtggat caataaccat acgcgcccca cggctaccac caccagcatc aatataatgc
      421 gttaattgtg ttaataccgc ggcagaagtc agcgccatat gccgccacat aaacagctcg
      481 gcaacttcac ccacatgttt gattgccaat ccatgccgtt gcacaaattc actcaatagc
      541 agggcatcac gagtggcgcg tcggacttta tcggcatggc aaataaatcc cgcatggtca
      601 gacattcgtg cctgaatttt ttctctcaca accgacaacg gcatcccggt cccgttatcg
      661 tgcgcctgag taatgatctc tcttatagaa gccagtgccg gagctgccag ctgtgcaata
      721 tctccatcaa tattccgctt ttgcgtgcaa ccaataaagc gcgccagacg aacagcaaaa
      781 acctgcccgg cattcaacgc cgcgccgcca ggtcgggtga cgccgtgtgt gccagcaact
      841 tcccccacgg cgaaacagcc gggcagggag gtttgtcccc agatatccac ttcaatgccg
      901 ccattcatat gctgattatt catggcaaac tgcaatggct gcgtggtgag atcgtaaccg
      961 tgcatcttat acagcgagat agacagcgga ttcattcgtt gcaggcgttc gatgggcgat
     1021 ggtgccagag cgtcgttatt ttccagatac gcgcgaacgt cgtcgtccag tcgatctaat
     1081 gagaagggca ggtcacccgg caccgcttca ggattgcgat tgaaatccat aaatacctga
     1141 cgccctgatt gctgctcttg cgctactgcc atatctaaca agctggagcc aaaatccatt
     1201 acccgcgtgg cgtggaacgg ccactgatag cctttacgga aaatgtttga agccagctcc
     1261 tgggtggtgc gatagtaatc cgcgaggaag ttatactcgt tacccccggc atccacggaa
     1321 tagatatatg ggatcacctg tacataagtc ccggacaaat tccacggaaa cgtgctgcgc
     1381 ggtgtgccta taccaaactg gctttcagtc agattcgtta acgtcagacc ttcctccagc
     1441 gccagcccca gcgagccgaa acatttgtgt ggatacacac tgtcgcgata aagctcgcca
     1501 ggcccgcctg ttgccagtac cacattgggt gcagtcacaa ttgccagccc ccatgggtta
     1561 taggcgcgat gaccggtcgc gaggattgcc cccgccacac ggtcttcacc gttttcgtca
     1621 cgctgatgta gcagtttaat cactgttgca ctggtcaaca ttgggatagc aagacgctgt
     1681 acttcttcca gcagcacttt caccattaac cttgaggtac gcggcccaca cgaggttgca
     1741 cgcccggctt cgtcgtggtc ggtttgataa cgaagaatcg cgccatagcg atcttccggt
     1801 aattccagac caagatattg caacccgcca agagtgtgca gagaacctac cgcttcaaca
     1861 taagcggtgt cgtgatccat cgccccaccg ctcgccagtg cctctgccag tttggcgaag
     1921 ttgtcgccgt tgcccgcggt agcggcggta aacagtgttt gtttatcaga gccagaacat
     1981 gccgacgtcc ccatatataa cccggcggtg gcgattaaca cattttgctg gcggcgttta
     2041 agttctacag ctgcacgtag ccctgctgca ccactgccga ctaccagggc atctgttttc
     2101 cagagtgggt aatcaacacc gtctaccgtg acaacccatg cgggttctgc ctgtgcaaaa
     2161 ccctttggga aagtaacgtg tgtcagtgca tcaaggatgg cctcatcaag ctggctcatg
     2221 catcctccat cagcgtaaac aggctacttt ccatcaggcg tgggtgcttg ataattggac
     2281 ggaaggccat gaatggcaga atgtcgcgct caatctccac acccggcgcg atttcaatta
     2341 attcaacgcc ttctgccgtt aactggaata ctgcgcgttc agtgatatac aacacttcct
     2401 gaccactttg ctgtgcgtag gcggcgctaa aagtgatttt ttccacctgg gtaaccagtt
     2461 tagggatttg acctgactgg gcgatatgca aaccatctgg cgttacatca atcttgctgc
     2521 ctttggcgtc aaacgtaccg caaaacacca ctttacgggc gttttgtgca atttcgagga
     2581 agccgccggg gccaatgaga ttgccattaa gatgggagac gttgacgtta ccgtattgat
     2641 ccatctctcc catgccgagg aaggtgatat cgatgccgcc gccactgtag aattcaaatt
     2701 gatcgagtga tggaataatg gcatcggcgt tacgggcgca gccgaacaga gcatcatcca
     2761 gcatcatgcc gttatgtaca ccctgttcta cactcagcca caaactttga taaggcacgc
     2821 cttcgcgcag cgcaacccca ggaataccac cgggaatacc aaagccaaag ttggttgaag
     2881 cgcctgccgc cagttcccgc gctgcccgac gggcaatcag gcgacgcacc ggatgactaa
     2941 ccagttcaat agcgtcgtta gaacttagac ggcgatgttg accgctaatg gtcaggtcgt
     3001 aaccaccaag ataggtttgc ggttgttcgc ggtgctcaac gataccatcg accagaatcc
     3061 ccggtagctt gaccttgcga ggttcaatgg tgccagcttc cagtacatcg cgcacctgta
     3121 caaacacttt gccgccactg ttgtgtgctg ccagcgccat cgaatagcta tccatatcaa
     3181 tcgcttcttc ttcgaggcta acgttgccac gcggatcggc ataggtgccg cgcaaaatcg
     3241 cgtagtccac tttgaaaggg cgatagcgga gtttggtttc accgtcgatt tcaatcagtt
     3301 ctaccaaatc ctcggtagtg cattcgttcg acttaccgcc gccattgcgt ggatcaacaa
     3361 acgatcccag cccaacgtgg gtaaaaagtc ccggacgtcc ggcaccgatc tcccgcagca
     3421 gtgcctgaat tacgccacca gggaaacagt aggcttcaat agcattgctt ttcaccagtt
     3481 cctgcatctt gggcgaccag gtaaaatgtc cggcaataat gcgtttgagc atttcggcgt
     3541 gagcaaaacg gttagtgcct ttgctgtcgc gatcgccaat acccagcgag tggattaatg
     3601 tcagatcgcg cgggtgtccg gtttgcagga aacgctcttc aatggcggcc ataatatggt
     3661 cagcttccac cataccgccg ccgttaccgc taatggcaat ggttgcgccg tcctgaattt
     3721 gcgccgccag tgtctcggcg gttgataact tacgcataca atttccttat tgattgtgga
     3781 agctcacgcc ggctttttcg gcaaatgcgt gcgtcgcctg ttggcaatcc tgagtggcgg
     3841 acattaatcc agccagtaac tggtgggagt aatcggcgtg tttattttcc agcgccagaa
     3901 tcagttgctt aatatgtgcc agtgccaccg gaccacattt ttccagctgc gctaactggt
     3961 tcgccatcca gctttccacc tgttcttttt ctaccacttc atcaattaaa ttcgccacct
     4021 gggcttcctg cgccgtcagg cgtttaccca gcatcagcat ctggcgaccg actaccgggc
     4081 caacctgatt aagcacgcgt tcaatgccca tccagccagg caccatgccg agcatcactt
     4141 ccgggttaga gaatttcgcg ccggggcgag cgatacgaat atcgcagcac aaggcaagtt
     4201 caataccgcc accgatggta tgaccgttga gattcgcaac ggttaattgg ggtaagttgc
     4261 gcaggcgatt aaaaacgtca ttaccgcgtt taatccattt tctgcccata tctaatggcg
     4321 aataggccga ccacgattta atatcgccgc ccgcacaaaa gaatttatcg ccggttgccg
     4381 ttaattcgac taagcgtaat gccgtgtcat tttcaatttc atttaaataa tggtcgagag
     4441 attcgatcat ttcttcatta atagcatgac atttatcttc acggttcaat gtcaggcggc
     4501 aagaagccgc cgctctgctg aacagaacgg gttggtcact catgattatt ccttaacaat
     4561 aatgtgcgat gcgatcgacg ctaatatcca gcgtgctgtt aatatctttc tgccaccagt
     4621 acggtgagaa tatttccagt tcaatagcgc cattaaatcc ggcgttttca accagccgac
     4681 gaattgacgg aatattgatg acaccatctc ccggcattcc acggtcattg accagatcgg
     4741 tggtcggcac taaccagtcg gaaacatgaa acgcgaggat gcgttttccg gcacgcagga
     4801 tctgactggc gagatccggg tcccaccaga catggtagac atctaccgcc acgccgaggc
     4861 caaattcgcc atcgggatcg agttcatcac accagtccag cgcctgacgt aacgtgcaca
     4921 gacaagaacg atcggcagca gtcatcgggt gcagtggctc cagtgcaatt ggcacgccga
     4981 cctctttcga atgtggcagc aactggcgaa tcccttgttt cacctgttcc cgtgcttcgt
     5041 acagatcttt tgttcccatt ggcagaccgc caaccacctg catataacag gcggcgttca
     5101 gtacagcggc gtcgtccagc gcccggcggt tatcgtcaat cgccagtttg cgctccgcca
     5161 gcgtcggtgc ggtgtagtag gtgctacggc acaaaccgga aacgttcatg ttactggcag
     5221 caagctggcg ggcgatttgc tggagatctt cactctgcaa ctccctgcgc cagggggcaa
     5281 tcgcgccaat accgcgtgcg gcgcaggcat caataatggc gggtagcggc gttttatagc
     5341 ccagtgtcgc ggtattgata ctgaataaat ccgggcgttg taacagatct ctcatgattc
     5401 agcctcagaa gcgcggcata cttaagccgc cgtcgatggc gaccgcctga ccacaggtgt
     5461 aaatcagttt accttccgcc atcgcgcgga cagtggaagc aatatcagcc ggataacccc
     5521 aacgtcccca cggcaccaga cctttggcaa tcagttcgtc gtaataggcc gttgccggaa
     5581 tagtcatatc ggttttgatg agaccaggac gcacttcata tacgccaatt tgttcattac
     5641 aaaggcgagc agcaaacaga cgagcagcgg cagagacggc agttttggca atggtatatt
     5701 cgccgcgatt catcgccagc atgatggcgt tgatggaact gacaaaaata atcgagcggt
     5761 gcggcagttc tgcttccggt ttcggctgtg ccagtaagcg cttactgaat gcctgagcaa
     5821 ggaagaacgg tgcgcgggtg ttaatggcaa tattttggtc gaagctgtct ggttcgaggt
     5881 cgagaaggtc accgcgtttt ttcactgaaa tgcctgcgtt gttgagcagg cagtccagac
     5941 gtccccactg gttttgcgct gcgtcgagca tctcttcatg cagggagaga tcgcccacat
     6001 cagcagggaa gcaaatcact tccacgcctt ccgccagaca ttcttgttgt gtggcatgta
     6061 atttttctac gctatcggca tccggacgat cattaatcag cagattaaat ccgccacgcg
     6121 caagctccag cgcacagcct tttccaatac cacgtgcggc tccggtaatc acggcaacag
     6181 gacgagtgga tttcatacaa tagctctctt tttttgaatt ttgtttcctt agtgatgatt
     6241 agcttgcgac tggaggataa aaagatcaac catctagttg gttgatatgc gatggcgatc
     6301 acaggacatt tgataacctg atgaaagtac aagtttttgc ccttttttct gaagctgaaa
     6361 tcaaatcagg ttatgatagt caccacgaag aggtcgtggg gaagcgttat gtatcttgat
     6421 accagcaacg tacagagcct gaaagaaaag ctgctgttat gcgcggttaa tgaatttgcc
     6481 gaatatggtt acgaaggggc cagagtcgat aatatcgtca aggccgcggg gtgcagtaaa
     6541 cagacggttt atcaccactt tggtaataaa gaaaatttat ttatcgaagt actggaatat
     6601 acctggaacg atattcgaca aaaagaaaag gcccttgatt tctcagatct tccgccacag
     6661 aaagccattg agaaaataat cgattttaca tgggattatt atatttataa tccctggttt
     6721 ttaaaaattg tgcatagtga aaatcaaagt aaaggtgtgc attatgcaaa atcacagcgt
     6781 cttttagaaa ttaaccatgc gcacttgcag ttaatggaat ccttattgga tgaagggaag
     6841 aaacaaaata tttttaagcc agatatcgac ccattgcagg tgaatattaa tattgctgcg
     6901 cttgggggat attatttgat caaccagcat acgcttggcc tggtttatca catcagtatg
     6961 gtttcacccc aggcactgga agccagacgt aaggtcatca aagagacgat ccttagctgg
     7021 cttttggttg acccttcatc taccgcgcgc gaataaaact gccatccggc tggcgggtaa
     7081 acaggacttg ttgattattc cccgtatcaa tggttaagcc cgtcaccacg ccgctggcgt
     7141 tctggcggat tttcaccatc tgcccgtttt gcaaattact cagtggctta tccgcgcctt
     7201 ctacctgcgc catggcatag acatcggtag cgggcagtcc gtggtcgcgg aacagctgcg
     7261 ccatcgtttt accgggttcg acacgatagg agcgccattg attatcaatt ccgctatctg
     7321 gctggaatgg ttgcgtatgg ggggtttgcg gctgctcttc tggttggcct tcctgtattg
     7381 gttctggagc gaccggagcc acctgatcag gatcattttg tggcgtgact aattgcgccc
     7441 gcagctgttc ttctgtcggt ggttgtgatt gcgactgaat atctaactgc gcttcacgcg
     7501 tcaccaccgg cgcgttgggc gtatcatcag atggaagcag aaatccgacc accaatacaa
     7561 tggcggcaat aatgatcccc cgacgatgca tcggcggcag cgggtccatg aagcgaaaat
     7621 tgtccggcgc gtgccagact ttctccaggg ttggttttag ttcaaagcgc ccgggcatgg
     7681 tttcctcctg ctccgcgtct tgttcctcaa tcatagccta tgaataagct aacgctatga
     7741 tgtccgtggt aaacccgcct ttattatatt catacgggat attgctattg tttctttttc
     7801 cccgggattt gtcatcattc ccgcgacaaa gttttaccca aagaagtgtg gctgatatgc
     7861 tgcccgctac tttataccct aagaaaggaa atacgatgac caccccaact tttgacacca
     7921 tcga
//
LOCUS       DAWDRC010000117         7901 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4446_length_7901_cov_2.452332, whole genome shotgun sequence.
ACCESSION   DAWDRC010000117 DAWDRC010000000
VERSION     DAWDRC010000117.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4446_length_7901_cov_2.452332"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1722..1731
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1842 a         2210 c         1872 g         1967 t
ORIGIN      
        1 cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt acggctttca actttacgct
       61 gggcgttggc aatcgctttg gtcacccacg ggtgctcaat ggcttcgcct ggcttcatac
      121 ccagtttacg catcatgccg gatactcggt cggaagcaaa aatacgcatc agcgcatctt
      181 ccatcgacag gtagaaacgg gaagaaccag catccccctg acgaccagaa cgaccgcgca
      241 gctggttatc gatacgacgg gattcgtgac gttcagtacc gatgatatgc aggccacctg
      301 ctgccagtac cgcatcgtga cgtacctgcc agtcggcttt aattttttca atttgctctg
      361 cagtcggatt ttccagcgcg gcaacttctg cctgccagct accaccgagc acaatatcgg
      421 taccacgacc cgccatgttg gtcgcgatag tcaccgcagc cggataacct gcctgagcaa
      481 caatcgccgc ttcgttggca tggaatttgg cgttcaggac gttgagctta ataccggctt
      541 tggtcagttc atttgacacc agctccgatt tttcgatgga gattgtaccc accagcaccg
      601 gctggccttt cgcagtacgt tctttgatat cttcaatgat cgcctgaatt ttttccgctt
      661 cagtcatgta gaccaggtcc ggcagatctt tacgaatcat tggacggttg gtcggaacaa
      721 cgacagtatc cagcttatag atggagctga attcgaaagc ttcggtatca gcagtaccag
      781 tcatccccgc cagtttttca tacagacgga agtagttctg gaaggtgatc gaagccagcg
      841 tttggttttc attctggatc tgcacacctt ctttcgcttc cacagcctgg tgcagaccat
      901 cagaccagcg acggccctgc atggtacgac cggtgtgttc gtcaacgatg ataacttcac
      961 catctttaac gatgtagtcg acgtcgcggg taaacagcgc atgagcgcgc agcgccgccg
     1021 ttacgtggtg catcagcatg atgttggccg gagagtacag agactcacct tcatccatga
     1081 tgccttcttt aaccagcagt tcttcaatca gaaccagacc acgttcggtc aggttcacct
     1141 ggcgagattt ttcatccacc gagaagtggc cttcgccctg gaaggtttcg gagtcttctt
     1201 tttcctgacg gatcaggtgc ggaataattt tattcacgcg tttatacatt tccgagctgt
     1261 cttctgctgg gccggaaatg atcagcggtg tacgcgcttc atcgatgagg atggagtcca
     1321 cttcgtccac cagcgcatag tgcagtttac gttgtacacg ttcttcaggg ctgaatgcca
     1381 tgttgtcgcg caggtagtca aagccgtatt cgttgttcgt accgtaagtg atgtcagcag
     1441 cgtaggcttc acgctttgcc ggtgctggca tgcccggcag gttgataccg acagtcaggc
     1501 caaggaattc aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg tagtcgttga
     1561 cggtaactac gtgtacgcct ttaccggtta gtgcgttcag gtaagcaggc agcgttgcgg
     1621 tcagggtttt accttcaccg gtacgcattt cggcgatgca gcgttcgtta agaaccatac
     1681 cgccgagtaa ctgaacgtcg aagtgacgca tgccaaagac gnnnnnnnnn ngcgcttact
     1741 tgcctcacgc accacggcga aagcttccgg gatcagattt tccagcactt cgcctttttc
     1801 cagacgcgca cgaaactctg cggttttccc tttcagttct tcgtcggaga gtttttccat
     1861 ctccggttcc atggcattga tgatgttgac cactttgcgc atccggcgca gggtgcgatc
     1921 gttacgacta ccgaaaactt tagttaataa tttgattagc ataataaaat ctcaaacgcc
     1981 ccgcgttgcg gagttaataa aatgaggtaa aggtttattg ttgttagctg aggcgttgag
     2041 ggccagcacg gatgccttgc gcctggctta tccagacggg cgtgctgaat tttgcttgtg
     2101 gggtaaaatg cgcataatca atgcgataac ccttttcaga cggcgtgcct tcctgggtca
     2161 gaagcgcgct aagcgtatcc agtaatgcaa gatgttgcgc ctgaagaggc aaagattctt
     2221 cagcaacggg cagcgtttgc ggtgccatcg cgaaggaaag atggcggatt accgtgcgaa
     2281 tggcatgttg atgccagtaa tcaacggaat agttcgaatt cgggcggcgc gtgttcgctt
     2341 ccagcaaggc caattgacca aagttaactt tggctgaagg ctcgtggttg cgggttgtcg
     2401 cttttgcggg cgcgtttggt tcggcggcat tgctgagtgc aggcaaacct aaactcgccg
     2461 cgaccatccc taataagaga tgcggccaga agtagcgttt accaaactgt cgccagcgcg
     2521 tcagtattcc actcacgtta ttgccatccc gatcgtattc aaacgtattt agcgcacaaa
     2581 tcttatcatt aaagcctgaa gtccacagca cgaatgaaga taagttgtgc gtaaaagtgc
     2641 ttaagaaaac agcgttcgca ccgtttttta tagctcttat aaaattcaaa aaaaaacgac
     2701 tggttcacct ggccggagag agtgccagat ttgccagtcg aattttatac gacagtataa
     2761 atgtcgttat gccagtacgg ctgaaggcgc tttgaaggcc aacggcagtt ctgcgtcgtc
     2821 ctggaaggtc acatattccc aggcttcctg tttcgccagg acagcctgca gcagtttgtt
     2881 attcagtgca tgaccggatt tataagcggt aaatgcacca ataatattgt gaccacacat
     2941 gaacaagtca ccgatcgcat cgagcatttt gtgacgcaca aattcgtctt caaaacgcag
     3001 gccgtcttcg ttcagtacgc gataatcgtc aacaacgatg gcacaatcga agctgccgcc
     3061 caggcacaaa ccacgggact gcagatattc gatatcacgc atgaaaccga acgtacgcgc
     3121 acggctgatc tggcgcataa acgcatcagc ggagaagttc atcgcatagc gctggttgct
     3181 ggaatcaata gccggatggt taaaatcgat ggtgaaatcc agagaaaaac cattgtacgg
     3241 cttaaattca gcccacttat cgccatcttc gacacgaaca gtttctttaa tgcgaacaaa
     3301 tttcttggcg cagttcaact cgtcgatacc ggcgtcaagc agcaggtata caaacggagc
     3361 ggcgctgccg tccatgatcg ggatttccgg cgcgttaact tcgataacaa tgttatcgat
     3421 gcccaggccc gcgagagcag cattgaggtg ctctacggtt gaaatccgca catcatgctc
     3481 gttgaccaga cacgtacaga gcatggtatc acgcacagat ttggcatcgg ccgggaaatc
     3541 taccggtgga ttcaagtcgg tgcgacgata gatgaccccg gtgttggccg gcgcagggcg
     3601 taacgtcagg gtgactttct tgccggtatg taaaccgaca cccgtcgcct gaacgatacg
     3661 tttaagtgtc ctttgtttga tcatcgtatt atctcgccaa attacctatc caaccgaagt
     3721 gtactataca ttcggcgggc cagtttagca caaagagcct cgaaacccaa attccagtca
     3781 attcttaatc agcttgctta cgcaggaatg ctgggatatc cagataatcc ggctctttcg
     3841 cagtttgcgg cgcattgtca ttcacgactt tagcaaccgg cttctgctcc tgggtcagcg
     3901 gagccatccc gtgctgctgg tagcgatcca tcactggctg ctgaacctgc ttattggtca
     3961 ccagagtgat ttcaggacgt ttgtccatgc cgatacctgt cgcaacaaca gttacgcgca
     4021 gctcgtcatt catatccggg tcaagagaag taccgataac cacggtcgcg ttgtcggaag
     4081 caaatgcacg gatggtgtta cctaccgttt cgaactcatc cagacgcagg tcgaagcccg
     4141 ctgtgatgtt aaccagcacg ccgcgtgcgc cagacaggtc gatatcttcc agcagcggag
     4201 aagagatagc catttcagca gcttcttccg cacgatcttc accgctcgcc acaccagaac
     4261 ccatcattgc gtagcccatt tcagacatta cggtgcgtac gtctgcaaag tccacgttca
     4321 tcaaacccgg acgagtaatc agttcggcga taccttgcac agcgcctttc agtacatcgt
     4381 tcgctgcgcc aaacgcatcc agcagggaga tacctcggcc cagaactttc agcagtttgt
     4441 cgttcgggat agtgatcaga gagtccacat gcttggacag ttcagtgatc ccctgctccg
     4501 cgaatgccat acgcttcttg ccttcaaagt tgaaaggctt agtgacgaca gcaacggtca
     4561 ggatacccaa atcttttgcc acttcagcga cgactggtgc tgcacctgta ccggtaccac
     4621 cacccatacc cgcagcaata aagaccatgt ctgcaccttc cagcgccgca cgcagtgcat
     4681 cgcgatcctc atcagccgca ttgcggccaa cttctggatt agcgccagcg cccagtcctt
     4741 tggtgatacc gctaccgatt tgaatcgtct gtccaaccgc tgttttacgc agcgcttgtg
     4801 catcggtatt taccgcgaag aattcaacac cttcaatgcg ctcacgcacc atgtgttcaa
     4861 cagcattacc gccgccgccg ccgacgccga tgactttaat caccgcgtca ttggtaagtt
     4921 ccattggttc aaacatagtt tctctccgat ttgtgcctgt cgcctgaggc cgtaatcatc
     4981 gtcggcctca taaaaattaa aactcttttc gcagccaact attgagtcgc ttgatccacg
     5041 agccaactga tgctgtaaca cgtttttcta cttcagcttc accgttaaga tgtgactctt
     5101 tcccatagtg aagcaatccc accgccgtcg aataatacgg ctcctgagca taatccgtta
     5161 aaccggtaat gttcagcggc gcgccgatac gcacttgcgt atgaaacacg cgctgagcac
     5221 aggctgcaag accttcgatc tgcgctgcgc caccggttaa tacaatgcct gccgccaggt
     5281 gatgtttaac cccttgttgg cgaagctttt cctgcaactg caatatctct tcgttgacca
     5341 gattgagcag ctcggtatag cgcggctcga tcacctctgc cagggtctga cgttgtagac
     5401 tccgcggcgg acgaccgcct acgctcggca cttctacgct ctcatctttt ccaacgatgg
     5461 agcccagcgc acaaccgtgg cgaactttaa tcgcttcggc gtcacttggc ggtgtgccaa
     5521 aggcgtaagc gatatcactg gtcacgacat tgccagcata aggaattacc ttcgtgtggc
     5581 gcaatgcccc accggtataa acggcgatat ccattgtacc accaccgata tcaacgacgc
     5641 agacacccag ttcacgttca tcttccgtca ataccgaata acttgatgcc agaccggcaa
     5701 atatcagttg gtcaactttc agcccacaac gttcaaccgc tttgacgatg tttttcgcca
     5761 tatcattgtg acatgtgatc aggtgcactt ttgcctgcat ccgcacgccc gaaagcccta
     5821 ccggattctt gatcccttcc tgatagtcaa tcgcatactc ttgcgggatc acatgcaaca
     5881 cacgatgctc atcgcgcaca cgcaccgatt tcgcggtatg gacgacgttt tccacatctt
     5941 cttgcgtcac ttcttcttca gaaataggca ccataccaat ttcattctgg cagctgatgt
     6001 gcttaccaga aagcgccaga tataccgaag agatctgaca atctgccatc aattctgcct
     6061 ggtcaatggc gcgttgtacg cacttgacca cggattcgag gtcgttcacc ccacctttat
     6121 ccataccacg cgacgggcaa ctacccacgc caatgatatt gaccataccg tcgggcagaa
     6181 cttcccctac taaagcggca accttcgcgg taccaatctc cagtcctact accagttttc
     6241 tgtccgtcgc cttgatcatt gttgttctgc ctgtgcctga ttttgttgct gagtagattc
     6301 ctctggcggc aagggcgccc agcctactgc cgctccagag tcataacgca aatcaacgta
     6361 gctaatccgt ttgccatcgg tttgcgcctg ctgctgtaaa accggataaa gttctacaaa
     6421 gcgagccaaa cgtttcatcg tatcgccccg accaagattg agcttaatat cgttattcag
     6481 cgtcaactgc caggaacgtc gcgcggtcat cgccgcttcc ttcagagtaa atctgtcctt
     6541 tgccagcatc tgccccattt cgcgatagcc ctgcaacact tcattggcgc tgccttccgg
     6601 gccatacagc attggaagca cctgcttgct ggtgcgatct ggcggcacgc tgaaggtgtt
     6661 tccttccgcg tctaccatat gttgatcatt ccaccgcgca atcggcacat attcaaccag
     6721 atgaatcttc aattcatcag gccactgctt tctgacgctc acctgcttaa tccacggcag
     6781 gcgttgttct atttgcgtct ggatgatgtt gacatcctgg gtcataaagg tacctggctc
     6841 acccaatgcc aggatcgact gccggatatc gtcattacgc gtgtaatggc gttcaccggt
     6901 caacaccagc tttgagagcg gcaggcgttg cgcatcttcc atccagccca acacgaccca
     6961 gccgctcacc aacactgtcg ttaaaacggt cagcaggaaa aggatccccg ccagacgcgt
     7021 tccattattg cggcgagaag aaacctcttc ttcgctattt cgcgtgttca gagcagcctg
     7081 cgacatatta gtccgccagt tccagaattc gtactaccaa ctgcgagaag ctcatacctg
     7141 cctgacgtgc cgccatcggc accagactgt ggctggtcat acccggtgag gtattggctt
     7201 ccagcagata aaactgtcca tcactgtcca gcataacgtc aatacgtccc catcctttgc
     7261 aacctaacgt cgtccatgct tttagcacta atgcctgcaa attggcctct tgtgacgctt
     7321 ccagacctgc ggggcagaaa tactgtgtct catcagagag atacttcgcc tcataatcat
     7381 agaaggttcc ggccggttga atacgtattg acggtaaaat ttcttcaccg agtatcgcaa
     7441 ccgtgaactc cggcccactt agccattttt caatcaatac ttcttcatcg tgctgaaatg
     7501 ccaatcttaa tgcatcttgt agagcatttt ctgctactac ttttgacatt cccacactgg
     7561 aaccttcgcg gctcggctta acgataaccg gcaaacccag agaagaaatt tctgctaact
     7621 gcttatcgtt aaggcctttt tcaaactctg cgcgggttaa cgctacccac ggcgcgaccg
     7681 gtaaaccggc accctgccac agaagtttgc tgcgtagttt atccattgaa agcgcagatg
     7741 ccatcactcc gctgccggta taaggcaagc ccatcagctc gagcatgccc tgcagcgtac
     7801 catcttcacc gccgcgaccg tgtagcgcga taaacacttt ctgaaaaccc atcgacttca
     7861 gttgcgtcac gtcgacttct ttcgggtcga caggatacgc g
//
LOCUS       DAWDRC010000118         7732 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4554_length_7732_cov_2.307412, whole genome shotgun sequence.
ACCESSION   DAWDRC010000118 DAWDRC010000000
VERSION     DAWDRC010000118.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4554_length_7732_cov_2.307412"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4322..4331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5485..5494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6976..6985
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1754 a         2041 c         1901 g         2006 t
ORIGIN      
        1 acatgcacct tcacaaagac ggtcctgtgg acataccctg ccgcagattt cgggtaagga
       61 actggtctgg tggcaaagtt ctgccgcttc aataatcttt ccttcctgta ccagacggat
      121 gtaatccgga atagcgttat gcagcgggca atgccagttg cagttagctt tttcggcaca
      181 ataaacacag cggtcactct cataagtcgc ttgttgtgga tccagcccgc aatagatttc
      241 gccaaagtgg gttttccgtt cactcgctga aattttatct gcgcctttac gcgagttaac
      301 ggggagcaac gctgcactgc gagatagctg agcgtctgat gacgcttttc ctgctgccgt
      361 tttgcgctgg cgggccacct ttatctgctg caacccttta tcgtccatca gccgtaacgc
      421 ctgtgttggg cagacgtcaa tacaggcttg cgtgccggaa ctgcgctggt tacaaaggtc
      481 gcatttctgc gcaatcgtat cgaccatctc aacgacgcca aaggggcaag cgattgcgca
      541 tcttttacaa ccaatacatt tttgttcgtt cagttgtacg ctatcagcct gaaaagtcag
      601 agcataaacc ggacaagccg taacgcaggg ggcattgttg caatgatggc aggccaccgg
      661 attcgcagcc tggcccttcc caacaatgtg gatacggggt cgaaagtcac tgtgactcag
      721 cggccagttt tcttgattat gtgccaccgc acaggcaatt tcacaagcat ggcagcctat
      781 acattccgca gcttcagcag cgataaactt attcatttgc atccctttca tttaatgagt
      841 tatgtcttct caaatttcgc gatgcaataa acgggcatat attaggtagc atgaccgttt
      901 taattataat tttgcaactg agttcaaatt attgcccctc aatctatgcc gctttcatta
      961 tttcaacatt gccgatcaaa agaatgctat ccagataaaa acgattaaat gcgagagtgc
     1021 gacatgccag aatgattaat gaaatatcaa tatgagaaaa ttacagataa ttccattgtc
     1081 aggttctctt ctcataatcg tggtttaacc acgattatgt gattgcctcc gcaattcctg
     1141 tctctaactc cccttcctcg caaaaactgg cactccacga gcatgtgttt agacagtttc
     1201 atgaacataa acggttgctt tttactctgg cgggcgaaag gagaaacact gatgagcgcc
     1261 atagattccc aacttccctc atcttctggg caagaccgcc caactgatga ggttgaccgc
     1321 atattatcac caggaaagct gatcatactc ggtctgcaac acgtccttgt catgtacgca
     1381 ggtgcagtcg ctgttcctct tatgattggt gaccgactcg gcctatcaaa agaagctatt
     1441 gcgatcctca ttagctcgga tctcttttgc tgcgggatcg tcacattatt gcaatgtatc
     1501 ggtatcggcc gctttatggg gatccgcctg ccggtgatta tgtcggtgac cttcgccgct
     1561 gtaacaccaa tgatagccat tggtatgaac ccggatatcg gcctgctggg gatattcggt
     1621 gccactatcg ccgcgggttt tatcaccaca ttattagcgc cacttatcgg tcgcttgatg
     1681 cctttattcc cgccactggt taccggtgtg gttattactt ctatcgggct tagcatcatt
     1741 caggtgggta ttgactgggc cgccggaggt aaagggaatc cgcaatatgg taatcccgtt
     1801 tatttaggta tctcctttgc cgtcttaatt tttatcttgc tcattactcg ctatgcgaaa
     1861 ggatttatgt ccaacgttgc cgtattactg gggattgtat ttggcttttt actttcgtgg
     1921 atgatgaatg aagtcaattt atccgggcta catgatgctt catggtttgc gattgtcacg
     1981 ccgatgtcat ttggtatgcc gattttcgat cccgtttcta ttctgaccat gactgccgtg
     2041 ttaatcatcg tgtttatcga gtcgatgggg atgttcctgg cactgggtga aatagtcggt
     2101 cgcaaactct cttcacacga tattattcgc gggctgcgtg tcgatggtgt agggacaatg
     2161 ataggcggaa cgtttaacag cttcccccac acgtcatttt ctcaaaacgt tggcctggtt
     2221 agcgtgacgc gcgttcatag ccgctgggtg tgcattgctt ccggtatcat tttgatcctg
     2281 ttcggcatgg tgcccaaaat ggcggtgctg gtggcctcca ttccgcaatt tgtgctgggc
     2341 ggcgcggggc tggtgatgtt cggcatggta ctggcgacag ggattcgaat tctgtcgcgc
     2401 tgtaactaca ccactaaccg ttacaacctc tatattgtgg cgatcagtct cggtgttggc
     2461 atgacgccaa cgctctctca cgatttcttt tctaagttac cggccgtact gcaaccgttg
     2521 ctgcatagcg gcattatgct cgcaaccctt agcgccgttg tgctgaacgt cttctttaat
     2581 ggctaccagc atcatgctga cctggtgaag gaatccgtct ctgataaaga tttaaaagtc
     2641 aggacagtac gtatgtggct tctgatgcgc aagctgaaga aaaatgagca tggagaataa
     2701 tatgaatctt ttaatgcgcg ctatattcag tctgctgttg ctttttactc tctctattcc
     2761 tgtcatttct gattgtgttg caatggccat tgaaagtcgc ttcaaatata tgatgctgct
     2821 tttttaaatg gtttttacct gtcgacatcc gctcaaaacg ggcggttgtc gataaacgct
     2881 cacttggtta atcatttcac tcttctatta tctataatga tgagtgatca gaattacatg
     2941 tgagaaatta tgcaaacgga acacgtcatt ttattgaatg cacagggagt tcccacgggt
     3001 acgctggaaa agtatgccgc acacacggca gacaccctct tacatctcgc gttttccagt
     3061 tggctgttta atgccaaagg gcaattatta gttacccgcc gcgcccttag caaaaaagca
     3121 tggcctggcg tgtggactaa ctcggtttgt gggcacccac aactgggaga aagcaacgaa
     3181 gaggcggtga tccgccgttg ccgttatgag cttggcgtag aaattacgcc tcctgaatct
     3241 atctatcctg actttcgcta ccgcgccacc gatccgaatg gcattgtgga aaatgaagtg
     3301 tgtccggtat ttgccgcacg cacgaccagt acgttacaga tcaacgatga tgaagtgatg
     3361 gattatcaat ggtgtgattt agcagcggtt ttacgcggta ttgatgctac gccgtgggcg
     3421 ttcagtccgt ggatggtgat gcaggcgaca aatcgcgaag ccagaaaacg attatctgca
     3481 tttacccagc ttaaataaaa aaccccgacg gttgccgggg ttgtgagcat aacgtaatgc
     3541 ttattttacc ggacgcatcg ccgggaacag aataacgtcg cggatggtat ggctgttggt
     3601 gaacagcatt accatacggt cgataccaat tcccagacct gctgtcggcg gtaagccatg
     3661 ttccagtgcg gtgacataat cttcgtcgta gaacatcgct tcgtcgtcac ctgcatcttt
     3721 cgcggcaacc tgatccagga agcgctgcgc ctgatcttcc gcatcattca gctcgctaaa
     3781 gccgttaccg atttcacgcc ctccaatgaa gaactcaaag cggtctgtga tttccgggtt
     3841 aacgtcgtta cgacgcgcca gaggagaaac ttctgccgga tattcagtaa tgaaggtcgg
     3901 ctggatcaga tgagcttctg ccacttcttc gaagatctcg gtaacgatac ggcccagacc
     3961 ccaactcttc tcaacgtgga tgccgataga ttcagcaatc gctttcgcag agtcgaagtt
     4021 gtccagatcc gccatgtcag tttccgggcg atatttcttg atcgcttcac gcatggtcag
     4081 tttttcgaac ggtttaccga agtccagcgt tacgtcgccg taggtcactt ccgtcttacc
     4141 gagaatatcc tgtgccagag tacggaacag cgattcggtc agctcgatca gatctttgta
     4201 atctgcgtaa gccatataga gttccatcat ggtgaactct gggttatgac gtacggaaat
     4261 accttcgtta cggaagttac ggttgatttc gaatacacgc tcgaagccac cgaccaccag
     4321 annnnnnnnn ncttgaggta cagttccggc gcgatacgca ggtacatgtc gagatccagc
     4381 gcgttatggt gggtgatgaa cggacgcgca gcggcaccgc cagggatcac ctgcatcatc
     4441 ggcgtttcaa cttccataaa gccgcggttc accatgaact ggcgaatacc agagaggatc
     4501 tgcgagcgca ctttaaaggt gttacgggat tcatcgttag agatgagatc caaataacgc
     4561 tgacgatagc gcgcttcctg atcctgcaag ccgtggaatt tatccggcag cggacgcagt
     4621 gctttggtca gtagacgcag ctcggtacag tggatagaca gttcgccggt tttggttttg
     4681 aacagtttac ctttcgcgcc gaggatatcg ccgaggtccc attttttgaa ctgctcgttg
     4741 taaacgcctt ccggcagatc gtcacgggca acgtacaact gaatgcgacc gccaacgtcc
     4801 tgcagggtaa cgaaagacgc tttacccata atacgacggg tcatcatgcg gccagcaacg
     4861 gcgacttcga tgttcagcgc ttccagttct tcgttctctt taccgtcgaa ttctgcgtgc
     4921 aattggtcag aggtatgatc gcgacggaaa tcgttcggga aggcaatccc ctgctcacgc
     4981 aggttcgcca gcttctcacg acgcgttttc agttcattgt taagatcgac taccgcgtca
     5041 gcgccctgtg cgtgttgttc agacatgttg gttcctcata accctgcttt caaacttgct
     5101 tcgataaatt gatccaggct gccgtccagc acggcctgcg tgttgcgggt ttctaccccg
     5161 gtacgcagat ctttaatgcg ggagtcatca aggacataag aacgaatctg gctgccccag
     5221 ccgatgtcgg atttgttatc ttccatcgcc tgtttctcgg catttttctt ctgcatctcc
     5281 agttcataaa gcttcgcttt catctgcttc atggcctggt ctttgttctt gtgctgggaa
     5341 cggtcgttct ggcattgggt cacgatcccg gttgggatgt gagtaatacg caccgcagat
     5401 tcggtacggt taacgtgctg accgcccgcg ccggatgcgc gataaacgtc gatgcgcaga
     5461 tccgccggat tgatttcgat atcgnnnnnn nnnncggatt taagccccgc cacttcacct
     5521 tccgactctt cgatgatttc agttttgaaa ccacgcgatt cagcccagcg cagatacata
     5581 cgctcaagca tgctcgccca gtcctgtgct tccgtaccgc cagaccccgc ctgaatatcg
     5641 aggtagcagt cggcgctgtc atattcgcca gagaacatac ggcggaactc aagctgcgcc
     5701 agtttttctt ccagggcgtc gagttcagca acggcttcgt taaaggtttc ttcgtcgtca
     5761 gcttctacag ccagttccag cagaccagaa acatcttcca gcccctgttt catttggtcg
     5821 agggtgtcga caacggcttc gagggaggaa cgctctttac ccagcgcctg tgcgcgttcg
     5881 ggttcgttcc agacatccgg ctgttccagc tcggcgttta cttcttccag acgctctttc
     5941 ttggcgtcat agtcaaagat accccctaag aacgtcggag cgttccgtga ggtcctgaat
     6001 gcgattattt accggattaa tttcaaacat ggtctgattt cttttattga gctagtcaaa
     6061 atgcggtgat aagagcggga ttgtacccaa tccacgctct tttttataga gaagatgacg
     6121 ctaaattggc cagatattgt cgatgataat ttgcaggctg cggttgccgc gaaactcgtt
     6181 gatatcgagc ttataagcca gttgcacttc gcgcacaccg ttatccggcc agagggcggt
     6241 atcgacatta aaagcaatcc cgtccagcag cggaccgccg ccgaccggtt cgaccatgac
     6301 tttcaaatga cgttcgccca ccagccgctg ttgcagcaga cgaaaatgac catcaaacag
     6361 cggctccggg aacatctgcc cccacgggcc agcatcgcgc aacagctgcg ccacttccat
     6421 ggtcatttcg gccggactta acggaccgtc cgacaccact tcgccttgca atagcgaagg
     6481 gtccagccac tccgtaacca gttcgccaaa ccgttgttga aagagttcga atttatcctc
     6541 ttccagcgac aaacccgccg ccatcgcatg accgccaaac ttgagcatca tgccggggta
     6601 gagtgtgtct aatcgctcca gcgcatcacg catatgcagc ccctgaatgg agcgaccgga
     6661 acctttcagc gtaccatcac cagctggcgc aaaggcgata accggacggt gaaaacgctc
     6721 tttgatacgc gatgccagaa tgccaacaac gccctgatgc cattcggggt gatacattgc
     6781 cagcccgccg ggtagcgtgt cgcggctgcg ctccagtttc tcgcacaagg tcagggcttc
     6841 aacttgcatt ccctgttcga tctcttttcg cgtctggttt agcgcatcga gttcatttgc
     6901 cagcacgcgc gcttcgccga tgttgtcgca cagcaacagc gccacgccaa cggacatatc
     6961 gtccagtcgc ccggcnnnnn nnnnncgccc ggcagcattg agacgtggcc ccagcgcaaa
     7021 acctaaatcg ctggcggcga gtttttgtgg atcgcgattg gccacttcca gcagcgcttt
     7081 aatccccgga cggcactttc cggcacggat gcgactcatc ccctgccagg tcagaatgcg
     7141 attattagcg tccagcggca cgacgtccgc cactgtcccc aacgctacca gatccagcag
     7201 ttctgccaga ttaggaattg cgatgccgcg ttcatcaaac cagccctgat cgcgcaaaaa
     7261 agtgcgtaac gccagcatca gataaaacgc cacgccaacg cccgccagtg atttcgacgg
     7321 gaaattacag tcgcgcaagt tagggttaat gatcgcttcc gctgcgggta atgtgtcgcc
     7381 tggcaaatgg tgatcggtaa caataaccgg aatgcccaac gaacgagcgt gttcaacccc
     7441 cgcatgggag gaaataccgt tatccaccgt gacaattaac tgcgcgccac gggcatgagc
     7501 ctgatcgact acttccggac ttaagccgta accgtcttcg aaacggtttg gaaccaggta
     7561 gtcgatattg ctgcaaccaa gcgagcgcat cgccagcacg cttagagccg tgctggtcgc
     7621 accgtcggcg tcgaaatcac cgaccacaat aatccgcgtt ccttcgcgaa aagcgttgta
     7681 aaggatctca acggcctttt cgacgccgct cagttgctgc cagggcagca tg
//
LOCUS       DAWDRC010000119         7691 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4575_length_7691_cov_2.630304, whole genome shotgun sequence.
ACCESSION   DAWDRC010000119 DAWDRC010000000
VERSION     DAWDRC010000119.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4575_length_7691_cov_2.630304"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3108..3117
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4010..4019
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5820..5829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1838 a         1784 c         1982 g         2057 t
ORIGIN      
        1 ccgcgattgg ttgtatctgc ctgatggcga tagtggcggt gatggaagaa cgcaaaatcc
       61 gccgcgagaa aaaaattcag cagttgacag tggcataaac gtaactggtg acttttgccc
      121 ggcatgacgc cgggcttttt tttactattc cgtgaattcc agcgtagtga aggcaaactt
      181 ctcgccatca aacagcccct gactggttag tttcagcgcg gggatcactg gcagggaaag
      241 aaacgccatc tgaataaacg gctcatcggg taacggaccg cattcacggg cggcggcttt
      301 cagggcgtca atttgctccg ccagtgactg cgccgtgtcg gtgctcatta gcccggcaat
      361 gggcaacggt agatgacttt gtacctggcc gttacggacc acgcacagcc cgccgccatc
      421 ctgaatcacc tgattgaccg ccagcgccat ctcttctgcg ctacgaccga tcactacaat
      481 attatggctg tcatggctga ccgtcgcagc cagcgcacct tcattcaggc caaagccgcc
      541 gagcaaacca caggccggag ccagtcgttg tccgtaacgc tcaagaacgg caataaagca
      601 cacatcatcg cgatcaaaac cgttttcgct gtagacgctg gagcgggagt gcgtgatcag
      661 ttcgttatgg attacctcaa taacgcgata gcgttttccg ggggtaaatt gcagggcaaa
      721 gtcgctggcg gaaactggct ggcgatcaat ggtattgcca tatggcgggg cggattgtgc
      781 cagtctcgcc gactcttccg cctgtaaggt ttgtgcatcg atcggctcgc ctttcaccag
      841 tacctgctgc accgtgacct tacgcgcatc gctcaacagg acgatatcgg cctgcttgcc
      901 gggtgccagt aagccgaggt gattcagacc aaaatggcgc gccgtcgacc agctggctac
      961 gcgatatgcc acatgcagcg gtacattgtg ttgttcgatc aggcggcgaa ttaaggcatc
     1021 gatgtgtcct tcatgggcga tctcccacgg gttacggtca tcggtacaga gcatgcattg
     1081 cgggctgtta aattcgttga tcaacggtgc cagcgcgttg agattgcggg ctgcggaccc
     1141 ttcacggatc atcaatgaca tgccgagttg caatttccgg cgtccttctt ccaactgata
     1201 actttcgtgg cagttttcaa tacccgcagc aatataggcg ttaagttctt taccacccaa
     1261 acccgggcag tgaccgtcca gtgtcaggtg gcgaaatgcg tccagtttat cgagcagcgc
     1321 attctgcccg ctaattacgc cagggtagtc catcatttct gcaaggccgg taacctgcgg
     1381 atggtcccgc caggcgagca tctgttcaag ggtaaaactg gcaccattaa catcgcagcc
     1441 ttccagggcg ggtacgcaag agctgacctg taagtactgg ttttgccttg cctgttcggc
     1501 acagcgggca aaccaggcga atccggcttc tcccatcacg ttgacgattt catgagggtc
     1561 gcaaataacg gtcgtcaggc cgcgcggcag ggtagcagtt tcaaaggtga ccggcgtcat
     1621 catgctggat tcaatatgca ggtgagcatc aataaaccct ggcaccgccg ttgcgccgcg
     1681 ggcatcaatc cgctgcaaag ccggagcatc agcgtattct gcgccaacac cggcaatgta
     1741 acgtccttta atcacaattg ggccggaaat ttctccgcca ttgatcaggt cgagaataga
     1801 gacattatca ataatataat cggcaacagc gtctccacgg gaaacggcta acaattcctg
     1861 gtattcagcc cggctaatgt gatgaaattt atggttaata gaattattca tatttttctc
     1921 attttgattt tatcaatttg attattattt atcataaaga aacgtcgcga gattattaaa
     1981 gaatatccat taatgtgcaa ttgaaatgtg ataattatca ctgaaccgtt gttgtcattt
     2041 gttttacaaa agcaagggat taaatctcct ccaatggaca aaaaaatgaa taatgacaat
     2101 accgattacg tgagtaatga atcagggacg ctttcgcgat tatttaaact acctcagcat
     2161 gggaccaccg tccgcacaga attgattgcg gggatgacca cttttttaac catggtgtac
     2221 atcgtttttg tgaacccgca aatcctcggc gcggcacaaa tggacccgaa agtggtgttt
     2281 gttaccacct gtttgattgc cggtatcggc agtattgcga tgggggtatt tgctaactta
     2341 cccgtggcac tggctccggc aatgggcctg aacgccttct ttgccttcgt ggtcgtgggg
     2401 gcgatgggta tctcctggca gaccgggatg ggcgcgatat tctggggcgc agttgggcta
     2461 tttttgctca cgctgtttcg tatccggtac tggatgatct ccaacattcc attaagttta
     2521 cgtattggta tcaccagcgg aatcggatta tttatcgcct taatgggatt aaaaaatact
     2581 ggcgttattg tcgccaataa agacaccctg gtgatgattg gtgatttaag ttctcacggc
     2641 gtgttgttag gtattttagg gttttttatt ataactgtgt tgtcatcacg tcattttcat
     2701 gccgcggtgc ttgtttctat tgtggtgacg tcttgctgtg gattattttt cggtgatgtt
     2761 cattttagcg gcgtctattc cattccgcct gatattagcg gcgtcattgg tgaagtggat
     2821 ttgagcggtg cgttatcact tgaactcgcc ggtatcattt tctcctttat gctgatcaac
     2881 ttatttgatt catcagggac attaattggt gtgactgata aagctggctt aatagatagt
     2941 aacggtaaat tccccaatat gaataaggcg ctgtatgttg atagcgtcag ttcggtggcg
     3001 ggtgcgttta tcggtacctc gtctgttacc gcctatattg aaagtacttc aggtgtggct
     3061 gtcggtggtc gcacggggct gacggctgtc gtagtcggcg ttatgttnnn nnnnnnnccg
     3121 ggtgggcgtt aactgggatg attttaccga atcggtgcct gcgtttatta ccacggtgat
     3181 gatgcccttt acgttctcga tcaccgaagg gattgcacta ggctttatgt cgtactgcat
     3241 catgaaagtg tgcaccgggc gctggcgcga tctgaacctg tgtgtggtgg tggtcgcaac
     3301 tctgtttgca ctgaagatta ttctggtgga ttagtaactg gcgagggttt ccagatatca
     3361 tgagttctga ttacgcagga gaactcatga tctggataat gctcgccacg ctggcggtag
     3421 tgtttgtggt tggttttcgg gtgctgacat ccggggccag aaaagcgctt cgccgtctca
     3481 gcgatcggct gaacatcgat gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt
     3541 cagccggtga cgaattttta cgttatttgc atcgcccgga tgagtcgcac ctgcaaaacg
     3601 ccgcgcaggt gttgctcatc tggcaaattg tcattgtcga tggtagcgaa cagaacctgc
     3661 tgcaatggca tcggatttta caaaaagccc gccttgccgc gccgattact gatgctcagg
     3721 tcaggctggc gctgggtttt ctgcgcgaaa ccgaacctga aatgcaggat atcaatgctt
     3781 ttcagatgcg ctataacgcg ttctttcagc ctgccgaggg cgttcactgg ctgcattaaa
     3841 accgtttcgt cttatcagcc tacagaccgc gcctgacctg gtgggtggat aaggcgttca
     3901 caccgcatcc ggtaatcttt gccctgttcc gtttcgatct taactccagc tcaagcaaca
     3961 tcgtaaaagt tgcaaaaacg ttaaatgcat cacacatttc aatgttagan nnnnnnnnnt
     4021 cggcttttcc cctgaaacat gccacgggta acaccatgag tgaatttatt gctgaaaacc
     4081 gcggcgcgaa tgccatcacc cgaccgaact ggtcagccgt tttctcggtg gcgttttgcg
     4141 tcgcctgtct gattatcgtt gagtttttgc ctgtcagttt gttgacgcca atggcccagg
     4201 atttaggcat ttcggaaggg gttgccgggc aatcggtgac cgtgaccgcc tttgtggcga
     4261 tgtttgccag tttgtttatt acccagacca ttcaggccac tgaccgccgc tacgttgtca
     4321 ttctgttcgc cgttttgctg acgctttcct gtttgctggt ttcctttgcc aactcattca
     4381 gtctgctgtt aatcggtcgc gcctgtctgg ggctggcgct gggcgggttc tgggcgatgt
     4441 cggcgtcgct gaccatgcgt ctggtaccgc cgcgtacggt gccgaaggcg ctgtcggtga
     4501 tcttcggcgc ggtttctatt gcgctggtgg ttgctgcgcc gttgggcagt tttttaggcg
     4561 agcttatcgg ttggcgcaat gtctttaatg cggcggcggc gatgggcgtg ctgtgtattt
     4621 tctggattat caaatcactg ccttcgctgc caggtgaatc ttcgcatcag aaacaaaata
     4681 ctttccgctt attacaacgt ccgggagtga tggccgggat gatcgccatc tttatgtcct
     4741 ttgccggaca gtttgctttt ttcacttata ttcgcccagt gtatatgaat ctggcgggat
     4801 tcggtgttga tggcttaacc ctggtgctgc tgagttttgg tatcgccagc tttgtcggga
     4861 catcgctttc gtcgtttatt ctcaaacgtt cggtgaaact ggccttagcg ggcgcaccgt
     4921 ttgtgctcgc cctgagcgcg ctggtactga cgctgtgggg aagcgataaa attgttgcta
     4981 ctggtgtggc gattatctgg gggctgacct ttgctttaat tcccgttggt tggtcaacgt
     5041 ggatcactcg ctcattggcc gatcaggcag aaaaggctgg atctattcag gtggccgtta
     5101 ttcagcttgc taacacctgc ggtgcggcaa tcggcggtta tgtgttggat aacattggtc
     5161 tgacttcgcc gctgatgttg tccggcacat tgatgttgct gactgcattg ttggtgactg
     5221 caaaggtgaa aatgaagaaa tcctgatatc aggtaaagaa gcgttggtca gtggttgttg
     5281 ccgttcttac atatccgggc attctacctt acccaacgct tcccaataat tgctctgatt
     5341 attacgttcc atcgccacga tggcctcgcg tatatgttgt ttattgtttt catctgacaa
     5401 cattaacttt tcccacgctt catctgataa tgacgcctgt ggcgtgcaaa ttttacgtaa
     5461 atcttgcaat accgtggttt tctcttttgc cgactgtaga gaactgaatg gcgactcggc
     5521 ctgagaaatg gctggaaaaa tcaggaatac ggtaatcatg agtagcatcg ttggcttcat
     5581 agcggcaacc ttctgcaaat gtgcatcgaa tgaagcataa gtgtagtttg cttttgccgc
     5641 tctcatcatg ctgtacgaaa ggtgtttgcg tggtcagtaa gaagtgccag actttatatt
     5701 ccacttttat tcctttttat tcttatcgat agcgtttcgt tttttaaact gcagcgacct
     5761 taccgctata gtcaggtaat cattaataaa aggataaaaa aatgaaactg acaacacatn
     5821 nnnnnnnnng ttgcgaccag agcagtagcg atgaaaagca tattaaagtt ggcgttatta
     5881 atggcgcaga acaagatgtc gcggaagtcg ctaaaaaggt agcaaaagag aaatatggtc
     5941 tcgatgttga actggtagga tttagcggtt cgttgctacc gaacgatgcg acaaatcatg
     6001 gcgagcttga tgcgaacgtc ttccagcatc gaccctttct tgaacaagat aatcaggcgc
     6061 atggttataa actggtagcg gtgggaaata cgtttgtctt ccccatggcg ggctattcca
     6121 aaaaaatcaa aacagtggcg caaataaaag aaggtgcgac agtggcgatc cccaacgacc
     6181 cgactaacct tggtcgcgcg cttttactgc tgcaaaaaga gaagctaatt actctgaaag
     6241 aaggaaaagg gttattacct accgcgttgg atataaccga taatccacgc catttgcaga
     6301 taatggagct ggaaggggca cagttgccac gtgtactgga cgacccgaaa gtggatgtag
     6361 cgattatcag caccacttac atccagcaaa caggactttc tccggttaac gacagcgtat
     6421 ttattgaaga taaaaattcg ccgtatgtga atattctggt ggcacgggaa gataataaga
     6481 atgcagaaaa cgtgaaggaa tttctgcaat cttatcaatc acccgaagtc gctaaagcgg
     6541 cagaaaccat ctttaacggt ggcgcggtgc ctggctggta attctcactc atgccgcgcc
     6601 ggacgattta tttttctggc gcggcggcgg gaatccattg aaatcaaaat caccgatgac
     6661 agaatcagca gcgttcccag ccagtccggc aatgtgaagg tgatccccag caacaacaaa
     6721 gagagcagcg cgctacttaa tggttctgca cagcttaaaa tgctggcttt tggaccgcca
     6781 attaattgtg ctcctttcag gtacagacta aatgtcaggg acgtaccaat gaccaccaaa
     6841 taaaaaaacg ccagaatcaa actgccgtta acgacaaagt ttgtcccttg tctggcataa
     6901 aagggcagca ggagcagacc gccaatgagc atactccagc cgacgaccgg taacgtgcca
     6961 tagcgggcaa tcagcgttga gggataggtg gtatagaatg cagcagcaaa tgccgaggca
     7021 atcccccaga ataacgcggc aggagagatc gataatgacg tcggattacc gtgtgtcacc
     7081 aataaaaaag tcccgatcag cgatgtcaaa atagcgcaaa aaaccaggat gccggggcgg
     7141 gatttacgca ccagtgagaa ccaggcgacg ataatagtcg gtgagaggaa ttgcagcacc
     7201 gttgccgtgg ctgcgttcga tttttcgatg gttagcaaaa aagtgagctg tacggttagc
     7261 gcgccaacca cggaaaaaat cagtaggcta atggcatctt tgtgattgtt aataatagaa
     7321 aagattttat caccatgaac aaatgatagc gtcagcagaa tcaaaccggc gaatatcaaa
     7381 cgcgtcatag tcaaaaactg cgacgacatc tggctttgct ccatgatgta ttgcgcgcag
     7441 acccctgaac ttccccacaa cacggcggca atcagaacgt tcagcatccc ctttctggtg
     7501 gaacccattt ttccctctgc atactgtttt gtcttttcat caatatacgt taaggatacc
     7561 actaccgatg acggtgaggg agtagagtaa tcaatcagtt ttagtgaatg gaggaaataa
     7621 tgacatataa caattattca tatggtgttt aaaaaaactc aatatgaaat ttaattattt
     7681 aatgttcaat a
//
LOCUS       DAWDRC010000120         7687 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4580_length_7687_cov_2.683831, whole genome shotgun sequence.
ACCESSION   DAWDRC010000120 DAWDRC010000000
VERSION     DAWDRC010000120.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7687)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7687)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4580_length_7687_cov_2.683831"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7312..7321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1764 a         1887 c         2063 g         1963 t
ORIGIN      
        1 ctggcgtcat gccgatctgg cggacctcga acatattcgc catgcagccg atcagttgtg
       61 ggaagattcg ctgttcgccc agccaattca ggcgttgttt catgccgctt acgctaacgc
      121 ctcgttgtat gctctgcgat ctgccgcgca taaactgttg aattacgcgc agcaggcgcg
      181 ggaatacctg gattttcgtg cgcacgggtt aaacgtcgct tgtgaacaat tgcggcaaaa
      241 tatccaccag atcgaagaaa gtttgcagct atttcaactc aatcaggcgc aggtgagcgg
      301 cgagattaaa catgaaatcg agctggccct gacctccgcc aaccactttc tgcgtcaaca
      361 gcaagatgcg gtgaatgccc agttagccgc gttgtttcag gatgattcgg ggtcattaag
      421 cgagattcgt acctgctgtg agacactgtt acagacggcg cagaacacca tcagtcgcga
      481 ctttacgctg cgttttgccg agctggaatc taccctttgc cgggtgttaa ccgatgttat
      541 tcggcccatt gagcaacaag tcaaaaggga attgagcgag tctgggtttc gtcctgggtt
      601 tcattttcct gtttttcacg gcgcagctcc ccacttcaac actcgccagt tgttcagtga
      661 agtcatctcc cgccatgcag caacggacga gcagagtacg cgtttaggcg ttgtgcgtga
      721 gactttttcg cgctggttga atcagcccga ctggggacgg ggaaatgaga aatccccgac
      781 agagacggtt gattacagcg tgttgcagcg agcattaagc gcagaagtcg atctttattg
      841 ccaacaaatg gctaaagttc tggcagagca ggtcgatgaa tctgttacgg caggtatgaa
      901 tacttttttc gctgagttcg cttcatgttt gacggaatta cagacgcgtt tacgcgaaag
      961 tatggctctg cgtcaacaaa atgaatcggt ggtcaggctg atgcaacagc aattgcagca
     1021 gactgtgatg actcacggct ggatttacac cgacgctcag ctgttaagcg atgatattca
     1081 aacacttttc acggcagaac gatattgacc aagacgttac tggacggccc cggtcgcgtg
     1141 ctggagtcgg ttcatccgcg ctttttagtg gatctggcgc agggcgatga tgcccgcctt
     1201 ccacaagccc atcagcagca gtttcgtgaa cgactgatgc aggaacttct tgcgcgcgtg
     1261 cagcttcaga catggacgaa cggcggcatg ttaaatgcgc cgcttagtct gcgtctgaca
     1321 ttggaggaga aactggcgtc gatgctggat cccggtcatc tggcgctgac gcagatcgcg
     1381 cagcatctgg cgctgctgca aaaaatggat caccgccagc actctgcttt cccggagctc
     1441 ccccagcaaa ttgtcgcctt gtatgagtgg ttttcagccc gttgtcgctg gaaggaaaag
     1501 gcgttaacgc aacgaggcct actggtgcag gcgggtgaac agagcgagca aatttttacc
     1561 cgctggcgtt ctggggcgta taacgcctgg tcgttgcctg ggcgctgctt tatcgttctg
     1621 gaggagttgc gctggggggc atttggcgat gcctgccgtc tgggaagccc gcaagcggtg
     1681 gcgttgttgc tgggtgattt gcgcgtgaaa gcgacacaac atttggcaga gagtatcaat
     1741 gcggccccga ccacgcgtca ctattaccat cagtggtttg cctcttcgac cgtttcgacg
     1801 ggcggggagc atgctgattt tttaagttgg ctgggaaagt ggtccacggc agataaacaa
     1861 cccgtttgct ggtcagtgac ccaacgctgg cgaactgtcg cactggggat gccacgactc
     1921 tgttcagcgc agcgtctggc gggggcaatg gtcgaggaaa tcttctctgt aaatttggtg
     1981 taaataatca gttacaccaa tgagtcctaa acgaaataca tgtgtgaagt tgatcacaaa
     2041 tttaaacact ggtagggtaa aaaggtcatt aactgcccaa ttcaggcgtc aactggtttg
     2101 attgtacatt ccttaaccgg agggtgtaag caaacccgct acgcttgtta cagagattgc
     2161 atcctgcaat tcccgctccc cttttgcggc cgtcgcgctg atttttctgg cgtttgcgga
     2221 aatgggccaa ctctgcgagg aaagctatgc tgaaaaggaa aaaagtaaaa ccgattaccc
     2281 ttcgtgatgt caccattatt gatgatggta aactgcgtaa agccattacc gcagcatcac
     2341 tgggtaatgc aatggaatgg ttcgattttg gtgtttatgg ttttgttgct tacgcattag
     2401 gtaaagtttt tttcccgggg gctgacccca gcgtgcagat ggttgctgca ctcgccactt
     2461 tctccgttcc ctttctgatt cgaccgcttg gcgggctctt ctttggtatg ttgggcgata
     2521 aatatggtcg ccagaagatc ctcgctatca ctattgtgat tatgtcgatc agtacgttct
     2581 gtattggctt aataccgtcc tacgacacga ttggtatttg ggcaccgatt ctgctgttga
     2641 tctgtaagat ggcacaaggt ttctcggtcg gcggtgaata taccggggcg tcaatatttg
     2701 ttgcggaata ctcccctgac cgtaaacgtg gctttatggg cagctggctg gactttggtt
     2761 ctattgccgg gtttgtgctg ggtgctggcg tagtggtgtt aatttcgacc attgtcggcg
     2821 aagagaactt cctcgattgg ggctggcgta ttccgttctt tatcgctctg ccgttaggga
     2881 ttatcgggct ttacctgcgc catgcgctgg aagagactcc ggcgttccag cagcatgtcg
     2941 ataaactgga acagggcgac cgcgaaggtt tgcaggatgg cccgaaagtc tcgtttaaag
     3001 agattgccac caaacactgg cgcagcctgt tgacatgtat tggtctggta attgccacca
     3061 acgtgactta ctacatgttg ctgacctata tgccgagtta tttgtcgcat aacctgcatt
     3121 actccgaaga ccacggggtg ctgattatta tcgccattat gatcggtatg ctgtttgttc
     3181 aaccggtgat gggcttgctg agtgaccgtt ttggccgtcg tccgtttgtg cttcttggta
     3241 gtgttgcact gtttgtgttg gcgatcccgg cgtttattct gattaacagt aacgtcatcg
     3301 gcctgatttt tgccggctta ttgatgctgg cggtgatcct taactgcttt acgggcgtta
     3361 tggcttctac cttgcctgcg atgttcccga cgcatatccg ttacagcgcg ctggcggcgg
     3421 catttaatat ttcggtgctg gttgccggtc tgacgccaac gctggcggcc tggctggtcg
     3481 aaagctcgca gaatctgatg atgcctgcct attacctgat ggtagtggcg gtggttggtt
     3541 taatcaccgg cgtaaccatg aaagagacgg cgaatcgacc gttgaaaggt gcgacaccag
     3601 cggcgtcaga tatacaggaa gcgaaggaaa ttctcgtcga gcattacgat aatatcgagc
     3661 agaaaatcga tgatattgac cacgagattg ccgatttgca ggcgaaacgt acccgcctgg
     3721 tgcagcaaca tccgcgaatt gatgaataag ctgaaacgga tggcctgatg tgacgctgtc
     3781 ttatcaggtc aattgaactc ttaaggttca cttaatctct gacgcgcata ctctcctcca
     3841 ggttaacgga ggagagtgca atgaaaaacc gtgtttatga aagtttaact accgtgttca
     3901 gcgtgctggt ggtcagcagc tttctttata tctggtttgc cacgtactga tcttttttca
     3961 gccgtatcca ggcccgcgtg ccggaagtct cttgccggtt ttgcaggaaa aactgcccgt
     4021 gatgcaactg tgtaatgcgg ctgacaatac ttaaccccag accaatcccg ccataacggc
     4081 tgtccattcg tacaaacgct ttactcaact ccccgcattt actctcatca atacccggtc
     4141 cttcatcttc aacggccatg accgctccgc catcttcttg cagcttaatc ataatgttgc
     4201 tgccttgcgg gctataacga tgggcgtttt ctaccaggtt tcgcaataac atccgcagca
     4261 gggttgcatc accctgaacg gtgatgtcgg cggcgctctc tggcaatagc agggtttgct
     4321 ggcgctggtc gagcatggta ctgagttcgt catacgaggg gagaatgaca tcttccagca
     4381 gttttacatg ttgataatta ccggaagaaa atgactgtcc ggcacgcgcc agttgcagca
     4441 gctgggagac gctctccatc atctgatcaa gtcgtgccac taacggtgct acatcaatgt
     4501 gatgcgtttt cgccagcagt tccagatgca aacgcacccc cgccagtggc gttcgcagtt
     4561 cgtgcgcgac gtcagcggta aacaaccttt cgttatccag cgtgctggtc aggcgactga
     4621 ccagatcgtt taatgccgaa accaccgcat cgatttcgag ggtggcgcta tgaatggcaa
     4681 tgggcgttaa gttgtcggcg gtgcgcgctt ccagctcttt ttgcagctcc gccagcggtc
     4741 gggtgatgcg gcgtaccgcc tgatagcaga taaatagcgt caggctaacc ataaagacgc
     4801 cggggacaat caggctggcg accgcctcgc ggatctcacg catgatgtgg cgatcgttgt
     4861 tgcgattgtc gcgtagcgcc tgctcaaaca gctgaatctg ctcggtactt tcatgccata
     4921 gccagaagac gctgatcagc tcaaacacca acaaaatggc cccgatggtc aatatcagcc
     4981 gttggcgcag cgatattggt cggcgcagaa aacgcatcag attcaattag ttttcctcat
     5041 ttgcgaccag catatagcca aatccgcgca cggtgcggat acgggctttg cccactttgt
     5101 cgcgcaggtt atggatatgc acttccaggg tgttggtcga gggctcgttg tcccagttgt
     5161 agatatcgtt gtagagaatt tcccgatgca ccgggctgcc tgctttaagc atcaggcgcg
     5221 acagcagagc atattctttg ggcgtcagaa tcagttcttc tccgctcatc catacctggc
     5281 ggcgacccat attcagcgtc aggttgccaa cgttcagctc actttcgccc tgattattat
     5341 ggcgtcgtag cagggcgcgg atacgggcgt gcaactcttc cagcgcaaaa ggcttcacca
     5401 gataatcgtc ggccccgaca tccagcccgg cgattttgtc ggtaagagta tcgcgggcgg
     5461 tgagaattaa caccggcaga gtgtattttt tctgccggat acgggcaaga aaatgcagcc
     5521 catcttcatc gggtaagcct aaatccagta ccaccaggct gtaatgaccg gcctcaaggc
     5581 tttgttccgc catccgcgcg gtgctcacgc catcgcacgc gtagccttcg gtttgcgccg
     5641 ccagaatcaa tccctgcaat aacagcgtat cgtcttcaac aatcagaatt ttcattcact
     5701 cactctcctg caagtttgca gaatatcatc cgcagcctgg taatacttcg tctcaacgcc
     5761 agtcaggccc aataaggttg agaataaatt gtcttgtgaa tagtgttgcg tttgcgcctg
     5821 tttttgcagg cagttctggt caacctgata ccgtttttga taatcctccg atagccacag
     5881 cagcatcggc acctgttttt ggctatccgg ggcgatggca taaggcagac cgtgcagata
     5941 gatgccattt tcacctaacg attcaccgtg gtcagaaaga taaaccaggc tggtggtaaa
     6001 tttatcctga tgttctttca gcagattaat cgctttatca acaatatagt cgacgtaaac
     6061 cagcgtgttg tcgtaagtgt tcaccagttg ctctttggta caggtctgga tctcgttagt
     6121 gtcgcaggtt ggggtaaatt tcctgaactg cggcggatag cggttgtaat aggtcgggcc
     6181 gtggctgccg atagtgtgta atacaatgag gccatcgctt tgcagattat tgatgtactc
     6241 ttccagcccg tggaacagca cttcgtcata gcattcgccg ttgatgcact gaccaggcag
     6301 gttcagcgcg gtgatgttct ggtgaggtac gcgatcgcaa acgcctttac agccgccatc
     6361 gttgtcattc cacagcacgt tgatgcccgc tcgctgaatg atatccagca cgccttcctg
     6421 gtgctgcgcc agctcttctt tgtagtgctc acgcggcata tccgagaaca tgcatggtac
     6481 tgaaactgcc gttgctgtgc cgcaagaggc ggtattaggg aaatagacca cgttatcttt
     6541 tgccagacga ggattggttt cgcgtggata accattcagc gagaaattct ctgcgcgcga
     6601 cgtttctccg acgatcaaaa tggtcaggtt tttacgtttc ccattttgca ttaatgggtt
     6661 gcggtgagca tcttcaccaa tgcgtatcag tggcaggttg gctgaccgct ggtgagagta
     6721 ccacgaccag ctagcaacaa tgctgttgga cgggcttaag gatttcacca gctctttgtt
     6781 gttgcgaaac agcgaggcat agtctttgta gaacagtgcg gcaaccagca gaatcagcag
     6841 cacagaaatc agtatgttgg ccccgcggaa aagaacgctg cgcaggcgtg aggtggtggg
     6901 tttgattttg atccagcagg caataatcgc ggccaacacg ccacttaacc ccagcgttaa
     6961 taacattcgt ggcgtcatca acgcataact ttccgccggc gtagtatcaa tgatattggt
     7021 gatcattgaa cggtcgatga cgatgccata ggtcatgatg aaatattgtg cagccgcacc
     7081 gaccagaata aacaggcagg cgagtggacg attaagccat aagaaagagc ctagtgtcag
     7141 gacaatatta atcacgctga atgcgacgac cggcatcgac aagaaaacca gcacgttatg
     7201 cagtgaatcc agcggtaaca cctgtagcac ctgtttaaaa aaggcgatat tcaggcagac
     7261 tgagatataa aaagtagcaa gtaataacca ggagaataaa ttcaaagagg gnnnnnnnnn
     7321 nttaagggga attaatcttt gcttattggt gcatctaagg gatacgggtt tttatgcagc
     7381 cggttgtaat tcagcgcata catggcggtg attaccatca gagtgacaaa tgaccacata
     7441 acctctttcg cgccggaccc tactacggcc cagatgcagt agaggaaggc aatggtagta
     7501 actgccaggt atgccgggcg tgctttacca aagtgaccgt ggccgagcag tagtaacgcc
     7561 gcacaggtgt aaagatatgg caccagtgta aagatgaccg acacggaaga aaccagaccg
     7621 aattctttgg tcgcgtttgg cgaaatgcta ctgagctgga agatggtcat caaaataccg
     7681 acgataa
//
LOCUS       DAWDRC010000121         7658 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4595_length_7658_cov_2.086413, whole genome shotgun sequence.
ACCESSION   DAWDRC010000121 DAWDRC010000000
VERSION     DAWDRC010000121.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_7658_cov_2.086413"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    875..884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5756..5765
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7350..7359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1946 a         1983 c         1933 g         1766 t
ORIGIN      
        1 atcagccagt tgcaggcgtt tcaccggcag cttatgcagc agcacgtttt ccagttccac
       61 tttgttgaag tgttccgtgc catcgccacc aaagtacggg aagcccacct cagcgatctc
      121 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
      181 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
      241 aaccatttca ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
      301 cagtgcatcc accagatcag cagcgcgcag catacgaccc gcagcgtagt aaccgtcgcg
      361 ctcttccagc atcaccagca tcggcatgtc ggtgtagcgg cgcacatagt cggtgaaata
      421 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tgacccattg ccagcgccat
      481 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
      541 gtagtctggt gtgacggcaa cagttttggt tcctttgtaa cgcacttcag taaagaagtg
      601 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
      661 gtaccagtca gcagattccg gtacgtcagt ttgctcaccc caggtttgcg gagacgcagg
      721 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
      781 gcgcgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga agccagcgac
      841 acggtccggg ccgtaatttt tgatggtgta aacgnnnnnn nnnncgcttt atcggcgtct
      901 tcaatgatag aagcccatgc ctcaaccgga tcgctatgca gcgccttcgc ttcacgccac
      961 attttcatca ggcgtttgcg catcatcggg tatttcaggc ggttagcgct gtatagatac
     1021 caggagtagc tggcaccgcg cgggcagccg cgaggttcat ggtttggcag gtccgggcga
     1081 gttcgcggat agtcagtctg ctgggtttcc caggtgacca gaccgttttt gacgtagatt
     1141 ttccagctgc aggagccggt gcagtttacc ccgtgggtag agcggacgat tttgtcatgc
     1201 tgccaacgct ggcgatatcc atcctcccag tcacggttgg tattgagaag ctggccatgc
     1261 ccatcggcaa aggtttcacc cttctgtttg aagtagcgaa accggtccag gaatttactc
     1321 atcggttttc tcctgtggga gcctgtcggc cctctgataa atcgacattg ctaatgttta
     1381 agggcaaggt aacgctctga aacagcgtgg gaattgataa cgatcaagag tgatggggat
     1441 gaaaaataaa gtaaattcct ggaatacccc tttaggataa ttaacattta tagcataaca
     1501 gactgtaaat taagacgttt tacacctgta gttatttaaa ccattaagat ttttatgcag
     1561 atgggtatta aggagtattc cccatgggta acatattaat gactcttaat gaaatccagc
     1621 gaaataagcc atacaacatt agtatgttgt aactaaatga cgtataaaaa aagcgcgaca
     1681 tcatgccgcg caaaggataa tcaaatatta cgatttattt tttagaatgc cgaccatata
     1741 ccgcccaggt aatcaccacg caggcgatat agaaaatcaa aaatactttc attgcgccga
     1801 ctggcgaacc cgttaatgcc agcgagctac caaacgcttt cgggataaag aagccaccaa
     1861 tcgcgccaat cgcagagatg aaacccagcg ccgctgccgt gtcggttgcc gcttcacgca
     1921 tcgcacgttc gtcagaaccc ccttctgctt tcacgcgatc cattgtcagt ttacggaaga
     1981 tcacggaaat catctggaaa gtggaaccac tacccagccc agcggtcagg aacagcgcca
     2041 ggaagactgc gaagaacgcc atgaagcttc cgccctgtcc gtcagtcggt aaggtcagga
     2101 acagcaggcc gctgaaaatc gccatcagga taaagttcac cagagtgaca cgagttccgc
     2161 ccagacggtc agataatgca ccacctgcag aacgcgccag cgcaccaata aacggcccga
     2221 agaaagcgta ttgcagaatc tgaacatccg ggaactgcgt ttttgacagc atcgcaaagc
     2281 ccgcggagaa gccgatgaag gagccgaagg ttgccagata cagcaggctc ataatccaca
     2341 gatgaccccg tttgagtacc ggcaactgct ccttgatgga ggctttcgag gtagcaagat
     2401 cgttcatgcc aaaccacgcc gcaatggtga agatggcaag gaccggcacc catatccagg
     2461 acgcattcgc cagatacagc tcagtcccat ccggctgttt gacgccctgg ctaccaaata
     2521 ctgcgaaaat cgacagtgat accaccagcg gagcaaccaa ctgcatgacg ctgacgccca
     2581 tgttgcccag accaccattc agacccagcg cgccaccctg cttctgtttc ggaaagaaga
     2641 agctgatgtt tgccatactg gatgcgaagt tcgcgccagc aaagccgcac agcagagaaa
     2701 tgatgatgaa gacgctataa ggcgtggagg tatcctgcac ggcaaaaccc agccagacgc
     2761 aaggaataat cagaataccg gtgctgaacg ccgtccagcg acgaccacca aagataggaa
     2821 ccataaagga gtatggaaca cgtaataacg cgccagaaac cgaaggcagc gcagtcaaca
     2881 taaatagctg atcggtcgta aaattaaaac cgactttcgg taagttcacc gcaacagcgc
     2941 tgaacaacat ccatacgcaa aacgccagca acagacaggg aacggaaatc cacaggttgc
     3001 ggctggcaat acgttgaccg cgttgttgcc agaacgcagg atcttccggt cgccaatctg
     3061 taatgacagc tccagtagcc ctttcggggg cggatgagtg actcatagac acctctgata
     3121 ctcgtttcga tgtctgccac cttagtgtct gtagctaaag gcaatttgat gtaaatcaaa
     3181 cgataagata actttatcta tcattgatat ttatcattat ccatagtgag tacagtgatt
     3241 tcataaaaat tatgagattt ttcacggtac tgtaaaatcc ctacccttac cgatgtaaag
     3301 cgactaacca cacggcaaat aaggagtaac tctttccggg tatgggtata cttcagccaa
     3361 tagccgagaa tactgccatt ccagaatgta tcgtcacatt cattaaggtt attgctcatt
     3421 taaagcctga aggaagaggt ttacatgctt aaacgttgtc tctctccgct caccctggtt
     3481 aatcaggttg cgcttattgt gttgctttct actgctattg gactggcagg gatggcggtt
     3541 tctggctggc tggtgcaagg cgttcagggc agcgcccatg cgatcaacaa agcgggatcg
     3601 ctgcgcatgc aaagttaccg tctgttggca gcagtgccat taagcgagaa agacaagccc
     3661 ttaattaaag agatggaaca aacagcattt agcgccgagt tgactcgagc agcagaacga
     3721 gacgggcaac tggcgcaatt acagggttta caagattact ggcgtaatga actgatccct
     3781 gcactgatac gtgcacaaaa ccgcgaaacg gtgtcagcgg atgtcagcca gtttgttgcc
     3841 gggcttgatc agctggtatc tggttttgat cgcaccacgg aaatgcgcat tgagacagtg
     3901 gtgctggtcc atcgggtaat ggcggtattt atggcacttt tactggtatt cactattatc
     3961 tggttgcggg cgagattgct acaaccgtgg cggcaactgc tggcaatggc gagtgccgtc
     4021 agccatcgcg attttaccca acgcgcaaac atcagcgggc gcaacgaaat ggcgatgctt
     4081 ggaactgcgt tgaacaatat gtctgcagaa ctggccgaaa gttatgccgt acttgagcag
     4141 cgggttcagg agaaaaccgc cgggctggag cataaaaatc agatcctctc ttttttatgg
     4201 caggccaacc gccgattgca ttcccgcgcc ccgctgtgtg aacgcctgtc acctgtactc
     4261 aacggattac agaatttaac cctgctacgt gatatcgaac tgcgggtgta tgacactgat
     4321 gatgaagaga atcatcagga gtttacctgc cagccagata tgacttgtgc cagctctgcc
     4381 cgcgcggcgt attacccgtt ggcgatcgcg gcacaaccct gaagtggcgg ctggctgaca
     4441 ctcatacgca gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg
     4501 atcaacaaca actggtggat accctggttg aacaactcac cgccacgctg gcgctggatc
     4561 gccatcagga acgtcagcaa cagttgatcg tgctggaaga gcgtgccacc attgcgcgcg
     4621 aactgcatga ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgcttac
     4681 agatgcaggg cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg
     4741 aactgaatgc atcctgggtg cagttgcgtg aattgctcac cacattccgt ttgcagctca
     4801 ccgagcctgg attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg
     4861 gcttcccggt gaagctggat tatcaattgc cgcctcgtct ggtgccttcg catcaggcaa
     4921 tccacttgtt gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga
     4981 gtgaggtcgt ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata
     5041 acggctgcgg cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc
     5101 gcgaccgtgc gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca
     5161 ccgaagtggt ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc
     5221 atgagtaatc aggaaccggc tactatcctg ctgatcgacg atcacccgat gttgcgaact
     5281 ggcgtaaaac agcttatcag tatggcacca gatatcactg tggttggcga agcgagtaat
     5341 ggcgaacagg gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc
     5401 aatatgcccg gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctcc
     5461 gggcgcattg tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa
     5521 cggggcgcgg atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcgtta
     5581 catcaggcag ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc
     5641 agcttgcgcg ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag
     5701 cgcgatattc tcaagctgat tgcccaggca atcatcttgt tcggcaaacc ctgggnnnnn
     5761 nnnnnccagg gtttgccgaa caagatgatt gcccgccgcc tggacatcac cgaaagcaca
     5821 gtaaaagttc acgtcaagca catgctgaag aaaatgaagc taaagtctcg cgtggaagct
     5881 gcggtatggg tgcatcagga gcgcattttc tgattacggt tcccagcgca gttcgtcgtt
     5941 tgacaatgcg tcgaacggtt cgacaagcgt tagcgtgatc tcattggagg agacacgctg
     6001 cccctggtta tcttccacca ccactgacaa tcgccagtga ttgcttgcgc cttccccgtt
     6061 ttgccagtca ggcatgatca gcgtccagcc ctcctcactg ttggcttgtg cgcccggcgt
     6121 caaacttaat atctgcgtat cgccctgcca aatcagttgc cgaataccgt aacgactgcg
     6181 gatttgtaat ttcagcggca ctgtttcgcc aggttttaga tcccacggag gtgtcgccag
     6241 aaacaccgtt aacgtttttc gctgtcggta ctcaagagtc ggcagattat ttcgctgcgg
     6301 attgtcatag cgactaccac gtaacgactg actttcggca acctcgcccg cagaaagttg
     6361 ttttttgagc ggtacaccaa agcggtagtt aagattcagc ccgaggttat tttgattctc
     6421 gccactttca ccctgtttat gctgggccgt cacagtgact aagggcacag gggtgtaatt
     6481 taatcccaga ctcaacgcga cgggattgtg ataacccgta ccagagttaa acaaatcgac
     6541 acgatcacca aaatactgtt ctacgctgac actggtattg aggtgttgat agaacggcat
     6601 gcgcatccgg gctgtcaggt cgtacccgcg cgccatccgt tgttcctgcg tggctgtctg
     6661 ttcatgccat gcagcaaacg gctgataaaa gtttgccgat agtcgcaaat attcgcccca
     6721 cgcttcagcg ccaaagcccg ctcgctgaag attttcgtcc agcaagttgt cataaaaagt
     6781 gttataaccc accagccagc tgccgcgcgc ccagcgttgc ccaacgccca cattgctcac
     6841 cagcccatca tcctgctgag taagaccaag ctggctccag gtgagataac gatcattatc
     6901 ttgtaacggc acaaaccagc ttccacgact gccggtgaaa tgtccttcgt tatccacttt
     6961 gacgtcaaca ctggcatttc cccacggtga tagccaggac tctacgtgct gattaacctg
     7021 ttgactaagc gcgtcgcgga cctttcccaa tgcgaaagct tttgcctgct cgccagtatc
     7081 cagcccgtta tcagtcatac tggtttcgca gaaatctttc acaatttcag caaagtgttt
     7141 ttccccatca tgattttcag gtgccatgcc taaatccggc agaccatcat tgttattatc
     7201 aaagggattt gccgcttttt gctcgaaggt agattgtgcg ttagctgtac cgcctgccgc
     7261 cagcaagagt aaataaaacg gaataatgcg aggaacgaaa cggctcaaaa cgtcggtaac
     7321 ttccggtaaa aatgaaaaac catagccttn nnnnnnnnnc cttaaagaat aacgattact
     7381 ggcacctttt gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg tgaccgattg
     7441 gtcagacgct gcagaagcag taagatagaa gatgagtgtc taattccctg aatacaggag
     7501 taaataatgc aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag cgaatccttg
     7561 tttaacagct tgcggctggc cattgcgtta cgagagcagg agaacaatct ggatctgcgt
     7621 ctgttcctga tgtctgatgc ggtcacagcc gggttgcg
//
LOCUS       DAWDRC010000122         7648 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4602_length_7648_cov_2.426841, whole genome shotgun sequence.
ACCESSION   DAWDRC010000122 DAWDRC010000000
VERSION     DAWDRC010000122.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7648)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7648)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4602_length_7648_cov_2.426841"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4774..4783
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2049 a         1791 c         1741 g         2057 t
ORIGIN      
        1 ttctcatagc taccataaac tatactagcc tgaactatat ttattctgct gcaatcaatg
       61 catacataac acaaatatca ctcaggtaca ctactcaaac cacgctggga tttttcctca
      121 agttataatc atcaccccga aaatcattcg gcatttactc attaaatagt caccccatag
      181 gcctgtacat gtttactcag aaatatacat ccttttctct gtcataaacc ctctgattaa
      241 tcataaataa atacttgtga caccaatctt tttccttaac ggaacgaatt gttgtgtaga
      301 aggagataat attatgtcaa aaaaatatca gccattgctt ataactcatt atatgtcaac
      361 atgggtcact ataacggaag cagttgagat cactaccaaa gccataaaac aaaaaattac
      421 tcctagcgat atttatcgtc atgccttgag tggcaacatc ctactatcgg tctatttcca
      481 gtctcctgtg atacttaaga agatacaaac ttttaatgga aaaataaaat tcaggcagtt
      541 tgtgggagac ctacttgata aactatgcat gcttgacagg gacggtttta tttatggtca
      601 aaatttaaga ctctgtacag aagccagata tatttgtcct gtgcaacaaa ttatagatac
      661 ccccctgtta cgcaaactca accagtttcg tacgtttgtt cgaggttttg ataatgtccg
      721 cccgggtgat gaactggatg tcccggcaca agttagtgaa aaaaatttaa ccccgccgcc
      781 gggtaacagc agtggcaacc ttgagcaaca gatagccagt acttcacagc taattggatc
      841 tctgctcgcc gaagatatga acagcgagca agcggcaaac atagcacgtg gatgggcctc
      901 ttctcaggct tcaggggtaa tgacagactg gttaagccgc ttcggtaccg caagaatcac
      961 gctgggcgtg gatgaagatt tcagcctgaa gaactcccgt gacggcaacc ctcgaaaatg
     1021 gcgattcgat gcaacaaaca gtgaactatg tgccgaacgt cacgaatgct gaaatcacgc
     1081 tggcagcctc gaaggatccg gtgattgccg acaataacga tctcacgaca ctaacagcac
     1141 cgtcgctgat acagagggca atgcgatagc caacactgag gtaacattta ctctaccgga
     1201 agatgtgaag gcgaacttca cgctgagcga tggcgacaaa gcggttactg atgccgatgg
     1261 caaagcgaaa gtcacgctga aaggtacaaa agcaggcgct cataccgtga cagcatcgat
     1321 ggttggcggt aagagtgagc agttggtggt gaactttact gcggatacgc tcactgcgca
     1381 ggttaatctt aacgttaccg aggacaattt catcgccaat aacattggaa tgaccaggct
     1441 acaggcaaca gtgactgatg gaaacggcaa cccagtagaa ggtataaaag ttaatttccg
     1501 cggaacctcc gtcacgctaa gcagcaccag cgttgaaacg gatgatcagg ttttcgctga
     1561 aattcttgtg acaagcaccg aggtcggact aaaaacagtt tcagcctctc tggcagataa
     1621 acctactgaa gtcatctcgc gattactgaa tgccaaggta gatgttaatt ctgcgacgat
     1681 taccagtcag gagatacctg aaggtcaggt catggtcgca caagacatag cagttaaagc
     1741 tcacgttaac gaccagtttg gcaacccggt gacgcatcaa cccgcgacat tcagtgcagc
     1801 gccatcctcg caaatgatca tcagccagaa tacggtctct actaatacgc agggagtagc
     1861 cgaggtcacc atgacgcctg aaagaaacgg ctcgtatact gtaaaagcat ccctagcgaa
     1921 tggagcttca cttgagaaac aactggaggc tattgatgaa aaactgacac tcacgtccag
     1981 tccgcttatc ggtgtcaatg ctcctaaagg cgctactctg acggcaacgc taacctctgc
     2041 aaatggcact ccagtggagg gtcaggtcat caactttagc gtaacgctag aaggtgcgac
     2101 gttaagtggc ggaaaagtga gaaccaactc ttcaggtcag gctccagtcg ttctgaccag
     2161 caataaagtc ggtacatata cggtgactgc atcgttccat aacggcgtca caatacagac
     2221 acagacaacc gtgaaggtta ctggcaaccc aagcaccgca catgttgcta gctttatcgc
     2281 tgatccgtcg actatcgccg ccaccaacag tgatttaagt accttaaagg caacggttga
     2341 ggatggcagt ggtaacctga tcgaaggtct cactgtgtac ttcgccttaa aaagcggctc
     2401 caccacatta acgtcattaa cagcggtgac cgatcaaaac ggaatcgcga caacaagcgt
     2461 gaaaggtgag ataacgggga gcgtcactgt aagcgcagtc acaagcgcag gtggaatgca
     2521 aacagtagat atatcgctag tggcaggccc agcagacgcc tcgcagtcca tccttaagaa
     2581 caaccagtca tcactgaaag gggactttac cgatagtgct gagctacatc ttgttctgca
     2641 cgatatatca ggcaatccga tcaaagtttc ttaagggata gaatttgtgc aatcaggtac
     2701 aaacgtgccc tatatgaaaa ttagcgcaat tgattacagt caaaatatca acggtgatta
     2761 caaagctact gttacaggag gcggagaggg tatcgcaacg ctgatccccg tattgaatgg
     2821 tgttcatcag gcgggtctga gtaccacgat agaattcatt agcgcggaaa ccagaccaat
     2881 gacgggtaca gtgtcagtga atagtgcaaa cctaccgaca gcttcattcc cttcgcaggg
     2941 atttaccggg gcgtattatc agttgaataa tgacaacttt gcaccaggaa aaacggcggc
     3001 tgattattcg ttttcaagct cggcctcctg ggtaggcgtt gatgctactg gtaaagtgac
     3061 attcaaaaat gacggtgata gcaatactgt aataatcaca gcgcccccaa gatccggtgg
     3121 tgctatttac caaacagttc ctcctgaatc gcgttcggtg tgactatgat cattttcggt
     3181 gagtattgct ttgatccaaa ggctggtttt ctgtgatgat tgaccagcct cagagtattg
     3241 atgacaccac cgttcataac agatcggcat tactatgcat aagtactgcc tcaaattctc
     3301 gccaaaacac tttttaatgc ctttgcgaat tcatccagac ttatattgtc aattttttgc
     3361 ccaacgacga ctgactccga aacatcggcc attgcgggcc atcgcgtttt gtaatcagca
     3421 taaattgcaa caacaggggt attataggca cttgccatat gtaatattga ggtgtcagga
     3481 gttattgcaa tataagcatc ttttatgatt gctgctgaac gtttaatcga gggtgataag
     3541 gataaacggt agacattatt ataacaatcc actaatgccc tggctttatc ttcccctttg
     3601 ggtccataaa cgatcactat tggaatatct gtttcacttt gaatttttgc tattagattt
     3661 tctgcaatcg ataatgaaaa tgtacgttcc tgcgagctac cgtctaaatt gagcgcaatg
     3721 taagatccta acgatcgcat ttcctcgcgt acctcatcga gtacatcatc gcttaatggc
     3781 aattcatata ttgacctgac tgccggaaaa cccgcctcac gcatcataaa tgcccatgtc
     3841 atgggtacag gagtccggag attctgatcc atccgggacg cgttcttaca caaaggggaa
     3901 tagcacttca tggttaaacc gacgacctgg aaattcgttt ttgctttcaa ctgactgata
     3961 aatatcatcg ttttcaggtt ctttttacgc atcgcctcta tacaaagatc cggcgtaccg
     4021 tattgcagtg ttatgttctt cgctaaatct tttatttctt ttaatgttgc gtgatcctgc
     4081 atagtcattg tgactaatgt taatttagtc tgttcaagtt tgagcgcatt aaagacttct
     4141 aaattaattg tcgacgtaac aattaataga tgcttaattt tatgcaattc aagcgcccga
     4201 ataacaggaa agatggccat agcatcgcca atctgatcgg gaatatgtat gacaacaaag
     4261 tctgtttttt caatattaaa attataagct ttataatcgt agtaactaaa tgcaatacgt
     4321 ctcaacaatg atactaaaaa cataactaac ctcgcctccc tactggttat aatgcaatgc
     4381 agtctatcag aatcatcagg gtgcaatttt gtgcatatgc ggacttttat gtttcatatc
     4441 tcgaacctgt gggccctctg cttaatcctt aaacaacacc agcaactcct gcgctttcat
     4501 cttccatcga atttttcatg ttgccgctaa tcagccataa aatcatttgc agatgcgctc
     4561 tgtcgaggta gtctcataag gttcgtttat agatcgacgg caatgtgagt taccttttcc
     4621 atactaatta taaaaagaca gtacaaacag gatcattatg gactccacgc tcatctccac
     4681 tcgtcccgat gaagggacgc tttcgttaag tcgcgcccga cgagctgcgt taggcagttt
     4741 cgctggtgcc gtcgtcgact ggtatgattt tttnnnnnnn nnnctactct atggcattac
     4801 cgccgcactg gtgtttaatc gcgagttttt cccgcaagta agcccggcga tgggaacgct
     4861 cgccgcattt gccacctttg gtgtcggctt ccttttccga ccgctcggcg gtgtcatttt
     4921 cggtcacttt ggtgaccggc tgggacgtaa gcgcatgtta atgctgaccg tctggatgat
     4981 gggcatcgcg acagccttga ttggtattct tccttcattc tcgaccattg ggtggtgggc
     5041 acctattttg ctggtgacac tgcgtgccat tcagggattt gccgtcggcg gcgaatgggg
     5101 aggcgcagcg ttgctttccg ttgaaagtgc gccgaaaaat aaaaaagcct tttacagtag
     5161 cggtgtacaa gttggctacg gagtaggttt actgctttca accggactgg tttcattgat
     5221 cagtatgatg acgactgacg aacagttttt aagctggggc tggcgcattc ctttcctgtt
     5281 tagcatcgta ctggtactgg gagcattgtg ggtgcgcaat ggcatggagg agtccgcgga
     5341 atttgaacaa cagcaacata atcaagcggc cgcgaaaaaa cgcatcccgg ttatcgaagc
     5401 tctgttacga catcccggtg ctttcctgaa gattattgca ctacgactgt gcgagttgct
     5461 gacgatgtac attgttactg cctttgcact taattattca acccagaata tggggttacc
     5521 gcgcgaactt ttccttaata ttggtttgct ggtcggtgga ttaagctgcc tgacaattcc
     5581 ctgttttgcc tggcttgccg atcgttttgg tcggcgcagg gtttatatca caggcgcgtt
     5641 gatcggaacg ttgagcgcat ttcctttctt tatggcgctt gaagcacaat ctattttctg
     5701 gatagttttc ttctccataa tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca
     5761 acaaccgatg tttaccgaaa tgtttggtgc cagttatcgc tacagtggtg ctggagtcgg
     5821 ttatcaggtt gccagtgtgg ttggcggtgg atttacgcct tttattgccg ctgcactcat
     5881 cacttacttt gccgggaact ggcatagcgt cgctatttat ttgctggctg gatgcctgat
     5941 ttccgcaatg accgctttgt tgatgaaaga caatcaacgc gcttaatagc ctggcgaaga
     6001 tcatccgatc ttcgccttac acttttgttt cacattcctg tgacatacta tcggatgtgc
     6061 ggtaattgta tggaacagga gacacacatg aataataagg gctccggtct gaccccagct
     6121 caggcactgg ataaactcga cgcgctgtat gagcaatctg tagttgcttt acgcaacgcc
     6181 attggcaaat atattacaag tggcgaatta tctgatgaaa acgcccgcaa acaaggtctt
     6241 tttgtctatc catcactgac cgtaacctgg gacggtagca caactaatcc ccccaaaacg
     6301 cgcgcatttg gtcgttttac tcacgcaggc agttacacca ccacgattac tcgccctact
     6361 ctctttcgtt cgtatctcaa tgaacaactt acgttgctgt atcaggatta tggtgcgcat
     6421 atctctgtgc aaccctcgca gcatgaaatc ccttatcctt atgtcatcga tggctctgaa
     6481 ttaacacttg atcgctcaat gagcgctggt ttaactcgct acttcccgac aacagaactg
     6541 gcgcaaatcg gcgatgaaac tgcagacggc atttatcatc caacggaatt atccccgtta
     6601 tcgcattttg atgctcgccg ggttgatttt tccctcgcac ggttgcgcca ttataccggt
     6661 acgccagtag aacattttca accgttcgtc ttgtttacca actacacacg ttatgtggat
     6721 gaattcgttc gttggggatg cagccagatc ctcgatcctg atagtcccta cattgccctt
     6781 tcttgtgctg gcgggaactg gatcactgcc gaaaccgaag cgccagaaga agccatttcc
     6841 gaccttgcat ggaaaaaaca ccagatgcca gcatggcatt taattaccgc tgatggtcgg
     6901 ggtattactc tggtgaatat tggcgtggga ccgtccaatg ctaaaaccat ctgcgatcat
     6961 ctggcagtgc tacgcccgga tgtctggttg atgattggtc actgtggcgg attacgtgaa
     7021 agccaggcca ttggagatta tgtccttgca cacgcttatt tacgcgatga ccacgttctt
     7081 gatgcggttc tgccgcccga tattcctatt ccgagcattg ctgaagtgca acgtgcgctt
     7141 tatgacgcta ccaagttagt cagcggcagg cctggtgagg aagtcaaaca gcggctgcgt
     7201 acaggtactg tggtaaccac agatgacagg aactgggagt tacgttactc agcttctgca
     7261 cttcgtttta acttaagccg ggccgtagca attgatatgg aaagtgcaac cattgccgcg
     7321 caaggatatc gtttccgcgt gccatacggg acactactgt gtgtttcaga taaaccgtta
     7381 catggtgaga ttaagcttcc cggccaggct aaccgatttt atgaaggcgc tatttccgaa
     7441 cacctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat
     7501 tcacgtaaat tacgaacctt taatgagccg ccgttccgat aataagttat gaaccttctt
     7561 atcagaaata gatgtgaagg attaagtaag acatctggca aatagcctgc aataaacgcg
     7621 ggctgtttgc ttccttgggc agatgcga
//
LOCUS       DAWDRC010000123         7589 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4649_length_7589_cov_2.089461, whole genome shotgun sequence.
ACCESSION   DAWDRC010000123 DAWDRC010000000
VERSION     DAWDRC010000123.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4649_length_7589_cov_2.089461"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2968..2977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3411..3420
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6147..6156
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1853 a         2038 c         1999 g         1669 t
ORIGIN      
        1 gtgccacggt ctggctgccg aggggtatcg cgtggcggtt gtcgatattc agagcgacaa
       61 agccgcaaat gtggcacaag aaattaacgc cgaatatggt gaaggaacag cgtacggttt
      121 tggtgctgac gccaccagcg agcaaagcgt tctggcgctt tctcgtgggg tagatgaaat
      181 ctttggtcgc gtggatttgc tggtctacag cgccggaata gcaaaagcag cctttatcag
      241 cgacttccag ctcggcgatt ttgaccgttc gctacaggtg aatctggtgg gttatttcct
      301 gtgtgcccgt gaattttcgc gtttgatgat ccgcgacggg attcagggtc gcattattca
      361 gatcaactcg aaatccggca aagtgggcag caaacacaac tctggctaca gcgccgcgaa
      421 atttggtggc gtcgggctga ctcaatcact ggcgctggat ctggcggagt acggcattac
      481 ggtgcattcg ctgatgctcg gtaacctgct gaaatcgcct atgttccagt cgctattgcc
      541 acaatacgcg accaagctgg gtatcaaacc ggagcaagtc gagcagtatt acatcgataa
      601 agtaccgctc aaacgcggct gcgattatca ggatgtgctg aatatgctgc tgttctacgc
      661 cagtcctaag gcgtcgtact gcactggtca gtcgatcaat gtcaccggcg gtcaggtgat
      721 gttctgatca acagcggaga tcttttaagg atctccgcgc gactaataga atgcctgatg
      781 cgctacgctt atcaggccta caggacttcc gccactacat tacggagaag ttatggtatc
      841 cgcactcatc accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggttggca
      901 aatttctcgt tttaaccgtg ccttcgacac actatgccaa caagggcgcg ttggcgtggg
      961 acgttccagc gggcgcttta aaccgcgggt cgtggtcgct attgcgctgg acgatcagca
     1021 gcgcatcgtc gacaccttgt ttatgaaagg actgaccgtc ttcgcccgac cgcaaaaaat
     1081 tcccgcaatt accggcatgc atgtgggtga tttacagccc gatgtgatct ttccccatga
     1141 tccactatca cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg
     1201 tgaatgttac ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat
     1261 gaaacctcgt cagcgtcagg ccgccattct ggagtatcta caaaagcagg gtaaatgctc
     1321 ggttgaagaa ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt
     1381 cattctggaa catgccggaa ccgtcattcg tacttatggc ggcgtggtgt tgaacaaaga
     1441 ggaatccgat ccgcctatcg atcataaaac gcttatcaac acccacaaaa aagagctgat
     1501 tgcagaagct gccgttggtt ttattcatga tggcgactca ataattcttg atgctggcag
     1561 taccgtgttg cagatggtgc ctctgctatc gcgctttaat aacatcacgg tgatgaccaa
     1621 cagcctgcac atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc
     1681 aggcggaaca tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt
     1741 cgagcatttc accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg
     1801 cgtaaccacc tttaacgagg tctataccgt cagtaaggcg atgtgtaatg ccgcgcgcga
     1861 agtgattttg atggctgact catcgaagtt tggtcgtaaa agccccaacg tggtttgtag
     1921 tcttgaaagc gtcgataagc tgattaccga cgcaggtatc gatccagcgt ttcgtcaggc
     1981 gctggaagaa aaagggatcg acgtgatcat aaccggagag agcaatgagt gaagcactac
     2041 tgaacgcggg acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccagaac
     2101 gtctgggcga tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg
     2161 tggtttcggg aattggcaaa tcgggccaca ttggtaagaa aatcgcggca acgcttgcca
     2221 gcaccggcac accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga
     2281 tgattgaaag ccgcgatgtg atgctgttta tctcttattc cggtggcgcg aaggaactgg
     2341 atctgattat tccgcgtctg gaagacaaat ctatcgcgct gctggcaatg accggaaaac
     2401 cgacgtcacc gctgggcctg gcggcaaaag cggtgctgga tatctccgta gaacgcgaag
     2461 cctgcccgat gcaccttgcg ccgacttcca gcaccgtgaa taccctgatg atgggcgacg
     2521 cgctggcgat ggcagtcatg caggcgcgtg gttttaatga agaagatttt gcacgctccc
     2581 accctgctgg agcactgggc gctcgcttgc tgaacaaagt gcatcatctt atgcgtcgtg
     2641 acgatgccat cccgcaggtg gcgttaaccg ccagcgtgat ggacgcgatg ctggaactta
     2701 gccgcaccgg tttaggtctg gtggcagtgt gcgatgatca acgactggtg agaggcgtct
     2761 ttaccgacgg cgatttacgt cgctggctgg ttggcggcgg cgcactcacc acgccagtca
     2821 atgaagcgat gacggtcggc ggcaccacgt tgcaatcgca aagccgtgcc atcgacgcca
     2881 aagaaatcct gatgaaacgc aaaatcaccg ccgcaccggt agtggatgaa aacggcaaac
     2941 tcaccggcgc aattaacctg caggattnnn nnnnnnntgg ttgtacactc tgctttggtg
     3001 gcaagcagtg cctgacgaat ggtttcacgc tggaacgctt ctgtcgcttc acgcaggttt
     3061 tgcttaacaa cggtcaccgc cgccgcttct ggcggcggca acgtcacctc aggaaaagca
     3121 aaatgttgcg cctcaagaat cacttcatcg ccgctgcggg ttgctctcgc cagcactacc
     3181 gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta gtgttgcagt
     3241 aaatttcgcg ctccggcact taataccacg cgggagagcc ccagccgcaa acgacactgc
     3301 tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag cggcggcact
     3361 gaaagtggaa acacgctcag gcggtggaac aagtcagcgc gaaatcgccc nnnnnnnnnn
     3421 caacgcgttg aatatcgcca tactgcaaca cccttagcag cttggcctgc aatgccaacg
     3481 acaactcgcc gatctcatcg agaaacagcg taccgttatc cgccatttca aacttcccgc
     3541 tgcggttact gatagcacca gtaaacgccc ctttcacatg cccgaacaac tcactttccg
     3601 ccacactttc cggcagtgcg gcacagttga gatagaccag cggattcacc gcccgtggcg
     3661 aggcttcatg aatcgctttc gccaccagct ccttaccggt tcccgtctca ccgctaatta
     3721 aaacgttgag atcggatgcc gccacaatct caatctcttt tttcaattgc gtcatgcctg
     3781 gcgacagacc gatcatctgc gtctgtttca ccgcttcaaa cggcgcggct tcgcctggca
     3841 gcatattctg gctttccagt tgttcaatca gtaacgcatt gcttaacgct cccgccgcca
     3901 gcgcagcaat caggcgtaac tcttcgtcgc tgaaaacatc gaactgatcg ggctgcatcc
     3961 cgtcgagcgt caaggcaccg atcaggtttt gcccggcaaa caacggcaaa ccaacgcagg
     4021 cgtgaacctt cagactctcc tgccctggaa tcagaccgtc ataaggatcg ggcaattcgc
     4081 tgtctgcggg aaagcgcacc acatcacccg cgcgggcaat cgcttccagc cgtggatgcc
     4141 cttccagcgc aaagcgtcta ccgagtacat ccttcgccag cccgtcgatg gcaagcggaa
     4201 taaactgccg cgagtcgtaa cgtagcaacg cagacgcatc gcactccagc acctgacgca
     4261 gcgtggtgat caggcgctga aaacgatcct gatgaccaat cccacgctgc aattcgatgg
     4321 cgatattcgc cagcacatca acggaaaaac tcatctttgc ctcactgtca atttgactat
     4381 agatattgtc atatcgacca ttttattgat agtcattttg actactcatt aatgagcaca
     4441 attttattta tagagtaaaa acaattagat aaaaaactgg cacgcaatct gcaatcagca
     4501 agacatcttt ttagaacacg ctgaataaat tgaggttgct atgtctattg tggtgaaaaa
     4561 taacattcat tgggttggtc aacgtgactg ggaagtgcgt gattttcacg gtacagaata
     4621 taaaacgctg cgcggcagca gctacaacag ctacctcatc cgcgaagaaa aaaacgtgct
     4681 gatcgacacc gtcgaccata aattcagccg cgaatttgtg cagaacctgc gtaatgaaat
     4741 cgatctggcg gatatcgatt acatcataat taaccatgct gaagaggacc acgccggggc
     4801 gctgaccgaa ctgatggcac aaattcccga tacgccgatc tactgtactg ccaacgctat
     4861 cgactcgata aatggtcatc accatcatcc ggagtggaat tttaatgtag tgaaaactgg
     4921 cgacacgctg gatatcggca acggcaaaca actcattttt gtcgaaacgc caatgctgca
     4981 ctggccggac agcatgatga cttacctgac aggcgacgcg gtgctgttca gtaacgatgc
     5041 tttcggtcag cactactgcg acgagcatct gttcaacgat gaagtggatc agactgagct
     5101 tttcgagcag tgccagcgtt actacgccaa tatcctgacg ccgttcagcc gcctggtaac
     5161 gccgaaaatt accgagatcc tgggctttaa cttgccagtc gatatgatag cgacctctca
     5221 cggcgtggta tggcgcgata acccgacgca aattgtcgag ctgtacctga aatgggcggc
     5281 ggattatcag gaagacagaa tcaccatttt ctacgacacc atgtcgaata atacccgcat
     5341 gatggccgat gctatcgccc agggtattgc ggaaaccgac ccacgcgtgg cggtgaaaat
     5401 tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc gctcaaaagg
     5461 cgtgctggtc ggcacttcta cgatgaataa tgtgatgatg ccgaaaatcg ccgggctggt
     5521 ggaggagatg accggattac gcttccgtaa caaacgcgcc agcgctttcg gctctcacgg
     5581 ctggagcggc ggtgcggtgg atcgtctttc cacgcgcctg caggatgcgg gtttcgaaat
     5641 gtcgcttagc ctgaaagcca aatggcgacc agaccaggac gctctggagt tatgtcgtga
     5701 acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg ctgccacaga gcacggtgaa
     5761 tacggtggtt gaagaagaaa cctctgccgc cacgacggct gacctcggcc cacggatgca
     5821 atgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc aggacgttgc
     5881 gccaggaacg ccgtggagtg aagtcccgga taacttcctg tgcccggaat gctctctcgg
     5941 caaagacgtc tttgacgaac tggcatcgga ggcaaaatga gcaacggcat tgtgatcatt
     6001 ggttcgggct tcgccgcccg ccaactggtg aaaaatattc gcaaacagga cgccagtatt
     6061 ccattaaccc tgattgccgc cgacagcatg gatgagtaca acaaacctga cctcagccat
     6121 gttatcagtc aggggcaacg tgccgannnn nnnnnnccga cgaccttgtc cgccagacgg
     6181 caggtgaatt ttccgagcag tttaatctgc gcctgtttcc gcacacctgg gtaacggata
     6241 tcgatgccga agcccatgtg gtgaaaagtc agaataatca gtggcaatac gacaagttag
     6301 tgctggcaac cggtgccagc gcctttgtcc cgccagtgcc cgggcgtgag ttaatgctga
     6361 cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca actgcgggat gcccgacgcg
     6421 tgttgattgt cggcggtggt ttgattggta gtgaactggc gatggatttt tgtcgggcag
     6481 gcaaagcggt cacgctaatc gacaacgctg ccagtattct ggcgtcgtta atgccaccgg
     6541 aagtcagcag ccgcttgcag catcggttga cggagatggg cgttcatctg ctgttgaaat
     6601 ctcagttgca gggactagaa aaaacagatt ctggcattct ggcaacgctg gaccgccagc
     6661 gctgcatcga agtggatgcg gtaattgccg ccaccggact gcgcccggaa accgccctgg
     6721 cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca
     6781 gtaatgccga tatttatgcg ctgggcgatt gcgcggaaat taacggtcag gtattgccgt
     6841 tcctccagcc gattcaactt agcgcgatgg tgctggcaaa aaatcttctc ggcaataaca
     6901 cgccgctgaa actcccggcg atgctggtga aaatcaaaac gccggaatta ccgctgcatc
     6961 tggcaggcga aacccagcgt caggatttac gctggcaaat taataccgaa cgccagggaa
     7021 tggtggcgcg cggcgttgac gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc
     7081 ggatgaaaga ggcatttgga ttgttgaaaa cgttgtcgat gtaggcgagc tacagtgccc
     7141 caaatgtcgg atgcggcgct gacgcgtctt atccgaccta cagggaacgc atgtgttgat
     7201 cacataatac gtttatgtcg catcctgcac tgtcccaaaa tgtcggatgc gacgctggcg
     7261 cgtcttatcc gacctgcggg gatgaatatg taggccggat aaggcgttca cgccgcatcc
     7321 ggcaatggtg tccaaatgca gcacgtttta tccgttctgg acttcccctg ccaaccaacg
     7381 cgccgcaaca ataaccccct gccccagaga caacccgcca tcgcccgcag gtaaactttg
     7441 tgggaagagc aacgtaaaat cagcaagata atgcgccaga cgtgcacgca gcaaacagtt
     7501 atgaataact ccgccgctaa ataccagcgt agtgataccc cgcatcgttg cctgctcacg
     7561 cctcaacgcg gcaaaaccct gcgccagcc
//
LOCUS       DAWDRC010000124         7579 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4657_length_7579_cov_2.363105, whole genome shotgun sequence.
ACCESSION   DAWDRC010000124 DAWDRC010000000
VERSION     DAWDRC010000124.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4657_length_7579_cov_2.363105"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    7251..7260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2002 a         1810 c         1874 g         1883 t
ORIGIN      
        1 acatgccggg ttatcgtaaa gcgtttaaag cgattaagtc gctgattact gacgtcagcg
       61 aaacgcacaa gatcagcgcc gaattgctgg catcgcgtcg gcaaatcaac caactgctga
      121 actggcactg gaaactgaaa ccgcagaaca atttgccgga gctgatttcc ggctggcgtg
      181 gtgagctgat ggcggaagca ttacacaatt tattgcagga atatccacag taaaatcttc
      241 cgaagccgga ctgggcgcgc tcagtccggc ttcggcaaac tacaaatcca acagcaggcg
      301 gttcaaatga acttatttga gcttaacgca aaatgcgctc gacaccacga cgagtaaata
      361 ccgattcctg aatgacgact tcattctctg ttccttcatt cactctaccg cctggcgagg
      421 aatattcgcc ttcggtgttt ttccggacca cgtaaaaatc gatgtcgcca ggctgtttcc
      481 cgccagtggt catttagtac aagtgctacc ggaatattac cagccagcga atgtctgggc
      541 cgtttatgtt tcaaggctgg cgacgtcagc gaaagtgcgg ataacggtag aatttttacg
      601 ccagtatttt gccgagcact acccgaattt ttcactggag catgcctgat taatgattca
      661 attatcgggt tgatatcgag ttaaaacctg attttctcct ttctaagccg ctacagattg
      721 gttagcatat tcacctttaa tcgcgcatga tcgaaagata attaaagagg ttaatgtgtt
      781 cgctgaatac ggggttctga attactggac ctatctggtt ggggccattt ttattgtgtt
      841 ggtgccaggg ccaaataccc tgtttgtact caaaaatagc gtcagtagcg gtatgaaagg
      901 cggttatctt gcggcctgtg gtgtatttat tggcgatgcg gtattgatgt ttctggcatg
      961 ggctggagtg gcgacattaa ttaagaccac cccgatatta ttcaacattg tacgttatct
     1021 tggtgcgttt tatttgctct atctggggag taaaattctc tacgcgaccc tgaaaggtaa
     1081 aaatagcgag accaaatccg atgagcccca atacggtgcc atttttaaac gcgcgttaat
     1141 tttgagcctg actaatccga aagccatttt gttctatgtg tcgtttttcg tacagtttat
     1201 cgatgttaat gccccacata cgggaatttc attctttatt ctggcgacga cgctggaact
     1261 ggtgagtttc tgctatttga gctttctgat tatttctggg gcttttgtca cgcagtacat
     1321 acgtaccaaa aagaaactgg ctaaagtggg caactcactg attggtttga tgttcgtggg
     1381 tttcgccgcc cgactggcga cgctgcaatc ctgatgcttt cagcccgcgt tgccgcgggc
     1441 ttcccacctg taatcctccc tgattcttct ctgatacggt gctaaaagta accaataaat
     1501 ggtatttaaa atgcaaatta tcaggcgtac cctgaaacgg ctggaataaa ccgttttcag
     1561 cgcattcacc gaaggaggga aaaggatgct gcaaatccca cagaattata ttcatacgcg
     1621 ctcaacgcct ttctggaata aacaaactgc gcctgccgga atattcgaac gtcatcttga
     1681 taaaggaacg cgcccggggg tttacccacg cctttccgtt atgcatgggg cggtcaaata
     1741 tctcggctac gctgatgaac acagtgcaga gcctgatcag gtgatcctta tcgaagcggg
     1801 gcagtttgcg gtgttccctc cagaaaagtg gcacaacatt gaagccatga ctgacgatac
     1861 ttatttcaac attgacttct tcgttgctcc tgaagtcctg ctggaaggcg cgcaacaacg
     1921 gaaagtcatt cataacggga aatgaatcat gggcaaagca acgtataccg tgaccgtcac
     1981 caataacagc aatggcgttt ctgtcgatta tgaaacagag acgccgatga ctttgctggt
     2041 gccagaagtg gcggctgaag tgataaaaga tctggtgaat accgtgcgtt cttatgacac
     2101 ggaaaacgaa catgatgttt gtggttggta attatcactg atcgttgatg gcgcaatttc
     2161 tgcgccatcg caaaaaattc ctccgtgatg ctcgtcacaa atcttaccct cccgatgttg
     2221 tgctatgctt tttatcagcg actaacccgc taatgcaaaa cagataagtg aggaaacaat
     2281 gggcattctg tcatggatta tttttgggct tattgccggt attctggcga agtggatcat
     2341 gccaggtaaa gatggtggtg gattctttat gactatcctg ctggggatag tcggtgccgt
     2401 agtcggcgga tggatcagca cgctgtttgg ctttggtaaa gtcgatggct tcaattttgg
     2461 cagcttcgtg gttgccgtta ttggtgcgat tgttgtgcta tttatctaca ggaagattaa
     2521 aagttaacgc gtaaattgca caaaggctgc acacaggcag cctttgctat tttttagatg
     2581 tgacgttacc accatcccag ctcagtgccg agtacgacaa taatcgccac acccatcata
     2641 ataatcgttg tttttttcat ctttatgctc ccggggcagc atagccgcca ataaaaaacc
     2701 atgctaaaaa tacgaccgct gtaataaaga ttacaaccgg aaaaataata ccgattcgca
     2761 tgttatcacc aatcaaaagg atgtgaatac gcctcaggaa tgtagcgctg aatgcgtgac
     2821 aaaaggtgtt ttctgtttat gttttttctc ctgatacatc gcgatatcgg cagcatgcag
     2881 ggcactgtct gcatcggtcg tttcaggatc gacttctact atgccaagac tggcaccagg
     2941 ataatacaaa tcaacatcac ctaagtgata ttctccgcgt atttgttgtt gaatacgttc
     3001 tcgcagcgac gaaatatccg cattctcgtt gttcagtgaa acaaccaaaa actcatcacc
     3061 gccgagacga ccaataactt cgccattttg ctggagcgta ttaaggcgct cgccaacctg
     3121 aatgagaaac agatcgccac tattatgacc aaaacgatca ttgattaatt tgaagttatc
     3181 cagatcgata aacgcgatca ttatcttatg gttaagatgc cgggcgagag aaaacagcgt
     3241 cgtcagattt tcaaaaatcg cccgccgatt cggtagcccg gttaacgagt cggtatacga
     3301 ttgcgcaatc agtccggcga acaactgtaa gacctgttcc gcgcgttcac tccactggcg
     3361 cttctcactg ctggcggcgc aaagcgtgcc atagaatgat ccatccggta agtgaattgg
     3421 cgtgctcaga aatgtggtga tgccaagatt gcgtgccgca acacagtcgc cccaacggtc
     3481 ggggacttca tcgctgaaaa aacagttttc atcaatggcg cgtttgcata acgagtaatc
     3541 ccacgagacg gtaaaattct ccgggatgtg cattttctgg ctattacggg caaacattat
     3601 atgctgcagg cgcgcttcga catccacttt ggtcaggtag gttgactcca tatcagtgac
     3661 catttccagc atctccagaa gctgtcgaac cagactttcc tgtgactgtt ctttggcaag
     3721 ggattgcgag acgcgggcga taatctgatc tgacacgaaa caactccagg atacggcggg
     3781 taaatgctga ttatttaatc tttgcaggca tgataaaaca tcatcaacaa agatgaaaca
     3841 aggcgtaata tgttttgagt gaatgtaata aaaatagtgc tgcaaaacaa aacgataccg
     3901 cgatactcta agcttggata aatatgtgag atcaataaat gaaaattatc agttttgttc
     3961 tgccttgctt gctggtcctg gctggttgtt caaccccttc tcagccagaa gcacctaaac
     4021 cgccgcagat tggtatggca aatccggcgt cggtctattg ccagcagaag ggcgggacgc
     4081 tcattcctgt gcagacagcg caaggggtca gcaacaattg caaattaccg ggcggtgaaa
     4141 cgattgatga atgggcattg tggcgacggg accatccggc tggtgaaaaa taaccgccgg
     4201 atgctgtact aaaatcacaa gctacgtagc cagtctgcca gcaccagcgc atggttctgc
     4261 gtggtgtttt ttgctgagta gagcagggtc agcggctgtt ttttggcgat gtctgccagc
     4321 cgctttcctt cttgctcgtg ttgcgccagt tctgcaagat attgctcgcg aaaggttgca
     4381 aaatcgacga cttcgccgtg aaaggctttg cgcagctccg ttgacggcgt gatttcttta
     4441 tcccattcat caagggctaa atcggttttt ttgataccgc gtggccagag gcggtcgacc
     4501 agtacgcgat aaccatcgct ctgttcagcc ggatcataaa cgcgtttgca ctgaatattc
     4561 atcgtatttg ttctcccttt cacgcatgat ttgtcacgtc actatagcac cggatattag
     4621 cgaatttctt tgtctctccc ggcgcaaagt ccgaaaatcg ccatcagtag tgaaagtatt
     4681 gcaacaccca gcagtggtac ggaccagtca ccgttagcat cgtgtatttt acccatcagc
     4741 ggcggcccac aggctgccag caaatacccg acggattgtg ccatccccga gagtgccgcc
     4801 gcctgatgcg cagaacttgc ccgcagacca atgaacgtca accccagtat cattgttgcg
     4861 ccggaaccaa aaccgaaaag cagagtccaa gtgatcgcgt gtgctggcat aaagcagagc
     4921 ccaaccgcgc ccactgcgca catcaaggca acgaacgctg caataccacg ctgatctttc
     4981 acatgatgta agaaaagtgg gatcagcaaa ccgggagctg ctgtggctag ttgcagcaaa
     5041 ccatgcagtg aacccgcctg tgcttcgcta tagccgtgac tgatgaggat cgccggaagc
     5101 cagccaataa tcacgtaata gaccagtgag ttgatcccaa gaaacaatgt tacctgccag
     5161 gcaagcggcg aacgccagat accccgagta tgtaaggcgc gtgcggtgct caaatttgaa
     5221 tgttgttggc ttcgcgactg tggcagccat aaaaaaagag ccagcagagg aaaacacatc
     5281 agcatgagca acgcgcctcg ccagccaaaa ccgttcaaag ccagcggcac taccatcgcc
     5341 gatcccagtg ccgctgcagc tcccattgtc agggaatatg cgccggtaag tctggcgacg
     5401 gaatgaggga aatcgcgttt aattaatcct ggcagtaaga cattgcctaa tgcaatccca
     5461 ccgccaatga tcgctgtacc gccaaataat aagtaaggcg aagggagaga gcgaattgcg
     5521 atacctgcac agatcaaaag taacgcggca aacaggctac gttccatacc aaaacgtcgc
     5581 gccaccgggg cggccagcgg tgagattagc gcaaaggcta ataatggcag ggtggtcaat
     5641 aagccggttt gcgccgtcgt tagcgagtaa gcggaacgaa tcgtatccag taacggtgct
     5701 gcgccggtaa aggtgacgcg taatgttgtg gcaatcatca gaatgccagc gataaggaaa
     5761 atcctgtttt taccgcttaa tgaagttgaa caggtcatat gcttctcatt gttacaaacg
     5821 aggctgtacc ttaccgtttt tattgttatg ttgaatcaag ctaaaatgac agaatatcgc
     5881 taaattcgga caaaatgatg cacaggttga atctcaatgg ctatgaaccc gaccgacacc
     5941 atgaagccgc ggtcgcattt tgtattcacg ccggaacgga tgagttaacc agtcctgtac
     6001 atcagcaccg caaagggcag ttgatactgg cgctacatgg tgcaattacc tgtacggtgg
     6061 aaaatgcttt gtggatggtg ccgccacaat atgcggtttg gataccgggc ggggtgaaac
     6121 atagtaatca agtgacagct aacgctgaac tctgttttct gtttatcgag cccagcgcag
     6181 tgacaatgcc gacaacatgt tgcacattga aaatatcgcc cttatgccgg gaattaattt
     6241 taaccctggc aaacaggacg acgactcagc gtgctgagcc gatgacccgc agattaattc
     6301 aggtcctgtt tgatgaactc cctcagcagc cacaacaaca gctgcacctg cccgtttctt
     6361 ctcatcctaa aatccgcacg atggtggaga tgatggcgaa agagcccgtc gagtggggct
     6421 cattggggca atgggctggc ttttttgcga tgagtgaacg caatcttgcc cggctgatag
     6481 tgaaagagac ggggttgagt tttcgccagt ggcgtcagca gttacagctg attatggcgc
     6541 ttcagggatt agtgaaaggc gatactgtac agaaagttgc tcatacgctg gggtatgatt
     6601 caacaacggc ctttattact atgtttaaga aaggcctggg gcaaacgcca ggccgatata
     6661 ttgcgggatt aactactgtt tccccacaat cagcgaaacc agacccgcgg caataagcgg
     6721 gccaaccggt acgccgcgaa acagcgccac gcctaaaaca gtgcccacca gcagcccggc
     6781 gaccagttgc ggctggctgc ccattaacgt cacgccgcgc ccccccagcc aggagacaat
     6841 aacgccgact gcaattgcca ccagcgattt ccagttcaga aacgagtgaa tcagggttga
     6901 gggcggtagc gtcccgctgg cgatgggagc cataacgcca atcgtcagaa tgattatccc
     6961 gatacttagc ccctgttttt caatccacgg gaaaaaggtg cttaacggtg tgacgcggac
     7021 aatgatcaac accagaattg aaacggcgac agtggtgtta tgactgataa agcccagcgc
     7081 agctaacccg agcaggatca gcaaagtgac atcaaacatc gtggtttcct tgccaaaaaa
     7141 taataagcct gcttacttta cgcttgctgc aggcaggaag tagaaaatct ttacgttttc
     7201 tgcataaaaa atcaggcgaa ctgtcatcaa actgccattt gtgcacgcta nnnnnnnnnn
     7261 tcgtcgagac actgattatc acgtcatcgt tttttgccat tgctgctgtg ctggttttgt
     7321 ccgttctgct tattgaacgg acaggctagt tacgcagtgc ggcggaaatt gaccagttct
     7381 ggttgcgcaa tacgcaaata atccgcggtt ttcaaaataa cggatttatc cagcatgcct
     7441 gcattgaagg cgatttcatc aaatcgttcg aagagtaggg ggtcggcaac cagtttgagt
     7501 tttggatgga agctgaaagg ggggatcgcg ccgaagacac agcctgtgag ttcatcgact
     7561 tccgccgggc tggccaacg
//
LOCUS       DAWDRC010000125         7562 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4676_length_7562_cov_2.818436, whole genome shotgun sequence.
ACCESSION   DAWDRC010000125 DAWDRC010000000
VERSION     DAWDRC010000125.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4676_length_7562_cov_2.818436"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3036..3045
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3928..3937
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1778 a         1803 c         2025 g         1936 t
ORIGIN      
        1 aagtaattat gaggtaaatc tcatatattt attgggaaaa taaatacctt aaaggaaggt
       61 atgccgattt tgaatgacac cctttttcca tctcagaaaa agggcactat cttacttcac
      121 ccgcgccata taatacgcat cgacatattc accattacgc agcgcgtact tcttgccagt
      181 cccttcaatc tcaaagccaa attttttata gaccttaatt gctggcgcgt tatcgacgaa
      241 caccgttagc tcaatacgat ctacccgcaa ccagttgtcg cacatttcaa tcatctctcg
      301 catcagggcg ctggcgacgc cgcggttctt ccaacgggag tcgacacaga taccaaaatc
      361 ggcaacatga ctgcggcgtg ggcgttgttg cacgtcaatg gtgagatggc ctacgacgat
      421 tccatcaata caggcgacta actgcttgat gccagggcga gcggtgagtc gttccagcca
      481 catttgttct gaaggatgag gcacctgtag cgtgttgtaa tacacctccg gctgggcgtg
      541 aatctgcctg atggcctcgt gatcgcgtgt ttctgcgtgg cgtattacta tctcactcat
      601 tccttccttt gtcctctttg gggtaaatgt ccctttcaac atcattgact ttcaaatgcg
      661 agtcaaatgc atttttttgc aaaaagtgtt ggacaagtgc gaatgagaat gattattatt
      721 gtctcgcgat caggaagacc ctcgcggaga acctgaaagc acgacattgc tcacattgct
      781 tccagtatta cttagccagc cgggtgctgg cttttttttt gacctttcgt tctcaattta
      841 tcctcgggaa gtgcttgtgt tcgttatgcg cactccagta ggaaccacgt ccgctttgcg
      901 ctaaggtgta aataaccacc ttaaaaagga cgaaatcatg gtcattaact gcgcctttat
      961 tggcttcggc aaaagcacca cccgttacca tctgccgtat gtacttaacc gcaaggatag
     1021 ctggcatgtc gcgcatattt ttcgccgcca tgcgaagccg gaagaacagg cccccattta
     1081 ttcccatatc catttcacca gcgatctcga cgaagtgcta aacgatcccg atgttaagct
     1141 ggttgtcgtc tgcacccacg cggacagcca cttcgagtac gcgaagcgcg cgctggaagc
     1201 cgggaaaaat gtgctggtcg aaaaaccgtt cactccgaca attgcgcagg cgaaagagct
     1261 gtttgcactg gcgaaaagca aagggctgat cgttacgccg tatcagaatc gtcgctttga
     1321 ttcctgcttc ctgacagcga aaaaagcgat tgaaagcggc aagctgggag agattgtcga
     1381 agtggaaagc cattttgact attaccgccc ggtggcagaa accaaaccgg ggctgccgca
     1441 ggatggcgcg ttctatggcc ttggtgtgca tacgatggac cagattattt ctctgttcgg
     1501 tcgcccggat cacgtcgctt atgacatccg cagcctgcgt aataaagcca atccggacga
     1561 cacctttgaa gcgcagctgt tttatggcga tctaaaagcc atcgtcaaaa ccagccatct
     1621 ggtgaaaatc gattatccga aatttatcgt tcacggtaag aaaggttcgt ttattaaata
     1681 cggtatcgac cagcaggaaa ccagcctgaa ggctaatatt atgccgggcg aaccaggatt
     1741 cgcagcggat gattcggtcg gtgtgctgga gtatgtcaat gacgagggcg tgacggtaag
     1801 agaagagatg aagccagaga tgggcgatta tgggcgcgtt tatgatgcgt tgtatcaaac
     1861 catcaccaac ggtgcgccaa attacgtcaa ggaatctgaa gttcttacga atctggaaat
     1921 ccttgaacgc ggttttgagc aagcttctcc ctccgcagtg actctcgcga ggtaagtttg
     1981 atggcccctc gaatagttca atttttttga acagaggggt caattttcac cctctatcat
     2041 cccaggcgga tcgggtccac actaagccca tcgaaatcat tcagggggcg aatacaaatg
     2101 atctacttac gcaaagcaaa tgaacgcggt catgcaaatc atggctggct ggactcctgg
     2161 catactttct cttttgccaa ctattacgat ccgaatttta tgggcttctc tgcgctgcgc
     2221 gtgattaacg acgacgtgat tgaagcaggg cagggcttcg gcactcaccc gcataaagat
     2281 atggaaattt tgacctacgt gctggaaggt actgttgagc atcaggacag catgggcaat
     2341 aaagagcagg ttccggcggg cgagttccag attatgagtg cgggtacggg tattcgtcac
     2401 tcagagtaca acccaagcag caccgagcgt ctgcatctgt atcagatctg gatcatgcct
     2461 gaagaaaacg gcattacgcc gcgttatgaa cagcgtcgct tcgatgccgt gcagggcaaa
     2521 caactggtgc tttcgccgga tgcgcgtgat ggttcattga aagtgcatca ggatatggaa
     2581 ctgtatcgtt gggcgctggt gaaagatgag cagtcggtgc atcagattgc cgctgaacgc
     2641 cgcgtctgga tccaggtggt gaaaggcaat gtcaccatta acggcgtgaa agcctcgacc
     2701 agcgatggtc tggcaatctg ggatgagcag gcgatttcca tccatgctga tagcgacagt
     2761 gaaatcctgc tgttcgattt accgccagtc taaatttatc tcacaaacct tcctgaggaa
     2821 tcgggaaggt tctgatgcgt ccgtgttaaa ctaagagaat ctatctcttt tgtaccttca
     2881 ggacgatgaa aaagaaaaga cccgtacttc aggatgtggc tgaccgtgta ggcgtgacca
     2941 aaatgacggt cagccgtttt ttacgcaacc cggagcaggt ttccgtcgct ctacgcggca
     3001 agattgccgc cgctcttgat gaactgggtt atattnnnnn nnnnnattcc caatcgcgcg
     3061 cccgatatac tctctaacgc caccagccgg gcgattggcg tcctgttacc ttctctcacc
     3121 aaccaggttt tcgcggaagt attacgcgga atcgaaagcg tcaccgacgc gcacggttat
     3181 caaaccatgc tggcgcacta cggttataaa ccggaaatgg agcaagaacg cctcgaatca
     3241 atgctctcat ggaatatcga cggcctgatc ctcaccgaac gtacccacac gccgcgtacc
     3301 ttaaagatga ttgaagtggc ggggattccg gtggtggagt tgatggacag taagtcgccg
     3361 tgcctcgata tcgccgtcgg ttttgataac tttgaagcgg cacgccagat gaccaccgcc
     3421 attattgctc gcgggcatcg tcacattgcc tatctcggcg cacgcctcga cgaacgtact
     3481 atcatcaaac agaagggata cgaacaggcg atgctggatg caggcctggt gccgtatagc
     3541 gtgatggttg agcaatcttc ttcttactct tccggtattg aactgattcg ccaggcgcgg
     3601 cgggaatatc cgcagctgga tggcgtgttc tgtactaatg atgacctggc ggtcggcgcg
     3661 gcgtttgaat gtcagcgtct ggggttgaaa gttcctgacg atatggcgat tgccggtttc
     3721 catggtcatg acattggtca ggtgatggag ccacggctgg cgagtgtgct gacgccgcgt
     3781 gagcggatgg gcagcattgg cgctgagcgt ctgctggcgc gtattcgtgg cgaatctgtg
     3841 acaccgaaaa tgttagattt aggtttcacc ttgtcaccgg gcggatctat ttaagcctac
     3901 aaatttgaag tagctcacac ttatacannn nnnnnnntac acttaaggca cggatggata
     3961 ttgcttctga tattgtccgg ctggacaatg ttaccgataa cagttacccg taacattttt
     4021 aattcttgta ttgtgggggc accactttga gcacgactaa ccatgatcac cacatttacg
     4081 tcttgatggg cgtatcgggc agcggcaaat ctgcggtcgc cagtgaagtg gcgcatcaac
     4141 ttcatgccgc gtttcttgat ggcgatttcc tccatccgcg ctgcaatatc gaaaaaatgg
     4201 cgtctggcga accgctgaat gacgacgatc gcaaaccgtg gttgcaggcg ctgaacgacg
     4261 ccgcgtttgc tatgcagcgc accaataaag tatcgctgat cgtctgttct gcactgaaaa
     4321 aacactatcg cgacttgctg cgtgaaggta atcctaatct ctctttcatc tacctgaaag
     4381 gcgattttga tgtgattgaa agccgcctga aagcgcgcaa aggccatttc tttaaaaccc
     4441 aaatgctggt gacgcagttt gaaacgctgc aggagccggg tgcggacgaa accgatgtac
     4501 tggtggtgga tattgatcaa ccgctggaag gtgttgtggc aagcaccatt gaggttatta
     4561 aaaaaggcaa ataagtagtg actacattaa cgcttgtttt aacagcagta gggtctgttt
     4621 tactgctgct gtttttagtc atgaaggcgc gtatgcacgc tttcctggct ttaatggtgg
     4681 tgtccatggg ggctggcctt ttttctggta tgccgctcga taaaatcgca gcgacgatgg
     4741 aaaaagggat gggaggcacc ctcggcttcc tggcggtggt tgtcgccctg ggagccatgt
     4801 ttggtaagat cttacatgaa accggcgcgg tcgatcagat tgccgtcaaa atgctcaaat
     4861 ccttcggtca cagccgcgtg cattatgcta tcggccttgc ggggctggtt tgtgcgctac
     4921 cgctgttctt tgaagtggcg attgttctgc tgattagcgt tgctttctca atggcgcgcc
     4981 acaccggcac gaacctggtg aagctggtaa tcccattatt tgcaggcgtg gcggcagcgg
     5041 ctgcgttcct ggtgcctggg ccagcgccaa tgctgctggc atcgcagatg aacgccgact
     5101 ttggctggat gatcctgatt ggcctgtgcg cggcaattcc gggaatgatt attgccgggc
     5161 cgctgtgggg taatttcatc agccgctacg tggagttgca tattcctgac gacatcagcg
     5221 aaccgcatct cggcgaaggc aaaatgccgt ccttcggatt cagcctgtcg ctgatcctgt
     5281 tgccactggt gctggtgggg ctgaaaacca ttgccgcgcg ttttgtgcca gaaggctcta
     5341 ctgcttacga atggttcgag tttattggtc atccgtttac cgcgattctg gttgcttgcc
     5401 tggtggcgat ttacggtctg gcaatgcgtc agggtatgcc aaaagacaaa gtgatggaga
     5461 tttgcggtca cgcgctgcaa ccggcgggga tcattctgct ggtgattggt gcgggcggcg
     5521 tgttcaaaca ggtgctggtt gactctggtg tcggtccggc actgggcgaa gcgttaaccg
     5581 gcatgggcct gccgattgcc atcacctgct tcgtgctggc agctgcagtg cgcatcattc
     5641 agggttctgc taccgttgcc tgtttaacgg cggtgggatt ggtaatgccg gtcattgaac
     5701 aactgaacta ctccggtgcg caaatggcgg cgctgtcgat ttgtatcgcc ggtggttcga
     5761 ttgttgtcag ccacgttaac gacgcgggtt tctggttgtt cggtaaattt accggcgcga
     5821 ccgaagccga aacgcttaaa acctggacca tgatggaaac catcctcggc actgtcggtg
     5881 ccatcgttgg gatgattgcg ttccagctgt tgagttaatt actgcgacaa attactgcga
     5941 gcttgctcgc agtaattcat ttctcttttc tcctgtcttc atctttttga tctctctcgc
     6001 aaaaagaccg gaacattatc cgttctaatc actgtgttaa cggacgaggg agaagatgat
     6061 ggagaaacac ggagctgaat tgctgttaca gcggatgtta agtaacacca gcgctacttt
     6121 cagggaaggg caatgggaag ctatagatgc ggtggttaat cagcggcgaa aactactggt
     6181 tgtacagcgc accggctggg ggaaaagtgc cgtgtacttc atcgccagta aaatcttccg
     6241 cgatcgcggc gctggcccga caattatcat ctctcctttg cttgccctga tgcgtaacca
     6301 ggttgccgcc gctgaacggt taggtattac ggctgaaacg ttgaattcta caaacaggga
     6361 agaatggcag cgcattagcg ataagttgct gcaaggtggc gtagattgtc tgctgatttc
     6421 acctgaacgg ttggcgaatc aggattttct tgaaacggtt ttatatccag tcgctgaccg
     6481 catcggttta ctggtggtcg atgaggcgca ttgcatctct gattggggcc acgatttccg
     6541 cccggattac cgacgtatat tagatatttt gcgccaactg cctgcgaata cccctattct
     6601 ggggacaacc gcgacagcga ataaccgtgt cgttgaggat atccgtcagc aattgggtga
     6661 cattgtgatt cagcgtggaa cgctggctcg cgaaagtttg gcgcttgatg ccttagtctt
     6721 aggagaacag tcatcccgtc tggcatggtt agcaacggtt atccctcagt tttccaaatc
     6781 gggaattgtt tataccctga cgactcgcga tgctgaactt gtcgccgact ggttaaggaa
     6841 aaatggtatc agtgcatttg cttattacag cggcgtgact tgtgaaggtg cggaagattc
     6901 taatactgcc agggagtatc tggagcaggc actgctggca aataaaatca aagtgctggt
     6961 cgcgacgacg gcattaggta tgggatttga taaacctgat ttaggtttcg tcattcatta
     7021 tcagatgccg gggtctattg tcggttacta ccaacaggtg gggcgtgccg ggcgtgctat
     7081 agattccgca gttggcatat tgctttgtgg tggtgaagac cgcgctattc atcaattctt
     7141 ccgtgaaagc gcctttcctg cggaggcgca aattcatgaa atactcaacg tacttagcga
     7201 gaatgacggt cttaccctac gaggcattga acaacggacg aatcttcgtt atgggcaaat
     7261 agaaaaagca ctgaaattat tggtagctga aaatccatcg cctgtggtgt ataccgagaa
     7321 attatggcgc agaactatcg tcagtttttc tcctgatcat gaacgaatta accatttgat
     7381 gaatcagaga aaaagtgagt tggcagacgt tgaaagctat atcacgacca aggagtgcaa
     7441 aatgcaattt ctgcgccgcg cactcgatga gccaagtgcc gaacgttgtg gtaaatgtag
     7501 cagttgtctt cagcatccgc tgttgtcgcc cgacattgat agcggcctac tccatgaggc
     7561 aa
//
LOCUS       DAWDRC010000126         7517 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4707_length_7517_cov_1.982712, whole genome shotgun sequence.
ACCESSION   DAWDRC010000126 DAWDRC010000000
VERSION     DAWDRC010000126.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7517)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4707_length_7517_cov_1.982712"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1141..1150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2551..2560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3534..3543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4516..4525
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6903..6912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1662 a         2038 c         1899 g         1868 t
ORIGIN      
        1 gtgcggcatc tgttcccgga cgaataggga tccactcatc ttcacgcccg gcaccggtgt
       61 cggtatagcg cggatcgatg atgatcatgc gagcattaga tttctggcgc gcctgttcaa
      121 gatagtaagt caccccaccg ccactcattc gcgtttcgcc agggttatta ccaaacagca
      181 ctaccagttt actgttttcg atatccgacg ggctgttacc atctgcccag ccgccgtagg
      241 tatagttcag accttccgca atttgcgcgg aggagtagtc gccataatgg ttcagataac
      301 cgccgcagca gttcattagc cgcgcgacca gggtatttcc cggcggccac gagcgggtca
      361 tggtgccgcc cagcgtaccg gtgccatagt tcagatagat agactcgttg ccgtactctt
      421 tgatcaggcg ctgcattttg gtcgcgatga tgtcgtaggc ttcttcccag ctaatgcgct
      481 cgaatttgcc ttcaccgcgc gccccgactc gtttcatcgg atatttcagg cggtccggat
      541 tgtagacacg gcgacgcatg gaacgcccac gcagacaggc gcgaacctgg tgcaggccgt
      601 cgtaattgtc atcgccggta ttgtccgttt cgacatattt gatttcaccg tccactacgt
      661 gcatacgtag cgggcagcga ctaccacagt taactgtaca ggcgctccag ataacctttt
      721 cgtctgattt tgttggaata gcgctatcga cagcgtgcgc aatccgacta aaaggtaatg
      781 ttaatgcgct gctggccatt gccaggccgc cgatcgctgt cgtttttacc aaaccacggc
      841 gactcacctc agcagccaat accgcatcag ggattttcgt tttcataatg gctcactcaa
      901 gcttgctcac gattgattcc agtaacaaac cgtattgtgt aattatttct atagcagatc
      961 gctatatagg cttgtataca tcgaataatt tgaaaatcaa agagataggt agattttcac
     1021 tcgaaagtag tattacttgt aagtgaggag taattattgt tcggtatcaa agggagacaa
     1081 aaaaagcgcc cgcaggcgct ttttagattc agaaaaattg ggtattagcc aatatattcc
     1141 nnnnnnnnnn atatattcca gtccgttcat atacggacgc agaacttctg gtacttcaat
     1201 acgaccatca gcctgctgat agttttccat tactgcaacc agcgtacgac caacagcaag
     1261 accagaaccg ttcagggtat gaaccagacg ggttttcttg tccgacttgc tacggcaacg
     1321 tgcctgcata cgacgtgcct ggaaatccca aacgttggag caggaagaga tctcgcggta
     1381 ggtgttctgt gccgggatcc atacttccag gtcgtaagtt ttgcaagcgc caaagcccat
     1441 gtcgccggta caaaggatga ttttacggta cggcaggccc agcagctgca ggactttttc
     1501 cgcatgaccg gtcatctctt ccagcgccgc cattgagtct tccgggcgca cgatctgcac
     1561 catttcaact ttgtcgaact ggtgcatacg gatcagacca cgagtgtcac gaccatatga
     1621 accagcttca gaacggaaac atggggtgtg ggcggtcatc ttaattggca gatcatcttc
     1681 atcgatgatt tcaccgcgta ccaggttggt cagcggaact tctgccgttg ggatcagcgc
     1741 atagttactg gtgtctgctt cttcttccag cggacgagta tggaacagat cgccagcaaa
     1801 tttcggcagc tgacccgtac cgtacagcgt gtcctggtta accaggtacg gaacatagtt
     1861 ctcactgtag ccatgctgtt cggtatgcag atccagcata aactgcgaca gtgcgcggtg
     1921 catgcgagca atctgccctt tcattaccac aaagcgggaa ccagtcaact taactgcggc
     1981 tgcaaagtcg aggccagagt gcatttcacc cagcgtcaca tggtcacgaa cttcaaagtc
     2041 aaactcacgc ggggtgcccc agcgactgac ttcaacgttg tcattttcgt ctttacctac
     2101 cggcacttca tctgcaggca ggttagggat ggttagcgcg atatcgcgaa tttcagcctg
     2161 taaagcatcc agctcggctt ttgctgcatc cagctcttcg cccagtttgt tcacttccag
     2221 acgtaaaggc tcgatatctt ccccgcgcgc tttcgcctgg ccaatggatt tcgatcggga
     2281 gttacgctcc gcttgcaggt tttccgtttt gacctgcaat actttacgac gctcctcaag
     2341 agcgcccagc ttatctacat ccagcttaaa gccccggcgt gccagttttt cagcgactgc
     2401 gtctggctca ttacgcagca gattgggatc gagcatgctt atcctgtgct tatcgaatta
     2461 aatgggagat gtggccacag cctgcgacca cagggataca ttaacaacat taccgcaacg
     2521 ataacattaa cggtagcgtt ttatggggct nnnnnnnnnn ggggctattt tgatcctgtt
     2581 cagccagcca ggcgagcttt tcgccaatct tgccttcaag gcccctgttt gtcgggaaat
     2641 aatagcgtgt ttgtgctatt tccggcggga agtaaacctc accggcagca taagcgtttg
     2701 cttcatcatg agcgtaacga tattcctgcc cgtagcccat ttccttcatt aatttcgtcg
     2761 gcgcattacg caaatgaacc ggcacgtcat aatccggacg ttcgcgagca tcggccagcg
     2821 cggctttaaa cgcagtgtag acagcgttgc tttttggcgc acaggccagg taaacaatcg
     2881 cctgagcaat ggcgcgttca ccttccgccg ggccaacgcg agtaaagcaa tcccaggccg
     2941 caattgccac ctgcatcgcc cgtgggtcgg cattgccgac atcttcggaa gcaattgcca
     3001 ggcaacgacg cgcgacatat aacggatcgc caccggcggt aataattcgc gcataccagt
     3061 agagcgccgc atcgggtgcg ctaccacgta ccgacttatg cagtgcggaa atcagatcgt
     3121 aaaagcgatc gcctttgtta tcaaagcggg cgctacgttc accggcgatt tcggtcagta
     3181 attcaggctt caggacccgc ttaccgctat catcgacttc ggccatatcc gccatcattt
     3241 ccagcgtatt taacgcccgg cgcgcgtcgc cattcaccag ttcagcaatg gcgcgtcgtg
     3301 tttcatctgg cagaacaata tcctgaccac catagccacg ggttttgtct tccatcgcct
     3361 gagttagtac ttgctcaata tcctctgtac tcagggattt caacagatag acacgggcac
     3421 gggaaagcag tgccgaatta agctcaaacg acgggttttc agtggttgcg ccaataaaag
     3481 tgatggtgcc gtcttcaata tattggtcga gaggaaccac caaaccgtcg tttnnnnnnn
     3541 nnncggtttt gccgagcgcg ctcgatcgcc tcgcgaatct ctttcacacc agaggtgacg
     3601 gcagaaatac gttccacatc agcgttcgca tagcgggcaa tcacttcagc gagggttgtt
     3661 ttgccggtac ccggcggccc ccagaggatc atagaatgca gatgcccggc ttcgatagcg
     3721 cgcggcaacg gcttccccgc agccagcaaa tgttgctggc cgatatactg tgctaaattt
     3781 tctggccgca tacgcgcggc cagaggttga aaagtattat ccgaaaaatc gagcgacaga
     3841 ttgctcactc aggtgcctct acttacgttg atcatctacc gtgacgcctt gcggcggggt
     3901 gaaggtaaat ttcgctgcat ccacagcccc attttgctgg gatttcagct gataactgct
     3961 gcgctgatcg tcctgctcca ccgcgctaaa ctgatggatt gtgccatcac gtcccacgtt
     4021 aatggtgaac tgcttcagat tgccattgct ggctttcggc gtcagaacaa agtcatcgcc
     4081 attctgtttg atattgtact gctgccagtc gctggactgg ttgcgggcaa tcagcataaa
     4141 cggcgtatta ccggtggcat ctttcagcca ggttgccgta gcttgctcaa cgaacgggtt
     4201 atagaaccac agtgttttac cgtcagaaac cagaatgctt tcatcaggtt gagtcatatg
     4261 ccagttgaat aagtttggac gtttcaccca cagatcgccc tgaccttcct gcaccgccgc
     4321 gccgctaccg tcagtcactt tctgtgtgaa gctggcgtgg aagctgctga ctttatccag
     4381 gcggctttta agatcgcttg cggcatcagc ccaaacgctg cttgctacta agcttgagag
     4441 taatgcacag gtgatggcaa tttttttcat cattattcct caaattacgt cactcccaat
     4501 cgggagttcc ctctannnnn nnnnncagct tgaggaaaga agaaaatact gaattttcag
     4561 ttgatagaat accggatgcg acgctatcgc gtcttatccg gtctacgttg cattaattag
     4621 tcaaatggcg gtggggccag cacttcacga ttaccgttgt gcccctgttc gctgacaatc
     4681 ccctgcgctt ccatctgttc gataatacgc gctgcacggt tataaccaat gcggaactga
     4741 cgctgtacgc cagaaattga cgctttgcgt ttttcagtga caaactgcac cgcctgatcg
     4801 aacaacggat ccagttcttc agcgccatcg aaaccgccca cgccaccttc gctttcactg
     4861 tcggaggtga tgccatcaac atactgtggg cgaccacgcg ctttccagtc ctgcaccacg
     4921 gcatgaactt cctgatcgcg aacaaaagca ccatggacac gtaccggcaa cgtggagttc
     4981 ggcccagagt agagcatatc ccccataccc agcagtgatt ccgcaccagc ctgatcaaga
     5041 atggtacgtg agtcaatctt actggatacg gtaaaggcga tacgggtcgg aatattcgct
     5101 ttaatcagac cagtaataac atcaaccgat ggacgctgcg ttgccagtac gaggtggata
     5161 cccgcggcac gggctttttg cgccagacgt gctatcagct cttccacttt tttacctacc
     5221 gtcatcatca ggtcggcaaa ttcgtccacc aacaccacaa tgtatggttc ttttttcagc
     5281 accggatgct gcgcatccat actatcaccc ggcttccagt acgggtctgg aatcggacgc
     5341 atcatgcgat cggcttcagc aattttttcg ttataacccg ccagattacg cacacccagc
     5401 gcagacatca gtttataacg acgctccatc tcgttaacac accagcgcag cgcgttggcg
     5461 gcatctttca tatcagtaac aacttccgtt aacaggtgcg gaatgccttc ataaaccgaa
     5521 agctccagca ttttcgggtc gatcatgatg aaacgtacat cttctggctg tgctttataa
     5581 agcatgctca ggatcatcgc attcaccccg acagacttac cggaaccggt agtccccgca
     5641 accaacaagt gcggcatttt cgccagatcg gcaaccaccg gctcaccggc gatatcttta
     5701 cccagcacca cggttaatgg cgacggatta tcgcggaatt tggcgttatc caaaacttcg
     5761 cgcagataaa cggtttgtcg ttttttattc ggtaactcca gacctacata gggtttgcca
     5821 ggaataactt caacgacacg caccgccacc gtcgaaagtg aacgggcaag gtcacgtgac
     5881 aagttagaaa tgcgcgctgc ttttacgccc ggtgccaggt tcaattcaaa gcgagtgata
     5941 actggccccg gagagtaatt gacaacatcg gctttaatac ggaaatcagc cagacgcgct
     6001 tctaccaggc gcgccatttg ttcaagcgca aaggtatcta ccggctccac ttcgctcggc
     6061 ggcggtgtca gcaaatccag agaaggcagt ggcgtcgtcg gtttatgcaa cggacggctg
     6121 tcgccattac gcatcaacag cggatgaagc agggtatcct gcggctgtgg tacaaccggc
     6181 tgttgcggct gctgatactg cggctgctgt gtaaccggct gttgtggctg ctgatattgc
     6241 tgctgcggtg caaccggttg ttgcggctgt tgatattgcg gctgtggcgc aactggttgt
     6301 tgcggctgct gatactgcgg ctgcggtgca accggttgtt gcggctgctg tacaggttca
     6361 acaattggcg taaacagcgg ttcgtgtgga ccatcatcca gcaacgcttt cattggcgaa
     6421 aattcaaaat catccagcga gaacggattc gccccagccg gttgttcgcc ggaataacgt
     6481 tgttgttgag tttgagcaaa ctgacgagcc agttctgcct ctgccgcagc atctgcatct
     6541 tctgtgttca cgggcacatc atgttgatac tgttcgccat agcgttgctg ctgcgtctgg
     6601 gcgaactgac gtgccagttc atcctgctgc atcgcatcga tttcatcatc gttgtactga
     6661 tcgccagaat cgtactgatt acgctgggct tcgcgggctt tttcttctgc cgcacgctgt
     6721 gagggcagct taataccgta agacgccagt tcacgacgag ttggcacgcg gatgcgtttc
     6781 ggtcgtggca actgcgggcc aatcccctct ttgacctgcg gacgcggtcc accgctattt
     6841 gccagactga agactggcgc ggcaacggtt gcggcagccc ccgtcgccag tgtcgcttct
     6901 ttnnnnnnnn nncagcggct tctactggag gtactgctgc aacagaaggt gctttcagcg
     6961 aagatttgat cggttccggt tctttaaccg gttctggaat cggttgatac caggccgcaa
     7021 gttgttcacg ttcacgggct cgcttctctt ccacttcttc aaaatagtaa agcggcggac
     7081 gcgtgggttt tgtctcttct acaacgggtt caggctccac aacaggctgc tgttcaacgg
     7141 gttgcggctg ttggtacaac ggctcctgag cagctggctg ctgataagtt tgctccgtct
     7201 ggtatgtaga ctgtggagca aaagtggatt gctgctcttc ggcttgccag gcgttacctg
     7261 ccaccggttg ttctggcgca ggggcgtaat acggttgctg aacaggttgt tccgccgcag
     7321 gagcgtagta aggttgttgc acaggttgtt cagccgcagg cgcataatac ggctgctgtg
     7381 gctgtacagg ttgttgcagc ggctcattat attgcactgc aggctgcgca tattgtgact
     7441 gctgtgggta accttccggt gcaggtgcaa taaccggttc tcccgtttgc ggccccggta
     7501 caggctgcca ggctact
//
LOCUS       DAWDRC010000127         7503 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4722_length_7503_cov_2.645677, whole genome shotgun sequence.
ACCESSION   DAWDRC010000127 DAWDRC010000000
VERSION     DAWDRC010000127.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4722_length_7503_cov_2.645677"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    536..545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1786..1795
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3598..3607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1727 a         1988 c         1943 g         1815 t
ORIGIN      
        1 tttacgtgcc agcgccattt tttcgcgttt ctctacactt tccgcagtct cttcatacag
       61 caaggctgcc tcgctggcgg ggcgacgctg ttcagtaatc gcctttacaa tcaccgtgcg
      121 ttcgtcattt ccctggcgca gagtgagcgt ggcattcagc tcgacgattt tgcttggctt
      181 gctgcgctgc ccgttgtaat gcaccttacc gccttcaatc atttcacggg ccagcgcgcg
      241 ggttttataa aaacgggcag cccatagcca tttatccagt cgaacctcaa cagcaggttt
      301 ttctttcatg gcatctcctt cacattagcg aggggatcag gcggcggtag tcattcagtg
      361 acggatggcg ttgatactgt ttctcggcaa tcccggagtc aggattagtc acaccgaggc
      421 agtaacgaat accaaattgc gcggcagcat cgagaatcgc ttcgctgtca tcaataaaca
      481 gcgttctttc agctttaaga cccgtagctt cggccaccgc atgccataac cgctgnnnnn
      541 nnnnngctga tcctctttcg gataaccaaa tgtgtgggtg gaaagtaata aatcaaggtg
      601 tgcgtccaga ccggtatgct caagttttac cgccaggttg tgcggatgcg cattggtgag
      661 caaaattcgc tgtttaccgc tggctttcag tgcctcaaga aacggaatgg tatcttcacg
      721 cagtacggca cgcggtccca tctcggtggt catcgcacag atatccagac ccagttgctc
      781 actccagtaa tcaagacagt accagtttag cgtatgctgt acgtcgtgat attgctggcg
      841 catatattcc atcgcttcct gtggcgtaac tccgtttttc gcgccccatg tttcaggcac
      901 cagcttttgc cagaaatagt tatcgaaggc gaggtcaagc aacgtgccgt ccatatccag
      961 cagaacggta tctacgtcct gccaggcaat gttgatatgc atgagggaaa tctccagagt
     1021 gaagcaattt gcgcgacagg gtagcataac ctgccgcgca aacgtgttat tcgataaggc
     1081 tttctgaagg ggtgatcagt tgcgggttca ggcagctttc ataataggcc tgaatcttca
     1141 tcatgcgagt acgatggcgc tggtaacgac gccaggcctg tacgccatta taaatggcgc
     1201 tacccagcat cgtcagtagc agcagcgtgg tgctgagata gcgccacagg ccggaacgat
     1261 ccgggatcgg atgaaggcca atatgctgcg taccattggc gtcagtgaag atttttgtga
     1321 cgataccttc ggcgttaaac ggcgtatgca tcagcatttg tgccagtttc tggaaagcgt
     1381 tccactgttc ttgcggcgga tagtcgtaaa gtgatgccga aggccagggc tgatcaacaa
     1441 aatcgctgcc ttcgtcgctg acaatcagga atccgccggg ggccggactg tttagtgatt
     1501 gtgccgctcg cgccgtttca tgcgtgataa acggcgcggt agaggtggcc accaggttat
     1561 ccagcgattc cgcactcacc gggcgtaata acacattcac gccatcgagt ttcccggcgt
     1621 tggcgcgttt taccagcgcg tcccagtctt tactgttgcc gagattgacc agcgcatttt
     1681 tcagtcgcac acagtcatct ttggcagaac acagatctgc tgtcttcaga acaatgtcgc
     1741 caaaatcatc aagcaatacc ataccggatt tttgaattgc tgaacnnnnn nnnnngaggc
     1801 actgacgcga gattcatctt ccggtttagg gtgcagctgg cgattaactg cttcggtcaa
     1861 tgccgtcgct ttgttgacca attcagattc tggtaatggc aatgagcggg cgtcattcca
     1921 gatgatctgc gagcagtcaa acggtaaaaa aggtgagttg gttttcgcgc tccaggtacc
     1981 ggaagtgcga atattacaca ttcccgtacc gctaatacgc aatgtatcgc caacccgcac
     2041 gccagcgtca gccagttgtt tcacgctggt ggcttcaatg gtctgcgcgc ctttcatcca
     2101 cgagagggtg aatttcagcg gcatatccag cgggatccag aataacagca taaacagcac
     2161 caatagcgag cccgcagcaa taatcgtact gcgcagccag tgctgtaacg gaaagttttt
     2221 tacttcatca tgcagcgaaa gatatcgccc ctgacgcact acgtggcggt cgagatagat
     2281 atcgatatcg gtttgttgac caagatcctg agcaatgtat ggctgccagt gtgcgggata
     2341 gaccaggtcg ataataccga gtgaaatatt gttgatctgt tcctgatcgt tttcgccaaa
     2401 caatccccaa cgacggggtg taccgcgcag acaatgaatt tcccgcaggg aggattttgc
     2461 cgggggcgcg aatagccccc acagacctgc gcccagcagc agtaacgcgc cgcctgccag
     2521 ccacggaaca aagacatccg gggtaatcag gcagaaaaag aacatcatga aggaggcgac
     2581 aatcagcaac gcttcgcgca gcccgcgcgg acgactcagc gcatactctt cgtgcgtttc
     2641 tttgcgaata ttgagcagct ctatttgctc gctctcttcc ccgcgaatcg acgcctgcgt
     2701 tgacggaaca ggttgcaagg catagccgcg agtttcctgc atgtactcct gcagcgtatg
     2761 accgttgagt gaaataacca gcggcagcga atcggtatga atcagttcaa cggtattctc
     2821 atcgttgatg tactgttccc agaaaggggg caggtggact tctaccgaat cgaggtagta
     2881 acgccattta ttcggatcgt cggtagagat gccataacgc gtgatagcgt gtgtcagcat
     2941 catgacgttg ttgctttcgg cattcagcgc cagagagatc ggtgccgcgc tggctcccgt
     3001 tgggccaggc acctgtaata cctgcgtcag gctctcaaga taattttcaa cggcgctacg
     3061 ttcttcagat gtgagtttac gcgtttgcgc atccgcgaag gcgttggtcc agggcagctg
     3121 acgccgtctg gatcgcactt ttatcagcca tcccgcaagt agtgagcagg ccagcaaagc
     3181 agctaaaaaa atcacaatgg tgctcatgct ttccccatct tacttagtct gtcaggcgtg
     3241 gtcagtcgca ccggacacaa tacatgattc tgcggttggc tatcggcaag cgtggagttt
     3301 aacttgagca tcattaagca catcacagta accgctgatc atagcaaacc ctctgaaggc
     3361 ggaatatcag caaattcctg gagttatttc aacctattga cttttaattt gatttgagga
     3421 cgattattta ttaagttaca taaatgtaaa taacacgcaa ttcacattgc cgaaagcgtg
     3481 tccgatatcg cacaataacc gctgatttca cagcatagat cccacgatga gcaaatcatt
     3541 acaaaaaccc accattctga atgttgaaac tgtagcccgt tcccgactgt ttaccgtnnn
     3601 nnnnnnnaaa gcgtggatct ggagttcagc aatggcgtgc ggcgtgttta tgaacgaatg
     3661 cgtccaacca accgggaagc agtgatgatt gtgccgattg tggacgatca cctgatcctg
     3721 attcgcgaat acgcagtggg aactgaatcc tacgaattag gtttttcgaa aggattaatt
     3781 gatccgggtg aaagcgtctt tgaagctgct aaccgcgagc taaaagaaga ggttggattt
     3841 ggtgcgaacg atctgacttt tttgaagaag ctcagcatgg caccgtctta cttttccagc
     3901 aaaatgaata tcgtggtagc gcaagatctc tacccggaat cactggaagg cgatgagcca
     3961 gagccgttac cacaggtgcg ctggccgctg gcgcatatga tggatttgct ggaagcccct
     4021 gacttcaacg aagcgcgtaa tgtcagcgcg ctgttcctcg tgcgcgaatg gttgaaaggg
     4081 caggggcgag tgtaaatgcg tgaagtttgc ctgatgcaca acgcttatca ggcttacgaa
     4141 gttcctgcaa tttattgaat tttcaatttt ttgttggccg gataacgcgt tcacccgcat
     4201 ccggcataaa caagtgcact ttgtcagcaa actgaaaaag gcgccgaagc gcctttttaa
     4261 tcagaacaat tcctgtgcct cgccattatc gataatggtc gttcccacct cgtgcaccgc
     4321 ctgttgtgtc ggctgcgtac cttcgatgaa atactcttcg cggctgttgc caccattagc
     4381 taattgcccg gtgctgcgat cgatattcac cgtcacaata cccggtggcg gcgtcagcgg
     4441 ctgttccggc acaccttcaa gaacggcttt catataagcg tcccatgcag gctgggcact
     4501 cttggcaccg ccttcgtaac ctgagatctg atctttaatc gctccggaag ccgttgtatg
     4561 accgagatta cgacggtgat catcaaagcc aatccagacc gaggtcacaa cgcccggacc
     4621 gtaacccgag aaccacgcat ctttcgaact gttagtggta cccgtttttc cgcctatatc
     4681 atgccgttgt aaatcacgac ctgctcgcca gccagtacct tgccagcctg gttcaccaaa
     4741 aatattggtg ttcaaggcac tcttaatcag gaatgccagc ggtgtgttga tgacgtgcgg
     4801 tgcgtactcc tgcgcgccag tcttcgccac taacgcctga tttgcctgct ccagctgcgg
     4861 cattggtaca gaaacattct gctgctcgcg ggagatagcg acatcttcaa catcgttatt
     4921 ttccagcacg ttcgatttct gcgtatcacc gtaaatcacc ggaatatcgc attccgggca
     4981 ggctactttc ggtttcgctt cgaaaatcac gccgccctga tcgttttcaa ttttgctgat
     5041 aaaccacggg tccaccagga agccgccgtt cgccatgacc gcgtagccgc gcgccacctg
     5101 cattggggtg aaggacgctg aacccagcgc cagcgattcg gtgtggacaa tgttttgtgc
     5161 cgggaagccg aagcgttgca gatattctgc agcgtagtcg acgcccatcg cccgcattgc
     5221 gcgtaccatc accacgtttt tcgactgacc cagcccctga cgtaagcgaa ttggaccggc
     5281 atattgcggt ggtgagttct tcggctgcca gtcagaaccg gcacctgcat cccagcggga
     5341 aattggcaca tcgttcaaca tacttgccag cgtcagacct ttatccatcg ccgcggtgta
     5401 gaggaacggt ttgatgttgg aacccacctg acgcagtgcc tgagtggctc ggttaaactt
     5461 gctctgattg aaatcaaagc caccgaccag cgccataacg gcaccgtttt gcggattgat
     5521 cgacaccagc gccgagttca cttccggcac ttgtgccagc caccatgcat cgccaacttg
     5581 acgaacccag atttgctgac ccgtttgcag aacatcggtc actttacgcg gcgtcggtcc
     5641 ctgctgagta tccgaacggt aaggacgcgc ccagcgaaca ccttccatac tcaatacgac
     5701 ggtcgacccg tctgccagca tcgccgttgc ttcctgagga ttggcgctgg tgactgccgc
     5761 aggcagcagc ggaccatagg tcggcagcgc cttcagcgta tcggtaattt tgttgttgtc
     5821 ccacgccgat tcgcccactt tccacagcac attcgccggg ccgcgatagc cgtggcgcat
     5881 gtcgtagtcc agcacgttat tacgtaccgc ctgctgtgcg gcctgctgca ctttgtgggt
     5941 gatggtggtg taaatgcgat aaccgtcttc ataggcactt tcgccataac ggttatacat
     6001 ctcctggcgc accatttcgc tcaggtatgg cgcagagaaa gcaatctccg gcgcgtgata
     6061 gttagcgtta atcgcctcag tgcgtgtctg atcgaactgt tgttgggtga tatacccttc
     6121 atccagcatc cgcgacagca cgacgttacg ccgcgcgacg gcacgatcca ttgagtagag
     6181 cgggttgaag gtggaaggcg ctttcggcag cccggctatc accgccattt cgttcaacgt
     6241 cagttggtcg accgttttac cgaaatagac ttgtgccgca gcaccgacac cataggcgcg
     6301 gtaaccgagg taaatcttgt tcagataaag ctcgaggatc tcgtctttcg tcagcaactg
     6361 ttcaatgcga atcgcgagga agacttcctt aatcttgcgc atcagcgtgc gttctggact
     6421 gaggaagaag tttctcgcca gctgctgggt aatggtactc gccccttgtg acgcgtgacc
     6481 ggagaacagc gccacgcttg ccgcacggaa gatccccacc gggtcaacgc cgtgatgctc
     6541 gtagaagcgg ctgtcttccg tcgcgataaa ggctttcacc atctccggcg ggatttgatc
     6601 caacgtaacc ggaatacgac gtttctcacc gtattgagca atcagctcgc catcggcgct
     6661 gtaaatctgc atcggaattt gcaggcgaac atcttttaat gtcgccacat ccggcagttg
     6721 tggctcgatg tagcggtata ggccataaat cgagcctgct cccagcagaa tgcaacagac
     6781 tgcaaggatc aaaaaatact ttacgaactt cactggaaat ttcccattta gtttcatttg
     6841 ggcagtttat aaacaaacgc gcggtagtat aaaggcaagc cagacgcatt gatatacccg
     6901 tcagagtgac gggtgataag gagatcatca caatggcatt taagaactgg caaattggtt
     6961 tgcatttaca acagcaagaa gcggtagcgg ttgctatcgt gcggagcgca aaagaatgct
     7021 tattgcaccg ctggtggcgg ttgccactgg agaacgacat tatcaaagat gggcggattg
     7081 ttgatgtgca gcggctggct aacacgttgt taccgtggag tcgtgaactg ccgcagcgtc
     7141 atcacattat gttggcgttt cccgccagtc gcacattaca gcggtcattt ccacgcccgt
     7201 cgatgtccct cggtgagcgg gagcaaatgg cctggctgtc cgggacgatg gcccgtgaac
     7261 tggatatgga ttcggactcc ctgcgcttcg attacagcga agactcactc agtcccgcct
     7321 ataacgtgac tgccgcgcaa agcaaagagc tggcaacgct gcttactctg gcagaaaggt
     7381 tgcgtgttca tgttagcgcg atcactccgg atgccagtgc attacagcga ttcctgcctt
     7441 ttttaccttc tcatcagcaa tgtctggcct ggcgtgataa cgaacagtgg ctgtgggcga
     7501 cgc
//
LOCUS       DAWDRC010000128         7484 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4739_length_7484_cov_2.604119, whole genome shotgun sequence.
ACCESSION   DAWDRC010000128 DAWDRC010000000
VERSION     DAWDRC010000128.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4739_length_7484_cov_2.604119"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6941..6950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1823 a         1896 c         1986 g         1769 t
ORIGIN      
        1 cctgtatcgc tttataaatc aggaacagcc cgcccccgag cagaatcaag gtgcggaacg
       61 aaatctccag cgtcagcacg gtaaaaagcg gctgggtaat atgtgaaagc cacgcaatgg
      121 atatcagcaa taatatccgc ataatcatcg cgcagcttaa acccagttta cgcgccatat
      181 tttgttgtga taacggaagt tttgccacca ccagtgaaag aaaaataata ttatccaccc
      241 caagaataat ttccagaaag gtgagggtaa ttaaagtgag ccagatatct ggctgcataa
      301 accacaaaaa catggggtaa tcctttattc tctttatcta aactttatag cgggctggat
      361 aatagagtta atttaattaa tgttataatg atgaatgatt tgttgaatct cgctgatctg
      421 ttcaggcgtg aaattaaccg cgtagcggct atcaccaaca tcatgcccca taagctgcaa
      481 cgcttttttc accgcagccg gggagaatgc tactttataa agatcggtac gcaggcccgt
      541 tacactctcc tgtgcttcat aagacccttc aatatcaccg gcatggaagc gagaccagat
      601 ggcattgatt tctcgcggcg cgatgttggc gagcccagag atacaggctg aacatccctc
      661 gacaaaccct ttatggatca gcgaatccgg gccgttgagc acattaaagc cgtcaatgtc
      721 gcgcaccgca tcgagaaaac ccttcaggct ttcgtcgctt ccggcgctgt ctttaatccc
      781 aatgatattg gggtgcgacg ccagagcacg cgcggtttct ggctggatcg tattgccagt
      841 gcgggccggg atgttataga gaaacatcgg aatgctcagt gcgtcggcaa ttgcgctgta
      901 atgcgcaatc aactcttcct gtttcagcgg tacgaagtaa ggggcaatga ccgacacggc
      961 atctacgcca agtttttcaa tctgctgacc cagacgaatc gtttcgcgcg tagagatctc
     1021 gccaatgtgt gccacgaccg gcgcacgacc agccacttca tccacgcagg tttgcgtaac
     1081 gctgagcttc tcctgttcat tcaacacaaa aaattcgccg ttggtgccgc cgcaaaaaat
     1141 cgcgttaccc gcttccagtt gacgattcac ctgcactttt aacgccggga tattcagact
     1201 gccggtcgcg gtaaacgggg tgacgatagc ggttaataca ccttcaatgg ttttcggcat
     1261 gatttattcc ttaaattgat gttgcggaaa cagtgtttgg taaatggcct gtacgtcctg
     1321 tagatcaagc tggcacggca cattgttcat aaggcgcttc acgttcagtg ccgcctcgct
     1381 cagagaggca atatcctctg gcggtacgcc gagatccgcg agattgttgg gtagcttgag
     1441 ttgatggact aatgcctgta accaggtcac cagggcatga gatttttctt ccgtgctcag
     1501 ggcggtttcc gcatcgggaa tgcaatccca gacccgggca aatttctcga ccgcccaggg
     1561 gcgaacaaac gccatgcacg gcgccagcaa gatggcattc gcgacgccat gcggcagatg
     1621 atatttgcca cctaacgggt aggagagcgc atgaacgaga tgcgtgcccg catgggttat
     1681 cgctacgccg ccatagtaag acgcccacag catttccagt tttgcgcgca gatcctgagg
     1741 atcgttcacg gcgggttgaa tgtgccggaa aagtttactt aacccagtca gcgccgcgtt
     1801 atcgctgacc ggatttgcca cggtcgcggt aaaacactcc agtaagtggc aaagagcatc
     1861 aatgcccgtg gacgccgcaa tatgcgcggg catgctggtg gtcagttccg ggaaaagcgc
     1921 cacatagtct ggtaacagca cctgggaaat aatacccacc ttcgtgcttt gctcagggat
     1981 tgccagaatc gcattcggcg tggcttctga gccggttccg gcggttgtag gaatcaacca
     2041 tgattgcacc cgctgagtcg gtttttcacc cgcaagcagc gcatccagcc ccggtgattg
     2101 tggatggcaa agcaccgata gcagctttgc cacatccaac acgctgccac cgccgatccc
     2161 gaccaccaca tcaaaagagg catcgccaag gacatccatt agctggcgca catcatgatg
     2221 cgtgggctct gcgggcacat tatcgataac gttagcctgg gggccgggtt tttcccgtaa
     2281 ggcgcgaatc tgctgcacac cgtcgagctg cgcaatatta cggtcagtga ccagcagcat
     2341 ttttctgatg tcgggtaaga gcgacgtcag ggaagggata gcgcctgcgc cgctcagtat
     2401 cgtgctattg ataattacca caaataacct ctcttctggt gattgaattc aatcattcaa
     2461 aatgccgtag cctacgtaac atggtctaat tttataattt attcgagtga ttaataactt
     2521 gagcttgctc acatttaatc aatcatgatt gaatataatc attgttatca taaaacaacc
     2581 tggagtgatg atggaaaagg gaatcactaa gccgagcatt ctcgtcgttg ccgatgattt
     2641 cacgggagcc aacgacgcgg gggtaagcct ggcgcaggtg gggcatacag ttgatgtggc
     2701 ctttgaaatg ccttatcgcg gtgatgccag cgtatgggtg attaacagcg acagtcgggc
     2761 gatggatcct aagctggcgg cgatgaaaat aacctcgctg atgagtcatc ttcctctggc
     2821 gaataacccg ccgctcgtga ttaaaaagat agattccacg ctgcgaggca atatcggcgc
     2881 agaaattgaa gccctgatga aggcatgcgg tataacgggc gcagtgattg cccccgcatt
     2941 tcctcaggtg ggaaggacca cagtggccgg tgaatgttgg gtaaacggtg tgcggataac
     3001 ggaaaccgag tttgccagcg atccgaaaac cccggtattg agtgcccgaa ttgcggacat
     3061 catccggttg cagacggtca ttacatgcca gcccgttacg gtttctcagc tcagccattt
     3121 gtcttatgag caaccgtgga ttggcgtgat agatgcgcag acggacagcg acctggatcg
     3181 catcgctgcc gctgtgatgc aggctaaaca accgctgctg ctggtcggct ccgctggtat
     3241 ttgtgatgcc gttgcccggc gctccacgat tatgccgcca cccaccgtgc tggccattat
     3301 tggttcaatg agcgaaatcg ctcaacggca gatcgccaca cttcattcac acccacgtat
     3361 cacacagata tacgtcgatg ttgaacacat tctggcaggc aatgccagtg actatgacgc
     3421 gcgtattgtg caggccttgc agaaaggcga ccactgcatt gtccacacct gtaacgattt
     3481 tgtagcaagg catcagatcg acaccctttg ccagcgttgg cagatgagtc gcgctgcgct
     3541 tggggaaaaa atttgccggt ttcttggcga acttacccgg caagttctgc ttcgcgcaat
     3601 gcccgatgcg ctttatctct ctggtggtga tgtcgcaatg gcaacagcca gtgcgttagg
     3661 cgcgaccgga tttcgtatta cggcgaaggt ggcgcaatgc gttccatacg gtcactttct
     3721 gggcggtgtc tggtcccgtt cggtgatgac gaaagccggt gggtttggcg atgagaccac
     3781 gttgcatcag gttctgaatt ttatcgagga gaagtgtagt gaataatatt attgcggtaa
     3841 cgatgggtga cccggcgggt attggcccgg agatcattat taaatccctg agcgaagccg
     3901 ggcttgctgg tgccccggtg gtggtggtgg gctgtgtgcg cacgctgcga cgtattatgg
     3961 cgatgaatat cacgccgcag gcagagttac gcgtcattca aaaggtgtgc gacgcccatt
     4021 ttgctcccgg cgtgattaac gtgatggatg agccgctggc taaccccgat gcgctgacac
     4081 ccggagtggt acaggctgcg gcaggcgatt tagcgtatcg ctgtatcaaa cgtgcaacgg
     4141 ctctggcctt atcaggcgag gtgaaagcca tagccactgc gccgctgaat aaagaagcgc
     4201 tgcatcttgg cgggcataac tatccggggc atacggaatt gctggcccat cttaccggca
     4261 gcaaagagta cgcgatggtg ctctataccg ataagctcaa ggttatccat atttcgactc
     4321 acatctcact gcgtaaattc ctggatacct tgaatggtga aagggtcaaa acggttattc
     4381 gtgttgcgaa ccacttcctg aaacgtgttg gtattgagcg tccgcgtatt gccgtggcgg
     4441 gtgtaaatcc acatgcaggg gaacatggtt tattcggcac tgaggaaatc gagattatcg
     4501 cgcctgcgat tgaagcaatg caggccgaag atattgatgt tactggcccg tgcccgccag
     4561 ataccgtgtt tatgcaatgc catgaagggc tattcgatat ggtagtggct atgtaccacg
     4621 atcaggggca tatcccttta aaactgcttg gattttatga tggggtgaat atcaccgccg
     4681 ggttgccatt tattcgcact tctgccgacc acggcaccgc gtttgatatt gcctggacag
     4741 gaaaagccaa gtctgagagc atggcagttt ccatccagtt agcaatgcag atttcgcgag
     4801 aataaaatga agggatataa ccggttagag cagatcatgg attacctgaa aagccataat
     4861 ctggtcaccg ttgatgagct ggtggcggta accaatgcgt cgccggctac gattcgtcga
     4921 gacctgataa aactcgatga gcagggtgtt atcagccgta cacacggcgg tgtgaccctc
     4981 aaccggttta ttcctaccca accgactacc catgagaaaa tgcagcgcag cctggcggaa
     5041 aaacatgcca tcgccagcgc agcggcgtca atggtcaaag cgggggattc cgtcgtcctt
     5101 gatgccggta cgacaatgat tgaacttgcg cggcagatta cacatttacc gcttcgcgtg
     5161 atcaccagcg acctgcatat cgcgcttttt ttggctgaat tcaagcaaat tgaagtgacg
     5221 attatcggcg gacgtatcga cgactccagc cagtcctgca ttggcgaaca tggtcgtaaa
     5281 ttgctgcaaa atacctggcc ggacgtggca tttttaagct gtaacagctg ggatcttgaa
     5341 aagggtatta cagcacccac ggaagagaaa gccgcactaa aacgcgatct tatcgcgcat
     5401 gcttcgcgca aaattctcct ggctgacagc tcaaaatacg gttcatggtc gctgtttaat
     5461 atcgcgcatc ttaatgaact gacagagatt attacggata gtcagcttga tgggcagacg
     5521 cgtagaacgc ttgcgtcctt gtcgacgcgt ttaatcatag ccgactaatc tatgactctg
     5581 cggccagctc atccggcaat ttaataaatg cgtcaggctg gcgacgtttg gccttacgtt
     5641 tccagcgcga ggatctgctg gcagtagcgc agaaaacgcc ccaatatctt acgcaaaacc
     5701 attttttctc taactaacag ttgtcagcct gtcccgctta taagatcata cgccgttata
     5761 cgttgtttac gctttgagga atccacgatg agtgaggcag aagcccgccc gactaacttt
     5821 attcgtcaga tcatcgatga agatctggcc agtggtaagc acaccacagt acacacccgt
     5881 ttcccgccgg agccgaatgg ctatctgcat attggccatg cgaaatctat ctgcctgaac
     5941 ttcgggatcg cccaggacta taaaggccag tgcaacctgc gtttcgacga cactaacccg
     6001 gtaaaagaag atatcgagta tgtcgattcg atcaaaaacg acgtagagtg gttaggtttc
     6061 cactggtctg gtaacgtccg ttactcctcc gattattttg atcagctcca cgcctatgcg
     6121 atcgaactga tcaataaagg cctggcgtac gttgatgaac tgacgccgga acagatccgc
     6181 gaataccgcg gcaccttgac gcagccgggt aaaaacagcc cgtaccgcga tcgcagcgtt
     6241 gaagagaacc tggcgctgtt cgagaaaatg cgtaccggcg gttttgaaga aggtaaagcc
     6301 tgcctgcgtg cgaagatcga catggcttcg ccgtttatcg tgatgcgcga tccggtgctg
     6361 taccgcatta agtttgctga acaccaccag actggcaaca agtggtgcat ctacccgatg
     6421 tacgatttca cccactgcat cagcgatgcg ctggaaggta ttacgcactc tctgtgtacg
     6481 cttgagttcc aggacaaccg ccgtctgtac gactgggtac tggacaacat caccattcct
     6541 gttcacccgc gccagtacga attctcgcgc ctgaatctgg aatacactgt gatgtccaag
     6601 cgtaagctga acctgctggt gaccgacaag cacgttgaag gctgggatga cccgcgtatg
     6661 ccgaccattt ctggtctgcg tcgtcgtggt tacactgcgg cttctattcg tgagttctgc
     6721 aaacgcattg gtgtgaccaa gcaggacaac accattgaga tggcgtcact ggaatcctgc
     6781 atccgtgaag atctcaacga aaatgcgccg cgcgcaatgg cggttatcga tccggtgaaa
     6841 ctggttatcg aaaactacca gggcgaaggc gaaatggtta ccatgccgaa ccatccgaat
     6901 aaaccggaaa tgggcagccg tcaggtgcca tttagcggtg nnnnnnnnnn gcgtctgcgt
     6961 aatgcttacg tcattaaggc agaacgcgtc gagaaagatg ccgaaggcaa tatcaccacc
     7021 atcttctgta cttatgacgc cgacacctta agcaaagatc cggcagatgg tcgtaaagtc
     7081 aaaggtgtta ttcactgggt gagcgcggca catgcgctgc cggttgaaat ccgtttgtat
     7141 gaccgtctgt tcagcgtgcc gaaccctggt gctgcggatg atttcctgtc ggtgattaac
     7201 ccggaatcgc tggtgatcaa acagggcttt gctgaaccgt cgctgaaaga tgcggctgcg
     7261 ggtaaagcat tccagtttga gcgtgaaggt tacttctgcc tcgacagccg ccattctacg
     7321 gcggaaaaac cggtatttaa ccgcaccgtt gggctgcgtg atacctgggc gaaagtaggc
     7381 gagtaagttt acgttgtcgg atagagcgtt tacgccgcat ccggcaagaa atatgcaaac
     7441 aatcccaaac gccgcgctct gcggcgtttt tttctgcttt tctt
//
LOCUS       DAWDRC010000129         7480 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4742_length_7480_cov_1.927407, whole genome shotgun sequence.
ACCESSION   DAWDRC010000129 DAWDRC010000000
VERSION     DAWDRC010000129.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4742_length_7480_cov_1.927407"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    530..539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1812..1821
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2575..2584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2873..2882
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3762..3771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6981..6990
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1924 a         1710 c         1899 g         1887 t
ORIGIN      
        1 gctaaatatt ccatcgccga tcaagtactt gcacgaaaag ctgccaaaca aagccaagct
       61 ggggctgtac tttaacccgt atggcaaagt gctggagctg atcgacgact gtatctcctg
      121 cggtgtggat caactgatcg acgccaatgg tggcccggtc tggacggaag aaggctttgc
      181 tgcgctgcat gaaaaagtcc gtgctgaact gaacgacaca gtggtggata ttgcgaagca
      241 ggtcgagcaa atcctcactg cggtgttcaa tatcaacaaa cgtctgaaag ggcgggtgga
      301 tatgaccatg gcgctggggc tttctgacat taaagcgcag atgggcgggc tggtgtatcg
      361 cggttttgtc actggtaacg gttttaaacg gttgggcgac acgttacggt atttgcaggc
      421 gattgaaaaa cgactggaaa aactggcgat tgatccgcat cgtgaccgtg ctcagatgct
      481 gaaagtcgaa aacgtccagc aggcatggca gcaatggatc aacaaactgn nnnnnnnnng
      541 aagacgtgaa agaggtccgc tggatgatag aagagctgcg cgttagttac ttcgctcaac
      601 aacttggcac gccttatccg atttcggata agcgtatttt gcaggcgatg gagcagatta
      661 gcggttaaca ctgctatttg cctgataaat ctaaaacccg gtaagcattt agcgccgggt
      721 tttttattaa ttctaaaaac gataagggaa aaaattcagg agttcagaaa aatacaattc
      781 tctgctgcaa gatgaataat gtttatctac agcatttcct taaaaaatat gtcaggcttg
      841 cggagtggca gttaaggaaa tacgacttcc tcctttcaga gtgctccgct tctcactatt
      901 atctcacgca gtattcttaa gggaaggata aggaggaacc atgaatatta ccccgtttcc
      961 gacactttcg ccggcaacca tagacgccat aaatgttatc ggacagtggc tggcgcagga
     1021 tgatttctcc ggtgaggtgc cgtatcaggc cgattgcgtg atccttgcag gcaatgcggt
     1081 tatgccgact atcgatgcgg catgtaagat tgcccgcgat cagcaaattc ctttactgat
     1141 tagtggtggt atcggtcact cgacaacttt tttgtatagc gccatcgcac agcatccgca
     1201 ctacaacact atccgcacca ctggcagagc tgaagctacc atcctggcgg atatcgctca
     1261 tcagttctgg catattccgc atgaaaaaat ctggattgaa gaccagtcaa caaattgcgg
     1321 tgaaaacgca cgctttagca tcgcgctatt gaatcaggcc gtagaacgag ttcatacggc
     1381 tatcgttgtt caggacccca ccatgcagcg gcgcacgatg gcgacgttcc accgtataac
     1441 cggggataat cccgatgcac cacgctggtt aagttatccc ggattcgttc ctcagttagg
     1501 aaataacgca gacagtgtaa tctttgttaa tccgttgcaa ggattatggc cggttgagcg
     1561 ttatctatcg ctactcactg gcgagctgcc gcgtttacgc gatgatagcg atggctacgg
     1621 cccccgcggg cgagatttta tcgttcacgt tgattttccg gcagaagtca ttcaggcatg
     1681 gcaaacgctg aaacatgatg cggtgctcat cgaggcgatg gaaagtcgct cgttacgtta
     1741 aaaattgccc gtttgtgaac cacttgtttg caaacgggca tgatttctga cttttatttc
     1801 tgcctttaat tnnnnnnnnn ncttgttttt atgaagccct tcacagaatt gtcctttcac
     1861 gatgccgtct ctctgatgat tgatgttaat taacaataaa tttaccaaaa acaaacatgt
     1921 aaatcacagg agtcgcccat gtcagtaccc gttcaacatc ctatgtatat cgatggacag
     1981 tttgttacct ggcgtggaga cgcatggatt gatgtggtaa accctgctac agaggctgtc
     2041 atttcccgca ttcccgatgg tcaggccgag gatgcccgta aggcaatcga tgcagcagaa
     2101 cgtgcacaac cagaatggga agcgttgcct gctattgaac gcgccagttg gttgcgcaaa
     2161 atctccgccg ggatccggga acgtgccagt gaaatcagtg cgctgattgt cgaagaaggg
     2221 ggcaagatcc agcagttggc tgaagtcgaa gtggctttta ctgcggacta tatcgattat
     2281 atggcggagt gggcacggcg ttacgagggc gagattattc aaagcgatcg tccaggagaa
     2341 aatatccttc tgtttaaacg tgcgcttggc gtgactaccg gcattctgcc gtggaacttt
     2401 ccgttcttcc tcattgcccg caaaatggct cccgctcttt tgaccggtaa taccatcgtc
     2461 attaaaccca gtgaatttac gccaaacaat gcgattgcat tcgccaaaat cgttgatgaa
     2521 ataggccttc cgcgcggcgt gtttaacctt gtactcgggc gtggtgaaac cgttnnnnnn
     2581 nnnntatgac aggcagcgtc tctgcaggtg aaaagattat ggcgactgcg gcgaaaaaca
     2641 tcaccaaagt gtgcctggaa ttggggggta aagcaccggc tatcgtaatg gacgatgccg
     2701 atcttgaact ggcagtcaaa gccatcgttg attcacgcgt cattaatagt gggcaagtgt
     2761 gtaactgtgc agaacgtgtt tatgtacaga aaagcattta tgatcagttc gtcaatcggc
     2821 tgggggaagc gatgcaggcg gttcaatttg gtaaccccgc tgaacgcaac gannnnnnnn
     2881 nnttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa agtggcgcgc gcagtagaag
     2941 aaggggcgag agtggcgttg ggtggcaaag cggtagaggg gaaaggatat tattatccgc
     3001 cgacattgct gctggatgtt cgccaggaaa tgtcgattat gcatgaggaa acctttggcc
     3061 cggttctgcc ggtggtcgca tttgacacgc tggaagaggc tatctcaatg gctaatgaca
     3121 gtgattacgg cctgacctca tcaatctata cccaaaatct gaacgtcgcg atgaaagcca
     3181 ttaaagggct gaagtttggt gaaacttaca tcaaccgtga aaacttcgaa gctatgcaag
     3241 gcttccacgc tggatggcgt aaatccggta ttggcggcgc agatggtaaa catggcttgc
     3301 atgaatatct gcagacccag gtggtttatt tacagtcgta atgagtgaaa gaggcggagg
     3361 ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt ttcgagggtg cgaataagct
     3421 gcgtgacgaa gccatatttg ttatcgtacc aggcgaccgt tttcaccagt tgtaaatcgc
     3481 ccacggcggt aatttccgtt tgcgtggcat caaacaccga accgaaatgg ctgccaatga
     3541 tatcggaaga gactatttct tcatcagtat aaccaaatga ctcgttattg tttgtcgcct
     3601 ttttaagcgc gttgttcacc tcttcggcag tcactttttt tccgagaatc gacaccagtt
     3661 cagtgaccga acctgttttc accggcacgc gttgcgcatg acctttcagt ttgccgctca
     3721 attccgggat caccagacca atggcttttg ccgcccccgt annnnnnnnn ngtggggaat
     3781 gatattttct gccgctgcgc gtgaagcacg taaatcttta ccacgtgggc catccaccag
     3841 tgactgggtg ccggtatagg catgaatggt tgtcatcgtg ccgacttcta tcccgaaact
     3901 gtcatgcaag gctttggcca tcggcgcaag gcagttagtg gtgcatgacg ccacggaaac
     3961 aatggtgtcg ttgccatcca gagtgtcgtc attgacgtta taaacgatgg ttttcatttc
     4021 accggcaggg gcggaaatca acaccttctt cgcgccagca tcaagatgcg cctgcgattt
     4081 ctcggcggag gtataaaagc ctgtacattc gacaatgatt tctgcgcctt tcgctttcca
     4141 cggaatattt ttagcctctt tttcggcgta aaccgcgata cttttcccat caacgataag
     4201 tgaatcttcc gtataatcaa tgctccaggg gaacggtccg tagtttgaat catgtttcag
     4261 caggtaggcg agaatttttg gggaagtgag atcattaata gcgacaacgt ctatgttgct
     4321 tttgacttca agtaatcgac gcaacaccag tcgaccgata cgaccaaaac cattaatacc
     4381 aactttactc atggtcttct cctgtcagga acgttcggat gaaaattgat cctttccagg
     4441 cttagaccag gatggcggga tgggcaatct acattcttgc agtgaaacgt aacgtactga
     4501 aaacgggtga acaatattta atgaaatttt aagaaaagcc cgttatgtta acggcaaatt
     4561 atgttaaagc aggaaatgtt atgggaaata aatactcaag gttacaaatc agcattcact
     4621 ggctggtctt tttgctggtt atcgtagcgt attgcgcaat ggagtttcgt ggtttcttcc
     4681 cacgtagcga tcggcccctc atcaacatga ttcatgtttc ctgtggcatc tcaattctcg
     4741 tagtgatggt ggttcgtctt ttgttaaggc tgaaataccc aaccccgccg attataccta
     4801 agccaaaacc gatgatgacg ggactggcgc atttgggcca tttggtgatt tatctcctgt
     4861 ttattgcgct gccggtgatt ggtttggtga tgatgtataa ccggggcaac ccgtggtttg
     4921 cgtttggttt gacgatgcct tacgcttcag aggccaattt cgcgcgggtt gatagcttaa
     4981 agtcgtggca tgaaacgctg gcgaatctgg gatattttgt catcgggttg cacgctgcgg
     5041 ctgcactggc acaccactat ttctggaagg acaactcact tctacgcatg atgccacgta
     5101 aacgttcctg aaggatattt aaagaaaacg cctgtactaa aaccgacccg tggtacaggc
     5161 gaagaatacg ggcctacatc ggaagcgcct attatattta tttgtatgac aaataaaacc
     5221 ataatccttg tctatacgtc catctggctt atctttaatc aattcacccg atctttgatc
     5281 tcatcaacgg tatcaaaata aaccagcgta taacgttcaa atcgaacata agattcgata
     5341 aatcatggag gttatatgaa aaaactggca cttatcctgg ttatgggaac gcttgtttcc
     5401 ttttatgccg atgccgggcg caaaccctgt tctggttcga aaggggggat ctcacactgt
     5461 acggcaggcg gcaaatttgt ctgtaatgat ggttctatta gtgcatcgaa aaaaacatgc
     5521 actaactgaa gtgtaaaagg ggtgccatga gaaaatgaat ccgctagtca gatgcgcggg
     5581 gaaaatcccc gcgcttgccc ttacctggac gtgcaggcca tgagcgcagc aacttcctta
     5641 tcaccgtccc ggaaccgcag ttcgtagagc gattgtcggg tcagtgtgaa tgtcagaatc
     5701 gtgttactct cgcaaggccg tggatttgcc gttgtcgcca gcgaagtacg gacactcgca
     5761 agccgcagcg ctcaggcggc gaaagagatt gaaggcttga tcagtgaatc agtcaggtta
     5821 attgacctgg gatcggatga ggtggcaacg gcagggaaaa ccatgtgcac tattgttgat
     5881 gccgtcgcga gtgtcacaca tatcatgcag gaaatcgcca ccgcctcgga tgaacaaagt
     5941 cgaggcataa cgcaggttag ccaggcgatt tctgaaatgg ataaggtgac gcaacagaat
     6001 gcttctctgg tagaagaggc ctcaacggcg gcggtgtccc ttgaagaaca ggcggcacga
     6061 ctaactgagg cggtggacgt attccgtctg aacaaacatt ctgtgtcggc agaaccacga
     6121 ggagctggtg aaccagttag ttttgctacg gtgtgaaaat gttaaaggag ggatcgacag
     6181 atcccttcac ctttcagaac ggcattgatt ttcgaatagc gttaatcatc aactggcaac
     6241 cagaagagaa cgtcgcatct acgcgggtca gtattccaat cggttcgcct gcaccatgtc
     6301 ccggaacagg cagggctacc agcgtggcat gacgcaggtc gtcttttaca gcgccagaag
     6361 ggacaaacca cacgtaatcg tattcaaccg taagttgacg agatagcgaa gcagacagcg
     6421 tttcgataca acccgaagga attttacatc cctggctctg cactaatgca tctgaatgct
     6481 ggcgtggcgc agtgccttct ggtgatacaa cgaccggcca ttccagcacc cggcttagcg
     6541 ttacgttctc ctgaagtagc gggtgattgg ggcggacaac cagcttcagc gattcaagaa
     6601 acagcagttc ataattaagc ccgttcatca gttcaggatc tgacatccga ccaatgccga
     6661 tatcgatttc cccggttttt aaacccgcca gaatcatagg gttactcatt gtcgcaactt
     6721 gcaaggtcgt ctctttttgt tgctgatgaa actgacctat aaccgaaggt aatatcccta
     6781 gtgccgcagt aggtagcgca ccaaccctga cgacatcatt attaagacct tctttacgat
     6841 gaagcgactg tccggcagtg ttgatggcgt caagaactct gactgcatgc gttaaaaatt
     6901 gttcgccggg taaggtaagt tgcgccccct gacgaccacg ctcaaacaag cgagcgcccg
     6961 tcagctgctc cagttcattc nnnnnnnnnn gagcgcaggt tgactcaaat taagggtttc
     7021 agccgcgcgc cccaaagttc cttgttgtgc gacagctacg aatgtatgaa ggtggcgcaa
     7081 acggatgcgc tgactaaaca gactattttt ttccataagc gatgttaaaa acgaagcggt
     7141 gtcgctgaca agtgaagttg ttttattatg ataacttgat tgcaaaatat tattaacaat
     7201 taaagcaact atgttacagc aaaatggata atattgatgt tttcgcggcg agatcacagt
     7261 ttgtaaattc ttccagccgg agtgaatgcg gttacctaca ctccagatta ctgaccactg
     7321 gaggcagaca ctatggcgaa cagcatcacg gcggatgaga ttcgggaaca gttttcgcag
     7381 gcaatgtcag ccatgtacca gcaagaagtt ccgcagtacg gcacgctgct ggaactggta
     7441 gctgatgtaa atctggcggt gctggaaaac aatcctcaac
//
LOCUS       DAWDRC010000130         7417 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4788_length_7417_cov_2.959114, whole genome shotgun sequence.
ACCESSION   DAWDRC010000130 DAWDRC010000000
VERSION     DAWDRC010000130.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4788_length_7417_cov_2.959114"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5245..5254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1979 a         1869 c         1567 g         1992 t
ORIGIN      
        1 cttcggtaat gatcaatttc atcttaatgc cttatattat ttgaataatt tctgtacgaa
       61 ggctgcatag gcaggccgcc agacatccat ttcatggttc agacccggat attcctggta
      121 atcaaactta atttttttct gctcaagctc agttttcagc ccggcgatat ccttgccggt
      181 tacaacatct ttatccccaa caaccacagt aaaattacgt agttgctggt taatagctgc
      241 cggctcgttc agccgggcag cgaccccttc atctggtacg gtcgtcgtgg taacaccact
      301 gaatgtggcc agccagccaa agctttccag atgattcatt cccgatacca gtgcctgata
      361 cccaccttgt gaaagccctg ccagtgcgcg accatcggca tctttacgga cattaaaacg
      421 cttgctaatc agcgggataa tatcgttcat cagttcgcga tctgccgctt tagcatttaa
      481 cggataaaag actttacgtc tctcctgagg aacgaaatct tcgggaataa tgcccttcgc
      541 atcggtttcg gtatccggga tcaccaccag catcggttta attttccctt cagccagcag
      601 gttatccatg atttgcggga tacgcccctg atcaatagcg gaacgtccgg tatcaccaaa
      661 gccgtgatag aaatagagca ctggcaaagg ctcgcctatg ccggtgtatc ctggcggggt
      721 ccagacatac atctgacgtt cggattgcaa tgcgttggag tggtaagtta tggcgatcaa
      781 atcaccatgc acaacagaac gtgtatccag ataactgccc ggtaccagaa tcatactgga
      841 gttaacctga cgctgaggct tagtcattgc cgtgcctgta tcaatgctgc gtacaccatc
      901 aacattgaaa aaatactcgt acagattgcc tttcaggaca ggcgtgcgcc acgaccagac
      961 tcccgcttcg tctttggtca tcgggtgaat attgtccgga accggaacac ccaccactac
     1021 ggagacattt tttgccccag gggcaaagta gcgaaaggtc acgctgttat cggtattgac
     1081 ctgagtcaca tactgtttca ccggaatagt gggtgccggg ctggctggca tatccgctgc
     1141 tatggcccat tgagcagaaa taccgctggc aatagccagc gttaaagcag caatttttat
     1201 attcatcgta atattccttt tattaagttc cattaccacc agatttcagc ctgggcaccg
     1261 acagcaaact ggtcgtcttt attgtcttca aaggtgaagc catccagttc gttttccaga
     1321 gctttgatat atgtagcgta gaaacgaatt tccgggcgcg aggtcaacat actggtattg
     1381 actttaaagg tatggaagag cgtggtttta taaccagact cattatattt attgctattc
     1441 gcatctttgt tttgctgggt gaaatagccc agttcaacac ctgtctggtt atattggtcc
     1501 caaatatagg ccgggcgaac aaccgcacga atagattcga aatcagaatg tgcgcctgtt
     1561 tcgtagctat aaatatcgtt accgaaggag taaacaatcg cgttagccac aatgaaatga
     1621 tcgccgatat aagcttcgcc ctgcgaagtc agacgaactg cagttccgcc tgtgtgatca
     1681 ccataataac gaccattaaa ggtagtaaat ggacttgcac cagcataacg accaaagtta
     1741 ctggcgatag agttattcgc aaccaggaag gagaattcgt tgaagccacc cttatcaaat
     1801 ttctgcgtta aagatgtacc aaacatccag gtatctttcc agtcataata cccgttatta
     1861 tcctgattat ctttttccga tgcgctttca tttgctgaga cataacgacc gctcaccatt
     1921 aaggtggctt tatcccataa cgggatatct ttatagcgta aatcaatggt attggtatta
     1981 atctgctgtt tgttttgcag gctgcgatcg taatcatcga catcttcacg aaccagcgcg
     2041 atatcaattt tacccggacc gactttccag ttttccagac ctacacctgc ggcagcatcg
     2101 gtacgctgcg ttttccagtc aagcatctgg atttcaattt tcggcgcacc gtgtttaccc
     2161 acccagaaat cagcctctgg cgcaaagggc aggaaacctt tggtggtaac gtacatatca
     2221 gagaactgca taaagttctc gccaccggcg ttatcgccaa accagccagt agagtactgc
     2281 tgacccacgt taccatccat catcacgatg gcatcaaccc gtttgccgtt ttcgttgtag
     2341 acacgttgtt ttaactgcaa atcaaaccag ccggagtatt cgttaccaaa gcggcccaga
     2401 gaaccaattg cccatgattt aggtgaacca tgagaggctg ttccccaacc agaacggtag
     2461 tagccgttgt aactaaagcc gatttcatct ttcacaaatt tactgaaatc tttcagagtc
     2521 atggaagaat aaatcgagcc tgtctcactg gcatttttct cttcattttt aaccagtaca
     2581 gcatcaggct ttgcggcatg actggtcggg aatggattgg cggcataccc ttgatcattc
     2641 gtacttacgc tacgattcac cgtcgctggc gtatactttt tcttctcttc atctttatat
     2701 tttttcagtt cgctttgcgt ttctcttaac gccttttcta atagctcaag gcgttgttcc
     2761 actgttaatg attcagccaa tgattgtgcg gaaaaggcta acggtgccat taataagatg
     2821 gcagaggtaa taatatttcg tctaaacata atatcccttt atggtgcaaa aaaagaatta
     2881 atgcatcgca tccagactgt tctgaatgcg acgataatta aggtgcttta ttggttattt
     2941 ttttaatgat agcccccaag ttttgatcac ttctgcatac catccgaagc ttttcttgcg
     3001 cgttcttgtg aggcttcctt caccattatc atcacgatcc acataaataa agccgtagcg
     3061 cttagacatt tgtgaatgag atgcactgac taaatcaatt ggcccccaac tggtgtaccc
     3121 cataatatcc acaccatcgg caatcgcttc atttacctgt accaggtgat cgtttaaata
     3181 ggcaattcga taatcgtcct gtatcgaacc atccacttca acgctgtctt ttgcgcctaa
     3241 tccgttctcg acaataaata acggtttttg ataacgatcc caaagcgtat ttaacagaat
     3301 ccgtaatcca accggatcaa tttgccaccc ccactctgaa cttttcagat gcggatttgg
     3361 gatcatattc agtatgttgc cctgcgcatt tttattaatg ctttcgtcgt gggaaacaca
     3421 accggtcatg taataactaa aagagatgaa atctacggta tgttttaaat cttctgcgtc
     3481 actttcagtc atctcaatgg tgatattgtg gtcgcggaag aaacgctgca tatagccggg
     3541 atactgacca cgcgcctgaa catcaccaaa gaacatccag cgccggttct cttccatggc
     3601 ctgcaacata tcctgtggct ggcaggtaag ggggtaaacc agcccaccga gaagcatatt
     3661 gccaattttc gcttcgggga tcaggctatg acaggcttta actgcccgcg cactggcaac
     3721 cagttgatga tggatcgcct gataaacttc cgcctcgcca ctctcttctg ccagccccac
     3781 gcccgtgaat ggcgcgtgta atgacatgtt gatttcatta aatgtcagcc ataacgcaac
     3841 tttatgttgg tagcgggtaa agaccgtgcg tgcgtaatgc tcgaagtgat cgatgaccgc
     3901 ccgattagcc caaccgccgt agtttttcac cagcccatat ggcatttcgt aatgggataa
     3961 cgttaccagc ggcttgatcc ccgcctgcgc catttcatca aacagccgct cgtaaaacgc
     4021 taaccccgct tcattcggtt cggcttcatc gccctgagga aaaattcgcg cccaggcaat
     4081 ggaaatacgc agacaggtga agcccatctc ggcaaataac gcgatatctt ccgggtaacg
     4141 gtgataaaaa tcgatggcga catctttgat attctctttc cccaggatgc gcggttccat
     4201 ttttcccatt acgccatgag gctgtaaatc tgaggtcgtg atccctttgc catcttcctg
     4261 ccaggcgcct tccacctgat tggcagctgt tgcaccaccc caaagaaatg tttctggaaa
     4321 tgctttcata attaactcct tttatcgtta gcgaatgatg gataacagcg gctcaccagc
     4381 gtttatctgc gccgtgccgt ggggtaatac gtccgtaaaa tcatcgctat tactgattaa
     4441 taccggcgtc gtcagatcaa atccggcctc gcgaatagca gggatatcaa aagaaatcag
     4501 ccgatcgcct gtattgacct tgtcacccac gttgacgtga gcggaaaaga atttgccgtc
     4561 cagttttacg gtgtcgatac cgacatgaat caggatctcc acgccatcat ctgactcaat
     4621 gccaatggcg tgtaatgtgg cgaacaacga agcaatttgc cccgccaccg gagaacgcac
     4681 ttcaccaaca gagggcagaa tggcaatacc tttacccaac aggccgctgg caaacgtggt
     4741 atcagcgacg tgaatgagcg gcacaatatc tcccgtcatc ggtgaacaga taccgccctg
     4801 ctcaggtgta ataacctctg gtgttttctc ttgctgggca acttgcgctg actgactctt
     4861 agcggtgatg aaatgaaaca tcaccgtacc gacaaatgcg caaccgatgg caatgacacc
     4921 gccaataacg ctggcccata cggtgaaatc aatccccgtt gacgggatgg tttgcatgaa
     4981 ggtgaaaata cctggcaaac caaaggagta aactttcgtt tgcgcgtagc caatgatggt
     5041 ggcccccaat gccccactta tgcaggcgat aacgaagggg tacttacgcg gcagattgac
     5101 gccatatacc gctggttcgg tgataccaaa caaacctgtc aacgccgctg atcccgccac
     5161 cacttttttc tgtgcatcgc gttcgcagag gaagacgccg agcgccgccc cgacttgccc
     5221 cataatggcg ggcattaaca gcggnnnnnn nnnnattaaa ggtatcaaac cccaatgcag
     5281 accgaacatc acaaagattt gccagaagcc gcccattacc gcgcctgcaa atgcaggaac
     5341 cgcctgataa agccagagat aaccggcagc aatcagttca cttatccagg ttgatagcgg
     5401 ccccaccagc agaaatgtga tgggcgtgat gaccatcaga catagcaagg gtgtgaagaa
     5461 atttttgatt gccgaaggta accacgcatt aagtcggcgt tccagaatgc tgcacaacca
     5521 ggcagaaaaa ataatgggaa taaccgatga cgagtaattc aacaatgtga ccggaatacc
     5581 caggaaatcc aggcccagcg catccacttt ttgcccgttc tcgaaggcag tcagaattaa
     5641 tggatgcact aacgctccgc caatcaccat ggcagtaaat ggattgccgc tgaagcgttt
     5701 ccctgcggtg tagcccagga ttatcgggaa gaaccaaaac aaggcatcac tggcgctaaa
     5761 taaaattaaa taagtaccac tttgttcggt cgtccactga aaagtgagtg ccagagccaa
     5821 catacctttc aggatcccgg ttgccgccat caaaccgatc agaggcgtaa aaatacctga
     5881 aataacataa acaaagcggt ttaacagatt atctttatca tcattttccg gtgcctgttg
     5941 cgctttttcg ccaaggcctg ccacgctgtt aaccgccagg aagacatcgg ccacatggtt
     6001 acctatgaca acctgaaact ggccaccgct ttccaccacc ataataatac cgggggtctt
     6061 tttcagtacc tcagcttgcg ctttgctttc atcctttaac ttaaaacgta atcgcgttgc
     6121 acaatgcatc agactcacaa tgttatctgc gcccccgact cctgcgacta ttgttctggc
     6181 taactccgtc ataacttgcc ctctaccgct ttgcggcaaa actccaaaaa aaaacctgaa
     6241 aaaaacggcc tgacgtgaat caagcaattt ttttcaggtt ttgcccgctt agtgcggtaa
     6301 caatccttta ttcagtaata atatttcagt gttctttgcg cacgcgctct atatttatgg
     6361 ctaaaaacat aatctctgca ggtgagattt tacgttgata ctgcaaacca ataaaaatgg
     6421 cgatccgctc cgcacattgc catgcttgcg ggtaattttg ctttactgct tgttgtaatg
     6481 attcatcact atcgttaatc gatgcatgtt caagaatacg ccaggataaa aatttcagat
     6541 gcgtaaccag tcgctgataa ctcaagcttt cttcctggta attaaggctg aactgaaatt
     6601 ttattaattg cagcatttct cgcattaatt gcgtgacacc tgcaacatcc tccatatttc
     6661 cgctcatttg ggcactgacc agatgcatgg caataaagcc cacttcatct ttcggtaact
     6721 gcacgcccaa ccgtttatca ataatggtta acgcttcttc ccctagctgg aactctttcg
     6781 ggtaaagccg ctggatatcc cacagcagcg ggttgggcag tagcacgttt tgctgaaagc
     6841 gtttaatcgc aaactggcaa tggtcagtta gcgagatata aatactgtcc tgcaactttc
     6901 ccaggcgctc ctgcgccaga gagataatac gatcacaggt tgccatcacc tcaagaggca
     6961 tatgacttaa gagttcgctt aatcgcccgt tcagttcatg actgctcaag gcatactctt
     7021 tttctattcc acttgagtta attctttcgc ctgggcgttt ttgaaagcca attccgcgcc
     7081 ccatgacgac tttttcccgc tgttgatcat caataaccac cacaacatta ttgttgagaa
     7141 ttttggtgat ttgcatgttc atagcaagga ccttttttat aaacaaaaaa acccgacttc
     7201 accagtattc tctggttatg tcaggttttg cctgcgaatg cagtaacaat ccagtcatta
     7261 attaatggtt tttataacga acatccaggt tcgaaaatta atttagttgc gtgcagagaa
     7321 aatagcaatg ctctatttat aaaagtatga ccatgctcgc agttattaat tttgtgtaat
     7381 tttaggattt tataaagtta tatataacaa atcccaa
//
LOCUS       DAWDRC010000131         7411 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4794_length_7411_cov_2.117319, whole genome shotgun sequence.
ACCESSION   DAWDRC010000131 DAWDRC010000000
VERSION     DAWDRC010000131.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_7411_cov_2.117319"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2070..2079
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5499..5508
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6364..6373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    7020..7029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1844 a         1929 c         1802 g         1796 t
ORIGIN      
        1 tgtagaggtt gttaaacaca tactcagcgc gttcccggtt cagagtcagc tctttgcgcc
       61 cttcgagtac ggtttgaaaa tccgtgtaca gcttgtcttc ggtttcacgc agggtcgcca
      121 tatgtttgta cacccgcgcc accagcacaa aaccacccca gatggtgact gccatccaga
      181 tagccgttac cagcaacatt ttgcccgaca gcatccacaa gtacgccgcg gaaccgatag
      241 tgaggatgat cccctgcacc agttccggca gacgcacaaa agcaatggtg atattgcgca
      301 catcgctggt taatcccgcc agcaacgagg cgctaccgag ttgttcaatg cgctcgacgt
      361 gagtatcgag aatccgcttg ataaattcac tacgcagtcg gtaaacgaag tgatgcccca
      421 aagtggtgag cgccagttgc gatccgagag tgacggccat caacagcagc aataacccca
      481 gaaactccgg caataccagc agactggtat ccgccgtttc gataaggcgc tgattgataa
      541 aagcaattaa gccaatgcct aatgccgcac tggcgaggct tagcgccatc acactgataa
      601 atggccagcg atactgccgc cagacaagta caagaagttc catgcagaaa acccggacaa
      661 tgaattacag cccgcagttt aaacatcttc gcgcgcacag caataataat tcttattttt
      721 attctttttt acctgcctga cggaatgtca ggttgtagcg gcagtcgccg gtgagtggat
      781 gaaaccccgc tttcaacggt tgaatcccat gataaaacag ccgcgactca ccgccccata
      841 ccaccacatc gccatgttcc aacaacaatc tttttagcgg atcatttcgt ttcaggccgc
      901 caaattgaaa aatcgcgggt aagcccagag aaacagaaac aattggcgcg cgcagatccg
      961 gttcgtcttt atcctgatgc agcgacagtt tcgcgccagg aacgtagcgg ttgatcaaac
     1021 aggcatctgg ctggaaatct ggatagcccg ccgccgtagc cgcacgttga cataaatgat
     1081 gaaaactctg cggcatggcg ggccacggtt tatttgtttg cggatcaatg ggcgaataga
     1141 gataaccttg ccgatgggtc gtccagccca gatgcccaca gttggtcatc gccaccgaca
     1201 tggtatatcc gcctggagtg accatctggc gaaacggcga ctggctggca acgtcattaa
     1261 tatcgcggat cagttgctcc gcagcgttaa aagcaaaacg ccgtaaaatt accgcaccag
     1321 ccgccagtgg ctcttgccac ggttcagcat cggcaaacag atccaacatt acctctcctc
     1381 attctcagct tcgcggcgca gcagttgcgc tttacgcgac acgccccagc ggtaaccgga
     1441 aagtgtgcca tcaccacgga ccacccgatg acagggtatt atgatagcca gcttgttggc
     1501 ggcacaggcg ctggcaacgg cccgtaccgc tttcggtttg ccgatggcat tagccagttg
     1561 ctgataactg acggtttcac cgcaaggtat cgtgcgcagt gcctgccaga cttgttgctg
     1621 aaaagcagtg ccgcgaatgt ccagcggtaa cgtcagcggc gtatcgcgtt gattgaggct
     1681 ggcgatcact tcacgcacat gttgctggaa cgtaagatca gcaggcgcgc tgtcggcagc
     1741 gggaaacatc tgctgtaact cgctgattag tgtcgcgtca tcatcgccca gtaatatcgc
     1801 gcaaatcccc cgctcacttt ctgccaccag gcaacggccc agctcacaat cagccagcgc
     1861 gtaacgcacc gccagatttt cgccaccgtg acggaattgt ttagccgtca tgcccagcgt
     1921 ttcgtcggct ttgcggtagt aactgctgct gtcggggaat ccggcgttaa gaatagatgt
     1981 agtcacgctc tcccctttcg ccagcaattc gcgcaaacgg cgagcgcgcc aggcctgttg
     2041 ccaggcttta ggcgtcattc cggtagtcgn nnnnnnnnnc cgatgcaaat gaaatggact
     2101 catcgccacc tggtcggcta aggcttccag cgttacaggc gtttcctgtt ccagaagtcg
     2161 gcaggcgtgg gtaattttat ctaggcgatg ttgcagggga ttggctttgt ctggctgaca
     2221 acgtttgcag ggacgaaagc cagcggcaag cgcctcgctg gcatttgcgt agaaggagac
     2281 attttcccgc aaggcatgtc tggctcggca agacggacgg caaaagatac ccgtggtgcg
     2341 cacggcgaaa acgaattcgc cgtcggcgtt cgggtcgcgg gctaagacag attgccagcg
     2401 ttgatcgtca gttaaacatg tggcgttttt cataatcagc tccctggtta aggatagccg
     2461 ttagtctgcc cggtcaccat cgcgcaaaaa ccaacaatct tgcgctttaa tttttttcgc
     2521 tgataaggaa gcttttaaac tgcggcgaca tccaggtttt aaaattatcg ccctctttgg
     2581 tgatcatgta gaccgccagt ccttcccggc gaacaacctc tttggctttc tccggtccga
     2641 gtaccattaa gccagtatcc caggcatcgg cttccagcgc cgtcggggca atcaccgtca
     2701 cggatacgag attgtgttcg ataggacgcc cggtttgcgg atcgataaca tgagaaagac
     2761 gtttgccgtc cagttcgtaa tagttacggt agctgccaga ggtactaatc ccatgaccgt
     2821 tgatatccac cacggcctga accgcgtttt ctttatcggt tggcttttga atcgctaccc
     2881 gccacggctg gcctgcaccg ttcataccac ggctgttcag cgcgccgccc accgacacca
     2941 gatagcggga aatcccttcc tgctccatca agcgtgccag gtgatccgcc gcataacctt
     3001 cgccgacggt ggagagatcg acatataaat ccggcaggtc tttttgcaga tattgctgat
     3061 gcgactgatt aatgaccgtc aggtgctgta agccggtttt ggctttcatc gcatcgatct
     3121 gttcctggct cggaatttga accggttgtt gttccggacc aaagccccac agattcacca
     3181 gcggcccgat ggttatatcc atcgcaccat cggtccttgc gccaatgcgc agcgaggtgg
     3241 tgacgatatc ggccatcgct tcacttaccg gccacggcga caaactttgc gagtcgttaa
     3301 agcgcatcag cgcggaatct tttttatagg tcgaaagcag ctgatcgtca gcgtccagtt
     3361 gggtctgaat cttttcttta agttcggcac tgcgtttggc gtcaatgccc gggatgctgg
     3421 cacgccagaa ggtacccatg gttttgcctt caagaacggt aacttcagta gcgtgggttt
     3481 tggcgggttg tggtttttga tcgcaaccaa caaagaagag cgcggcagcc agcagtgcca
     3541 cgcgggtaaa gcttatttcc atacgtgatt atcctcatgc gaacggtcgc aagagtacac
     3601 caaaaaactg tgtttgtacg ctgaaaacaa tgaaaaaagg gcccgcaggc cctttgttcg
     3661 atatcaatcg agattagaac tggtaaacca ggcccagagc tacgatgtta tcagtgttga
     3721 tgccagcgtc acgagtgaac tggttgtcgt ccagcaggtt gattttgtag tcaacgtagg
     3781 tggacatgtt tttgttgaag tagtaggtcg caccaacatc aacatatttc aggatatctt
     3841 cgtcgtcgta gttacggcca ttgatgacac ccaggttttt acctttagac tgcaggtaag
     3901 ccagggacgg acgcagaccg aagtcgaact ggtactgagc aacagcttcg aagttctgtg
     3961 ctttgttcgc ccaacccagg gaacctacgc gagttgcgtt gtaggtctgg gtgtactgag
     4021 cagccaggta gatgttgtta gcgtcgtatt tcagaccacc agtgtaggtt tcagcacggt
     4081 cgccagtacc gatgtagtta tgagttacat tatcaactac gtaagtacca ttctgagcgt
     4141 cagtacgttt ggagctggac actgcagcac cgataccgaa gccttcgtaa tcataagtga
     4201 tagaaccgcc aacgccgtcg ccgttctgac gcagagcacc acgaccgttg ttggtcatgc
     4261 cttcgccatc tacgctaccg tttttaccct ggtactgaac agcaaagttc aggccgtcaa
     4321 ccagaccgaa gaagtcggtg ttacggtagg tcgcgaagcc gttaccacgc tgctgcatga
     4381 agttgtcaga accgtaagtg tcgccaccga attctggcag gacgtcggtc caggaagtta
     4441 cgtcataaac aacgccgtag ttacgaccgt agtcgaaaga acccacatcc tggaatttca
     4501 gacctgcgaa tgccacacgg gtccaggagt tgttttcgtt ttcagcgctg ttgccctgga
     4561 tctgatattc ccactggccg taaccggtca gttggtcagt aacctgagtt tcacctttga
     4621 agccaagacg catgtaggtc tggtcgccat ctacagactt gtcgtcagag aaatagtgca
     4681 ggccgtctac tttaccgtac agatctaatt tgttgccgtc tttgttgtaa acttcagcag
     4741 cgtttgctgc gcctgctacc agcagagctg ggaccaggag ggacagtact ttaactttca
     4801 tgttattaac cctctgttat atgcctttat ttgctttttt atgccactgc atactgatta
     4861 accctcatta atcagtcggc aagtccattc tccccaaaaa tgcagaataa tccaacacga
     4921 atatgatact aaaactttta agatgtttca tttatcgcta tagatgtttc agaatgtaaa
     4981 tgaaagggaa ctttttaaga ttattgcgga atgaccaaat aagcacctaa catcaagcaa
     5041 tattaattca aggttaaaat caataactta ttcttaatta tttgacagca ctgaatgtca
     5101 aaacaaaacc ttcactcgca actagaatat cttccgctat catcattaac tttatttatt
     5161 accgtcattc atttctgaat gtctgtttac ccctatttca accggatgcc tcgcatccgg
     5221 ttttttttac ccttctttac acacttttca ttattctgtg ctaccacaga aaaactataa
     5281 cgcctgttaa ctattgcaca aataattaac ataggcataa tttccagcaa tcttcgttta
     5341 tttgcaatta atatcgttag gctttttgta ggttatttgt acagcaaaat ggcgcttgta
     5401 catctatttc acccaatgca ggatgataaa tatcactgga gaatagagaa tcatcaatca
     5461 ggtaagagtc tggaatttca cactgtaccc tttatactnn nnnnnnnnta ctgccctatc
     5521 acttcgcgaa gttttaacag gtcataaaca cgaatgcgtc agaaagagac aacggccacg
     5581 acccgctttt cactcctacc ggggagcatt acccgcttct ttttactgtt gatcattgtg
     5641 ttactggtga cgatgggtgt aatggtacaa agcgccgtta acgcctggct gaaagataaa
     5701 agttaccaga ttgtcgacat tacccacgca atccaaaagc gcgtcgatac ctggcgttac
     5761 gtgacctggc agatctacga caacattgcc gcgacgacct ccccctcctc cggcgaaggt
     5821 ttacaagaga cgcgcctgaa acaggatgtc tactatctgg aaaaaccacg ccgcaaaacg
     5881 gaagccttaa tctttggctc tcacgacaac tcaacgcttg agatgactca acggatgtcc
     5941 acctatctgg acacattgtg gggcgcagaa aatgtaccgt ggtcgatgta ttacctgaat
     6001 ggtcaggata acagtctggt gctgatctca accctgcccc tcaaagatct cacctccgga
     6061 tttaaagaat cgaccgtcag cgacattgtt gattcacgtc gtgcagagat gttgcaacag
     6121 gccaacgccc tcgatgaacg cgaaagtttt tctaacatgc gccgcctggc ctggcagaac
     6181 ggtcattact ttaccttgcg taccacgttc aaccagccgg gacatctggc aacggtcgtg
     6241 gcttttgatc tgccgattaa tgatttgatc ccaccgggta tgccgctgga cagtttccgc
     6301 cttgagccag acgcgacggc aacgggaaac aatgaaaatg agaaagaagg gacggatagc
     6361 gtcnnnnnnn nnngtatcca ctttaacagt acgaagattg aaatctcctc ggcactcaac
     6421 tctaccgata tgcgcctggt ctggcaggtt ccttacggca ccttactgct ggatacgttg
     6481 caaaacattc tgctgccact gctgctgaac atcggtttgc tggcgctggc gttatttggc
     6541 tataccacat tccgccattt ctccagccgc agtacagaaa gcgtccccag cacggcggtc
     6601 aataacgaat tgcgcatttt acgggcaatt aatgaagaga tagtctcact gctgccgctc
     6661 ggcctgctgg ttcacgatca ggaatcgaac cgcacagtca taagtaacaa aattgccgat
     6721 catttgctgc cgcatttgaa tctgcaaaac atcaccacca tggcggaaca gcatcagggg
     6781 attattcagg cgacgatcaa taacgagctg tatgagatcc gcatgttccg cagccaggtt
     6841 gcgccgcgca cacaaatttt cattattcgc gatcaggatc gcgaagtgct ggtaaacaag
     6901 aaactcaagc aggcgcagcg tctgtatgag aaaaaccagc aggggcggat gacctttatg
     6961 aaaaacattg gcgatgcgct gaaagaaccc gcacagtccc tggcggagag cgcggctaan
     7021 nnnnnnnnnc cccggaaggc aaacaactgg cgaatcaggc tgatgtgctg gtgcgactgg
     7081 tcgatgaaat acagttagcg aacatgcttg cggacgatag ctggaaaagt gagacggagc
     7141 tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct gccatcaagc
     7201 gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg cgccgcggcg
     7261 atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg acctcaacgc
     7321 aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctccgaagac cgcctgacgt
     7381 tccgcattct ggacactgga gaaggcgtaa g
//
LOCUS       DAWDRC010000132         7397 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4800_length_7397_cov_2.473304, whole genome shotgun sequence.
ACCESSION   DAWDRC010000132 DAWDRC010000000
VERSION     DAWDRC010000132.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4800_length_7397_cov_2.473304"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1717 a         1824 c         2001 g         1855 t
ORIGIN      
        1 gttatataat tgtatgatga atataaacat gcataaaaat aacgagtttt actaatatta
       61 caaattttta tagtaatttc tcatgtacat atttttttac aggctaaaaa aagtataaat
      121 aacaggcaaa aaaaagcgcg gggtgggacc gcgcaaaaag catgacattt tttgtttgca
      181 gcaattttta tgaaaacgtt gccatactta aaaggcctca agccaggcgg cgtaatgtta
      241 tttaacaatt acgccttcag cggaatagtg gttacgcttt cacacacata acgtggtaaa
      301 taccgtcaat aatttctgtc ccttctgttt cgtgttcaaa tccagggaaa tgatggtccc
      361 aggattgcag cgagcgtaaa taacttactt gcggactgtt tttatcgccg aagttttcac
      421 cagacagcag catcgggatt cctggcggat acgggataac tgagtttgcc gcgatacgtc
      481 ctggcagatt ttcaatggat accagttcga cattgttgtc gacaatcgcg ttgtacgctt
      541 cacgcggggt gatttccgcc accggtaggc cggaataggc ttcattcaac cgtgcgccag
      601 ggttgttttc tttcagccag gcaaacatgg tgtcacccag atcgtgaatc cctatgttcg
      661 cgtaagtgtc aggatattgt tcaacaagtt ccggcatcac ctgcgccagc ggtgtgttgg
      721 catcatagtg gcgtttgaag gagcaaaggg tgttaaccag agttccccat ttcccacggg
      781 tcacgcccat agagaacagg aacataatct ggaagtcagt ggtacgggta ggcacaatgc
      841 cgtggcgacc aagccaggct gtgaccagcg ccgccggaac accggtttct tccagttcac
      901 catcttcacc cattcccgga gcaaggatgc tgactttaat cgggtcgagc atactccagt
      961 tatccggaat atctttgaag ccgtgccagc tttcgcccgg atgcatgacc cagcagtcct
     1021 gaacggtggt cagcagtttg gttggtgcgt cagcaaagtc ataggttttg ccggtttgtg
     1081 ggtcggtgac gacttctttg ttccacggtt tgaagaacca gctaccgtca gcggcgaact
     1141 ctttatatag ccgcgccatc gcctgacgga aatcaaccgc ttcgtcgatc acttcctggg
     1201 tcagtgacag gccgctgttg ccgtccatca tcgacactgc cacgtcgttg gatgcgcaga
     1261 tggcatacag cggggaggtg gtggcatgca tcatgtaggc ctggttgaag cgggagaagt
     1321 taatcgcccc acgaccttca cgtacatgaa tataagaggc ctgggacagc gcattcagca
     1381 gtttgtgggt ggagtgggtg gcgaaaacgg taggaccgtt gtgatcgcca ggttcgccgc
     1441 gcatggcata gtgatcggca tagatcgggt tgaaacgtgc atagccgtac caggcttcgt
     1501 caaagtgcag acgatcggag gttttttcca gcagatcctg cgcttcttta gcgttataac
     1561 acacgccgtc ataggtacag ttggtcacca cgcagtaaga cggtttttgc ccgactttgt
     1621 ctttggtcag cgggctttca ctgattttct tctgcaaggt ttcaggttgc atttcctgcg
     1681 gatagattgg cccgataatg ccgtagcggt tgcggcttgg caccatgtag accggtttcg
     1741 cgcctgtcag catcaagcct tgttcgatgg atttatggca gttacggtca acgaccacga
     1801 catcgttatc ggtcatgcaa gcctgcatga tggtgcggtt agagccggaa gtaccgacga
     1861 ctaccgacca ggagcgatcg gcaccaaata cgcgtgcggc atatctttcg ctttcgccaa
     1921 atgcgccagt atggtcaagc aaagaaccga gggaagtgcg ttcgatgccc atgtcggtgc
     1981 ggaacagatt ttcaccatag tagtcatggt agaaacgtcc ggcgggtgtt ttggtaaaac
     2041 caacgccgcc ctggtggcct ggcgctgccc aggaatattc atggatgtcg ctatatttca
     2101 tcagcgcgct gaacagtggc ggcaatagct gctggcggta gcgggtcatc gcggcaacgg
     2161 cgcgtccggc gataaagtcg gcagtatctt ccagaatcca ggcgaattca tcgacaagct
     2221 ccagcaggtc gcgatccatt gcggcgaggg ctttttcccg atcgcccaac aggaagaccg
     2281 gcacgttttg ttggcgctca tgaagcttac cgatcaattg tctgacgttt tgatgttcgt
     2341 ccggatgttc catttgatag ctgaacatca ggcagtcaat ggcttcgttt gaagagagaa
     2401 tgacaaaacc atcatcaaag gaggtggatt taatcacggt aacattttgc tggcttaaag
     2461 catctgccag acgctcaacg gcgttaccga cccaggtgtc ttgatggaga aactcgcttt
     2521 caacaattaa tactttcatc attgcttacc cggttatgaa ggaatcttcg ttgcgggcaa
     2581 gtatcttccg gccttttcgg gctgtaaagc gcgtgaaaaa caaacatttt tggaatattt
     2641 atttttataa acaatacgtt atgtgattat tttaaatcat tttcactgct tattatcgac
     2701 ggctaaacta tttttttggc tgatactgat atcgtcttta gccgggaaac gtctggcggc
     2761 gctgttggct aagtttgcgg tattgttgcg gcgacatgcc gacatatttg ccgaacgtgc
     2821 tgtaaaaacg gctactcgaa cgaaagcctg ccgtcagggc aatatcgaga atacttttat
     2881 cggtatcgct cagtaacgcg cgaacgtggt tgatgcgcat cgcggtaatg tactgtttca
     2941 tcgtcaattg catgacccgc tggaatatcc ccattgcata gttggcgtta agtttgacgt
     3001 gctcagccac atcgttgatg gtcagcgcct gatcatagtt ttcggcaata aagcccagca
     3061 tctggctgac ataaaattgc gcatggcgcg agacgctgtt tttgtgtgtg cgcgaggttt
     3121 tattgaccag aatcggttcc cagccagaga ggctaaatcg cttgagcatc aggccaattt
     3181 catcaatggc gagctggcga atttgctcgt tcggactgtt taattcctgc tgccagcggc
     3241 gcacttcaaa cgggctaagt tgctgtgtcg ccagtgattt gatcaccatg ccgtgagtga
     3301 cgtggttaat caggtcttta tccagcggcc aggagagaaa cagatgcatc ggcagattaa
     3361 aaatcgccat gctctgacag gttccggtat ctgttagttg gtgcggtgta caggcccaga
     3421 acagcgtgat atgaccctga ttgatattca ctttttcatt gttgatcagg tattccacat
     3481 cgccatcgaa aggcacattc acttcgacct gaccatgcca gtggctggtg ggcatgatat
     3541 gcggtgcgcg aaactcaatt tccatccgct ggtattctga atacagcgac agtgggctac
     3601 gggtctgctt ttcgtcgctg ctgcacataa acgtatctgt attcatggat gactctcttt
     3661 cctggaatat cagaattatg gcaggagtga gggaggatga ctgcgagtgg gagcacggtt
     3721 ttcaccctct tcccacagcg gcgaggggag cgtccgagca tcatgaggcc gaaaactctg
     3781 cttttcaggt aatttattcc cataaactca gatctattga tgtgccactt aggatctgag
     3841 tttttgggaa tgctcaaccg ggaaaccgga ggttttctgc gatatcgcct gccatgatga
     3901 agtcattcaa gcaagccagg agatctgcat gatgtctgca cctaaaatta catttatcgg
     3961 cgctggttcg acgattttcg ttaaaaatat tcttggtgat gtgttccatc gtgaggcgct
     4021 gaaaacggcg catattgccc tgatggatat cgatcccacc cgcctggaag agtcgcacat
     4081 tgtggtgcgt aagctgatgg attcagcagg ggccagcggc aaaatcacct gccacaccca
     4141 acagaaagaa gccttacagg atgctgattt tgtggtggtg gcatttcaga ttggcggtta
     4201 tgaaccttgc acggtgactg atttcgaggt ctgtaagcgg catggtctgg aacaaaccat
     4261 tgccgatacg ttggggccgg gcggtattat gcgcgcgcta cgtaccattc cgcacctgtg
     4321 gcaaatttgc gaggacatga cggaagtctg ccccgatgcc accatgctca actacgttaa
     4381 cccaatggcg atgaatacct gggcgatgta tgcccgctat ccgcatatca agcaggtagg
     4441 gctgtgccat tcggtgcagg gaacggcgga agagctggcg cgtgacctca atatcgatcc
     4501 ggctacgctg cgttaccgtt gcgcaggtat caaccatatg gcgttttacc tggaactgga
     4561 gcgtaaaacc gcagacggta gctacgtgaa tctctatcct gaattgctgg cggcttatga
     4621 cgcagggcag gcaccgaagc caaatattca tggtaatact cgctgccaga atattgtgcg
     4681 ttacgaaatg ttcaaaaagc tgggctactt cgtcacagaa tcgtcagaac attttgctga
     4741 gtacacaccg tggtttatta agccaggtcg tgaggatttg attgagcgtt ataaagtacc
     4801 gctggatgag tacccgaaac gctgcgtcga gcagctggcg aactggcata aagagctgga
     4861 ggagtataaa aacgcctccc ggattgatat taaaccgtca cgggaatatg ccagcacaat
     4921 catgaacgct atctggaccg gcgagccgag tgtgatttat ggcaatgtcc gtaacgatgg
     4981 cttgattgat aacttgccac aaggatgttg cgtggaagtg gcctgtctgg ttgatgctaa
     5041 tggcattcag ccgaccaaag ccggtacgct accttcgcat ctggccgccc tgatgcaaac
     5101 caacatcaac gtacagacgc tgctgacaga agctattctt acggaaaatc gcgaccgtgt
     5161 ttaccacgcc gcgatgatgg acccgcatac tgccgccgtg ctgggcattg acgaaatata
     5221 tgctcttgtt gacgacctga ttgccgccca cggcgactgg ctgccaggct ggttgcaccg
     5281 ttaaggggta atcgcttccg gcgctctggt gattaagcca aagcgccgta gctggcggca
     5341 atgaaagcgg gcgaggaata tcaccgtcgt taatgcgccc agtagtgcgc agaacatatc
     5401 ggattgcgta tcccacgggt cgccttgagt accgagaaaa tcatctgcgc cctggcccat
     5461 tgccagcgct gcccaccact cgattaactc atacatcgcg cttatcgcca gcgcgacaca
     5521 acagaccaga aacgccacca ttttacgccc gcgaacgtac atcccgcgca cgaggatctc
     5581 tcgtgccacc agggcaggca ccagcccctg gaaaaaatgc cccagtttgt catacggatt
     5641 acggctcaac cccagccatt cctgcacctc aaaaccaacg gggactttcg cgtaggtgta
     5701 ttgcccgccg accatcagga tgatggcgtg aaagaaaatg agcgtataga gcagcggcgt
     5761 taacgggtaa cgtttagcgg tggcaagcag tagcggcacg acaataatca ccggtgtcac
     5821 ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg gtataaatca ggattaacgt
     5881 tagcgccagt gcgccggtgt taagaattaa cggcttgagt gtgcgggtca tggtgagttc
     5941 gcaagtcagg gaaaatactg actattgacc agtaagcgtg aaattacaat cgcaaaagag
     6001 gtggcccagg gggatcacct ggcagcatgc tgccaggcgc tgggccgaag aggttactta
     6061 gtgcagttcg cgcactgttt gttgacgatc tgctggaaga agtcgttacc tttgtcatcg
     6121 accaggataa acgccgggaa atcttctact tcgattttcc agatagcttc catacccagc
     6181 tccggataag cgacgcattc cagatgcttg atgctctgct gcgccagtac cgccgccgga
     6241 ccgccgatgc taccgaggta gaagccgccg tgtttatggc acgcgtcggt aacctgctgg
     6301 ctgcggttac ctttcgccag catgatcatg ctgccgccgt gggattgcag cagatccacg
     6361 taggagtcca tacgacctgc ggtggttggg ccaagtgaac ctgatggata accggcaggc
     6421 gttttcgccg gacccgcgta gtagatcggg tgatctttga tgtactgcgg cagttcttta
     6481 ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat cgcgacccac gataatggtg
     6541 ccggtaagcg acaagcgggt ggataccgga tattgcgaaa gctgggcaag gatctctttc
     6601 atcgggcggt taaggtcaac tttcaccgct tcgccctcac cggccttgcg cagttcttgt
     6661 ggaatgtact ggcctgggtt gtgttccagt ttttcgatcc agataccttc gcggttgatt
     6721 ttcgctttaa tgttacggtc agcggagcag gagactccca tgcctaccgg gcaggatgcg
     6781 ccgtgacgtg gcagacggat aacgcggatg tcgtgcgcga agtatttacc gccaaactgc
     6841 gcgccaagac cgagtttctg ggcctcttcc agcagttcct gttccagctg gatatcgcgg
     6901 aacgcctgac catgttcgtt cccttccgtc ggcagttcat cgtaatagtg agcgcttgct
     6961 aacttgacgg ttttcaggtt ggtttccgca gacgtaccgc caatcacaaa cgcgatatgg
     7021 tacggcgggc aggctgcagt accgagggta cgcattttct cgacgaggaa gtttttcagt
     7081 ttgccgggcg tcagcagggc tttggtttcc tggtagagat acgttttgtt ggcagagccg
     7141 ccgcctttag cgacacagag gaatttgtac tcatcgccat ctaccgcgta caggtcgatt
     7201 tgcgcaggca ggttcgtgcc ggtgttgacc tctttataca tgtccagcgg cgcgttctgt
     7261 gaatagcgca ggttatcttc gatataggtg ttatagacgc ctttcgacag cgcttcttca
     7321 tcaccgccac cggtccacac gcgctgacct tttttaccga cgatgatcgc ggtgccggta
     7381 tcctggcagg tcggcag
//
LOCUS       DAWDRC010000133         7396 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4801_length_7396_cov_2.495845, whole genome shotgun sequence.
ACCESSION   DAWDRC010000133 DAWDRC010000000
VERSION     DAWDRC010000133.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4801_length_7396_cov_2.495845"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3917..3926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6228..6237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1803 a         1772 c         1968 g         1833 t
ORIGIN      
        1 aacgagaaac ttgagaatat cgagcgtgag cgtcagcgtc tgaaatcgac ctgggtaacc
       61 ccgtcggcgg aagctgcagc cgaagtgaat gctcacctga ctgcgccgct ttcccgtgaa
      121 gccagtggtg aagatctgct gcgtcgtcct gaaatgactt atgaaaaatt aaccacgctg
      181 acgccgtttg cccctgcgtt gacagatgaa caggctgcgg aacaggttga gattcaggtt
      241 aaatacgaag gttatatcgc gcgccagcaa gatgagatcg aaaagcagct gcgtaacgag
      301 aataccctgc taccagcgac gctggattac cgccaggtat ccggtctttc taacgaagtg
      361 atcgccaaac ttaacgatca caaaccggcc tctattggcc aggcttcgcg tatttctggc
      421 gtcacgcctg cggctatctc cattctgctg gtgtggctga aaaaacaggg tatgctgcgt
      481 cgtagcgcat aacgcattaa aaatgcctgg taagcacccg cttaccaggc aacgcatcaa
      541 gaacaggtaa tcaccgtgct caacaaactc tccttactgc tgaaagacgc aggtatttcg
      601 cttaccgatc accagaaaaa ccagcttatt gcctacgtga atatgctgca taaatggaat
      661 aaagcgtaca acctgacttc ggttcgtgat cctaatgaga tgctggtacg ccatattctc
      721 gatagcattg tggtggcacc gtatctgcaa ggtgaacggt ttatcgatgt cggcaccgga
      781 ccgggactgc ctggcattcc actctctatc gtgcgtcctg aagctcattt cacgttgttg
      841 gatagccttg gtaaacgcgt gcgtttcctt cgtcaggtgc aacatgagct taaactggag
      901 aatattgagc cagtacagag cagggtagaa gagtttccgt cagagccgcc atttgatggc
      961 gtaattagcc gcgcttttgc ctctctgaac gatatggtga gctggtgcca ccatcttcct
     1021 ggtgagcaag gccgtttcta cgcgctgaaa gggcaaatgc cggaagatga aatcgctttg
     1081 ttgcccgagg aatatcaggt cgaatcagtg gttaaacttc aggttccagc cctggatggc
     1141 gaacgtcatc tggtggtgat taaagcaaat aaaatttaat ttttatcaaa aaaatcataa
     1201 aaaattgacc ggttagactg ttaacaacaa ccaggttttc tactgatata actggttaca
     1261 tttaacgcca cgttcactct tttgtatcaa caagataacg tggctttttt tggtaagcag
     1321 aaaataagtc ataagtgaaa atatcagtct gctaaaaatc ggcgctaaga atcatcattg
     1381 actgttaaaa cattattaaa aatgtcaatg ggtggttttt gttatgtaaa tgtcatttat
     1441 taaaacagta tctgttttta gactgaaata tcataaactt gcaaaggcat catttgccaa
     1501 gtaaataaat atgctgtgcg cgaacatgcg caatatgtga tctgaagcac gctttatcac
     1561 cagtgtttac gcgttattta cagtttttca tgatcgaaca gggtttgcag aaaagtcgca
     1621 attgtatgca ctggaaaaat atttaaacat ttattcacct tttggctact tattgtttga
     1681 aatcacgggg gcgcaccgta taatttgacc gctttttgat gcttgactct aagccttaaa
     1741 gaaagtttta tacgacacgc ggcatacctc gaagggagca ggagtgaaaa acgtgatgtc
     1801 tgtgtcgctc gtgagtcgaa acgttgctcg gaagcttctg ctcgttcagt tactggtggt
     1861 gatagcaagt ggattgctgt tcagcctcaa agaccccttc tggggcgtct ctgcaataag
     1921 cgggggcctg gcagtctttc tgcctaacgt tttgtttatg atatttgcct ggcgtcacca
     1981 ggcgcataca ccagcgaaag gccgggtggc ctggacattc gcatttggcg aagctttcaa
     2041 agttctggcg atgttggtgt tactggtggt ggcgttggcg gttttaaagg cggtattctt
     2101 gccgctgatc gttacgtggg ttttggtgct ggtggttcag atactggcac cggctgtaat
     2161 taacaacaaa gggtaaaagg catcatggct tcagaaaata tgacgccgca ggattacata
     2221 ggacaccacc tgaataacct tcagctggac ctgcgtacat tctcgctggt ggatccacat
     2281 aaccccccag ccaccttctg gacaatcaat attgactcca tgttcttctc ggtggtgctg
     2341 ggtctgttgt tcctggtttt attccgtagt gtagccaaaa aggcaaccag cggcgtgcca
     2401 ggtaagtttc agaccgcgat tgagctggtg atcggctttg ttaatggtag cgtgaaagac
     2461 atgtaccatg gcaaaagcaa gctgattgct ccgctggccc tgacgatctt cgtctgggta
     2521 ttcctgatga acctgatgga tttactgcct atcgacctgc tgccgtacat tgctgaacat
     2581 gtactgggtc tgcctgcact gcgtgtggtt ccgtctgctg acgtgaacgt aacgctgtct
     2641 atggcactgg gcgtatttat cctgattctg ttctacagca tcaaaatgaa aggcatcggc
     2701 ggcttcacga aagagttgac gctgcagccg ttcaatcact gggcgttcat tcctgtcaac
     2761 ttaatccttg aaggggtaag cctgctgtcc aaaccagttt cactcggttt gcgactgttc
     2821 ggtaacatgt atgccggtga gctgattttc attctgattg ctggtctgtt gccgtggtgg
     2881 tcacagtgga tcctgaatgt gccgtgggcc attttccaca tcctgatcat tacgctgcaa
     2941 gccttcatct tcatggttct gacgatcgtc tatctgtcga tggcgtctga agaacattaa
     3001 tttaccaaca ctactacgtt ttaactgaaa caaactggag actgtcatgg aaaacctgaa
     3061 tatggatctg ctgtacatgg ctgccgctgt gatgatgggt ctggcggcaa tcggtgctgc
     3121 gatcggtatc ggcatcctcg ggggtaaatt cctggaaggc gcagcgcgtc aacctgatct
     3181 gattcctctg ctgcgtactc agttctttat cgttatgggt ctggtggatg ctatcccgat
     3241 gatcgctgta ggtctgggtc tgtacgtgat gttcgctgtc gcgtagtaag cgttgctttt
     3301 atttaaagag caatatcaga acgttaacta aatagaggca ttgtgctgtg aatcttaacg
     3361 caacaatcct cggccaggcc atcgcgtttg tcctgttcgt tctgttctgc atgaagtacg
     3421 tatggccgcc attaatggca gccatcgaaa aacgtcaaaa agaaattgct gacggccttg
     3481 cttccgcaga acgagcacat aaggaccttg accttgcaaa ggccagcgcg accgaccagc
     3541 tgaaaaaagc gaaagcggaa gcccaggtaa tcatcgagca ggctaacaaa cgccgctcgc
     3601 agattctgga cgaagcgaaa gctgaggcag aacaggaacg tactaaaatc gtggcccagg
     3661 cgcaggcgga aattgaagcc gagcgtaaac gtgcccgtga agagctgcgt aagcaagttg
     3721 ctatcctggc tgttgctggc gccgagaaga tcatcgaacg ttccgtggat gaagctgcta
     3781 acagcgacat cgtggataaa cttgtcgctg aactgtaagg agggaggggc tgatgtctga
     3841 atttattacg gtagctcgcc cctacgccaa agcagctttt gactttgccg tcgaacacca
     3901 aagtgtagaa cgctggnnnn nnnnnngcgc ttgcgccaga aacgctcgcc gagtcgttta
     3961 tcgcagtttg tggtgagcaa ctggacgaaa acggtcagaa cctgattcgg gtaatggctg
     4021 aaaatggtcg tcttaacgcg ctcccggatg ttctggagca gtttattcac ctgcgtgccg
     4081 tgagtgaggc taccgctgag gtagacgtca tttccgctgc cgcactgagt gaacaacagc
     4141 tcgcgaaaat ttctgctgcg atggaaaaac gtctgtcacg caaagttaag ctgaattgca
     4201 aaatcgataa gtctgtaatg gcaggcgtta tcatccgagc gggtgatatg gtcattgatg
     4261 gcagcgtacg cggtcgtctt gagcgccttg cagacgtctt gcagtcttaa ggggactgga
     4321 gcatgcaact gaattccacc gaaatcagcg aactgatcaa gcagcgcatt gctcagttca
     4381 atgttgtgag tgaagctcac aacgaaggta ctattgtttc tgtaagtgac ggtgttatcc
     4441 gcattcacgg cctggccgat tgtatgcagg gtgaaatgat ctccctgccg ggtaaccgtt
     4501 acgctatcgc actgaacctc gagcgcgact ctgtaggtgc ggttgttatg ggtccgtacg
     4561 ctgaccttgc cgaaggcatg aaagttaagt gtactggccg tatcctggaa gttccggttg
     4621 gccgtggcct gctgggccgt gtggttaaca ctctgggtgc accaatcgat ggtaaaggtc
     4681 cgctggatca cgacggcttc tctgctgtag aagcaatcgc tccgggcgtt atcgaacgtc
     4741 agtccgtaga tcagccggta cagaccggtt ataaagccgt tgactccatg atcccaatcg
     4801 gtcgtggtca gcgtgaattg atcatcggtg accgtcagac cggtaaaacc gcactggcta
     4861 tcgatgccat catcaaccag cgcgattccg gtatcaaatg tatctatgtc gctatcggcc
     4921 agaaagcgtc caccatttct aacgtggtac gtaaactgga agagcacggc gcactggcta
     4981 acaccatcgt tgtggtagca accgcgtctg aatccgctgc actgcaatat ctggcaccgt
     5041 acgctggttg cgcaatgggc gaatacttcc gtgaccgcgg tgaagatgcg ctgatcattt
     5101 acgatgacct gtctaaacag gctgttgctt accgtcagat ctccctgctg ctccgtcgtc
     5161 cgccaggacg tgaagcattc ccgggcgacg tattctacct ccactctcgt ctgctggagc
     5221 gtgctgcgcg tgttaacgcc gaatacgttg aagccttcac caaaggtgaa gtgaaaggga
     5281 aaaccggttc tctgaccgcg ctgccgatta tcgaaactca ggcgggtgac gtttctgcgt
     5341 tcgttccgac caacgtaatc tccattaccg atggtcagat cttcctggaa accaacctgt
     5401 tcaacgccgg tattcgtcct gcggttaacc cgggtatttc cgtatcccgt gttggtggtg
     5461 cagcacagac caagatcatg aaaaaactgt ccggtggtat ccgtaccgct ctggcacagt
     5521 atcgtgaact ggcagcgttc tctcagtttg catccgacct tgacgatgca acacgtaagc
     5581 agcttgacca cggtcagaaa gtgaccgaac tgctgaaaca gaaacagtat gcgccgatgt
     5641 ccgtagcgca gcagtctctg gttctgttcg cagcagaacg tggttacctg gcggatgttg
     5701 aactgtcgaa aatcggcagc ttcgaagccg ctctgctggc ttacgtcgac cgtgatcacg
     5761 ctccgttgat gcaagagatc aaccagaccg gtggctacaa cgacgaaatc gaaggcaagc
     5821 tgaaaggcat cctcgattcc ttcaaagcaa cccaatcctg gtaacgtctg gcggcttgcc
     5881 ttagggcagg ccgcaaggca ttgaggagaa gctcatggcc ggcgcaaaag agatacgtag
     5941 taagatcgca agcgtccaga acacgcaaaa gatcactaaa gcgatggaga tggtcgccgc
     6001 ttccaaaatg cgtaaatcgc aggatcgcat ggcggccagc cgtccttatg cagaaaccat
     6061 gcgcaaagtg attggtcacc ttgcacacgg taatctggaa tataagcacc cttacctgga
     6121 agaccgcgac gttaaacgcg tgggctacct ggtggtgtcg accgaccgtg gtttgtgcgg
     6181 tggtttgaac attaacctgt tcaaaaaact gctggcggaa atgaagannn nnnnnnnacc
     6241 tggaccgaca aaggcgttca atgcgacctc gcaatgatcg gctcgaaagg cgtgtcgttc
     6301 ttcaactccg tgggcggcaa tgttgttgcc caggtcaccg gcatggggga taacccttcc
     6361 ctgtccgaac tgatcggtcc ggtaaaagtg atgttgcagg cctacgacga aggccgtctg
     6421 gacaagcttt acattgtcag caacaaattt attaacacca tgtctcaggt tccgaccatc
     6481 agccagctgc tgccgttacc ggcatcagat gatgatgatc tgaaacataa atcctgggat
     6541 tacctgtacg aacccgatcc gaaggcgttg ctggataccc tgctgcgtcg ttatgtcgaa
     6601 tctcaggttt atcagggcgt ggttgaaaac ctggccagcg agcaggccgc ccgtatggtg
     6661 gcgatgaaag ccgcgaccga caatggcggc agcctgatta aagagctgca gttggtatac
     6721 aacaaagctc gtcaggccag cattactcag gaactcaccg agatcgtctc gggggccgcc
     6781 gcggtttaaa caggttattt cgtagaggat ttaagatggc tactggaaag attgtccagg
     6841 taatcggcgc cgtagttgac gtcgaattcc ctcaggatgc cgtaccgcgc gtgtacgatg
     6901 ctcttgaggt gcaaaatggt aatgagcgtc tggtgctgga agttcagcag cagctcggcg
     6961 gcggtatcgt gcgtaccatc gcaatgggtt cctccgacgg tctgcgtcgc ggtctggatg
     7021 taaaagacct cgaacacccg atcgaagtcc cggtaggtaa agcgactctg ggccgtatca
     7081 tgaacgtact gggtgaaccg gtcgacatga aaggcgagat cggtgaagaa gagcgttggg
     7141 cgattcaccg cgcagcacct tcctacgaag agctgtcaaa ctctcaggaa ctgctggaaa
     7201 ccggtatcaa agttatcgac ctgatgtgtc cgttcgctaa gggcggtaaa gttggtctgt
     7261 tcggtggtgc gggtgtaggt aaaaccgtaa acatgatgga gcttattcgt aacatcgcga
     7321 tcgagcactc cggttactct gtgtttgcgg gcgtaggtga acgtactcgt gagggtaacg
     7381 acttctacca cgaaat
//
LOCUS       DAWDRC010000134         7394 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4804_length_7394_cov_2.203570, whole genome shotgun sequence.
ACCESSION   DAWDRC010000134 DAWDRC010000000
VERSION     DAWDRC010000134.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4804_length_7394_cov_2.203570"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    248..257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1121..1130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3615..3624
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4706..4715
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6343..6352
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1881 a         1904 c         1885 g         1674 t
ORIGIN      
        1 ctcacccaga aaggcgtcgg aagaactgaa ggtaaaagta ccggtcgaac gctgacgacc
       61 gctccttccg agatctgttt cagagggatt gaggcaacac tgcatccgca cgccagtgcc
      121 agagtcgccg aagctagggc gcaggcgctc gtaggatggg atgatgccag aaaagcgtgg
      181 cttgaacagg ttgccatcac ggagcaaggc tcaggcttgg gcagcagtcg tgcgctggat
      241 gccgatgnnn nnnnnnnttc tgcgctgaaa acagtgttaa aacagccgct tggcgcagaa
      301 attgaacgtg cggcgctctc cgccaaacag gaacttgcca gcacgttaaa aaatgttcag
      361 ctctttgcca acatcaaaga tagtgacgat ttcctcagac aactgaacga ctggcagacg
      421 tttattgaga ctgtgcgggc cgagcactac cccactggca gcgctcagta caagccatct
      481 tcgtggctgc tgggggaaat tgaagctctc agaaccaact ccgaggagag tctggcgaca
      541 ttaaatgcgt tactttccat tgaaaaacag aaagatagtg cgaaattgtg gctggagatc
      601 tgtcaacttg atggtgacta cgtttcaaga atcagcagca tacttgcgca atggcaactg
      661 gtacaaccca agatcgcgtt cacactccag cagatgaatg ccaaagggga tatccagcaa
      721 ctgactcttg ctctgaataa agtgaatgac cgtctaaaaa cacttgcgag cgatatcatc
      781 gcctctggag ttgatcaatg accaccttac gccaacaact ggccgagatg caaaaagaac
      841 tcgatcttag ctttcaggct ggtaaggcct cacaaatcat cgataaatgt aatctggtgt
      901 gcagcaaaac taacaccatt gcagataagt ttgtattgct taaacagcaa tattcagccc
      961 agcaacaact cccgcggggc attgcggtta cctggaatga cgagcagcgt agcgaactga
     1021 tcgctgccgc gaacagcgtc aaaagtctca tgttgcagtg gagacgctgg ctgaaaaaac
     1081 agtcggtaga caattctggc gaggaggaga accttgcaga nnnnnnnnnn cagatcagga
     1141 agagtcacca caggaatctc tgggtgagac tctgaccgcc tcagaggaac ctactgccta
     1201 cgacatcatc cagaatgatt ccttgaccca ttgcgtcaac caactcggcg ccctgcacaa
     1261 ggacctaagc aaagaactaa aagatgcttg gaatacatgg ctggaagagt tacatcatca
     1321 agttcacgtc gatgacaaaa ctctgaattt tcagaaaaat acgaataaat ttgatgatat
     1381 cgcctccgac tacgagcgtc gacgtctgga tttcgaaaac ttaacgcaga gccagccagt
     1441 cagcgatgaa aagattcacc aaattaaagc gctagccgag aaactgtatt ccctgcgcgg
     1501 catgatgaaa acggactggc ctgacgtggt tcaggcattt ttcaatcgca ttaatggccc
     1561 gcagaacttg cgcccgacgt taagcgccct tacgcctgag gtgcttgagt ggttaacggt
     1621 tgaagagatg ctggatgagt tcgtgatcac aaggaaataa tttatgtcta cggctgattt
     1681 gtccctcctg caccaagcgc tggatctgct cgaagagcgg gaagcagaac tgctggtctg
     1741 gggggataca gatggcgcat ttacccatgc cgatatcatc tcgctcttta cccggcacct
     1801 tccgtacgaa gatgccgagt cgttactggc agaactgcag aaggcagcca tgcttttcaa
     1861 cgtcccgacc agcaaagggg gaactcgcta ccgcacgcgt atggcagaag cggtatgcct
     1921 gttccgccac cagcgtcaat ggttccgcaa tcaaccgctt cagcaagccc ggacgcttgt
     1981 cgccgactac cgtttcgtcc gtcgcccgcg caactatccg aagcgtgaac ttccggcgca
     2041 aacggtgctg agcaaatggg tcgatgttcc gtggatgaac gaaattaaac gccaggcgct
     2101 ttcccgactg atcggcgact ttttcattgc cggctttcag gaacgctcaa ccgaacgtat
     2161 tttgcgcgcc tggcactttc atagtcatca tgcccgcgtg aaagaagcta ccgggaccat
     2221 tgtctgtgcc ggtacgggga gtggtaaaac actggcgttc tatctcccgg cactcacctc
     2281 gctcctcaat gatattcagc gcgacaatgc gcaacgggtc aggacgctgg cactgtatcc
     2341 acgtaaagaa ctgcttaaag atcagttcat ggaaacctgg agtaaatgtc gtgaacttga
     2401 taatcaggcc ctagtactga caggacgcaa gatccgtatt ggttcttttt tcggtgatac
     2461 cccgttcaac catcaatacg caatgaaaga taaagacaag gatatgccct ttgatttact
     2521 ccgttgtacc acgccgaaat gttcggggca aatgcactgg aaagcagaag atatcaaagc
     2581 gaaaaaagag attcttcgtt gtagccactg taatcacagc gtagacagtg acgaagtaat
     2641 cctgacccgc gtctcgctga tgaaaaaccc gcctgacatc ctgtttacca ctaccgaaat
     2701 gctcaatcag cacctaggga ataaccaaac gaatcaccta tttggcgttg gcattgacgt
     2761 aacacctccg ccggtagtcc tgctggatga agtacacacc tatgtgggga ataccggtgc
     2821 acagacggcc tatctactgc gacgctggat gcaacttgcg cgcagccacc ctcattttgt
     2881 tgggctttcg gcaacgctga gtgacgccga acgtttcttc gccgatctgg taggtgcaca
     2941 taaaaaacat gtggctctga tcgaaccgaa gttccacgaa atggaagatg aaggggctga
     3001 atatctgctg gcattacgcg gtgatccggt ctcgcaaacc gccttgctat ctactactat
     3061 ccagaccagc atgctcgctc ggcgtattct ggatacgaaa aggaaaaaat catgcgggac
     3121 atgggggcaa aaaacctttg tcttcaccga cgatctggat atcaataacc gactttatca
     3181 tcaactgtgt gacgccgaag gctggcggtc gcgtggtcaa aacttatacg ttgcaaaacc
     3241 accgctggct tctcagcgtc tggaaacacc gaaaaacagc cataagctga cactggcagg
     3301 acagaactgg cgtatcgcgc tggatattgg tcactcactt gacgaaaatg accgagccat
     3361 cgttgcccgc acgtccagtc aggacgtcgg ggtcgatcct agagcggaag tggtgattgc
     3421 aaccgcgtcg ctggaggtgg gctttaacga cccctccgtt ggcatggtta ttcagcataa
     3481 agcgccacgt aacgttgcct cctatttgca gcgcaaaggg cgtgctggtc gttcaagaac
     3541 catgcgtccg tggatggtcg ctgtcctatc cgaatttggc cgcgaccgag tcgtttatca
     3601 acgctatgaa gagcnnnnnn nnnnaatggg caacatccat atccagaaaa tgcaggcggc
     3661 aatggcgaca cttgactggc tgagcatgaa gatccccgga agtaatatct ggtcattgct
     3721 gaataaaccg cagacaaagc gagaatctct tgatcatctt gatcgtatgc tccacctgat
     3781 tactgccgtg attgaacaac atgcgtggca gctggagctg gagaagtatc tgttttacgc
     3841 gttgcgcatc actgacgagc aacttcagcg tgtactgtgg tcgccgcccc gttcgatcat
     3901 gatggagttg ctgccgacat taaaacgaca actcacgacc caatggtcgc ggatgggcca
     3961 accggaggca gatcgttcga cgggacgttc cccactgccg gcctttattc cttcagcgct
     4021 attcagtgag cttaatcttc cggaactgga gatccatctc gagcggaagt tcgagaagcg
     4081 tgaacatttg ccctttattc aggggctgaa agagtttgcg ccggggcgca tttccaagcg
     4141 ctatgccgtt tactccgaca atgaagcaga ctggcttatc ccaaacaact ttgttccggc
     4201 gatgaattgc gtttcggatg ttgattttga aattgagcag gctttcggca atacttggca
     4261 gcccgagtgt gtcatcgagt gcgaaggtca gccccctatt agggtcattc gcccgatgca
     4321 ggtgcttacg cgcgcgctga gatttgatca aaatctcacc gagaaaagca actcgcaact
     4381 gtactggcat acccgtttta acccagacga tcaggccgtt ccgctcccca ttcctaccgg
     4441 cccgtgggcg gagatcctcc gctccattac gttcttcacc caccagaaca tgacacctct
     4501 agatgtcacg cgttacgcca cccacgcaac agcctcatta cgcaataaaa ccaagcaaca
     4561 ggcgcatgtg aacttcagat gggtcgagca tggcgagcct gtcggggttg gcgtgcggca
     4621 gtgggtggat ggcgcacggc tcagatttac gctgccaccc aatctgctgc aacgcctccg
     4681 gcatgacccg gccattcagc gcgcannnnn nnnnnacact cttccaggca gataatatct
     4741 ttgaaaattg cgaggatggt gaagacgacg ttgaaagtca ggaactgcag caaaatttgc
     4801 gcaacctgtt taaagagacc gacattctgg acatgataga taggcacagc gcgatcctga
     4861 ctcaggatct cagtgatgat gtcgattttc aggactggct acaggagtta ctgacaacga
     4921 cgctggccgg agcggtcaaa caaaccagcc acctgttgct gccggatgtc gatgaacgcg
     4981 gtctggtggt ggataccgaa ctaaacgacg aagtactgga cgtctggctg accgaatcag
     5041 aacccggcgg ctgtggtatc attacccgta tggaagacgt ttttcatcag gatccggtct
     5101 ccgtactgaa tctgtttatg cgtagttttg ccgtcagcga ttacgagcag atcgactaca
     5161 acctgttcga gatgctttcg cggctctcat cttccagcga acttcaggag gccttgaatg
     5221 cgatacgtca ggccagcagt catctgcagc gtcgccaggc taacgcccac ctcagagcgc
     5281 tgctaaaggc gcagggattt gcacttagtc acagttttat gtcggtgctg cacacgcggg
     5341 tattacgtcc gggcagccaa gcgtcacatg atgcgcagat gcttgcatat ctcaacgcat
     5401 ggcgggaatt ggaagataaa gccggatacg aaatcgctct gaatattttt gcccataccc
     5461 aagcgacgca agaactgcct gacgccagtg tgattaaagt ttttgagcgc ttttgcaaaa
     5521 ttcagggaat gctctggcag cgcggcaatg ccatccgtcg gtcggttctc agctattaca
     5581 atccgtttaa gtccggtaac aacctgactg aacgactgct gctaagctca ctgtttcaac
     5641 agacggcgtg tagcatcagc atcagcgaga gcgattggtt agcgcagtta catcacgcca
     5701 ttacccaaca cggctttgca gaactgcata tcccgcgcga ggctcgccat cgtattgtgg
     5761 aagtgatctc tctggtgcag gtgactccaa ttgagtactt tggtttgcat ctttatcccc
     5821 gcgaatccgc cgtggattat caggatggca atctggtgtt acgttttgaa ctggccgaag
     5881 cgctactgta aggaacgcag aaatggaaga acaacgacaa attttcctgc atggcccact
     5941 gggccagcga catttgcggg aaatcctaag cgcgcagttt agtggcttga tcctctatcc
     6001 ggaactgatc tggctgatct ctccgtggat gagtgacttt gacgtgattg ataaccgagg
     6061 cggtcagtgg agttttcttg atccttcatg gggagctcgg atgatcagct ttcaggaatt
     6121 gctcgccacc gccgttaaca atggttgccc gttacgtatc gtgacacgta aagacacgca
     6181 cagcgtggtc tttatcgagc gactattgat gcgcttatcg cccgatcacg acatgcaata
     6241 cctatatcac gagaacctgc acgctaaggg attgctgacc aaacatttct ttctgaaagg
     6301 ctccatgaat tacacttgga gcggcgcaaa cgtgaacgat gannnnnnnn nnatgagcat
     6361 ctgttgtttt caaccaataa tacggtgatt tcagatgcgt tgattgaatt tagcggccag
     6421 tacacctttg gagacaccca tgactaatgt tcctcaatgg ctgcgtgact tctttggcaa
     6481 cggaaattta ctgaagctgg acaggttgct ggacaacgtt gaaaacgcct accctgcaga
     6541 cttaaaagcc gtattactgc cgctttacga gagcgcgact gatgctcagt ggccactcat
     6601 tctgccctgg tgtgatgctc atcgctgggt attttttgcc gcggcagata cagctcgcac
     6661 cacgcaggaa ctcagcaatg tgttgaatgc caggctgggg tctgcagatg ttattgctga
     6721 cagaaaggtg acctttgtcc ccgcacaggg agcgacgtcc ctgtctgaaa cggcgttgct
     6781 gacgcactgt ccagccggat ttatccggat cgaattattg cccacaaagc ttaaagataa
     6841 accagcaaaa gagcgggttt tcgccgcgct caaagacgtt atcgtcctat ttcgtgacag
     6901 gccatcaatc gtacgcacgg tcaaacgccc cttcgggcga atcttgagtg atttcattct
     6961 ggcgaatagc caaaaagacg aagccacaag cgacgctgta ctaaaggaag agaagaacaa
     7021 cggtacgctt tcgcgacgta accttatgct gctcgaactc cagcaggcgg gtaagttaga
     7081 gaagtgggac acactgctga atcacgattc tcttgctgat ttggttcggg gacgcattcc
     7141 tacaaccctg atgcggatgc tgctgaaagc ataccagcag cgctatttta cacccgacag
     7201 ccatgggtat ccgcaagcct caccggcgga tcttcgcccg caatgtctgg cactaaaccc
     7261 tctgtttacg cagatacctt ttttatccca agatgaaact gactttgcgg cgtggaaaac
     7321 ttgggccact ggagtgatgc ttattggtga agtcgacctg ctcaacgcgt tgccagaacg
     7381 cctaaaaacc gact
//
LOCUS       DAWDRC010000135         7384 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4814_length_7384_cov_2.369218, whole genome shotgun sequence.
ACCESSION   DAWDRC010000135 DAWDRC010000000
VERSION     DAWDRC010000135.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4814_length_7384_cov_2.369218"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5481..5490
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1927 a         1638 c         1783 g         2026 t
ORIGIN      
        1 tttggccgct cgggaacccc accacggggt aatgcttttt actggcctgc tcccttatcg
       61 ggaagcgggg cgcatcatat caaatgacgc gccgctgtaa agtgttacgt tgagaaaaat
      121 gaactggttg cgtaattttc atccgtaacg gattaaagct aaccagttat ttttgctggc
      181 gattaaagaa taatcgtccg attaccgtaa acaaagacgc gctgcgccag tactttgtat
      241 agcgcacgac ttaagacgtt tttctcgacg tcacgacctg cgcgcatcat atcttcagct
      301 gtgtaggtat gatcgacatg aataacgtcc tgcatgatga ttgggccttc gtccagattg
      361 tcattcacat agtgagcggt tgcgccaata atcttcacgc cacgctcata ggcctggtga
      421 taaggacgtg cgccgataaa cgctggcagg aatgaatggt gaatattgat gatcttattc
      481 gggaagcgtg ccacaaattc cggcgttaat acccgcatat acttcgccag caccacatag
      541 tcaggttgat aagcatcaat ggcatccgcc atcttttgat cgtgctcgtt gcggcttaac
      601 ccttcatggc ttaccagctc aaacggaata tcaaaccgct caaccagaga acgtaaagta
      661 tcgtggttac caataactgc cgcgatttcg acatccaggc cgccgtaatt ggctttcatc
      721 aacaaatcgc caaggcaatg cgcttcttta gtgaccagaa tcacgatccg gcgacgaccg
      781 gcaggattca gctcgcgcac ggagccttct ggcaatgcgc tatcgagatc cgccagcagg
      841 gtggaatcat taaaaatccc ttccagttcc gtgcgcataa aaaagcgccc ggtacgatga
      901 tctacaaatt cattgttctg cacgatattt aactcgtgct tgtagcaaat attggtaata
      961 cgtgcgatca gacctttttg gtccggacaa atagtacgca gaactttacg ttggagtgaa
     1021 tgcattgctg gaaaaacctt gttgagagtg tttgctaaac cgtaccgtca accattattg
     1081 gccgcagcac tttttaaatt ttttacctga accacaaggg cagggatcgt tgcggccaaa
     1141 ctgcggacgt gtaccgtcaa tatagttcca ttgaccgttt tctttcaaaa atcgcgaacg
     1201 ttcaattatt gcacctgttt tgccaccctc ggtaaatcga gcaacaaaac tgacaaatcc
     1261 gatattgccg ccgtcctgcc agcaatgttc aaatacggtc aatcccaacc attcagtatg
     1321 cgcaaatccg gcaatcaatt cggctcgtaa cgccgctgct ccacaagatg gatgccaggt
     1381 ctttattaaa taatctgcgt cttgcatcac aaaagcgcag taacgcgaac gcatgagatg
     1441 ttctggatca ggtgcaacct tttcaccaga cacataaggg tggcaacata ggctatactc
     1501 gacagcacta ccacagggac aaagctgaga cacaaataat ctccctggaa acaataacgg
     1561 cgtattaacc gcctgagtag cactatgtta accgagcagt agcgatgtgg ctacgattgc
     1621 attccagggg aatcttgcgg gaataatgag aaagataaaa atagggctgg cgctgggatc
     1681 tggcgcggca agaggttggt cgcatattgg cgtaattaat gcgctaaaaa aagtgggtat
     1741 tgaaattgat atcgttgcag gatgttcaat tggttcgctg gtgggcgctg cctatgcatg
     1801 cgatcgatta tctgcgctgg aagattgggt gacctctttc agttattggg atgttttacg
     1861 cctgatggat ctctcctggc agcgcggtgg gttactgcgc ggcgagcgtg tcttcaatca
     1921 atatcgcgaa ataatgccgg aaacagagat cgaaaattgt tcccgtcgct tcgcggctgt
     1981 tgccaccaat ttaagtactg ggcgtgaatt atggtttact gaaggcgatc tccatcttgc
     2041 tattcgtgca tcatgcagta ttccaggact aatggcaccc gttgcgcata acggctactg
     2101 gctggttgat ggcgctgtag ttaacccaat tcctatttcc ctcacgcgtg cattgggggc
     2161 tgatattgtg atagcggtcg acctgcagca cgatgctcat ttgatgcaac aagatttgct
     2221 ctcctttaat gtcagtgaag aaaatagcga gaatggtgat tctctgccgt ggcatgcgcg
     2281 tctgaaagaa aggttaggca gcataacgac acgtcgggcg gtgacagcgc caacggcaac
     2341 agagattatg accacttcta tccaggtgct ggagaaccgc cttaaaagga accgcatggc
     2401 aggtgatccg cccgatattc tgattcaacc tgtttgcccg caaatatcta cgcttgattt
     2461 ccatcgcgcg cacgctgcca ttgcggccgg acaactggca gtggaaaaga aaatggacga
     2521 acttttgccg ttggtacgca ccaacatttg accagaattt ttatctacac ttaagttaat
     2581 tctgacaggc gtaggtggca atagcgtgcc actattgagt aaagccagtc aggggagaga
     2641 acatgacgca gccattggtc ggaaaacaga ttctcattgt tgaagatgag caggtatttc
     2701 gctcgcttct ggattcgtgg ttttcctcat tgggagcgac aacggtactg gcggctgatg
     2761 gggtggatgc ccttgagttg ctgggaggtt tcactccaga cctgatgata tgtgatatcg
     2821 cgatgccacg aatgaacggg cttaaactgc tggaacatat acgcaacaga ggtgaccaga
     2881 caccagttct ggtgatatct gccactgaaa atatggcaga tattgccaaa gcgttacgtc
     2941 tgggtgttga agatgttttg ctgaaaccgg ttaaagatct gaatcgcttg cgcgagatgg
     3001 tttttgcctg tctctatccc agcatgttta attcccgcgt tgaggaagag gaaaggcttt
     3061 ttcgcgactg ggatgcaatg gttgataacc ctgctgcagc ggcgaaatta ttacaggaac
     3121 tacaaccgcc ggttcagcag gtgatttccc aatgccgggt taattatcgt caattagttg
     3181 ccgcggacaa acccggcctg gtgcttgata ttgccgcact ttcggaaaac gatctggcat
     3241 tttattgcct tgatgtcacc cgagctggac ataatggcgt acttgctgcc ttgttattac
     3301 gcgcattgtt taacggatta ttacaggaac agcttgcaca ccaaaatcaa cggttgccag
     3361 agttgggcgc gttattgaag caggtaaacc atttactccg tcaggccaat ctgccggggc
     3421 agtttccgct attagttggc tattatcatc gcgaactgaa aaatctcatt ctggtttctg
     3481 cgggtctgaa tgcgacgtta aataccggcg aacatcaggt gcaaatcagt aatggtgttc
     3541 cgttaggcac tttaggtaac gcttatttga atcaattgag ccagcgatgc gatgcctggc
     3601 aatgccaaat atggggaacc ggtggtcggc tgcgcttgat gttgtctgca gaatgagcaa
     3661 acgataacac gggctaaatt tgcattacct gctaatgtca gctggtggta ctatcgtcgc
     3721 cattcgtata agtaattgtc ttaattatgc taactcgcct ccttttcaga acttagcccc
     3781 ttcggggtgc tgatatactg ggatgcgata cagaaatatg aacacgttca aaacacgaac
     3841 agtccaggag aatttaaatg gctgccatta atacgaaagt caaaaaagcc gttatccccg
     3901 tagcgggatt aggaaccagg atgttgccgg cgacgaaagc catcccgaaa gagatgctgc
     3961 cacttgtcga taagccatta attcaatacg tcgtgaatga atgtattgcg gctggcatta
     4021 ctgaaattgt gctggttact cactcatcta aaaactctat tgaaaaccac tttgatacca
     4081 gttttgaact ggaagcaatg ctggaaaaac gtgtaaaacg tcaactgctt gatgaagtgc
     4141 agtctatttg tccgccgcac gtgactatta tgcaagttcg tcaggggctg gcgaaaggcc
     4201 tgggacacgc ggtattgtgt gctcacccgg tagtgggtga tgaacctgtg gctgttattt
     4261 tgcctgacgt tattctggat gaatatgaat ccgatttatc acaggataac ctggcagaga
     4321 tgatccgccg ctttgatgaa acgggtcata gccagatcat ggttgaaccg gttgctgatg
     4381 tgaccgcata tggcgttgtg gattgcaaag gcgttgaatt agcgccgggt gaaagcgtac
     4441 cgatggttgg cgtggttgaa aaaccaaaag cggatgttgc gccgtctaat ctcgctattg
     4501 tgggtcgtta cgtacttagc gcggatattt ggccgttgct ggcaaaaacc cctcctggag
     4561 ctggtgatga aattcagctc accgacgcaa ttgatatgct gatcgaaaaa gaaacggttg
     4621 aagcctatca tatgaaaggg aagagccatg actgcggtaa taaattaggt tacatgcagg
     4681 ccttcgttga atacggtatt cgtcataaca cccttggcac ggaatttaaa gcctggcttg
     4741 aagaagagat gggcattaag aagtaacatc cgtatcggtg ttttccacga aacggtgttg
     4801 agcaatcgac gccgtttttt tatagcttat tcttattata ttgtcttaaa ccggacaata
     4861 aaaaatcccg ccgatggcgg gatttttagc atgtgcaatc tacaaaagat tattgcttga
     4921 tcaggaaatc gtcgagggat ttaccttgct catccattgc ttttttgatt acagctggag
     4981 tacggccctg gccagtccag gttttagttt cgccgttttc gtcaacgtag ctatattttg
     5041 ccggacgctg agcacgctta gctttggtgc cagatttaac ggcagcaagg ctattcagca
     5101 gttcgttcgg gtcaataccg tcagcgatca gcatttcgcg atattgctgc agtttacgag
     5161 tgcgctcttc aacttcagca gcagccgcgc tttcttcttc gcgacgttcg ttaacaacaa
     5221 cttctaattt ttccagcatt tcttccagcg tttcaagtgt acattctctt gcctgcgcac
     5281 gaagagtacg gatgttgttc agaattttaa gtgcttcgct cattgtagta atctcaaact
     5341 tatattgggg tggtttgttg aggtaataat agagccttaa attcagttgt gcaatagcca
     5401 ggaatgtaag gaattcaaaa ttgttcttta ttttgtgccg ccaataaata tcttttcata
     5461 aaattagcca gaaaagacgc nnnnnnnnnn ctatttgcac cgatgatttc gcagcacgtg
     5521 aggttaaaac ttcctgattc atgtcacatt ttatggggag ataatcgtag gctgacgacc
     5581 tttcagtctt ctgtattagt cgtgtttacg agaattccct attaagcgaa tgatgaaaag
     5641 tagaacagtc gcaataagag cagggactta gtattgcact atctcctgga ggtcaacaga
     5701 gggctattac atgcgcaaca ggttaaagat tgtgaatagt taccggcagt catttacccg
     5761 cttataacaa gcgaggcagt tgtaatgata gctcagaagg attatgcaag gcttcgtaag
     5821 ggagaacgca tataccactt ctgtgcatac tgttgagctg aaaaactgac gaattatgat
     5881 aaactccagc caactttatt tcatatcatt gagggcctgt ggctgatggc acagctatat
     5941 ttctactatt ccgcaatgaa tgcgggtaag tctacagcat tgttgcaatc ttcatacaat
     6001 taccaggaac gcggcatgcg cactgtcgta tatacggcag aaattgatga tcgctttggt
     6061 gccgggaaag tcagttcgcg tataggtttg tcatcgcctg caaaattatt taaccaaaat
     6121 tcatcattat ttgatgagat tcgtgcggaa catgaacagc aggcaattca ttgcgtactg
     6181 gttgatgaat gccagttttt aaccagacaa caagtatatg aattatcgga ggttgtcgat
     6241 caactcgata tacccgtact ttgttatggt ttacgtaccg attttcgagg tgaattattt
     6301 attggcagtc aatacttact ggcatggtcc gacaaactgg ttgaattaaa aaccatctgt
     6361 ttttgtggcc gtaaagcaag catggtgctg cgtcttgatc aagcaggcag accttataac
     6421 aaaggtgagc aggtggttat tggtggtaat gaacgctacg tttctgtatg ccgtaaacac
     6481 tataaagagg cgttagaagt cggctcatta acggctattc aggaaaggca tcgccacgat
     6541 taataagaat ttctttactg acaggttgtg cagggcgctt ttatcctgtc agttcgtttt
     6601 acgcacttct tccgggctat acacccttct cggcagtttt ttaacgccgc tatacgcctc
     6661 acagagcgct tatacaccga cattgacttg taacctgtaa agtacaatat cgctgtgtgt
     6721 aggcgttata catcgtcgca aatatgatga aggacaatgc tgccggttta tggaaaagtt
     6781 gttttgggaa aacaaaaaac ggccccagaa ggggtaatgc cagtcagtta agcaactgac
     6841 tggctctttt tcggggctgt ggggtatttc cagggcctct cctttaccac tctcgggaag
     6901 gccctttccc ttcttgtcgg taatttcaca agttgtccca tacttgcaag atcgcgcatc
     6961 agctccggta tacgtcccgg tgaagcgccc tgcaatgtca tcagcattct catcaccatt
     7021 ccgcatgatt ctgagaaact cagttgattc ggccagtaac ctttcagatg ttccgccatt
     7081 ttaatcatct gatatctcac cagattataa gccagtaaga caccccacag ctcttgctcc
     7141 acaagctccg gctttttact tctcagcgtc agcctgctca gttgcatcgt ctgttttatc
     7201 tctctgtatc cctggtcagg ttgacggggc actagtggtt tcagcggtta atcgtcgaac
     7261 aggcgcgaga caatattcgc attcaacaaa ataacaaacg caacactgat cgttttgttg
     7321 agaggataac tctttttcag ggttatgggt gaaaggcctg aaaatttgcg attatcttta
     7381 agag
//
LOCUS       DAWDRC010000136         7298 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4878_length_7298_cov_2.269916, whole genome shotgun sequence.
ACCESSION   DAWDRC010000136 DAWDRC010000000
VERSION     DAWDRC010000136.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7298)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4878_length_7298_cov_2.269916"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1887..1896
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3357..3366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1735 a         1724 c         1962 g         1857 t
ORIGIN      
        1 gcaggagaag ggctttagtg ttaactttga gcgccttttg gccgagatca aagaacgcga
       61 cgaccgcgat cgtaaccgag cggtagcgcc actggttccg gcagccgatg ctttagtgtt
      121 ggattccacc accttaagca ttgagcaagt gattgaaaaa gcgctacaat acgcgcgcca
      181 gaaattggct ctcgcataag cgaccgaatt tgcagtaccc ccgttgcaat ggaatgacag
      241 cgggtatgtt aaacaacccc atccggcatg gagccaggtg gacgttaaat ataaacctga
      301 agattaaaca tgactgaatc ttttgctcaa ctctttgaag agtccttaaa agaaatcgaa
      361 acccgcccgg gttctatcgt tcgtggcgtt gttgttgcta tcgacaaaga cgtagtactg
      421 gttgacgctg gtctgaaatc tgagtccgcc atcccggctg agcagttcaa aaacgcccag
      481 ggcgagctgg aaatccaggt aggtgacgaa gttgacgttg ctctggacgc agtagaagac
      541 ggcttcggtg aaactctgct gtcccgtgag aaagctaaac gtcacgaagc ctggatcacg
      601 ctggaaaaag cttacgaaga tgctgaaact gttaccggtg ttatcaacgg caaagttaag
      661 ggcggcttca ctgttgagct gaacggtatt cgtgcgttcc tgccaggttc tctggtagac
      721 gttcgtccgg tgcgtgacac tctgcacctg gaaggcaaag agcttgaatt caaagtaatc
      781 aagctggatc agaagcgcaa caacgttgtt gtttctcgtc gtgccgttat cgaatccgaa
      841 aacagcgcag agcgcgatca gctgctggaa aacctgcagg aaggcatgga agttaaaggt
      901 atcgttaaga acctcactga ctacggtgca ttcgttgatc tgggcggcgt tgacggcctg
      961 ctgcacatca ccgacatggc ctggaaacgc gttaagcatc cgagcgaaat cgtcaacgtg
     1021 ggcgacgaaa tcactgttaa agtgctgaag ttcgaccgcg aacgtacccg tgtatccctg
     1081 ggcctgaaac agctgggcga agatccgtgg gtagctatcg ctaaacgtta tccggaaggt
     1141 accaaactga ccggtcgcgt gaccaacctg accgactacg gctgcttcgt tgaaatcgaa
     1201 gaaggcgttg aaggcctggt acacgtttcc gaaatggatt ggaccaacaa aaacatccac
     1261 ccgtccaaag ttgttaacgt tggcgatgta gtggaagtta tggttctgga tatcgacgaa
     1321 gaacgtcgtc gtatctccct gggtctgaaa cagtgcaaag ctaacccgtg gcagcagttc
     1381 gcggaaaccc acaacaaggg cgaccgtgtt gaaggtaaaa tcaagtctat cactgacttc
     1441 ggtatcttca tcggcctgga cggcggcatc gacggcctgg ttcacctgtc tgacatctcc
     1501 tggaacgttg caggcgaaga agcagttcgt gaatacaaaa aaggcgacga aatcgctgca
     1561 gttgttctgc aggttgacgc agaacgtgaa cgtatctccc tgggcgttaa acagctcgca
     1621 gaagatccgt tcaacaactg ggttgctctg aacaagaaag gcgctatcgt aaccggtaaa
     1681 gtaactgcag ttgacgctaa aggcgcaacc gtagaactgg ctgacggcgt tgaaggttac
     1741 ctgcgtgctt ctgaagcatc ccgtgaccgc gttgaagacg ctaccctggt tctgagcgtt
     1801 ggcgacgaag ttgaagctaa attcaccggc gttgatcgta aaaaccgcgc aatcagcctg
     1861 tctgttcgtg cgaaagacga agctgannnn nnnnnntctc caacaacgca atggctgaag
     1921 ctttcaaagc agctaaaggc gagtaattct ctgactcttc gggattttta ttccgaagtt
     1981 tgttgagttt acttgacaga ttgcaggttt cgtcctgtaa tcaagcacta agggcggcta
     2041 cggccgccct taatcaatgc agcaacagca gccgcttaat ttgcctttaa ggaaccggag
     2101 gaatcatgac caagtcagaa ttgatagaaa gacttgccac ccagcaatcg cacattcccg
     2161 ccaagacggt tgaagatgca gtaaaagaga tgctggagca tatggcctcg actcttgcgc
     2221 agggcgagcg tattgaaatc cgcggtttcg gcagtttctc tttgcactac cgcgcaccac
     2281 gtaccggacg taatccgaag actggcgata aagtagaact ggaaggaaaa tacgttcctc
     2341 actttaaacc aggtaaagaa ctgcgcgatc gcgccaatat ttacggttaa gtttttactc
     2401 aaacttgaac gcgagaaaag cacctgtcgg gtgctttttt catttctcta atctggaact
     2461 ggaagctgcc tcgcagagtt ttgagcagtt ttcaccctat cgttaaattc ttctgaacgt
     2521 gcctcgggga acgcaaaact cccaccagac aaccgttcaa caaagttgca cactttctat
     2581 aaacagggag ggtgcatgaa aataacgaca gtcggcgtat gcataatttg cggaattttt
     2641 ccgttgctga ttttgcccca attgcctggg acagtaaccc ttgcgtttct gactctcttc
     2701 gcctgtgtac tggcatttat ccctgttaaa accgtccgtt atatcgtgct gacgttgctg
     2761 tttttcgttt ggggcatatt agcagcaaag caaattttgt gggcaggaga aaccttaact
     2821 ggcgcgacgc aggatgcaat tgttgagatc attgctactg acggcatgac cactcattac
     2881 ggtcaaatta ctcatctaca aggtcgacgt atattccctg cgccaggcct cgtactgtat
     2941 ggcgaatatc ttccgcaagc ggtttgtgcc ggacaagtat ggtcaatgaa actcaaagtt
     3001 cgtgcagttc atggtcaact taatgatggc ggctttgata gccagcgtta tgctattgcc
     3061 cagcatcaac cgctcaccgg ccgttttctg caggcaagtg tcattgaacc gaattgtagc
     3121 ctgcgtgcac agtatctggc gtcaatacaa acaacgctgc aaccctatat gtggaacgcg
     3181 gttattcttg gattaggtat gggggaacgg ttatccgtcc ccaaagaaat caaaaatatc
     3241 atgcgtgata ctggaacggc gcatttaatg gcaatatcag gattgcacat cgcttttgcg
     3301 gcgttgctgg ctgcaggact cattcgcggt gggcaagttt ttctgcctgg gtgctgnnnn
     3361 nnnnnngctt gccatttggg gaatgcttaa gttaagtgga cgacaatgga gtggctggga
     3421 tgtatggata tgttgtctgg cggcaatttt gctgatggat cctgttgcca ttctctcgca
     3481 aagtttatgg ctatctgccg ctgcggtcgc ggcactgatt ttttggtatc agtggtttcc
     3541 ctgtccggag tggcagctgc cgccggtatt gcgcgcggtt gtttccctca tccatctgca
     3601 actgggaatc accctcctgc ttatgcccgt gcaaattgtc atatttcatg gcattagtct
     3661 gacctcgttt attgcaaatc tttttgcaat tcccctggtg acatttatca cggttccgtt
     3721 gattctcgcc gcgatggttg tgcatttaag cgggccgttt attctggaac aagggttatg
     3781 gtttcttgcc gaccggtctt tggctttact tttctggggg ttaaagagtt tgccggaagg
     3841 gtggatcaac attgctgaac gttggcaatg gctaacattt tccccatggc tcttactggt
     3901 ggtatggcga ttaaacgcct ggcgaacgtt gccagcaatg tgtgtggctg taggcttgct
     3961 gatgtgcttg ccgctgtggc aaaaacctcg acctgacgag tggcaagtgt acatgcttga
     4021 tgtcgggcaa gggctggcaa tggtcattgc gcgaaacggc aaagcgattc tctatgatac
     4081 gggactggcc tggcccgaag gggatagtgg gcaacaactg attatcccct ggctccactg
     4141 gcataatctt gaaccggaag gcgttatttt gagtcatgaa catctggatc accggggagg
     4201 gctggactca atattgcaca catggccgat gttatggatc cgaagtccgt taaactggga
     4261 acatcatcag ccctgtgtgc gtggcgaagc gtggcaatgg caaggattgc gtttcagcgc
     4321 gcactggcct ttacaaggta gcaacgataa aggaaataac cattcctgtg tggttaagat
     4381 tgatgacggg acgaatagca ttcttctaac cggtgatatt gaagcccccg ctgaacaaaa
     4441 gatgctaagc cgttactggc agcaaatgca ggcaacatta cttcaggtac ctcaccatgg
     4501 cagtaatacc tcatcatcgt tgccattaat tcagcgagtg aatggaaaag tggcactcgc
     4561 atcggcatcg cgctataacg catggcgact gccctcaagc aaagttaaac atcgctatca
     4621 acaacagggt tataagtggc ttgatactcc acatcagggt caaataacgg tcaatttttc
     4681 agcgcaaggc tggcggatta gcagcctcag agagcaaatt ttacctcgtt ggtatcatca
     4741 gtggtttggc gtgccagtgg ataacgggta gaatatgcgg ctatttcaac aaatgctggt
     4801 tttttgaatg cataacgaca aagatctctc tacgtggcag acgttccgcc gactgtggcc
     4861 aaccattgcg ccttttaaag cgggtctgat cgtggcgggc gtagcgttaa tcctcaacgc
     4921 agccagcgat accttcatgt tatcgctcct taagccactt cttgatgatg gctttggtaa
     4981 aacagatcgc tccgtgctga tgtggatgcc gctggtggtg atcgggctga tgattttacg
     5041 tggtatcacc agctatatct ccagctactg tatctcctgg gtatcaggaa aggtggtaat
     5101 gactatgcgt cgccgcctgt ttggtcacat gatgggaatg ccggtttcat tctttgacaa
     5161 acagtcaacg ggtacgctgt tgtcacgtat cacctacgat tccgaacagg tcgcttcttc
     5221 ttcttccggc gcactgatta ctgttgtgcg tgaaggtgcg tcgatcatcg gcctgttcat
     5281 catgatgttc tattacagtt ggcaactgtc gatcattttg attgtgctgg caccgattgt
     5341 ttcgattgcg attcgcgttg tatcgaagcg ttttcgcaac atcagtaaaa acatgcagaa
     5401 caccatgggg caggtgacca ccagcgccga acaaatgctg aaaggccata aagaagtatt
     5461 gattttcggt ggtcaggaag tggaaacgaa acgcttcgat aaagtcagta accgaatgcg
     5521 tcttcagggg atgaaaatgg tttcagcctc ttctatctct gatccgatca ttcagctgat
     5581 cgcctctttg gcgctggcgt ttgttctgta tgcagcaagc ttcccaagtg tcatggatag
     5641 cctgacttcc ggtacgatta ccgttgtttt ctcttcaatg attgcactta tgcgtccgct
     5701 gaaatcgctg accaacgtta acgcccagtt ccagcgtggt atggcggctt gtcagacgct
     5761 gtttaccatt ctggacagtg agcaggagaa agacgaaggt aagcgcgtga tcgagcgtgc
     5821 aactggcgac gtggaatttc gcaatgtcac ctttacttat ccaggacgtg acgtgcccgc
     5881 attgcgtaac atcaacctga aaattccggc agggaagacg gttgctctgg ttggacgctc
     5941 tggttcaggt aaatcaacca tcgccagcct aatcacacgt ttttacgata ttgatgaagg
     6001 cgaaatcctg atggatggtc acgatctgcg cgagtatacc ctggcgtcgt tacgtaacca
     6061 ggttgctctg gtgtcgcaga atgtccatct gtttaacgat acggttgcca acaacattgc
     6121 ttacgcaagg actgaacagt acagccgtga gcaaattgaa gaagcggcgc gtatggccta
     6181 cgccatggac ttcatcaata agatggataa cggtctcgat acagtcattg gtgaaaacgg
     6241 cgtgctgctc tctggcggtc agcgtcagcg tattgctatc gctcgagcct tgttgcgtga
     6301 tagcccgata ctgattctgg acgaagctac ctcggcgttg gataccgaat ccgaacgtgc
     6361 gattcaggcg gcactggatg agttgcagaa aaaccgtacc tctctggtga ttgcccaccg
     6421 cttgtctacc attgaaaagg cagacgaaat cgtggttgtc gaggatggtg tcattgtgga
     6481 acgcggtacg cataacgatt tgcttgagca ccgtggcgtt tacgcgcaac ttcacaaaat
     6541 gcagtttggc caatgatcga aaaaatctgg tctggtgaat cccctttatg gcggctattg
     6601 ctgccactct cctggttgta tggcctggtg agtggcgcga tccgtctttg ctataaacta
     6661 aaactgaagc gcgcctggcg tgcccccgta ccggttgtcg tggttggtaa tctcaccgca
     6721 ggcggcaacg gaaaaacccc ggtcgttgtc tggctggtgg aacagctgca acagcgcggt
     6781 attcgcgtgg gggtcgtatc gcggggatat ggtggtaagg ctgaatctta tccgctgtta
     6841 ttgtcggcag ataccaccac agcacaggcg ggtgatgagc ctgtgttgat ttatcaacgc
     6901 actggcgcgc ctgttgcggt ttctcccgtg cgttctgacg cggtaaaagc cattctggcg
     6961 caacaccttg atgtgcagat catcgtaacc gacgacggtt tacagcatta ccgtctggcg
     7021 cgtgatgtgg aaattgtcgt tattgatggt gtgcgtcgct ttggcaatgg ctggtggttg
     7081 ccggcggggc caatgcgtga gcgagcgggg cgcttaaagt cggttgatgc ggtaatcgtc
     7141 aacggtggtg tcccccgcag cggtgaaatc cccatgcatc tgctgccggg tcaggcggtg
     7201 aatttacgta ccggtacgcg ttgtgacgtt gctcagcttg aacatgtggt ggcgattgcg
     7261 gggattgggc atccgccgcg cttttttgcc acgctgaa
//
LOCUS       DAWDRC010000137         7283 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4895_length_7283_cov_2.770338, whole genome shotgun sequence.
ACCESSION   DAWDRC010000137 DAWDRC010000000
VERSION     DAWDRC010000137.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4895_length_7283_cov_2.770338"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1917 a         1722 c         1699 g         1945 t
ORIGIN      
        1 attgatttta acgatgaact gagaaatcgc cgcgaaaaac tggcggcact acgtcagcaa
       61 ggtgtggcgt ttcccaatga ttttcgccgc gaccatacct ctgaccagtt gcacgaagag
      121 tttgatgcga aggataacca ggaactggaa tccttaaaca ttgaagtctc ggttgctggc
      181 cgaatgatga cccgtcgtat tatggggaaa gcctcctttg taacgttgca ggatgtcggt
      241 ggccgtattc aactgtacgt tgcaagagat agcctgccag aaggtgttta taacgatcag
      301 tttaaaaaat gggatctggg tgacattatc ggtgcccgcg gtacgctgtt taagacgcaa
      361 acgggtgagc tttccattca ctgtactgag ctgcgcctgc tgactaaagc actacgtcct
      421 ttaccagata aattccatgg tctgcaggat caggaagtcc gttatcgtca acgttatctg
      481 gacctcatcg ctaacgataa atcccgtcaa acgtttgttg tccgttcaaa aattctggcc
      541 gctatccgtc agttcatggt cgcgcgcggc tttatggaag tagaaacccc gatgatgcag
      601 gtaattccag gtggggcatc tgctcgcccg tttattaccc atcataacgc tctggattta
      661 gatatgtacc tgcgtatcgc gccggagctg tatctgaaac gtctggttgt aggcggtttt
      721 gaacgggtat tcgaaatcaa ccgtaacttc cgtaatgaag gtatttctgt tcgccataat
      781 cctgagttca caatgatgga actctacatg gcatatgcgg attaccacga tttgattgaa
      841 ctgacggagt cactgttccg caccctggca caagaggtac tgggtaccac taaagtcact
      901 tatggcgagc atgtgtttga tttcggcaaa ccgtttgaaa aactcaccat gcgcgaagca
      961 atcaaaaaat atcgtccaga aaccgatatg gcggatctgg ataattttga tgctgctaaa
     1021 gcattagctg aatctatcgg tattacggta gagaaaagct gggggctggg acgtattgtt
     1081 acggagatct ttgatgaagt ggcagaagca catctgattc agccaacctt tattacggaa
     1141 tatccggcag aagtgtcccc gctggcgcgc cgtaatgatg ttaacccgga aatcaccgac
     1201 cgttttgaat tcttcatcgg tggtcgtgaa attggtaatg gttttagcga attaaacgac
     1261 gcagaagatc aggctgaacg tttccaggaa caggttaacg ctaaagctgc aggtgacgac
     1321 gaagccatgt tctatgacga agattacgtg actgcgctgg aatatggtct gccgccaacc
     1381 gctggtctgg gtattggtat cgaccgaatg attatgctgt ttacaaacag tcatactatt
     1441 cgcgacgtta ttctcttccc ggcgatgcgc ccacagaaat aaatttcact ttaatgaacg
     1501 aagcagtcag gcgactgctt cgttcgtcat tccgcaaatc cagttttgag gagagacgct
     1561 tcgtttcaag ataaccagcg ggtccactca actacagctc ctaaaagaga gaaaaaagta
     1621 atgccacagg aagactcatt ggccaggtta ttccgaccag aaatgcacta agtaaacgaa
     1681 tatgtgtttt ctcatgtgag ataaaccaga taatgacaaa ggatatgttc acaccaatca
     1741 cataaaaaat taatatttta gagaatagtg ccatttttta tgttcttctt atccttcctg
     1801 gctacttgta aaactgacgg aaatagtcat caactgtcat aatgctgagg tcaaaatcaa
     1861 tatccggtaa gtctaagcat ttgagcctga ttaaatcttc gatttcactt gcggctaacg
     1921 atgtcgttat tgcgaaggag ttttcattca gttgtcgtgg tatcccatat tcatcagcca
     1981 cgatagagga gaaattcata tcatacatca atgatcttaa ccttaagaac actgaggaat
     2041 aagatgatgg ataatcaaaa gaaacaacat aagtattgaa cttgttttta ccttccataa
     2101 tatgactcct atcaatattg aacgctctgg ggacgcaaaa cttattaacc ccgaatgatg
     2161 attctcatat aggagatatt cttttttttt tgctcagtaa aggggatgac ttgggacaaa
     2221 aaaacgctct ctctgaccat tctttgtgca tccccctacc cttcagatgc tctcctgcat
     2281 cacaccgcgg agagcatgaa aaatatcgtg actctgtact ctcccatcaa ctgcgaatat
     2341 catcatactc ccgtgtttta tcaaattcgt gtttcgcaaa tgggcatagt gggataattt
     2401 ttcgtttttc ccgacgcatt ttttccacga ctttcgcaac cagctgttta ccaatccctt
     2461 gtcctttcag gctttcatcg acatcggtat gttcgataat cgctaaattc tctccggtcg
     2521 gcacaaagac aatttcagcg atttgcttgc cctgttcgtc attaatgtaa aatttattgt
     2581 ggccttcgcg tatttccatt ttaccctcgc ttatttatga cggtatttca gcgcaccgct
     2641 cgggcacgta tcaatcactt taaccacagt ggtgacgtca acttcatccg gcatgatcca
     2701 cggcttacgt ttgagattaa ataacttgcc gttgccacgt acgcaattgc cagaatgctg
     2761 acatatcgca gtgttaaaat agacatcgat attttcgccg gtataacagc gataaccccc
     2821 gtccagtagc gcctgatcca tgaccttccc tctctcatca gtatggtttt cactataagc
     2881 atagccccgg aatcttcata cagcactggc agtttccgtt gccagagtct cttccccaat
     2941 atagccaatc attcttactc cctttgaatt acccgcctca tcagagataa tgcttaagaa
     3001 atttgtcaca caaggaagct gatgagacat tcattgccct accgtatgtt acgcaaacgt
     3061 ccgatgaaat tgagtaccac tgtgatctta atggtcagcg cggtactgtt ctcggtgcta
     3121 ttggtggtgc atctgattta cttctcgcaa atcagtgata tgacgcgaga cgggctagct
     3181 aacaaggcac tggcagtagc gcgtaccctc gccgactcgc cggaaatccg tcagggcttg
     3241 cagaaaaaac cgcaggagag cggtatccag gccatcgcgg aagccgtgcg caaacgcaac
     3301 gatctgctgt ttattgtcgt taccgatatg cacagtcttc gctactcgca tcctgaagcc
     3361 cagcgtattg gtcagccatt taaaggtgat gacatcctta aagcgctgaa tggcgaagaa
     3421 aatgtcgcta tcaatcgcgg ttttctggcg caggctttac gcgtatttac ccccatctac
     3481 gatgaaaatc ataaacaaat tggcgtggtg gcgatcggcc ttgagttaag ccgcgtgacc
     3541 caacagatca atgacagtcg ctggagcatt atctggtcgg tactatttgg catgctggtc
     3601 ggactgatcg gcacatgcat tctggttaac gtactgaaaa aaatcctttt cggcctggaa
     3661 ccctacgaaa tctccacact gtttgagcaa cgccaggcca tgttgcagtc catcaaagaa
     3721 ggcgtcgttg ccgtggacga tcgcggcgag gtcacgctga tcaacgatgc cgcacaagaa
     3781 ttgctgaatt accgtaagtc gcaggacgat gagaaactgt cgacgctaag ccactcgtgg
     3841 tcacaggtgg tagatgtctc ggaagtgtta cgcgacggaa ccccacgccg cgacgaagag
     3901 attacgatta aggaccgact attactgatc aacaccgttc cggtgcgcag taatggcgtt
     3961 atcatcggtg ccatttcaac cttcagggac aaaactgaag tacgtaaact gatgcagcga
     4021 ctcgacggtc tggtcaacta tgctgacgca cttcgtgaac gatcccacga atttatgaat
     4081 aaattgcatg tgattctcgg attattgcat ctgaagagtt ataagcagtt ggaagattac
     4141 attctcaaaa cagccaataa ctatcaggaa gagattggct ctctgctggg taagatcaaa
     4201 tctccggtta tcgctggttt tttaatcagc aagattaacc gcgcgaccga tttaggccat
     4261 acactgattt taaacagtga aagccagctg ccagacagcg gtagtgagga tcaggtcgcg
     4321 acgctgatta ccacgttggg aaatctgata gaaaacgcgc tggaggcatt agggccggaa
     4381 cccggtggcg aaattagcgt aacattgcac taccgtcacg gctggctgca ctgcgaagtt
     4441 aacgatgatg gaccggggat cgcacccgac aaaatcgatc acatttttga caaaggtgtc
     4501 tcgacaaaag gaagcgagcg aggcgtcggt ttagcacttg tcaaacaaca ggtagaaaat
     4561 ctcggcggca gcatcgccgt ggaatcggaa cccgggattt tcacacaatt ttttgtccag
     4621 ataccctggg acggggagag gtcgaacaga tgatcaatgt attaattatc gatgatgacg
     4681 caatggtcgc ggagctgaat cgccgatacg tggcacaaat cccgggcttt caatgctgtg
     4741 gaacggcttc gacgctggag aaagccaaag agattatctt caatagcgat tcgcctatcg
     4801 acctgatatt gctagatatc tatatgcaaa aagagaacgg gctcgattta ctgcctgtcc
     4861 tgcataacgc gcgttgcaaa agtgatgtga ttgtcatctc ctccgctgcc gatgcagtaa
     4921 ccattaaaga ttcgctgcat tacggtgttg tggattacct gatcaaaccc ttccaggctt
     4981 cccgctttga agaggcgctc accggctggc ggcaaaagaa aatggcgctg gaaaaacatc
     5041 agtattatga ccaggccgag cttgatcagc taatccacgg cagcagctcc aacgagcagg
     5101 atcctcgtcg cttgccaaaa ggcttaacgc cgcagacgtt acgcacgctg tgccagtgga
     5161 ttgacgcgca tcaggactat gagttttcaa ccgacgagct ggctaacgag gttaacatct
     5221 cgcgtgtttc ctgccgtaaa taccttatct ggctggtcaa ctgccacata ttgttcacca
     5281 gtatccatta tggcgtcacg gggcgtccgg tttaccgtta ccgtattcag gcagaacact
     5341 actcactact gaaacaatat tgccaataac ggatcgctgg ttatctgtaa gtaataatta
     5401 ttaccgtaat gcaaaatacc cctggcacaa gttacatcgt tcatcagtaa aacttgtgcc
     5461 agatcaaata taattatccc tccatcatcg ctaaaaatta atatctcttc agatgaacgg
     5521 tgtttttaat ttcaaaacgc taacaaaagt taattaacta ttatgtcacc cacattatgt
     5581 gtatttttac ccacaaatgg gtagatcaga ttaatctata aacctaatga tacctgccct
     5641 gagaacaaaa aacagaccga taaatatcaa taagataaca gcaaacaaaa cattaacatc
     5701 tgcgctgtac aaactataaa cccatcgcca gagagtcttt ctctctgaaa aagccgctta
     5761 tcacagtgca taaatttgcc gctgctttaa tcagccaata ttcactgtga ggtatttgct
     5821 caagccggta acgaccaaac ggatatttag tcaggctctg aaaacagttc atacaaaaca
     5881 gaacgtgact gtgatctatt cagcaaaaat ttaaatagga ttatcgcgag ggttcacaca
     5941 tgttatttac tatccaactt atcataatac tgatatgtct gttttatggt gccagaaagg
     6001 ggggtatcgc gctgggttta ttaggcggta tcggtctggt cattctggtc ttcgtcttcc
     6061 accttcagcc aggtaaacca ccggttgatg tcatgctggt tatcattgcg gtggtggcgg
     6121 catcggcgac cttgcaagct tcgggcggtc ttgatgtcat gctgcaaatt gccgagaagc
     6181 tgctgcgtcg caacccgaaa tatgtctcaa ttgtcgcgcc gtttgtgacc tgtacgctga
     6241 ccattctttg cggtacgggt catgtggttt acaccattct gccgatcatc tacgacgttg
     6301 ctattaagaa caacatccgt ccggaacgtc cgatggcggc aagttctatc ggtgcacaga
     6361 tggggattat cgccagtccg gtgtcggttg cggtcgtatc tctggttgcg atgctgggta
     6421 atgtcacctt tgatggtcgc catcttgagt tcctcgacct gctggcaatc accattccat
     6481 cgacgttaat cggtatcctg gcgatcggta tcttcagctg gttccgcggt aaagatctgg
     6541 ataaagacga agagttccaa aaattcatct ccgtaccgga aaaccgtgag tatgtttacg
     6601 gtgataccgc gacgctgctg gataaaaaac tgccgaaaag caactggctg gcaatgtgga
     6661 tcttcctcgg ggcaatcgct gtagtcgccc ttcttggtgc tgattcggac ctgcgtccat
     6721 ccttcggcgg caaaccgctg tcgatggtac tggttattca gatgtttatg ctgctgaccg
     6781 gggcgctgat tattatcctg accaaaacca atcccgcgtc tatctcaaaa aacgaagttt
     6841 tccgttccgg tatgatcgcc atcgtggcgg tgtacggtat cgcatggatg gctgaaacca
     6901 tgttcggtgc gcatatgtct gaaattcagg gcgtactggg tgaaatggtg aaagagtatc
     6961 cgtgggccta tgccattgtt ctgctgctgg tttccaagtt tgtaaactct caggctgcgg
     7021 cgctggcggc gattgttccg gtcgcgctgg cgatcggcgt tgatccggca tacatcgtgg
     7081 cttcagcacc ggcttgctac ggttattaca tcctgccgac ttatccgagc gatctggcag
     7141 cgattcagtt tgaccgttcc ggcaccaccc acatcggtcg cttcgtcatc aaccacagct
     7201 ttattctgcc ggggttgatt ggtgtgagcg tatcgtgcgt cttcggctgg atcttcgccg
     7261 cgatgtacgg gttcttataa atg
//
LOCUS       DAWDRC010000138         7181 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4973_length_7181_cov_2.613528, whole genome shotgun sequence.
ACCESSION   DAWDRC010000138 DAWDRC010000000
VERSION     DAWDRC010000138.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4973_length_7181_cov_2.613528"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1860 a         1843 c         1742 g         1736 t
ORIGIN      
        1 ttcagatgtc agattatgtc caacatttgg ggtgcagttc attgcgcgtc ttatcaggcc
       61 tacgagtcgg atacacatcc ggcatcacac agactctgaa accgtgcggg ggcaaccccg
      121 cacacatcaa taatcccgcc cttcccctgt gctacacttc gcgccattcc ttactgctta
      181 gagtttgcta tgagacgaga acttgccatc gaattttccc gcgtcaccga atcagcagcg
      241 ctggctggct acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta
      301 aacgccatgc gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa
      361 ggtgaaatcg acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc
      421 gacgcggtag atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag
      481 gctaacgcgc tggcggtgct ggcagtaggc gataaaggct gtttcctcaa cgcgccagat
      541 atgtatatgg agaagctgat cgtcggaccg ggagccaaag gcaccattga tctgaacctg
      601 ccgctggcgg ataacctgcg caatgttgcg gcggcgctcg gcaaaccgtt gagcgaactg
      661 acggtaacga ttctggcaaa accgcgccac gatgccgtta tcgcagaaat gcaacaactc
      721 ggcgtacgcg tatttgctat tccggatggc gacgttgcgg cctcaattct cacctgtatg
      781 ccggacagtg aagtcgatgt actgtacggc attggcggcg cgccggaagg cgtggtttct
      841 gcggcggtga tccgcgcatt agatggtgac atgaacggtc gtctgctggc gcgtcatgac
      901 gtcaaaggcg acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa
      961 gcgatgggca tcgaagccgg taaagtattg cgtttgggcg atatggcgcg cagcgacaac
     1021 gtcatctttt ctgccaccgg catcaccaaa ggtgatctgc tggaaggtat tagccgcaaa
     1081 ggcaatatcg ccaccaccga aacgctgctg atccgtggta aatcacgcac cattcgtcgc
     1141 attcagtcca tccactatct ggatcgtaaa gacccggaaa tgcaggtgca catcctctga
     1201 ttgatttgat cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac
     1261 gaagataagg caagtcaatc aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa
     1321 gtcactaaag tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgctcccgtg
     1381 cttccgttta ccgccgggca attcaccaaa cttggccttg aaattgaagg cgaacgcgtt
     1441 cagcgcgcct actcctatgt taactcgccc gataatccgg atctggagtt ttacctggtc
     1501 accgtccccg atggcaaatt aagtccacga ctggcggcac tgaaaccgga cgatgaagtg
     1561 caggtggtta gcgaagccgc tggcttcttt gtgctggatg aagtccctga ctgcgaaaca
     1621 ctgtggatgc tggcgactgg tacggcgatt ggcccttatt tatcgattct gcaattgggc
     1681 aaagatttag aacgcttcaa aaacatggtg ctggttcacg ccgcacgata tgccgccgac
     1741 ttaagctatc tacctctgat gcaggaactg gaaaaacgct acgaggggaa actgcgcatt
     1801 cagacggtgg tcagtcggga aacggctgcg ggttcgctca ctgggcgatt accggcgtta
     1861 attgaaagtg gtgaactgga aagcgcggtt ggcctgccga tgaataaaga aaccagccat
     1921 gtgatgctgt gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaggagacc
     1981 cggcagatga cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg
     2041 taagcggtta cttgtcgata aacggcacga tcaagagatc cgcactcaac ttattgatca
     2101 tcccgcgata tgccggcatc aaacggttga taaatgagtg gtgatgacca cagacaagga
     2161 ggtcgcactg ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa
     2221 tacgcagttt tgtcttcggc cattgaatat ttttcgtcag tttatacagc ttgttatccg
     2281 acttattctt caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca
     2341 actcgcttaa gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga
     2401 gctccagggc tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa
     2461 tgtgtttata agccatattt aactccttct aaagcaactc catcaagcta acgaacgcag
     2521 tgataactca aaaataatca tctcggcctg gcatgagaag gtgaaggccg catcaagctc
     2581 aacttgccct tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga
     2641 tgcagcggcg gcaagctctt tcagtttggt cataaaggac tctgcttctt cgcgggtggc
     2701 aaaaaagcgc gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat
     2761 cgcacatcct ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt
     2821 cgcctctctg tcgattacga cttaagaata atgcgccgtt aaaggaagaa caaacaagta
     2881 atgatcttat ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt
     2941 ttccgggcat tgtcacttgc atcgtggaaa taacaatccc ggaaaaaaat agcgataaga
     3001 gatatattaa aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgggtat
     3061 ctttaccata tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca
     3121 tcaccagaat caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa
     3181 tcgcccagtt acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg
     3241 aacgcccgcg atcatgcaag cgcttaacgg ttactgccgc tgttggccaa agcgagcaca
     3301 caaggcaaaa cgccgcggtc tgaatatcga gtaaattttt acccgccagt gagaaaagca
     3361 ccagcatgcc tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc
     3421 ctttaaatga gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc
     3481 caggattcta cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac
     3541 aaaaggtact gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa
     3601 ccgttacaac aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc
     3661 tggctggagc ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc
     3721 agaatcccag cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca
     3781 atctcactcg tgcagcaagt aaaattaacg agaacttgta tgcttctact gcgctggagc
     3841 gcggtacctt aaaaatcaaa agcattcaaa taacctggct acccatccag gggccagagc
     3901 aaaaagccgc gaaagcgaaa gctcaggcat acatggcagc ggtgatccgc acactcaccc
     3961 cattaatgac caaaactcaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa
     4021 acaaacgtta ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg
     4081 aaaaggggct gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag
     4141 gtttgaataa atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca
     4201 gacctgctgc cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa
     4261 aatgcgacat cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca
     4321 cgagctggta tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat
     4381 cgcaccaccg gaaatgtaca tcgatatggc gaagcgtgaa gctgaaggca gccacatcat
     4441 gctgggtgcg caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacctctgc
     4501 tgctatgctg aaagacatcg gcgcacagta catcatcatc ggccactctg aacgtcgtac
     4561 ttaccacaaa gaatctgacg aactgatcgc gaaaaaattc gcggtgctga aagagcaggg
     4621 actgactccg gttctgtgca tcggtgaaac cgaagcggaa aacgaagcgg gcaaaactga
     4681 agaagtttgt gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga
     4741 aggtgcggtt atcgcttacg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc
     4801 ggctcaggca caggctgttc acaaattcat ccgtgaccac attgctaaag ttgacgctaa
     4861 catcgctgaa caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga
     4921 actgtttgct cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga
     4981 cgctttcgcc gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt
     5041 gccggatttc atatccggca cttactttcc taaactcctc gccttaacgc aaaatctcac
     5101 actgatgatc ctggatttct tcggctgaag cacggttcag cgtcagtaga tttcgttgtg
     5161 tcgccagcaa tacaaatgag ttatcactct gccgtaccat cgccagcccg tagcttccca
     5221 tatgttcccg cgcctcaggc acttcttccg ccagcatcat aaatgggctg cgttgtacca
     5281 gttcgctttc cgttacccga cgcgccaggt actcatgccc acgcaaacca cctggcagtg
     5341 gcaaccagcg gctactgatg ttcgccagat tgttatccag ctgttcgcgc acatcaggac
     5401 gaatacaaga aatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg
     5461 ccagagatac cgcgcgatcg ggaacaggct ggccgtattt ttggctcata aaatcacgcg
     5521 cctgccaggc taaccagaaa aaattaggcg ttgacggatc ggtcaacaat gggctttcag
     5581 taccgttaat acggtacgtt ggcatcaaca gatattgcag tgggccatga cgatctttta
     5641 aaaccacgta tccggcgttg ggtttgactt ccgcacatgg cgacgggttt tggttttcct
     5701 gctggttggg caaacattgc tcaagaacaa tcttacgtaa cgtgtctgac tcttcaccgg
     5761 ttaatttcca gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa
     5821 gaagacccgc ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca
     5881 aaatcgtgac aaataaaaaa cccagtggcg agaaaccacc gggcttgata attatcctgg
     5941 ggaaatcagc gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc
     6001 gctttcgtcc agccgccgaa ctcttcatca atggtgaata acttcaattt tggaaacgca
     6061 ttttcgtact ttttcgctac ctcagcgtcg cgcggacggt agtagttttt cgctgcaatt
     6121 tcctgacctt ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgtct
     6181 tttttctcga ccactttatc gaccaccgac acggttggct ctgcgaggat agactcactc
     6241 ggcgtgacga tttcgaattt atctttcccc agttcattcg ctgccaccag tgttacagtt
     6301 tcccaggcaa tcagtacatc gccaattccg cgctcgacaa aagtgttagt ggatccgcgc
     6361 gcgccagaat ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt
     6421 gcctgatcgt tgttgttgtg atgcagcgcg tagccccagg ctgccaggta attccagcgc
     6481 gcgccaccag agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg
     6541 ttccagtcat ggatctgctt cggattaccc ttacgtacca ggaaaacaat ggtggaagtg
     6601 tacggtgcgg agttatccgg tagacgtttg atccactctt tatcaatccg cccgcgttcc
     6661 gcaattgcgt ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg
     6721 attaccgacg tcgcttgttt gcctgaacca ccgtgtgact gacgaatcac cacgttatcg
     6781 ccagtttgct gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc
     6841 gttggatcat atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc
     6901 agcaaaaatg ttaatcctac gccccacttt ttcatcgccc gactctctta tgttgtgttg
     6961 tgatgagcaa agcgtgccag aaggttgacc aaacattaaa gaataaaaaa agattgacta
     7021 taacttgcga gtatatgttg ggggattaaa aaggcggaag aaatccgcct catattgctg
     7081 acaaagtgcg ctttgtccat gccggatgcg gcacaaacgc cttatccgac ctacaaaagt
     7141 ttgcaaattc aatagattac agaattcatg taggcctgat a
//
LOCUS       DAWDRC010000139         7162 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4988_length_7162_cov_2.526664, whole genome shotgun sequence.
ACCESSION   DAWDRC010000139 DAWDRC010000000
VERSION     DAWDRC010000139.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4988_length_7162_cov_2.526664"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2776..2785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4989..4998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6928..6937
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1489 a         1747 c         2067 g         1829 t
ORIGIN      
        1 tgccggtata tggcaacggg cagcaaatcc gtgactggct gtatgttgaa gatcacgccc
       61 gcgcgctgta ttgcgtggcg accaccggga aagtcggtga aacctataat attggtggtc
      121 acaacgagcg taagaatctc gatgttgtgg aaaccatttg cgagctgctg gaagaactgg
      181 ctccgaataa gccgcacggc gtggcgcact atcgtgacct gatcaccttt gtcgctgacc
      241 gtccggggca tgatctgcgt tatgccattg atgcttcgaa aattgcccgt gaacttggct
      301 ggctgccgca ggaaaccttt gaaagtggaa tgcgtaaaac ggtgcagtgg tatctgggta
      361 atgaaatctg gtggaagcag gtgcaggacg gcagctatca gggcgagcgt ttaggtctga
      421 aaggctaatt ttcagcggag gcaaacatga aaggtattat cctggcgggc ggttccggca
      481 cccgattgca tccgattacg cgcggcgtat cgaagcaact gttgccgatt tacgataagc
      541 caatgattta ctatccgctg tcggtgctga tgttggccgg tatccgcgaa attctcatca
      601 tcactacgcc ggaagataaa ggttatttcc agcgcctgct gggcgatggt agtgagttcg
      661 gtatccagct ggaatacgcc gaacagccca gtccggacgg tctggcgcag gcctttatca
      721 tcggtgaaac cttcctcaac ggtgaacctt cttgtctggt gctgggcgat aacatcttct
      781 tcggacaggg tttcagtccg aagctgcgtc atgttgcggc gcgcacggaa ggggcgacgg
      841 tatttggcta tcaggtgatg gacccggaac gctttggcgt ggtggagttt gacgacaatt
      901 tccgcgctat ctcgctggaa gaaaaaccaa aacagccgaa atcaaactgg gcggtgaccg
      961 ggctttattt ctacgacagt aaagtcgtgg agtacgcaaa gcaggtgaag ccgtcggagc
     1021 gtggtgaact ggagattacc tccatcaacc agatgtacct cgaggcgggc aacctgaccg
     1081 ttgaactgct cgggcgcgga tttgcctggc tggataccgg cactcacgac agcctgattg
     1141 aggccagcac ttttgtgcag acagtagaaa aacgccaggg ctttaagatt gcctgcctgg
     1201 aagagattgc ctggcgtaac ggctggctcg atgacgaggg tgtgaagcgt gctgccagtt
     1261 cattagcgaa aactggctac ggccaatatc tgctggagtt acttcgtgcc cgtccgcgcc
     1321 agtattgagc cactaacctg ggaaaacgcc ttctttggtg ttaacagcgc catcgtgcgc
     1381 attacgtctg aagcaccgct cctgacgcca gacgcgttag cgccgtggtc acgggtgcag
     1441 gccaaaattg cggcatcaaa tacgggtgaa ctggatgctc tgcaacagct gggattctcc
     1501 ctggtagaag gtgaagttga tttggcacta cccgtgaaca atgtcagtga tagcggtgca
     1561 gtagtggcac aagagaccga tattcccgca ttacgtcagt tagccagcgc cgcatttgcg
     1621 caaagccgtt ttcgtgcgcc gtggtatgcg cctgacgcca gcggtcgctt ttatgcacag
     1681 tggattgaaa atgccgtgcg cggcaccttt gatcatcaat gtctgatttt acgtgcggcg
     1741 tccggcgata ttcgcggtta tgtctcttta cgggaactca atgcgacaga tgcgcgaatt
     1801 ggcctgctgg ctggacgcgg tgcaggtgct gagctaatgc aaacggcgct aaactgggcg
     1861 tatgctcgcg gtaaaacaac tttgcgggtg gcgacccaga tgggcaacac cgccgcgctt
     1921 aaacgataca tacaaagtgg tgcgaatgta gaaagcaccg cgtactggtt atacaggtga
     1981 tcacatgatt ccatttaacg caccgccggt ggtgggaacc gaactcgact atatgcagtc
     2041 ggcaatgggt agcggcaaac tgtgcggcga tggtggtttt acccgtcgct gccagcagtg
     2101 gctggagcaa cgttttggca gcgccaaagt gttactgacg ccatcctgta ccgcttcgct
     2161 ggagatggcg gcgctgctgc tcgatatcca gcctggcgat gaagtgatca tgccgagcta
     2221 tacctttgtc tccaccgcca atgcctttgt gctgcgtggc gcgaaaatcg tctttgtgga
     2281 tgttcgcccg gacaccatga acatcaacga aacgctgatt gaagcggcga tcaccgacaa
     2341 aacgcgcgtt atcgtgccgg tccattacgc gggtgtggcc tgcgaaatgg acaccattat
     2401 ggcgttggcg aagaagcata atctgtttgt ggtggaagat gccgctcagg gcgtgatgtc
     2461 cacttacaaa gggcgtgcgc tgggaaccat tggtcatatt ggctgcttta gcttccatga
     2521 aaccaaaaac tacacggcgg gtggtgaagg cggcgcgacg ctgattaacg ataaagcgtt
     2581 gatcgaacga gccgagatca tccgtgaaaa aggcacaaac cgcagccagt tcttccgtgg
     2641 tcaggtcgat aaatatacct ggcgcgatat tggctccagc tatttgatgt ccgatctgca
     2701 agctgcgtac ctgtgggcgc aactggaagc tgcggatcgt atcaaccagc aacgtctggc
     2761 gctgtggcaa aactannnnn nnnnnaaaac tactacgatg cgctggcgcc tctggcaaaa
     2821 aatgggcgta tcgagctgcc gtcgattccc gatggctgcg tgcagaacgc gcatatgttc
     2881 tacattaaac tgcgggatat tgctgaccgg agcgcattga ttaactttct gaaagaagcg
     2941 gaaatcatgg cggtgttcca ttacattccg ctgcacggtt gccctgcggg ggaacgcttt
     3001 ggtgagttcc acggcgaaga tcgctacacc accaaagaga gcaagcgcct gctgcgcctg
     3061 ccgttgttct acaacctgtc gcccgtcaat cagcgtacgg taattgcgac cttgttgaac
     3121 tacttctcct gatatgtcgt tggcaaaagc gtccttgtgg acggcggcca gtacactggt
     3181 caagattggt gccgggttac tggtcggtaa gttgctggca gtgtcatttg gtccggcggg
     3241 gcttgggctg gcggcaaatt tccgccagtt gattaccgtg ctcggcgtgc ttgccggggc
     3301 aggcatcttt aacggcgtaa ccaaatacgt tgcccagtat catgataatc cccaacagct
     3361 gcgccgcgta gtcggcactt catcagcgat ggtacttggc ttctctacgc taatggcgct
     3421 ggtttttgtg ctggcagctg cgccaatcag ccaggggttg tttggtaata ccgactatca
     3481 ggggctggtg cgtttagtgg cgctggtgca aatggggatc gcctggggca acctgttact
     3541 ggcgttgatg aaaggctttc gcgatgccgc aggtaatgcg ttatctctga ttgtcggtag
     3601 cttgattggc gttctcgcgt actacgtcag ttatcgtttg ggcggttatg aaggggcgtt
     3661 gctgggtctg gcgctgattc ccgcgctggt ggtaattcct gccgccatca tgttgatcaa
     3721 acgtggtgtc atcccgttaa gctatctgaa acccagctgg gataacggtc tggcagggca
     3781 gttgagcaaa tttacgctca tggcgttgat tacgtcggtg accttgcctg ttgcttacat
     3841 catgatgcgt aaactgctgg cggcgcagta tagctgggat gaagtgggga tctggcaagg
     3901 ggtgagcagt atttccgatg cctacctgca atttattacg gcatcgttca gcgtatattt
     3961 gctgcccacg ttgtcgcggt taacggaaaa gcgcgatatc acccgggaag tggttaaatc
     4021 gctgaaattc gtcttaccgg cagtggcggc ggcgagtttt accgtctggt tgctgcgtga
     4081 ttttgctatc tggctgctgt tgtcgaataa atttaccgct atgcgcgatc tctttgcctg
     4141 gcagttggtg ggtgatgtgt taaaagtggg cgcttatgtc tttggttatc tggtgatcgc
     4201 caaagcgtca ctgcggtttt atattctggc ggaaatcagc cagttcactt tattgatggt
     4261 atttgcccac tggctaatcc ctgcgcatgg cgcactgggc gcagcacagg cctatatggc
     4321 aacctatatc gtctattttt ctctttgttg tggcgtgttt ttactctggc gtaggcgggc
     4381 atgactgtac tgattcacgt actgggatcg gatatccctc accataaccg aaccgttctg
     4441 cggtttttca acgacgcgct ggccgcgacg agcgggcacg cgcgcgagtt tatggttgct
     4501 ggcaaggacg acggcttaag tgatagctgt ctggcgcttt ctgtgcaatt tttccctggg
     4561 aaaaaatcgc tggcggaagc ggtcatcgcg aaagcaaaag ctaaccgtca gcagcgattt
     4621 tttttccacg gtcagttcaa tcccaaactg tggctggctc tgctgagtgg tggcattaag
     4681 cccagccagt ttttctggca tatctgggga gcagacctgt atgagctttc cagtggcttg
     4741 agatataagc ttttttaccc gctacgtcgc ctggcgcaaa agagagtcgg ctgtgtattt
     4801 gccacccgcg gtgatttgag cttttttgcc aaaacgcatc caaaggtgcg gggagaactg
     4861 ctgtacttcc cgacgcggat ggacccttct cttaatacga tggcgaacga tcggcaacgt
     4921 gaaggaaaaa tgaccattct ggtggggaac tccggtgacc gcagcaatga gcatattgct
     4981 gccttgcgnn nnnnnnnntt ggtacgcttt gcttactgat tcaggcgggc attccttgcg
     5041 tgcttaaccg ggaaaatccg ttctggcagg atatgacgga acagcatttg ccagtactgt
     5101 ttactaccga tgatctcaac gaggatattg tgcgtgaagc gcagcgccag ttggcgtcgg
     5161 tggataaaaa caccattgcc ttctttagcc ctaactatct acaaggctgg cagcgggcgt
     5221 tggcggttgc cgccggggag gtcgcatgag tctgctgcaa ttcagtggcc tgtttgttgt
     5281 ctggctgctc tgcacgctgt ttattgccac gctgacctgg tttgagtttc gccgtgtgcg
     5341 ctttaacttc aatgtcttct tttcattgct gtttttgctc acctttttct tcggctttcc
     5401 gctgaccagc gtgctggtat ttcgctttga tgtaggtgtc gcgccgccag aaatcttgtt
     5461 gcaggcgttg ctttctgcag gctgcttcta cgcggtttac tatgtcacct acaaaacccg
     5521 tctacgcaaa cgcgttgctg atgcaccgcg ccgtccgctg tttaccatga accgcgtgga
     5581 gaccaatctc acgtgggtga tcctgatggg catcgctctg gtgagcgtcg gcatcttctt
     5641 catgcacaac ggctttttgc tgttccggct taattcttac agtcagatct tttccagcga
     5701 agtctccggc gtggcattaa aacgcttctt ttactttttc atcccggcga tgctggtggt
     5761 ttattttctg cgccaggata gcaaagcgtg gctgtttttc ctcgtcagca cggttgcctt
     5821 tggcttgttg acttacatga ttgtcggcgg cactcgcgcc aatatcatca tcgcattcgc
     5881 tatcttcctg tttattggca ttattcgcgg ctggatttcg ttgtggatgc tggcggcggc
     5941 gggcgtgctg gggattgtgg gcatgttctg gctggcgcta aaacgctatg gaatgaatgt
     6001 gagcggcgat gaagcgttct atacgtttct ctatctcact cgcgacacct tctcgccgtg
     6061 ggagaatctg gcgttgctgt tgcagaacta cgacaacatc gacttccagg gcctggctcc
     6121 aattgtccgc gatttctatg tctttatccc ttcctggctg tggccgggtc gcccgagtat
     6181 ggtgctgaac tccgccaact actttacctg ggaagtactg aataaccact ccggactggc
     6241 gatctcgcct acgcttatag gctcactggt tgttatgggc ggcgcgttgt tcatcccgct
     6301 cggggcgatt gtggttggac tgatcatcaa atggttcgac tggctgtatg agctgggcaa
     6361 ccgcgagact aatcgctata aggctgcgat attgcacagt ttctgcttcg gggcgatctt
     6421 caatatgatc gtgctggcgc gtgaagggct ggattcgttt gtctcacgcg tggtcttttt
     6481 tatcgtggtc ttcggcgcat gtctgatgat cgcaaaactg ttgtactggc tttttgaaag
     6541 cgccggactc attcataaac gaacaaaatc atcgctccgg acgcaggttg aaggataaca
     6601 atgaataaca acaccacggc accgacctat acgctgcgtg gcttacagtt gattggttgg
     6661 cgtgatatgc agcatgccct cgattatctg ttcgctgacg ggcagcttaa gcagggaacg
     6721 ctggttgcca ttaatgccga aaaaatgctg accattgaag ataacgccga ggtcagggag
     6781 ttaattaacg ctgccgaatt taaatatgcg gatggcatca gcgttgtacg ttcagtacgt
     6841 aaaaagtacc cgcaggcgca ggtttcccgc gttgctgggg ccgatctctg ggaagagctg
     6901 atggcgcgcg caggcaaaga agggacannn nnnnnnnttg tgggcggtaa acctgaagtg
     6961 ctggcgcaaa ctgaagctaa actgcgcaac cagtggaatg tgaatatcgt tggcagtcag
     7021 gatggttatt ttaaacccga gcagcgtcag gcgctgtttg aacgcattca tgccagcgga
     7081 gcgcaaatcg tcaccgttgc gatgggatcg ccaaagcagg agatcttcat gcgcgactgc
     7141 cgtctggtgc atccagatgc gc
//
LOCUS       DAWDRC010000140         7158 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_4991_length_7158_cov_2.138955, whole genome shotgun sequence.
ACCESSION   DAWDRC010000140 DAWDRC010000000
VERSION     DAWDRC010000140.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7158)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4991_length_7158_cov_2.138955"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3840..3849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4897..4906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1635 a         1870 c         2022 g         1611 t
ORIGIN      
        1 cagttggtag agcacgacct tgccaaggtc ggggtcgcga gttcgagtct cgtttcccgc
       61 tccaaattct tctctcaata aaatatccac agcgacgcga tgcgttattg ctggtttttg
      121 atgtctctga cagactcttg taaacagagt tatccacagc ctcaggccgt aatcttaatt
      181 tcaaagaaac ttcgcacggt gaatagtatt tttttaacct attgatagat aagttaaaaa
      241 ttaagatttc gttttgtcgt atcgatcact tgacgatttt ttccggcttg aattgcaatg
      301 cgtttttatt tttattcaca agctgtggat gaatcaggcg tcacgcggta accctttttc
      361 aatcacccga accagacgct gttttttcgg taattgcact tcgaccacgc acgcatttcg
      421 cctctcgatt tgctgcgcaa tcgcccacgc tatgtgctca tcaagaagtg ggtgctcacc
      481 tttacgactt tccagcgctg tcaaaatcgt ttcatcccag ggggcattgc ctaatgctac
      541 ggcgatatta cgcagccaac gcaggtgacc tatacggcga atcgccgaac cttccgtgac
      601 ttttaaaaac ttctcttcac tccaggcgaa taactcaatg agttccggtg cgtgtagcgg
      661 cttacgcggg ctgaaatcgt cttctgtagt gagttgcgaa tagcgattcc acgggcagat
      721 aagctggcag tcatcgcaac cgtaaatacg gtttcccatt aacggtcgca actcttccgg
      781 gatcgcccct tccagttcga tggtgagata agagatacag cggcgagcat cgacggtata
      841 tggctcgacg atggctccgg tcgggcaaat cgtcatacag gccacgcatt taccgcatcc
      901 ttcctcgact ggttgatcca caggcagcgg aatatcgacc agcaattcgc ctaaaaagaa
      961 gaacgaaccg gcctcgcgat tgaggataag tgagtgctta cctgtccagc caagacctgc
     1021 tttttcagct aacgggcgct cgagaatagg cgcagaatcg acaaacggtc taaaattcag
     1081 cgaaacacaa tgttgctgaa tcatctcgcc cagctttttg agtcggttgc gcagaagctt
     1141 gtgatagtca cggcccagcg cataacggct aacatagccg agtttgggat ttttcagtgt
     1201 gctggcaaat gcggcgttag caggaaggta attcatccgc acgctgatca cgcgcagcgt
     1261 accgggcaat aattcgtgag gacgagcacg cagcataccg tgacgcgcca tccagtccat
     1321 ttcgccgtgg tattgtttgt ccagccatgc ttgcagtttg ggctcggacg cgctgagatc
     1381 ggtatcggta atacctacct gctgaaagcc cagttccagc ccccactgtt taatattttg
     1441 cgctaattga ttgagatcga ggggctctga catgacggac catacaatga agaaaaaccc
     1501 cgtaagtata ccacacaccg tctggtatgc cgacgatatc cgccgcggag aacgcgaggc
     1561 ggcagatgcg ctggggctca cactctatga gctgatgctt cgcgctggcg aagccgcatt
     1621 ccaggtgtgt cgttcggctt atcctgacgc ccgccactgg ctggtgctgt gcggtcatgg
     1681 taataacggc ggcgatggct acgtggtcgc gcggctggcc aaagcgatcg gtattgatgt
     1741 cacgctgctg gcccaggaaa gtgacaaacc gttgccggaa gaggccgcgc tggcacgcga
     1801 agcatggtta aacgcgggag gcgagatcca tgcttcgaat attgtctggc ccgaatcggt
     1861 agatctgatt gttgatgcgc tgctcggcac cggcttgcag caagcgcccc gcgaatccat
     1921 tagccagtta atcgaccacg ctaatactca ccctgcgccg attgtggcgg ttgatatccc
     1981 ttccggcctt ctggctgaaa ccggcgctac gccaggcgcg gtaatcaacg ccgatcacac
     2041 catcactttt attgcgctga aaccaggctt gctcactgga aaagcgcggg atgttaccgg
     2101 acaactgcat tttgactcac tggggctgga tagctggctg gcaggtcagg agacgaaaat
     2161 tcagcggttt tcggcagaac aactttctca gtggctgata ccgcgtcgcc cgacttcgca
     2221 taaaggcgat cacgggcggc tggtgattat tggtggcgat cacggcacgg cgggggctat
     2281 tcgtatgacg ggggaagcgg cgctgcgtgc tggtgctggt ttagtccgag tactgacccg
     2341 cagtgagaac attgcgccgc tgctgactgc acgaccggaa ttgatggtgc atgaactgac
     2401 tatggactct cttaccgaaa gcctggaatg ggccgatgtg gtggtgattg gtcccggtct
     2461 gggccagcaa gagtggggga aaaaagccct gcaaaaagtt gagaattttc gcaaaccgat
     2521 gttgtgggat gccgatgcat tgaacctgct ggcaatcaat cccgataagc gtcacaatcg
     2581 cgtgatcacg ccgcatcctg gcgaggccgc acggttgtta ggctgttccg tcgctgaaat
     2641 tgaaagtgac cgcttacatt gcgcccaacg tctggtacaa cgttatggcg gtgtagcggt
     2701 gctgaaaggt gccggaaccg tggtcgccgc ccatcctgac gctttaggca ttattgatgc
     2761 cggaaatgca ggcatggcga gcggcggcat gggcgatgtg ctctctggta ttattggcgc
     2821 attgcttggg caaaaactgt cgccgtatga tgcagcgtgc gcgggctgtg tcgcacacgg
     2881 tacggcagct gacgtactgg cggcgcgttt tggaacgcgc ggaatgctgg cgaccgatct
     2941 cttttccacg ctacagcgta ttgttaaccc ggaagtgact gataaaaacc atgatgaatc
     3001 gagtaattcc gctccctgat gagcaggcaa cattagacct gggcgagcgg gtagcgaaag
     3061 cctgcgatgg cgcaaccgta atctatctgt atggcgattt aggcgcaggt aaaaccacct
     3121 ttagccgggg ctttttacag gctctgggtc atcagggtaa tgtcaaaagc cccacttata
     3181 cgctggtcga accctatacg ctcgacaatt taatggttta tcactttgat ttgtaccgcc
     3241 ttgccgatcc cgaggagctg gagtttatgg ggatccgcga ttattttgcc aacgatgcca
     3301 tctgcctggt ggagtggcca caacaaggta caggtgttct tcctgacccg gatgtcgaaa
     3361 tacacattga ttatcaggca caaggccgtg aggcgcgcgt gagtgcggtt tcctctgcgg
     3421 gtgaattgtt gctggcgcgt ttagccggtt aacctttgaa aggtggcggg atgatgtatc
     3481 gcatcagaaa ttggttggta gcgacgttgc tgctgctgtg cgcgcacgtg ggtgccgcga
     3541 cgctctctga tattcaggtt tctaacggta atcaacaggc gcggataacg ttgagtttta
     3601 ttggcgatcc tgattatgcg tttagccatc aaagcaaacg caccgtggcg ctcgatatca
     3661 aacaaacggg cgtgattcag ggactgccat tgttgttcag cggtaataat ctggtgaagg
     3721 cgattcgctc cggaacgcct aaagatgcac aaacgctacg gctggtggtc gatcttaccg
     3781 aaaacggtaa aaccgaagcg gtgaagcggc agaatggcag caattacact gtcgtctttn
     3841 nnnnnnnnng tctttacgat taacgccgat gcgccgccac cgcctcctcc gccgcctgtg
     3901 gttgcgaaac gcgttgaaac gcctgcggtt gtcgcaccgc gcgtcagtga accagcgcgc
     3961 aatccgttta aaactgaaag taaccgcact acgggtgtta tcagcagtaa tacggtaacg
     4021 cgaccggctg cgcgcgcgac ggctaacact ggcgataaaa ttatcatcgc tattgatgcc
     4081 ggacacggcg gtcaggaccc tggcgctatc ggccccggtg gtacgcggga gaaaaatgtc
     4141 accatcgcca tcgcgcgtaa attgcgtact ttgctcaatg acgatccgat gtttaagggc
     4201 gttttaaccc gtgacgggga ttattttatc tcggtgatgg ggcgcagtga tgtggcacgt
     4261 aagcaaaacg ccaatttcct tgtgtcgatt catgccgatg ccgcaccaaa ccgtagtgcc
     4321 accggtgcct ctgtatgggt gctctctaac cgtcgcgcca acagcgaaat ggcaagctgg
     4381 ctggagcagc acgagaaaca gtcggagcta ctgggcggag cgggcgatgt gctggcgtac
     4441 agtcagtctg acccctattt aagccaggcg gtgctggatt tacagttcgg tcattcccag
     4501 cgggtagggt atgatgtagc gaccagtatg atcaatcagt tgcaacgcat tggcgaaata
     4561 cataaacgcc gaccagaaca cgcgagtctc ggcgttctgc gttcgccgga tattccgtca
     4621 gtactggtcg aaaccggttt tatcagtaac aacagcgaag aacgtttgct ggcgagcgac
     4681 gattaccaac aacagctggc agaagccatt tacaaaggcc tgcgcaatta cttccttgcg
     4741 catccgatgc aatctgcgcc gcagggtgca acggcacaaa cggccagtac ggtgacgacg
     4801 ccagatcgca cgctgccaaa ctaaggacga ttgatgccaa ttcaggtctt accgccacaa
     4861 ctggcgaacc agattgccgc aggtgaggtg gtcgagnnnn nnnnnngcga cctgcgtcgg
     4921 tagtcaaaga actggtggaa aacagcctcg atgcaggtgc gacgcgtatc gatattgata
     4981 tcgaacgcgg tggggcgaaa cttattcgca ttcgtgataa cggctgcggt atcaaaaaag
     5041 acgagctggc gctggcgctg gcgcgtcatg ccaccagtaa aatcgcctct ctggacgatc
     5101 tcgaagccat tatcagtctg ggctttcgcg gtgaggcgct ggcgagtatc agttcggttt
     5161 cccgcctgac gctcacttca cgcaccgcag aacagcagga agcctggcag gcctatgccg
     5221 aagggcgcga tatggacgtg acggttaaac cggcggcgca tccggtggga acgacgctgg
     5281 aggtgctgga cctgttctac aacaccccgg cgcggcgcaa attcctgcgc accgagaaaa
     5341 ccgaatttaa ccatatcgat gaaatcatcc gccgcattgc gctggcgcgt ttcgacgtca
     5401 ctatcaacct gtcgcataac ggcaaaattg tacgccagta ccgcgcagtg ccggaaggcg
     5461 ggcaaaaaga acggcgctta ggcgcgattt gcggcaccgc ttttctcgaa caagcgctgg
     5521 cgattgaatg gcaacacggc gatctcacgc tgcgcggctg ggtggccgat ccaaatcaca
     5581 ctacgcccgc actggccgaa attcagtatt gctacgtgaa cggtcgcatg atgcgcgatc
     5641 gcctgatcaa tcacgcgatc cgccaggcct gcgaagacaa actgggggcc gatcagcaac
     5701 cggcatttgt gttgtatctg gagatcgacc cgcatcaggt ggacgtcaac gtgcaccctg
     5761 ccaaacacga agtgcgtttc catcagtcgc gtctggtgca tgactttatc tatcagggcg
     5821 tgctgagcgt gctacaacag caactggaaa caccgctacc gctggacgat gaaccacaac
     5881 ctgcaccgcg tgccattccg gaaaatcgcg tggcggcggg gcgcaatcac tttgctgaac
     5941 cggcagttcg tgagccggta gctccgcgct actctcctgc gcccgcctca ggcagtcgtc
     6001 cggctgcccc ctggccgaat gcgcagccag gttaccagaa acagcaaggt gaagtgtatc
     6061 gccagctttt gcaaacgccc gcgccgatgc aaaaaccaaa agcgccagaa ccgcaggaac
     6121 ctgcacttgc ggcgaacagt cagagttttg gtcgggtact gactatcgtc cattccgact
     6181 gtgcgttgct tgagcgcgat ggcaacattt cacttttatc cttgccagtg gcagaacgtt
     6241 ggctgcgtca ggcacaattg acgccgggtg aagtgccagt ttgcgcccaa ccgctgctta
     6301 ttccgttgcg gctaaaagtt tctggcgaag aaaaatcggc attagaaaaa gcgcagtctg
     6361 ccctggcgga actgggtatt gatttccagt ccgatgcgca gcatgtgacc atcagggccg
     6421 tgcctttacc cttacgccaa caaaatttac aaatcttgat tcctgaactg ataggctacc
     6481 tggcgaagca gtccgtattc gaacctggca atattgcgca gtggattgca cgaaatctga
     6541 tgagcgaaca tgcgcagtgg tcaatggcac aggccataac cctgctggcg gacgtggaac
     6601 ggttatgtcc gcaacttgtg aaaacgccgc cgggtggtct gttacaatct gttgatttac
     6661 atccggcgat aaaagccctg aaagatgagt gatatcagta aggcgagcct gcctaaggcg
     6721 atttttttga tggggccgac ggcctccggt aaaacggcgt tagccattga gctgcgtaaa
     6781 attttaccag tagagttgat aagcgttgat tctgccctta tttacaaagg gatggatatc
     6841 gggacggcga agccgaacgc tgaagagtta ctcgccgcgc cgcaccgatt gctgaatatt
     6901 cgcgatccgt cgcaggctta ctcggctgct gattttcgcc gcgatgcgct ggcggaaatg
     6961 gccgatatca ccgcggcggg gcggatccca ctgttagtgg gcggtacgat gttgtatttc
     7021 aaggcattgc tggaagggtt gtcgccgcta ccgtcggcag acccggaagt acgggccaga
     7081 attgagcaac aggcggcaga gcaaggttgg gagtcattgc atcgtcaact tcaggaggta
     7141 gatccggttg cggcagca
//
LOCUS       DAWDRC010000141         7051 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5062_length_7051_cov_2.604345, whole genome shotgun sequence.
ACCESSION   DAWDRC010000141 DAWDRC010000000
VERSION     DAWDRC010000141.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5062_length_7051_cov_2.604345"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2357..2366
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3455..3464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1783 a         1779 c         1762 g         1707 t
ORIGIN      
        1 tttcagtcgc tgcagcaatc actttctggc ctgccgcagt gggcatcaga gcgtattttg
       61 cagcagataa accagcttac tcactatgag ccggtgattg gcatcatggg taaaactggt
      121 tcgggcaaga gcggcttatg taatgccctg tttgccggtg agatttcccc catcagcgat
      181 gtgatcgcct gcacacgtga ccccttacgc tttcgcctgc aggtgggaaa gcgctttatg
      241 acactggtgg atttacccgg cgtcggggag tctgagcgcc gggatacaga gtacgccgcg
      301 ctgtaccgcg aacaactccc ccggctcgat ctggtgttat ggctcattaa ggccgatgat
      361 cgggcgctgg cagtggatga gcattattat cggcaggtga ttggtgaagc ataccggcac
      421 aagatgctgt ttgttatcag tcagtccgac aaggtcgagc caatcagcgg cggtgatatg
      481 ttgtccacgg aacagaaacg aaacatcagc cgtaaaatct gccagttgca tgaactcttt
      541 cagccggtaa atccggtctg tgcggtatcg gttcgtttgc aatgggggct tagggtgacg
      601 gctgagcgca tgattcgttg tctgccacgt gaggccagca gttcggtggt atctcagctc
      661 agtgcgccgc ttcgtaccga ttccgttaat aaaaaagcct gtgacgattt tggggaaacg
      721 gtaggctcgg tactggatac aataagttcc atgcccctga taccgaaccc ggttcggaca
      781 atcattcagg ctgtacgcga caccgttgtg tcggttgccc gtaccgtctg gaatttcttc
      841 ttctgaatat tgctacttaa cccgtcgttt cctgagccct gtcgccattt gagcggcaga
      901 gcttttttgt ctttattttc catcatgagg agtctgctta tgacccgtct ggcttcgcgc
      961 tttggcgcag caaaccttat tcgtcgtgac cgtccgttaa cccgtgaaga gttgtttcgg
     1021 gtggtgccca gtgtgttcag tgaggagaag catgtgtcac gcagcgctcg gtatacctgg
     1081 ataccaacca ttaccgttct tgaaaacctg cagcgagagg gctttcagcc gttctttgcc
     1141 tgtcagactc gcgttcgtga ccctggccgc cgtgaacata caaagcacat gctgcgccta
     1201 cgatgtgagg ggcaaattac tggtaaacag gtgccagaaa tcattctgct caactcccat
     1261 gatggcacca gctcgtacca gatgctgccg ggattatttc gtgcggtatg tcagaacggg
     1321 ctcgtctgcg gcgaatcgtt tggcgaggtg cgggtgccgc acaaaggaga tgtcgtgagt
     1381 caggtgattg agggggcgta tgaagtgctg ggtatttttg accgtgtgga agagaagcgg
     1441 gatgccatgc agtcgttgat gctgccgcca cctgcacaac aggctctggc acaggctgca
     1501 ctgacgtacc gtttcggtga ggaccaccag ccggtgacag cacctcagat actctcctca
     1561 cgccgctggc aggatgagag caatgatctg tggaccacat accagcgtat tcaggagaac
     1621 ctgattaagg gcgggctcag tggtcggagt gcaaaaggcg ggcgaacgca tacccgcgcc
     1681 gtgcgtggta tcgacggaga tgtgaagctc aaccgggctc tctgggtgat ggctgaagcg
     1741 atgctgagtg ggtttcagcc ctgagattgt tatcagcatg ttaagaaagg acactccctg
     1801 tccgttttac ctccattaga ttgagtgttt tttcatcaga attcacggtg tgccgcgtcg
     1861 ggcctgcttc cggaggcaga taaaaaatag ttcattccat gtccataccc tgtccgcccc
     1921 cttctttaga atcaattcat attcaatcag ttaaccatta cggagattta agatgacaca
     1981 ggcagagcgc cgccatgacc ggctggctgt caggctgtca ctgataatca gccgcctggt
     2041 tgccggtgaa acgctgaaca tggcgcggct ggctgcagag tttggtgtgt cagtccgtac
     2101 cctgcgacgg gattttcgcg aacggctgat gtacctcgac cttgagtatc gacgggggca
     2161 atgccgcctg cgcagtaccg gcggaggcgt acagggcgag ctggatgcgc tgacctttgc
     2221 gcaccgagct gggctggccg atatttttcc ggggctggac cggcgtctgg cgggcatgct
     2281 gcttactgct ggagggatgc cgtgtctggt atggcagccg ccaagatact gatataaagg
     2341 aacgcggaga gctgctnnnn nnnnnngctc actggtgttc taccgtctcg tcagtgccat
     2401 caccgcctgc cagcgggtac tgctgctggc agagggggag cgctgtgacg ggctggcccc
     2461 gtaccgcctg atatcactcg acggcagctg gtacctcacc ggtgaactta acgggcatat
     2521 caccgtgcat ccactagcga ccatccatgc ggtaacagtt ctgaacatca cttttacccc
     2581 gcgaaaacgt ctcagccagt taaccacaca gacgggcttt atccgtgccc ttccgcactt
     2641 cagctgtatc cgcgaagtcc tgtctcctgg tccctctgag gagaagccgg acaacacact
     2701 aatttaataa cctctgttaa gaaggaaaag caatgacctg gcattacgag aaaaatggca
     2761 tacgtcacgg caatgtgacc gaagacgacc tcaccggcct gattttacgc gttgaagcga
     2821 acgctaccgc acaggtttaa tggcattaac ttaaggaaca gaaatcaatt tcaacaacgc
     2881 tcatggtaac tgtactgaat cgaaacgact atccaacgta acagccatat ttaagttagc
     2941 caggatgttt gccgatagct attaactgat atttttaaat tttcaaagag gatacctgtg
     3001 aaaatattat tgattgtcct gactctatgt ttgagcttta gcgttaaagc caccccgatg
     3061 gaggctgctg ccaactgcac cgataattgc ccttatccta gcgagctgta caaatcagac
     3121 aaagcatttg cggatgcctt agctgggaac ttgcgtgcta ttgggataaa ggacgctttt
     3181 accagaaccg gcgcgcttaa tggcactgaa tcgccggtaa gctcgtataa atataaaggc
     3241 gataccgtga ttaccggctc aatttgcgct agtgactgtt ttacccactt caatttcatc
     3301 tacaacgtcg ttaccaaaac agccgcatct gtatatatcg aaggtgataa agctactttg
     3361 atgggtaagc caccagagga cgttactaac tttctgctaa aagatgaaaa tattgccacc
     3421 acaactatcg cccctttagc accaaaggga ccatnnnnnn nnnncatctg agagtggaaa
     3481 aaattcagac aaaactatct gggaactaaa agacaaaagc gggcggctgt tgggggatgt
     3541 gtgcgctgga caaccgggta cctgcgccat cgtaatgaac acaacaaacc tcgtggcggt
     3601 agttaatcat aaatctctca acggatgcgc catgggagat ttctacgtcg taagtagacc
     3661 tgagcagttt tggagtcagt acgatacagg cacctgcagc aaagacgcct attttgctga
     3721 agggacaatg aataatggtc aatatcgtac aatcgatgtc caccttaatg gagaactggt
     3781 caaacgtttc ccaatcgaat actggtcatt ggcaaaatcc ttttccggca gtaaccgccc
     3841 gaagtggaag gaaaaggaat atggtagagg ttctatttac caaactaacg aagagccgat
     3901 ggagaaacca acttacaccc cagaggaatt ggaaaaaatg gaggctgcgc aaaaaatggg
     3961 ggggcgtggt ttttctggta cctcaaacga cattaatcaa gctgtaaatg atgccggtgg
     4021 gattatgaat tatataaaga agacaacagg aacagataaa gatcagacac aaaaataatc
     4081 cgcctcacta ccatgccagc gctctatttt tgggcgatta gaaattgaga gtcaaagtat
     4141 ttaacttagc ccctgtgctc atgttgcggg ggttaatttt acttaaagtc tatgtaaaag
     4201 cagaaattat ttttcccaac acgcaaatca ttcattcagt agcaggctga agttgttagt
     4261 tacctgaata acaacatgcc cctcaaagat tgcaagcata tccactttag cccagcgatt
     4321 cgctgttctg aaagctggat ggtctggcgg gcgtggtgct ttgaacctga tgctggtgaa
     4381 ggacttaacc gttatatccg caagtcaggc atcctcctcc tgattacacc catccttcat
     4441 ccctgcatgt cctttatttc ccatgcgcca accatcgtcg ctggcgtttt tattgacgga
     4501 gaatatccat gacgacacaa actcaacgcg aattgatgcc tgctaatgaa cctcaatttg
     4561 accttactgc aacatcagta cctgacgaac agcgtctcga tttctggccg ctgtactttg
     4621 gtgctattcc gcagtggatc acgctggaac cccgtatttt cgcctggata gatcgctttt
     4681 gtgataagta cagcggtggt atctggtcct tttacacact tagcaatggc ggcgcgttta
     4741 tggcccctga tgctgacggt ggtgataaat ggcatctgtt caacagcatg aacggcaatg
     4801 gtgcgggaat gagcgcggaa gccgcaggta tcgctgtctg cctaattgaa tacagccacc
     4861 atgcctgtcg caccgaatgc gatgccatga cgcagcacta ttaccgcctg cgggattatg
     4921 ccctgcagca ccctgaatcc agcgccattc tgcgcattat tgattgagga cactcacgat
     4981 ggaacagtca atttccccac tggctccggt actcccactg accgtacaac gcactgtgca
     5041 acatgccctg gccctgcttg accgccacct gcgcgaaact ggcgtggcgt tcacctccac
     5101 acaggccgcc cgcgactggt tgcggttgaa aatggccggg cttgagcgag aggagtttat
     5161 ggtgctttat ctcaatcagc agaaccagtt gattgcccac gaaaccctgt ttgccggttc
     5221 catcagcagc accgaggtac atccccggga ggtggtcaaa cgcgccctgt atttcaatgc
     5281 ggcagcagtg atactggcgc ataaccatcc ctccggcgag acgacgccca gccaggctga
     5341 taaagctctc acgcagcggc tggtacagat acttcaactg gtggatatcc gcgtccctga
     5401 ccatctgatt gtcggcggta ggcaaatcta ttcgttcgca gaacagggtc tgctgtgagg
     5461 aactacatga aaattatcag taagcgccgg gtaatgacga tacaccgcca gcatcctgag
     5521 tcccgaatct ttcgctactg taccggaaaa tatcagtggc acggtagcgt ctgccactac
     5581 accggcaggg aagttccgga tatcacagaa gtcctggcgg tatacgccga acgccgccag
     5641 gaccgcaaag ggccttatac ctgcctgatg agtattaccc tgaactgaca ataaaggagg
     5701 ttataaatga gcaacatcac atgggggctg cagcgggata tcacgccacg cctgggagcc
     5761 cgtctggtgc aggaggggaa ccggctgcat tatctggctg accgggccag catcaccggt
     5821 aaattcagtg acgtcgaatg ccgtaagctg gatgaaacat tcccgcactt tatcagccag
     5881 atggagtcga tgctgaccac cgatgaactg aatccccgcc atgcccactg cgtcaccctg
     5941 taccacaatg gtttcacctg cgaagccgac acccttggca gttgcggcta catatacatc
     6001 accgtttatc ccactcaacg ttaactaatt tcacgagagc aaacatgaaa actctacctg
     6061 caacaacttt gtgggcggct aaaccatgtc cgtcaccagt gatcgtatgg caaatgcttc
     6121 tttcacgcct gctggatcag cactatggcc tgacactgaa cgacacgcca ttcagtgatg
     6181 aaactgtgat taaggaacat atcgatgccg gtatctcttt agccgatgcc gttaattttc
     6241 tggtggaaaa atacgaactg gttcgcatcg atcgtaaggg gtttagctgg caaaaacagt
     6301 cgccttttat tactgctgta gacattctaa aagccagacg ggctatgagg gaaatgagaa
     6361 cttaagtagt gtcagttttc ttcccacacc ccatctcttt actgggcaaa aaaagtaaaa
     6421 atatttttag gcaccacatt aggcaccatc acaaagttga attaggattt agatcatata
     6481 attcagctaa ttacagcacc aattcagact ccgccagccc ccaatcatga ttggacggtg
     6541 taaggactac accaacaaaa acaggaagtt ataagtctca gcaggacacc gaccagacgg
     6601 tgaggagaca aaaaaggata cgcaacggag tcgcgactcc ctatgacatt aaagcccgct
     6661 gatgcgggct tttttattgg tcctgacccg ccctgaatag aattcatcca attacagtcc
     6721 agacatatac agtatccctc agctcagtat cagcttctcg acaaatggtc actgttattt
     6781 acccgttgcc agaaagagaa taatttgccc ccatagccag cctttccgga atctgtatat
     6841 tcattaatat tgttatcgat tccatcaagt aacataggtt ggatgcgatt gatcttactc
     6901 acattagtcg acacggaact cagtaagttg aactctggaa aagcagttta aatagcattt
     6961 aaacaaatgc caccagttcg aaaaagagta taagcacact taatatattg ttttaattat
     7021 tcatttcctt tcaacccaat tcttatccct c
//
LOCUS       DAWDRC010000142         7047 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5068_length_7047_cov_2.165618, whole genome shotgun sequence.
ACCESSION   DAWDRC010000142 DAWDRC010000000
VERSION     DAWDRC010000142.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5068_length_7047_cov_2.165618"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    911..920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1696..1705
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2230..2239
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4501..4510
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6658..6667
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2042 a         1387 c         1705 g         1863 t
ORIGIN      
        1 tttagccaac tggacaccgt cgaactgaat cccgttgtca aacgtgcgcg cgaaaaagtc
       61 gaacacgatt ttaaccagca ggtgatcaat cgagaactcg ccagcttgct gcaggcttta
      121 tagaggagaa cgtatgccat ttaaaaaact ctccagacgc aacttcctga cggcaagttc
      181 tgtgcttgcc ttcctccata cgcctttcgc ccgcgcactt cccgctcaac aaagcgttaa
      241 cattaacgac tacaaccctc acgactggat cgcctcattt aaacaagcct tcagcgaagg
      301 gcaaacggtt gtcgtgcctg gcggattggt ttgtgacaat atcaacaccg gcatctttat
      361 ccctcccggt aaaacgttac acattcttgg aagcttgcgc ggcaacggca gagggcgatt
      421 tgtcttacag gacggcagcc aggtgacagg ggaggagggc ggcagcatgc ataacatcac
      481 cctggatgtg cgcggctctg actgcaccat caaagggctg gcgatgagcg gctttggtcc
      541 ggtaacgcag atttatatcg gcggcaaaaa caaacgggtc atgcgaaacc tgaccattga
      601 taaccttact gtcagccacg ctaattacgc catcttacgc cagggatttc ataaccagat
      661 aatcggtgcc aacatcacca actgtaagtt cagtgactta cagggcgacg ctattgaatg
      721 gaacgtggcg attaacgaca gtgatatttt gatatccgac catctcattg agcgcattaa
      781 ctgtaccaac ggcaaaatca actggggcat cggcataggc cttgcgggaa gtacctacga
      841 taataattat ccggaaaacc aggcagtgaa aaactttgtc gtggcgaata tcacgggatc
      901 agattgtcgg nnnnnnnnnn tcggcaactg atccatgttg aaaatggtaa acattttgtt
      961 atccgtaata tcaaagctcg taatatcacg ccggatttca gtaagaaagc aggtattgat
     1021 aacgccacag ttgctattta tggttgtgac aactttgtga ttgataatat tgaaatgatt
     1081 aatagtgccg ggatgttaat cggctatggg gtaattaaag gcaaatatct ctcgataccg
     1141 cagaatttcc gagtgaatga tattcaactg gataatactc atcttgctta taaattgcgc
     1201 ggcattcaaa tctccgccgg gaatgctgtc tcctttgtgg cgctgactaa cattgagatg
     1261 aagcgtgcgt cgctggagtt acacaacaaa ccgcaacatc tttttatgcg aaatatcaat
     1321 gtgatgcagg aatcctcagt tggacccgca ttgagcatga actttgacat gcgcaaagac
     1381 gttcgaggcg tgtttatggc gaaaaaagaa acactgctgt ctcttgcaaa tgttcatgcg
     1441 gtgaatgaaa aagggcaaag ctccgtcgat atcgacagga ttaatcacca tattgttaat
     1501 gtggaaaaga ttaactttag attgccggaa cggagagagt agatttgcga ccattcctgg
     1561 aaaaatggag ccatacttag gaacaatgct actgcaatcc acaacgaagc ggcgtaacat
     1621 cacaagtaat tcagtaatca attcagggta attgatgctg gcgaaaaaaa tcgaacaagc
     1681 tataattcag caaccnnnnn nnnnnggatg aaataatgac gaatttaaaa tcagttatta
     1741 ctgtagcggg gctagggatg catatgttgc ctgccactaa ggcgattccc aaggagatgc
     1801 taccgatcgt cgacaagcca atgattcagt acattgttga cgagattgtg gctgcaggga
     1861 tcaaagaaat cctcctggta actcacgcgt ccaaaaacgc ggtcgaaaac cacttcgaca
     1921 cctcttatga attagaatct cttcttgaac tgcgcgtgaa gcgtcaactg ttggcggaag
     1981 tacagtccat ctgtccgccg ggcgtgacca ttatgaacgt gcgtcagggc gaacctttag
     2041 gtttaggcca ctccattttg tgtgcacgac ccgccattgg cgataacccg tttgtcgttg
     2101 tgctgccgga tgttgttatc gacgacgcca gtgccgatcc gctgcgttac aacctggccg
     2161 caatgattgc gcgctttaac gaaactggcc gcagccaggt tctggcaaaa cgcatgccgg
     2221 gcgagctctn nnnnnnnnng actcagacat catggctgta gggcgttatg tgctttctgc
     2281 cgatatttgg cctgaactgg agcgtactca acctggagca tggggacgta ttcagttgac
     2341 tgatgccatt gctgagttgg caaaaaaaca agcagttgac gcaatgctga tgactgggga
     2401 cagttacgac tgcggaaaga aaatgggtta tatgcaagcg tttgtgaagt atgggctgcg
     2461 taacctaaaa gaaggggcga agttccgtaa agggattgag aagctgttaa gcgaataatg
     2521 aaaatctgac cggatgtaac ggttgataag aaaattataa cggcagtgaa gattagcggc
     2581 gaaagtaatt tgttgcgaat tttcctgccg ttgttttata taaacaatca gaataacaac
     2641 gacttagcaa taggattttc gtcaaagttt tccaggattt tccttgtttc cagagcggat
     2701 tggtaagaca attagcattt gaatttttcg ggtttagcgc gagtgggtaa cgctcgtcac
     2761 atcgtagaca tgcatgcagt gctctggtag ctgtaaagcc aggggcggta gcgtgcatta
     2821 atacctctat taatcaattt gagagccgct tattttacag catgctctga agtaatatgg
     2881 aataaattaa gtgaaaatac ttgttactgg tggcgcagga tttattggtt ctgctgtagt
     2941 tcgtcacatt ataaataata cgcaggatag tgttgttaat gtcgataaat taacgtacgc
     3001 cggaaacctg gaatcacttg cagatgcttc tgattctgaa cgctatgttt ttgaacatgc
     3061 ggatatttgt gatgcagctg caatggcacg gatttttgct cagcatcagc cggatgcagt
     3121 gatgcacctg gcggctgaga gccacgttga ccgctcaata actggcccgg cggcattcat
     3181 tgaaactaat attgtaggta cttacgtact tttagaagca gcgcgcaatt attggtctgg
     3241 tctggatgat gaaaagaaaa aaaacttccg ctttcatcat atttctactg atgaagtgta
     3301 tggtgactta ccccatccgg atgaagtaaa tagcaatgaa acgttgccgc tatttacgga
     3361 aacgacagca tacgcgccaa gtagtccata ttctgcttct aaagcgtcaa gcgatcattt
     3421 ggttcgcgca tggaaacgta cttatggttt accgacaatt gtgactaatt gctcgaacaa
     3481 ctatggtcct tatcatttcc cggaaaagct tattccactg gttattctta atgcactgga
     3541 aggtaaggca ttacctattt atggtaaagg ggatcagatc cgcgactggt tgtatgtaga
     3601 ggatcatgct cgtgcgttat ataccgtagt tactgaaggt caagcgggtg aaacctataa
     3661 cattggcgga cacaacgaaa agaaaaacat cgatgttgtg ctgactattt gtgatttgtt
     3721 ggacgagata gtcccgaaag agaaatctta tcgtgagcaa attacttatg ttgctgatcg
     3781 cccagggcat gatcgccgtt atgcgatcga tgccgataaa attagccgtg aattgggctg
     3841 gaaaccgcaa gaaacgtttg agagcgggat tcgaaagacg gtggaatggt acctgtccaa
     3901 tacaaaatgg gttgataatg tgaaaagtgg cgcctatcaa tcgtggattg aacagaacta
     3961 tgaggaccgc cagtaatgaa tatcctccta ttcggcaaaa cagggcaggt aggttgggaa
     4021 ctacagcgtg ctctggcacc gctgggtaat ctgattgctc ttgatgttca ctccactgat
     4081 tattgtggtg attttagtaa tcccgaaggt gtagctgaaa ctgtcaaaaa aattcgccct
     4141 gatgttattg ttaatgcggc tgctcacact gcagtagaca aagcggaatc agaaccggaa
     4201 tttgcacaat tacttaacgc gacaagtgtc gaagcgattg caaaagcagc caatgaagtc
     4261 ggcgcttggg ttattcacta ctctactgac tacgtatttc cgggaaccgg tgaaataccg
     4321 tggcaggaga cggatgctac agcacctttg aatgtttatg gtgaaactaa actagccgga
     4381 gaaaaagcgt tacagaaaca ttgcgcgaag catattattt tccgtaccag ctgggtctat
     4441 gcaggtaaag gaaataactt cgccaaaaca atgttgcgtc tggcaaaaga gcgcgaagaa
     4501 nnnnnnnnnn aagaactggc tgtgataaac gatcagtttg gcgcaccaac aggtgccgaa
     4561 ttgctggctg attgcaccgc tcatgccatt cgcgtagcag tagataaacc agaagttgct
     4621 ggtttgtacc atctggttgc tggcggtaca acaacctggc acgattatgc cgctctggta
     4681 tttgaagaag cgcgcaaagc agggattaac cttgctctta acaaacttaa cgccgtgcca
     4741 acaacggcct atcccacacc agcccgtcga ccccataact ctcgcctcaa tacagaaaag
     4801 tttcagcaga actttgcgct tgtcttgcct gactggcagg tgggcgtgaa acgaatgctc
     4861 aacgaattat ttacgactac ggcaatttaa caaatttttg catctcgctc atgatgccag
     4921 agcgggatga attaaaagga atggtgaaat gaaaacgcgt aaaggtatta ttctggctgg
     4981 tggttccggc actcgtcttt atcctgtgac gatggcagta agtaaacaac tgctgccgat
     5041 ttatgataaa cctatgatct attacccact ctcaacactg atgctggcgg gtattcgcga
     5101 tattcttatt atcagtacgc cacaggatac accgcgtttc caacaattgt tgggggacgg
     5161 gagccagtgg gggcttaatc tacagtataa agtacaaccg agtccggatg gcctggcaca
     5221 agcgtttatc attggtgaag actttattgg tggcgatgat tgtgcactcg tgcttggaga
     5281 taatatcttc tatggacacg acttgccaaa attaatggac gctgcggtta acaaagaaag
     5341 cggtgcaacg gtatttgctt atcacgttaa tgatcctgaa cgctatggtg tcgtggagtt
     5401 tgataataac ggtacggcaa ttagcctgga agaaaaacca ctggaaccaa aaagtaacta
     5461 tgcggttact gggctttatt tctatgacaa tgacgtcgtg gaaatggcga aaaaccttaa
     5521 gccttctgcc cgtggcgaac tggaaattac cgatattaac cgtatttata tggaacaagg
     5581 acgtttgtct gtcgctatga tggggcgtgg ttatgcatgg ctggatacag ggacgcatca
     5641 aagccttatt gaagcaagta acttcattgc caccattgaa gagcgtcagg gattaaaagt
     5701 atcttgcccg gaagagattg cttatcgtaa agggtttatt gatgctgagc aggtaaaagt
     5761 attagccgaa ccgcttatca agaatcaata tggtcaatat ttgctgaaaa tgatcagcga
     5821 atagtatgtg ggaactcaat gatggatatt aaattaatct ctttgcaaaa acatggggat
     5881 gagcgtggtg cattaattgc tcttgaagag caacgaaata tacctttcga agtcaaaaga
     5941 atatattaca tactcgagac tcttaaggga gtaagacgcg gatttcatgc gcacaaggtt
     6001 actcgtcagt tagctattgt agtcaaggga gcttgtaaat ttcatctgga taatggtaaa
     6061 gaaacaaagc aggtggaact taatgatcca acaattgcgt tgctgataga accctatata
     6121 tggcatgaaa tgtatgattt tagtgatgat tgtgtgctgc ttgtaattgc ggatgatttc
     6181 tataaagagt ctgattatat acgcaattat gatgatttta ttagaagagt aaattcaatt
     6241 gagaattcat aagctaagtg acgtccagac aacatcaatt ggtgatggaa caactatctg
     6301 gcagtttgtt gtgatactaa aaggtgctgt aattggtaat aattgcaata tctgtgcaaa
     6361 taccttgatt gaaaataacg ttgtaattgg taacaatgtc acagtcaaaa gcggtgtgta
     6421 tatttgggat ggcgttaaaa tagaggataa tgtttttatt ggtccttgtg tagcatttac
     6481 aaatgataag tatcctcgct ctaaagtcta tcctgatgaa tttttgcaca caataatacg
     6541 caaaggagca tcaataggtg ctaatgcaac catcctgcca ggaattgaaa ttggtgaaaa
     6601 agcaatcgtt ggtgcgggga gtgtcgtaac caaaaatgta ccgccatgcg caatagtnnn
     6661 nnnnnnnagt aggtaatcca gctcgattta ttaaatgggt agaggataat gaataaaatt
     6721 gattttttag atctttttgc aattaaccag cgacagcaca aagaattagt ttctgcgttt
     6781 agtagggtgc tagattctgg ttggtatatc atgggcgaag aacttgagca gttcgagaaa
     6841 gagttcgcag aatactgtgg agttaagtat tgtattggtg tagcaaatgg ccttgatgcg
     6901 ttgatactag tattgagggc atggaaagaa ctcggctatc tagaggacgg tgacgaggta
     6961 ttagtaccgg caaatacata tattgcttct attcttgcta taacagagaa caaacttgtt
     7021 cctgttcttg ttgaaccaga tatagaa
//
LOCUS       DAWDRC010000143         7010 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5092_length_7010_cov_2.143494, whole genome shotgun sequence.
ACCESSION   DAWDRC010000143 DAWDRC010000000
VERSION     DAWDRC010000143.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5092_length_7010_cov_2.143494"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2636..2645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4195..4204
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6624..6633
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1737 a         1759 c         1718 g         1766 t
ORIGIN      
        1 agcgatatac gctatgcgaa aatgcagatg gcaatgagat ccgctgcttt catctccatt
       61 aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc
      121 gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta
      181 ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga ttggtagcga
      241 ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg tttatgcgcg
      301 cggcacgcaa agttagcccg tcgggctgga taaaaaatct ggcggatatc ctggcttatg
      361 tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca gactggcatc
      421 agattatggc ggcagtcagt tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg
      481 gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag caggtaaaag
      541 cctgaccctt cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat
      601 aaaccattca aataacgttc aagcgcaaca cgcgaactaa agccatgcgg gataccgtaa
      661 tgctcatgac gttcacccgc taaatagagc ggaaattgct ggtaatgatc gcaagtttta
      721 atatcggaag cgggttcagc cagtgttgaa ctacgttctt ttaatgttgg atgaatcacc
      781 agggcggtac gtcccattcg cgcttcgcga ttaacataga cataattttc cccacggcgg
      841 taaccatacg ctttctgggt taccacatcg acagtaaaac cgactttttc aagtacgcgc
      901 gccacctcgt ctggtcgtaa atacatattt gatcctcgtt attatcaacc gcgggcctac
      961 cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc gatgaacctc
     1021 agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg ggatcatatg
     1081 tttaaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca gaatgatgtc
     1141 aatcaattag ctgacagtct cgaatctgta ttgaagtcct ggggcagcga cgccaaaggg
     1201 gaagccgaag ccgctcgtag taaagcgcag gctctactga aagaaaccag ggcgcgaatg
     1261 catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg ttggctgcgc ggattctttt
     1321 gttcgtgaaa gaccctggtg tagtgtgggt acagcggcag cggtgggtat ttttatcggc
     1381 gcactgttaa gcatgcgcaa atcgtagtgc aaaaatgata ataaatacgc gtctttgacc
     1441 ccgaagcctg gcttcggggt tttttagctt aaggctttac gtaattgttg tgctacctgt
     1501 cgcaccatac cttcgttaac gatattagta aacgaaagca gtaatccacc ttccccactt
     1561 gaacggatac gccagtcgct aagtgcctgc accgcaaggc ctgcagcatt ggctttacgc
     1621 gcatgggcaa tatcatcgcc tatcattctg atcaccatct ggataccacc tttctgcggc
     1681 acaacctgaa atccttgctg cgtcaacgct tgctcaatcc attgccgacg ttgagcataa
     1741 tgctgacgca ttttcttcag atgccgccag aaatgcccct cgcgaagaaa gtctgccagt
     1801 gtgttctgcc atagaacagg taccgcacat ggagccagtg acgcctggtg gcggaattgt
     1861 gcaatttgct tcaccggcac caccagccac gcacagcgca atgcaggaaa tagcgctttg
     1921 ctgaatgttc cggcataaat tacccgctgc ggtgcgtcga gactttttaa cgccggtaac
     1981 ggcttaccgt gatagcgaaa ctcactgtca taatcatctt caataatcca tgcctgacta
     2041 cgatctgccc attccagtat ctgatgcctg cgcgctaaag agagcgccac acccagcgga
     2101 ctttggtgtg ctggtgttat cagggcaaaa cgcgcatcag gataattttg tattccgctt
     2161 gtgatatcca gtccattgtc atcaaccggc acaggcaaaa tttccacatc tggtctgctt
     2221 ttttaggtaa tttatggctc tctttagcag tccattatag tgctacattg cgggcgttaa
     2281 taagaggaga aactgtatga ctacattacg tcagccttac tacgaactta gcccggcagt
     2341 gtataacgca cttgtgcagg ccaaaacggc actggaaaat agcacgctgg ataccacgtt
     2401 gatggagtta gtttatttgc gcgtctcgca aatcaacggc tgcgcatttt gtctggagat
     2461 gcacagcaaa gcattgcgca aatccggcgt gccacagcac aaactggacg ccctggcagg
     2521 ttggcgcgta agccatcatt ttgatgaacg cgagcgggcg gcgctggcat gggcggaatc
     2581 ggtaaccgaa attgccagaa cccatgcgga agacgaggtt tatcagcctt tgcttnnnnn
     2641 nnnnnttgct tgagcatttc agcgcagcag agatcagcga cttaacgttt gccatcgggc
     2701 tgatgaattg ttttaaccgt ctggccgttt ccatgcggat gtaacttctt gatgccgggt
     2761 attcgcccgg caaaaaaaca caaaatcccc taccccgttg agccattcat cggtggcgta
     2821 atcaatctaa aattcccata tcttgtatgg ttgaatcttt tttcaactac atctagtatc
     2881 tctgtatcaa cagagagaca acccgacgcg tatcatcgcg ccgtatcttc attttaaacg
     2941 gaaatacgaa tcatgcgcat tactatttac actcgtaacg attgcgttca gtgccacgcc
     3001 accaaacggg cgatggaaaa ccggggcttt gattttgaaa tgattaatgt cgatcgcgtt
     3061 cctgaagcgg cagaagcgct gcgtgctcag ggctttcgtc agttgccggt tgtgattgca
     3121 ggcgatctta gctggtctgg tttccgcccg gacatgatca atcgtctgca tccagcgcca
     3181 cacgcggcca gtgcatgagc cagctcgtct acttttccag cagctccgaa aatacgcagc
     3241 gttttatcga acgtttaggg ctgcccgcgg tgcgcatccc gctcaatgag cgggaacgga
     3301 ttcaggtaga cgagccttac atcctgatcg tgccctctta cggcggcggc ggtacggctg
     3361 gcgcggtgcc acgacaggtc attcgctttt taaacgacga gcataaccgg gcgttgctgc
     3421 gcggcgttat tgcttcgggt aatcgcaact ttggtgaggc atatggccgc gccggagatg
     3481 tgattgcccg gaaatgcggc gtgccgtggc tgtaccgctt tgaactcatg ggtacgcaaa
     3541 gcgatatcga aaacgttcgt aaaggagtaa ccgaattttg gcaacgacaa ccgcagaacg
     3601 cctgacgcag gaaacgatgg attatcacgc tctgaatgcg atgcttaacc tttacgatag
     3661 cgcaggtcgc attcagttcg ataaagacag ccaggcagta gacgccttta tgacgacgca
     3721 tgtgcgtccg aacagtgtgg ccttcagtag ccagcagcag cgcctgaact ggctggttaa
     3781 cgaaggttac tatgatgaaa gtgtccttaa tcgctactct cgcgattttg tcattacgct
     3841 gtttgcccac gcgcacgcca gcggttttcg tttccagaca ttcctcggag catggaagtt
     3901 ttacaccagc tatacgttga agacattcga cggtaagcgt tatctggaag attttgccga
     3961 tcgcgtcacg atggtggcgc tgacgctggc acaaggcaat gaaacactgg cgacgcagct
     4021 cactgatgag atgctgtcag gacgctttca gccagccacg ccaacattcc tcaactgcgg
     4081 taagcagcag cgcggcgaac tggtttcctg ttttctgctg cgtattgaag acaatatgga
     4141 gtcgattggc cgggcagtga attccgcact acaactgtcg aaacgcggcg gcggnnnnnn
     4201 nnnncctgac gcgcgccaat taaacgtatt gaaaatcaat cttctggcgt gattccggtg
     4261 atgaaaatgc tggaagacgc attttcctat gccaaccaac tcggcgcgcg tcagggggct
     4321 ggggcagtct atttacatgc tcatcatccc gatattctgc gttttctcga tacaaaacgg
     4381 gaaaatgccg acgaaaaaat ccgcattaaa acactgtcgc tgggcgtggt gatcccagat
     4441 atcactttcc atctggcaaa agagaatgcg cagatggcgc tgttttcgcc ttatgacgta
     4501 gagcgagttt atggcaagcc atttgccgat gtcgccatca gtgaacacta tgacgaactg
     4561 gttgccgatg aacgcattcg caaaaaatac ctcaacgccc gtgatttctt ccagcgactg
     4621 gcagagatcc agtttgagtc tggctatccg tacatcatgt atgaagacac ggttaaccgt
     4681 gctaacccta tcgccggacg cataaatatg agcaatctct gctcagaaat tttgcaggtt
     4741 aacagcgcct cagagtatga cgagaatctc gactatgccc gtacaggcca tgatatttcc
     4801 tgcaatttag gttcgttgaa tattgcccac accatggatt cccccgactt tgcccgcacg
     4861 gtagagactg ccgtacgcgg tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg
     4921 ccgtccatcg aagccggaaa tgccgcctcc cacgccatcg ggccggggca gatgaattta
     4981 cacggttatc tggcgcgaga aggcatcgct tatgggtcgc cggaagcact ggatttcacc
     5041 aatctctatt tctataccat cacctggcac gcactgcgta cctcgatgtt gctggcgcgc
     5101 gaacgcggtg aaaccttcgc cgggttcaaa cagtcacgct atgccagtgg tgaatatttc
     5161 agccaatatc tgcaaagtaa ctggcagccg aaaacggcga aagttggcga actgtttgcc
     5221 cgtagcggta ttacgttacc tacccgtgag atgtgggcgc agctgcgcga cgacgtgatg
     5281 cgctatggta tctacaacca gaatctccag gcggtgccgc caaccggttc tatctcttat
     5341 atcaaccatg ccacgtcgag tattcatccg attgtggcga aagtagagat acgcaaagag
     5401 ggcaaaacag gacgcgttta ctaccctgcc ccgtttatga ctaacgaaaa tctggcgcta
     5461 tatcaggatg cttacgaaat cggcgcagaa aagatcatcg acacctacgc ggaagcgact
     5521 cgccatgtcg atcaggggct gtcgctgacg ctgtttttcc ccgataccgc caccactcgc
     5581 gatatcaaca aagcgcagat ttacgcctgg cgcaagggta tcaaaacgct ctattacatt
     5641 cgcctgcgtc agatggcgct ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt
     5701 taaggaatat ctatgaaact ctcacgtatc agcgccatca actggaacaa gatatctgac
     5761 gataaagatc tggaggtgtg gaatcgcctg accagcaatt tctggctacc agaaaaggtg
     5821 ccgctgtcga acgatattcc tgcatggcag acattaagtg tcgtagaaca acaactgacg
     5881 atgcgcgttt ttactggcct gacgctgctc gacacgctgc aaaatgttat cggcgcgcct
     5941 tcgctgttgc ccgatacact cacgcctcat gaagaagcgg tattatcgaa tatcagcttt
     6001 atggaagcgg ttcatgcccg ctcttacagt tcgattttct cgacgctgtg ccagaccaaa
     6061 gatgtcgatg ccgcctacgc ctggagtgaa gaaaacgcac cgttgcagcg aaaagctcag
     6121 attattcagc aacattatcg cggggatgat ccgctgaaaa agaaaatcgc cagtgtgttt
     6181 cttgaatctt ttttgttcta ttccggtttc tggctgccga tgtattttgc cagccgcgga
     6241 aagctaacca ataccgcaga cctgatccgt ctgattatcc gcgatgaagc agtccacggt
     6301 tactacatcg gctataaata tcagaaaaac ctggaaaaaa tctccctggc acaacgtgaa
     6361 gagttgaaga gtttcgcctt cgatttgttg ctggaacttt atgacaacga gttgcaatac
     6421 accgatgagc tgtacgccga aaccccgtgg gctgacgatg tgaaagcgtt tctctgttac
     6481 aacgccaata aggcgttgat gaatctgggc tacgaaccgt tatttcccgc agaaatggcg
     6541 gaagtgaatc cggcaatcct cgccgcgctt tcgccgaatg ccgatgaaaa tcacgatttc
     6601 ttttccggtt caggctcttc atannnnnnn nnnttcatat gtgatgggaa aagcggtcga
     6661 aacagaagat gaagactgga atttctgagg gtgttatttt tcaaaaatat cactacccgc
     6721 agcagggaaa taattcccgc caaatagctt tttatcacga aaatattttg tggtgatcta
     6781 cactgacact ctgttgcatt attcgcctga aaccacaata ttcaggcgtt ttttcgctat
     6841 ctttgacaaa aaatatcaac ttttctcgat ttgctctcag cccttatatc atgggaaatt
     6901 ccggcgattt gctcacatca atattcatgc cacatttgcc atcaggggtt gcctcagatt
     6961 ctcagtatgt tagggtagaa aaaagtgact atttccattg ggtaatgtat
//
LOCUS       DAWDRC010000144         6998 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5104_length_6998_cov_2.777042, whole genome shotgun sequence.
ACCESSION   DAWDRC010000144 DAWDRC010000000
VERSION     DAWDRC010000144.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5104_length_6998_cov_2.777042"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1058..1067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1836 a         1593 c         1746 g         1813 t
ORIGIN      
        1 gatgaattct ctttgcagga tgaagtacct gttgaacatg acgctacagc ggaaaaagtc
       61 gaaaaagaga acagtttcca gcctccgttg attattgccg caggcgagac cagcgaggcc
      121 ggttataccc tacaacgcct cgaccgccag acgcgccgta ttggcgttat taacaatgac
      181 agcattccgc tgattattaa tgatattgaa caggtttgta gcgccagtgg ctgtggctac
      241 agaggcatga gtgggaaaca gcccttccgg cgtgcactat taggtgggcc attttacgtt
      301 actaacatcg tgcccaccgt tctagagtat tgtcaggact ttaccagtga tgaaggcaaa
      361 gagggcgtcg gaccagattc gttgccagga cgaggtcgtc gactcattac ctttacggac
      421 agtcgacaag gtacagcgcg aatggcggtg cgtatgcaac aagaagcaga acgcagtcgc
      481 ttacgcggaa gcgtagtcga aattctgagc tggcatcaac gaacgcaaac gcccacggcg
      541 ccgaacgcta atgccgatct ggaaaaatta gcgaccaggg taaagcaagc ccgtgagcag
      601 gcagaagaat atcgaagctg gggattgccg gatcaagcga aattatcaga agcgcaggcc
      661 gagcagcttg aacaggctta tcagtctgca atcggcggaa aagcagcaac caccttggta
      721 tcctgcacct ggacagaaat ggttaacgag cttaaagata aagccgatat ccgtggcccg
      781 gttctgaaat ataaccacta tttgcagcct gaagtgttta atgacaacgg cgggccacta
      841 aagctttcgg aaatgttgtt attccgggaa ttcatgcgtc gccctaaacg gactaacagc
      901 ctggaaacac aagggctggt tcaggttggt tacctggggc tggagaaaat acataagatc
      961 cctctgcact ggcaggaaaa aggattaacg ctggatgact ggcgcgattt tctcaaagtt
     1021 acgctggatc attacgttcg tgagagcaac ttcacacnnn nnnnnnnatg agctgaaaaa
     1081 ctggatcggt agccgttttt catcaaaatt cgtccgtaac ccggaatcaa aagatcctga
     1141 agataatcag aacagacgct ggcctcaaat tcgtaatggc aacgtctccc atcgtttagc
     1201 gaagttactg atgctggggg ctggattcaa aaccgttaat acggcgacta ttgatattat
     1261 caatacatgg ctgaaagaag catgggccca acttaccgga ccgcttgcag tactgaaacc
     1321 cgatggcaac cgtttttatt taccgaaaga gcatatgact ttttctttaa tcacggatgc
     1381 ctggatttgc ccggtaacca ataaaatcct ggatacggct tttaaaggct taacccctta
     1441 tctgcctacc catatttcgt tcgagcatct tacccaggcg caatatgaca cttttgtcgc
     1501 gcagaaagtc accatgccgg aaatctggaa gctggatcgt tcacaggaag actacgcaga
     1561 gggtctggca aaagcacgcg actgggtcag caaagatccg ctgatcgcac aattgcggtc
     1621 ggaaaacgtc tggacagaca ttaacgatcg tgtcgtggaa ggcggtttct actatcgcac
     1681 tgccgagcac tctgcccagc agtcgtcaga acgcctgcaa agctatgaaa agatgttcca
     1741 gaacgggcaa ctaaatgttc tcaactgttc caccaccatg gaaatggggg ttgatatcgg
     1801 gggcattact gcggtggtaa tgaacaacgt tccgccacat ccggccaact atttacagcg
     1861 cgccgggcga gccgggcgta gtaaagaatc acgcgcgatc tcttataccc tatgcaaagg
     1921 taacccacac gaccaacagg tttttgccaa cccgctctgg ccgtttgaaa ccatgatccc
     1981 ggcccccatg gtcgcgatga gctccgcccg actggtgcaa cgtcacgtca atgcattatt
     2041 actgtccgac tttttatgta acgttattgg cgaaaccgat aaagaaaaaa ccagtctcga
     2101 tagcctctgg tttttcggtg aagatgacgg gcagtcaaaa tgcgaacgct ttaaaatctg
     2161 gctggaacgt ccggttcttg atatcgacac ggcgctggaa cggctggtga aaggtacggc
     2221 gttgcatggt gcaagagccg aatatctgcg ggataaaaca atcaacgcga tcaccttcct
     2281 gcagcagcgg tggctctccg tttatcgcga tctcgtgaca caggagcgtg agtcgcagcc
     2341 gcaaacgcct tatcgtaagc gcatagagct ggagaagaaa cggcactgtg gcgagtatct
     2401 gctgcgcgat ctggctgcca gaaccttcct gccgggatac ggtttcccga cagatgtagt
     2461 gacgtttgat aacttcacga tggaagatta tatccgcgaa aaatcgcaga aaagtcgtga
     2521 taaaaaggac cgtgaagata acgtctcccg ttataaagga ttaccttccc gtaacctcgg
     2581 tgttgccatt cgtgaatatg caccgggagc agagatcatt cttgatggcc gtgtcttccg
     2641 ctctgccggt gtctcactgc actggcacaa cattaatgct gataccaatg aagcccagcg
     2701 tctggattgc gcctggcgct gtcacaaatg cgggaccatt ggctatgaag aggggatgag
     2761 cagttccggg atgctctttt gctccaatag cgcctgcggc gaaaaaataa tcatggataa
     2821 tcgccgtcag gtgctgcagc ctgccgggtt tgttaccgat gcacatgctc cggtaacgaa
     2881 caatattgaa accatgaaat tcgtcccggt tgtccccgcg tgggtctttg ttaaagcaga
     2941 gcccgtaccg ttgcctaacc cgctaatggg ttatatggct tctggggctg atgggcatgt
     3001 tttccagcaa agtctggggg aaggtggaca cggttatgca ttgtgtctga gctgtggccg
     3061 tgcggagtcg atgctgaatg aaaatgatgc tccgaaaagt atggaagctc actatccacc
     3121 gcgtccgggt aaagcggacc gcgacagcca gaatcatcgt ctgatttgcc caggttcgac
     3181 ggcactcgtg aagaaagtca ccctgggcgc actggcgcgc accgatgtct ttgaaatggt
     3241 tctgcgcaaa ccacaaaatg gcgagtatct gccagataac acagaagagg ggcggattgt
     3301 cgccatgact ctggcggtta ctctgcgtca ggcgctggcg ggcgttctgg gtatctccac
     3361 cgcagaactc ggctattctg tacgtccggt cagactggag gatgggcaat cggtattagc
     3421 cttacagctt tatgatgtga tcagcggtgg tgcaggtttt gcctccagcg caccggtaca
     3481 cattgaagct attttgcagg gaatggtgaa acagttaggc tgccgccatt gtgatactgc
     3541 atgtagtgaa tgcctgcttg attcccagac tcgccacgat catgatttgc tggatcgcaa
     3601 agcggcgctg gcctggcttg gcgatgactt cacgtattac atcggtttac cggatgaaga
     3661 gacattctcg ctgccggatg cccgctactg ccctggcgct attggcgata cgattcgccg
     3721 ggcgattaac gaaggcgctg aaaagctcac cctgtggatg actggcgcgc ccaatgagtg
     3781 ggatttatac gcccgtcagt tccgcgcggc tattcagagc tatcggctaa aagacaacgt
     3841 cgaggtcgat cttgttattc cggcgggagt ggatgatccg gacctgttgc atgaactatc
     3901 gcaatttact gcgctgggtg tgcgactgtg ccatgttgaa caggatctgc aattaccgat
     3961 tgttgcccag gttacctttg ccgatcgcgt gatgacgctg gcctctcgca gtcagcaggc
     4021 aacgattccg gggccggagt ggcacctgaa tgacgaactg gtagtgcgta gcctcggtta
     4081 taaaacggta gaactgagcg agtttattct gccagctaaa gccgccaacg ctgtcgagcg
     4141 ggtcaaagac attcagatcc acaaacagtt gaatggaccg ttgtcgcagt ttgggcaacg
     4201 cttctgggac gtgctgttta acgaccatga agaagcgcag tcactgatga aaaatacgcg
     4261 aattaccggc gttcactata ccgatcgcta tctgcaaaac ccggttgcgc tggcgttgtt
     4321 gggatcaatt ctgagaccgc taaaaacaaa gctgaccgat ggcgctgaag tgacgctgga
     4381 tacgctattt aaagataaag gtcgccccgg taaccggcct ttccatgact ggatgagcat
     4441 agcggatttt caggatttcg ctgatcagtg gtttgctgct gcgctgggca ggccggtaga
     4501 actgacggtg ttcgactcgc cgcgcgatat ccctcaccat cgtaaactga cggtgacttt
     4561 tgaggatggt caggtattga agatccgctt cgatcagggg atgggctact ggcgcatcaa
     4621 ctttgcatcg caatggcatt actttgattt ccgcgatgac gtttctttcc agttagtcaa
     4681 aatggctcag gcctgcaagg aagggaatgt cgccaacagc gaagagagtt gggcaacgga
     4741 tgtgctggtg gaggtgatcg cctcctgatg atgagccgct cccgatgtgg tgtcgggagc
     4801 ggtattttct ataacactta ccgcttattt gagatattca ccgaaaatgt cgagtaattc
     4861 ttgatgtata cacggccatt cctgacctaa attgacggta cacaagccaa tatcgaagcc
     4921 attaatttta taacgatgtt tcactgaggt gtctacgtgt gggtatatta ataacccccc
     4981 tgttttttcg ccattttcag gctttaacga ccataagtaa ctcatcagtt gatatagatt
     5041 ttgagaatga aacttttctg tacccattcg tcgtgaaaaa atgcttttat agtatttggc
     5101 atcaacgata agtattttcc ctgatgagtt aatggtgatg tcagtttcca ttcgaggtaa
     5161 caagctaagt gattgatccg atatgcttga tgcatcccat tttaaatgtg ggcgagttgt
     5221 atttacagat ggtaattcac gacggcaaaa ttcaaaaaga aaattttgat aaagtaatga
     5281 catctctttt tcatttcttt caaaatcata gaaacgataa tgtcctttgt tttgacctgg
     5341 aatagaatta ttggcgatga acttgcagac actgataacg aatttataat aacgcgtgtt
     5401 ctttccgcca ttcagatagc tgaaatgctg cggagttaaa tgaagagttc taatacccgg
     5461 taattttcta taaagtgaac gagcttcatc tctgatggtt gaatttaact tttcatgctt
     5521 aattaacatg gctaatgtgc tttttataat tcggttagcc agcgtatctt cattgagcat
     5581 atcaaacgta ctgacggttt tcccatgatt aagatggaag ccgcgtattg ttttagcaaa
     5641 ctctattcgc cctttgatgc caggaatgat ctcggtatta ggattgtaat caagctcaag
     5701 ccctcggcgt gaaagctgta aaaccccttt atttaataca taccccagga tatcaagaag
     5761 attgttaccg ggaatggctt caaggtcggc ctgcttaatt tcctgtaaat aaccccatgc
     5821 ataggtaagc atgtaataga tattacggac aggtatcacg ggctgttcca ctatgagtca
     5881 cctaataatt tgtcggccca tatctgttgt ttatagggat catcaaagaa atattcttcg
     5941 agtaaaggag cgatatccgt catcacaatt tccttaagcc attgcgtatc aggagatgtg
     6001 ccatcttcta acccggagca gaagtaacta tgcccaatgc ggaacccttt cccaaggata
     6061 gcggcctctt tgctgatttc ctggtttaac tcgttcattt tttggcataa agattcaaca
     6121 aatgaaggtt ctgctttttt attcagtaaa aaattacgga actgtggtgt atcaaaacct
     6181 ggctcaatat ctatgaaaga aaagcgtctg cgtagggcat aatcaacaac ggccagagag
     6241 cgatcggcag tattcattaa accgatgata taaacattct ccgggacata gaatcgttct
     6301 tcatcgtttt cggagtaggt taatggaaca gaccagtttt cacctcgttt atcatgttcc
     6361 attaacatca tcacttcgcc aaatacttta ctgagattgg cacgattgat ttcatctata
     6421 ataaaaacat actttttctc tggctgcttt atagcttgct gacaaaaatt gtaaaatata
     6481 ccgtctttac gtcggaagcc gacgccattt ggacgataac cttgtataaa atcctcatag
     6541 ctataagatt gatggaactg aaccatattg acgcgttgcg gagccttttc tcctgtcagc
     6601 aagtaagcca gacggcgtgc aacaaaggtt tttccaacgc cgggcggccc ctggaggata
     6661 atattttttt tgatggttaa tcgtttgagt atcgtctcta tcgtggtttc agggataaac
     6721 aaatcattta acgcatcttc cagacagtat gattcagttt ttgacatagg tggaataaca
     6781 ctcttgccag aattaaatat taatttatag tcgttgatta tgttgtccag catggaggca
     6841 aaccgggtat aatcaggacc ctgtgagact ttttgggaac aggcgtaata ggactgtccg
     6901 tattttttag gatatacacc tgaagtttcc tggaaatact ctgcgattgt tttaggtatg
     6961 tctgaagaga actgccattg gtcatgtagt ttattcgt
//
LOCUS       DAWDRC010000145         6976 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5126_length_6976_cov_2.241728, whole genome shotgun sequence.
ACCESSION   DAWDRC010000145 DAWDRC010000000
VERSION     DAWDRC010000145.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5126_length_6976_cov_2.241728"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1678 a         1676 c         1866 g         1756 t
ORIGIN      
        1 cgcgccagta tgctgatggt tgcagtggtg atgttctttg cctttagctg cctgtttacg
       61 ctgtctccgg cgaatatggc tgaagcgaaa gcgcagaata ttccagtgct ttcttatctg
      121 gctaaccact ttgcgtccat gaccggtacc aaaacaacgt ttgcgattac actggaatat
      181 gccgcttcca tcatcgcact cgtggctatt ttcaaatctt tcttcggtca ctatctgggg
      241 acgctggaag ggctgaatgg tttgattctg aagttcggtt ataaaggtga caaaaccaaa
      301 gtgtcgctgg gtaaactgaa tactatcagc atgattttca ttatgggctc cacctgggtt
      361 gttgcctacg ccaacccgaa catcctcgac ctgattgaag ccatgggcgc accgattatt
      421 gcatcactgc tgtgcctgtt gccgatgtat gccatccgta aagcgccgtc tctggcgaaa
      481 taccgtggtc gtctggataa cgtgtttgtt accgtgattg gtctgctgac catcctgaac
      541 atcgtataca aactgtttta atccgtaact caggatgaga aaagagatga atgaatttcc
      601 ggttgttttg gttattaact gtggttcgtc ttcgattaag ttttccgtac tcaatgccag
      661 cgactgtgaa gtattaatgt caggtattgc cgacggtatt aactcggaaa atgcattctt
      721 atccgtaaat gggggagagc cagcaccgct ggctcaccac agctacgaag gtgcattgaa
      781 ggcaattgca tttgaactgg aaaaacggaa tttaaatgac agtgtggtct taattggcca
      841 ccgcatcgct cacggcggca gtatttttac cgagtccgcc attattaccg atgaagtcat
      901 tgataatatc cgtcgcgttt ctccactggc acccctgcat aattacgcca atttaagtgg
      961 tattgaatcg gcgcagcaat tattcccggg cgtaactcag gtggcggtat ttgataccag
     1021 tttccaccag acgatggctc cggaagctta tttatacggc ctgccgtgga aatattatga
     1081 agagttaggt gtacgccgtt atggtttcca cggcacgtcg caccgctatg tttcccagcg
     1141 cgcgcattct ctgctgaatc tggcggaaga tgactccggc ctggttgtgg cgcatcttgg
     1201 caatggcgcg tcaatctgcg cggttcgcaa cggtcagagt gttgacactt ccatgggaat
     1261 gacgccgctg gaaggcttga tgatgggtac ccgcagtggt gatgtcgact tcggggcgat
     1321 gtcctgggtc gccagccaaa ccaaccagag cctgggggac ctggaacgcg tagtgaataa
     1381 agagtcggga ttattaggta tttccggcct ttcttcggat ttacgtgttc tggaaaaagc
     1441 ctgtcatgaa ggtcacgaac gcgcgcaact ggcaattaaa acctttgttc accgaattgc
     1501 ccgtcatatt gccggacacg ctgcttcatt acatcgcctg gatggaatta tattcaccgg
     1561 cggaatagga gagaattcaa gtttaattcg tcgtctggtc atggaacatt tagctgtatt
     1621 aggcgtagag attgatacag aaatgaataa tcgctctaac tcttttggag agcgaattgt
     1681 ttccagtgaa aatgcgcgtg tcatttgcgc cgtaattccg actaacgaag aaaaaatgat
     1741 tgctttggat gccattcatt taggcaaagt gaacgcgccc gcagaatttg cataatttag
     1801 ttgaagtatt gtagagagat tatttttcat gaaggtagat attgatacca gcgataagct
     1861 gtacgccgac gcatggcttg gctttaaagg tacggactgg aaaaacgaaa ttaatgtccg
     1921 cgattttatt caacataact atacacctta tgaaggcgat gaatcttttc tcgccgaagc
     1981 gacgcctgcc accacggaat tgtgggaaaa agtcatggaa ggcatccgta tcgaaaatgc
     2041 gactcacgat accaatattg ccaccacaat taccgctcat gatgcaggat atattgacca
     2101 gccgctggaa aaaatagtgg gtctgcaaac ggatgcgcca ttaaagcgtg cgctgcatcc
     2161 gttcggcggc attaatatga ttagaagttc attccacgcg tatggtcgtg aaatggatag
     2221 cgaatttgaa tacatcttta ccgatctgcg taaaacccat aatcagggcg tatttgatac
     2281 ttactcaccg gatatgctgc gctgccgtaa atctggtgta ctgaccggtt taccagatgg
     2341 ttatggtcgt gggcgtatca tcggtgacta ccgccgcgtg gcgctttacg gcatcagtta
     2401 tctggtacgt gaacgcgaac tgcagtttgc cgatctccag tcccgtctgg aaaaaggcga
     2461 agatctcgaa gctacgatcc gtctgcgtga ggagctggcg gaacatcgtc gtgcgctgtt
     2521 gcagattcag gaaatggcgg cgaaatatgg ctttgatatc tcccgtccgg cgcagaatgc
     2581 acaagaagcc gtgcagtggc tctacttcgc ttacctggcg gcagtgaaat cgcaaaacgg
     2641 cggcgcgatg tcgctggggc gcacggcatc gttcctcgat atctacattg agcgcgactt
     2701 taaagcaggc gtactcaatg agcagcaggc acaggaactg atcgaccact tcatcatgaa
     2761 gatccgtatg gtgcgcttct tgcgtacgcc ggaatttgat tcgctgttct ccggcgaccc
     2821 gatctgggcg acggaagtga tcggcgggat ggggttggac ggtcgaaccc tggtgaccaa
     2881 aaactccttc cgctatttac acaccctgca cactatgggg cctgcaccgg aacctaacct
     2941 gactattctg tggtcggaag ccttgccgat tgccttcaaa aaatatgccg cgcaggtgtc
     3001 tatcgtcaca tcctctttgc agtatgaaaa cgacgatctg atgcgtactg acttcaatag
     3061 cgacgattac gcgattgcct gttgcgtcag cccaatggtg attggtaagc agatgcagtt
     3121 cttcggtgcg cgcgctaacc tggcgaaaac gctactgtac gcaatcaacg gcggaatgga
     3181 cgagaagctg aagattcagg ttggcccgaa aacggctccg ttgatggatg acgtgctgga
     3241 ctacgacaaa gtgatggata gcctcgacca cttcatggac tggctggcgg tacagtacat
     3301 cagcgcgctg aatatcattc actacatgca cgacaagtac agctacgaag cttcgctgat
     3361 ggcgctacat gatcgtgatg tctatcgcac catggcgtgc ggcatcgcgg gcctgtcggt
     3421 ggcaacggac tccctgtctg ccatcaaata tgctcgtgtg aaaccaatcc gtgatgaaaa
     3481 cggcctggcg gtggatttcg agattgacgg cgaatatccg cagtatggca acaacgacga
     3541 gcgcgtagac agcattgcct gcgatctggt tgaacgcttt atgaagaaaa ttaaagcttt
     3601 gccaacctat cgcaatgctg tgccgaccca atcgattctg actatcacct ctaacgtggt
     3661 gtacggtcag aaaaccggga acacgccgga cggtcgccgc gccggaacac cgttcggacc
     3721 gggtgctaac ccgatgcacg gtcgtgatcg caaaggcgcc gtggcctcat tgacgtcggt
     3781 ggcgaaactg ccgttcacct acgccaaaga tgggatctcg tacaccttct ccatcgtccc
     3841 tgcggcgctg ggcaaagaag atccagtacg taaaaccaac cttgtcggcc tgctggatgg
     3901 gtatttccac cacgaagcgg atgtcgaagg cggtcaacac ctcaacgtca acgtaatgaa
     3961 tcgggaaatg ctgctggatg ccattgagca cccggaaaaa tatcctaacc tgacaatccg
     4021 tgtctctggc tacgccgtgc gcttcaacgc actgacccgt gaacagcaac aggatgttat
     4081 ttcacgtacc tttacccagg cgctctgacg ccggaggatg tatgaaaaag attatcgaaa
     4141 cgcaacgtgc cccaggcgca atcggccctt atgttcaggg cgttgattta ggcagcatgg
     4201 tcttcacctc cgggcaaatt ccggtttgcc cacagaccgg tgagatcccg gctgatgtgc
     4261 aagatcaggc gcgtttaagc ctcgaaaacg tcaaagcgat cgtggttgct gccgggctga
     4321 gtgtgggcga tatcatcaag atgaccgtgt tcatcaccga tctgaatgat tttgccacca
     4381 tcaatgaagt ctataagcag ttcttcgatg aacatcaggc gacctacccg acccggagct
     4441 gtgtgcaggt cgcgcgtttg ccgaaagatg tgaagctgga aattgaagcc atcgcagtac
     4501 gtagtgcgta aggcctcgtg agcgggacgg tcgctaggtc gttccgctcc acttcactga
     4561 acggcaatcc gagggtgtgg atatgattag tgcattcgat attttcaaaa ttgggattgg
     4621 tccctccagt tcgcatacag tggggccaat gaatgccgga aaaagtttta ttgatcggct
     4681 ggaaagtagc ggcttattaa ccgcgacgag ccatattgtg gtcgatctgt acgggtcgtt
     4741 gtcactgacg ggcaaaggcc atgccacgga tgtcgccatc atcatgggac tggcaggaaa
     4801 cagtccgcag gatgttgtca ttgatgagat ccctgcattt atagagttaa tgacgcgcag
     4861 cgggcggttg ccggtggctt ctggcgcgca tattgttgat tttcctgtag caaagaacat
     4921 tatcttccat cccgaaatgt tgcctcgcca tgagaacgga atgcggatca ctgcctggaa
     4981 gggacaggaa gagctattaa gcaaaaccta ttactctgtc ggcggcgggt ttattgtcga
     5041 agaagaacac ttcggcctgt cgcacgatgt cgaaacaccc gtaccttacg atttccactc
     5101 agcaggtgaa ctgctgaaaa tgtgtgatta caacggcctg tctatatctg gcctgatgat
     5161 gcacaacgag ctggcgctgc gcagtaaagc ggaaattgac gcagggtttg cccgtatctg
     5221 gcaagtgatg catgacggta ttgaacgtgg aatgaatact gaaggcgtgc tgcctggtcc
     5281 gctcaatgtg ccgcgccgtg ccgtagcgct gcgtcgtcaa ctggtttcca gcgataacat
     5341 ctctaacgat ccgatgaatg tcatcgactg gatcaacatg tacgcgctgg cggttagtga
     5401 agaaaacgca gctggcgggc gcgtggtaac ggcaccgact aacggtgcgt gcggcattat
     5461 tccggcagtg ctggcttatt acgataagtt ccgtcgtccg gtaaacgagc ggtcaattgc
     5521 ccgctatttt ctggcagcgg gggcaattgg cgcgctgtat aaaatgaacg cctccatctc
     5581 tggcgcggaa gtcggctgtc agggggagat tggcgtggcc tgttcaatgg cggcggcagg
     5641 gttaactgaa ctactgggcg gcagtccggc gcaggtatgc aatgcggcgg aaatcgcgat
     5701 ggagcataac cttgggctga cctgcgatcc ggttgccgga caggtacaaa tcccgtgcat
     5761 tgaacgtaat gccattaatg ccgtgaaagc agtaaacgcc gcgcggatgg cgatgcgccg
     5821 cacctcggca ccgcgtgttt cactcgataa agtgatcgag acgatgtatg aaaccggcaa
     5881 agatatgaac gataaatacc gcgaaacatc acgcggagga ctggctatta aagtggtctg
     5941 cggctgacat tgctgtcagc tttcatcctt gggtgcacaa atgtgcacct tttttttgtg
     6001 atctgccgca aatagagaat gaatattctc ttgcgattaa tttagtagaa aattcttatc
     6061 catctccgtt atttcaaagt cttgattgtg aagattttct ctgacctcgc gcgtatcatt
     6121 tctttattct aataactcgc ctgcaaaaac gtagaccgca tatcgccttg atgcggctcg
     6181 gaaaaaaata ataagcccta caaaatattg agcgagaaat tatggaaatt gcatcgaata
     6241 aaggcgtcat tgcagacgct tcgaccccgg cgggtcgtgc tggaatgagt gagagcgagt
     6301 ggcgagaagc gatcaaattc gacagtactg acaccggctg ggtgattatg agtatcggga
     6361 tggcgattgg cgcggggatt gtttttctcc cggtgcaggt cggtttgatg ggattgtggg
     6421 tatttttgct ctcatcggtg attggttacc cggcaatgta tctgtttcag cggttgttta
     6481 ttaatacgct ggcagaatca ccggagtgta aagattaccc gagcgtcatc agcggttatt
     6541 taggtaaaaa ctggggcatc ctgttaggtg cactctattt cgtaatgctg gtgatttgga
     6601 tgttcgtcta ttccaccgcc atcaccaacg atagtgcttc ctacctgcat accttcggcg
     6661 tgacggaagg gttgctgtga agtggtcaac aaaaactggc caccgagtta gagttttttc
     6721 cagtatcgat tttccgattc gtttggtggt aacccaccat tatattcgtg cggtcttagt
     6781 gcgctgtaat atccaacgat atagtccgtt attgcgtgag ctgcatcgct gaagcttaca
     6841 tagcccgtcg ccggcaccca ttcgttcttc agactcctga agaagcgctc cattgggctg
     6901 ttatcccagc agtttccacg ccgactcata ctctgcctga tccggtatct ccacagtaac
     6961 tgccggaact gcctgc
//
LOCUS       DAWDRC010000146         6962 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5137_length_6962_cov_2.725206, whole genome shotgun sequence.
ACCESSION   DAWDRC010000146 DAWDRC010000000
VERSION     DAWDRC010000146.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5137_length_6962_cov_2.725206"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    410..419
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3166..3175
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3659..3668
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2017 a         1537 c         1487 g         1891 t
ORIGIN      
        1 gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc atgccttcaa
       61 cgctaacatt ttagataaag attacgcctt ctctgacggc gagatcctga tggtagataa
      121 cgctgttcgt acgcattttg aaccttatga gcggcatttt aaagaaatcg gatttaatga
      181 aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg tgcctgttcc
      241 tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa tgattgctta
      301 tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt tttcttgcct
      361 gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtn nnnnnnnnnt
      421 aacggtgtgc tatccgtttc cgggaaagtg agaaatattg tcaacatgaa gctggcgcac
      481 ccatggaagc tgaaagatat ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa
      541 aaactaaagc aagagcaaac gacattctca cagattcttt tagatgccag aatgcagcac
      601 gcaaaaaatt tgatacgcgt agaaggttca gtcaataaaa ttgccgaaca atgtggttat
      661 gcgagtacat cttattttat ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga
      721 gtttctaagg agtaccgttg tcaaaggcac acgggtttga acacggataa cacgatgagt
      781 gctttagcta tttgattatt tgctaacgag tagtcaacca cgcacgttac gtagaaatta
      841 aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta ttgaaataaa
      901 taagtgacat tcatcacata tttatgcatt tgcataacct gttgcatgat tatttatgat
      961 ctcaattcgg cattttgtca gtaaaataca ataatttatt aaatatcaat agattagttg
     1021 tttatcggcg agaaattact tagtagaaca gaaagtaatg tcaacgcttt atggactgtt
     1081 atttctcttt ttttagctta atctgctatc tctttatgtg actaacttca cttacatcca
     1141 cttatttctc ttcgtaaaat tactttggaa ttaagtacaa taagaagagg aacatttatg
     1201 aagtctgcat taaagaaaag tgtcgtaagt acctcgatat ctttgatact ggcatctggt
     1261 atggctgcat ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac
     1321 gttgctttct cagactttac gccgactgaa tacagtacca agggaaagcc aaatattatc
     1381 gtactgacta tggatgatct tggttatgga caacttcctt ttgataaggg atcttttgac
     1441 ccaaaaacaa tggaaaatcg tgaagttgtc gatacctaca aaatagggat agataaagcc
     1501 attgaagctg cgcaaaaatc aacgccgacg ctcctgtcat taatggatga aggcgtacgt
     1561 tttactaacg gctatgtggc gcacggtgtt tccggcccct cccgcgccgc aataatgacc
     1621 ggtcgagcgc ccgcccgctt tggtgtctat tccaataccg atgctcagga tggtattccg
     1681 ctaacagaaa ctttcctgcc tgaattattc cagaatcatg gttattacac tgcagcagta
     1741 ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa acaaacgcgt
     1801 gactatcatg acaacttcac cacctattct gcggaagaat ggcaaccgca aaaccgtggc
     1861 tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc cccttcactg
     1921 ttcaaaaacc gtgaacgcgt ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag
     1981 gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc cttttatgct ttacctagct
     2041 tataatgctc cgcacctgcc aaatgataat cctgctccag agcaatatca gaagcaattt
     2101 aataccggta gtcaaacagc agataattac tacgcttccg tttattctgt tgatcagggt
     2161 gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac aattattctc
     2221 tttacctccg ataatggtgc agttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa
     2281 ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat gtggtggaaa
     2341 ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt ctacccgaca
     2401 gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg cgtttccttg
     2461 ctgccctggt tgcaagataa gaaacaaggc gagccacata aaaatctgac ctggataaca
     2521 tcttattctc actggtttga tgaggaaaat attccattct gggataatta ccacaaattt
     2581 gtccgccatc agtcagacga ttacccgcat aaccccaaca ccgaggactt aagccaattc
     2641 tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa taatcagtta
     2701 ggtctataca aactgacgga tctacagcaa aaagataacc ttgccgccgc caatccgcag
     2761 gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acaacagcca gccaccgctt
     2821 agcgaggtaa atcaggagaa gtttaacaat atcaagaaag cattaagcga agcgaaataa
     2881 ctaaaccttc atgcggcgga tttttccgcc gccttattga gcgagatagc gatgcacgtt
     2941 acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa
     3001 aaagagtcgc agtttactca tgaaaaatgg atggatgaca gcactctgaa agagttcatc
     3061 aaacaatata tcgcagcgtc tggcaatcag gtctatttta cctggcaagg cggtgaaccc
     3121 actctggctg gcctggattt tttccgtaaa gttattcact atcaannnnn nnnnnacgct
     3181 atgcaggcca aaaacgaatt tttaatgcat tacaaacgaa tggcatttta ttgaataatg
     3241 aatggtgtgc ctttctcaaa gaacatgagt ttctggttgg tatctcgatc gatggccccc
     3301 aagagctaca tgaccgctac agacgcagta attcaggtaa cggtactttt gcaaaagtga
     3361 tagcagccat cgagcgtctg aaatcatatc aaatagagtt taatacgtta accgtcatta
     3421 ataacattaa tgtccattac cctcttgagg tttatcattt tttaaaatct atcggcagta
     3481 aacatatgca atttatcgaa ttgctcgaaa ccgggacgcc gaatattgat ttcagtggtc
     3541 atagtgagaa cacattccgt atcattgatt tttctgtgcc tctcacggct tatggcaagt
     3601 ttatgtcaac catttttatg caatgggtta aaaacgatgt gggtgaaatt ttcatccgnn
     3661 nnnnnnnnga taatctggtt gttgaaagta atggagacat ttacgaatgc gaccattttg
     3721 tctatccaca gtacaaaatt ggaaacatta ataaatcgga actcaaaacg atgaacagtg
     3781 tacaactgac agcgcaaaaa aaacggattt cagcgaaatg tcagcaatgt gtatataaac
     3841 ctatctgcaa tggtggttgc cctaagcatc gtattactaa agtaaacaat gagactgttt
     3901 cctatttttg cgaaggttat aaaatccttt tttcaaccat ggtaccttat atgaacgcca
     3961 tggtggagtt agctaagaac agagtaccgc tttaccacat tatggatgtt gcaaaacaaa
     4021 tggagaataa ttaataacgc tcccccttag ctccattctt agcctccttc ttaagacctt
     4081 aacaaggcac ccgacataaa ttcctgtcgg gtgtttcgtc ttcatcatct tcctttgcga
     4141 aattattgcc gtatctaata aaggcgatgt ttccctgcgc agcaaaaatc acttatcgcg
     4201 ctggctaata aagcagtaag cggaagcggc gttggtgaac tctcctcttc cagcctgaaa
     4261 cgctggagtg cggaaaactc ggtaaccatg agcagtaaag tcagtggcat gaatacgttg
     4321 ctctccgtta gcgcgcggac taataaccct gaacctggtt tccagttgat taaccagcga
     4381 atcacctaca gcacgattaa cgacaatatt tgggcatcgc tacaaaatgc tcaaattcag
     4441 gcgttaaaaa cgctcgacca gcgtccagcg gagaaattcg cccagcagat gtatgagacg
     4501 cgctatgccg atgaccgcac gaaattaccg caagaaaatc agattgcaca gtttactgcc
     4561 gcagatgcgc tggctgccga tcgccagttg ttttcatctc cagcggatat cacgtttgtc
     4621 attgtcggta atgtcgcaga agacaaactc gtggcgttaa ttacgcgtta cttaggatca
     4681 atcaaacact ctgattcgcc attagccgca ggtaaaccat taactcgcgc gacggacaac
     4741 gcatcggtta ctgtaaaaga acaaaatgaa ccagtggcac aggtttcaca gtggaagcgt
     4801 tataattccc ggacacctgt taatctggcg acgcgtatgg cgcttgatgc ttttaacgtc
     4861 gcactggcaa aagatctacg cattaatatt cgtgaacagg catccggggc atacagcgtt
     4921 tcttctcgcc tctcggttga tcctcaggcc aaagatatca gtcatttgct ggcttttact
     4981 tgtcaaccag aacgacatga tgaactgtta acgttagcga atgaagtgat ggttaagcgt
     5041 ctggccaaag ggatcagtga gcaagaactg aacgaatacc agcaaaacgt tcagcgcagc
     5101 ctcgatatcc aacagcgtag cgttcaacaa ttagcgaaca ccattgtaaa tagtcttatt
     5161 caatatgacg agcctgcagc atggattgag caggagcaat tgttgaaaca aatgacggta
     5221 gagaatgtta acactgctgt taaacaatat ctttctcatc cggtgaatac ctataccgga
     5281 gtattattgc caaaataatc acagccccgt caacacatcg ttggcgggga ttttatcaat
     5341 attcgctatt tttatgtaat aattttataa atgtgttcaa agtaaataac cgtgtactaa
     5401 taatgatatt ataaggtctg atttttacgt gataattcag gagacgatag aaaacacata
     5461 aaataacagc ataaaacacc ttacctccac acagaattct ctattatgtt gttttttaat
     5521 aaaaatacat tattcacgca atatctcgcg ataaataaca ttaggatttt gttatttaaa
     5581 accgagtcct ttgcacttgc ttactttatc gataaatcct acttttttaa tgcgatccaa
     5641 tcattttaag gagtttaaaa tggataagaa gcaagtaacg gatttaaggt cggaactact
     5701 cgattcacgt tttggtgcga agtctatttc cactatcgca gaatcaaaac gttttccgtt
     5761 gcacgaaatg cgtgatgatg tcgcattcca gattatcaat gacgaattat atcttgatgg
     5821 caacgctcgt cagaacctgg ccactttctg ccagacctgg gacgacgaaa acgtccataa
     5881 attgatggat ttgtcgatca ataaaaactg gatcgacaaa gaagaatatc cgcaatccgc
     5941 tgcaatcgac ctgcgttgcg tcaacatggt tgccgatctg tggcatgcgc ctgcaccgaa
     6001 aaatggtcag gccgttggca ccaacaccat tggttcttcc gaggcctgta tgctcggcgg
     6061 gatggcgatg aaatggcgtt ggcgcaagcg tatggaagct gcaggtaagc cgactaacaa
     6121 accgaacctg gtgtgcggtc cggtacaaat ctgctggcat aaattcgccc gctactggga
     6181 tgtggagttg cgtgagatcc ctatgcgccc cggtcagttg tttatggacc cgaaacgcat
     6241 gattgaagcc tgcgacgaaa acaccatcgg cgtggtgccg actttcggcg tgacctacac
     6301 cggtaactat gagttcccgc agccgctgca cgatgcgctg gataaattcc aggccgacac
     6361 cggtatcgac atcgacatgc acatcgacgc cgccagcggt ggcttcctgg caccgttcgt
     6421 cgccccggat atcgtctggg acttccgcct gccgcgtgtg aaatcgatca gtgcttcagg
     6481 ccataaattc ggtctggctc cgctgggctg cggctgggtt atctggcgtg atgaagaagc
     6541 gctgccgcag gaactggtgt tcaacgttga ctacctcggc ggtcagattg gtactttcgc
     6601 catcaacttc tcccgcccgg cgggtcaggt gattgcacag tactatgaat tcctgcgcct
     6661 cggtcgtgaa ggctatacaa aagtacagaa cgcttcctac caggttgctg cttatctggc
     6721 ggatgaaatc gccaaactgg ggccgtatga gttcatctgt acgggtcgcc cggacgaagg
     6781 cgtcccggca gtttgcttca aactgaaaga tggtgaagat ccgggataca ccctgtatga
     6841 cctctctgaa cgtctccgtc tgcgcggctg gcaggttccg gccttcactc tcggcggtga
     6901 agccaccgac atcgtggtga tgcgcattat gtgtcgtcgc ggcttcgaaa tggactttgc
     6961 tg
//
LOCUS       DAWDRC010000147         6912 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5184_length_6912_cov_2.798308, whole genome shotgun sequence.
ACCESSION   DAWDRC010000147 DAWDRC010000000
VERSION     DAWDRC010000147.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5184_length_6912_cov_2.798308"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4423..4432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4960..4969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6456..6465
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1585 a         1714 c         1869 g         1714 t
ORIGIN      
        1 ttggcggtag tgaagcagtc ctggaacagg cgcgacgctt tcttgaaatc cgctggttaa
       61 gcgcaccggc gtcgctggcg aatctggtat tacttggttg gttactcggc gtgcaatatg
      121 cccgtgcgcc agtaattttg ttagtggtcg gcaatatcct caacattgtg ctggatgtct
      181 ggctggtgat ggggctgcat atgaacgtgc agggcgcggc tctggcaacg gttattgcgg
      241 aatatgcaac attgctgatt ggtctgctaa tggtgcgtaa aatcctcaaa ctacgcggaa
      301 tctccggcga aatgctgaaa acagcctggc gaggaaactt ccgtcacttg ctggcgctta
      361 accgcgatat catgctgcgc tcgctgttgc tgcaactctg tttcggcgcg gtcactgtac
      421 ttggcgcgcg actggggagt gacattatcg ctgttaacgc ggttctgatg acgctactca
      481 cctttaccgc ctatgcgctg gatggttttg cctacgcggt tgaagcacat tccggtcagg
      541 catacggtgc gcgcgacggt agtcaattgc tggatgtctg gcgggcagcg tgccgccagt
      601 cggggatcgt agcgttactg ttttcggtgg tttatttgct ggcaggggaa cacatcattg
      661 cgttgctgac gtcgttaacc cagattcagc agctggctga ccgctatctt atctggcagg
      721 tgattttgcc gttggtcggc gtctggtgtt atctgctgga cggcatgttt ataggcgcaa
      781 cgcgtgccgc cgaaatgcgt aacagtatgg cggtggccgc cgcaggtttt gcactgacgc
      841 tccttacgct gccgtggctg gggaatcatg gtttgtggct ggcgttaacc gtctttctgg
      901 cgttacgcgg gctttctctg gcggctatct ggcggcgtca ctggcgtaac gatacctggt
      961 ttgtcgcaac gtgacggtta aaaattctga ataaataatc ctaagccaaa ttgctgacta
     1021 cacttaatct cacgttcaga agaaaagtga acgtactctc attcacaacc taacgatgag
     1081 gtcttgatta tgaataaaga tgaagccggc ggtaactgga aacagtttaa aggtaaagtg
     1141 aaagagcaat ggggcaaact gaccgatgat gatatgacga tcattgaagg aaaacgtgat
     1201 cagttagtgg gtaaaatcca ggaacgctac ggttatcaga aagatcaggc agaaaaagag
     1261 gttgatagtt gggaaaaacg ccatgattac cgctggtaat taatccctcc tgcccgacat
     1321 gtacaaggat gtacaccctt ttagcgaggt ttctttttca cctgaataga gtggtcatga
     1381 tggcactgtt cgggatgacg acacgcttct acttctacac atgctgaaca taatccatgt
     1441 gcttcaataa cattatgccg cagggcaaaa cccatttttg ccgccagcgt atgcatgatg
     1501 tcttccacgc cttcagcaca ttcttctttc accgcgccgc agcgatcgca aataaacatg
     1561 gctgaagtat gggtgggctg atcgaacaga tgacagagca cataactgtt ggtggattcc
     1621 accttatgca caaaaccttg ttcaagcaga aactccagcg cgcgataaac cgttggcggc
     1681 ttggcttgcg gttcagcttc gcgcagcaaa tcaagcagat cataagcgct gatagcgcca
     1741 tcctgcagac tcatcaggcg caacacttcg agacgctgtg gggtcaggcg cacattacgc
     1801 tgtgcgcaga ttttttcagc ctgcgctaat aactcctgcg ttgtggtctt ttccattggg
     1861 cacctcgaca gtggggttaa gaatgccctt actttaccat agttctcgct taaacgccga
     1921 gaacagggtg tcatggctgt cacattgcct gatcgcatat tcgttatgct atagtagcgc
     1981 ccccaattta ccagatgctt aaaaagtcgc catgccagca gaatcacaca ctgtttatcc
     2041 cgcttaccgt ttctcgattg ccccgatgct tgactggacg gacagacatt gccgttattt
     2101 cctgcgtctg ctttcccgca atacgttgct gtataccgaa atggtgacca caggggcgat
     2161 tattcacggt aaaggtgatt acctggcgta cagtgaagaa gaacatccgg tagcgttgca
     2221 actcgggggt agcgatccgg cggcgctggc acagtgtgcg aagctggcag aagcgcgcgg
     2281 atatgatgag atcaacctga atgtcggctg cccgtctgac cgggtgcaga acggcatgtt
     2341 tggtgcgtgt ctgatgggta atgcgcagct ggttgccgac tgcgtgaaag cgatgcgcga
     2401 tgtggtgtcg attccggtga cggtgaaaac gcgtattggc atcgatgatc aggacagcta
     2461 tgaatttctc tgcgatttca tcaacaccgt ttccggcaaa ggtgagtgtg agatgttcat
     2521 catccacgca cgtaaagcct ggctttctgg gttaagtccg aaagaaaacc gtgagatccc
     2581 gccgctcgat tatccgcgtg tgtatcaact gaagcgtgac tttccgcatc tgacgatgtc
     2641 gattaacggt ggtatcaagt cgctggaaga agctaaagcg catttgcaac atatggatgg
     2701 cgtgatggtc gggcgcgagg cgtatcagaa tccgggtatt ctggcggcgg tagaccgaga
     2761 gatttttggt tcctcggata ctgatgctga tccggtggcg gtagtgcgcg ccatgtatcc
     2821 gtacattgag cgtgaactca gccagggtac gtatctcggc catattaccc ggcatatgct
     2881 gggcttgttc cagggtattc ctggcgcgcg gcagtggcgg cgttatttaa gtgaaaatgc
     2941 ccataaagcg ggtgcagaca ttaatgtgct ggaacacgcg ctcaaactgg tggcggataa
     3001 gcgttaactt ttcaccaaaa agtagtcaaa ttcaccacgc cctgcgcccc gtcgcggggc
     3061 gttttgctgt taaatcaata gattattttt ggcatgattc ttgtaatgcc agcaagagat
     3121 ttcatatttg ggagagcatc atgctggaac tactttttgt aattggcttt tttgtcatgc
     3181 tgatggtcac cggtgtttcg ttgctgggta ttatcgccgc gctgattgtg gcgacggcct
     3241 ttatgttcct cggcggtatg ctggcattga tgattaagtt gctgccgtgg ctgctgttgg
     3301 cggttgcggt ggtgtgggtg atcagggcga ttaaagcacc aaaagtgccg aaatatcagc
     3361 gttatgaccg ctggcgttac taagggattg tgcggatgat cacaacctaa ggttttatcc
     3421 ttagaacaaa ataggaattg ataatcaaat ctgtcactat tgcgcgtcaa acaaattcat
     3481 cgagctgtac cctacataca gccgaactat aaaagaaagg gcttcccagg tggaagccca
     3541 atttctttta tggaatcaac aggctggaac cctgagtcgc ccggctttcc agaatctcat
     3601 gcgcacgccg cgcatccttc agcggatatt tctgctgctc ggcgacatcg accttaatca
     3661 caccgctggc aatcaaagag aacagttcat tactggcctc ggttaattcc tcccgcgtgg
     3721 tgatatagcc ttgcagggaa gggcgtgtca catacaacga gcctttctga ttgagaatgc
     3781 ctaagttcac accggtaacc gcacctgatg agttgccaaa gctcaccatt aagccgcggc
     3841 gttgcaggca atccagcgac cgttcccagg tgtctctgcc cacggaatcg tacaccacgc
     3901 gcactttctt gccaccggtg atctctttta accgctcgac cagattctct tcacgatagt
     3961 taataacctg ccatgcgccc gcttttagcg cactctgcgc tttttgcgcg gttcccacgg
     4021 tgccgataag tttcgcgccc agggcttttg cccactggca ggcaatcaag ccaacgccgc
     4081 cagccgctgc gtggaacagg aactgctcat cgggtttaat ttcataggtt ttgcgcagca
     4141 gataataaac cgttaagcct ttcaggaagg atgccgcagc ttgctcaaaa gaaattgccg
     4201 caggcagaat cgccgcttta tccgcattaa tgttatgcac agagctgtaa gcgcctaatg
     4261 ccgattgtgc atagactacg cgatcgcctg ccttaatatg ctttacacca ctgccgactt
     4321 tactcacgat gcctgccgct tcggtgccta atccgctggg tagcgatggt ggcgggtaaa
     4381 ggccgctgcg gatatacgtg tcgataaaat tgatgccgat ggnnnnnnnn nnttctccgc
     4441 cggatcggca ggagtgaact ctacggcttg aagtacttcc gggccaccgt gcttgtgaaa
     4501 ttcaattcgt gttgccatgt gtcctccaga acgtatcgtc agggtctgct tcatatggta
     4561 aagtttcgac ccattcttta tctcggtaac tccattcact atggcaggaa ataaaccctt
     4621 caacaaacag caggctgaac cccgcgaacg cgatccacaa gttgccgggc taaaagtgcc
     4681 tccgcactcg atcgaagcgg agcagtcggt gttgggcggt ttaatgctgg ataacgaacg
     4741 ctgggatgat gtagccgagc gtgtggtggc agacgatttt tacacccgcc cacaccgtca
     4801 tatctttact gaaatggcgc gtttgcagga aagcggtagt cctatcgatc tgattaccct
     4861 tgcggaatcg ctggaacgcc aggggcaact cgatagcgtc ggtggttttg cttatctggc
     4921 agagctgtca aaaaatacgc caagtgcggc gaacatcagn nnnnnnnnnt atgcggatat
     4981 cgtgcgcgaa cgtgccgttg ttcgtgagat gatctccgtt gctaacgaga ttgccgaagc
     5041 tggttttgat ccacaagggc gtaccagtga agatctgctc gaccttgctg aatcccgcgt
     5101 ctttaaaatt gccgaaagtc gtgcaaacaa agacgaaggg ccgaagaaca tcgccgatgt
     5161 gctcgacgca accgtggcgc gtattgagca gttgtttcag cagccacacg atggcgttac
     5221 cggggtaaat accggttatg acgatctcaa caaaaaaacc gctggcttgc agccgtcgga
     5281 tttgatcatc gttgccgcgc gtccgtcgat gggtaaaacg acatttgcga tgaacctcgt
     5341 cgaaaacgcg gcgatgttgc aggataaacc ggtgcttatc ttctcgctgg agatgccatc
     5401 agaacagatc atgatgcgtt ctctggcgtc gctgtcgcgc gttgaccaga ctaaaatccg
     5461 taccggtcag ctcgatgatg aagactgggc acgcatttcc ggcaccatgg gtattttgct
     5521 cgaaaaacgt aatatctata tcgatgattc ctccggcttg acgccaacgg aagtgcgttc
     5581 ccgcgcacgc cgtattgccc gtgaacacgg cggcatcggg cttatcatga tcgactacct
     5641 gcaactgatg cgcgtaccgg cgctttccga taaccgtacg ctggaaattg cagaaatctc
     5701 ccgctcgctg aaagcactgg cgaaagaact gaacgtgccg gtggtggcgc tgtcccagtt
     5761 gaaccgttct ctggaacaac gtgccgacaa acgcccggtc aactccgacc tgcgtgaatc
     5821 tggctctatc gagcaggatg cggacttgat catgtttatc tatcgtgatg aggtgtatca
     5881 cgaaaacagt gatttaaaag gcatcgcgga aattattatc ggtaaacaac gtaacggccc
     5941 aatcgggacg gtacgcctga cctttaacgg tcaatggtcg cgcttcgaca actatgcggg
     6001 gccgcagtac gacgacgaat aataattatt ttatgaatta ggtaattaaa gcaaacactt
     6061 atcaaggaac acaaatgcaa gcggcaactg ttgtgattaa ccgccgcgct ctgcgacaca
     6121 acctgcaacg tcttcgtgaa ctggcgcctg ccagtaaaat ggttgcggtg gtgaaagcga
     6181 acgcttatgg tcacggtctt cttgagaccg cgcgaacgct ccccgatgct gacgcctttg
     6241 gcgtagcccg tctcgaagaa gctctgcgac tgcgtgcggg gggaatcacc aaacctgtac
     6301 ttttactcga aggctttttt gatgccagag atctaccgac gatttctgcg caacattttc
     6361 ataccgccgt gcataacgaa gaacagctgg ctgcgctgga agaggctagc ctggacgagc
     6421 cggttaccgt ctggatgaaa ctcgataccg gtatgnnnnn nnnnnccgtc tgggcgtaag
     6481 gccggaacag gctgaggcgt tttatcatcg tctgacccag tgcaaaaacg ttcgtcagcc
     6541 ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca aaatgcggcg caaccgagaa
     6601 gcaactcgct atctttaata ccttttgcga aggcaaacct ggtcaacgtt ccattgccgc
     6661 gtcgggtggc attttactct ggccacagtc gcattttgac tgggtgcgcc cgggcatcat
     6721 tctttacggc gtatctccgc ttgaagatcg ctccaccggt gccgattttg gctgtcagcc
     6781 agtgatgtcg ctaacctcca gcctgattgc cgtgcgtgag cataaagccg gagagcctgt
     6841 cggttatggt ggaacctggg taagcgaacg tgatactcgt cttggcgtag tcgcgatggg
     6901 ctatggggct gg
//
LOCUS       DAWDRC010000148         6889 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5198_length_6889_cov_2.424934, whole genome shotgun sequence.
ACCESSION   DAWDRC010000148 DAWDRC010000000
VERSION     DAWDRC010000148.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6889)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6889)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5198_length_6889_cov_2.424934"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3576..3585
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1898 a         1723 c         1532 g         1726 t
ORIGIN      
        1 ttcctgctgt ttcgccaaaa ggcggttgct gaatgatcca aatccactct acagaggcca
       61 gatataacaa gctgcccccc gttccagaag taaaaataag tccaagccag gttaacaagc
      121 tgaagtcagg tttttctccc gactgaccta acaagatatc gccataacgt gagaaggcca
      181 gccacagaca acaaagtaca accagaaaag tcatgaccag aaaaatcccg cccatttccc
      241 aggttaaaaa atgatgtatt ttatttatat accggctggt atcttgtgga tatatcgtca
      301 aacacagaat gattaccgca ataaataata ttgctgggat aaacacattc agttcaatca
      361 aagaaaagcg ttttttaaaa tagtgcattt cagtctcctg aatgttttca ttcaatgagg
      421 tcgaatattt cgccgttcct ttttcatgtg ttgcaattgt cgggaaaggc gtaaagaaaa
      481 aaaggcgcaa ataagtgaaa gagccgcaat acaatacaga gctaaaccac gctgatgctg
      541 ttgcagcatg aacaggaata tccccaaaaa gaggccgcag agaactgtgg ttatccagta
      601 attggcactt aagtgctcca ggtcatcatc cctcatggtc tttctcctgc aaggcgcaga
      661 caggctttca gctctctgcg ccttgttgtt atgccagacg tacgccctgc tgttgcagcg
      721 tgttttgcaa atcagggata gcaacctgac gcacggtgca attttgctgt agcgaaacca
      781 cggcggcagt acctgcggcc tcaccggtaa cggcacaaca catcatgtta cgcatagaag
      841 tatgtgcaac tacgcctgcg gaaatacagc gaccggcgac caaaaggttc tccacttttt
      901 gcggcaccag acaaccataa gggatctgaa agaaacgccc ggtcgttggc aaaatgagat
      961 aaccggaacc atcaataaat tccgggaaaa tccctacact gtccgaacaa cgcccctgat
     1021 taaggacgta atcatcagca atacgtattt ccccctcaat aagccgtgat tcacgcgtcc
     1081 ccagcgtggc accaaaattg cgtaaccgta cattttcaaa gcccggaaca tagtggcgta
     1141 gtgcgtcaat tgcccataat gcttgctgcc ttccggcaat ctcagctttg gttaaatcga
     1201 agacatcggt acagtcaaaa ccaaaggcgt acaccatatt catctggaaa gcctcaccgc
     1261 tttcagaaaa ggtcgaccag gttccggcaa tcgcctgggc gtcacctgga atcacaccat
     1321 cctgttgcgc gcgggtaaaa atatcctcca tatacgggct aaacatcggg tcttctttac
     1381 ccgtggtttg aatcgtccag tttttgcccc aatccgcgta ggtcggctta agttcttccg
     1441 caacaaaacg gttaaagcgt gccacatcaa cacctgcgca actgaacatc acggtgacgc
     1501 ccatcagttg gtccttgctc cgtttcgtcc agggcgcgcc tgcacgagcg gcaatatcag
     1561 catcccccgt gcaatcgacg attcttttcg ccagaattgc ccctcgacct gatttattct
     1621 cgacgataac accgcataac gtatccccgt ccttgatcac gtccacaacc caggagtgat
     1681 ataacggctc aacgccagat tccgtaatca tctgatcggc gacaacttta aatccttcag
     1741 tatctataac ttcgctaatt gactgaggtt ctggtcgtgt gaaacccagc gctcgtgcgc
     1801 ggccttcata ctcacgacag atcccttcac aatcttccgt ccccggatga cgataccagg
     1861 caaaactttc taccccgacc tgcgtcaata cgccgcctag cgacccgtag cgctcaacaa
     1921 tcatggttct ggcacctaac cgccctgccg caatggcagc ggataatcct gccggaccgc
     1981 ccccgataac cagaacgtcc gtttgtgcaa tgactggcgt ttgccgtgca ctttcattaa
     2041 tcatgtccac cattggttcc cccgtcagat tcaggatgcg caatccgcat tgttttgagt
     2101 gatttcattg acttgatttt tacttttacg attcacacgc tggaggacca gtgcggcgat
     2161 aaggccgatg aatgcgcaaa ccaacatgaa caggaaaaca tcctgataac cggcaatacc
     2221 cggtttgcgg tcgaccatat tgcccaccat cgtgtagcag tagatttccg ggaaataggt
     2281 cagtaaagag atcagacccg cagcagtccc tgtgactttt gccgggatcc gaacctcatc
     2341 aataattgag aagaacacgg cattcgcaga gtaaataaac acgcccaacg caaccatatt
     2401 cgccagcgca atagtgacgt agcgcgactc accgggcagg aagaaataaa cggtagtaaa
     2461 gacaatcatg ccgataaagg cgtaaatcat aaaacgcgtg cgtgacgcac agcggtcggc
     2521 cagttgtccg ccaattaatc cccccatcgc aaataacaca taggcgcgaa gaatactggc
     2581 aactgccact tccgattcgc ccatatgcag gatctgcgtg agatacggcg tgaccatgcc
     2641 aaagccgata taaattgacc acatacaaaa ggtcacaaca cctgccagcc aaaccgtcgg
     2701 cattttcagc acgaatgcca tatctgccag acggaaattc gctttatctt cgctgtgcgt
     2761 ttcatcctga caaacaaacc aggctaaaac accggccaga atagtcacta cggaataaaa
     2821 aataattgtc gcccgtaaac catcaacacc ttcgccaaat ttggcaaata ccggcacgga
     2881 aagaaatccc agtacaatgg aggttaatcc cttacccaga aaccagtaac cgtataagcg
     2941 tccttgttct tcgctggttc ccagggtacg aataattcgc acacacacgg cccaaaacgt
     3001 aaatacggtt gtaacagccc aaagtgcatg taacaaacag atcatagtat acgaggggaa
     3061 ggtcgcataa taaaaaccgc ttatcccggt ggaaattaat gaaaaggtca ttaatttacg
     3121 tgcagaaaat ctatcggcag cccaaccgcc aggtagatat aataaaaagt tcaccagacc
     3181 ataggctgtc attagaaaac caagttcagc attagtcgct cctgtagcct gctgcatcgc
     3241 gtcgtaatag gtttcccgta aataaggcaa tttatatatt gcacttccgg caataccaca
     3301 taacgccagc gtgatccaac gtttccagtt actggtcata tttattcccg aatgaagtat
     3361 ttaaaatgac accaacggcc tgctttgcac aggccgtatt attttttaaa gtcccaaatg
     3421 atgtgcagtt aaaattgcac aaagaacgtc ctgcgctgtt actacgaatg gcatattatg
     3481 gatagtttcc ccttcagcag tcgcggcttt agccactgcc cgtaatttat ctttattaat
     3541 ttccttcacg cctaacatat gaagatttgt cggcannnnn nnnnnaaatt gagtacggtt
     3601 tcaatttcct cctgtggtgc gttttccagt acgagctgta ccaggacgcc aaatgccact
     3661 ttttcaccgt ggtagcaatg atggcattcc tccagttgcg tcatgccgtt atgaatggca
     3721 tgagctgccg ccaggccgct actttcaaat ccaatgccgc taagataggt attagcttcg
     3781 ataatattct ctaccgcttt ggtgctaaca ccttgttcaa ccgcgacttt tgccttatag
     3841 ccatcttcca gtagcgtggt ataacagagt cgggcaatag ccagtgccgc cagtgtcgac
     3901 gccccacctg ccattgtggc ttttcctgac gcctgattgg ctctcgcctc aaaccaggtt
     3961 gacaatgcat ctcccatacc ggccaccagc agacgcactg gcgcggctga aatgacgcca
     4021 gtatccataa tcaccatatc cgggtttttc gggaaaaaca gatattcgct aaactgacct
     4081 tccggggtgt aaattactgc cagagaactg gttggcgcat ctgtggaagc aattgtcggc
     4141 accaccacca ccggaatttt ttggtaatac gctacggctt ttgccgtatc caacgtttta
     4201 ccgccgccaa taccaataac gacattatat ttacgctgat taaatttccc acgaattcga
     4261 gaaatttcat tttgcgagca ttcaccacaa aagatatcaa aatctgcagt aatattttct
     4321 tttgcaaagc tctggcttac tctgtcaccc accagaccgg tcacaaattc atctgcaata
     4381 attaatggtt ctgttcccaa actcgccgca tatgtagcaa tattatccag agaaccattt
     4441 ccctggatat atttagccgg acttataata atcttatcca tatcattcct cgcgtaactt
     4501 gttttgcttc actgttaacc tactcgttgc atccagtggc ggcaaacatt tcattcgcga
     4561 aaaattatgt tttatgatcg cgctgtaaaa aacatcatcc caaagcaata ataaaaatat
     4621 tttttatgct ttaatcacaa acccctaatc ttgtcaccga tcccatttca ttttggaaca
     4681 acacaatgaa ttaactgttt cattatgaat cttattatcg tgcgatttgt tttattgcgg
     4741 ctggagaatt aatctcgcaa gcatatttag cgcgatttac gtaatttatc agaacaggaa
     4801 tgactatgaa gaaattaatt aaccagattg attctgttgt cacagaacaa atggaaggac
     4861 tcatcgccac ctggccacac ctgcaggcaa attacgcgcc ccgatatgtg tggtgcaaac
     4921 aaaccgataa tgcagtggcg ctgatttcag gcggcggctc aggccatgaa ccactgcatg
     4981 caggttttgt cggtatggga atgttaaccg gtgcatgtcc tggtgaaatc ttcacatcgc
     5041 ccacaccaga tcaaatgatc gaatgtgcca aagcggttga taatggttct ggcgttctgt
     5101 ttttcatcaa aaattacact ggcgatattc tgaatttcga aacggcagtc gagatgttac
     5161 acgaagaagg catcgccgtt gggaccgtca ttattgatga tgatgtagcg gtaaaagata
     5221 gcctgtatac agccggacgt cgcggtgttg cgggtacagt ttttgttgaa aagattgtcg
     5281 gtgcggctgc acttcaggga tataacctgg ggcagtgcga gcaacttggc aaagatgtta
     5341 acaatgccac ccgttctttt ggcatcgcac tgtcagcctg tacggttccc gccgcgggaa
     5401 aaccctcatt tgaactggct gataatgaaa tcgaatttgg tgtcgggatc cacggtgaac
     5461 ccggcattga acgccgcact ttgcaagatc tgaacacttt aatcaacagc gtgatagcgc
     5521 aattactaga taataccccc tggcgacgca cattacgcca ttgggatcgc catgcggggg
     5581 gatggattga cgcatcatca atgaatgagt cattcgatca aaatgccgaa tatattgtgt
     5641 taattaacgg acttggcagc acgcctgaat cagagttata tggtgtagcc agggtattta
     5701 tgtgcgccgc acagcgccag ggaattaaaa ttagccgcca gttggtcggt aattattgta
     5761 catcattaga tatggccgga ttttccatta gcctgctgaa atgtacccca gagtttttgc
     5821 aactttggga tgccccggtc aatacgccag cgcttcgttg gggatgttaa taaggacatt
     5881 atatgaaaat tcaaaacaaa catgttattg cctggctgga gagttgtgct gcacatctta
     5941 ctgaacaaca agattttcta accgcacttg acagagatat tggtgatgcc gaccacggcc
     6001 tcaatatgaa tcgtggtttc agtgccgtga aagcgacgtt gcccgacatt gaacgtcagc
     6061 atattggcaa tatccttaaa aatactggca tgaaactact ttccagcgtt ggcggtgcca
     6121 gtggtcctct ttacggcaca ttatttattc gcgcttctgc ggcagtggga gacagaacag
     6181 aattaagtct ggaagagtgg ttggcgtgtc tggaagaagg gattgcaggc gttatcgcac
     6241 gaggaaaagc agaacaaggg gataaaacac tgtgtgatgt ctgggttcca gtcctccacg
     6301 aagccaaaaa gaacctgcaa gccggaatgt cgccttccct tctgctgaat acgatggtgc
     6361 aaaatgcggc aacagccgtc gataacacca tcaatatgca ggcgaaaaaa ggacgtgcca
     6421 gttatctcgg cgctcgcagt gtgggccatc aggatccagg cgcgacctcc tcctggctga
     6481 tgattaaggc tatgcaggaa ggattagcgg gataaattat ggttgccatt gttattgttt
     6541 ctcatagtct acgtctcgcc caggggattg aggaattagc actacagatg tctggagggg
     6601 acgttcctct ggccattgct gcaggtattg atgatccgca aaatccgatt ggcactgatg
     6661 ctattgccat tatgtctgct attgaatcgg tctggtcacc ggatggcgtt ctggtactca
     6721 tggatatggg cagtgcgctg cttagtaccg agatggcgct ggagctgctt tctgaagagc
     6781 aaagaagtgc aatttatctg ctcgccgccc ctgtagtgga aggtgccatg tcagcggtaa
     6841 cgtctgccgc agcggggctt tcggtgacgg agattatcgc tgaagtgga
//
LOCUS       DAWDRC010000149         6884 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5202_length_6884_cov_2.517060, whole genome shotgun sequence.
ACCESSION   DAWDRC010000149 DAWDRC010000000
VERSION     DAWDRC010000149.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5202_length_6884_cov_2.517060"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3201..3210
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5322..5331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1802 a         1797 c         1572 g         1693 t
ORIGIN      
        1 aagtacgtct gcggcaatgg aaaaggttgc acaggcaaaa tcaccgcatg ttgctgcgtt
       61 gggaagcgaa gctggcggca ctttgtacgg tttgcaggta ctggagcgta ttgaagcgaa
      121 tcagcgacaa aacttcaccc gatttgtggt gttggcacgt aaagccatta acgtttctga
      181 ccaggttccg gcgaaaacga cgttgttaat ggcgaccgga caacaagctg gtgcactggt
      241 tgaagcgttg ctggtactgc gcaatcacag tctgattatg acccgtctgg aatcacgtcc
      301 gattcacggt aatccgtggg aagagatgtt ttatctggat attcaggcca atcttgaatc
      361 agcggaaatg caaaaagcat tgaaagagtt aggggaaatc acccgttcga tgaaggtatt
      421 gggctgttac ccaagtgaga acgtagtgcc tgttgatcca acctgatgaa aaagtgccgg
      481 atgatgtgaa tcatccggca ctggattatt actggcgatt gtcattcgcc tgacgcaata
      541 acacgcggct ttcactctga aaacgctgtg cgtaatcgcc gaaccagtgc tccaccttgc
      601 ggaaactgtc aataaacgcc tgcttatcgc cctgctccag cagctcaatc gcctcgccga
      661 aacgcttata gtaacgtttg attaacgcca gattacgctc tgacgacata ataatgtcgg
      721 cataaagctg cggatcctga gcaaacagtc gcccgaccat cgccagctca aggcggtaaa
      781 tcggcgaaga gagcgccagt agttgctcaa gctgaacatt ttcttctgcc aggtgcagcc
      841 cataagcaaa agtagcaaag tggcgcagag cctgaataaa cgccatattc tgatcgtgct
      901 cgacagcgct aatacgatgc aaccgagcgc cccagacctg aatttgctcc agaaaccatt
      961 ggtatgcttc cggcttacgt ccatcacacc agaccacaac ttgctttgcc aggctaccgc
     1021 tgtccgggcc gaacatcggg tgtaacccca gcaccgggcc atcgtgcgcc gccagcatgg
     1081 cctgtaatgg tccatttttc accgatgcca gatcaaccag aatacaatct ttcggtaaag
     1141 gcggtaattt gccgataact tgctcagtaa cgtggatggg cacactaaca atcaccattc
     1201 cggcatcgga aacaatatca gccgctcgat cccagtcatg ttgctccaga atccgcacct
     1261 gataacccga gagtgtcagc atcttctcga acagacgtcc catctgaccg ccaccaccga
     1321 cgataaccac cgggcgcagt gacggacaaa gcgttttaaa tcctttgtcg ttttcactgg
     1381 agtaagattc acgcatcacc cgacgcaaaa catcctcaat cagatctggc ggtacaccca
     1441 gagcttccgc ctctgcacga cgcgaggcca acatagatgc ctcgcgctcc ggaacataaa
     1501 taggcagtcc aaagcggctt ttcacctcgc ccacttcagc aaccaattcc agacgcttcg
     1561 ctaataaatt cagcagcgct ttatcgactt catcaatttg atcgcgtaat gcggtcaatt
     1621 cagcaaccat aataaacctc ttaagccacg cgagccgtca gctgcccgtt cagatcctga
     1681 tgaatttcac gcagcaaggc atcggtcatt tcccagctaa tgcaggcatc ggttacggat
     1741 acaccgtatt tcatttcact gcgcggttgc tcggaagact gattgccctc gtggatatta
     1801 ctttcgatca tcagaccaat aattgagcga ttgccatctt tgatttgagc aaccacggat
     1861 tctgccaccg caggctgacg gcgataatct ttattggaat taccgtggct gcaatctacc
     1921 atcagagacg ggcgcagtcc cgcctgttcc atctcttttt cacattgcgc aacatccgca
     1981 gggctatagt ttggcgcttt accaccgcgc aggatcacat gcccgtccgg attcccctga
     2041 gtttgtagca acgcaacctg ccctgcctgg ttaatgccaa caaaactggg cggcggcacg
     2101 catagcgtta attgccgttg ccagactgcc atcggtgccg tttttaaaac caaccggcat
     2161 ggaaagcccg gaggccattt cacggtgagt ttgcgattcc gttgtacgag caccaattgc
     2221 tgaccagcta aacagatcgc ccaggtattg cgggctattc ggatctaacg cttccgtcgc
     2281 cagtggcagt cccatattca ccagctcaag cagcaattta cgcgcgatct gcagcccggc
     2341 ttctacatca aaagagccat ccatatgggg atcgttaatt aaccctttcc agccgacagt
     2401 ggtacggggt ttttcaaaat agacgcgcat taccagatag aggctatcgc tgacctctgc
     2461 ggcaagggct ttaaatcgac gagcatattc cagagcagtt tccggatcat gaatggaaca
     2521 aggaccacat actaccagca gacgaggatc gcgcccggcg ataatatctg aaatggtttt
     2581 acgcgagtca gcaatctggg cttcttgttg caggcttaat ggaaaagcgg ccttcagttg
     2641 ttccggagtc attaaaacct gttcgtcggt aatatgtacg ttattcagcg cgtctttctg
     2701 catgatggcg atcctgttta tgctcgtttg cgatagttga tcctcagcga ggatgacgta
     2761 acgataacac ataaagtaaa gttttcaatc catatttcgt acatatttct ttacataagc
     2821 aatttattcg cgctttttcc ccttatttct gtataaataa atttacacca gctttaaaaa
     2881 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaaatttatc gcccccttgt
     2941 tggctttact ggttagcgga tgtcagattg atccttatac tcacgcgcca accttgacca
     3001 gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc attaaagatg
     3061 acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
     3121 ggcaaaagaa aacttgccag accgatttta cttatgcccg gggactttct ggtaaaagct
     3181 ttcctgccag ctgcaataat nnnnnnnnnn taccagccaa ctgcatgaag tatggcgaaa
     3241 aggggctgat gaaaatgcca gcacaatacg attaaattaa ttacccccca aatcaattaa
     3301 gcatgagatt ttactcatta tgttttattt ctcacattga tgacgaccgc atgaaaggat
     3361 gatattttaa tgagcattct gaaaaaatat cgatgcattt cgagcgaaga tggtgaggat
     3421 ccctgaatgc gtttttctca ccgactgttc ttactgctga ttctcctgct gacgggagca
     3481 ccaatactcg ctcaggaacc ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt
     3541 attgtcagtt acacccgctg gcctgggcta tcagggccac caaagttatg tatcttttca
     3601 tcttcacgtt ttagtaccgc cttacaagaa aatgctgcca catctttacc ttatctgcct
     3661 gtcatcattc atactcaaca agaggcgata atttcaggct gcaatggttt ttattttggc
     3721 aacgagtcac cgacattcca gatggaatta accgaacaat atccgtcaaa ggcgttatta
     3781 ttaattgccg aacaaaatac cgagtgcatt attggtagcg ccttttgtct gatcatccac
     3841 aataatgacg tcagatttgc cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc
     3901 aacccggatg tcttaatgct cgcacggaag aaaaatgatg gataacgata attctcttca
     3961 taagcgcccc acgtttaaaa gagcattacg caacatcagc atgaccagca tatttatcac
     4021 tatgacgctg atctggctgc tgctttccgt gacgtcagtg ctaaccctga aacaatacgc
     4081 gcaaaaaaac ctggcactga cagcagcaac aatgacttac agtctggaag cagctgtcgt
     4141 ttttgccgat ggccctgcag caactgaaac actggcagcg ctgggccagc aaggccaatt
     4201 ttcaactgca gaagtgcgtg ataagcagca aaatattctg gcatcctggc attacaccca
     4261 taaggaacca ggcgatactt tcagcaattt cataagccac tggctcttcc ctgcccccat
     4321 cattcagccg attcgccaca atggtgaaac cattggcgaa gtacgcttaa ctgctcgcga
     4381 cagttcaatc agccatttta tctggttttc gctcgctgtc ctgaccggtt gtattctgct
     4441 ggcatcaggc atcgcaatta ccctcacccg ccatttgcac aatggcctgg tggaagcgct
     4501 gaaaaatatc accgatgtcg tacatgatgt gcgttctaac cgcaattttt cccgacgagt
     4561 ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc gacttcaaca gtctgctgga
     4621 tgaaatggaa gagtggcagc ttcgtttaca ggccaaaaat gcgcagcttc tacgtaccgc
     4681 gctacatgac ccattaaccg ggctggctaa ccgcgcagcg tttcgtagcg gcatcaacac
     4741 gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg atggcgataa
     4801 tttcaaatat atcaatgcta cctggggtca tgcgacgggc gatagagtct tgattgaaat
     4861 agcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgcc tgggcggcga
     4921 tgaattcgct atggtgctct atgatgtaca gtcggaatct gaagtgcagc agatatgctc
     4981 agcactgaca caaattttta atctcccgtt tgatcttcat aatggccatc agactaccat
     5041 gacattaagc attggttacg cgatgactat tgagcacgcc tctgcggaaa atttacaaga
     5101 gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc tggtgagata
     5161 acaaggatat atcgatatga taaagcacct gctagcaccc ctgattttca cctcgctaat
     5221 acttactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg ccgctatgca
     5281 atcttatgga tttactgaat ccgccggcga ctggtcgctg gnnnnnnnnn nggcttatca
     5341 gatgccattc tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacaaatc
     5401 caaaccatgg cagccaaatt ggcctcgaca gggctaacac atgcccgtat ggatggacac
     5461 accgacaact atggtgaaga cagttacaac gaagtcttat cattaaaacg ggcgaatgtc
     5521 gtggccgatg catgggctat gggtggacaa attccgcgca gcaatctcac cacacagggt
     5581 ttaggaaaaa aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaaccgc
     5641 cgcgtcgcag tggtgattac tgccccttaa aacacgagca aacagacaaa aaaagggcca
     5701 gccaattggc cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaaggcgct
     5761 ctttgatacg agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa
     5821 cagcaccacg acgtttgaca gaaatgctgt caactaccgg agagtgagtc tggaagacac
     5881 gctcaacgcc ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt
     5941 tacgaatagc gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa
     6001 cccatacttt cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct
     6061 gctcttgttc aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac
     6121 tgatatctcg ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt
     6181 tttgaactcc gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc
     6241 aggtcttcta agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc
     6301 atggttgccc gacagtaaca ctggcggaac ttccatccct tctaacactt caggccgcgt
     6361 atagtgcggg caatccagca atccttcagc aaaggaatct tccgttgctg aggcttcatg
     6421 tcccagtact cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc
     6481 accaccactg agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac
     6541 gcgctcatct ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccag
     6601 ttcgctgacg cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc
     6661 gccttcaccc gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat
     6721 taacatcccc ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt
     6781 gaagtcgcga ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt
     6841 taccccgtaa tcggtaattg cgcggaacat ttcaggaaac aggc
//
LOCUS       DAWDRC010000150         6857 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5219_length_6857_cov_2.726551, whole genome shotgun sequence.
ACCESSION   DAWDRC010000150 DAWDRC010000000
VERSION     DAWDRC010000150.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5219_length_6857_cov_2.726551"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2463..2472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6344..6353
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1504 a         1845 c         1806 g         1682 t
ORIGIN      
        1 cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg ctgcgcggaa
       61 ggcgagcgat gcttcctgcc attctccgtg tttcatcagc gactgaccca gtgtgctcca
      121 caacagcggg cgatcgccca cgtttttgat ctgttggcgc agcacttttt ccagctgttc
      181 cgggttattg gttttcagac gcgggatcgg cagcagcagg cgatcgtcat actggcgttt
      241 caggccgtcg atgataattt gctgggcagt atcatgatcg tcacactcaa taagatgttc
      301 cgccattgcc acctgcaagg ccacctgatg acgcgttttc cggctttggt ttttccacca
      361 gttacgcaaa ccttcactac cgttatcggc atgcgcctga tccatcaggc caatccatgc
      421 ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg ccattgatgg
      481 gatgatatcc agcagcgaac tccatgcacc agtgcggata tacgcctgtt ccgccagacg
      541 taatacttcc ggatggcgtg gcgtaacttc cagcagctta tccacgccgt gacgtgctgc
      601 atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg gaatggtgtc
      661 attgccggcc agctccgctg cgcgttccag atgttggttg gcgcgtgctt catcaccacg
      721 ttgttgcgcg gcttcggctg ccagtagata gttcaccacc ggttgttcag cgtgatcggc
      781 atttttcgcc atcagctttt caacttgctg ataatcgcct tccgccagtt tcagcagcgc
      841 ctgttcggtc tgcttacgtg cacggcgacg cttacgtccg acaaaccacc cacgggtgtg
      901 cgcgcctgtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca ctaccatcgc
      961 cagaatcaaa ataatcgcga ggcccgtgac gctagtttca atattgtagt tgtcggtctg
     1021 gatcagcacg taaccctgat ggccggcaat catcgggcca accacgatcc ccgcaatcag
     1081 caacacaaag agcaataaca cttttagcat ggttattctc cttgcggcgc ggctgccgga
     1141 gcatcagctt gcggtgcagg tgcaggtgca ggtgcaggtt tagcttccgt tgtccctgct
     1201 gccgattgtg ccagcaggtt acgcacgcgg gtctgcatca atttttccag catcgcctgg
     1261 ctttgcaggg tttccggaag atccatcgag atattttgct ggcttaactg gtccacctcg
     1321 tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt aagcacgtac ccaggtggag
     1381 acgttctcca gcgcctggcg ataagtctct tcctggtgac gcggtacagc ttgtgctgcg
     1441 accagcaggc gagagcgaat attttcgcgc agatagatat cctgatttgg cgctaacagc
     1501 ggtacggcag tgtcatcacg acggcgaatc gtaatgaagt tgtccataaa gttctgccag
     1561 cttttttgca gattgatacg ccattcgctg atggaactgg aaagctcttc accgtcagaa
     1621 tccatcggcg aaccatcgct gtcattatcg gccagacgca gattatctac ctgatttgaa
     1681 agctgattaa gcttaaggat gataccgtca taatccacct gcgataccgc agaaaggctg
     1741 gcgatatcat cggtaattgc ccggcgaacg gtaatcagac tcgggtcatt catatccgcc
     1801 aggctggcgt ctgcactttt cagcaacgct gcagcggtcg tgacgtcctg atcgctccac
     1861 agcttccgtc cggcgagttt caccagaaaa tcggcctggg ccagcagcca ggttttagca
     1921 tcgctgccgg aaatggtggc gaccttttgt tggacttcat ccagctgttt tgccagcgtt
     1981 tcttgctgac gattcgcctg ctcaagctgt acagcttgtt gcttgataat gccttccagc
     2041 tcggcttttt ggctctcctg ggctttttgc agtgccgtaa gttggttagc cagggcatcg
     2101 ctggtggcag tctgattgac ggcctgttgt ttaccccagc catacaaacc gacgcccgcc
     2161 gccagagcaa tagcgatggc caccgcgctg agaatcaatg cggtattgtt cttactcttt
     2221 ttttctgttg cgacaggttg tgacgtggtg tccacggcct ccctggtctc ttcaaccacg
     2281 gcggaggttt tttcttgttc cgtcattatg gcttcctgtt atgagagtta ttgtaatgcc
     2341 cgtaaaagcg catcgttgtc agcgttatcg gcgaccttaa tgtcttgcca gcccagttcc
     2401 cgggcgagtt tcgccaaacg ctcactgacg actaatagcc gacagcgtag taaccagtgc
     2461 tcnnnnnnnn nncacgatac cattgtggga tcagcgacca gagttgctgc aacatttcac
     2521 cgctggtaac aacgaccgtc gtcaccttgc gggattgcca gcgcatcgct tcttctgcac
     2581 catcgtaatg gattgcgcat cgttgataac attcacaaaa agtgacctca gcaccgcgcg
     2641 ccgtcaggga atccccaatt agctcacgac cgccattgcc acgtaatatc agcgcacgtt
     2701 tgcccgcaat attttgtaat tcaggtaatt gtagcaagac ttcgctgatt tcccgatcct
     2761 gcgggtagag aatcttctgt ccgcttacgg tatgtagtgc cagcgcggtg gtgcgtccaa
     2821 tggcgaaata atcaggtagt cggggccatt tacgatcttg ctgatgcagc tgtgattggg
     2881 caaaagcaac cgcgtgttgc gagagggcaa acaacagatc gctctctccc agcgctgcca
     2941 gttgatcagc aagttgcggt aattgtcgac ccggagaaaa ctcaatcagc ggaaaatgcc
     3001 aggccacctg ccccagtgtg cgcagacggc tcactaactc ttctcctgcg ggagacgggc
     3061 gggtgaccag gatactcatg ccggggcgtc tccgttatag acttcagcga ggatctcgcg
     3121 cgcgccgtta ttcagtagct cttctgccag cgaaatcccc atttgttcgg catcttgcgg
     3181 cgcaccgcgg cgttcaccgc gaataatctg cgaaccgtcc ggcgcgccga ccaacgcacg
     3241 cagccagatt tcgccatcaa taagctcggc gtagctacca attggcacct gacatccgcc
     3301 ttcgagacgg gtattcatgg cgcgttcggc ggtaacgcgc agtgcagttt cgtggtgatt
     3361 cagcgcggcg agcagctcgc gagtgcgtgc atcatcaagg cggcattcaa tgcccaccgc
     3421 accttgccct accgccggaa gagaaatctc gggtggcaac gcggcgcgaa tgcgggactc
     3481 cagacctaaa cgttttagtc cggctacggc aagaatgatg gcatcatatt cgccgttatc
     3541 cagtttgctc agacgagtgc cgacgttgcc gcgcagggag cggataatca gatccgggcg
     3601 gcgttcagcc agttggcact ggcgacgtaa actggacgtc ccgacgatac tgcctgccgg
     3661 tagcgcatcc agactgtcat aggtgttgga tacaaaggca tcgcggggat cttcgcgctc
     3721 gcaaatggtg accagcccca gaccttgcgg gaattcaacg ggcacatctt tcattgaatg
     3781 aacggcaata tcggcgcgat tttccaggag cgcgacttcc agctctttca caaataagcc
     3841 tttcccgcct actttcgcca gcggcgtatc aagaatcaca tcgccgcgcg tcaccatcgg
     3901 taccagttca acgaccaggc ccggatggct cgccatcaac ttgtctttga catagtgtgc
     3961 ctgccagagt gcaagtgggc tttggcgtgt ggcaattctt aaaacattgt ctaacatgct
     4021 tgttaccgtc attatcatcc gtggtccatc ctaacatcct tgccagagcg atgtcagtgt
     4081 tgtggtgaaa cgtagacgcc tgcgcaaacc gtaaaatgag gtctggcagt ggatcctgac
     4141 aggcgctttg agccttcgta ataaggaatt tacagagaat aaacggtgct acacttgtat
     4201 gtagcgcatc tttctttacg gtcaatcagc aaggtgttaa attgatcacg ttttagacca
     4261 ttttttcgtc gtgaaactaa aaaaaccagg cgcgaaaagt ggtaacggtt acctttgaca
     4321 tacgaaatat cccgaatgcc gcgtgttacc gttgattttg gcggaatcac agtcatgacg
     4381 ggtagcaaat caggcgatac gtcttgtacc tctatattga gactctgaaa cagagactgg
     4441 atgccataaa tcaattgcgt gtggatcgcg cgcttgctgc tatggggcct gcattccaac
     4501 aggtctacag tctactgccg acattgttgc actatcacca tccgctaatg ccgggttacc
     4561 ttgatggtaa cgttcccaaa ggcatttgcc tttacacgcc tgatgaaact caacgccact
     4621 acctgaacga gcttgaactg tatcgtggaa tgtcagtaca ggacccaccg aaaggtgagc
     4681 ttccaattac tggtgtatac accatgggca gcacctcgtc cgtagggcaa agttgttcct
     4741 ctgacctgga tatctgggtc tgtcatcaat cctggctcga tagcgaagag cgccagttgc
     4801 tacaacgtaa atgtagtctg ctggaaagct gggccgcctc gctgggtgtg gaagtcagct
     4861 tcttcctgat tgatgaaaac cgcttccgtc ataatgaaag cggcagcctg gggggcgaag
     4921 attgtggctc cacccagcat atactgctgc ttgacgaatt ttatcgtacc gccgtgcgtc
     4981 tcgccggtaa gcgtattctg tggaatatgg tgccgtgcga cgaagaagag cattacgacg
     5041 actatgtgat gacgctttac gcgcagggcg tgttgacgcc aaatgaatgg ctggatcttg
     5101 gtggcttaag ctcgctttct gctgaagagt actttggtgc cagcctttgg cagctctaca
     5161 agagtatcga ttccccatac aaagcagtgc tgaaaacact gctgctggaa gcctactcct
     5221 gggaataccc gaacccacgt ctgctggcga aagatatcaa acagcgtttg cacgacggcg
     5281 agattgtatc gtttggcctc gatccatact gcatgatgct ggagcgcgtc actgaatatc
     5341 tgacggctat tgaagatttc acccgtctgg atttagttcg ccgctgcttc tatttaaaag
     5401 tgtgtgaaaa acttagccgt gaacgcgcct gcgtaggctg gcgtcgtgaa gtgttaagcc
     5461 agttggtgaa agagtgggaa tgggacgatg ctcgtctggc aatgctcgat aaccgcgcta
     5521 actggaagat agatcaggtg cgtgaggcgc acaacgagct gctcgacgca atgatgcaga
     5581 gctaccgtaa tctgatccgc tttgcgcgtc gcaataacct tagcgtctcc gccagcccgc
     5641 aggatatcgg cgtgctgacg cgtaaactgt acgccgcgtt tgaagcatta ccgggtaaag
     5701 tgacgctggt aaacccgcag atttcgcccg atctctcgga accgaatctg acctttattt
     5761 atgtaccgcc gggccgcgcc aaccgttcag gttggtatct gtataaccgc gcgccaaata
     5821 ttgagtcaat catcagccat cagccgctgg aatataaccg ttacctgaat aaactggtgg
     5881 cgtgggcatg gtttaacggc ctgctgacct cgcgcacccg tttgtacatt aaaggcaacg
     5941 gcgttgtcga cttgccgaag ttgcaggaga tggtcgcaga cgtgtcgcac catttcccgc
     6001 tgcgcttacc tgcaccgaca ccgaaggcgc tctacagccc gtgtgagatc cgccatctgg
     6061 cgattatcgt taacctggaa tatgacccga cggcagcctt ccgcaatcag gtggtgcatt
     6121 ttgatttccg taagctggat gtcttcagct ttggcgagaa tcaaaattgc ctggtgggta
     6181 gcgttgacct gctgtatcgc aactcgtgga acgaagtgcg tacgctgcac ttcaacggtg
     6241 agcagtcgat gattgaagcc ctgaaaacca ttctcggcaa aatgcatcag gacgccgcac
     6301 cgccagatag cgtggaagtc ttctgttata gccagcatct gcgnnnnnnn nnntgaactg
     6361 cgtctttcca gcacccgcca ggaaaccggg cgcttcaagg cgctgcgcgt ctccggtcaa
     6421 acctggggct tgttcttcga acggctgaat gtctcggtac agaaactgga aaacgccatc
     6481 gagttttatg gcgctatttc gcataacaaa ctgcacggcc tgtcagtgca ggttgaaacc
     6541 aatcacgtca aattaccggc ggtggtggac ggctttgcca gcgaagggat catccagttt
     6601 ttcttcgaag aaacgcaaga cgagaatggc tttaatatct acattctcga cgaaagcaac
     6661 cgggttgagg tgtatcacca ctgcgaaggc agcaaagagg aattggtgcg tgacgtcagt
     6721 cgcttctact cgtcttcgca cgaccgtttc acctacggct caagctttat caactttaac
     6781 ttgccgcagt tctatcagat tgtgaaggtt gatggtcgtg aacaggtgat tccgtttcgc
     6841 accaaatcta tcggtaa
//
LOCUS       DAWDRC010000151         6853 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5223_length_6853_cov_2.392910, whole genome shotgun sequence.
ACCESSION   DAWDRC010000151 DAWDRC010000000
VERSION     DAWDRC010000151.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5223_length_6853_cov_2.392910"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1621 a         1614 c         1846 g         1772 t
ORIGIN      
        1 gttacggtca gtagtttcaa aacaggtcat cgcgcgcagg ccaactttta aaaaggcgtc
       61 gcgcaatggg gagagcgacc cgccgatata ggctggagag gcatggtgat cgataaccga
      121 tgtacatccg ctcttaattg cttccaggga acaaatcagt ccgctgtaat agagcgactc
      181 ttcatcaagg gcgcgatcga gccgccacca gagatttttc agcgttgaga tgaaatccgg
      241 gcagggggcg atgtttgcca taattccgcg ggaaagcccc gagtaaaaat ggttgtgcga
      301 gcagacaatt cccggcatca caatccgacc atgcatctct ttgtagctgg catcgggata
      361 gcgttgcgtc agggcatcgt cgatagcgac aatcacatca ttttcgatgg cgatatcaac
      421 gctttcctgc actttcgccg ggtgtaactg cactgcggtg acattcttca gaatcaacat
      481 gattacacct ccacgcggcc cagcagataa tgatgatgct ggtggacatg gctaatgatg
      541 cggcacatat cgttcagctc cggtggtacg ttgttgaact gaccttcgct gtcgatgttt
      601 aacacccagc tttggttatt cagacgtacg cgtacccggc aatcttccac aaggaagcca
      661 gggttgctgc tgttatcaaa gtcttgcgac aggctgaaga cggtgatttt gtctttgtac
      721 ggtttaccgt tccacggaca gaactgagcg cagttgccgc attcgttaca gtaagcgtcg
      781 aggtgcagcg tctggaaacg gttctggaag ccagggaccg caatggatac gttggcgcgg
      841 ttcgggcaga catccacaca cttgctgcaa acgtagttac attcaaggca gcgtgcggct
      901 tcctgggcga caaacgcatc acgatcgtca ctgttcacca gagtgacaga gatatcgcct
      961 ttacgttgat agatttccgc cggattgacg ttattccagt atttatcgtt ctggtgggaa
     1021 cggatatttt cccgactcag gatggtatcg gtcgcccgac gcgcggttcc gacagcagcg
     1081 acaatggagg atggtccgcg ctgtacgtcg ccgatcataa agacgtcact cagacgcgtt
     1141 tcgccattat ggtcgacgtc tggccagccg tttttgtcca gcggcacgcc catcgcattc
     1201 agggcttcag tatcctgctg ttcaccaatg gcggtgatca ggctgtctac atgcagtgtt
     1261 actgtttcgt tggtttcaac cggacggcga cgacctttct catcaggttc gccaagcgac
     1321 ataacgcgca aggttaaggt gccatcagca tcgaaacgtt ccggattatt caggaaacgg
     1381 aactccacgc cgtcgtgcaa cgcttcttca tactcttcgc gccatgctgg catctcttgc
     1441 agtgaacgac ggtaaacgac cgttgctttt tctacgcctg gaacgcgtaa cgccgcacga
     1501 gcgcagtcca ttgccgtgtt acccgccccg acaacgacca catgtttgcc cagcttgagc
     1561 gctgtgccct tgttgtattc acgcaggaag gggagtgatt tccagacatt ttggttgtcg
     1621 cccgccagtt tcacaccgct atttttatca gtgccggtgg caatcagaac atagtggaag
     1681 ccctgatttt ttaactgttc aacggtcaga tccggtgagc agccatactc aaattttacg
     1741 ccgtgagcgg caacaaaatc gatatcgtgc tgaattaact ctgcaggaat acggaactga
     1801 ggaatgatat ttttcaccac gccgcccgca ttggcttcgc gttcaaacag cgtaaccgga
     1861 tggcccgctc tggcaaggaa gtaacctgct gccagacccg ccggacctgc accaatcacg
     1921 gcaaccggat ggcgtgaacc agaaccggct ggtttgtgcc agcgttgctt atattcatcc
     1981 caaccttttt ccagcgcgac ttttttcagt tcgcggatat tcagcgcact gtcgtaatcc
     2041 aggcgggtac agttgtattg gcactggtga tcgcaaatat gaccggtaat ggcgggcagg
     2101 gcgttgcgtt gatagatgag ttccagcgcg tcggcatagc ggtgttcgcc aagcagacgg
     2161 atgtattccg gaatatcttg cttgatagcg caggcagtaa cacagggggc aacgtagcaa
     2221 tcggtcagcg gcaggtcttc agccacttca atacgctctt ctggcttcca gtgtttctgg
     2281 gtgtattcca tggttaacgc gtctgctgcc agtctgttca gtcgttcgac gtcaacgtgg
     2341 ttaagctccc aggcatctga gccttccagc tcgcgcatgc aggcacttaa gcgcagatag
     2401 ccgccaggtt tcagcaggtc ggttgccata gtaatagggc gaataccagt atcaaaaata
     2461 tcgcggatag tcagctgact ggcaccaccg gaataagaaa tgggcagttt gccgtcaaag
     2521 gcgcgagaga gaactgctgc aacgttgatg gagagcggga acagcgcacg gcctgacata
     2581 tacatctctt caccgggcaa tgcgccttta ttgttgatgg tgccgagagt gttagtcagt
     2641 tttacgccaa agccgagtga tttttctttt gccagtgcca tcaggcgttc cagcatttcc
     2701 agtgcttgcg tcagcttgag gtcgtgatca aatgactctt cttttaagcc gatgtaaccg
     2761 aaaccgcaga catcgaggat ctcacgaaca cgcgcgtacc ccagtaaggt cgggttgagt
     2821 ttcacaaagg tgttgagccc tttttcttcc agcatgtagc ggcaaatggc ttcgatttca
     2881 tgcggaggac agccgtgcat ggtggagagg gtaacccctt gtaccatgct ggtggggatg
     2941 cgagcgggta aggcttgcaa gctttcgcgt ttttcctgca atccgtggcg ggctaaaaat
     3001 gcctcattct ggagtagctt attaagtgca tcgcggtatt gagcgaattt cggatggtca
     3061 gatgcgtcca tcatattgtc gatgaactgc tgcatcggcg gttgcttaat accttcgagg
     3121 ttgtaaccga cgctcatatt aaagatgaac gatttaccgg aatcagaagg ctggaacatc
     3181 gcttcgagaa ggtgcagggc aaaccaggct ttgaggtatt catcccaggc tttaagcagg
     3241 gtaaattcgg tagaccattc ggtgttaaag cactcgtctt cggcatcgat acagggcttt
     3301 tccagctcca ggcggtcaag aatttggacg gtttttagtt cgatgaagcg tccgccagtc
     3361 agccaggagg tgacgatgtt ttgcgcaagt tgcgtgtgcg ggccagcggc agggccgacg
     3421 ggagtggcac aggtttcacc gaagacgcta acagttttac cttttacggg tgagtaaaac
     3481 tgttgctcgg gaataccaaa gattgagcgt tgttgttggt attcatcaaa tatgcgcgtc
     3541 aaaagttcct caaacggaat gggacgcata atatccccca taaccctctc cttagcttat
     3601 acagatatca ttgagtgacc ggtgttgaga aatccggtgg tttaggggga tagagcaaca
     3661 atcgcaccac tcttgccagg tttggcggga aatataaaaa aatgtgaggc ggctcgaaac
     3721 ttaatggctt tttgtgaatg aactcacagg tgccaaatga gaattaaaat cactcctgaa
     3781 aattattatt gccagcccat ttatcacttt ttctcttttc tcaaaatcag tgattcctta
     3841 tcattatgat aaagagtgct ggagggatta gatatttgaa cgcaatcgtt taactacgtt
     3901 ttctaaattt cagtatacct tttcttcgct gtaataaagt cgtccggagt atcaatatct
     3961 aaaattattt ctgcattttc tatttcgacg gaataatgtt ctcccataag gagtgcctga
     4021 cgcatattgg tgacattggg tcgctggatt gcctgcatca ggcatggttt tgacactaaa
     4081 atcggatggc cggggatgcc attatggagt ggcagtattg cgccatcatt tcgtaacgac
     4141 cagatttttc taaaaatatc gatggtgagg gttggcatat ctccgtgggt gagaaaacaa
     4201 tgttctgttt gcaccgcggg tactgcggcc tttactgatg tcagtaaacc ctgcgcataa
     4261 tctgggttgt ggataatagt gatattgctc tggttcacat aacgttcgtg cagttcatta
     4321 ccacgatagc cggtgactaa aataattcgg ctacaaaact gcaacgcatt tttgatactt
     4381 gtatcaagaa ttgttccctg ttgccagggt aacatcattt tccattgccc cattcttgat
     4441 gataatcctg cggcggtaat tatacagtcg atggctgaca tagtgattcc attatttaca
     4501 ggagctaata acgcctgata attgcgttac ttcctgatgg attacgttga gggtaattta
     4561 tcccagagag gtccataaag acgcgtgaaa agtataattg acccatcggc attattcatt
     4621 gatttaggcg cgcagaaacg tcccactgtg atttctattg ttggtgctgg ggggaaaacc
     4681 agcttgcttt tttggctggc agaactgttc caggcgagtg gcaggcgtgt attaatcact
     4741 acgacgacac atatgtttat gccaacatct cactggcccg tggttttctg tcgtgatccc
     4801 gccatgcttc ctcatgcgtc ttttatatcc cccatttcat tttgttttca ctgctggaaa
     4861 gcgaaccagg gaaaagtgca gggatttacg ccagaagcga ttgatgcact ggtacaacga
     4921 ccagagtgtg acgtaattct cattgaggca gatggctcgc gtggaatgcc gttaaaagcg
     4981 cctgatgagc acgaaccttg catacctaaa agcagttgct gcgtgattgc tgtgatggga
     5041 ggacatattt tgggtgcgaa agtgagcaca gaaaatgtcc atcgctggtc gcagtttgct
     5101 gatattactg ggttaacacc tgctgcaccc ttgcaactga gcgatctcgt tgcgctggtt
     5161 cgccaccctc agggggcgtt taaaaacgta ccacaaggtt gtcggcgagt ctggttcatt
     5221 aaccgttttt ctcaatgtga gaatgcgatt gcgcaaagcg agctccttca accgctgcaa
     5281 caacacaacg tagaggcaat ctggctgggc gatatacaag aacatcctgc aatcgcgcgc
     5341 agatttgtga attagcggcc cgggtattcc gggttactga aaggatatcc attgaggttg
     5401 gtatgaatat tttcacagag gctgcaaaac tcgaagagca aaattgtccg tttgcgatgg
     5461 cgcaaattgt tgatagccga ggctcaaccc cccgccattc tgcacaaatg ttagtgcgcg
     5521 ccgatggttc tatcgtcggt acaattggtg gcggaatggt tgaacggaag gtgattgaag
     5581 agtcgcttca ggcattgcag gaacgtaagc cgcgattatt ccatggacgt atggctcgta
     5641 acggtgcgga tgctgtcggg tcagattgtg gaggtgcaat gtcagtattt atcagcgtcc
     5701 atggtatgcg tccacgtctg gtgttgattg gtgcggggca tgtcaaccgg gcgatagccc
     5761 agagtgcggc gctattagga tttgatatcg ccgttgctga tatttatcgc gaaagcctca
     5821 atcctgaact attcccacca tcaaccacgc ttctccatgc tgagtcgttt ggtgcggcag
     5881 tggaagcact ggatattcgc cctgataatt ttgtcctgat tgccacgaat aatcaggatc
     5941 gtgaagccct cgacaaactc attgaacaac ccattgcatg gctggggttg ctggcaagtc
     6001 gccgcaaggt tcagcttttc ctgcgccaat tgcgtgagaa aggcgtggct gaagaacata
     6061 ttgcccgttt acatgcgccc gttggttaca acataggtgc ggaaacgccg caggagatcg
     6121 ccatcagcgt gctggcagaa atattacagg tgaaaaataa cgcgccgggt gggctgatga
     6181 tgaagccttc tcatccttcc ggacaccagc tggtggtgat tcgcggtgcg ggggatatcg
     6241 ccagtggtgt agcgctacgt ctgtatcatg cgggttttaa agtgatcatg ttggaagtgg
     6301 aaaaaccgac agtgattcgt tgtaccgtgg cgtttgccca ggccgtgttc gatggcgaaa
     6361 tgacggtcga aggcgtcacc gcccgcctgg caaccagctc tgcggaagcg atgaaactta
     6421 ccgaacgcgg atttatccct gtgatggtag atcccacctg ttcattgctt gatgaactga
     6481 aaccgctttg cgtggtggac gctattctgg cgaaacagaa tttgggaacg cgggcagata
     6541 tggcaccagt aacaatcgcg cttgggccgg gctttactgc agggaaggat tgtcatgcgg
     6601 taattgaaac aaatcgcggg cactggctcg gtcaggtgat ttactcaggt tgtgcgcagg
     6661 agaataccgg tgttcccggc aatattatgg ggcataccac ccgacgagtg atccgcgctc
     6721 ctgctgcagg cattatgcga tccaacgtga aattaggcga tctggtgaaa gagggcgatg
     6781 tcattgcctg gattggtgag catgaaatta aagcaccgtt gacggggatg gtgcgtggct
     6841 tgttgaacga cgg
//
LOCUS       DAWDRC010000152         6836 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5229_length_6836_cov_2.161923, whole genome shotgun sequence.
ACCESSION   DAWDRC010000152 DAWDRC010000000
VERSION     DAWDRC010000152.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5229_length_6836_cov_2.161923"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1426..1435
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2238..2247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2530..2539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4462..4471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5171..5180
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1726 a         1777 c         1646 g         1637 t
ORIGIN      
        1 agcgaaaaaa gccgccagta aaagtacgac caaaagcaaa accgcttctt ccgttaaaaa
       61 atcttccatt accgcttcta aaaacgccaa aactcgcagc aaacacgccg tcaataaaac
      121 ggcctcagcc agctttaccg aaaagtgtac caaacgtaag ggctataaat cgcattgtgt
      181 aaaagtcaaa aatgccacgt caggaaccct tgctgacgcg cacaaagcga aggtgcaaaa
      241 agccacaaaa gtggcaatga ataaactgat gcagcaaatt ggtaagccat atcgttgggg
      301 tggcagttca ccgcgtaccg gttttgattg cagcggtctg gtttattacg cttataaaga
      361 tttggtgaaa attcgtattc cgcgtacggc gaatgaaatg tatcacctgc gcgatgcagc
      421 gccaatcgaa cgtagtgaac tgaaaaacgg cgacctggtc ttcttccgta ctcagggacg
      481 cggtacagcc gatcatgtcg gcgtgtatgt cggcaacggc aaatttattc agtcaccgcg
      541 cacgggccaa gaaattcaaa tcacttctct cagtgaagac tactggcagc gccactatgt
      601 tggcgctcgc cgggtaatga ccccaaaaac acttcgctaa aactttaccc tgttgttacg
      661 gcaacagggt aagttcatct tttgtctcac cttttaattt gctaccctat ccatacgcac
      721 aataaggcta ttgtacgtat gcaaattaat aataaaggag agtagcaatg tcattcgaat
      781 tacctgcact accatatgct aaagatgctc tggcaccgca catttctgcg gaaaccatcg
      841 agtatcacta cggcaagcat catcagactt atgtcactaa cctgaacaac ctgatcaaag
      901 gtaccgcgtt tgaaggtaaa ccactggaag agattattcg cagctctgaa ggtggcgtat
      961 tcaacaacgc agctcaggtc tggaaccata ctttctactg gaactgcctg gcaccgaacg
     1021 cgggtggcga accgactgga aaagtcgctg aagctatcgc cgcatctttt ggcagctttg
     1081 ccgatttcaa agctcagttt actgatgcag cgatcaaaaa ctttggttct ggctggacct
     1141 ggctggtgaa aaacagcgat ggcaaactgg ctatcgtttc aacctctaac gcgggtactc
     1201 cactgaccac cgatgcgact ccgctgctga ccgttgatgt ctgggaacac gcttattaca
     1261 tcgactatcg caatgcacgt cctggctatc tggagcactt ctgggcgctg gtgaactggg
     1321 aattcgtagc gaaaaatctc gctgcataat aactgatggc aaatgcaaca ttatctgatg
     1381 cgcttcgctt accatgccaa cgcggatgag gcgttaacag cgcatnnnnn nnnnngcaac
     1441 tgtaacgcag aaggttatcc ttctgcgttt ttgtttaatt agctgttggc aacgcaaact
     1501 gcttcaggtt gttttctggc tgacataaac accagcaata atgccagtcc cgcgacaatc
     1561 gcccccatca ccggcacaaa actgtatccc agcccagcgg aaattaccgc accaccagca
     1621 gcggctccca gcgcatttcc aagattaaag gcaccaatat tgactgatga agacagacct
     1681 ggcgcttcac tggcgacacg catcacgcgc atctgtaacg gcggtacgac cgcaaaggtt
     1741 gcagctcccc acaccaccat gctcatagct gcgccgaact cattgcgggc caggaacggg
     1801 attgccagca taatcaccat cagcaacaac aaaaagcctt tcaacgtgcc gttaactgaa
     1861 cgatctgcca gtttgccgcc gagatagtta ccgatagaaa atccgacacc aatcagcacc
     1921 agcattgccg tgacgaacac cggcgttgcg tgggtaatac tttgcagtac cggagagata
     1981 taggtgtaga gggtaaacat tgcaccagct cccagtaccg tcgtcagcaa tgcagacagc
     2041 acctgcggac gcattaatac cgccagctct tttttcactt caggtcgtgc ccctgcacca
     2101 cctttaggta atgagaagaa cagacttacc attgaaatca ctcccagccc cgccgttgcc
     2161 agaaatgaca tccgccagcc gatggtttca cccaaccagg tagccgccgg cacgccaccg
     2221 atacttgcca gggttaannn nnnnnnncat cagggtcata taatccggcg cgatggcgga
     2281 aagtacgttg ccaagcgtga aaatcgccat caggaaaatc aacgcactgc ggcgggcacg
     2341 atgagaaagt agaagcgtca tcagcggcgc gcccaccatt acgccaactg cataggcact
     2401 gattaacatt ccggcagcgg gaatcgagac atccacaccg cgcgcaatga cgggcaacaa
     2461 gcccattggc gagaactccg ttgtcccgat accaaacgcg ccaatcgcca gcgccagcaa
     2521 cggaaagttn nnnnnnnnna aaatgaaaat aacaaaaaga gaaaacactt ttgccatttt
     2581 gctaacaaac aggaaggaga tgcgagggag aacgcgctcc ctcgagagga aatcagtgca
     2641 gcgcggcagt caaacccacg gccacgatca aaccgaggac gataatcgtt gttaccagtg
     2701 aaaatttaag gtcggtgctc atcaagtttt ctcctttttt attaccacac aaaaagtgat
     2761 attacgcatt tttacacact gtgatgaaaa aatctcccgt catttataat gataagtgtt
     2821 tttaccactt ccccttttcg tcaagatcgg ccaaaattcc acgcttacac tatttgcgta
     2881 ctggccattg accccttcct gacgctccgt gtcgtttttc cggcgtaccg caacactttt
     2941 gttgtgcgta aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg agctgaagtt
     3001 agggtctgga gtgaaatgga atggcaacaa taaaagatgt agcgaaacga gcaaacgttt
     3061 ccactacaac tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa gaaacgcgca
     3121 acgccgtgtg ggcagcgatt aaagaattac actactcccc tagcgcggtg gcgcgtagcc
     3181 tgaaggttaa ccacaccaag tctatcggtt tgctggcgac cagcagcgaa gcggcctatt
     3241 ttgccgagat cattgaagcg gttgaaaaaa attgcttcca gaaaggttac accctgattc
     3301 tgggcaatgc gtggaacaat cttgagaaac agcgggctta tctgtcgatg atggcgcaaa
     3361 aacgcgtcga tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg ctggcgatgc
     3421 tggaagagta tcgccatatc ccaatggtgg tgatggactg gggtgaagca aaagctgact
     3481 tcaccgatgc ggtcattgat aacgcgttcg aaggcggcta catggccggg cgttatctga
     3541 tagaacgcgg tcaccgcgaa atcggcgtta tccctggccc gctggaacgt aacaccggcg
     3601 caggccgcct tgccggtttt atgaaggcga tggaagaagc gatgatcaaa gtgccggaaa
     3661 gctggattgt acagggtgat tttgaacctg aatccggtta tcgcgccatg cagcaaatac
     3721 tgtcgcaacc gcatcgccct actgccgtct tctgtggtgg cgatatcatg gcaatgggcg
     3781 cactttgtgc tgctgatgaa atgggtctgc gcgtgccgca ggatgtttcg ctgatcggtt
     3841 atgataacgt gcgcaacgcc cgctatttta cgccggcgct gaccacgatc catcagccaa
     3901 aagattcgct gggcgaaacg gcgttcaaca tgctgttgga tcgtatcgtc aataaacgcg
     3961 aagaaccgca atccattgaa gtgcatccgc gattgattga acgccgctcc gtggctgacg
     4021 gcccgttccg cgactatcgt cgttaatcac ccgttgcggg agtctcttcc ggctcccgca
     4081 gccactcctt attcagcgtc tcactatcgc cgagatactc aagcaaccag gttaacgcag
     4141 gcgacatatc attttgctgc catgtcagac aacatgccga atccgggaaa gggttttcca
     4201 gttctagtgc tacccactta ccctcattaa gccacggttt ggcgaaatgt gttggcacca
     4261 tccctatgca taatcctgca gagatacagg ttgccgatga ttcccagtca ggcacgacaa
     4321 gtcttttttg gttatccagc aaccaggtaa tacgtttagg tagcgttcgc gaggtgtctt
     4381 cgcgcaccaa cgacggccag ttgcgcaacg tatcatcgct gaacgggcca tccatcaacg
     4441 ccagcgggtg gtggctggca annnnnnnnn naaacaacgc aaatccagct taacatcccc
     4501 atatcccgga aggcataacg accgcctacc ggaatcgcac gtgttgcgcc aatcgccagt
     4561 tccacgcgcc cgtcggaaag tgcatcccag acaccgttga acacttcctg aaagacaaga
     4621 agttcgacat catcaaaatg gcgataaaaa tcaacgatca tctgccgtgt tcgctctggc
     4681 ctgacaatat tatccactgc gatagctaac tgaccgcgcc agccgttcgc tatctgctga
     4741 cattgctggc gggtgatctg catttttttg acaacagagc gtccttcttt gagaaaccac
     4801 gccccggcag cggtcagttc cacatcacgg tgacgtcgtt caaaaagcgg caccgccagc
     4861 cactcttcca gctgacgcac ggtatagctg accgcagaag gaacgcgatg caactcctgg
     4921 gccgcggcgc taaaactacc attacgcgct accgcatcaa caacttccag agaatattct
     4981 gaccacatag tctgcctgca aaatttttga aaccaatcat caaatattac cgtttcacaa
     5041 cactaatttc actccctaca ctttgcggcg gtgtttaatt gagagattta gagaatatac
     5101 atgcaacctg ggaaaagatt tttagtctgg ctggcaggtt taagcgtact cggttttctg
     5161 gcaaccgata nnnnnnnnnn gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt
     5221 ctgctgtcag tgccagcctt agtctgttcc ttgccggttt tgccgcagcc cagcttctgt
     5281 gggggccact ctccgaccgt tatggtcgta aaccggtgtt attcatcggc ctgacaattt
     5341 ttgcgttagg tagtctgggg atgctgtggg tagaaaacgc cgccacgctg ctgatattgc
     5401 gttttgtaca ggctgtgggt gtctgcgccg cggcggttat ctggcaagca ttagtgacag
     5461 attattatcc ttcacagaaa gttaaccgta tttttgcgac catcatgccg ctggtgggtc
     5521 tgtctccggc actggctcct ctgttaggaa gctggctgct ggtccatttt tcctggcagg
     5581 cgattttcgc caccctgttt gccattaccg tggtgctgat tctgcctatt ttctggctca
     5641 aacccacgac gaaggcccgt aacaatagtc aggatggtct gacctttacc gacctgctac
     5701 gttctaaaac ctatcgcggc aacgtgctga tatacgcggc ctgttcagcc agtttttttg
     5761 catggctgac cggttcaccg ttcatcctta gtgaaatggg ttacagtccg gcagttattg
     5821 gtttaagtta tgtcccgcaa actatcgcgt ttctgattgg tggttatggc tgtcgtgccg
     5881 cgctgcagaa atggcaaggc aagcagttat taccgtggtt gctggtactg tttgctgtca
     5941 gcgtcattgc gacctgggct gcgggcttca ttagccatgt gtcgctggtc gaaatcctga
     6001 tcccattctg tgtgatggcg attgccaacg gtgcgatcta cccgattgtt gtcgcccagg
     6061 cgctgcgtcc cttcccacat gcaaccggtc gcgccgcagc gttgcagaac actctgcaac
     6121 tgggtctgtg cttcctcgca agtctggtag tttcctggct tatcagtatc agcacgccat
     6181 tgctcaccac caccagcgtg atgttatcaa cagtagtgtt ggtcgctctg ggttacatga
     6241 tgcaacgttg tgaagaagtt ggctgccaga atcatggcaa tgccgaagtc gctcatagcg
     6301 aatcacactg atctatatcg atatacttat acttaggctg ctaacaaaat tttgttgtat
     6361 gattgaaatt agcggcctat actaatttcg agttgttaaa actacgataa atattatgtt
     6421 attacgggga caggatcgtt cccgactcac tatggatggt catttcggca agggttcctc
     6481 ctttccctct gttctacgtc ggattataga ctcgcggttt tttctgcgag atttctcaca
     6541 aagcccaaaa agcgtctacg ctgttttaag gttctgatca ccgaccagtg atggagaaac
     6601 tatgagttca tcgtgtatag aagaagtcag tgtaccggat gacaactggt accgtatcgc
     6661 caacgaatta cttagccgtg ccggtatagc cattaacggt tctgccccgg cggatattcg
     6721 tgtcaaaaac cccgattttt ttaaacgcgt tctgcaggaa ggttctttgg ggttaggcga
     6781 aagttatatg gatggctggt gggaatgtga ccgactggat atgtttttta gcaaag
//
LOCUS       DAWDRC010000153         6818 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5248_length_6818_cov_2.401597, whole genome shotgun sequence.
ACCESSION   DAWDRC010000153 DAWDRC010000000
VERSION     DAWDRC010000153.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5248_length_6818_cov_2.401597"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1667 a         1644 c         1808 g         1699 t
ORIGIN      
        1 actgaccggg ccctttgaaa gcattgttga gatgtcatgc ctgatgcacg atatcggcaa
       61 tccgccgttt ggtcattttg gcgaagcggc gataaatgac tggtttcgcc agcgtttgta
      121 cccggaagat gccgaaagcc agcctctgac tgacgatcgc tgcagcgtgg cggcactacg
      181 tttacgggac ggggaagaac cgcttaacgc gctgcggcgc aagattcgtc aggacttatg
      241 tcattttgaa ggtaacgcac agggtattcg tttggtgcat acattgatgc ggatgaatct
      301 cacctgggca caggttggcg gtattttaaa atatacccgt ccggcgtggt ggcgtggcga
      361 aacgcctgag acacatcact atttaatgaa aaagccgggt tattatcttt ctgaagaagc
      421 ctatattgcc cggttgcgta aagaacttaa tttggcgctt tacagtcgtt ttccgttaac
      481 gtggattatg gaagctgccg acgacatctc ctattgtgtg gcagaccttg aagatgcggt
      541 agagaaaagg atatttaccg ttgagcagct ttatcatcat ttgcacgagg cgtggggcca
      601 gcatgagaaa ggttcgctct tttcgctggt ggttgaaaat gcctgggaaa aatcacgctc
      661 aaatagttta agccgcagta cggaagatca gttttttatg tatttacggg taaacaccct
      721 aaataaactg gtaccctacg cggcacaacg atttattgat aatctgcctg cgattttcgc
      781 cggaacgttt aatcatgcat tattggaaga tgccagcgaa tgcagcgatc ttcttaagct
      841 atataaaaat gtcgctgtaa aacatgtgtt tagccatcca gatgtcgagc agcttgaatt
      901 gcagggctat cgggtcatta gcggattatt agagatttat cgtcctttat taaacctgcc
      961 gttatcagac tttactgaac tggtagaaaa agaacgggtg aaacgtttcc ctattgaaac
     1021 gcgcttattc cacaaactct cgacgcgcca tcggctggcc tatgtcgagg ctgtcagtaa
     1081 attaccgtca gattctcctg agtttccgct atgggaatat tattaccgtt gccgcctgct
     1141 gcaggattat atcagcggta tgaccgacct ctatgcgtgg gatgaatacc gacgtctgat
     1201 ggccgtagaa caataaccag gcttttgtaa agacgaacaa taaattttta ccttttgcag
     1261 aaactttagt tcggaacttc gcgctataaa acgaatctga agaacacagc aattttgcgt
     1321 tatctgttaa tcgagactga aatacatgaa aaaaaccaca ttagcactga gtgcactggc
     1381 tctgagttta ggtttggcgt tatctccgct ctctgcaacg gcggctgaga cttcttcagc
     1441 aacgacagcc cagcagatgc caagccttgc accgatgctc gaaaaggtga tgccttcagt
     1501 ggtcagcatt aacgtagaag gtagcacaac cgttaatacg ccgcgtatgc cgcgtaattt
     1561 ccagcagttc ttcggtgatg attctccgtt ctgtcaggag ggttctccgt tccagagctc
     1621 tccgttctgc caggggggcc tgggcggtaa tggcggcggc cagcaacaga aattcatggc
     1681 gctgggttcc ggcgttatca ttgatgccga taaaggctat gtcgtcacca acaaccacgt
     1741 tgttgataac gcgacggtga ttaaagtcca actgagcgat ggtcgtaagt tcgacgcgaa
     1801 gatggttggc aaagatccgc gctctgatat cgcgctgatc cagatccaga acccgaaaaa
     1861 cctgaccgca attaagatgg cggattctga tgcgctgcgc gtgggtgatt acaccgtagc
     1921 aattggtaac ccgttcggcc tgggcgagac agtaacttcc gggattgtct ctgcgctggg
     1981 acgtagcggc ctgaacgctg aaaactacga aaacttcatc cagaccgatg cagcgatcaa
     2041 ccggggtaac tcaggtggtg cgttggttaa cctgaacggc gaactgatca acaccgcgat
     2101 cctcgcaccg gacggcggaa acatcggtat cggttttgct atcccgagca acatggtgaa
     2161 aaacctgacc tcgcagatgg tggaatacgg ccaggtgaaa cgtggtgagt tgggtattat
     2221 gggcactgag ctgaactccg atctggcgaa agcgatgaaa gttgacgccc agcgcggtgc
     2281 tttcgtaagc caggttctgc cgaattcttc cgccgcgaaa gcgggcatta aagcgggtga
     2341 tgtgatcacc tcactgaacg gtaagccaat cagcagcttt gccgcactgc gtgctcaggt
     2401 gggcactatg ccggtaggta gcaaactgac cctgggctta ctgcgcgacg ggaagcaggt
     2461 taacgtgaac ctggaacttc agcagagcag ccagaatcag gttgattcca gcaccatctt
     2521 caacggcatt gaaggcgctg agatgagtaa caaaggcaaa gatcagggcg tggtggtgaa
     2581 caacgtgaaa acgggcactc cggctgcgca gatcggcctg aagaaaggtg atgtgattat
     2641 tggcgcgaac cagcaggcag tgaaaaacat cgctgaactg cgtaaagttc tcgacagcaa
     2701 accgtctgtg ctggcactga acattcagcg cggcgacagc accatctacc tgttaatgca
     2761 gtaatctccc tcaacccctt cctgaaaacg ggaaggggtt ctccttacaa tctgtgaact
     2821 tcaccacaac tccatacatc ttcatcatcc tttgggcatt tgcacaatgc cgtacgttac
     2881 gtacttcctt atgctaagcc gtgcataatg gaggacttat ggctggctgg catcttgata
     2941 ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat catcgatacc aatatcaacg
     3001 taatggatgc ccgcgggcga attatcggca gcggcgatcg tgagcgtatt ggtgaattgc
     3061 acgaaggagc attgctggta ctttcacaag gacgagtcgt cgatatcgat gacgcggtgg
     3121 cacgtcatct gcacggtgtg cggcagggga ttaatctacc gttacggctg gaaggtgaaa
     3181 ttgtcggcgt aattggcctg acaggtgaac cagagaatct gcgtaaatat ggcgaactgg
     3241 tctgcatgac ggctgaaatg atgctggaac agtcgcggtt gatgcacctg ttggcgcagg
     3301 atagccgttt gcgggaagaa ctggtgatga acctcattca ggctgaggag aatactcccg
     3361 cacttactga atgggcgcaa cggctgggga tcgatctcaa tcaaccgcga gtggtggcta
     3421 ttgttgaggt cgacagcggt cagcttggcg tggacagcgc aatggcggag ttacagcaac
     3481 tgcaaaatgc gctgactacg cccgagcgta ataatctggt ggcgattgtc tcgctaaccg
     3541 aaatggtggt gttgaaaccg gcgttgaact cttttgggcg ctgggacgca gaagatcatc
     3601 gtaagcgagt tgaacaactg attacccgca tgaaagagta tggccagctg cgttttcgcg
     3661 tttcgctggg caactatttt accggtcctg gcagtattgc ccgatcctat cgtacggcga
     3721 aaacgacgat ggtggtgggt aaacagcgga tgccagaaag tcgctgctat ttttatcagg
     3781 atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga ctggcaggcc aacgaactgg
     3841 cgcgaccgct ggcgcggctg aaagcgatgg acaataacgg cttgctgcgg cgaactctgg
     3901 cggcgtggtt tcgccacaat gtgcaaccgc tggcaacgtc aaaggcgttg tttattcatc
     3961 gtaatactct ggaatatcgg cttaatcgta tatcggaact gaccgggctt gatttgggca
     4021 attttgatga caggttgctg ctgtatgtgg cgttacaact ggatgaagag cggtaggtcg
     4081 attacaaaat tacagccggg tgagacttta cccggcccac agaatggcat tatttacgcg
     4141 tcagtttttc cagatcggct tcaatctcgc tgatcttatt ggttactact gactccaggt
     4201 gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca cgctggcaga
     4261 tttgatcaag ctcatcaatg atataccgta gattcgggct aatttcctgg acttctttat
     4321 aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcgggtat ttaaacttca
     4381 cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga atatcgttgt
     4441 tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata cccagacttt
     4501 tcagattgtc gtacatagtg ttacctcaaa atgagtcagt aaattgtgct tattttagca
     4561 tttggcctgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt ccagacttct
     4621 atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gtgatattcc atggaatgga
     4681 ttagcacatt tatcttcatt ttgcgaatta catgacagtc cgaaacaaga tgaaaaagta
     4741 tttaaaaaag ggggaaaatg agtaggacga attattctga gccagaaagc aatcgggcag
     4801 cgtcgtgctg cccgtgtaca tacttttagt cgatggtacg cagcagttcg ttaataccaa
     4861 ctttaccgcg agttttcgcg tcaactttct taacgataac tgcacagtag agactgtatt
     4921 tgccatcttt cgacggcaga ttacctgaaa caaccacaga ccctgccgga acgcgaccgt
     4981 agtggatttc gccggtttca cggtcataaa tacgggtgct ctgaccaatg tatacgccca
     5041 tagagatgac ggaaccttct tcgacaatca ccccttcaac cacttcagag cgtgcgccga
     5101 tgaagcagtt atcttcaata atggtcgggt tagcctgcag cggctccagc acgccgccga
     5161 tacccacgcc accggaaagg tggacgtttt taccaatctg cgcacaggaa ccgacggtcg
     5221 cccaggtatc aaccatggtg ccttcatcaa catatgcgcc gatgttgacg taagacggca
     5281 tcagcacggt gttacgggca atgaacgcgc cctgacgcac tgccgctggt ggcacgacgc
     5341 ggaagccttc tttctggaaa cgtgcttcat cgtagttagc gaatttcatc ggcactttgt
     5401 cgaagtagcg gctttctgcc ccttcgatca cctgattatc attaatacgg aaagagagca
     5461 gcaccgcttt tttcaaccac tgatgcgtca cccactgacc gtcaattttt tccgctacac
     5521 gcagtgcgcc ggaatccagc agggcgatca cctgatttac cgcttcgcgg gtaacggtgt
     5581 ctgcattggc tggcgtgatc tcggcacggc gttcaaaagc ggtttcaata atgttctgta
     5641 actgctgcat tgttaaactc ttttcatatc agtaaacaca tcacccttta tcgtttggat
     5701 tgagggcctc tgtcaaccgc tgatgcactt cctgctgcaa ctcgttatta agcgcacgcc
     5761 ggtcagcggt ggcaattatg aataaatctt ctactcgctc gccaatggtt gtaattcggg
     5821 ctccatgaag cgaaattccc agatcggcaa aaattttccc gactcgcgcc agcagtccag
     5881 gttggtcgag ggcgatcagt tcgaggaacg atttgcggtc ggtatgggtc ggcaaaaacg
     5941 tcacttcggt ttcaacagta aaatggcgca atttggcggg ttggcgacgg ggctgcggtg
     6001 gctgccagct actttgtatc agtacttgct ccagaccaaa ccgaataacc tcatgacgat
     6061 ctgcggatag cgggctacca tcgggttcca gcactataaa ggtatccatc gccataccgt
     6121 cgcgagtggt gaaaatttgt gcgtcgtgaa cacttaaatt gcggcggtct aattcggcac
     6181 agacggcggc aaacagatag gggcggtccg ggctccagat aaaaatctcg gtgcctccac
     6241 gcgtagcctg cgggctaagc agtaccagcg gtttgcttaa atcatgctgt aataaatggc
     6301 gtgcgtgcca ggccagttga tttgggctat ggcggacaaa atagttagcg cgacagcgtg
     6361 accaaatttg gtgcagcgcc tcttcgtcga tgttatccat gcgcagtagt gccagcgcct
     6421 ggagttggtg atggcgaacc cgttcgcgca tatccggcgt gttttgcatt ccgcgtcgta
     6481 gctgcttttc ggtggcaaaa tagagttcgc gcaacagact ttgcttccag ctattccaca
     6541 gtgtttcgtt ggtggcgcaa atgtcagcca ctgtcaggca taccagatag cgcagacgat
     6601 tttccgtttg aacttcttcg gcaaactgct tgatgacttc cgggtcctga atatcgcggc
     6661 gctgagcggt caccgacatc agcaagtgct ggcgaaccag ccaggcgacc aactgtgttt
     6721 cgcgtgagtt cagcccgtgg agttcggcaa aatgcactac atcctgagca ccgagaatgg
     6781 agtggtcgcc gccgcgtcct ttggcgatat cgtgaaac
//
LOCUS       DAWDRC010000154         6817 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5249_length_6817_cov_2.039485, whole genome shotgun sequence.
ACCESSION   DAWDRC010000154 DAWDRC010000000
VERSION     DAWDRC010000154.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5249_length_6817_cov_2.039485"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    445..454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2866..2875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4480..4489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5821..5830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1656 a         1659 c         1701 g         1761 t
ORIGIN      
        1 ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt
       61 ttacccatac agaacagcgc gtcatcaacc gcatgttaaa gctggcgctt gaaggctata
      121 gcgatgcctg gaaggcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg
      181 tgaaatttac caatatcacc acctcaccaa atgacatcgt ggttaacaca ccgttccatg
      241 tggagattgg caacctgacc ggcgaattta atatctgcct gccattcagc atgatcgagc
      301 cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact
      361 ggcgcgataa cctggtgcgc caggtgcagc attcacagct ggaactggtg gacaactttg
      421 ccgatatctc gatacgcctg ttccnnnnnn nnnnatttta aaactgaaac ccggcgacgt
      481 cctgccgata gaaaaacccg atcgcatcat cgcccatgtt gacggcgttc cggtactgac
      541 cagtcagtac ggcaccctca acggtcagta tgcgttacgg atagaacatt tgattaaccc
      601 gattttaaat tctctgaacg aggaacagcc caaatgagtg acatgaataa tccggccgat
      661 gacaacaacg gcgcaatgga cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc
      721 agcggaaaaa gcgctgccga cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga
      781 acgttgcagg atatcgacct gattatggat attccggtca agctgaccgt cgagctgggc
      841 cgtacgcgga tgaccatcaa agagctgttg cgtctgacgc aagggtcagt cgtggcgctg
      901 gacggtctgg cgggcgaacc gctggatatt ctgatcaacg gttatttaat cgcccagggc
      961 gaagtggtgg ttgttgccga taaatatggc gtgcggatca ccgatatcat taccccgtcc
     1021 gagcggatgc gccgcctgag ccgttagtga tgaataacca tgctactgtg caatcttccg
     1081 cgccggtttc tgctgagcca ctgctgcagg tgagcggcgc actcatcgcc attattgccc
     1141 tgatcctcgc tgctgcctgg ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg
     1201 ttaacggtct gaaaattagc gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg
     1261 tcgatgtgga agatgcacgg ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc
     1321 ataaacttcc cccttcagca ccaacggaag aggtaccgca aaccgatttt cagtcggtca
     1381 tgaaaaattt gcttaagcgt ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc
     1441 ggtccttctc tggctgatta cgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca
     1501 gccgctacct ggcggtggac aaagctggtc gctcccggtg cagacgctgg tgttcatcac
     1561 ctcgttgacg ttcattccag cgattttact gatgatgacc agtttcaccc gcatcattat
     1621 tgtttttggt ttattgcgta acgcgctggg aacgccctcc gcgccaccta accaggtatt
     1681 gctggggctg gcactgtttt tgaccttttt tattatgtcg ccggtgatcg acaaaattta
     1741 tgttgatgcg taccagccat tcagcgaaga gaaaatatca atgcaggagg cgctggaaaa
     1801 aggggcgcag ccgctgcgtg agtttatgct gcgtcagacc cgtgaggcag atttagggtt
     1861 gtttgccaga ctggcgaata ccggcccgtt gcagggacct gaagccgtgc cgatgcgcat
     1921 tttgctcccg gcctacgtga ccagcgagtt gaaaaccgca tttcagatag gcttcacgat
     1981 tttcatccct tttttgatta tcgacttggt gattgccagc gtgttgatgg cattggggat
     2041 gatgatggtc ccccccgcca ccattgctct gccctttaaa ctgatgctgt ttgtactggt
     2101 ggatggttgg caattgctgg tcggttcgct ggcgcagagc ttttacagct agagaggcaa
     2161 aatgacacct gaatcggtca tgatgatggg gactgaagcg atgaaagtcg cgctggcact
     2221 ggctgccccg ctgttgctgg tggcgctggt cacgggtctt atcatcagta ttttgcaggc
     2281 cgccacccag attaacgaaa tgacgctgtc gtttattccg aaaatcatcg ccgtatttat
     2341 cgccattatt attgccggac cgtggatgct caatctgttg ctggattacg tccgcacctt
     2401 gttcactaac ctgccgtata tcatcgggta gcccgaacaa tgatgcaggt gacaagcgat
     2461 caatggcttt cctggttaag cctgtacgtc tggccgttac tgcgcgtgct ggcgctcatt
     2521 tctaccgcgc caattctgag cgaacgcagc gtgccgaaac gggtaaaact gggtctggca
     2581 atgatgatca cgttcgccat tgccccatca ttacctgcca acgatgttcc tgttttttcg
     2641 ttctttgctc tgtggctggc cgtgcagcag atcctgatcg gcattgcgct tggttttacc
     2701 atgcaatttg cctttgccgc tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg
     2761 ctgtcatttg cgacatttgt cgatcctggc agccatctca atatgcccgt tttagcgcgc
     2821 atcatggata tgctggcatt actgctgttc ctgacattta acggtnnnnn nnnnntcact
     2881 gctggtcgat acctttcaca ccctgccgat tggtggtgaa ccgttgaaca gcaatgcgtt
     2941 tctggcactt accaaagcag ggagtttgat tttccttaac gggctgatgc tggcgttacc
     3001 gctcattact ctgttgctga ccctgaatct ggcattaggt ttactgaatc gtatggcccc
     3061 gcaattatcc atttttgtta ttggatttcc attaactctg actgtcggca tctctttaat
     3121 ggcggcatta atgccgttaa ttgcgccttt ttgcgaacat ttattcagtg aaatttttaa
     3181 tttgctggct gatattatta gtgaattacc attaatataa ttccgtaacg tttatcatgc
     3241 taccctaagg attatccgaa aaataatatc tgcgaacatc ttccaggata ctcctgtagc
     3301 gaaatatttg ttttaagctc actcacatat cgcaacattt actttacttt aagacaattc
     3361 caggcaaatt atacaacact ttacgggata gtaagtccgc ctgaaaaatt gcgagagtgg
     3421 cgcattaggt gacccatgtt gttccgttta gtcatgatga aatattcagg taaggggaat
     3481 tatcgttacg cattgagtga gggtatgcca tgtcaacgat tattatggat ttatgtagtt
     3541 acacccgact aggtttaacc gggtatctgt tgagtagagg ggttaaaaaa agagaaatca
     3601 acgacattga aaccgttgat gaccttgcca tagcttgtga ttcacagcgc ccttcagtgg
     3661 tgtttattaa tgaggactgt ttcatccacg atgcttctaa cagtcagcgc atcaagcaca
     3721 tcattaatca acatcccaat acgttattta tcgtttttat ggcaattgcc aatgttcatt
     3781 ttgatgaata tctattggtc agaaaaaatt tattgatcag ttctaaatcg attaaaccgg
     3841 aatctctcga cgatatcctt ggcgatattc tgaaaaaaga gacaacgata acctcgtttt
     3901 taaatatgcc gacgttatca ttgagccgaa ccgaatcgag tatgttgcga atgtggatgg
     3961 caggtcaggg aaccattcaa atctccgacc aaatgaatat caaagccaag accgtttcat
     4021 cgcataaagg taatattaaa cgtaagatca aaacgcataa taaacaggtt atctaccatg
     4081 tcgtccgact gacggataat gtgactaatg gtatttttgt caacatgcgc taacacattc
     4141 tgactggtgg tttcccacca gtcaggctga ataagattac tctgctttct caacaaagat
     4201 accgtcctga tgccctgctt cattaaagaa ccagatgccg agcgggtagt cttccagcga
     4261 aaccaggtac attgtgcctt cactaaactc ctcaactgcc agtaccacgc caggacgacg
     4321 cggaccgcca tccgttttga ctgttacccg atcattcacc ttcatcgttt tcctcctgtg
     4381 gctttgtgcc agtgtagaac aatttcgttt tcctggcagc gccaggcgcg cgcgagtgct
     4441 gattttctcg acggtctata cttaagagat gccaggcggn nnnnnnnnng acagttgatg
     4501 aagcctgtga aggaatggca aaggatgtcg atgccctgct ggcggcgatc aatgaaatta
     4561 gcgaaagcga agttcatcgc agccagaacg attctgaaca cgttagcgtc gatggacgtg
     4621 aatatcatac atggcgtgaa ttggcggatg ccttcgaact ggatattcat gacttcagcg
     4681 tctctgaagt gaatcgttga atgcacaata aaaaaatccc gaccctgagg gggtcgggat
     4741 gaaacttgct taagcaagaa gcacttaaaa aattcgttac accaggaaat ctgatgtgtt
     4801 catcacctta tccgcaattt ttttcgctga caagaaaata ttcgcgctat gaatgattaa
     4861 cgctacttat gaatagaaat gtgacgcgcg tcaccccttg gtggctctgg tgtcaacggg
     4921 atcgctcccg aaaaaatcat ctttgattat ttgtgctgtt ttgagaatca tcctggtaag
     4981 gggagtactg cgcgaccatg ttttcaattc aacaaccact actggttttt agcgatcttg
     5041 atggcaccct gctgaacagt catagttatg actggcaacc ggcagccccc tggctcagcc
     5101 gtttacacga agccaatgtt cccgtcattc tctgtagcag taaaacttca gcggaaatgc
     5161 tgtacttgca aaaaatgttg gggttacaag gcttaccgct gattgcagag aatggcgcag
     5221 tgatccagct tgctgaacaa tggcaggata tagacggttt tccacgcatc atctcaggta
     5281 ttagccatgg cgaaatctgc caggttttaa atacgctacg cgagaaagaa cattttaaat
     5341 tcacgacttt tgatgatgtc gacgatgcaa ccattgccga atggacggga ttaagccgta
     5401 gccaggcggc gctgacgcag ctgcatgagg cgtcggtaac gctaatctgg cgcgacagtg
     5461 acgagcatat ggcacaattt atcgctcgtc tgaacgaact gggcttgcag tttatgcagg
     5521 gtgcgcgctt ctggcacgta ctggatgcct ctgccggaaa agatcaggct gccaactgga
     5581 ttatcgcgac ctatcaacaa ttgtcaggca aacgcccaac cacacttggc ctgggcgatg
     5641 ggccaaacga tgcgccttta ctggaggtaa tggattacgc ggtgattgtg aaagggctaa
     5701 accgtgaagg ggtccatctg catgatgagg atccggcccg cgtctggcga acgcagcgtg
     5761 aaggaccgga aggctggcgt gaagggctgg accatttttt ccccgcccgt taagcgttat
     5821 nnnnnnnnnn gtcggccaat gactggagtt gttcaaaatc ataatcaccg gtttcctcgc
     5881 tgctacttac ccccagcgag gcactgatgc gtatcgttgt actcttggcg atcagcatct
     5941 ctttttcatt taacttcagg cgaatacgtt ctgcgacctc cgcagcctgc gtcagattcg
     6001 cgcctggcag aatcacacaa aactcctcac caccgacccg cccggcaatg tcctgcgcac
     6061 gcaaggaact gctaattaat ccggcagcat gagaaagaac acggtcgccc gcctgatgac
     6121 caaagcggtc attaatcgct ttaaaatggt caagatcgac ctgaatgaca gaaaaaggat
     6181 gttggtgcgt gtgacacaat ttagcgagcg gacaggcttt ttcgaacaat gcgccacggt
     6241 tatataaacg cgttaaggtg tcgtgccacg cctgccactg caacgagctt tgcagaacat
     6301 acatgttgct gaccatccgg cgaatcacat accaggagat gagtaacatg gtggtaaaga
     6361 gcgcccacag cagggttaat gcaatgctga tactgccgaa atcgccgcgc acgccttcgc
     6421 ttagcgtatg gacgcgcacc agcacaccgt cgaaatggtc cagacgttcc cagctaacat
     6481 agcgactgtc catacgaatg ccgccccgcg tgtcatgttc cattgcctgc gccagcaagg
     6541 ccagttcacg aggatcaaaa atattccctg ttggatggtt aggatttgaa gaggttaaaa
     6601 atctcagctt actgtaatag ggctgatact caccatcgag gtttttatcg atggcgtttc
     6661 ttaaaaattg ctgcatagta cgcacgggaa tactcatccc cagcacgcca taccagtagt
     6721 tattactgtc taccggaaca ctgacggtaa cctgcggttc agtattgctg gcgtgttccg
     6781 gttgcgaagt gaaccagcgt accgcgcggt gacgatt
//
LOCUS       DAWDRC010000155         6805 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5258_length_6805_cov_2.671704, whole genome shotgun sequence.
ACCESSION   DAWDRC010000155 DAWDRC010000000
VERSION     DAWDRC010000155.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5258_length_6805_cov_2.671704"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2954..2963
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4231..4240
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1910 a         1509 c         1605 g         1761 t
ORIGIN      
        1 aaagatgcta atgacaaccc tgtaactggc ctcaatccgg atgcaccgtc attatcgggc
       61 gcagctgctg ctggttctac ggcatcaggc tggacggata atggcgacgg gacctggact
      121 gcgcagattt ctctcggcac tacggcgggt gaattagacg ttatgccgaa gctcaatggg
      181 caggacgcgg cagcaaatgc ggcaaaagta accgtggtgg ctgatgcatt atcttcaaac
      241 cagtcgaaag tctctgtcgc agaagatcac gtaaaagccg gtgaaagcac aaccgtaacg
      301 ctggtggcga aagatgcgca tggcaacgct atcagtggtc tttcgttgtc ggcaagtttg
      361 acggggaccg cctctgaagg ggcgaccgtt tccagttgga ccgaaaaagg tgacggttcc
      421 tatgttgcta cgttaactac aggcggaaag acgggcgagc ttcgtgtcat gccgctcttc
      481 aacggccagc ctgcagccac cgaagccgcg cagctgactg ttattgccgg agagatgtca
      541 tcagcgaact ctacgcttgt tgcggacaat aaaactccaa cggttaaaac gacgacggaa
      601 ctcaccttca ccgtgaagga tgcgtacggg aatccggtca ccgggctgaa gccagatgca
      661 ccagtgttta gtggtgccgc cagcacgggg agtgagcgtc cttcagcagg aaactggaca
      721 gagaaaggta atggggtcta cgtgtcgacc ttaacgctgg gatctgccgc gggtcagttg
      781 tctgtgatgc cgcgagtgaa cggccaaaat gccgttgctc agccactggt gctgaatgtt
      841 gcaggtgacg catctaaggc tgagattcgt gatatgacag tgaaggttaa taaccaactg
      901 gctaatggac agtctgctaa ccagatcacc ctgaccgtcg tggacagcta tggtaacccg
      961 ttgcaggggc aagaagttac gctgacttta ccgcagggtg tgaccagcaa gacggggaat
     1021 acagtaacaa ccaatgcggc agggaaagtg gacattgagc ttatgtcaac ggttgcaggg
     1081 gaacttgaga tcgaggcctc ggtgaaaaac tctcagaaga cggtcaaggt gaaattcaag
     1141 gcggatttca gtaccggtca ggcgagcctg gaggtagacg ccgctgctca aaaagtggca
     1201 aacggcaaag atgcctttac gctgacggca acggttaagg atcaatacgg caaccttctt
     1261 cctggcgctg tggtcgtctt taatctgcct cggggcgtca aaccgcttgc agacggtaat
     1321 atcatggtga acgccgacaa ggagggtaaa gcggaactga aagtggtttc cgtgactgcc
     1381 ggaacctatg agatcacggc gtcagcagga aatgaccagc cttcgaatgc gcagtctgta
     1441 acgtttgtgg ctgataagac tacggcgacc atctccagta ttgaggtgat tggcaaccgt
     1501 gcagtggcgg acggcaaaac caaacagacg tataaagtta cggtgactga tgccaataac
     1561 aacctgctga aagatagcga agtgacgctg actgccagcc cggaaaattt agttctgact
     1621 cccaatggga cggcgacaac gaatgagcaa gggcaggcta ttttcaccgc cacgaccact
     1681 gtcgcagcga catatacact cacggcgaaa gtggaacagg ccgacggtca ggaatcgacg
     1741 aaaactgccg aatctaaatt cgtcgcggat gataaaaacg cggagctcgc cgcaacatct
     1801 gatgtgcatt ctctggtggc ggacgggaag actactgcaa cactgacggt tactctgatg
     1861 tcgggtgtca accccgtagg aggaaccatg tgggtcgaca ttgaggctcc ggaaggggtg
     1921 acagaggcgg attatcagtt cctgccgtcg aaaaatgacc atttcgcgag cgggaaaatc
     1981 acgcgtacat ttagtaccaa caagccaggt acatacacat tcacattcaa ctctttgaca
     2041 tatggagggt atgaaatgaa accagtgact gtgacaatta acgccgttcc tgcagatact
     2101 gaaggcgctg aggagaaata aaaacgacga tgtaataaat aataatggcg ttgacatgcg
     2161 cagcgccatt atttactgaa tacctaccct ggcaccgatt agacggtggc agggtagggt
     2221 aaatcttatg tgatggagtt acaggctaaa ccgggcgaac ggggataaaa atttcgcaaa
     2281 caatgttatt gctatcaatt tcgacgaagt attcattgta ataaaatcgc tcaatatcac
     2341 tgccgttcac tctggctaac cccctggcag gtaattccat cagatagagg tttcgcgccc
     2401 attcagggta gtgctcccac tcaccttcat agcggaaaga ggcatacagt cctcggttaa
     2461 ttgtccactc tttcggcatc tctgaatcgg tttgtttgca taccgtaccg gcaaaagttt
     2521 caactgtgag caaatcgacc gattttgttg atggttcgaa cgtggagacg aaatagatat
     2581 ccgtcttaaa attcttcata catctgacta aatgatgacg tcgcagtttg acttcttcat
     2641 ccggtaattt attcgacact tcaatatcat atttaaatga attgccaaaa ataggtgtct
     2701 caggaaagtt gactaatcta cactccgtct tttgctgttg acgtattaaa aaagaaggga
     2761 agatatttgc gagatcccaa taatcacggt ggcggtattc acggggagag cagccgaata
     2821 actttttaaa ttcgcggctg aatgactgct gtgaatcaaa atgcaaagag agtgcaatat
     2881 caagcataga tttcgcggag agccggacaa gaatggcggc acgacaaagc cttcgtttgc
     2941 gaatgtattc tccnnnnnnn nnntattctc ccaaaggcac atgcatgaaa tttcggaaca
     3001 gaagctggat gttgcgtctg ctgtagccag atttctgtgc gatatcttcg attgaaatag
     3061 ggtgctcaag attgcactca atccactcca ggagctgctg tagaatcttc tgcctgatca
     3121 tcacatactc cgttaattct ttgtcatatc gcacattttc gttatgtccg ggccctaagt
     3181 gtatcaaaaa cagcaagtcc caaggcaaaa gcactgatag cgtctgcttc attgccccaa
     3241 aaatagagag gtttcagtcc tacattatta atgaattttt tgcataagtg atatcaaaat
     3301 ccacatacta atttgaggtt acgttttaac gtagactcat tgttcatacc taatggaggg
     3361 actgatagtg gaattttccg tattaagtaa caatctgaaa atgccgatga tgggatttgg
     3421 tgtttttcag gttaccgata aagacgtgtg caaacagtca gtgttgaatg ctatccgcac
     3481 gggttatcga ctcatcgaca ctgcagcggt atacggtaat gaagatgccg tcggtgaagc
     3541 cgttcgtgaa gccatatctg aaggtttatg tacccgagaa gagttattta taacgtctaa
     3601 attgtgggtg caggatatgt tgaatcagga tattgcagca gcaggtattg aagcatcatt
     3661 aaaaaaatcg gggctagaat acttcgatct ctatttattg caccaggcca tgcgcgatta
     3721 ttttagtgcg tggcgtgcac tcgaagatgc ctatgaagag ggcaaattaa aagcgattgg
     3781 ggtttccaat ttctaccctc atgttctggc gaacttttgt gaaacggtaa gagttaaacc
     3841 gatggtcaac caggtcgagt tgcatcccta ttttgcccaa ccagaggcgc tggcaaccat
     3901 gaagtattat aacgtgcagc ctgaagcatg ggctccgtta ggtggtggac gacataaacc
     3961 ctttgaaaat aatctgcttc agagtattgc agatgcccat caaaaatcga tttctcaggt
     4021 cattctgcgt tggaatattc aacggggagt ggtcgttatt ccgaaatcaa cacatcaaca
     4081 gcgtatcgaa gaaaattttg ctatctggga tttctcactg acagagaaag aaatggcgca
     4141 aattagttcg ctagatttgg gttatgttgg ggaatcggta aaacattttt atcctgagtt
     4201 tgttcgtggt tgtcttgctg taaaaataca nnnnnnnnnn aaatgattga tattaatcac
     4261 ttattttatc tgagacgaca aaaatttttt aacaggggag tgatattgat cttcactctg
     4321 tcatatctcc ggtaatatgg cgtcaggctt catcaacaga aagggggcct gacgcctgaa
     4381 aaagtgaaca acagacagtg ttcggattat caatattcag tttttaacgt tgaactggat
     4441 tcattactgc gttgtttaag aatatcccgc gccgaatctg ccattatcat gacagcacct
     4501 gccagcatca gagtatcttt caataccaga cgaccagcac cggataaata agggaaacca
     4561 tgatgagcat cacccaatgc gggtacccat gcctccggag tggtgattaa aaatgagagt
     4621 gttaccagcg gcgtggtaaa tgccatcagc ccacccaata aacctaacca gcgattgaca
     4681 ggattagcca aaaccagcag agcaataatc acctccacga cgccaagacc gttggaaaaa
     4741 ccataggtat tattagccgt ttgccatgct cttgcttctg gtttgtattc gccttcgtga
     4801 gtcagatact gtttatagtc ttccgggtgt tcataaaaga acgacattag tggactgttt
     4861 gcgacgaatg gtgtaatgct gtctgcctcg taagggacaa actttaataa cccaatccac
     4921 ataaaaacaa tcgcaatact cagacgaatc aatgtcaggc caattttatc tccccgactt
     4981 aacaggtgca ggtatctttc cattgtaaac atccttattt atacaatact gctgttgcgg
     5041 ttgaattatt atcaattcgc atcgatgtaa ctttccagga tgaagcatta tattttctgg
     5101 ccatatcagc aatggcattc tcaagaactg tcggtgatgc tgatactgat gagacgctga
     5161 tatgtttcag aattatttta tcatctgcat tggtggaaaa tgccataacg ccagataata
     5221 gtgttgcaaa taaaacagat tttttaaaca tataaaatac catcaatgaa gtgattaaga
     5281 agttataaag taacagggaa ttaataagat tcttttctct gagacgccag aatatttgtt
     5341 ctggcgtctg attttgagtt tatttgacta atgaaaatag atcattgagt gattcgctca
     5401 tcgacggatg agtaaatatc tgatcgcgta atatgctata aggcagcccg gcatccatca
     5461 ccattttcac tatattgatc atctcgtggg agtcaacaca tagcagtgat gcccctaata
     5521 tacgttgggt tttattatca acaatcgctt ttaatacccc acgagtatca ttcatcactc
     5581 tggcacgcgg aattgcagct acaggcaatg tcaccacctg aatatcagca ccactctctc
     5641 tggcctgttc ttctgtcata ccaaccctgg acagtggcgg tgtcatgaat acggaataag
     5701 gcacattttt ccgatcatca gtactacgtc tgccttctcc cagcaattca tcacgcacaa
     5761 tgcggtaatc atccagtgaa atgtaagtaa attgcagccc gccggtaaca tctcccatcg
     5821 cccaaatatt gtttgcggtg gtatgtaatt gcttgtcaac gacaattgcc ccgcgctcgt
     5881 ttactgcgat acctgcattt tctggatgta acgaagcggt agccggttga cgaccggaag
     5941 ctattaacag tgcatccacc gccagttggg cgtgctcgct atgcacttgc acttgatttt
     6001 catggtgact gattcgctcc acatgggcat tgaggattat atcgacaccc tgatcgcgta
     6061 aaatcgtcgc gatattatca gcaatatccc gatcttcccg aggcaaaaac agcgaagctg
     6121 cctctaaaat ggttactttg ctgccaaaat tagcgaacat agaggcgaac tcaacgccaa
     6181 tatatccgcc gcccaaaata cctaaatgcc caggcaattc ttttagatta agtaatccgg
     6241 tgctgtcata tacaccaggc gtggtggtaa ttccaggaat tggcggaacc acagcttgtg
     6301 caccggtatt aataaaaatt ttctcgccat gaatctccag atttctccca ggccgatgaa
     6361 cacgcaggct atgattattg ataaactccg cctggccgtc gatcacgtcg atattgggca
     6421 tatccgcaag attatgaaaa ttcttattac gtaaaaaatt aaccacttca tttttacgct
     6481 gtatggcacg gacaaaatct gtgtgctgct gtgcgtcatg aaccaatgtt ttggttggga
     6541 tacagccgat attaatacag gtcccgccat acattgcatt tgattgttcg atgagagcca
     6601 cacgccaacc tgcttttgcc agcgtgacgg ctaatgtttt tccagccttg ccaaaaccaa
     6661 taataactgc ctggtattta ttcatgatga tttcctgtcg aaacgcgttc atttgacatc
     6721 atgactatag acgcaaaaga gcaaataaca tttcttcaca acgcttgata ttttgtctta
     6781 tcgtctggta ttttcttttt cttaa
//
LOCUS       DAWDRC010000156         6802 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5260_length_6802_cov_2.287239, whole genome shotgun sequence.
ACCESSION   DAWDRC010000156 DAWDRC010000000
VERSION     DAWDRC010000156.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5260_length_6802_cov_2.287239"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3734..3743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4861..4870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6457..6471
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1666 a         1832 c         1606 g         1663 t
ORIGIN      
        1 ctacactggt gaacaagaaa cacttccgcc tagcccgcag tggatcctcc gccagtgtca
       61 agaggtcgcc tccctgtgtg atggcgacac cttctccggc gagcagctaa acttaatgct
      121 acagcagcgt gaatggcgcg aaggtttcct cgctgaacgc atgcaggatg agatccttca
      181 ggagcaaatc ctgatagaaa ccgaaggcga acgcatcggg caaattaacg ccctttcggt
      241 cattgaattt ccgggtcatc cacgcgcttt tggcgaacct tctcgcatta gctgcgttgt
      301 gcatattggc gatggtgaat tcaccgacat cgaacgcaaa gcggagcttg gcggcaatat
      361 ccatgcgaaa gggatgatga tcatgcaagc gttcctgatg tcggaactac agcttgagca
      421 acagatcccc ttctcagcat cgctgacatt tgagcagtca tacagtgaag tggatggcga
      481 tagtgcctcg atggctgaac tctgcgccct gataagcgcc ctcgccgatg tgccggtgaa
      541 tcagagtatc gctatcacag gttcagtcga tcagttcggt cgcgcccagc cagtcggtgg
      601 tttaaatgag aaaatcgaag gcttctttgc tatttgccag caacgtgagt taacggggaa
      661 acaaggtgtc attatcccca cagctaacgt tcgccattta agtcttcaca gtgaactggt
      721 gaaagcggta gaagaagaca aattcaccat ctgggcagta gacgatgtga ctgacgcact
      781 gccgttatta ttaaatctgg tgtgggatgg cgaaggccaa acgacgctga tgcaaaccat
      841 ccaggaacgt atcgcacaag catcgcaaca ggaaggacgt caccgttttc catggccatt
      901 acgttggctg aactggttta ttccgaactg atcggacttg ttcggcgtac acgtgttagc
      961 tatcctgcgt gcttcaataa aataaggctt acagagaaca tggtagataa acgcgaatcc
     1021 tatacaaaag aagaccttct tgcctctggt cgcggtgaac tgtttggcgc taaaggcccg
     1081 caattgccag caccaaacat gctgatgatg gaccgtgtgg tcaaaatgac cgaaacgggt
     1141 ggtaacttcg acaaagggta tgttgaagca gagctggata tcaatccgga tctgtggttc
     1201 ttcggatgcc actttatcgg cgatccggtt atgccgggat gcctgggcct ggacgcaatg
     1261 tggcagctgg tagggttcta cctcggctgg ctgggcggcg aaggtaaagg tcgcgcgctg
     1321 ggcgtaggtg aagtgaaatt cacaggccag gtgctgccga cggcgaaaaa agtgacctat
     1381 cgtattcact ttaaacgcat agttaaccgt cgtctgatta tgggcctggc tgatggcgaa
     1441 gtgctggttg atggtcgttt gatctatacc gccaacgacc tgaaagtagg tctgttccag
     1501 gatacgtctg cattctgatt ttaatcagac agattatata aaggcgaaac ctccgcaatg
     1561 cggaggtttc tttttaaaga gacagaatca ggccattact accctgtccg ccatggcttc
     1621 tcgccagcct cccagccatt gtgacctttg attcagcgtc tgatagggac acatttcttt
     1681 tgagcgtccg gcgatgccgg cctgataacc acgttgatgt gcccgttcca ggcgatctcg
     1741 tttttgtctc ttcatgcctc gtttccctca tactggtttc tggtggaaaa gaaaacagtg
     1801 attactaaat atgtaatcac agttaagcaa taccgcgaat cataactgcc gtcaatgcgc
     1861 aaaattcacg ccactgtcat atttgtgagc tacacgggca tgtcggttgt acaaaaaatg
     1921 tgaagaggaa caactttttc cgcacgttag aaacaaaaaa accgcctcag gcaacatacc
     1981 ggagacggtt atatttacaa atctttatga ttaaggtaga cgctttatct cttgcgcaat
     2041 agaagcggct tcctgactcc agacactggc cagcacttta accatctcat cgtaaccatc
     2101 ctgagtttgc actccttcca gacggaacgg acgtttgatc agttgtccct ggtggttcaa
     2161 cagccactca ccactgacaa tgaccttgcc atcatagcga ccgttaaact ccgtgacggt
     2221 aacattgagc gtgtcctggg cgcttcccag aggctgggag gcaaccaccc agccgggcag
     2281 ttgcgtactc aggttggcaa ccagggtgtt gcgcaactgt tgatccaacg ggctggccca
     2341 caggttgttg ttggcaatca catacttcac atcactggtt tgataaacca caccattccc
     2401 cgccagatag tcaggaacag cgacctgctc aacccataac aggcgattac cctggctggc
     2461 ggtgctttgt gtaccgctct gcaccacagg taactggtaa tagtttttat taatttcgcc
     2521 ggagctgcat ccggccagcc acagtgccgc aatcgtcact agccactttt tcattgtttc
     2581 gccctcttcg gctctggatc ttttttgtcc ttcgcttcaa ataccagcgc gttactcttc
     2641 tcgttgagcg ttttcagcac cggttgcagt tctcgcaaca cctgatcaag tcgctgcata
     2701 tccgccacca tcttgttgta ggctgcggag ccaggctgga agccctgcat actgcgattc
     2761 aattcacgca acgttgactg catatccgtc ggtaactgct gcatcgactg gctggcgagg
     2821 atcttgttca tgctatccag cgtcgtttgc aggtttttca ttgtgcgctg actttcagaa
     2881 agcgtactgg ttgcctgttc aatcatcgga ttcaatggca gtttgttgat cttatccaac
     2941 gcttccatca gtcgttgctg gatttgtgcc agaccgccgc taacggtcgg gataatctga
     3001 taaccattaa attcacgaat accggttatt gcaggcgtat ttggatagaa atcgagatca
     3061 acatacagtg cgccagtgac caggtttccg gttttcagcg atccgcgtaa accacgtttc
     3121 aacaattcgc caaggtgctc aacaacatcc gcattttcgc caagctgcat tttcagccgc
     3181 tctggctcga tacgaatcag taccggaata cggtaatcat cgttaaatgt ctgacgcata
     3241 ttcggcgcaa agaatggcac tttgcttacg gtacccaggc gaataccccg gaattctacc
     3301 ggagctcccg gttgcagacc gcgtaccgaa tctttaaaga acatcagata atcaatgtga
     3361 tcggtgtaca acgaatcctg aatgctcttc tgatcatcat acaaaacgaa agctgttttc
     3421 ggtgccactg gctgccctaa atccagacct tccggcacat caaagctgac accgccacta
     3481 agcagcgttg tcaatgagcc catctccaca cgcattcccg ctgacgtcag atcaaccgca
     3541 atgccactat ctttccagaa acgaacattg ctggtcacca gtcggtcata aggtgcattg
     3601 atgaacagtt gataactgat attgcgtttt tgcgtatcga aagtgctggt ttcaaccgaa
     3661 cctacccgat agccacggaa cagcaccgga tctcctggcg agagctgccc ggcttttttg
     3721 ctatcgagaa gcannnnnnn nnncaggtgg ggccaacggt ggcgagtcca gcaaatcgta
     3781 tttatccatt ttgctgcctt tcgcgcctgg ctgcagttcg atataaaccc cagacagcag
     3841 tgttcccagg ccgctaatcc cttctcgacc aatctgcggt ttcaccaccc aaaagacggt
     3901 atctttatgc agcaactttt ccataccgga attcagccgc gctttgattt caacgtgcgt
     3961 caaatcatca gccagtgtgg cgctttcaac cacgccgacg tcaacgctac ggcttttaat
     4021 ggtggttttg ccaccttcaa ttccttccgc attcgcggtg atcagggtca cttccggtcc
     4081 ctgatggctg taatgataaa aaagaaccca ggccccaatg agcgccgtga cgataggaaa
     4141 tatccacacg ggagaccagt tcttcacttt ctggattttg gcttccccat tattagattc
     4201 catactgtca ggactcctca tgctctgatt caggttgacg atcccacgac aaacgcgggt
     4261 caaacgtcat agcagaaaac attgtcatta tgacgactaa agcaaacatt aatgcaccca
     4321 ttgccggata aatactcatt aaacctccca tacgcaccag cgccgagagc accgcgataa
     4381 cgaaaacgtc aatcatcgac cagcggccta caaactcaac aacttcataa atcaaatgca
     4441 ttctttcact atcgcgcttg ccgtgtcctt tggcatccca acacagccac gcaatggcga
     4501 tcatctttaa cgttggcacc ataatactgg ccagaaaaat caccgcagcg acgggataag
     4561 agccttcgct ccataacaga atgaccccag cgagaatcgt cgacggcatc ttcgagccta
     4621 ataaatccgt caccatgatg ggcaaaatat tagccggaag atacagcatg atggacgtta
     4681 caagcagcgc gagtgtccac tgcaggctgt ttctacgccg aacgtaccct ttggtactac
     4741 aacgcgggca cacgggttca tcagcaggaa ggattgccgt acaacaggag cacgaacgca
     4801 gcccctgacg tatccccgtg acacctggtt ttagcggctg gcgcagttct ggcatcgggg
     4861 nnnnnnnnnn tgtcgtccca taaccagcga cgatcaacac actgaaaagc gcgcagttgc
     4921 aggacacaaa ataaacacca ggggagaaag ctgctgccta ccccaatgct gccgtaagcc
     4981 atcagtttca cgaaactgac cagcacaccc gcgaggaaaa tctccgccat tccccaggtt
     5041 ttgagttgaa aaagcacccg tgccagttgc tcttttaaac ggaccggtaa ttccgcgcga
     5101 ttcaccagta acagaatggt tatcagacaa aacgcgggaa ccagttgcac aaacaacagg
     5161 aaaaaggtgc cgaggctggc gtagtcctca gaaaaaagca cgccgggaat ttccagtaat
     5221 gtaatttcac tggtaactcc tgcaacgttc atattcacaa aaggaaacaa gttggacagc
     5281 aacagcatga acagtgcagc caacgcatag gcggtgggac gctgccgagg ggcatcccac
     5341 gccacggtta acgttgtgcc acaccgggga catgccgctt tctgaccatg ctcaaggcgc
     5401 ggtaacgcca ccagcatgtc acactgcgag cacaggatgt gcttcgcggc atgatgatgt
     5461 tcgcacattg gtactcctta atgatgcgct attttacaga tgacgaacaa agtgttggtt
     5521 ttcatgcctg ataaacccag ttcatcaggc aattttaccg ttgtcagctg tctctgttta
     5581 aatcgactat tttgcgatca gccaccattt tttaacgctt caagatactc ccagcgttca
     5641 aaggcttgct ccagctcctg ctctgcagca gccatatcgg cgagcacttt ttgtgtctgc
     5701 tcatggggct gactgaagaa ggaagcatcc gccacttgcg tctgtagggc ttccagcttc
     5761 gcctccagat cttcgagcaa ttgcggtagc tgctccagtt cgcgctgcaa tttatagctt
     5821 agtttgctac tgctgcgttt tacagtttct gcttttggcg cggcggcttc ttcgattttt
     5881 ttcaccgcag gctgtttgag cgccacatac tgttcttgct gaccacgggc atcatgataa
     5941 ccgccgacat aacgaccaat tttaccgccg ccttcgaaga tccagcattc tgtaacggtg
     6001 ttatcgacaa actgacgatc gtggctaacc agcaataccg tgccctgata gctgtcgatc
     6061 agttcttcca gcagttccag cgtttcgaca tcaagatcgt tggtcggttc gtcaagaatc
     6121 aataagttgc ttggtttgag gaacaaacgc gccaacaaca agcggttacg ctcaccgcca
     6181 gaaagcgcac gtaccggcgt catcgcccgt tttggatgga acaggaagtc ctgcaaatag
     6241 cccaaaacat gacgtggctt gccgttaacc atcacctctt gcttaccttc ggcaaggtta
     6301 tccatcaccg ttttatcggg atccagttcc gcacggtgct gatcgaaata agccacttcc
     6361 agtttggtgc caacgtgaat acgcccgctg tccgcctgaa gctgaccgag cattagtttc
     6421 agcagcgtgg ttttgccgca cccattcgga ccaatcnnnn nnnnnnnnnn ntttgtcgcc
     6481 gcgtagaacc tgggcagaaa aatctttcac cagttgctta ccgtcaacct ggtagcaaac
     6541 gtcctccatt tcgaaaacga tcttaccgga gcggctggct tcttccacct gcatctttgc
     6601 ggtacccatt acttcgcgac gttcaccacg ctcgcgacgc attgctttca gggcgcgtac
     6661 gcggccttca ttacgggtac gacgtgcttt gatcccctgg cggatccaca cctcttcctg
     6721 cgccagtttg cgatcgaact cggcattttg taattcttcc acgcgcaggg cttcttcttt
     6781 ttccagcagg tactggtcgt aa
//
LOCUS       DAWDRC010000157         6782 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5276_length_6782_cov_2.572618, whole genome shotgun sequence.
ACCESSION   DAWDRC010000157 DAWDRC010000000
VERSION     DAWDRC010000157.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5276_length_6782_cov_2.572618"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    256..265
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1283..1312
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1718 a         1719 c         1810 g         1495 t
ORIGIN      
        1 cctcgcgctg cgtcacgaac tggcgcaact gctgggcttt gaaaactacg ccttcaaatc
       61 acttgccact aaaatggcag aaaacccgca gcaggtgctg gatttcttaa ccgatctggc
      121 aaaacgcgcg cgcccgcaag gcgaaaaaga gctggcacaa ctgcgcgcct tcgccaaagc
      181 cgaatttggc gtcgatgagt tacaaccgtg ggatatcgct tactacagcg aaaaacagaa
      241 acagcacctc tacagnnnnn nnnnngatga acaactacgt ccgtacttcc cggaaaacaa
      301 agcggttaac ggcctgtttg aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg
      361 taaagatgtt gatgtctggc atccggatgt acgtttcttc gagctgtatg acgagaacga
      421 cgaactgcgc ggcagcttct acctcgacct gtatgcccgt gaaaacaaac gcggcggggc
      481 gtggatggat gactgcgtag gccagatgcg taaagccgac ggttcgctgc aaaaaccggt
      541 cgcgtatctg acctgcaact tcaaccgccc ggtaaatggt aaaccggcgc tgtttaccca
      601 tgacgaagtg atcaccctgt tccacgagtt cggtcacggc ctgcaccata tgctgacccg
      661 cattgaaacc gctggtgttt ccgggatcag tggggtgccg tgggatgcgg tcgaactgcc
      721 gagccagttt atggaaaact ggtgctggga gccggaggcg ctggcgttta tctccggtca
      781 ctatgaaacc ggcgagccgc tgccgaagga gctgctggat aaaatgctgg cagcgaaaaa
      841 ttaccaggcg gcgctgttta ttctgcgcca gctggagttc ggcctgttcg atttccgcct
      901 ccatgccgag ttccgtccgg atcagggggc gaaaatcctc gaaactctgg cagaaatcaa
      961 gaaactggtt gccgtggtac cgtctccgtc atggggccgt ttcccgcatg ctttcagcca
     1021 catttttgcc ggtggttatg cggcaggtta ctacagctac ctgtgggccg atgtgctggc
     1081 ggcagatgcc ttctcgcgtt ttgaggaaga gggcattttc aaccgtgaaa ctgggcaatc
     1141 gttcctcgac aacattctga gccgtggcgg ttcagaagag ccgatggagc tgttcaaacg
     1201 cttccgtggt cgtgaaccac agctggatgc gatgctggag cattacggca ttaagggctg
     1261 aacattcagt gaaaatctgc ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaattgatg
     1321 aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg ctggaacacg
     1381 atgaagacaa cctgatggcg ctggtgttaa cgccggagca tctggaactg cgcaagcgtg
     1441 atgagccgaa acttggcggc atctttgttg attttgttgg cggagcgatg gcgcaccgac
     1501 gcaaattcgg cggtggtcgc ggagaggcgg tggcgaaagc ggtgggcatt aaaggcgatt
     1561 atttgccgga tgtggtggac gccaccgcag gattagggcg cgatgccttt gtgctggctt
     1621 cggtcggctg ccgcgtgcgg atgctggagc gtaatccggt ggttgccgcg ttgctcgacg
     1681 atggtctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttacag gaacgtttgc
     1741 agttgattca cgcctccagt ctgacggcgc tgagcgatat caccccgcgt ccgcaggtag
     1801 tttatcttga cccaatgttc ccgcataagc agaaaagcgc gctggtgaaa aaagagatgc
     1861 gcgtgtttca gtcgctggtg gggccggatc ttgatgccga tggattgttg gagcctgcgc
     1921 ggttgctggc aaccaaacgc gtggtggtga agcgtccgga ctatgcgccg ccgctggcga
     1981 atgtcgccac gccaaacgcg gtagtcacta aagggcatcg ctttgatatt tatgccggaa
     2041 cgccggtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg caagaataat
     2101 taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa tagcatcact
     2161 acggcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata gacgggcaac
     2221 gtctcaagtg gatcggtaat gttgtctggt actgcggtaa atgttgccac atagccgccc
     2281 agcaagaacg cggcagcctg cgtcaggaac cacatcccga gaataaagcc catcaaatgc
     2341 tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacag ttcacctaag
     2401 ctctggaata agtacaccag cacgataaac catggcgatg tcagcccctg ggcatccgca
     2461 aaccacattc cagccgctgc agccgtcaaa aagcccattg agcacataaa catgccgaga
     2521 gtaaacttca tcggcatcga gagatctttg cctttgttac ccagatgcgt gtaaatgcct
     2581 gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa gctgaccggg
     2641 ttgatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa gttcagcgac
     2701 gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag gacaaaggcg
     2761 acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa gaagatgatg
     2821 gtgacgacga tggagagaac aatcagaacc agattggcga cttctacgtt gtgcatcagc
     2881 caggcgcaga cgaagatcat caccacgctg ccaagtaata cgtacaacag tttactgaag
     2941 ctcatcggcc ggaagtcggg ttcagaacca atgtctttca ccattccacg acaggcgatg
     3001 taaaccagta atgcgataat taaccctgca ccgcacaggt tgtaggtgac tgaatagccg
     3061 aatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat attgatcgac
     3121 atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcgggta gcacttcgaa
     3181 agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt ccccagggcg
     3241 atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc aatcgccagc
     3301 acaagtgctc cgagaacaat ggtgcgtttg gttcccagca ggtggtcgcc aacatagcca
     3361 ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac aaaagcctgc
     3421 tcttgcgaga acccaagctg tttaacgaag aaaaccgcca gtacgccctg cacgccgtag
     3481 tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg aggttgctgc
     3541 agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc atcctgaaaa
     3601 gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga ttcttaatat
     3661 tgatgcggcg aatagattaa tacactatta caacagaaaa taaccagctg atcacactaa
     3721 tgatagaaaa tatgatggtg ttaaatgttg tattggcagg ggttttgatt gcgtacgcag
     3781 accgcaggcc agataaggcg tttacgctgc atcaggtaac aaggcgcgcc aaaaagcaaa
     3841 acgcccgcat atgcgggcgt tcaggaacac gtcgtggagg gaagattatt cttcttcgtc
     3901 gcgcagcgga acaatcagca tatcaacgtg aacggtgttg atcagctgac gtgcggaaga
     3961 catcagtttg ctccagaagt cctggtggtg accacaaacc accagatcca tatcgtattt
     4021 cttgattgca tcgaccagta cctggcccag atcgccgctg ccgctcaggg tttcagtgat
     4081 tgggtagcct gcattggttg aaagctcggt cagcgcatga tgtgtctctt cagagatgcg
     4141 tttctgcata tcacccagat tcacatcgat aagcccggtg tataggtcag agtagtttac
     4201 atctacgtgg atcagagaaa ctttcgcatt gtaggggcga gccatagaga ctgctttctc
     4261 taccagaact ttgctttccg gggagaggtc gaccgcgatg agaatgtgtt tataagccat
     4321 agtgttactc cttccataaa gttgtcgatg actggccagc tagcgtttct tgtgccgtta
     4381 cgtcacccgc gtcctgcgaa ctacgtgcgc ggggctaacc cagccgtaaa gattcagtgt
     4441 taagaccatc cttaccttat agcgacccgg atgatccgtc aatccgcctt gcttactcgt
     4501 tagataaaca aattttcgac atgaatatat tgatagtggt taaccttctg gaaaaaaaac
     4561 aacctgatct cctacactat ctatagagcc gctcggatgt tggtcaccac aaaagcggtt
     4621 tctccaggac ctggtgttaa ctgattggca gcgtatcggg agcggtagcc ccggggtgga
     4681 actccgcggg caggtcgccg gggaggaggt atgataagca ccgtcgcatt attttgggct
     4741 ttatgtgtcg tttgcattgt taacatggcg cgctatttct catcactacg cgcgttgtta
     4801 gtggtactgc gtaactgcga tccattgctc tatcaatatg ttgatggagg gggctttttt
     4861 acctcacatg gccaacccaa caaacaggtg cgtctcgttt ggtatatcta tgcccaacgt
     4921 tatcgcgatc atcacgatga tgagtttatt cgccgctgtg agcgggtgcg tcggcagttt
     4981 attctgacca gcgcattgtg tggtctggtg gtggtcagcc tgattgcatt gatgatttgg
     5041 cattaagcac ttcagcaata atgacgcata aaaaaagcgg gccagtaaac tgacccgctc
     5101 gttttggtgc gtacgattac aggaactgca aggagagcca gtacagcccg ccggaaagca
     5161 gtaccgcagc cggaagggta aacacccagg ccatcagaat actggttacg gttttacgct
     5221 gtaagccgcc gccatctacc accatcgtcc ccgcgacaga agaggagagt acgtgagtag
     5281 tggaaaccgg catcccggta taacttgcca ggccgataga cactgccgcc gtcatctggg
     5341 cagacatccc ctgagcgtag gtcatgcctt tcttaccgat tttctcaccg atagtcgttg
     5401 ccacacgacg ccagccaatc atcgtaccga tacctaacgc cagcgcgacc gccatgatga
     5461 tccacaccgg cgcatactcg atggtgctaa gcatgtcgga cttcagtttc ttcaacaggc
     5521 gctgatcgtc agcactcacg ccaggcatct tcaccacttt gtcgatagtg tcagaaacgc
     5581 acagcataat gcggcgcatc tggctacgtt gatcaagcga cagcttgtcg tagctttcca
     5641 catcggtagt caacatgcct ttcaggcggt tgagcgcgtt aatggtattc gacggatggc
     5701 aatggaactc cgcgggttgc gttgcgccag cttccggagc cggtactaac tgatcagcac
     5761 cggtcgcttg tttcagcagc gcaggatgct gctcaaagta agcttcgacg ttgttgatgg
     5821 catcacgggt acgggtgatt tcgtagccag tggcattcat gttcaccacg aagcctgctg
     5881 gtgcgacacc aatcaatacc aacataacca gaccaatgcc tttctgacca tcgttcgcgc
     5941 cgtgcgaaaa cgccacgccg atagcggaaa ggatcagcgc aatacgcgtc cagaacggcg
     6001 gctttttctt gccgtctttc ttttcacgct ccgctggggt caggtggata cgggcgcgtt
     6061 tcttggtgcc gctccagtag cgacgcagca agaaaatcag accgccagca aacaccaggc
     6121 cgacaatagg ggaaacgatc agagaaccga aaatacttaa tactttcggg atattgagtg
     6181 catccaccac tgacgtcccg gtcatcaacg cattggttaa accaatcccg atgatcgcgc
     6241 caatcagcgt atgagagctg gatgcaggta aaccaaagta ccaggtaccc aggttccaga
     6301 taatcgccgc cagcaacata gagaacacca tggcaaggcc atgagacgat cccatattaa
     6361 gcagcagatc cgtcggcagc atatgcacaa tggcataggc aacactcaga ccacccagca
     6421 aaacacccaa aaagttgaat accgccgcca taaccacggc gagctgagaa cgcatcgcgc
     6481 gggtatagat aacggttgcc acggcgttgg ctgtgtcatg gaaaccattg atggcttcgt
     6541 agaacagcac aaaagccagt gcaagcaata ataacagccc ggtatgcaaa tccaggccag
     6601 caaacaaatg tagcatagga cgttacgcca ttttgaggac atgaacgcgg cgcattatca
     6661 gtgactttcg cggcgcgggc aaagtgaaat atagactttt tttgatttgc ctcctgtatg
     6721 gattacactc aaaaaataat tatcttatat aattcaggca aatacttcct tttagtaata
     6781 tt
//
LOCUS       DAWDRC010000158         6777 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5280_length_6777_cov_2.264653, whole genome shotgun sequence.
ACCESSION   DAWDRC010000158 DAWDRC010000000
VERSION     DAWDRC010000158.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5280_length_6777_cov_2.264653"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5015..5024
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6014..6023
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1665 a         1892 c         1694 g         1506 t
ORIGIN      
        1 agcacaatgc tatgcgcact gtcacccgct caacagatca cggcaatcgt tcgtttttta
       61 tactgctcag ggataaaaaa aggcccctgt tgaaattgca ggggcctggt acgagcaagc
      121 atcatattgg gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat
      181 tcctttcatt tcagcaaccg ccgccccgtc tacgacataa cgcgaatact cgtccgcgtg
      241 tgcatctgat gacatggaca atccttgatt acgataacgc atcggtgagg cttgccacgc
      301 tcccgcggag ctatggactt ccagcactcc ggcatcgagg aagtggtgca ggttttctgc
      361 acggactcct gctccggcca taatgattgg agcatcacga tgggcaataa gttccataat
      421 ttttgataaa ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc
      481 caattccgta agattattga gtgtatttaa agggttagcg cacatatcga aggcgcgatg
      541 aaaagtcact gccagcggac cggcagcagc cattattttt tccattcgtg gcatatcgac
      601 attcccgtca acatcgagaa cgcccgtcac cagtccagga aaacctaatt cgcggactgt
      661 gcgcacatcc tcaagaatgg cggcaaattc accgtcgctg tagcaaaaat caccaccgcg
      721 tgggcgaatt atcggatgca cagggatcgt caaccgctgg cgcacggatt tcagtacacc
      781 caacgacggc gttaagcccc cctcttttgg agctgcgcat aattcaactc tgtcagcgcc
      841 gttttgctgc gccgttagcg cgcattccat gctgtaacag caaatttcca gtaatgccat
      901 ttttgctcct tagttacgcc gactgctcgc tggcaacgat ctcttcaatg gaccacggat
      961 gaaacttaat ggttgtttta ccatcggtga ccgccagcgt cggattcggt aatcgttcat
     1021 gctcaccttt tggcgaactg gttttaactg agatccccgg caggctgagt ccttcatcag
     1081 aaagcaatgc cagcgcacgg gcattcagcg tttctggatc acccgacaga acgatttcaa
     1141 tatgttccca accttcgtgc gggtaacgtt tttccccggg ccacggtagc tccacaatgg
     1201 aaaactgcca gtgcgcaacc tgtaccggtt catgcaattt aaacaaacaa atcggtctgc
     1261 cattgatcat attttctgac aaaagctcgc cgcactgttc aaacccgcga cgccagcgtt
     1321 cagcggtgac gttttgatgg caacgcaagg aaatgtgatc ggcagtcagc ggagtgatat
     1381 ccaaccccag acggcgggaa agttcatcta atgcgtgggt aaatcgcggt aaatccgatg
     1441 caatatcctg cagctcgtcg atagattgcc agttcgccat aatcactctt cgtctttcat
     1501 taaaagcgtt aatttaccct gttgccctgc gccaaccaac cgctgatttc acgccgcttc
     1561 tgatgcaata gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa acagccattt
     1621 gcagtatact cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca
     1681 atgtaaggta ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg
     1741 attgcggcag gcgcgcctgc ggattgcgaa ccgcaggttc gtcagtcagc aaaagttcag
     1801 ttcggcgact atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg
     1861 cgacaattag cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt
     1921 gagatcgccg gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat
     1981 gttcagcagg cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt
     2041 gtggttgact actctgcgcc aaacgtggcg aaagagatgc atgtcggtca cctgcgctct
     2101 accattattg gtgacgcagc agttcgtact ctggagttcc tcggtcacaa agtgattcgc
     2161 gcaaaccacg tcggcgactg gggcactcag ttcggtatgc tgattgcatg gctggaaaag
     2221 cagcagcagg aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat
     2281 gcgaaaaagc attacgacga ggatgaagag tttgccgagc gtgcacgtaa ctacgtggta
     2341 aaactgcaaa gcggtgacga atatttccgc gagatgtggc gcaaactggt cgacatcacc
     2401 atgacgcaga accagatcac ctacgatcgt ctgaacgtga cgttgacccg cgatgacgtc
     2461 atgggtgaaa gtctctacaa cccaatgctg ccgggtatcg tggcggacct gaaagccaaa
     2521 ggtctggcag tagaaagtga aggcgcgacc gttgtattcc ttgatgagtt caaaaacaag
     2581 gaaggcgaac cgatgggcgt tatcattcag aagaaagatg gcggctatct ctacaccacc
     2641 actgatatcg cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat
     2701 tacatcgatt cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagcg
     2761 ggctatgtac cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggcaaa
     2821 gatggtaaac cgttcaaaac ccgcgctggc ggtacggtga aactggccga tctgctggat
     2881 gaagccctgg agcgtgcacg tcgtctggtg gcagaaaaga acccggatat gccagccgac
     2941 gagctggaaa aactggctaa cgcggttggt attggtgcgg tgaaatatgc ggatctctcc
     3001 aaaaaccgca ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat
     3061 accgcgccat acatgcagta tgcttacacg cgtgtattgt ccgtgttccg taaagcagaa
     3121 attaacgaag agcaactggc tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa
     3181 ctggcagctc gcctgctgca gtttgaagaa accctcactg tggttgcccg tgaaggcacg
     3241 ccgcatgtga tgtgtgctta cctgtacgat ctggccggtc tgttctctgg cttctatgag
     3301 cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca
     3361 caactgacgg cgaagacgct gaagctgggt ctggatacgc tgggtattga gacagtagag
     3421 cgtatgtaat cgcgttatac ggcaacagcc gatgtcattg cagacatcgg cttttttatc
     3481 attaacgata atttacaatc acttcattcc gctgaatctt tagcggcgga attaaccgcc
     3541 caccgccagg cacttcccag ataaagcgca acggttctgc cgccggtata ccggaaaagg
     3601 ccatggtggt tccactctgc ccctccaatt cgacacaacg agattgtgaa cacaagcgaa
     3661 cccgcagtcc tgcaggtgtc ggaccgataa gctgataacg ccacgccacc agcgtcatca
     3721 atcctgaagc aggttgtcgc gtagaaagag gcgctgacga catcgattca ccgcgatgat
     3781 ttagcgtaac gcccacgcta ctcgcctgcc acatcccctc cccggcggct tgcaccagca
     3841 gcggaaacaa taatattgct aataaggctc tcattatttg ccgccaatcg tcgccgtcat
     3901 acggatatgt cggttatctg atagttccag attcgacagc accactaact gcggcaagct
     3961 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca ataccggcgg
     4021 cgcacccaac atctcctgac gggatagcgc ttcctgagtt tgcgccagta aacgatccgc
     4081 cagccctggc tccagtcctc ccccgccttg caaagcctgt aacagtaaac gctccagtgg
     4141 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
     4201 ccgtcccaac gctacgcgca cgacggcggt taattcatgc ggatcgctct ggatgggcgc
     4261 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggcactt tttcatcgag
     4321 cagattttgc agcactttat gcagtgtggt aagcgtgacg acgccaggaa cgagatcttc
     4381 cgtcagcttt ggcatctcct gggcgacgcg atccagcagc tgttgcgcct cctgacgacc
     4441 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcca ccaccgtgct
     4501 ggcctcaacc acggtgtacc cctgaatctg cgcctgttct ttaagcgcac tttcaatcca
     4561 gatagcattc aggccaaatg ccggatcaac ggtcgcttca ccaggtaatg ttccggcggc
     4621 agtgcctgga ttaattgcca gccagcgccc cggataagca tcaccactcc ccatttcgac
     4681 acctttcatc aagatacgat agcgggctgg ttgcaaatcc atgttatcgc ggatgtgcac
     4741 cactgggggt aggaagccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     4801 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     4861 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     4921 tgccattttt accggttttg cttcggcagg cgctttttgt tcacgtccgc ggatccacca
     4981 ggccagtccc agcaatccgg cagtgaacag caaannnnnn nnnngcaaaa ataccaggtt
     5041 cggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca acataacgct
     5101 cggattactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg
     5161 tgtaacgatg acgcccgcgg cggtagaaat aaccagtgcc gggatttgtg ccaccagacc
     5221 gtcgccaatg gtcaatagcg tataactttc cgccgcgtgc cccatgctca tgccatgttg
     5281 cagcacaccg accagcaatc cgccgacaac gttaatgacc atgatgagga tcccggcgat
     5341 ggcatcgccg cgaacaaact tacttgcccc gtccatcgag ccgtaaaaat cggcttcctg
     5401 agtcacttcg gagcggcgtt ttttcgcttc atcttcacca atcaatccgg cgttaaggtc
     5461 ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg cgcccacttc
     5521 cgcaatacgc ccggcacctt tggtgataac cataaagttg atgatcacga gaatgacgaa
     5581 caccacgata ccgatagcga aattgccacc aacgaggaag tgaccgaacg cttcgaccac
     5641 ctttccagcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg ttgaagccac
     5701 gttaagtgcc agacgcaaca gcgtggtaaa caacaggatg gtcggaaacg cagcaaactc
     5761 gagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg caatattgaa
     5821 ggtaaacagc agatcgagta tgaatgcggg cagtggcagt accatcatcg acaagatcaa
     5881 caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt ttgcaggcag
     5941 gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa tccagggctt
     6001 ccggcaccgg aagnnnnnnn nnncgatctc cgcatgtcga tacagcgcac gcgccagcgg
     6061 cggcgcttca agcgtcggga cgttattttc agcgccaatt tcacgaatgc gcagcgcgac
     6121 cagccctgca cctttagcga ccactttcgg tgcgctcatt ttgttttcgt catactgcaa
     6181 cgctaccgaa tagtgggtcg ggttattgac aatgacatcc gctttcggca catcggccat
     6241 catccgacgc cgtgcggccg ctcgctgcat ctgacggatc cgccctttaa catgggggtc
     6301 gccttcgctt tgtttgaact catcacgaat atcctgccgt gacattcgca gctttttcag
     6361 gtggctgaag atttggaaaa agacgtcaaa tcccaccatt ggaatgacac caagcaccac
     6421 cagcagcgcg catagcccta ccagatccat cgcattaccc atggcggtaa tcggagactc
     6481 ggccattaag cgcatcatct gcggccagtg ataccagaga aaaaaccccg tcacgctgcc
     6541 aaccaggatg gttttcaaaa ttgccttaag caactccgcg ccagtctgtg ccgagaacat
     6601 ccgtttaatg cccggtagcg ggttgagttt ggaaaacttc ggctgcaagg attttccgct
     6661 aaataccagc ccccccagca taaccggaga aataatcgcc accagcacca cgccgctaat
     6721 cagcggtagc agcgccagca tggcttctct gatcagcaga ataatctgcc cgaggat
//
LOCUS       DAWDRC010000159         6741 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5320_length_6741_cov_2.580018, whole genome shotgun sequence.
ACCESSION   DAWDRC010000159 DAWDRC010000000
VERSION     DAWDRC010000159.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5320_length_6741_cov_2.580018"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1436..1445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1678 a         1727 c         1705 g         1621 t
ORIGIN      
        1 ttaagcgacg cgcgacgctt tgtttcggca gcccgtagtg cctcgcgcaa taaaccgatt
       61 ttggtgatta aaagcggacg tagcccggcg gctcaacgtc tgttgaacac cacggcagga
      121 atggatccgg cgtgggatgc agctattcag cgtgccggtt tgttgcgggt acaggacacc
      181 cacgaactgt tttcggcggt ggaaaccctt agccatatgc gcccgctgcg tggcgaccgg
      241 ttgatgatta tcagcaacgg tgctgcgcct gccgcgctgg cgctggatgc cttatggtca
      301 cgcaatggca agctggcaac gctaagcgaa gaaacctgcc agaaactgcg cgatgcactg
      361 ccagggcatg tggcaatctc taacccgctc gatctgcgcg atgacgccag cagcgagcat
      421 tatgtcaaaa cgctggacat tctgctccac agccaggatt ttgatgcgct gatggttatt
      481 cattcgccca gcgccgctgc tcccgcaaca gaaagcgcgc aagtattaat tgaagcggta
      541 aagcatcatc cccgcagcaa atatgtctct ttgctgacga actggtgcgg cgagcactcc
      601 tcgcaagagg cacgacgttt attcagcgaa gccgggctgc cgacctaccg tactccggaa
      661 ggaaccatca ctgcttttat gcatatggtc gagtaccggc gtaatcagaa gcaactacgc
      721 gaaacgccgg cgttgcccag caatctgact tccaataccg cagaagcgca tcttctgttg
      781 caacatgcga ttgccgaagg ggctacgtcg ctcgataccc atgaagttca gcccatcctg
      841 caagcgtatg gtatgaatac tctccctacc tggattgcca atgatagcac cgaagcggta
      901 catattgcca aacagattgg ctatccggtg gcactgaaat tgcgctcgcc ggatattcca
      961 cataaatcgg aagttcaggg cgtcatgctc tacctacgta cagccaatga agtccagcaa
     1021 gcggcaaacg ctattttcga tcgcgtaaaa atggcctggc cgcaggcgcg ggtccacggc
     1081 ctgttggtgc aaagtatggc taaccgtgct ggcgctcagg agttgcgggt tgtggttgag
     1141 cacgatccgg ttttcgggcc gttaatcatg ctgggtgaag gcggtgtgga gtggcgtcct
     1201 gaagatcaag ccgtcgtcgc actgccgccg ctgaacatga acctggcccg ctatctggtt
     1261 attcagggga tcaaaagtaa aaagattcgt gcacgcagtg cgctacgccc attggatgtt
     1321 gcaggcttga gccagcttct ggtgcaggtt tccaacttga ttgtcgattg cccggaaatt
     1381 cagcgtctgg atattcatcc tttgctggct tctgtcagtg aatttaccgc gctggnnnnn
     1441 nnnnnctgga tatcgcgccg tttgaaggtg ataacgagag ccggctggct gtgcgccctt
     1501 atccgcatca gctggaagaa tgggtagaat tgaaaaacgg tgaacgctgc ttgttccgcc
     1561 cgattttgcc agaagatgag ccacagcttc agcaattcat ttcgcgagtc accaaagaag
     1621 atctttatta ccgctacttt agcgagatca acgaatttac ccatgaagat ttagccaaca
     1681 tgacgcagat cgactacgat cgggaaatgg catttgtagc ggtacgacga attgatcaaa
     1741 cggaagagat cctcggcgtc acacgtgcga tctccgaccc tgataacatc gatgccgaat
     1801 ttgccgtgct ggttcgctcg gatctcaaag ggttaggctt aggtcgacgc ttaatggaaa
     1861 agttgattac ctatacgcga gatcacggac tacaacgtct gaatggtatt acgatgccaa
     1921 acaatcgtgg catggtggcg ctggcccgca agctcgggtt taacgttgat atccagctcg
     1981 aagaggggat cgttgggctt acgctaagtc ttgcccagcg cgaggaatca tgagtaaggt
     2041 actggaaatg ttgaccactt aatcgggact ggtgttatta ttgcccgctt atgtcgtctg
     2101 cattgcacag aggacccttc aatgaacaga gaagaaatgc actgtgatgt tgtcaaaatt
     2161 taagcgtaat aaacatcaac aacaccttgc ccaactaccc aagatttctc aatcagttga
     2221 tgatgtcgat ttcttttacg ctcccgccga cttccgggag acgttgctgg aaaaaatagc
     2281 cagcgcgaag cagcgcattt gcattgttgc cctgtatctc gaacaggatg acggtggcaa
     2341 aggcattctg aacgcgttgt atgaggctaa aaggcagcgt ccggaactgg atgtgcgggt
     2401 gctggtcgac tggcatcgtg cacaacgtgg acgcattggc gctgcggcat ctaacactaa
     2461 cgctgactgg tactgccgca tggcgcagga aaatccgggc gtagatgttc cggtttatgg
     2521 cgttccaatc aatacccgtg aagcccttgg tgttctgcac tttaaaggct ttatcatcga
     2581 cgatagcgta ctttatagcg gtgccagcct gaacgatgtt tacctgcatc agcacgataa
     2641 atatcgctac gaccgttatc atctgatccg taaccgtaag atgtcagaca ttatgtttga
     2701 atgggttaca cagaatatta tgaatggccg cggcgttaat cgtctggatg atgttaatcg
     2761 gccaaaaagc ccggaaatca agaacgacat tcgcctgttc cgccaggagc tgcgtgatgc
     2821 cgcttatcat ttccagggcg atgccgacaa cgatcagctt tccgtaacgc cgctagtggg
     2881 gctggggaaa tcgagtctgt tgaacaagac cattttccat cttatgcctt gtgccgagca
     2941 gaaactaacc atctgtacgc catacttcaa cctgccagca atccttgtgc gcaatattat
     3001 ccagttgctg cgcgaaggga aaaaggtcga aattattgtt ggtgataaaa ccgcgaacga
     3061 cttctacatt ccggaagatg aacctttcaa gataattggc gcattgcctt atctctatga
     3121 gatcaatctg cgtcgtttcc tgagtcgttt acagtattac gtcaacactg accagttagt
     3181 agttcggtta tggaaagatg acgacaacac ctatcacctg aaagggatgt gggttgatga
     3241 taagtggatg ttgatcaccg gtaataacct gaacccgcgc gcctggcgtc tggatctgga
     3301 aaacgccatt ttgatccacg atccacaact ggagcttgcg ccgcagcgag agaaagaact
     3361 ggagctgatc cgcgagcata ccaccatcgt taagcactat cgcgatctgc aaagtattgc
     3421 cgattatccg gtgaaggttc gtaaactcat ccgccgtctg cgccgtatcc gcatcgaccg
     3481 attaattagc cgcatcctgt aatcacaacc ccgtcctgta cggggtttgt tttttggagg
     3541 ccacgttttg cgtattcttt ttgtctgttc gctattgtta ctttctggat gcagccatat
     3601 ggctaacgat aactggagcg ggcaggataa agctcaacac tttatcgcct cggcgatgct
     3661 ttccgccgcc ggaaatgaat attcacagca tcaggggatg agccgggatc gcagtgccat
     3721 gtttggattg atgttctctg tcagtctggg ggcgtcaaaa gagctttggg atagccgccc
     3781 tgaggggagc ggctggagct ggaaggattt tgcctgggat atcgccggtg caagcaccgg
     3841 ctataccgtc tggcaactga cccgtcacta aagccgcatc ccttttcctt tacggtgcaa
     3901 tgttagtgaa accagaaacg ccaccacggc catcaaggtc acataccaga agaaggctgt
     3961 ttccattcct attgatttca gcgacaacgc tacgtactcc gccgaaccac caaatatagc
     4021 attggcgacc gcatatgaca gaccaacgcc taatgcgcga acctgtgccg ggaacatctc
     4081 agccttcagt attccactga ttgaagtata aaaactcact atcagcaggg cgcacatcac
     4141 cagaccaaaa gcggcataag gcgaggaaac gttctgcaat gctgagagaa taggaacggt
     4201 aaaaatggtt gccagagaac cgaaacataa cattgaggta cggcgaccaa tcttatccga
     4261 cagcgcgcca ataagtggtt gaataagcat gaatacaaac aatgcggcag tcataatgcc
     4321 actcgccacg ttggcatgca ttcccgcagt atttaccaga tacttctgca tataagtggt
     4381 gaaggtatag aaacaaaggg agcccgcagc ggtaaaaccg agaaccatga tgaatgcacg
     4441 gcgattgcgc cataatcctt tcagagatcc agcttctttt aaagcgcgtg tttcttgttg
     4501 tgaagtttca tctaactgac gacgtaacca caacgccaca acagctaaca cagcccctaa
     4561 cgcgaaagga atacgccatc cccactctct gagtacagcg tcttccatgg tgtgttgtaa
     4621 aaccacgaca accagtagag ctagcagttg cccgccgatc aacgtcacat actgaaatga
     4681 tgcgtaaaaa cctttgcgcc cttcaacggc aacttcactc atataggtgg cgctggtgcc
     4741 atattctccg ccaacggata atccctggaa taaacgcgcg agaagcaata atgccggagc
     4801 ccaggtaccg attgtttcgt agcctggcaa acaggcgata accagtgagc cgaaacacat
     4861 catacacacc gataacagca tcgatttttt gcgaccatgt ttatcggcta tgcggccaaa
     4921 tagccaaccg cctattgggc gcatcagaaa tcctgcagca aaaacacctg ctgtttgcag
     4981 tagttgggtc gtcgtgttcc cggaagggaa gaagatgtgg gcgaagtaga gtgaacagaa
     5041 cgagtagaca tagaaatcga accactcgac cagattacct gaagaggccc caacaattgc
     5101 ccaaatgcgg cgacgagtat cactacttgt cagtttgctg tctgccatta cagtactttc
     5161 agccatgcca ttatgtctcc tgccgtaatc cgatgctttt gtcggtcgct tttgtttatt
     5221 ttttttggta aagaaaatgt tatacgtttg ttgcgtatca ttatgcaacc ttaaccatga
     5281 atttagttag caggaaaatg gttattaagg agcttaagga tagatttctg gtaaggagga
     5341 agtatggaag tgggcaattt ggggaagccg tattcgctgt tgaatctggc ttatgtggga
     5401 gcgtagatac tgttacggcc agcaagtttt ttcctggtcc agtctcccgg attgcaggca
     5461 tgccagacac agcgccgtca ggcgctgttg tttcatccct tccgcgattt ttacgcagct
     5521 accggattat gccgtgacgc atcgaacggc acgcctgact tcagcactcc atacgccacc
     5581 tgtgccagct tgcgcattat cgagccgaga atcacctttc ctttcttgcc attagctgcc
     5641 aacgcccgtc cccactcagt cttactggtg gctaccattg caggcatata caacggcctg
     5701 cgaagcgaca catgtccggc tttactcaac cggcttgccc ctctcacact actgcctgat
     5761 tcataacgcc gtggtgtcag acccgcaaaa gcggcgaact gtctggcatg ggcaaaacgg
     5821 tccttcagac catgtaagcc agcaataccg cagatgtttt ctctccgata cccgggatgc
     5881 tttccagcag tttcctgcgg tgtttcatat ccggatcatc gtctgtcagg tcttttatct
     5941 gcttctcaag acgcttcagc tctgcttcaa gccacagaag gtgagcatca atgctcggcc
     6001 tctggacttc ccgcgccgtt tcagtgcgat tcagttcctg cctgtgcata tctgtcagcg
     6061 cctggtggcg tactaccagg gcatgcaacg cgcgttcaag cgggtgatgc gcttcccagg
     6121 ctgcagggcg cttctgacga cagaactctg ccagcatgcg gcatccacgg tatcagtctt
     6181 gttacgcagt ccttcactct gagcgaaagc tttacccagt gcaggattaa tgactgacac
     6241 tatgtcgcca gcatcgtaaa ggcactctgc gacaggttcc atataggtgc cggtcgcttc
     6301 gatgcagata tgcgcatggt caatcttgtg acatttcagc caactcacca gctcatcgtg
     6361 ccctttagtg gtgttagcga attttttggt gcgatgacga ccatcaggac gcaacacatc
     6421 gacatccagt ttctctttag cggtgtcgat accgatataa tgaagttcat gttccatagt
     6481 gaaaccaacc ttgcaaatac ggattaccgg gaaaaccggt ctatgatact gtccggttta
     6541 tcacttcggg agaaaggcag tccgccgcat aaatctacca tgcaggttag aacccaaggc
     6601 cggatacggc atgcggactg caggcagaga aaactggccg attttctctg cactggaggg
     6661 ataatacaag gtcggataag atgcggcatg aactgaaccc caaaacctgg acagtttcag
     6721 ttaaccggct ttttgatact c
//
LOCUS       DAWDRC010000160         6721 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5334_length_6721_cov_2.194569, whole genome shotgun sequence.
ACCESSION   DAWDRC010000160 DAWDRC010000000
VERSION     DAWDRC010000160.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5334_length_6721_cov_2.194569"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1968..1977
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2770..2779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5512..5521
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5913..5922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6280..6289
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1681 a         1625 c         1792 g         1573 t
ORIGIN      
        1 agcaagaagt gcaacaatat tcagataaac agactttgca agggagattg aaggaagcgc
       61 caacatttgc acgagcggct aaagcaaacg ctacacatat cgcaaatcca ggaaccgctc
      121 gctaccagca gttcgatgat aatccggtta agcaggtagc gcaaaaccca ctcgtgacgt
      181 ttagccttga cgtcgacacc ggcagttatg cgaatgtgcg gcgtttcctt aatcaagggc
      241 tgttacctcc gccagacgct gtgcgggtgg aggaggtagt caattatttc ccgtctgact
      301 gggatatcaa agacaaacaa tctattccgg cctctaagcc aatacctttc gctatgcgct
      361 acgaattggc acctgcaccc tggaatgaac agcgaacatt gctgaaagtt gatatcctgg
      421 cgaaagatcg caaaagtgaa gagttacccg cttctaatct ggtctttctt atcgacactt
      481 ctggttcaat gatttctgat gaacgtttgc cacttatcca gtcttcgttg aaattattgg
      541 tcaaagaact tcgtgagcag gataacattg ccatcgtgac ctacgctggc gactctcgta
      601 ttgcgttgcc ttctatctcc ggtagtcaca aggcggaaat taatgctgca attgattcgc
      661 tggatgcaga aggcagtacc aatggcggtg ccgggctgga gatggcttat cagcaggcag
      721 cgaaggggtt tattaaaggt ggtatcaatc gcattttatt ggccactgac ggtgacttta
      781 acgttggcat tgacgatcca aaatcgattg aatcaatggt caaaaaacag cgtgagtccg
      841 gtgttactct gtcgacgctt ggcgtggggg atagcaatta taacgaggca atgatggtaa
      901 gaattgccga tgttggtaac ggcaactata gctacattga taccctctca gaggcgcaga
      961 aagtgttgaa cagtgaaatg cggcagacgt tgattaccgt agcaaaagat gtcaaagcgc
     1021 aaattgagtt taaccccgcg tgggtaacgg aataccgtca gataggttat gaaaagcgcc
     1081 aacttagggc agaggatttt aataacgaca acgttgatgc gggtgatatt ggcgcaggta
     1141 aacatataac attgttattt gaattaacgc tgaaagggca aaaagcatca attgataagt
     1201 tacgctatgc cccggataac aaatcagcga aatcggacaa aacaaaagaa cttgcctggt
     1261 taaagattcg ttggaaatcc ccacagggaa aagaaagtca gttagttgaa ttcccgttgg
     1321 cattctcaat aaaggcacct tcagaagata tgcgttttcg cgcagcagtg gctgcatatg
     1381 ggcaaaagtt acgcggttct gaatacctga ataatacctc ctggcagcag atcaaacagt
     1441 gggctcagaa ggcaaaaggg gaagatccac agggttacag agcggaattt attcgcctga
     1501 ttggactggc gaaagatctg gacaattcac aaaactaaaa atgataattc tgtttgcctg
     1561 atatcgacat caaacgtatc cggcaaacag atgaaaaaat gtcaaataaa tgcgttggta
     1621 tcgttaaatt aatctgctaa ctaaccacag ctttcgactt aaatgctaaa caccgcgaaa
     1681 tcattcgcca gttcagtcgc cgggaaaatt gaccgacatt cacgtaacag gtgctgacaa
     1741 cctttgtcat catagcgcga gctgacgtgg gtaatgatta gcttaccgac tccagcctca
     1801 cgggctagtg ccgccgcctg gcgtgtagaa ctatggccgc gactgttggc tttggcctcc
     1861 atcgccatat ccagagtcgc ttcgtgaacc attacgtcga cgcctttagc cagctccagc
     1921 gcggcatcgc aggggcccgt atcgccgaaa atagccagcg ctttaccnnn nnnnnnngca
     1981 tgattgtttt gcccgctttt aattcctgaa acagtgggcc tggcggcacg ccagcagctt
     2041 ttaatgcctg ggcatttaat gcacctggtt tatcatgttc ttcaatacga taaccataac
     2101 attccagtgg gtgttccatc ggataagcgg tcactttgcg caggccatca tcgaaaattt
     2161 cgccagcacc aatttcgaca atttccagcg gataatcggt ccatgagccg ctaatccgca
     2221 gcgcggtctc cacaaattca cggataccgt gcggaccata aatcgttaag ggttggataa
     2281 tgcctgacat agaacgactg cacagtaagc cgggtaaacc aaagagatga tcgccatgaa
     2341 ggtgactgat aaaaatcttg tccagttttc cagggttaaa ggcggtatgc agtagctgat
     2401 gctgggtgcc ttcaccgcag tcaaataacc aaagtccgct ctgggtcgga tgttgcaggt
     2461 taagcaatat tgccgtgaca ttacgcgtac gggttggcac accggctgaa gtacctaaaa
     2521 aaattaattc catgagttca tcacgctgct gaaaatattc cggctagtat aaagcaaaca
     2581 gcacaaagga gccttccatg atcgactggc aagatctgca tcattctgat ttatccgttt
     2641 ctcagcttta tgccttatta caactgcgtt gtgcggtatt tgtagtggaa caaaactgtc
     2701 cttaccagga tatcgatggt gacgatctgg agggagaaaa tcgccacatt ctcggttggc
     2761 ataacgggan nnnnnnnnna cactggtggc gtatgcgagg attctgaaaa gtgacgacga
     2821 actgcaaccc gttgtgattg gtcgggtgat tgtcagcgaa gccttacgcg gtgaaaaaat
     2881 cggtcagcaa ttgatgagta aggcgctgga atcgtgtact cgccgctggc cggagaagcc
     2941 gatttatctg ggggcgcagg cgcatttaca aaacttttat gggcaatttg gtttcatccc
     3001 tgtgaccgat atttatgaag aagatggtat cccacacatt gggatggcac gcgaggtaat
     3061 tcaggcgtaa tcaataaccc ttgtctatag ttagtgacag gttttacaca aatggagaac
     3121 gagaatgtct aatcagtttg gtgatacacg tattgatgat gacctgacgc tgcttagtga
     3181 aacactggaa gaggtgctcc gctcctctgg cgatcccgcc gatcagaaat atgttgagct
     3241 gaaagcgcgt gcagaaaaag cgctggatga tgtgaaaaaa cgggttagcc aggcttcaga
     3301 cagttattac tatcgggcga agcaggctgt ttatcgtgct gatgactacg tccacgaaaa
     3361 accctggcaa ggaattggtg tgggtgcggc cgttgggttg gttctaggac tgttgctggc
     3421 acgccgttaa aacctcccta caccggggta ctgcattttt tttgtcgtac cccgtataat
     3481 gtgaggcttt taacagggag aggtccgcgt gcaatcactt actacggcgc tggaaaatct
     3541 actgcgccat ttgtcgcaag agattccggc gacacccggc attcgggtta tcgatattcc
     3601 gttccctctc aaagacgctt ttgatgcctt gagctggctg gccagtcagc aggtgtatcc
     3661 gcaattctac tggcaacaac gtaatggtga tgaagaagct gccgtcctgg gtgcgattac
     3721 ccgttttacg tcgttggacc aggcacaacg ttttcttcgc cagcacccgg aacacgccga
     3781 cttacgcatc tgggggttga atgcgtttga cccgtcgcag ggcaatttac ttttaccacg
     3841 cctggaatgg cgacgttgtg gcggtaaagc cacgctgcgg ctgacgctat tcagcgaaag
     3901 ctcccttcag cacgatgcaa tcaaggcaaa agaatttatc gccacactgg tgagtatcaa
     3961 gcccctgcct ggattacatt taaccaccac gcgagaacaa cactggccgg acaaaacggg
     4021 ctggacgcaa ttaatcgaac tggcaacgaa aaccatcgcc gaaggcgagc tcgacaaagt
     4081 tgtgcttgct cgggcaaccg acctgcattt cgcaagtccg gtcaatgcgg cagcgatgat
     4141 ggctgccagt cgtcggctca atctgaattg ctaccatttt tacatggcct ttgatggcga
     4201 aaatgctttt cttggttctt caccggaacg gttatggcgg cggcgtgaca aagcgctgcg
     4261 taccgaagcg ctggcgggaa cggtagcaaa tcatcctgat gataagcagg cgcagcagtt
     4321 aggtgagtgg ctgatggcgg atgataaaaa ccagcgcgag aacatgttgg tggtggaaga
     4381 tatctgccaa cgattgcagg ccgataccca aacgctggat gttttaccgc cgcaggtact
     4441 gcgtctacgt aaagtacagc atcttcgccg ctgtatctgg acttcactca acaaagcgga
     4501 tgatgtgatc tgtttacatc agttgcagcc aacggcagca gttgctggct taccgcgcga
     4561 tctggcgcga cagtttatcg cccgtcacga accgttcact cgagaatggt acgccggttc
     4621 tgcgggctat ctctcattac agcaaagtga attctgcgtt tccctgcgct cagcaaaaat
     4681 cagcggcaat atcgtgcggc tatatgctgg cgcaggcatt gtccgtggtt ccgaccccga
     4741 gcaagagtgg caggaaatcg acaacaaagc ggcagggctg cgtactttat tacaaatgga
     4801 atagtaatga gtcgcatcat taccgattca tatcaataat ctatttttgt agctccttat
     4861 acttagccgc aatattgata ccggacaaac tcatgtcagt aagcgcattt aaccgacgct
     4921 gggcggcggt cattctggaa gcattaacgc gtcacggcgt cagacacatc tgtatcgccc
     4981 ctggctcgcg ttctacaccg ttaacgttag cggcggcgga gaattccgca ttcattcacc
     5041 acacccattt cgatgagcgt ggactggggc atctggcact ggggctggcg aaagtcagca
     5101 agcagcctgt ggcggtgatt gtgacctccg gcacggcggt agcaaatctc tatccggcac
     5161 tgattgaagc tgggttaacc ggagaaaaac tgatcctgtt aaccgccgat cgcccgccgg
     5221 agctgattga ctgcggtgcg aatcaggcga ttcgtcagcc gggaatgttc gcctctcacc
     5281 ccacgcacag tatttctctg ccgcgcccga cccaggatat ccccgcacgt tggctggttt
     5341 ctaccatcga ccacgctctc ggtacgcttc atgctggtgg agtccatatc aactgcccgt
     5401 ttgctgaacc gctgtatggc gaaatggacg acaccgggat tagctggcaa cagcggctgg
     5461 gcgactggtg gcaggacgac aaaccgtggc tgcgtgaagc gcctcgtcgg gnnnnnnnnn
     5521 nagcgcgact ggttcttctg gcgacaaaag cgcggcgtgg tggttgccgg gcgcatgagt
     5581 gcggaagagg gcaaaaaagt tgcactgtgg gcgcaaactc ttggctggcc gctgattggc
     5641 gacgtgctgt cgcaaaccgg acagccgctg ccgtgtgccg atctttggtt aggcaatgcc
     5701 aaagcgacca gcgagctgca acaggcgcaa attgtggtgc agctgggaag cagcctgacg
     5761 ggcaagcggc tcctgcaatg gcaggcaagc tgtgaaccag aagagtactg gattgttgat
     5821 gacatcgaag ggcgacttga tccggcacac catcgtggac gccgcttaat tgccaatatt
     5881 gccgactggc tggagctgca tccggcagaa aannnnnnnn nngccctggt gcgttgaaat
     5941 cccgcgcctg gcggaacagg caatgcaggc ggttattgcc cgtcgcgatg cgtttggcga
     6001 agcgcaactg gcgcatcgca tcagcgacta cttgcctgaa caggggcaat tgtttgtcgg
     6061 taacagcctg gtggtgcgtc tgattgatgc gctttcgcaa cttccggcag gttacccggt
     6121 gtacagcaac cgtggtgcca gcggtatcga cgggctgctc tcgactgccg ccggtgttca
     6181 gcgggcaagc ggcaaaccga cgctggcgat tgtgggcgat ctctccgcac tttacgatct
     6241 caacgcgctg gcgttattgc gccaggtttc cgcaccgctn nnnnnnnnng atgccgcaaa
     6301 atgtccattt tgagcacgcc gccgcgatgt tcgagctgaa atatcatcgt ccgcaaaact
     6361 ggcaggaact tgaaacgaca ctagtcgatg cctggcgtac gccgaccacc acggtgattg
     6421 aaatggtggt taacgacacc gatggtgcgc aaacgctcca gcaacttctg gcgcaggtaa
     6481 gccatttatg atcctgcacg cgcaggcaaa acacggcaaa ctaggtttac cctggttggt
     6541 gtttttgcac ggtttttctg gcgattgcca tgaatggcaa gaagtgggcg aggcgtttgc
     6601 cgactactcg cggttgtatg ttgatctccc aggtcacggt ggttcagcga cgatcagcgt
     6661 cgatggattt gatgatgtca ccggcttatt gtgtaaaacg ctggttagtt acaacatcct
     6721 t
//
LOCUS       DAWDRC010000161         6710 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5345_length_6710_cov_2.842374, whole genome shotgun sequence.
ACCESSION   DAWDRC010000161 DAWDRC010000000
VERSION     DAWDRC010000161.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5345_length_6710_cov_2.842374"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1395..1404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1779 a         1826 c         1578 g         1517 t
ORIGIN      
        1 tccggtatca aaattggcgg tcgcctcgac acccttaatc cgcgctttcc cttcattgta
       61 atatttcagg gtgtgatcat cataatcgat caagtcactg acatcgttac gatatccgga
      121 aatacgccag ttaaccccag cagttaagcc ttcaaacgcg ccttcccatt gtttgctttt
      181 ctcaggattc agattcggat taccgtagga gccgtacagt tgcccaagat ttggtgcctt
      241 ataagatgtt ccgtaggaag caataaagcg ataaccttcg atgaactccc aaccggcgct
      301 ggtttgccag gtaccatgac gaccaaactg agaattatcg tcgctgcgtc ctgcgccttc
      361 aaaagtaaaa tcgccgactt gctgcagccc agtcagatag atgccggtat tacgttgatc
      421 atatccatcc tcaacataag ctgtgcccgg tgccgtgctc tgcttctgcc agtcaacacc
      481 cgcaccaata ttaccgtggc caatgatgat gttgtttgcc cactggacgg tgtactgttt
      541 catctcatcg agcgtcgcgg acgaatcata acgaccataa tgcggatcgt agttgtaatc
      601 tttgctatgg ctgtagctgg taatcagttg tgatttaatc agttcgccgt tatagcgcag
      661 tccggcatcc caactttggc tatagagttt acgggtatcg accagtggca agccgggaga
      721 ataatatgcg tcataattgg tacgattatc atagccatag ccgcgcacaa agccgctcca
      781 ggcatcagta aaattatgct ccagcgcgcc ataaagcgtt ttacttaaaa aaccatcgtt
      841 atctggctgc gcttgcgttc cggtattacc ataggcgaca acatcataac catgagtatg
      901 ggcataatcg cccaacagcg ttacccgtgt cttatccccc agttgttgct gtgtagagac
      961 atcatagttc tgataactat tgcttcccca ccctgctgaa atttccgttc cggggtgatc
     1021 gcgcgtcgtg atgatattca cgaccccgcc tattgcatct gaaccataaa cagcggagcg
     1081 cggcccacgg atatattcaa cacgctggac aagcgcaata gggaactgac taagatcagc
     1141 agaaccactc acccccgcca aattcaggcg gacgccatca attaacacca acacatgact
     1201 ggcatttgta ccgcgaataa aaatagatga gagctgacct gaaccgccgt tttgggtgat
     1261 atccacgccc ggaagacggc gcagcacatc attgaccgag gtcgactgcc agcggtcgat
     1321 atcctgacgc gtcacaacag tagttggcgc aagcacagtg ctgcgcggtt gttcaaaacg
     1381 gttagcagta acgannnnnn nnnngagtat ccgggctggt atcctgtgcc caagcggaaa
     1441 atgccgtgac ggaacacgcc gtcagcagcg aagctttttt aatcattgta aagcatccac
     1501 aatagaagaa ggatgccgca ggtttcatca atattacgcg atgatgagaa ccagatgcga
     1561 cgttggccgg caggtcttcg ggcttggagg ggtatctaag atactaagag atgatgactt
     1621 cccaccggat ggcagtgtcc gcataacgca atcatcgcac ctttccttac cgctgcgcgt
     1681 cagctccaga ttcgcactgg attccctatt aactcacagg accggcaagt ggatgctaca
     1741 ggttgtaaca agttactgtc cagacgtagc tcacaaatag gaattcatca agatctggac
     1801 atctgatgag caatccctac aatcgccgcg tactttaatt tttcaggata catcatgacc
     1861 cccgaacacc ttccaacaga acagtatgaa gcgcagttag ccgaaaaagt ggtacgtttg
     1921 caaagtatga tggcaccgtt ttctaacctg gttccggaag tgtttcgctc gccggtcagt
     1981 cattaccgga tgcgtgcgga gttccgcatc tggcacgatg gcgatgacct gtatcacatc
     2041 attttcgatc aacaaaccaa aagccgcatc cgcgtggata gcttccccgc cgccagtgaa
     2101 cttatcaacc agttgatgac ggcgatgatt gcgggtgtgc gtaataatcc ctttctgcgc
     2161 cacaagttgt tccagattga ttacctcacc acgctgagta atcaggcggt ggtttccctg
     2221 ctgtaccata agaagctgga tgatgagtgg cgtcaggaag cggaagccct gcgtgatgca
     2281 ttgcgagcgc agaatctgaa tgtgcatctg attggtcggg caacgaaaac caaaatcgag
     2341 ctggatcaag attacatcga tgaacgtctg ccggtcgcag ggaaagagat gatctaccgt
     2401 caggtagaaa acagctttac ccaaccgaac gcggcgatga atattcagat gctggaatgg
     2461 gcgctggacg taaccaaagg ctcaaaaggc gatttactgg agctatactg cggcaacggt
     2521 aacttttcat tagcgctggc acgcaatttt gatcgggtat tagccaccga aatcgccaag
     2581 ccgtcagtcg ccgccgcgca atacaacatt gccgctaacc atattgataa cgtacaaatt
     2641 attcgtatgg cggcagaaga atttactcag gcgatgaatg gtgtgcgcga gtttaaccgc
     2701 ctgcaaggaa tcgacttaaa gagttatcag tgcgaaacca tttttgtcga cccaccgcgc
     2761 agcggtctgg acagtgaaac cgagaaaatg gtgcaggcgt atccacgtat tctgtatatc
     2821 tcctgcaatc cggaaacgtt atgcaagaat ctggaaacat taagccagac gcacaaggtc
     2881 gaacgtctgg ctctatttga tcagttcccc tacacgcacc atatggagtg cggcgtatta
     2941 ctgaccgcga agtaaaaccc atgccggatg cgcttgcatc cggcatgata ccgattactc
     3001 agcaacttcc tgcttacggt tacgcatctt cgcgccaatc cagaacacca taatgacgga
     3061 cagtactgcc gggaagaagt tagagccgat atccggatat tccgcacgaa ctaccgtgct
     3121 gtacagcagt acgccgagaa taaaacaagc agctgccaga cccggcaagc ctaccggcat
     3181 agtgcgatta agataacgtt gatgcaggca gtaaactgtc aacaccaggg aaataatcgg
     3241 gaatacagaa aatggtacaa tggagctaaa cagcgctgca aaagtaccat tgatcgataa
     3301 gccagcaacc aacgctagca gcagcgtacc tctatcttga tttgcttgtt tcattactcg
     3361 tccttcacat ttcccggaat aattgtggtt ttctcttgtt cacggcgata ccagtaataa
     3421 gcccctttcg aaatcattcg cagctgcagt accagtcgct cttctaattg ccggcgttgc
     3481 tcgacgccga catccaacgc ctcggccccc gcactgaaga caatcgtcac cattgcttcg
     3541 gcttgggttt cagtaaacgc acgcggcata tggttttcga gttccagata gtccgcaagt
     3601 tccgcaatga agtgctgaat ttcacgcgca acggcggcac gaaacgcagc ggaggtgccg
     3661 gagcgttccc gcaataataa ccggaaggcg ttaggattat taccgatgaa ctccataaat
     3721 gtggagaccg aggtgcggat cacactcccg cctttggcga tacgctgacg cgcctgacgc
     3781 atgagttggc gtagcattaa accgctctca tcaaccatgg tcagacccag ttcgtctacg
     3841 tcgcggaaat gccgataaaa agaggtggga gcaatgcctg cttctcgtgc cacttcacgc
     3901 aaactcaggc tggcgaagct gcgttcagca cttaattggc taaatgcggc ttccaccagc
     3961 gaacggcggg ttttttcttt ttgttgcgct cttacgccca tcacgatgtc tgaatccttg
     4021 ccaaaagtac tgctggcact ataccagaga atgaacatac tggattgctc cagtgttgtg
     4081 aatgaacggt aacgcaataa aacatattta ctgcaattct ttggccggtt cttttacgta
     4141 caacaacgcc agcatcgaca gcaacgtgcc tccacccaga taccacgccg gagccgtcag
     4201 actatccagc aacacgatgg attgcgtggc gaaatattgc gcaaaaccgc caaagattgc
     4261 tacgccaatg ctataaacca gcgcgaaacc taatgcccga atccgtttcg gcaacagttc
     4321 agagatcaac aacatcactg gcacaccgcc aagcgtggta aagctgacca tcacgaaaac
     4381 caccgtcagc aacatgccag ggcttgggta attcaccaac agccagaatg acggccagct
     4441 acagagaagc accatcacac gggagattaa aatcagcttt ttacgcccaa ccgaatcaca
     4501 tagcatcccc accaacagcg cgcccacaaa cgtaattacc cccgccagta acatcgctgc
     4561 atgagaatag tgttgaggca ttgccagata cttcgccgcc caggtgccgt aataaaagag
     4621 ggaaatatag gttgctaccg tgctaccaat agcgagtaaa acgccattaa cgatagtcgc
     4681 cttatgttgc aggagcagag aaaacgcgct ttcgctggta gcagcttttt tattgcgcac
     4741 tggctcaggt acgtcatttt ccaggctcag acgcaaccag caaccgatag gtgctaataa
     4801 cacgccaata aaaaacggca cacgccagcc ccattctgcc attacggttt ctgaacctgt
     4861 ggcgaacggt agccatgcgc ttaaacctaa agcgacaaca ccgccaaaag ttgttgccag
     4921 gccttgggtc gccagtgacc aactgctgta aaagccgcga cgatttgccg gagctgactc
     4981 caccagtagc gacattgaag ccccgacttc accacctgcc gcaacaccct gaattagacg
     5041 cgccagcacc agcgttgcgg ttccccaata gcccgccgtt tcatacgtcg gtgcgatgcc
     5101 gatcatcagc gtaccgaggc tcatcagagc aatagtgaac accatcgccg gtttacgccc
     5161 gaagcgatcg gcccaggcac caacgataat cccaccgagc ggacgcataa aaaagctcac
     5221 accaaacgtt gcaaatgcca gtaacagcgc gacgttatga tcggcactgg gtgggaagaa
     5281 aagcgtactg atatagacca caaaaaagtt gtaaacgcca aaatcaaaaa attcgagcgc
     5341 attgccaaac gccgaggcaa agatcacccg cctgccgctg ggctttttct tcacactcac
     5401 ggtttcattt acgatcgccg tcattccatg ttccttgcca aaaagtgttg ttgtgtgttg
     5461 ttttatttat tgcagccagg cttcaaccag tgcgccccag tagctgcttg ccgggaccag
     5521 gcttgcatcg ttaaagtcgt aaccggggtt atgcaccatg caggaacctt ccccgacatc
     5581 gccattaccg aataacagga aaccgccttt gggttgggct tccagcatga aagagaaatc
     5641 ttcactgcac atcaggggtt tgatgccaaa ctcagcccga tcttcaccaa atgttttgca
     5701 cgccacctgc caggcgaagc gcgccatctc ctcgtcattt accaataccg gtgtaccgtt
     5761 aacatgcgta acctcagccg tagcgccaaa gctggcagcc tgggcttgcg ccagagcagg
     5821 aatacgggtc agaagaagtt gtcgggtatc gcggctaaga gagcggacac tgattttcat
     5881 ctcagcactg tcaggaatga cgttagccgc ctcacccgca acaatgctgc cgacggtaat
     5941 taccgccgct tccagcggat cgacattgcg cgacacaatg ctttgtaatg cggtggtgat
     6001 atgtgcggca accagcactg gatcgatggc tttgtgcggg atcgcaccgt gtccgccaca
     6061 accacgaacc gtaatatgga actgatccat tgacgccatc agcgccccag gctggaagaa
     6121 aaacttgccg accggcaaac ccggcatgtt gtgcatcccg aaaatgacat cgcaggggaa
     6181 acgatcaaaa agcccctctt taatcatgat ctcaccgccg ttaatcatct cttccgcagg
     6241 ctggaagata aggcgtaacg tgccgctaaa acgacgggtt tcggcaaaat aacgcgcagc
     6301 ccccagcagt atggtggtat gcccgtcatg cccgcaggcg tgcattaaac ctggatgttt
     6361 actggcccag ggcttgccac tattttcata aatcggcaac gcatccatat ccgcacgcag
     6421 gccaatactt ttctctccgt cgcccacttt taacgtggct accacgccgg ttgtcgccag
     6481 cccttgctca acctcatagc cccactggcg cagtttttcc gcaaccaaag cactggtttt
     6541 aaattcttgt aatcccagct ccgggttttc gtggatctga tggcggatct cgcgaaggtc
     6601 ttcttcaaac ccacgaatat agtattcgat catcggctat tcctttattc acttataaac
     6661 aggctaatta acggatttta ttgatggtat aaatacaaat aactcatcaa
//
LOCUS       DAWDRC010000162         6697 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5359_length_6697_cov_2.394460, whole genome shotgun sequence.
ACCESSION   DAWDRC010000162 DAWDRC010000000
VERSION     DAWDRC010000162.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_6697_cov_2.394460"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    276..285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    704..713
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1606 a         1652 c         1742 g         1677 t
ORIGIN      
        1 gtatcgccgg tgttatgccg gttaaccagt cgatttatga tgccttaacg gtgcttcagc
       61 atcgcggtca ggatgccgcc ggcatcatca ccatagatgc caataactgc ttccgtttgc
      121 ggaaagcgaa cgggctggtg agcgatgtat ttgaagctcg ccatatgcag cgtttgcagg
      181 gcaatatggg cattggtcat gtgcgttacc ctacggctgg cagctccagc gcctctgaag
      241 cgcagccgtt ttacgttaac tctccgtatg gcattnnnnn nnnnntgccc acaacggcaa
      301 tctgaccaac gctcacgagt tgcgtaaaaa actgtttgaa gaaaaacgcc gccacatcaa
      361 caccacttcc gactcggaaa ttctgcttaa tatcttcgcc agcgagctgg acaacttccg
      421 ccactacccg ctggaagccg acaacatttt cgccgccatc gctgctacaa accgcttaat
      481 ccgcggcgcg tatgcctgtg tggcgatgat tatcggccac ggtatggttg ctttccgcga
      541 tcctaacggg attcgtccgc tggtactggg caaacgtgat attgacgata atcgcacaga
      601 atatatggtc gcttccgaaa gcgttgcgct cgatacgctg ggctttgagt tcctgcgtga
      661 tgtcgcccct ggcgaagcga tttacatcac tgaagaaggg cagnnnnnnn nnnggcacga
      721 agctgggcga gaaaattgcc cgcgaatggg aagatctgga tatcgacgtg gtgatcccga
      781 tcccggaaac ttcctgcgat atcgcgctgg aaattgcgcg tattctgggc aaaccgtacc
      841 gccagggctt cgttaaaaac cgctatgttg gccgcacctt catcatgccg ggtcagcagc
      901 tgcgtcgtaa gtccgtgcgc cgtaaactga acgccaaccg cgccgagttc cgcgataaaa
      961 acgtcctgct ggtcgacgac tccatcgtcc gtggcaccac ttctgagcag attatcgaga
     1021 tggcacgcga agccggagcg aagaaagtgt acctcgcttc tgcggcaccg gaaattcgct
     1081 tcccgaacgt ttacggtatc gatatgccga gcgccacgga actgatcgct cacggtcgcg
     1141 aagtagatga aattcgccag atcatcggtg ctgacgggtt gattttccag gatctgaacg
     1201 atctgatcga agccgttcgc gctgaaaacc cggatatcca gcagtttgaa tgctcggtgt
     1261 tcaacggcgt ctacgtcacc aaagacgttg atcagggcta cctcgatttc cttgatactt
     1321 tacgtaatga cgacgccaaa gcagtgcaac gtcagaacga agtggaaaat ctcgaaatgc
     1381 ataacgaagg atgatgcctt cactcagggt gccggtctgg caccctgact tgcaactccc
     1441 gccgaaatcc tgcaaagtct gcctgcaagt ctgacagggc aactatttat gaaacgactc
     1501 attgtaggca tcagcggtgc cagcggcgcg atttatggcg tgcgtttatt acaggttctg
     1561 cgcgatgtca cagatatcga aacgcatctg gtaatgagcc aggcggcgcg ccagacctta
     1621 tccctcgaaa cggatttttc cctgcgcgaa gtgcaggcgt tagctgatgt gacacacgat
     1681 gcgcgcgata ttgctgccag catttcttcc ggttctttcc agacgttggg gatggtgatt
     1741 ttaccctgtt caatcaaaac cctgtccggc attgtccata gctatactga tggcttactg
     1801 acccgtgcgg cagatgtggt gctgaaagaa cgtcgcccgt tggtgctctg cgtgcgtgaa
     1861 acgccactgc acttaggcca tctgcgttta atgactcagg cggcagaaat cggtgcggtg
     1921 attatgcctc ccgttccggc gttttatcat cgcccacaat cccttgatga tgtgataaat
     1981 cagacggtta atcgtgttct tgaccagttt gcggtaaccc ttcctgaaga tctctttgcc
     2041 cgctggcagg gcgcataact ccgctgttgc cctgtttcag ggcaattttg cagccgcgat
     2101 caaatcctcg acattttgtt tctgccattc aatcgaaacg ctgcgattca accgctatac
     2161 ctgctatctt caactttagg acaataatgc aacgtcttat taacatattt aacgttgaat
     2221 gttactgctg tcgtcaagat ggcataagac ctgcatgaaa gagcctgcaa acacacaaca
     2281 caatacacaa cataaaaaag ccattttcac ttgagggtta tgtatgaaga agtcgattct
     2341 cgctctgtct ttgttagtcg gtctctccgc agcggcttcc agctatgcgg cgctaccgga
     2401 gacggtacgt atcggaaccg ataccaccta cgcaccgttc tcatcgaaag atgccaaagg
     2461 tgactttgtt ggctttgata tcgatctcgg taacgagatg tgcaaacgga tgcaggtgaa
     2521 atgtacctgg gttgccagtg actttgacgc gctgatcccc tcactgaaag cgaaaaaaat
     2581 cgacgccatt atttcgtcgc tctccattac cgataaacgt cagcaggaga tagccttctc
     2641 tgacaagctg tacgccgcag attctcgttt gattgccgcc aaaggttcac cgattcagcc
     2701 aacgctggat tcactgaaag gtaaacatgt tggtgtactg cagggatcaa cccaggaagc
     2761 ttacgctaac gagacctggc gtagcaaagg cgtggatgtg gtggcctatg ccaaccagga
     2821 tttagtctat tccgatctgg ctgcaggacg tctggatgct gcgttacaag atgaagttgc
     2881 tgccagcgaa ggtttcctta agcaacccgc cggtaaagat ttcgcctttg caggcccatc
     2941 agtgaaagac aaaaaatact tcggtgacgg caccggtgta gggctgcgta aagatgatgc
     3001 tgaactgacg gctgcgttta ataaggcgct tggcgagctg cgtcaggacg gtacctacga
     3061 caagatggcg aaaaagtatt tcgactttaa tgtctacggt gactgatacg tcgcagggaa
     3121 gctgtacctg atggaatgat catcatggtg cacgccaggt ttgttgcact atcgtggtgc
     3181 attgaaatgc atacttaagc atttttaatg aaaaataata cgtctagcgg ggcgggatat
     3241 tttgccttga tggtcaattt tatggcacga taagtgtaac aaacctgtaa atattcccga
     3301 taaaaagact gtcagttgag gacattatga aaaaactggt gctatcgctc tctctggttc
     3361 tggccttctc cagcgcaact gcggcatttg ctgcgattcc gcaaaacatc cgcatcggta
     3421 ccgatccgac ctatgcgcca tttgaatcaa aaaattcaca aggtgaactg gttggcttcg
     3481 atatcgatct ggcgaaggag ttgtgcaaac gcatcaatac gcaatgtacg tttgtcgaaa
     3541 acccactgga tgcgttaatc ccgtccttaa aagcgaagaa gattgacgcc atcatgtcat
     3601 cgctttccat tacggaaaaa cgtcagcaag aaatagcctt caccgacaaa ctgtacgctg
     3661 ccgattctcg tctggtggtg gcgaaaaatt ccgacattca gccgacagtc gaatcgctga
     3721 aaggcaaacg ggtaggcgta ttgcagggca ccacccagga gacgttcggt aacgaacact
     3781 gggcaccaaa aggcattgaa atcgtttctt atcaggggca ggacaatatt tattctgacc
     3841 tgactgccgg acgtattgat gccgcgttcc aggatgaggt cgcagccagc gaaggtttcc
     3901 tcaaacaacc cgtcggtaaa gattacaaat tcggtggccc gtctgttaaa gatgaaaaac
     3961 tgtttggcgt agggaccggc atgggcctgc gtaaagaaga taacgaactg cgcgaggcac
     4021 tgaacaaagc ctttgccgaa atgcgcgctg acggtactta cgagaaatta gcgaaaaagt
     4081 acttcgattt tgatgtttat ggtggttaat cgccatcggt gaaagaaagc ccgctccctt
     4141 cgggtatccg gagggagaca agaggcggta attcaccaca cacgacagga caggcagcat
     4201 gttgtatggg ttttcaggtg ttattttaca gggtgcgctc gtcacgctgg agctggctat
     4261 cagctctgta gtgctcgctg taatcatcgg tttaattggt gctggcggta agctctcaca
     4321 aaatcggctt tcgggcctta ttttcgaagg gtacaccacg ctgattcgag gcgtgccgga
     4381 tttagtattg atgctgctga ttttctacgg tttgcagatt gcgctaaaca cggtgacgga
     4441 ggcgatgggc gtcgggcaga ttgatattga tccgatggtc gcaggtatta tcactctcgg
     4501 ttttatctac ggtgcttatt ttaccgaaac gtttcgtggc gcgtttatgg cagtgccgaa
     4561 aggacatata gaggcggcga cggcgttcgg ttttactcgt gggcaagtgt ttcggcggat
     4621 catgtttccg gcgatgatgc gttacgccct gccaggcatt ggcaacaact ggcaggtgat
     4681 cctcaaatct accgcactgg tttcgttact cggcctggaa gatgtggtca aagccacgca
     4741 actggcaggc aaaagtacct gggaaccgtt ctatttcgct atcgtctgtg gcgtgattta
     4801 tcttgttttc accaccgttt ccaatggtgt gctgctgttc cttgagcgcc gctactccgt
     4861 gggtgtgaag agggctgacc tgtgatcgaa atcttacatg aatactggaa accactgctg
     4921 tggactgacg gttatcgctt tactggcgtg gcgatcaccc tgtggctgct gattttgtcg
     4981 gtagtgatag gcggagttct ggcgctgttt ctggcgattg gtcgcgtctc aagtaataaa
     5041 tacatccagt ttccaatctg gttatttacc tatatttttc gcggtacgcc gctgtatgtt
     5101 cagttgctgg tgttctattc cggcatgtac acgctggaga ttgttaaggg aaccgagttc
     5161 cttaacgcct tttttcgcag tggtctgaac tgtaccgtgc tggcgctgac gcttaacacc
     5221 tgcgcttaca ctaccgagat ttttgccggg gcaatccgtt cagtgccgca tggggaaatt
     5281 gaagccgcca gagcctatgg cttctcgacc tttaaaatgt atcgctgcat tattttgcct
     5341 tctgcgctgc gtattgcgtt accggcgtac agcaacgaag tgatcctgat gctgcactct
     5401 actgcgctgg catttactgc cacggtgccg gatctgctga aaattgcccg cgatattaac
     5461 gccgccacat atcaaccttt taccgctttc ggcattgccg cggtgctcta tttaatcatc
     5521 tcttatgtcc tgatcagcct ctttcgtaga gctgaaaaac gctggttgca gcatgtgaaa
     5581 ccttcttcaa cgcactgaga atacgatgtc cgagaataaa ttaaacgtta tcgatttgca
     5641 caaacgctac ggcgaacacg aagtgctgaa aggggtctca ttgcaagcga atgccggaga
     5701 tgtcataagc atcatcggat cgtcgggatc gggtaaaagt acctttctgc gctgcattaa
     5761 cttcctcgaa aaaccgagcg aaggatcgat cgtggtcaat ggccagacga tcaatctggt
     5821 gcgcgataaa gacggtcaac tcaaagtcgc cgataaaaat cagctgcgct tattgcgcac
     5881 acgcctgacg atggtattcc agcacttcaa tctctggagc catatgacgg tgctggaaaa
     5941 cgtcatggaa gcgccgattc aggtgttggg cctgagcaag caggaagcgc gcgagcgggc
     6001 ggtgaagtat ctggcaaaag tcgggataga cgaacgtgcg caggggaaat acccggtgca
     6061 cctgtccggt ggtcagcaac agcgtgtttc tatcgcgcgg gcgctagcga tggaaccgga
     6121 agttttactg tttgatgaac ctacttcagc gctcgatcct gaactggtag gcgaagtgtt
     6181 gcgtattatg cagcaactgg cagaagaggg gaaaaccatg gtggtagtga ctcacgaaat
     6241 gggctttgct cgtcatgttt ctactcacgt cattttcctc catcagggga aaatagaaga
     6301 agaaggggcg ccggagcagt tattcggtaa cccgcaaagc cctcgtctgc aacagttcct
     6361 taaaggttcg ctgaaataat ttcctctccg gtggttaacg ctgccggagt atttcatggg
     6421 gaatttgcga gtccgcttcc gctgaaatgt aaaagttgca gtccgctaaa aatagttctg
     6481 aattatctat atttattcat cagcgtttcc gctggcggat gtttgcagta aaccacatca
     6541 gcgaacgaca tccgccagcg cctcttctaa atcgtaccag cgaaacgcaa aacccgcttc
     6601 ttccagcttt tttggcagcg cgcgttgtcc acctaatacc agtactgaag attcgcccat
     6661 taacaaccga atggcggtcg cagggacgcg caaaatg
//
LOCUS       DAWDRC010000163         6691 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5364_length_6691_cov_2.346293, whole genome shotgun sequence.
ACCESSION   DAWDRC010000163 DAWDRC010000000
VERSION     DAWDRC010000163.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5364_length_6691_cov_2.346293"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1881..1890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2694..2703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4937..4946
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1577 a         1645 c         1715 g         1724 t
ORIGIN      
        1 ttataatgca ctggctatat tgcgagtgga atatcagtaa tttttgagcc agtacagagt
       61 cggtttagct atcaccgcaa aataaaatgc aataataggg atgaacagaa tgtaaaacaa
      121 aaaaatattt ttaattctac aggggaaaaa cctggtggaa aatgtaggtt atgtgccgat
      181 gaataaaatg ttgcattaag ccggataata aaaatggaat tagttgatac gttatttgca
      241 agcctctccg gtactgatcc attcactggc gtggatatta cgattgccaa ctgcaaatca
      301 acatactggg atgaaggcat tgttcagcaa ctcattaacc aggtcctgga tgaaggggaa
      361 aagttcgccg gtgctgccgg tcttgaggga ttatctcgat atgatgtcac attaaatatt
      421 ggtttaacgt ccagcaatgt atggccggga ttttccctcg ataccgccac cattagccgt
      481 ctttgtgcct gtggtgctga ctttggattt gacctctata tcagtgatgt accagatgtt
      541 caatgtgatc tgaacacgac aaatgacttc accgtacaat ttacggcgat gctcaacccg
      601 gatgagcggg tgatcatagc aaagcgtccg cttaaaaaat gtgacgcctg gattgaggat
      661 gtttatatct ttcaagtgtt taaagaagcc tggcaatttc aaaacgataa cagtttacgc
      721 ggatttcgcg ataagcaggc tgaactgaaa ctctacgcgc gtcattactc agtggaaaat
      781 tgcacagagg aaagctgttg ggactgtaat tattgcattc ggcccagctt ttccctctct
      841 cgtagtgcca ttattcgcct gaatgcggcg aatgctcgtt ttgtctatca accctttaca
      901 cgcgatcagc gggcgagggg gtaattatgc accccactcc tgccatgcag gcattaattg
      961 agcagatata tcatattttt cgtcgttatc cggcgccgaa gcaatttgtg gtttgttgtg
     1021 aatattgtct gagtcaacag gaacaaaaag cattacggaa tacatcgttg cgagcgattc
     1081 cttattcctt aatcaatgcc tggaatagta gtccaggacc tgatccgcaa aacagcgatg
     1141 aggttcgtta ttttctgcct cgtttactgg agtttgttgc gcaggggcaa tttgacaata
     1201 ttcacgaagt attttctcta cggcgtataa accttgccag taaagaaaac tggcgtgagg
     1261 atgaacggga aatattgcag caatttgctt gccagtatat gactgattgg gtatccgggg
     1321 atgaggctgt cgaattacag tacaagctgg aaatgttttt ccgtgccgat atcgctcttt
     1381 cgccattgct tgatgccatt atttcagttc ccggattttg gtcggcagcg tctcttgcct
     1441 gtttattgaa tacgtatcgt gacggttata tcagagataa ccaggacgat atcgataaag
     1501 ccattaccgc ccaaatcaat gcctgggcct tcaacaatca atccatcctg aaagaacgcg
     1561 cgcggcaggc gattgagaac ccgcttaagc agcccgaaca gggcacacag tatcagatct
     1621 gggaagacga ctggatgatt gatgagtgtt tatgcgcgat gtatgacgca tcatccgaat
     1681 caccaggaaa ataaaaatga ttaaattccg gctttatatt ccccctgtaa ttctcggttt
     1741 tgttatcgta ccattattgg tatggccgac ggttattgcc ttagccgtac ttatattcac
     1801 gttaactttt ctggcggaaa taatattctc ctttccgctc ctggttgtgc gtatttctct
     1861 tcaggaatta caacttgagt nnnnnnnnnn aattctctcg aatttcattc tttatatttc
     1921 tccagagaaa tcagcgttgc tggaaaatat ccgaaatttc tttctgacat ttgtctggct
     1981 tcccctgaat ttttcccctt tttggccgca gccgtggact gattttgtcg gcccgattag
     2041 tgcccagctt ggttttgcgt tgggatatta ttgccagtgg cgtagcaaaa atagaagcca
     2101 taggaagaag tggggcgatt gggtaacgtg cttaagtttg gcgattttag ctctggggcc
     2161 gttattcaat tatttacaat gaatagtcta aggaattcaa atagcccggt tgcacttcac
     2221 aaccgggcat catccttacg acgccagcga caacctctgc tgcgtatatt ccaccagcca
     2281 ctgactcaca cgagattgtt gatccgcatt tagccacatg ccttgtttcg tgcgacgcca
     2341 cagggcatcg tcggcgcggc ggacccattc gtgatccacc agatatttca gctccgcttc
     2401 gtagaactca tgaccgaaat cttcaccgag gtcgcttatc gcccctgcat tgccgagcag
     2461 cagttcgctg ttgctgccgt aagtgcgcgc gtagtgacgt gccagtgatt cggtcaggaa
     2521 tgggtaacgg cggcgcaggc gggcggcgta atcgtcacgg tcgccttcaa tggcaccacc
     2581 cggcagcacg ctctctttcg tccacgccgg accaatgccc tgataatacg gcgtcagttt
     2641 ttccagcgcg tgctccgcca gtttgcggta ggtggtcagc ttaccgccga atannnnnnn
     2701 nnntaccgac agcagcgggg ctttgccgtt ttcatcatgg atatcagggg tgtaatcacg
     2761 ggtaatggcc tgcggcgagt cggactcatc atcacatagc ggacgcacgc cggagtaggt
     2821 ccagacgata tcgtcccggt ttaactgctt tttaaagtgc gtgttataca ctttcaacag
     2881 gtaattgatt tcactctctt caatcttcac tgcttttgga tcgcctttgt attccacatc
     2941 ggtggtgccg atgatggaaa actcgtccat ccacgggatc acgaagacaa tacgtttatc
     3001 ttcgttttgc agaatgtagg cttgcttctg ggtatgcaca cgcggcacca caatatggct
     3061 gcctttgatc aggcgaatgc cataaggcga aggcagatgc atcccgtcgt cgaaaaattg
     3121 tttcacccac gggccggtag cattaaccag gccgcgcgcc tgccatgtat attttttgcc
     3181 ggtatcgata tcttccgcct ccacaatcca caggccgttt tcgcggcgag cagacgtggc
     3241 acgagtacga gtaagcactt caccgccttt acgtaccacc atctgggcgt tggcgagtac
     3301 cagacgggcg tcgtctaccc aacagtcaga atattcgaat ccgcgcttaa tttccggttt
     3361 taacactgag tttgcgccaa aacgcaaacc ggttgatccc ggcaggctgg tgcgcttgcc
     3421 cagatgatcg tacataaaca gaccaatgcg aatcatccac gccgggcgta gatgcggacg
     3481 atgcggcagg cgaaaacgca tcgggaaggc gatatgcggg gccattttca gtagcacttc
     3541 acgttcagcc agcgcctcgc tgaccagtcg gaattcatag tgctcaaggt agcgcaggcc
     3601 accgtgaatg agttttgaac tggcggaaga ggtcgcgcaa gcgagatcct gcgcctccag
     3661 catcagcacg gataaaccgc gtccagcggc gtctgccgcg ataccagcac cattgatgcc
     3721 gccacctatc acaatcagat ctttggtttc catgctgccc tcattcactt tcgttaaagt
     3781 tcataaatgt tcgttatcga acatattagc aaagaatcgc gctttgggta acattgaaaa
     3841 aacattttag agtgatatgt ataacattat ggcgtttatc tgccgcttcg acgtaaactg
     3901 tgcgttaaat ttgcccactt gtttgtaaag aaagagagac gcatggatca gttcgaatgt
     3961 attaacgtta ccgacgcgca ccagaagttg caggaaaaag aggcggtgct ggtcgatatt
     4021 cgcgatccac agagtttcgc aatggggcat gcggcgcagg ctttccattt aaccaacgac
     4081 acattgggcg cgtttatgcg tgataacgac ttcgatacgc cagtcatggt gatgtgttac
     4141 cacggcaata gcagcaaagg cgcggcgcag tatctgctgc aacagggcta cgatgtggtc
     4201 tacagcattg atggcggctt tgaagcctgg cagcgccagt ttcccgcaga ggtggcgtac
     4261 ggcgcgtaac gctttatact gtcccctttt gtgtggaata agcgacagca acgatgttga
     4321 tgattacctc ttttgctaac ccccgcgtgg cgcaggcgtt tgttgattac atggcgacgc
     4381 agggtgttat cctcacgatt caacaacata accaaagcga tgtctggctg gcggatgagt
     4441 cacaggccga gcgcgtgcgg gcggagctgg cgcgttttct cgaaaacccg gcagatccgc
     4501 gttatctggc ggccagctgg caggcgggcc ataccggcag tggcctgcat tatcgccgtt
     4561 atcctttctt tgccgccttg cgtgaacgcg caggtccggt aacctgggtg atgatgatcg
     4621 cctgcgtggt ggtgtttatc gccatgcaaa ttctcggcga tcaggaagtg atgttatggc
     4681 tggcctggcc attcgatccg acgctgaaat ttgagttctg gcgttacttc acccacgcgt
     4741 taatgcactt ctcgctgatg catatcctct ttaacctgct ctggtggtgg tatctcggcg
     4801 gtgcggtgga aaaacgcctc ggtagcggta agctaattgt cattactctc attagcgccc
     4861 tgttaagcgg ctatgtgcag caaaaattca gcgggccgtg gtttggcggg ctttctggcg
     4921 tggtgtatgc gctgatnnnn nnnnnntgat gggctacgtc tggctacgtg gcgaacgcga
     4981 tccgcaaagt ggcatttacc tgcaacgtgg gttaattatc tttgcgttga tctggattgt
     5041 cgccggatgg tttgatttgt ttgggatgtc gatggcgaac ggagcacaca tcgccgggtt
     5101 agccgtgggt ttagcgatgg cttttgttga ttcgctcaat gcgcgaaaac gaaaataatt
     5161 ccagggattt atcaatgaaa caaacacaac gtcacaacgg tattatcgaa ctggttaaac
     5221 agcagggtta tgtcagcacc gaggagctgg tagagcattt ctccgttagc ccgcagacta
     5281 ttcgccgcga tctcaacgag ctggcagagc aaaacctgat cctgcgccat catggcggtg
     5341 cggcgctgcc ttccagttcg gttaacacgc cgtggcacga tcgcaaggcc acccagaccg
     5401 aagaaaaaga gcgcatcgcc cgcaaagtgg cggagcaaat tcccaatggc tcgacgctgt
     5461 ttatcgatat cggcaccaca ccggaagcgg tggcgcacgc gctgcttaat cacagcaatt
     5521 tacgcattgt gaccaacaat ctcaacgtcg ctaacacgct gatggtaaaa gaggatttcc
     5581 gtatcattct tgccggtggc gaattacgca gccgcgatgg cgggatcatt ggcgaagcaa
     5641 cgctcgattt tatctcccag ttccgccttg atttcggcat tctggggata agcggcatcg
     5701 atagcgacgg ctcgctactg gagttcgatt accacgaagt tcgcaccaaa cgcgccatca
     5761 ttgagaactc gcgccacgtt atgctggttg tcgaccactc aaaatttggc cgtaacgcaa
     5821 tggtcaatat gggtagcatc agcatggtcg atgctgtcta caccgacacg atgccgcctg
     5881 cgagcgtgat gcaggtgctg aaggaccatc atattcaact ggagctgtgc tgatcctgca
     5941 ttgcttccca tgtcagacca aaacgcgcca ggtatttgcg tagccgatcc gcgtcattga
     6001 cgctggcttt accctggcgc gaaatgtcaa acagttcacg tccggcagca gaaagcgact
     6061 ttgcctggcg gcagatagcg ataacgtgtt ccagttgcat acggtcgaag agatcgatgt
     6121 tttctgcctc tgcgcccagc aacgccgtaa gcgtggaggg gcgactctcc tgccagttat
     6181 agcgcagacg gttgatctca tcttccactg tatccagtgt gatgcgtccg ctagtggcaa
     6241 aggtggccat ccgcgtgacg ctggcagaaa gttcgcgaaa gttaccgcgc catgttgcct
     6301 ggggagaggt cgcaaaggcc agccaggcac gccgcgcttc ggtgttaaaa cgcacgctgt
     6361 cgccagtgag tgaggcgtgg cgctccactt cataatccag gttcggttcg atatcttcct
     6421 ggcgttggcg taatcctggc aaggtgaagg tccagagatt tattcgcgcg tacaggtctt
     6481 cacgaaattt gccttcggca accaactggc gcaaatcgcg caccgttccg gcgataagct
     6541 gaaaatcact gctcacctgg cgatcgctgc caaatgggta aaaggttttc tcttcaatgg
     6601 cttttagcaa catcgcctgt tcgtctgcgc ccagttcgcc aatctcatca agaaacaaca
     6661 ttccgccgtt ggcgctgcgt aataatcctt c
//
LOCUS       DAWDRC010000164         6655 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5389_length_6655_cov_2.230606, whole genome shotgun sequence.
ACCESSION   DAWDRC010000164 DAWDRC010000000
VERSION     DAWDRC010000164.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5389_length_6655_cov_2.230606"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    952..961
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3526..3535
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5391..5400
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1638 a         1698 c         1680 g         1609 t
ORIGIN      
        1 aagggctgaa tacgcggata ccaaatgagg ttaccccaag gcttgaggag aaaagcaaat
       61 cgccgggttt gagatctggt tccgttattt cttttattga ttgttcggtg aaagagcttt
      121 gatgctggaa tttaacagcc catgttttcg cctcggcatt taccactgtg gctgatgagt
      181 ctggctggct gatatcaacg gtacaggccg aaagtaaaag aaagcagggg agaagcaggc
      241 ggcagtacgc ctttggttta tccattttat acaatccatg taaaaaaggg ccccgaaatt
      301 caggaccctt tctggcatca gcctttaatc tgtttcacca gataatcgac gatgtcacca
      361 gtcttaatta actgtttctc gccgttacga cgatatttat attcgatatc atcgttgtcg
      421 aggttacggt cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac
      481 atcacgcccg gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcgcgcagt
      541 tcgctgtaca gtttctcagc aagttcctgt acgcggaagg atttgtgcat gttcattggc
      601 agaatcgcca cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag
      661 ttctgctcaa tcgccgctgc taccacacgc gttacgccga taccgtagca acccatcgtc
      721 aggatttggt tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg
      781 gtacccagct ggaagatgtg accgacttcg ataccacgtt tgatcagcag cgtaccctga
      841 ccatccgggc ttggatcgcc agccaccacg ttgcggatat cagcaatttc cggggtagcg
      901 acatcgcgat cccagttgat accgaagtag tgtttaccat cgatgttagc annnnnnnnn
      961 ncaccggaat cggcatgttt actggaccca gtgaacccgg accggcttta accacggcac
     1021 gaatttcttc ttcggtcgca aaagtcagcg ggctggcaac ctgcggcagt ttttctgctt
     1081 taacttcgtt cagctcgtgg tcaccgcgca ccagcagcgc aaccagcggg aagctactgc
     1141 cttcaacggc tttaaccagc agagtcttaa ccgttttctc aatcggcaga ttgaactgtt
     1201 caaccagttc cgcgatggtt ttcgcgttcg gcgtatcaac cagcgtcatt tcctgggtag
     1261 cagcagcgcg cggttctttc ggcgcgatag cttctgccag ttcaatgttc gctgcatagt
     1321 cagaagtatc ggagaagacc acatcgtctt caccgctctg cgccagcacc tgaaattcgt
     1381 gagaggcgct accgccgata gaaccggtat cggcctgtac ggcgcggaaa tccagcccca
     1441 tgcggctgaa gattttgctg taggccgcat acattgcatc gtaggtttcc tgcagggatt
     1501 cctgagaagt atggaaagag taagcatctt tcatcaggaa ttcgcgggaa cgcatgacgc
     1561 caaaacgcgg acgcacttcg tcgcggaact tggtctggat ctgatagaag ttcagcggca
     1621 gctgtttgta agagctcagc tcgttacgaa tcaggtctgt aatcacttct tcatgagttg
     1681 ggccgagtac gaacggacgg tcaccacggt cagcaatacg cagcaattcc gggccatact
     1741 gttcccaacg accactctct tgccacagtt cagatggctg aaccaccggc attaacacct
     1801 cgatcgcacc ggcgttgttc atctcttcac gcacgatgtt ttcgactttt ttcagaacgc
     1861 gcacgccggt cggcagccag gtatataacc cggaggccag cttgcggatc atcccggcgc
     1921 gcagcatcag ctgatggctg atcacctcgg cgtcggcagg tgtctccttg agagtggaga
     1981 gcaggtattg gctagtacgc atgttgttac ggttccagtt ggaaggtaga acaggctcaa
     2041 ggcgagcctg ggacaaaaaa agtgatttag tttaccagtg caaaagaaat gtcaaaagag
     2101 aagggcgtga atttaacgcg gttccagcgc aaagacttca aaacctgcgt cggtgacgcg
     2161 ccagcgaacg ttaaaatcat gcagccagac ggcataggtt ttgcccgttt cctcaccttt
     2221 acgatatgcc gggcgcgggt cctgcgccag tacttcgcgg ataaacagcg ttaactgcgg
     2281 ataacgcttc ttcagcgtca aaagctgctt ttcgacctct gcggtaaaac acaccgccat
     2341 ttctgccgct ggcgcacttt gcgcatagct ggcactggca tcgggaagcg attcagcaaa
     2401 ggggagatac ggtttgatat ccactaccgg cgtaccatcg accagatcca gactgccgag
     2461 cttcagaatc acgctatctt tatggcaaac aacctctttc agctctacca gcgacatacc
     2521 aatcgggtta gggcgaaaag tggagcgcgt agcgaaaaca cccattctgg cattaccgcc
     2581 gagacgaggc ggacgcacag tcggacgcca gccgccttcc atcgtttgat gaaagacgaa
     2641 aaggatccat aaatggctga atgcttccag gccgcgaacg gcgtcggcct ggttgtaggg
     2701 agcaatgaga tgtagttcgc cgttggcgct ttttaccaga cctggctggc gcggaacggc
     2761 gaatttttct ttatagggcg agcgaataac gcctatttgc tcgaactgga aactgctcat
     2821 ttcgccgtga tgttaagcgc agaaccgata catacagcct gacgatagca acctggcgta
     2881 ccgctggtga cttcgcagct atgcagtaat accgcattgg ctttcatttt cgaggcgttg
     2941 atttgcatcc gcttacgtgc ggttggaatg ctcggcggag agtcctgatt agaggcctgg
     3001 caagagtcgc cactgacttc accgagatcg cggaacggtt tgccgactaa ttcttctgca
     3061 ttggtataaa ttcggaccga cgtggcgcgc ggcgctttcg gttttgcagg ctccgctttc
     3121 ggctggggtg cagtgctttg aacgggttcg acaggggatc tgcttaacat ggaacagccg
     3181 cttagcatga gtgctactaa acagatcggt aaagcacgca tagtatttcc tcaatgtatg
     3241 atcaaaacgt caatattgaa tcaggcgctt gtaaaaatga caagacgggc aagcgcccgt
     3301 cctgaatgat attacaaatt gtggaaacag cctaaatatt accagccttt aacagcgccg
     3361 ccgttaaaca ctttgtttgc tgcttcgtaa acttcgtcag actgataagc ctgaacgaat
     3421 ttcttcacgt tttcggcgtc tttgttgtct tcacgcgtta cgatcaggtt tacgtacggg
     3481 gactctttat cttcgacaaa gataccgtct ttcgctggag tcaggnnnnn nnnnnatctt
     3541 tcagtttgat caagcccact ttttgcagca gcagcagtga acgaccaagg ttagttgggt
     3601 cgtttggcac ggcaacctgc gaaccatcct gcagttcatc cagtgatttg attttcttgg
     3661 agtaaccagc aatcggataa acaaaagtgt tgcctactgc gaccagtttg tagccacgat
     3721 ctttcagttg ctgatcaagg tacggtttat gctggaaggc gttggcgtcg atatcgcctt
     3781 tgctcaatgc ttcgtttggc agaacatagt cgttgaaggt gaccagctca acgtccaggc
     3841 catatttgtc tttcgcgact ttctgcgcga cttctgcaac ctgctgttcg gcaccaacaa
     3901 tcacgccgac tttaatgtgg tttggatctt tttcatcctg accgcagcct accagtgcca
     3961 gtgatccaat cagggctccc actgccgcaa aggttttgaa tttgaacgcc ataccttatt
     4021 ccttcttcaa ttatttatgt tgtgttgaac gttacttgcg agtgacagcc cggacgatgc
     4081 ggtcgcctgc gaactgaatt aaataaacca gaatgaccag caataccagt accgtattca
     4141 tcaccgtcgc gttgtagccg atgtagccat actgatagcc aatctgacct aaaccaccgg
     4201 caccgactgc accacccatc gcggaataac caaccagggt aatcagggtg atagttgccg
     4261 cattcaccag acccggcaac gcttccggta acagcacttt acggacgatc tgcattggcg
     4321 tggcccccat tgcgcgggaa gcttcaatta acccggttgg gatctccagc agagcgttct
     4381 cgaccatacg ggcaataaac ggtgctgcac caacggttaa cggaacaatc gctgcctgca
     4441 atccaatcga tgtaccgaca ataacgcggg taaacggaat catccatacc agcaagataa
     4501 tgaacgggat ggaacggaaa atgttcacaa tcgcagaaat ggtacgatac aacttcgcgt
     4561 tagcaataat ttgccccgga cgcgtgacat aaagcagaac gccaaccggc agaccaatca
     4621 caaagccaaa aaaaccggat acgaaggtca ttgccagcgt ttcccatacg ccacgaacca
     4681 gcagccacat catcggctca gacataaccc agtacctcta cttttacatg gtgttcctgc
     4741 agccaggcaa tggcggcttg cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg
     4801 ccgaacttaa cgccaccggc gtaatccatc tgcgcgctaa taatgttgtt gttgacgttg
     4861 aaacgacgcg cggtttcaga aagcagtggg gcatcgaccg attgaccggt aaactccaga
     4921 cgcagcatcg gcacgcagtc agtaaatggc tccgcttgca gacgttcctg gtaatcttcc
     4981 gggatatcca gatgcagggt cgactgaata aacttctgcg ccagcggcgt tttcggatgc
     5041 gagaacactt cacttaccgt gtcctgctcg atcagttcgc cattactgat aaccgccacg
     5101 caatcacaaa tgcgcttcac aacgtccatt tcgtgggtga tcaacagaat cgtcaaaccc
     5161 agacggcggt tgatgtcttt aagcagttcg agaatagaac gtgtcgttgc cgggtccagc
     5221 gcgctggtgg cttcatcaca cagcaatact ttaggattgc tggctaacgc acgggcaatc
     5281 gccacacgct gtttctgccc accggaaagg ttcgacggat agctatcatg cttatcacca
     5341 agaccaacca atgacagcaa ttccgtcacg cgacgtttga tttcgtcttt nnnnnnnnnn
     5401 agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga aaatcatacc
     5461 aatctggcgg cgagctttag tcaactcgga ttctgacagc gtggtcagct cctggccatc
     5521 aaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta taagcgtact
     5581 cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca catgcaggct
     5641 gacgttgttc aacgcctgga tggtgcgggt gccctggtgg aacactttgg tgatattcga
     5701 aagttttatc attgattatt tattatcgtc attaagttag tcgtggcatt tcgaatgcct
     5761 ggaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct aaatcaatca
     5821 ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg tcacatgcct
     5881 tattaaggag ctataaaagg tggcgaagag cgtacccgca atttttcttg accgtgatgg
     5941 caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat ttatcgacgg
     6001 tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gcgctggtgg tagtaaccaa
     6061 ccagtctggc attgctcgcg gtaaatttac tgaagcacag tttgaaacgc tgaccgagtg
     6121 gatggactgg tcgctggcgg accgcgatgt cgatctggat ggtatctatt attgcccgca
     6181 tcatccgcag ggtagtgttg aagagtttcg ccaggtctgc gactgccgca aaccacatcc
     6241 ggggatgttt ttgtcagcac gcgattattt gcatattgat atggccgctt cttatatggt
     6301 gggcgataaa ttagaagata tgcaggcagc ggctgcggcg agcgtgggaa caaaagtgct
     6361 ggtgcgtacg ggtaaaccta ttacgcctga agcagaaaac gcggcggatt gggtgttaaa
     6421 tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcgt aatgattaaa
     6481 agatgagctg ttgaaataaa aatgcatttt tccgcttgtc ttcctgagac gactccctat
     6541 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
     6601 aagagaaaaa tcctgaaatt cagggttgac tctgaaagag gaaagcgtaa tatac
//
LOCUS       DAWDRC010000165         6642 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5402_length_6642_cov_2.498710, whole genome shotgun sequence.
ACCESSION   DAWDRC010000165 DAWDRC010000000
VERSION     DAWDRC010000165.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5402_length_6642_cov_2.498710"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1613 a         1837 c         1647 g         1545 t
ORIGIN      
        1 acatcaaagt tattgtttat tgtgtataga aataccgtga gttccacgct gtattacttc
       61 agcggaacac acggtattta gccgtatttg aacttacaat ctcaccggat caatatgcca
      121 gatatgatcg gcgtactctt tgatcgtacg gtcagaagag aagtagccca tattggcaat
      181 gttcagcatc gcttttgcgg tccactcttc ctgacgctcg tagagttcgt cgactttatc
      241 ctgacaatcg acatagctgc gataatccgc cagtacctgg tagtgatcgc cgaagttgat
      301 cagcgaatcg accagatcgc gatagcgacc cggatcttcc ggactgaata caccgctgcc
      361 gatttgcgtc agcacctgat gcagctcctc atctttctcg tagtattcac gcggtttgta
      421 gccctgacga cgcagttctt ccacttcttc cgctgtgtta ccaaagataa agatattgtc
      481 agcaccgaca tgatccagca tctcgacatt cgcaccgtcc agcgtaccga tagtcagcgc
      541 gccgttaagc gcaaacttca tgttactggt gccggaagct tccgtccccg ccagcgaaat
      601 ctgttcagac agatcggccg ccggaatgat caactgcgcc aggctaacac tgtagttcgg
      661 gatgaacacg actttcagct tgtcgccaat ctgcggatcg ttgttgatca ctttcgctac
      721 gtcattgatc aaatgaataa tgtgcttcgc catgtaatag gccgaagccg ccttaccgcc
      781 aaaaatattc acgcgcggta cccacttcgc atccgggtcg gccttgatgc ggttatagcg
      841 ggtgatcacg tgcaacacat tcatcaattg acgtttgtat tcgtgaatac gtttgatttg
      901 cacatcgaac agcgctttcg gatttaccac cacattcagc tgctgggcga tatactccgc
      961 cagacgcttt ttgttctcca gcttcgcctg atgcaccgcg tgattaacca ttgggaaatc
     1021 acagtgttgt tgcagatcat taagcaggct aaggtcggtg cgccagttgc ggcccaggtg
     1081 ttcatccaac acggctgaaa gcgatgggtt cgctaccgcc agccagcgac gcggcgtcac
     1141 accgttggtg acgttggtga aacgacccgg gaagattttc gcaaagtcgg caaatagcga
     1201 ttgcaccatc aggttagagt gcagctccga tacaccgtta actttgtggc tcaccacaac
     1261 cgccagccag gccatacgca cacgacgacc gttggattca tcaatgatcg acgcccgtcc
     1321 cagcagatcg gtatcgttcg gatactgttc ctgcaaggtt ttcaggaaat agtcgttgat
     1381 ttcaaagatg atctgcaggt gacgcggcag aattttaccc agcatatcga ctggccaggt
     1441 ttccagcgcc tcgctcatta gcgtgtggtt ggtgtaggag aagacctgac aacacacttc
     1501 aaacgcgtcg tcccagctaa attggtgctc atcgatcagc agacgcatca tctcaggaat
     1561 cgacagcacc ggatgggtat cgttgaggtg aatcgcaatt ttatccgcca ggttatcgta
     1621 ggttttatgc aactgataat ggcggcttaa aatgtcctga atggtcgagg aaaccaggaa
     1681 gtattcctga cgcagtcgca gctcacgccc ggagtaggtg gagtcgtccg gatacagtac
     1741 gcgagatacg ttctcggagt ggtttttatc ttccactgcc gcgaagtagt cgccctggtt
     1801 gaatttaccg aggttaattt cgctactggc ttgggcactc cacaaacgca acgtgttggt
     1861 cgcgtcggtg tcgtaacctg ggattatctg atcgtaagct actcccagaa tctcttcggt
     1921 ttcaatccag cgcgtttttt taccttcctg ctgaatgcga ccgccaaaac ggactttata
     1981 gcgcgtgttg tggcgtttga attcccacgg gttaccgtat tccagccagt agtcaggtga
     2041 ctctttctgg ctaccgttaa cgatgttctg cttgaacata ccgtagtcat agcggatgcc
     2101 gtaaccgcgc cccggcaacc ctaacgtcgc cagagaatca aggaagcaag ccgccagacg
     2161 tcccaggcca ccgttaccga ggcccgggtc attttcttca tcaatcagct cttcgagatt
     2221 taaccccatc gcttccagtg cgccctgtac atcttcgtaa attcctagcg acaacatggc
     2281 gttggagagc gtacggccaa tcaaaaactc catcgacagg tagtaaacct gacgagtttc
     2341 ttgcgacaac tgggcacggt ttgaacgtaa ccagcgctcc acgagacgat cgcgcacagc
     2401 aaataatgtt gcgttcagcc attcatgttt attggcgacg accgggtcct tgccaatcgt
     2461 aaacatcagc ttgtaagcga tagaatgctt aagagcttct acgctaagcg tgggcgatga
     2521 atatgtaaac ggagcattca tataggcgtt tcctgaaaac tatttcaagc gatagtaaag
     2581 ctcacggtac gacttcgccg cgacctgcca gctaaaatcc attgccatag cctgacgttg
     2641 cacaaaccgc cacagtgaag gacgggacca cagtacaaaa gcacgtcgaa tagcccgtaa
     2701 cagcgaccag gcattactat cttcaaagac aaacccactg gcgacgccat ctgcaaggtt
     2761 ctcaagagaa cagtcagaaa ccgtatcagc aagcccaccg gtgcgccgca ctaacggcag
     2821 cgtaccgtac ttcaatccat aaagttgcgt taagccgcac ggttcaaaac ggctgggcac
     2881 cagaatgacg tccgcgccgc ccataatgcg atgcgaaaat gcttcgtgat agccaatctg
     2941 aacgcccacc tggccggggt attccgctgc cgccgcaagg aaaccttcct gcagcaccgg
     3001 atcgcccgcg ccgagtagcg ccagctgccc gccctgctcc agaagaccag gtaaggcttc
     3061 cagcaccagg tcgagacctt tctggctggt cagacggctc accactgcaa aaagcggcac
     3121 tttatcgtca accttaagcc ccattgcgat ttgtaactgg cgcttatttt ccgctttatc
     3181 ttccaacgta tcgcgggtgt aacgcgaggc caacagtaag tccgtctctg gactccagat
     3241 tttctcgtcc acgccgttca gtacgccgga aagacgccct tcacggtgac gctgttgcaa
     3301 cagaccttcc ataccgtagg caaactgcgg ttcggtgatc tcgcgagcgt aggttgggct
     3361 gactgccgta atgtgatcgg catagtacag accggccttc aggaaagaga tttgtccgtt
     3421 gaattccagc ccatgaatat taaagaatga ccatggcaat tggatgtcat tcatgtgatg
     3481 tgcataaaac atgccttgat aggccaggtt gtgcacagta aacaccgact tcgccggacg
     3541 cccgcgcgcc gccagatacg caggcgcaag gcctgcatgc cagtcgtgcg catgcaccac
     3601 atcaggacgc cagaatgggt caagcccgct ggccatttct gccccaaccc accccagcag
     3661 cgcaaaacgc aatacgttgt cggtataggc aaataagttg gtatcgtgat acgggcttcc
     3721 cggacgatca tagagatgcg gcgcgtcaat caggtatatg ccaaccccgt tgtaatgacc
     3781 gaacaacagc gtgatatgtc cggcgaaggt atcacgacgg gatactacct gcgcatcggt
     3841 cacgccacgg cgaatatcgg gaaatgcagg caacagtacg cgagcgtcaa cgccgtctgc
     3901 gatttgtgct gcgggtaatg ccccaataac atcagccaga ccgccggttt taagcagcgg
     3961 gaacatctct gaacatacat gtaaaacctg cattatcgct cctgtttatg ccctaacttc
     4021 cgtagcattt cgcgcgttac cagcacgatg ccttcttctg aacgatagaa acgacgtgca
     4081 tcttcctctg cgttttcacc aatcaccatg ccttccggaa taacacaagc acgatcgatg
     4141 acgcagcggc gcagacggca cgagcgacct acccatactt ccggtaacaa tacggcggaa
     4201 tcaatgttgc agaatgaatt cacgcgaacg cgcgagaaca gaacggactg caccaccacc
     4261 gaaccggaga tcacacaacc gccggatacc agtgagttaa gggtcatccc gtggctaccg
     4321 gagcgatcct gcacgaattt cgctggcggt aatgattcat tgtaggtgcg aattggccaa
     4381 ttgcgatcgt acatatccag ttccggcacc acagaggcca gatcgaggtt cgctttccag
     4441 taagcttcca gcgtacccac atcgcgccag tacggctcgg catccgggtc ggattgtacg
     4501 caagagagcg ggaacgggtg cgcataggcc agaccggctt cggtgatctt gggaatcaaa
     4561 tctttgccaa agtcgtggct ggagttctca tcgcgatcgt cttcttccag cagttcatac
     4621 agatagtcgg cgtcaaagac gtaaataccc atactcgcca gagatttgct cggatcgttc
     4681 ggcattgacg gcgggttagc tggtttttca acgaattcga taattttatc gttctcatca
     4741 accgccataa cgccaaacgc ggaggcttct tcaatcggta ctggcataca agcaacggtg
     4801 caacgtgcgc ctttttcgac gtggtcgata agcatacgcg agtagtcttg cttgtagata
     4861 tggtcgcccg ccaggatcac cacgtattcc gctttataac ggcggataat gtcgaggttt
     4921 tgggtgaccg catctgcggt gccgcgatac cagttttccc ctttcattct ctgctgtgct
     4981 ggcagcagat cgacaaactc gttcatttct tcattgaaga atgaccagcc gcgctggatg
     5041 tgctgcacca gagtgtggga ctggtactgg gtgatcacgc ccatacgacg gatcccggag
     5101 ttgatgcagt tagacagcgc aaagtcgata atgcggaact taccgccgaa gtgtacggcc
     5161 ggttttgctc gcttattggt taaatccttc aggcgggtac cacgtcctcc cgccagtatc
     5221 agggcaacag atttcaatgg cagctggcgc gccaacatta agtgatcgtt cttctctaaa
     5281 ctaaccatga ctaactcctt ttttatcatc tctggaacac acacaatccg tgtgcaggtc
     5341 cctgccagac agccgtaatc actgggttat cctctccagc gaatggggga atggcgtgcc
     5401 actccccagc aggtaaaaca atctctgtta cctcaagcgt ggcgttaatt gcgatcaaaa
     5461 agcgatccga gagcagaatt tgcagctgtt tcggcccgtt ttgccactca tccgtgctta
     5521 aaggttgagc atatcgattt agccaacgga cattgccgtc gccttcttcc caccagcgat
     5581 tctccatcaa agcgggaatg cgcttgcgca gatggattaa cgcggcggta aatgcggtta
     5641 aaccactgct tgcctgcgac cagtccaacc aggttaattg gttatcctga tagtaggcat
     5701 tgttattgcc atgctggctg tgaccatgtt cgtcaccggc cagtaacatc ggcgtaccct
     5761 gggagagcaa caacgttgtt aacagggcgt gaatgctgtc gcgccgccgt tcaaccagat
     5821 caagagtacc gcctaaccct tctttaccat gattgttact gtaattgttg ttggtcccgt
     5881 cgcgattttc ttctccgttt gcttcattgt gtttatggtt gaagcaaacg cagtcgcgaa
     5941 gcgtaaaacc gtcatgcgcg gtgacgagat taatcgcggc actcggcaga cgatcattac
     6001 gtttaaaaac atcgctggag gcagcaaaac gcccggcaaa cgctcccaga ggtaaatcat
     6061 aatgcagcca gaaacgacgg gctgcatcgc ggaaatgatc gttccactcg gcaaacagcg
     6121 gcgggaaatt tcccacctga taaccaccag gagcgatatc ccacggttca gcaattaact
     6181 tcacctgcga gagcaccggg cagttctgga tagcggtaaa caacggcgca tcctgacgaa
     6241 actctggcgt acggcccatg actgccgcca gatcaaagcg gaaaccatcg acgtggcagg
     6301 tttctaccca ataacgcagg caggcgctgg cataatctac caccgccgga tgactcaaat
     6361 tgagcgtgtt accacaaccg gtccagttgt gataatcgcc gtcttctctt atccaataat
     6421 agctacggtt gtcgatccca cgcagcgaga ataacgggcc gtcgaggtcc agttccgcac
     6481 tatggttgag cacgatatca agaatgactt cgatacccgc tttatgcagt gctttgattg
     6541 catcgcgaaa ctcatccagc gccgtttctg gcgagcaggc atacgccgga tgcagcgcaa
     6601 acatcgccac cgggttgtaa ccccagtagt tactaagccc ca
//
LOCUS       DAWDRC010000166         6624 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5420_length_6624_cov_2.161668, whole genome shotgun sequence.
ACCESSION   DAWDRC010000166 DAWDRC010000000
VERSION     DAWDRC010000166.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5420_length_6624_cov_2.161668"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1869..1878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5409..5418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    6379..6388
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1606 a         1722 c         1758 g         1508 t
ORIGIN      
        1 ggcatcagca tgcagctaca ttaattccgc cgttcgaatg ccagtgcttt acgctcgatc
       61 agacgcatca tcagcgtcag caggccgttg acgaccaggt aaataatccc tgccgcaccg
      121 aacaccatta catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatg
      181 agcgtaatgg tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa
      241 gaagagagcg agcgtttaaa cgcgtacggt agcaggatcg ccagcgtatc ttttttgctc
      301 attcccaggg cgctacagga ctgccattga ccttccggga tcgcgcgaat cgcaccgtaa
      361 aacagctgcg tggtgtacgc cgcactgttc agcgacaacg caatcagcgc acataaccac
      421 ggttctgaca acaaatgcca cagtgccgga tactcctgca acgttgggaa ctggcccggt
      481 ccgtaataaa tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatatag
      541 ccccgtacca gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat
      601 gccagaatca gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgcagccct
      661 ttcatcagtt cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc
      721 gcaggtcaat gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg
      781 ccgccacaat gtaccaggtg aacggttcct gggtacgggt agcgatgctt ttggtttgta
      841 gcattaaatc attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc
      901 actggttacc gagacccggc agcgcatggc gccacatctg cggcatcacc agacggaaaa
      961 agatagccga tttcgacagc cccagcgcct gaccggattc ccactgaccc accggcaccg
     1021 cttttaatgc accacgcagc gtttgcgagg cataggcggc atacagcagt gacagagcga
     1081 tgacaccaca aaggaacggg ctaacgtcga agttctcaat gtccatctgc accgggatct
     1141 gcacgaaccc aagattgata gtgaagccat ccgaaagcgt cagcagcagc tgcgaggagc
     1201 caaaatagat aaacagcacc accagaattt ctggcaggcc acgcagaatg gttaccagcg
     1261 ctgaacctgc ccacgcgaca ggacgccatt tcgcagactc ccataccgca aagaacatcg
     1321 ccagcgccag cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc
     1381 ttgctaaagg aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg
     1441 tttcgtaagt gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagtt
     1501 cagtgttgcc ctggcgtact gcgatgccga ggccagtacc gaagtaatct ttatcggtca
     1561 ctttatcgcc cactgccgcc agtttcgggt tatctttcag ccattcagtt accactgcgg
     1621 tgtcaccaaa tacgctgtcg atacgaccgt tttgcagatc cagttttgcg ttctggtaac
     1681 tgtcatatgg cacggtagtg atttccggat gcttatccat aatgaacttc tggtgtgtcg
     1741 tcccgttctg aacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt
     1801 gctgacccac aaacagggca gagttgtcat agtacggggt ggtaaacagc acctgctttt
     1861 cacgctccnn nnnnnnnnaa tgcctgcgcc aggtcgacat caaaaccaac gatctggttg
     1921 tttgcatcaa tcgattcaaa cggaggatag gaggcttcgg tggcaaaacg aatggtttcg
     1981 gctgctgtgg cggaaagact aaaacctgca attaacgcgg caatcagaac ttttttcatt
     2041 gttgttatcc cgaatcttag tgagagagat agtttttaaa tgcttcggtt tgcggctcgg
     2101 taaagcagct cgcgtcgcct tgttctacga tatgaccgtt ttccatatac accactcggc
     2161 tggcggtttt acgcgccact tcaacttcgt gggtgacgat cacctgggta atattcgttt
     2221 ctgccagctc acgaatgatg ctgacgattt gtgccgtaat ttcggggtcc agtgcggcgg
     2281 tcggttcatc gaacagcagt acctgcggtt ccatcatcaa cgcacgggca atagcaacac
     2341 gctgctgctg acctccagaa agatgcagcg ggtaacgatc gctataaggt ttgagacgca
     2401 gacgttccag cagtttttct gcacgggcca gcgcctgatc tttactcaac cccagtacac
     2461 gacagggcgc ttcaatcagg ttttgctgca cggtcagatg cggccacagg ttgtattgct
     2521 gaaacaccat gccaacgtta cgacgcaaat cgcgaatcgc tttgtcagag ggggttttgg
     2581 tgaaatcgaa atggttgcct gcaatgttga gcgtaccgga gcgcggcatc tcaagcagat
     2641 tgagtacacg cagcagcgag cttttacccg cgccgctggg gccaagtaac accagcgttt
     2701 cgccctgtgg gcaatccagc gtgatatcga acagcgcctg atgcgcgccg tagaagcaat
     2761 taatgccgtt taattgaata ctcattgaca ctcgtatact ggcagtctga tagctattga
     2821 ggtcgaagat agtacctttg acagaataat tatgcaatat ttctgcttta aaagttaaaa
     2881 gcaaagcgca ttattcaata aacatagcac aaaataacgg gggcggtggt cggcgagcat
     2941 aaatgtcggc attcctcacg aaatgccgga caatttacgg ggtttattgg ttgatcaaga
     3001 cgttagcgat tctcgatgga ctgacggagc gtacccgctg tggcatgaac gctaccgcct
     3061 aagtagcgca catcgtcgat gacccaacac tggccttcct gaatcattaa cacttcatcc
     3121 tgccagccct ggtcaccctg cttgagatcc acgcgcaatg gaatgttacg ggcatcacga
     3181 ttagggatag tagatgcact ggcaacgtgg gcgctgtctg gcaaggtggt tcgactggaa
     3241 aacggatcgc tgcttaacaa ttcacgatga ctattatcgc ggctggcatc gctaagtagg
     3301 gttgccagtt tgtcactcaa ataggggcgc agagcggtaa tgtcattgcc gcgatgttga
     3361 atgcggtagt catagaactg ttgagccacg ctatcaggcc ccccctggac acaggaaccg
     3421 gtgcgggggc cgttatcttt ataagctggc gtgacggtgg tacaggcact gagtagcagt
     3481 gcgcaaggga taagcatggt caatttgcta tagcgcatga tgatttcctt ataagcgaac
     3541 accttgaaag cgttctacga taataatgat atcctttcaa taatagcgta tcagtccgat
     3601 aatgctttga agatcgaagg cttagcaaac aaggagatcg atcatgcaat tttctacaac
     3661 cccaactctg gaaggccaga ccatcgttga atattgcggt gtggtgactg gcgaagcgat
     3721 tttaggcgcg aatattttcc gtgatttctt tgccggtatc cgcgatatcg ttggcggacg
     3781 ctccggtgcc tacgaaaaag aactgcgtaa agcgcgggag attgcctttg aggagctcgg
     3841 ctcccaggcg cgagcgctgg gggctgatgc cgtcgtcggt attgatatcg actacgaaac
     3901 ggtcgggcaa aacggcagta tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg
     3961 tcgatgagaa gagtcttctg gctggtcgct gccgctctgt tactggcagg gtgcacaggc
     4021 gaaaaaggca ttgtcgagaa agagggatat cagcttgata cccgacgcca ggcgcaggcg
     4081 gcgtatccgc gcattaaagt gctggtgatc cactacaccg cagatgattt tgatagctcg
     4141 ctggcgacac tgaccgataa gcaggtcagc tcgcattatc tggtccctgc ggtgccaccg
     4201 cgatacaacg gtaaaccgcg catctggcaa ctggtgccgg aacaagaact ggcctggcat
     4261 gcggggatta gcgcctggcg cggggcaacg cgccttaacg acacctctat tggcattgag
     4321 ctggaaaacc gtggctggca aaaatcggcg ggagtgaaat attttgcccc gtttgaaccg
     4381 gcacagattc aggcgcttat tccgctggcg aaagatatta ttgcccgtta tcacatcaag
     4441 ccggaaaacg tagtagcaca tgcggatatt gcgccgcagc gcaaagacga tccggggcca
     4501 ttattcccct ggcagcaact ggcacagcag gggattggtg catggccgga tgcgcagcgg
     4561 gtgaactttt accttgccgg gcgcgcgcca cacacaccgg tagagaccgc gtcattgctg
     4621 gagcttttgg cgcgttatgg ttatgacgta aagcctgata tgactccgcg cgagcagcgg
     4681 cgcgtgatta tggcgttcca gatgcatttc cgcccgacgt tatataacgg cgaagcggat
     4741 gcagaaactc aggcgattgc cgaagcgttg ctggagaaat acgggcagga ttagcgcggc
     4801 agttttccgt ggtcgcgcag ccaggcggca gttttctcga taccttcatc cagggtaagg
     4861 actggctgat aacctagctc ttcctgcgcg cgcgtaatat ccagcgtaaa gtcaaaattc
     4921 aacttggaga cgccgtagtg ggttagcggc ggctctttag ctgacttgcg acctaaacgc
     4981 tccatgctgc gggcgatcat atccagcatt gggtagggga cggaacgaat acgacagtca
     5041 atattcaact cgtcgatcag cttctgcacg atgctgcgca gtgtgcgatg ttcgccgttg
     5101 gtgatgttgt acacacgccc ggaaggtagc ttatcgcacg cttcctggct tgccagccac
     5161 atggcgtgca cggcattttc atagtaggtc atatcgacca gcgcactgcc gccatgcggt
     5221 aacagaatac tgccgtagtg atgcatcata tgcgccagac gggggataaa gactttatcg
     5281 tgcggtccga acagactttg tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc
     5341 gaaagcatat tgatcacttc ttcgctggct gctttgctac gggcaaactc gttagcgaag
     5401 cggtgaggnn nnnnnnnngg agggggaaga gatatgaata aagttacgca caccccaggc
     5461 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
     5521 tgtcccccag ggcgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
     5581 cactttagct tgtgatgaga ccaactcggt cagatccgcc ggaacaaact ctgcgcccat
     5641 tttttccagc aatttaccca ttgcctcgtt gcgaccggtc gctcgcacgc tgatgccttt
     5701 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
     5761 cttcatatca atccactgtt gttgagaaaa taacgtgcgc attcttccgt gatttcctcc
     5821 atgatgcaat gggaaacatg aaagaataac gcaggttttg ttgattaatc tgtgctttgt
     5881 tctgccagtc tggcgattcg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
     5941 atcaataacc agtaaaacaa ccctggcata ccgtgcggat gccagaatgc gcgaacatcg
     6001 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
     6061 ttcatgccaa ataacagcgt aagttgtttt tgcggttcaa cgacaatgac tttccagcta
     6121 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagcttatga
     6181 ccaatcgcaa ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
     6241 tctttaccgc cgttttggtt cactacccgc cataaagccg taaggctggc ggacgtttta
     6301 acggtaaacc ccgcctgttt ggcgaaataa ccgtattctg gtcgccagcg agcaaaagcc
     6361 tgcgcgtcat agccccagnn nnnnnnnncg gttgtggaat gagtgcacgt agcgcggtgt
     6421 catccgccag cagatcgtgt ttcagcccct gaatcaacgc cctggcggtg gtgggcggta
     6481 ctgaagtaat cacattaaga aaccacaccg aaatccagcg ggtggggagg gggatgggga
     6541 gcaaccagcg gcgcttaccg ctcaccgcca taaaatgttc aaactgttgc tgataactga
     6601 gcacttctgg tccggcggct tcga
//
LOCUS       DAWDRC010000167         6577 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5463_length_6577_cov_2.089236, whole genome shotgun sequence.
ACCESSION   DAWDRC010000167 DAWDRC010000000
VERSION     DAWDRC010000167.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5463_length_6577_cov_2.089236"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    269..278
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1141..1150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1763..1772
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2793..2802
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1651 a         1742 c         1628 g         1516 t
ORIGIN      
        1 ctggtcgatg gaaaacgtcg tggccttcgg cgataacttc aatgatatta gtatgctgga
       61 agccgctggt acaggcgtgg cgatgggcaa cgccgatgac gcagtaaaag cgcgcgccaa
      121 cattgtgatt ggtgataaca ccaccgacag cattgcccag ttcatttata gccacctgat
      181 ttaatcaggc ggttatcgac acacttttaa tttgcgcata cagccacagg ccaggtttga
      241 tcgccagttc atccctggcc cacgggctnn nnnnnnnnct ggccgttgtc gtcataacta
      301 ttaacaactt ttgcccgcaa tacgttacga atgctggttt gctgcggcgg ttgtaaaacc
      361 agcgagacat cggaagcctg aatgcggatg cgtagcgcag cttgcagcgg ttcgtccagc
      421 ttattgaccc acaaatgctg atcgcccagc gccagcgcgg tcatcgcgta atgcggatga
      481 tgctcaagta ctgtcacttt cagaatgcta ctttgttgct ctttcggtag ccacggattc
      541 atcacgctac taccccagac ttcttccagc gcgccaaagg ctttcacctg accgttttcc
      601 agtaccatca ctctgtctgc cagatggagg atttcatcca gcgaatggct gacatacaac
      661 atcggaatgt tgatttcccg cgtcagacgt tgcagataag gcaacagttc gcgtttacgc
      721 ggaatatcca gtgacgccag cggttcatcc agcaacagca attccggtgc tgtcagcaaa
      781 gcccgaccaa tcgccacgcg ctgtttttcg cctccggaca ggctgcctgg taaacgatca
      841 agcaacggtt caatgcctaa aagcgccacc agcttatcga actgattgac catgcttttc
      901 gccatgccgt agcgcaaatt gccacgcact ttataatgcg gaaacagccg cgcatcctga
      961 aacacatagc ctacgcgacg cttttctggc gtcaggcaga tacctttatc ggcgtcattt
     1021 agtacccgac cattgaggac aatccgccct ttttgcggac gtgtcagccc actgatggcg
     1081 ttaatcagcg aggtttttcc ggcaccagag acaccaaaaa tagcggtgat gccattggcg
     1141 nnnnnnnnnn caatagaaat aatacacagt ctcgccgcgc cactttcccc gccaggggtc
     1201 tggatcaggg tatacatggc agaaggaatg gtccgcgttt caccaggaat gttcgacaca
     1261 aaggtgatgg ttgcgccaaa ttcaccgagc gaacgagcga atgccagcac cgtaccaaca
     1321 ataattcccg gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggcc
     1381 cccagtgttc ttgcggcctg ctccagtttg atatcaacac cttccagcgc cagacgaatc
     1441 gcccgcacca tcagcggaaa agacatgaca gcggcagcga gaaccgcacc gcgccagcta
     1501 aacgcgaaag aaagaccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc
     1561 atcgaaacta ataataggta accgacgacc acgggcggta acaccagcgg tagatgcagt
     1621 acactgtcga gcagagcttt gcccggaaac gtgcaacgta ccagtaacca ggcaaaaaag
     1681 atcccaaacg gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact
     1741 gcctgccatt ctggatcggt cannnnnnnn nntcattact tggttgtaaa tccgtaacgt
     1801 ttaaagattt ccgcagcctg cggccccttc agataatcgt aaaatgcttt cactgtcgca
     1861 ttgttatgcc cttccacaac agcaaccgga tattccactt ttttatgtga atcttccggg
     1921 aaggtggcaa ccacttttac ccctttgctg gcaactgcgt cagaaccgta aacaataccc
     1981 agcggcgctt cattgcgttc gaccaacgcc agcgcgccac gaacatcttc cgccggagcc
     2041 agtttcgggg agagcgtatc ccatgcgccc agtttttgca gtgcttcttt cgcataaatg
     2101 ccagcaggga catgttccgg atcaccaacc gcgaggcgac cgccattcag cagcgaagtc
     2161 cagttggttt tgctgtcgat ggtgaaatct ttctgctcgc tggctttcgg tgctacaacg
     2221 accaggctat tgccgagcag tgtctggcgc gaagccgtat cgatcgcttt tttatcaacc
     2281 gcataatcca tccatttctg atcggcagaa ataaacagat ccgcaggcgc acccgcttca
     2341 atctgacggg ccaaagtaga tgacgaagcg aaagaagaaa ccacatccac gcctttctct
     2401 tttttatact gcgtagcaat gtcctgcatt gcgttagtca gtgatgcggc ggcgaacacc
     2461 gtgattttcc cttcatctgc cagtgcattg ccagcaacag cgaaagagag tgccgccccg
     2521 gtaaacaagt tcaaccattt acgagccatt ggtaacccct gaatgtaacg ttatgtaggc
     2581 gtcaatataa cggcaagttc ggaattttcc cagcagttat tgctaacctt tgtgaggtag
     2641 atgagaaaaa atatcggcag aaaaagcagg aagttgagaa aaagaaaatg cccgactaag
     2701 cgggcattca gggaatcaat gattttgtcc gggctggtct tttttaccaa caccagaaaa
     2761 gatgttgaat acttgaccaa gaccgtaaat cannnnnnnn nnatggccat cacgacaggt
     2821 accatgatta cggcgaatac cagacttttt aataactcta acatggtcaa ctccagatat
     2881 agtcatgaga ctattctaac cgctaagcac agaaaagcac tccccttttg tgcggtcagc
     2941 tttgcgtggc gttcgttttc cgtcacaata agacttttgc caggacattg ttatgcaggc
     3001 cgaaatcctt ctcaccctta agctccaaca aaaattattc gccgacccgc gccgcatttc
     3061 gctactaaaa cacattgcgc tttccggttc cattagccag ggagcgaaag atgccggtat
     3121 cagctataaa agcgcctggg atgccattaa cgagatgaat cagttaagtg agcatattct
     3181 ggttgagcgc gcaacaggcg gtaaaggtgg aggcggcgca gtactgaccc gctatggtca
     3241 gcgactgatt cagctctatg acttactggc gcaaatccag caaaaagcct ttgatgtgtt
     3301 aagtgacgat gacgccctgc cgctgaacag cctgctggcc gcgatctcac gtttttcact
     3361 gcaaaccagc gcccgtaacc agtggttcgg taccatcacc gcccgcgatc atgatgacgt
     3421 tcaacagcat gttgatgtct tactggctga tggaaaaaca cgcctgaaag tcgcaattac
     3481 cgcacaaagc ggcgcacgtc tggggctgga tgaaggcaaa gaagtgttga tattgctaaa
     3541 agcgccgtgg gtagggatta ctcaggacga ggcggtcgcg caaaacgctg acaaccaatt
     3601 accgggtatt attagtcata ttgagcgcgg cgcagagcag tgcgaagtat taatggcgct
     3661 acccgacggg caaacactgt gcgccacagt gccgataaat gaagcgactt ctcttcagca
     3721 aggacagaat gtcacggcct actttaatgc cgacagcgtg attatcgcca cgctgtgcta
     3781 agcgtgttga caatttgtta tgaaacacgt atccctgtca gtaatcgctg cacaaaatgg
     3841 gatataaaat gtcatcgttg caaattttgc aaggcacgtt tcgtcttggc gacacaaaaa
     3901 cgctgcaatt gcctcggcta acgttaaacg cgggtgatag ttgggcgttt gtcggttcga
     3961 atggaagcgg gaaatcggcg ctggcccgcg cgctgtcggg ggaacttccg cttttgaaag
     4021 gtgaacggca aagccagttt tcccacatca ctcgtctctc cttcgagcaa ttgcaaaagc
     4081 tcgtcagcga cgaatggcag cggaataaca ccgatatgct cggccctggc gaagatgaca
     4141 ccgggcgtac cacggctgag atcattcagg atgaagtaaa ggatactccg cgttgcatgc
     4201 aactggcgca gcagttcggt attaccgccc tcctcgaccg acgctttaaa tacctttcca
     4261 ctggcgagac gcgaaaaacc ctgctgtgtc aggcgctgat gtcggagcct gacttgttga
     4321 tccttgatga accgttcgat ggcctggatg ttgcctcacg tcagcaactg gctgagctac
     4381 tcgcctcgtt acatcagtcc ggtattactc tggtactggt gctcaatcgc ttcgatgaga
     4441 tcccggaatt tgtccagttt gctggcgtgc tggcggattg cacgttagcg gaaactggcg
     4501 ctaaagaaga actgctccaa caagcactcg ttgcgcaact ggcgcatagc gaactgcttg
     4561 aaggtgtgca aatgccggag ccggatgaac cttcagcacg tcacgcctta cccgccaacg
     4621 aaccgcgcat tgtgctgaac aatggcgtgg tttcttataa cgatcgcccc attcttaata
     4681 accttagctg gcaggtgaat ccaggcgaac actggcaaat tgtcgggcca aatggcgcgg
     4741 gaaaatcgac gttattaagc ctgattactg gcgatcatcc gcaaggttac agcaacgatt
     4801 tgacgctttt cggacgacgt cgcggcagcg gcgaaaccat ctgggatatc aaaaagcata
     4861 tcggttacgt cagcagtagt ttgcatctgg attaccgggt cagcactact gtgcgtaatg
     4921 tgattctttc tggctatttt gattcgattg gcatttatca ggccgtttcg gatcgccagc
     4981 aaaaactggt gcagcagtgg ctggatattc tcggcattga taaacgcacg gctgacgctc
     5041 ctttccatag tctttcctgg ggacagcagc gtctggcgct gatcgtccgt gcgctagtga
     5101 aacatccgac gttgcttatt ctcgatgaac cactacaggg gcttgatccg ctcaatcgcc
     5161 agcttatccg ccgttttgtt gatgtgctga ttagcgaagg tgaaacgcaa ttgttgtttg
     5221 tttcgcacca cgctgaagat gcgcctgcct gtattaccca tcgtcttgag ttcgtgccgg
     5281 acggtgactt ttatcgctat gcgctgacaa aaataaactg agtcggtagt gctgaccgtg
     5341 ccggaggcgg cgtaaacgtg ttatccggcc aacggtttga cgcatgcagg catgaaaccg
     5401 cgtctttttt tcagataaaa agcgcaatca ttcataaacc ctctgtttta taatcactta
     5461 atcgcgcata aaaaacggct aaattcttgt gtaaacgatt ccactaattt attccatgtc
     5521 acacttttcg catctttgtt atgctatggt tatttcatac cataagccta atggagcgaa
     5581 ttatgagagt tctggttacc ggtggtagcg gttacattgg aagtcatacc tgtgtgcaat
     5641 tactgcaaaa cggtcatgat gtcatcattc ttgataacct ctgtaacagt aagcgcagcg
     5701 tactgcctgt tatcgagcgt ttaggcggca aacatccaac gtttgttgaa ggcgatatcc
     5761 gtaacgaagc gttgatgacc gagatcctgc acgatcacgc tatcgacacc gtgatccact
     5821 tcgccgggct gaaagccgtt ggcgaatcgg tacaaaaacc gctggaatat tacgacaaca
     5881 atgtcaacgg caccctgcgc ctgattagcg ccatgcgcgc cgctaacgtc aaaaacttta
     5941 tctttagctc ctccgccacc gtttatggcg atcagcccaa aattccatat gttgaaagct
     6001 tcccgaccgg cacaccgcaa agcccttacg gcaaaagcaa gctgatggtg gaacagatcc
     6061 tcaccgatct gcaaaatgcc cagccggact ggagcattgc cctgctgcgc tacttcaacc
     6121 cagttggcgc gcatccgtcg ggcgatatgg gcgaagatcc gcaaggcatt ccgaataacc
     6181 tgatgccata catcgcccag gttgctgtag gccgtcgcga ctcgctggcg atttttggta
     6241 acgattatcc gaccgaagac ggtactggcg tgcgcgatta catccacgta atggatctgg
     6301 cggacggtca cgtcgtggcg atggaaaaac tggcgaacaa gccaggcgta cacatctaca
     6361 acctcggcgc tggcgtgggc agcagcgtgc tggacgtggt taatgccttc agcaaagcct
     6421 gcggcaaacc ggttaattat cattttgcac cgcgtcgcga gggcgacctt ccggcctact
     6481 gggcggacgc cagcaaagcc gaccgtgaac tgaactggcg cgtaacgcgc acactcgatg
     6541 aaatggcgca ggacacctgg cactggcagt cacgcca
//
LOCUS       DAWDRC010000168         6543 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5491_length_6543_cov_2.247842, whole genome shotgun sequence.
ACCESSION   DAWDRC010000168 DAWDRC010000000
VERSION     DAWDRC010000168.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5491_length_6543_cov_2.247842"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4174..4183
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1530 a         1612 c         1837 g         1554 t
ORIGIN      
        1 aatcgcccag tggcaactcc agccgctacc cattagtggc ttatgttact tcgacaatgc
       61 gttgtggatc cgccttgagg gcggcgaagg atcggtaaaa gcagcgcgtg aactgctggg
      121 tggcgaagag gttgcaggtc agttctggca gcaattgcgt gaacaacaac tgccgttctt
      181 ctcgttacca ggtaccttat ggcgcatttc attacccagt gatgcgccga tgatggattt
      241 acccggcgag caactgatcg actggggcgg ggcgttacgc tggctgaaat cgacagccga
      301 tgataatcaa atccatcgca tcgcccgcaa cgctggcggt catgcgaccc ggtttagtgc
      361 cggagacggt ggctttgccc cgctaccggc acctttattc cgctatcacc agcagcttaa
      421 acagcagctc gacccttgcg gcgtgtttaa ccccggtcgc atgtacgcgg aactttgagg
      481 agcaggctat gcaaacccga ttaacagaag agatgcggca gaacgcgcgc gcgctggaag
      541 ccgacagcat cctgcgcgcc tgtgttcact gcggattttg taccgcaacc tgcccaacct
      601 atcagcttct gggcgatgaa ctggacgggc cgcgcgggcg catctatctg attaaacagg
      661 tgctggaagg caacgaagtc acgcttaaaa cacaggagca tctcgatcgc tgtctcactt
      721 gccgcaattg tgaaaccacc tgtccttctg gtgtgcgtta tcacaatttg ctggatatcg
      781 ggcgtgatat tgtcgagcag aaagtgaaac gcccactgcc ggagcgaatg ctgcgcgaag
      841 gattgcgcca ggtagtgccg cgtccggcgg tcttccgtgc gctgacgcag gtagggctgg
      901 tgctgcgacc gtttttaccg gaacaggtca gagcaaaact gcctgctgaa accgtgaaag
      961 ctaaaccgcg tccaccactg cgccataagc gtcgggtttt aatgctggaa ggctgcgccc
     1021 agcctacgct ttcgcccaac accaacgcgg caactgcgcg agtgctggat cgtctgggga
     1081 tcagcgtcat gccagctaac gaagcaggct gttgtggcgc ggtggactat catcttaatg
     1141 cgcaagagaa agggttagcg cgggcgcgca ataatattga tgcctggtgg cccgcgattg
     1201 aagcaggtgc cgaggcaatt ttgcaaaccg ccagcggttg tggcgcgttt gtcaaagagt
     1261 atgggcagat gctgaaaaac gatgcgttgt atgccgataa agcgcgtcag gtcagtgagc
     1321 tggcggtcga tttagtcgaa cttctgtgcg aggaaccgct ggaaaaactg gcaattcgcg
     1381 gcgataaaaa actggccttc cactgcccgt gtaccctaca acatgcgcaa aagctgaacg
     1441 gcgaagtgga aaaagtgttg cttcgtcttg gatttacctt aacggacgtc cccgacagcc
     1501 atctgtgctg cgggtcggcg ggaacgtatg cgttaacgca tcccgatctg gcgcgccagc
     1561 tgcgggataa caaaatgaat gcgctggaaa gcggcaaacc ggaaatgatc gtcaccgcca
     1621 acattggttg ccagacgcat ctggcgagcg ccggtcgtac ctctgtgcgt cactggattg
     1681 aaattgtaga acaagccctt gaaaaggaat aacaaaatga aaactaaagt cattcttagc
     1741 cagcaaatgg cgagtgcaat tattgccgca ggtcaggaag aggcgcagaa aaataactgg
     1801 tctgtttcca ttgctgttgc cgatgacggc ggtcatctgc tggcgttaag tcgcatggac
     1861 gattgcgcgc cgattgcggc ttacatctcc caggagaaag cgcgtaccgc cgcgctgggg
     1921 cgtcgtgaaa ctaagggcta tgaagagatg gtgaacaacg gacgtaccgc gttcgtgact
     1981 gcgccgttac tgacgtcgct ggaaggtggc gtgccggttg ttgtggatgg gcaaattatt
     2041 ggtgccgtgg gcgtttctgg tttaaccgga gcacaggatg cgcaggtcgc gaaagcggca
     2101 gcagcggtgt tggcgaaata agcgaaaacg aggagataaa caatgagtca aaccataacc
     2161 cagggccgtt tacgcattga cgccaatttt aaacgttttg tggatgaaga agttttgcct
     2221 ggcgtggaac tggatgctgc cgcgttctgg cacaatgttg atgagatcgt tcacgatctg
     2281 gcgccagaga atcgtcagtt gctggcagag cgcgatcgca ttcaggcggc gcttgatgag
     2341 tggcatcgca gcaatccggg gccggtaaaa gataaagcgg cctataaatc tttcctgcgt
     2401 gaactgggct acctggtgcc acagcctgac cacgttacgg tggaaaccac gggcatcgac
     2461 agcgaaatca ccagtcaggc ggggccacag ctggtggttc cggcgatgaa cgcccgctac
     2521 gcgctgaacg cggcgaacgc tcgctggggc tcactgtacg atgcgttata cggcagcgac
     2581 atcatcccgc aggaaggggc gatggtcagc ggctacgatc cgcagcgcgg tgagcaggtt
     2641 atcgcctggg ttcggcgttt cctcgatgaa tcactaccgc tggaaaatgg cagctatcag
     2701 gatgtggtgg cgtttaaggt ggtcgataaa caattacgca tccagttgaa aaatggtaaa
     2761 gaaaccacgt tacgtactcc agcacagttt gtcggttacc gtggcgatac tgctgcgctg
     2821 acctgcattt tgctgaaaaa taacggccta catattgagc tacaaatcga tgccaacggg
     2881 cggattggca aagacgatcc ggcgcacatc aacgatgtta tcgtcgaagc ggccatcagt
     2941 accattctcg actgcgaaga ttcggtcgcg gcggttgatg cggaagataa aatcctgctg
     3001 tatcgcaacc tgctgggcct gatgcagggg actctgcaag agaaaatgga gaagaacggt
     3061 cggcaaatcg tgcgtaaact gaatgacgat cgtcagtaca ctgccgccga tggatctgaa
     3121 atttctctgc acggacgctc gttgctgttt atccgcaacg tggggcattt gatgaccatt
     3181 cctgtgattt gggacagcga aggcaatgaa atcccggaag gcattcttga tggcgtcatg
     3241 actggcgcga ttgccctcta tgatttaaaa gtgcagaaaa actcgcgcac tggcagcgtc
     3301 tatattgtga aaccgaaaat gcacggtccg caggaagtgg cgttcgccaa caaactgttt
     3361 tcccgcgttg agacaatgct cggtatggca ccgaataccc tgaaaatggg cattatggat
     3421 gaagaacgcc ggacctcgct gaacttgcgt agctgtatcg ctcaggcgcg caaccgcgtg
     3481 gcgttcatca ataccggttt cctcgaccgt accggcgatg aaatgcattc ggtgatggaa
     3541 gcgggcccga tgctgcgtaa aaaccagatg aaatcgacgc cgtggatcaa agcctacgaa
     3601 cgtaataacg tgctttccgg tctgttctgt gggctacgtg gaaaagcgca aatcggtaaa
     3661 ggcatgtggg caatgccgga cctgatggca gacatgtaca gccagaaggg cgaccaattg
     3721 cgtgccgggg caaacacggc ctgggttccg tcgccaactg ccgctacgct ccatgcgctg
     3781 cactaccacc aaaccaacgt acagagcgtg caagccaaca ttgcccagac cgagttcaat
     3841 gctgaatttg aaccgctgct ggacgatctg ctgactattc cggttgctga aaacgctaac
     3901 tggtcggcgc aagagatcca acaagagctg gataacaacg tgcaggggat tctggggtac
     3961 gtggtgcgtt gggtggagca ggggattggt tgttcaaaag tgccggatat tcacaacgtg
     4021 gcgttgatgg aagaccgcgc aacgctgcgt atctccagcc agcatatcgc caactggtta
     4081 cgtcacggta ttctgaccaa agaacaggtg caggcctcgc tggagaatat ggcgaaagtg
     4141 gttgatcagc aaaacgctgg cgatccggct tatnnnnnnn nnncttatcg tccaatggtg
     4201 gagaatttcg ctaactcgtg tgcttttaaa gctgccagcg atttgatctt cctcggcgtg
     4261 aaacagccaa acggctatac cgaaccgtta ttacacgcat ggcgtttacg cgaaaaagaa
     4321 aatcattaat agcgtcaaaa aaagccccgg caagatttat tgtcggggct atgatagtaa
     4381 ctgcggtagt acatttatcc cggaaataac cggagcgatt atcaggggtt aacaatatta
     4441 aatacccgta gcgcatcact agcgggatta aaacaacaat atggacatct taacgatact
     4501 ctgcgcctgc gtgtgcggaa cagaaaccct tacccgatat acttgttatg ccagaggtag
     4561 caattgcgtt agcggcgtac tgttgctgta aagtataacg cctatatttt acagttcagt
     4621 cctcttttgc ttattacaac cttaagtata tcaagcatat aaagataata agagactgaa
     4681 caatatggtt acctggaccc aaatgtatat gccgatggga ggactggggc tatccgctct
     4741 ggtcgccctg atcccaataa tattcttctt cgttgcactc gcggtattac gtctgaaagg
     4801 acatgtcgct ggagcaataa cccttatatt atctattctg attgcgatat tcgcctttaa
     4861 aatgccgatt gatatggcat ttgctgctgc gggctatggc tttatttatg gtttatggcc
     4921 tattgcgtgg attattgtcg cggcggtatt cctgtataaa ttaaccgttg ccagcggcca
     4981 gttcgatatt atccgcagct cggttatctc catcaccgac gatcagcgtt tgcaggtgtt
     5041 actgattggt ttctcctttg gtgcgctgct ggaaggggcg gctggctttg gcgcaccggt
     5101 ggcgattacc ggtgctctac tggtgggcct gggcttcaaa ccgctatacg cggcggggct
     5161 gtgtctgatt gccaacactg cgccggtggc gtttggtgcg ttgggtgtgc caattctggt
     5221 cgccggtcag gtaacgggaa tcgatccgtt cgacattggc gcaatggcgg gacgtcagtt
     5281 accgttcctg tcggttcttg tgccgttctg gctggtagca atgatggacg gctggaaagg
     5341 ggtgaaagag acgtggccag cggcgctggt tgcaggggga agcttcgctg tcactcagtt
     5401 ctttacctct aactatattg gtccggaact gccggatatt acttcggcgc tggtgagtat
     5461 cgtctcactt tctttatttc ttaaagtgtg gcggccaaaa aataccgaaa cggcaatcag
     5521 tatgggacaa tccgcaggtg cgatggtggt gaataaaccg tcttctggcg gtcccgtgcc
     5581 ttcagaatat cgtctggggc aaatcattcg tgcgtggtca ccgtttttaa tcttaacggt
     5641 gttggtcacc atctggacca tgaagccgtt taaagcgtta tttgctccgg gcggcgcgtt
     5701 ttattcactg gtgattaatt tccagatccc tcatttgcat caacaagtct tgaaagcggc
     5761 ccccattgtc gcccagccga cgccaatgga cgcggtgttt aaattcgacc ccctctctgc
     5821 tggtggcacc gctattttta ttgcggcgat tatctcgatc ttcatcctcg gcgtggggat
     5881 caagaaaggt attggcgtct ttgccgaaac gctaattagt ctgaagtggc cgatactgtc
     5941 gattggcatg gtgctggcat ttgccttcgt gaccaactat tccggcatgt ccaccacgtt
     6001 ggcgctggta ctggctggta caggcgtgat gttcccattc ttctcaccgt tcctcggctg
     6061 gctgggcgta ttccttaccg gctctgacac ttcttcaaac gccctgtttg gttcactgca
     6121 atcgaccacg gcgcagcaaa tcaacgtctc tggcaccctg ctggtggcag caaacaccag
     6181 cggcggagta actggcaaga tgatctcccc gcaatctatc gccgtggcct gcgccgcgac
     6241 gggcatggtg ggtcgagaat ctgaactgtt ccgctacacc gtgaagcaca gtctgatttt
     6301 tgccagcgtt atcggcatta tcacactgtt gcaggcgtat gtgtttaccg ggatgttagt
     6361 ttcgtaagtc aataaagccg gatgatcgtt aatatcatcc ggcttcgttt acattattct
     6421 gtcttattaa tggaaaatta tttcctgctt tattatccat gcggcaataa tgttttgcgt
     6481 tcagataaca tgctaaataa ttaagagcgt gagaacatct attttttatc acgcaaaaca
     6541 agt
//
LOCUS       DAWDRC010000169         6472 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5552_length_6472_cov_2.311516, whole genome shotgun sequence.
ACCESSION   DAWDRC010000169 DAWDRC010000000
VERSION     DAWDRC010000169.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5552_length_6472_cov_2.311516"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1618 a         1529 c         1639 g         1686 t
ORIGIN      
        1 tatcgagaac gaattattac gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt
       61 caccccacgt tgtcgacaac tgttagagga atatgagcag cagggcggtt ttaacgaaac
      121 acaggcacag gagtttgtgc aggaagcctt ggaaacgttt cgctggcacc agtcagcaac
      181 ggtagatgaa gaaacctatc gcgccttgca caacgaacat cggttgattg ctgatgtggt
      241 ctgttttcct ggatgccata tcaaccatct gacgccacgt acgctggata ttgaccgggt
      301 gcagtcgatg atgcctgaat gcggaattga acccaaagtt ctgatcgaag ggccgccgcg
      361 ccgcgaggtg ccgattttac tacgccagac cagctttaaa gcactggaag agacggtgtt
      421 gtttgcgggg cagaaacagg gcacacatac cgcgcgcttt ggtgaaattg agcagcgtgg
      481 tgtggcatta acgccgaaag ggcgacaact gtatgatgat cttctgcgga gcgctggaac
      541 cgggcaggat aatctcactc accaaatgca tttacaggaa accttccgca cttttcctga
      601 cagtgagttt ttaatgcgtc agcaagggct gggatggttc cggtaccgtc tgacgccttc
      661 gggtgaggcg catcgtcagg cgattcatcc cggagacgat ccacagccct taatagaacg
      721 tggttgggta gtggcgcaac ccattaccta tgaagatttc ttgcccgtta gcgcggcggg
      781 gatcttccag tcaaatctgg gtaatgaaac gcaggcatgc aatcacggtg atgccagtcg
      841 cgaagcattt gagcaggcgt tggggggtcc ggttttggat gagttccagc tttatcagga
      901 agcggaagaa cgcagtaaac gtcgctgtgg tttgctttaa attctgacca tccgcctttg
      961 caaaaatttg cctgatttta caaacgaatc aggctcatcc catcgacata aaaaaaatgc
     1021 caatttatgc atattctctg atttcaacaa ttggattatt aataaatatt gtctagagtg
     1081 agcggtcata aataagcact ttcttgccgc tgaaaatgat cagcgcggga ccatttacaa
     1141 caccagaagg actcactttc aggtatggat cgtagacgat ttattaaagg ttcaatggct
     1201 atggccgccg tgtgcggtac cagcggcatt gcttctcttt tttctcaggc ggcattcgcg
     1261 gccgattctg atattgccga cgggcaaacc cagcgttttg acttctccat tctacagtca
     1321 atggcgcacg acttagcgca aacagcatgg cgtggtgcac cacgtccgtt acctgacact
     1381 ttggcgacaa tgacgccgca ggcttataac agtattcaat acgacgccga aaaatcgctc
     1441 tggcataacg ttgaaaaccg tcaactggac gctcagtttt tccatatggg aatgggattc
     1501 cgtcgccgcg ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg tgaaattcac
     1561 tttcgcccag agttgttcaa atacaacgat gcgggtgtgg ataccaaaca attagaaggg
     1621 caaagcgatc tcggttttgc cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt
     1681 gatgtagtat cattcctcgg cgcgagttat ttccgcgccg ttgatgacac atatcaatac
     1741 ggtttgtcgg cccgcggcct ggcgatcgac acttacaccg acagcaaaga agagttcccc
     1801 gactttaccg ccttctggtt tgatacggta aaaccagggg caaccacctt taccgtttat
     1861 gcgttgctcg atagcgccag cattactggt gcctataagt tcactatcca ttgtgagaaa
     1921 aatcaggtga ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg
     1981 ggcattgcac cgatgaccag tatgttcagc tgcggtacta atgaacgtcg gatgtgcgac
     2041 accattcatc cgcaaatcca tgactctgat cgtctgtcca tgtggcgggg caacggcgag
     2101 tggatttgcc gtccgctgaa caatccgcaa aaattgcagt tcaatgctta caccgacaac
     2161 aacccgaaag ggtttggttt attgcaactg gatcgtgatt tctcccatta tcaggacatt
     2221 atgggctggt ataacaaacg cccaagtctg tgggtggaac cgcgtaacaa gtggggtaag
     2281 ggcaccatcg gcctgatgga aatcccaaca acgggcgaaa cgctggataa cattgtctgc
     2341 ttctggcagc cagaaaaagc tgtaaaagcg ggtgatgagt ttgcattcca gtatcgtctg
     2401 tactggagtg cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc gacgcgtacc
     2461 ggcatgggcg gtttcccgga aggttgggcg ccaggtgaac actatcccga aaaatgggcg
     2521 cgacgttttg ccgtcgattt cgttggtggc gatctgaaag ctgccgcgcc aaaaggcatt
     2581 gagccggtga ttacgctttc cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa
     2641 cccattgatg gttatcgtat tcagtttgac tggtatccga cttcggactc cactgatccg
     2701 gtcgatatgc ggatgtatct gcgttgtcag ggcgacgcta tcagtgaaac atggctgtat
     2761 cagtatttcc cgccagcgcc ggataaacgt cagtatgttg atgaccgcgt gatgagttaa
     2821 tcgttttttc ttcggcatct tcttcgggag gtgccgtctg gttaaacacg atcccgctcg
     2881 catttttccc taagttaaat gagtaatctg atggtgtgta tttcagatac accttgtcag
     2941 ccactaacag ggagtgcgta tgtttccaga ataccgagat ttaatatccc gtctgaaaaa
     3001 cgaaaatcct cgctttatgt ccttgttcga taaacacaat aaacttgatc atgaaattgc
     3061 cagaaaggaa ggttccgacg gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca
     3121 aaagctacag ttaaaagatg agatgctcaa aatcctgcag caggtgagcg tcaaagaggt
     3181 gtaaactttc ctaagccgcc agccaggcgg cttttttaac agctgcatgg attgactgga
     3241 gataagatga ctgaaacgat aaaagtaagc gaatcacttg aattacatgc tgttgcggaa
     3301 agtcatgtca cacctcttta tcagttaatc tgtaaaaata aaacctggtt acagcagtcg
     3361 ctaaactggc cgcagtttgt tcaaagtgaa gaggacacgc gaaaaacggt gcagggtaat
     3421 gtgatgttgc atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga agatgaactt
     3481 atcggcgtta tctcttttaa tcgtattgaa ccactgaata aaaccgctga aatcggctac
     3541 tggctcgacg aatctcatca ggggcagggg atcatttctc aggcgctgca ggcattgatt
     3601 catcattacg ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat
     3661 gcgcaaagca accaggtcgc tttgcgcaat ggttttatcc ttgaaggttg cctgaaacag
     3721 gctgagttcc tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat tatcgattca
     3781 caataaccct gccaacggcg ttcgagtaat gcgctcctcg ccgttaatga cttttggacc
     3841 acggacatga atcgtgtcgc catcaaaggc ttcaacgacg gcgtggtcgg ttaactccac
     3901 atggttttca attatcaccg caccagtaat acggctctca ccttgtatga caacgtgctc
     3961 gtcgagcaga atcggtccgc cacgtaccac ggcatttccg ccaatcaaga catgatgttt
     4021 taacacacaa ttaccttcca caatggcata ttccgccacc tgtgaactgt aatgaatcgt
     4081 cggaatggca tcttcttcta tgccagcttt cacctgtgcg tgaccgtaaa ctttagcgca
     4141 atcgcatagc cagacgttgt tctcttcatt accttcaata ctggcaaaat caaaaacttc
     4201 ggcgcgatgt tcaataaaag cataccggac gacggcatcg ccataaattt gtgcctggtg
     4261 gacaatacgc gaggcgctaa ccctcgcgcg atcataaatt tgtagcagga gttgatggtc
     4321 gggcgttaag ccttgtgcgg cgacgatcat agaatgttga tcaattaagg cgtgaccaaa
     4381 tattctacat tgaccgcaaa ctaatgagtc actaatggtg acgctgtcac ttatatatgc
     4441 accctggttg atttcactat tatcgatcca gacattatcc gttgcgtaaa cctctcccca
     4501 cagcacgctg gtgccggtga tacgcgtatt cccggaaatc accgttccgc cgaacgcaat
     4561 agcattctgg tcgtaaatcc agcaatttcc ttcctgtgct aacaccgttt cgcgatcgat
     4621 ccagccgcca gcgcttcctg ctatcacatc gttaaaatcg ctcatggcga taatttgccg
     4681 taataacaca ctttttttag tgccatcttc ctgataacta aaggctcgct gttcttcact
     4741 tagacgatat ttacgcatag cgttttccca caggtgactt actataaccg tagcaaattc
     4801 tgcggctctg gctatgctcg agaaaattcc ataaaatgca tttcaaatat actttataaa
     4861 ttaaacaaaa tgagtaagaa gatgcagagc gataaagtgc tcaatttgcc ggcaggctac
     4921 tttggtattg tgttggggac gatagggatg ggatttgcct ggcgctatgc cagccaggtt
     4981 tggcaggtca gccactggtt gggggatggg ctggtgattc tggcgatgat catctgggga
     5041 ttattgactg gcgcatttat tacccgactc atacgctttc cgcatagcgt gctggcagaa
     5101 gtgcgccatc cagtgctgag cagttttgtg agtttgtttc cggcaacgac gatgctggtg
     5161 gcgattggtt ttgttccgtg gtttcgccca ctggcggtgt gcctgttcag ttttggtgtc
     5221 gtggttcagt tggcttatgc cgcctggcaa actgcgggat tatggcgcgg atctcaccct
     5281 gaagaagcta ccacgcctgg actgtatctg ccgacagttg ccaacaactt tatcagcgca
     5341 atggcctgtg gtgcgttggg ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt
     5401 ttctcatggc taagcctgga accggtgatc ttgcagcgtc tgcgcagttc gggagaatta
     5461 cccacggcac tgcggacatc actcggcatt cagctcgctc ctgcgctggt ggcctgtagt
     5521 gcctggctga gcgtcaacgg cggcgagggt gacacgctgg cgaaaatgct tttcggttat
     5581 ggactgctgc aactgctgtt tatgctacgt ctgatgccat ggtatctctc ccagccattt
     5641 aatgcttcat tctggagttt ctcgttcggt gtatctgcac tggcaaccac cggtttgcat
     5701 ctggggagtg gcagcgataa tggatttttc catacgctgg cggtgccgct gtttatcttt
     5761 accaatttta ttattgcaat actgctcatc cgtacttttg cgcttctgat gcagggaaaa
     5821 ttgttagtca gaaccgagcg cgccgtttta atgaaagcag aggacaaaga atgatcattc
     5881 gtgatgaaaa ctattttact gataaatatg aattaacccg cacacactct gaagtgctgg
     5941 aagcggtgaa agtggttaaa ccgggtaaaa cgctggatct gggctgtggc aatggtcgta
     6001 acagtcttta cctggcagcc aatggttatg atgttgacgc atgggataaa aatgccatga
     6061 gtatcgccaa cgtcgagcgc attaagtcca ttgaaaatct ggataattta cacacccggg
     6121 tcgttgatct gaataacctc acatttgatg gacagtacga ttttattctt tcgactgtgg
     6181 tgctgatgtt ccttgaggct aaaaccatcc cagggctgat tgccaatatg caacgttgca
     6241 ctaaacctgg cggttacaac ctgattgtgg cggcgatgga taccgctgat tatccatgta
     6301 ccgtcggctt cccgtttgcc ttcaaagagg gggaattacg tcgatattac gaaggctggg
     6361 agagggtgaa atacaatgaa gacgtcggcg agctgcaccg caccgacgcc aacggtaatc
     6421 gtattaaact gcgtttcgcc acgatgctgg cacgtaaaaa atgacccggt aa
//
LOCUS       DAWDRC010000170         6466 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5558_length_6466_cov_2.087818, whole genome shotgun sequence.
ACCESSION   DAWDRC010000170 DAWDRC010000000
VERSION     DAWDRC010000170.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5558_length_6466_cov_2.087818"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    686..695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1096..1105
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5553..5562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1483 a         1764 c         1581 g         1608 t
ORIGIN      
        1 ttgtttcccc tgcgctttcg accagcaccg aaccggtatc aacgcgcgag acggagtctt
       61 caatgagggc tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt
      121 cgcccgccac cacggcaaaa ccacggcctt gttcacccgc tcgcgcggct tcaacagcgg
      181 cattcagcgc gagtatatta gtctggaagg caataccatc gataacgctg ataatgtcgg
      241 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
      301 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
      361 tatcggcgtt ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca
      421 gcgcggatgc ctgctgttca gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
      481 gggtaccggc atagatggca tctgaacctt cgcgcacatg agtgacggtg tcagtcaaag
      541 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
      601 tcagggtatt cgccaggtta ccaccggcaa tttcgcgaat atgcgcaata atttttgcca
      661 gcggtgtaag caacatacgg cgaatnnnnn nnnnnaccag cagaatcaat accaccacca
      721 gcgcgataac cgccagttgc cactgggcaa atcggtaatc atctgcgttg tcagtgacga
      781 tatcgcgata cagtttttca ctgctgaggg cgtactgagc aaacgcttcg cccattgcat
      841 tttgcattcc ctgggttggc tgagcgaaat aagctccagt attgccataa tcaagataat
      901 caatcagttc agttaacgct gtgtgatagt ttttatattt ttcatcaata ttacgactgg
      961 tagcgaccat ttcaggtaac ggtgccatgc ttttgaattt tttataatga gtcgcggcct
     1021 gggccaatgt tttcctggcg ctatcgagca attcaacttt ggcattactt tgctgattgg
     1081 atgaatccat catcannnnn nnnnntccgc accgctgaac gactcaggtt aatgcgcgtt
     1141 tgcagcatta aatcccatgt tgacgtcagc tcgccctgct gttcccgtaa ttgattggaa
     1201 accacaaagc tcttctggct atggtgaagg gaagaaaaaa acagactgcc ggaaataagc
     1261 tgtaacagtg cgaatacccc cagcaccatt accaacagcg tgactacgcg gatacggtta
     1321 atcataaggc accttcctga aaacaagttg atctcgttat cggcaaggag gggggaaact
     1381 ttattgctga tgccacccgc cgcgaaattg aaataaaaaa cccgatgcgc agatcatcgg
     1441 gttcatttca attgaggaaa tcgggagaat tacgccactt ctgacgccgc gctatctaac
     1501 agcgccatct cttcgctgtt cagcagtttt tcgatgttca ccagaatcaa catgcggtcg
     1561 cccagtgcgc ccagtcctgt gagatattcg gttgaaagcg tcacggcaaa ttccggtgcc
     1621 ggacgaattt gctccgccgt caatgaaagc acgtctgaga cgccgtcaac cacgatgccg
     1681 accacccgct gtccgagatt caggacgata actaccgtgt tgtcgttata gtccacatcc
     1741 acctggctga acttaattcg taagtcaaca atcggcacaa taacgccgcg cagattcgtg
     1801 acgcctttga taaacgctgg cgtgttcgca atccgtgtca cctgatcgta gccacggatc
     1861 tcctgcactt tcagaatatc aataccgtac tcttcatcac caagggtaaa taccagaaat
     1921 tcctggcctg acggctcgct ggccagcttt gttacattcg tcataccggt catattgtta
     1981 cctttttact cattcaggcg gcggtgttcg ccatacgttg ttcgcggttt atcgcctgca
     2041 aggcggagac atcaacaatc agtgccacgc tgccgtcgcc aagaatggtc gcagcagaaa
     2101 tgccggggac tttgcgatag ttactttcaa ggtttttaac cacaacctgg tgttgaccaa
     2161 ttaattgatc caccagcaag gcgtagcggc gaccgccact ttgtaagatc accacaattc
     2221 cctgggtggc ttcggttttc gcgcccgcga cgttgaacac tttccacagt tcgacgatgg
     2281 gcagatattc accccgcact tccagcaccc gctcgccgcc agccagtgga tgcagatcgg
     2341 cttcacgggg ttgcagtgat tccatgacag cattcagcgg cagaatgaaa acttcatccg
     2401 caacgcgtac ggacatgccg tcaagaattg ccagcgtcag cggcagtaaa atgcggatcg
     2461 tcgtgccagt accctgcttc gactggatct cgacatgacc gcccatctcc tggatattac
     2521 gtttaacgac gtccatgccg acgccacgcc cggagacgtc ggtgacctgc tccgccgtgg
     2581 agaagccagg tgcaaatatc agcattgcaa cttcgtcgtc gctcatgttt tcgctgacgg
     2641 tcagaccttg cgaggccgct tttgccagaa ttcgttcacg gtttagcccc gccccatcgt
     2701 cggtcacttc aatgcaaatg ttgccgccct gatgttcggc agacagaatt aaatttccga
     2761 cgctgttttt acctgcagcg agccgttttt ctggcagttc aataccgtga tcgaggctat
     2821 tgcgtaccag gtgggtcagc gggtcgataa tgcgttctat caggctcttg tcgagttcag
     2881 tggaactgcc caccagcgtc agttctacct gcttgccgag ttttcccgcc agatcacgca
     2941 ccagccgggg atagcgacta aagacatatt ccatcggcat catgcgaatc gacatcactg
     3001 attcctgcaa atcacgagcg ttacgttgta actgccccat gctggtaatc aaatcaccat
     3061 gattaaccgg gtccagttca ctggagcgct gagcaagcat ggactgggtg atgaccagct
     3121 cgccgacgag gttaattaat tgatcaacct tttctaccgc tacacggatg ctggtggatt
     3181 cactgctgcg cgtcgttttt tcccgctcca cgcgaccggt tggcgcttgt tcggctgcca
     3241 gtttaagcac tggtggggtg gatatttttg gcgagacgtc tactgtttca aaggtaatct
     3301 gatcggcttc aatcacaaaa cagagtaccg ctgtgatgtc atcttcggcg atgtcgcccg
     3361 gtaatattgc cgagagagaa tccgcccctt tcaccacgtc agttaacgtt gtcagatgcc
     3421 ccaactcttc ttccagcagg tcgacttcgc cggccttcag gcgcgaaagg ataattcgtc
     3481 gcggtgactg actacgactc tgctcatctt gcggttcact tttggcaacc acacttaatc
     3541 gggtcactgc agatggcgtt tcgcctttcg cttctaatgc cagttgacgc aaggcctggc
     3601 agatataatc gaagctggcg gcatccggct cttgcgactg tttataagcg tcgagctgtt
     3661 cttgcatgat gtccttcgtt tccaaaaaca gattgataat gtcggtgttg agttgcatct
     3721 cacctcgtct ggcttcatcg agcaggtttt ccatcagatg cgtggtttcc tgcaaaacgc
     3781 tgaagccaaa ggttcctgcc cctcctttga tcgagtgggc agcccgaaag atggcattca
     3841 actgttcggc atctggcgct tccggctgca aaaccagcag atgctgctcc atatcagcca
     3901 acagttcgtc cgcttcatca aaaaatgtct gataaaaatc gcttatatcc atgctcacgc
     3961 tgtcacctcg gttcggctga tggcattgtg ggaacactga cctgtggtgc aacctcaggt
     4021 ttttccaggg cgcttactgg ctcattctgg ctttcggcgt tttcatgcaa aatggcctgt
     4081 tcggcttgtt tgttcagtac cagcaggctg atgcgacggt tgacggcatc atcaggtccg
     4141 cgatcgctta agcgcatcgt tgccgccatg ccgacgacac gtaacacttt gccgccattc
     4201 aacccaccaa ccatcagttc gcggcgggat gcattggcac gatcggcaga aagctcccag
     4261 ttgctgtaac ctttctcacc actggcgtag ggaaaatcat cggtatgacc cgagaggcta
     4321 attcggttgg gaataccgtt cagtacgggc gcgatggcgc gcagaatgtc gcgcatatag
     4381 ggttcgacgt cggcactgcc agttctaaac atcgggcgat tctggctatc gatgatctga
     4441 atacgtagac cttcctggac cggatcgatt ttgagatgtg gacgtaacgc ccgcagtttc
     4501 ggatcggact caatcaactg gtcgagatcg ccacgcaatt tccgcaatcg actttgctcc
     4561 atgcgttttt tcagctcttc gatgttcggc tgcttattca cttccccctg gctttgggtg
     4621 taatcatcac ccccaccggg aattgggctt tcactattag aaatgcgatc gccgcccgta
     4681 accgcagtcg ccagtggagt ccggaagtac tccgcaatct gaatcagctc ttttggactg
     4741 gagatagaga tcagccacat caccagaaaa aaggccatca ttgcggtcat aaagtcggca
     4801 taagcaatct tccacgatcc atgtgctgct ccgtggcttt tggctttgcg tcgtttgacg
     4861 acaataatcg gatgcgcttg attcttcatg cttcctcggt tgtcgtctgt tgttgcggat
     4921 ttttcaccgc acgcacatgc tcttccagct caataaacga cggacgttcg ctggaataga
     4981 gcgttttgcg accaaactca acggcgatag gcggtgcgta accgttcaga ttagatagca
     5041 gtgtgacttt gacgcactgc atcattttgc tggtttcggc gcttttctga cgtaaaacac
     5101 tcgctaatgg ggaaataaat ccgtaagcca ataaaatacc gaggaaagtc cccaccatcg
     5161 catgtgcgat aagcgccccc aactcggcgg caggacgatc ggctgaacct aacgcgtgaa
     5221 cgacccccat tacagccgca acaataccaa acgccggaag tgagtccccg accagcgcca
     5281 gactgttcgc cgggacttct gcctcgcttt cgtgcgtctc aatctcttca tccatcagag
     5341 cttcgatttc gaaggtgttc atgtgaccgc tgataatcag gcgcagataa tcgacgataa
     5401 attcaagcat gacgctatcc gcaaggatgc gtgggtagct ggcgaagatc tcgctctcac
     5461 ggggattttc aatatcgcgt tccagcgaaa acatccccat ctgccgcgat ttcgccatca
     5521 accgataaag cagagccagc agatccatat acnnnnnnnn nnttgctttg gtgtatttgg
     5581 agcgacgaaa cagcaacggc agcgccttca gcgtgccttt aatcgctttg ccattattgc
     5641 cgacgataaa cgacccaatc cctgcaccgg caataatcac cagttcagcg ggttgataga
     5701 gtgctccaag gcttccaccg gtcatcaaat aaccgccgaa aactgtaccg agaacaacca
     5761 ggtaacctaa taagataagc acgacatcat ccttccactg ttgaccatga caggatgttc
     5821 agtcgtcagg cgttaacacg cgataggggc aaaaaaaagc agcggtacgt cgttaccgcc
     5881 gctggaatgt ttcgccacac cgtatcagtt aaacagcctg tactctctgt tcatccagca
     5941 gttgtgggat aatatcggca ggattctggg aaagtttacg tctttttact gcccgggatg
     6001 gcggctgaca taagctgcag gcaaagctgc caacaggttg gtgagcgtgg gtaataaaat
     6061 tgccgccgca gcagttgcag ctggaaagtt gcagtaatcc actttcaaca aaccgcacca
     6121 atgtccaggc acgggttaat gccagcagcg gtccttcttc tgcttgcggg cactgttcaa
     6181 ggtataaacg gtaggctttg atcaccgcat cgacgccatt acacaagcca gttttcagta
     6241 aaaactgcca tgcattacag aacatcgaag catgaacatt ttgttcccag gtcataaacc
     6301 agtcggttga aaatggcagc atgcctttcg gcggtgggct tccgcgcagt tctttataga
     6361 gttttatcag acgtccgcga cttaactgtg tttcgctttc cagcatctgc aaacgagcgc
     6421 ccagggtgat caattccatt gccagctgaa tatcccgcgc ttcctg
//
LOCUS       DAWDRC010000171         6448 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5573_length_6448_cov_2.434851, whole genome shotgun sequence.
ACCESSION   DAWDRC010000171 DAWDRC010000000
VERSION     DAWDRC010000171.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5573_length_6448_cov_2.434851"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    6232..6241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1723 a         1550 c         1464 g         1701 t
ORIGIN      
        1 gcatcataaa gaacgaaata gtaataagaa taaatagcgt cggaatcgct tccagacagc
       61 gacgtagaat aaattttaac attgccctac cttctggcct gtgccttctg taatgcgata
      121 aaaatcagac accgtggagc aggacactcc tgccccacgt attgccatta gtgcttcaca
      181 atgtacatat tccgggtgta ggtattatcc agcggatctt tgccggtata gccaccaacc
      241 cacggtttca ccaaacgcgc attcacgtag taataaacag gaacaatggc cgaatcctta
      301 tccagctgtt gttctgcttt agtgtacaga gctgtacgct gcgcctcgtc agtcactttc
      361 agcgtttccg ccataatgct gtcaaaggcc gggctcttat aatgcgcggt attcattgag
      421 ctattcgaca gcatggtgtt caggaaggaa gttggttcgt tgtagtctgc acaccagcct
      481 gcacgggcca catcaaaagt gccctggtga cgggtgtcga ggaacgtttt ccactcctgg
      541 ttaaccagtt tgacgtttac accaatgttt ttcttccaca atgaagaggc agcaatcgcc
      601 agctttttat gcagatcgga ggtgttatac aacaggttga tggtcaacgg tttgtctgcg
      661 gtataacccg cttcagccag cagttttttc gcttcttcgt tacgtttttc ctggctccag
      721 ccaaaccatt ccggctgagt caattttgcg ccatcagtat atggtggagt gtaaccatag
      781 gcgggcatgt cgccctgcgc tttcacttta ttaacaatga tatcgcggtc catacccaat
      841 ttcagtgcgg tacgcacacg cacgtcatta aatggcggtt tctggttgtt aatttcgtaa
      901 tagtaagtgc acaggtatgg atcaacgtga acttcgtccg ggatctcttt tttcagcttc
      961 tggaacaatt cgatcggcat gttgttataa gtcatgtcga tttcaccact acggtagcgg
     1021 ttgacatcgg taacttcaga agcaataggc aaataggtta cctggttaat aacggttttc
     1081 gcgttgttcc agtaggtcgg gctgcgttca agaacgattc gttcgtttac gacccaatct
     1141 tttaaggtat aggcaccgtt ggtgacgata ttaccaggct gggtccattt ttcgccgaat
     1201 ttctcgatag cggcttttgg caccggtgaa gttgatgggt gaacaagtaa tttatagaag
     1261 tacggaacag gttcacttaa ggtgacttct aatgtgtgat catcaatagc tttcacgccg
     1321 agatcggtaa tcggtttttt cccttcaaga atttcatcaa taccggcgat atgcccatat
     1381 tgcagataac tggcatacgg agaagcagtg tttggatcaa cagaacgttg ccagctatac
     1441 acaaagtctt gtgctgtaac tggcgtgcca tcagaccatt tcgcatcttt acgcaaatgg
     1501 aaggtccaga ctttcgcgtc tttattatcc catgattcag cgacgccagg tgccggatga
     1561 ccgtcaagat cgctgaccag taagccttca aacaggtctc ggctgatatt agactccgga
     1621 accccttcaa ttttgtgcgg atctaatgac tgaacttctg aaccattgtt acgtaccagc
     1681 gtttgttttt ccgccagtgt gacacctgcg ggtacatcag ctgccagcgc gacattccct
     1741 gccataagcg cagccaaaac gccagctgct actaaacttc tcttggtgat gttggtcatt
     1801 gttttttgga ctccctcatt ataattactg gtattaacca gcatgtgtaa tcccggattg
     1861 ggaccctgca caggctcgga gactttctgc tgtcaggtta ctttatcgtt acgtattact
     1921 atcaccgact ttatttattc tggtgattca gacgatcacc ttatgtcgat tcgtactgtg
     1981 tctcccctgt cgagacacat tattctttcg cgacgatctt taattgttaa ttattctcat
     2041 taccaataaa gaatcgcctg gcggttgccc ggaaagtacc aaatgcggtt cttactcgcc
     2101 aatacatttt gcaaaattgt taagcaattc tcttttattg accgtatcag aatctatagc
     2161 ttgcatccat gccttaacaa acatctttca attcaatcaa atagaggttg gttaagcatg
     2221 tctctctttt ctgattacag ttccagtagt gaaatgcaca ataatttaac aattgattat
     2281 tatttagcac tttcctctac caaaggcagt ggaattacta atatcatttc aattattctt
     2341 cagcaagccc aggattatga tgtggcgaaa ataacatgat ggtgaggaga agcagttaag
     2401 gaaatcccta taactaactg taatttgaga agaatatttt tacctattcc ttttgctttt
     2461 gataactgcg caggaacaaa gtaaaaataa tgacgaagta aacggagcat ggcagctccg
     2521 tttcatttga aaggaattaa ttaagcaggc cggggaaaat ccccttaata ccagtaacga
     2581 taaattcaat ccccaacgcc atcagcaata gccccataat acgcgtaatc acgttgatgc
     2641 cggtctggcg taaaactcgt accagccacg gtgccatgcg gaacaatccc cagcaacata
     2701 aagcgaacaa tgcaatagcc acaaagaaac caaacagata gctaatgctg tgataacgcg
     2761 taccccagac gatggtagaa ctgatcgccc ctggccccgc catcaacggc aacgccaatg
     2821 gcaccacacc aatgctttca cgcaccgcgg tttctgattt ttcttgcttg ttctgtttat
     2881 cctcaccaag cttgccgctg atcatcgaca tcgctattgt caccaccagg atgccaccgg
     2941 cgatacggaa tgaatcaatt gatataccaa aaagttgtag aatcgtgtcg ccgagaaaaa
     3001 gcgagatcca caagataatg gccacagaca ggttggctgt aaggttagtt ttgtttcgcg
     3061 ctgccgctgt ctgataactg gtcatgctga taaagacggg aataatccct accgggttga
     3121 ccagcgcaaa taacccgatg aaaaatttga agtaaacggg aaaatcaaaa agggtctgaa
     3181 tcacggttag ctccgaagca aaagccggat aatgttagcc ataaataagg ttgaaaagac
     3241 gcgctgacaa tacgcctttt gacagcattt ttcacctcct aactacttaa aattgctatc
     3301 attcgttatt gttatctagt tgtgcaaaac atgctaatgt agccaacaaa tcatactaca
     3361 atttattaac tgttagctat aatggcgaaa agccatgctg aaaggtgtca gctttgcaaa
     3421 attttgattt ggatcacgta atcagtaccc agaagtgagt aatcttgctt acgccacctg
     3481 gaagtgacgc attagagata ataactctaa tgtttaaact cttttagtaa atcacagtga
     3541 gtgtgagcgc gagtaagctt ttgatttcca tcggttaagc aaatcatcac cgcactgact
     3601 atactctcgt attcgagcag atgatttact aaaaaagttt aacattatca ggagagcatt
     3661 atggctgtta ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa aaaagcccag
     3721 cgtgaatatg ccagtttcac tcaagagcaa gtagacaaaa tcttccgcgc cgccgctctg
     3781 gctgctgcag atgctcgaat cccactcgcg aaaatggccg ttgccgaatc cggcatgggt
     3841 atcgtcgaag ataaagtgat caaaaaccac tttgcttctg aatatattta caacgcctat
     3901 aaagatgaaa aaacctgtgg tgttctgtct gaagacgaca cttttggtac catcactatc
     3961 gctgaaccca tcggtattat ttgcggtatc gttccgacca ctaacccgac ttcaactgct
     4021 atcttcaaat cgctgatcag cctgaagacc cgtaacgcca ttatcttctc cccgcacccg
     4081 cgtgcaaaag atgcaaccaa caaagcggct gatatcgttc tacaggctgc tatcgctgcc
     4141 ggtgctccga aagatctgat cggctggatc gatcaacctt ctgttgagct gtctaacgca
     4201 ctgatgcacc acccagacat caacctgatc ctcgcgactg gtggtccggg catggttaaa
     4261 gccgcataca gctccggtaa accagctatc ggcgtaggcg cgggcaacac tccggttgtt
     4321 atcgatgaaa ctgctgatat caaacgtgca gttgcatctg tactgatgtc caaaaccttc
     4381 gacaacggtg taatctgtgc ttctgaacag tctgttgttg ttgttgactc tgtttatgac
     4441 gcagtacgtg aacgtttcgc aacccacggt ggctatctgt tgcagggtaa agagctgaaa
     4501 gctgttcagg acgttatcct gaaaaacggt gcgctgaacg cggctatcgt tggtcagcca
     4561 gcctataaaa ttgctgaact ggcaggcttc tctgtaccag aaaacaccaa gattctgatc
     4621 ggtgaagtga ccgttgttga tgaaagcgaa ccgttcgcac atgaaaaact gtccccgact
     4681 ctggcaatgt accgtgctaa agatttcgaa gacgcggtag aaaaagcaga gaaactggtt
     4741 gctatgggcg gtatcggtca tacctcttgc ctgtacactg accaggataa ccaaccggct
     4801 cgcgtttctt acttcggtca gaaaatgaaa acggctcgta tcctgattaa caccccggct
     4861 tctcagggtg gtatcggtga cctgtataac ttcaaactcg caccttccct gactctgggt
     4921 tgtggttcct ggggtggtaa ctccatctct gaaaacgttg gtccgaaaca cctgatcaac
     4981 aagaaaaccg ttgctaagcg agctgaaaac atgttgtggc acaaacttcc gaaatctatc
     5041 tacttccgcc gtggctccct gccaatcgcg ctggatgaag tgattactga tggccacaaa
     5101 cgtgcgctca tcgtgactga ccgcttcctg ttcaacaatg gctatgctga tcagatcact
     5161 tccgtattga aagcagcagg cgttgaaact gaagtcttct tcgaagtaga agctgacccg
     5221 accctgagca tcgttcgtaa aggtgcagaa ctggcaaact ccttcaaacc agacgtgatt
     5281 atcgcgctgg gtggtggttc cccgatggac gctgcgaaga tcatgtgggt tatgtacgaa
     5341 catccggaaa ctcacttcga agaactggcg ctgcgcttta tggatatccg taaacgtatc
     5401 tacaagttcc cgaaaatggg tgtgaaagcg aaaatgatcg ctgtcaccac cacttctggt
     5461 acaggttctg aagtcactcc gtttgcggtt gtaactgacg acactactgg tcagaaatat
     5521 ccgctggcag actatgcact gaccccggat atggcgattg tcgacgccaa cctggttatg
     5581 gacatgccga agtccctgtg tgctttcggt ggtctggacg cagtaactca cgccatggaa
     5641 gcttatgttt ctgtactggc atctgagttc tctgatggtc aggctctgca ggcactgaaa
     5701 ctgctgaaag aatatctgcc agcgtcctac cacgaagggt ctaaaaatcc ggtagcgcgt
     5761 gaacgtgttc acagtgcagc gactatcgcg ggtatcgcgt ttgcgaacgc cttcctgggt
     5821 gtatgtcact caatggcgca caaactgggt tcccagttcc atattccgca cggtctggca
     5881 aacgccctgc tgatttgtaa cgttattcgc tacaacgcga atgacaaccc gaccaagcag
     5941 actgcattca gccagtatga ccgtccgcag gctcgccgtc gttatgctga aattgctgac
     6001 cacctgggtc tgagcgcacc gggcgaccgt actgcagcta agatcgagaa actgctggca
     6061 tggctggaaa cgctgaaagc tgaactgggt attccgaaat ctatccgtga agctggcgtt
     6121 caggaagcag acttcctggc gaacgtggat aaactgtctg aagatgcatt cgatgaccag
     6181 tgcaccggcg ctaacccgcg ttacccgctg atctccgagc tgaaacagat tnnnnnnnnn
     6241 nattctgctg gatacctact acggtcgtga ttatgtagaa ggcgaaactg cagcgaagaa
     6301 agaagctgct ccggctaaag ctgagaaaaa agcgaaaaaa tccgcttaat cagtagcgct
     6361 gtctggcaac ataaacggcc ccagaagggg taatgccagt cagttaagga tcagttgacc
     6421 gatccagtgg ctgtgtaaga atccggaa
//
LOCUS       DAWDRC010000172         6422 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5596_length_6422_cov_2.581750, whole genome shotgun sequence.
ACCESSION   DAWDRC010000172 DAWDRC010000000
VERSION     DAWDRC010000172.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5596_length_6422_cov_2.581750"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1885..1894
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1508 a         1492 c         1764 g         1648 t
ORIGIN      
        1 acgataagct gaaagattta cgtgagcgca aagccagtct ggaagctcgt gcgctggaag
       61 cgttaagcaa gaacgtgaat ccgtcgttga ttaacgaagt tgctgaagaa atcgcgcgcc
      121 ttgagaatct cattaccgct gaagagcaag tgttgtcgaa tctggaagtc tctcgtgatg
      181 gcgtggaaaa agcagttaca gcgacagcgc agcgtatcgc tcagtttgag cagcaaatgg
      241 aagtcgttaa agccactgaa gccatgcagc gtgcacaaca ggctgtaacg acctctactg
      301 ttggcgcatc ttccagcgtt tcgacagcgg cagaatcctt aaaacgcctg caaacgcgtc
      361 aggccgaacg tcaggctcgc ctggatgctg ccgcacagtt ggaaaaagtc gcagacggtc
      421 gcgatctcga tgaaaagctg gcggaagccg gaattggcgg tagcaataaa agtagcgccc
      481 aggatgtatt agcaagactg caacgccaac agggcgagta attttttgcc agcctcactg
      541 caaggctggc aactatttta aggataaaat atgtctggtt ttttccagcg tctgtttggc
      601 aaggataata agcctgctat cgctcgcggt ccgttgggtc tgcatctcaa tagtggtttt
      661 acgctcgata cgttagcgtt tcgtttgctg gaagatgcat tgctgattgc gctgccgggt
      721 gaggaatata ccgtggccgc cgtcagttgc atcgatctgg gcgggggtag ccagattttc
      781 cgttactaca cttcgggcga tgagttcctg caaatcaata ccactggcgg cgaagatatt
      841 gatgacattg atgatatcaa gctctttgtc tatgaagaga gctacggtat cagtaaagag
      901 agtcactggc gcgaggcgat caacgccaaa gtgatggggg caatgacctt aaactggcag
      961 gaaaaacgct ggcagcgatt ttttaacagc gaagaaccgg gaaatatcga acccgtctac
     1021 atgctggaaa aagtagaaaa tcaaaaccat gccaaatggg aagtgcataa ttttaccatg
     1081 ggctatcaac ggcaagtgac cgaagatact tacgaatatc tgctgttaaa tggtgaggaa
     1141 tcttttaatg atctcggcga gccggagtgg ttattttcgc gcgcgctggg ggtcgatatc
     1201 ccgctgacat cacttcatat tattggttaa ttacaaggac gtttacgatg catatactgg
     1261 attcacttct tgcctttagc gcttattttt ttattggcgt ggcgatggtg attattttcc
     1321 tgtttatcta ctccaaaatt acaccgcaca acgaatggca gttaatcaaa aacaataaca
     1381 ccgcggcggc actggcgttc agcggaacat tgttggggta cgtgatcccc ttatccagtg
     1441 cggcaatcaa tgcggtgagt attccagact atttcgcatg gggcgggatt gctctggtga
     1501 ttcaattact cgtttttgct ggcgtcaggc tttatatgcc cgcattaagc gaaaaaatta
     1561 ttaatcacaa taccgcagcc ggaatgttta tgggaaccgc cgcgctggct ggcggtattt
     1621 ttaacgcagc ttgtatgaca tggtaatgga cgatcatggc cagaaaacgc aaatccagaa
     1681 ataacaaaaa tatcggacat ggggcaatca gtcgcattgg tagaccgcat aatccttttg
     1741 agccgcgccg caatcgctac gcacaaaaat acttaacgtt ggcactaatg ggcggtgccg
     1801 cttttttcgt attgaaaggt tgtggcgata gtggcgatgt cgataacgac ggcgacggaa
     1861 cgttttacgc gactgtgcag gattnnnnnn nnnnttgtat tgatgatggt aataattcag
     1921 atatctgtgc tcatggctgg aacaacgcca aagcggcgtt ttatgccgat gttccgaaga
     1981 atatgactca gcagaattgt cagtctaagt acgaaaattg ctactacgac aatgttgaac
     2041 agagttggat cccggtcgtt tctggatttt tgttaagccg ggttattcgt aaagatcgcg
     2101 atgagccgtt cgtctataac agcggcggat cctcatttgc ttcccgcccc gtctggcgca
     2161 acacttccgg tgattactcc tggcgctccg gttctggcaa aaaagagtct tactcttcgg
     2221 gcggctttac caccaaaaaa gcgtcaaccg tttctcgcgg cggctatggt cgttcttcca
     2281 gcgcccgtgg gcattgggga ggctaatcat gctgagacac aacgttcctg tgcggcggga
     2341 tctggaccag atcgccgccg acaacggttt cgactttcat atcatcgaca atgaaatcta
     2401 ttgggatgag agtcgggctt accgctttac tctgcgccag attgaagagc agatcgaaaa
     2461 accgactgcg gaactgcatc agatgtgcct ggaggtggtg gatcgcgcgg ttaaagatga
     2521 agagatcctg acgcaactgg cgatcccgcc gttgtactgg gatgtgatcg ccgaaagctg
     2581 gcgcgcacgc gatccttcgt tgtatggccg tatggatttt gcctggtgtg gtaatgcacc
     2641 ggttaagttg ctggagtaca acgccgatac gccaacttca ttgtacgagt cggcttattt
     2701 ccagtggttg tggctggaag atgcccggtg cagcggcatt attccgcgcg atgccgatca
     2761 gtacaatgcc attcaggagc gcctgatttc acgctttagc gagctttaca gtcgggaacc
     2821 gttttatttt tgctgctgtc aggataccga cgaagacagg agtaccgtgc tgtacttgca
     2881 ggactgcgcc gagcaggcag ggcaggagtc tcggtttatc tacattgaag agctcggttt
     2941 gggcgtcggc ggcgtactga ccgatcttga tgataatgtc atccagcgtg catttaagct
     3001 gtatccgctg gagtggatga tgcgtgacga taacggcccg ctgctgcgca agcgtcgtga
     3061 gcaatgggtg gagccgttat ggaaaagtat cttgagtaat aaagggctaa tgccgctgct
     3121 ttggcgcttc ttccctggtc atcctaacct tcttgcatcc tggttcgatg gtgaaaaacc
     3181 gcagattgcc gctggcgaaa gctatgtgcg taaaccgatt tactcgcgcg aaggcggaaa
     3241 cgtcaccatt ttcgacggtc agaataacgt tgttgaccac gctgatggtg attacgccga
     3301 tgaaccgatg atctaccagg cgtttcaacc tctgccgcga tttggcgata gctacacact
     3361 catcggtagc tggattgtcg atgatgaagc gtgcggaatg gggatccgtg aagataacac
     3421 gctgatcacc aaagacacct cacgtttcgt tccgcattac attgctggat aagaatgttt
     3481 tagcaatctc tttctgtcat gaatccatgg cagtgaccat actaatggtg actgccattg
     3541 atggagggag acacagtgca ctggcaaact cacaccgttt ttaatcaacc tataccatta
     3601 aataacagca acttatacct gtctgatggc gcgctttgcg aagcggtaac gcgtgaaggt
     3661 gctagctggg atagcgattt tctcgccagt attggtcagc agttaggaac ggctgaatcc
     3721 cttgaactgg ggcggctggc gaatgtgaat ccgcctgaat tattgcgcta cgatgcgcaa
     3781 ggacgccgtc tggacgatgt gcgttttcac cccgcctggc acctgctgat gcaggcgcta
     3841 tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg ctcgctccgg cgcatttgtg
     3901 gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg ctgggtcgtt atgtccgata
     3961 acgatgacct ttgccgccac gccattgttg ttacagatgt tacccgcgcc gtttcaggac
     4021 tggaccacgc cgctgctgag cgatcgctac gattctcact tattgccagg tgggcaaaaa
     4081 cgtggcttgt tgatcggcat gggaatgacg gaaaagcagg gcggttccga tgtcatgagc
     4141 aacaccaccc gcgcagagcg tctggacgat ggttcttatc ggctggtggg gcataaatgg
     4201 tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg cacagaccgc gggcggtttg
     4261 tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac gcaacgcgat tcgcctcgag
     4321 cggctgaaag ataagctggg taatcgctct aacgccagct gcgaagtgga gtttcaggat
     4381 gccattggct ggttgttggg gcaggaaggg gaaggaattc gtctgatcct gaaaatgggt
     4441 gggatgacgc gttttgattg cgccctgggt agccatgcca tgatgcgccg tgcattttcg
     4501 ctggcgattt accatgcaca tcaacgccat gtttttggta atccattgat ccaacagcct
     4561 cttatgcgtc atgtcttaag tcgcatggca cttcagcttg aagggcaaac ggcgttgctg
     4621 tttcgtcttg cgcgagcgtg ggaccggcgc gccgatgcca aagaagctct gtgggcgcgt
     4681 ttatttacgc ctgcggcgaa atttgtgatc tgcaaacgtg gcattccgtt tgtggccgaa
     4741 gcgatggagg tgctgggcgg cattggttat tgcgaggaga gcgagctgcc gcggctttac
     4801 cgggagatgc cggtaaacag tatttgggaa ggttccggca atattatgtg cctggatgtg
     4861 ctgcgcgttc tcaataagca agcgggcgta tacgacttat tgtcggaagc atttgtggaa
     4921 gtgaaagggc aggatcgcta ttttgatcgc gcggttcgtc gtttacagca gcagctgcgt
     4981 aagccagctg aagaattggg gcgagagatt actcatcagc tattcctgct gggctgcggt
     5041 gcgcaaatgt tgaaatatgc ctctccgcca atggcgcagg cgtggtgtaa ggtgatgtta
     5101 gatacgcgcg gcggcgtacg gttgtcagag cagatccaga atgatttatt gctgcgggcg
     5161 acgggaggag tgtgtttgta agcgtatacg actgatgcga cgcaggtttc gattaactaa
     5221 atgaaatatt ttaaaattgt aggccggaca aggcgctcgc gccgcatccg gcattgttca
     5281 tttgctgtag atgacactca cgcatacagt atcgcctgta cgcgccagtt atcaggctgc
     5341 tggtcttcgt acatggtaat aatgcggtac catgacgcac cttgttcatc ggcctttaac
     5401 gccacgatac gttccacgtc ctgcggatcc cctgaaatat tgctaactga gatctgacct
     5461 atttcattca ggcccgtcac gcagtcagca ctggcgaatt ctgcagactg tgccgtggcg
     5521 ctcgcaggat aagtcactaa ttgcagcgag ggtgaggcaa gtaattgttt catcgtcagc
     5581 tccatttttc gtttccccac atgttgcagg gcattccatc gcggtaataa acgcccgagc
     5641 ttccagccgg taatttattg tgcacaggta aacgcatagg gacgcaaagc aatgtaaagc
     5701 tgactatgta agttgtctga tttatttacg gtacaaaatg gcttgtgaat accactgtcc
     5761 tgtcacgatg gtttcgtcca ccatgacgac aacgtaataa tcagcttttg cagcgacagc
     5821 tttcgctctg atttctgcta atgcgtcatc cggagaaccc cgaaccatcg tgcttacgct
     5881 acctattcgc tgtaaccctt gcgtctggtc gcggcgaatc tcttgcggat ggtccgttac
     5941 tggcggtgct ggctgtggcg taccttgcag tgcgctacag gcacttaaca tcagcaccaa
     6001 taataaactg gcaaaccggt aaataacgct attacgtttc ctgctaacca tagtgtagtg
     6061 ccttattaat ttaactttgg gggaatgttc ccgaattgtt acctggtacg cgattttcga
     6121 atgaaaacgt gacgaaagcg taacccacat ctcaacatta tgaactaata ccgccagcag
     6181 aaacttgtgc taaggatttc ttcatgtcat accagacaaa aaggagagac agatgattga
     6241 aatagaatca cgcgagctgg cagatattcc cgttcttcat gcttatcctg tcgggcaaaa
     6301 agataccccg ttaccgtgtg tgatttttta tcacggcttt acttcgtcca gtctggtgta
     6361 tagctatttt gccgttgcgc tggcgcaggc tggtttgcgg gtgatcatgc cggatgctcc
     6421 cg
//
LOCUS       DAWDRC010000173         6420 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5597_length_6420_cov_2.412726, whole genome shotgun sequence.
ACCESSION   DAWDRC010000173 DAWDRC010000000
VERSION     DAWDRC010000173.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5597_length_6420_cov_2.412726"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5298..5307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1395 a         1620 c         1738 g         1657 t
ORIGIN      
        1 tttcggcgca gttctgctgg cagcggcgct ggtcggtacc ggttcatcgg tctttcatcc
       61 ggaatcttct cgcgtggccc gtatggcttc cggcgggcgt catggcctgg cgcaatctat
      121 atttcaggtc ggcggaaact ttggcagttc cctgggcccc ttgctggcgg cggtgattat
      181 cgcgccttat ggcaaaggca acgttgcctg gtttgtgctt gcggcactgc tggcgatcgt
      241 ggtgttggcg caaatcagcc gttggtactc ggcacagcac cgaatgaata aaggaaaacc
      301 caaagcgacg attatcaatc cactgccgcg caataaagtt gttctggcgg tcagcattct
      361 gttaatcctc attttctcga aatatttcta tatggcgagc atcagcagct attacacctt
      421 ttatctgatg caaaaattcg gattatctat ccagaatgct cagcttcatt tgtttgcctt
      481 cctgtttgcc gttgcggcag gtacggtgat tggcgggcct gtaggggata aaattggacg
      541 gaaatatgtg atttggggct ctatcctcgg cgttgcgccg tttacgctga ttttacccta
      601 cgccagcctg cactggacgg gggttttaac ggtgattatt ggatttatcc tcgcttcggc
      661 attctctgcc attctggtct atgcccagga gctgcttccg ggacgtatcg gtatggtttc
      721 tggactattt ttcggttttg ctttcggcat gggaggtctg ggggcggcag ttctggggct
      781 tatcgccgac cacaccagca tagagttagt ctataaaatc tgtgctttcc tgccactatt
      841 ggggatgttg accatattcc tgcctgataa ccggcataaa gactgatttc attgcagcca
      901 aaggcaaact ttggctgcat cgttttacag tcgccataag cctttcctct gttaaaccgc
      961 cttctgtttt ccgatccgtg ctttttttgc agctaattcc atttttctgc aaaattcaac
     1021 aattattcat aaacatcatc aatgaaattg tcataaacta ttacctgtaa ttttggtttt
     1081 cccggccaaa aatggacaat aaccaccagg aaaaggagac ggaatgcatc acgccacccc
     1141 gcttatcacc accattgttg gcggccttgt gctcgccttt atcctcggca tgctggccaa
     1201 taaactacgt atttctcctc tggtgggata tctgttagcg ggtgtgctgg caggaccatt
     1261 cactccgggc tttgttgccg ataccaagct tgcgccggaa ctggctgaac tgggcgttat
     1321 tttgctgatg ttcggcgtcg gtttgcattt ttcgctcaag gatttgatgg cggtaaagtc
     1381 catcgccatt cccggcgcga tcgcccagat agccgtggcg acgctgctgg gtatggcgct
     1441 ctccgccgtg ctgggctggt cgttaatgac cggtatcgtg ttcggcttat gtctttccac
     1501 cgccagtacc gtggtgttac tgcgcgcact tgaagaacgg caattaattg acagtcagcg
     1561 tgggcaaatc gccatcggtt ggttgattgt ggaagacctg gtgatggtcc tgacactggt
     1621 attgctaccc gcggtagcag gaatgatgga acagggcgat gtgggctttg ccactcttgc
     1681 agtcgatatg gggatcacca tcggcaaggt gatcgcattt atcgccatta tgatgctggt
     1741 aggtcgccgt ctggtgccgt ggattatggc acgcagcgca gcaaccggtt cccgcgagct
     1801 gtttaccctg tcggtgctgg cgctggcgtt agggattgcc tttggtgcgg tagagctgtt
     1861 tgatgtctcc tttgcactcg gtgcgttctt tgccgggatg gtactgaacg agtctgaact
     1921 gagtcaccgt gccgcccacg atacactgcc attgcgcgac gcgttcgcgg tgctgttttt
     1981 tgtctccgtc gggatgttgt ttgatccgtt aattctgatt cagcaaccgc tggcagtact
     2041 ggcgacgctg gcaattattc tgtttggtaa atcggtcgcg gccctgttcc ttgtgcgttt
     2101 gtttggtcac tcccagcgca cggcgttaac catcgccgcc agcctggcgc agattggtga
     2161 gttcgcgttt atcctggcgg gactaggaat ggcattaaat ttactgccac aggccggaca
     2221 aaacctggta ctggcagggg cgatcctgtc gattatgctc aacccggtac tgttcgcact
     2281 actggagaaa tatctggcga agaccgaaac gctggaagag cagacgctgg aagaggcaat
     2341 cgaagaagag aagcagatcc cagtggatat ttgcaaccat gcgctactgg tgggttacgg
     2401 tcgtgtaggc agcctgctgg gggagaaatt gctcgcctct gatattccgc tggtggtgat
     2461 tgagacgtca cgaacccgtg ttgatgagct gcgcgagcgc ggggtccgcg cagtattggg
     2521 caatgcggcg aacgaagaaa ttatgcaact ggcgcatctg gaatgtgcaa aatggctgat
     2581 cctgacgatt cccaacggct atgaagcggg cgagattgtg gcatctgccc gcgcgaaaaa
     2641 tccggatatt gagattattg cccgcgccca ttatgacgat gaagtggcgt atatcaccga
     2701 acgtggtgcg aatcaggtgg tgatgggtga gcgtgaaatt gcccgtacta tgctggaact
     2761 gctggaaacg ccaccggcgg gtgaggtggt gacggggtaa acggtggttg ccggataata
     2821 ggcgcggata tgtaggcctg ataagacgcg ccagcgtcgc atcaggcatt gaaagcaaaa
     2881 tgccagatgc ggcgtgaacg ccttatctgg cctacgaacc gacgataact taacgatccc
     2941 agtaagactc ttccaggctg tcttcacgct ccggcaagcc gcgcgttaaa cgaggtgaat
     3001 gctggttcag tacctgatag ctcacacggt tagcatattt acacacctgc gctaacgatg
     3061 aataagttaa ccaggtgaat ttatgtttgc tggagtttgg tacgttgcta cggtggtagc
     3121 tgttggcggt aatgtcatgc agcaacgctg ccaatgcgcc atcccctgct ccgttagtgt
     3181 tcatgatttt ttccggcccg cccatatacg gcgcaatgtg cgaatagaca cgcagcggat
     3241 tctggcaatc cttgtggcgc atggcgcggc taaactcata ctggttaaat tccgctatag
     3301 cacccggcag cagcggatgc tgggttttac gtttcgcttc gtcttcggta aagcctgcca
     3361 tatacaagcc gattggcccg gcggtgcaca gcaccagatc tacccagtcc agcgccttgt
     3421 cagatgccaa caacggatcg ctttctccgg tcaacgcttc ggcttcatct tcgttcatcg
     3481 caaggataga gacgtggtct ttgaggaatt gctgccacca ctgcggattc tcggcaatga
     3541 caaacttggt acccagtgtc agcaccaccg gtacgttata tttcttcgcg tactcaatgg
     3601 ctttcatggt tgcttccggc atgggttcac ccggcttaca acgcaccaga taagaggtga
     3661 ggaccagtgc cgaggctccg gcaatcacat cttccggaat actttcagcc cgcagctggt
     3721 tcatgtggcc ggggctgata gcaaaggtac gttccccgga ctcgccaatc agcgtaaagc
     3781 aacgaccaat tgggccatcc acgccttgta gatagttaag atcggtacgg ctggaggtgt
     3841 tacacaggta acgataggca tagctgccaa tttcaatatt gctgcacatg acgcccagca
     3901 gcaccgaacg gtcgtccgcg agcaccgagt agttgtgcat ggtgttacca atggtgccac
     3961 ccgcaaactg atgggtaatc aggtttttct gttttagttc ctgataaagc gcttcggcaa
     4021 cgtcatcctc aatcaccaat gaatgcccgg cgcttaatcc ataacgctca atgaattcat
     4081 catccacttt cgcttcaata tcgaccagcg tttgatcgat acccactacc caggcggcgc
     4141 tggtttcgtt ttctggctgg aattgctgaa gcagcggatc gcgtgcgttt acggggaagt
     4201 aatgtttgga tttacgttta ccgggaaatt tcatggttgt ttttacgggt ggttaactga
     4261 gcggcgaatg ttaacacaat ctgacgggag cgcgcgagac gcagccagtt tcaatatcag
     4321 caggcgggat cgcattttca tggcagtggt tccttacaat gacgtaattt gaaaggagtt
     4381 tttgcatgaa gccggaaaac aaattacccg ttctggacct tatctctgct gaaatgaaga
     4441 ccgttgtgaa tactcttcag ccggatttac cgccctggcc tgcaacggga gcgattgctg
     4501 agcaacgaca gtattacacg cttgagcgcc gattctggaa tgtgggcgct ccagaaatgg
     4561 caaccagagc ttacagggtt ccaacaaaat atgggcaggt gaaaacacgt atcttttatc
     4621 cgcagccaga tagcccggcg acgctatttt atttgcatgg aggcggtttt attctcggca
     4681 atctcgatac ccacgatcgc atcatgcgcc tgctggcaag ctacagccaa tgtacggtga
     4741 ttggcattga ttacaccctt tcacctgaag cgcgttttcc gcaagcgata gaggaaattg
     4801 tggctgcctg ttgttatttc caccagcagg cggaggatta tcaaatcaat atgtcccgca
     4861 ttggttttgc tggtgattcc gcaggtgcca tgctggcgct cgccagtgcg ttgtggttgc
     4921 gcgataaaca gatcgattgc ggtaaagttg cgggcgtttt gctgtggtat gggctttacg
     4981 gattacggga ttccgtgact cgtcgtctgt tgggtggtgt ctgggatggc ttaacgcaac
     5041 aggatttgca gatgtatgaa gaggcgtatt taagcaacga cgcggaccgc gagtcgccat
     5101 attactgcct gtttaataat gatctcactc gcgaagttcc gccctgtttt attgccgggg
     5161 cggagttcga tccactgctg gatgacagcc gtctgcttta ccagacgtta gcggcacacc
     5221 agcaaccttg tgagttcaaa ctctactccg gtatgctgca cgcctttttg cattattcac
     5281 ggatgatgaa aaccgccnnn nnnnnnnaaa accgccgacg atgctctacg cgacggtgct
     5341 cgattcttta gcgataggct gcaacaagat tagccatcat ttcgatatgc tccggtgtag
     5401 cattaagcgc cggaatatat tcgtattttt tcccgccagc gccgaggaag acttcacggt
     5461 tttgctcggc aatctcttcc agcgtctcca gacaatccgc agcaaagccc gggcacatca
     5521 cctgaatatg acccacgcct ttttctccga gcattttcag cgtttcgtcg gtataaggca
     5581 tcagccaggg ttcccggcca aagcgcgact gaaaggtcat catcactttt tccggtgcca
     5641 tctccagtgc ggaagcgagt tcgcgagttg ttgtgcggca acgttgcggg taatcatcgc
     5701 cttcatctgc ataacgctgg ggaatgccat gataagagag caacagcaga tccggttcgc
     5761 catgtttggc aaaagaagca cgtacgctgt tcgccagtgc attaatataa tcgtggttat
     5821 cggcgtaatc acgaataaac gatatccccg gaatgctacg tttgcgcgcc agaattcgcg
     5881 ccagttcatc ccataccgca ccgaccgttg aacaggagta ttgcggataa agcggcagca
     5941 ccacaatatg atctacatgc tctgccagaa gttcatctac ggcgctttcc agtgatggcg
     6001 agccgtagct cattcccagc gccacgggcg tctccggtaa acgttgcgcc agcgcctgct
     6061 gttgctggcg gctgtaaacc atcagcgacg agccaccttc catccagaca gaggcataca
     6121 gcttcgccac acgcggcgaa cgcagcggca aaatcacgcc gcgcagcaac ggccaccata
     6181 acaaccgtga ggtatcaacc acgcgtctgt cgcttaaaaa ttgtttcaga tagcgtttta
     6241 ccgcttcagg tgtgggggca tcaggcgtac ccaggtttgc cagcaggata ccggttttag
     6301 tttgacgcat taccgcctct tatcgattca acttgttgat aattgtagcg gaaaagtgaa
     6361 gaagaagaac taattgctgt aagaggtatt gcctgaggag gatgagcaga cctgcccgag
//
LOCUS       DAWDRC010000174         6406 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5614_length_6406_cov_2.321997, whole genome shotgun sequence.
ACCESSION   DAWDRC010000174 DAWDRC010000000
VERSION     DAWDRC010000174.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5614_length_6406_cov_2.321997"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    644..653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5660..5669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1468 a         1770 c         1591 g         1557 t
ORIGIN      
        1 ggcggcaaat actggttaga taaaccgtta ctttttagcc atttactgtc gtgaactttc
       61 agcgcctcgt tcgccgcaaa tcgcaccagg cccgcgcaat cctgttgata ccagcgtggg
      121 cttggcccct ggcggagctg ttcctgtgca atgcgcacaa accacgcgcg aaacagcccg
      181 gattgttcga catttagcat ttcactatgg gcaacaacac aatacagcca gcaaatcagc
      241 accagcagcc cgtgtctcat agcggttgcc aggttatggg tagccactgc caggcggcac
      301 cgggttccat ctgggccagc ttcatgacat aacgcggttg ttgagataaa gcgtccagct
      361 tcggcattaa taaagtttgt gcggcgttat aaaaaaccgg ttcgagattc ttcggcagac
      421 tggtcagcgt ttcgttacgc agcagtttcg ccattccttg tggattgata tagagcggaa
      481 cgatgccatc agtgggtatt acatccacca ttgccgggcg ggttttattc agtgcttgca
      541 gggcgttatt aaccagcgtg tcatcgaggg agaaaagcag cgttttgttt tgcatcgcca
      601 gcgacacgcg gaaaaaataa tccgacatta attgatcggg ttgnnnnnnn nnncgctttc
      661 ggatactgac cgtatcggga actcacttcg cgacgccaga tttgcgcttc gccctgctga
      721 gtctggctta ccggcaaaac accttctggc gctttgcttt cgtgcgcacc aatattttgc
      781 gtaaacagtt tccctggcaa ttgcgcctgt tcggcagtgc catcaaactg accaacaaac
      841 agtggggttt gcaattttga gtcttcatac cagcacagcc ccgcgccacc gtctaacgcc
      901 ccattcagct tgtcgttttc ctggctgatg tgcgaaagca tctcttcggc aataccgtgt
      961 gaatacggca ccgccacaca gaagctggct ccggcaggca tactgttcca taccggcgta
     1021 aaatcgaaac tggcatctac gctggcggat tcatcgttta acgccagaaa actgtgccag
     1081 ccgtcgttac ccatttcgaa gcgcacgcca gcaaaagaag gcactaatcg ctggtagcca
     1141 aaccccagca acctggcgct gactacgatg cgctggcgta ctggcgtttt ttcggcagta
     1201 cgcgcttcca ggccaaagct ggcttgccag cgttttttac cactcaacaa atcacttgcg
     1261 atcgccgtgg cttcggtatc ctgttggtca tctttaaaca gcatatccga actggaaaac
     1321 gccaatattt tgtcctgata agtcgcaaac atcaggacgt tattgccgtt atagcgcaac
     1381 tgataaacag gaatggtttc attatttagc gtgatgctac taatttccgt cttgcttaac
     1441 tggctgtcgc tagtagcagc aaacagcaat ggctccagca gcttgcttaa accactgcgc
     1501 tggataagca ccatataatg tgaaagatga ccttgtttat catgccacag tgccgcctgc
     1561 gcgggctggt ctaagagtga cgaaaagagc ttatctttca gcgtgagatc gtgttcatag
     1621 acaatgcggc gaatgctgcc ttcaatgccc agacgatcgg catgattctg ataatagaaa
     1681 acgaaatctt cgctcagaac atcgtggaga aacggaatgg taaggagatc tttgggaagc
     1741 tggctcagag agtcgctgtc gagaaagagg tccggctcat tgagatcgat ttgcagattg
     1801 ttgtgcacca ccagtggcga caacgttttt tctggcccac tgccagagta ttgtaatgcc
     1861 catacgccag cggatagcag cgctattgcc ccaaaaccaa caaggccata aaaccgccag
     1921 cctttcgcct ttttttcacc actcattgcc acattccttg tgtatagcca gccatttttt
     1981 acgggcacag ccaaacttta ccgcgcccta atacgacaaa agcccagact ttacagcctg
     2041 gacttttcaa ttcaaaaaag ggagatggct cccttttggc atgaagcgat gaaatttttc
     2101 ttcgtctggc tcgtcgtcaa cgtccacttc cggagcgatt tcatcgtccc cttccgcggc
     2161 actgccgtcg atggtatcca gatcttcctc gtcaaccggt tcagcaacac gttgcagacc
     2221 caccacgttt tcatcttccg aagtacggat aaggattacg ccctgggtgt tacggcctac
     2281 gatgctgatt tccgaaacgc gagtacgtac cagcgtaccg gcatcggtga tcatcatgat
     2341 ctggtcgcag tcatctacct gcaccgcgcc aaccactaaa ccgttacgtt cggtaacctt
     2401 gatggagata acccctttcg tcgcacgcga cttggttggg tattccgcca ctgcggtacg
     2461 tttaccataa ccgttctgcg ttgcggtgag gattgcgcca tcgccacgag gcacgatcag
     2521 agagacgact ttatcgcctt cgcctaaacg aataccacga acaccggtgg tgttgcagcc
     2581 catcgcacgg acagaagact ctttaaagcg caccacttta ccttcagcgg agaacagcat
     2641 tacttcgtct tcgccgctgg tcaggtcaac gccgatcagc tcatcacctt caaccagttt
     2701 gatcgccact ttaccggcgg tacgcagacg gttgaactcg gtgaggacgg ttttcttcac
     2761 agtaccgtta gcggtcgcca tgaagacttt cacgccttct tcaaactcgg tcaccggcag
     2821 gatcgcggtg atacgttcgt cctgttccag cggcagcaga ttgacgatcg gacgaccacg
     2881 tgcgccacga gtagcttctg gtaactggta gaccttcatc gaatagacgc ggccacggct
     2941 ggagaagcac agaatatggt cgtgagtgtt cgccaccagc agtcggtcga taaagtcttc
     3001 ttctttaata cgtgcggcag atttaccttt cccgccacga cgctgcgctt cgtattcaga
     3061 aagcggctga tacttcacgt aaccctggtg agaaagcgtg accaccacat cttcctgggt
     3121 gatcagatct tccaggttga tgtcggcgct gttagcggtg atttcggtac gacgtttgtc
     3181 accgaactgt tcacgaacca actccagctc ttcacggatc acttccatca gacgatcggc
     3241 gctaccaaga atacgcaaca gttccgcaat ctgatccagc agctctttgt attcgtcgag
     3301 cagtttttcg tgctcaaggc cggtcagttt ctgcagacgc agatccagaa tcgcctgagc
     3361 ttgctgttcg gtcaggtagt acagaccatc acgcacgccg aactctggct ccagccattc
     3421 cggacgcgca gcatcgtcgc ccgcacgttc cagcatcgca gcaacgttgc ccagctgcca
     3481 cggatttgca accagcgcgg ttttcgcttc tgcaggcgtc ggcgcatgac ggatcagttc
     3541 gatgatcggg tcgatgttcg ccagcgccac ggctaatgct tcaaggatat gagcacgatc
     3601 gcgagcttta cgcagttcga aaatagtacg acgggtcact acttcacggc ggtgacgaac
     3661 aaacgccgcg atgatgtctt tcaggttcat gatcttcggc tgaccatggt gcaatgccac
     3721 catgttgata ccgaaagaaa cctgcaactg ggtctgggag tagaggttgt tgagcacaac
     3781 ttcaccgacc gcatcgcgtt tcacttcaat cacgatgcgc ataccgtctt tgtcagactc
     3841 gtcacgcagc gcgctgatgc cttccacgcg tttttctttt accagttccg caatcttctc
     3901 gatcaggcgc gctttgttca cctgataagg aatttcgtgg acgataatgg tttcacgtcc
     3961 ggttttggcg tcaacttcta cttcggcacg ggcgcggata tataccttgc cgcgaccggt
     4021 acggtaagct tcttcaatac cgcgacgacc gttaatgatt gccgccgtcg ggaagtccgg
     4081 ccccgggatg tgttccatca gcccttcaat gctgatgtct tcatcatcga tatacgccag
     4141 acaaccgttg atgacttccg tcaggttgtg cggcgggatg ttggttgcca tacctacggc
     4201 gataccggaa gaaccgttca ccagcaggtt aggaattttg gttggcatga cgtccggaat
     4261 tttttccgta ccgtcatagt tatcaacgaa atcgaccgtc tctttttcga gatcagccat
     4321 cagttcatgg gcaattttcg ccagacggat ttccgtataa cgcattgccg ccgcagagtc
     4381 gccgtcgatg gaaccgaagt taccctgacc gtctaccagc atgtaacgca gcgagaatgg
     4441 ctgcgccata cggacgatcg tgtcataaac cgccgagtca ccatggggat ggtatttacc
     4501 gattacgtca ccaacgacac gggcagattt tttataggct ttgttccagt cattgcctag
     4561 tacgttcatg gcgtaaagta cgcgacggtg taccggcttc aggccatctc ggacatctgg
     4621 cagcgcacgg ccaacaatga ccgacatcgc ataatccaga taggagctct tcagctcttc
     4681 ctcaatgttg accggtgtaa tttctctcgc aaggtcgctc atctaaccgc tatccctcta
     4741 ctgtatcccg gattcaaagg tcgcaaatta taacacagcc gcgcagtttg aggtaaacct
     4801 atacgcttta ttcacatcca atgcctgata tactcgtttg tcttgccaat tacggagtag
     4861 aagtgccaat gaatgccgaa aaatcgccgg taaaccacaa cgtagaccac gaagagatcg
     4921 ctaaatttga agccgtcgcc tcccgctggt gggatcttga aggtgagttc aaaccgctgc
     4981 accgcattaa cccgctgcgt ctgggctata ttgccgagcg tgctggcggt ttatttggca
     5041 aaaaggtgct cgatgtcggt tgtggcggcg gcattctggc cgagagtatg gcgcgggagg
     5101 gcgcgacggt gaccggtctg gatatgggct ttgagccatt gcaggtggca aaactgcacg
     5161 cactggaaag cggcattcag gtggattacg tgcaggaaac cgtggaagag cacgcggcaa
     5221 aacatgccgg acagtatgac gtggtgacct gtatggagat gctggagcac gtccccgatc
     5281 ctcagtcagt ggtcagagcc tgtgcgcaac tggtgaaacc gggcggcgat gtctttttct
     5341 cgacgcttaa ccgcaacggc aagtcatggc tgatggcggt ggttggtgct gaatatattt
     5401 tgcgcatggt gcctaaaggt acacatgatg tgaagaagtt tattaaaccg gcagaattgc
     5461 tgggctgggt ggatcagacc agtcttaaag aacggcatat gaccgggctg cattacaacc
     5521 ctataactaa ttcctttaag ctcggccccg gcgtggatgt gaactatatg ctgcatacgc
     5581 agaataagtg aggttgttgt ttggattcat ccggcaaccg cgccaagcct gatgcggcgc
     5641 ttgccgcgtc ttatcaggcn nnnnnnnnnt gctccccgta ggccggataa ggcgtttacg
     5701 ccgcatccgg caacagtgcc ggtcagacag cgattaccat ttcactgtca tcgacaaaaa
     5761 ccctgccgtc tgggcaaaat catcactccc tttctgccac gccacgctac cacgcagcga
     5821 aactcgctga ctgatattgc ccgttattcc cacttttatt tcaccccgtt gcttcgccgc
     5881 atcgtcactg atagtgctgc catcttcttc aattcccgtc gaatggggat cgtgataata
     5941 attcagatct aacgttggcg tgacaccaac agcagtacgc cattcgctgt gtaaacccag
     6001 ccgcgtctga atatcatcac cctgcgattg tgacacgcgc gcatggttag cggcggtaaa
     6061 atcatcctgc tgcacgccct gataaatcac ctgcgtctgc ggttcaatca ccacaccatg
     6121 ccccggtaac cactgataac cggcttccag cgaggcgata atccccgacg agtggtaatg
     6181 atccacgcca tcgtcttgtt cggaaacatc attgttaaac cacgcatatt gcagccagct
     6241 atccaaccag gccccctgct tctgattacc gtgctgatac cagcttgagg tcagcccaac
     6301 ggcataaccg tggttctggt tatcggcgcg agttccggtc atattcgagc ggctgtcgcc
     6361 ctggttatcg ctgtagccac caacaatccc aagcatccac tcgcca
//
LOCUS       DAWDRC010000175         6400 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5620_length_6400_cov_2.653743, whole genome shotgun sequence.
ACCESSION   DAWDRC010000175 DAWDRC010000000
VERSION     DAWDRC010000175.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5620_length_6400_cov_2.653743"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4036..4045
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1525 a         1592 c         1735 g         1538 t
ORIGIN      
        1 ttgtgattac ccctcctttg atgccaggtc cggcgttgct ggttgacgat atcgtggatt
       61 ctgcatggac gctgacagtt ctgacagcac tgttacgcca ggcaggttgc ccgacggttt
      121 atcctcttgc ccttgcttct acctcggtaa aaaattgatg aatctttcag ccaatgcaca
      181 agcaactctc ctgctaacca gcgatttttc tcgcgcggcg gcgagtgagt ataaacctct
      241 tagtaatagt gaatggggga agtttgcatt atggctgaag caccaacgta tcagtcccgc
      301 cgagcttctg gtgccgcaac cgcaagagaa acttacaggc tggagcgatc cgcgtatttc
      361 tcaggagcgt attcttggct tgctggcgcg tggtcatagt ctggcgttgg cggtagataa
      421 gtggcaacgc gccggtttat ggatcttaac ccgcggagat gccgattatc ccgttcgctt
      481 gaaaaaccga ttgcgaacgg atgcacctcc cgttttattt ggctgcggga ataaagcatt
      541 actgcaagcg gaaggtatgg cgattgttgg ctcgcgagat gctccgactg acgatttgcg
      601 ctatacccaa caactggccg cgaaactggc ccaacagggg atttgcgtta tctctggtgg
      661 tgcgcgaggt attgatgaat gtgcaatggc ctcggctctg gaggtcgggg gaactgccgt
      721 tggcgtatta gctgatagct tgttaaaaac gagtacgtta gtgaaatggc gtgaagggct
      781 tatagcaggc aacctggtgt tgatttcgcc gttttaccca gaggtacgtt tcaccgtcgg
      841 caatgcgatg gcgcggaata aatatattta ttgccttgct gaaagcgcaa tggttgtacg
      901 tgcgggaatg accggtggaa cgataaccgg ggcgatggag gcattaaaac atcagtggct
      961 gcccatgcag gttaaaccaa atcaggatat gcaatcagcc aattcacgat tagtagaaaa
     1021 tggggcgtca tggagtgctg aacaggctga gaatgtgacg atcagactgc cagacgttcc
     1081 tgggctgatg tatgacagag cactccgtaa cgctcaacca gaactgtttt cgctgcacga
     1141 agatgacgca aattacgcag taatgcccgc gcatacccct gtcgattttt atcaactctt
     1201 tgtggcggaa ctggcgatcc ttgcaaagga atcgataagt attgaaaggc tggcgtcttg
     1261 tactggttta accatcgaac aaattagtgt gtggctgaac cgcgcagaag aagagggaag
     1321 ggttatccga ttgggtgaag gtcattatca gttcaggtaa taaaacaggt gccgcgtctg
     1381 gttgtgtgca acacctgttt gaaccttacc ccttcatcca cattcgaatg ccgtcgagga
     1441 acatctgagt tgccatcatc accagaatca atcccatcag gcgttcaagt gcgttcaccc
     1501 ctttctcgcc cagcagacgt aaaaatagcg aagactgtag caggatgaca aaggtgccgc
     1561 cccaggccag cagcagagca atcaccagat gtcccatctg attcgggtac tgatgagaca
     1621 acaacatcag cgtggcgaga atagtcggcc cggcgaccag cggaattgcc aacggcacga
     1681 taaatggctc ttcacctgcc ggaagcccgc tgctatttcc tgaagcgctg gggaaaatca
     1741 tcttaatggc gatcagaaac agaatgatgc cgccagaaat ggagacggtt tctgcccgca
     1801 ggctaagaaa tgccagaatt ttctcgcccg caaacaggaa caccagcatc acaaggagag
     1861 caataagcaa ctctcgcacc atgattgccc gccgtctttt cggttcagta tgtttcagta
     1921 cggacatgaa aataggtagg tttccgagcg gatccataat caggatcaat aaaactgttg
     1981 cagaaatgat ttcattcata actcaaattc cctgataatt gccgcggact ttctgcgtgc
     2041 taacaaagca ggataagtcg cattactgat ggcttcgcta tcattgatta atttcacttg
     2101 cgactttggc tgctttttgt atggtgaaag atgtgccagg aggagaccgg cacatttata
     2161 cagcacacat ctttgcagga aaaaacgctt atgaaaaatg ttggttttat cggctggcgc
     2221 ggtatggtcg gctccgttct catgcaacgc atggttgaag agcgcgactt cgacgccatt
     2281 cgccctgtct tcttttctac ttctcagctt ggtcaggctg cgccgtcttt tggcggaacc
     2341 actggcacac ttcaggatgc ctttgatctg gaggcgctaa aggccctcga tatcattgtg
     2401 acctgtcagg gcggcgatta taccaacgaa atctatccaa agcttcgtga aagcggatgg
     2461 caaggttact ggattgacgc agcatcatct ctgcgcatga aagatgacgc catcatcatt
     2521 cttgaccccg tcaatcagga cgtcattacc gacggattaa ataatggcat caggactttt
     2581 gttggcggta actgtaccgt aagcctgatg ttgatgtcgc tgggtggttt attcgccaat
     2641 gatcttgttg attgggtgtc cgttgcaacc taccaggccg cttccggcgg tggtgcgcga
     2701 catatgcgtg agttattaac ccaaatgggc catctgtatg gccatgtggc agatgaactc
     2761 gcgaacccgt cttctgctat tctcgatatc gaacgcaaag tcacaacctt aacccgtagc
     2821 ggtgagctgc cggtggataa ctttggtgtg ccgctggcgg gtagcctgat tccgtggatc
     2881 gacaaacagc ttgataacgg tcagagccgc gaagagtgga aagggcaggc ggaaaccaac
     2941 aagatcctca acacatcttc cgtaattccg gtagatggtt tatgtgtgcg tgtcggggca
     3001 ttgcgctgcc acagccaggc attcactatt aaattgaaaa aagatgtgtc tattccgacc
     3061 gtggaagaac tgctggctgc gcacaatccg tgggcgaaag ttgttccgaa cgatcgggaa
     3121 atcactatgc gtgagctaac cccagctgcc gttaccggca cgctgaccac gccggtaggc
     3181 cgtctgcgta agctgaatat gggaccagag ttcctgtcag cctttaccgt gggcgaccag
     3241 ctgctgtggg gggccgcgga gccgctgcgt cggatgcttc gtcaactggc gtaatcttta
     3301 ttcattaaat ctggggcgcg atgccgcccc tgctagtgcg taatacagga gtaagcgcag
     3361 atgtttcatg atttaccggg agttaaatag agcattggct attctttaag ggtggcggaa
     3421 tacacgagta ttcacagcct tacctgaagt gaggacgacg cagagaggat gcacagagtg
     3481 ctgcgccgtt caggtcaaaa aaatgtcaca accagaagtc aaaaattcag ttggatgggg
     3541 tgacacaata aaacaggaag acaagcatgt ccgatcgtat cgatagagac gtgattaacg
     3601 cgctaattgc aggccatttt gcggatcctt tttccgtact ggggatgcat aaaaccaccg
     3661 cgggactgga agtccgtgcc cttttacccg acgctaccga tgtgtgggtg attgaaccga
     3721 aaaccgggcg caaactcgca aaactggagt gtctcgactc acggggattc tttagcggtg
     3781 tcattccgcg acgtaagaat tttttccgct atcagttggc tgttgtctgg catggtcagc
     3841 aaaacctgat tgatgatcct taccgttttg gcccgctaat ccaggaaatg gatgcctggc
     3901 tattatctga aggtactcac ctacgtccgt atgaaacctt aggcgcgcat gcagatacta
     3961 tggatggcgt cacaggtacg cgtttttctg tctgggctcc aaacgcccgt cgggtctcgg
     4021 tggttgggca attcannnnn nnnnnaattc aactactggg acggtcgccg tcatccgatg
     4081 cgactgcgta aagagagcgg aatctgggaa ctgtttatcc ccggggcgca taacggtcag
     4141 ctctataaat acgagatgat tgatgccaat ggcaacttgc gtctgaagtc cgacccttat
     4201 gccttcgaag cgcaaatgcg cccggaaacc gcgtctctta tttgcgggct gccggaaaag
     4261 gttgtgcaga ctgaagagcg caaaaaagcg aatcagtttg atgcgccaat ctctatttat
     4321 gaagttcacc tgggctcctg gcgtcgccac accgacaaca atttctggtt gagctaccgc
     4381 gagctggccg atcaactggt gccttatgct aaatggatgg gctttaccca tctcgagcta
     4441 ctgcccatta acgagcatcc cttcgatggc agttggggtt atcagccaac cggcctgtat
     4501 gcaccgaccc gccgttttgg tactcgcgac gacttccgtt atttcattga tgccgcacac
     4561 gcagctggtc tgaacgtgat tctcgactgg gtgccaggcc acttcccgac cgatgacttt
     4621 gcgcttgccg aatttgatgg cacgaacttg tatgaacaca gcgatccgcg cgaaggctat
     4681 catcaggact ggaacacgct gatctacaac tatggtcgcc gtgaagtcag taacttcctt
     4741 gtcggtaacg cactttactg gattgaacgt tttggtattg atgcgctgcg cgtcgatgcg
     4801 gtggcgtcaa tgatttatcg cgactacagc cgtaaagagg gggaatggat cccgaacgaa
     4861 tttggcggtc gcgagaatct tgaagcgatt gaattcttgc gtaataccaa ccgtattctt
     4921 ggtgagcagg tttccggtgc agtgacaatg gcggaggagt ctaccgattt ccctggcgtt
     4981 tctcgtccgc aggatatggg cggtctgggc ttctggtaca agtggaacct cggctggatg
     5041 catgacaccc tggactacat gaagctcgac ccgatttatc gtcagtatca tcacgataaa
     5101 ctgaccttcg ggatgctcta caactacact gaaaacttcg tcctgccgtt gtcgcatgat
     5161 gaagtggtcc acggtaaaaa atcgattctc gaccggatgc cgggcgacgc atggcagaaa
     5221 ttcgctaacc tgcgcgccta ctacggctgg atgtgggcat tcccgggcaa gaaactactg
     5281 ttcatgggga acgaatttgc ccagggccgc gagtggaacc atgacgccag cctcgactgg
     5341 catctgttgg aaggcggcga taactggcac cacggtgtcc agcgtctggt gcgcgatctg
     5401 aaccacacct actgccacca taaagcaatg catgaactgg attttgaccc gtacggcttt
     5461 gaatggctgg tggtggatga caaagaacgc tcggtgctga tctttgtgcg tcgcgataaa
     5521 gagggtaacg aaatcatcgt tgccagtaac tttacaccgg tgccgcgtca tgattatcgc
     5581 ttcggtatta accagccggg taaatggcgt gaaatcctca ataccgattc catgcactat
     5641 cacggcagta atgcaggcaa tggcggcgca gtacacagcg atgagattgc cagccacggt
     5701 cgtcagcatt cactgagcct gacgctacca cctctggcca ctatctggct ggttcgggag
     5761 gcagaatgac acaactcgcc attggcaaac ccactcccct cggcgcgcat tacgacggtc
     5821 agggcgtcaa cttcacactt ttctccgctc atgccgagcg ggtagagctg tgtgtctttg
     5881 atgccaatgg ccaggaacat cgctatgact tgccagggca cagtggcgac atttggcacg
     5941 gttatctgcc ggatgcgcgc ccgggtttgc gttatggtta tcgcgttcat ggcccctggc
     6001 aacctgccga ggggcatcgc tttaacccgg cgaagttgtt gattgatcct tgcgcgcggc
     6061 aaatttacgg ggagtttaaa gataacccgc tgctgcacgc cggtcataat gaacctgact
     6121 atcgcgacaa cgccgccatt gcgccgaaat gcgtagtggt ggttgatcac tatgactggg
     6181 aagatgatgc cccgccgcac acgccgtggg gcagcaccat catttatgaa gcccatgtca
     6241 aaggattaac gtacctgcat ccggagatcc cagtcgagat ccgtggcact tataaagccc
     6301 tggggcatcc ggtgatgatc aactatttga aacagttggg cattaccgcg ctggaactgc
     6361 tgccggtggc acaatttgcc agcgaaccac gtctgcaacg
//
LOCUS       DAWDRC010000176         6370 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5651_length_6370_cov_2.243389, whole genome shotgun sequence.
ACCESSION   DAWDRC010000176 DAWDRC010000000
VERSION     DAWDRC010000176.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5651_length_6370_cov_2.243389"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1982..1991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2536..2545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3260..3269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4599..4608
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1481 a         1580 c         1624 g         1645 t
ORIGIN      
        1 aatcgctaac gtgatcgttc ccgcatccag atagatacaa cgtcccggct gaacaagact
       61 tgccgccagc ttgccaatag ccgctttttg cgtcattgcc agcgcagttt ttacctgatg
      121 agaaggttca tgtgccacgc gtcccggaga ctggacgccc ccggacacca acacaacggc
      181 tccctgctgc tccagttttt gtaaatcccg acgaatggtc atatgtgaca cattcattct
      241 gtccgttagt tcagcaatac taacaatgcc tttttcagct accatctcaa ggatgatttg
      301 gcgacgctct acgggtatca acttttgctc cttcctttgt cctgctgaca ttctacgcta
      361 tttgcctgcg aaacgtgcgc ggcgcaacta acgcttagtt cacataaaat aacacgcaat
      421 gttaattttt gtgaatcagc tcaccatatc gttatctttc cgcgcttata ttcacaacat
      481 caaacaaaat atcacttaaa ttaacaagga gagcagatga aaacgggatc ggagtttcat
      541 gtcggtatcg ttggcttagg gtcaatggga atgggagcag cactgtcatg tgtccgcgca
      601 ggtctttcta cctggggcgc agacctgaac agcaatgcct gcgctacgtt gaaagaggct
      661 ggtgcttgcg gagtttctga taacgccgcg acgtttgccg aaaaactgga cgctctgctg
      721 gtgctggtgg tcaatgcgac ccaggttaaa caggtgctgt ttggtgaaaa aggcgttgca
      781 caacatctga aacccggtac ggcagtaatg gtttcttcca ctatcgccag tgctgatgcg
      841 caagaaattg ctaccgctct ggctggattc ggtctggaaa tgctggatgc gccggtttct
      901 ggtggtgcgg taaaagccgc taacggtgaa atgactgtca tggcctccgg tagcgatatt
      961 gcctttgaac gactggcacc cgtgctggaa gccgttgccg gaaaagttta tcgcatcggt
     1021 tcagaaccgg gactgggttc gaccgtaaaa attatccacc agttgttagc gggcgtacat
     1081 attgctgccg gagccgaagc gatggcgctt gcagcccgtg cggggatccc gctggatgtg
     1141 atgtatgacg tcgtgaccaa tgccgccgga aattcatgga tgttcgaaaa ccggatgcgt
     1201 catgtggtgg atggcgatta caccccgcat tcagccgtcg atatttttgt taaggatctt
     1261 ggtctggttg ccgataccgc caaagccctg cacttcccgc tgccattggc ctcaacagca
     1321 ttgaatatgt tcaccagcgc cagtaacgcg ggttacggga aagaagacga tagcgcagtt
     1381 atcaagattt tctctggcat cactctaccg ggagcgaaat catgatcaag attggcgtta
     1441 tcgctgatga ttttaccggc gcgacggata tcgccagttt tctggtggaa aacggtctac
     1501 caacggtaca aattaacggt gttccaacag gtaaaatgcc ggaagcaatc gacgcactgg
     1561 tgatcagcct gaaaacgcgc tcctgtccgg tggttgaagc cacacagcaa tcgctggcgg
     1621 ctctgagctg gttgcaacag caaggttgca aacagatcta tttcaaatac tgctctactt
     1681 tcgacagtac ggcgaaaggg aatatcggcc cggttaccga tgcattaatg gatgctctcg
     1741 acacgccgtt tacggtcttc tctccggccc tgccagtcaa cggacgtacg gtttatcagg
     1801 ggtatttgtt cgtaatgaat caactgctgg ccgaatccgg gatgcgccat cacccggtaa
     1861 atcccatgac cgacagctat cttccccgtc tggttgaatc gcaatccaca gggcgctgcg
     1921 gcgtcgtttc agcacatgtt ttcgaacaag gtgtgaaagc cgttcgtcag gagctggctc
     1981 gnnnnnnnnn nctcgcttac agcaagaggg ctatcgctac gcggtgcttg atgcgctgac
     2041 cgaacaccat ctggaaattc agggggaagc cttgcgcgat gccccactgg taacgggcgg
     2101 ttctggtctg gccattggcc tggcccggca gtgggcgcaa gaaaacggta accaggctcg
     2161 cgaagcaggg cgtccgctcg ctgggcgcgg cgtagtgctc tccggttcat gctctcaaat
     2221 gaccaaccgc caggtggcac attaccgtca aattgcacca gcccgtgagg ttgatgtggc
     2281 acgctgcctc tcaaccgaaa ctctggccgc ttatgcacac gagctggcag agtgggttct
     2341 gggccaggaa agtgtacttg ctccactggt ttttgccacc gccagcactg acgcattggc
     2401 agcaattcaa cagcaatacg gtgcacaaaa agccagtcag gcagtggaaa cactgttttc
     2461 tcaactagcg gcgcggttag cagcggaagg cgtgacacgc tttattgtcg caggcggtga
     2521 gacctccggc gtagtnnnnn nnnnntgtct cactcgccct taaatctggc aacttcggtg
     2581 atgaagcctt tttttcacga gcccaaagag agtttttatc atgagcgatt tcgcaaaagt
     2641 agagcagtct ttgcgagagg agatgacgcg gattgccagt tcattctttc agcgcggata
     2701 tgcaactggt tcggctggca atctgtcgct gcttttacct gacgggaatt tactggcgac
     2761 accgacaggt tcatgcctgg gcaatctcga tccgcagcgg ctttccaaag tcaccgcgga
     2821 tggcgaatgg ttaagtggtg acaaaccctc gaaagaggtg ctctttcatc tggcgctgta
     2881 tcgcaacaat ccgcgctgta aagcggtagt gcatttgcac agtacgtggt cgacggcgct
     2941 ttcctgcctg gaagggctgg acagcaataa cgttattcgg ccgttcacac catacgtggt
     3001 gatgcggatg ggtaatgtcc cgctggtgcc ctattaccga ccgggcgata aacgcatcgc
     3061 acaggatctg gcggaactgg cagcagacaa tcaggctttt ttactggcaa atcatggccc
     3121 agtggtttgc ggtgaaagcc tgcaagaagc cgccaacaat atggaagagc tggaggaaac
     3181 ggcaaagctg atttttattc tcggtgaccg cccgatccgt tatctgaccg caggtgaaat
     3241 tgcggaatta aggagttaan nnnnnnnnnc aatgcctcgt tttgcagcta atttatccat
     3301 gatgttcacc gaagtgcctt ttattgaacg cttcgccgca gcgcgaaaag ccgggttcga
     3361 tgctgtggag tttctgtttc cctatgacta ctccccccag caaatccagc agcaactggt
     3421 gcaaaaccac ctgacgctgg cgctgttcaa taccgctccc ggagatatta atgcgggaga
     3481 atggggatta tccgcccttc ccggacgtga gcacgaagca cgcgcagaca tcgacctcgc
     3541 actggaatat gcattggcgc tcaattgtga acaagtccat gtgatgtcag gcgtcgtgcc
     3601 cgctggcgaa gatgctgaac ggtaccgggc agtatttatt gataatctcc gctacgctgc
     3661 cgaccgcttt gcgccacacg gcaagcgaat attggttgaa gcactcagcc ccggcgttaa
     3721 gcctcattat ctcttctcca gccagtatca ggcactggct attgttgaag aggttgcgcg
     3781 agataacgtg tttattcaac tagatacttt ccatgcacaa aaagtagatg gcaacctgac
     3841 acatttaatc cgcgactacg ccgggaaata tgcgcacgta caaattgccg gactacctga
     3901 tcgacatgaa ccggacgatt gagaaatcaa ctatccgtgg ctgttccgcc tgttcgatga
     3961 agtgggatat cagggctgga tcggttgtga atacaaacct cgtggcctca ccgaagaagg
     4021 gcttggctgg tttgacgcct ggcgctaatt cgctcaatcc ctaattacaa cgtacccata
     4081 catcccccca tctacggatg gggtaggtta actataactt tcagacaggg tttcccatgt
     4141 ccacaattac attgttatgc attgcgttag ctggcgtaat catgctgttg ctactggtca
     4201 tcaaggcaaa ggtacaacca ttcgttgctc tgctcctcgt cagcctgtta gtcgcacttg
     4261 cggcgggtat accggcgggc gaagtgggta aagtgatgat cgccgggatg ggcggtgtgc
     4321 ttggttccgt cactattatt attggtctgg gcgctatgct ggggcgtatg atcgaacact
     4381 ctggtggtgc agagtcactg gctaattatt tcagtcgcaa gttaggtgac aaacgaacta
     4441 tcgctgcgct gactctggca gcgttcttcc tcggtattcc cgtcttcttt gatgtcggct
     4501 ttattattct tgcgccaatc atttacggtt ttgccaaggt tgccaaaata tcaccactca
     4561 aatttggcct gcctgtcgct gggatcatgc tcactgttnn nnnnnnnntg gcggtaccgc
     4621 cgcatccagg ccctgtcgcc gcagcggggt tactccacgc agacatcggc tggctaacca
     4681 tcatcggtat tgcgatttct attcccgtag ggattgttgg ctactttgca gcgaaaataa
     4741 tcaataagcg ccaatatgcg atgtcggtcg aggtactgga acagatgcaa ctggctccgg
     4801 ccagtgagga aggcgcgaca aaattaagcg ataaaataaa tccaccgggc gtcgcgctgg
     4861 tcacctcgct aattgttatt cctatcgcga ttatcatggc gggtacggtt tctgcaacac
     4921 tgatgccgcc ttcgcatccc ctgcttggta cgctacagtt gatcggctca ccaatggtag
     4981 ctctaatgat tgcgctggtg ctggcattct ggttattggc tttacgtcgc ggctggagct
     5041 tacaacacac cagcgacatt atgggctctg cccttcccac tgcggcggta gtgattttgg
     5101 ttactggtgc tggaggggta tttggcaaag tgctggtgga atcgggcgtc ggtaaagccc
     5161 ttgccaacat gttacaaatg attgacctgc ctctgttacc agccgcattt attatttcac
     5221 tggcgctgcg tgcatcgcag gggtcagcaa ccgtagcaat cctgacaacc ggcgggttac
     5281 tctcagaagc ggtgatggga ttaaatccga ttcagtgcgt attggtgacg ctggcagcct
     5341 gctttggtgg gcttggcgcg tcacatatta atgactcagg gttctggatt gtgaccaaat
     5401 atctggggtt gtcggtagca gacggtctga aaacctggac agtgttaacg accattctcg
     5461 gttttaccgg attcttaatt acctggtgcg tatggctcgt catctaataa tccagataag
     5521 atatgatgta tcgtttaagt gcctgataag ggtgtaatgt atgccaatat taacctcggt
     5581 aatatctcgc taccgaggtc aattttcact ttgcaatata attacagtct cttgcaagtt
     5641 gattttatct tagtaataag aaccgaccaa tcttattgcc atttcgatat tagcaggcat
     5701 agatgcgtcg tagccataca tatggccata tttcggacgt aatacttcag ttactttctt
     5761 aataattgct tcgcgctgag ctcgcgcaaa atcagcatga ttatttgccg ttttattcat
     5821 aaatttacca aaactttctt cttttctgac tttaattgtc gtatcccgaa gatcattaag
     5881 aaactggcgt tgaaatttaa aatcatcgtg tgaaccaata ttaccatgcc cgccaataaa
     5941 atatttccag tcaagcattt caacctgacg caaattactg tcatggtaaa ccaacgtatc
     6001 cgatacggcg aagcccatca tcggcagttg gtcaggattc aggagatccg ggctgtgagc
     6061 tactttttcc tgttttagca accatacaga atggtcgtcg gtatgtcctg ctttaacaaa
     6121 tcggtgtaat tcaatggtct gcttttcaaa tttaaactgg tcatcttttt tacttaaaac
     6181 ctgagtggcg acgggtaagc gactgttcat aaattgcatt ttttcagcag tggctttact
     6241 ggcaataata cgcagattca cgccttcttt cttcgcctca tcgttccaaa atggactatc
     6301 gccaatgtga tccacatggt aatgagaata aaccagcgct gttacgggta atggagtcac
     6361 atcacgaatt
//
LOCUS       DAWDRC010000177         6368 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5653_length_6368_cov_2.316490, whole genome shotgun sequence.
ACCESSION   DAWDRC010000177 DAWDRC010000000
VERSION     DAWDRC010000177.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5653_length_6368_cov_2.316490"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    304..313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    697..706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1524 a         1592 c         1814 g         1418 t
ORIGIN      
        1 acataccgtc aaccaggcgt ctgccgtcag acaaaattag ctcgcaacct tcggcgcttg
       61 ccaccggata aaccggcagc ggggaggtca tggatgtgta tgggtgccag atatggcgtt
      121 ggtcaaaggt aagatcgtcc gttgtcataa tcgacttgta aaccaaattg aaaagattta
      181 ggtttacgag tctacaccga attaacaaca aaaaacacgt tttggagaag ccccatggct
      241 caccgcccac gctggacatt gtcgcaagtc acagaattat ttgaaaaacc gttgctggat
      301 ctgnnnnnnn nnnggaaacc tcattgagct tatgggagat gatgatgcag gtcatgccct
      361 gctgtttgaa gcgcagcagg tgcatcgtca gcatttcgat cctcgtcagg tgcaggtcag
      421 cactttgctg tcgattaaga ccggagcttg tccggaagat tgcaaatatt gcccgcaaag
      481 ctcgcgctac aaaaccgggc tggaagccga gcggttgatg gaagttgaac aggtgctgga
      541 gtcggcgcgc aaagcgaaag cggcgggatc gacacgtttc tgtatgggcg cggcgtggaa
      601 gaatccccac gaacgtgata tgccgtacct ggaacaaatg gtgcaggggg taaaagcgat
      661 ggggctggag gcgtgtatga cgctgggcac gttgagnnnn nnnnnntcag gcgcagggcc
      721 tggcgaatgc cgggttggat tactacaacc acaacctcga cacctcgccg gagttttacg
      781 gcaatatcat caccacgcgt acctatcagg aacgcctcga tacgctggaa aaagtgcgcg
      841 acgccgggat caaagtctgc tcaggtggca ttgtgggttt aggcgaaaca gtaaaagatc
      901 gcgccggatt attgctgcaa ctggcaaacc tgccgacgcc gccggaaagc gtgccaatca
      961 acatgctggt gaaggtgaaa ggcacaccgc ttgctgataa cgatgatgtc gatgcctttg
     1021 attttatccg taccattgcc gtagcgcgga tcatgatgcc gacctcttac gtacgccttt
     1081 ctgccggacg cgagcagatg aacgaacaga ctcaggcgat gtgctttatg gctggcgcaa
     1141 actcgatttt ctacggttgc aaactgctga ccacgccgaa tccggaagaa gataaagacc
     1201 tgcaactgtt ccgcaaactg gggctaaatc cgcagcaaac tgccgtgctg gcgggcgata
     1261 acgaacaaca gcagcgtctg gagcaggcgc tgatgacccc ggacactgac gaatattaca
     1321 acgcggcagc actatgatct ggcaggagaa aatcgacgcg gcgcttgatg cgcggcgtgt
     1381 tgctgatgcc ttgcgtcgcc gttatccggt ggcacaagga gccggacgct ggctggtggc
     1441 agatgattgc cagtatctga acttttccag taacgattat ttaggtttaa gccatcatcc
     1501 gcaaattatc cgcgcctggc agcagggggc ggaacaattt ggcgtcggta gcggcggttc
     1561 cggtcacgtc agcggttata gcgtggcgca ccaggtgctg gaagaagaac tggccgaatg
     1621 gctgggctat tcgcgggcac tgctgtttat ttctggtttt gccgctaatc aggctgtcat
     1681 tgccgcgatg atggcgaaag aggaccgtat tgttgccgac cggcttagcc atgcctcatt
     1741 gctggaggct gcaagtttaa gcccgtcgcc gcttcgccgt tttgctcata acgatgtcac
     1801 tcatctggcg cgactgcttg cttccccctg tccggggcag caactggtag tgacagaagg
     1861 tgtgttcagc atggacggcg atagtgcgcc actggaggaa atccagcagg taacgcaaca
     1921 gcacgatggc tggttgatgg tcgatgacgc ccacggcacg ggcgttatcg gggagcaggg
     1981 gcgtggcagc tgctggctgc aaaaggtaaa accagaattg ctggtggtga cgtttggcaa
     2041 aggatttggc gtcagcgggg cagcggtgct ttgctccaat acggtggcgg attatctgct
     2101 gcaattcgct cgccatctta tctacagcac cagtatgccg cccgctcagg cgcaggcatt
     2161 acgtgcgtcg ctggcggtca ttcgcagtga tgagggtgat gcacggcgcg aaaaactggc
     2221 ggcactcatt actcgttttc gtgccggagt gcaggatttg ccgtttacgc ttgctgactc
     2281 atgcagcgcc atccagccat tgatcgtcgg tgataacagc cgtgcgttac aactggcaga
     2341 aaaactgcgc cagcaaggct gttgggtcac ggcaattcgc ccaccaaccg tacccgctgg
     2401 tactgcgcga ctgcgcttaa cgctaaccgc agcgcatgaa atgcaggata tcgaccgtct
     2461 gctggaggtg ctgcatggca acggttaata aacaagccat tgctgcggca tttggacggg
     2521 cagccgcaca ctatgagcaa catgccgatc tacagcgcca gagtgctgac gccttactgg
     2581 caatgctccc acagcgtaaa tacacccacg tactggatgc gggttgtgga ccaggctgga
     2641 tgagccgccg ctggcgagag cgtcacgcgc aggtgactgc gttagatctc tctccgccaa
     2701 tgcttgttca ggcacgccag aaggatgccg cagaccatta tctggcggga gatatcgaat
     2761 ccctgccgtt agcgactgcg acgttcgatc ttgcatggag caatctcgcg gtgcagtggt
     2821 gcggtaattt atccatggca ctccgcgagc tgtatcgggt ggtgcgcccc ggaggcgtgg
     2881 tcgcgtttac cacgctggtg cagggatcgt tacccgaact gcatcaggcg tggcaggcgg
     2941 tggacgagcg cccgcatgct aatcgctttt taccgccaga taaaatcgaa cagtcgctaa
     3001 acgccgtgca ttatcaacat tatattcagc ccatcacgct gtggtttgat gatgcgctca
     3061 gtgccatgcg ttcgctgaaa ggcatcggtg ccacgcatct tcatgaaggg cgtgacccgc
     3121 gaatattaac gcgttcgcag ttgcagcgat tgcaactagc ctggccgcaa caacaggggc
     3181 gatatcctct gacgtatcat ctttttttgg gagtgattgc tcgtgagtaa atgttatttt
     3241 gtcaccggaa cggataccga agtggggaaa actgtcgcca gctgcgcgct tttacaagcc
     3301 gcaaaggcag caggctaccg gacggcaggt tataaaccgg tggcctctgg cagcgaaaag
     3361 accccgaaag gcttacgtaa tagtgacgcg ctggcgttac agcgcaacag tagcctgcag
     3421 ctggattacg caacggtaaa tccttacacc ttcgcagaac ccacctcgcc gcatatcatc
     3481 agcgcgcaag agggcaggcc gatagaagca ttggtaatga gcgccggatt acgcgcgctt
     3541 gaacaacagg ctgactgggt gttagtggaa ggtgctggcg gctggtttac gccgctttct
     3601 gacactttca cttttgcgga ttgggtaaca caggaacaac tgccagtgat actggttgtg
     3661 ggcgtgaaac tcggctgtat taatcacgcg atgttgactg cgcaggcaat actacacgcc
     3721 ggactgactc tggcggggtg ggtggcgaac gatgtcacgc ctccgggaaa acgtcacgct
     3781 gaatatatga ccacgctcac ccgcatgatt cccgcgccgc tgctgggcga aattccctgg
     3841 cttgcagaaa atccagaaaa tgcggcaacc ggaaagtaca tcaatcttgc cttaatgtag
     3901 tcatcctgtg cttggttaaa ttgtgagcga gatcgcgtct ccgattgact gcaatttaac
     3961 caattaaatt ctaaaaaaat cacgaaaaaa attttacctc cgcatcatgc ggtgaatgtg
     4021 ggaattgccc aggcggcggg ggataggggc tgcagacagt tatccactat tcctgtggat
     4081 aaccatgtgt attagagtta gaaaacacga ggcaagcgag agaatacgcg gcttgcacgc
     4141 aaattggcgt taaagacggc tcaaagaaat atcttttatt ttttaacagg ttagataatt
     4201 gcaatggcag tcactgaaca ggcatctctt gccataaaac tgtcatcaat catcttgaca
     4261 aatgttaaaa aagccgttgc tttggggata acccggtaag gccggagttt tatctcgccg
     4321 cagagtaaat cttgttcatg attgacagcg gagtttacgc tgtaacagaa atattatggt
     4381 gatgaactgt ttttttatcc agtataattt gttggcataa ttaagtacga cgagtaaaat
     4441 tacatacctg cccgcccaac tccttcaggt agcgactcat gagtaaaccg ttcaaactga
     4501 attccgcttt taaaccttct ggcgatcagc cggaggcgat tcgacgtctc gaagaggggc
     4561 tggaagatgg cctggcgcac cagacgttac ttggcgtgac tggctccggg aaaaccttca
     4621 ccattgccaa tgtcattgct gaccttcagc gcccaaccat ggtacttgcg cccaacaaaa
     4681 cgctggcggc ccagctgtat ggcgaaatga aagaattctt tccggaaaac gcggtggaat
     4741 atttcgtctc ctactacgac tactatcagc cggaggccta tgtaccgagt tccgacacct
     4801 tcattgagaa agatgcctcg gtaaacgaac atatcgaaca gatgcgtttg tccgccacca
     4861 aagcgatgct ggagcggcgt gatgtggttg tggtggcgtc tgtttccgcg atttatggtc
     4921 tgggcgatcc tgatttatat ctcaagatga tgctccatct cacggtcggt atgattatcg
     4981 atcagcgcgc gatcctgcgc cgactggcgg agctgcaata tgctcgtaat gatcaagcat
     5041 tccagcgtgg tactttccgt gttcgtggcg aggtgattga tatcttcccg gcagaatcgg
     5101 atgacattgc acttcgcgtg gagctgtttg acgaggaggt ggaacgattg tcgttatttg
     5161 acccgctgac cgggcagatt gtttccacta ttccacgttt taccatctac ccgaaaacgc
     5221 actacgtcac gccacgccag cgcatcgtcc aggcgatgga ggagatcaaa gaagagctgg
     5281 ccgccagacg taaagtgcta ttggaaaaca acaaactgct ggaagagcag cggctgaccc
     5341 agcgtaccca gtttgatctg gagatgatga acgagctagg ctactgttcg gggattgaga
     5401 actactcgcg cttcctctcc ggtcgtggac cgggtgagcc accgccgacg ctgtttgatt
     5461 acctgcctgc tgatgggctg ctggtggtcg atgaatctca cgtcaccatt ccacaaattg
     5521 gcggcatgta tcgcggtgac cgggcgcgta aagagacact ggtggagtac ggcttccgcc
     5581 tgccatcagc gctggataac cgtccgctga aatttgaaga gttcgaagca ttagcgccgc
     5641 aaaccatcta tgtttcggcg acgccgggta attacgagct ggaaaaatcc ggtggcgatg
     5701 tggtggatca ggtggtgcgt ccaacaggct tactcgaccc gattatcgaa gtgcggccag
     5761 tggcaacaca ggtggatgat cttctttcgg agattcgtca gcgagcggca attaacgaac
     5821 gcgtactggt tacaactctg accaagcgga tggcggaaga tctcactgaa tatctcgaag
     5881 aacacggtga gcgcgtgcgt tatcttcact cagatatcga caccgtcgaa cgtatggaga
     5941 ttatccgcga cttgcgtctg ggtgagttcg acgtattggt agggatcaac ttactgcgcg
     6001 aaggtctgga tatgccggag gtttcgctgg tggcgatcct cgacgctgac aaagaaggct
     6061 tcctgcgttc cgaacgttcg ttgatccaga ccattggtcg tgcggcacgt aacgttaacg
     6121 gtaaagcgat tctctacggc gataagatca ccccatcaat ggcgaaagcg attggcgaaa
     6181 ccgaacgtcg ccgcgagaaa cagcagaagt acaacgaaga acacggcatt acgccgcaag
     6241 gcttgaacaa gaaagtggtc gatatcctgg cgctggggca gaacattgcc aaaaccaaag
     6301 cgaagggcag aggaaaatcg cgcccgattg ttgagccgga taatgtgccg atggatatgt
     6361 cgcctaaa
//
LOCUS       DAWDRC010000178         6367 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5655_length_6367_cov_2.418409, whole genome shotgun sequence.
ACCESSION   DAWDRC010000178 DAWDRC010000000
VERSION     DAWDRC010000178.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5655_length_6367_cov_2.418409"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    860..869
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3776..3785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4592..4601
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5720..5729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1518 a         1562 c         1707 g         1540 t
ORIGIN      
        1 ccaccgctga acagcgcttt cagtgcgccg aagaagcggc tcaacagccc aggttgggca
       61 ggagcggctg gtgttgccgg tgcagattta ggtgctgggg ctacgacagg cgcggcaggt
      121 tcagctggcg ttggtgctgg cggcacatcc ggcatggcaa aggttgccag cgcaggttgt
      181 tccggacgct tacgttcagc gaactcttct tcagacggca gcgccatcgc ttcttcatgc
      241 agcttcggca gcatgtagct taaggttggg gtttcttccc ctttgcgcac gcgcagcaca
      301 tggtagtgcg gggtttccat ctgatcgttt ggcacaatca cacagcgtac gccgtcctga
      361 cgagtttcaa tcgcatttac cgcagaacgt ttttcattca gcaggtaaga agcaattggc
      421 acaggaacaa tggcgtgaac ttcctgggtg ttctctttca gcgcttcttc ttcgatcaga
      481 cgcagaatag agagcgacag cgattcgttg tcacgaacgg taccggtacc ggagcagcgc
      541 gggcagacgt gatggctgga ttcacccagt gatgggctca ggcgctgacg ggacatttcc
      601 agcaggccaa agcgagaaat atggctgatt tgaatacgcg cacggtcctg acgcaccgct
      661 tcacgcagac ggttttctac cgcacgctgg tggcgtacag gcgtcatgtc gatgaagtcg
      721 ataacaatca ggccgccgag gtcacgcagg cgcagctgac gagcaatctc atcggcagct
      781 tcgaggttag tgttaaacgc ggtttcttcg atatcgccgc cgcgggtcgc gcgtgcggag
      841 ttgatgtcga tggccgttan nnnnnnnnng actcgatctg cgactcgatc tggtagtggc
      901 tgaacagcgg gatctcgccg gtgtacagtt tgattttgct gctgaaatcc gggcggccta
      961 atgcagcgat atgctgacgt gccagttcga gcactttcgg gttatcgata aggatttcgc
     1021 cgatgtcctg gcgtaagtaa tcgcggaatg cgcgaacgat tacgttgctc tcctgatgaa
     1081 tcaggaacgg agccgggcgg ctttcagcgg cttttttgat ggcttcccag tgtttcagac
     1141 ggaagcttaa atcccattgc agcgcctcag cagatttgcc gacgccagcg gtgcgcacga
     1201 taagccccat gccttccggc agttcaaggc ttgccagtgc ttcttttaat tcggtacggt
     1261 cgtcgccttc gatacgacga gaaataccgc ccgcgcgcgg gttgttcggc atcagaacca
     1321 ggtagctacc cgccatactg ataaaggtgg ttaatgccgc gcctttgttg ccgcgctctt
     1381 ctttatcgat ctgaacaatg acttcctgac cttcacgcaa cacatcttta atgttgggac
     1441 gaccatgagc actgtagtta gcagggaaat attcgcgggc aatttctttt agtgggagga
     1501 aaccgtgacg ttcagcgccg taatcaacaa aagcagcttc cagactcggt tcaatgcggg
     1561 tgattttacc tttgtagatg tttgcctttt tctgctcgtg ccctggactt tcgatatcca
     1621 ggtcatacag acgctgccca tctacaaggg caacgcgcaa ctcttcctgc tgagttgcgt
     1681 tgattaacat tcttttcatc gtaacttact cattattctt acattgacga ctaagctgcg
     1741 ggcaaagtaa cgcctttccg ggtgtgaacc gatggcctcg tgtctagtcg cgtcgccaac
     1801 ctcacggtta tcgtcagctc aaagaggcgc agagtgtcgg ttgcccgttt ttcatgcgga
     1861 aaaacagcgc aattatcaaa gaaacagact gggtattact ctccagaaat atttccatct
     1921 accggtaagg actgcaaccc gcagcccgct aactgcctga aagatcaata cgtcttacgc
     1981 cattgctgcg tggatgatcg gtcgggcaaa atgggttatt ccgtaaaatt tcttgtttta
     2041 acaaggatgg acacggaaac ggcctcatta ttccactgca agccttgtta tagcaagatg
     2101 acttttacca tttatcaccc gcttactcac agttttttca cttcttgctg gtgattggtt
     2161 taataaccat caataaagtg atcacgtagt gaagtaataa ggtgcaaaac taaatataag
     2221 catagaaaaa tgagtggcgc gaatcctgtt ggctaattag aatcgcccac catgaaaaca
     2281 gagactccat ccgtaaaaat tgttgctatc accgctgacg aagcggggca acgtatcgat
     2341 aactttttgc gtacccaatt gaaaggtgta ccaaaaagta tgatttaccg tattttgcgt
     2401 aaaggcgaag tgcgggtgaa caaaaaacgt attaagcctg aatataaact cgaagcgggt
     2461 gatgaccata tcctggtgct gaataaacct tccggtacgg cggtacatgg cggtagtggt
     2521 ttaagcttcg gcgttatcga aggtttgcgg gcgttgcgcc cggaagcgcg gttccttgaa
     2581 ctggttcatc gtctcgaccg ggacacttca ggtgtgttgc tggtagcgaa aaaacgctcg
     2641 gcgttgcgtt ctctgcatga gcaattacgt gaaaaaggga tgcaaaaaga ttacctggcg
     2701 ctggtgcgcg gtcagtggca gtcgcatgtg aagagcgttc aagcgccgtt attgaaaaat
     2761 attttgcaaa gcggcgaacg tatcgtgcgt gtcagtcagg aaggcaaacc gtcggaaaca
     2821 cgctttaaag tggaagaacg ctatgcattt gccaccctgg tgcgttgtag cccggtaaca
     2881 gggcgtactc atcagatccg tgtgcataca cagtatgcgg gtcatccgat tgcctttgac
     2941 gatcgctacg gtgaccgtga atttgacaga cagctcactg aagccggcac gggattaaat
     3001 cgtctgttcc tgcacgccgc agcgttgaag tttacccatc cggggaccgg agaggtgatg
     3061 cgtatcgaag cgccgatgga tgaaggtttg aagcgttgtt tgcaaaagct gcgtaacgcg
     3121 cgctaataca agcatataaa cctgataatg ggcggttggc ctgataaggc gtttacgccg
     3181 catcaggccg ccagcaccga ttgccggatg cgacgtaacc gcatccggcg caaacaacta
     3241 tcccatcagc gggtttttcc cttcccgacg taacatctga cacagcgcga ttagcggtaa
     3301 accaaccagc gtgttaggat ctcgcccctc taaacgctca aacagcgtaa tgccaaatcc
     3361 ttcactctta aagctacccg cgcagtgcag gggatgctcc ttacgcacgt aattatcaat
     3421 ctccgcctcg ctcaggtgac ggaaatggac gtcaaaaggc tccacttctg tttgcagatg
     3481 cccattcgcc gaattaaaca acgccagtcc agtatagaag gtgacgatat tgccgctggc
     3541 tttgcgtaat tgcagacggg cattttcttc cgttaacggt ttaccggtga tttcgccatc
     3601 aagaacacat acctggtctg aaccaataat taaatgatcc ggataacgtg acgccagaga
     3661 ttgcgctttt tcttgtgcca gtcgaagcac caactgtcgc ggtgattcgt cgctgcgtgg
     3721 ggtttcgtcg acctcgggtg ctgcacattc aaaagagatt tgcagttttt ccagannnnn
     3781 nnnnntgcgc ttcgttttgc aggttgatgt tcgttatcag cactgaacga aaataaagca
     3841 gtaacccgca atgtgtgcga attattggca aaaggcaacc acaggctgcc tttttctttg
     3901 actctatgac gttacaaagt taatatgcgc gccctatgca aaaggtaaaa ttacccctga
     3961 ctctcgatcc ggttcgtacg gctcaaaaac gccttgatta ccagggtatc tatactcctg
     4021 atcaggttga gcgcgtcgcc gaatccgtag tcagtgtgga cagtgatgtg gaatgctcca
     4081 tgtcgttcgc tatcgataac caacgtctcg cagtgttaaa cggcgatgcg aaggtgacgg
     4141 taacgctcga gtgtcagcgt tgcgggaagc cgtttactca tcaggtctac acaacgtatt
     4201 gttttagtcc tgtgcgttca gacgaacagg ctgaagcact gccggaagcg tatgaaccga
     4261 ttgaggttaa cgaattcggt gaaatcgatc tgcttgcaat ggttgaagat gaaatcatcc
     4321 tcgccttgcc ggtagttccg gtgcatgatt ctgaacactg tgaagtgtcc gaagcggaca
     4381 tggtctttgg tgaactgcct gaagaagcgc aaaagccaaa cccatttgcc gtattagcca
     4441 gcttaaagcg taagtaattg gtgctccccg ttggatcggg gataaaccgt aattgaggag
     4501 taaggtccat ggccgtacaa cagaataaac caacccgttc caaacgtggc atgcgtcgtt
     4561 cccatgacgc gctgaccgca gtcaccagcc tnnnnnnnnn naccagcctg tctgtagaca
     4621 aaacttctgg tgaaaaacac ctgcgtcacc acatcactgc cgacggttac taccgcggcc
     4681 gcaaggtcat cgctaagtaa tcacgcgtct gcgtgatgaa gcttagtgag gattttcccc
     4741 aggcaactgg ggaaagacca aaccgggcgg cgacgatacc ttgacacgtc taaccctggc
     4801 gttagatgtc atgggagggg attttggccc ttccgtgaca gtgcctgcag cattgcaggc
     4861 actgaattct aattcgcaac tcactcttct tttagtcggc aatcccgacg ccatcacgcc
     4921 attacttgct aaagctgact ttgaacaacg ttcgcgtctg cagattattc ctgcgcagtc
     4981 agttatcgcc agtgatgccc ggccttcgca agctatccgc gccagtcgtg ggagttcaat
     5041 gcgcgtggcc ctggagctgg tgaaagaagg tcgagcgcaa gcctgtgtca gtgccggtaa
     5101 taccggggcg ctaatggggc tggcaaaatt attactcaag cccctggagg ggattgagcg
     5161 tccggcgctg gtgacggtat taccgcatca gcaaaagggc aaaacggtgg tcctcgattt
     5221 aggggccaac gtcgattgtg acagtacaat gttggtgcaa tttgccatta tgggctcagt
     5281 tctggctgaa gaggtggtgg aaattcctaa tcctcgcgtg gcgttgctca atattggtga
     5341 agaagaagta aagggtctcg acagtattcg ggatgcctca gcggtgctta aaacaatccc
     5401 ttctatcaat tatatcggct atcttgaagc caatgagttg ttaactggca agacagatgt
     5461 gctggtttgt gacggcttta caggaaatgt cacattaaag acgatggaag gtgttgtcag
     5521 gatgttcctt tctctgctga aatctcaggg tgaagggaaa aaacggtcgt ggtggctact
     5581 gttattaaag cgttggctac aaaagagcct gacgaggcga ttcagtcacc tcaaccccga
     5641 ccagtataac ggcgcctgtc tgttaggatt gcgcggcacg gtgataaaaa gtcatggtgc
     5701 agccaatcag cgagcttttn nnnnnnnnnc agtgcaggcg gtgcagcgac aagttcctca
     5761 gcgaattgcc gctcgcctgg aatctgtata cccagctggt tttgagctgc tggacggtgg
     5821 caaaagcgga actctgcggt agcaggacgc tgccagcgaa ctcgcagttt gcaagtgacg
     5881 gtatataacc gaaaagtgac tgagcgtaca tgtatacgaa gattattggt actggcagct
     5941 atctgcccga acaagtgcgg acaaacgccg atttggaaaa aatggtggac acctctgacg
     6001 agtggattgt cactcgtacc ggtatccgcg aacgccacat tgccgcgcca aacgaaaccg
     6061 tttcaaccat gggctttgaa gcggcgacac gcgcaattga gatggcgggc attgagaaag
     6121 accagattgg cctgatcgtt gtggcaacga cttctgctac gcacgctttc ccgagcgcag
     6181 cttgtcagat tcaaagcatg ttgggcatta aaggttgccc ggcatttgac gttgcagcag
     6241 cctgcgcagg tttcacctat gcattaagcg tagccgatca atacgtgaaa tctggggcgg
     6301 tgaagtatgc tctggtcgtc ggttccgatg tactggcgcg cacctgcgat ccaacagatc
     6361 gtgggac
//
LOCUS       DAWDRC010000179         6362 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5661_length_6362_cov_2.437292, whole genome shotgun sequence.
ACCESSION   DAWDRC010000179 DAWDRC010000000
VERSION     DAWDRC010000179.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5661_length_6362_cov_2.437292"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    884..893
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5296..5305
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1600 a         1621 c         1585 g         1536 t
ORIGIN      
        1 aatcaatatt tattcaataa aatctgatgg ttttaacttg ttaacataaa aaaacgcgcc
       61 cagctatcta ttatcctgac tcgtaaaggc caaataacaa attcattata gtgataatat
      121 tcatgcgata gtttgtttac ggcgagagat aattcgcttt ttatcaccga gcatctcccg
      181 ataaccagga ttaatcagcg caattttgat cttaacctac cgcaaaacaa ccgtgcttca
      241 tgatacaaat ggattctcat tatctgaaca atacgcagca cgtctatgac aaagggcgag
      301 ttatgcaaac tgagcaacag cgagccgtaa cacggctttg tatccagtgt ggattatttc
      361 ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct ttcctcacga ctgggtcggg
      421 cgttgggaat ggacagcgtc gagagttcta tctcttcgaa cgccatagtg ctgacaacca
      481 ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca cgatcgcggc attaatatgc
      541 atgtggtgac tgaagtccag cacattgtaa ttctggcgga gcatcatctg ctggattaca
      601 aaggcgtaga gaaacgattt agccaaattc agccattacg ttacccaaga tggctggtag
      661 ccttaatggt tggcctttcc tgcgcctgtt tctgtaaact caataaaggt ggctgggatg
      721 gtgccgtcat caccttcctg gccagtacgg ccgcgatgta tatccgccag ctgctggcac
      781 aacgtcatct tcatccacag atcaactttt gcctcaccgc tttcgccgcc accaccattt
      841 ccggattgct tttgcaactt cccactttca gcaatacccc cacnnnnnnn nnncgataca
      901 cccaccattg cgatggccgc cagcgttctg ctgctggtgc cgggctttcc gttgataaat
      961 gccgtcgccg atatgtttaa aggccacatc aataccggac tggcacgctg ggcaatcgcc
     1021 agtctgctga cactggctac ctgtgtcggc gtagtaatgg cactgacgat ttgggggcta
     1081 cgcggatggg tgtgatcgaa tttctgttag cgttggcgca ggatatgatc ctcgccgcca
     1141 ttcctgcggt cggctttgcg atggtgttca acgttcccgt tcgagcgtta cgctggtgtg
     1201 cgctgcttgg tgcgataggt catggttccc gaatgatctt aatgaccagc gggctgaata
     1261 ttgaatggtc aacctttatg gcctctatgc tggtcggcac cattggtatt caatggtcgc
     1321 gctggtatct ggcgcatccg aaagtgttta ccgtggcggc tgttatccct atgttccctg
     1381 gcatatcagc ttataccgca atgatttcgg cggtaaaaat cagccagcta ggttacagcg
     1441 aaccgttgat gattaccctg ttaaccaact ttcttacagc ttcatcgatt gttggtgcgt
     1501 tatccatcgg tctttccatt cctggattat ggttgtaccg caagcgccct cgcgtataaa
     1561 attgcctcgc ttaagcacac ggatgagaga cagcctcctc tcctccgtgt gttactataa
     1621 aagttatctc ccttctcgtt catcgttcca tatttgagaa acagtatgtc ttccagagtt
     1681 ttgaccccgg acgtcgttgg tattgacgcc ctggtacacg atcaccaaac cgttctggca
     1741 aaagctgaag gcggtgtggt tgccgtattt gacaacaacg ccccggcgtt ttatgccgtc
     1801 acgcctgcac gcctggctga actgctggcg ctggaagaaa agctggcgcg tccgggaagt
     1861 gacgtggccc tggacgatca actctatcag gaaccgcaaa ccgctcccgt tgccgtaccc
     1921 atggggaaat ttgccatgta tccggactgg caacccgatg ccgactttat ccgcctggcg
     1981 gcgctatggg gcgtggcact aagagagccg gtgaccgccg aagaactggc gtcattcatt
     2041 gcctactggc aggcggaagg caaagtcttt caccatgtgc agtggcaaca aaaactggcg
     2101 cgcagcctgc aaatcggtcg tgccagcaac ggcggactgc cgaaacgaga tgtgaatacg
     2161 gtcagtgaac ctgacagcca aattccacca ggattcagag ggtaacgatg aaaaacgttg
     2221 gcgacttgat gcaacgcctg caaaaaatga tgcctgccca tatcaaacct gctttcaaaa
     2281 cgggtgaaga actactggcc tggcagaaag aacaaggggc gatccgctcc gccgctctcg
     2341 aacgtgaaaa tcgggcgatg aaaatgcagc gcacctttaa ccgctccggt attcgtccac
     2401 tgcatcagaa ctgctccttt gagaactatc gcgttgagtg tgaaggacaa atgaatgcgt
     2461 taagcaaagc gcgccagtat gtcgaggagt ttgacggcaa catcgccagc ttcatctttt
     2521 ctggtaagcc aggaaccggc aaaaaccatc tggcggcggc aatctgcaac gagctgctgc
     2581 tacgcggtaa atccgtattg atcatcaccg tggccgatat tatgtcggcg atgaaagata
     2641 ccttcaggaa tagcggtacc agcgaagaac aactgcttaa cgatctgagt aacgttgatc
     2701 tgctggtgat cgatgagatc ggcgtgcaga ccgaatcgaa atacgaaaaa gtgatcatta
     2761 accagatcgt cgatcgccgc tcttcttcca aacgcccaac cgggatgctg accaacagta
     2821 atatggaaga gatgaccaag ttgctgggcg aacgcgtgat ggaccgtatg cgcctgggta
     2881 acagtttgtg ggtgatcttc aactgggata gctaccgtag ccgtgtaaca ggtaaagagt
     2941 attaatcccc gtttctggcg gtatactgcg acacaattca accttgagta atgatgatga
     3001 aaactattaa acatcttctg tgctgtgcca ttgccgccag cgcattaatt tccaccgggg
     3061 tgcatgctgc gtcctggaaa gatgcgctct ccagcgccgc cagcgaactt ggcaaccaaa
     3121 acaacacgac acaggaaggc ggttggtcgc tcgcgtcatt aactaacttg cttagcagcg
     3181 ggaaccaggc cttaagcgca gataacatga acaacgccgc aggcattctg caatactgcg
     3241 cgaagcaaaa gctggcttcg gtaaccgatg ccgaaaacat caagaatcag gtactggaaa
     3301 aactgggcct gaacagtgaa gagcaaaaag aagacaccaa ctatctggat ggcattcagg
     3361 gtttgctgaa aaccaaagat ggtcagcaac tcaatctgga taatattgga acgactccgc
     3421 tggcagaaaa ggtgaaaacc aaagcctgcg atctggtgtt aaagcagggg ctgaacttca
     3481 tttcctgaac cgcgaaacgc cgcgttttag ctgttacccg gctgacgcgg ctctctcctg
     3541 cccttttccc tgctcctgga cggttttacc cctgttacac gggaagcgac cgcgatcaaa
     3601 aaagtctgtt tataaactaa ttcctaacaa catcgcattt ttttgacact agaattgcag
     3661 caatatggcg acgccattac attgtgcttc tcaaaaaact taaacgttgg ctaaacggcc
     3721 cggtttagtg aggatcgccc gttgtcagaa ctactctctt tcgccctttt tctcgcctct
     3781 gtgctgattt acgcatggaa agcgggacgt aacacctggt ggtttacggc cacattaacg
     3841 gtactggggc tatttgtcgt tttaaatatc accctgtttg ccagcgacta ttttactggc
     3901 gatggtatta acgacgcggt tctctatacc ttaaccaaca gcctgaccgg tgctggtgtc
     3961 agcaaataca tcctgcctgg tatcggcatt gtgctggggc tggcggcggt gttcggtgcg
     4021 ctgggctgga tcctgcgccg tcgtcgccat catccgcacc attttggtta cagcctgctg
     4081 gcactcttac tggcgctggg ttcagtggat gccagcccgg catttcgtca gataacggaa
     4141 ctggtgaaat cccagtcacg cgacggcgac ccggactttg cggcttatta taaagagccg
     4201 tcgagaacta tccctgaccc gaaactcaac ctggtttata tctacggcga aagtctcgag
     4261 cggacctatt ttgataacga ggctttcccg gatctcacgc ctgaactggg cgcgttgaaa
     4321 aatgaaggcc tggatttcag ccacacgcag cagctgccgg gaacggatta cacgattgcg
     4381 ggcatggtag cttctcagtg cggcataccg ctgtttgccc catttgaagg caacgcctcc
     4441 gcctctgtct ccagcttctt cccgcagaac atctgtctgg gcgatatcct gaaaaactcg
     4501 ggttatcaga actatttcgt gcagggcgcg aatctgcgtt ttgccggtaa agatgtgttc
     4561 ctgaagtcgc atggcttcga ccacttatac ggctcagaag agctgaaaag tgtggtggcc
     4621 gacccgcact atcgcaacga ctggggattc tacgacgata ccgttctcga tgaagcgtgg
     4681 aaaaaatttg aagagctttc ccgctcaggt cagcgattct cactgtttac cctgacagtc
     4741 gatacccatc acccggatgg ttttatctcg cgcacctgta accgcaaaaa atatgatttt
     4801 gacggtaagc cgaatcagtc attcagcgcg gtaagttgca gccaggagaa tattgcgacg
     4861 tttatcaaca aaatcaaagc gtcaccgtgg tttaaagata ccgtcatcgt cgtctcttct
     4921 gaccatttag cgatgaacaa tacggcgtgg aaatacctta ataagcagga tcgcaataac
     4981 ctgttttttg tcatccgtgg cgacaagccg cagcaagaga cgctggcagt gaagcgtaat
     5041 acgatggata acggcgcgac ggtgctggac attctcggtg gcgataacta tctcggactt
     5101 ggccgtagca gtttatccgg gcagtcgatg tcggaaatct tcctcaatat caaagagaaa
     5161 acattggcgt ggaagccgga tatcatccgc ctgtggaaat tccctaaaga gatgaaagag
     5221 ttcaccatcg accagcagaa aaacatgatt gccttctcgg gtagccattt ccgtttgccg
     5281 ctgctgttgc gggttnnnnn nnnnnaccgt tgctacaaga tggcacaact ctgggctccg
     5341 gaactggcac tctccaccga ctggtgtgtc tcgcaagggc agcttggcgg tcagcaaatt
     5401 gttcagcatg ttgacaaagc aatatggaag ggcaaaacgg catttaaaga tacggtcatc
     5461 gacatggcgc gttacaaagg caatgtcgat acgctaaaga ttgttgataa cgatattcgc
     5521 tacaaagccg acagtttcat ctttaacgtc gctggtgcgc cagaagaggt gaaacagttt
     5581 agcgggattt ctcgtccaga gtcgtggggc cgctggtcca acgcacagct gggcgatgaa
     5641 gtaaaaatcg agtacaagca tccgctgccg aagaaatttg acctggtgat taccgccaaa
     5701 gcatacggca ataacgccag ccgtcctatt ccggtacgcg taggtaatga agaacaagcc
     5761 cttgtgctgg gcaatgaagt gaccaccacc acactgcatt tcgataaccc aaccgatgcc
     5821 gacacactgg taattgtgcc gccggaacct gtctcaacca acgaagggaa tatcctcgga
     5881 cactcgccgc gtaagctcgg gatcggcatg gtggaaatta aagtggtgga acgtgaaggg
     5941 taattcctga ctgatgtgta ggtcgcataa ggtgcgacct acacaccttc tccggcctac
     6001 aaaacattat tgagaaacag aaaaaaaaca gcgcccaaca cttccccgga cgctgcatgg
     6061 caactttaat aatctcgcct tatcagaaag taattacgcc cttaatcaac tcacgattgt
     6121 taatcacatc gcgctcgtag gtttctgcca gcgtggcgaa cggatagcgg tgggttaaca
     6181 tcatgtcggc agtgattttc ccttccgcca tcaatcgacc gactttagcg aaatcttccg
     6241 gcgtggcgtt gcggctgccc atcatcgtcg tttctttttt atggaattcc ggatcggaga
     6301 actgcaactc acctttaaac aggccgacaa ataccaccgt gccgccgtga cgaatcagat
     6361 tt
//
LOCUS       DAWDRC010000180         6310 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5707_length_6310_cov_2.876099, whole genome shotgun sequence.
ACCESSION   DAWDRC010000180 DAWDRC010000000
VERSION     DAWDRC010000180.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5707_length_6310_cov_2.876099"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4140..4149
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1474 a         1582 c         1608 g         1636 t
ORIGIN      
        1 tccgtcccga acgtcaggaa gcgctcttcc gacgattaca ctcatggcgt aatcgatgta
       61 ggagttcttc atttcgtcat ggatctcatg ctgtatcatt ttctgatctt caatcaggtt
      121 actcatctat tcaaactcct cccatctata catcaagtgt tgccagcgca gcgttttctt
      181 caatgaattg cttaacagca tttgctgtca attgagacaa ctcattcccc ggagccctac
      241 aaaccatctg attcttccca tacccaccaa ttaatcccgg atcggtaggt ccataaaccg
      301 tgatattggg tctatccagt gccgccgtta aatggcttaa ccccgtatcc accgacacta
      361 caaatttagc cccagccagt acgcgggcaa cgccttccag actcatcttc ggcaatactt
      421 caacataagc aaatccttcc gccagtcgtt tcgcccgttc ttcctcatgc ggcgcgcccc
      481 acggaagttt aatccgtatt cctgaatcag ccagtaaacc aatcaattct cgccagtgtt
      541 cttccggcca gtgtttatca tctcgggttg ttgcatgaag aaatacggca tattcgccag
      601 catctgtagg caggttcgtc aggaaatgct gtgcgatagc ataatcgccc tgggtttgtg
      661 gtttgctata gcccagactt ttggcaaaca gttcgcgggt gcgttctacg gcgtgctgct
      721 gttttgcaat atgatgttta cgattgtaaa acaggctggc taacggttcg cgagcggttt
      781 gccagtccat tccatgcttt acgccatgcg ccagacgcgt caccagcgtc gcgcttttta
      841 ccagcccctg agcgtcgata actgcgtcat agttctttgc ttgtagcgct tcacgaaacg
      901 ctttgcgttc agcttttatg ggagccgaga accaggcttt acgccagcga cgtattgcca
      961 caggaataac tcgctcaacg gcagcgtgcc aggaaggaat ctgtgcgaac ccttcttcca
     1021 ccacccagtc aaacttaatc cctgggattg cctgctgggc atcagtgagt gcgggcagcg
     1081 tgtggagaac atcgcccatc gacgatgttt taacgatcag aacccgcatc cgtcaggctt
     1141 cctcttgtaa caatagcgcg ttgagttctt ccagtacgcg ctggggagta atgtcgatca
     1201 agctctggtg ataaccctcc gcagcgtcac ctttacgcac tttgtgatag ccggtaatca
     1261 ggcggatcac gcgtgcttta tgggatagcg gtggtgtgaa gtccgggctg cttggtccat
     1321 acagggcaac cagcggacga ttgagcgccg ccgcaacgtg catcagacca gaatcgttag
     1381 taacaatggc tttacaggct gcaatcagga taaccgcttg atcaagctgt gtttctcccg
     1441 ccagattccg acaccatgcc tgctgctctg tattcaaagc ggcaagaatc tcattgcccg
     1501 cctcatgatc tttcgccgag ccaaacagaa ccacctgata accttcatca atcagctgct
     1561 ttgccagctc cgcatagtgg tagtgtggcc agcgttttgc cggaccaaac tccgcacccg
     1621 ggcaaaaacc aatcatcgga cgttctgatg aaagcgaaaa ttgattacag gtatatgatt
     1681 tttcaccttc gctcacctgc aactgcggcc ataacaatgg ctgcggcaga tcttgtgcgg
     1741 tacgcataat acctttgtca taggccagcg cgacatagcg ttccaccatt agcggccagg
     1801 cttctttatc gagcacgcgt acatcgttga gtaaaccgta gcgcatctcg ccgcgccagc
     1861 cggcggcgag atgcgctacg gtttactatg acccagtttg cggcgttcgc cgatttccag
     1921 cgctccgtga ccgagaggca tagggatcgc ttcgttaact tccggcatcc gcgataataa
     1981 tggacggcac catgccggtg ccatcacgtc gattatcgcc tggggatagc gcgcctggag
     2041 cgtgcgatag agactttgcg acatcatcat gtcgccaacc caagacgggc cgatcaccag
     2101 tattttcatg cagagctctt atgcgtcacg attcagccag gccatgtatt ccgttacgcc
     2161 ttcggcaacg gttttgaacg gtttatcgta acccgccgcg cgcagattcg tcagatctgc
     2221 ctgagtgaac gcctggtagc ggcctttcag tttatccggg aacggaatgt attcgatttg
     2281 gcctttcttg tgataagcaa gcgtagcatc tgctactgcc tggaaggatt ccgcacgacc
     2341 ggtgccgagg ttgaagatgc cggaaacgcc attttccagg aaccacaggt ttacatctgc
     2401 cacgtcgccc acatagacga agtcacgttt aaagttctcg ctaccttcga acagcttcgg
     2461 tgattcaccg ttattaagct gggtgttgag atggaaagcg acgctcgcca tgctgccttt
     2521 atggccttca cgcggtccat aaacgttgaa ataacggaag ccaacaatct gtgagttcgc
     2581 ttcaggcagg atttggcgaa catattcatc aaacaggaat tttgagtaac cgtagacatt
     2641 caacggtttt tcatattcgc gggattcaat aaagtcggag gtgcgtccac cgtaagtggc
     2701 tgcggaagag gcatacagga acgggatttc gcgctccaga cagtagtgca gcagctcttt
     2761 ggagtattga tagttgttat ccatcatata cttgccgtcc cactcggtgg tggaagagca
     2821 cgcgccttcg tggaaaatcg cttcgacatc gccgaactct tcgccagcca taatctggat
     2881 caggaagtct tccttatcca tatagtccgc gatatccaga tccaccaggt tcacaaactt
     2941 ggtgccgtct ttcaggttgt ccaccaccag aatatcggtg atgcctttat cattcagggc
     3001 tttaacgatg ttgcttccga taaagcccgc gccgccggta acgatgatca taactgtaac
     3061 cttcgaatta tggagtcaga gacaatctca gacacgaata cttctatcat atcactacac
     3121 ggcgttggct tcagccattc gccaaaagtg agattgatgg acgttattta tcctgaattt
     3181 tgcagaagtg ttaacgcgtc atctcgtcgc gacctataag tttgggtaat atgtgctgga
     3241 atttgccctg tctggagaat cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat
     3301 ctggaaaccg cacgggcgga agggttgttt aaagaagagc gcattattac gtctgcgcag
     3361 caagcagata tcactgtggc tgatggaagc cacgtcatta acttttgtgc caacaactat
     3421 ctcggactgg cgaatcatcc ggatctgatt gcggcggcaa aggcgggaat ggattctcac
     3481 ggtttcggca tggcttcggt gcgttttatt tgcggcactc aggacagcca taaagagctt
     3541 gaacaaaaac tggcggcatt cctggggatg gaagatgcga ttctctactc ttcctgcttt
     3601 gatgctaacg gcggcctgtt tgaaacgctg ctgagcgcgg aggacgccat tatctccgac
     3661 gcactgaacc acgcgtctat tattgatggt gtgcgtctgt gtaaagctaa acgctatcgt
     3721 tatgccaaca acgatatgca ggagctggaa gcacgtctga aagaagcgcg tgaagccggt
     3781 gcgcgtcatg tgctgattgc caccgatggt gtgttctcga tggacggcgt gattgccaac
     3841 ctgaagggcg tttgcgatct ggcagataaa tacgatgccc tggtgatggt agacgactcc
     3901 cacgcggtcg gttttgtcgg tgaaaatggt cgtggttccc atgaatactg cgatgtgatg
     3961 gggcgtgtag acatcattac cggtacgttg ggtaaagcat tgggcggggc ttctggtggt
     4021 tataccgcgg cgcgcaaaga agtggttgag tggctgcgcc agcgttctcg tccgtacctg
     4081 ttctccaact cgctggcacc agcgattgtc gccgcatcca tcaaagtact ggagatggtn
     4141 nnnnnnnnng agccgatcac gccattattc cggtcatgct tggcgatgcg gtagtggcgc
     4201 agaaatttgc ccgtgagctg caaaaagagg gcatttacgt caccggtttc ttctatccgg
     4261 tcgttcctaa aggtcaggcg cgtattcgta cccagatgtc tgcggcgcat acccctgagc
     4321 aaattacgcg tgcagtagaa gcattcacgc gtattggtaa acaactgggc gttatcgcct
     4381 gagggtgtga gatgaaagcg ttatccaaac tgaaagcgga agagggcatc tggatgaccg
     4441 acgttcctgt accggaactc gggcataacg atctgctgat taaaatccgt aaaacagcca
     4501 tctgcgggac tgacgttcac atctataact gggatgagtg gtcgcaaaaa accatcccgg
     4561 tgccgatggt cgtgggccat gaatatgtcg gtgaagtggt aggtattggt caggaagtga
     4621 aaggcttcaa gattggcgat cgcgtttctg gcgaaggcca tattacctgc ggtcactgtc
     4681 gcaactgtcg tggcggtcgt acccatctgt gccgcaacac gatcggcgtc ggcgttaacc
     4741 gtccgggctg cttcgccgag tatctggtga ttccggcgtt caacgccttc aaaatcccgg
     4801 acaatatctc cgacgacctg gcttccattt ttgatccctt cggtaacgcc gtgcataccg
     4861 cgctgtcgtt cgatctggtt ggcgaggatg tgctggtctc tggtgcaggt ccgataggta
     4921 ttatggctgc ggcggtggcg aaacacgttg gtgcacgcaa tgtggtgatc actgatgtta
     4981 acgaataccg ccttgagctg gcacgcaaaa tgggtatcac ccgtgcggtt aacgtcgcta
     5041 aagaaaacct taatgatgtg atgacagaac tgggcatgac cgaaggcttt gatgtcggtc
     5101 tggaaatgtc cggtgcgccg ccagcgtttc gtaccatgct cgacaccatg aaccacggcg
     5161 gtcgtattgc gatgctgggt attccaccgt ccgatatgtc tatcgactgg accaaagtaa
     5221 tctttaaagg cttgttcatt aaaggtattt acggtcgtga gatgtttgaa acctggtaca
     5281 agatggcggc gctgattcag tctggcctcg atctctcgcc gatcattacc catcgtttct
     5341 ctatcgatga tttccagaag ggctttgacg ctatgcgttc gggccagtcc gggaaagtta
     5401 ttctgagttg ggattaacac gaacaagggc tggtattcca gcccttttgc ctgaggataa
     5461 tccgttaaat atgtaaaatc ctgtcagtgt aatcaagata tcgtaattgc gctgatctct
     5521 tatatagctg ctctcattat ctctctaccc tgacgtgact ctctcaccgg taaaaataat
     5581 atctcacagg cttaatagtt tcttaataca aagcctgtaa aacgtcagga taacttcaga
     5641 ggtcgtcggt aatttatgat gaacagcacc aataaactta gtgttattat tccgttatat
     5701 aatgcgggcg atgatttccg cacttgtatg gaatctttaa ttacgcaaac atggactgct
     5761 ctggaaatca ttattattaa cgatggttca acggataatt ctgttgaaat agcaaagcat
     5821 tacgcagaaa actatccgca cgttcgtttg ttgcatcagg cgaattctgg cgcatcggtg
     5881 gcgcgtaatc gtgggattga agtggcaacg ggaaaatatg tcgcttttgt cgatgctgac
     5941 gatgaggtct atcccaccat gtacgaaacg ctgatgacca tggcgttaga ggacgacctc
     6001 gacgtggcgc agtgcaacgc tgattggtgt tttcgtgaaa cgggtgaaac ctggcaatcc
     6061 atccctaccg atcgccttcg ctcaaccggt gtattaactg gtccggactg gctgcggatg
     6121 ggactttctt cccgccgttg gacacacgtg gtctggatgg gggtttatcg ccgcgatgtt
     6181 attgttaaaa ataacattaa atttattgcc ggattacatc atcaggatat tgtctggaca
     6241 acagaattta tgtttaacgc gctgcgtgcg cgatataccg agcaatcatt atataaatat
     6301 tatctgcata
//
LOCUS       DAWDRC010000181         6284 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5729_length_6284_cov_2.090384, whole genome shotgun sequence.
ACCESSION   DAWDRC010000181 DAWDRC010000000
VERSION     DAWDRC010000181.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6284)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5729_length_6284_cov_2.090384"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    832..841
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2265..2274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5595..5604
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1512 a         1534 c         1674 g         1534 t
ORIGIN      
        1 tatgacctta atttgcattt aatatcaaag ggattataac gatatggcac gagttaacct
       61 gttgacattc aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagagggaga
      121 aaattaacgg tcagttctca cgctataacc aaattgttca gctaagaaag aaaaccagag
      181 acgctaatcg aagggcgaac acaacggcat tgttatgttt atttatatag ttagttttat
      241 agctaaaatt gaaattataa aaataaataa tgaattagca ggagcatcaa cagtaatatc
      301 ttaatctctg gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact
      361 tattttgctt cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg
      421 ggttcatcaa acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg
      481 catcccacgc tttattcatc gccgcgaagt cgtttttatc agccaggaaa atggtggcat
      541 ccagaatgct cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgcgccagcg
      601 tgttggcggt ttggtcaaaa gcatcggcgt cgaggttttc cggtacacca gtgtagtaga
      661 gcgtgttgtt gtggatcacc acatcagacc agcgggcttc agcatcgata cgaacgatag
      721 tcatcatttt gtcctcatta tccttccatt ttcgtgctgc aaagactgcc acaatgtgag
      781 gtcgccgtca ccacttcaac tggcgaaagc gccctcatcc tgacataatc cnnnnnnnnn
      841 ngtacgggca aaacctacgc ttacctggcc cctgcgctgc gggcgaaaaa gaaagtcatt
      901 atctcgaccg gttcaaaagc gttgcaggat cagctctaca gccgcgattt gccaacggtt
      961 tcaaaggcgt tgaaatatac gggcaacgtg gcgttgctga aagggcgctc aaactacctc
     1021 tgcctcgaac gtctcgaaca acaggcgcta gcgggcggcg atctgccggt acaaatatta
     1081 agcgatgtga tcctgctgcg ctcctggtct aatcaaacag tcgatggtga tatcagcacc
     1141 tgcgtcagcg tggcggaaga ttcacaggcg tggccgctgg tcaccagcac caacgataac
     1201 tgccttggca gcgactgccc gatgtataaa gattgctttg tagtcaaagc acgcaaaaaa
     1261 gcgatggacg ccgatgtggt ggtggtaaac catcatctct ttctggcgga tatggtggtg
     1321 aaagagagtg gatttggcga actgatcccg gaagctgacg tcatgatctt cgacgaagcc
     1381 caccagctgc ccgacattgc cagccagtat tttggtcagt cactctccag tcgacaactg
     1441 ctcgacctgg caaaagacat caccatcgcc taccgcaccg aattaaaaga cacccagcag
     1501 ttacaaaagt gcgcggatcg tcttgcccag agtgcgcagg attttcgcct gcaactcggt
     1561 gagcctggtt atcgcggtaa cctgcgtgag ctgttagcta atccgcaaat tcagcgggca
     1621 tttttactgc tcgatgacac tctggaactt tgttatgacg tggcgaaact gtcgctgggg
     1681 cgttccgcct tgctggatgc ggcatttgaa cgcgccacgt tatatcgcac acggctgaaa
     1741 cggctaaaag agatcaatca gccgggctac agctactggt acgaatgcac ttcgcgccat
     1801 tttactctgg ctctcacgcc gctcagcgtg gcggataaat tcaaagagtt aatggcgcaa
     1861 aacccggtag ctggatcttt acctcagcaa cgctgtcggt gaacgacgat ctgcatcatt
     1921 tcacctcgcg gcttggcatt gaactggcag agtcgttgct gttacccagc ccctttgatt
     1981 acagccgcca ggcgttactc tgtgtgccgc gcaacctgcc gcaaactaat caaccgggtt
     2041 ccgcacggca actggcggca atgctgcgac cgatcatcga agctaacaac ggtcgttgtt
     2101 ttatgctttg tacctcgcac gccatgatgc gcgatctggc cgagcagttc cgcgctacca
     2161 tgacgcttcc cgttttgttg cagggggaaa ccagcaaagg gcaactgttg cagcaatttg
     2221 tcagcgccgg taacgcgctt cttgtggcaa ccagcagttt ctggnnnnnn nnnnaagggg
     2281 tggacgtgcg tggcgataca ttgtcattgg tgattatcga caaattgccg tttacctcgc
     2341 cggatgatcc actattaaaa gcacgcatgg aagattgccg tttgcgcggt ggtgatccgt
     2401 tcgatgaagt acaactgccg gatgcggtca ttactctcaa gcagggggta gggcgattga
     2461 ttcgcgacgc cgacgatcgc ggcgtgttag tgatttgcga caatcggctg gtgatgcgcc
     2521 cttacggcgc gacgtttctc gccagtctgc cgccagcgcc gcgcacccgt gacattgccc
     2581 gtgcggttcg tttccttgcg ataccatcct ccaggtaatt tgttacggag tatggtatga
     2641 tgcgcgcctt tttattgatc taagcacgaa gacccatgcg aattctggct atcgataccg
     2701 cgacagaggc ctgctctgtc gccctgtgga acgacggtac tgtcaacgct cattttgagc
     2761 tttgccctcg tgaacatact caacgaatct taccgatggt gcaggatatc ctgaccacca
     2821 gcggaacttc cctgactgac ataaacgctc tggcttacgg gcgcggcccc ggtagcttca
     2881 ccggcgtgcg cattggtatt ggcattgcgc aaggactggc acttggcgcg gagttaccaa
     2941 tgattggcgt ctccacgcta atgacgatgg cgcaaggggc gtggcgcaaa aacggcgcaa
     3001 cccgcgtgct ggcagctatt gacgcgcgaa tgggcgaagt ttactgggcc gaatatcagc
     3061 gtgatgaaaa cggtatctgg cacggtgaag aaaccgaagc cgtactcaaa cccgaactcg
     3121 ttcatgaacg aatgcaacag ctttccggcg aatgggtgac agtagggacg ggctggcaag
     3181 cctggccgga tctcggtaaa gagagcgggc tggttttgct cgatggcgaa gtgttactgc
     3241 ccgctgcaga agatatgctg ccgattgcgt gccagatgtt tgccgagggt aaaatggtgg
     3301 cggtggaaca tgctgaacca gtttatttac gtaacaacgt cgcatggaag aaacttccgg
     3361 gcaaagaatg aatcttaaga gtatgccctg agaaaaaaag gagtcgcatc atggcggttc
     3421 aaaagaatgt tatcaaaggc atactggcag gtacgtttgc gctaatgctg agcggttgtg
     3481 ttactgtgcc agacgccatt aaaggcagca gtcccacgcc gcaacaagat ttagttcggg
     3541 tgatgagtgc gccgcagctg tacgttggtc aggaggctcg ctttggcggc aaagtggttg
     3601 cggtacaaaa ccagcaaggg aaaacacgcc tggaaattgc caccgtacca ctggacagcg
     3661 gagccagacc gacgctggga gaaccttctc gcggtcgcat ttatgccgat gtgaacggtt
     3721 ttctggaccc ggtggatttc cgcggacaac tggtgacggt agtcgggcca atcaccggtg
     3781 cggttgacgg caaaatcggt aatacgccct ataaatttat ggtgatgcaa gtaacgggtt
     3841 acaaacgttg gcatttaacc cagcaggtga ttatgccgcc tcagccgatt gatccatggt
     3901 tttatggcgg tcgtggctgg ccctatggct acggcggatg gggctggtat aatcccggcc
     3961 ccgcgagagt acaaacagtt gtaactgaat aattgcttgt ttttaaagaa aaagaaacag
     4021 cggccggtcc gctgtttctg cattcttacg gtaaagataa aaataaatag tgacgcgctt
     4081 cgcaaccttt tccttgggta attatcaagc tggtaagatg agttaatatt atgttaacag
     4141 tatgtttatc acttggggtt gcgatgacga cgaacacgca ttttagaggt gaagaattga
     4201 agaaggtttg gcttaaccgt tatcccgcgg acgttccgac ggagatcaac cctgaccgtt
     4261 atcaatctct ggtagatatg tttgagcagt cggtcgcgcg ctacgccgat caacccgcgt
     4321 ttgtgaatat gggggaggtg atgaccttcc gcaaactgga agaacgcagt cgcgcgtttg
     4381 ccgcttattt gcaacaaggg ttggggctga agaaaggcga tcgcgttgcg ttgatgatgc
     4441 ctaacttatt gcaatatccg gtggcgctgt ttggcatttt gcgtgccggg atgatcgtcg
     4501 taaacgttaa cccgttgtat accccgcgtg agcttgagca tcagcttaac gatagcggcg
     4561 catcggcgat tgttatcgtg tctaactttg cccacactct ggaaaaagtg gttgataaaa
     4621 ccgccgttca gcacgttatt ctgacccgta tgggcgatca gctatctacg gcaaaaggca
     4681 cggtagtcaa tttcgttgtt aaatacatca agcgtctggt gccgaaatac catctgccag
     4741 atgccatttc atttcgtagc gccctgcaca acggctaccg gatgcagtac gtcaaaccag
     4801 aactggtgcc ggaagattta gctttcctgc aatacaccgg cggcaccact ggtgtggcga
     4861 aaggcgcgat gctgactcac cgcaatatgc tggcgaacct ggaacaggtt aacgcgacct
     4921 atggtccgct gttgcatccg ggcaaagagc tggtggtgac ggcgctgccg ctgtatcaca
     4981 tttttgccct gaccattaac tgcctgctgt ttatcgaact gggggggcag aacctgctta
     5041 tcactaaccc gcgcgatatt ccagggctgg taaaagagtt agcgaaatat ccgtttaccg
     5101 ctatcactgg cgttaacacc ttgttcaatg cgttgctgaa caataaagag ttccagcagc
     5161 tggatttctc cagtctgcat ctttccgcag gcggtgggat gcctgttcag caagtggtgg
     5221 cagaacgttg ggttaaactg actggacagt atctgctgga aggttatggc ctgaccgagt
     5281 gcgcgccgct ggtcagcgtt aacccgtatg atattgatta tcatagtggt agcatcggtt
     5341 taccggtgcc gtcgacggaa gccaaactgg tggatgatga tgataatgaa gtaccacctg
     5401 gtcaaccagg tgagctttgt gtcaaaggac cgcaggtgat gctgggttac tggcagcgtc
     5461 cggatgctac cgatgaaatc atcaaaaatg gctggttaca caccggcgac atcgcggtga
     5521 tggatgaaga aggattcctg cgcattgtcg atcgtaaaaa agacatgatt ctggtttccg
     5581 gttttaacgt ttatnnnnnn nnnntgtcgt catgcagcat cctggcgtac aggaagtcgc
     5641 ggctgttggc gtaccttccg gctccagtgg tgaagcggtg aaaatcttcg tagtgaaaaa
     5701 agatccatcg cttaccgaag agtcactggt gaccttttgc cgccgtcagc tcacgggcta
     5761 caaagtaccg aagctggtgg agtttcgtga tgagttaccg aaatctaacg tcggaaaaat
     5821 tttgcgacga gaattacgtg acgaagcgcg cggcaaagtg gacaataaag cctgagcgtt
     5881 aagtcagtcg tcagacgccg gttaatccgg cgtttttttt gacgcccact aaagagaata
     5941 caatttgaat taccaaatga ttaccacgga cgatgcgctg gcttctttgt gtgaagccgt
     6001 ccgtgccttt ccggcgatag ccctggatac tgaatttgtt cgtacgcgca cttattaccc
     6061 gcagctgggt ttaattcaac tttttgatgg tgagcatctg gcgctaattg atcctctcgg
     6121 gatcaccgac tggtcaccgc tgaaagcgat cctgcgcgat ccgtccatca caaaatttct
     6181 tcatgcaggc agtgaagatc tggaagtgtt cctcaatgtc tttggcgaat taccacaacc
     6241 cttgattgac acgcaaatcc ttgctgcctt ctgcggacgc ccga
//
LOCUS       DAWDRC010000182         6272 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5737_length_6272_cov_2.791218, whole genome shotgun sequence.
ACCESSION   DAWDRC010000182 DAWDRC010000000
VERSION     DAWDRC010000182.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5737_length_6272_cov_2.791218"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3059..3068
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1421 a         1585 c         1699 g         1557 t
ORIGIN      
        1 gaacaaacaa accagcgtat ttgcgccgaa cgtgctgatt cttgatcaga acatgacccc
       61 atcagccttc ttccccagca gttatttcac ctaccaggaa cccggcgtga tgagtgcaga
      121 tcggctggaa ggcgttatgc gcctgacacc ggcgttgggg cagcaaaaac tttatgttct
      181 ggtctttacc acggaaaaag atctccagca gacgactcaa ctgctcgacc cggctaaagc
      241 ctatgccaaa ggcgtcggta actcgatccc ggatattccc gatccggttg ctcgtcatac
      301 caccgatggc ttactgaaac tgaaagtgaa aacgaactcc agctccagcg tgttggtagg
      361 accactattt ggttcttccg ctccagctcc ggttacggta ggcaacacgg cggcaccagc
      421 tgtggctgca cccgctccgg caccggtgaa gaaaagcgag ccgatgctca acgacacgga
      481 aagttatttt aataccgcga tcaaaaacgc tgtcgcgaaa ggtgatgttg ataaggcgtt
      541 aaaactgctt gatgaagctg aacgcctggg gtcgacatct gcccgttcca cctttatcag
      601 cagtgtaaaa ggcaaggggt aattacgccc cacagtgctg attttgcaac aactggtgcg
      661 tctcctggcg cacctttttt tctcctcctg tactattgtt gcgtttttgt tgcgcattag
      721 ttcacttaat ttgctttaca tctcccgtaa atatttttct gcgatacaat gcctttacgt
      781 tatgtaacgg agagttcggc atgtcacacc ccgcgttaac gcaactgcgt gcgctgcgct
      841 attttacaga gatccctgcc ctggagccgc aactgctcga ctggctgttg ctggaagatt
      901 ccatgacaaa acgttttgaa cagcagggaa aaacggtgag cgtgacgatg atccgcgaag
      961 ggtttgtcga gcagaatgaa atccccgaag aactgccact gctgccgaaa gagtctcgtt
     1021 actggttacg tgaaattttg ttatgtgccg atggtgaacc gtggcttgcc ggtcgtaccg
     1081 tcgttcctgt gtcaacgtta agcgggccgg agctggcgtt acaaaaatta ggtaaaacgc
     1141 cgttaggacg ctatctgttc acatcatcga cattaacccg ggactttatt gagataggcc
     1201 gtgatgccgg actgtggggg cgacgttccc gcctgcgatt aagcggtaaa ccgctgttgc
     1261 taacagaact gtttttaccg gcgtcaccgt tgtactaaga ggaaaaaaat atggagtgga
     1321 gtctgacgca gaataagctg ctggcgtttc atcgcttaat gcgtacggat aagccaattg
     1381 gcgcgttact gctgctctgg ccaacattat gggcgttgtg ggtggcgaca ccgggcgttc
     1441 cccagctctg gatcctggcg gtgtttgtcg cgggcgtctg gctgatgcgc gctgctggtt
     1501 gcgtggtgaa tgattatgct gaccgcaagt ttgatggtca tgttaagcgc acggcgaacc
     1561 gaccacttcc cagcggcgcg gtaacggaga aagaggcgcg cgcgctgttt gtagtgctgg
     1621 tactgatttc atttttactg gtgctgacgc tgaatacgat gactattctg ttgtcgattg
     1681 ccgcgctggc gctggcgtgg gtatacccgt ttatgaagcg gtatacccat ctaccgcaag
     1741 tggtgctggg cgcggcgttt ggctggtcga ttccaatggc ttttgccgct gtgagtgagt
     1801 cggtgccatt gagttgctgg ttaatgttcc tcgccaatat tctctgggcg gtggcttacg
     1861 acacgcagta tgcgatggtt gaccgcgatg atgatgtgaa gattggcatt aaatccaccg
     1921 caatcctgtt cggccaatac gataaattga ttattggtat tttgcagatt ggcgtactag
     1981 cgctgatggc gatcatcggt gagttaaatg gcttaggctg gggatattac tggtcaattc
     2041 tggtagctgg cgcgctgttt gtttatcaac aaaaactgat tgccaaccgc gagcgtgaag
     2101 cctgctttaa agcatttatg aataataact atgttggtct ggtactgttt ttagggctgg
     2161 caatgagtta ctggcatttc tgatgatgta aaaaagccgg atgatcatcc ggctttcttc
     2221 tgggttgcct gatgcgctgc gcttctcagg cctacacaac atatcgcaat ttattgaatt
     2281 tgcagatcat ggtaggccga ataaggcgtt tgcgtcgcat ccggcaattc tcactgatta
     2341 cccttcgccc tgcgtcgcac tctcaatcgt caaacgcacg tctgatgtaa tcaactccgc
     2401 cagcaactga tacaccttca tcgtttctgc cggttcggcg tcgccgctat cgctgatata
     2461 cccttcatca cgcagtgtca gcaccagaga actgaacacc gccttgtcga agaactccgg
     2521 cgcgttgata ccgtgcagca cggagagacg ttgcgcgacg gtgcggctct ctttctccag
     2581 cgtaccgcgg ttgatcgacg ggttggcact caacagccag aaggtgatgg cataacgttg
     2641 cagcgtttcg cgcgcgcctg cggccagcag ctgtagcgtg cgagaatgcg ccgggttgat
     2701 atgcaactca tcatcttgca gggtaatcag cccctgacgt tgcatctcat ttgccagcgc
     2761 atcaataacg tccggtaact cgtcgcgatc ccagcgcagg aacagctccg ctttcagcat
     2821 tgggtaaagc acattgacgt gctccatcaa tacgtcgcgg gagatgtggc gatgctgggt
     2881 gacaattgcc gccatcagcg aaggcagcac caacatatgt gcaatgttgt tgcgatagta
     2941 ggtcatcagc accgcttgct cgcgcggcag aatgatgatg tcgccgatag tgtctttctc
     3001 aacttcaaac ttgttcattt gcagcgcgtg atcgataagc tcgctggcgc tggctgaann
     3061 nnnnnnnnct gacgtgacgc cagaagcgca gtacagcaca ggttcatggc gtttgccgcg
     3121 cctgcgttgt taatgcgtac catcagatcg gcagcaatat tattgaccgt cggcgttaac
     3181 caggccggac gcaccgcttc gatgggatcg atagattcac gccagtctgg tacgtgctgg
     3241 ttaaggtagg tcatcaacgg cattggttca ccgaagttga cgtacccctg accgagatta
     3301 cgcagcttgc ttaaaccacg cagcatctgc ggcaggctct ctttctcttt cgtcgcaccg
     3361 cgcagttctt tggcgtaagt acccacttcc atgacgtgct cataaccgat atagatcgga
     3421 atcagcgtaa tcggacgagt gccgccacgc agcatcgcct gaatggtcat cgacagcgta
     3481 ccggttttcg gatccagcaa acgccccgta cgggaacgac cgccttccac gaaatactcg
     3541 acggaataac cacggctgaa cagttcgccg agatactcac ggaaaacggt ggaataaagt
     3601 ttattgcctt taaacgtacg gcgaataaag aacgccccca gacggcggaa aatcggcccg
     3661 gccggccaga agttcaggtt gatcccggcg gcgatatgcg gcggcaccag cccctggtga
     3721 tacagcacgt aagaaagcag caggtagtcc atgtgactgc ggtggcaagg cacatacact
     3781 agctcatggc cgtcgtgggc cagctggcga acgcgctcgg cgttatggac gttgataccc
     3841 tggtaaagtc ggttccaggt gaagcccaga atgcggtcag tcaggcggat catctcgtaa
     3901 gagaaattcg ccgcaatctc ttccatcagc gcaatcgcgt tctgctgcgc tttttcatgg
     3961 gagatttttt tgctgcgtgc ttcatcttct accgctttcg caatggcgcg ggaggcgagc
     4021 agcttattaa acagatcctg acgagcaggc agacgtggac ccacggcagc cagacgttga
     4081 cgggcaaagt gcatacgcgc cacgcgcgcc agtttctgag cgatagtttt atccgtgccg
     4141 tgttcatccg ccatacggcg cagcgaaact gacggcgaga aacgcacaaa actgtcgcga
     4201 ccgagccaca gtacagcgaa aaatttctgt acgccgttaa gcatacgcag cggcgggttc
     4261 acttcgcctt tttcacgccc cggcgcgcga ccaaacatca ccgatactgg caccatctgc
     4321 acatccagat ttgggttgct gcggtgcaaa tcgagatagt cgtggaacag cttaatagac
     4381 tcttctttcg gcgtgtaata ggtgaacaca cgcggcccgc cgtgaatgaa cacatagcgc
     4441 ggcagtagcg tgccgtcgat ttccagcggc tctaacgggt caggcaggtc atgtgccaga
     4501 cactgggcgc gcaacgtcag caagtcagct ttcgagttgt acggcaaaac gtacataatt
     4561 ggacgagagg tatccagccc cagttccggg gcaggatccg ccggaataga cttgcttttt
     4621 accaggatgc ttaatggtaa attcagtaat ttgtagtaaa ttcgtggcca gccggacata
     4681 aacgatgtaa agcctctggt taataatgca aatgcgcggc aaggatagca gaaagtcatg
     4741 ggaaattctg tggtatccgc tcatgtttcg cgcggcgtta cgcaaacccg aatcatcgga
     4801 tttaacggta cactgatatt gacgctcata atgtaaaaag gttctttcaa tggccaataa
     4861 taccactgga ttcacccgaa ttatcaaagc tgctggctat tcctggaaag gtttacgcgc
     4921 tgcatggatc aacgaagcgg cattccgtca ggaaggtgta gcggtattgt tagcggtggt
     4981 tatcgcctgc tggctggatg tggacgcgat tacccgcgtg ctgcttatca gctccgtgat
     5041 gctggtgatg attgtggaaa ttctcaatag cgccatcgaa gctgtggttg accgaattgg
     5101 ctctgaatac catgagcttt ccggacgcgc taaagatatg ggatccgccg cagtgctgat
     5161 tgccattatc gtcgccgtga ttacctggtg catcctgtta tggtcgcatt ttggataacc
     5221 cttccagatt tcgataaatc tctggtttat tgtgcagttt atggttccaa aatcgccttt
     5281 tgctgtatat actcacagca taactgtata tacacccagg gggcggaatg aaagcgttaa
     5341 cggccaggca acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc
     5401 cgccgacgcg tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag
     5461 aacatctgaa ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg
     5521 ggattcgttt attgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg
     5581 gtgaaccgct tctggcgcaa cagcatatcg aaggtcatta tcaggtcgat ccttccttgt
     5641 tcaagccgaa tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca
     5701 ttatggatgg cgatttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtct
     5761 ttgtcgcacg tattgatgac gaagttaccg ttaaacgcct gaaaaaacag ggcaataaag
     5821 tcgaactgtt accagaaaac agcgagttta aaccaattgt cgttgacctt cgtcagcaga
     5881 gcttcaccat tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctgtaacata
     5941 tctctgagac cgcgatgccg cctggcgtcg cggtttgttt ttcatctctc ttcatcaggc
     6001 ttgtctgcat ggcattcctc acttcatctg ataaagcact ctggcatctc gccttaccca
     6061 tgattttctc caatatcacc gttccgttgc tgggactggt cgatacggcg gtaattggtc
     6121 atcttgatag tccggtttat ttgggcggcg tggcggttgg tgcaacggcg accagctttc
     6181 tctttatgtt gttgctgttt ttacgcatga gcaccaccgg gctgactgcg caggcttatg
     6241 gtgccaaaaa tcctcaggca ttagcccgtg cg
//
LOCUS       DAWDRC010000183         6233 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5784_length_6233_cov_2.373907, whole genome shotgun sequence.
ACCESSION   DAWDRC010000183 DAWDRC010000000
VERSION     DAWDRC010000183.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5784_length_6233_cov_2.373907"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1422..1431
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2044..2053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3216..3225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5547..5556
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1466 a         1638 c         1610 g         1479 t
ORIGIN      
        1 tctgctcggt gcataaactg cgcccggatg tgctgtttct cgatatccag atgccgcgca
       61 tcagtggtct ggaaatggtg gggatgctcg acccggaaca tcgcccgtat attgtttttc
      121 tcaccgcgtt tgacgaatac gccattaaag cctttgaaga acatgccttt gattatctgc
      181 tgaagccaat tgatgaagtg cgactggaga aaacgctggc gcgattgcgt caggagcgca
      241 gcaagcagga tgtttcgctg ttaccggaaa atcaacaggc gctgaaattt atcccttgta
      301 cggggcatag tcggatttat ttgctgcaaa tgaaagatgt ggcatttgtc agcagtcgga
      361 tgagcggtgt ctacgttacc agtcacgaag ggaaagaggg ctttaccgaa ctgacattac
      421 gtaccctgga aagtcgtaca ccattactgc gctgccatcg tcagtatctg gttaacctcg
      481 cgcatttaca ggagattcgt ctggaagata acggccaggc cgagttgatt ttgcgtaatg
      541 gcttaaccgt gccggtcagc cgccgttatc tgaaaagctt aaaagaggcg attggcctgt
      601 aaaagactgc taaaatggct ttttgcctca tcaacacctg aaggcctcat gctaagtaac
      661 gatattctgc gcagcgtgcg ctacattttg aaagccaata ataatgacct ggtgcgtatt
      721 ctggcgctgg gtaatgtcga agccaccgcg gaacagatcg ccgtctggct acgtaaagaa
      781 gacgaagagg gttttcagcg ttgtccggac attgttttgt cttcattcct caatggcctg
      841 atttatgaaa aacgcggcaa ggatgagtct gctccggcac tggagccgga acgtcgcatt
      901 aataacaaca tcgtgctgaa aaaattacgc atcgcgtttt cgcttaaaac cgatgacatt
      961 ctggcaatcc tcaccgaaca gcagttccgc gtttcgatgc cggaaattac ggcgatgatg
     1021 cgtgcaccgg atcataaaaa cttccgcgaa tgcggcgatc aatttttacg ttattttctg
     1081 cgtggactgg cagcgcgcca gcatgtgaag aaaagctaag acgggtatgg cggccatgcg
     1141 aaacatggcc gccgacagat tatttcactt ctttaaaacc agcggctttc atcaccagtt
     1201 ccatttgcgc catagtgata ccttttttgg catcttcagc agaaacgttg attcctgaaa
     1261 taccctgcag ggctttaaaa tccacttttt ccatatcaat agtcacgttt tcctgcgcgt
     1321 aggtatcggt ataggttaat ttttcttcaa cacccgcgat gtttttgtat ttggcgctta
     1381 acggctcaag tgtcctggcg gcatcttctt tggtggttgc annnnnnnnn ncataggtaa
     1441 tggcaatttc agtgccgttc agattggcgc tgaatttctt tgattcttct ttgtcaccgc
     1501 agccagcaag agagaaaacc agaacagatg caacaacaag ggaaaacagc ttattgaaag
     1561 ccttcatgta aaactccatt ttatttaatc aaggaactgg tgactctcac caggggctat
     1621 ataggatatg cctaatactg tggcgtgagc agtccggaac aggagtagaa ctcttaataa
     1681 aaagcactat ttcatccttg ttgctgaagc atggggaata attgttcgca aagcaaaaca
     1741 ccgttattca ttgcttctac ccgtgcctcg ctttctgtat tacgaaattg tcccaacaca
     1801 tgtgccagcc gataaaaacc caccgcggtg aggtcattcg ccagcaactc tgcctgacca
     1861 atagcactct gttcctgata gcgccagccg ttatggagca gttgaataag taacgcctgg
     1921 cagcgcatca gcaactgatg agcggtagac ggcacaggca gaacgctggc agaaggtagc
     1981 ggtgccacag gcgcagtttc tgcgtccagc gcccaggcgc gggtttttgt catcatcacc
     2041 cgtnnnnnnn nnnggttcca gtgtcaattg cccctctaca aaactgacaa agccagaaac
     2101 caggctcacg gggtcgtctg tttgttgcaa aagcgccgcc atgcgttcaa cggcaaaagg
     2161 agaacaggca gatgccggaa gggataacgt cagcacatta tcttcccctt cgccgctgat
     2221 tacctgcgca tccagtgtct ggcggctgct atcccaaccg agcgaaatac actccgctat
     2281 cggcagaata aataagttat cgacctggtt gattggtcgt atgcaggcgg ggggacgctg
     2341 gcgtaaatat tcccgcaaag ccacaatgcc cggctggcgc aacggcgcgc tcaacattag
     2401 ccaggcgtct ggcgacagtg gaacaacgcc actcaagcgg ttacgggtcg cgaacagcag
     2461 ctcgccatcg gcactgcgtt ttgcagcttg tgaaacaatt tgcccacctg ccagtgcgcc
     2521 agcctgaaaa ctaaacagcc gacgcgtcgc tgccggtgag ttttcctgtt cgcttctctg
     2581 ccaactacgc gaaagatgca aaatacttcc tgtgtcggga tcggtaaacc agatgcgtaa
     2641 accatactgc tcaatatcct gccagcaacg catacctaaa gacaccagcc gcagatgatc
     2701 aagttttgct tctccggcaa taccagcgcc aacgaccgtg cgccacggca tgggaggaac
     2761 ttcaccgata ctgtcgcgcc gcgccatctc ttgtacgcaa tttaatcgac tgtttaatgc
     2821 cgcaagctga cgtaagcatt ctccggcatg gtagtggctg gcacgggcgt ggaaggcatc
     2881 aacactggcg cgcaattgcc gtagcgattc actcacccat cgccagttgc agcgttccgc
     2941 cgcttgctgc gcgcgactga aagcggcctc gtaatggatt ggcggttgac ttatcccgcc
     3001 cagccataat gcctggctta attgctgaac atattgacga cacgttttgc cttcttcgct
     3061 ggcaaacgga tcatcagatg atgtgacgtg ttcactgcgc atctgccaga ttaaatgagt
     3121 aaattccgct tgctgagttt tggcctcgac gaaggcttgc acagccagta cgacatgttc
     3181 gcaaagtgtg ccttcaatgc aatcacagcg tgcgannnnn nnnnncgggc aggttagcca
     3241 gttctttcag ccagattgct ggatcccact cttcttcttt tttttccgta ggctgggcgg
     3301 tggcacaaag tcgttgataa cttaacacca gcatcacgcg atgacggcac atgccgctgg
     3361 ccccgcaggt gcactgagcc tctttcagtg cctggccgtt cgccagctgg gtacggacac
     3421 cgtcactgaa ggtggcgatt aaagcgccgt tctcatgact gatttccggg acgttgccat
     3481 tttccagttc cttaaggctg cgcttaacaa aaccggcatt gcttaacgcc gtcagtgcct
     3541 gtggtgtcag ttctaataat tccggacgta gtgaattcat gactgaagat tctccgcaag
     3601 ccaggaagcc agctcgcccg gtgtcattgc ggctatttgt gcgccgacat taaccagcgc
     3661 ctgggccgta tcgtggtcat agcaaggcgt tgcgttgctg tcgagcgctg ccagccccag
     3721 cactttgatg ccgctctgga cacacttttt cacctgatgc gtcagcaatg atgatgaccc
     3781 accttcataa aaatcgctca cgagaataat gacacttttt gcaggttgtt caataagttg
     3841 ccgaccatac tccacggcac tggcgatatt ggtcccgccg cccagttgta ctttcattaa
     3901 taactctacc ggatcggcaa cgtctgccgt gagatcaacc acgctggtgt caaaagcgac
     3961 cagatgggta cgaatgccgg gtaactgcca taaacaggcc gccatcactg cggagtggat
     4021 caccgaatca accatcgatc cgctttgatc aaccagtagg accagttgcc attgttcgct
     4081 atggcgttta atgcggctgt taaagcgggg ggattcgata tacaacttgc cgtgttgcgg
     4141 gtgccagtgt tgcaggttgg cgcgcagagt acttttgaaa tcaaagtttc gcgccagtga
     4201 aataaatgag cggcgacggc gatcgcgtac tccagaaaaa gcctgacgaa cttcctttgc
     4261 cagtcgcgcc ataatctctt caacaacctg gtgcactatc cggcgggcgg cagccagtac
     4321 ttcgggattc atcagatgtt tggtgtgcaa aacggcgcgt agcaggcttt cagaaggctg
     4381 catacgttcc agcacgtcga gattcgtcac cacatcttca atgccgtagc gcagcacggc
     4441 atcgctttcc agccgctcaa tcacctgttg cggaaacagc gtgtgaatgc tgttgatcca
     4501 ctcaggggtg gtgagatttg aacgacctaa tccaccgtaa cgttcaccac gctggagccg
     4561 ttcaggatcg cgcccataca gccactccag cgcgtggtct atctgccggg cgttgtcatc
     4621 cagcccacaa agcgtcgttt ctgccgcttc gccaagaatt aatcgccagc gttgtagctc
     4681 acgggtggtc agaagatcgt tcagttcaga catggaaaac tccccagtgt tgcagcgatg
     4741 ccagtgcctg ctgttcgagt tgttgatgat gtgcaatgtc ttgtggtgga caatgcaacg
     4801 gcatttgcag tgccgaaacg ggaagttgca ccagttgata atgctcaagc acctgatgcg
     4861 ccagcgtccc gcgttctcga ggtggtagcc aggccatcgc cgcgcgtaaa tcgggcaggg
     4921 cattgataaa atcggcatca ctcagttgat ttaaatgact gctgaaaccg gcgataaata
     4981 ccggctgaca ggccagttga tgacgggcta atgccagcaa accgtgcagc gcctcgccgg
     5041 attgtgccgg ggataactgc gccagcatcg tcagcgccgc ttcggcactg gcgttgggat
     5101 gttccagacg catcagtgcg ccaagagtcg cgccgcgatc cagcgccggg gcatgaatcg
     5161 cctgactgcg gcgctccagt aaggctaccg ccgcagaaag agaaacgccg ggtaaattaa
     5221 cgccgctatg tagatcgcgc aggatatggc aaagcgcctg ccagctatgc aggtgagtgt
     5281 gaaactcctt ttcatccggt ctgccgttgg attcacatag ccacagcgtg cgatcgatgg
     5341 ccgcgcataa tgtcatttgt aatatctgcg cgccttgcat accgctaatt tcatccaacc
     5401 gccataaggc atatagcact tccagtgcct ggcccatttc ggcgaattga ttttcctggg
     5461 cgattaataa tgtgagttgt tccagtaatt gttgactgaa agacgccaga cccgctaacg
     5521 ctgcctggct aagacatgtg gtgatannnn nnnnnnatac agccgattcc acccgcgtcc
     5581 agcatatcgg cttctaattt atggcgtgcg gcttcctgga gtgtggcacc aaagcaggcg
     5641 gcctcaatca atgcagcatg ctggctaagc gggcgggtta atttccagca ttcttcaccg
     5701 ttgcctgcaa gtgacagtgt acttccctgc tggcgcacaa cccctgggat ctccagtatt
     5761 gccaaccgat gtaacacctg actttgcgcc agtccatcgg gggtaaagcg atttaaggta
     5821 agctccgccg gaagagagat tccggtacgt tccagttcgc aggtgacatc ttttggcaga
     5881 ggcggttgtg gcgtagaagg ggcaagttta ccgaatccgt cacccgctaa tgtgtctatc
     5941 aacgttacca gaatcggatc ggtatcggga tgaataactc cgcggtagct ccacggcaac
     6001 ggcgcgttca aggcttcttt aatcagcgag ccagctatgg catccagcca gtcagtgcgt
     6061 agaggtagtg tatggccgcg caactgtgcc agtgccatag catgcagatg agcagcagcc
     6121 atatcagcgg tagaagcggg caatttgtgc tggcgcaaac gggtaagaac cgttttcagc
     6181 agttgttcac ctgcctgctg taagccacac tgccagcacc agttttgcca gac
//
LOCUS       DAWDRC010000184         6222 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5801_length_6222_cov_2.333874, whole genome shotgun sequence.
ACCESSION   DAWDRC010000184 DAWDRC010000000
VERSION     DAWDRC010000184.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5801_length_6222_cov_2.333874"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    694..703
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1586 a         1588 c         1502 g         1536 t
ORIGIN      
        1 aaaggttacg atcacgcgtt cttgttacag gccaaaggcg atggcaagaa agtggcggcg
       61 catgtctggt cagcagatga aaaattgcag ctgaaggtct acaccaccgc tccggctctg
      121 caattctact ccggcaactt cctcggcggc acaccgtcgc ggggaaccga accttacgcc
      181 gactggcaag ggctggctct ggaaagcgaa tttctgccgg acagcccgaa ccaccctgaa
      241 tggccgcaac cggactgctt cctgcgtcct ggcgaagagt attccagcct gacggaatat
      301 cagtttattg ctcagtaatg ttaagccctc gtcatgaggg ctttatcttc atattgttca
      361 aatcaccagc aaacaccgac atatttgcaa ctcaatattc acaacaacct tacactgcga
      421 cactattttc gctatggtta tgcgtaagca ttgctgttgc ttcgtcacgg caatataatg
      481 agaattatta tcattaaaag atgatttgag gagtaagtat atggctgtaa ctaagctggt
      541 tctggttcgt catggcgaaa gtcagtggaa caaagaaaac cgtttcaccg gttggtacga
      601 cgtggatctg tctgagaaag gcgtaagcga agcaaaagca gcaggtaagc tgctgaaaga
      661 ggaaggttac agctttgact ttgcttacac ctcnnnnnnn nnngtctgaa caaagcggaa
      721 accgctgaaa agtatggcga cgagcaggtg aaacagtggc gtcgtggttt tgcggtgact
      781 ccgccggaac tgactaaaga tgatgagcgt tatccggggc acgatccgcg ttacgcgaaa
      841 ctgagcgaga aagagctgcc gctgacggaa agcctggcgc tgaccattga ccgcgtgatc
      901 ccttactgga atgaaaccat tctgccacgt atgaagagcg gtgagcgcgt catcatcgcc
      961 gcacacggta actcgttacg tgcgctggtg aaatatctcg ataacatgag cgaagaagag
     1021 attcttgagc tgaatatccc gaccggcgtg ccgctggttt atgagtttga cgagaacttc
     1081 aaaccgctga aacgctatta tctgggtaat gctgacgaga tcgcggcgaa agcagcggcg
     1141 gttgctaacc agggtaaagc gaagtaaacg tcattcgttt aaaaatgaga aagccgaccg
     1201 caaaatgagt cggctttttt atttttcgtt gctaccaatg gagcacatcg cctgatgcga
     1261 cgctgcgcgt tttatcaggc ctccggtgat taatcggata cgccactctg acggcgcatc
     1321 cgacaattaa actttacccg cgacgcgctt ttactgcatt cgccagttga cgtaacagag
     1381 catcggtatc atcccagcca atgcaggcgt cggtgatgct cttaccgtag gccagcggtt
     1441 ccccgctctc gaggctctga ttgccttcca ccagatggct ttccaccatc acgccaataa
     1501 tggccttttc gccaccggca atctgctggc aaacgtcagt acaaacatcc atctgctttt
     1561 tgaattgttt tgacgagtta gcatggctga aatcgatcat cacctgtgct ggcaggcctg
     1621 ctttgttcag cccttctttc acttcagcaa cgtgcttcgc gctgtagtta ggctctttac
     1681 caccgcgcag aatgatatgg caatcgccgt taccgctggt attcacaatc gccgaatgac
     1741 cccatttggt cactgaaagg aagcagtgcg gcgcaccggc ggcattaatg gcatcgatag
     1801 ccactttaat cgtaccatca gtgccatttt tgaaacctac cggacaagaa agaccagacg
     1861 ccagttcgcg gtgcacctgc gattcggtgg tacgtgcgcc aattgcgccc cagctcatca
     1921 ggtcagcgag atattgtggg gtgatcatat cgagaaactc acccgccgct ggcagaccgc
     1981 tgtcgttaat atcaagcaac aatttacggg caatacgcag accgtcgttg atctggaagc
     2041 tgttatccat atgcggatcg ttaatcagcc ctttccagcc caccgtagta cgcggctttt
     2101 caaaatagac gcgcatcacg atttccagct catcttgcag ctcttcacgc agcgtcagca
     2161 agcgagcggc atactcttta gccgcgacag gatcatgaat tgagcatggg ccaatcacca
     2221 ccaacaggcg atcatcatta cctttcagga ttttatggat cgcttttcgg gcatgggcga
     2281 ccgtattcgc ggcattttca gtagcgggga atttttccag caatgcgaca ggaggaagta
     2341 actctttgat ttctttgatg cgtaaatcgt cgttctgata attcatgtct gttccagtgt
     2401 tgccatactt atcttagtga atgcaatact tccaatctat atcttccgtc agaatgtgta
     2461 aacggggttt tacactatga acggattaat cctggaatta gcaagaaaaa cgccagattg
     2521 tcgcgaaaaa cgagatctct cctacaattt ctaactgtaa ctcctttgtt tgttaattat
     2581 ttcaagattc tctgctgggt atctttgcct ggctgaaata accattgcct tatattcacc
     2641 tgcaaatgca ctgttggaag aggttatccg acataacgac cataacagga gcatcctatg
     2701 aaaatgacaa aactggccac actatttttg actgccactc taagccttgc cagcggtgcc
     2761 gcactggctg ccgatagcgg agcgcaaagc aataacggcc aggcaaacgc cgcagctgat
     2821 gcgggccagg tagcccctga tgcccgtgaa aatgtcgcgc caaataacgt cgacaataac
     2881 ggggtaaata ccggttctgg cggcacaatg ctgcatccgg atggttcttc aatgaacaat
     2941 gacggcatga ccaaagatga agagcacaag aacaccatgt gcaaagatgg tcgctgcccg
     3001 gatatcaata aaaaagtcca aaccggtgat ggcatcaaca atgatgtcga taccaaaacc
     3061 gacggtacca cgcagtaacc ctgcgaaata gccaataatg ggagagcgga gtctctccct
     3121 tttaattttt cccttctaaa agcagtatga tgtcataaag ttaagtgaga ataattaagg
     3181 gatgactcgt catggcgcac tcacactcac acacttcctc acacctgcca gaagataata
     3241 atgctcgtcg cttgttgtat gctttcggcg tgactgccgg gtttatgctc gtagaagtca
     3301 ttggtggttt tctttccggt tctctggcat tgctggccga tgcgggtcat atgttgaccg
     3361 atactgccgc cctgcttttc gccctactcg cagtgcaatt ttcccgtcgt cctcccacca
     3421 ttcgccacac tttcggttgg ctaagattga ccacgctcgc ggcgtttgtg aatgccatcg
     3481 ccctggtggt gattactatt ttgattgtct gggaggcgat agaacgcttc cgcactccgc
     3541 gtccggtcga gggcggcatg atgatggcaa ttgccgtggc tgggctgctg gcaaatatac
     3601 tttctttctg gttacttcat cacggcagcg aagagaaaaa cctcaacgtg cgagcagcgg
     3661 cactgcatgt gctcggggac ctgctgggtt cggttggggc tattattgcc gcgttgatta
     3721 ttatctggac cggctggaca cctgctgacc ccattctctc aatactggtg tcgcttctgg
     3781 ttctgcgtag cgcgtggcga ctgttgaaag atagcgtgaa tgaattactt gaaggtgcac
     3841 cggtatcgct ggatatcgcc gaactgaagc gtcgtatgtg ccgggaaatc ccggaagtcc
     3901 gcaatgtgca ccatgtacat gtatggatgg taggcgagaa gccggtgatg acgctgcatg
     3961 tgcaggtgat cccgccacac gatcacgatg ccttgttaga tcagatccaa cactacctga
     4021 tggatcacta tcagattgag cacgccacta ttcagatgga atatcaacct tgtcatgggc
     4081 cagactgcca cctgaatgaa ggggtgtctg gccattcaca tcatcaccat taatgggaca
     4141 gcgcgcgtga acctctttcg cgtgcgctgt tgatccacat ccggctgccg ttgagggcaa
     4201 taaaggtcag gatgatgtac tccagagaca tggcgtaaac gccctgaagt gcaaaaataa
     4261 cgacgctaat cacgttaata atcacccaca acagccagtt ttccacatac ttacgcgtca
     4321 tcagaatcat tgccacgata gataacacca tcatgcatga atcccagaac gggaaagcgt
     4381 ccggctgtag ttcaggcatc gcaacctgta atcctaatgc ttgcatgatc atgactgcca
     4441 cgcgggtcaa aaatgcaaac accggattga taaataccgt catcagacca atcgaaacaa
     4501 cgcaaaccgc caaccagctt agcgccttcg gcaatggcaa ccagcgaatt ttcaactccg
     4561 cctcgttctg actggtttgt cgcgaccacg cataccagcc gtaaatattc gcggcaaaga
     4621 aaaacacctg taatagcagg ctggcataca gctgaatctg aaagaaaata atgccaaaca
     4681 aggtgacgtt gatcaggcca aagaagtagt tactgatctt ctccagactg gcaaggccaa
     4741 tacacagcaa cccggcgatc gtgcctaccg cttcgatcca tgagagatca taaccgcctg
     4801 cccctattgg tatatgtacc aggatattct gcacactaaa aaaatccatc gttcccccaa
     4861 agcgtaatgt gacgccttat tatcaattat ccacgtagtg tagccgcaaa atccagcata
     4921 cgattgagcg gcaccagcgc cctctctagc agcctttcat caacataaac ctcgtgattg
     4981 cttccttcca gttctaatgc ctctgcgatg gcctgaaggc cattcatggc catccacggg
     5041 caatgcgcgc agctgcgaca ggttgcgccc tcacccgcgg ttggtgcttc cagtaactct
     5101 ttatctggca ccgcctgctg cattttgtag aaaatgcccc gatcggttgc cacgataagc
     5161 cgctgatgtg gcaatgtttt cgcagcagcg atcagttgac tggtggaacc gaccgcatcc
     5221 gccatctcta caatagcttg cggtgattct ggatgcacca gtatggcagc atccgggtat
     5281 tcttcttgca agcgggttaa cgcctgagtc ttaaactcat catgcacaat gcaggcaccc
     5341 tgccagcata aaatgtctgc gcccgtctgt ttttgcacgt aacaccccag atgtttgtca
     5401 ggtgcccaga tgattttttc acccaaacta tccagatgat caataagttc gacggcaatg
     5461 cttgaagtta ccacccaatc tgcgcgcgct tttaccgcag cagaagtgtt ggcgtagacg
     5521 acgacagtac gatcgggatg ggcatcgcaa aatgcgttaa attcttcaac agggcagccg
     5581 agatccagtg aacattcagc ctgaagtgtc ggcatcagaa ttgttttttc cggactgaga
     5641 attttggcgg tttctcccat aaatctcacc ccagcaacta acaacgtaga agcgggatgc
     5701 tttgcaccga agcgcgccat ttccagagaa tcagaaatac agccaccggt ttcttctgcc
     5761 agttgttgaa tttcgggatc ggtatagtag tgggcaacca tcaccgcatt acgttctttt
     5821 agtagacgtt ttatcttctc gcggtaatac gctttttcat caatgcttaa cggcgtcggc
     5881 ttcgggggga aaggataaat cgccgtgtct ggatcaaaca tcacgctcat cttaccatct
     5941 cgttttacag gcttaacgtt aaaaccgact taagcgtgat attcgctatt atcggcgtaa
     6001 tttgttttat atgctaaaca agatagtcga atggtgggga aaagtcactc gaattacgta
     6061 tgagatgccg aatgccacac tttgcgtctt attcctccga cgtagagttg tttacgcagc
     6121 aagcgcggag tatgtcatca gggtcaagaa ttgaaattga gagatgcctg aatagcaaaa
     6181 aaacgccttt agggcgcttt tttgcattgg tgggtcgtgc ag
//
LOCUS       DAWDRC010000185         6212 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5816_length_6212_cov_2.216989, whole genome shotgun sequence.
ACCESSION   DAWDRC010000185 DAWDRC010000000
VERSION     DAWDRC010000185.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6212)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6212)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5816_length_6212_cov_2.216989"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    508..517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1546 a         1483 c         1463 g         1710 t
ORIGIN      
        1 atatttcttg cgacgttcgt gctgtctgca gacgaaagcg gtacatgagt aaaaatgtct
       61 ttatttaaca tccgtaaccc atgaatcttg gtgattgggt agccgtactg atctacaaca
      121 tgacgtataa gatcgcgtag tttagcccga cacgctctcg gtcgttttgc atcgtattcc
      181 cccatcgagc cgatgcagac gcggggaaac tcatggcaca gacgaataaa tcgctcatcc
      241 ggttcgttca tgtgccacac cggagcacca ataaatttac cgtgaggcca tgccgcaatc
      301 agggcgtcat tctcttcact ggttctgccg ataacatccg ggataaccgc gaatgagaaa
      361 cgagggtgat taccccagcg ttcaacaaat ctgtaatatt cattccagtc tatggccttg
      421 ttttttgtcc agaatgtgaa tgcaccatta tcaagagcaa atgattgggt gacttcggaa
      481 gccagatcaa tctgagctgg attagcannn nnnnnnnaat gcgtgtctgc ctttccaggc
      541 tttcaacgca caggtatcgg gagttattgg accaccgtga aaatgaatca tacactctcc
      601 cgtttattat ttatctcctc agccagccgc tgggctttca gcggatttcg gataacagaa
      661 aggccgggaa atacccagcc tcgctttgta acggagtaga cgaaagtgat cgcgcctacc
      721 cggatattat cgtgaggatg cttcattgcc attgctcccc atatacaaaa ccaatttcag
      781 ccagtgcctc gtccattttt tcgatgaact ccggcaccat ctcgtcaaaa ctcgccatgt
      841 acttttcatt ccgctcaacc acgacataat gcaggccttc acgcttcatg cgcgggtcat
      901 agttggcaaa gtaccaggca tcttttcgcg tcacccacat gctgtactgc acctgggcca
      961 tgtaagccga ttttattgcc tcgaaaccac cgagccggaa tttcatgaaa tcccgggagg
     1021 taaacgggca tttcagttca aggccgttgc cgtcactgca taaaccatcg ggagagcagg
     1081 cggtacgcat actttcgtcg cgatagatga tcggtgattc agtaacattc acgccggaag
     1141 tgaactcaaa cagggctctg gcgtcgttct cgtactgttt tccccaggcc agcgctttag
     1201 cgttaacttc cggagccaca ccggtgcaaa cctcagcaag cagggtgtgg aagtaggaca
     1261 ttttcatgtc aggccacttt tttccggagc ggggttttgc tattacgtta tgaacttctg
     1321 aagctgtgat gacgccgagc cgtaatttgt gccacgcatc atccccctgt tcgacagctc
     1381 tcacgtcgat cccggtacgc tgcaggataa tgtccggtgt catgctgcca ccttctgctc
     1441 tgcggctttc tgtttcagga atccaagagc ttttactgct tcggcctgtg tcagttctga
     1501 cgatgcgcga atgtcgcggc gaaatatctg ggaacagagc ggcaataagt cgtcatccca
     1561 tgttttatcc agggcgatca gcagagtgtt aatctcctgc atgctttcat cgttaaccgg
     1621 agtgatgtcg cgttccggct ggcgttctgc agtgtatgcg gtattttcga caatgcgctc
     1681 ggcttcatcc ttgtcataga taccagcaaa tccgaaggcc aggcgggcac actgaatcat
     1741 ggttttatgc cgtaacattc gtttgggatg cgactgccac ggccccgtga tttctctgcc
     1801 ttcgcgagtt ttgaatggtt cgcggcggca ttcatccatc cattcggtaa cgcagatcgg
     1861 atgattacgg tccttgcggt aaatccggca tgtacaggat tcattgtcct gttcaaagtc
     1921 catgccgtca aactgctggt tttcgttgat gatgcgggac cagccatcaa cgcccaccac
     1981 cggaacgatg ccgttctgct tatcagggaa ggcgtaaatt tctttcgtcc acggattaag
     2041 gccgtattgg ttggcgacga tcagcaatgc gatgaactgc gcatcgctgg catcgccttt
     2101 aaatgctgtc tggcgaagag tggtgatcag ttcctgtagg tcgacagaat ccatgccgac
     2161 acgttcagcc agcttccctg ccagcgttgc gagtgctgta ctcatccgtt ttatacctct
     2221 gaatcaatat caacctgatg gtgagcaatg gtttcaacca tgtaccggat gtgttctgcc
     2281 atgcgctcct gaaactcaac atcgtcatca aacgcacggg taatggcttt tttgctggcc
     2341 ccgtggcgtt gcaaatgatc gatgcatagc gattcaaaca ggtgctgggg caggcctttt
     2401 tccatgtcgt ctgccagttc tgcctctttc tcttcacggg cgatctgctg gtagtgacgc
     2461 gcccagcttt gagcctcaag acgatcctga atgtaataag cgttcatggc tgaactcctg
     2521 aaaatggctg tgaaaatatc gcccgctaaa tgccaggctg attaggtaaa caggaaaggg
     2581 gattagtgat tcaggccgtt gccgcgtccg tcgagaaaaa cttccacgag caaatcacgg
     2641 gtataagtgc gctcgatgcc gcgatgcaga taaagccgtc cgcgtaaatt agctgatgca
     2701 gtccaggtac catctttgtg tttgaccagc attcctggca tgaccgcacc gcgattaacg
     2761 gtctgcgttc cgtaatgttg atgaaccata aaaactcctg cccgtaagct gggctgctga
     2821 acaaataagg ggttgcgcag tatctggcgg tggatggccg ccggttgtca taactaagtc
     2881 gcctcgttga agcaactgag gtatgaagtg ttgagttgat ttcagctggt cacaccgacg
     2941 ttcacgcgtc cgtttcaccc ctcgcactcc ccggagcctg ctgaaattca agctgcggat
     3001 ctaagcggtc atcgcaacgg tgaatcaggt ggttgccgta tcgttgtgtt gttgcgacat
     3061 ggtgataata gctattgcta ttggtgatat caatacttat tgctattgtt agatgtgttt
     3121 tgctattaaa tgtttgatag caaaaagaat taattttgta acttgaatcg catagcgata
     3181 actgaagcga ggtcgtggtg gttttttgaa cggtttgtgt gatgagggga ggcaaaagaa
     3241 aacccggcac ggtggccggg tatctttatt cgaatggaag atctatttgc ctttgaggat
     3301 tcaactgttt ttttacatgc tcaatttcga atatggtttt tatggcatct ccgatcatat
     3361 attgagttac ttttaaatct acaagtaaca tatcaccttt agaaaaattt aaggtgttgt
     3421 tatcaatatc gctgatgaat ttctggtctt ttatttcagc caaaaagcta ctggcaccat
     3481 cagaaaaccg ccaccggctt ccttcattga aggaaatatt tttaatttga agggctttct
     3541 ctactgtgta ctctgaaatt attgattctg ctggtagatt gactttgaaa taatgtgctt
     3601 cttgcttgtt gatcgtcatg aatgtcaaac catcatcgac agtagaggca aagctgtcaa
     3661 tgccttcctg ctctaatggc ttacttataa cttcttgtaa tgaagagcga agtttaatat
     3721 ttttataaag ttcaataacg ttactgtcgt agatctcgct ttcgtcatca acaaagactt
     3781 cgatgttgct atcaggcaaa ttgtgtaatt tttttatttt ccttggacct atccatttta
     3841 tcagttgaat taggcctttg catatttttt ctttagaacc aggacagagt ccaataaaag
     3901 caatcaaatt cgcagcggct gttgctgaat cgccggataa aaaatcaaca gcctgtttga
     3961 accaagatgt agatgaggca accagatcta ttccaaatga gccagcttta aatgatgcat
     4021 tgacttttac agaaacaaca gtcttgtttc cgtagagagt tttaccggct tcctctaatg
     4081 catctgatag tgatagaagg gcaggagcaa ggtctcgcac gttcatttca tgcgtttcca
     4141 gtgccggacc gtcgtatact atcctgaact tcatatcatt ggtttccgaa ttgttgcccg
     4201 catccattat ggcattcacc tgatcacata tacagttaat ttacgatatt gtcttaaatc
     4261 ttaaaacttc tattgtatgg ctacccatgc ttcctatacg tctgcggcat gctcccaata
     4321 accttaccga agatgaacac ccggttcatc tcgtctttct cgatcgggtc ccaaggtgag
     4381 tagctcttgt tatcagagat aaccagcagc ttatccttca tcatttgcag gcgctttaca
     4441 tgggctgtgt cgtcgtacag aaacgcatag ataccatcac cgtcgaaaga tttaaccgtg
     4501 atatcaacga acagcagatc acctggttcg atcgttcctg acatgctgtc accacgcacg
     4561 ttaatgatgc ggatattttc cgccttcctg ccatcgaaca tgtgacgagc atcgtcaaac
     4621 gagtactcaa ccgagcgtag aacttctaca aactcacgat tgatgacacc cggcccggca
     4681 ctgacttcta tatcaagaac gtcaatcttg aagtatttgg aatggttgac agcaggcttc
     4741 cctgattgtt gaccgtcatt tctcatcggg cctatgcctg atgagagcca ctcagttcga
     4801 acacccaatg cattagctat ttcaacaatt tttgttgagc cgcgcgcgtt gccgcttgtc
     4861 agtctccaga ttgtgggttg agctacgcca gacgcctttg caagagcgcc ttgagacatt
     4921 ccagattgtt ccatcgctag gtttaagcga tcagcaagag tttctttttt cataagtttt
     4981 aatttatacg ctcgcgtatt gatggtcaaa acacgttttg ctattgattg ggttaatacg
     5041 cattgctatt atctattcat tgcaatacca ataggaattg ataatgacaa atcaaaccat
     5101 tcaactcgca atcagtatta caggtagtca aaaacgactg gcagatctat gcggtgtagc
     5161 ccaacccact gtttggcgtt ggctacacgg tggcggaatt gatgcccgct atgtaatgaa
     5221 aattgtctca gccactggtg gaaagattaa accagcagat attcgtcccg acctcgcacc
     5281 attgtttaac gcgagtaatt ctgccgccta aactgcggcg ttaactgata aggcaatgac
     5341 tatgcaatca cttacatacc aacagactag cggatttagc ccgactgcgg tgataaatcg
     5401 ttctcaaaca aaacaggcgc caggccacga aaaaatccgt gatgccgttc gcgcctggtc
     5461 ggctgcagat aatcaggatg ttgttgccgc actcattgtg aatgagtatc gggagcaggg
     5521 cggcggcacc atcgatttcc ctgatgatgt cagccgtgca cgacagaagc tgttccgctt
     5581 cctcgataac aaattcgatt ctgaaaaata ccgaaataac gtgcgtgaac tgaccccggc
     5641 aattctggcg gtactaccgc tggaatatcg cggccacctg gttgggcagg atagcttcat
     5701 ggctcggctg gctgaaatgg aaaaggaact cagtgaggca aaacaggctg tcattctcaa
     5761 cgcaccacgc caccagaaac tgaaggagat gagtgaaggc attgtgtcga tgtttcgtgt
     5821 ggacccggat ctggctggtc cactgatggc gatggtcacc accatgctgg gggcaatatg
     5881 acaggttcag aaatggcgaa agccggtctg cgcgaacaga accgactttc aggtgcaaat
     5941 cgtaacacac tcattgcggg aggaattatg gcaaacactg ctgagatatt caattttcca
     6001 gtgccggatg tggcacaaaa ggagccgcgc gtggcagatc tcgatgatgg ttatacgcgc
     6061 attgcaaatg agttgctgga agctgtaatg ctggccggat taaaacagca ccagcttctg
     6121 gtcttcctgg ctgtcatgcg caaaacatat ggctttaata aaaaactgga ttgggtgagc
     6181 aacgagcaac tttccgaatt gaccgggata tt
//
LOCUS       DAWDRC010000186         6207 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5820_length_6207_cov_2.242360, whole genome shotgun sequence.
ACCESSION   DAWDRC010000186 DAWDRC010000000
VERSION     DAWDRC010000186.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5820_length_6207_cov_2.242360"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3005..3014
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3760..3769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1604 a         1510 c         1639 g         1434 t
ORIGIN      
        1 ccgtggtgtt caacagacgt tgagccgccg ggctacgtcc gcttttaatc accagaatcg
       61 gtttattacg tgaggcgcta cgggccgccg aaacaaaacg tcgcgcatca cttaattgtt
      121 cgagatagag caggatggcg ctggttttac tgtcgcgcgc caaatagtcg agcaattcat
      181 caacgtcgat atccaggctg tcgccgagcg caataaagta ggaaaagccc atctcacgct
      241 gttgcgccca gtcgaggatg gtgttggaga cagcagccga ttgcgaaata aacgccagct
      301 tgccgcgttt aatcggcaca ggcgaaaagc tggcattcag accttgccag ggagccagta
      361 agcccagact gtttggtcct agcaggcgca tgttatggcg cagggcgcag gcgcggagat
      421 cttcgtgttg cgatgccggg gcggaaagaa taatgcaggt tttacagcct ttctcgccga
      481 gctcttccag aagagcaaga ttacggctgg cattggtaca taaaaccgca aggtcgggtg
      541 taaagggcaa gcgggcaata tccggccagg ccaacacacc caacaccgct ttccaggctg
      601 gcgtcaccgg gagtaccggt ccgttaaagc ctcccgccag caggttacgc atcatcaggt
      661 aacctgcgcg attgggtttc atcgatgcgc caattaccgc aatcgatttt ggtcgcagta
      721 gtgcttccag tcctcgctga ctcataccgg tctcccgcag aagtgaccga atgattttaa
      781 acgctttcta actgttctgc tgtgatgctt cccggatgtt gcgtttttcc tgacagatag
      841 cgtgttttaa agcgggtaaa atgctcgcct aaccctgtcg ccgccctggt atcgccggcc
      901 atatctaaca gtgcgatggc tacctcggca gtacaatatt ggccttcagc ctgggcttca
      961 cgcaggcgat aggcagaaag ccgagaaaga tcgacggaaa tcacgggaag attatccaga
     1021 tacggacttt tacgaaacat cttgcgagct tctggccagg taccatcgag catgataaat
     1081 agcggaggct taccggcagg tggtgtgaag atcacttccc gttgctcatc agcatacgaa
     1141 gcgggaaaga ccaccattgg ctgatagtcc gggttttgca ccagatccag caaatcttgc
     1201 gagggttcgg tacgcgacca ttgaaacgca acggtatcag gcaaaatatc agcaatcaga
     1261 cgcccggtat tactgggctt cattggctcg gtgtcgaaca tcagcaaaca gaagcgactt
     1321 tttgcttgtg ctggggtaat tgtcgaacag agacataatt tctctggcaa aagacagcgt
     1381 tggcagcgac gaacgcgatt accgcgggca agaaaaggac gtgttgcgcg cgcaatacgc
     1441 tcggcgcgta actggagaac agcgttttcg gtcataagag agcgtcgaaa aaacgccatt
     1501 gttgcagagg agaaaacggg gcacaagatg cgccccggta agattaaaga gattcgttca
     1561 gccagctgtc gaacggcgct ttcggtactg cgctattaag catgtcaaca acctgaccat
     1621 ttttgaaaat catgatcgtc ggtatactac gaattccaaa gcgactgctc aattcacgtt
     1681 cagcttcggt attcactttc acaaagcgca ctttaccgct acgctcttgc gcaacatctt
     1741 caaaaattgg cgcgaagtta cggcaggggc cgcaccacgg cgcccagaag tcgatcacca
     1801 caggtagatc gtctttcagc aatttgtcga gcgtttcacc ggtcgcatta atcacctctc
     1861 cgtcaaacaa gtcgtgaccg cagcgtccgc attttgccgc atcttcgatc cgatcgtcgg
     1921 gaatgcgatt gatggcctga caatgggtac aaacggtatt cataactaac ctcgggatga
     1981 gtaaggagac ttatcggcaa cgacgccggt atgtttctaa tatgttacat attatcgggg
     2041 acattgttcc agacaacaat ctgttttatt caatgggtat gatagacaca ggctttggaa
     2101 cgaaatcatg gcgaaccgcg cggacttcgg gtaatctgcg cgcttcgcgc agcgctggtg
     2161 gagaaaagca tgaacgatga aatgaaaggt aaaagcggca aggtcaaagt gatgtatgtc
     2221 cgcagtgatg atgattccga taaacgtacc cacaacccgc gtaccgggaa agggggcggg
     2281 cgtccaggaa aatctcgtgc tgacggtggc cgtcgccccg cccgcgatga caaacagagt
     2341 cagcaccgtg accgcaagtg ggaagattcg ccgtggcgca cggtttcccg cgcgccgggt
     2401 gatgagacgc cggaaaaggc cgatcacggt ggcatcagtg gtaaaagttt tattgatccg
     2461 gaagttttgc gtcgtcagcg tgcggaagaa acccgcgtct acggcgaaaa cgcctgtcag
     2521 gcactgttcc agagccgtcc ggaagcgatt gttcgcgcct ggtttatcca gagcgtaacg
     2581 ccacgtttta aagaagcctt gcgctggatg gcggcaaacc gcaaagcgta ccatgtggtg
     2641 gatgaagcgg aactgacaaa agcgtcaggc acggaacatc acggcggcgt ttgcttcctg
     2701 atcaaaaagc gtaacggtac aaccgtgcag cagtgggtaa gccaggcagg cgcgcaggat
     2761 tgtgtactgg ccctggaaaa cgaatctaac ccgcataacc tgggcggcat gatgcgtagc
     2821 tgtgcgcact tcggcgtgaa aggtgttgtg gtgcaggatg cagcactgct ggagtctggg
     2881 gcggctatcc gtaccgcaga aggcggcgca gagcacgttc agccgattac tggcgacaac
     2941 attgttaacg tgctggatga tttccgtcag gcgggttaca ccgtagtgac aacttccagc
     3001 gagcnnnnnn nnnngcacgc gatccgaacg atctgcgcgt gaagattgat ggtactggca
     3061 acgttgccgg gctgaatatc tctgtcgcaa ccggcgttct tcttggcgaa tggtggcgtc
     3121 agaataaagc ctgataaatc agccgggtgg caactctgcc atccggcatt ttcccgcaaa
     3181 tttactcact ctctgccggt aatactggca tccagtcaat cggcgtctcg ccacgttgtt
     3241 ccagccactg atttgccagc acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa
     3301 gcggcgacgg atgcggtgct ttcagtacat gatggcgttg cttatctata atcgcccctt
     3361 tcttttgcgc atgtgatccc cacaacaaaa acaccacgcc ttcgcgatgc tgatttatga
     3421 ggctgatgac tttatcagta aacgtctccc agccgaggct ggcgtgggaa tgcgcctgac
     3481 cagcgcgaac cgtcaagaca gtattgagca gcagaacacc ctgacgcgcc cagctttcaa
     3541 gataaccatg attagggcgg gtgaaaccgg gaatagtatt ttccagctct ttatacatat
     3601 tcagcagtga cggtggtgtc gcaatgccgg ggcgaacgga aaatgccaaa ccatgcgcct
     3661 gtcctggtcc gtgataagga tcctgaccga gaatcaccac tttaacgtca cccaactccg
     3721 taaagcggaa cgcgttaaag acatcttttt gtggtggatn nnnnnnnnng tggtggatag
     3781 atagtgacgc cggactgccg ctcgctggcg acggtctgaa gggtattaag aaaatagggt
     3841 tgctgcttct cttcagccag cacatcatgc caggttaatt cgttagccat ctcgctctcc
     3901 tgcgaatctt taatccgcct agcttaactg cttctttcta cagaacaaaa tcatgctcat
     3961 tttatagaca aaaagacatg caaaaaaatg taaattggta aaatttcaca tctgcataag
     4021 ttgatgtaaa acaataaaat ccatttgtga ccacacttga tatatggttt ttattgattt
     4081 aaatcaaaga atcaagggtg tttgaggagt atatatacac tcaagcaaca atggttttac
     4141 caattggccg cgacaggctg aacaaatcaa ataattttgc cggggaggca tcacatgatt
     4201 acaggtatcc agattactaa agccgctaac gacgatctgc tgaactcttt ctggttgctg
     4261 gacagcgaaa aaggcgaagc gcgttgcatc gttgcgaaag caggttttgc agaagacgaa
     4321 gtggttgcag taagcaaact gggtgacatt gaataccgtg aagttccagt agaagtgaaa
     4381 ccagaagttc gcgttgaagg tggtcaacac ctgaacgtta acgttctgcg tcgtgaaact
     4441 ctggaagatg cagttaagca tccggaaaaa tatccgcagc tgaccatccg tgtatccggt
     4501 tatgcagttc gctttaactc tctgactccg gaacagcagc gcgacgttat cgctcgtacc
     4561 tttactgaaa gcctgtaagg cttaagtagc aagttactaa gcggaagagg gcgacctctt
     4621 ccgctttttt tcgttaatag aaaatgcgta ccgcgtaata gaccagcaac agggcaagca
     4681 cgatatttaa ctggcgacca tactggcgaa acaatcgctg aaacagatgc cccgccagcg
     4741 cccagcacac attgccaaac gtcccaatca tcgccagcaa aacgctgacg ccaactaccc
     4801 agcttagcgc ctgtgtttgc ggcagaacaa acgtcgacag tgccgtaaca ccgtacaaaa
     4861 tgattttgac gttcacaaac tgcaaagcaa agctggccca aaaactgatt ggttttgtct
     4921 gaagtccgtc ttcctttgtt gggctggtgg cgattttcca cgccagccag acaatatatg
     4981 ccgcccccgc ccaactcaaa aggtgtaccg ctgccgggtc aatcactgcc agtgaaaatg
     5041 aaatgcccgc acacagtaac atcacaatca aaaatcccag actcatccct gccagcacac
     5101 gggtactttg acgaaatcca tgcgacgtag cagagctaag ggcgagaata ttgttcggtc
     5161 ctggcgtcat ggcggtaatc agggtgtaag tccaaaaagc acttaaaagg gtcggtgtca
     5221 cttgttatct cctctgtttt ggtagacagt acccgacacg gaacatttaa aaaaatgaat
     5281 gtttttacat tctaaattcg atattttcga taggtttggg gttatggatc tgcgccgttt
     5341 tattacgctt aaaaccgtgg tggaagaggg ttcctttttg cgagcttcgc aaaaattgtg
     5401 ctgtacacaa tcgacggtga cttttcatat tcagcagctt gagcaggagt tctcggtaaa
     5461 attgtttgag aaaattggtc gacgaatgtg ccttacccgc gaaggaaaaa agctgctgcc
     5521 gcacatctat gaacttaccc gagtgatgga tacgcttcgt gaggctgcga aaaaagagtc
     5581 ggatccggac ggggaactac gcgttatttc aggcgaaacg ttgctctctt atcgaatgcc
     5641 gcaggtgttg cagcgttttc ggcaacgcgc tccaaaagtg cgtttatcgt tgcagtcgct
     5701 gaactgttac gtgatccgtg atgcgctact gaatgatgag gccgatgtcg gggtttttta
     5761 tcgcgtaggg aatgatgatg ttctgaaccg acgagagttg ggtgaacaac cgctggtgct
     5821 ggtggcttca ccgcaaattg cagatgtcga ttttactgag ccgggaagac ataacgcctg
     5881 tagctttatt atcaacgaac cacaatgtgt cttccggcag atatttgaga gcacgctgcg
     5941 ccagcggcgg atcacggtgg aaaacaccat tgagcttata agtatcgaaa gcatcaagcg
     6001 ttgtgtcgcg gcaaatatcg gagtcagtta cctgccgcgt tttgcggtgg taaaagagtt
     6061 ggagtgcgga gaattaattg aattgccttt tggcgaacag tcgcagacca ttacggcaat
     6121 gtgcgctcac catgccggaa aagcggttag cccggcgatg cacactttta ttcagtgtgt
     6181 tgaagagtgt tttttgccgg gataaaa
//
LOCUS       DAWDRC010000187         6201 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5825_length_6201_cov_2.468435, whole genome shotgun sequence.
ACCESSION   DAWDRC010000187 DAWDRC010000000
VERSION     DAWDRC010000187.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5825_length_6201_cov_2.468435"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1444 a         1759 c         1601 g         1397 t
ORIGIN      
        1 gctcctgatg cgccagtcgc accaccagct tctgttcgcc ccaacgtccg tgcgccaggt
       61 tatctgcgcc gaggatctcc agcgtgtcca tcaccagcgg cacaccacct tctgcctgcg
      121 agcttagcgc tatatgttcc gggcgaatgc cgagggtcat tttacgcccg gcatactgac
      181 ggtagccacc gtttagcggc agcgtaatac caccatccag ttcgaaatgc gtaccttcgt
      241 tattcacgcg gccagccagt aggttcatcg ccgggctgcc gataaaactc gccacaaaca
      301 ggctggcggg cttttcgtac acttcaactg gcgtgccaat ctgttcggcg acgccgccgt
      361 tcatcaccat tactcgctgg gcgagcgtca tcgcctcaac ctgatcgtgg gtaacataga
      421 gtgaagtcgt tttcaggcga cggtgcagct gttgcagttc aagacgcatc tgcacacgca
      481 acttggcatc gaggttagag agcggctcat caaacaggaa cactgccggc tcacgcacaa
      541 tcgcacgccc catcgccaca cgctgacgct gaccgccaga tagctcacgc gggcggcgct
      601 taagcaggcc gtccagctcc agaatgcgcg ccgcttcttt aacacgttcg gcaatttgtt
      661 gcttgcccat gccgcgaatt ttcagccccc acgccatgtt ctcctctacg ctcatatgcg
      721 gatagagcgc gtagttctgg aacaccatcg caatcccgcg atctttcggc tccatttcgg
      781 tgacacgctt gcggtcgatc cagatatcgc ccgtcgttac ccgctccagt ccggcaacca
      841 ttcgcaataa cgtcgatttc ccgcagccag acggcccgac catcacgata aattcgccat
      901 ccgccacatc aagggtcagc ggtttaatga cctgggtttt gccatcccag cttttggtta
      961 ctgcctgtaa tttcagtcct gccatcttat ttctcactat cgaccaggcc gcgcacgaag
     1021 gcacgctgca tcactaaaac aatcactact ggtgggatca gggttaacaa catcgccgcc
     1081 atcaccgagt tccattccgt tgtgccttcg cctgtggcga tcatcccttt aatccctgcc
     1141 acggtggtgc cgagatcaac gtcggtaata atcaacaacg gccacaaata ctggttccag
     1201 ccgtaaataa aggtgatcac aaacagcgcc gccagattag ttttggacag cggaaaaacg
     1261 atgtcgcaaa agaaacgcat tggcgacgcg ccgtcgatcc gcgcggcttc caccagttca
     1321 tccggtaggg tcataaagaa ctggcgaaac agaaacgtgg cggtcgccga ggccatcagc
     1381 ggcagcgtta aacctgcgta gctgtcgagc attttcaggt tggcgatgac ttccaccgtc
     1441 gggaagatac gcacttcaac cggcagcatc agggtgataa aaatcatcca gaagaagagg
     1501 ttacgtagcg gaaaacgaaa ccagacaatg gcaaatgccg agagcatcga gacggtaatt
     1561 ttgccgagcg taatgctgaa cgccatcaca aagctgttaa gcaacatccg ccagaacggc
     1621 gcgctattcg tgcctacccc gtttacccag atgttgtgga tgttttccag cagatgtgtg
     1681 ccggggatta gcgtcatcgg tgcggcatag acttcttgtt tatccagcgt cgccgcgaca
     1741 aacgccacgt acagcgggaa gaggatcacc gcgatcccga ggatcagcat ggtatggctg
     1801 aatattgtca gccacggacg gttctcaatc attggtaacg caccttgctt tcaacatagc
     1861 ggaactgcac caccgtcagc acgatgacga ggaacatcaa caccaccgac tgcgcggcag
     1921 acgaagccaa atccagtccg gtaaagcctt cacggtaaat cttataaatc agcgtcgtgg
     1981 tggcctgtac tggcccgccg gacgtggcgg cgtcgatcac cgggaaggtg tcgaagaagg
     2041 catacaccag gttcaccacc agcaggaaga aactcaccgg agcgataagc ggcagcgcaa
     2101 tcttaaagaa gcggcgaatc gggccagccc catcaatggc tgcggcttcg atcaacgaac
     2161 gggggatgga ttgcagcgcg gcatagaaga acagaaagtt gtagctgatt tgcttccaca
     2221 ctgaggcaaa caccaccagg aacatcgcct gaccgctgtt ttgcgcgtgg ttccagtcgt
     2281 agccgaactc agcgagaaaa tgggtgatca gcccgcgacc agggttaaac agaaagatcc
     2341 acaatacggc ggcaacggcg ggagccacgg catacggcag cagcatcaaa gtttgataga
     2401 aacgactgcc gcgcacgatg tactccacca gcgctgcgaa gaacagcgat accagcaaac
     2461 cgctgacggt gacaaaggtg ctgaatttta tcgtcgtcca gaaggcgtcg agatagtagc
     2521 tgtcgtgaaa cagcgtgacg aaattatcca ggccgacaaa ctggctggag aaaccaaacg
     2581 gatcgacgct ttgtagcgag taccacaacg cttcgcccgc aggccagata aaaaagataa
     2641 cggtgatgat gagctgcggc gcgaccagca aataaggcag ccagcgcgag cggaacaccg
     2701 gacgggatga tgacattaca gttaattcct gaactgtgcc ggatgcgctt cgcttatccg
     2761 gcctacacgc gatgccaggt cagataagcg aaacgcatcc gacattacac tgattaagac
     2821 ttcgtcgatt tctcaaagcg gcgcagtaac tggttcccac gctcaacggc agtatccagc
     2881 gcctgctgtg gtgtcttctt accggtccac acgctctcca gctcttcatc cacaatcacg
     2941 cggatctgcg gcatgttgcc cagacgcagc cctttggtga acggcaacgg tggcttgttc
     3001 agcatctgac gcgtggcaat atccgctcct gggtttttct cgtaaaagcc ctgctcacgg
     3061 gtcaggtcat acgccgcttt ggtgattggc agatagccgg ttttctgatg ccactcggca
     3121 gcgttttctg gctttgcgag gaagtcgagg aacttcgcca cgccggtgta ggtttcttta
     3181 tctttaccct gcattaccca tagactggct ccgccgataa tggcgttttg cggcgcgtct
     3241 ttcgcatcgg cgtcgtaagg catcatgcct acgccatagt taaatttggc gtactcgcga
     3301 atgttggcaa gagaaccgga agaggcggtc gtcatcgcgc aatcaccgtt atagaacttc
     3361 tcggtggatt catccttacg cccaacgtag ctgaaatcgc ccttcttgtt catctcttcg
     3421 agcatggcga tgtgtttcac ctgctccggc ttgttgaact caagcaccgc gtctgtgccg
     3481 tcaaagccgt tgtttttgct ggcaaacggc agaccgttcc aggcgctaaa gttttccagt
     3541 tggatccagc cctgccagcc gctggcgtag ccgcacttca tgccggaggc ttttagtttt
     3601 gcggcatagt ccgccagatc ctgccaggtt ttcggcggct gttccgggtc taatcctgct
     3661 ttcttgaagg cgtctttgtt gtaatagaga acgggagtcg agctgttgaa cggctgggag
     3721 agtaagtggc cggttttgct gtcggagtaa tagcctgaaa ccgtcggcac aaactgcgac
     3781 tcatcgaatt gaatccctgc ctctttaaat acgtcataca ccggtttaat ggctttcgac
     3841 gccatcatgg tggcggtgcc aacttcataa acctgcaaaa tagccggagc gttgccggta
     3901 cgaaatgcgg caatccccgc gcttaaattc tgttcgtagt tgcctttata ggtcggtaca
     3961 attttgtaat ccgggttttc ggcgttaaaa cgttgggcca gagaatccac ctctttaccc
     4021 agttcccctt ccatagaatg ccagaacgga atggtcgtca ctgcctgcgc atttgccatt
     4081 aaagccagtc cgagcgccag tgctgaagct gtataacgta acggtttcat tgattatctc
     4141 tcttgttgta ccgaatgcgc gaattcacgc gttttatgct cgcggagtaa catgacatgc
     4201 tcgaattaca gaaaaataac tattttgtta catttttaag atagtaaggt gtcagaaaga
     4261 tgacaaggcg gtgacggcgc gggcgaggga aaatgggaga tggggcacgg ataagcggaa
     4321 aaatagaagg tctgaatcaa actctacaga ttactcatcg tttcatgccg gatgcggcgt
     4381 aaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcaact cacccagtag
     4441 gcctgataag cgcagcgcat caggcaattt tacatttgtc acctgtctca aaggagtctt
     4501 ttgactcctt atcaatcaac gtgttattac ccgcctaaat atgcacttct cactgcttca
     4561 ttcgccagca gcgcgtcgcc ggtatcggag agcactacat gaccgttttc cagcacgtag
     4621 ccgcgatccg ccagctttag cgcctggttg gcgttctgct cgacaaggaa gatggtcatc
     4681 ccctgctcgc gcagttgttc gatggtgtcg aaaatttgct ggatgataat cggtgccagc
     4741 ccgagcgatg gctcatcaag caacaacaaa cgcgggttgc tcatcaaagc gcgaccaatc
     4801 gccagcatct gctgttcacc gccagacata gtgcccgctc gctgaacacg acgctcatgc
     4861 agacgtggaa acagttcata gacccacttt atgcgctcct ggaactggtc gcgttcggca
     4921 aaaaagccgc ccatcgccag gttctcttcc accgtcatcc gcgagaagac gcgacgccct
     4981 tccgggacaa tcgctaccgc ttcgcgcatg attttcgccg tctgccagtc ggtaatgtct
     5041 ttatcatcaa acacaattcg cccgctggtg gcgcgcggat cgccgcataa cgtgccgagc
     5101 aaggtggttt tccccgcccc gttcgcgcca atgagggtga caatctcgcc ctgattgata
     5161 tgcaggctca cctcatgcag cgcctggatt ttgccgtagt gggcgctgac tttgtcaaag
     5221 gacaacatga ctttttccat cttatgcctc acctaaatag gcacggatca cgtccgggtt
     5281 attacggatc tgctccggtg taccgtttgc cagcggcgtc ccctgattga ccacgtaaat
     5341 tctgtctgaa atccccatca ccagcttcat atcgtgttca atcaataaga tagtggtgtt
     5401 gtgatggtta cgcagttcgg caatcagctc atccagctct ttcgtctctt tcgggttaag
     5461 acctgctgcc ggttcgtcga gcattaaaat ctccggctgc gtcaccatac agcgggcaat
     5521 ctccagacgg cgctggtcac cataggccag gttactcgcc tgacggttgg catgttccag
     5581 caaaccaatg cgctcaagcc aggtagcggc gcggtcgagc gcttcgctct gggcgcgacg
     5641 gaaagagggg gttttcaaca ggccggagaa cagcccagtt ttcagttgct gatgctgtgc
     5701 caccagcagg ttttcaatca ccgtcatttc acggaacaga cgtacatgct ggaaggtgcg
     5761 caccacgccc atgcgggcaa tttgctgccc cggtaaacct tccagatgct gatcgcgcag
     5821 taaaatggtg ccgccggtgg gtttatagaa tccggtcagg cagttgaaga ccgtggtttt
     5881 tccggcaccg ttagggccga ttaacgagac gatctcctgc gggtacagtt caagattgac
     5941 gttgttcacc gccagcaggc cgccgaagcg catcatcagg ccgttaacag ataataatgg
     6001 ctgactcatg cctgctctcc ttttgctgcg ccgtttttca gcttgagttg cgggcgcgtc
     6061 atgggcagca agccctgcgg acgccagatc atcatcagca ccatcaaacc accgagcatt
     6121 aacatgctgt attcgttgaa atcacgcatc aactcgcgcg ataccaccag caaaacagcc
     6181 gccagaatca ctgcaaattg c
//
LOCUS       DAWDRC010000188         6164 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5857_length_6164_cov_2.372729, whole genome shotgun sequence.
ACCESSION   DAWDRC010000188 DAWDRC010000000
VERSION     DAWDRC010000188.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5857_length_6164_cov_2.372729"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1436 a         1512 c         1763 g         1453 t
ORIGIN      
        1 tgccggagat catcgaagag atgctggttt ccaccaccag tgaacgtcag ggccgtgtaa
       61 ttgaccgcga ccaggcgggg tctatggagc tgaaaaaacg tcaggggtat ttttaagatg
      121 aacaccgcac ttgcacaaca aatcgccaat gaaggcggcg tcgaagcctg gatgattgcg
      181 caacaacata aaagcctgct gcgttttctg acctgtggta gcgtcgatga cggcaaaagt
      241 accctgattg gtcgcctgct gcacgatacc cgccaaatct acgaagatca gctctcatcg
      301 ctgcataacg acagtaagcg tcacggcacc cagggcgaaa agctggatct ggcactgctg
      361 gtggacggcc tgcaagctga gcgcgaacag ggcatcacta ttgatgtggc ctaccgctat
      421 ttctctaccg agaagcgtaa atttattatc gccgacaccc cagggcacga gcagtacacc
      481 cgcaatatgg cgactggcgc atcgacatgt gaactggcga tcttactgat cgatgcccgt
      541 aaaggcgtgc tcgatcaaac ccgtcgtcat agttttatct ccacactgtt ggggatcaaa
      601 catctggtcg tggcgatcaa caaaatggat ctggtggatt acagtgaaga gacgttcacc
      661 cgtattcgtg aagattatct gacttttgcc gggcagctgc cgggtaatct ggatatccgc
      721 tttgtgccgc tctccgcact ggaaggcgac aacgtggctt cgcaaagtga aagtatgccg
      781 tggtacagcg gtccgacact gctggaagtg ctggaaaccg tggagatcca gcgagtggtg
      841 gatgctcagc caatgcgctt cccggtgcag tacgttaacc gtccgaatct cgattttcgt
      901 ggctacgccg gaacgctggc atccggtcgc gtggaagtcg ggcaacgtgt caaagtgctg
      961 ccctctggtg tggaatcaaa cgtcgcgcgg atcgtgacct ttgatggcga tcgcgaagaa
     1021 gcctttgccg gagaagcgat caccctggtg ctgacggatg agatcgacat cagccgtggc
     1081 gatctgctgc tggcggcaga cgaagcgtta ccggctgtgc agagcgcgtc ggtggatgtg
     1141 gtatggatgg cggaacagcc gctttccccg ggccagagtt acgacatcaa aattgccggt
     1201 aagaagacgc gtgctcgtgt tgatggcatt cattatcagg ttgatatcaa taaccttacc
     1261 caacgcgaag ttgaaaacct gccgctgaac ggcatcggcc tggtagatct cacctttgac
     1321 gagccactgg tgttagatcg ttatcagcaa aacccggtta ccggtgggct gatttttatc
     1381 gatcgcctga gtaatgtgac cgtaggtgcc ggtatggtgc acgaaccagt tagccaggca
     1441 actgctgcgc catctgaatt cagtgcattc gaactggaat tgaatgccct ggttcgccgc
     1501 catttcccgc attggggtgc gcgcgattta ctgggagata aataatggct ctgcatgacg
     1561 aaaacgtcgt ctggcatagc cacccagtaa ctccgcaaca acgcgaacag caccacggtc
     1621 atcgtggtgt tgtgctgtgg tttaccggcc tctctggttc cggtaaatca accgtcgcgg
     1681 gggcgctgga ggaggcgtta cataaacttg gcgtcagtac gtatctgctg gatggcgaca
     1741 acgttcgcca cggattatgc agcgatctcg gttttagcga tgccgaccgt aaagagaata
     1801 tccgtcgcgt cggtgaagtg gcgaatttga tggttgaagc cggactggtg gtgctgaccg
     1861 catttatctc gccacaccgc gccgaacgcc agatggttcg cgaacgcgta ggagaagggc
     1921 gctttatcga agtgtttgtc gatacgccgc tggcgatttg cgaagcccgc gatccaaaag
     1981 gtttatataa gaaagcgcgt gccggtgaac tgcgcaactt tacgggaata gattccgttt
     2041 acgaagcgcc tgaatcggca gaaattcatc tcaatggtga acaattagta acaaatttgg
     2101 tacagcaatt attagatctg ttgagacaga acgatattat cagatcctga gacaccgccg
     2161 ggtttccatg cccggttcgt caaagtaaca ggatcagata tgcgtaacag ccacaacatt
     2221 acattaacaa ataacgacag ccttactgag gttgaagaca ccacatggtc gctgccaggc
     2281 gctgtggttg gttttttatc gtggctgtgc gcgttggcaa ttccgatgtt gatttatggc
     2341 tctaacacgc tgctcttttt tatctacacc tggcctttct ttctggcgct gatgcccgtc
     2401 gctgtagtgg tggggattgc actgcattca ttgatggacg gaaagctacg ctacagtatt
     2461 gttttcactc tggcgacggt tggcattatg tttggtgcgc tgtttatgtg gctactgggt
     2521 taattgttct ttcctctcta ttctgttcat aattcaatcc gtaactaata atgagattat
     2581 gttctgcacg ccctgggtat acgtaacaat ggacaaatgt ggtacatttg cccgcgttgt
     2641 cgcggtatcc ccaacagagg atgtagagtc gtcttcggat gcatgggatg aagatgccgt
     2701 ttttcagggg gcaggatggg taaactaacg ctgctgttgc tggctattct ggtctggcta
     2761 cagtattcgc tgtggttcgg taagaacggt atacatgact atactcgcgt caataatgat
     2821 gtggcggcac agcaagctac aaacgcgaaa cttaaagcgc gaaacgatca gctttttgcc
     2881 gaaattgacg atctcaatgg cggccaggag gcgctcgaag agcgtgcgcg taatgaactc
     2941 agcatgacca ggccgggcga aacgttttat cgtctggtgc ctgacgcgtc aaagcgcgca
     3001 cagtctgcgg ggcaaaataa tcgataaatc agcccgggaa ttaacatggc aaccactcat
     3061 ttggatgttt gcgccgtggt tccggcggcc ggatttggcc gccgaatgca aacggaatgt
     3121 cctaagcaat atctctcaat cggtaatcaa accattcttg aacactcggt gcatgcgctg
     3181 ctggcgcatc ccagggtgaa acgtgtcgtc attgccataa gtcctggcga tagccgcttt
     3241 gcacaacttc ctctggcgaa tcatccacga atcacggttg ttgatggtgg tgaagagcgt
     3301 gccgattccg tgctggcagg tctgaaagcc gctggcgacg cgcagtgggt attggtgcat
     3361 gacgccgctc gtccttgtct gcatcaggat gacctcgcgc gattgttggc gttgagcgaa
     3421 accagccgca cgggagggat cctcgctgcg ccggtgcgcg atacgatgaa acgtgccgaa
     3481 ccgggcaaaa atgccattgc tcataccgtt gatcgcaacg gcttatggca cgcgctgacg
     3541 ccgcaatttt tccctcgtga gctgttacat gactgcctga cgcgcgctct aaatgaaggc
     3601 gctaccatta ccgatgaggc ctcggcgctg gaatattgcg gttttcatcc acaacttgtt
     3661 gaaggccgcg cggataacat caaggtaacg cgcccggagg atttggcgct ggccgaattt
     3721 tacctcaccc gaaccatcca tcaggagaat acataatgcg aattggacac ggttttgacg
     3781 tacacgcctt tggcggtgaa ggcccaatta tcattggtgg cgtacgcatt ccttacgaaa
     3841 aagggctgct ggcgcattct gatggcgacg tggcgctcca tgcgttgacc gatgcattgc
     3901 ttggcgcggc ggcgctggga gatataggca agctgttccc ggataccgat ccggcattta
     3961 aaggtgccga cagccgcgag ctgttgcgcg aagcctggcg tcgtattcag gcgaaaggtt
     4021 atgccctcgg caacgtcgat gtcactatca tcgctcaggc accgaagatg ttgccgcaca
     4081 ttccacaaat gcgcgtgttt attgccgaag atctcggctg ccatatggat gatgttaacg
     4141 tgaaagccac tactacggaa aaactgggat ttaccggacg tggggaaggg attgcctgtg
     4201 aagcggtggc gctactcatt aaggcaacaa aatgattgag tttgataatc tcacttacct
     4261 ccacggtaaa ccgcaaggca ccgggctgct gaaagccaat ccggaagact ttgtggtggt
     4321 ggaagatttg ggctttgagc ctgatggtga aggtgagcat attctggtta gaatcctcaa
     4381 aaacggctgc aatacccgtt ttgtggcgga tgcactggcg aaattcctga aaattcatgc
     4441 ccgtgaagtc agcttcgctg ggcaaaaaga taaacatgct gttacggaac agtggttatg
     4501 cgctcgcgtg ccgggcaaag agatgcccga tctgagcgcc tttcaactgg aaggctgcca
     4561 ggtgctggag tatgcgcggc acaagcgcaa gctgcgttta ggggcgctga aaggtaacgc
     4621 ctttaccctg gtactacgcg aagtgagcaa tcgcgatgac gttgaacaac gtctgatcga
     4681 tatttgcgta aaaggtgtac cgaactactt cggtgcccaa cgttttggga ttggcggtag
     4741 caacttgcag ggtgcgctgc gctgggcgca aaccaatact ccggtgcgcg atcgcaataa
     4801 acggagtttt tggttgtcgg cagcccgcag tgcgttgttt aatcagattg ttgctgagcg
     4861 cctcaaaaaa gcagacgtta atcaagttgt tgacggcgat gcgctacaat tagccggacg
     4921 tggtagctgg tttgtcgcaa ccaccgaaga actggcggaa ttacagcgtc gcgtcaacga
     4981 taaagagcta atgataaccg ccgcattgct gggcagtggc gaatggggaa ctcagcgtga
     5041 agcgctggca ttcgaacaag cagctgtcgc cgaagaaact gaattacaaa ccttactggt
     5101 gcgcgaaaaa gttgaagccg cgcgcagagc gatgctgctg tatccgcaac aattaagctg
     5161 gaactggtgg gatgacgtca ccgtagaaat ccgtttctgg cttccggcgg gaagttttgc
     5221 aaccagcgtt gtcagggaac ttatcaacac aacaggtgat tatgcgcata ttgctgagta
     5281 atgatgacgg ggtacatgca cccggtatac aaacgctggc gaaagccttg cgtgagtttg
     5341 ctgacgttca ggtggtcgcc cccgatcgta accgcagcgg cgcttctaat tctctgacac
     5401 tggaatcctc cctgcgcacg tttacctttg aaaatggtga tattgctgtg caaatgggaa
     5461 ccccgaccga ttgcgtctat cttggcgtaa atgctctgat gcgcccgcgc ccggacattg
     5521 ttgtgtccgg aattaacgcc gggccgaatc tgggggatga tgttatttat tccggtacgg
     5581 tagcggccgc gatggaaggc cgccatttag gttttccggc gcttgccgtc tcgcttgacg
     5641 ggcataaaca ttacgacact tccgcggcgg taacctgctc aattttgcgc gcactgtgta
     5701 aagagccgct gcgcaccggg cgtattctta atattaacgt tccggattta ccattggatc
     5761 aaatcaaagg tattcgcgtg acgcgctgcg gtacacgaca tccggcagat caggtgatcc
     5821 cgcagcaaga tccgcgcggc aatacgctgt actggattgg cccgccgggc ggtaaatgtg
     5881 atgccggtcc ggggaccgat tttgctgcgg tagatgaggg ctatgtttcc atcacgccgc
     5941 tgcatgtgga tttaactgcg catagcgcgc aagatgtggt ttcagactgg ttaaacagcg
     6001 tgggagttgg cacgcaatgg taagcagacg cgtacaagca cttctggatc aattacgtgc
     6061 gcaaggtatt caggatgagc aggtgctgaa tgcacttgcc gccgtgccgc gtgaaaaatt
     6121 cgttgatgaa gcgtttgaac aaaaagcctg ggacaatatc gcct
//
LOCUS       DAWDRC010000189         6050 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5968_length_6050_cov_2.069058, whole genome shotgun sequence.
ACCESSION   DAWDRC010000189 DAWDRC010000000
VERSION     DAWDRC010000189.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6050)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5968_length_6050_cov_2.069058"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2617..2626
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5105..5131
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1588 a         1540 c         1498 g         1387 t
ORIGIN      
        1 aatagtcagc gggaagccca acgaggcacc cagtccccaa agtacaacag acaccccagc
       61 gacccaggcg ctatcgacaa aaataatcag cccaataccc aacgccccca ttagtgcact
      121 ggcccgaacc acggcaacgc gactgtaacg gtcaatgaac caaccgccgg taaagcgtcc
      181 aacggtcatc cccagggtaa acccggcata aatcagcgag ccggaagtag ggctaaaacc
      241 gtgaccatca accattaata agggtaacca gtcgttggca gaaccttcgg caaaggccat
      301 cgccagcacc acaacaccaa tcagcagcaa ctggatatcg cgataaaaag gtacgccttt
      361 ttcaccatgc tgggtgccat cggcagcatt tttgcccgta ccgtcaggga ttgcctgaat
      421 ggcgatataa ataggcgcga tgcctaccag cgccgccaat aaaatgtgca ccgttgccgg
      481 aacgccaaag gccgtcagtg ccatcccgac acctgcgcct gccagcgtgc ccaggctata
      541 aaaaccgtgc atcatcggta aaaccgtctt attcatttct ctctcaacgg cggcaccttc
      601 aacgtttatc gccacttccg cagaaccaaa actcgcccca aagacgccga gaccaacggc
      661 aaagagcaag ggcgatgtca gccagagtgc cagacttagt atcatcatcc caatcaatgc
      721 gcaggacatc gtgacaagga tgacattacg cgtcccaaag cgtttcacta accacgctga
      781 gcagagaata ccgctcatcg aaccgatcga cagaccaaag agaacaccgc ccatttcagc
      841 gatcgagaca gagagaatat cgcggatagc aggcgtacgg gttgcccagg acgccattaa
      901 caggcctggc aaaaagaaga acataaacag cgcccaggtt cggcgtttca atgcattacg
      961 tgaagaattt acggtcatag accacgtcaa aataagaaga gggaagacaa cattagcaag
     1021 actgtgtaca tttgtacaca attgcagaga gaggaaatga catgcgtcgc gctaacgatc
     1081 cgcaacggcg agaaaaaatt atccaggcca cgctggaggc ggtgaaactt tacggaatac
     1141 atgctgttac acaccgcaaa attgctgccc ttgccggggt gccgttggga tcgatgacct
     1201 actatttttc aggaattgat gagttgttgc tggaggcgtt cagccgtttt actgagatca
     1261 tgtcccagca atatcaggca ttttttagcg atgttagtga tgctccaggc gcatgccagg
     1321 ctatcaccga tatgatctac agctcacagg tcgcaacgcc ggataatatg gagctgatgt
     1381 accagctcta cgcgctggct agccgaaaac cgctcttaaa aacggtaatg caaaactgga
     1441 tgcagcgcag tcaacaaacg ctcgaacaat ggtttgaacc cggaaccgcc cgcgcgcttg
     1501 atgcgtttat tgaggggatg acgctgcatt ttgtcaccga ccgtaagccg ctatcacgcg
     1561 aggagatttt gaggatggtt gagaggattg ctgggtagta gataaatttc aggcaacaaa
     1621 aaacccatca accttgaacc gaaatggcgg ggttgatggg ctccacaaaa tggggacatc
     1681 aaagaaaagc agtggcacta attaagactg atgccctgcg gaaaagttct gcggttgtgc
     1741 aaaaaaattt cattttcagg gcaacttcag ttttatccta atcctggcca taccatgacg
     1801 atgattgtcc ctgccagcgt cagcaggacg ttggcgattg cataggtgcc cgcatagccc
     1861 agcgccggga tgttactgcg agctgtatca ctgatgatct ccattgccgg cgcgcaggtg
     1921 cgtgcgccca tcattgcgcc gaacaacagt gcgcggttca ttcgcaatac ataagcaccg
     1981 aacaagaaac agataaccac tggcacaaga ctgacaatca atccggcaat caacatctga
     2041 ccgccaatcg cgcccaggcc gttattaata ccgctaccgg cgctcagacc aacgcctgcc
     2101 ataaacacca ttaagccgaa ctctttcacc atgcttaatg caccctgcgg aatgtaaccg
     2161 aaggtcgggt ggttagcgcg cataaagccc agcataattc cggcgaataa caacccggca
     2221 gcgttcccca tgccgaaact gaatgtgctg aactggaagg tgatcatccc gatcatcagc
     2281 ccaataacaa agaaggcgca gaatgccagc aagtcagtga cctggctgtg aatcgagata
     2341 aagccgatgc gatcggcgat ggtttttacg cgacgggcat cgccgctgac ttgtaaaacg
     2401 tcacctttgt taagcacgac gttgtcatct atcggcatct caatctggct acgaatgacg
     2461 cggttaagga agcaaccgtg atcggtcaac ttcagttgtg cgagacgttt gcccacggcg
     2521 ttatggtttt taacgaccac ttcttcagtg acgatacgca tgtcgagaag gtcacgatcg
     2581 aaaacttctt tgccgttacg gaagctggga tcgagtnnnn nnnnnngagt cgtgcgtggg
     2641 cgtcgggata gcctaccaac gctatttcat cgcccatttg tagcacagca tcaccgtctg
     2701 gattcgccag aatcccgtta cgtcgaatac gttcaatgta gcagccagtt tgtcgataaa
     2761 tacccagttc acgcagattt ttgccgtcgg tccaggccac cagctccggg ccgacgcggt
     2821 aggcgcggat caccggtaaa taaaccttac ggttggcatc agtgtccagg ccacgttcgc
     2881 gggcgatttg ctgggcgctg gtctgtaagt cctgatgctg caatttcggc aagtaacgcg
     2941 caccaacaat caaactcacc agaccgatta aataggttaa ggcatacccg aggctcagat
     3001 tatccagtgc cagtgagagc tgcctgcttt ccatgccgga atgacgcagt gtatcgccag
     3061 caccgaccag aaccggtgtc gacgtcatag agccagctaa cataccggcc gtcaggccaa
     3121 tatcccagcc aaacagctta cctaacccta aggcgatcac cagcgcactg ccaaccatca
     3181 ccagtgctaa cattaggtaa tttttcccat cgcgaaaaaa aatggaaaaa aagttcggtc
     3241 cggcttcgac cccgacgcag aaaataaaca gcataaagcc aagattaagc gcgtcggtgt
     3301 taatgctgaa atgttgttgg cctaataaca gcgatacgac taaaacgcca atggaattac
     3361 ccagttggat cgaaccaagt cgtaatttcc cgagacatag cccaagcgcg aggaccacaa
     3421 ataataacag aatgtaattc ccatttaaca attcggcgac gtttatattc acggaggcta
     3481 acttcttgtt tactagtaag ctgttgaaag aaatggtaat ttacgataat gttttttacc
     3541 agaattcagg gcgcagattc attcagcgca cctaaacgat agtaaagtaa caatatattt
     3601 tactagtgta atcacattag gtatcaacgg ctatatgaat tgcgttggcc tatattagca
     3661 tggaatgcga agcggcttta tcttactgaa cgccacactg gcgaaaaatg tgttcgatag
     3721 acgcagtgtc aggaggaacg agtgaaacat aaacaacgtt gggcgggggc aatctgctgt
     3781 tttgtcctct tcattgtggt gtgtcttttt ctggcgacgc acatgaaagg cgcttttcgg
     3841 gctgccgggc atcctgaaat cggtttgcta tttttcattc ttcctggtgc agttgccagc
     3901 ttcttttcac atcgtagaga agtcctgaaa cctctgtttg gcgcaatgct ggcggcaccc
     3961 tgttcgatgc tcattatgcg gctgtttttt tcaccgacgc gctcattctg gcaagagctg
     4021 gcatggttat taagtgcggt gttctggtgt gcgctggggg cattgtgttt cttatttatc
     4081 agtagtttgt ttaaaccaca gcacagaaaa aatcaataaa gccctcaacg cgagggcttg
     4141 tcagacgatc aggcgtccag attctctttc gcccatgcag caaaatcggt atagccgccg
     4201 atatgttgct gatcgacaaa aatctgcggc acggtttcta cgggtttacc tgccttttgt
     4261 tgtagatctt ctttagtgat cccttccgca cgaatatcta catactgata ctggaaatca
     4321 tcgcgttcat tgctcaattt ctcagccaga tcttttgcac gcacacagta agggcaaccc
     4381 ggacgaccaa aaataacggt ttgcattatt tctctcctca tagatttatg cctgtaatga
     4441 tcacgctaaa atgtattcgc tgaaagtagg tttaacctgt tgcattaatt gctaaaagct
     4501 ataactgtta aacacaatac agtgaaaagt tttagactga aggctcactt tgcagaggga
     4561 agcgtatgtg cgcgatcggt aaattgccta aaagcgtgtt gatactggaa tttatcggaa
     4621 tgatgttgct ggcggtggcg ctgctgtcgg taagcgactc cctgtcgcta cctgagccat
     4681 tttctcggcc agaagtgcag attctgatga tttttctcgg tgttttgctc atgcttcccg
     4741 ctgcggtggt ggttattctt caggtggcaa aacgtcttgc cccacagctg atgaaccatc
     4801 caccgcaata ttcacgttca gaaagagaaa aagataatga cgccaaccat tgaacttatt
     4861 tgtggccatc gctccattcg ccatttcact gatgaaccca tttccgaagc gcagcgtgag
     4921 gcgattatta acagcgcccg tgcgacgtcc agttccagtt ttttgcagtg cagtagcatt
     4981 attcgcatta ccgacaaagc gttacgtgaa gaactggtaa cgctgaccgg cgggcaaaaa
     5041 cacgtagcgc aagcggcgga gttttgggtg ttctgtgccg actttaaccg ccatttacag
     5101 atcgnnnnnn nnnnnnnnnn nnnnnnnnnn ntgggatggg gcggggtata tatcggcggc
     5161 ctgcgcaata atattgaagc ggtgacggaa ctgctgaaat taccgcagca tgttctgccg
     5221 ctgtttgggc tgtgccttgg ctggcctgcg gataatccgg atcttaagcc gcgtttaccg
     5281 gcctccattt tggtgcatga aaacagctat caaccgctgg ataaagacgc actggcgcag
     5341 tatgacgagc aactggcgga atattacctc acccgtggca gcaataatcg ccgggatacc
     5401 tggagcgatc atatccgccg aacaatcatt aaagaaagcc gcccatttat tctggattat
     5461 ttgcacaaac agggttgggc gacgcgctaa aactgccacg tcgatgtatg atacgcgggc
     5521 ttttgaccag gtctgacaga gaggtgcagg gtgaaaattg ccatattgtc ccgggatgga
     5581 acgctctatt cgtgtaagcg gctgcgtgaa gccgctatac agcgcggtca cctggttgaa
     5641 attcttgatc cgctttcttg ctacatgaac ataaatcctg cggcgtcttc tattcactac
     5701 aaaggccgca agttacccca ttttgacgca gtgatcccgc gtattggcac cgccattacc
     5761 ttttatggga cggcggcact gcgccagttc gagatgctgg ggagctatcc gctcaatgag
     5821 tcggtcgcca ttgcccgggc gcgtgacaaa ttgcgttcca tgcaactgct ggcgcgtcag
     5881 ggcatcgacc tgcctgtcac gggcattgcg cattcgccgg atgataccag cgatttaatc
     5941 gacatggtcg gtggtgcgcc gctggtggtc aagctggttg aaggcacgca gggaattggc
     6001 gtcgtgctgg cggagacgcg acaggcggcg gaacacgtca atcacgcggt
//
LOCUS       DAWDRC010000190         6033 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5988_length_6033_cov_3.244563, whole genome shotgun sequence.
ACCESSION   DAWDRC010000190 DAWDRC010000000
VERSION     DAWDRC010000190.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6033)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6033)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5988_length_6033_cov_3.244563"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1578 a         1437 c         1622 g         1396 t
ORIGIN      
        1 aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc
       61 cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgatttc
      121 aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt
      181 tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct
      241 aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt
      301 aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa
      361 ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt
      421 gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac
      481 tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa
      541 gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg
      601 gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg
      661 tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc
      721 gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg
      781 tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa
      841 gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac
      901 catcgctgtc cataatggtc gtcagcatgt tccggtattt gtaaccgacg aaatggtcgg
      961 tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa
     1021 agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct
     1081 cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg
     1141 caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt caagaaagtt
     1201 ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa
     1261 gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa
     1321 ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc
     1381 tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta
     1441 ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg
     1501 acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc
     1561 gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg
     1621 gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca
     1681 tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa
     1741 aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta
     1801 tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta
     1861 gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac
     1921 cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg
     1981 gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg
     2041 ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat
     2101 aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc
     2161 gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg
     2221 ttggccgtgg tcgtctgact gcacgtcaga tcgaagcagc acgtcgtgct atgacccgtg
     2281 cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa
     2341 agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg gttgccttga
     2401 ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag
     2461 cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga
     2521 tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct
     2581 gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca
     2641 gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga
     2701 gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac
     2761 aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt
     2821 aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc
     2881 ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg
     2941 gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg
     3001 gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg
     3061 cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt
     3121 agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg
     3181 cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag
     3241 gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg
     3301 atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg
     3361 tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg
     3421 gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta
     3481 tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat
     3541 gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc
     3601 ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg
     3661 gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc
     3721 aacgtagcaa tcttcaatgc gacaaccggc aaggctgacc gtgtaggctt tagattcgaa
     3781 gacggtaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta
     3841 gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg
     3901 agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg
     3961 gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag
     4021 caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa
     4081 tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt
     4141 tctttgagcg cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg
     4201 ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc
     4261 cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta
     4321 ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca
     4381 agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt
     4441 agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt
     4501 gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga
     4561 ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct
     4621 gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc
     4681 gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat
     4741 gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc aagccgcgaa
     4801 caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa
     4861 ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact
     4921 tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc
     4981 aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat
     5041 cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct
     5101 tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa
     5161 agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat
     5221 caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc
     5281 agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg
     5341 taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa
     5401 gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc
     5461 tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc
     5521 gactcagact gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc
     5581 ggatctgcgc gcctaccgtc gtcctgagcc ttacaaaggc aagggtgttc gttacgccga
     5641 cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc
     5701 tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg
     5761 cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc
     5821 tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac
     5881 cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa
     5941 aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc
     6001 actggcagat gctgcccgtg aagctggcct tca
//
LOCUS       DAWDRC010000191         6028 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5994_length_6028_cov_2.228528, whole genome shotgun sequence.
ACCESSION   DAWDRC010000191 DAWDRC010000000
VERSION     DAWDRC010000191.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5994_length_6028_cov_2.228528"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1426 a         1690 c         1508 g         1404 t
ORIGIN      
        1 taggcgcggt ggggttatat ctggcggcga tggccggact gacgttaatt gccctgctgt
       61 taacgcatga gacgcggcac cagtcgttgt agtcgtttta actgccggat gtcagacatc
      121 cggcaattat catcactttt tcatctgcga caaaattgtc ctgcactgat tagcctcccc
      181 ttcggaaggt gagatcagtg ccagcaatgc tgccgctggc gtaaccagcg ttgccagtgc
      241 cgccgcaaca gcaccacggg caatcaacgg cccggctttc accccagcct gcggattttt
      301 aaacgtccca cgcacataca gcggcgaacg cagcgtgata atgcgaatcc ctttactttc
      361 tggatcgata gttaaatcca gctgttccga agcaaaactt gccgtgccgg taacattgat
      421 caacgcattc tcggtatcaa aagcgaaaac ctgcggacgc gccacaccgt tggcaatatc
      481 cagattcgct gccgcgcagt tcacccgcac ctcatcgtca ccaaatatcg caccgacaat
      541 gtagttacca acattcagcc cgacaatctc catcaggttg cggctcacca gcccgtcatt
      601 catcaacagt ttcaggttgc cattactgtt gcctaaaagc gccgccaccg agttaccgct
      661 accgcgtagt tccgcgtcac cgttcatttc ccccagcgtc ttctgcatca gttccacatc
      721 cggcatcagt tctttcagtt tcagtcgacg agcctgaata tctgcccgcc cctgcatcgg
      781 ctttttatcg ccttccagat gaatattcgc cgcaatgctg ccgcccgcta tgccaaattt
      841 cagcggttgc aggcgcaggt cagcattttt gaggatgata tgagtagaaa gatcgctaat
      901 cggcaggctg ctgccatgct caatgcgccg ccctttgaag cgaacatcgg catccataac
      961 gtcccattta tcggtttcga agcggtcata aggcagcact ttgcccgcag gctgaacgct
     1021 tttttcgccc ttcttctgtt cagaccgttt caacttttct gcccctttcc cggaatcaac
     1081 gccaatcaac ggtcccaggt ccgccagccg caattgccgc gactcgacat caccttccag
     1141 ttttggtcgt ggcttgccgg tggtatagat cagagaaccg tggatatcgc tatcgccaat
     1201 gcgcccatta aaaccgcgat aatcaaagac cgacgatttt tcagtgtcga ttttcgctac
     1261 cagccgacca tccgtttcaa acggcggtgt atcgggcagc agaacgcccg tcagttcata
     1321 gagatcaccc agcgaatcgc cagaaaattt aagccgtaaa tcgacacccc ccatcttcat
     1381 tgggtcattc acaacgccat caaaagcaac acgggtgtta ccagagcgga aatcagcctg
     1441 taccggaaac ggcgttcctt cgccacgcag cgccagcata ccgcctattt ttcccgtacc
     1501 agtgagcggt tcaccgttat aacgtccctg cgccttcagg ccaaaaacgt aatcgcccac
     1561 cttttcttta tccgctttac ctttcgatcc ggtaacttcg ctgaacggca gcggcttgcc
     1621 taacggatcg acaaaaattt ccagatccgc tttgcttact ttgtcatcaa tggcgatccg
     1681 cccttgatcg aaaagaatat tatccagctg aaacgaccac gccgacggct ttgcattcgc
     1741 gtctttgttg tcatcgttgg tgagattaaa cgtccagtta ttgttctttt cggagaggcg
     1801 aatcaggcgc gcgtcgggct tttcgagctt gatccacggc aaccagaccg ttttggtcag
     1861 cagcgccagc ggggccagcg tcgcttctac tcgcggcaaa tgcaccattg tgacttcggg
     1921 aatatccggt ggattgccaa gaatgatgtc ttccgcgtgt acatggggcc acggcaccca
     1981 gctgcgccag ccagtttctt gtttttgccg ctcccacacc acgcccagat cgccacggat
     2041 agcgaacgga cgattcaact ccgcagagac tttctggttg atggtcggtt tgagtcgatt
     2101 ccaggcaaaa gatgcaatca aaatgatcgc cacgacaatc aacaacaaga aagcccctga
     2161 aatcgcagcg gttattttgc ctgccttgct catcgttcgc ttcctcatat tcttcctgtg
     2221 ccagtcctaa agatagtcca gccaggcgga aaatgaggca gcttatagcg ccagaaccgc
     2281 cgtattcagc gtttctatcg gtgccgggcg tgaaagaaac cagccttgtg cggcgaatgc
     2341 gggcgagttc tgaacatcac gccactcttc tggtgtttct acgccctcga caatcacccc
     2401 gcgacaatag cgattcatta gatgtaaaag ctgagaaaag agtgtgcgtc cttccggcga
     2461 ctgacgcagc atcacaaaca gttctcgcgc gattttgatg tagtcataac gcacttcact
     2521 tagtgcagag aaatttgcca tcccggtacc aaaatcatcc agccacagcg ggccaaattc
     2581 acacatcgag gcaaaggttg aatctttcgg caggcggatg tgttccacca gttcgaaacg
     2641 cagccaggga agatcctcaa tctggcgcag gatttttggt tgctgacgca gggcgatgag
     2701 cgtagggcca tcaatattga ccgatgccag cagaccgtgc tctacaaaga agtcggcttt
     2761 ttgcgccagc aaatcaatct gctctttcac aacctccatc cgatggctaa cggtgatttc
     2821 agtaaaatag cgatccggcg gcaggcgttg cgaagggttc aagggatgcg tgaccaccgt
     2881 taatagctcc acggccatta accgcccgca tgtttgatag atcggctgcc aggtgtaagc
     2941 acgctcacac tgcaaccaaa aacgccgttc ctgcaagctc tcgatgcttg cttcagggtt
     3001 gcttattcgc tggataacct gccttatcat caaagatgtc ctgtgtttaa aagtgatgct
     3061 gcccggactc gacaaagatt atcggcgcgc aagggcagaa ctttaccgcc tggccggggt
     3121 aaatttcccc actccacaac ggaatctttc aggaatacgt tctggctcaa aaaaataatg
     3181 gaacactgtt ttaatatggt tgaccagcaa accaccacag cgcaaactaa cgctaatttt
     3241 ttacagatca ggttcacgac tatgtccaaa aagattgccg tgattggcga atgcatgatt
     3301 gagctttccg agaaaggcgc ggacgttaag cgcggtttcg gcggcgatac cctgaacact
     3361 tccgtctata tcgcccgtca ggtcgagcct gcggcattaa ccgttcatta cgtaacggcg
     3421 ctgggaacgg acagttttag ccagcagatg ctggacgcct ggcgcggcga gaacgttgat
     3481 acttccctga cccaacggat ggaaaaccgt ctgccgggcc tttactacat tgaaaccgac
     3541 agcaccggcg aacgtacgtt ctactactgg cgtaacgaag ccgccgccaa attctggctg
     3601 gagagtgagc aatccgcggc gatttgcgaa gagctggcga atttcgatta tctctacctg
     3661 agcgggatta gcctggcgat cttaagcccg accagccgcg aaaagctgct ttccctgctg
     3721 cgcgaatgcc gcgccaacgg cggaaaagtg attttcgaca ataactatcg tccgcgcctg
     3781 tgggccagca aagaagagac acagcaggtg taccaacaaa tgctggaatg cacggatatc
     3841 gccttcctga cgctggacga tgaagacgcg ctgtggggtc aacagccggt ggaagacgtc
     3901 attgcgcgca cccataacgc gggcgtgaaa gaagtggtgg tgaaacgcgg ggcggattct
     3961 tgcctggtgt ccattgctgg cgaagggtta gtggatgttc cggcggtaaa actgccgaaa
     4021 gaaaaagtga tcgataccac cgcagctggc gactctttca gtgccggtta tctggcggta
     4081 cgtttgacgg gcggcagtgc ggaagacgca gcgaaacgcg ggcacctgac cgcaagtacc
     4141 gttattcagt atcgcggcgc gattatcccg cgtgaagcga tgccagcgta aaagcagcat
     4201 atgcaaaagg ctggatagag tgttacccct ctatccagct catccataaa tacctgatgg
     4261 ttgtgctaac tcatcaccat caggcattac tgtacaggtg gaatatccgt cacttcagga
     4321 tgtacgtcat ccgtggcgac agatgtggcg gcagccgcag tcgatggggc catgatttga
     4381 tcccaggccg cctgcaacgc tttcatgtta aattccggct cgcctttcgg ctgcagcagt
     4441 attaacgcca tatcattcga caactgctga cgcagatcct gatttaacat ctccaccgtc
     4501 aggctgttaa ggaaatcctg acgcagtttc tgatattgct ccggcgcgat atcgacgacc
     4561 tgattttgca acgaacgcat ccgctgcccc atcagaatat cggtatcagc tcgcgcatag
     4621 gcggcaaaca gtttctgcag ctccagtttc ttttgcgcca ctaacgcatt gaactcttct
     4681 tccggcagac ctttatcgcg aaccttcgcc agttcacgcg ccaccagatt aaggttgctg
     4741 ttcagcttgt cgtttggtga ttcgatgttg atggcacact gcgcacgcag atacagcaca
     4801 cgacagtcaa atccaagacc gatgtcttta ctgttactgg cgcttaacgc ttgctgaaca
     4861 tgccagaaca gcgcctcacg ggccaggtcc acacgccagt agcgcagcag tgcggctgat
     4921 tcacgaatcg gctgccacgg cgtatcccac atgatagata gccggtcctg acgcaccgcg
     4981 tcggtcataa tactcaccgc ttccgcacgc agcggagaaa gcgtcggcac cggagccggt
     5041 gtttcacgtt tgcctttcag ttcgccaaac gttttattga tttggtcgac gaccgagcgc
     5101 gcatccacgt ttcccaccac cagcagcgtc attgcatccg gggtgtacca tttctgatag
     5161 aaatctttaa tcttttccgc ttcaacgggt tgtttcagcg gatcggcagg atcgtgacct
     5221 aacaaggttg atcctttcag acgataacgc caccagccct ctttagtatc ggcaggccag
     5281 gttgccacca tgtcctgact ttgcaacgca tggttgatgg tttctggcgt gatagtcagt
     5341 ttgccagtgg catttgccag ataagagagc gcctctttca gcaagtcgtt acggttattg
     5401 ggcaaactca gattaaacag cgtggtgtca taagagacaa ttaccggcgg catcgggcgt
     5461 ttagggtcga tcccctgctg ccacaatgaa cgcgcctgcg ctgcgtcaag gccaccgctt
     5521 tgcgttagcg caatacgagg gatggcgtga ctgtaaccgc tctgttgtgt actttcggcg
     5581 agcgaaccgg tattaaccag caggcgaatt tcaacacgat cgctgggacg ctggggcgta
     5641 gtcagcactt gccactgtaa accgttggaa agcgtcccct gttgccatgc tggatcaggc
     5701 tggagcgcat ctgcctgcac atagccagca gtggccatca tcagcaaacc gcccgctaaa
     5761 agtcgaattt ttgtgccctg catgtgaacc cctgatcaac tatcctggta ataaaaaact
     5821 gcccgttgtc ggtcagtcta aatgacgtta aaaacacttc gtgttagacc gcaagaacat
     5881 gaaaatgtca cggaagaagt gaaataaacc tgaactcacc caggtggtaa gttcaggcac
     5941 aggggtcaat tatgcgcaaa cacccgcact cggggaaggg agtgcgggca taagtggtga
     6001 gattaagagg ataattcgtg cgttttgc
//
LOCUS       DAWDRC010000192         6027 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_5998_length_6027_cov_2.543369, whole genome shotgun sequence.
ACCESSION   DAWDRC010000192 DAWDRC010000000
VERSION     DAWDRC010000192.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5998_length_6027_cov_2.543369"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2186..2195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1676 a         1520 c         1352 g         1469 t
ORIGIN      
        1 cgcgcatcat caacagttaa gtcaggaacg cgccgctcgt gcggagctgc tgcaatacca
       61 attaaaagaa cttaacgaat ttaatccgca gcctggagag tttgagcaaa tcgacgaaga
      121 gtacaaacgt ctggcgaaca gcggtcaatt gctgaccacc agccagaatg cattggcatt
      181 aatggctgac ggtgaagacg caaacctgca aagtcaactt tacacggcta aacaactggt
      241 gagcgaattg attggcatgg acagcaaact gtccggcgta cttgatatgc tggaagaggc
      301 taccatccag attgctgaag ccagtgatga actgcgccac tactgcgatc gtctggatct
      361 cgatcccaac cgactgtttg aacttgaaca gcgcatctca aaacagattt cgctggcacg
      421 taaacatcac gtcagccctg aggcattgcc acagtattac cagtcgctac tggaagaaca
      481 gcagcaactg gacgatcagg ccgactcaca agaaacgctc gcgctggcgg taacgaaaca
      541 tcatcagcag gcactggaaa cggcgcgcgc gttacaccag caacgccagc attatgcaaa
      601 tgagttggca cagctgatca ccgatagtat gcatgcactc tctatgccac atgggcagtt
      661 tacgatcgat gttaaatttg acgagcatca cctgggcgct gacggcgctg atcgtattga
      721 gtttcgggta accaccaacc caggtcagcc aatgcagcct attgccaaag tcgcatccgg
      781 tggtgagttg tcccgcatcg cattggcaat ccaggtcatt accgcgcgta aaatggaaac
      841 cccggcactg atttttgatg aagtggatgt agggattagc ggcccaacag cggcagttgt
      901 cggcaaactg ctacgtcaac ttggcgaatc aactcaggtg atgtgtgtta cccacctgcc
      961 acaagtcgcg ggatgtggtc atcaacacta ttttgtcagc aaagaaaccg atggtgcgat
     1021 gacagaaacg catatgcaat ccctggataa aaaagcgcgg ttacaagagt tggcgcgcct
     1081 gcttggtggc agtgaagtca cacgtaacac actggcgaat gcgaaagaac tgcttgcggc
     1141 gtaaactttt ttcctgcttc acggtcagag taaacagcaa aacgccgtaa gaccggaaag
     1201 caaaaggttt taaagtgatg aaaggtctat tatcatcggc atattacaga tgagccacgt
     1261 actgctcggg cccgaaaagg aatcaaatca ctatgcgctg taaaacgctg actgctgcag
     1321 cagcagtact attgatgttg accgcaggct gttccactct ggagcgagtg gtttaccgtc
     1381 ctgacatcaa ccaggggaac tatctgaccg ctaacgacgt atccaaaata cgtgttggca
     1441 tgacgcaaca acaagttgcg tacgcattgg gtacaccgct gatgtccgat ccatttggta
     1501 cgaatacctg gttctatgtc ttccgccagc aaccaggtca tgaaggtgta acgcagcaaa
     1561 cgctgacgct aacctttaac agtagcggtg tgttgaccaa tattgataac aaacctgcgc
     1621 tgagtggtaa ctaataataa agttgctctc aaagacgtta aaaaaggtgc tcaatgagca
     1681 ccttttttct gtccgttatt tattcgctga tttttctgct cgttgcctgc gaagctcttt
     1741 cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca cactatcgct
     1801 tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga tatcggtacg
     1861 caattccagc aatccactgg cgcgaatagc ttcttcaacc gtcgcgccct cctgcagcgt
     1921 cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg caattttacc
     1981 tggcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta gccgccagct
     2041 ctttaaacac gcgaccaaag gcgagctcaa tcaacttatt ggtaaactca aagtcgagat
     2101 gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca ccaatcaatt
     2161 tcttgaacgg cccatccacc agattnnnnn nnnnncagat tcataagaat actttggtta
     2221 ctggtcaact ggttgcgggt agtaaacgtt ttgctgatcc cagccttaga gacatctacc
     2281 gccgcagtca tctgcccagg agtggactcc agaatccgac ttccggtaca acccggcaaa
     2341 aactgaggat aagactgaac gtcattcact aactgataca tttgctccgc gctgtagggt
     2401 accagtgcgg tccggctaat ctgaggcata acaatttcca tcaacaaaaa tccaacaaat
     2461 aatatcattt atcctgctgt aaaaaaaacg ctatcccggc gctgggtaac atcgggttca
     2521 tgctaagata gagccttgtc ccccgcagga ttgatatggg gtgttttcga tttcagatta
     2581 ccgatgattc acgacgctta tgacgaagaa aaaagcacat aaacctggtt cagcgaccat
     2641 cgcgcttaac aagcgcgccc gtcacgaata ctttatcgaa gaagagttcg aagcgggact
     2701 tgccctgcaa ggctgggagg ttaaatccct gcgcgcagga aaagccaata tcagcgacag
     2761 ctacgtcctt ctgcgtgacg gagaggcatt tctgtttggc gctaacatca cgccaatggc
     2821 cgtggcctcc acgcatgtgg tgtgcgatcc tacccgtacc cgcaagttac ttctcaacca
     2881 gcgcgaactg gactcattgt acggtcgcgt caatcgagaa ggctataccg tagtggcgct
     2941 ctccctgtac tggaaaaatg cctggtgcaa agtgaaaatc ggcgtcgcca aaggtaagaa
     3001 acagcacgat aaacgttcag atatcaaaga acgcgaatgg caggtggata aagcgcgtat
     3061 catgaaaaac gcccaccgtt aaacctatat aagcgatact aatcaaatcc tcaccgcgcc
     3121 tccctatccg gtggcgcgaa tgaacatctt atcgactgtc aaatctgaca cagatgtagc
     3181 catcccattg cttaatcgaa aaaaatcata ctatgcaggc gctaattcca ggatgataac
     3241 gccttttggg ctagtcatgc tgctcataaa tctggtatac ttaccgttac acattggggc
     3301 tgattctgga ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg cggttggcct
     3361 cgtaaaaagc cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc agcttaataa
     3421 cctgcttaga gccctctctc cctagcctcc gctcttagga cggggatcaa gagaggtcaa
     3481 acccaaaaga gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac ttaatcaggc
     3541 tagtttgtta gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac tgactaagca
     3601 tgtagtaccg aggatgtagg aatttcggac gcgggttcaa ctcccgccag ctccaccaaa
     3661 attctccatc ggtgattacc agagtcatcc gatgaagtcc taagagcccg cacggcgcaa
     3721 gccctgcggg cttttttgtg ccttgaattt gtcacgcgaa gtctgatgcc aactaattaa
     3781 atccgaactt tttaggcacc ttgttaggca cctcataaag ctttattgtt tttgaggtgc
     3841 ctaaaactat ggaaacccgg caatggcaag acaaaccaaa cctctatccg ttaaagaaat
     3901 cgaatctgct aaacccaagg aatcggacta cgttctctat gatggcgatg gccttgagct
     3961 acttatcaaa tccagcggga gtaaaatctg gcagtttcgc tacattcgcc ctatcaccaa
     4021 gaaacgtgcg aagaagagca taggccccta cccgtcagtt acgcttgccg atgccagaaa
     4081 ctaccggaca gagtcacgct ctctcctggc gaaacaaatc gatccacagg aacatcagca
     4141 agaacaactt cgcagttcgc ttgaagctaa aaccaatact ttccaactcg tagctgaacg
     4201 atggtggaat gtgaaaaaag ccagcgtgac agaggactat gccgacgata tctggcgctc
     4261 tcttgaaaga gatgtctttc ctgcaattgg cgacgttagc gttacagata ttaaagcaca
     4321 cacactggtt caggccgtcc aaccagttca ggccagagga gcactggaaa ccgttcgtcg
     4381 cctgtgccaa cgcattaatg aggttatgat ctatgcccaa aacacagggc tgattgatgc
     4441 tgttcccagc gttaatatcg gtaaagcctt cgagaagcct cagaaaaaga acatgcccag
     4501 cattcgaccg gatcagctac ctcaactgat gcagacaatg cgaacagcca gcattagcct
     4561 ttccacacgc tgcctgttca tgtggcaact tcttactatt acccgccctg ccgaagcggc
     4621 tgaagctctc tgggaagaag tagacataga agcgcgagag tggaagatcc ctgcagcacg
     4681 catgaaaatg aaccgcgacc atactgttcc attgtcagat gaagcaattg cgatactgga
     4741 gacgatgaag ccattaagtg gaaatagaga atttatcttt cccagccgca tcaagccaaa
     4801 ccagccgatg aacagtcaga ccgtaaacgc atcgctaaaa cgcgcaggtt ttggtggggt
     4861 gctcgtttca cacggcctgc gatctatcgc cagtacagcc cttaatgaac aaggctttcc
     4921 gcctgatgtc attgaggcag cacttgccca tgtagacaag aatgaagttc gtcgcgctta
     4981 taaccgcagc gattacctgg agctgcgtcg tccaatgatg caatggtggg ctgatttcgt
     5041 tatggctgct gatcacggaa atatgattgg agatggcatt cgaggtataa ggcttgtcgg
     5101 ctaatacaaa taaaggtata agcactcaaa atgggcgctt atacctttta tctaaattaa
     5161 aatttaccac ttctgactgg ttattttttt ctgattacat tgttatcaaa agccagttta
     5221 acgttctcaa catttcttga ccaagatttc aaaatagtat caactagggc atcctggctc
     5281 gccgttaata atcggtactt tttgctttgg ttattaatca caaaatctga ctcttcaaca
     5341 aatacccata aaggctcaat aagggcatta actgccacag ttttattttt ccacccgcct
     5401 tttggggcaa gttcgtttat aagctccact aatttattct gaataacaac gtacacttcc
     5461 gctttacttt ccccaccttt ctttccagct ttagccctag tgcgcgatag ttttttttgt
     5521 ttgtcagcat aagtcttgaa ccagatcatt ccgatgcttt tatcaaataa actagaggca
     5581 gtaataaatg ccttaagcgc aatacgttca ttctccgaca ttaccgactt tccccattga
     5641 tacgccattg aggcataaat gagatgttct acaaagacgt caacaatgat gacaggctca
     5701 cggactaaag gaaaaatagt cttttgaaaa atacgttttt ccaagtttat ccagcattcc
     5761 tgtactgggt cgtataactc tgtgtactct tctttaatct ccagtgttcc attcacttta
     5821 tgatatttat cttgccggat atgaggatcg gagtacagca tcacacgcgt ccctaccttc
     5881 tcagattccc aatattgttt cctcattatg gctagttctt cttgcatcac tgggtaagtt
     5941 tcatcaagca cttcagcata tctcttccac tcacaatgag acatctcacc agctagctca
     6001 cttctttttc gacgaatttc ttcacac
//
LOCUS       DAWDRC010000193         6019 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6006_length_6019_cov_2.744131, whole genome shotgun sequence.
ACCESSION   DAWDRC010000193 DAWDRC010000000
VERSION     DAWDRC010000193.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6006_length_6019_cov_2.744131"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    936..945
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1676..1685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1411 a         1554 c         1500 g         1534 t
ORIGIN      
        1 gatgcgctga cgcctgccga tcgctatctc gaactgttcg agcatgttca gtcggcgaaa
       61 attttccccg acagtaaaac ctttcccgac tgcgcaccca aaatggaccc gctggatatt
      121 ttaatccgct accgtaaagt gcgccgtcat cgtgattttg acttgcgcaa gtttgttgaa
      181 aaccacttct ggctgccgga ggtctactcc agcgagtatg tatcggaccc gcaaaattcc
      241 ctgaaagagc atatcgacca gctgtggccg gtgctaaccc gtgaaccaca ggatcacatt
      301 ccgtggtctt ctctgctggc gctgccgcag tcatatattg tcccgggcgg ccgttttagc
      361 gaaacctact attgggattc ctatttcacc atgctggggc tggcggaaag tggtcgggaa
      421 gatttgctga aatgcatggc cgataacttc gcctggatga tcgaaaacta cggccacatc
      481 cccaacggca accgcaccta ttatttgagc cgctcgcaac caccggtttt tgcgctgatg
      541 gtggagttgt ttgaagaaga tggtgtacgc ggcgcgcgcc gctatctcga ccacctgaaa
      601 atggaatatg ccttctggat ggacggtgca gaatcgttga tccctaatca ggcctatcgc
      661 catgttgtgc ggatgccgga cggctcgctg ctcaaccgtt actgggacga tcgcgacacg
      721 ccgcgtgacg aatcctggct tgaggacgtt gaaaccgcga aacattctgg tcgcccgcct
      781 aacgaggtgt accgcgattt acgtgcgggg gcggcctccg gttgggatta ctcttcccgt
      841 tggctgcgtg atactggtcg tctggcgagc attcgtacca cccagttcat ccccatcgat
      901 ctgaatgcct tcctgtttaa actggagagc gccatnnnnn nnnnngcgcc atcgccaaca
      961 tctcggcgct gaaaggcgag aaagagacag aagcactgtt ccgccagaag gccagtgccc
     1021 gtcgcgatgc ggtaaaccgt tacctctggg atgatgaaaa cggcatctac cgcgattacg
     1081 actggcgacg cgagcaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg
     1141 gtatggcgaa ccatgaacag gccgatcgtc tggcaaacgc cgtgcgcagc cggttactga
     1201 cacctggcgg gattctggca agcgagtacg aaaccggtga acagtgggat aaacccaacg
     1261 gctgggcacc gttacaatgg atggcaattc agggatttaa gatgtacggc gatgaccttc
     1321 tgggtgatga aatcgcgcgc aactggctga agacggtgaa tcagttctat ctggaacagc
     1381 acaaactgat cgagaaatac catattgccg atggtgttcc tcgcgaaggc ggcggtggcg
     1441 agtatccgtt gcaggatggg tttggctgga ctaacggtgt ggtacgccgt ttaattggtt
     1501 tgtacggcga accataatat ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt
     1561 atcctcttca ggaggagata tttaacatca ttgccgcctg ggtgcgattt ttgacttcca
     1621 gacggcgata cagtgattcc agatgcgctt ttaccgttcc ggtactgata ttcagnnnnn
     1681 nnnnncttta tttgattcgc ccgccgctaa catggttaaa atctcccgct ggcgggcgct
     1741 taacgatttg agatctttaa tgtccttttc cggcgtcgtt cgccagtctc caggcagaaa
     1801 catcatcccc atcgccgcac tatttaccgc caacgcaaat gtctcgacgg ttgaatcacg
     1861 aggcacaatg gccagcacat taaaatgaat aacttcctgt aaccaccgtt tgttgcaatc
     1921 cgtcgccgta attaacacct taacctcagg aaattgcacc acggtttttt gcagcaacca
     1981 gtagcaaaac tcaccatcct gatcgccatc gagcatcact aaggcttcag ggtaactttc
     2041 cagcttttgc cataactcgt ctgcctgact ggccccctga atactcactc ctggaatacg
     2101 atgctgtaaa ctgattttca ttccatgaat aaatattgac tgcctgtcaa acatgactat
     2161 ttgcataact gaatctccac ctgaatacgt taaaaagact taagtagtgg aagggtatta
     2221 ctcgcgagaa aaaataagaa ttcgccattt ggcggtggcc attctacaga gataacgcgt
     2281 agaaaatagt taccgatata aatagttaca gctaaacgcc tgaaattaca tgtcgagggc
     2341 actatttaaa acatttttga ggatttcctt atatcggtaa ttagtacgca tgcaattaaa
     2401 aatgaaattc cgcgaccata aacaaaaata acaaacggca atgagataaa aataagcaca
     2461 aatagccaat acatcttcta ttcactttaa aaggaatcgc tgaaaaatac gctctgttta
     2521 agggaattca cctttctcat aaagccattc cgcccttttc ctgctgagaa atcgccacat
     2581 tcatcatgac aacattgtga aacccggcat tagatgttag aaaaaacaat aaacaatgcg
     2641 atatgcgcgc tactgtttcg ttgtgaacaa caatcggtca aagaaatgga taaaattcac
     2701 gcaatgcagt tgttcatcaa agtcgcggag ctggaaagtt tttcccgcgc ggcggatttc
     2761 tttgctttgc caaagggaag tgtttcgcgc cagatacagg cactggaaca tcaacttggc
     2821 acccagcttc tccagcgcac cacgcgacgg gtcaaactca cgccagaagg catgacctat
     2881 tatcaacggg caaaagatgt gttgagtaat ctcagcgaac tggacggtct gtttcaacag
     2941 gatgccacca gtatcagcgg taaattacgc atcgacatcc cgtcaggaat cgcgaaaagc
     3001 ctgttactgc cgcgcttgtc ggaatttctc tatctgcatc cgggaattga gctggaactg
     3061 agtagccatg accgtccggt agatattctt catgatggtt ttgattgcgt gatacgcact
     3121 ggcgcattac cggaagatgg cgttatcgcc cgttctctcg gcaaactgac catggtcaac
     3181 tgtgccagtc cacactatct gacgcgcttt ggttatccgc aaagccccga tgatctgact
     3241 tcacacgcaa tagtgcgtta tacaccgcac ctgggtgtac atccgttagg ttttgaggtt
     3301 gccagcgtta atggcgtcca gtggtttaag tctggcggca tgttgacggt aaacagtagc
     3361 gaaaactatc tcgccgccgg tcttgccggt ctggggatta ttcagatccc gcgcattgcc
     3421 gtgcgcgaag ccctgcgtgc cgggcggctt attgaagtgt tgcctggcta ccgtgccgag
     3481 ccgctgtccc tttcactggt ttatccgcag cgtcgggagc tttcccggcg tgtaaacctg
     3541 tttatgcagt ggctggctgg cgtaatgaaa gagcacctgg actgaccgac tatacttttt
     3601 aagaatgacc acgacagaag gacaaaagag cggatgacgc aggaaaacga gatcaaacgt
     3661 cccacccagg atctggagca cgagccgatt aagcagttgg ataatagcga gaaaggcggc
     3721 aaagttagcc aggcgctgga aaccgtcacc accactgccg agaaagtcca acgccaaccg
     3781 gttattgcgc acctgattcg cgcgacagaa cgctttaacg atcggctggg taaccagttt
     3841 ggggcggcta tcacctattt ctcatttttg tcgatgatcc ccattttgat ggtgtcgttt
     3901 gccgcagggg gctttgtgct ggcctcccat ccgatgttgc tacaggatat cttcgacaaa
     3961 attctgcaaa acattagcga tccgacgcta gccgccacgt tgaaaaacac catcaacacc
     4021 gccgttcagc agcgtacgac tgtagggctt gtcggtctgg cagtggcgct ttattccggc
     4081 atcaactgga tgggtaacct gcgtgaagcg attcgcgccc agtcacgcga tgtctgggaa
     4141 cgctcgccgc aagatcagga gaagttctgg gtaaaatatc tgcgtgattt tatttcgctg
     4201 attggtttgt tgattgcgct gattgtgacg ctttctatca cctcggttgc cggttctgcg
     4261 cagcaaatga ttattagcgc cctgcacctg aacagcattg agtggcttaa accgacatgg
     4321 cggttgattg gcctggcgat ttccatcttc gccaactatc tgcttttctt ctggatcttc
     4381 tggcggttgc cgcgccaccg tccgcgcaaa aaagccctga tccgcggaac atttctggct
     4441 gctattggtt ttgaagtgat taaaatcgtg atgacctaca ccctgccatc gttgatgaaa
     4501 tcaccctctg gcgcagcatt tggttccgtg ctggggctga tggcattttt ctacttcttc
     4561 gcccgtttga cgctgttttg cgcggcgtgg attgccaccg ccgaatataa agacgacccg
     4621 cgaatgccgg ggaaaacgca gccttaaaat aggccggatg cggcgtacga atttattcgg
     4681 cctggctccc cgtaggccgg ataagatgcg ccagcatcgc atccggctat aatgcgcaca
     4741 taacctcttg aaactctttc ccagagcctc tttagccatc tattcaggag caaacaattt
     4801 cattccaact cataacccca gcatataaat ccagttggta acttttattt aacctaaaac
     4861 cagtttaatc caccatttat aaaattatgt gaagcatttc atagaagaaa aatcactggc
     4921 ctaaacatta tccccttttt gcatgatttt tgaccatttc cgcgatttgt tacacattga
     4981 aatatcactt ttgctgcgcg taatatggct attcgttagc caaaaaataa gaaaagatta
     5041 tgcaagcaac agccacaaca ctcgaccacg agcaagaata cacgccgatc aactcgcgta
     5101 ataaagtcct tgtcgcctct ctcattggca cggccattga gttcttcgac ttttacattt
     5161 acgccactgc ggccgttatt gtgtttccgc atatcttctt cccgcagggc gatccaacgg
     5221 cagcaacgct acagtcgctc gccaccttcg ccatcgcctt cgtcgcgcgc cccattggct
     5281 ctgccgtttt tggtcatttt ggcgatcgcg ttgggcgtaa agcgacgctg gtcgcctcgt
     5341 tgctaacgat ggggatttcg accgtggtga ttggtctgct gccaggctat gccacgattg
     5401 gtattttcgc cccgctgctg ctggcgctgg ctcgatttgg tcagggtctg ggcttaggtg
     5461 gtgaatgggg cggcgcggct ctgctggcga ctgaaaacgc cccaccgcgc aaacgtgcat
     5521 tgtacggctc cttcccgcag ctgggcgcac cgattggctt cttctttgcc aacggcactt
     5581 tcttgctgct ttcctggcta ctgaccgacg agcagtttat gagctggggc tggcgtgtgc
     5641 catttatctt ctcggcggtg ctggtcatta tcggcctgta tgttcgcgtg tcgctgcatg
     5701 agtcgccggt gtttgagaaa gtcgctaaag cgaaaaaaca ggtgaagatc ccgctgggta
     5761 cgctgctgac caaacatgtt cgcgtaaccg tactgggtac gttcattatg ctggcaacct
     5821 atacgctgtt ttacatcatg acggtctact ctatgacctt tagtaccgcc gccgcgccag
     5881 ttgggcttgg tttaccgcgt aacgaagtgt tgtggatgct gatgatggcg gttatcggtt
     5941 ttgcagtgat ggttccgttg gcgggtctgc tggcagatgc ttttggtcgc cgtaaaagta
     6001 tggtggtgat cactacgct
//
LOCUS       DAWDRC010000194         6011 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6011_length_6011_cov_2.306246, whole genome shotgun sequence.
ACCESSION   DAWDRC010000194 DAWDRC010000000
VERSION     DAWDRC010000194.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6011_length_6011_cov_2.306246"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    338..347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2157..2166
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2795..2804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4333..4342
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1472 a         1558 c         1395 g         1546 t
ORIGIN      
        1 tattgtacct gtcgttggca cagcgggata ttgcgctact gtgccaacgc tttactcatg
       61 ggactttagc acaggactgt agcaaaaaac gttgatgcgc caatattgct gcaccctgag
      121 cgccattaaa gtcagaagat gaggcggcaa taaagcgcac gacctgatac ggcagtggac
      181 ggcgcaggta cttttgggtc atgtcaatga gagtctcgcg tgggaaggcg ggcatatcca
      241 tcacgccacc acccagaatc accgcatcgg gatcgaacag attaatgctg gtggcaatgg
      301 ctcgtgccgc gttttcaagc agactctgga cgaaaggnnn nnnnnnnttt tccgcatgga
      361 cgaaaagatc gctcaatggg taatttcggg gctgttgttc gtaccagcgt cttagcgcca
      421 ttccagagca attggtttcc aggcacccag aattgccaca cgcgcagtgt tgggtcatat
      481 ctcccagggg gatatggccc agttcgcctg ccacaccgtg tgcacccgtc cacggcgcac
      541 cgttcatcca cactgcgaac cccatccccg taccgagata ggctgccaga accagttgtt
      601 gcgtaaggcg gttttctact acgtcccagg agagttgcag gttaacgtcg cgggaaaact
      661 ccaccggaaa attcagcgta ttttcgagct tatcggcgag atcatataaa tctgccgctg
      721 ttaacggcag gttaggcgta gaaataatgg tgcgtttatc tttactgacc agcgccggaa
      781 atcccatcac cagaccatga cagcgagcgt taaagcgcct gagttgctcg tcaatcattt
      841 cgccgatacc cgacaccagg tcgggagcaa taacttctgc ggtccgcttt ttttcgcagt
      901 gtagcgtttc accttctgct gtccgcagac aaaagcggat atgcgttgcc cccatatcca
      961 cgcccgctac gacgttatgc tgtttttgca tgaggctgta cctcgctttt tgcagccaga
     1021 atctgcgcgg tcataattct ccatgcttcg tcgatatttt ccgcatgatt aaacaggccg
     1081 gaagtgccga cgataaagac atccgcccct gccgccatta gtttttcgta agttgcctgg
     1141 ttgcaggaac cgtccacctc aatttcgtac tccagacctt ctcgttcacg ccatgccttc
     1201 agttcggcaa ctttatccag catttcagga atgaagggtt gtccggcaaa accgggatcg
     1261 acagtcatga ccgtaatttt atcagcctta tggatatagt atttcatggc ctcaaccggc
     1321 gtttccggat taagaatcag ccccactttc atgccatgac ggcggatttc atcaatcagg
     1381 cggaatgcct gaccgttgat agtttccgga tgcaaagtga tgaaatcagc tcccgcacgt
     1441 gccagttgag caatgtaatc ctgcggacgc gttaccatca gatggcagtc gagcggttta
     1501 ctcgccagtt tttttacctg gcttacgaag aacggtgaga gcgtcagatt cggcacaaag
     1561 tggccgtcca tgatatcgat gtgaaagtaa tcggcatggc tgtcgataaa ttcgatctgt
     1621 tctttaaatt tcagcagatc catacacatt aacgaggggg agattttcat gttcaattcc
     1681 ttacttactg ataagacggt caagggcgac agccgcgata attaatccgc ccatcaccac
     1741 cagttggtaa taggtttgta cctgcaaaat attcagaccg ttgttgatgg tgccgatgat
     1801 caacccgcca atcaccacag agaaaatgcg ccccttgccg ccgaagaaac tggtgccgcc
     1861 aatgatggcg ctggcaatgg tataggtttc aaaacccata ccggcaagcg gttctgcggc
     1921 accgagtcgt gcagttgaga cgacgcctgc cagacctgca caaacaccgg agatgataaa
     1981 caccaccagg atgtggaatt tcacgtcaat cccggaatag aacgccgagt ttttgttgcc
     2041 gcccagtgcg tagatgttgc gcccgagccg catacgcgtt gtcagaaacc aaaggatgag
     2101 cgcgacaatt agcgagaaga taacggggac aggtatccca attacgctgg cggcaannnn
     2161 nnnnnnggtg tagtcccgtc cagttgacca ggcagccgtt gatcgccccc agtgcgccgc
     2221 caaccagtac accgccaatt agcgccgcga gaaacggatc aatacctgcc aacatcagtt
     2281 tagcggtcac cataccggaa agcgccagaa tcgcgccaac cgaaaggtcg ataccggcga
     2341 ccaggatagc gaagaattcc cccatgccga tcagtaccgt cacggagctt tgcacaaaaa
     2401 tctgggtaat attattggtg gtcagaaaat attctggcga cagcgaacca aaaatggcga
     2461 cgatgatcgc caggataaaa aaggtgccgt atttatccca gaacagcgca aagttgaacg
     2521 gtttcttctc gctcgcttcg ctttttactc ttgtggtaaa gcccatgcca taatctcctc
     2581 ttcgctcatg tcatcgcgat tggtcaggat ttgcgtcagt cgtccttcgc agaacacggc
     2641 gatgcggtcg cagacggtga taatttcagg tagttcagat gacaccatca ggatgacttt
     2701 tccgtcgtcc gccagttggc gcatcacttt gtaaatttcg gctttcgcgc caacatcgat
     2761 gccacgggta ggttcatcga aaatgatcac ttccnnnnnn nnnngctcgt caacttcatg
     2821 aaacaacccc atcgcgcctt tatagccgcc gtctttcaga ctgcggctga tagccatgtt
     2881 ctgagcgata gaaaagttgg ggaaaaagcc gttatcccgg cggctttcag tgatgtaagc
     2941 catccctttt ttcacggcat ccaggggaga acgtggagag atatctttgc cattaagacg
     3001 gatttctccg ccagcgcgtt tatcaacgcc aaacaggcag ttcatcagct ctgtacgtcc
     3061 ggaaccgacc agcccggcaa agcctaatat ttctccccgg cagacgctaa atgagatatc
     3121 ccggaccttt tttctgtcac ggctggtgac gttgcgcacc tcaaaaaccg tatcgtgcgc
     3181 aaggttgctg acattctcct tcatcgcgtt aaaacggttt tgcaattcgc ggcctaccat
     3241 cagacggacg atatcgtcat ttgacacatc gctcaccatg ccgctgcaaa cgctgctgcc
     3301 gtctttcatc accgtatagc ggtcgcaaat acggcgaatt tccgccaatt tatgcgagat
     3361 atagacgatg gccgtaccct ccttacgcaa ctgattcatg atcagaaaca gatagtccac
     3421 ctctttattg gtgagtgagg aggtgggttc atccatgatg atgactttgg cgtcgagcat
     3481 cagcgttttg gcaatttcca gcatctgctt gtggctgata gataagttcg ccactttctc
     3541 atctaaatca actttcaagc ccacgcgtaa taacatcatg gcggcgcgga cacgcatttc
     3601 tcgccagtcg ataatattga cgccacagat ttttttcgtc agatgacgac caatatataa
     3661 attttccagt acggttaatt catcaataac gctgagttcc tgataaataa tcccgatacc
     3721 gagttgtgcc gctaatttat gatccagttt gttatagtta atgttattaa tggtaatggt
     3781 gcctttggtc ggctcatgta tcccggataa cactttcatt agcgtagatt tacccgcacc
     3841 attttctcct agtaatgcat gtatttcacc aggataaacc gttaaattaa ccgactttaa
     3901 tgcgtgaacc ggaccaaagg acttgccgat ccccgccatc gatatatatg gcgtggccat
     3961 aaataacctc tctgcacgaa atatcgtatt attgcctgat ggctacgttt atcaggtcta
     4021 cgttcaggtt attttgttgg ccggataaga cacttcatta gcatcatcat ccggcatcat
     4081 tcagttttat tgagtgacca ggattgaatc gaccagttta aattccggtg ctttatccag
     4141 cgggataacc ttgccggatt tttcagcgtc aaccatcagc ttcagacccg ttgcaccgat
     4201 atccgccggg ttctgggcaa ccgtcgcggt catttgtccg gcttccacca ttttgcgggc
     4261 ttccggaatg ccatctgtac cgacgaccaa cactttgccc gtttttccag cgtttgcgac
     4321 tgcctgagca acnnnnnnnn nnccattgcc gtcgtgtcat tcgcgcaata gatcgcttta
     4381 atattcggat tacgttgcaa tacgttagtg gcgacatcca gtgctttaat gcggtcccag
     4441 tcggcaggct ggctggcgac aagcttgatc tggcttgctt ttttgaaggc tttggtggca
     4501 ccattacgac gcgcttcacc ggaggcgtta ccggctttac cctcaatgat tgcgacttca
     4561 ccaccttcag cgcccaattt gtcaataatg aatgacgcgc ctttcgcccc gacggcaaca
     4621 ttatcggtgg tgacaaaacc ttccacatta ccgcctgctt ttttcagatt atccatgtcg
     4681 attttttcat cgagattgac cagataaatg ccttttttcc atgcgcgtgc aacaggcatg
     4741 accaggttca ctgaggataa tggtgcgaag gcgatacctt tgtaattttt attactgaga
     4801 tcttcaaata actgcaattg agattgaaaa tcgccttctg aaggagaggc aaaaatatca
     4861 acgctgacgc ccagcgtttt tgcttcatct tcaatgcctt ttttcatatc tacccaaaat
     4921 gggttggaga gtgttttcaa tacgacggca tattcggcgg cagcaaaagc gctggttgac
     4981 aacattaagc ccacgagtgt gccgctgaaa tatttcagat atttattcat aatcatgttc
     5041 tcacggtaag gggagaggta actccgcgat ggcggaatta cctttttctt atttcagcgc
     5101 gcctggtgag aagaaatcaa caattgcgcc ggttttttgc atattaatat tagcttgttc
     5161 aatgttgagc tgggcgacag agacaaaaaa cgcgtccagc aaagttagtt gtaatattct
     5221 tgccgaggca ttacgaccta ataacggtgt ttccggggcc ggtgagcaaa taatataatc
     5281 ggccagcttc gctatcggtg aatgatagct atgcgttata caaataatct ttgccccgtt
     5341 cttttttgcc agttctacag ccgcttttac atcactggtt cgcccggaat gagtcaccac
     5401 cagcaccaca tctccttcct gtaacaacga agcagacatc atcatgatgt gagcgtccgg
     5461 ataggcctgg cagcgtacgc caatgcgcaa aaacttgtgc tgtacatcag cacagatagc
     5521 atttgatcct ccggcaccgt acaaatcccg ctgtctggcc tgatagaaaa agcgggcggc
     5581 acggtggatc tcatcaacgt tgacgatcga ctgaccttcc atgatggtgc gtaaagtgat
     5641 gttaaatacc ttattcacca catcctgcgg cgcttcatca aaagccaact cggaaggcaa
     5701 tacttgttct gactgagaaa aataatcttc cagcgcactg cgtaagttac gaaagccgct
     5761 aaaccccagc agctttgata ccttaacgat catcgcttca gataccgcca gagcttctgc
     5821 gacatcttta attgcgggtg cacaactcag gttaccgggt ttgagtaacc actccacgat
     5881 gcggctttcg ttttctgtca ttccttcctg cttcattcgc aggtaaggcg ctaacccgat
     5941 accgttcgga agcgctgaat caaactctga ctggctcatc acgttctctt gtccttgaat
     6001 tgaagacgcc a
//
LOCUS       DAWDRC010000195         6010 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6013_length_6010_cov_2.369102, whole genome shotgun sequence.
ACCESSION   DAWDRC010000195 DAWDRC010000000
VERSION     DAWDRC010000195.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6013_length_6010_cov_2.369102"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3944..3953
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5056..5065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5784..5793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1277 a         1557 c         1662 g         1484 t
ORIGIN      
        1 gtgcggatct tcgccataca cgcgctcaaa cggctgcaaa tgctcgtcgg taaacggcgt
       61 gcgcttgccg aagcttggca tattggtacg caggtcgtac acccacacgt catcggtgca
      121 gttcttatcc tgatgtgggt tcgccaccgt gcctttggtg aagaacagca cgttggtctt
      181 cacgccctgc gcgtaaaaaa taccggtcgg caggcgcaga atggtgtgca gatgacactt
      241 atccatcagg tcacgacgaa tgtcggtgcc tttgccgcct tcaaacagca cgttatccgg
      301 caccaccacc gccgcacgac cgccggggtg cagcgtttcg ataatatgct gcataaagca
      361 caactgtttg ttactggtcg ggtgaacaaa ggtgcgggta atgttggtgc ctgcggcgct
      421 gccaaacggc gggttagtgg cgacaatatg cgccttcggc aggttttcac cgtcgctacc
      481 cagagtgttg cccagacgga ttgcgccgcc gtggtcgagg ttgccttcaa tatcgtgcag
      541 caggcagttc atcagtgcca gacgacgggt gccgggcacc agttcgaggc cgataaacgc
      601 gcggtggatc tggaaatcct gcgtgtcgcc atcaaggtcg tccagatcat tggtttgcga
      661 cttaacgtag cggtcggctt caatcaaaaa gcccgccgta cctgctgccg ggtcctgtac
      721 cacttcacgc ggctgcggtt tcagcagatg aataatggtt ttaatcagcg gacgcggggt
      781 gaagtactgg cccgcgccag acttggtttc gttggcgttc ttctgcaaca gcccttcgta
      841 catatcgccg aagtcatcgc gcgacttacc gtgcgcaccg ttgtaccagt ccagcgaatc
      901 catatttgtg accagttcgg tcaactgttt tggctgagta atggtggtgt tgacgttctg
      961 gaacaccgcc tgcaccagct tttgattgtc ggcgcccaga tgtaccagca ggttacggta
     1021 gaactgcaac tgctcctgac cgatgcggga tttcaggtca tcccagcggt agccttccgg
     1081 caggtattcc gcttcctgcc cggtctcttt acacattttc aaaaacagca gcgaggcgag
     1141 ttcattgacg tagttttgat aggaaacgcc gccatcgcgc aggttgtcgc acagcttcca
     1201 cagcttcgcg accagatcgt tattgttcat tgggtgttcc gtaaattaag cagcggcccg
     1261 cattgtttgg ggccgcagaa aaagaaattg ccgggggtaa tatacacaaa atcattcagg
     1321 ttgcatcaag gtggcaagcg agtgaatccc cgggagcgta cagaagtacg tgaccggggt
     1381 gaacgagcgc agccaacgca gaggcagcct gaaggatgaa gtgtatacgt gtcaggccag
     1441 ctcgtcccaa atgtaatcgc tgaatttatc gagcaggctg tcgagattat cgtcaaaggt
     1501 tctttgcagc atcgtcttcc cgccgcgacg gtggaagttg ccggttttga agacatcgtc
     1561 gtcgagcacc actttttctt tcagcgcctg cgctaaacga tcgagccagc ttaattgctc
     1621 gctgctccag tcgttttcgc ccttaatgcg cgttagcgcg tgatcgacac gttcctcaaa
     1681 cggtttcagc gcatcgccca ccgcagcgcg gcgaatatgt ccaatcagcc gggcggcgat
     1741 atcttcattg cgcgtctctt gccatgcttt gcgcagggaa gattcctcaa agtgctggcg
     1801 gtcaaaccac tcctgtagct cgaccagccc tttacgggtg agatcgcgcg ggcgattaat
     1861 caccgcctgc aatgccggct gtgcattcgg ggaacgttgc accagcgagt caaaggcttc
     1921 gaggaaatcc tgcggcgtgt cgtaatcacc gtacagcgat tttacgctca ccacttcatc
     1981 gtcgatatcg aggaagatcg gcgcatcatt caggttgttg atgtcggttt tcagcttttc
     2041 cagacgggcg ataaagccag gtagtttgtt aaagacttcg gcgctccagt gcggcccttt
     2101 ttcccgcagg cgcgaggcga agccgttaaa gttaaccccc gccgcgtcct ggcatagctc
     2161 atccagacga cgcacctgtt tgtctatcgt ttcgctgcgg tcacggttaa acgtggccag
     2221 gccgatgatg cgctggagct tcgccaccag ttgttcatgg ctgtgctcgg caaaactgcg
     2281 gccatccgct tcggtgattt tataggtttc tgaatcggta atttcgttga ccagcgtttg
     2341 cagttccacc ttcggacgca ccaccaccgg acgcatggtg tcgacgctct ccagcgtgct
     2401 gtagatatcg acacagtcga aaatcttaaa gctggtttta ttcacctccg ggcataagcg
     2461 cgtggcgcgc cctttcatct gctcgtacag aatacggctg cgtaccttgc gcaggaacac
     2521 gatattacag atcgacggaa tatcgacgcc ggttgtcagc aggtcgacgg ttaccacgat
     2581 attgggcagc cgctctttat tgaagcgggt gatcatggtc tgcactttgc gcgcgtcttt
     2641 atcggcatca ccggtgatct tgatgatcgc gtcgtgctcc agttgcggat actttttctt
     2701 gaacgcggta cgcaactctt ccaccaccat atcggcatgg gcattggtaa cgcagaagac
     2761 cagcgttttt tgcgacccgg tcgggtcaag gtagttggtg agctcgttac agacggcgcg
     2821 gttaaacgcc gggatcacca ggccacggtt aaagtcggcg acctcaaaat cctgatcgtc
     2881 ttccagggta tcgttgatca cttctccctg cgggctgatg cgctctacct gttcgccctt
     2941 ggagagataa accccctcct gggcgttgcg ggtgatgatc tgaatcggcg gatcctggtc
     3001 gatcagaaaa ccgtcgataa ccgcggtacg gtaggtataa cggtaaaccg gctcgccgaa
     3061 aatctgcact gtatgcagcg ccggggtggc ggtgagggcg atttttaccg catcgaagtg
     3121 atcgagaatg cgacggtagg cagaaacgta atccagctgg ctgcgaaact gcagttcgcc
     3181 ttcggtctgc tctttatcga gaatatagcc gcgatgcgct tcatcgacga cgatacagtc
     3241 gtaacgggcc accggcatcg gttcatctga ttgcagggtg cgtttcacca gcgactgtac
     3301 ggtggcaacg tgaattttgg tgctgtcttc ggggaattta tccgtcagcc ctttaatgtc
     3361 gaaaatgctg ttgaaagtgt cgccgttaat acgcgtatct tcaaacgccc ccagcgcctg
     3421 ctcgccaaga gaacggcgat cgacaaggaa gagaatgcgt ttaaaacgct gggactggat
     3481 caggcggaac atcatggcaa ttgcggtgcg ggttttaccg gtaccggtcg ccatcgccag
     3541 caggatctct tgttgaccct tgacgattgc cttttcaacc gcgcggacgg catcttcctg
     3601 ataataacgc aggcctagct cgctcatgcc agggttatcg gcaaaccatt gattctgttt
     3661 ttgcggttcg ctgccgagca tttccagcag ctcttccggg cgatgccact cgggtaaggc
     3721 tttcgacata ttgcgggtat cacgcacgtc gcgataccag atgccacttt tagtcttcat
     3781 tgctgcgcgg tattcgcgcc cgttggtgga gtagcagaag gggattttaa accgttgttg
     3841 gccgctggtg tcctgccagc tagtttcata ctccggcaca gcttcatgca cttcatccgg
     3901 tgagtaatgc tcaagcaagg tttcccgcag gaagccatta tcgnnnnnnn nnnacaaata
     3961 ccggcaccgg gaagtcataa tctttggtga ccagacggta gtaccagaca gccaggcgga
     4021 acccgagtcg caggcacatc tgggcatcgt cgagatcgtt atggtattcg tgcaccgcct
     4081 ggttaccaat gcggcgtaat ttgtggaata cagagagaat gttgtcatca acgaaggcga
     4141 ttttgccgag ttcgcgcagg agatcgtgtt gattctcaca aggggggatg ttgagtaaca
     4201 taccaagatg tttcgctgta gcttcgccaa acatacgcat tttaatcagc gtcgtgttgg
     4261 gatcatccgg gtagttattt tccgccgcac aggcgatggc ataagtgaag tcgttgacgc
     4321 ccttcaggaa ttcgaagttg gatttattca tcattgttat taatccattg ctgtgtgggc
     4381 ctgtccaaat atttaaggcc cataacgccc tatcttagct ttctgtacct ttccgggcaa
     4441 tgaccacggt cacagcaact gactcatttc taacgtgttc gcctattttg gtagtgttat
     4501 agtagccgaa aaacatctac ctgattctgc aaggatgtac tatgacggtt ccaacctatg
     4561 acaaatttat tgaacctgtt ctgcgttatc tggcgacaaa accggaaggt gcagccgcgc
     4621 gtgatgttca tgaggctgcc gcggatgcat taggactgga tgacagccag cgagcgaaag
     4681 tcattaccag cggacaactt gtttataaaa atcgtgcagg ctgggcgcat gaccgtttaa
     4741 aacgtgccgg gttgtcgcaa agtttgtcgc gtggcaaatg gtgcctgact cctgcgggtt
     4801 ttgactgggt tgcgtctcat ccccagccaa tgacggagca ggagacgaac catctggcct
     4861 tcgattttgt gaatgtcaaa cttaagtcac ggccggatgc cgtcgattta gatccgaaag
     4921 ccgactctcc cgatcatgaa gaacttgcaa agagcagccc ggacgatcgg ttagatcagg
     4981 cgctaaaaga gcttcgtgat gcggtggctg atgaggttct ggaaaactta ttgcaggttt
     5041 ctccttcgcg ctttgnnnnn nnnnnttgtt ctggatgttt tgcatcgcct ggggtatggc
     5101 ggccaccgtg atgatttgca gcgtgttggc ggtactggag atggtggcat cgatggtgtg
     5161 atatcgcttg ataaacttgg cctggagaaa gtttatgttc aggcaaaacg ttggcagaat
     5221 actgtaggca ggccagaatt acaggcattt tacggcgcac tggctgggca aaaagcgaaa
     5281 cgtggggtgt ttattaccac ttctggattt acttctcagg cgcgtgactt tgcccaatcc
     5341 gtcgagggta tggtgttggt tgatggggaa cgcctggtgc acttaatgat cgaaaacgat
     5401 gtaggggttt cttcacgttt gttgaaggtg ccgaaactgg atatggacta ttttgagtga
     5461 aatatcaggc cggatgcggc tgcgccttat ccggcccata atcccttact tcctcaaccc
     5521 cgcaaacgcc gcccgaatct catcctccgg cagctgaatc ccgataaaca ccatcgtgct
     5581 atgcggtgtt tcatcgcccc acggcctgtc ccagtcagcg ctgtagaggc gctggacgcc
     5641 ctggaacagc aggcggttag gttcgccgtc aatccacagc atccctttgt aacgcagcag
     5701 tttatctgcc gattccagca gcaggttttc catcacgcgg gaaacttcgc tgatatctac
     5761 cgggtaatcc agttccacca caannnnnnn nnngtttatc cgcgataaag tggaaacgcg
     5821 gtttggtgct gacgacgttt tcttccagca taaaaccgtt ggtgttgaac aacaggccca
     5881 ggtcgatgtc gccgtgggtg acggtgtaga ccggtgcgcg ggcgttgatg cgtgccaggc
     5941 gttcgcgcag tttttctgct tcacctgcga cgtcggtttt agtcagcaga atacggtcgg
     6001 cgtagccaac
//
LOCUS       DAWDRC010000196         5981 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6047_length_5981_cov_2.138542, whole genome shotgun sequence.
ACCESSION   DAWDRC010000196 DAWDRC010000000
VERSION     DAWDRC010000196.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6047_length_5981_cov_2.138542"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2158..2167
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3479..3492
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1442 a         1513 c         1499 g         1503 t
ORIGIN      
        1 ccagcgcatg catggcgtca aagatcactt tgtcgatatc cgcccccgag gccgcgctga
       61 ccgttgctgc cagtgtacct tcgaccaacg gcgcagcaca caaacgtact tttgcggcga
      121 tctcgggagc cagcaattcc agcgcagttt cagcactcaa taatgcgcta cccatatcca
      181 tcatgaccag cacatggtcg gcatcggcaa cagattcgat ggcctccatc actttgacgg
      241 catcggtacc aatgggatta tgtggatcgt caattcccgc ggcaatggcg attttacaac
      301 tatcactcat taacatctga cgggctaatt caccgacacc ttctcccagt cggctgctat
      361 gtgaaactat gaccaggttt accatcacca attccttact cttttgcggc taacgccaac
      421 atttgcatca taaacatcac cgaggtcgcg ccgggatcct ggtgaccaat actgcgttca
      481 ccgagataac tggcgcggcc tttgcgggct tgcatcgtaa tcgtactttg tgcagcggat
      541 tcggcgatgc tactggcagc gtcgagcgcc gccggaacag ggagattttg ctcgcaggac
      601 agacgtaacg attccaccac cggcacccac acatcacaca tggttttatc gccaggttcg
      661 gctttcccgc gactgattac gccgtccgcg ccatcgcgga acatctgata aagctcttcc
      721 agcgtcaggc tttgccgtgc ctgggttgtc tgtgcggcgc ggataaagaa ggtaccgaac
      781 agcggaccac tggcacctcc gacgctggaa agtagcgtca tgccggtatt cttgagaatg
      841 aaaccgatat ctttatctgc gatagcaggg agtttttcca ccactttgcc aaagcctcgg
      901 ttcatattta gcccgtggtc agcgtcgcca atctcgcgat ccagtccagt aagatactcg
      961 ctctcggtgc tgaaaatatc gccacaacga gtgagccagt taacaatttg agttctgctc
     1021 agtgacattg ctttctcctt atttacccca gttaagggcc ggggtgtgga caggggcgtc
     1081 ccagagtgcc agcgtttcgt catcaacttt cagtaaggtg attgagaaac cggtcatatc
     1141 cagtgaggtg cagtacgcgc caattaaatt acgttcgata gtcaatcccg cttgctggca
     1201 acgtgtggtc aggcggttat agacgccgta cagctcagaa agcggagttg cgccaagatt
     1261 gttaaccagc gcaatcaccc gatcgccaga ctggagcggt tgtttggttt gtggttcttc
     1321 ctgccagctg ccttgttgat aatcccagaa acgcaaagtg cgatggtatg agccattttc
     1381 cagcagggtg tcgaacattt catcgacggt ttgatcaagg gaagagaagg ggcggcggtc
     1441 aatacctggc tcaccatgaa tgccgacgcc aaactccatc tcattatccg ccagggtaaa
     1501 agaaggtttg cccgcggcag gaacggtaca ggcaccgaga gcgataccta ttgaatggcc
     1561 ttgattattc aacttacgcc ccagttccgc acaggcgtcc agtgagtcgc cacgctccgc
     1621 cgctgcacct acgagttttt caattaatac ggtgttggca acgccgcgtc gcccggcggt
     1681 ataaagactg tcttttactg caacgtcgtc atcaatgacc acagtggtca cttttacgcc
     1741 gctatcgtgc agtaactcgg ttgctgtttc gaagttaaga atatcgccgg tgtaattttt
     1801 gataatcaac agtacacctt cgccgccatc aatttgcatg gcgcattcaa agattttatc
     1861 gggcgtcggt gaggtgaaaa tttcgcccgg acaggctccc gaaagcatcc cctgaccgat
     1921 atagccacag tgcatcggct cgtgtccgct gccgccaccc gacagcaggg cgactttccc
     1981 tgcaacgggg gcatcagctc gggtgacata caccggatcc tgatgcagtg tcagcgatgg
     2041 atgcgccttc gctagtcctg ccagttgttc gtccagtacg tcttgcacat cattgatcaa
     2101 ttttttcatt attttgctcc agcaattacg gtagggcatg gatgatgttc aacgacannn
     2161 nnnnnnntat gctttaatga atgttccata ttgaaacttt tacgtgtatt aatacttaaa
     2221 attgcgagcc ggaacacctt ttgtcataag ggatgcggga tatgagtggc gcttttaaca
     2281 acgatggtcg gggcatatct cccttaattg caacctcctg ggagagatgc aataagctga
     2341 tgaaacggga gacatggaac gtaccacacc aggcccaggg cgtgacattt gcttctattt
     2401 atcggcgtaa gaaagcgatg ctgacgctcg ggcaggctgc gctggaagat gcctgggaat
     2461 atatggcacc gcgagagtgt gcgttgttta tcctcgatga aaccgcctgt attctcagcc
     2521 gtaatggcga tccgcaaacc ttgcagcaac taagtacact gggattcaat gacggcacgt
     2581 attgcgccga gggaattatt ggtacttgtg cgctatcgtt agcggctatc tctggtcagg
     2641 ccgtgaaaac gatggccgat caacatttca aacaggcact ctggaactgg gccttttgcg
     2701 caacgccgtt gtttgacagc aagggccgat tgacgggaac aatagcgctg gcgtgtccgg
     2761 ttgagcaaac taccgcagct gatttgccgt tgacgttggc aatcgcacgc gaggtcggaa
     2821 atttactgct gacggacagt ttgctcgctg aaactaaccg tcatttaaat caacttaatg
     2881 ccctgttaga aagtatggat gatggcgtga ttagctggga cgagcagggt aatttgcaat
     2941 ttatcaacgc ccaggcggcg cgagtcttgc gccttgacgc gacggcaagt cagggaaggg
     3001 caatcactga actcttaacg ttacccgccg tattgcaaca agcaataaaa cagtcacatc
     3061 cgctcaaaca cgtagaagcc acctttgaaa gtcagcatca gtttattgat gcggtgataa
     3121 cccttaaacc gataatagaa acgcagggaa ccagctttat tttattgctc catcctgtgg
     3181 aacagatgcg gcagttaatg accagtcaat taggaaaagt cagccatacc ttcgctcata
     3241 tgccacagga cgatccgcaa acccgccgct tgattcattt tggtcgccag gcggcacgca
     3301 gtagctttcc tgtcctgctt tgtggagaag agggcgtggg caaggcactg ctaagtcagg
     3361 caattcataa tgaaagcgag cgtgcagcgg ggccttatat cgccgtcaat tgtgagttat
     3421 atggtgatgc ggcgctggcg gaagaattta ttggtggcga tcgcacggat agtgaaaann
     3481 nnnnnnnnnn nngcgtttaa tacccattga tgtcaaagtg attgccacaa cgaccgcgga
     3541 cctcgcaatg ctggtggaac aaaatcgttt tagtcgccag ctgtattacg cgctgcatgc
     3601 atttgaaatt accatcccgc cactgcgtat gcggcgtggc agcattccgg cgctggtgaa
     3661 taacaaatta cgcagccttg aaaaatgctt ctctacgcgg ctgaaaattg atgacgatgc
     3721 cctggcaacg attttgaact ttacagcgtc atcgagaatc ttgctctgag tagtgataat
     3781 gggcgcattc gcgtcagtga tttgccggaa catctgttta ccgagcaggc gacagatgat
     3841 gtcagcgcca cccgcctttc caccagtctg tcatttgcgg aagttgaaaa agaggcaatt
     3901 attaacgcag cccaggtcac aggcggtcgc attcaggaaa tgtcggcttt acttgggatc
     3961 ggccgcacta cgctgtggcg gaaaatgaag caacatggca ttgatgcagg gcagtttaag
     4021 cgccgtggat agcgggagag tatgtacttt cccgctatga attgctaatt aatagcgacc
     4081 atgactgccg tgatttggtc caccatttga aggtggcatt ggcttacctg agggcgcagg
     4141 accacgaggg ccatcgctgt ggcggtgatg atggtgggga acacaagcac tcaacaggct
     4201 tgcaaagaca attgctaaca ggctgaaggt aaatgttttt ttcatgacaa actcttgaac
     4261 ggttaatgca ctgtgattgt tatcacataa aagctgcatt gttaatattg tcaggagtct
     4321 gattatgagt tttggtaact cagaattgaa agaagacgcg tagcaggcgt aaaaaagcca
     4381 ttacgtgcaa ctcacataat ggctcgttat taaccgccag ggtagtatta gcggaattag
     4441 acgttgaaca ggaagttcat cacatcgcca tctttaacga tgtaatcttt gccttctgca
     4501 cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac
     4561 gagatggttt gtgcacggat aaagcctttt tcaaaatcag tatggatttt gcccgctgcc
     4621 tgcggcgcgg ttgcaccaac cggaatggtc catgcacgca cttctttcac cccagcggtg
     4681 aagtaagttt gcaggttcag cagcttataa ccggcacgga tcacacggtt caggcccggc
     4741 tcttccagcc caagctcctg cataaactcg tcacgctctt cgtcgtccag ttcggcaatg
     4801 tctgcttcaa cagcagcaca aaccggaact acaacagaac cttctttcgc tgcgatttca
     4861 cgcacctggt caagatacgg gttgttttca aaaccgtctt cgttgacgtt ggcgatgtac
     4921 attgttggtt ttagcgtcag gaagctcagg taacgaacag ccgctttctc ttcagcgctt
     4981 aaatccagcg cacgcagcat acctgcgttt tccagctggg gcaggcattt ttccagtacc
     5041 gccagctcgg ctttcgcgtc tttatcgcca cctttggctt tcttctgtac gcgatgaatc
     5101 gcacgttcac aggtgtcgag atccgccagc gccagttcgg tgttgataac ttcaatatca
     5161 tccgccgggt taactttgcc ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca
     5221 acgtgaccga tcgcttcggt ttcacggatg ttggtcagga actggttacc cagaccttcg
     5281 cctttcgatg cgccttttac cagaccggcg atatcgacaa attccatggt cgtgggaagt
     5341 gtacgctgcg gttttacgat ttcagccagt tgatccaggc gagggtcagg cattggtacg
     5401 acgcccgtgt tcggctcgat ggtgcagaat ggaaagttgg ccgcttcaat accggctttg
     5461 gtcagcgcgt tgaacagggt agatttcccg acgttgggca aaccgacgat accgcatttg
     5521 aatcccatga tttaaatcac cttaatgtct taataatcaa cctgttattt ctaacagatt
     5581 gcagaaatgg aaataacttt gcctattata cacggcactc ggcaaaaatg ccgcagacaa
     5641 cgacttattg cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag ccatctgtaa
     5701 accacatttc agtacaacgc gccgcttcgt cgatggcttc atcaattaac ttctgttcac
     5761 taacaggcgg tttgcctaac acaaaaccga caactttatt tttatcgccc ggatgaccga
     5821 ttccgatgcg taaacggtga aagttagggt tattacccaa tttactgatg atgtctttca
     5881 gtccattgtg accaccatga ccgccaccca acttaaattt ggcgacgcca ggaggcagat
     5941 ccagttcgtc gtgggccacc aaattttcgt ccggattaac g
//
LOCUS       DAWDRC010000197         5949 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6088_length_5949_cov_1.987784, whole genome shotgun sequence.
ACCESSION   DAWDRC010000197 DAWDRC010000000
VERSION     DAWDRC010000197.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5949)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6088_length_5949_cov_1.987784"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    443..452
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3518..3527
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1334 a         1521 c         1563 g         1511 t
ORIGIN      
        1 cagcccggat gatatcgcca atacgaaact ggtcgttatg ttcggaaata acccggcaga
       61 aacacggatg agcggcggtg gtgtcactta ctacgtcgag caagcccgcg aacgttcaaa
      121 cgcacgcatg atcgtcatcg atccacgtta taacgacaca gctgccgggc gtgaagatga
      181 atggctgccc attcgccctg gcaccgatgg cgcactggcc tgtgcaattg cctgggtact
      241 gattactgaa aacatggtcg atcagccatt tctcgacaaa tattgtgttg gttacgatga
      301 aaaaacgctg ccagctaacg ctccacgtaa cgcgcattat aaagcctata ttctgggcga
      361 agggcctgac ggcatagcta aaacgccgga atgggcagca aaaatcacca gcatcccggc
      421 agaaaaaatt atccagttgg cannnnnnnn nnaaaaccgg cttatatttg tcaggggtgg
      481 gggccacaac gacattccaa cggcgagcaa acgtcccgcg ctattgccat gctttccgtt
      541 ctcaccggta acgtcggcat aaacggcggc aactcaggtg tacgcgaagg tagctgggat
      601 ctgggggtag aatggttccc gatgctcgag aatcctgtta aaacgcagat ttccgtcttt
      661 acatggacag atgccatcga ccatggtacg gaaatgaccg cgccccgtga tggtgttcgt
      721 ggaaaagaaa aactggatgt ccccatcaag tttttatggt gctacgccag taacacattg
      781 atcaatcaac atggcgacat caatcacacc catgaggtgc ttcaggatga cagcaagtgc
      841 gagatgattg ttggcattga ccacttcatg actgcctcgg ctaagtattg cgatatcctg
      901 ttacccgacc tgatgccaac agagcaagaa gacctcatct ctcatgagtc tgccgggaat
      961 atgggctatg tgatcctcgc ccaacccgca acctcagcaa aatttgaacg caaacccatc
     1021 tactggatgc tgagtgaagt cgccaaacgc ttaggaccag acgtttatca aacctttact
     1081 gaaggtcgca gtcagcatga atggatcaaa tatctccatg cgaaaacgaa ggaacgtaac
     1141 cctgagatgc ccgactacga ggagatgaaa acgaccggga tctttaagaa aaaatgcccg
     1201 gaagaacact acgtcgcttt ccgcgcattc cgtgtagatc cacaggcaaa cccattgaaa
     1261 acaccttcgg gaaaaatcga aatttattct gaacgactgg cgaagattgc ggatacctgg
     1321 gaattgaaaa aagatgaaat tattcatcct cttccagctt agacgccagg ttttgatggc
     1381 tgggacgatc ccctgcggaa aacttatcca ctgcagttaa cgggcttcca ctacaaagcg
     1441 cgtacccact ccagttacgg caatattgat gtattacagc aggcctgccc acaagaggtg
     1501 tggatcaacc ccattgatgc tcaggcacgc ggtatccgtc atggcgatac cgtgcgggta
     1561 tttaacaata atggagaaat gctgattgcc gcaaaagtga ctccgcgtat tctgcctggc
     1621 gttaccgcca tcgggcaagg tgcttggctt aaagcggata tgtttggtga ccgggtcgat
     1681 cacggcggca gtatcaatat tctgacctct caccgccctt caccgctggc aaagggaaac
     1741 ccgtcgcaca gcaatcttgt ccagatcgaa aaggtttaag gagtagccca tgaccacaca
     1801 atatggattt tttatcgatt ccagccgctg tactggctgt aaaacctgcg aactggcgtg
     1861 caaagatttt aaagatcttg gcccggaagt cagtttccgc cgtatttatg aatatgctgg
     1921 cggcgactgg caggaagata atggcgtctg gcaccagaac gtgttcgctt attatctctc
     1981 catctcctgt aaccattgcg atgaccccgc atgtacaaaa gtctgcccca gcggtgcaat
     2041 gcataagcgt gaagatggtt tcgtggtggt tgatgaagat gtttgtatcg gctgccgcta
     2101 ctgccacatg gcttgtccgt acggcgcgcc acagtacaat gctgaaaaag ggcacatgac
     2161 gaagtgcgat ggttgttatt cgcgcgtcgc cgaggggaaa caacccatat gtgtcgaatc
     2221 ctgcccactg cgtgcacttg agttcggtcc aattgaagaa ttgcgccaga agcacggcac
     2281 tctggctgcc gtcgcgccac tgccgcgcgc gcatttcaca aaacccaata tcgttatcaa
     2341 acctaacgcc aacagccgcc cgaccggcga taccactggt tatctggcta atccggagga
     2401 agtgtaaaat gggaaatgga tggcatgaat ggccactggt tatctttacc gtattgggtc
     2461 aatgcgtagt aggcgcgctg attgttagcg gaattggctg gttcgccgcc aaaaataacg
     2521 cagaccgtca gcacattatt cgtggcatgt ttttcctctg gctgttgatg ggcatcgggt
     2581 ttattgcctc tgtcatgcac ctgggatcgc cactgcgggc gtttaactcg ctgaatagaa
     2641 tcggagcttc tggtctgagt aacgagattg ctgccggttc catatttttt gccgttggcg
     2701 gattatggtg gctggtggcg gtcatcggta aaatgccgca agcgttgggg aaactctggt
     2761 tacttgtcag catggcgctc ggcatcattt tcgtctggat gatgacctgc gtgtatcaaa
     2821 tcgacactgt gccaacctgg cataacggtt acactacgct ggctttcttc ctgacggtat
     2881 tgttgagcgg tccaatactt gctgccacaa ttctacgtgc agcccgcgtt acatttaata
     2941 ccacaccatt tgccatcatt agcattctgg cgctgatcgc ttgtgccggg ttgattgtgc
     3001 tgcaaggttt gtctctggcg tctattcact catccgtaca acaagccagc gcactggtac
     3061 cagattacgc ctccttacag gtctggcgtg tggtattgtt atgcgcaggt ctaggatgct
     3121 ggctttgtcc gctcattcgt cgccgtgaac ctcacgttgc cggacttgtt ctggggctaa
     3181 tcctcattct tggcggtgag atgatcgggc gtgtgctctt ttatggcctg cacatgacgg
     3241 taggaatggc tattgcaggt taacaaattg gtgcggggca acccgcacat ttcaggatgt
     3301 taggaatgac ccatttttca cagcaagata atttttctgt tgcggcgcgc gtgttgggcg
     3361 cgctgtttta ttacgctcca gagagtgcag aagccgcacc tctggttgcg gtactcaccc
     3421 gtgacggctg ggaaactcag tggcctctac cagaagcgtc attagcgccg ctggtgacgg
     3481 cttttcagac acagagtgaa gagactcacg cccaggcnnn nnnnnnngcc cgtgggcact
     3541 gccgtctccg ccgtggggat cggtctggct ggatcgcgaa tctgtgctgt ttggcgattc
     3601 aacattggca cttcgtcagt ggatgcgcga gaaaggcatt cagttcgaaa tgaagcaaaa
     3661 cgaaccggaa gatcattttg gttcactgct gttgatggct gcgtggttgg cggagaatgg
     3721 tcgccagaca gaatgcgaag aattgctggc atggcacctt tttccgtggt caacacgttt
     3781 tcttgacgtt tttatcgaaa aagcagaaca ccctttctac cgtgcactgg gtgaactcgc
     3841 tcgcttaacg ctggcgcaat ggcaatcaca actgttgatt cctgtcgcgg ttaaaccgct
     3901 gtttcgatag gatcaccgta ttattgccgg atggtgacga ttacacgcaa ttcggcaatt
     3961 aataggttac agtgtcacgt tttttttatc tcttaaagca cgcactgctt ttgcggctgg
     4021 cctcttttgc cgcaaaatag tcgcccgtgt ttcattgccc atttctgctc atgcatcatc
     4081 tacacatcta tcctgatctg cgcactatgt tccgccgtct gcttatcgca acagtcgtcg
     4141 gtattctcgc ggcctttgcc gttgccgggt ttcgtcatgc gatgctgcta ctggagtggt
     4201 tgttcctcaa taatgactcg ggcagtctgg tcaatgcagc gacaaatctt tccccctggc
     4261 gacggctgct aactccggcg ctcggcggac tggcggcggg tttgttgctg atgggctgga
     4321 agaaatttac ccagcaacgc cctcatgcgc cgaccgatta catggaagcg ttgcaaaccg
     4381 atgggcagtt tgattacgca gcaagcctgg ttaaatccct tgcctctctg ctggtagtaa
     4441 ccagcggcag tgcaattggt cgcgaaggtg cgatgattct tttagctgcc cttgccgcct
     4501 cctgttttgc gcaacgtttt acgccacgcc aggagtggaa attatggatc gcctgtggag
     4561 ctgcggcggg gatggctgcg gcctatcgcg ccccgcttgc tggcagttta tttatagccg
     4621 aagtgctgtt tggcactatg atgttggcct ctctcggccc ggtgattatt tccgccgtcg
     4681 tggcgttgct ggttagcaat ctgattaatc atagcgacgc gttactctac agtgtacaac
     4741 tctcagtgac ggttcaggct cgtgactatg cgctgattat cagtacaggt gtgctggcag
     4801 gtctgtgcgg accactgttg ttaacgttaa tgaacgcctg tcatcgtgga tttgtgagtc
     4861 tcaagcttgc gccgccctgg caactggcac tgggcgggtt gatcgtgggt ctgctttccc
     4921 tgttcacacc tgcagtgtgg ggcaacggct atagcaccgt acaatccttt ttaaccgctc
     4981 cgccactgtt aatgatcatt gccgggatct tcctctgtaa actgtgtgcc gtgctggcga
     5041 gtagtggttc tggagcgccc ggtggtgtct ttacaccgac gctatttatc ggtcttgcca
     5101 ttggcatgtt gtatggtcgt agcctgggat tatggttacc tgatggcgaa gaaattacgc
     5161 ttttactcgg attgacaggg atggcgacac tgttggcggc aacaacgcac gcgccgatta
     5221 tgtcgacgtt gatgatatgt gaaatgaccg gggagtatca gctactcccc ggtttattga
     5281 ttgcctgcgt aattgcgtcg gtaatttcgc ggacgttaca ccgtgactct atctaccgcc
     5341 agcacactgc gaagcatagc taagcggatg tattgcccca gttcacgctg ttcggcgcgc
     5401 ggcagataag gcagttcacc aatgagcggt gccggaagtt ttttaccgag cacatcaatg
     5461 atttccgcat aatgcgccag tcctgggttg attcggttag ccacccagcc aatgagcggc
     5521 agcccgtcgt tggcaatcgc ctgagctgtt agcagtgcat ggttaatgca accttcctga
     5581 ataccgacaa ccatcaacac cggcagttgt tcctgcacca cccattcaga gagcggacgc
     5641 aaatcattca tcagactgcg ccagccgcca gtcccttcta ccacgacatg atcgacttta
     5701 tcggtcaggt ttgccaggcc gtttgaaagg agggtgtaat tgattgggca actgtgcgcc
     5761 acgctacttt cttcttcgct taacgcgata ggattaactg cttcataagg cagttcgatg
     5821 gttgaaacac tctgcaacac cagggcatct ttattacgca gcccttcggg tgtctctttg
     5881 ctccccttcg ctacgggttt atatcccgca accgtttttc cctgggaggc taacgcttgt
     5941 agcaatgcg
//
LOCUS       DAWDRC010000198         5945 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6096_length_5945_cov_1.898981, whole genome shotgun sequence.
ACCESSION   DAWDRC010000198 DAWDRC010000000
VERSION     DAWDRC010000198.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6096_length_5945_cov_1.898981"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2645..2654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3451..3460
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4309..4318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5269..5278
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1440 a         1401 c         1416 g         1648 t
ORIGIN      
        1 tgatacatca gcccctggtg ctcaccaatt tcatcgccat cgatggtaat gattttgccc
       61 ggttgcgccg ggagataacg gcccaggaac tcgcggaatt tacgctcgcc gataaagcag
      121 ataccggtag agtctttttt cttcgcggtg accagaccga gatcttcagc aatcttacgc
      181 acctgcggtt tttccagttc gccgaccggg aacaggcttt gcgcaatttg ctcatggctg
      241 agcgtataaa ggaagtagct ctggtcttta ttgctgtcca ggccacgcag caggcggctc
      301 ttgccatcga catctgcccg acgtacgtaa tgaccggtag cgatataatc ggcacctaaa
      361 tcttcggcgg caaattcgag gaaagcttta aatttgatct ctttgttgca cagaatatcc
      421 ggatttggcg tgcgaccggc tttatattcg gcaaggaaca gttcgaagac gttgtcccag
      481 tactcggcag caaagttaac ggtgtgcagt tcaatgccga gcttgtcgca gacagcctgg
      541 gcatcagcca gatccgctgc cgctgtgcaa tattcctcac cgtcgtcttc ttcccagttc
      601 ttcataaaca ggccttcgac ctgatatccc tgttgttgca acagccaggc agaaacggag
      661 gaatcgacac cgccggacat gccgacgatt actttttttg cggtttcaga cattggatca
      721 ctcacgacat tgaacttcaa ggcggcgtat tctatcacgc agccttttag atgacaccct
      781 ttgtaaaagg ccagttaaaa tcgccaatca tctccagcgg atagcgttgc ccgctttgat
      841 aacaacgaat actttccgcc accagcggcg aacgaagatt tgacgcctgt aaaatttctt
      901 cggcgctgac ccaacggcag cagtcgatat cgctgtcatg aggctgcgtc gggcatattt
      961 gctcaagctc aatggcaaag aggaaacgca aaaacggcgt tttatccggc gcaatccact
     1021 gatgcatacg aataaagtgt tgcggctgcg cgctaatgcc ggtttcttcc cacagctcac
     1081 gggcggcggc ttccactaag gtttcatcgg cttccaggtg cccggcaggt tggttccata
     1141 acgctttacc attaatcgtc tcttcaacga ctaaaaattt gccttctgcg tgcaccacgc
     1201 aggcaacggt aacgtgcggt ttaaacattt tgcatcctta atctgtcgcg tctcgccatt
     1261 cgccattggc aagattatcc aaagagtaat cccccatcgc atagcgaatc agtcgcagcg
     1321 tggggaaacc aacatgggcg gtcatgcggc gcacctggcg attacgtccc tcatataagg
     1381 tgatcttcag ccagctggtg ggaatacttt tgcgctcacg aattggtgga ttccgcggcc
     1441 ataaccacgc gggttcttca accagctccg cgcctgcggg cagggtaggg ccatcattta
     1501 aggttacgcc attgcgcaag gcttcaagtg cgtcttgtgt gggaatacct tccacctgca
     1561 cataatagat ttttccggtg cgtttacccg gctgggttaa acgcgcctgc aacgcaccgt
     1621 tattggtcag caccagtaac ccttcgctgt cgcggtcgag acgacctgcc gcatacaccc
     1681 cctgaaccgg gatgaattct tttaatgttt tgcgtccggc ttcatcggtg aactgcggaa
     1741 gaacatcgta gggtttattg aacaggatca cacgcgtggg ctggttttca ggtttacgcc
     1801 tggtagaacg ttgcgagctg aatcgcttaa cctggtgatt tctaaaagaa gttttttgca
     1861 tggtattttc agagattatg aattgccgca ttatagccta ataacgcgca tctttcatga
     1921 cggcaaacaa tagggtagta ttgacaagcc aattacaaat cattaacaaa aaattgctct
     1981 aaagcatccg tatcgcagga cgcaaacgca tatgcaacgt ggtggtagac gagcaaacca
     2041 gtagcgctcg aaggagaggt gaatggaaag taaagtagtt gttccggcac aaggcaagaa
     2101 gatcaccctg caaaacggca aactcaacgt tcctgaaaat ccgattatcc cttacattga
     2161 aggtgatgga atcggtgtag atgtaacccc agccatgctg aaagtggtcg acgctgcagt
     2221 tgagaaagcc tataaaggcg agcgtaaaat ctcctggatg gaaatttaca ccggtgaaaa
     2281 atccacacag gtttatggtc aggacgtctg gttgcctgct gaaacccttg atctgattcg
     2341 tgaatatcgc gttgccatta aaggcccgct gaccactccg gttggtggcg gtattcgctc
     2401 tctgaacgtt gccctgcgcc aggaactgga tctctacatc tgcctgcgtc cggtacgtta
     2461 ctatcagggc actccaagcc cggttaaaca ccctgaactg accgatatgg ttatcttccg
     2521 tgaaaactcg gaagacattt atgcgggtat cgaatggaaa gcagactctg ccgacgctga
     2581 gaaagtgatt aaattcctgc gtgaagagat gggcgtgaag aaaattcgct tcccggaaca
     2641 ttgcnnnnnn nnnncggtat cggtattaag ccgtgttcgg aagaaggcac caaacgtctg
     2701 gttcgtgcag cgatcgaata cgcaattgct aacgatcgtg actctgtgac cctggtgcac
     2761 aaaggcaaca tcatgaagtt caccgaaggc gcgtttaaag actggggcta ccaactggcg
     2821 cgtgaagaat ttggcggtga actgatcgac ggcggcccgt ggctgaaagt taaaaacccg
     2881 aataccggca aagagatcgt cattaaagac gtgattgctg atgcattcct gcaacagatc
     2941 ctgctgcgtc cggctgaata tgatgttatc gcctgtatga acctgaacgg tgactacatt
     3001 tctgacgccc tggcagcgca ggttggtggt atcggtatcg cccctggcgc aaacatcggt
     3061 gacgaatgcg ccctgtttga agccacccac ggtactgcgc cgaaatatgc cggacaggac
     3121 aaagtaaacc caggctctat tattctctcc gctgagatga tgctgcgcca tatgggctgg
     3181 actgaagctg ctgacctgat tgttaaaggt atggaaggcg cgatcaacgc caagactgta
     3241 acctatgact tcgaacgtct gatggaaggc gctaaactgc tgaaatgttc agagtttggt
     3301 gacgcgatca tcaagaatat gtaatcacta catgtgttaa atattgaaac gggcgtataa
     3361 cacgcccgtt gttttatgat gatgtaaaat cttccccaaa actttcccca aaacccttcc
     3421 ccaaaactgg ctattttcta tgctgttttg nnnnnnnnnn ctacgataat ccagtcttta
     3481 ccacgatcat cattgtatcg gtcggtcatt tttctggatt tatgacctaa caacttttgc
     3541 gtatccagcc cctgttcccg atataaccgt tctgacagag atcgctgctc atgaaatgtg
     3601 ggcgcagttc ctggctccca ttttatgcca catttttccc tggccttttt aaaagccgtt
     3661 gtaagagtat ttgcagaaac ctggtctcct ctgtttgctt gagaggtagt gtgacggtaa
     3721 tggaccagat atttactaac aacagcatcc ctgcactgag atataacttc acgaagggta
     3781 atattcagag catcgcattt caggctaagc ggaatagcaa gttttgaacc ggttttttcc
     3841 tgagtaatgt gcaacatgtc gtcccatata tcagagaatt tcaaattgca gatatcgcct
     3901 aaacgttgtc cagtgacaag agcaagtagc atcccgcatt ttaaataggg ctgccgtctg
     3961 cttacgctgt caaatattgc ctgccattcg gacagtgata atctttggcg gtttactcga
     4021 tttcgcggtt gttttgttgc ctgcgctggg ttaaatcctg gcggaacatg tcctgcgtgt
     4081 tgtgcttctt tgaagacgtc gatcaacacc attctcacga cttgcgccat cctgttatga
     4141 ccttcagcct ttacagcatc aattatttcg gcaatatcaa gtgcggtaat atccttgagg
     4201 tgttgcattc cacaatgctc acggaagaga cgaatgggtt tgcctttttg ccgataggag
     4261 ttgggtctta gttcattatg ttgcagcctg tcctcctgga tcgtagtann nnnnnnnntc
     4321 ttggatagaa atatatttat caagccattc tgtcaccgta atgtctgagc gcctgccttt
     4381 cattctttcc agacgctcat tgacgcttaa tatttgtcgg gtacgttgtt cagcaataat
     4441 ggtatttgct tcagtagcaa cctgttttgc ttcattctca tcagttccta aactatgaaa
     4501 acgaccggat agtggatgtt tgtattgcca atataccttt ccggttcgct tatctaattt
     4561 gcaatataaa ttgggtatag agattttgtg agatcggggt ctagcagcca tcagcgatta
     4621 tccgttggag ttttgggttt gcgttgattg ggagttgcgg ttctgcaagc gttcctacaa
     4681 aacgggaatt tcggtcaatc atccagtagc gaccaacttt tatagcgggt ggggccatca
     4741 ttttcccttg cgcgtatttt ttcagaactc gctcacttgg tgctaagtcc ccaaattctt
     4801 ctttagccca gtcctgtaaa gtgattagtc gagacatttg tcctcctctt agctgctgag
     4861 ggagtttgtg accgatatat ctgacatgat attaagctta tggcaggtac atctcttgac
     4921 tggtcataga gataaattta atgctgagaa atgcagtatt gaatttatca atttttctat
     4981 ttcctgcgta tggcacgtaa cttcttaatg tgttctgccg tttcgatctc ttctgctatc
     5041 cgatctgcat cagctttatt cacaggttca aagtcatgat taaagcggaa catgctggcg
     5101 atacatgttc tgccttttcg gatgtagtga actttgttgt gggtagaacg caggattttg
     5161 cagggagtgc cgtggtgatc gacgtaccag gtgttaggaa aaatgattct gaacattttt
     5221 acacctcaat tggacgatgt taaaatttgc tgctttgagg ccatcacann nnnnnnnnct
     5281 cctcctggca acttgcacaa gtccgacaac cctgaacggc caggcgtctt cgttcatcta
     5341 tcggatcgcc acactcacaa caatgagtgg cagatacagc caggtagttc aggcggcgca
     5401 tttttattgc tgtgttgcgc tgtaattctt caatttctga tgctgaatca atgatgtctg
     5461 ccatctttca ttaatccctg aattgttggt taatacgctt gagggtgaat gcgaataata
     5521 aaaaaggagc ctgtagctcc atgatgattt tgtttttcat gttcaccgtt ccttaaagac
     5581 gccgtttaac atgccgatcg ccaggcttaa atgagtcggt gtgaatccca tcagcgttac
     5641 cgtttcgcgg tgcttcttta gtacgctacg gcaaatgtca ttgacgtttt tatccggaaa
     5701 ctgctgtctg gcttttttga tttcataatt agcctgacgg gcaatgctgc gaagggcgtt
     5761 attatgttct attgtcatat tggcctcaca cttcgaatgc cagttgaggg gtaaagacgt
     5821 cccgttcagc gttgtaatta agtgaactgg cactgttgaa tgattcaatg cgttccacaa
     5881 gaacttgcgt acgggtttct ttacttgcgg gagcatatgg cgaacctacc caggatttgt
     5941 cgatg
//
LOCUS       DAWDRC010000199         5915 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6133_length_5915_cov_2.106826, whole genome shotgun sequence.
ACCESSION   DAWDRC010000199 DAWDRC010000000
VERSION     DAWDRC010000199.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6133_length_5915_cov_2.106826"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1409 a         1388 c         1634 g         1484 t
ORIGIN      
        1 ttcagcaaaa gactggcagg aagctatcga tttctcgatg gtatcattgc tggataaaaa
       61 ctatatcagc gagaattaca ttcaggcaat taaaggttcc accatcagca atggtcctta
      121 ttatattctc gcaccaggcg tggcaatgcc tcatgcgcga ccggaatgtg gagcgcttaa
      181 aaccgggatg tctttgacgt tacttaaaca aggcgtttat tttccgggga atgacgaacc
      241 gattaaatta ctcatcggac tttctgctgc cgatgccgat tcgcacattg gtgctattca
      301 ggcgttaagt gaattactgt gcgaagaaga aatactcgaa caactcttaa ccgcatcatc
      361 agaaaaacaa ttagcggaca ttatcagccg cggataatga ctctctcctc tacaggataa
      421 tattatggaa aacaagtctg ctcgtgcaaa ggtccaggct tttgggggat ttttgactgc
      481 aatggtcatc cccaatatcg gtgcttttat tgcctgggga tttataacgg cattatttat
      541 tcccaccggt tggctgccga atgaacattt cgccaaaatt gttggtccga tgattaccta
      601 tttattgccc gtgatgattg gttctaccgg tggtcatctg gtcggcggta aacgcggcgc
      661 ggtcatgggc ggaattggta cgattggtgt gatcgtcggc gctgaaatcc cgatgttcct
      721 tggctcaatg attatggggc cgctcggtgg tctggtgatt aaatatatcg acaagtcgct
      781 ggaaaaacgc atacctgctg gctttgagat ggttatcaat aacttctcat taggtatcgc
      841 ggggatgctc ctttgtctgc tgggttttga agtgatcggc ccggcggtgt taattgctaa
      901 tactttcgtc aaagagtgta ttgaggcgct ggtacatgcg ggttatctac ctttgctgtc
      961 agtcatcaat gaaccggcga aagtgctttt ccttaataat gcgatcgatc agggcgtcta
     1021 ttatccgctg ggaatgcaac aggcttcagt taacggcaaa tccatcttct ttatggtggc
     1081 atccaatcca gggcctggct taggactgtt gctggcattc actctgtttg gtaaagggat
     1141 gagtaagcgc tctgcacctg gtgcgatgat tattcacttc ctcggtggga tccacgaact
     1201 gtatttcccg tatgtgctga tgaagccgct gaccattatt gccatgattg cgggcggtat
     1261 gtctggcacc tggatgttta acttactgga cggtggtctg gtggctggcc caagtccggg
     1321 gtctatcttt gcttacctgg cactgacgcc gaaaggctcg ttcctggcga caattgccgg
     1381 tgttacggta ggtaccctgg tgtcctttgc tatcacttcg ctgatactga agatggaaaa
     1441 aacggtggaa acggagagcg aagatgagtt tgctcagtca gccaatgcgg ttaaggcgat
     1501 gaaacaagag ggggcattct cgttaagccg ggttaagcgt atcgcctttg tttgcgatgc
     1561 ggggatgggc tccagcgcga tgggcgcaac caccttccgt aaacgtctgg aaaaagcggg
     1621 gctggcaatt gaagtaaaac attacgcgat agaaaacgtg cctgcggatg cggatatcgt
     1681 cgttactcac gccagtctgg aagggcgcgt gaaacgtgtg acggataaac cactgatatt
     1741 gattaataac tatattggcg atccaaaact cgacacttta tttaatcaat taaccgctga
     1801 acataaacac tgattacaga ggtaaaaatg aaaaccaaag ttgctgctat ttatggcaag
     1861 cgggatgtcc gtctgcgcga atttgagctg ccagaaatta ccgataatga actgttagtg
     1921 aatgtaattt ctgacagcgt ttgtttatcc acctggaaag cggcgttact cggtagtgaa
     1981 cataaacgcg tccccgacga tttagaaaat catccggtca ttaccgggca tgaatgtgct
     2041 ggggttattg tcgaagtggg taaaaatctc actggcaaat ataaaaaagg ccagcgtttt
     2101 gtattgcaac cggcgatggg gttaccaggc gggtattcag cgggctacag ctacgaatat
     2161 tttggcggta acgccaccta tatgattatt cccgaaatcg cgattaattt aggctgtgta
     2221 ttaccgtatc acggctctta ttttgctgcg gcctcgctgg cggaaccgat gtgctgcatt
     2281 attggcgctt atcatgccaa ttatcacacc acgcaatatg tttatgagca tcgtatgggc
     2341 gtcaaacctg gcggcaatat tgccctgctg gcgtgtgctg gcccgatggg cattggcgct
     2401 atcgattacg ccattaacgg cggcatacaa ccgtcgcggg tggtggtggt cgatatcgac
     2461 gacaaacgcc tgacgcaggt acaaaagctg ctgccggtgg aactggcggc cagtaaaggc
     2521 attgagctgg tgtatgtgaa tactaaaggg atgagcgatc cggtccagac gctgcgggcg
     2581 ttgacggatg atgccggatt tgatgacgta tttgtctatg cagcggtgcc ttcagtcgtc
     2641 gagatggctg atgaattact ggctgaagat ggctgcctga acttctttgc cggtccgacg
     2701 gataaaaact tcaaagtgcc gtttaatttc tacaacgtcc attacaacag tacgcacatt
     2761 gtcggcacgt ccggcggttc cacggacgac atgaaagagg cgattgccct tagcgccacg
     2821 gggcagttac agccgtcgtt tatggtgacc catatcggtg gcctggatgc ggtgccagaa
     2881 accgtgctca atctgccgga tatccctggc ggtaaaaaac tcatttataa cggcgtgacc
     2941 atgccgctga ccgccattgc cgattttgcc gaaaaaggca aaaccgatcc gctgtttaaa
     3001 gagctggcgc ggctggttga ggaaacgcac ggcatctgga atgaacaggc cgagaaatat
     3061 ctgctggcac aatttggcgt tgatattggg gaggccgcgc aatgatgtct ctggcgtggc
     3121 cattatttcg cgttacggaa caggcagcgt tggctgcctg gccgcaaacc ggctgtggcg
     3181 acaaaaataa aattgatggc cttgcggtca ccgcgatgcg ctaggcatta aacgacgttg
     3241 cttttcgtgg gcgagtggtt atcggcgagg gggaacgata tcctctttag cgctacgggc
     3301 gtgacggacg gcgagttggt gaacggtatc caacagacgg cgaatggggt gcggacgcag
     3361 acattactga tcggcggcgc ggaccaaacg tgtaatataa tagactccct gcattgatgg
     3421 tgatctatcg gaagaccaat ggcgacgctg acagaagatg atgtgcttga gcaactggat
     3481 gcacaggaca atttattttc atttatgaaa actgcgcatt ctattttgct ccaggggata
     3541 cgccagtttc tgccgtcgct gtttgtcgat aacgatgaag agatcgtcga atatgcagtg
     3601 aagccgttac tcgcccaaag cggcccgctt gacgatattg atgttgcgct gcgtttgatt
     3661 tatgcgctgg ggaaaatgga taaatggctg tacgccgata tcacgcattt ttcccagttt
     3721 tggcattacc tgaacgaaca ggatgaaacg cccggatttg ccgatgacat ggcctgggat
     3781 tttatcagca acgtcaatag cataatccgc aatgcgagtc tctatgacgc gttgaaagcg
     3841 atgaaattcg ccgatttcgc cgtctggtca gaggcgcgtt ttagcgggat ggtcaaaacg
     3901 gcgctgacgc tggcagtaac gaccacctta aaggaattaa caccgtgaaa atcgaactga
     3961 cggtgaatgg actaagggta caggcgcagt actctgatga tgaaattgaa aatgtacata
     4021 aaccgttgct gcgtatgctg gcggccttac agacagtaaa tccgcagcgg cgtacggtgg
     4081 tttttctttg cgctccgccg gggacgggta aatctaccct aaccaccttc tgggaatatc
     4141 tcgcacaaca agacccggaa ctgcccgcta ttcagacact gccgatggat ggttttcacc
     4201 attacaatag ctggctggat gcgcataaat tgcgcccctt caaaggcgca ccagagacat
     4261 tcaacgttgc gaaactggcg gaaaatctgt gccgggttgt ggaaggggat tgtacgtggc
     4321 cgcagtacga tcgacaaaag catgatcctg ttgaagatgc gttggacgtt accgctcctc
     4381 tcgtcatcgt cgaaggaaac tggttgttgc tggacgacga gaagtggtgc cagcttgcgc
     4441 aattttgtga tttctcgatc tttatcaaag ctcccgcatc tgcgttgcgg gagcgtctgg
     4501 tggggcgcaa gctggcggga gggttatcgc tggcagacgc agaagccttt tatgaccgta
     4561 ccgacgggcc gaatgttcgc cgggtgctgg aagagagtct gcctgcaaat ttgaccttaa
     4621 tgatgaccgc tacgggtgaa tatcgtctga tggattaacg ttcctgatta caccatcttc
     4681 cagtcgcacc acacgagaga aatgacgccg ggtaaatgcg gcgtcatgac tgattgctac
     4741 gacgctagtg cctcgctggc ggcacttctc cagccagctt tcaaacacgc tcaaccaatt
     4801 ttcgtcaaaa tcccgactcg gctcgtccag tagcaatagt ggcggagaga gtgcttccag
     4861 gcaggcgacg gcgaccatcc gtcgctgtgc actatgcaga tccaacggat gcgcgctggc
     4921 agtgtctgct aactgacagc attgcagggc agcattggta cgttgagtga tttcatctgc
     4981 cgggcatttt tgcagtttca aaccaaatgc gatctcgtca gcaacggtgc tgtgaaaaag
     5041 ctggttttcc gcttcctgaa acagtacgcc aatctttgcc gcgcgctggc ggtttttcag
     5101 gtttttcatc gcttgttgtt gcagcataac cgtaccggct gtgggcgtaa gaagacctgc
     5161 cattactcgt aacagcgtcg atttccctgc tccattatcg ccggtcagcg ccagccattc
     5221 accttgtttg agttgcagag aaatgtcgca aagacaatcc gttgccgcgc caggccagcg
     5281 ataagatatc tgatttaacg ttaacatagt ggctggagcg ctccgttttg caattgccac
     5341 gcttgctggc agtggttcag gaaaggtgtg tgatgacgtt caaaaagaat aatcagcgaa
     5401 ccacgttcaa gcgcccagtg ttgtaagcgc tgcaacagca tttcgcgggc ctgcggcgtc
     5461 aggcggctaa aggcttcatc aagaattaag agctttggct gcatcgcgat ggcgcaggcg
     5521 atgaccacgc gctgtgtctc tccaccggag agtgttgccg ggtgacgatg gcgcaacggc
     5581 tggcactcgg tcagggccag ggcggcgtca atccgcgcca taatctcttt ttcagcaaga
     5641 cacagatttt ctggcccaaa ggcaacttct tcttccacgc taaaggtaca gccggagagc
     5701 tgcaaatagg gcgattgctg gacgagctgg atggtggcgg attgttcatt aagcgggaga
     5761 cgcccaatgg gcgtaccgag aagcgtcccc gtgccggtta tctcttctgg tagaaagtcc
     5821 ggataccagc ccgccatcag ctgcgccagc gtacttttgc cactgccgtt gtcaccaaag
     5881 atggcgacca tccccggttt gacatagtga aagtc
//
LOCUS       DAWDRC010000200         5911 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6138_length_5911_cov_2.092384, whole genome shotgun sequence.
ACCESSION   DAWDRC010000200 DAWDRC010000000
VERSION     DAWDRC010000200.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6138_length_5911_cov_2.092384"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    617..626
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1115..1124
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1955..1964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2560..2569
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3402..3411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1335 a         1573 c         1707 g         1246 t
ORIGIN      
        1 atcgcgatgg gcggctctac caacaccgtt ctccacctgc tggcggcggc gcaggaagcg
       61 gaaatcgact tcaccatgag tgatatcgat aagctctccc gcaaagtacc gcagctgtgt
      121 aaagttgcgc cgagcaccca aaaataccat atggaagatg ttcatcgtgc tggtggtgtt
      181 atcggtattc tcggcgagct ggatcgtgcg gggttactga accgtgatgt gaaaaacgtg
      241 cttggcctga cgttgccgca aacgctggaa caatacgacg ttatgctgac ccaggatgac
      301 gcggttaaaa atatgttccg cgctggcccg gcgggcattc gtactacaca ggcattctcg
      361 caggattgcc gctgggatac gctggacgac gatcgctcca atggctgtat ccgctcgctg
      421 gaacacgcct acagcaaaga cggcggcctg gcggtgctct acggtaattt cgcagaaaac
      481 ggctgcatcg ttaaaaccgc aggcgtcgat gacagcatcc tcaaattcac tggcccggcg
      541 aaagtctacg aaagccagga cgacgcggta gaagcgattc tcggcggtaa agttgtcgcc
      601 ggagatgtgg gagtaannnn nnnnnngcta tgaaggcccg aaaggcggtc cggggatgca
      661 ggaaatgctc tacccaacca gtttcctgaa atcaatgggt ctcggtaaag cctgtgcgct
      721 gatcaccgac ggtcgtttct ctggcggcac ctctggtctt tccatcggcc acgtctcacc
      781 ggaagcggca agtggcggca gcattggcct gattgaagat ggtgacctga tcgctatcga
      841 cattccgaac cgtggcattc agttacaggt aagcgatgcc gaactggcgg cgcgtcgtga
      901 agcgcaggaa gctcgtggca acaaagcctg gacaccgaaa aatcgtgaac gtcaggtttc
      961 ctttgcgctg cgtgcctacg ccagcctggc aaccagcgcc gacaaaggcg cggtgcgtga
     1021 taaatcgaaa ctggggggtt aatgatggca gactcacaac ctctctccgg cgccccggaa
     1081 ggcgccgaat atttaagagc ggtgctgcgc gcgcnnnnnn nnnngtctac gaagcggcgc
     1141 aggtcacgcc gctacaaaaa atggaaaaac tctcgtcgcg tctcgataac gtgattctgg
     1201 tgaaacgcga agaccgtcag ccagtgcaca gctttaagct gcgcggcgca tacgccatga
     1261 tggcgggcct gacggaagaa cagaaagcgc acggcgtgat caccgcttct gcgggtaacc
     1321 acgcgcaggg cgtcgcgttt tcttccgcac ggttaggcgt gaaggcactg atcgtcatgc
     1381 caaccgccac cgccgatatc aaagtcgacg cggtgcgcgg cttcggcggc gaagtgcttc
     1441 ttcacggcgc gaactttgat gaagcaaaag cgaaagcgat cgaactatca cagcagcagg
     1501 gcttcacctg ggtgccgccg ttcgaccatc cgatggtgat cgccgggcaa ggcacgctgg
     1561 cgctggaact gctccagcag gacgcccatc tcgaccgcgt atttgtgcca gtcggcggcg
     1621 gcggtctggc ggcgggcgtg gcggtactga tcaaacaact gatgccgcaa atcaaagtga
     1681 ttgccgtgga agcggaagac tccgcctgcc tgaaagcggc gctgaatgca ggtcatccgg
     1741 ttgatctgcc acgcgtgggg ctgtttgccg aaggtgttgc ggtgaaacgc atcggcgacg
     1801 aaaccttccg tttatgccag gagtatctcg acgacatcat caccgtcgat agcgatgcca
     1861 tatgtgcggc gatgaaagac ctgttcgaag atgtgcgcgc ggtggcggaa ccctctggcg
     1921 cgctggcact ggcgggaatg aaaaaatata tcgcnnnnnn nnnncgcggt gagcggctgg
     1981 cgcatattct ttctggtgct aacgtgaact ttcacggcct gcgctacgtc tcggaacgtt
     2041 gcgaactggg cgaacagcgt gaagcgttgc tggcggtaac catcccggaa gagaagggca
     2101 gcttcctgaa gttctgccag ctgcttggcg ggcgctcggt caccgagttc aactaccgtt
     2161 ttgccgatgc caaaaacgcc tgcatttttg ttggtgtgcg cctgagccgt ggtctcgaag
     2221 agcgcaaaga aattttgcag atgctcaacg atggcggcta cagcgtagtg gatctctccg
     2281 acgacgaaat ggcgaagctg catgtgcgct atatggtcgg cgggcgtccg tcgcatccgt
     2341 tgcaggaacg cctctacagc ttcgaattcc cggaatcgcc gggcgcgctg ttgcgcttcc
     2401 tcaacacgct gggtacgcac tggaacattt ctttgttcca ctatcgcagc catggcaccg
     2461 actacgggcg cgtactggcg gcgttcgagc ttggcgatca tgaaccggat tttgaaaccc
     2521 ggctgaatga actgggctac gattgccacg acgaaaccan nnnnnnnnna ataacccggc
     2581 gttcaggttc tttttggcgg gttagtggtt cggcaaaatc ttccagaacg cctcaatcag
     2641 cggctcatgt agccgctttt tctgcacaca cacgcccagt tcaaacggcg ttttctcatc
     2701 actgcgctct aaaatcatca cgcggttacg caccggttcc gggctgtttt ccagcaccac
     2761 ttccggcaac aaggctacgc cacagccaag cgccaccatc gacaccatcg cttcatgccc
     2821 gccgaccgtg gcgtaaatca tcgggttgct gattttgttg cgtcgaaacc acagttcaat
     2881 gcgccggcgc accggtccct gatcggccat gataaacggc actgtagacc agtccggctt
     2941 ctctgccgaa acttggttac gcaccgggca gggtagcgcg ggggcaatca gcactactgc
     3001 cagattctcc agcatcgaaa acgccactgc gccgggcaga gtctccggtt tcccggcaat
     3061 cgccagatcc gcttcgccag tgactacctt ttccatcgca tctgccgcat caccagtcgt
     3121 gagtttaatc tccaccgacg ggtgttccgc gcggaagcga tcgagaatcg gcggcagatg
     3181 gctgtaggca gcggtcaccg agcagaagat atgtaattcg ccagagagcg acggtccttg
     3241 ctgatcgatg gtgtggcgca gctgttgata ctgcaacagc gtttgctggg cgaaaacgcg
     3301 cagttcttcg cctgcttcgg ttagcgttac cgtgcggtta tcgcgcacaa acagcggctg
     3361 accgagatcg tcttccaggc gctgaatctg ccgagagggc gnnnnnnnnn ncgtggacgg
     3421 gctaacgtgc attgcccgcg cgctgcggcc aaaatggcgg ctttccgcca gatgcaggaa
     3481 ggttttcaga tcgcgtaaat ccaatggcgc agcccttaat gttgacgttg cagaaattgc
     3541 aatgtgacgt tgtgaatata tcaatttccg caataaattt cctgtcatat agtgaattca
     3601 atctcgcaaa cgcgaaccga acaataagaa gcacaacatc acgaggaatc accatggcta
     3661 actacttcaa tacactgaat ctgcgccagc agttggcaca gctgggcaaa tgtcgcttta
     3721 tggggcgcga tgaattcgcc gatggcgcga gctaccttca gggtaaaaaa gtagtcatcg
     3781 tcggctgtgg cgcacagggt ctgaaccagg gcctgaacat gcgtgattct ggtctcgata
     3841 tctcctacgc tctgcgtaaa gaagcgattg ctgagaagcg cgcatcctgg cgtaaagcaa
     3901 ccgaaaatgg ttttaaagtg ggtacttacg aagaactgat cccgcaggcg gatctggtgg
     3961 ttaacctgac gccggacaag cagcactctg atgtagtgcg caccgtacag ccactgatga
     4021 aagacggcgc ggcgctgggt tactcacacg gtttcaacat cgtcgaagtg ggcgagcaga
     4081 tccgtaaaga catcaccgtc gtaatggttg cgccgaaatg ccctggcacc gaagtacgtg
     4141 aagagtacaa acgtggattc ggcgtaccga cgctgattgc cgttcacccg gaaaacgatc
     4201 cgaaaggcga aggcatggcg atcgctaaag catgggcggc tgcaaccggc ggtcaccgtg
     4261 cgggcgtgct ggaatcgtcc ttcgttgcgg aagtgaaatc tgacctgatg ggtgagcaaa
     4321 ccattctgtg cggtatgttg caggctggtt ctctgctgtg cttcgacaag ctggtggaag
     4381 aaggcaccga tccggcatac gcagaaaaac tgattcagtt cggctgggaa accatcaccg
     4441 aagcactgaa acagggcggc atcacgctga tgatggaccg tctctctaac ccggcgaaac
     4501 tgcgtgctta cgcgctttct gaacagctga aagagatcat ggcaccgttg ttccagaaac
     4561 atatggacga tatcatctcc ggcgaattct cctccggcat gatggcagac tgggccaacg
     4621 acgataagaa actgctgacc tggcgtgaag agaccggcaa aaccgcgttc gaaaccgcgc
     4681 cgcagtatga aggcaaaatc ggcgaacagg agtacttcga taaaggcgta ctgatgatcg
     4741 cgatggtaaa agcgggcgtt gagctggcgt ttgaaaccat ggttgattcc ggcgtcatcg
     4801 aagagtctgc ttactatgaa tcactgcacg aactgccgct gattgccaac actatcgctc
     4861 gtaagcgtct gtacgaaatg aacgtggtta tctctgatac cgccgagtac ggtaactatc
     4921 tgttctctta cgcttgtgtg ccgctgctga aaccgtttat ggcagagctg caaccgggcg
     4981 acttgggtaa ggctattcct gaaggtgcgg tagataacgc gcagttgcgt gatgtgaacg
     5041 aagcgattcg cagccatgcg attgagcagg taggtaagaa actgcgcggc tatatgacgg
     5101 atatgaaacg tattgctgtt gcaggttaag tgcgattcag agttcaaggc cttctcccgg
     5161 agaaggcctt gctgttagtt acggtaaagc accttaatga tgtggtaacc gaactgggta
     5221 tgcagcgggc cggttggctc cagtaccgga caagagaaaa ccactttatc gaacgccgga
     5281 accatttgac cctggcggaa ttcacccaaa tcaccgccgc gtttgcctga cgggcaaatg
     5341 gagtgtttct tcgccagctt gccgaaatcg gcaccattct tgatctgctc gagaagatcc
     5401 agagccagtt tctcttcttt tacaaggata tgcagtgctg ctgctgtttt tgccatgatc
     5461 gtgccttgag tggtaaggat gaggctggct atactaccac gctgttattt tcccctcccg
     5521 actttcattg agcgattttc ctatgcgtct aaaccccggc caacaacaag ctgtcgaatt
     5581 cgttaccggc ccctgcctgg tgctggcggg cgcgggttcc ggtaaaactc gtgttatcac
     5641 caataaaatc gcccacctga tccgcggttg cggttatcag gcgcgacata tcgcggcggt
     5701 gacctttacc aataaggcgg cgcgcgagat gaaagagcgt gtcgggcaga cgctggggcg
     5761 caaagaggcg cgtgggctga tgatctccac cttccacacg ctggggctgg atatcatcaa
     5821 acgcgagtat gcggcgctgg ggatgaaggc taacttttcg ctgtttgacg ataccgatca
     5881 gcttgctttg cttaaagagt tgaccgaggg g
//
LOCUS       DAWDRC010000201         5911 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6139_length_5911_cov_2.071892, whole genome shotgun sequence.
ACCESSION   DAWDRC010000201 DAWDRC010000000
VERSION     DAWDRC010000201.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6139_length_5911_cov_2.071892"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4879..4888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1276 a         1633 c         1476 g         1516 t
ORIGIN      
        1 accacgtgaa tctgtgatcc cagcgcgtgc tatacggtgc ccatttccag accgatgata
       61 ccgccaccca ttaccagcag gcgttctggt acttctttca gttccagcgc gtcagtggag
      121 tcccagatac gcggatcttc atgcggaata aacggcagtt gaatcgggcg agaacccgct
      181 gcaatgatcg cgttgtcgaa gttgatcacg gttttaccgt tctcaccttc aacttccagg
      241 gtgttagccc cggtaaattt acccagaccg ttgaccactt tgactttgcg gcctttcgcc
      301 ataccagcca gaccaccggt cagctgattg atcactttct ctttccaggt acgaatcttg
      361 tcgatatcgg ttttcggttc gccaaagacg ataccgtgtt cagccagcgc tttggcttct
      421 tcgataactt ttgctacgtg cagcagtgct ttagaaggga tacagccgac gttcaggcaa
      481 acaccgccga gggtgttgta gcgttctacg attacggttt ccagacctaa atcagcgcaa
      541 cggaaggcag cggagtaacc tgcggggcct gccccaagta ccacgacctg agttttgatt
      601 tcagtactca tcatgacctc tatatattta tctccggcgg gtctgacgtt ttttttatga
      661 cgcccattat ccaccgggtc gttctatccg tcggtatttt acaaaattgt taataatttt
      721 taaacaacaa acggcaaccg atttgtctat tcgctaataa tctcaataca ttcatgagat
      781 taccagaaaa gagccggccg ttgggccggc tcttttattt acatcaccag acggcgaatg
      841 tcagacagcg tgttgttaat gatggtaatg aaacgggcac catcagcacc gtcgatcacg
      901 cggtggtcga aggagagaga aatcggcagc atcagacgcg gcacgaactc tttaccattc
      961 cacaccggct ccatcgcgga cttggaaacg ccgaggatag ccacttccgg cgcgttcaca
     1021 atcggcgcga agtgggtagt acccaggccg ccgatgctgg agatggtgaa gcaaccgccc
     1081 tgcatttcgc ccgcagtcag cttaccgtca cgcgctttct tagaaatagt catcagctcg
     1141 cgagacagct cgatgatgcc tttcttgttg acgtctttga ataccgggac aaccagaccg
     1201 ttcggggtat ccaccgccac accgatgttg atgtatttct tcagggtcag acgctgaccg
     1261 tcttccgaca gcgaactgtt gaagcgaggc atctgctcaa gagctgcagc aacggctttc
     1321 atgatgaaga caaccggggt gatcttcaca tccagcttac gtttcgccgc ttcttcgttc
     1381 tgctgtttac ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg agtaacatgc
     1441 gggatcatca cccagttacg gctcaggttc gcaccagaga ttttctggat gcggcccagt
     1501 tccacttctt cgatttcacc aaacttgctg aagtccacct tcggccacgg cagcatgcct
     1561 gggataccgc cgccagttgc cgccggagct gcttctgcac gtttgatagc ttctttcacg
     1621 taagcctgaa cgtcttcgcg caggatacga cctttacggc cggtgccctt cactttcgcc
     1681 aggttaacgc caaactcgcg tgccagacgg cggatcagcg gagtcgcgtg aacgtaagcg
     1741 tcattttcag caaattcaga tttgccttcg gcttttgcag ccggagcttc ctgtttcgca
     1801 ggagctgccg caggcgctgc gccttcaact tcgaacatca taatcagcga accagttttc
     1861 actttatcgc caacgttgac tttcagttcc ttcacgacgc ctgcaaacgg agccggaact
     1921 tccatagaag ctttatcgcc ttctacggtg atcagtgact gttcagcggc aactttgtcg
     1981 cccactttca ccatcacttc ggtcacttca acttcgtcac cgccgatatc cggaacgtta
     2041 acttctttca cgccagcggc tggtgcaggg gccgctgccg gggccgcttc ctgtttagcc
     2101 gccggagctg ccgcgcctgc ttcacccgcg acttcgaaga tcataatcag cgagccggta
     2161 gacactttgt cacccacgtt cactttgatc tctttcacgg tgcctgcaaa cggagcagga
     2221 acttccatag aagccttgtc gccttctacg gtgatcagcg actgttcagc ttcaacttta
     2281 tcgccaactt tcaccaggat ttcggtcact tcaacttcgt cgctgccaat atccggaacg
     2341 ttaacgtctt ttgctgccgc agccgctggt gctgctgccg gagctgcttc tttcttctct
     2401 tctgcctgag caggtgcagc gtctgctgca ccgtcggcgg aatcgaaaat cataatcagt
     2461 gcgccggtct gggttttatc gccaacagag actttgatct ctttaacgat acccgcctgc
     2521 ggagatggta cttccataga ggctttgtcg ccttctacgg tgatcagcga ctgttcggct
     2581 tcaactttgt cgcccacttt gaccaggatc tcggtgattt caacttcatc agccccgatg
     2641 tccggtactt tgatttcgat agccattatt cttttacctc ttacgccaga cgcgggttaa
     2701 ctttatctgc atcgatgttg aatttggcga ttgcgtcagc aaccactttc ttatcgattt
     2761 cgccacgttt agccagttcg cccagcgccg caaccacgac ataagaagca tcaacttcaa
     2821 agtggtgacg caggttctca cggctgtcgg aacgaccgaa gccatcagta cccagtacgc
     2881 ggtagtcgtc agccggtacg taagtacgga cctgctcagc gaacagtttc atatagtcgg
     2941 tagatgccac tgccggagcg tcgttcatca cctgagcgat atacggaacg cgcggagttt
     3001 ccagcgggtg cagcatgttc cagcgttcac aatcctgacc atcacgcgcc agttcggtga
     3061 aggaagtcac gctataaacg tcagaaccta cgccgtaatc tttcgccagg atctctgccg
     3121 cttcacggac gtgacgcagg atagaaccgg agcccagcag ctgaacttta cctttgctac
     3181 cttcaatggt ttcgagtttg tagatacctt tacggatacc ttcctcagca ccttccggca
     3241 ttgccggcat gtggtagttt tcgttcagcg tagtgatgta gtagtaaacg ttctcttgtt
     3301 tttcaccata catacgctcc agaccgtcat gcatgatgac agcaacttcg taagcgtaag
     3361 ccgggtcgta agagatacag ttcgggatag tcagcgactg aatatggctg tgaccatctt
     3421 cgtgctgcag accttcgccg ttcagggtgg tacgaccgga agtaccgccg atcaggaagc
     3481 cacgcgcttg ctggtcgcca gccgcccagc acagatcgcc gatacgctgg aaaccgaaca
     3541 tcgagtaata gatgtagaac gggatcatcg gcagattgtt ggtgctgtaa gaggtcgccg
     3601 ctgccagcca ggaacaacct gcgcccagct cgttgatccc ttcctgcaga atctgacctt
     3661 tctcgtcttc tttatagtaa gcaacctgct cgcggtcctg cggggtgtac tgctgaccgt
     3721 tcgggctgta aataccaatc tgacggaaca gaccttccat accgaaagta cgcgcttcgt
     3781 cggcgatgat cggtaccagc ctgtctttga tcgacttgtt cttcagcatt acgttcaggg
     3841 cacgaacgaa agcgatagtg gtagagatct ctttgctctg ctcttccagc agcgcgccga
     3901 agtcttgcag gctcggcagc tcaagcttct cggtgaagtt cggctgacgg cttggcagat
     3961 aaccgtgcag tttctgacgc tgagcgtgca gataggtatg ctcttcagaa ccttccggga
     4021 aggtgatgta cggcagtttt tcgatatctg catcagacac cggcacattg aaacggtcgc
     4081 ggatatgacg cacgccgtcc atgttcattt tcttaacctg gtgcgcgatg tttttacctt
     4141 cagccgcgtc gcccatgcca taacctttaa tggtatgagc aaggattacc gtcgctttgc
     4201 ctttggtttc ctgcgctttc ttgaatgcag cgtagatttt cttcggatcg tgaccaccac
     4261 ggttcagtgc ccagatctgc tcgtcagtcc agtctgcaac cagtgctgcg gtttcaggat
     4321 atttaccgaa gaagtgttcg cgaacatacg caccatcttt cgatttgaag gtctggtaat
     4381 cgccgtcaac ggtttcgttc atcagctgga tcagtttacc gctggtatct ttacgcagca
     4441 gttcgtccca acggctaccc cacatcactt tgatcacgtt ccagccagca ccttcgaaga
     4501 tgccttccag ttcgttgatg atcttgccgt taccggtgac cgggccgtca agacgctgca
     4561 ggttacagtt gataacgaag accagattat ccagtttttc acgggtagcg atggtgatcg
     4621 cacctttgga ttccggttcg tccatttcac cgtcgccgag gaacgcgtaa acggtttgtt
     4681 tagaggtatc tttcaggcca cggtgttcca gatatttcag gaatttagcc tggtaaatag
     4741 caccaatcgg acccagaccc atagatacgg tcgggaactg ccagaattcc ggcatcagtt
     4801 tcgggtgcgg ataggaagag aggccattgc cgtgaacttc ctgacggaag ttatccagct
     4861 gctcctgagt cagacgacnn nnnnnnnnag acgaccttcc aggaaagcac gagcgtacac
     4921 gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct cgttgcgtgc
     4981 acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga aggacgccat
     5041 gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga tggcgttcca
     5101 gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact ccggttgttc
     5161 ttcaacgggg atggtgttga tgtagttgct gataccggtg cctgcagcta cgttgactcc
     5221 gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct caacaccttc
     5281 ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg gatccacgtc
     5341 atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt gagttttctg
     5401 gaacctgtcc cattgaactc tcgccggaaa gctcaataag acaggttcta cgtttagttg
     5461 ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac cagaaaaatc
     5521 actaattctt tcgttgctcc agacgacgta gagaacgctc acggcggctc tcttcacggc
     5581 ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct tcgcgcgctt
     5641 cttccggctt accggccata atcgcttcaa atatgcgggt gcggtgacta ctcaccagcg
     5701 gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc tgggccaaca
     5761 tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct tcggtgacgg
     5821 caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac tgctgcgcca
     5881 gctctatggc atggtggagt tcacggatgc g
//
LOCUS       DAWDRC010000202         5911 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6137_length_5911_cov_2.581284, whole genome shotgun sequence.
ACCESSION   DAWDRC010000202 DAWDRC010000000
VERSION     DAWDRC010000202.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6137_length_5911_cov_2.581284"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1421 a         1574 c         1427 g         1489 t
ORIGIN      
        1 gtacatcgtc ctgagaacgg cgatcgtcgt cttcgctgat ctctttatct ttcaacagtg
       61 ctttcacttt gtcgttcgcg tcacgacgca cattacgtac tgcaacacgc gcttgttctg
      121 cttcaccacg aacgattttg gtcagatctt tacgacgttc ttccgtcagc ggcggcagcg
      181 gaacacggat gtcgctaccc gcagagttcg ggttcaggcc aagatcggac gccataatcg
      241 ctttttcaac ggccggagac attgaacgat caaacacgtt gattttcaga gtacgggaat
      301 cttctaccgt tacgcttgcc agctgacgca gcggcgtcgg cgtgccgtaa tattccacga
      361 caatgccatc cagcaggctg ggagaagcac gacccgtgcg tattttgctg atttgggttt
      421 tgaacgcttc tacgcattta tccatgcgta cttcagcatc ttttctgata tcgctaatca
      481 cgttacgaat ccttgaaaac ttgtctcagt cagaccagac agtcacacac agtggaagtg
      541 tgctaagtat agccctgatt aaacatatta cgggtaagat cccccgcgat acttacgcgg
      601 aatcttaccc ttatttatcc atcacgggaa ttattccgtg attaaagtcc cttctttttc
      661 acccattacc acacggcgca gcgcacccgg tttcttcatg ttgaaaacac gaatcggtaa
      721 tttatggtca cgagcaagcg tgaaggccgc caggtccatg actttcagct ctttttccag
      781 cacttcgctg taagtcagtt gctcgtacat ggttgcggtt ggatctttcg ccggatcagc
      841 agtaaacacg ccgtcaactt tggttgcttt cagcaccaca tcggcttcaa tttcgatacc
      901 acgcaggcaa gctgctgagt cggtggtaaa gaacgggtta cctgtaccgg cggagaggat
      961 caccacacgg ttgttgcgca acaggctgat agcttctgcc cagctgtagc tgtcgcacac
     1021 gccattcaat ggaatagcgg acatcagacg agcgttcaca taggcgcggt gcagtgcatc
     1081 acgcattgcc aggccgttca ttacggtcgc cagcatcccc atgtggtcgc ccacaacgcg
     1141 gttcataccc gctttcgcca gaccagcgcc acggaacagg ttacccccac caatcaccac
     1201 accaacctga atacccagtt caaccagttc tttgatttcc tgagccatgc gatccagtat
     1261 gcttgcatca ataccgaagc cttcagtgcc ctgcagagct tcgccactca acttaagcag
     1321 aatgcgttta tagacgggtt ttgcattggt agccatgttt ctttcctgag actgtcaacg
     1381 attgaaatga attaatacta gcgacattgt acgtcgcttt tcagcgcagc cccacaaggg
     1441 ttagctgaat ttacaagatg ggcacaaaaa gaagccgccc tcaggcggct ccttttcgat
     1501 aattaagact gcttggacat cgcagcaact tctgctgcaa agtcagtctc aactttctcg
     1561 atgccttcac ccacttcgaa gcggatgaag ccagtcactt cagcgttatg ctctttcagc
     1621 agctgaccaa cagttttgct tggttccata acgaacggct gaccggtcag agaaacttcg
     1681 ccggtgaatt tcttcatgcg gccttcaacc attttctctg cgatttcttt cggcttacca
     1741 gactgcatcg cgatatccag ctgtacctgg tattcttttt ctaccacttc agcggatacg
     1801 tcttctggtt tgatgaattc tggcttgctt gcagcaacgt gcatagcgat gtgtttaacc
     1861 agctcttcgt cagcgccttt agcagcaacc agaacaccga tacgcgcacc gtgctgataa
     1921 gaacccagaa cgtcgccttc cagcgcagca acgcggcgaa tgttgatgtt ttcaccaatt
     1981 ttcgctacca gcgcaacacg ttcttcttcg aactgtgctt tcagtacttc aacgtcagtg
     2041 attttgccag caacagctgc gtccagaact ttgtctgcga acgcctggaa accagcgtct
     2101 tttgcaacga agtcagtctg gcagttaact tccagaatga tgccgtagtt gccgtcgatt
     2161 ttggttttga tcacgccatc agcagcaacg ttgcctgctt ttttcgctgc tttaatagca
     2221 ccggacttac gcatgttttc gattgccagc tcgatgtcgc cgttagcttc agtcagtgct
     2281 tttttgcaat ccatcatgcc tgcgccagta cgctcacgca gctcttttac cagggatgcg
     2341 gtaatttcag ccattctaaa atcctcggag atgtgatctg cccggcatta aaccgcacag
     2401 atataaaagt gaaaaagggg gccatatgca ggccccctaa ccaaacgtgt actacctggt
     2461 ctataagggc tcgtacgagc gtgccttatt actcagcttc tacgaagctt tcttccgcct
     2521 gggaagccag atcctgagaa cggccttcac gtacggttgc agcaacagcg cccaggtaca
     2581 gggtcacagc acggattgcg tcgtcgttac ccgggataac gaagtcaaca ccgtccggat
     2641 cagagttggt atcaacgata gcaaacactg gaatacccag gttgtttgct tctttgatag
     2701 caatgtgttc gtggtcagca tcgattacaa acagagcgtc cggcagaccg cccatgtctt
     2761 tgataccgcc caggctgttt tccagtttct ccagctcacg agtgcgcatc agcgcttctt
     2821 tcttggtcag cttgtcgaaa gtaccgtcct gagactgagt ttccaggtct ttcagacgtt
     2881 tgatggactg acgaacggtt ttccagttag tcagcatacc gcccagccag cgatggttca
     2941 cgaagaactg gtcgcagctc agagcagcgt ctttcaccgc ttcgcttgca gcgcgtttag
     3001 taccaacgaa aaggatttta cctttgcgag aagcaatctt gttcagttca gccagagctt
     3061 cgttgaacat cggtacagtt ttctcaaggt tgatgatgtg aactttgtta cgcgcaccga
     3121 agatgaacgg cttcattttc gggttccagt aacgggtctg gtgaccgaag tgaacaccag
     3181 ccttgagcat gtcgcgcatg gaaacagttg ccatgattaa aacctctata taaaagttgg
     3241 ggttatgcct ccacgtatcc catattaccg accccaaagg gcaccccgga atatgtgccg
     3301 atacgtgtgt gttgttacac aaagtgagat tgtcgcttcc gtccagtctg tgtatacgaa
     3361 atggatcgga agtccggcgc gctttatacc acaaatacgt cgtggacacc aataattgtt
     3421 ggcgctgtgt acagcatcag acgttgaatt ttctattata gaaaaccttc agtggcacgt
     3481 ttggcgaaat tcagaatgat tctcaatttg cccgggtgtg ataccattga cggcacttac
     3541 atatatattg tcggtatcac cgacgctgat ggacagaatt aatggctatc tcaatcaaga
     3601 ccccagaaga tatcgaaaaa atgcgcgtcg ctggccgact ggctgccgaa gtgctggaga
     3661 tgatcgaacc gtatgttaaa ccgggcgtca gcaccggcga gctggatcgc atctgtaatg
     3721 attacattgt taatgaacaa cacgcggttt ctgcctgcct cggctatcac ggctatccga
     3781 aatccgtttg catctctatt aatgaagtgg tgtgccacgg tatcccggac gacgccaagc
     3841 tgctgaaaga tggcgatatc gttaacattg atgtcaccgt aatcaaagat ggtttccacg
     3901 gcgatacctc gaaaatgttt atcgtcggta agccgaccat catgggcgaa cgtctgtgcc
     3961 gcatcacgca agaaagcctg tacctggcgc tacgcatggt aaaacctggc attaatctgc
     4021 gcgaaatcgg tgcggcaatt cagaaatttg tcgaagcaga aggcttctcc gtcgttcgtg
     4081 aatattgtgg acacggtatt ggtcgcggct tccacgaaga accgcaggtg ctgcactatg
     4141 actcccgtga aaccaacgtc gtgctgaaac cggggatgac gttcaccatc gagccaatgg
     4201 tcaacgcggg taaaaaagag atccgcacca tgaaagatgg ctggacggta aaaaccaaag
     4261 atcgcagctt gtctgcacaa tatgagcata ctattgtggt gactgataac ggctgcgaaa
     4321 ttctgacgct acgcaaggat gacaccatcc cggcgataat ctcgcacgac gaataaaatg
     4381 aagccggcga atgccggctt ttttaatgca ataatttaat cttatgggtg gcgcacaatg
     4441 aatacccttc cagaacagta cgcaaacacc gctctcccca ccctgcccgg tcaaccgcaa
     4501 aatccatgcg cctggccccg tgatgaatta accgtcggtg gaataaaagc ccatatcgat
     4561 actttccagc gttggctggg tgatgccttt gacaatggta tctccgcaga acagttgatt
     4621 gaggcgcgta ccgagtttat cgaccagctc cttcaacgat tatggattga agcgggattc
     4681 agccagattg ccgacctggc attggtcgcc gtcggtggct acggtcgtgg cgagctgcat
     4741 ccactttctg acatcgattt actgatttta agccgtaaaa agctcccgga cgatcaggcg
     4801 caaaaagtgg gcgagctgtt aacgctgctc tgggatgtaa agctggaagt gggtcatagc
     4861 gtgcgcacgc ttgaagagtg catgctggaa gggttatcgg atttaaccgt cgccaccaat
     4921 ttaatcgaat cccgcttatt aattggcgat gttgcgctgt tcctcgaact gcaaaaacat
     4981 atttttagcg aaggattctg gccttccgac aagttctacg cggcgaaagt tgaagaacag
     5041 aaccagcgcc atcagcgtta ccacggcacc agctacaacc ttgaaccaga catcaaaagt
     5101 agccccggcg gcttgcgcga tatacacact ctgcagtggg tggcccgccg tcattttggc
     5161 gcaacatcgc tggatgaaat ggtagggttt ggcttcttaa cctctgcgga gcgagcggaa
     5221 ttaaacgaat gcctgcatat attgtggcgc attcgctttg ccctgcatct ggtcgttagt
     5281 cgttacgata atcgcctgtt attcgatcgc cagcttagcg tcgcccagcg tttgaattac
     5341 agtggtgaag gtaacgaacc ggtcgagcgg atgatgaagg attacttccg cgttacacgc
     5401 cgcgtcagtg aactcaacca gatgctgctg caactgttcg atgaagccat cctcgccctt
     5461 cccgccgacg aaaaaccacg tccaatcgac gatgagtttc agctacgcgg aacgctgatc
     5521 gacctgcgtg atgaaacact atttatgcgc cagccggaag ccatcttgcg tatgttctac
     5581 accatggtgc gcaacagtgc gatcaccggc atttactcca ccacgctacg ccagttacgc
     5641 cacgcccgtc gccatctgca acaacctctg tgtaatattc cggaagcgcg aaaactgttt
     5701 ttgagcattc ttcgtcaccc cggagcggtg cggcgcgggc tattgccaat gcatcgccat
     5761 agcgtactcg gcgcgtatat gccgcaatgg tcgcatatcg tcgggcagat gcagtttgat
     5821 ctgttccacg cctacacggt ggatgaacat actatccgcg tgatgctgaa actggagagt
     5881 tttgccagtg aagaaacgcg ccagcgccat c
//
LOCUS       DAWDRC010000203         5907 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6143_length_5907_cov_3.123889, whole genome shotgun sequence.
ACCESSION   DAWDRC010000203 DAWDRC010000000
VERSION     DAWDRC010000203.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6143_length_5907_cov_3.123889"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1576 a         1346 c         1304 g         1681 t
ORIGIN      
        1 catatcaacg gacctccacg gaggtccgtt tttcgtttca ggacgccacg atttaagcgt
       61 cctgccgcca aatcaattct accgaactca accagattct ccccacatca ccagcaattt
      121 gcgggcatat cccaattcgg gaaaatttgt ttctgagcta tagcgctgac tgacgtgaaa
      181 tgtcgtgcgg ccccgtgatg ctgttgaacg tcaaatgacg tcatcaggag cgtaacgcac
      241 ccataaagca caacatcggg cagaacgcca actgatgaga ttttctgaat gagaacaaag
      301 agaaatgtat cagtccgttt gctcatgcaa agactaacaa tccattaaaa tagtaagcgc
      361 tccggacaat ttttcatgga ttattttctg aacatttttc tttggcaaag atgatgaatt
      421 ttgatggtaa ggaaaattac ttctggttct cagtaaaatc ctttcgtaat actatgtaat
      481 caagaagttt atggcgggta aaaataacgt cctacattca ccaataatat gtaaataaac
      541 ccatctatag atggaaaaaa taggttatgg caattatcat tgcatcattc ccttttcgaa
      601 tgagtttcta ttatgcaaca acctgtagtt cgcgttggcg aatggcttgt tactccgtcc
      661 ataaaccaaa ttagccgcaa tgggcgtcaa cttacccttg agccgagatt aatcgatctt
      721 ctggttttct ttgctcaaca cagtggcgaa gtacttagca gggatgaact tatcgataat
      781 gtctggaaga gaagtattgt caccaatcac gttgtgacgc agagtatctc agaactacgt
      841 aagtcattaa aagataatga tgaagatagt cctgtctata tcgctactgt accaaagcgc
      901 ggctataaat taatggtgcc ggttatctgg tacagcgaag aagagggaga ggaaataatg
      961 ctatcttcgc ctccccctat accagaggcg gttcctgcca cagattctcc ctcccacagt
     1021 cttaacattc aaaacaccac aacgccacct gaacaatccc cagttaaaag caaacgattc
     1081 actacctttt gggtatggtt ttttttcctg tagtcgttag gtatctgtgt cacactggta
     1141 gcgttttcaa gtcttgaaac acgtcttcct atgagtaaat cgcgcatttt gctcaatcca
     1201 cgcgatattg acattaatat ggttaataag agttgtaaca gctggagttc tccgtatcag
     1261 ctctcttacg cgataggcgt gggtgatttg gtggcgacat cacttaacac cttctccacc
     1321 tttatggtgc atgacaaaat caactacaac attgatgaac cgagcagttc cggtaaaaca
     1381 ttatctattg cgtttgttaa tcagcgccaa taccgtgctc aacaatgctt tatgtcggta
     1441 aaattggtag acaatgcaga tggttcaacc atgctggata aacgttatgt catcactaac
     1501 ggtaatcagc tggcgattca aaatgatttg ctccagagtt tatcaaaagc gttaaaccaa
     1561 ccgtggccac aacgaatgca ggagatgctc cagcaaattt tgccgcatcg tggtgcgtta
     1621 ttaactaatt tttatcaggc acatgattat ttactgcatg gtgatgataa atcattggat
     1681 cgtgccagtg aattattagg tgagattgtt caatcatccc cagaatttac ctacgcgaga
     1741 gcagaaaaag cattagttga tatcgtgcgc cattctcaac atcctttaga cgaaaaacaa
     1801 ttagcagcac tgaacacaga aatagataac attgttacac tgccggaatt gaacaacctg
     1861 tccattatat atcaaataaa agcggtcagt gccctggtaa aaggtaaaac agatgagtct
     1921 tatcaggcga taaataccgg cattgatctt gaaatgtcct ggctaaatta tgtgttgctt
     1981 ggcaaggttt atgaaatgaa ggggatgaac cgggaagcag ctgatgcata tctcaccgcc
     2041 tttaatttac gcccaggggc aaacaccctt tactggattg aaaatggtat attccagact
     2101 tctgttcctt atgttgtacc ttatctcgac aaatttctcg cttcagaata agtaactccc
     2161 gggttgattc atgctcggga atatttgttg ttgagttttt gtatgttccc gttggtataa
     2221 tatgttgcgg caatttattt gccgcataat ttttattaca taaatttaac cagaaaatgt
     2281 cacataacat attgtaaaca ttgaatgttt atcttttcat gatatcaact tgcgatcctg
     2341 atgtgttaat aaaaaacctc aagttctcac ttacagaaac ttttgtgtta tttcacctaa
     2401 tctttaggat taatcctttt ttcgtgagta atcttagcgc cagtttggtc tggtcaggaa
     2461 atagttatac atcatgaccc ggactccaaa ttcaaaaatg aaattaggag aagagcatga
     2521 gttctgccaa gaagatcggg ctatttgcct gtaccggtgt tgttgccggt aatatgatgg
     2581 ggagcggtat tgcattatta cctgcgaacc tagcaagtat cggtggtatt gctatctggg
     2641 gttggattat ctctattatt ggtgcaatgt cgctggcata tgtatatgcc cgactggcaa
     2701 caaaaaaccc gcaacaaggt ggcccaattg cgtatgccgg agaaatttct cctgcatttg
     2761 gttttcagac aggtgttctt tattaccatg ctaactggat tggtaacctg gcaattggta
     2821 ttaccgctgt atcttatctt tccaccttct tcccagtatt aaatgatcct gttccggcgg
     2881 gtatcgcctg tattgctatc gtctgggtat ttacctttgt aaatatgctc ggcggtacct
     2941 gggtaagccg tttaaccact attggtctgg tgctggttct tattcctgtg gtgatgactg
     3001 ctattgttgg ctggcattgg tttgatgcag caacttatgc agctaactgg aatactgcgg
     3061 ataccactga tggtcatgcg atcattaaaa gtattctgct ctgcctgtgg gccttcgtgg
     3121 gtgttgaatc cgcagcagta agtactggta tggttaaaaa cccgaaacgt accgttccgc
     3181 tggcaaccat gctgggtact ggtttagcag gtattgttta catcgctgcg actcaggtgc
     3241 tttccggtat gtatccgtct tctgtaatgg cggcttccgg tgctccgttt gcaatcagtg
     3301 cttcaactat cctcggtaac tgggctgcgc cactggtttc tgcattcacc gcctttgcgt
     3361 gtctgacttc tctgggctcc tggatgatgt tggtaggcca ggcaggtgta cgtgccgcta
     3421 acgacggtaa cttcccgaaa gtttatggtg aagtcgacag caacggtatt ccgaaaaaag
     3481 gtctgctgct ggctgcagtg aaaatgactg ccctgatgat cctcatcact ctgatgaact
     3541 ctgccggtgg taaagcatct gacctgttcg gtgaactgac cggtatcgca gtactgctga
     3601 ctatgctgcc gtacttctac tcttgcgttg acctgattcg ttttgaaggc gttaacatcc
     3661 gcaactttgt cagcctgatc tgctctgtac tgggttgcgt gttctgcttc atcgcgctga
     3721 tgggcgcaag ctccttcgag ctggcaggta ccttcatcgt cagcctgatt atcctgatgt
     3781 tctacgctcg caaaatgcac gagcgccaga gccactcaat ggataaccac acagcgtcta
     3841 acgcacatta attaaaagta ttttccgagg ctcctccttt cattttgtcc catgtgttgg
     3901 gaggggcctt ttttacctgg agatatgact atgaacgtta ttgcaatatt gaatcacatg
     3961 ggggtttatt ttaaagaaga acccatccgt gaacttcatc gcgcgcttga acgtctgaac
     4021 ttccagattg tttacccgaa cgaccgtgac gacttattaa aactgatcga aaacaatgcg
     4081 cgtctgtgcg gcgttatttt tgactgggat aaatataatc tcgagctgtg cgaagaaatt
     4141 agcaaaatga acgagaacct gccgttgtac gcgttcgcta atacgtattc cactctcgat
     4201 gtaagcctga atgacctgcg tttacagatt agcttctttg aatatgcgct gggtgctgct
     4261 gatgatattg ctaacaagat caagcagacc actgacgaat atatcaacac tattctgcct
     4321 ccgctgacca aagcactgtt taaatatgtt cgtgaaggta aatatacttt ctgtactcct
     4381 ggtcacatgg gtggtactgc attccagaaa agcccggtag gtagcctgtt ctatgatttc
     4441 tttggtccga acaccatgaa atctgatatt tccatttcag tatctgaatt gggttctctg
     4501 ctggatcaca gtggtccaca caaagaagca gaacagtata tcgctcgcgt ctttaacgca
     4561 gaccgcagct acatggtgac caacggtact tccactgcga acaaaattgt tggtatgtac
     4621 tctgctccgg caggcagcac cattctgatt gaccgtaact gccacaaatc gctgacccac
     4681 ctgatgatga tgagcgatgt tacgccaatc tatttccgcc cgacccgtaa cgcttacggt
     4741 attcttggtg gtatcccaca gagtgaattc cagcacgcta ccattgctaa gcgcgtgaaa
     4801 gaaacaccaa acgcaacctg gccggtacat gctgtaatta ccaactctac ctatgatggt
     4861 ctgctgtaca acaccgactt catcaagaaa acactggatg tgaaatccat ccactttgac
     4921 tccgcgtggg tgccttacac caacttctca ccgatttacg aaggtaaatg cggtatgagc
     4981 ggtggccgtg tagaagggaa agtgatttac gaaacccagt ctactcacaa actgctggcg
     5041 gcgttctctc aggcttccat gatccacgtt aaaggtgacg taaacgaaga aacctttaac
     5101 gaagcctaca tgatgcacac caccacctct ccgcactacg gtatcgtggc gtccactgaa
     5161 accgctgcgg cgatgatgaa aggcaatgca ggtaaacgtc tgatcaatgg ttccattgaa
     5221 cgtgcgatca aattccgtaa agagatcaaa cgtctgagaa cggaatctga tggctggttc
     5281 tttgatgtat ggcagccgga tcatatcgat acgactgaat gctggccgct gcgttctgac
     5341 agcacatggc acggcttcaa aaacatcgat aacgagcaca tgtatcttga cccgatcaaa
     5401 gtcaccctgc tgactccggg gatggaaaaa gacggcacca tgagcgactt tggtattccg
     5461 gccagcatcg tggcgaaata cctcgacgaa catggcatcg ttgttgagaa aaccggtccg
     5521 tataacctgc tgttcctgtt cagcatcggt atcgataaga ccaaagcact gagcctgctg
     5581 cgtgctctga ctgacttcaa acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg
     5641 ccgtctctgt atcgtgaaga tcctgaattc tatgaaaaca tgcgtattca ggaactggct
     5701 cagaatatcc acaaactgat tgttcaccac aatctgccgg atctgatgta tcgcgcattt
     5761 gaagttctgc cgactatggt aatgactccg tatgctgcgt tccagaaaga gctgcacggt
     5821 atgaccgaag aagtttacct cgacgaaatg gtcggtcgta ttaacgccaa tatgatcctt
     5881 ccgtatccgc cgggagttcc tctggta
//
LOCUS       DAWDRC010000204         5903 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6147_length_5903_cov_2.485978, whole genome shotgun sequence.
ACCESSION   DAWDRC010000204 DAWDRC010000000
VERSION     DAWDRC010000204.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_5903_cov_2.485978"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5568..5577
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1366 a         1413 c         1558 g         1556 t
ORIGIN      
        1 tcgcctggcg accgggaaga taagcgggca ggatggctac ctggcagaat cgatcaataa
       61 agatatcgaa gagtgcaacg ccatcattga gcagtttatc gactacctgc gcaccgggca
      121 ggagatgccg atggaaatgg cggatctcaa cgcagtactc ggtgaggtga ttgctgccga
      181 aagtggctat gagcgggaaa ttgaaaccgc gctttacccc ggcagcattg aggtgaaaat
      241 gcacccgctg tcgatcaaac gcgcggtggc gaatatggtg gtcaacgccg cccgttacgg
      301 caatggctgg atcaaagtca gcagcggcac ggagccgaat cgcgcctggt tccaggtaga
      361 agacgacggt ccgggaattg cgccggaaca acgtaagcac ctgttccagc cgtttgttcg
      421 cggcgacagt gcacgcacca ttagcggcac gggattaggg ctggcgattg tgcagcgtat
      481 cgtggataac cataacggga tgctggagct tggcaccagc gagcggggcg ggctttccat
      541 tcgcgcctgg ctgcctgtac cggtaacgcg gacgcagggc acgacaaaag aagggtaaaa
      601 aaacgggagg cgaaggtgcc tcccgttttt agattgtttt caattgattg ataatgaaag
      661 gattcgcttg caaaagcagg agcaattttc gaggtgctcc ggtaggttga cggcgtttgg
      721 tttcccagct tttgaccagg tcataactta cccctacggc gacagcaaaa tcttgctgac
      781 gtaaacccgt tgcttcacgg atcgctttta cattgatctc gctaaaagta tggacgtgct
      841 ctggttcagg cgtttcttca cccttttcga tgcgtaccat ttcttctgcg ctggcaagaa
      901 gatcagcaaa taattcgtct ttcatctgtt ttcaatgcct ccgttggcat ttcggccatg
      961 gtggaatttg tggagcttca acattaaagc agcttctcgg acaatagttt caatgccttt
     1021 ctttgttctt ctgttaaatc atcctgttca tttttaggat aaatcaacgc cagataaatt
     1081 cggccattgc gtgttacgtt gtaataaatt atccgaatgc caccccgttt ccccattcca
     1141 gaacggttcc agcgaatttt tctaaaactg cctgtacctg cgataatgtc accggctgtg
     1201 ggatcttgaa tgagtagttc ctgaaaagcc cggaactcat catcaggtaa caataactgg
     1261 cggcgtttgc taaatccttg tagttcaata aacgtgaaca taatgcatcc tgcggtctat
     1321 tgataattat aagtgtaccg tgtacatatt ggtagctaat tagatataaa aaaacccctt
     1381 atccgtttgg ataaggggtc aatgagatat cagctgatta cagtttcgga cctgcttcta
     1441 ccagcgcggc acccgctggg gtgtcagtgt atttatcgaa gttgtcgata aacagtttcg
     1501 ccagcgtttc agctttttcc tgccactgtt ccggggaagc atatgtgtta cgcggatcaa
     1561 ggatcttggt gtctacgccc ggcagttctg ttggaatcgc caggttaaac atcggcaaag
     1621 tgaaggtttc tgcgttatcc agcgaaccgt taaggatggc gtcgataata gcgcgggtat
     1681 ctttaatgga gatacgtttg ccagtaccgt tccagccggt gttaaccaga tacgcctgcg
     1741 cacccgctgc ctgcatacgt ttcaccagca cttctgcgta ctgagtcggg tgcagcgaca
     1801 ggaacgccgc gccgaagcaa gcggagaaag ttggcgtcgg ttcggtgatg ccgcgctctg
     1861 taccagccag tttggcggtg aagccagaga ggaagtggta ctgggtttga tcggcagtca
     1921 gacgagaaac cggcggcagc acaccaaagg cgtctgcggt caggaagata actttagttg
     1981 cgtggcccgc tttggaaacc ggcttaacga tgttatcgat gtgatagatc ggataagaaa
     2041 cgcgggtgtt ctcggttttt gaaccatcat caaagtcgat tgtgccatct tcacgcacgg
     2101 tgacgttttc cagcagcgca tcgcgacgga tagcgttgta gatttcaggt tccgcttctt
     2161 tcgacagctt gatagttttt gcgtagcagc cgccttcgaa gttaaacacg ccgtcatcgt
     2221 cccagccgtg ctcatcatcg ccaatcaggc gacgtttcgg gtcggtggaa agggtggttt
     2281 taccggtgcc ggaaaggccg aagaacaccg caacatcgcc tttctcacca acgttggcgg
     2341 agcagtgcat agaagcgata cctttcagcg gcagcaggta gttcatcatt gagaacatcc
     2401 ctttcttcat ttcgccgccg taccaggtgc caccaatcag ctgcatgcgc tcggtcaggt
     2461 taaacgccac gaagttttca gagttcatcc cctgttcttt ccactggggg ttagtgcatt
     2521 ttgcgccgtt cataacgata aagtctggtt tgaaacctgc cagctcttca tcgctcgggc
     2581 gaataaacat gtttttgaca aaatgcgcct gccaggccac ttcggtgatg aaacggacgg
     2641 aaagacgagt atccgggttc gcaccacaga aggcgtcgac aacgaacaga cgtttgccgg
     2701 aaagctgcct ggtcaccaga cctttcagat gctgccaggt ttccggagag agaggtttgt
     2761 tgtcgttctt acctttgcct ttgtctgccc accagaaagt atcgcgagtg gtatcgtcac
     2821 gaacgatata cttatctttc ggtgaacgac cggtgaagat cccggtatcg acggcaacgg
     2881 cacccagatt agttaacacc ccgcgctcat aaccggtcag gctcgggtcc agctcttcct
     2941 gatacagcag atcgtagctt gggttgtaaa cgatatcatg tacgtcactg ataccataag
     3001 cctcgagttc ttgcggggtc aaaccattgt taacgcgcat ttcactgctc cttagccaat
     3061 atgtattgcc tgaatagtaa agtctttttg ggggtgttaa ccgcgacaag gctcatagat
     3121 ttacgtatct ggagaaattc atatttgaca aaaaactccg tgattcctgt cacgaaacgg
     3181 ttgctattat cgcagaaata gcattttagg tgtggaaaat gttctcaaaa ggttaaatct
     3241 tggtttatat ggcgggaata ttgtgagtgg aatcgcattc atgaatgaaa ttgatgcaaa
     3301 ttcaatcggt aaagtatcga ttcaccacgc taactttttt tgattatttt ctgaaaaaag
     3361 caaaatacta ctgcccggag gcgataatta acccaccttt tgattctgga taaccaacat
     3421 ggataacgtt gaactttcac cggcaacacg ctgggggatg attgctaccg gattacttca
     3481 ggggctggtt tgctacctgc tgatcgcctg gcttgccgga aaaaatcaca gttggattgt
     3541 ttatggcgtg cctgcgaccg tcgcgttttc atccgtttta ctcttctccg tgatctcctt
     3601 taaacagaag cgcctttggg gatggctggc gctggtgttt attgccacgc tgggaatgag
     3661 cggttggcta aagtggcaaa ttgatggcat gaaccactgg agagcagaaa aggcgctttg
     3721 ggattttggc tgctatctgc tgctgatggc aatgttgttg ctaccgtgga tacaacaaag
     3781 ccttcgcatc cgcaatgaca gtagccgcta tcgctatttt taccagtcag tatggcataa
     3841 cttacttata ttactggtga tttttctcgc caatggcctg acgtggctgg tacttttact
     3901 gtggggagag ttgtttaaac ttgttggcat cacgtttttc aaaacgctct tttttgcaac
     3961 cgactggttt atttatctca cgttaggtct ggttaccgca ctggcggtga tcctcgcgcg
     4021 tacacagtca cgtttaatcg actctattca aaagttgttc acgttaatcg ccacggggtt
     4081 gctgccgtta gtatcattgc taaccctgat gtttattatc accctgccgt ttacgggcct
     4141 gagcgcgatt tctcgccaca tttccgccgc cgggttgctg ttgacgctgg cctttttgca
     4201 attgatctta atggctattg tccgcgatcc gcaaaaagcg tcacttccct ggacggggcc
     4261 attgcgttgc ctgattaaaa ccgctttgct ggttgctccg ctgtatgtgt tcgtcgccgc
     4321 ctgggcgtta tggctgcggg tcgctcagta cggctggact gccgaccgct tgcagggcgt
     4381 gctggcggtg ctggtgttac tggtatggtc gctggggtat tttgtcagta tcgtctggcg
     4441 taaagggcaa aatccccttg atcttcaggg caaagtgaac cttgcggttt cgttattggt
     4501 gttggtaata gtggtgcttc ttaattcgcc ggtgctggac agtatgcgca ttagcgtgaa
     4561 cagtcatatg gcgcgttatc agagcggcaa aaacacgcca gaccaggtaa ctatctacat
     4621 gctcgaacag agcggtcgct atggacgtgc ggcgcttgag tcgctgaaaa gcgatgccga
     4681 gtatatgaaa gacccgaaac gcgcgcggga tctgctgatg gcgttagatg gagagcaaca
     4741 tcttcaggaa caggtatcgg aaaaagtatt agccgataat gtgttaattg ccccaggttc
     4801 tggtaaaccg gatgcgacat tctggtcggc cttaatccag gatcgctata acgtgatgac
     4861 ctgtattgaa aaagacgcct gcgtactggt cgagcaagat ctgaatagtg atggtcaggc
     4921 ggagcggatc ctgtttgctt ttaatgatga cagagtcatt gtctatggct ttgactcagc
     4981 cagaaaagaa tgggacgcgc ttgatatgag tttacttccg cgccaaataa cgaaagaaaa
     5041 attactcgct gctgcgaagg atggaaagct ggggacgagg cctaaagcgt ggcgcgatct
     5101 tgtagtggat ggtgaaaggc tggatgtgaa tctgaatgag tgacaaagcg catcaggtgt
     5161 attagtcgta aaatccggca gagtcctctc tgccggacat actcattaat gaacttgcgg
     5221 atctgccgga gacgcgttgt tgcggatctc ggcaatatcc atcgcattga acagatagtg
     5281 gttaccgcag taatcacaat gcatgtcaat ttcgccatct tccgccagga tgctatcgac
     5341 ttcttcatca ggcagcgttt tcagcgcatc ggcgcaacgt tcacgcgagc aggtgcattt
     5401 gaactccaca tcctgcggat cgtaaaccgt cacctcttct tcgtgataca aacgccacaa
     5461 cacttcgttt gccggtaagg tcagcagttc ttcggttttg atggtttcgg ttagcgttgc
     5521 caggtggtca aagtcgtcct gctgggcatt ttgcgcaggc attacctnnn nnnnnnnaac
     5581 aacattccgc ctgcagccgg tttgccgtct acgtcgccgg tgcgaataaa caggcgcgtc
     5641 ggcagctgtt cagaacgcat aaagtaatct tccaggcagg ccgccagggt atcaccttcc
     5701 agaccaacca cgccctgata gcgttcgcct tcgctcgggg tgatggtgat caccacgtaa
     5761 ccattgccga ccagcgtttt caggtcggca ttttctggaa tttcgccctg cacgcgcgcc
     5821 acaccgcgca tctgctggtt gttgttaccg ttaataaccg ccagattcat cggaccgtcg
     5881 ccttgcagct gtacggtgat atc
//
LOCUS       DAWDRC010000205         5879 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6190_length_5879_cov_2.295501, whole genome shotgun sequence.
ACCESSION   DAWDRC010000205 DAWDRC010000000
VERSION     DAWDRC010000205.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6190_length_5879_cov_2.295501"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    354..363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1518..1527
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3125..3134
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5152..5161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5503..5512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1425 a         1426 c         1486 g         1492 t
ORIGIN      
        1 ggctgaaagc gtggtgcaat gtctggtgct ggctgggtta attattcctg tcgcgcgact
       61 gcgcccggtg ctgacgctca aaaacagtgg agggaaaaaa ggatgatctt gctacaactc
      121 tcctctgctc aggggccgga agaatgttgt ctcgcagtga aaaaagcact ggacaggctg
      181 attaaagaag ctacccgaca ggacgtcgcg gtaacggtgc tggaaacaga aacgggtcgc
      241 tactctgaca cactgcgttc ggcgctgatt tctctggatg gcgataacgc atgggcttta
      301 agcgaaagct ggtgcggcac tatccagtgg atttgtccga gtccgtatcg gccnnnnnnn
      361 nnntgatgag caggaacaat cggatgcaat ccgttatgag acgctgcgtt cgtcagggcc
      421 gggcggtcaa catgtcaata aaaccgactc ggcggtacgc gccacgcatc tgtcatccgg
      481 tattagcgtg aaggttcagt cagagcgcag tcagcatgct aacaaacggc tggcgcgatt
      541 actgattgcc tggaagctgg aacaacagca acaggaaaat agcgcggcgc tgaaatcgca
      601 gcggcgaatg ttccatcacc agattgaacg aggcaacccg cgacgaacgt tcacagggat
      661 ggcttttatc gaaggataat gaaggatgaa actgccggaa ggcgattaaa cgccatccgg
      721 cagcaaatca aataattact tcgccggaat ttctttcagc agttcagtca gcagtgtcca
      781 gtaatggcca acgctttcga tatgaacttg ctcatccgga gagtgtggac cggtgatggt
      841 tggcccgata gacaccatgt ccatttccgg atacggtttt ttgaacagac cacattccag
      901 gcccgcgtgg ataatctgga tgttcggcgt tttgttgaac agacgctgat aggtttcacg
      961 taccagatgc attaccggag agttagcgtc cggctgccag ccaggatatg cgccttttgc
     1021 ttctgttttc gcacctgcca gtttacccag tgaatccagc atgctcacta cgtagtcttt
     1081 accgctgtcg atcagagaac ggatcaggca gtgaatttct acgttattgt cggtcatggt
     1141 caccacaccg acgttcaggg aggtttcgac cacacctttt gccacgtcgg agttgcggat
     1201 cacaccgttc ggggtggcgt tcagcagacg aataaaggta tcgcgagatt tcgccgttaa
     1261 tgcagctttg tcgttcgcta cggagtccag cagcaatgcc agattcttct ctttttctgc
     1321 cagttcgttt ttcaggatct cctgataggt atccaccaga gatttcaggg cgtcgacttt
     1381 atcagctgcg acagcaatgg tcgcaaaggc ttcacgcggg atggcgttac gcagtgtgcc
     1441 gccgttgaaa tcaataaggc gcaggtccag ttcttctgca tgacccgcca ggaagcgcac
     1501 cagcagtttg ttggcatnnn nnnnnnncca acgtggattt caccgccgga gtgaccgcct
     1561 ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct
     1621 aaatgcaggt tggaggtgaa gtcgatacct cccgcacaac ccatgtagat ttcaccttct
     1681 tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg taagccaaaa
     1741 gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc
     1801 acgttttcgt cagccagaac cgccagtgca gaggccatac caatgccgtt atccgcaccc
     1861 agcgtggtgc cgcgtgcttt aacccattcg ccatcaatat aaggctggat aggatctttg
     1921 gtgaagtcat gcacggtgtc gttatttttt tgcggcacca tatcgaggtg ggcctgcaag
     1981 acgaccggtt tacgattttc catacctgcg gtagcaggtt tacgaatcag gatattacct
     2041 acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat gtattcagcg
     2101 agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa aatatcccac
     2161 agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt cgatcacccg
     2221 caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa gacaggcttg
     2281 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     2341 cgcggactca tgtgaaatac tggtttttag tgcgtcagat ctctataatc tcgcgcaacc
     2401 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     2461 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     2521 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc
     2581 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     2641 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     2701 aggcttcatc gttattgatg acctgggaga taccggtggt actgcggttg cgattcgtga
     2761 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     2821 tgatgattat gttgttgata tcccgcaaaa tacctggatc gaacagccgt gggatatggg
     2881 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     2941 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     3001 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     3061 aaacctcgac gctagtccgc cgctttagtc acggcgcaca accgcctgtg cagtcggccc
     3121 ttgannnnnn nnnntggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     3181 acccccgtga aatcctcgat gtccaggcgc gtattgtgat gagcgatgcc gaacgtaccg
     3241 acgatgattt atacgatacg gtgattggct accgcggcgg caactggatt tatgagtggg
     3301 ctacccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg caactcagcg
     3361 gtcgacactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct catctgaaag
     3421 gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg cgcctatgaa gaggccgctc
     3481 agcgtctgcc gggcacgatg cgacagatgg agtttaccgt acccggcggt gcgcccatca
     3541 ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta atgtgtggtg
     3601 gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt gcgccgcgcg
     3661 gcattgctat gctgactatt gatatgccgt cggtgggctt ttcttcaaaa tggaagctca
     3721 cccaggactc cagcctgttg catcagcacg ttttaaaggc gctgcctaac gtaccgtggg
     3781 tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt gccgtgcgtc
     3841 tggcatacct tgaatcgccg cgtctgaaag tggttgcctg tcttggtccg gtagttcata
     3901 ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt gacgttctgg
     3961 ccagtcgttt agggatgcat gatgcttccg atgaagcgtt gcgagtggag ttgaatcgct
     4021 attcattaaa agtgcaagga ttgctgggac gtcgctgtcc aacgccaatg ttatcaggct
     4081 actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacg tcgtcatctg
     4141 ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt gacaaaggcc
     4201 ttcaggaaat cacagactgg atcgaaaaac ggttgtgtta aaaatttgct aaattttacc
     4261 aatttggtaa aacagttgca tcgacgttac cgagtggaca cccgaagagc agattgatca
     4321 aaaaatttac cgcactaggc ccgtatattc gtgaaggtaa gtgcgaagat aatcgattct
     4381 ttttcgattg tctggctgta tgcgtcaacg tgaaaccggc accggaagtg cgtgagttct
     4441 ggggctggtg gatggaactg gaagcgcagg aatcccgttt tacctacagt taccagtttg
     4501 gtctgttcga taaagcaggc gactggacga gtgttcagat aaaagatgcc gaagtggttg
     4561 aacgactgga gcacaccctg cgtgaatttc acgagaagct gcgtgagctg ctggcgacgc
     4621 tgaatctgaa gctggaaccg gcagatgatt ttcgtgatga accggtgaag ttaacggcgt
     4681 gaatgaaatg tgccggatgc atcacatccg gcaatatcca ttaaaactga taggtcatgc
     4741 caaccgcgac aatatcatca ttattaatat tcaatttgtt atcgctatcc agttggttga
     4801 ttttataatc aacaaacgct gacatatttt tgttgaaata atacgtagca ccaacatcga
     4861 cataattgac cagatcttca tcaccgatgc cctcaatatc tttccctttc gataagacat
     4921 aacccagcga tggacgcaga ccaaagtcaa actggtattg agcgaccgct tcaaagttct
     4981 gcgtcttatt ggcaaagccg ccagagattg gcgtcatttt acgtgtttca gaatagaaag
     5041 ttgccagata aatattattg gcatcgtatt tcagacctgt tgcccatgct tctgcacgct
     5101 tgcctgtgcc acggctttgc aggttctgct cgttggtgcg atcagagttg gnnnnnnnnn
     5161 ncactgatgg cgaaatcgct gccaccaaag tcatatgtca atgacgtgcc gaagccatcg
     5221 ccgttttgct ttttaacgtc gcggttttcg tttttccctt gatattgcag ggttaagttc
     5281 agaccatcga taacgccgaa gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt
     5341 ttggtcataa agttgtcggt ctgcgccgag gagtcgccac caaattccgg gaacatatcg
     5401 gtccaggctt ccacgtcata caacgcgccc aggttacgac cataatcgaa agaacccaaa
     5461 tctttatatt tcaacccggc aaaagcgaga cgcgtttttt gcnnnnnnnn nntgtgcagt
     5521 atcactctcc gctttattac cggcaaactc agcttcccaa cgaccataac cagtcagttg
     5581 atcgttaatt tgtgtttcgc ctttaaaacc aaaacggata taactctggt cgccatcttt
     5641 actgtcatta tcactcatat aatgcatggc tttaactttg ccatagacat ccagtttatt
     5701 accgtctttg ttatatattt ctgcggcctg tacggataca gatgccacaa tgcccatcac
     5761 cactaatgcc agagtgctct ttttcatttt cattcctgat tttaattaac gcgcgaatat
     5821 tcagcgggag agtcccgttg aaaacaggaa agtttttaac ctgagattgt taaagatat
//
LOCUS       DAWDRC010000206         5879 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6189_length_5879_cov_2.472356, whole genome shotgun sequence.
ACCESSION   DAWDRC010000206 DAWDRC010000000
VERSION     DAWDRC010000206.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6189_length_5879_cov_2.472356"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1419 a         1602 c         1553 g         1305 t
ORIGIN      
        1 aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccacatta
       61 aaaaagcccg cttcggagag ctttttgctt ttctgcgtat attcaatgcc gaatgcgatg
      121 ttgactcgtc ttatccttca aagtcggatg cgacgctgcc gcgtcttatc cgacctacgg
      181 ttggcatgca tccagcaatg ttgtgaggct acaaccctaa cgcatatttc agcgcctgac
      241 gtttcaacac gccagcacgc tccgccgcca tcaaaccaag attacgcaca aaacgcagcg
      301 gtggcagatt attgctgaat ccggcataaa acagatccat accactttgc atcatgaagt
      361 tatccgccat acgccgcatc tggtaacgct tgagcacagg ataactggcc cacgcttcgc
      421 cgtagctgcg ggcgttcacc agaacgtcaa tcagggcatc gacatcacga taaccaagat
      481 tcactccctg tcccgccagc ggatggatgg tatgcgcggc atcgcccacc agagcaagcc
      541 ctggttgcac atactgcaac gcatggcgac gtatcagcgg aaacgcacca gcggcaagcg
      601 gtgtcacgta acccagacgc gacgggaaat gcttcgcgat ttccgcctgt agctgcgcca
      661 tattcatatt ctgcaactgg cgaatgcgcg ccggagagtc ataccacacc agcgatgccc
      721 agttatcaaa caacggcaga aacgcacgcg gtccgtccgg ggtaaattgc tgccaggtgc
      781 tgtcgccggg atcgttctca cactgtacgc taatcaacat acacgactgc gcatactgcc
      841 aggcatgaac gccaattccc gccatctgtc gcacctgcga atttgcgccg tcggcaccaa
      901 tcacccgctt cgcgaggatc gtttcaccgc ccttcagttc cagctcctga agatcattat
      961 ggcgatgcag tgcaatcagc gaggttggcg cacgtaacgt tactttcgga tgcgcttcca
     1021 gcgcctgcca caacgcctgt tgcaggacag tgttttccac catgtagcca agcaacggta
     1081 gcttaagttc agcggcgtca aacaccacat gcgccgtttc ccactcccac gtttccagtc
     1141 tgcggtaagg atggcaacgc atagcctgta ctgcatccca gacccctaac cctttaagca
     1201 atgataccga agccgcacta atcgccgaga tccgcacgtc cggctggctg tcagcgacaa
     1261 acggtgcagg ttgggcatgt tcgataacca ttaccgaaaa tccgtgctga gccagtccca
     1321 gcgccagtgc gccgccgacc attcctccgc cgacaatggc aatttccgtt ggttgatttg
     1381 tcatggtcaa tcatcctgtt agcaatatgc tttaagttta caggattttt acccccaagg
     1441 gctgataacg ctcatactgg tcacaacggc agcaaagcat tacactatgc gccctgcatt
     1501 cctggctact atttcgcaag agcaagtcga tgaccaaaaa actccatatt aaaacctggg
     1561 gctgtcagat gaacgagtac gattcatcga agatggccga tctgctggat gccacccacg
     1621 gctatcaact gaccgacgtg gcggaagaag cggatgtgct gctgctgaac acctgctcaa
     1681 tccgcgagaa ggctcaggaa aaagtcttcc atcagttggg tcgctggaaa ctgttaaaag
     1741 agaagaatcc agacctgatt atcggcgtcg gcggctgcgt ggcatcgcaa gaaggcgagc
     1801 acattcgcca gcgcgcccac tatgtcgata ttatttttgg gccgcaaacg ctgcaccgtc
     1861 tgccggagat gatcaactcc gtgcgcggcg accacagccc ggttgtagat atcagcttcc
     1921 cggaaatcga gaagtttgac cgtctgcctg aaccgcgcgc cgaagggccg accgcgtttg
     1981 tctccatcat ggaaggctgc aataaatatt gcacctactg cgtagtgcct tacacccgtg
     2041 gtgaagaggt aagccgtccg tccgacgata ttctgtttga gattgcccag cttgctgctc
     2101 agggcgtgcg tgaagttaac ctgctcggcc agaacgtgaa cgcctggcgc ggtgagaact
     2161 acgacggcac caccggatcg tttgccgatc tgctgcgtct ggttgcggcg atcgacggga
     2221 tcgatcgtat tcgctttacc accagccatc cgatcgaatt caccgacgat atcatcgaag
     2281 tgtaccgcga cacgccggag ctggtgagct tcctgcatct gccggtacag agcggttccg
     2341 atcgcattct gaacctgatg ggacgtaccc atacggcgct ggagtacaaa gcgatcatcc
     2401 gtaaactgcg tgcggcgcgc ccggatattc agatcagctc tgacttcatc gtcggcttcc
     2461 ctggcgaaac caccgaagac ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg
     2521 acatgagcta cagctttatc ttctctgcac gtccgggtac accagccgcc gatatggttg
     2581 atgatgttcc ggaagaagag aagaagcagc gtctgtatat tctgcaagag cgcattaatc
     2641 agcaagcgat ggcatggagc cgccggatgc tcggcaccac ccagcgcatt ctggtagaag
     2701 ggacttcacg taagagcatc atggagcttt ccggtcgcac tgaaaataac cgcgtggtta
     2761 acttcgaggg cacgccggat atgatcggta aattcgtcga tgtagaaatt accgacgtct
     2821 atccaaactc cctgcgcgga aaagtggtgc gtactgaaga tgaaatgggt ctgcgcgtgg
     2881 cagaaacacc ggaatcggtg attgcccgta cccgcaaaga aaacgacctt ggcgtgggtt
     2941 attatcagcc gtaattctca ggcccgccgt tccggtgggc cttgtttttt ctctctttat
     3001 ccccatcttt atcgcaatgc ttgcgccttt ttcctgtggc gtgaataatt tccttatagg
     3061 ccggtgaatg ttcagcttcc ggcggcatac agataagagg aacggtttga acatagacac
     3121 tcgcgaaatc accctggagc cagcagacaa tgcgcgtctg ttgagcctgt gcggcccgtt
     3181 tgatgacaac atcaaacaac tcgaacgccg tctcggcatc gaaatcaatc gccgcgataa
     3241 ccactttaaa ctgaccggcc gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag
     3301 cctgtatgtc gatacagccc cgatgcgcgg tcagattcag gatatcgaac cggaacagat
     3361 ccaccttgcg attaaagaag cgcgggtact ggagcaaagc gcggagagcg tgccggagta
     3421 cggcaaagcg gtcaatatca aaaccaaacg cggcgtaatt aagccgcgca cgccaaacca
     3481 ggcgcagtac atcgccaata ttctcgacca tgacattacc ttcggcgttg gcccggcggg
     3541 tacgggtaaa acctacctgg cagtggctgc ggcagttgat gccctggagc gtcaggaaat
     3601 tcgccgtatt ctgctgactc gtccggcggt cgaagccggt gagaaactgg gcttcctgcc
     3661 tggcgattta agccagaaag tagacccgta tctgcgcccg ctgtacgacg cgctgtttga
     3721 aatgctgggc tttgagaaag tcgagaaact gattgagcgc aacgttattg aagtcgcacc
     3781 gctggcctat atgcgtggtc gtacgctgaa cgacgcgttt atcattcttg atgaaagcca
     3841 gaacaccacc atcgaacaga tgaagatgtt cctgacccgt atcggtttta actcaaaagc
     3901 ggttatcacc ggcgacgtca cacagatcga cctgccgcgt aataccaagt ctggtttgcg
     3961 tcatgccatc gaagtgctgg ccgatgtcga agagatcagc tttaacttct tccacagcga
     4021 agacgtggtt cgtcacccgg tggtggcgcg tatcgttaac gcctatgaag cctgggaaga
     4081 agccgaacaa aaacgtaaag cggcgctggc agcagaacgc aagcgcgaag aacaggaaca
     4141 aaaatgagtc aggtgatcct cgatttacaa ctggcatgtg aagataattc cgggttaccg
     4201 gaagagagcc agtttcagac atggctgaat gcggtgatcc cgcagtttca ggaagaatcg
     4261 gaagtgacta ttcgcgtggt cgataccgcc gaaagccaca gtctgaatct gacctatcgc
     4321 ggtaaggata agccgaccaa cgtgctctcc ttcccgtttg aagtaccgcc tggcatggaa
     4381 atgtcgctac tgggcgatct ggttatctgc cgtcaggtgg ttgagaagga agctcaggag
     4441 caaggcaaac cactggaggc gcactgggcg catatggtgg tgcacggcag tctgcatttg
     4501 ttaggttacg atcacatcga agatgacgaa gcagaagaaa tggaagccct cgaaacagag
     4561 attatgcttg ctctgggcta tgaggatccg tacattgccg agaaagaata atttgccagg
     4621 catttgactg gcagacgtcc ccgccgttgg tgattaacga cggcgttttg attaactaac
     4681 atgagaaccc atacgacgcc atgagcgacg acaattcaca cagtagtgac acgataagca
     4741 acaagaaggg atttttctcc ctgttactca gccaactttt ccacggtgaa ccgaaaaacc
     4801 gtgacgaact gctggcgctg atccgtgatt ccgggcagaa cgaccttatc gacgaagata
     4861 cgcgcgatat gctcgaaggg gtgatggaca tcgcagacca acgcgttcgc gacatcatga
     4921 tcccccgctc ccagatgatt accctgaaac gcaaccagac gctggacgaa tgccttgatg
     4981 tcatcattga gtccgcccac tcacgtttcc cggtgattag cgaagacaaa gatcacattg
     5041 aagggattct gatggcgaaa gacttgctgc cgtttatgcg cagcgatgct gaagccttca
     5101 gcatggacaa agtgttacgt caggcggttg tcgttcctga aagtaagcgc gtagaccgga
     5161 tgctgaaaga gtttcgctct cagcgttacc acatggcgat cgtcattgac gaattcggtg
     5221 gggtttccgg tctggtgacc attgaagaca tcctggaact gattgttggt gagattgaag
     5281 acgagtatga cgaagaagat gatatcgact tccgtcagct gagtcgtcat acctggaccg
     5341 tgcgcgcact ggcttctatt gaagacttca acgaagcgtt cggcacccac tttagcgatg
     5401 aagaggttga cactatcggt ggtctggtga tgcaggcatt tgggcatctt ccggcgcgtg
     5461 gcgaaaccat cgacatcgac ggttaccagt tcaaagtggc gatggccgac agtcggcgta
     5521 ttattcaggt tcatgtcaaa atcccggatg actcacccca gccgaagctg gatgaataaa
     5581 accgaaactg gatagataac tacatggctt ttgcctcatt aattgaacgc cagcgcattc
     5641 gcctgctgct ggcgttatta ttcggtgcct gcggaacgct ggccttctct ccttacgacg
     5701 tctggcctgc ggcgattatt tcgctgatgg ggcttcaggc gttgaccttt aaccgccgtc
     5761 cactccagtc tgccgctatt ggcttttgct ggggatttgg cctctttggc agcggtatta
     5821 actgggtcta tgtcagcatc gcgacctttg gcggaatgcc tggcccggtt aacatcttc
//
LOCUS       DAWDRC010000207         5836 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6246_length_5836_cov_2.103096, whole genome shotgun sequence.
ACCESSION   DAWDRC010000207 DAWDRC010000000
VERSION     DAWDRC010000207.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6246_length_5836_cov_2.103096"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4302..4311
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1500 a         1557 c         1400 g         1369 t
ORIGIN      
        1 gataagacgc gttaagcgtc gcatcaggca acaaactcac gggtgagcca caaaccggga
       61 aagccgctgg cgcagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt
      121 cagcgccacc gcgatccccg gccagtccag agccagttca tgacgcaggc gtaccgcgcg
      181 ttgcaccaca atggcggcat ggtcgtcaaa tacccaggtt gtctcctgga tctcactcgg
      241 ttcaaccacc cccaaaccga caatttcatt caactcctct tcagagatgc cggtatgcag
      301 gcaaaattcg gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct
      361 ttacgtggat caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt
      421 tcatccggtt tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt
      481 ttgctcacca gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gccaggcggg
      541 atagtcagca aaatgctttc tttcagtgtt ggaacggtga ctttagtacc cagcgccgct
      601 tcccacgggc taaccggcac cactatttcc agatcctggc cgacaatatc aaacagcgga
      661 tgtggcgcaa tgtgaatcac cagccacagg tcgccatttg gaccgccgtt ttcgcccggc
      721 gtcccctggc ctttcaggcg gatgcgttga ccattgccga cgcccgccgg gatcttcaca
      781 ttcagcgttt tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta
      841 tagctgatgg tacgcttatg ctcagtaagc gtttcttcga ggaaaaccgc cacttcgatt
      901 tcaatatcgt ggccgcgtgt ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa
      961 attgacgaga agatatcgtc aaaatcttcg gcgttaaaac tctgaccgtc gccatgatgg
     1021 aactgacggt taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga
     1081 cgttgttcat cacttaacac ttcccaggct tcggcgacct ctttgaagcg ggcttcggca
     1141 tccggttctt tgctgacatc aggatggtat ttgcgggcca gtcgacgata ggcggtcttg
     1201 attgtcttta gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc
     1261 atagcgttat ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat
     1321 aagtgtaggg taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc
     1381 gcggagggga ggtcgcctca aggtgggagg aacatatttt atttacctcc ctcctgaact
     1441 tttggctcat gcttacaagt taaacaataa ggaaaggaat gcacaactcg actatttaaa
     1501 tcgttatgca atttaatgat aaagacatgt gctaaaaacc attgctatta gtctcacatt
     1561 ctttttttat tggtaatggt aacgccgctg attttcttta tagctactga tgtaatcatt
     1621 agcggcgtaa acgaggccgg ggctaatcag gcatagtttg cgtcaaaact tgcctgtttt
     1681 taaaggggaa tatagaaaaa caggcgttca acgagccatt ttgcgaacct gttcccggaa
     1741 aaatgacata tttctgtcac actctttaat tattgataac aaaagaggtg tgaggaatga
     1801 acaaaacgct aatcgccgca actgtggcag ggatagtttt actcgcttca aatgctcagg
     1861 cacaaaccgt accggaaggc tatcagctac agcaagtgct catgatgagc cgccataact
     1921 tacgtgcgcc gctggcgaac aatggcagtg tgctggagca gtcgacgccg aataaatggc
     1981 ctgaatggga cgtccccggt gggcagctca ccactaaagg gggcgtgctc gaagtgtata
     2041 tgggccatta tatgcgtgaa tggctggcag aacaggggat ggtgaaatca ggggaatgcc
     2101 cgccaccgga caccgtttat gcctatgcca atagtctgca acgcaccgtc gcgaccgcgc
     2161 agttctttat tactggcgca ttccctgggt gtgatattcc tgttcatcac caggaaaaaa
     2221 tgggcactat ggaccccacc tttaacccag tgatcaccga tgattccgcc gcattcagtg
     2281 aacaggcggt ggcggcaatg gagaaagagc tcagcaaact ccagcttacc gacagctaca
     2341 agctactgga acaaatcgtt aactataaag attctcctgc gtgtaaagag aagcaacaat
     2401 gttcgcttgt agatggcaaa aataccttta gcgcgaagta tcaacaagaa ccgggcgttt
     2461 ccgggccgct gaaagtcggc aactcgctgg tagatgcgtt tactttgcaa tactacgaag
     2521 gttttccgat ggatcaggtg gcctggggag aaatcaaatc tgaccagcag tggaaggtgt
     2581 tgtcgaagct gaaaaacggt taccaggaca gcctgtttac ctcaccggaa gtggcgcgca
     2641 atgttgcgaa accgctggtc agttatatcg acaaagccct ggtcaccgat cgcaccagtg
     2701 ctccgaaaat cacggtatta gttgggcacg actccaacat tgcctctctg ttaacggcgc
     2761 tggatttcaa accgtatcag ttgcatgacc agaacgaacg cacgccgatt ggcggcaaaa
     2821 tcgttttcca gcgttggcat gacagcaaag ccaatcgcga cctgatgaaa attgaatatg
     2881 tgtaccagag tgcggaacag ttacgtaatg ccgatgcgtt aaccctgcag gcacctgcgc
     2941 agcgcgtgac gctggaatta agcggttgcc cgatagatgc caacggtttt tgcccaatgg
     3001 ataagtttga tagcgtattg aatgaagcgg tgaaataaca gaaaactccc ccgcgtgaag
     3061 cgggggagtc gctggttaaa cgtttttacg ttcgatggtc tgttcgcccc aaaaaagcga
     3121 atctttatcg gtcttagcaa aggctttgac taacacttca tcgctacctt cttgccaaat
     3181 cttttccgcc attttttcgt cgtacccggc gacttcgaaa atggcctcgg ctatttccgg
     3241 cgacgtattg cgcagagatg cccattcacc gacgtgatga gctttcgctt cttgagttgg
     3301 catgcgtatc ctcctgttga agattagccg ttaagtttaa ctgccagacc tgcgacatat
     3361 tccccttgat aacgagcaat agacagttct tcctggctgg gctggcgtga accgtcaccg
     3421 cctgcgatgg tggttgcgcc gtacggcgta ccgccgcgaa cctgtgaaac gtcaaataat
     3481 tcctgcgctg cgtagccaat ggggacaatc accatgccgt gatgcgcaag ggtcgtccag
     3541 gtggatgtga tggtttgttc ctgaccgccg ccagtaccgg tggaactaaa gacgctcgcc
     3601 agttttccgt atagtgcgcc ggaagcccac aggccgcccg tctggtcgag gaaggtacgc
     3661 atttgaccgg acatgttacc aaagcgggta ggtgtaccaa aaataatggc gtcgtaatcg
     3721 gccagttctt gcggggttgc aaccggtgca gtttgcgttt taccgcctgc tttttcaaat
     3781 aattgcggcg gcatggtttc cggtacacgc ttaacgacaa cttctgcgcc atccactttg
     3841 cttgcaccct cagcaactgc gcgtgccatc gtttcaatat gtccgtacat ggaataataa
     3901 agcaccagaa ctttagccat ttctaaccac tcctcgtgtt atctctattc cgtagcgatt
     3961 cgctaccact tatttaaaga taagacgtcc ttttcagagt gcaaatttca caaccactta
     4021 tttgatttat aacaactttc acaagcacgt aattttgtcg caaaatgaca catttttatc
     4081 tcatcgcgtt tttttaatca taagagcggc ttatggataa ttattggaga tgatatctat
     4141 tctcgctaag aagctgttgc aggatattac caaacgcggg tctgcccgcg tcagttcact
     4201 aagcttagtc ccacgtagcg aaaatatggc agccgccata cgccgcgtta attctatgca
     4261 atatgatgtc tatacccaga ccgcggccat taattgcaag cnnnnnnnnn naggtaaaaa
     4321 aggtggcaag agcagtcacg gtaaaagcga caactagccg ggctaatcaa tgacgcatgc
     4381 atttttgtct gtagctcgtc aaaacgccat caccgccggt taccccggcg gttttctgtt
     4441 tatggctcct gatgaacaac ttctggcggt ggaacgtcaa ccaaccttct gcttaacaac
     4501 gcattgagta aaatcgcgcc aaaggtggct gtaccgatcc ctcccaacgt aaaaccgccc
     4561 agtgtgagag caaaatcacc cgcacccagc actaaggtta ctgcgaccat aatcaaatta
     4621 ccgttctggc ttaaatccac actgttttgt acccagatcc gtgcgcctgc gacggcaatc
     4681 agcccgaaca caacaattga tgcaccacca atcaccgcag ccggaatagt atggatcagc
     4741 gcgccaaatt tcggcgaaaa gcccaacagc atggcgatga cggcagcggc aacaaacacc
     4801 agcgtcgagt agacttttgt cacggccatc acaccgatat tctcggcgta ggtggttacg
     4861 ccgctaccgc cgacagagcc agaaagcatc gtcgtcagcc catcgcctac gaatgcccgc
     4921 cccatatacg ggtccatatt gcgtccggtc atcccggcga ccgccttgag atgacctaag
     4981 ttttccgcca ccagaatcac cgccacgggc gcaatcagca tcattgcctg accattaaaa
     5041 gcaggagtgg aaaaatgtgg cagaccgaac caggcagcat ggctgacgag agtaaaatcg
     5101 acggcttttc ccagccctaa aacgttggtc atcacgccat acagcagaca ggcgacaatt
     5161 aatcctacga gaatcaataa tcgctggatc atgccacggg taaatacggc caccagccca
     5221 atacatagca ccgtcattac cgccatccag ctatcaaagg ccgaagccga tacatttttc
     5281 actgcgatag gcgctaagtt caggccaatc gccatcacta ccgcacccgt cacaaccggc
     5341 ggcattagtc gttcaatcca gcgcgtaccg attttcatca ccaccaggcc aatgacggta
     5401 taaaccagcc cacaggcgat aatcccgccc agcgcgatgc tgatattcgg attaattccc
     5461 tgaccattaa agcccgtcgc ggcaatcacc acgccgacaa aagccgcgct ggaaccgaga
     5521 taactgggca cgcgcccgcc ggtaataaag aaaaacagta acgtgccgat ccccgacatt
     5581 aaaatggaaa gattgggatc cagccccatc agaattggca ttaacaccgt cgcgccaaac
     5641 atcgccaccg cgtgttgaac gcccattact gccgtttgag caaacggcaa tcgttcatcc
     5701 ggcgcgacca cgccgctctc tgtagaggtc gattttaact gccagtgagg aaaaccgaac
     5761 attgccatca gctgtctcct taaggaggtt aacaagcagg gcgcatcagc gcgtgataac
     5821 tgcgatcgaa ccacac
//
LOCUS       DAWDRC010000208         5806 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6269_length_5806_cov_2.109720, whole genome shotgun sequence.
ACCESSION   DAWDRC010000208 DAWDRC010000000
VERSION     DAWDRC010000208.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6269_length_5806_cov_2.109720"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2628..2637
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3100..3109
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3744..3753
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1292 a         1712 c         1479 g         1293 t
ORIGIN      
        1 atcctatatg ttttgaccta tcaattgtta ctcaatgatt tattacaact attaataatc
       61 ataatgtgtc aaaacactta agcatcttta cagtcttaga aaggtgtcga ttaacgaagg
      121 ctacatggtg aaaaacaggt tccttcagtg ttaccgaata ttccgtggtg gtctggaggg
      181 agataattct tgctacatcg aaactcagcc cggtgagcgg gtttgacagt tccgcactca
      241 ccattgggct aagggttatc aggtggggtt aaggaaatgg caatacctac ccccgtccaa
      301 actccagtcg ctgcacattc accatcccag gcttctcagc agcactgaca tcaatttgtg
      361 tcacccgcag cgcatatttt tcatccagcg cgtttaacca gttcagcaga tcattaaata
      421 ccacaggatc tatccagacc tggatattct ccccgcgctc ggctattcgc ttaatgacca
      481 ctgaatgcgc cgaagcactg tcgctgatga cccgcgatac ctgtgctggc gttgtcgtgc
      541 cggattttcg cgccgcaata atatccggcg cggcgctctt cagtcgcgcg ttcatcgcca
      601 ccagctgctg caacatcgtc tcctgttgct caatccgttc gctcaacggc tgccagatga
      661 gaacgtaata tccggcgcta aacaggaaca ctaccgctgc cagtaacatg cctttttcac
      721 gcggagaacg ccccgccagg tgttgtgtca gccagtgttc gccacggctt aactggcgtt
      781 cacgccattg ctgaaaatag tgaataaatt tatcgcgtaa catgttattt cctccgcaac
      841 gttacgccgc cggaaaccgc atcaccctct ttctgtaacg cgtcctgttg cacaacataa
      901 tctaccgcca gtgcgctacg cagtttgtcg aagctggcaa agttcgcagc ccgtagctgg
      961 aggtgaagcg tctggcgttt ttgatcaaag gtgaaaccac gcatctcaat gtcggaaagt
     1021 aatgcggatt tcagggtact ggcgatcgca gacaactcgg caagcagatc ggtatcatca
     1081 gcctgtgggc gatatttttt cagcgccatc gccacctgcg agcgtaaatt cacaatccgc
     1141 ttctgctccg ggaatagcgt taagaactgt ttctccgcct gggtgcggct ttgcgccacc
     1201 tgttcgctga cgctccataa cgtcacgccc cgttccacta ccagcgcaac cagaatcagc
     1261 aatatcggca gaatcatcac ccgccagcgc gcccactgtt ttcggtagct gacacgaggc
     1321 tgccacggcc ctgttagcag gttcccttcc ggttcgccat aagtggtaat ggcgggcaga
     1381 gccgtaacgg tcaggcgttc ggcgtccgca ccagcccatg cagacagttc ttccggtgca
     1441 atgccgacca cagttagtga aatcggtaaa ttctgctcat tgagctgtgc gcggaacatg
     1501 accggagcca gcgcccgccc ggcgctccat ccccggcatt catcgatgcg gcagataacc
     1561 cgttgcgcat cgccagccat aaacccacaa ggaatagaca tccagtccgg cgcgacggta
     1621 gcgcgggtga tgccgtttgc ctgcaaccac tgcgcaatgt tgcgcatatg ctgctggtga
     1681 atcacagcta cggttgccag ttgctggtcg attttcaacg gggcgaaatg cagttcatcg
     1741 atatcctggt tcagctcttc ttccagcaag gcgggcagaa tcgtcggtat ctgcttgcgg
     1801 ggcacatcag gcagttcaac ctgccagacg ctgatccatt cgccgggaat gtagagtcga
     1861 atcgcatcag tttgcagcca ttgctggaga cgttcgtcag caacgtcagg ccagataccg
     1921 tgctccacgt cgggtgtatg gcgctgccaa cggatgggat cagcggcgca aagcgggaaa
     1981 aaaatctcaa gcatggaact cactcacttt ctcctgtctg atgccagaga acagaaaagt
     2041 gttgtgggcc catgcggaca attaacgaat tcatcgtcag ttcaatctca ttcacggtga
     2101 tatctgaacg cagccagaag taattgctgt ccacgctcag gatggttttt agctgttttt
     2161 tagtacgctc atcgacgtct gcaagtagcg gctgagcaag aaactgatcg acatcttccc
     2221 agcccttcgc cggacgttgt tgtaataacg cccgcgcctg aacagggctt aaccacgggt
     2281 caaacagcgc ctcaagaatc acactttgcg tgacatctaa tgtattgatg ttgatttgct
     2341 ggcgggccat cggcagcgca cagaccaacg gtttcagttt ttgataaagc ccggcgtcca
     2401 ttccctgcac cacgcgcatc tcgctgatat cagccagcgg ttgattagcg gcgtagaacg
     2461 gcaccgagcg ggcgagatac tcgctgtctt cacgacccag acgcgtctgc acgctgcggt
     2521 cttcgtcaat aaactcccac aggctttcgg ctatcagttc ggcccgataa gcaggcacat
     2581 cgaggcgcga gatcagggca atcagttgtt gtaccgcgag cggacgcnnn nnnnnnntcg
     2641 gctgagcgag ggcattcagg ttaaagcagg cctgtgcgtc acgcagagtg acggcaattt
     2701 gcccctgcgg cagtgggaaa aaacgcgggc cagaagccca cggctgtgcc agatgcacac
     2761 gcttttcatt tttcaggctc agactgagcg cgctcagcgc caggctttct gcactggcgc
     2821 tgtaccacag cgcctgctgg tactcctgct ggctgcgcgt tcgcccaagt tgttgctgca
     2881 tccgactgga aagcgtgatg gttaccagca ttatgaccgc cagcaatacc agcaccacga
     2941 ccagtgccat tccgcgtttt ggtggtgagg tgatcatgat aattgcggcc cgcgtaacaa
     3001 ccagatgcgt tcaatttcgc cccattgtgg cgaatgcagg gttatgcgta ctgccacagg
     3061 aatcgcctgc actgatgacc aactcgcctg ccagcccgtn nnnnnnnnnt aaccgttcga
     3121 gataaccacc atgaatgcgg taaccgacgg tgagcagatt actgcgcggc agacgcatca
     3181 acggattaac cacgccgcca cgtacaaaac gcatcccttc actctcagac gccagcacgc
     3241 cagcgcccgc cagtaacgct ggttcacgct ggccctgatc gcctcttacc ggacgcggca
     3301 tcatttgtgt cagatcgtgg ttcaaaaaac tcatcgtttg ctgcatgagg tttagttttt
     3361 gatcgtgtcc ggcgacggcg ctattcacgc gtgtaacgcc gtttgtcacc tgctgcgcca
     3421 tcagtgccag tgaggcaaaa atggctatcg ccaccagcat ttccagtaac gtgaaaccag
     3481 cgcgagccct tctcactgtt ggcctcccac ggcgctaaac caggcgcgtc gtgactgaat
     3541 cactggcgaa aagtcgtcat gaagactgac ttcaatatcc accgcctgga gcagcacgtt
     3601 accggtattc agcggtgtgg ttcgccagta ccaacggctt cctgccatat cgctctcgcc
     3661 ctggtgcctg accgcccgtt gttcgcgggt catcaggtag agtagagcgg tctggttttc
     3721 agctacccag cccgctaccg tttnnnnnnn nnnctcctca agaatctgct gattactcag
     3781 cgcaccgctg gcaatctgta acaccgccgt ggcagccagc gcaaaaatcg ccagcgcgag
     3841 catcacttcc agcaaggtaa atccgcgctt catggcatcc cctcatcaca ttgattgaga
     3901 gataacgcgc catcgtgcgc cagctttacc gcccaacacg cgttttgtgc cgacgcgtaa
     3961 aaacgcagtg taaacggcgt gacaggttca aaaggcgaaa aacggatctg cggtgtcttc
     4021 tttttcgcct ctttttgcag ctccagttca atatcatgca gcgtcaggcg gcgacgttgc
     4081 atctccagcg catactcctt ctgccagata tcactgccgg gttgtaacag catctgcacc
     4141 tgtttcggca ccgtaacctg tgccgataac cgggtcgcag aaacgggcaa ccattgccct
     4201 tgacgacgct gcataaactg gtaacctggc gggtcgatta gcacaccgag tgtttgccct
     4261 tcgatcactg ccctgtcctt aaactgcgca aagcgagtca gaaaagcctg cgccgctttt
     4321 ttcgcaggcg gctctgaagc ggtcgcaaac gtctgtacca cgcccgcact ggcaaggccg
     4381 ataaggaaaa tcaccagcat gatttccaga agtgtgaatc cgcgttcagg cactgattac
     4441 tgaaactcct gcaaattcca gttaccgata tctgcgccag cgccctcccc gttttcctga
     4501 ccatctgccc ccagggtata aacatcaaac agcccttttt cacccgggct gagatactga
     4561 taatcattgc cccacggatc ctttggcagt cgtttgatgt atccaccggt acggtagttg
     4621 cgggcgtccg ccatattggc cggttgctgg atcagcgcct caagcccctg ctcagtggtc
     4681 ggataacgcc cgttatccag tcggtacata tccagcgcat tctccagcgc cacgatatcg
     4741 ctgatggctt tttgccgatc ggctttctct ttgttgccca acaggttagg caccaccaga
     4801 cttgccagga cgccaagaat aacaatcacc accatcactt ccagcagggt aaaacctgcc
     4861 cgtggttttt gtgtgcggga taacgaattc atgtgataac tccgtaaatt acattccaac
     4921 catattgttc agttgcagca tcggttcgag gatggcgatg acgataaaca gcaccacacc
     4981 cgccatcatc accaccagcg ccggctcaaa cagccctaac gccagcccca cctgggtatc
     5041 aaattcccgt tcctggttga ccgcggcctg ttcaagcatg gtttccagct cgccgctctg
     5101 ttcgccggag gcgatcatgt acagcatcat cggtggaaac aggtgcaact ccgccagcgc
     5161 ggcgcgcagg ctgcttcctt cgcggacgcg atctgccgcc agcagcagtt gttgctcgac
     5221 ataacgattt gccgacacgg cggcggcggt ctgaatgcct tccagcagcg gaacactgct
     5281 ggcggtgagg atgcttaacg tgcgggaaaa acgcgccgta ttcagtccgc gcgcaacacg
     5341 ccccgtcacg ggcaggcgca gcaaggtttt atcccagcgc aggcgcatgg caggattttt
     5401 gagtagccgt tgccccagca ccagaagccc cagcaaaccc gccagccagt acacgccgct
     5461 ggcctgtaac gcatcgctca tagcgatgag cattcgggtg gaggcgggca gcgcgtgtcc
     5521 gagatgatca aactgttcga taattttcgg cactactgcc gtcagcaaaa tagtgaccac
     5581 gcccgttgcc accaccagca gtaccagcgg gtagagcatg gcctgcaata gacgtgattt
     5641 caggcgctgt cgctgttcgg tgtaatccgc caggcgattg agcaccacgt cgagatgccc
     5701 ggacttttct ccggcagcga ccatcgaaca aaacagggag tcaaagacgc ggggatgttc
     5761 gcgcaggctg tccgacaggg tataaccttc ctgaatccgg ctgcgc
//
LOCUS       DAWDRC010000209         5799 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6276_length_5799_cov_2.395369, whole genome shotgun sequence.
ACCESSION   DAWDRC010000209 DAWDRC010000000
VERSION     DAWDRC010000209.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6276_length_5799_cov_2.395369"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1537 a         1420 c         1382 g         1460 t
ORIGIN      
        1 gtaaagttgg tagattgtga ctggcttaaa aaataattaa ttaataatag gttatgttta
       61 gagagttccc cgcaccagcg gggataaacc gaacggcgcg gcccaccagc aaaaaccgga
      121 tatgagttcc ccgcgtcagc ggggataacc attcaggtat gttcaggccg gacagtttcc
      181 cgcccggcct tcctcactta cgcattcgac cagctctgga tcgaatccgc attccccaat
      241 tccccatgca tcacttcata cgattttttc aggatcacat cagtatgttt ggttaggtct
      301 ttgaaaatgc ctttattcat cgcgtcgtag cgtttggcat tgctctcaat cggtatgaag
      361 atatctttca cgcgaaccat tttatcgaca gcctttgtgt aatccgggta tagccccaga
      421 cctaccgccg tattgatcgc agcccccaga cttgcacaac cgttaatggc gtttcggcgt
      481 gtcggaaggt tgaacacgtc ggcaaaaatc tgcataaaca gatcgctgtt cgaaccgccg
      541 ccagtaatga tcacatgctt tgcaaagtga ttcatttcat gacacatatt gtcgtaatta
      601 ttcttcagcg tcagcgccac gctttccagt atcgaacgat atatccatgc gtaatccata
      661 ctggaatcaa agccgatcat aattccgcgt ttgtacggtt cccacggatt ggtcagccag
      721 tccagcaccg tcatcagccc attacaacca ggtggcacgc aagacgcttt tttgttgagc
      781 aaatcttccg gtgaaagatc ctgtgctttg gcatcctgaa ttaacgactc gcctaacatg
      841 tcgcgcagcc agctcaccgt ccacatgcct ttgcgaatac cgtaaccttc atacagcaat
      901 gtttgcggaa tagaagacat aatcggccag tacgccaccg gatctttcgg cagtgctttg
      961 ccgttcatca tcaacgcgat ataggtgcca agagaaatta ccgccgtttc atcatccagt
     1021 aacccggctc ccagagcttc taccggttta tcactggtgg tacaaacaac cggcagccca
     1081 gccgggaaat gtgtcgccag cgcagcttgt ggtgtgatat gcccaagaat ggtgcccggc
     1141 atttgcacgt caaaaagcat ctggcggggg atattaaact tttccattac cgcggcattt
     1201 tcgctccatg cccagctctt ataatccacc ggccactgac caaaatagtt ggcgatattg
     1261 tctttaaact cgccggttaa gcgatgtgtc agataacccg aaaaagaggt gacataggcc
     1321 acgtcaggat tagtgtgttc gtaagggcgt gtaacgcgtg catcctgcca gctaatcaac
     1381 ggcgcggctg gcgtaccatc ggctttcaat aacgcacggc agcaacggat tgagcccagg
     1441 ccaatgccga caatatcttc tttatttccg gcaaactggc tcatcaaatc gtgactggca
     1501 aaacatagtg atgcccataa atcgtcgtca ggatgttctg cggtatcggc atctggcgtg
     1561 tgcatcggct gcaataagcc tttgccttcg caaaccacat taccttccag atcgtacatc
     1621 accacttttg tgctctgact tccgccatca atccctatga tgtatttttt cgacatgatt
     1681 tttctcctgt aattttcctg gcttacttgc tgctagccat tgcgggggcg ctatcagcag
     1741 tacgaatttt ttggaacagc aaaaaggtaa agatgatcac catgccaagc gccgccatgc
     1801 ccattagcca catgttgcgg taggcttctg cagcgggcag ggtgtcttgc cagtagccga
     1861 taatcgggta aacgaagaca tccggcagaa atccaatcac cgaacaaata cctaccgtgg
     1921 tgcccataat gtaagacggt gttctcgctt cgccagggca ggcccaatac agcccgcgtg
     1981 aggcgtaaca ggtgaatccc agtaataaaa tcaagccaat ccccatcgcg accgattgcg
     2041 ggttagagtt cgtgacgagc agggctgtca atgccagcaa accgatgatg gaaaggattt
     2101 ggaccacgcg ggtaggggat ttcactttac tgtaggtggt gataatgccg ccaagtgggc
     2161 cgcacagcgc gcggaagatt ttgttgatca caatccccat gtagctcgcc gccaccaacg
     2221 acatgccgta catttcggtg agatagttgg tggagtaact cagaatggcg tagatggtga
     2281 agacaccaaa aatcaccatg ctgcaatacc aggtggtgct gatgcgcaaa acggccagga
     2341 tgtcgctaag ctggaatgac tgtttttctt cgttattggc actgcgcaga ttgttgttat
     2401 cgctaacaaa aaaccaacac aaaatgccca gcagaatgta aaccacacta tagataataa
     2461 tgaccgtttt caggctggcg ctgtcatccg gtgcaaagcg agaaaagacc cacatggtaa
     2521 acaccgccag cgacattaca ccgacgccgc gcagcccttc catccagccc ataattttcc
     2581 cttgctcgct atgatcgccc agcaacgatg cggctttaat cgacaccgac cacagcatta
     2641 aaatcgtcgt tatcgcaaag gcgacctgaa tacagagcat tacccacagc ggtggatatg
     2701 ttgccattaa cagacccagt aatccggtaa tgatcatcgc ggaagtaatc attttgcgat
     2761 gtgaaaattt atcggcaata acgccgctag gggcatagaa aataatggcc gcaataccaa
     2821 aggtactcat tattaaacct atctcggtat tgctgaatcc cataaatttt gccatgggaa
     2881 tttgataaat ataacgtaaa taagccaggt cgaaactaac gccgccgcta aaactaatta
     2941 tcgcgagggt tatccaacgg cgatatgagt tgtgttgcat atcgtattcc catttgtaat
     3001 agacaaaaaa aagagaaaag taagccgccc tttcaggtgg attacttttc tcaggtctct
     3061 agtaattgca ataagaaata acatggaaag cagaggtgtt gtgtgagagt aatcacgctt
     3121 ctgctttttt aaattatttg acaatacgaa catcacactg tgattacgat cacattagca
     3181 gttatgtcct tatgctaaaa attatctcca gttaccagag ataatgagaa aggtaacttc
     3241 cctctcgcgg gggaagttac ctttcttttt ttttgcctta cggaaattgg agctaactat
     3301 gtcaatcgaa tctctcaatg cgttctcaat ggattttttc tccctgaaag gtaaaacagc
     3361 aattgttacc ggcgggaata gcggtttagg ccaggcattt tccatggcgt ttgccaaagc
     3421 tggcgcaaat gtctttattc ctagtttcgt caaagataac ggcgaaacaa aggaaatgat
     3481 tgaaaaacag ggtgtcgaag ttgattttat gcaggtggat atcaccgcag aaggcgcgcc
     3541 gcagaagatt atcgctgcct gctgtgagcg cttcggtacc gtagatattc tggttaacaa
     3601 tgccggtatt tgtaagctga ataaggtgct ggactttggt cgtgccgact gggacccgat
     3661 gattgacgtg aacctgaccg ccgcattcga gttaagctac gaagcggcaa aaattatgat
     3721 cccgcagaaa agcggtaaaa tcattaatat ctgttcattg ttctcttact taggtggtca
     3781 atggtcacct gcttattctg ccactaaaca tgctcttgcc ggattcacca aagcctattg
     3841 cgatgaatta ggccaatata atattcaggt gaatggtatt gctcctggtt attatgcaac
     3901 cgatattacg ctggcgaccc gtagtaatcc agaaactaac cagcgtgttc ttgatcatat
     3961 tccggcaaac cgttggggtg atactcagga tttaatgggt gcagccgtat tcctcgcaag
     4021 tccggcatcg aattatgtca acggacattt attagtggtt gatggcggtt atttagtgcg
     4081 ctaattcata acggtggttt ctgttttctc ttattaataa ctattccatt tttattatca
     4141 ggaaggaatt actatgtctt tatctcgcgc agcgattgtc gaccagctaa aggaaattgt
     4201 tggtgcagat cgcgtaatta ccgatgaaac agtattaaag aagaacagta ttgaccgttt
     4261 tcgtaaattt ccggatattc atggcattta tactttgcca attccggcag cggtcgtaaa
     4321 actcggttcc acagaacaag tatcccgtgt gctgaatttt atgaatgcgc acaaaattaa
     4381 cggtgtgcca cgtaccggtg cttccgccac cgaaggtggg ctggaaaccg ttgtggaaaa
     4441 ctcggtggtg ctcgacggct ccgccatgaa tcaaatcatt aatattgata ttgagaatat
     4501 gcaggccacg gcgcaatgtg gcgttccgct ggaggtgctg gaaaacgcgt tgcgtgaaaa
     4561 aggttacacc acggggcatt ctccgcagtc aaagccgctg gcgcagatgg gcggcctggt
     4621 ggcgacccgc agtattgggc agttttccac actctacggc gcaatcgagg atatggtcgt
     4681 tggtctggaa gcggtgctgg cagatggcac cgtcacacgc attaaaaacg tgccacgtcg
     4741 cgcggctggc ccggacattc gtcatatcat catcggcaac gaaggtgcac tatgctatat
     4801 cactgaagtc acagtgaaaa tcttcaaatt tacgccggaa aacaatctct tctatggcta
     4861 tgtgctggaa gacatgaaaa ccggcttcaa catcctgcgt gaaatcatgg tggaagggta
     4921 tcgtccgtcg atcgctcgcc tgtatgacgc tgaagatggt acgcagcact tcacccattt
     4981 tgccgacgga aaatgtgtgc tgatctttat ggctgaaggc aatccgcgca tcgcacaggc
     5041 tacgggcgaa gggattgcgg aaatcgttgc ccgctacccg caatgccagc gcgtggacag
     5101 caagctgatc gaaacctggt ttaacaacct gaactgggga ccggataaag tggctgccga
     5161 acgtgtgcag atcctcaaaa ccggcaacat gggctttacc accgaagtgt ccggctgctg
     5221 gaactgcatt cacgaaatct acgaaagcgt tattaaccgt attcgtaccg agttcccgca
     5281 cgccgacgac atcaccatgc tgggcggtca ctcctctcat agctatcaga acggcaccaa
     5341 catgtacttc gtctacgact acaacgtcgt tgactgtaag ccggaagagg aaatcgacaa
     5401 gtaccacaat ccgctcaaca aaatcatctg cgaagaaacc attcgtctcg gcggttcgat
     5461 ggtgcaccac cacggtatcg gtaaacatcg cgttcactgg agcaaactgg aacacggcag
     5521 cgcgtgggcg ttgctggaag ggctgaaaaa gcagttcgat cctaatggca ttatgaacac
     5581 gggtactatc tatccgattg aaaaataatg tgtcaggcag cgttccgcga tgacggggcg
     5641 ttgccttttc ggcttctcag gcgagaagcc gttcttatta ccggacaatg aaggggtaaa
     5701 gatgaacact tcaccggtgc gaatggatga tttaccgctt aaccgttttc actgtcgaat
     5761 tgctgcgctc actttcggcg cacacctgac cgacggtta
//
LOCUS       DAWDRC010000210         5792 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6279_length_5792_cov_2.686421, whole genome shotgun sequence.
ACCESSION   DAWDRC010000210 DAWDRC010000000
VERSION     DAWDRC010000210.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6279_length_5792_cov_2.686421"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3797..3806
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1584 a         1490 c         1213 g         1495 t
ORIGIN      
        1 cgctggcgac ggcacagttg gtgtggcggt ctcactctgg aacaatttga aaaccagaac
       61 ctcgcttaga gcagtgtcca tattacgagg gtaggatcag tcgtttcttt cgcggtaact
      121 ttatatgatc gctgtgtacc agcatcgaga gaagaaattc aggatgtatt caacactgag
      181 ttcacaagct ctgtagctat taagtcagta ttatttacgt ttacgataca tgttattatc
      241 gcaggatctt tatcttggta gtgatactgc gagtggatac acccccactc agtatcttct
      301 ataactatgg cttttggttc ttcaggcatc ataccttcca taacacgttc ttgatggata
      361 cgtaacttgt agtacttctc tggagatgaa gaaatttcaa cgagtacaga ttgtagatgt
      421 acgaagccta gaaatttaaa acactcggat tttgtagagg attttaatga taaagtccag
      481 ataacaaaca cgttattgga accatcacca taatttttag tgtcaatctt atctaccagc
      541 tcctcgttga ttaacataga ctgatttagc aacttccgct tcacgccctg agcggactgt
      601 tatcgctctt tagacacagt tccactaggg gagaggatca gcgcttccca cgtagggaca
      661 ctatagtaac tatcaccaca tgcagtaggg aagctcaaac caaatttatg tagccagctc
      721 ctattggtgg tcattctggt ggtcttgaca ggaagataac tctggtttag cttacttatt
      781 agccacttac tggcaaaggc gatcccagtc agaggagcca aattcaaaaa agcctgcttt
      841 cgagcaggct ttttactttc taattaccaa cgctcttaaa acatctgtct tgaaccagaa
      901 ctaatttgca caggcattcc cgatcgatgt tgcaacgcag catttgcgcg atttacatca
      961 acttcttgcc cgttgataaa cgcccgcaaa gatggggtta ccggcaatgg cacttttcgg
     1021 tcagactcat attctgcacg attgcgcgac agcggctcat gaacttccag ccactttgaa
     1081 ccatccggtt ccgttgtgta tttcactggc tgatcaataa tttgtacacg agtgcctaca
     1141 ggaacattat caaacagata tttgatatcg tcattacgca gacgaataca gccctgactt
     1201 acacggagcc caataccaaa attggcattg gtaccgtgga tggcatacaa cttgccaata
     1261 taaatcgcgt acagccccat ggggttatcg ggccccgcag gaacaaatgc gggcaaactc
     1321 tcccctcgtt tcgcatattc gcgccgggtg ttcggcgttg gcgtccaggt tggtgcttct
     1381 tgtttacgtt caacggtagt cacccagtta cgcggggttt ctcgcccagc ctggccgata
     1441 ccaataggaa agacttccac tgtattactg tctggcggat agtaataaag acgcatttca
     1501 gcgacgttaa ccacaatccc tttacgaaca gtggcgggca aaatcagttg ctgcggaatg
     1561 gtgagttgtg agccaggctt cggcaaaaaa acatcagcgc ccggattcgc ttccagcata
     1621 ttacttaacc cttgcccgta ttgtgcggca aaagtctcca gcggctgagt attgtgatca
     1681 ggaacagtta cagtaagcga ctgccccact aaacggctac cctctggagg taatggataa
     1741 gttaccgcca ggctattttg gctggcaaaa agcagagcaa atgagcaaag aatatttaca
     1801 cgacgcatca tgtccctttc ctaagtcgcg aatactatcc gataagtata gcttttatca
     1861 gacttttcgt ttttagctgt tcaaatcaga agtcgtattc cccggtagaa caatattact
     1921 ggcagcaagt tcgcccatgt tgttgtatat cgcacaagca gcttcgatga tggacatcgc
     1981 cagagctgca ccactcccct cacctaaacg catatccata ttgagataag gctccagccc
     2041 caaatgcgag agcgctatac gcgcgccttt ttctgccgac aagtgagaag gaatgagata
     2101 cggtttgatt gcaggagaca tctggcaggc tgcgagcgca gcagcataag aaagaaatcc
     2161 atccagcaac acgggtaaac cgcaggaagc agcacctaac atcactccag ctattccgac
     2221 caaatcaaat ccacccactt ttgccaggac atcaacacca tcctgaggat ttggttgatt
     2281 caacgtaatc gcccgacgca caacatcaat tttattagcc agtttatctg tcggcaggtt
     2341 tgcgccaatc ccaaccactt cttcaggatc ccggccagtg attgtgctga ctattgccgc
     2401 tgccggtgtc gtgtttgcca tccccagttc acctacacca aacagcgtga caccgttttt
     2461 tgccagctcc cgcgtataac atatgacgtc caaaagcaac ttttcagcct gacggcgact
     2521 cattgccgga gctgaagcaa tattgccgct acctcgtgcg acacgcatgt tgataagccc
     2581 ggggataggc tcagcagtat caataccaac atcaattacg tggacgttag cgcccgcttg
     2641 tgctgccagc acacacacgc cggttgttcc acgggtcata ttttcagcct gtatggctgt
     2701 cacttctttt ggagaaatag cgcccccttc ctcccagacg ccgtgatcgg cacacataac
     2761 cagtactgct tttttgccca catgcggtat gccattcaac cccggcattc ctgccagttg
     2821 tatggcaagc gcctccagct ttcccaggct accaacaggt ttgagtaacc cgtcaacatg
     2881 ccgttgtgca cgcgacatag cggcagaatt gatggctggg atcgtattca gtaaatcggc
     2941 aagtatttgc atgtcacgtc tctttatagc agtgccagca ggaaaaccag ttcaccaaac
     3001 tctatggctg ctcccagcgt atcgcccgtt tgcccgccca gcgtacgttt gagaagctga
     3061 ccgaggatga aaatcgccac catcgtcacc accatagcag ccacaccatg catacccggc
     3121 aataatacgg cagcaaaaat agcggctaag ccgagtgtga cgcaggtttg tcgcccatca
     3181 attttgccaa tgaacacatt gccaagccct tcttcccgcg cataacgatg acgatacatc
     3241 aataatgcgg cagtcccgcg gctgaccgca catgctgccg ccagcgatgc aaggatcggt
     3301 tcgccacgca gagccaactc gcttaacacc agaatctttg ccagtaccac aaaaatcaat
     3361 gccagaccgc cgtgggtgcc taaacggcta tcacgcatga tttccaacat gcgatcgcgg
     3421 ctacgtgcag aaaatacgcc atcacaggta tcagcgaggc cgtccagatg gaatcccccg
     3481 gtcatcagcg ccagcacaag tacgctaaac agtgccgcca gtggtacgcc acaccatgcc
     3541 tgcagcacca tgaagaccag cccgctaatc gcgccaagca ataatccaat caaaggaaaa
     3601 gtaataatac cgcgagaata atgctcgaaa tccagtccct gggaccagcg acgcggtaca
     3661 ggcagacgcg taataaatga gagcatcgcc caaaataatt tactcatttg atttttactc
     3721 caatacccga aaccaccagc catacttcat ttgccgcagc ggccaactgc tgatttaccc
     3781 gcccggcaat atcacgnnnn nnnnnnttgc ggggcaacgt tggcaggcag caatcaacga
     3841 ctgaatctca gcattaatcg cctgttccat cgcctgataa tcccattcat cagggtcttt
     3901 atcgccgcca taatcaaaca acagattagt caccattgtg gtaacgcatt ccagcaacac
     3961 agcctcattc gggttaatgt ctgcatgaat taattcatca agatgttgcc agcgctccac
     4021 tgtgcgccag tgctccgggc ggctttgccg atgatgttct atccgtgcag ccatctcatc
     4081 atcaaggatt tgcgaggtag cgatatacag aacctgtgaa gagtccccaa taagcacctc
     4141 tgcgtggcga ctcttcccgc tccgtgcgcc tcccgtcacc aaaatcatca taccgactcc
     4201 tgattcaaat gtatgatggt attcattgaa cagagcaatg gatcccccac cgttgctgac
     4261 cgcaaaacat ttacgcgatt aatactgcgc gtaatataat taaaaatcca gcattctcaa
     4321 tccattacga aataagatgg cattacggaa tagccagact ttctcgtgtt aacgtccgac
     4381 aaaatgcgtc attgacagcg gtttctgtgg attgacacat ttcattcaga taatgaatta
     4441 atgctactgc cggaccaacc agcaaacggg cattgtgttc tgaaatccag gctattgatt
     4501 caacgtcagc gacagatctg cgctgtaaaa gaagttgtaa aacaacgcaa taaaattatc
     4561 cggaatggtg gcgactatgc actagaacac cacaatttcg ctctctcggt aaggcactgt
     4621 cacagcattc aacagaatgt gacttgccag atttattagc gccaccagat gtttaaccgg
     4681 gtaaccacga cccagtcggg gatatattcc atcttctcgc tgacatcatc aagaataaaa
     4741 aggtttatca cactcagagc aacgttcttc agcaggcggg ataattcttc aacatttaaa
     4801 agatgtgtta ttaaaggcat acaaactgga cgtattgtat tttcttttgg tggttgacct
     4861 aaggtagcag tttatcctga tgcgctgaga tttctgtaat atctacgtca acattctcct
     4921 cgaatagaaa atgcagcctc ggcaagtgat tcacattttt taacaaagcg ttgttagcgt
     4981 gccattttca gaagctctcc aagaaagcga attcttttct caaattctgc attctcagac
     5041 acagctttct actccatggc aatggcccaa atacgaagtt gctcaggatc attagtggtg
     5101 ctgagaagtt aaataaccat gcggagtacc actcgtcatc acgtatttat atatcatgaa
     5161 gctattgttg tgttttgtac atcagtagat gtattctgat atactccttt ttctagacat
     5221 aacctttcac ctgcttgcaa agcttctgtg ttctgacatt gccaaattgt tgcaattctg
     5281 tatccagcct tctttcagtc atagcttcgg gccgcgataa gactcactga tctgaccctg
     5341 attcctcttg cagactttat agaccaatta aaatgcagtt tctgcaggtc aacgtctgac
     5401 catcattgtc atcactctgg ccattagagt aaccttctgc attcatcctt ttgtaaaaag
     5461 tttatgtcag tatcagcaat taaccggacc tgatactgat atgagtctta ccgcatatac
     5521 ggtcaatttc agcaattaat tacattatcc acgccaaagt gtttgtcatc gcaatgatgg
     5581 taccttcttt cagacaccat tttttcaact ccgttttcca cggaccgcac tcttatgtca
     5641 agagtgcggt ccgtggatac aaccagagac cgactgacac gagtcagagg aaacgacgga
     5701 tatgttcagt cgtaaaatat ctatcaaaaa acatgattaa ggtcaaaaat gtttgatatt
     5761 tacaatttat gaagatgaca ataatcacag at
//
LOCUS       DAWDRC010000211         5755 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6307_length_5755_cov_2.396316, whole genome shotgun sequence.
ACCESSION   DAWDRC010000211 DAWDRC010000000
VERSION     DAWDRC010000211.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6307_length_5755_cov_2.396316"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1419 a         1460 c         1518 g         1358 t
ORIGIN      
        1 atattttcaa agatgaaggg cgcgaagcgt ttctgaaatt aatggaaacc accgatatct
       61 tcatcgaagc cagtaaaggt ccggcctttg cccgtcgtgg cattaccgat gaagtcctgt
      121 ggcagcacaa cccaaaactg gttatcgctc acctttccgg ttttggtcag tatggcaccg
      181 aggagtacac caatcttccg gcctataaca ctatcgccca ggccttcagt ggttacctga
      241 ttcagaacgg tgatgttgac cagccaatgc ctgccttccc gtataccgcc gattactttt
      301 ctggcctgac cgccaccacg gcggcgctgg cagcactgca taaagtgcgt gaaaccggta
      361 aaggcgaaag catcgacatc gccatgtatg aagtgatgct gcgtatgggc cagtacttca
      421 tgatggatta cttcaacggc ggcgaaatgt gcccgcgcat gaccaaaggt aaagatccct
      481 actacgccgg ttgcggtctg tataaatgcg ccgacggcta catcgtgatg gaactggtgg
      541 gcattactca aattgcagag tgctttaaag atattggcct cgcacatctg ctcggtacgc
      601 cggaaatccc ggaaggcact cagcttatcc accgtatcga atgcccttac ggcccactgg
      661 tagaagagaa actcgatgcc tggctggcgg cacataccat cgcagaagtt aaagaacgct
      721 ttgccgaact gaatatcgcc tgtgccaaag tactgaccgt gccggaactg gaaagcaatc
      781 cacagtatgt ggctcgcgaa tcaatcactc agtggcaaac gatggatggt cgcacctgca
      841 aagggccgaa tatcatgccg aaattcaaaa ataaccccgg acaaatctgg cgcggaatgc
      901 cctcacatgg catggacacg gctgccattt tgaaaaatat cggctacagc gaaaacgaca
      961 ttcaggagtt ggtcagcaaa ggtctggcca aagttgagga ctaaacgcgt ggcgtcagtc
     1021 aacttgcctg actagcgccc gcccaataac aaacgaacaa aatggataga ggtgcaatgg
     1081 atatcattgg cggacaacat ctacgtcaaa tgtgggacga tctggcggac gtttacggtc
     1141 ataaaacggc gctgatttgt gaatccagcg gcggagtcgt taaccggtat agttatcttg
     1201 agttaaatca ggagattaac cgcacggcaa acctgtttta tacgctgggg attcgcaaag
     1261 gcgacaaggt tgctctacat ctcgacaact gcccggaatt tatcttttgc tggttcgggc
     1321 tggcaaaaat tggcgcgatt atggtgccaa ttaacgcccg cctgttgcgc gaagaaagca
     1381 cgtggatcct gcaaaatagc caggcgtgcc tgctggtgac cagtgcgcaa ttttatccta
     1441 tgtatcaaca gattcagcag gaagatgcct ctcagttgcg gcacatttgc ctgatagata
     1501 tggcacttcc cgctgatgat ggcgtgagtt cgtttactca actgaaaaat caacaacctg
     1561 ccaccttgtg ctatgcaccg ccgctatcga ctgacgatac ggcggaaatt ctcttcacct
     1621 ccggcactac ctcccgaccg aaaggggtgg tgattaccca ttacaacctg cgcttcgctg
     1681 gatattactc cgcctggcag tgtgcactgc gtgacgatga cgtctacctg acggtaatgc
     1741 ctgcgtttca tatcgattgc cagtgtactg cggcgatggc ggcgttttct gccggggcca
     1801 cctttgtgct ggtcgagaaa tacagcgccc gcgccttctg gggacaggtg cagaagtacc
     1861 gcgccaccat taccgaatgt attccgatga tgattcgtac gttgatggtg cagccgcctt
     1921 cagcgaacga tcggcaacac cgcctgcggg aagtgatgtt ttatctcaac ttgtcggaac
     1981 aggaaaaaga cgcattttgt gaacgcttcg gcgttcgctt gctgacgtct tatgggatga
     2041 cggaaaccat tgtgggcatt atcggcgatc gccctggcga taaacggcgc tggccgtcga
     2101 ttggtcgggc gggcttttgc tacgaagcgg agatccgcga cgatcacaat cgtcccctcc
     2161 cggctggtga gctcggtgaa atctgtatta aaggcgtacc agggaaaacc atcttcaaag
     2221 agtattttct caacccgaaa gccactgcga aagtgctgga agccgatggc tggctacata
     2281 ccggcgatac cggataccgc gacgaagagg gcttttttta tttcgtcgat cgccgctgca
     2341 atatgatcaa acgcggtggc gagaatgtct cctgcgtgga gctcgaaaat atcatcgcca
     2401 cccatccaaa aattcaggat atcgtggttg tgggtattaa agattcgatt cgcgatgaag
     2461 ccatcaaagc atttgtggtg ctgaatgaag gtgaaacatt gagcgaagag gaattcttct
     2521 gcttctgtga acagaatatg gcgaaattta aagtgccctc ttatctggag atcagaaaag
     2581 atctgccacg taattgctcg gggaaaataa ttagaaagaa tctgaaataa atgaaacggc
     2641 agggaactac cctgtcagct aataatcacg ctctaaaaca atatgcattt tttgcaggga
     2701 tgttgtcgtc cctgaaaaag caaaaatgga gaaaaggaat gagtgaatca ttacatctga
     2761 cccgcaatgg atcaattctg gaaattaccc ttgatcgtcc aaaagcgaat gctattgatg
     2821 caaaaaccag ctttgaaatg ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac
     2881 gtgtcgccat tattaccggt gccggagaga aattcttttc cgcgggctgg gatttaaaag
     2941 cggcagcaga aggcgaagca ccggatgctg actttggccc gggaggtttt gcgggattaa
     3001 ccgaaatttt caatctcgac aaaccggtca tcgccgctgt gaacggctat gcctttggcg
     3061 gcggctttga actggcgctg gcggcagatt ttattgtttg tgccgataac gccagcttcg
     3121 ccctaccgga agccaaactg ggcatcgttc ctgacagcgg cggcgtgctg cgtctgccga
     3181 agatcctgcc gcctgccatc gtcaatgaaa tggtgatgac cggcagacga atgggcgcag
     3241 aagaggcgct gcgttggggg gtagtcaacc gcgtggttag ccaggcggaa ctgatggata
     3301 acgcccgcga actggcgcag cagctggtta acagcgctcc gctggcgatt gcggcgctga
     3361 aagagatcta ccgcaccacc agtgaaatgc cggtagaaga agcgtatcgc tatattcgca
     3421 gcggcgtgtt gaaacactat ccatcggttc tgcattcgga ggatgccatt gaagggccgc
     3481 tggcgtttgc cgagaagcgc gatccagtgt ggaaaggacg ttaacaaccg tgagctatta
     3541 cgcctttgag gggttaattc cggttgttca cccgacggcg tttgtccatc ccagtgcagt
     3601 attgattggc gatgtgattg tgggagccgg tgtctacatc ggcccactcg cctcactgcg
     3661 tggtgactac gggcggttga tcgtgcaagc gggagccaat attcaggatg gctgcattat
     3721 gcatggctac tgcgacactg acaccatcgt tggggaaaac ggtcatatcg ggcacggagc
     3781 gatcctgcat ggttgtgtga ttggtcgcga tgcactggtc gggatgaaca gcgtgattat
     3841 ggatggcgcg gtcattggcg aagagagcat tgttgccgct atgagctttg tcaaagcggg
     3901 ctttcgcggc gagaaacgcc agttgttgat gggtacgcca gcccgcgctg tacgcagcgt
     3961 gagtgacgac gagttacact ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg
     4021 gcgctgtcat gcagcgttac atgaaacgca gccactgagg caaatggagg aaaatcgccc
     4081 ccgtttgcag gggacgacgg atgtgacgcc gaaacggtaa catcgtcgga tgcggcataa
     4141 acgccttatc cgacctggct gtaaagcaac cgtaggccgg ataagatgcg ccagcatcgc
     4201 atccagcgac caggtcgcac ggtatcaacg aaatgacatt aacgacgcat actctttgac
     4261 aacatcatct gccacttctg ctcgcggtta agttccgtga gcggaacggc aggaatacgc
     4321 ggcgtctttc ttacgccgac taatttatcc cggctgttat tgcgcaggcg cgcgtaaatt
     4381 tgctcatcga tatcaaccac ttttaccagt cgctggcact ggcatccccg cccttccagc
     4441 ttattaggga tcatcttaac ttcgcagctt atttctgtga cttccgacag aatataagtc
     4501 agggtattaa ccgccttgct gtgttcaata tcgaaatgaa tagagatctc tcttgctatc
     4561 acccaccggt tttcagccat catccattcg gcgattaaca aatagagtgg tttttcaaca
     4621 catccttcac acatacgaac tccagtttca caaattaaaa gactggctat gactcgtttt
     4681 gcagcaatta aacaaggcca tattaaaatt gtggatatat cgatagtatg caacttcacc
     4741 gtcgataagt gtgagctaaa tcctgcttat gtcatcaaaa atatcgattc cccaaaagat
     4801 ttacttaatg ggcaaaaaaa aacggtcctc atcagagaac cgtattagtt attcgatcaa
     4861 attagcggat gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc
     4921 tgcaccgaaa ttactttttc ggtcgcatcg gcattcagcg ccatcgcggt ggcaaagccg
     4981 ccgttcaggg tggtgtcgta atgcacttta tattgcagcg cactgcgacg gatcacgcgg
     5041 gagtcttcaa tcgcacgacg acctgaggtg gtgttgatga tgtaggtata ttcgccattc
     5101 ttaatacggt cctgaatgtg cggacggcct tcatgcacct tgttcaccag acgcggattg
     5161 atacccgctt cgcccagcac aatcgccgtg ccgtgagtcg catccagctc gaagccctgt
     5221 ttcagcagtt ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc
     5281 agcgcacgac cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct
     5341 tcagcgaagg tgcggcccac gcccattact tccccggtag agcgcattta ttgaacggca
     5401 gcaccacttc tttcaccgag tagtacggcg ggataacttc tttggttacg ccctgctcag
     5461 ccagcgattt gccagccatc acacgcgccg ccacttttgc cagcggtacg ccggtggctt
     5521 tggagacgaa cggaacggta cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt
     5581 tgtttttcac cgcaaactgc acgttcatca ggccgcgcac ctgcagttcg aaggccagtt
     5641 tctgcacctg ctggcgcatc acatcctgaa tttcctgact taaggtgtaa gctggcagtg
     5701 aacatgcgga gtcaccggag tgtacgcctg caagctcgat atgctccata acggc
//
LOCUS       DAWDRC010000212         5752 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6311_length_5752_cov_1.900298, whole genome shotgun sequence.
ACCESSION   DAWDRC010000212 DAWDRC010000000
VERSION     DAWDRC010000212.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6311_length_5752_cov_1.900298"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3833..3842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1398 a         1546 c         1361 g         1437 t
ORIGIN      
        1 cgtatatttc cagttttaat gtttccgtgt cggcagtccg gtacgcagca attgttgtaa
       61 atgctgctgt gcctgcgggg agcgtttcgc cccttcagct tcaaacaaat gatgccactg
      121 ttgcagtgcg ttactgctgt tcggtaagcc agccataacc ctgctcctcc agttgtttga
      181 ccaacgtgaa atcgccggat ttcacaattc gtccctgata cagcacatgg acgtaatcag
      241 gcttgatgta gtcgagaatg cgttggtagt gcgtaacaat gatgaatgaa cgcttgccgt
      301 cacgcagcga gttcacgcca tcggcaacca cttttaatgc gtcaatatcc aacccggagt
      361 ctgactcatc aagaatgcat aactccggtt ccagcaccgc catttgcaaa atatcgttgc
      421 gctttttctc gccgccggaa aaaccaacgt ttaccgaacg ggttaataaa tcttccggca
      481 tcttcaggag agcgattttc tcttccatca aatcctgaaa atcaaagcgg tcgagcgttt
      541 cctgcccgcg atagctgcgc accgcattaa gtgccgtttg caggaaaaac tggttactga
      601 cgccgggaat ttccaccgga tactggaagg ccataaagat gccttcacct gcacgatctt
      661 ccggcgacag cgcaagcaaa tctttgcctt taaactcaac cgtgccgctc gtcacttcat
      721 aatcttctcg cccggcaagc gttgccgata aggtactttt gcccgaacca ttgggcccca
      781 taatggcgtg aacttcgccg ggacgaacgt cgaggcttaa tccacgcagg atagctttat
      841 cttccacgct gacgtgtaaa tctttaatac ttaacatgtt tattccttat ccgacgctgt
      901 gttcaagact gatggcgagg agtttttgtg cttcaacggc aaattccagc ggcagttccg
      961 agaacacgtc tttgcaaaaa ccgttaacaa tcatcgagat ggcatcttct tcgctgatcc
     1021 cgcgttgcag gcagtaaaac agttgatctt caccaatacg tgatgtcgtt gcctcgtgct
     1081 ccagctgagc gctgttattg cgacattcga catacgggaa ggtatgcgcc ccacaattag
     1141 cgccaatcag cattgaatcg cactgagtga aattgcgcgc attggttgcc gtcggcatga
     1201 ttttcactaa gccgcgataa ctgttctgac tatgtccggc agagatccct ttcgagataa
     1261 tggtcgattt ggtgttttta ccgatgtgga tcatcttggt gccggtatcc gcttgctgat
     1321 gaccgctggt cagcgccact gagtaaaact caccaatgga gttatcgccg cgcaaaatgc
     1381 agctgggata tttccacgta atcgctgagc cggtttctga ttgcgtccat gacattttgc
     1441 tgttttcgcc ttcgcacaaa gcacgcttgg tgacgaagtt gagaataccg ccggtgttgt
     1501 tatcgccagg gaaccagttt tgtaccgtgg aatatttcac ctcggcgttt ttatggatga
     1561 tgacttccac caccgccgcg tgtaactgat agctgtcacg caccggagcg gaacagcctt
     1621 caatgtagct aacgtagctg tcttcgtcgg ccaccagaat ggtgcgctca aactgcccgg
     1681 ttttttccgc gttaatgcga aaataggtgg aaagttccat cgggcaacgc acgcctttag
     1741 gcacataaat aaacgtaccg tcagaggcta ccgccgcatt aagtgcggca aagaagttgt
     1801 cattccccgg caccacggtg ccgagatatt tacgcaccag ttccgggtga tcgtggatcg
     1861 cctcgccaaa ggaacaaaaa ataattccct gctccgccag ttttttgcga tgagtagtgg
     1921 caaccgaaac agagtcgaaa atggcatcca ccgccacctc tttgccttcc cgcacgggaa
     1981 cgcccaactg ctcaaacgcc gcctccacct ctttacttaa aaaggcgttc gcgccagttt
     2041 gctgcaccgc gccgggttca gacgcgcagt tgtcgtcaca attaccgcac gatggtgccg
     2101 agtagtagct gtaatcctga taattcagct tgtcgtagtg cgctttcaac cagtgcggtt
     2161 cttccatctc cagccatgcg tgataggcgt tgagacgaaa ctccagcatc cactccggct
     2221 cattacgctt cgccgaaatt gcgcgcacca cctcttcgtt tatccccttt gccagctcat
     2281 cggtggctaa ctgggtgaag aatccttctt tataattcag cgggccgccg gtccaggttt
     2341 tgacatcgtc agttgcttca gtattacgag acatagtacc gcctataccc caaagctttc
     2401 gccacagcca cattcattct gggctttagg gttgtgaaat ttgaatatct gattaagtcc
     2461 ttcacgaacg aaatcgactt ccgtgccatc aataaaaggc atcgcttgca gcgggacaaa
     2521 cagcttcgcg ccgtcgtgtt caaacagcag atcgtctttg tccggctcgc taacactgtc
     2581 gagcacatag ccaaagcccg cgcagcccgt ttgcttaacg cctaagcgca cgccgatcat
     2641 accaggctgc tttgccacca gttcgcggat gtgtatcgcc gctgcgggtg tcagcgttaa
     2701 gccttgccag gcgaaatcct gtggattaaa ggttcctgaa tgcatgtcca tcgatttacc
     2761 tcacttcatc gctttcagcg tataacagca tgttagtgat aatgattatc agttcaaccc
     2821 agcaaacgca ggggctttag cgtaaaacat gctttttacc tgcttttaat aagcatagac
     2881 cctgatgtgt gggttaacag gcacgctaag aattaggtat ctcattgtta gataatgatt
     2941 attatctaag gagcattaag gctgtgatga aaaagaaaaa gttgtattga aaatgactat
     3001 ttaagagata ggtaaaaaag tgcagcgttc agaaataaga aaacccttaa gtctgtgcga
     3061 cacaggctta agggtttcta ccccatccgg cgcttatctc cggcactctc agtggcttag
     3121 ctcttgaagg ggcgataagg ataatctcat aaagctaacc cactgtttta acacaaactg
     3181 cgatttgtat tatttttgaa cgttatcagg cgggtagata ggctgtatta aaaaggccct
     3241 cgaacctgga cgaagattaa tccaggccga tttggcgaac tcgcgacatt aaatcaggat
     3301 caatgcacgc ccagacgcca ggcgaagtag atttcttctt ttaattcagc agaagagaga
     3361 gtaagcaggt cagcaaattc aagttctaat tgtttcagac gcttgagata ttgggcaggt
     3421 gaaagattgc tttggtcacg gcgtaaaaat tcaatggcca gttgggtggg atcaagttga
     3481 gtagacatag catcctcgct tttagacaag acctgcacag tataccaccg tttactgtgc
     3541 agataataac caaaagcaat atgcgtcaca cttttctggt gacaacgtca caaaatagcg
     3601 gtcgtcaatc gtgacgaaca gcacaaacgc cctttctcat cgaagatttc aatctgccag
     3661 acctggtgac gcgaaccgag atgcaacggt ttgcatacgc cgcgcacccg cccttctcgt
     3721 gccgagcgga cgtggttagc attgatttcc agaccaacca ccttttgctc accttcggta
     3781 cataaataac cggcaacgga accgatactt tcggccagta ccacggatgc ccnnnnnnnn
     3841 nncccctcca tgcagcaacc cgaaaggctg ctttgtccgc gagtctactg gcattgtcgc
     3901 ttcaagggtg tcatcaccaa tatgttcaaa gcgaatatcc agcaatccca ccatgtttcc
     3961 ttcccccata gcattcagtg cttccagggt gatttttcgt ttccatatca tttaataatc
     4021 tccagtaaag cctgcacagg atggcgtacc cctgtgcctt caacccgttt tacctgacta
     4081 cggcaggaat atccggtcgc cagacagcgg ttacgcggta gtcgctgcat cgcctgatgc
     4141 caggataact catagatccc gagcgaattt ttatggtttt tcgcttcatg tccgtaagtc
     4201 cctgccatgc cgcagcaccc cacgctgaca ttttccagtt tcgcgccaaa acgggcaaat
     4261 attgccgccc attgtgctgg cgcgcccggc aaggcggtaa cttcggtaca gtgaccaaag
     4321 aaataccatg attcaccgct gactgtagcc actggctgtg actcaagtgc gcttgccagc
     4381 cattcattcg ccagtaagac gttaaactcg ccacgctcct cgcccagagc cagtttatat
     4441 tcatcgcgat aacaaagtac cagcgccggg tcgacgccta ccattggcat acccagcttc
     4501 gccatacggt tgaggaaatc cgccgtcttt ttcgccgtct tcgcaaaacg attaagaaaa
     4561 cctttgatat gctgggcttt gccatttggc gaaaatggca gtaacacggg ctggaaacct
     4621 aatttttcga ccagacggac aaaatccgcc accacttgag catcgtaata gctggtaaag
     4681 gggtcctgca ccaccagcac cgtgcgcgct ttctgctctg cactgagagc ttcaagctgt
     4741 tccagcgtca tgtttgccga gcgatgcccc accatttgtt gttgtagcga ggggactgac
     4801 agcagcggca aatcaaccat gccgatatgt ttttccgaga gtttgcgcac cagcggctgg
     4861 ttaatgaaga agttaaaggt tttcggcgcg cgtgccatca gcggtgcgta actctcaacc
     4921 gtagcgacga ggtggtcgcg cagcgggcgt aaataacggg tgtgatagag ttgcaggaaa
     4981 cgagagcgaa actccggcac atcaattttg atggggcact gagtcgaaca agctttacag
     5041 gccagacagc ccgacatcgc ctctttgact tcgtgtgaga agtcatattc gcctttattc
     5101 gcatgccaac tattgcgcgt gcgggcaatt aacgtccgca aactgactcc ggattcaggc
     5161 agttcttgtt ccagtttgag tgggtcaacg ccgcgatccg ccaacaaacg cagccattca
     5221 cgtaccagcg ttgcgcgccc tttcggtgaa tgaatccggt tctgggtgat cttcatcgac
     5281 gggcacatcg gactacgagc atcaaagttg aagcataaac cgttgccgtt acactccatt
     5341 gcaccacgcc actgctggcg taccgcaatg gggatctgtc gatcgaatgt accgcgcttc
     5401 accgcgtcca ctttcatcat cggcgcatcg agaccttctg gcgggcaaat cttcccgggg
     5461 ttgagtcggt tatgcgggtc aaatgccgct ttcactttac gcagttctgc aaaaagttcc
     5521 tcaccgaaaa acgccgggct gtattcggcg cgaaaacctt tgccgtgttc gccccacaac
     5581 aaaccaccgt atttcgcggt aagcgccact acatcatcag agatttgctt catcaaaatc
     5641 tcttgttgcg gatcgcacat atccagtgcc ggacggacgt gcaagacacc tgcgtcgacg
     5701 tgaccgaaca taccgtagct taagccgtgg ctgtcgagca gcgcgcgaaa tt
//
LOCUS       DAWDRC010000213         5739 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6329_length_5739_cov_2.119282, whole genome shotgun sequence.
ACCESSION   DAWDRC010000213 DAWDRC010000000
VERSION     DAWDRC010000213.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6329_length_5739_cov_2.119282"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3698..3707
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5348..5357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1474 a         1399 c         1334 g         1512 t
ORIGIN      
        1 attgtgagta caaaatttct acacttcctc aaaataattg cacaatatgc taaaaaaata
       61 tgaatgacat gctgttttat tgttcatata aatcttaaaa gcagaaattt atctgctccc
      121 ccctttaaaa attcggtgaa tacccttact tattggtata ctgaagttgc ttcagataaa
      181 aacgcattga acctcgaata acgttgtcta gtaacacgaa ttagggggcc atgatggaaa
      241 aaaataatga agtcattcag actcatccgc tcgtagggtg ggacatcagc accgttgata
      301 gctatgatgc gctgatgttg cgtttgcact accagacccc aaataagtcc gagcaggaag
      361 ggactgaagt tggtcagacg ctctggttaa ccactgatgt tgccagacaa tttatttcga
      421 tattagaagc aggaatcgcc aaaattgaat ccggtgattt ccaggtaaac gagtatcggc
      481 gtcattaaca tgacatgacc atccacccaa aaggcacctc accggtgcct ttaccatttc
      541 tgggttgtat atctgcattc agttaattac tctgcttaaa ccatgagagc agagagaaaa
      601 aaatgaaata cgatctcatc attattggca gcggttccgt aggcgctgcc gccgggtatt
      661 atgcaacccg cgccggttta aacgtgctaa tgaccgacgc ccatatgcca ccgcatcaac
      721 acggcagcca ccacggcgat acgcgattaa ttcgccatgc ttatggtgaa ggcgaaaagt
      781 atgtcccgct ggtcctccgc gcgcaaatgc tgtgggatga actttcccgc cacaacgaag
      841 atgatcccat ttttgtacgc tctggtgtca ttaacctcgg tccggctgac tccgcatttc
      901 tcgccaacgt cgcccacagc gccgaacagt ggcaactcaa cgttgaaaag ctcgacgcgc
      961 aagggattat ggcccgctgg ccagaaatac gcgtcccgga caactacatc ggtttatttg
     1021 aaactgattc cggttttttg cgcagcgaac tggcgattaa aacctggatc caactggcga
     1081 aggaagcggg ctgtgcgcag ctgttcaact gcccggtcac cgcaattcgt catgacgatg
     1141 atggcgtaac tattgaaacg gctgacggtg agtatcaggc gaaaaaagcg attgtctgcg
     1201 cgggaacatg ggtaaaagac ctactcccgg agctgcctgt ccagcccgta cgcaaagtat
     1261 ttgcctggta tcaggccgat ggtcgctata gcgtgaagaa taaattcccg gcgtttaccg
     1321 gtgaactgcc caatggcgat caatattatg gttttccggc agaaaacgac gcgttgaaga
     1381 ttggcaaaca taacggaggc caggttatcc attcagcgga tgaacgtgtt ccgtttgcgg
     1441 aagtggtcag cgatggttcg gaagccttcc cgttcttgcg caatgtgttg ccgggtatcg
     1501 gttgctgcct gtacggcgct gcctgcacct atgataattc gcctgacgaa gattttatta
     1561 tcgataccct acccggccac gataatacac tgctcattac cggcctgagt gggcacggtt
     1621 ttaaatttgc gtcagtttta ggggaaatag ctgccgattt tgcgcaagac aaaaaaagcg
     1681 attttgattt gacgccattc aggctttccc gcttccaata atcatcatta cggcctccag
     1741 aatgaggccg tttttacagg agtatttaat gcgtcgactg ttgcactatc tcatcaataa
     1801 tattcgcgag catctgatgc tttatctttt tctctgggga ttgttggcaa ttatggatct
     1861 gatctatgtg ttttattttt agttttccgc actgacataa actgacaaat atttaatctc
     1921 cctttcattt agcgtgaaaa attgaatgag attcttcctg cctctacagc actcactaga
     1981 ccttccagat gcttaaccca ctaaacttat gaagattctt tcattattaa ttcaaaatta
     2041 ttgaaataaa aataaatatc attcaatttg ttatttttgt gttgcaattc attctccgac
     2101 acgccatttt ctttctctcg caaattcgtt ttggatgtac ttatgtcatt cacaaatacc
     2161 cctgaacgct atggtgttat atctgcggcc ttacattgga taagcgcaat tattgtctac
     2221 ggcatgtttg ctttaggtct gtggatggtc acgctcagtt attacgatgg ctggtatcac
     2281 aaagcacccg aactgcataa aagcatcggt attttgttaa tgatggggct ggttattcgc
     2341 gttctgtggc gtgtcatatc tccaccgccg ggtccgctgc cgagttattc gcccatgact
     2401 cgtcttgcag caaaagctgg acatctcgca ttgtacctat tgctgttcgc catcggcatc
     2461 agcggctatc tgatctcaac tgccgatggt aaaccgatca gcgtttttgg ctggtttgac
     2521 gtccccgcga ccctctccga cgctggcgca caggctgact ttgccggtgc cctgcatttt
     2581 tggctcgcgt ggagcgttgt agtactgtcc gttatgcacg gatttatggc cctgaagcat
     2641 catttcatcg ataaagacga cactctgaag cgcatgctgg gaaagtcgtc atctgactat
     2701 ggagtataaa aaatgaaaaa aagcctgctt ggtttaacct tcgcttcctt gatgttctct
     2761 gccggttcag cggttgccgc cgattacaaa attgacaaag aaggtcagca cgcctttgtt
     2821 aacttccgca tccagcatct tggctatagc tggttatatg gcaccttcaa agatttcgac
     2881 ggtactttta cctttgatga aaaaaatcca gctgccgata aagtgaatgt gacaattaac
     2941 accaccagcg tcgatactaa tcacgccgaa cgcgataaac atcttcgcag tgcggatttc
     3001 ctcaataccg caaaatatcc acaggcaaca ttcacctcca ccagcgtgaa gaaagacggt
     3061 gacgaactgg atattaccgg caatctgacg ctgaatggtg taaccaagcc cgtcacactg
     3121 gaagcgaaat taattggtca gggtgacgac ccgtggggcg gtaaacgtgc aggctttgag
     3181 gccgaaggca aaatcaagct caaagacttc aatatcaaga cagatttagg tccagcgtct
     3241 caggaagtgg atctgattat ttcagtggaa ggcgtacagc agaagtaaga gtgaaatgtt
     3301 gccggatggc acgcatccgg caatgatatt tattctgtcg gttcaggaat gcccagtgtt
     3361 gtattcagac gtccacgaga cttattaaag atcttattgc cattttcacg tcctgcccga
     3421 cggcgtcgct gttcctctgg cggtaacgcg ctttcttcgc agcaaatctc actacaacaa
     3481 cctttgtatt tttccgcaca tactggacac tgaataaaca acagatggca gccatcattt
     3541 ttacagttgg tatggctgtc gcacggcgca ccgcactggt ggcagtgcgc gataatctca
     3601 tcagatatac gttcgcccat ccgctcgtca aaaacaaaat ttttgccaat aaaacgcacc
     3661 ggcaagccct gctcgcgcgc cttacgggcg tattcaannn nnnnnnntat tcaataattc
     3721 cgccctcgat atgccagact ttgttgaatc cgttatgttt catccaggca ctggcctttt
     3781 cacaacgaat gccgccggtg cagtacatga cgattttttt atctttatgt gcctgcatca
     3841 tctcaactgc ttttggcagc tgctcacgga aggtatctgc cggtatttcc agcgcgtttt
     3901 caaagtgccc cacttcatac tcatagtggt tacgcatgtc gataaacaat gcatcgggat
     3961 cgtcaagcat ggcgttcact tccgccgctt gcagatactc accaacattg ctggcatcaa
     4021 agtgaggatc gtcaataccg tcggcaacaa tgcgatcgcg taccttcata cgcagtaccc
     4081 agaaggattt cccgtcatca tccaacgcga tattcaggcg taaaccctct aaagccgggt
     4141 cgaaggcata gagctgcgcg cgaaatgttt caacattgct cgcaggtacg ctgatttgcg
     4201 cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac attcagcgcg gtaaacagtt
     4261 gatataaagc gtcacgggtc accttaggat cggcaatgtg gaaatactta taaaacgaaa
     4321 tggtggttcg cggttcgctc tcagccaaca ttttggcttt taacgcgtcg ttggaaatgc
     4381 ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat gggaaagtaa aaatccgcgg
     4441 catgatatag caattatcga taattaacat ccacacattt tacgctacat tttagcatta
     4501 aaaattattt gttatttaca agcgcggcaa tttcgcccag tcttcagtca caaattttgg
     4561 ttgcggacga aaaaatgcga caatacatac aattgcccgt ataggttgaa aaacaggatt
     4621 gatatgacga atctacccaa gttctccacc gcactgcttc atccgcgtta ttggttaacc
     4681 tggttgggta ttggcgtact ttggttggtc gtgcaattgc cctacccggt tatctaccgc
     4741 ctcggttgtg gattaggaaa actggcgtta cgttttatga aacgacgcgc aaaaattgtg
     4801 catcgcggaa atgagcgaac aagaacgccg taaaatggtg gtgaagaatt tcgaatccgt
     4861 tggcatgggc ctgatggaaa ccggcatggc gtggttctgg ccggaccgcc gaatcgcccg
     4921 ctggacggaa gtgatcggca tggaacacat tcgtgacgtg caggcgcaaa aacgcggcat
     4981 cctgttagtt ggcatccatt ttctgacact ggagctgggt gcgcggcagt ttggtatgca
     5041 ggaaccgggg attggcgttt accgcccgaa cgataatcca ctgattgact ggctacaaac
     5101 ctggggccgt ttgcgctcaa ataaatcgat gctcgaccgc aaagatttaa aaggcatgat
     5161 taaagccctg aaaaaaggcg aagtggtctg gtacgcaccg gatcatgatt acggcccgcg
     5221 ctcaagcgtt ttcgtcccgt tgtttgccgt tgagcaggct gcgaccacga ccggaacctg
     5281 gatgctggca cggatgtccg gcgcatgtct ggtgcccttc gttccacgcc gtaagccaga
     5341 tggcaaannn nnnnnnnctg ccgccagagt gttctccgcc gctggatgat gccgaaacta
     5401 ccgccgcgtg gatgaacaaa gtggtcgaaa aatgcatcat gatggcacca gagcagtata
     5461 tgtggttaca ccgtcgcttt aaaacacgcc cggaaggcgt tccttcacgc tattaaatct
     5521 tccatgctgg atgcttcata atggcatccg gcattaccac agaaaatccc cctgatttag
     5581 cgataaaagc tctctggatt gcgccccctg gaagtcgggc gcataattag tgtgcttatc
     5641 ttttcttctt atgttcaccg cgcctggcgc accaacagcg gattcctatg tcaccctgtg
     5701 aaaatgacac ccctataaac tggaaacgaa acctgatcg
//
LOCUS       DAWDRC010000214         5717 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6345_length_5717_cov_2.128400, whole genome shotgun sequence.
ACCESSION   DAWDRC010000214 DAWDRC010000000
VERSION     DAWDRC010000214.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6345_length_5717_cov_2.128400"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1871..1880
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3078..3087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3460..3469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1557 a         1395 c         1254 g         1481 t
ORIGIN      
        1 gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa gagagatatt gccctgaatg
       61 ggtagagagt ttattgactt cgctcaaact ttacggcgtt tttgtataca gacagccggg
      121 aaaattgctt ttgttacaac catttactac gatgcaacca taaagcaaca ccaccaataa
      181 gaacaactaa cagaatacaa aaaattgaaa atccgaattg ccatccaccg ccagggatcc
      241 caccaaggtt gacgccaaat aaccctgtca gaaacgtact gggtaaaaag accattgcca
      301 tcaacgacat tgtataggta cgacgagcta aattttcctg catcacctga gcgatttcat
      361 cagccatcac gccagtccgt gctatacagg cgtcgatttc gtcaagaccg cgcccaaggc
      421 gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct catccacggc atacgttcac
      481 tggcaagacg agcataaaca tcacgttgcg gtgccatata gcgacgcatc acaattaatt
      541 gtttgcgcag cagagccagg aatccacgcg gtggaatttg ctgatcgaga agattatctt
      601 cgaggtcgat aattttatcg tgcagctgct cgataaattc gctggaatga tcggtcaacg
      661 catcgcacac atccaccagc caccccccgc aatcggtcgg accagtgccc tcttccagat
      721 cgctcaccac atcgtccagc gccagcactt tgcgttgtcg ggtcgaaaca attaatcgcc
      781 catccatgta tacacgcatg gcgaccagct gatcggggcg ttcatcggtg ctgccgttta
      841 tacagcgcaa tgtaatcagc gtgccttcac cgagacggct gactcggggc cgtgtgctct
      901 cgcccgccag cgcatcacgt acgttattgg gaagcagcgg tgttgtcgcc agccattggg
      961 cgctatcatg gtgtacataa ttaaggtgga gccagcaggg atgcgcttca tcaatcacat
     1021 ctgtattttc cagcggttta acgccgcctc taccatccag catccaggca aatactgcat
     1081 ccgggacatt aacgtccgat cccttaatcg cttccacagt gcctccatca tcaacgcatt
     1141 attttgtagt ctagccttct ggccctgtta cgcaacatct catcacccca ttaccctgaa
     1201 atgattaata aaattctgtc taaattgaat acaaaaagca aaatgctttt ccgtatacaa
     1261 accgtgtgaa gtgttaaata gcgtctatca ttatcagaat tatctgatca tatgacgtgg
     1321 cttttttgct atcggatagc aaaaaaaatt tataaaaata atgggatctc aatgattaca
     1381 cacaacttca atactctgga cttactcacc agtcctgtct ggatcgtttc gccctttgag
     1441 gaacagttaa tttatgccaa cagcgaggcg cgactgttga tgcaagacct cacgtttagt
     1501 cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag aactgccgaa atacctctcc
     1561 gatctgcaaa atcaacacga tattatcgaa atcctcaccg ttcagcgcaa tgaagaggaa
     1621 acagcattga gctgtcgact tgttttgcga aagctgacag aagcagaacc ggtgattatt
     1681 ttcgaaggta tcgaagcgcc ggcaacactg ggtttaaaag ccagtcgctc ggcaaattat
     1741 cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga ctaactctgc accaatgttg
     1801 ttgattgacc cctcacgaga tggccaaatc gtcgatgcta acctcgccgc gctcaatttc
     1861 tatggttata nnnnnnnnnn gatgtgccag aaacatacct gggaaattaa tatgctcggg
     1921 cgtcgcgtca tgcctatcat gcatgaaatc tcgcatttac ccggtggtca taaacctttg
     1981 aattttattc ataaactggc ggatggttcg actcgtcatg tgcagaccta tgccgggccg
     2041 attgaaattt atggcgacaa gctcatgtta tgtattgtgc atgatattac tgagcaaaaa
     2101 cggctggagg agcagctgga acatgctgcc caccatgatg cgatgacagg attactgaat
     2161 cggcgacagt tttatcacat taccgagcca ggccaaatgc agcacctcgc tatcgctcag
     2221 gattacagct tgttgctcat cgacaccgat cgttttaaac acattaacga tctctatggg
     2281 cattctaaag gtgatgaggt gttatgcgcc cttgcccgca ctctcgaaag ttgcgctcgc
     2341 aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg tcttattgct accaagaacc
     2401 ccactggata ccgcgctttc gctggctgaa actatccgcg taagcgtggc aaaagtgagt
     2461 atttcgggct taccacgctt tactgtcagc attggtgtgg cgcatcacga aggaaatgaa
     2521 agcatcgatg aactgtttaa acgcgttgat gatgctttgt atcgagcgaa aaatgatgga
     2581 cgcaaccgcg tgctggcggc ataagccacg gatgcgtctc gtgatcaacg actgcgctta
     2641 gcgtggcgct cccagttttc ttgcttcgcc tgcgctgttt tacgtagtgc gacgtaacac
     2701 gccccgctgc cgccatgatg cggtagcgca gtgcaatatg cctgaacatc atcaaattcg
     2761 gtcagccagc gcgccacata gctgcggaca atattggcat gcgatttatc atcccgtcct
     2821 ttgccatgaa taatcagcac gttacgcaaa ccatccgcca gggcttgttg aatgaaactg
     2881 aacatcattt tgcggcactc ttccaccggc tggcgcaaaa gattcaggct cgcctgttgc
     2941 ggatatttac cactgcgcag cttatccagc accccatgtt gcaaaccttc ccgccgaaac
     3001 tccagcgact gacttagcgg gatgatgtcg agaaatccag tggtgaggaa attatcaagt
     3061 tgcagcgtgt cgatacgnnn nnnnnnngag gccagtggac atctgtagca cgtttcagcg
     3121 gctggacatc ttccatggcg tcaagaaaca gcgatttgtc gtcaaggttc atgtaacctc
     3181 ctatgcacct tcacacgtcg tgattcagac gggttaattg ttcatactta ctgcatcgtt
     3241 cccttcacag attgaaacaa tactctactt catacctgtg agtatctgcg caggcggagc
     3301 attttatact agcctgttag cagtatcgaa cagtttagcc cgagtttaat tcagcacagg
     3361 ctccgatcag gatgatttat ccatagcagg aaccaacaaa aatgcgataa ataacagacc
     3421 actgattagc agaaaggtat ccgaaaatgc catagtttgn nnnnnnnnnc ctccctcagc
     3481 atcaattgac tcaataattt ggttgaagcc agtatttccg atgtctggtc acttccactg
     3541 cgattaaaaa acaaagcact tctggaaatg aagtcattca ccatatgggg aacagaaacc
     3601 attttttcac ccattctcga aaaatgaaaa ttagttcgat tattgagaat agagccacac
     3661 agggcaatac caattgcgcc cccaagatta cgtgttaaat taaatacacc agaggctaac
     3721 ttcagccgtt cttttggaat tccccccagc gttaatgtta cgatgggggc catcgcaaac
     3781 tgctgcgaaa tccctctaat ctggcgacat ttgcacgata atcccgatca tcgagacgca
     3841 ggataatatc gccttttttt acactctgat tatcgcgcac ctctatagca gaaatataac
     3901 cagaaacttt acttgccact gtggtgatat taccaccaat gtaagcatca tccgtcgtct
     3961 gtataaatct tcctttgctt accccccaga taaaaaagac aacgccagcc aaaagaatac
     4021 cgatcgctac caccccaata agctgttttt tagaaattat catcactctt tacaccgtat
     4081 atatccggaa acttgcatac gtcatattta aaatttaaca tggcttattt gatgatttct
     4141 gacaaaatac ctactgtaaa tgacaatcac agtgcaaccc aaagcatcac cagggcatca
     4201 cattatcgga ctggattcgg aacatctgaa tgtaggtaca gttccctggc ataaacatct
     4261 ctatgcccag ttgctttacc cggctgaagg agtcgttcgt gtatgggctg gagaaagcgt
     4321 ctggctagtg catgccagca gcgcgctttg gctgccccca caaatgccgc ataagtttgt
     4381 tgcgacaggc aacgtacttt taaaaaccgt actggtttca gaagcggaaa gtgaaacgct
     4441 gggaaaagtt tgcttcatga cgggtatttc acccctgtta agagaattac taattgcaat
     4501 taaccaactt ccaccgtcgc aaagtaccac agataaacaa cagttacgtt tttcagccct
     4561 cgagacactg atacttcagg aaataaaaat gggggtgaaa atgtcacttg aactcccttg
     4621 gccaaatgat gagcggctac agcagctttg tgagaatttg ctaaacaacc aagggtatct
     4681 gccaacactg gataatctag cggataaaac taacgtcagt agccgtacac tcatgcgctt
     4741 atttgttaaa gagacgggat taacatttcg ccactgggtt cagcaaatgc acgttatttc
     4801 tgcagtgacg cttcttgatg atggatattc gttgaccaaa attgcccatc gtctgggcta
     4861 tgcctcagct gaatcgttcg gcaatatgtt taagcgtcga acgggctact cgcccggtaa
     4921 atttactcgt cgattaacga tgcatgatta tgcaatcaca aggcaaatga tttaacttat
     4981 attttcgtga aatctgtcac tgaagaaaat tggcaacgga tgggtaaaac cgttataaca
     5041 ctgtcaccgg tcaccaggac cccaggccgg atttaagacg aggatgcact gctgtgtgta
     5101 ctgtagagtc tggcggatgt cgacaggctc tattttttta ttcatttttt agccctgtaa
     5161 ataaccacat tctcgccggg attggggaat gagtgacgag atcagtggtg tctggcggct
     5221 atcacgccac agaatcaaat aacggatagc ctagggtaaa atttcgctga ctggaatttg
     5281 atggcttgcc ctcatttctt caggcagaaa aatcaaaaaa tcgctttatg cttaaatact
     5341 tcgacaaacc agacgctaaa cctggtactt actttttgtt agtattaatc ttttcggtgg
     5401 tatggctgct gatgttgctg gcgtggtatc ttgttggctt gccgataggt ccgggtattt
     5461 acccacgttt gtcttaagag agaacggatg ctgagattac ttgaagaaaa aattgccacg
     5521 ccactgggtc cactgtgggt gatttgcgat gagcaatttc gcctgcgggc ggttgaatgg
     5581 gaagagtaca gcgaacgcat ggtgcagctg ctggacatcc attatcgcaa agaaagctat
     5641 gagcgcattt ctgccaccaa cccaggtggt ttaagcgaca agcttcgtga atattttgcc
     5701 ggtaatctta gcattat
//
LOCUS       DAWDRC010000215         5700 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6366_length_5700_cov_2.420195, whole genome shotgun sequence.
ACCESSION   DAWDRC010000215 DAWDRC010000000
VERSION     DAWDRC010000215.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6366_length_5700_cov_2.420195"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    5303..5312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1477 a         1334 c         1395 g         1484 t
ORIGIN      
        1 taaaggcatt tcctgaaaaa taaaacaatc aattaacaac gaagaataac tctgcgtata
       61 tttgcgtatt aatgcattat cgtattttgt ttctttatca tttatcagaa ccgcatcaaa
      121 tattatcaac ttcgattatt aatatcaccg ccggtgtgaa agtcgtcaca ttttctggaa
      181 gaaataaact gaatcattgc atctgaaaat tactgaacag acctgactac aagcatttaa
      241 tttgtttgca gccatctcct ctttattttt gaaaaagtgt taaaggaata atgatggaaa
      301 agcattatgt tggttctgaa attggacaac tacgcagcgt tatgttgcat cgcccaaatc
      361 tcagtctgaa acgtctaaca ccatccaatt gccaggagtt gttatttgac gatgtattgt
      421 ccgttgagcg ggcaggtgaa gagcatgata ttttcgccaa tacgctgcgc cagcaaggga
      481 tagaagttct gttactcact gatttactaa cacaaacatt ggatatcccg gaagccaaaa
      541 gctggttact ggaaacacaa atatcagatt accgactcgg ccctacgttt gctactgacg
      601 tccgcacctg gcttgcagag atgtcacacc gggaccttgc tcgtcattta agtggtgggc
      661 tgacttatag tgaaattccc gcctcaatta aaaatatggt ggttgatacc cacgatatta
      721 atgatttcat tatgaagccg ttaccgaatc atttatttac gcgtgatacc tcatgctgga
      781 tttataacgg tgtatcgatt aatccaatgg caaaacctgc tcgccaacgt gaaaccaata
      841 atctacgcgc tatttatcgc tggcatccac aatttgctgg cggcgaattt attaaatatt
      901 tcggcgacga gaatattaat tatgaccacg ccacgttaga aggtggtgat gtgctggtga
      961 ttggtcgggg ggccgtgttg atcggcatgt ctgaacgcac cacgccacaa ggagtcgagt
     1021 ttcttgctca ggcattgttt aaacatcgtc aggctgaacg ggtaatcgct gttgagctgc
     1081 ctaaacaccg ctcctgtatg catcttgaca ccgttatgac tcatatcgat atcgacacct
     1141 tctcggttta tcccgaagtt gtgcgcccgg atgttaattg ctggacgcta acacctgacg
     1201 gtcacggcgg cctgaaacgt actcaagaaa gcacactcct ccatgctatc gaaaaagccc
     1261 tcggtattga ccaggtacgt ttaatcacca cggggggtga tgcctttgag gcagaacgcg
     1321 aacagtggaa tgacgctaac aatgtgctca cgcttcggcc gggagtggta gtgggctacg
     1381 agcgcaacat ctggactaac gaaaaatatg acaaagcagg catcaccgtt ttacccatcc
     1441 ccggtgatga attaggccgc ggacgtggcg gcgcacgttg catgagttgc cctttgcatc
     1501 gtgacggtat ctaaggaaga gatcatggaa aacaaaccaa cactggttat cgctctcggc
     1561 ggtaatgccc tgcttaaacg aggcgaacca ctcgaagcag aaatccaacg taaaaatatc
     1621 gatcttgcgg ctaaaactat cgcccaactg actcaacact ggcgggttgt actggttcac
     1681 gggaatggtc cacaagttgg acttctggca cttcaaaata gcgcctatgc ccatgtgacc
     1741 ccttatccac tggacgttct tggggccgaa agccagggga tgatcggcta catgctacaa
     1801 caggcgctga aaaatcaact accacaacgt gaaatcagcg tgctgctgac gcaggttgaa
     1861 gtggatgcca acgatcccgc cttcagtaac ccaactaagt acatcggccc aatttatgac
     1921 cacgctcaga ctcaggtact acaggcagaa aaaggctggg ttttcaaagc agatggtcac
     1981 tctttccgcc gggtcgtacc ttctccacaa cctaaacgca tcgtcgagcg ggatgccatc
     2041 cagacactga tcgcccacga tcatctggtg atctgtaatg gtggcggtgg tgttccggtg
     2101 gttgaaaaag ccgatggata ccatgggatt gaagcggtta tcgacaaaga tctttcagcc
     2161 gcgctactgg caagtcagat ccatgctgac gccttgttga tcctcactga tgcggacgca
     2221 gtatatctcg actggggcaa gccaacccaa cgcccgctgg cccaggtcac gccggaatta
     2281 ctcaacgaaa tgcagtttga tgccgggtct atggggccga aagtcaccgc ctgcgcaaag
     2341 tttgttagtc agtgccgggg cattgcaggg atcggctctc ttgcagatgg tccggaaatt
     2401 ctcgccggag acaaaggcac tcttattcgc cttgataccc ccataaccac gcttgatcca
     2461 tttttataag gacatcccca tggctacttc actgaaaaac cgtaattttc ttaagctgct
     2521 tgattacacc ccagcagaga ttcaatacct gattgatttg gctatcaatc ttaaagccgc
     2581 caagaaatct ggaaacgaaa aacaaacgct ggtcggtaaa aacattgcgt tgatttttga
     2641 aaaatcctct acccgtaccc gctgtgcctt tgaagtcgcg gcctttgatc aaggtgcgca
     2701 ggtgacttac atcggcccca gcggctcaca aatcggccat aaagaatcaa tgaaagatac
     2761 cgctcgcgta ctcggacgca tgtatgacgg tattgagtat cgcggttatg gtcagaatat
     2821 cgttgaagaa ttaggtgagt tcgctggtgt tccggtatgg aacggactga ccaatgaatt
     2881 tcacccgaca cagatcctcg cggatttaat gaccatgctg gaacatgcgc caggcaaaac
     2941 gctgccagag ctgagctttg cgtatcttgg tgatgcccga aataacatgg ggaactcgct
     3001 gatggttggt gctgcaaaaa tggggatgga tatccgtctg gtggcaccta aatctttctg
     3061 gccagatgaa gcgctggtaa cacaatgccg tgagatcgcc agcgtgactg gggcgagaat
     3121 caccctgaca gaggatgtgg aagaaggcgt atacgacgtt gattttctct atacggatgt
     3181 ttgggtttcg atgggagagc caaaagaagc ctgggcagaa cgagtgagtc tgatggcacc
     3241 ttatcagatc aatcaacagg tgattactgc cacgcgcaac cctgaagtta aatttatgca
     3301 ctgcctgccc gccttccata atgagcacac cacagtagga cgcgagatcg agatggccta
     3361 cggcctcaaa ggactggaag ttactgaaga ggtttttgaa tcggcccact ctattgtttt
     3421 cgatgaagca gaaaaccgga tgcacaccat taaagcggtg atggtagcaa ctctcggcga
     3481 ctaaccctga cgggcatgcg tcgacgagtg gcgtatgccc gtgattatgg agatcacatt
     3541 atgggtaaat tcaaatttcc caccgcttat accatcttgt tcgtgctgat tgcgttagtt
     3601 gcggcaatga cctggattgt acctgcggga aaatatcaga tggtgacaaa tgccgcactg
     3661 ggaaaggaag ttcctgttgc aggcacttac gcacctgttg aggcacaacc acagggtatt
     3721 acggctgtcc tgttagcgcc catcgacggc ttgtacaacc atcaaaccta cactgcggga
     3781 gcgattgatg tcgcgctgtt tgtcctgatc atcggtggat ttttgggagt agtaaataaa
     3841 accggggcaa tagatgccgg tatcgaacga gtggcaactc gccttaatgg tcgggaagag
     3901 tggatgattc cgatcctgat ggcgctcttc gctgctggcg gtactattta tgggattttg
     3961 ctgcgtgtta ttttgctgtt agtaggctac gtgatttgtg tttactgggt tatgcgttat
     4021 gcccgcaaag ttcgcaacag tccggaaagc tctatcgtgg cggataagat ggctgaaaac
     4081 caggcgcatt ttctgggaaa tcgttcagaa atgatgctgg aatttacccc cacgcgcaaa
     4141 gcgatcctga tactttttgc tgcatccttt gcctttatga tttacggcgt agcggtactg
     4201 ggttggtgga tggcggaaat ctctgctgtc tttctggcag ctgcggtcat cgtgggtgtc
     4261 attgcccgca tgggcgaaga aaccttcacc agtaccttta ttgacggcgc gcgggatctc
     4321 cttggtgtgg cactgattat cggcatcgcc cgcggtattg tggtcgtgat ggataacggc
     4381 atgattacgc acacaattct gcacagtgct gagaatctgg tttcagggct ttccaccact
     4441 gttttcatca acgtgaccta ttggctggaa gtattgctct cttttctggt tccctcatct
     4501 tccggccttg cagtgttaac gatgccaata atggctccac tcgctgattt tgctcatgta
     4561 caacgcgatc tggtggtgac cgcttatcag tctgcttccg gggtggttaa tttgattacg
     4621 ccaacctctg cggtagtgat gggcggtctg gcgattgccc gtgtaccctg ggtccgttat
     4681 ctgagatggg tagcaccgct actgctgatt ctcacgatgt tgaatatgat tgtgctgagt
     4741 atcgccgcga tgatttaacg cgacaggccg cttatcaaag cggccttcaa atctggtgta
     4801 gtgataaatg aaccactctc acattataaa atgtgaaaaa tatcatcagg taataataaa
     4861 taaaaggtgc attatgaagg aatacgatga ttactccgcc aaagaaaaga agcaactggc
     4921 ggtctgtcag cgcctgatta ccgaaaaaag ttatctttca caggaggaaa tccgccgcga
     4981 tttgcaaaac catggctttg acagtatcag tcagtcaacc gtttcacgac tactgaaatt
     5041 gctgggagtc ataaaaatta gaaatacaaa aggacaaaaa atttattcgg tgaatcctca
     5101 attacttccc acacctgacg caggtcgatc cgtcgctgaa atggtcctga gtgtcgagca
     5161 taatggtgaa tttatcctta tccataccgt cgccggatat ggtcgggcag tagcgcgaat
     5221 ccttgatttc catgctttgc cagaaattct tggcgtgatt gctggcagca atatcgtttg
     5281 ggtcgcacca cgtgtggtaa agnnnnnnnn nncgcacagc cctggtgcac aagcaaatta
     5341 attacttatt aaaattgaat atatattcat gataaccgtt tgccttgctt tataactgca
     5401 tcatcttctt gatcctctct cagagctgag tataatgccg gacaatttgc cgggaggatg
     5461 tatggttcag tgtgttcgac attttgtctt accgcgtctg aaaaaagacg ctggcctgcc
     5521 gtttttcttc ccgttgatca cccattccca gcccctcaat cgaggggctt ttttttgccc
     5581 aggcgtcagg agataaaaga tggctaatcc gctatatcag aaacatatca tttccataaa
     5641 cgaccttagt cgcgatgacc ttaatctggt gctggcgaca gcggcgaaac tgaaagcgaa
//
LOCUS       DAWDRC010000216         5667 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6408_length_5667_cov_2.871169, whole genome shotgun sequence.
ACCESSION   DAWDRC010000216 DAWDRC010000000
VERSION     DAWDRC010000216.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6408_length_5667_cov_2.871169"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3102..3111
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1494 a         1447 c         1376 g         1340 t
ORIGIN      
        1 caccgctaat ggtcagcatg gagcaaaatc tggcggagtg gatgcaaatg agcaacctgc
       61 tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagctg gcagcagaat
      121 caccgctaat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc
      181 aggcgaccat caaattacgt aactgggatt acgattcctt agatgccatt actcgtggtg
      241 aagtggatat tggtttttcc ggtcgcgaaa gtcatccccg ttcgcgggag ctgttaagct
      301 cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtgccctgc gtctggttac
      361 gcaaagatca tccggcactg catgaagcat ggaatctgga caccttttta tgttatccgc
      421 atatcagcat ttgctgggaa cagagcgata cctgggcgct ggacaatgtg ttacaggagc
      481 tggggcgcga acgcacgatt gctatgagcc tgccggaatt cgagcagtca ctgtttatgg
      541 cagcacagcc cgacaatctg ctactggcga ccgcgccgcg ctactgccag tactacaatc
      601 aactccacca actgccgttg gttgctcttc ctctcccgtt tgatgaaagc cagcaaaaaa
      661 agctggaagt cccttttacc cttctgtggc ataaacggaa cagccgtaat ccgaagatcg
      721 tctggttacg ggaaaccatt aaaaatcttt acgcgtcgat ggcataaccg aatcgtatga
      781 aacgcgaccc aatttcacaa tgaaatgtaa aaaagttgta ataaacttat ctgaatcgaa
      841 cttttagccg ctttagtctg tccatcattc cagtaaatga ttactcttgt attcataatg
      901 gaccattaag catggagcga aaaatggcga ctcactttgc ccgagggatt ttaacggaag
      961 gacatctgat ttctgttcgt ctcccctccc tgggtcatca agaagcccga aacattcccc
     1021 ctcatcgtca aagccgtttt ctggcgtcca gaggtttact cgcagaactg atgttcatgc
     1081 tgtatggcat tggcgaattg ccggaaatcg tcaccctgcc gaaaggaaaa ccggttttca
     1141 gcgataaaaa tttgccttcg ttttctattt cctatgccgg gaatatggtc ggcgtggcgt
     1201 taacaaccga aggtgaatgt ggcctcgata tggaactaca gcgtgcgaca cgcgggtttc
     1261 atagcccaca cgcgcccgat aaccacacct tttccagcaa tgaatcgcta tggatcagta
     1321 aacaaaacga tcctaacgaa gcgcgggcgc agctcatcac gctgcgccgg agcgtgctaa
     1381 aactaaccgg tgatgttttg aatgacgatc cgcgcgatct gcaactactg cccattgccg
     1441 gacgcctgaa atgtgctcat gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc
     1501 tggtgtggtc cgtggcggtc acgcccacga ttgaaaagct cagtgtctgg gagttagatg
     1561 gcaaacacgg ctggaaaagc ctgcccgata ttcacagccg cgccaacaac ccgaccagcc
     1621 ggatgatgcg ttttgcccag ctctctaccg tgaaggcttt ttcgccaaat tgatagacaa
     1681 ccacaggagt catcatgtct gaaaaattgc aggtggttac gttactgggg agcctgcgca
     1741 aaggctcatt taatggcatg gttgcacgta ccctgccgaa aattgctccg gcgagcatgg
     1801 aaatcaatgc gttaccttcc attgccgaca ttcccctgta tgacgctgac gtacagcagg
     1861 aagacggttt tccagcaaca gttgaagctc tggcggaaca gatccgtcag gctgacggtg
     1921 tggggatcgt cacgccggaa tataactact cggtaccggg tgggctgaaa aatgccatcg
     1981 actggctttc ccgcctgccg gatcaaccgc tggcaggtaa accggtattg attcagacca
     2041 gctcaatggg cgtgattggc ggcgcgcgct gtcagtatca cctgcgccag attctggttt
     2101 tcctcgatgc aatggtgatg aacaagccag aatttatggg cggcgtgatt cagaacaaag
     2161 ttgatccgca aaccggggaa gtgattgatc agagtacgct ggaccacctg accgggcaat
     2221 tgaccgcatt tggtgagttt attcagcgag ttaagatcta aataaaaacc cgccagcaat
     2281 catgcatggc gggtttttaa cgcgctatcg attttagtga gcgtcgataa agacaatctt
     2341 caggataaac agcagcgcaa cgatgattac gcacgggcta aggtcacgca gacgtccggt
     2401 accaatcttc atcacgcagt aggagataaa gcccagcgcg ataccttcag taatcgagaa
     2461 gctgaacggc atcatcacag cggtaataaa cgccggaaca gattcagtaa gatcctgcca
     2521 gttcacgcga gcaagacttg aggtcatcaa cacgccaacg taaatcagcg caccagctgc
     2581 agcgtagcct ggcaccatcc ccgccagcgg cgacaggaag ataaccagca ggaacagcag
     2641 accaacaacc actgccgtca gaccggtacg accgccaacg gaaacgccgg aagaagattc
     2701 aatatacgcc gtaacagaag aagtaccgat aaacgaaccg gtcaccgaag agatactgtc
     2761 gacatacagc gcctgcttca tgcgcgggaa tttacctttc tcatcagcca gacctgcttt
     2821 atcggtcacg ccaatcagcg taccggagga gtcaaacaag ttgaccagca tgaaagagaa
     2881 aatcacccct gccagcccaa ggttaaacga cccggctaaa tctacatgac ccacgacact
     2941 cattacgctc ggcggcgcag aaacgatgcc attatagtgg acatcaccca gcatccagcc
     3001 cagcagcgtt gtcaccacga tggaaaccag caccgccgcg tgaatattgc gcgaggccag
     3061 aatggcgatg atgaagaagc cgaggatccc cagcagcacg cnnnnnnnnn nctcaccagc
     3121 gtttccgggt tagcgacaat cacacctgcg tttttcagcc ccatcatgcc aataaacaga
     3181 ccgataccac tggtgatgcc cacacgtaga cttaccggga tgttggctat catccagtag
     3241 cgaacgcgga aaatcgtcag taacagcaga ccaatcgcgc cccagaagat tgcgcccatc
     3301 ccgacttgcc acggcaagcc catcgcctgt acaacgacaa aagcgaagaa cgcattcagg
     3361 cccattgccg gtgccagtgc aactggcagg ttagcaaaca gccccatcat aatactgccg
     3421 aatgcagcaa tcagacaggt cgtgacgaag acggcgctgg tatccatgcc agcaacgcca
     3481 agaatttgcg ggttaacaaa aacgatgtaa accatcgtca ggaaggtggt aaaaccggcg
     3541 atcacttcgg tccgtgccgt cgtgccatgt tcgcgcagtt taaacacgcg ttccagcatc
     3601 ccctggccag aagcctgggt tgtgtgttgt tgactcatta tctatttccg aacaaggagg
     3661 gaaaatccgt cgctatcgta taccaaaatg agacaatatg gacggctgaa gataactttt
     3721 tttcattgta tttgcttata cggcaacgat tgcgtcatgc aaattggcat gacgaaaacg
     3781 ttaaactaac gataaaggaa agacatgtcc cagatagaag cggtattttt cgactgcgac
     3841 ggtacgctgg tcgatagcga ggtcatttgc tctcgcgcct atgtcacgat gttcagggaa
     3901 tttggcattc acgttgacct ggaagagatt tttacccgtt ttaaaggcgt aaaactctac
     3961 gaaatcatcg acatcatcag taaagaacag ggcgtgacga tggtccaggc cgatgccgaa
     4021 cacgtttacc gcgcagaagt agcacgtctg tttgataccg aacttgagga catcgcagga
     4081 gcgaacgaat tgctggcaag cattaaagtg ccgatgtgcg tcgtctctaa cggtccggtc
     4141 agcaaaatgc agcattcgct tggtaagctc aacatgctgc actacttccc ggaaaaactg
     4201 ttcagcggct atgatatcca gcgttggaag cccgatccgg cattaatgtt ccatgcggca
     4261 aaagcgatga atgtgaacgt tgaaaactgc attctggtcg atgactccag cgcaggtgcg
     4321 caatcaggga tagatgccgg aatggaagtg ttttacttct gcgctgaccc gcacaataaa
     4381 ccgatcgttc acccgaaagt caccaccttt acccatcttt cgcaattacc ggaactgtgg
     4441 aaggcgcgtg gttgggatat tacggcatag ttattcacac tcccttcact tactccactt
     4501 aaattggcac gcaaaggtaa gtaaagggag tttgatatgt ctgtttcacg tcgggtaatt
     4561 catcacggac tgtattttgc ggttttaggg ccgttaattg gtgttctgtt tcttgtcctc
     4621 tacatattct tcgcaaaaga accgctgatt cttttggtaa taatacaggt acttccattg
     4681 tttctcttat tgtcgataac tacgggagct attcctgcaa tgttaactgg cgtaatggtc
     4741 gcctgtctgc cggaaaagat cgggtcacag aaacgttatc gttgtctggt tggcggcata
     4801 ggtggagtcg ttatcaccga gatctattgc gctgttatcg tacatattaa ggacatggct
     4861 tcctcagcgt tgtttgaaaa cattctttct ggtgaaaatc tcgttgtccg catcatccca
     4921 gcattgctgg caggcgtagt gatgagcaga atcattacac gtctaccggg attggatatt
     4981 tcatgtcctg aaacagactc tttaagctaa gcggaatgtt ttatctattt gtcattaaat
     5041 gttggagaac atttttcagt gcctgcgctt tacctgaacc actgacaatt gtcagcaagt
     5101 ttttcgccgc catgatgctt ttcggcccca tcgtcacgta gctgtcaggc actaatgaaa
     5161 agtcgccacc caactcgcca tgcgccacaa tatcaaccat ctctccctgg atcgggaact
     5221 ccacggtttg ctcatggaaa tgggtcgtat ttggcaaatt gccacaaaaa tgaccatctg
     5281 cacctaatcc cagcaccacc aaatccaggc cgccttcacg cgccagtttc tgatcatgct
     5341 cgcggtagtt atcaatagtg agcttctgga tattctcttc tttgatcccc gcaggggtga
     5401 aaaacagatt acgcagattg gtaatcgtta cgccttctcc ctctttgccg cgaaatggaa
     5461 tttcatcgaa attatagaaa tagcagttat cgtaccaggg cttaccttta accagagtga
     5521 tgagatattc gtacatgcct ttgggcgtgc tcccggcggt aattgccagg ttaacacgac
     5581 gcgtcttcga catataacca agcagatggt gtgccgcgac acggctcatt tcctggtaat
     5641 cttcggtaat gattaatttc atcttaa
//
LOCUS       DAWDRC010000217         5665 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6412_length_5665_cov_2.140285, whole genome shotgun sequence.
ACCESSION   DAWDRC010000217 DAWDRC010000000
VERSION     DAWDRC010000217.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6412_length_5665_cov_2.140285"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    874..883
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1388..1397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1286 a         1442 c         1500 g         1417 t
ORIGIN      
        1 ccgacaatcc agtttggatg cgggttttta ccaccaaaga ccgcgtggat tttgacaatt
       61 tcacgctgga aatcgagagc ttcgagatag tgggcaaagc ccatcaggtt ggcttctggc
      121 ggcagtttgt actgcgggtg cccccagtag ccattgcgga agatccccaa ctgcccgcct
      181 tcaacgaatt tcttcaggcg gttttgtacg tcgaagaaat agccagggga tgatttcggc
      241 catgaggaga ggctttgcgc cagttcggag gttttccgcg ggtcggcttt cagcgcatct
      301 aacacatcga tccagtccat cccggcaagc tgatagaagt gcaccagatg atcgtggcac
      361 cagagcgttg ccagcataat gttgcggatg atattggcgt tatccggcac tttaataccg
      421 atggcatctt cgatggcgta aaccgaagcc agggcgtgta cgccagtaca gacgccgcag
      481 atacgttcaa cgaacgccca cgcatcgcgc gggtcacgcc cttgcaggat gatctccagc
      541 ccgcgaaaca tggtgccgca ggagacggca ttggtgatca cattctgatc gttaatattc
      601 acttcgcagc gcatgtggcc ttcaatgcgc gtaatcgggt cgaccaccag gcggcgtccg
      661 gcattgttga tggtgtatcc ctgagtttcg tactgagtgc tcatgcctgt ttatcctcat
      721 tgcctggctg atgttcggtt tctgtaggtt gctggttatg acgtctgcgc tggtcaacgg
      781 cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac gccaagcgcg gttaaaccga
      841 cggtgtcggc ggtggaatgg gtacccattt gtgnnnnnnn nnnccacgcg gctgtagaac
      901 gaaccgcgat cccagaaacc attttccgca caacccaggc agccgtgacc tgactggatc
      961 gggaaggaaa cgccatcgtt ccagcgtgtg gaggaacagg cgttataggt ggtaggccct
     1021 ttgcagccca ttttgtacag gcagtaacct ttgcgggcag cgtcatcatc ccaactctgc
     1081 acgaactctc cggcgtcgaa gtgagcgcgg cgatagcatt tatcgtggat tcgctgacca
     1141 tagaacatca acggacggcc cattctgtcg acatctggca agcgatcaaa ggtcaccatg
     1201 taagtaatga tggcgctcat cacatccggg atcggcgggc agccaggtac tttgataata
     1261 ggtttgtcgg tgatgacttt gtcgataggc gttgcctgcg tcggattggg tcgcgcggcc
     1321 tgcacgcagc cccaggacgc gcaggttccc caggcgataa tcgcactggc tccggcagcg
     1381 gcacgttnnn nnnnnnngca cgcttgagtt tctcaataaa cggtcgaccg ctgctgatac
     1441 agaacatccc ctgctcgccc agcggcggat taccttcaac ggcgaggata tatttgccat
     1501 tgtattgcgc gatgatgtct tcaaagactt cttccgcctg ggttccggcg gcagccatca
     1561 aagtatcgtc gtaatcgagg gaaatcaggg aaaggatgac gtccttcgcc agtgggtgag
     1621 cggagcggat aaaagattcg gtacagcagg tgcattccag accgtggatc cataccaccg
     1681 gaatgcgcgg tttattctcc agcgcccagg caatctttgg tgccattccc gcgcctaatc
     1741 ccagcgacgt ggcagccaga ctacaatatt tgagaaagct gcgccgggta acgccctgac
     1801 gccgcatggc ctggtaaaat gtttcctcgt tattcatatc gcacgtctct tctccttgcg
     1861 acaccggcag gacaccgggc gcacatggtc ggcaagcgac gtccctgtag caaggcaaac
     1921 catcgcttca cgtgagatgc tgaaaacgaa agctcatcct tctgcactgg cgcacgtcgc
     1981 cagaaagtat tgttaataaa gcgtagtgaa acttttgcac aaaacaatac aaactgtgtg
     2041 gatttatctt ttagcgataa aaatggacct atttttcttt ttgccgggcg gtgaggatgt
     2101 ttagccggtt gctaaacgag taaaagagaa ggaattcatg gtgatgaaat gatcaatctg
     2161 ctgttatttc acgctatcaa tgcacttttg gctgtttttc aggcaaacaa acaaattagg
     2221 ggtttacacg ccgcatcggg atgtttatag tgcgcgtcat tccggaagtg tggccgagcg
     2281 gttgaaggca ccggtcttga aaaccggcga cccgaaaggg ttccagagtt cgaatctctg
     2341 cgcttccgcc aaataagata aggggttagc taaatgctaa cccctttttc ttttgcctgt
     2401 cgaaattctc agggcgttat atcactgcct aatgacctga gactccgctg tgaaacttgt
     2461 ttcagatcaa atgcgtaaag atgagtaaaa cttctgtgta ccttcccaca ttatcattat
     2521 gctgcttaat taattacatc tgtcatagag agtgactcaa tggatcgtat agttagttct
     2581 tcacatgacc gtacatcgct gcttagcacc cataaggtgc tgcgtaatac ctattttctg
     2641 ctgagcctga cgctggcctt ttcggcgatt accgcaactg ccagtacggt gctgatgctg
     2701 ccatctccgg gtctgattct gacgctggtg ggtatgtatg gtttgatgtt cctgacctac
     2761 aaaacggcga ataagccgac cgggattatc tccgcattcg cctttaccgg ttttctgggt
     2821 tatatcctcg gaccaattct gaacacctat ctgtctgccg gaatgggtga cgtaatcgct
     2881 atggcactgg gcggaacggc gttagtgttc ttctgctgct ctgcatatgt gctgaccacc
     2941 cgcaaagata tgtcattcct tggcggtatg ctgatggcgg gtattgtggt ggtgctgatt
     3001 ggtatggttg ctaatatctt cctgcaactg cctgctctgc atctggcgat cagcgcggtc
     3061 ttcattctga tctcctctgg cgctattttg tttgaaacca gcaacatcat tcatggcggt
     3121 gaaacgaact atattcgtgc cacggttagc ctgtatgttt cgctgtacaa catcttcgtc
     3181 agcctgctga gcattctggg cttcgccagc cgcgattaat ctcgcccagg gttacttacc
     3241 gagcctcgct ttatgcgggg ctttgttttt tgttacactg ccgccagtct gattaaggta
     3301 gtaaagaatt atgctgatct tcgaaggtaa agagatagaa acggataccg aaggctatct
     3361 caaagaaagc agccagtgga gtgagccgct ggcggtggtg attgcagaga acgaagggat
     3421 tgagctgtcg ccagaacact gggaagtggt gcgttttgtg cgtgatttct atctggaatt
     3481 caatacttct ccggcgattc gtatgctggt aaaagcgatg gcgaataaat ttggcgaaga
     3541 aaaaggtaac agccgctatc tgtaccgact gttcccgaaa ggtccggcaa agcaagccac
     3601 caaaattgct ggcctgccta aaccggtaaa atgcatttaa cggatacgaa aatccgttaa
     3661 ctccccggag ggatgatgcg gctcgctaag cacccgttcc acacgcgctg aacgcgggcc
     3721 gccacttttt aaccactgca ttaatttttc cacctgccct tcgtcaccgc aggcaaccac
     3781 ttccacgctg ccatcgtcaa gatttttggc gtacccggtt agtcccagct ttttcgcttc
     3841 gtactgcgtg gtgtagcgga atcctacgcc ctgaactcgc ccgtaaaccc aggcaattat
     3901 gcagactttc gacattgctg ctctccttat ccttaatgga cgcgttgcaa atccatggaa
     3961 aactccggac aatgacgccc atttctttgc atcgacagaa aagtaaatta tgagtgtacg
     4021 tttagtgtta gccaaagggc gcgaaaaatc attacttcgt cgccatccgt gggtcatttc
     4081 cggggccgtt gcccgcatgg aaggtaaagc cagcctcggt gaaaccatcg atattgttga
     4141 tcatcaggga aaatggttag cacgcggcgc ttattcgcca gcttcgcaaa tccgggcgcg
     4201 cgtctggacg tttgacccgt cagagtctat cgacattgct ttttttaccc gccgtttgca
     4261 gcaagcacaa aaatggcgtg actggctggc gcaaaaagat ggcctcgaca gctatcgttt
     4321 aatcgccggt gaatctgatg gcctgccggg tattactatc gatcgtttcg gtaattttct
     4381 ggtgctgcaa ctgctgagtg ctggcgcaga gtatcagcgc gcggcattaa ttagcgccct
     4441 gcaaacgctg tacccggaat gtgctattta cgatcgcagc gacgtcgcgg tacgtaaaaa
     4501 agaagggatg gagctgactc agggccccat caccggcgag ttgccgcctg ccctgctgcc
     4561 gattgaagaa cacggcatga aactgctggt ggatattcag cacggacaca aaacgggcta
     4621 ctacctggac cagcgtgaca gccgcctggc tacccgccgc tacgtcgaaa ataaacgcgt
     4681 acttaactgt ttttcctata ccggtggttt cgccgtatcg gcattgatgg gcggttgcag
     4741 ccaggttgtc agcgttgata cctcccacga agcgctggat attgcacggc agaacgttga
     4801 gctgaacaaa ctggatctga gcaaggcaga gtttgtccgt gatgatgtct ttaaattgct
     4861 gcgtacttat cgcgatcgcg gtgaaaaatt tgatgttatc gtgatggacc cgccgaagtt
     4921 tgttgagaat aaaagccagt tgatgggcgc gtgtcgtggt tataaagaca tcaacatgct
     4981 ggcgattcag ttgctgaatg aaggcggtat tctcctgact ttctcctgtt ccggtctgat
     5041 gaccagcgat ttatttcaga aaatcatcgc ggatgccgca attgatgccg gtcgtgatgt
     5101 acaatttata gagcagttcc gtcaggcagc cgatcatccg gtgatcgcta cctacccgga
     5161 agggctatat ctgaaagggt ttgcctgtcg cgtcatgtaa cttgaaatgt ggaattttac
     5221 ccttatatca aaggtaaaga tttcccggga ggtgactatg attgccagca aattcggtat
     5281 cggccagcag gtccgccatt ccctgttagg ttatctcgga gtggtcgtgg atatcgaccc
     5341 ggtttattcg ctctctgaac cgtcgcctga tgagctggcg gtgaatgacg agcttcgcgc
     5401 cgctccgtgg tatcacgtgg tgatggagga tgataacggc ctaccggttc atacctacct
     5461 ggctgaagcc cagctaagta gcgaattgca ggatgaacat cctgaacagc catccatgga
     5521 tgagctggcg cagaccatcc gcaaacaact ccaggccccg cgtctgcgta actaatccct
     5581 gaaccaatcc ccggaaggtt tttgccttcc ggggattttt tatttggcca ggcccaggcg
     5641 agggatctca atcgccgggc accga
//
LOCUS       DAWDRC010000218         5661 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6418_length_5661_cov_1.977524, whole genome shotgun sequence.
ACCESSION   DAWDRC010000218 DAWDRC010000000
VERSION     DAWDRC010000218.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6418_length_5661_cov_1.977524"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3762..3771
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1428 a         1347 c         1395 g         1481 t
ORIGIN      
        1 gtattattat tgtgatgagt tgattgatat tgatgcaata tctcttgagt tatatatttc
       61 cggttatcta ttgtggaaat ttaatttaag tgcaggaatt atattttcgc cggattttat
      121 tcggattatc ctgcttatcc tcgtgctgtt tctcacgtag tctataattt cctttttaag
      181 cccacaggag agcaacaatg acaatccata agaaaggtca ggcacactgg gaaggcgata
      241 tcaaacgcgg gaagggaaca gtatctactg agagcggcgt gctgaaccaa cagccgtatg
      301 gatttaacac gcgttttgaa ggcgaaaaag gaaccaaccc tgaagaactg attggcgcag
      361 cacatgccgc atgtttctca atggcgcttt cattaatgct gggggaagcg ggattcacgc
      421 caacatcgat tgataccacc gccgatgtct cgctggataa agtggatgcc ggttttgcga
      481 ttactaaaat cgcactgaag agcgaagttg cggtgccggg tattgatgcc tccacctttg
      541 acggcattat ccagaaagca aaagcaggat gcccggtttc tcaggtactg aaagcggaaa
      601 ttacgctgga ttaccagttg aaatcgtaag cgttgccgga tgacgcgtca ggcgcgtgaa
      661 tgcctgatgc gtcagcacaa tcacatcagg ctgagaaggg attgttctga catggctatg
      721 cgttgtcgtt gtccaactaa acgtagcgga aatcacagta tttaagtgac agtgtcacgt
      781 taaataaaaa cccgcgagtg cgggcgagag gaatttgtca gattttcagc ggtaacacgc
      841 tgctcatcca gcctggaatt tcttcggcgg gcagggggcg ggaaaagaaa tatccctgaa
      901 taacgcgaca gtggatcttg cgtagcatct caaattgctc tttggtttcg atgccttcag
      961 ccacgacggt taaattgagg ctttgcccaa tgctggtaat ggcttcgagt aaggcaagga
     1021 tgcgtttttc ggtcagacaa cgatcgacaa aacttttatc aattttgatt tccgttaccg
     1081 gaagactgac taagcgggat aatccggaaa agcctgtacc aaaatcatct accgataaac
     1141 cgatgcccat atcgcgcagg atctgaatgc gcttaaagat ttcggtatcg tgttccatca
     1201 tcatgctttc cgtgatttcc accgtcagct ggtggccgtc aataccccag gcgtgcattg
     1261 catcagatac ctgattaggc agctgattac tgcgaaagtg cagcgcagac aagttaacgg
     1321 ataacgccgg gatatgaata ttctggctac gccattctgc taactgacgg caagcttccg
     1381 cgatgaccca gcgcccaata ttttcgattt caccaatctc ttctgcgaga ggaataaacc
     1441 atgcagggga tcgtaccagc gagcaagggc ttcgatgccg tacagttcac ccgtttctgc
     1501 gaagatttgc ggctgataaa ccagtttaag ttggttattg ctaatcgctt cttttagcgc
     1561 tgcgcctaaa accaaacgct cttttaccat ttcgttcatc gccggactga agaactgcca
     1621 gccgttaccg ccatttttgc gaataaaatc cattgcattg tgagcagtgg agagcaagta
     1681 atcgcggttt ttacccacgt cgtagctgat gccaatgctc aaggtgagcg ggaatggttt
     1741 atcgtcaatc attatcggct tgctgaccac attccgtagc tcatcggcga tctgggtaat
     1801 gttactgaca tcgttttcca ggctcacgag gacaaaagac gtaccttcaa tacgacagag
     1861 atactgatcc ggtttgagtt tttcacgaaa gcgattgacc acttccagca atgcctgatc
     1921 ggcccacgca tagccaaggc tatcaatcac atcctgaata tggtcaacac cgatgagata
     1981 caccaccgga gagacggctt tatcgaccag gtcatcgagg taattgtgca ggttattgcg
     2041 atttggcaga ccggtcattg gatcaaattg gatgagttgt tcaatatgct gacggctttt
     2101 ttcctgttcc agcgccagcg cggccatatg ctggctgata tctgccacgc gttcgataaa
     2161 ggcgctggtt tctgctcctg acgaggtttt aatttgcagg atccccgcag gcgcgccatc
     2221 gcgctgacga atggtcgctg accagctttg cgcattttga acttctgcac cgtgcgaaga
     2281 tgacgcccag tgtattggca tcccgttgcg cagcgcgaac agcgaaacat gcgattcgtt
     2341 gagaacagat tcgatgttac gacaaatgat ttcccccatt tcatgaaatg gtgggctgct
     2401 gcacatggcg gcgagaatat tgccttcaag ctggcggatt tgcctttctt cggtgatatc
     2461 cgagaatgtc attaccaggt tctgcagatg cgcgagcacg tcataaaccg ggctgataga
     2521 ggctttaatc cagatttttt caccggtgcg cgtcaacagc agaaattcgt cctgatcgcg
     2581 ggcggttttc catagcaact gctgtaaacg aatacggtta tcagcaggga attctggaat
     2641 gttcaggagt gtatcgggct gcataccgct ggcttcgtta atgcagtaac caaacatttc
     2701 ggtaaatgcg cgattgcact gcacaatatg gcgttccgga tcgaggacaa tcaccggtcg
     2761 gtcgagatgg tcaacggcaa taatcaattg tcgggtctgt tctttttgcg ccatttctac
     2821 gctggcatcc cgtaccagcg ccaggtaata aactttcccc tcggcgctca ctttcgatag
     2881 cgcaaaacgg gtccagactt tactgccgtc ttttttctcc agctgcagct cccgactcat
     2941 cccctcgacg cgcgctttac caccttcacg gttgtgacga atgtattgag gatgcgcagg
     3001 acgcaaatcc cgcggaatca gcatatcaat gttattgcca atgacttctt cacgtttgta
     3061 cccccagagc ttctctgcgg cggggttgaa aaacatcact tcatcatttt cgttaattaa
     3121 caccgcgccc atcatatttt gctcaagggc ggggaaaaaa atgccatcgg cggcattatc
     3181 cgcatcggtt agcttcatga ttacctctgc atcctggcgc atctaaagac tggctttaca
     3241 gagttcaaca cggtttctac ctcgtctttt ggcgatatac agagcttcat cggctatttg
     3301 aatgaggcgc tcatagtcag gatgaccatt aaacatggcg gcaccgatgg aaagtgagag
     3361 ggcaatatcc tcgccgtttg cggctttaaa ataatgtcga cctttatggt taaaccgcag
     3421 gccaaaatca accaaacaca acgtgcggga tcgactaact gcaccatatt ctcctgaaat
     3481 atgaagatat actgaaaaga aataagcgac ttaggacagt ttcaatctac gctactgttc
     3541 ttcagaagag tatagcccat cgtaattatt tttcggtgac agcgaatatc gtatgttttt
     3601 tcatattcat acatttttat tagggggatt atggctgttt aactaagtgt ggttaattgc
     3661 gtggttaatt tgacgtattt aagcatgatt gctagtggga tagttcaaga gggtaacaag
     3721 ccagtgggta aagcaccggc ttgttacaaa gtaagaatgg gnnnnnnnnn ngagtttaac
     3781 tgccccaacg actttgcaga tagctgaccg cttgttgcgt ctgcggttta ttcagatagt
     3841 cctcacggaa caagattgtg ccgctaattt ccggcacagc atcgttaaga tcgagctgct
     3901 ttttcagttc cggtacgccg ccgttaatca tccagtctgg ctctatcttt gaaggttcac
     3961 ccactttata gaaggcgata ccgatataca ggcgggtcct ggtcggttta acgacatccg
     4021 cccaccattt tgccaacacg tcataacgcg cggcactccg tgagaatggc cagtaaattt
     4081 ggggagcaat gtaatccagc aacccttgtt ccacccatcg acgggtatca gcgtaggatt
     4141 cgtcataggc tgccgcgcct cgggtatcgg aaccgagcgg atcgtgtgat cggttacgcc
     4201 acacgcctgc cgggctaacg ccaaattcga cttccggctt aatgctttta atggtgtgcg
     4261 ataccttcgc aattaactgc tgagtattgt tgcgccgcca gtctgctttt gacgcaaatg
     4321 cgccgccgta tttgcggtac gtttcgttat catttagccg tgaacctggt gactcagtat
     4381 agaaatagtc atcaaactgc acgccatcta ctggatagcg ggaaaccact tccgcgacta
     4441 ttgatgtgat ccagtcctga acctcaggga tgcccgggtc gaggacaaag cgatcgccgg
     4501 acgttctgat ccagtcgcgg tgttgcacat agacgctcgc cggttgttga gacagagtgc
     4561 tattcagttc cctgatagta ccgggcttcg tattaaccga tacgcgatag gggttaaacc
     4621 aggcgtgtac tttcatccca cgcttgtggg cttcgtcgag cataaattgc agcggatcgt
     4681 aacccggatt ttcaccaatc ttaccggtca taagatcgga ccacggcaaa attttcgatg
     4741 gccacagggc agtaccatcc ggcttgacct ggaaaaagac cgtgtttatg ccgagacgtt
     4801 gcagatgatc cagtttgtcg atcatcgcct gttgttgtac acgtgcccgg ctggtggggt
     4861 tactaatgtt aaccgaggaa accggtggcc agtcgaggcg agacaccgtg gccagccaga
     4921 tgccacgcat cggttgtgac gattgttgcg tcatggctgg tggctttgaa cccgctggtg
     4981 gtgtcaccat ggactctgga ggcgtgcttt tacaactaca cagtaaaagt gccagtgcaa
     5041 ccagtatcgc tggtcttcta atcgctaatt tctcgtttcg ggagcagata tccatatgtg
     5101 ctggtttccg gtaaacagat gtgcgctcat tctcgtactt atcccggtta agtcaatacg
     5161 acaacaagca cgaaaaaggg agcgatgaat tatcgctccc ttgtcttata accattcaga
     5221 catggttagt gtttcttgtc attcatcaca atatagtgtg gtgaacgtgc acgcgggtgc
     5281 aggaagaagt gaccttttgg tgcgttctga ctgttgattg gctccagagt gacgccggta
     5341 ttcgctttgc ttttacgatc atgaacagca tagagaataa agggcagggc aagtaccacc
     5401 aggaaactaa cgaccagtaa ttcaacatac atatcggtag agtcaccctg gatgttatcc
     5461 ggcggcagga aggaaacaat aaacgccata attgaagtca gcagaccgac aatagccacg
     5521 accagtttca cccctttacc accagggata ttaaatgtgc gttttaagtc aggatgttta
     5581 agaaccaaca caatgtagcc aataaacagc atgaaataag cacacagata aatcaccacc
     5641 gtcagcgcca gtgcgatcag g
//
LOCUS       DAWDRC010000219         5656 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6428_length_5656_cov_2.065524, whole genome shotgun sequence.
ACCESSION   DAWDRC010000219 DAWDRC010000000
VERSION     DAWDRC010000219.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6428_length_5656_cov_2.065524"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1904..1913
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4729..4738
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1511 a         1506 c         1245 g         1374 t
ORIGIN      
        1 tcttattcct tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg
       61 taaacgaagg gggaccattg gtgaatgatc tgttttgcac aataccgggc ctgcatcacg
      121 ctggcccgcg gtttcaggga atgagcaaca ctttgcccgg catggcatta cgagcgatgt
      181 cacgcaccgc ctgggcgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc
      241 ttaacttacg ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc
      301 tggagtagtt catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg
      361 ttgcagatgt taaatgcagc tcctgatgca acgtgcccac cagcgccagt tgggcatgag
      421 gaccggcaat ctctaccgcc agttcgacgg tttgcgggac gccagccgtc tcgaggataa
      481 gctgattaaa gcgcagatcg cgtaaaacgc cctgtatttg cggcgcgctc atttcacggc
      541 tgttaaatgt ttgcatcgca ccgaaagatt ttgccagtgc cagtttttct gagctaatgt
      601 cgatagccgt cacactcttt gctcccaacg cgacagcgca ctgaattgcc agcaggccaa
      661 tggttccggc accaataata ataacgtttt tattctcaca tccttgcgct aaatggaacg
      721 catgcaggcc aacggtaatc ggttcaataa aagcgccatc ctcaataggc atatccgcag
      781 gtagtgcaaa gacattgttt cgtttaacga caatatattc ggcaaatcca ccatcacgcc
      841 gtgagccaat aaaatcatat tttgcgcact gggaataaaa ccctttcaga cactctggac
      901 aagtaaaaca gggtaataac ggcacacagg caaccgcatc gccggggtgt aaatcatcaa
      961 caccggatcc caccgcatca atatagccac taaattcatg gcctaacgtt attggataat
     1021 aatgtgcacc atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa
     1081 tttttacccg cacctcatcc tgatgtttaa tttcaggaat gacgctttct gctacgcgca
     1141 cgataccatc agtatcattc accactgatt tcataaaaac tcctgattgt taagggggat
     1201 aacctccccc tcaggtaaaa attattctgc gagaacgact ttctcttgtt tgataaagcc
     1261 gcgcgcccta cgccaggtca tgaaaatacc ggtgagataa atggcgccga taataatgaa
     1321 accgggaata ttttgcgggg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc
     1381 ctgatccatt gaagccacca tacccccggc ttttaacgcg cccgcattag ccgccagttg
     1441 ggtgtgcaaa ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc
     1501 cgagattaag gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacaaagaa
     1561 gccgatggtg gcaaggtcgc caaacggcag cacctgatta cccggcacac aaacagcaat
     1621 taaaatggtg agtggaataa aaatcaggct tgccgatacc accgccgtat gtcccagcag
     1681 caacgccgga tcaaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact
     1741 acgggcctgc ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat
     1801 cagcagcata actgccgccg tttttaccgc cagctgcaat gcacctttga catcgtaacc
     1861 cgcgaggatg ccgataatca gccccatcac aaaacccacg gtgnnnnnnn nnnaaagggc
     1921 caaatttgcg ctgaatatca tcggcgctaa atttaattcg gttaacgcct gggattttct
     1981 cgatgatagc atcgaccagc accgcaatcg gccccatata cgccgacgta ccgtgcggaa
     2041 tagcaatgcc ttccagctca aagaaatttc gggtatcgcg ggcaaaccag tcgccgagct
     2101 tataaacaaa cgccgcgtga attaccacac ccgccatccc tatcatccat gaaccggttg
     2161 ccagatgcag caacgcgccg gtgaaggtca tatgccagat attccagata tcaacattta
     2221 ccacccgcgt catacgggtc agtaacatcg ccacgttaac cagaatcgca atcggaatcg
     2281 ccaccagcgc aatttgcgac gcccaggtca tgggtgaaga gcccggccag ccgacatcga
     2341 ccacatgcag attcaggtcg aaattttccg ccatcgcttt cgccgccggg ccaatggaat
     2401 ccagcattaa gccaatcaca aggccaatgc caacaaagcc aatcccgata tgcagacctg
     2461 ctttaaagca atcgcctgcc ttcatgccta atattttaga aaacataatg atgacaatcg
     2521 gcagcatcac cgtagggccg aggtcgagaa tataacgcat gacttctgaa aacataggtc
     2581 acccctgtaa gatagtcaga attttatttt gtaatgcttc gataccgaca ccagaaacaa
     2641 aaggcatgcc gtgaactaac ggaatatcgc caaaactacg atccaccctg gcagtggtgc
     2701 atatcaaatg cacgccatcc atataggttt ctatttcatt aacccgacac tggattaatt
     2761 caacaggaat attatgactc tgacacaact ctttaatttc ttccgccgcc atcgtagagg
     2821 tcgcaaccgc gcctccgcaa gcgacaataa ttttgcgttt cataaatagt cctttttatt
     2881 gttacagagg gatttattcg tctgaatcta aaacatactt tgtgaagtat tcctttaact
     2941 gggtttcagg aagagtgatt agtgtctcga cgatatcagg ctgttgtaac ttgccaaata
     3001 aacagcgtaa aagtttcaat tgctgctgcg gattttccac aatcaacgca ataaccaacg
     3061 ataccgccac gtcgttatca tcatccgctt gctgaaaatg aactttattt gttggcctta
     3121 acagataaat ggctgacgac ttagcatgaa tcgcctcaca atgcggtatt gcaatggcgt
     3181 gctgctcaag cattatcccg gtagggaatt ctgcttctct ggcaattaat gcctgtggcc
     3241 aggtatcatg aaccacacct ttcgcgagca tctcattacc gatatgggtt aaaacttcgc
     3301 tacgatcgac aaaagaaatt ccgctacgaa caaacaggtt agtcatatac cgtccttatt
     3361 ccgcacagcc gtagcgatag gcgcgcaaaa catcataaat tttatccata ataagctgat
     3421 gcggtattgc tgataattcc ccggactgaa tgcgttcaaa ttgtttcgga agatactgac
     3481 taatcatgcc cagtgggatg tccacgcctt ccagattcac catcatcgtt tcgacgctat
     3541 ttttaatccg gctatgcggc cagtaataac gaatacgatc cgacaggctg taacgaatat
     3601 ccagtaatga atcgttaaaa cccgtgcgat aatatttttt ccagtattgc ggttcgtcga
     3661 gcatcacttc ttcaatcacc gccagacaac ggctacgatt ttctggagcg atgagttcct
     3721 gctcaatttg tgccagcgca aatatcgcct cacgtaaagc aaaggttaat gccggaccga
     3781 ctttcaaaat tgcaaaatga tcgcgaacta attcccgata ggccgtctga gtctggtaat
     3841 cggtggaatg cgcttcataa accattttgg ttttttctat ccattgcgcc aacgcctgag
     3901 cttcttgcgc ctgataatga ataatattgc tatggtcaaa ttccacaccc ggctgcacca
     3961 cgatggcaat cacgcgtgtt aacgcctctg ccagaccgcg ggcaataaac gctttttgat
     4021 gcgtgcgtaa agtattggcg gcatcttcaa cctgggtgat gtgtactgac tgaatggcgc
     4081 tggcctcacc gcccggaacc ggaacttcag tgccaatgac atagttcagt tgctcacgct
     4141 ggcaatcagt cgccacactt tccgccgcca gacaaagcac agcggcacgt tcagcaaccg
     4201 tttctggcgc taacggtatg gaatcgtcag cgcaggacat tgacgcatca agatgaattt
     4261 tactgaagcc ggcacgaaca tatgccttta ccagctcgac ggatttttcc atcgccgcat
     4321 ccgcattttc ttgctgccag cagtttggtc ccagatggtc gccgccgaga ataatacgct
     4381 cacgagcaaa tccgacttta tcggcaatcg caaaaacaaa ttcgcgaaag tctgccggtg
     4441 tcattccggt ataaccgcca aattgattga cctggtttga cgttgcttca atcagcactt
     4501 tacgcgtgct gttgcgatca aatgccagcg ccgcttcgat aaccaacgga tgtgcagaac
     4561 agactgaaca tatgccaata tgttcaccag ctttatgccg ggcaattaac gttttcatgt
     4621 tttttccttg ttaaatggca ggtgcgttat gccctgccct cgcagccaca atcggcaatc
     4681 actttgctca ccacatcgcg cattgcagat ttagccgact gcaaataann nnnnnnnnaa
     4741 taatcccggg gatcggtcgc ttcggggtac tctgtcaggt aattttttaa cgcctgcgag
     4801 aaggcatttt tcagctccgt tgcaacgttg attttgcata tccccagttt gatggtttgc
     4861 tgaatatcct ttgtcgataa tcctgacgcg ccatgcagca ccagcggtaa gttcacccac
     4921 tggcgaatgt tctccagtcg agaaaaatca agcgccggtg cgctggcata catcccatga
     4981 gccgtgccga tggcgaccgc cagggaatca atcccggttg cctcggcaaa ttcacgcgcc
     5041 tgaacggggt tggtgtacaa cgcatcggct tcattgactt gcacatcatc ttcctggccg
     5101 ccaagttgcc ccagctccgc ttcgacgctg acatcaaagc gatggcaaaa atccaccacc
     5161 tctttgacca ctgaaatatt ttgcgcaaaa ggcaaatgcg aggcgtcaat catgactgag
     5221 cgcacgccag aacgaacctt ctgagcgata tcgtcaaatt tcgtgtgatg gtcgagatga
     5281 atcgccagtg gatggtgata ttgcttcgcc atcgcgctga ccagcgccat cagattttct
     5341 gtaccggcat gagtaaatgt gccgggcgtt ccggcgatga tgaccggcgc atgcatgctg
     5401 gcagcggttt ccaccaccac ttgcatcgtt tcgagattgt gaatattgaa tgccggaacc
     5461 gcataaccgc cgcgccgcgc gttgttcagc atctgctttg tcgataccac gtacattttc
     5521 acatcctgtc atttattttc gatttcaaaa cataatgaaa ttatttgttt ttaaatgtcg
     5581 cgataacgat cacaaaaacg acaatatgaa aatcaatcca ggaatgaaag gcaaaaaaaa
     5641 cggcctcccg ggggaa
//
LOCUS       DAWDRC010000220         5649 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6437_length_5649_cov_2.398999, whole genome shotgun sequence.
ACCESSION   DAWDRC010000220 DAWDRC010000000
VERSION     DAWDRC010000220.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6437_length_5649_cov_2.398999"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    255..264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1051..1060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3640..3649
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4559..4568
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1350 a         1437 c         1464 g         1358 t
ORIGIN      
        1 ttgccatttt tgttatctcc cttaatcaaa cttcattcgc gtggctttca ggttcactat
       61 cgccagcgcg cccaccagca ggaagatcag cgtggcaatt gctgctgcca gaccgaagtc
      121 ctgacccccg ccgccttcaa aagcgatacg gtaggtgtag ttaacaagca ggtcggtata
      181 accggctggc gtggtcgtgc caagacgatc cgggccgccg ttggttaaca gttgaatcag
      241 cacgaagttg ttaannnnnn nnnnttgtta aagttaaagg cgaagctggc gatcatcagc
      301 ggcgtcagcg gtttaatcag cagcggcagc gtaatcttaa agaagttctg gaatggacct
      361 gcgccatcca ttgctgaggc ttcatacaaa tcgtccggaa tcgccttcag caagcccatg
      421 cagaggatca tcatgtacgg ataacccagc caggtgttga cgataatcag catcgtgcgg
      481 gcggtggtcg gatcgctgaa ccaggcgggc ttcacgccaa acagcgcgct caacatcatg
      541 ttgatttcac caaaactctg gttaaacaac cctttgaaaa tcaagattga aatgaacgat
      601 ggcaccgcgt agggcagaat aagcaggacg cgatagaccg ctttgccgcg caacgcttcc
      661 cactgcacca gacacgccag aaccatgccg accgccaccg ttaaaaagac agtgatcagc
      721 gagaacacca cggtccagac gaaaatggcg aggaacggtt tctgaatgcc ttcgtcggta
      781 aagacgcggg taaagttttt ccagccggtg gtcacggtgt aaccggggct tagcttttca
      841 tccccccagt tgccatcggc ggtaatggac tggtaaaagc caatttggtt attcggacga
      901 tatttcacgc cgctctgatt attggtcaac gtgccgtcac cgtcgagtgt gtagagcggt
      961 tgcgtgccag aaaactggcg cagggagctc atcatcactt tgttgccatc cggcagaatg
     1021 gcggtaatgt cactcagtgc ctgacgattc nnnnnnnnnn ctggttagtg ctgctgtagt
     1081 tggtgaaggc aatggcgatg gtgcagacca gagggaagag gacgaataat cccattccag
     1141 ccattcccgg gtaaacatag cgccaggcgt aggctttacg attggcgaaa atatacagcc
     1201 ccgctgagct caatatcagc gtggtaatgg cgaacaggta ttccccttgt gcgtacatta
     1261 aaacaacaag gtaacccacc agcaggccga gcagacctaa cactgaccat ttcagcgcgt
     1321 cgctttgcca ccaatgtttc tttttaatga catccatggg gttcttcctc attccaggac
     1381 ggataaggct ttcatacctt atccgacaac aactgcctga tgcgacgctg acgcgtctta
     1441 tcaggcctac atacgtttca atttcgtagg ccggataagg cgtttacgcc gcatccggca
     1501 tttcacagca ttacttggtg atacgagtct gtgcatcttt cagcgcagca tccacagtct
     1561 gacgaccgct ggcggcgttg atcaccgcag tacgcacggc ataccagaaa gcggacatct
     1621 gcgggatgtt cggcataatt tcacctttct gagcgttatc catggtggcg gcaatacgtg
     1681 gatctttcgc aagttcatcc tgataggact tcagcgctac tgcacccagc ggtttgtctt
     1741 tattgaccgc ttccagacct tcgtcagtca gcagatagtt ttcgaggaac tctttcgcca
     1801 gctctttgtt cggactggcg gcgttaatac ctgcgctcag cacgccaacg aacggtttag
     1861 atggttgacc tttgaaggtt ggcagtaccg ttacaccata attcacttta ctggtgtcga
     1921 tgttggacca tgcccacggg ccgttgatag tcatcgctgt ttcgccttta ttaaaggcag
     1981 cttctgcgat ggagtaatcg gtgtctgcat tcatgtgttt gtttttaatc agatcaacca
     2041 ggaaggtcag acccgctttc gcgccagcgt tatccacgcc tacgtcttta atgtcgtact
     2101 tgccgttttc atacttgaac gcataacccc cgtcagccgc aattagcggc caggtgaagt
     2161 atggttcttg caggttgaac atcagcgcgc tcttaccttt cgctttcagt tctttatcca
     2221 gcgccgggat ctcttcccag gtttttggcg ggttcggtag cagatctttg ttataaatca
     2281 gtgataacgc ttcgacagcg atcgggtaag caatcagctt gccgttgtaa cgtacggcat
     2341 cccaggtaaa cggatacagc ttgtcctgga acgctttgtc cggggtgatt tcagccaaca
     2401 ggccagattg agcgtaacca ccaaagcggt cgtgcgccca gaagataatg tcagggccat
     2461 cgccagttgc cgcaacctga gggaatttct cttccagttt atccggatgc tcaacggtga
     2521 ctttaattcc ggtatctttc tcgaatttct taccgacttc agcgagaccg ttatagcctt
     2581 tatcgccgtt aatccagatt accagtttac cttcttcgat tttggcgaga gccgaggcgg
     2641 aaaacatcat cgtcgttaat gcggataatg cgaggatgcg tgcacctgtt tttattttca
     2701 taatctatgg tccttgttgg tgaagtgctc gtgaaaacac ctaaacggac tctagtttct
     2761 ttatacggca acctctttcc atcctccttg cccctacgcc ccatcgttgc tttgtgtgat
     2821 ctctgttaca gaattggcgg taatgtggag atgcgcacat aaaatcgcca tgatttttgc
     2881 aagcaacatc acgaaattcc ttacatgacc tcggtttagt tcacagaagc cgcgttctca
     2941 tcctcccgcc tcctccccca taaaaaagcc agggggtgga ggatttaagc catctcctga
     3001 tgacgcatag tcagcccatc atgaatgttg ctgtcgatga caggttgtta caaagggaga
     3061 agggcatggc gagcgtacag ctgcaaaatg taacgaaagc ctggggcgag gtcgtggtat
     3121 cgaaagatat caatctcgat atccatgaag gtgaattcgt ggtgtttgtc ggaccgtctg
     3181 gctgcggtaa atcgacttta ctgcgcatga ttgccgggct tgagacgatc accagcggcg
     3241 acctgttcat cggtgagaaa cggatgaatg acactccgcc tgcagaacgc ggcgttggta
     3301 tggtgtttca gtcttacgcg ctctatcccc acctgtcagt agcagaaaac atgtcatttg
     3361 gcctgaaact ggctggcgcg aaaaaagaag tgattaacca gcgagttaac caggttgcgg
     3421 aagtgctgca actggcgcat ttgctggatc gcaaaccgaa agcgctctcc ggtggtcagc
     3481 gtcagcgtgt ggcgattggc cgtacgctgg tggccgagcc aagcgtattt ttgctcgatg
     3541 aaccgctctc caacctcgat gctgcactgc gtgtgcaaat gcgtatcgaa atctcccgtc
     3601 tgcataaacg cctgggccgc acaatgattt acgtcacccn nnnnnnnnna cccacgatca
     3661 ggtcgaagcg atgacgctgg ccgacaaaat cgtggtgctg gacgctggtc gcgtggcgca
     3721 ggttgggaaa ccgctggagc tgtatcacta tccggcagac cgttttgtcg ccggatttat
     3781 cggttcgcca aagatgaact tcctgccagt aaaagtgacc tccaccgcaa tcgatcaagt
     3841 gcaggtggag ctgccgatgc caaatcgtca gcaagtctgg ctacctgttg aaagccgtga
     3901 tgtccaggtg ggagccaata tgtcgctggg tattcgcccg gaacatctac tgccgagtga
     3961 tatcgctgac gtcatccttg agggtgaagt tcaggtcgtc gagcaactcg gcaacgaaac
     4021 ccaaatccat atccagatcc cttccattcg tcaaaacctg gtgtaccgcc agaacgacgt
     4081 ggtgttggta gaagaaggtg ccacattcgc tatcggcctg ccgccagagc gttgccatct
     4141 gttccgtgag gatggcactg catgtcgtcg actgcataag gagccgggcg tttaagcacc
     4201 ccacaaaaca cacaaagcct gtcacaggtg atgtgaaaaa agaaaagcaa tgactcagga
     4261 gatagaatga tgattactct gcgcaaactt cctctggcgg ttgccgtcgc agcgggcgta
     4321 atgtctgctc aggcaatggc tgttgatttc cacggctatg cacgttccgg tattggctgg
     4381 acaggtagcg gcggcgaaca acagtgtttc cagactaccg gtgctcaaag taaataccgt
     4441 cttggcaacg aatgtgaaac ttatgctgaa ttaaaattgg gtcaggaagt gtggaaagag
     4501 ggcgataaga gcttctattt cgacactaac gtggcctatt ccgtcgcgca acagaatgnn
     4561 nnnnnnnnac agaatgactg ggaagctacc gatccggcct tccgtgaagc aaacgtgcag
     4621 ggtaaaaacc tgatcgaatg gctgccaggc tccaccatct gggcaggtaa gcgcttctac
     4681 caacgtcatg acgttcatat gatcgacttc tactactggg atatttctgg tcctggtgcc
     4741 ggtctggaaa acatcgatgt tggcttcggt aaactctctc tggcagcaac ccgctcctct
     4801 gaagcgggtg gttcttcctc tttcgccagc aacaatattt atgactatac caacgaaacc
     4861 gcgaacgacg ttttcgatgt gcgtttagcg cagatggaaa tcaacccggg cggcacatta
     4921 gaactgggtg tcgactacgg tcgtgccaac ctgcgtgata actatcgtct ggttgatggc
     4981 gcatcgaaag acggctggtt attcactgct gaacatactc agagtgtcct gaagggtttt
     5041 aacaagtttg ttgttcagta cgctactgac tcgatgacct cgcagggtaa aggtctgtcg
     5101 cagggttctg gcgtcgcgtt tgataacgaa aaatttgcct acaatatcaa taacaacggt
     5161 cacatgctgc gtatcctcga ccacggtgcg atctccatgg gcgacaactg ggacatgatg
     5221 tacgtgggta tgtaccagga tatcaactgg gataacgaca acggcaccaa gtggtggacc
     5281 gtcggtattc gcccgatgta caagtggacg ccaatcatga gcaccgtgat ggaaatcggc
     5341 tacgacaacg tcgaatccca gcgcaccggc gacaagaaca atcagtacaa aattaccctt
     5401 gcacaacaat ggcaggctgg cgacagcatc tggtcacgcc cggctattcg tgtcttcgca
     5461 acctacgcca agtgggatga gaaatggggt tatgactaca ccggtagctc ttcaaccaac
     5521 ccatactacg gcaaggctgt gtcggctgat ttcaacggcg gtagcttcgg tcgtggcgac
     5581 agcgacgagt ggaccttcgg tgctcagatg gaaatctggt ggtaatagca aaaaactggg
     5641 ccggataag
//
LOCUS       DAWDRC010000221         5621 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6473_length_5621_cov_1.967481, whole genome shotgun sequence.
ACCESSION   DAWDRC010000221 DAWDRC010000000
VERSION     DAWDRC010000221.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6473_length_5621_cov_1.967481"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    298..307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    632..641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2895..2904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3749..3758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4309..4318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1325 a         1416 c         1336 g         1494 t
ORIGIN      
        1 ttcgtcgctg gctggttgct ggtagcggaa tttattttca ctctggcgat ggccctgaaa
       61 tgctacccgc tgctccccgg tggtctgttg gctatcgaag cggtattcat cggcatgacc
      121 agcgcggaac atgttcgtga agaagtggcg gcaaatcttg aagtcttgct gttactgatg
      181 tttatggtgg cgggtatcta ttttatgaaa cagctgttgt tgttcatatt tacccgtttg
      241 ctgttaagta ttcgctccaa aatgctgctg tcgctctctt tttgcgttgc ggcggcgnnn
      301 nnnnnnncct ctccgcgttc ctcgatgcct taaccgtcgt ggcggtggtg atcagcgttg
      361 cagtcggttt ttatggtatt tatcaccgcg tagcctcttc ccgtactgaa gataccgacc
      421 tgcaagacga tagtcatatc gacaagcatt acaaagtggt tctggaacag ttccgtggct
      481 ttctgcgtag tctgatgatg catgccggtg tcggcaccgc attaggcggc gtgatgacca
      541 tggtgggcga accacaaaac ctgatcatcg ctaaagcggc tggctggcat tttggcgatt
      601 tcttcctgcg catgtcgccg gtgaccgtcc cnnnnnnnnn nccggttctg atttgtggcc
      661 tgttaacctg cctgctggta gagaagctgc gttggtttgg ctacggtgaa acgctgccgg
      721 agaaagtccg cgaagtgctg caacagtttg acgatcaaag ccgtctccag cgtacacgtc
      781 aggacaaaat tcgtctgatt gtgcaggcga ttatcggcgt ctggctggtc accgcgctgg
      841 cactacatct ggcagaagtc ggtttgattg gtttgtcggt cattattctg gcgacatcat
      901 tgaccggtgt taccgatgaa catgctatcg gtaaagcctt caccgaatct ctgccattca
      961 ccgcactgtt gacggtgttt ttctcagtag tcgcggtgat tatcgaccaa caactgtttt
     1021 cgccgattat tcagtttgtg ttgcaggcat ctgaacatgc tcagctgtcg ttgttctata
     1081 ttttcaacgg tctgctgtca tccatttcgg ataacgtctt cgtggggacg atttatatca
     1141 acgaagcgaa agcggcaatg aaaagtggcg ctatcacgtt gaagcaatat gagctgctgg
     1201 cggtcgccat taataccggt accaatctgc cctccgtcgc tacgccgaac ggtcaggctg
     1261 cgttcctgtt cctgctgacc tctgcactcg cgccattgat tcgtctctct tatggccgca
     1321 tggtgtggat ggccctgcct tacaccctcg tcctgacact cgtcgggctg ctctgcgtcg
     1381 agtttacgct tgctcctgta accgaatggt ttatgcaaat gggctggata gcaacgcttt
     1441 gataacaact taccgggcat ataaatgccc ggtttgcctt ttcgcccgat aattgtccaa
     1501 ttgcgtccta tttttgctgc ctcctggtgg cgcagcgaat gaattggttt aaactgcgca
     1561 ctctatgcat attgcaggga aatgattatg ttgcgatttt tgaaccaatg ttcacaaggc
     1621 cggggtgcat ggctgttgat ggcgtttact gctctggcac tggaactgac ggcgctgtgg
     1681 ttccagcatg tgatgttact gaaaccttgc gtgctctgta tttatgaacg ctgcgcgtta
     1741 ttcggcgttc tgggtgctgc gctgattggc gcgatcgccc cgaaaactcc gctgcgttat
     1801 gtagcgatgg ttatctggtt gtatagtgcg ttccgcggtg tgcagttaac ttacgagcac
     1861 accatgcttc agctctatcc ttcgccgttt gcgacctgtg attttatggc tcgtttcccg
     1921 gaatggctgc cgctggataa atgggtgccg caagtgtttg tcgcctctgg cgattgcgcc
     1981 gagcgtcagt gggaattttt aggtctggaa atgccgcagt ggctgctcgg tatttttatc
     2041 gcttacctga ttgtcgcggt gctggtggtg atttcccagc cgtttaaagc gaaaaaacgt
     2101 gacctgttcg gtcgctaatc cattcggcgc tcctgcggga gcgctttttc ctgccgctat
     2161 gtttatttga cccgcagtaa atcagaactt cgcgttgtat aacatggtga aagcatggca
     2221 cgcttcatct gccattgttg ctggatcccc tgcccggcaa aatagagtgt tcctctgccc
     2281 tctttagcat tcagcgtatc cattaccgcc atcaattgct cactcccggg gcgcggtgcg
     2341 ttgtcatcga ataaattgag ctgcgcgact ccctgactga agaaatcccc cagcatcacg
     2401 cccgcttttt ggtagcgatg gcccgcttgc cagatggcat ccagagatcg cgtagcggcg
     2461 ttaatgatat ccctgctgtc ctgagtgggc gtcagcagtt ttaccgatgc gctattgccg
     2521 taataaggtt cattgaacgc aaatggtgac gtcttaataa acgtggagat aaaccgacaa
     2581 tattgatgct cgctgcgaag tttttccgcc gcccgggcag cgtaactaca aatggcctgc
     2641 cgcatcgacg gataatccgt gatgcgttca ccaaacgagc gggaacagat aatttcctgc
     2701 ttcgtcggtg caaactcttc cagttgcaaa cagggttcac cgcgcagttc acgcaccgtt
     2761 ctttcgagca cgacattaaa atgtttacgg ataaaccgga tatctgaatc cgccaaatcg
     2821 agaacggttt tgatccccat cgcgtccagt tttttgctga tccgccgtcc aatcccccag
     2881 acttcatcca cgggnnnnnn nnnncagaca ttaatttacg ctggcgttcc agatttgata
     2941 aatccaccac cccgcccgtc tgccgctgcc atttttttgc cgcatgattg gcaagcttag
     3001 ccagcgtttt ggtctgggcg atccccacac caacagtaag atgggtacgt tgtagcaccg
     3061 ttgcgcaaat ttctctgcca aaatcagtca gatcgcgaca attatgcaca cctgtcagat
     3121 cgcagaatgc ctcatcaata ctgtaaatct cgacgcgggg cgatagttct tccagcgtcg
     3181 acatcacccg attactcatg tctgcgtaaa gctcataatt gctgctaaag caaaccacgc
     3241 cacagcgacg aaacagatct ttttgtttga accagggatc gcccatttta acgcccagcg
     3301 cctttgcctc agcgtttcgg gcgataacgc aaccgtcatt attcgatagc acaaccaccg
     3361 gtttacccca taaatcaggg cgaaacaccg tctcacagct ggcataaaac gcgtttacat
     3421 cacagagggc aaacatcagc gcatcgcctt aacgacgtgg atcaccacac caaagacatc
     3481 cagcgtatct tcgctactga tggtaatggg cgagtacgca ctgttcatag gagtaagctg
     3541 taccgtcggg cgtagttgca attttttcac cgtaaactca ccgtcaacag cagcgatgac
     3601 aatatcacca tggctggcgg taatagcgct atcgacaatc agtaaatcac cgtcactaat
     3661 cccaccatca atcatagaat caccacttgc tttgacgaaa taagtcgcgc tgggatgctg
     3721 gatcaacagt tgattcagat cgatgcgcnn nnnnnnnnaa cgtaatctgc ggccggtgaa
     3781 ggaaagccac actgaacaag atcgctaaat agcggaaaag tcacaatttc gcggagatcc
     3841 gcaggcttga taaacaacat aataatctgc ctgaagttat actgttttta tatacagtag
     3901 tctgttcttg tcagcagatc aatactgatt caggctatca atatttgtcg ctgcatagac
     3961 tgctgatttt tcgttctctt atcctgtgct cacgcggcct tctggcgaca gcgtccatcc
     4021 agcagaaagg aaaaatatca cctggtttaa aatagaatcg aagcattcgc ccagaaaggc
     4081 attctcatta aggtactgga tgacggcatc aagaaactgt gttctattgc tgcgggcgta
     4141 gttgaaggga aactgattcc agaattgaag aacaagttaa aatctgtgca gagtttcttt
     4201 accaccctgt ctaacacggt taaacaagcg aataaagata tcgatgccgc caaattgaaa
     4261 ttaaccaccg aaatagccgc catcggggag ataaaaacgg aaactgaann nnnnnnnnga
     4321 ttatgatgat ttaatgcttt ctttactaaa agaagcggcc aaaaaaataa ttaacacctg
     4381 taatgagtat cagaaaagac acggtaagaa gacactcttt gaggtatctg aagtctgata
     4441 agcgattatt ctctctatgt actcaaggta taaagtttat cacatttaaa aatacagacg
     4501 ttttttaaag attgagagtt acctgttgat aataaggtat cgagtaactc tcattatgac
     4561 agagcgcttt caaaaacagg gctacgaaat ggcgagcatc cagacgtgaa ttaagaaagc
     4621 ccccatcaac atctattaaa tttatcactg cgcccagtca ggtttattta agatatgatc
     4681 ctgccagtca atcacttctg attcttttac tgcgatatga cgcacagaga tacgttcacc
     4741 atgcattgcc gctttcgaac cagtaagtag cggatgccac gcaggtaaac ctttaccttc
     4801 cgccagcaaa cgataagcgc aggtcattgg cagccattcg aatgttggca gattttcacg
     4861 ggttaattta atgcagtcgg gttcaaactc gaaacgacgt tcgtagttcc gacattgaca
     4921 ggttttaata ttgagctggc gacaggcgac gttagtgaag tagatttcgt cggtgtcttc
     4981 atccatcagt ttatgcaggc aacactgacc gcaaccatca cacaacgact cccattccgc
     5041 atcgctcatt tcgtccaggg ttttactttg ccagaaaggt acatcgctca tcaggtgttc
     5101 cgccattacg ttaaaacgca ccttataacc agtctggcac agcgatgcaa gttttgccgc
     5161 cgctttcagg cggcaaaaag tattacaaaa cgcgagttgt caaagaatgc ccatcgaaag
     5221 tgactgtcag ctcatcaccg ctttgcaacg ggccgacacc atcaggcgtg cctgtcagca
     5281 caacgtcacc ggccttgagc gtaaaaaact tgctcatata agcgatcagc ggaacgattt
     5341 tatggatcat gtccgcagtc gtaccttgct ggcgttgctc gccgtttacg ctcaggccca
     5401 gcgttgtatt ttgcgggtcg ccggtaaact ccgccgcggg aataaacccg gaaagcggac
     5461 aagagttatc aaacgctttg gccttttccc acggctgccc ggctttcttc atttttccct
     5521 gaacatcacg caatgtcaga tcgagcgcca cgccgtaacc ggcaatggct ttgcggacat
     5581 gctcttccgt agcctgacgc agcgtcgcgc caatcaaccc c
//
LOCUS       DAWDRC010000222         5610 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6487_length_5610_cov_2.071107, whole genome shotgun sequence.
ACCESSION   DAWDRC010000222 DAWDRC010000000
VERSION     DAWDRC010000222.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6487_length_5610_cov_2.071107"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1917..1926
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4245..4254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1298 a         1415 c         1537 g         1340 t
ORIGIN      
        1 cggcagggct tcgcacctgt attgaagcgt tatgcgatga tgatattgcc tggcaaacgg
       61 cacaaactgg tgataaccag ccctggcagg ttaatgatgt tttaactgca gaactgaatg
      121 cgaggacgct acaacgattg cccggcgata actggcgtgt caccagctac tccggtttgc
      181 agcagcgtgg tcacggtatc gctcaggatc tgatgccacg gctggatgtt gatgccgcag
      241 gcgtggtcag cgtcgttgaa gaaccgacgt taacaccgca tcagttcccg cgcggtgcgt
      301 caccagggac attcttgcac agtttgtttg aagacctcga ttttacccag ccggttgacc
      361 cgaactgggt acaggaaaaa ctggaactcg gcggctttga atcgcagtgg gaaccagtgt
      421 tgaccgagtg gatcacggct gtcctccagg cacctctcaa tgaaacgggt gttagcctga
      481 atcagctttc cgatcgcgat aaacaggtgg agatggagtt ttacctgccg attagtgaac
      541 cgcttatcgc cagccagctt gatgcactaa ttcgccagtt tgacccgcta tccgctggct
      601 gcccgccgct ggagttcatg caggtacgcg gcatgttaaa aggctttatc gacctggtat
      661 tccgccacga agggcgttat tacctgctcg actataaatc caactggctg ggtgaagaca
      721 gttcggctta cacccaacag gctatggcag cggcaatgca ggcgcaccgc tatgatctgc
      781 aatatcagct ttataccctg gcgctgcacc gttatctgcg ccatcgcatt gctgattacg
      841 actatgagcg ccactttggc ggcgttattt atctgttcct gcgtggtgtt gataaagaac
      901 atccgcaaca ggggatttac acaacccgac ccaatgccgg gttgattgcc ctgatggatg
      961 agatgtttgc cggtatgacc ctggaggagg cgtaatgaaa ttgcaaaagc aattactgga
     1021 agctgtggag cacaaacagc tacgcccgct ggacgtgcag tttgccctga ccgtggcggg
     1081 agatgaacat cctgccgtca ccctcgcggc ggcactatta agtcatgatg ccggagaggg
     1141 acacgtttgt ttgccgcttt cacgactgga aaataacgag gcatcgcatc cgctgttggc
     1201 gacctgtgtc agtgaaatcg gtgaactaca aaattgggaa gaatgcttgc tggcttcaca
     1261 agcggtcagc cggggagatg aaccaacgcc gatgatcctc tgtggcgatc gtctttattt
     1321 gaatcgcatg tggtgtaacg agcgcacagt ggcacgcttt ttcaacgaag tgaatcatgc
     1381 cattgaggtt gatgaagctc tactggcgca aaccctggac aaactttttc cagtaagcga
     1441 tgaaattaac tggcaaaaag ttgcggcggc agtggcgctg acgcggcgga tttcggtgat
     1501 ttccggcggt cctggcaccg gtaaaacgac caccgtagcg aagttgctgg cagccttaat
     1561 tcagatggcc gacggcgaac gctgccgtat ccgtctggct gcaccaacgg gtaaagctgc
     1621 cgcgcgctta accgaatctc tcggcaaggc tttgcgacaa ttaccgctga ccgatgaaca
     1681 aaagaaacgc attccggaag atgccagcac tttgcaccga ttgctgggtg cgcagccggg
     1741 tagccagcgt ttacgtcatc atgccggtaa cccgctgcat cttgatgtgc tggtggtaga
     1801 tgaagcgtca atgatcgatc ttcctatgat gtcgagactg atcgacgcct tgcccgatca
     1861 tgcgcgagtg atctttctcg gcgatcgtga tcaactggcc tcggttgagg ctggggnnnn
     1921 nnnnnntctt tgcgcgacag cctctgcctg ctgcaaaaaa gctatcgttt cggcagcgat
     1981 tctggcattg gtcagttagc tgcggcgatt aaccgtggtg ataaaacggc agtgaaaacc
     2041 gtttttcagc aggattttac tgatatcgaa aaacgacttt tacagagcgg cgaagattat
     2101 attgcgatgc ttgaggaagc tcttgcgggt tacggacgtt atctggatct gctgcaagcg
     2161 cgtgccgagc cggatttaat cattcaggcg ttcaatgagt accagctttt gtgcgccctg
     2221 cgggaaggac cgtttggcgt ggctggattg aatgagcgaa ttgagcagtt tatgcaacag
     2281 aagcgcaaaa ttcatcgtca tccgcactct cgttggtacg aaggccgacc ggtgatgatt
     2341 gcccgtaatg acagcgcgct tgggttgttt aatggcgata ttggtattgc gctggatcgc
     2401 gggcagggga cgcgcgtctg gtttgcgatg ccggacggca atattaagtc tgtgcaaccg
     2461 agtcgcctgc cagagcacga aacgacgtgg gcgatgacgg tacataaatc gcagggatcg
     2521 gagttcgacc atgcggcgtt gattttaccg agtcaacgca cgccggtagt aacgcgagag
     2581 ctggtttaca ccgcggtgac ccgcgcgcgt cgccgtctgt cgctgtatgc cgatgagcgc
     2641 atattaagtg cggcaatcgc cactcgtact gagcggcgca gtggtctggc ggcgttgttt
     2701 agttcacggg gataaacgta attgcctgat gcgacgcacg agtgttacgc atgtcgcatc
     2761 cggcgatttt catcgcttac cctaaatccg ccatcaaaac tttggattta cgctggtagt
     2821 tgtacaactg ctttttgctc tcgggcagta aatcaatatc cactggggta aatccacgtt
     2881 cctggaacca gtgaatactg cgcgtggtca gcacaaacaa tttgcttaag ccgctctgct
     2941 tcgcctgagc ggcaatgcgt tccagcagaa cctcgccccg tgatgaactg cggtaatccg
     3001 ggtgaactgc cacacaggcc atttccccaa tcttctcttc cgggaacgga tagagcgcgg
     3061 cgcaggcaat agtcgtgtta tcgcgctgaa taatggtgaa tttgtcgatt tccatctcca
     3121 gctgctcgcg agaacggcgt accagaatac cttgttgctc cagcgggcga atcaactcca
     3181 gaataccgcc aatatcgttg attgttgcgc gacgaatctg ctcggcgctt tccatcacaa
     3241 tctgcgtacc gataccgtcg cgtgagaaca actcttgcaa cagcgcgcca tcttcctgat
     3301 aactgattaa atgacagcga cgcacgccgc tgcggcaggc tttcactgcg ccacgcaaaa
     3361 agcgcaccgt accggagttg taatcgcctt tctcttcctg ggcttctacc cgtgcttgcg
     3421 cttcgttagg gaaaagttcg gagacaatat caccgtcgtc attagtgacg ccctgggaag
     3481 agcaaaaacc aatcattttt tcagctttca gtttgatggc cagttgagtg gcaatctctt
     3541 ccgaggtcag attaaagctc tcgccagtga ccgaaacagc gaccggcccc attagcacta
     3601 ttgcaccgct gtccagttga cgatggatcg cgtcttcatc aatccgccgg atacgcccgc
     3661 tatgacagta atccacgccg tcatcgacgc ccagcggctg ggcaataata aaattaccac
     3721 tgacgacgtt gatatgcgcg ccctgcagcg gtgtgttatt gagactcatc gacaggcgag
     3781 cagtaatatc cagttgcaat gttcccgcag cctgcttcac cagttccagc gtttgggcgt
     3841 cggtcacacg tatattcttg tgatacagcg gttcgtggtg atgtgcagcc agatttgcgt
     3901 cgatctgcgg acgtgcgcca tagaccacca ccagacggat gccgaggctg tgcaacaacc
     3961 cgatatcatt aacgatactg gagaaattct catgctcaat ggcttcaccg ccgagcatga
     4021 tgacaaacgt ttttccccgg tgggtattga tatagggaac cgaatggcgg aatccctcga
     4081 ccagctcggt tttacgttcc tttaccacgg tacacctctt tgcatgatta ttcgaaatta
     4141 ttgtattttt attctgtttt ttcgcggggt gcaagtgtaa attttatgcg ggagcgagtt
     4201 ttttatcagt aataccgtga atataaaaag aatgtttacc gtttnnnnnn nnnngattat
     4261 gcgtctttcg ctaaagtttc cggtcaaatt ggtcgtttac ttgttacaca gcttagattt
     4321 cttttgctcg ggagaggcat gtcaggatcc aacactgcaa tcagccgtcg tcgtttactg
     4381 caaggcgcgg gtgccatgtg gctattgagc gtaagtcagg tcagcctggc tgcggtcagc
     4441 caggtcgtgg cggtgcgcgt ctggcctgcg tccagctaca cccgcgtgac ggtagaatca
     4501 aatcgtcagc tgaaatataa gcagttcgcg ttgagtaatc ctgaacgcgt ggtggtggat
     4561 atcgaagatg taaacctgaa ctcggtgctc aaggggatgg ctgcgcaaat ccgcgctgac
     4621 gacccgttca tcaagtcggc gcgcgtcggg caatttgacc cgcaaaccgt acgtatggtt
     4681 tttgaattaa agcaaaacgt aaaaccgcag ctgtttgccc ttgcgccggt cgccgggttt
     4741 aaagagcgtc tggtgatgga cctctatccg gccaatgcac aggatatgca ggacccgctg
     4801 ctggcgctgc tggaggatta caacaaaggc gacctcgaaa agcaggtgcc gccagcacaa
     4861 agtggtccac aaccgggtaa agctgggcgg gatcgtccga ttgtcattat gcttgaccct
     4921 ggccacggtg gcgaagactc cggtgcggtg gggaaataca aaacgcgcga aaaagacgtg
     4981 gtattgcaaa tagctcgccg tctgcgctct ctgatcgaga aagagggcaa tatgaaggtg
     5041 tacatgacgc gcaatgaaga catcttcatt ccgttgcaag tgcgcgtagc aaaagcccag
     5101 aaacagcgcg ctgacttgtt tgtttctatc catgccgacg cctttaccag tcgccagccg
     5161 agcggttcct cggtgtttgc gctctcaacc aaaggcgcaa ccagtactgc ggcaaaatat
     5221 ctggcacaaa cccagaacgc ctcggacttg attggtggcg taagcaaaag cggtgaccgc
     5281 tatgtcgacc acaccatgtt cgatatggtg cagtcgctga ccattgccga cagccttaag
     5341 tttggtaaag cggtgctgaa taagctcggt aaaatcaaca agctgcataa aaatcaagtt
     5401 gaacaggccg ggtttgccgt actaaaggca ccagatattc cctccattct ggtcgaaacg
     5461 gcgtttatca gtaacgttga ggaagagcgt aaactgaaaa cggcgacttt ccagcaggaa
     5521 gttgcggagt ctattcttgc gggaattaaa gcgtattttg ccgatggggc gacgctggcg
     5581 agaaggggat aatgttttta tttattcaat
//
LOCUS       DAWDRC010000223         5607 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6491_length_5607_cov_2.449388, whole genome shotgun sequence.
ACCESSION   DAWDRC010000223 DAWDRC010000000
VERSION     DAWDRC010000223.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_5607_cov_2.449388"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1071..1080
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1494..1503
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2703..2712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4855..4864
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1249 a         1444 c         1451 g         1423 t
ORIGIN      
        1 cgcaaaagcg acagacacag cactggcacc gatatctgtt tcagtgcgaa cgcgtttcgc
       61 cacagagaaa gatttctgaa acatgcgttc cagttcgctg gccttcatat gacctttttg
      121 cgaatcggca aacgcttttt tcacctgacc gaagatctgc ggctcgccaa ggacgagaga
      181 atcaagccca ctggcaacgc gcattaaatg actgaccgca tcgttatcct gatgccagta
      241 gaggctatta cgcagatcgt cttcgttgag attatggtaa tcacacaacc agcgaatgag
      301 cgcctcttgc aggtcatccc gttcttcaac gctgagataa agttctgtgc ggttacacgt
      361 cgacagcacc acaccgccct gcaccatcgg ctgcgcgagc agactgtcaa gcgcctgatc
      421 gagcttatcc ggcgaaaacg atacacgttc tcgcagcgat acaggtgccg ttttatggtt
      481 gataccgagt gctaaaaggg tcatgtctgc gggaaataat accaacgttg atagggttag
      541 tctgcttgca tcatacagga tgcgtaagat caataaaaga gagcgccccc ttttggagta
      601 attgccgaaa gccgttagat tctggcaatt aagacaacgt gaacatagac gatggctaac
      661 ggtaacgcta gcattaaggg ttataactgc aacgtatctc aaggacttgt cattattatg
      721 cccctgcccg attttcgtct tatccgcctg ctaccgctgg ctgctcttgt gctcactgcc
      781 tgttctgtta ccacacccaa aggcccaggc aaaagcccgg attcgccaca atggcgtcag
      841 catcagcaag acgtgcgcaa tcttaatcag tatcaaactc gcggcgcgtt cgcttatatt
      901 tctgaccaac aaaaagtgta cgcccgtttc ttctggcagc aaactggcca ggatcgctac
      961 cgtctgctgc tgactaaccc attgggcagc acggaactgg agctgaatgc tcaaccgggt
     1021 aacgtgcagt tagtcgacaa taaaggtcag cgttataccg ccgatgacgc nnnnnnnnnn
     1081 gaaatcacct acagccagaa tggcaaaaac tggaaagttg tttacggtgg ttatgacaca
     1141 aaaactcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
     1201 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
     1261 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
     1321 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
     1381 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
     1441 cgaggttgtt gatgaaaact gcggcagaca gcgggcgtct ttcgacggga agcnnnnnnn
     1501 nnncggtgcg aatatcagca ttgacaagcg tttgccgatg ggcggcggtc tcggcggtgg
     1561 ttcatccaat gccgcgacgg ttctggtggc gttaaatcat ctctggcaat gcgggctaag
     1621 catggatgag ctggcggaaa tggggctgac gctgggcgca gatgttcctg tctttgttcg
     1681 tggtcatgcc gcgtttgccg aaggcgttgg tgaaatacta atgccggtgg atccgccaga
     1741 gaagtggtat ctggtggcgc accctggtgt aagtattcca acaccggtga tttttaaaga
     1801 tcctgaactc ccgcgcaata cgccaaaaag gtcaatagaa acgttgctaa aatgtgaatt
     1861 cagcaatgat tgcgaggtta tcgcaagaaa acgttttcgc gaggttgatg cggtgctttc
     1921 ctggctgtta gaatacgccc cgtcgcgcct gactgggaca ggggcctgtg tctttgctga
     1981 atttgataca gagtctgaag cccgccaggt gctagagcaa gccccggaat ggctcaatgg
     2041 ctttgtggcg aaaggcgtta atctttcccc attgcacaga gccatgcttt aagccgggca
     2101 agctgagttt cggtgacaac gtcaccttgt tccagacgtt gcatcgcgct ctttaataca
     2161 ccgcctggaa agaatcatgc ctggcccgca cagttttcgg cagattcttt ccaccaatgg
     2221 acgcatgcct gaggttcttc tcgtgcctga tatgaagctt tttgctggta acgccactcc
     2281 ggaactagca caacgtattg ccaaccgcct gtacacttca ctcggcgacg ccgctgtagg
     2341 tcgctttagc gacggcgaag tcagtgtaca aattaatgaa aatgtacgcg gtggtgatat
     2401 tttcatcatc cagtccactt gtgcccctac taacgacaac ctgatggaat tagtcgttat
     2461 ggttgatgcc ctgcgtcgtg cttccgcagg tcgtatcacc gctgttatcc cctactttgg
     2521 ctatgcgcgc caggaccgtc gcgtccgttc cgctcgtgta ccaatcactg cgaaagtggt
     2581 tgcagacttc ctctccagcg tcggtgttga ccgtgtgctg acagtggatc tgcacgctga
     2641 acagattcag ggtttcttcg acgttccggt tgataacgta tttggtagcc cgatcctgct
     2701 ggnnnnnnnn nnaacgatac cgatatggca atcatcgaca aacgtcgtcc gcgtgcgaac
     2761 gtttcccagg tgatgcatat catcggtgac gttgcaggtc gtgactgcgt actggtcgat
     2821 gatatgatcg acactggcgg tacgctgtgt aaagctgctg aagctctgaa agaacgtggt
     2881 gctaaacgtg tatttgcgta cgcgactcac ccgatcttct ctggcaacgc ggcgaacaac
     2941 ctgcgtaact ctgtaattga tgaagtcgtt gtctgcgata ccattccgct gagcgatgaa
     3001 atcaaatcac tgccgaacgt gcgtactctg accctgtcag gtatgctggc cgaagcgatt
     3061 cgtcgtatca gcaacgaaga atcgatctct gccatgttcg aacactaatc gaacccggct
     3121 caaagacccg ctgcggcggg tttttttgtc tgtaatatcc atttgtatga cctatgcctc
     3181 cttcacctgc catttagttg acagatgatg cgctcacgga tgaaacatta ttgtgaacaa
     3241 aatattttcc tcacatgtga tgcctttccg cgctctgatc gacgcttgct ggaaagaaaa
     3301 atatactgcc gcacggttta cccgtgacct gattgccggg ataaccgtcg ggattattgc
     3361 tatcccgctg gcgatggcgt tggctattgg tagtggtgtg gccccccagt acggtttata
     3421 taccgcagct gttgcgggga ttgtcattgc tctgacgggg gggtcacgct ttagcgtttc
     3481 cggtccgact gcggcatttg tggtaattct ctatcccgtt tcgcaacagt ttggactggc
     3541 aggactgctg gttgcgacct tgctgtcggg gatctttttg attctgatgg ggctggcacg
     3601 ctttggtcgc ctgattgagt atattccggt ttccgtcacc ttaggtttca cctcgggtat
     3661 cgggatcacc atcggtacca tgcagattaa agattttctc ggtctgcaaa tggcccatgt
     3721 cccggaacat tatctacaaa aagtcggcgc attatttatg gcgctgccga ccattaatgt
     3781 gggtgatgca gccattggca ttgtgacgct tggcattctg gttttctggc cgcgtctggg
     3841 catacgttta cccggccacc ttccggcctt gctggctggt tgcgcggtga tggggattgt
     3901 taacctgctc ggcggacatg ttgctaccat cggttcgcaa ttccactacg tcctggccga
     3961 tggttctcag ggtaacggta ttccgcaact gctgccgcaa ctggtgctgc cgtgggatct
     4021 gcctaattca gaattcacgc taacctggga ttctattcgc accctgctgc ctgcggcatt
     4081 ctcaatggca atgctcggcg caatcgaatc tctgctctgc gccgtggtgc tggatggtat
     4141 gaccgggacg aaacacaagg cgaacagcga actggttgga cagggactgg ggaatattat
     4201 cgctccgttc tttggtggta ttaccgctac agctgccatc gcgcgttctg ccgctaacgt
     4261 ccgtgccggg gcaacttccc ctatctcggc ggtgatccac tctattctgg ttattcttgc
     4321 cctgctggta ctggcaccgc tgctctcctg gctgccgctt tccgccatgg cagccctgct
     4381 gttgatggtg gcgtggaaca tgagtgaagc gcacaaagtg gtcgacttgc tgcgtcatgc
     4441 accgaaagat gacatcatcg tcatgctgct gtgcatgtcg ctgaccgtgc tgttcgatat
     4501 ggttattgcc atcagcgtgg ggatcgtgct ggcatcgctg ctgtttatgc gtcgtatcgc
     4561 acgtatgact cgcctggcac cggtagtcgt agacgttcca gacgatgtcc tggttctgcg
     4621 cgttattggc ccgctgtttt ttgctgccgc agaaggctta ttcacggacc tggagtcacg
     4681 tcttgaaggc aaacggattg tgattctgaa gtgggatgcc gttccggtac ttgatgctgg
     4741 tggtcttgat gcgttccagc gttttgtgaa gcgtctgccc gaaggatgtg aactgcgcgt
     4801 gtgcaacctg gaattccagc cactgcgcac tatggctcgc gcaggcattc aaccnnnnnn
     4861 nnnncgatcc cgggacgcct cgcattcttc ccgaatcgtc gcgcggcgat ggcggattta
     4921 taagtacgag attgaccagt cagcgcaaac tgactcgtca gcaaactgca ttatttgtta
     4981 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtggcgaa cccaccagta
     5041 acggtcgcac acctgttcat gcccgccaac acgggcacct gccagccaga gaatcgcacc
     5101 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
     5161 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
     5221 cacgttaccg agtaacgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
     5281 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtaggtta
     5341 tgtgcgggtg tgatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
     5401 gaacaccttg actttctaat agtccactca ggcaataatt gcattatctg catttgatac
     5461 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
     5521 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
     5581 cgccgcaacg cgacataatt ctggtgc
//
LOCUS       DAWDRC010000224         5603 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6499_length_5603_cov_2.372386, whole genome shotgun sequence.
ACCESSION   DAWDRC010000224 DAWDRC010000000
VERSION     DAWDRC010000224.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6499_length_5603_cov_2.372386"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1204..1213
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2847..2856
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1257 a         1430 c         1515 g         1381 t
ORIGIN      
        1 gtgtggctga tatgctgccc gctactttta taccctaaga aaggaaatac gatgaccacc
       61 ccaacttttg acaccatcga agcgcaagca agctacggca ttggtttgca ggtagggcaa
      121 caactgagtg aatctggcct ggaagggctg ctgccagaag ctctggttgc aggcattgcc
      181 gatgcgctgg aaggcaaaca tccggctgtt ccggttgatg tggtgcaccg cgcgctgcgt
      241 gaaatccacg aacgcgccga tgccgttcgt cgtcagcgtt tccaggcgat ggctgctgaa
      301 ggtgtgaaat acctggaaga aaacgccaaa aaagaaggtg taaatagcac cgaatctggc
      361 ctgcaattcc gcgtgatcaa ccagggcgaa ggcgcaattc cggcacgtac cgaccgcgtt
      421 cgtgttcatt acaccggaaa actgatcgac ggcaccgtgt ttgacagctc cgttgctcgc
      481 ggcgagccag ctgaattccc ggttaacggc gtgatccctg gctggattga agcactgact
      541 ctgatgccgg taggttctaa atgggaactg actatcccgc aggaactggc atatggcgag
      601 cgtggcgcag gcgcgtccat ccctccgttc agcaccctgg tgtttgaagt cgaactgctg
      661 gaaatcctct aagcagcgca ttctgttccc ctcgagccag aggggagcag gcattcagca
      721 ataaaccctt cagtttgcca aacggcgcta ttttatgttg caaagcccct gtaagcgtgt
      781 atttttgtga gctgtttcgc gttatcaccg tgatatgaca ctcactttaa acataaaatt
      841 aacattagat ctaaatctta gtattcatcc cgcgtattgt tacctaatat cgatgagtcc
      901 cgatacagat tcgtcgtatc atagactgac taaaggccgt agagcctgaa caacacagac
      961 aggtacagga agaaaaaaac atggtagatc aggtaaaagt cgttgccgat gatcaggctc
     1021 cggctgaaca gtcgctacgg cgcaatctca caaaccgaca tattcagctt attgccattg
     1081 gcggtgccat tggtacaggg ttgtttatgg ggtccggcaa aacgattagc cttgccgggc
     1141 cgtcgatcat tttcgtttat atgatcatcg gttttatgct ctttttcgtg atgcgggcaa
     1201 tggnnnnnnn nnnggaattg ctgctttcga atctggaata caaatctttt agtgacttcg
     1261 cttccgattt actcgggccg tgggcaggat atttcaccgg ctggacttac tggttctgct
     1321 gggttgtaac cggtatggca gacgtggtgg cgatcacggc ttatgctcag ttctggttcc
     1381 ccgatctctc cgactgggtc gcctcgctgg cggtgatagt gctgctgctg acgcttaacc
     1441 tcgccaccgt gaaaatgttc ggtgagatgg agttctggtt tgcgatgatc aaaatcgtcg
     1501 ccattgtgtc gctgattgtc gtcggcctgg tcatggtggc gatgcacttt cagtcaccga
     1561 ccggtgtgga agcgtcattc gcgcatttgt ggaatgatgg cggctggttc ccgaaaggtt
     1621 taagtggctt ctttgccgga ttccagatag cggttttcgc tttcgtaggg attgagctgg
     1681 taggtacaac agctgcggaa accaaagacc ctgagaaatc actgccgcgc gcgattaact
     1741 ccattccgat ccgtatcatt atgttctacg tcttcgcgct gattgtgatt atgtccgtga
     1801 cgccgtggag ttcggtagtc ccggagaaaa gcccgttcgt tgaactgttc gtgttggtag
     1861 gtttgcctgc ggctgccagc gtgatcaact ttgtggtgct gacctctgcg gcgtcttccg
     1921 ctaacagcgg tgtcttctct accagccgta tgctgtttgg tctggcccag gaaggtgtgg
     1981 caccgaaagc gttcgctaaa ctctctaagc gcgcagtacc cgcgaaaggg ctgaccttct
     2041 cctgcatctg cctgctcggc ggcgtggtga tgttgtatgt gaatcccagc gtgattggcg
     2101 cgttcacgat gattacaacc gtttccgcga ttctgtttat gttcgtctgg acgattatcc
     2161 tttgctcgta cctggtgtat cgcaaacagc gtccgcacct gcatgagaaa tcaatctaca
     2221 agatgccgct cggcaagctg atgtgctggg tatgtatggc gttctttgtg tttgttctgg
     2281 tgttgttgac actggaagat gacacccgcc aggcgctgct ggttaccccg ctgtggttta
     2341 tcgcgctggg gctgggctgg ctgtttattg gtaagaagcg ggctgctgaa ctgcgaaaat
     2401 aaccgcatta tcatgctgga tggcgcaatg ccatccagct tttagatcac tcacccgcca
     2461 gcgcgcgcgg gaacagcaca ttgttttcca ggctgatatg ctccatcagg tcatcaatta
     2521 gttcattaat gccgttatac atcgctttcc aggtggtgca ggcttctggc ggcggtgtga
     2581 cgttattggt ggtgtgttta attacttcca gcagttcgcc cgcttcatcg tgctcgcttt
     2641 ccattacgct gattggcccc attgcctggc tgcccatgcc ttgtttgatc attgggaaga
     2701 ggatttgctc ttctttcatc atgtggctgg aaagctcttc atgcagcata gtcaggtatt
     2761 ttgtcatccc ttttggcaca cttggtttgt cggcgtgaac gcgctcgact ttagtcgctt
     2821 gcaggatcag ctccggcagt tgctcgnnnn nnnnnntgat atggtcgatg atttctgcca
     2881 gcggggcgct acgccagtct ttctcaatcg gttgttcagc gagctttgcc agctcagctt
     2941 caatgacctc aacatccagc tctttacgtg ccgccgcacg cgccagcgtt tgcttaccgc
     3001 cgcagcagta atccatatca tatttacgaa acagagccga agcgcgagga atagagagcg
     3061 ccagttcacc taaaggttgg tcgcgataag ccatagctga tacctcattc ttaataagat
     3121 aagatgcatt ttaaatgcat ctttaaggca aaagctataa cccttacgta gcagaggctt
     3181 atttaactca ctgccagcgt gtcaactttc tgagacagat tcagttaaaa gtttttctgg
     3241 ttttgctctg accgcgatga ccacgccaat cactaacagc gcgataccgc tcagcgtcat
     3301 tagcggcggc atttgctggc ggagtaaaaa ggtgtacagc aaacctgcca gcgtttcgaa
     3361 aacaatcagc ggcccgagaa tcactgtcgg taatcgctgg ctggcgacgt tccagcagag
     3421 tgcgccaacc catgagcaaa gcacggctat cgcaaccatc agactaataa acaccagcgg
     3481 acgggggcca aagggtaagg agaagtccgg cgtttgcata ttcagccagt aacaggcgac
     3541 gagatagccg atgagagaaa ccggcagcgt gaccagcgcc tgcgccgtcg cccacatcat
     3601 cggatgtttg tcgggatttt cccgcagcca gcgggcgttg cgcagggcat accatgccca
     3661 gcagaccacg gaaactaacg ccagcacgat gcctgaggta taacgtgccc agtcaaaatc
     3721 ggggagtccg tggtttaact cagcaatatt cacacacgcc aggccgatgc caatacaaac
     3781 cagtgccggg gcgagttttc cccatgcgag tttgccgtcg cgctggctat aaagcagatt
     3841 ggcaaaaacg ggaatcacca ccggcagggt gccgataatc atcgtggaaa caggcgcgcc
     3901 agtacgttga atggcactgg caaggcagaa gtaatagata aggttgccca tcatggtgag
     3961 catcaaggcg gtaagccagt cgcgacgcgc caactgacgc agacgcacgc gtcccagcca
     4021 ggcaatgggc agcgcaatta accctagcgc caggtaacgc cccatcgact gtaacatcgc
     4081 cgggtattcc ggcacgatca acggcccgac aaaaataagc ccccacatca accctgctaa
     4141 cagggcgtac agcacgccgc taatcatcac tggcatccac tggtctgtca gaagaaatat
     4201 tcaggaagca gacatgattg ctcccggcat cccgtcatta tcagagagga tgcgggagga
     4261 tgccacttta attaactagt tattaacatt aaaaagatgg cttacgcttt cggctaacgt
     4321 tgtcgttggg cggccaatca atttgctaag cgttttgctg tcatcaaaca gaccgccttt
     4381 cgatgcgcca acgtcagaat ccgccagcat atccgccagt ccatcaggca gtccgacgct
     4441 tttcagtgcc gcagcgaaat cggcttcgct caggttttga taggtaatcg gtttgccgct
     4501 ctgtttggtg agttctgccg ctaactgtgt cagtgtccag gcactatcgc ccgccagttc
     4561 gtaaaccttg ccttcatgac cggcttcgct aattacgcgc gccgcagcta ccgcataatc
     4621 agcccgcgtt gctgaggcaa ttttgccatc gcccgcggca ccgataaata cgccgtgttc
     4681 cagtgctgcc ggggcgctgg cgaggtagtt ttcggtgtac cagccgttgc gcagcagagt
     4741 gtaaacgatg ccagaatcag ccagcatttt ctccgtctcg atgtgctcat cggcgaggcc
     4801 gagcggggag gaatcggcat gtagcaggct ggtataagcg ataaatttca catcggccgc
     4861 ctttgcggca ttaataacat tacgatgctg cggggcacgt tgacccactt cgctggaaga
     4921 gatcagcagt agtttttcca ctccctgaag cgcagatgtc agtgcggctt catcgccgta
     4981 atcagcctga cgcacggtaa tgccttgtgc tgtcagtacc tgtgcttttg ccggattacg
     5041 aacgatagcc actatttggc tggcaggaac cgttttcatc aaggattcaa taacatagtg
     5101 accaagttgg cctgtggcac cagtaatagc gatcatggga agtctccatc gttttgtctt
     5161 gtgtttaaat acgctaacac ttaaacttac ttttagtaag tacgtacaaa aaggtaagta
     5221 tgaaatgagt caggttagcc tgtcgcaaca actcaaagag ggaaatctct ttgcggaaca
     5281 gtgcccgtcg cgcgaggttt tgaaacacgt caccagccgt tggggggtgt tgattctggt
     5341 ggcgctacgc gaaggtactc atcgctttag cgacctgcgg cgcaaaatcg gcggggtgag
     5401 tgaaaagatg cttgcgcagt cgttacaggc gttagagcag gatggttttc ttaaccgtat
     5461 cgcgtatccg gtggtgccgc cgcatgtgga atacagcctc acgccgctgg gcgagcaggt
     5521 gagcgaaaag gttgctgcac tggcggactg gattgagttg aatttgcccg aggtgttagc
     5581 ggtgcgggat gaacgtacgg cat
//
LOCUS       DAWDRC010000225         5599 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6504_length_5599_cov_2.043290, whole genome shotgun sequence.
ACCESSION   DAWDRC010000225 DAWDRC010000000
VERSION     DAWDRC010000225.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6504_length_5599_cov_2.043290"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    218..227
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1113..1122
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1961..1970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3776..3785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1313 a         1241 c         1485 g         1520 t
ORIGIN      
        1 tcggtgattc catcccgaat gacagcgcgc tgaacggtaa aaatgtctgg ctgggtgata
       61 tttcccgggc agttgcgcac cctgagttgt tcctgagtga agccaaaggc catgcgaaac
      121 cttacactgc gccggtcgct gcgacagcac cggttgccgc cagcggtccg aaacgcgtag
      181 ttgcggtgac tgcttgcccg actggcttag cacccacnnn nnnnnnngcg cgggtaatgc
      241 aatcacccct gaagaagtcg cagcagcgga tctggtgatt gtggcggcag atatcgaagt
      301 agatctggcg aaatttgctg gtaaaccgat gtatcgcacc tctaccggtc tggcgctgaa
      361 gaaaactgcg caggaactgg ataaagcggt tgctgaagca acgccgtatg aaccggcggg
      421 caaaactcaa acagcgacca ctgaaggtaa gaaagagagt gcaggtgctt accgtcactt
      481 gctgacgggc gtctcttaca tgctgccgat ggttgttgca ggtggtctgt gtatcgcgct
      541 ttcttttgct tttggtatcg aagcgtttaa agagccgggt acgttggcag cggcgctgat
      601 gcagattggt ggtggttcag cctttgcgct gatggtgccg ctactggcag gttatattgc
      661 cttctccatt gccgatcgtc cgggtctcac gccgggtctg attggcggta tgctggcggt
      721 tagcaccggt tctggcttca ttggtggtat tattgcgggc ttcctggcgg gttacattgc
      781 gaagttaatc agtacgcaac tgaaactgcc acagagtatg gaggcactga aaccgatcct
      841 gatcattccg ctaatttcca gtctggtggt cggtctggcg atgatctacc taatcggtaa
      901 accggttgct ggcattctcg atgggttgac tcactggctg cagaccatgg ggactgcgaa
      961 tgcggttctg ctgggggcga tcctcggtgg catgatgtgt actgacatgg gcggtccggt
     1021 aaacaaagca gcgtacgcat tcggtgtggg tctgctgagt actcaaacct atggcccgat
     1081 ggcggcgatt atggcggcag gtatggtgcc acnnnnnnnn nntggaaatg ggtctggcaa
     1141 caatggtggc gcgtcgcaaa ttcgacaaag cgcagcagga aggtggcaaa gccgctctgg
     1201 tacttggtct gtgctttatt tcggaaggtg caattccgtt tgctgctcgt gatccgatgc
     1261 gtgtgctacc gtgctgtatc gttggtgggg cgctgactgg cgcaatctca atggcgattg
     1321 gtgcgaaact gatggctccg cacggtggtc tgtttgttct gctgatccct ggcgctatta
     1381 cgccggtatt gggttacctg gtagcaatta ttgccggtac gctggtggcg ggtttggcct
     1441 atgccttcct caaacgcccg gaaacacaaa tagtagaaaa aaacgcataa tttctcttca
     1501 tcatcggggc tgagacatct ctgccccgat gatgacaaac gcaaaattgc ctgatgcgct
     1561 atgcttatca gacctacatg aaccctgcaa tatattgaat ttttgcgctt ttgtaggtcg
     1621 gataaggcgt ttacgccaca tccagcatga acaaagcgcg cattgtcaac aatctgaggg
     1681 cagaaacatc tctgcccttt ttcttgcctg ccatattgtg tgttacacat cacaactttc
     1741 tcttttgttg ctgcataaat agacaaactt tgtgaccgcc ttcatatctg gccgatttcg
     1801 ttttttctga tcgatttaca ttacgttaaa catgcttatt atgaacatgt gtttaaatct
     1861 gaacacgtgc tctaatagtc ttattgactg taatgactat tttacccaat ttcttcgttg
     1921 gcaatttatt gccagggggc gtgctatgcg cgaaaaggac nnnnnnnnnn catagtaatt
     1981 attggctcgg cgaatattga tgtggctggg tattcacatc atccattgaa ttatgcggat
     2041 tcgaatcccg gaaaaattaa atttacccca ggtggcgtgg gaaggaatat cgctcataat
     2101 ctggcgttac tggggaaaaa tgcctggctt ttaagtgcag taggtggtga tttttatggg
     2161 caatcattac tggcgcaaac taatcaatcc ggtgtttatg tcgataaatg cttaattgtt
     2221 ccgggtgaga atacatccag ctatttatca ctgctcgata atacaggaga aatgcttgtc
     2281 gcgataaatg atatgagtat tagtgactgt atttctgcag aatttcttgc ccagcatcag
     2341 gagtttattc gcggtgcaaa ggttattgtt gcggattgca accttagtga agaggcgctg
     2401 gtctgggttc tggaaaatag cggggaaacg cctgtattta ttgatccggt atcggcatgg
     2461 aaatgcgtca aaatccgcga tcatctgagc aaaatccata cgttaaaacc taaccgtctt
     2521 gaagccgaaa cgttgagcgg aattgcactt tccggacgtg aagatgtcgc aaaggtcgcc
     2581 gcctggtttc atcaacacgg cctgaaccgc ctggtgctga gcatgggagg ggatggcgtt
     2641 tactacagtg atatcaacgg tgaaagtggg tggtcggccc ctattgaaac gaatgtcatc
     2701 aatgttactg gggcaggcga cgccatgatg gcggggctgg cctcctgttg ggtagacggt
     2761 atgccgttta tcgattctgt tcgatttgcg cagggatgtt cgtcaatggc acttgcctgt
     2821 gaatatacca acaaccctga attatcaatt gccaatgtta catcgttagt ggagaacaca
     2881 gaatgtctga attaactctg tcccctgaat tattacaaat atccgctgaa gtacaggatg
     2941 ctttaaaaaa taaaaaaccg gttgttgcgc tggaatcgac cataatttct cacggaatgc
     3001 cattcccaca aaatgcccaa accgcaattg aagtagaaga aacaattcgt aaacagggcg
     3061 cagtaccagc cactatcgcc attattggcg gtgtgatgaa agtgggctta agtaaagaag
     3121 aaattgaatt gctgggccgt gaaggacata acgtgacgaa agttagtcgt cgtgatttac
     3181 cttttgtcgt tgctgcaggg aaaaatggcg cgactaccgt tgcatcaacc atgattattg
     3241 cagcactggc tgggattaaa gtttttgcaa ccggaggtat tggtggtgtg catcgcggag
     3301 cggaacatac cttcgatatt tctgccgatt tacaggaact ggcaaatacc aatgtcactg
     3361 ttgtttgtgc gggggcaaaa tctattctcg atttaggatt aacaacagaa tatttagaaa
     3421 ccttcggtgt accgttaatt ggttatcaga caaaagcgct gcctgcgttt ttctgccgaa
     3481 ccagcccatt tgaagtcagc attcgtctcg acagtgcccg cgaaattgct cgcgcgatgg
     3541 cagtgaagtg gcaaaccggt ttgaacggcg gcctggtggt agcgaacccg atcccggaac
     3601 agtttgcgat gccagaagaa accatcaatg cagcaattga tcaagccgtt gctgaagccg
     3661 aagaacaggg tgttattggg aaagagagta caccgttcct gctggcgcgt gttgcggagc
     3721 taaccggcgg tgatagcctg aaatccaata ttcaactggt ctttaataac gccatnnnnn
     3781 nnnnntattg ctggcaattg cctttgtgtt gtcggtaaat aaaaagagca tcagcttgcg
     3841 cacggttggg gctgcgctgc tgctgcaaat tgccattggt ggcatcatgc tctattttcc
     3901 cccgggaaaa tgggcagtag agcaggcggc attaggcgtc cataaagtga tgtcctacag
     3961 tgacgcagga agtgccttta tttttggttc gctggtcggt cccaaaatgg atgttctgtt
     4021 tgacggtgcg ggctttatct tcgcctttcg cgtactcccg gcgattattt tcgttaccgc
     4081 gcttatcagc ttgctgtact acattggcgt aatggggctg ctgatccgca ttcttggcag
     4141 tatttttcag aaagccctca acatcagcaa aatcgaatct tttgtcgcag tcaccactat
     4201 tttcctcggg caaaatgaga tcccggcgat cgttaaacct tttattgatc gcatgaatcg
     4261 caacgagcta tttacggcta tttgtagcgg catggcgtct attgcgggtt cgatgatgat
     4321 tggctatgcc ggaatgggcg tgccgattga ctatttgtta gccgcttcgc tgatggctat
     4381 tcctggcggt atcctgtttg cacgtattct tagtccggcg acggaacctt cgcaagttac
     4441 gtttgaaaat ctgtcgttta gcgaaacacc gccaaaaagc attattgaag ctgcggcgaa
     4501 cggtgcaatg accgggctga aaatcgccgc cggtgtggcg acggtggtaa tggcatttgt
     4561 ggcgatcatt gcgctgatca acggtattat cggcggaatt ggcggctggt ttggttttgc
     4621 caatgcctct ctggaaagta tttttggcta tgtgttggca ccgctggcgt ggatcatggg
     4681 tgtggactgg agtgatgcca accttgcggg tagcctgatt gggcagaaac tggcgattaa
     4741 cgaatttgtc gcttacctga gtttttcgcc atatctgcaa acgggcggca cgctggaagt
     4801 gaaaaccgtg gcgatcatct cctttgcgct gtgtggtttt gctaactttg gttctatcgg
     4861 tgttgtcgtt ggagcgtttt cggccatttc gccaaaacgc gcgccggaaa tcgcccagct
     4921 tggtttacgg gcgctggcgg cggcgacact ttctaacctg atgagcgcga ccattgccgg
     4981 gttctttatt ggtttagctt gagtttcatt gccggatgta cgccttatta agcgttgcat
     5041 ccggcaatcg cgctattgca aataattttt actgcatcat cccggagaat ttattctccg
     5101 ggtccatctc cagcgccagt cctgacaact gctttctgtt tttaatgaga taccctttct
     5161 tgcttttaat taataaaccg tcattaatga attgcgtgag aacatataaa agatgtcgat
     5221 aagaaacgcc taaatactct gccgcttgcg tatgcttttc gtgataaaga tcaccttcct
     5281 gcgagagtaa tataaaagct gccaggcgat tcactaacgg aaatgattga ttctgagtta
     5341 aagaaacaat attacgataa tttttatgac ttaaggtgac gcagagtttt cgtaaaaata
     5401 gcgtgtcgtt taataacaac gggcggtaat gcttcatagg gagcgcaagg caccaacact
     5461 cttcaatagc ctgcactgca cgcggttcat ggtctttgtc gattaactca atctcgccaa
     5521 taaaacaggg cgcggcaaag aaatcgatca gcgacacgcg accattagca agcgtgacat
     5581 aaagcctggc gcggcctcg
//
LOCUS       DAWDRC010000226         5595 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6507_length_5595_cov_2.452166, whole genome shotgun sequence.
ACCESSION   DAWDRC010000226 DAWDRC010000000
VERSION     DAWDRC010000226.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6507_length_5595_cov_2.452166"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3005..3014
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3596..3605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1407 a         1481 c         1479 g         1208 t
ORIGIN      
        1 ggcaattggt ctggggagca ttctgacacc atacgccacc ggacccagtc cgatttacta
       61 cggcagtggt tatctgccaa cggcggatta ctggcgactg ggggcgattt ttgggctgat
      121 attcctcgta ttgctggtga ttaccggctt actgtggatg cccgtggtgt tgctttaagt
      181 gaggacggtg ccggaaattg ctctccggca cccaatagtt ttacgcagcc ggtaactccg
      241 ccagcggcca gcgcggacgc acactcacgc cgagatccgc cgtcgcgcct gctttaaacc
      301 gcaccattcc ggcataggcg atcatcgcgc cgttatcagt acaaaattcc ggacgcgcgt
      361 agaacacttc gccacgacgc tttttcatca tttcagccag cttcgcccgc agtgtgcggt
      421 tagcactcac gccgcctgcc atgaccagtc gcttaaagcc cgtctgatcc agtgctcgct
      481 tacacttaat catcaacgta tcgaccactg catcttcaaa ggcgcgggcg atatcggcac
      541 gcgtctgatc gtcagtgccg ttgtcacgta tggtatttgc cgcgaaggtt ttcagaccag
      601 aaaagctgaa atccagcccc ggacggtcgg tcatcgggcg cgggaagaca aagcgcccgg
      661 cagtaccctg agccgccatt ttcgacagta atggcccgcc aggataatcc agccccagca
      721 gcttcgcggt tttatcaaac gcttcaccag ccgcatcatc gatagactct ccgagcagct
      781 cgtactgacc aatgccagtc acgctgatta actgcgtatg accgccggaa accagcagcg
      841 cgacaaacgg aaattccggt gggttatctt ccagcatcgg cgctaacaga tgtccttcca
      901 tatgatgtac cgggattgcc ggaacgtccc aggcaaacgc cagagaacgc cccacggtcg
      961 cgccaaccag cagtgcgccg actaatcctg ggcctgcggt ataggccaca gcatcaatgt
     1021 cttttgccgt taaaccagac tcctttagcg cctcctggat caacggtacg gttttacgca
     1081 cgtgatcgcg ggaggccagt tcaggcacga cgccgccgta gtcagcgtgc aatttcacct
     1141 gactatacaa ttggttggct aacaaacctt tttcatcgtc gtaaatggcg atgccggttt
     1201 catcgcagga agtttcaata cccagtacac gcatgacttg ttttacctcg ctttattacc
     1261 gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt tcgcccctgg agaaagcctc
     1321 gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa ggccgcggtg ctttacaaag
     1381 cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt gatgccagcg
     1441 gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac gtgaaaacga
     1501 gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag gtgttctggc
     1561 ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc gcgctaaagc
     1621 ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc gcactcgtct
     1681 gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg taaggccgtg
     1741 cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc ggggcctatg
     1801 gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga catcgtcgat
     1861 ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc gtgttgtcca
     1921 ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt ttaccactgc
     1981 tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga caagctcgag
     2041 ttcgtagaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc atttgaagca
     2101 ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca gttgatggac
     2161 ggtctgaata cgttttacca acaatcttta caacaacctg ttgccatgtc tgcgcgccag
     2221 tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat tggttttgcg
     2281 ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa tcgccagtca
     2341 ttgattgatg cgggcatgtt ggtcactaac gatcaggggc gcagttacga tcgtttccgc
     2401 gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt tggcgggcgc
     2461 gtgctgggca acgatactcc caaatacctg aactcgccgg aaacggacat tttccataaa
     2521 ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc caatcgtctg
     2581 cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat taattacgcc
     2641 gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt ccgcgcgacc
     2701 aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc ctggcgagcg
     2761 ctggaaacgg cgctgcctta catgacagac ggtcgtcagc tacgctttat gtttttgcct
     2821 gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt tgaagcgcgg
     2881 atggagcagg cgatgccact ctccgcattt ctgtttaaca gtctgatgcc gcaagttgat
     2941 ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccact gatatcgcaa
     3001 gtgcnnnnnn nnnnacgtca ggaattaggc aacaaattag gcatacttga tgacagccag
     3061 cttgaacgat taatgccaaa agcggcagag agcggcgttt ctcgccctgt tccgcagcta
     3121 aaacgcacga ccatgcgtat acttataggg ttgctggtgc aaaatccaga attagcgacg
     3181 ttggtcccgc cgcttgagaa tctggatgaa aataagctcc ctgggcttgg cttattcaga
     3241 gaactggtca acacttgtct ctcccagcca ggtctgacca ccgggcaact tttagagcac
     3301 tatcgtggta caaataatgc tgccaccctt gaaaaactgt cgatgtggga cgatatagca
     3361 gataggaata ttgctgagca aaccttcacc gactcactca accatatgtt tgattcgctg
     3421 cttgaactgc gccaggaaga gttaatcgct cgtgagcgca cgcatggttt aagcaacgaa
     3481 gaacgcctgg agctctggac attaaaccag gagctggcga aaaagtgatt taacggctta
     3541 agtgccgaag agcgatcggg aagcccccga cagccgcact gagaggcagc ggcaannnnn
     3601 nnnnnatata agtacgccct cgtaattatc gttggcggta aacaaccgtt ggatttcagc
     3661 gttaacagct gaaggacatc gggtcaatcg cccaacacca acctcatgaa ataagtgtgg
     3721 ataccgtctt atggagcaaa acccgcagtc acagctgaaa cttcttgtca cccgtggtaa
     3781 ggagcaaggc tatctgacct atgccgaggt caatgaccat ctgccggaag atatcgtcga
     3841 ttcagatcag atcgaagaca tcatccaaat gatcaacgac atgggcattc aggtgatgga
     3901 agaagcaccg gatgccgatg atctgatgct ggctgaaaac accgcggacg aagatgctgc
     3961 cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa atcgggcgca cgactgaccc
     4021 ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg ttgacccgcg aaggcgaaat
     4081 tgacatcgct aagcgtattg aagacgggat caaccaggtt caatgctccg ttgctgaata
     4141 tccggaagcg atcacctatc tgctggaaca gtacgatcgt gttgaagcag aagaagcgcg
     4201 tctgtccgat ctgatcaccg gctttgttga cccgaacgca gaagaagatc tggcacctac
     4261 cgccactcac gtcggttctg agctttccca ggaagatctg gacgatgacg aagatgaaga
     4321 cgaagaagat ggtgacgacg ataccgccga tgatgacaac agcatcgacc cggaactggc
     4381 tcgcgaaaaa tttgcggaac tgcgcgctca gtacattgta acgcgtgaca ccatcaaagc
     4441 gaaaggccgc agtcacgctg ccgctcagga agagatcctg aaactgtctg aagtattcaa
     4501 acagttccgc ctggtgccga agcagtttga ctacctggtc aacagcatgc gcgtcatgat
     4561 ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc tgcgttgagc agtgcaaaat
     4621 gccgaagaaa aacttcatta ccctgtttac cggcaacgaa accagcgata cctggttcaa
     4681 cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg cacgatgtct ctgaagaagt
     4741 gcatcgcgcc ctgcagaaac tgcagcagat tgaagaagaa accggcctga ccatcgagca
     4801 ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg aaagcccgcc gtgcgaagaa
     4861 agagatggtt gaagcgaact tgcgtctggt tatttctatc gctaagaaat acaccaaccg
     4921 tggcttgcag ttccttgacc tgattcagga aggtaacatc ggtctgatga aagcggttga
     4981 taaattcgaa taccgccgtg gttacaagtt ctccacctac gcaacctggt ggatccgtca
     5041 ggcgatcacc cgttctatcg cggatcaggc gcgcaccatc cgtattccgg tgcatatgat
     5101 tgagactatc aacaagctca accgtatttc tcgccagatg ctgcaagaga tgggccgtga
     5161 accaacgccg gaagaactgg ctgaacgcat gctgatgccg gaagacaaga tccgcaaagt
     5221 gctgaagatc gccaaagagc caatctccat ggaaacgccg atcggtgatg atgaagattc
     5281 gcatctgggg gatttcatcg aggataccac cctcgagctg ccgctggatt ctgcgaccac
     5341 cgaaagcctg cgtgcggcaa cgcacgacgt gctggctggc ctgaccgcgc gtgaagcgaa
     5401 agttctgcgt atgcgtttcg gtatcgatat gaacaccgac cacacgctgg aagaagtggg
     5461 taaacagttc gacgttaccc gcgaacgtat ccgtcagatc gaagcgaagg cgctgcgcaa
     5521 actgcgtcac ccgagccgtt ctgaagtgct gcgtagcttc ctggacgatt aatcggtagg
     5581 ccggatcagg tgtta
//
LOCUS       DAWDRC010000227         5559 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6551_length_5559_cov_1.862100, whole genome shotgun sequence.
ACCESSION   DAWDRC010000227 DAWDRC010000000
VERSION     DAWDRC010000227.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5559)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6551_length_5559_cov_1.862100"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3065..3074
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4312..4321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1250 a         1478 c         1611 g         1200 t
ORIGIN      
        1 caaaccgatt tgctgcccgc aggtgttacg cccgaaatca ttacgctttc tggcggggta
       61 ggcgaatgtt atcgccacca gcccgccgac ccgttctgtt ttgccgatat aggcccgctt
      121 ctggcaacgg cgctgcatga ccatccgcgt ctgcgtgaga tgaacgtgca gtttccggcg
      181 caaaccgtgc gcgccacggt gattggcgcg ggtgctcata ccctttcgct ctctggcagc
      241 acaatctggc tggagggcgt acaactgccg ctgcgcaatt tgccggtggc gatcccgatt
      301 gatgaaacgg atctggtcag tgcctggcaa caggcgctga ttcaactgga tctttgcccc
      361 aaaactgacg cgtacgtgct ggcgcttccc gcctcgctgc ctgtgcgtta cgccgcggta
      421 ctgacggtca tcaacgcgct ggtcgatttc gtcgcgcgtt ttccgaatcc gcatcccctg
      481 ctggtggtgg ccgggcagga ctttggtaaa gctctgggca tgttgttgcg cccacagcta
      541 caacaactcc cgttggcagt cattgacgaa gtgattgtcc gcgcggggga ctatatcgac
      601 attggtacgc ctctttttgg cggatcggtt gtgccggtga cggtgaaatc actcgcattt
      661 ccttcctgag ggaacgactt atgaaactaa agaccacatt gttcggcaat gtatatcagt
      721 ttaaggatgt aaaagaggtg ctggctaaag ccaacgaact gcgttcgggg gatgtgctgg
      781 cgggcgttgc tgcggcaagc tcacaggagc gcgtggcggc aaagcaggtg ttgtcggaaa
      841 tgaccgtagc agacatccgc aataatccgg tgattgccta tgaagatgac tgcgtgacgc
      901 ggctgattca ggacgacgtt aacgaaacgg cctacaacca gattaaaaac tggagcatca
      961 gcgaactgcg tgaatacgtc ctgagcgacg aaaccagcgt tgacgacatt gcctttactc
     1021 gcaaagggtt gacctcggaa gtggtagcgg cggtagcgaa gatttgctcc aacgcggacc
     1081 tgatctacgg cgcgaagaaa atgccggtga tcaaaaaggc caataccacc atcggtattc
     1141 cgggcacctt tagcgcccgt ttgcagccga acgatacccg tgacgacgtg caaagtatcg
     1201 ccgcgcaaat ctacgaaggg ctttcctttg gggtgggcga tgcggtgatt ggcgttaacc
     1261 cggtgactga cgacgtggaa aacttaagcc gcgtgttgga taccatttat ggcgtgatcg
     1321 acaaattcaa catcccaact cagggctgcg tactggcgca cgtcaccacc cagatcgaag
     1381 cgatccgtcg tggcgcacca ggcgggctga ttttccagag tatctgcggc agcgaaaaag
     1441 ggctgaaaga gtttggtgta gagctggcga tgctcgatga agcacgcgca gtgggcgcgg
     1501 agttcaaccg tatcgccggg gaaaactgtc tctacttcga aaccggacaa ggctcggcgc
     1561 tatccgctgg cgctaacttc ggcgcagacc aggtgacgat ggaagcgcgt aactatgggc
     1621 tggcgcgtca ttacgatccg tttatcgtca acaccgtggt cggttttatt gggccggagt
     1681 atctctacaa cgaccgccag attatccgcg caggcttaga agatcacttt atgggcaagc
     1741 tgagcggcat ctctatgggc tgtgactgct gctacaccaa ccacgctgac gctgaccaga
     1801 acctcaacga aaacctgatg atcctgctcg ccaccgcagg ctgtaactac atcatgggga
     1861 tgccgctggg cgatgacatc atgctcaact atcagaccac cgcattccac gacactgcca
     1921 ctgtgcgtca gttactcaac ctgcgcccgt caccggagtt tgaacgctgg ctggaaagca
     1981 tgggcattat ggcaaacggt cgcctgacca aacgggcggg cgatccgtca ctgttcttct
     2041 gatgacgcgg agataacaca tcatggatca aaaacagatt gaagaaattg tacgcagcgt
     2101 gatggcgtca atgggacaaa cggcccccgc gccgtcagaa gcaaagtgcg ccaccaccaa
     2161 ctgtgcggca ccggtgacct cggaaagctg cgcgctggat ttaggttctg cggaagcaaa
     2221 agcgtggatt ggtgtggaaa acccacatcg cgcggacgta ttaacagaac tgcgtcgcag
     2281 caccgtggcc cgcgtttgta ccggtcgtgc cggtccgcgt ccgcgcaccc aggcgttgct
     2341 gcgtttcctg gccgatcact ctcgttcgaa agataccgtt ctgaaagaag taccggaaga
     2401 gtgggtgaaa gcgcagggct tgctggaagt ccgctccgaa atcagcgaca aaaatctcta
     2461 cctgactcgt ccggatatgg gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc
     2521 acagtgtgtt gctaatccgg acgtgcaggt cgttatttct gatggcctgt caactgatgc
     2581 gatcaccgtg aactacgaag agatcctgcc gccgctgatg gcgggcctga agcaagccgg
     2641 gctgaaagtc ggtacgccat tctttgtacg ttatggtcgc gtgaagattg aagatcagat
     2701 cggtgagatc ctaggcgcga aagtagtgat cctgctggtg ggcgaacgtc cggggttggg
     2761 gcagtcagaa agcctctcct gctacgccgt ttactcgccg cgtatggcga ccaccgttga
     2821 ggccgatcgc acctgtatct ctaatattca ccagggcggc acgccgccgg ttgaagccgc
     2881 agccgtcatt gtggatttgg cgaaacgtat gctggagcag aaagcatccg gcatcaacat
     2941 gacccgataa ggaggcatca tgccagcttt agatttgatt cgaccgtcgg taaccgccat
     3001 gcgggtgatt gcctccgtta acgccgaatt tgcacgtgaa ctgaaattgc cgccgcatat
     3061 tcgtnnnnnn nnnncgggct gatttctgct gattcagatg acgtcaccta cattgcggct
     3121 gacgaagcga ccaagcaggc gatggtcgaa gtggtatatg gtcgctcgct gtatgcgggc
     3181 gctgcacacg gcccgtcacc gactgccggt gaggtgctga ttatgcttgg tggaccaaac
     3241 ccggcggaag tgcgtgctgg tctggatgcg atggttgcga atattgaaaa tggcactgct
     3301 ttccagtggg caaatgacgc agaaaacaca gccttcttgg cacatgtagt ttcgcgtacc
     3361 ggttcttatc tctcatcaac cgccgggatc acgcttggcg atccgatggc gtatctggtg
     3421 gcaccgccgc tggaagcgac ctacggcatt gatgcagcgt tgaaatctgc cgacgtgcag
     3481 ttggtgacct atgtcccgcc accgtctgaa accaactact cggcagcatt tttaaccggt
     3541 agccaggccg cgtgtaaagc agcctgtaac gcctttaccg atgcagtgct ggaaatcgcg
     3601 cgtaatccaa tccagcgtgc gtaacggagg ttgccgatga tcaatgcact gggattgctg
     3661 gaagtggacg gcatggtcgc cgcgatagat gcagctgatg ccatgctcaa agcagctaac
     3721 gttcgtctgc tcagtcacga agtgcttgac cctggtcgcc taacgctggt ggtggaaggc
     3781 gatctggcgg cgtgtcgtgc ggcgctggac gcaggttgtg ctgccgcgat gcgtaccggg
     3841 cgtgtcatca gccgtaagga gatcggtcgg ccagacgatg acactcagtg gctggttact
     3901 ggctttaacc gccagccgaa gcaacccgta aaggaacccg acgcgccagt tatcgtcgcg
     3961 gaatctgctg acgagttgtt ggcgctgtta acatcagtac gtcagggaat gacggcagga
     4021 gaagtggctg cccactttgg ctggccgctg gaaaaagcca gaaatgcgct cgaacagctc
     4081 ttttctgccg ggacgttacg taaacgcagt agtcgttacc gtctcaagcc ccattaacct
     4141 gtcggaggtg ccgggtgtcc tacaacaccc ggcattaaca tcatgaaaaa gacccgtaca
     4201 gccaatttgc accatcttta tcatgaaccc ttacccgaaa acctgaagct cacgccgaag
     4261 gtcgaagtgg ataatgttca tcaacgacag acaacggacg tctatgaaca tnnnnnnnnn
     4321 nggtgagctg ggatttatcg gttcgcaatg tctgggaagt gcagaaattg cgacgcgccc
     4381 tggtggaacc gagtttgaat taagtacgcc ggacgattac acgatccttg gcgtagtgct
     4441 ttctgaagat gtcatcactc gtcaggctaa ctttttgcat aatccggatc gggtattaca
     4501 tatgctgcgt agccagtcgg cgctggaagt gaaagagcag cataaagccg ctctgtgggg
     4561 ctttgtccaa caggcgctgg cgacgttttg cgaaaacccg gaaaatctcc atcagcctgc
     4621 agtgcgaaaa gtgctggggg ataatttgct catggcgatg ggggcgatgc tggaagaagc
     4681 gcagccgatg atgacggcgg aaagcatcag tcatcagagt taccgtcgat tgctttcccg
     4741 cgcccgtgaa tatgtgctgg aaaacatgtc cgaaccggtg acggtgctgg acttgtgtaa
     4801 tcaactgcat gtcagccgcc gcacgctaca aaacgcgttt cacgccattt tgggcattgg
     4861 tccgaacgcg tggctgaaac gtattcgcct gaacgccgtg cgccgcgaac tcataagtcc
     4921 gtggtcacaa agcacaacgg taaaagacgc cgccatgcag tggggattct ggcatcttgg
     4981 gcaatttgcc acggattatc agcaactgtt tgccgagaag ccgtcactga cgctgcatca
     5041 gcggatgcga gagtgggggt gagggagtta cacccaatcc ctgaccttaa taaactcact
     5101 taaagccgct tccgggctgc catcttttgg ctgataatca tactcccagc gtaccagtgg
     5161 cggcattgac atcaggatcg actcggtgcg cccgccagtt tgcaggccaa acagcgtgcc
     5221 gcgatcccag accagattga actcgacata acgaccgcga cggtagagct gaaaattgcg
     5281 ctcgcgctcg ccgtaggcca tcgcttttcg tcgctctaca attggtaaat aagcgtcggt
     5341 gtagcctttg cctaccgcct gcataaaggc aaaacagtgg tcgaaatctg gcgtgttcag
     5401 atcatcaaag aacagcccgc caatgccgcg ctgttcgtta cgatgtttga ggtagaagta
     5461 ttcgtcgcac cactttttgt aacgcggata aacgtcttcg ccaaatggca ggcacaggtc
     5521 acgggcggtg cggtgccagt gaatggcgtc ttcttcaaa
//
LOCUS       DAWDRC010000228         5541 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6572_length_5541_cov_2.149471, whole genome shotgun sequence.
ACCESSION   DAWDRC010000228 DAWDRC010000000
VERSION     DAWDRC010000228.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6572_length_5541_cov_2.149471"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    239..248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1155..1164
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5272..5281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1418 a         1331 c         1479 g         1283 t
ORIGIN      
        1 ccgtcgtacc aggaagaggt atatcattaa tcagccgcgc cagcccgcta cctaagggac
       61 gcgccagcac cattaatacc agtaaaaacg tggcaatcag taagaaccct tgcgcagcca
      121 tcagaacgcc tccgcattga tcagggcata aaccagataa cccagtaata aaaacaccag
      181 caacacgccg gctatcacgc ctgcactcac agtgcacctc cagtggccta aaagtgatnn
      241 nnnnnnnntg ataccggaag ggtagagttc tggctgcaaa gatttcgcaa aaatctgcgg
      301 gcggggtgta aaaaaagtat aaaaatggca aaagccatga tttaactaat gattagtatt
      361 attttaactt tcatgtaact taattacgcg gtgaatgtaa ataaaccatc aataagcaaa
      421 aataagtggt cggatgagta gtaaaattac acaaacagcg gtactatttt catcagataa
      481 acgaattcat ttttccggtg ctattcaccg ggtagataca aaagggggag aaaagtatgg
      541 aactctacaa agaatatcct gcatggctta tctttttacg ccgtacttgt gcggttgcag
      601 cgggcgttct ggcgctgcct ttcatgcttt tctggaaaga tcgcgcccgc ttttatagct
      661 acctgcaccg cgtctggtcg aaaaccagcg ataaaccggt gtggatggat caggccgaaa
      721 aggcaaccgg tgatttttac tgattgccaa acatgacgtt taaagaaaac cgccggtgga
      781 aacgctggcg gttttctttt ttcggcgtct acacttactc ctgtaaaccg cccaggagta
      841 gtaatgaatc aacaactttt tgatgacagc acccttatcc gcatttttgc tttgcatgag
      901 ttacaccgtc ttaaagagcg aggcttaacg cgcggggcgc ttctcgatta tcacagccgc
      961 tataaactcg tctttctggc gcattcccag ccggaatacc gcaaacttgg cccgtttgtg
     1021 gctgatattc atcggtggca aaatctggat gactattaca accagtaccg ccaacgcgtg
     1081 attgttttgc tttctcaccc tgccaaccgg cgtgatcata ctaacgtttt gatgcacgtt
     1141 cagggttatt ttcgnnnnnn nnnnttttcg cccacatatt gattccacag aacgccagca
     1201 gctggctgcg cttatcgaca gttatcgccg tggcgagcaa ccgcttcttg cgccgctgat
     1261 gcgtatcaaa cactatatgg cgctttatcc tgacgcctgg ctttcagggc agcgttattt
     1321 cgaactttgg ccgcgtgtga ttaacttgcg ccatgcagga gctttatgac tacccatctg
     1381 gtctggtttc gccaggattt acgtctgcac gataatttcg cactggcagc cgcctgccgc
     1441 aattcgtctg cacgtgtgct ggcgttgtat atcgctacac cgcgccagtg ggcagtgcat
     1501 aatatgtcgc cgcgtcaggc tgaatttatc aatgcgcaac tgaatgggct acgaataacg
     1561 ctggcggaaa aaggcattcc tttattgttc cgtgaagtgg atgactttgc tgccagtgtc
     1621 gaaacagtta aacaggtgtg cgcggaaaat cgcattaccc atctgtttta taactatcag
     1681 tatgaggtga atgagcgggc gcgggatgtg gaagttgaaa gagtgctgcg taacgtggtg
     1741 tgtgaaggat ttgatgacag cgtgatcctg ccgccggggg cggtgatgac tggcaatcat
     1801 gagatgtaca aagtctttac gccttttaaa aacgcatggt tgaaacggct gcgggaaggg
     1861 atgccggagt gcgtcgctgc gccaaaagta cgaagtagtg gatcgataga tcccgcgcca
     1921 tccattacgc tgaattatcc tcgtcagtct ttcgatactg cgcatttccc ggtggaagaa
     1981 aaagcggcga ttgcgcaatt acgccagttt tgccagaacg gtgccggaga gtatgagcaa
     2041 caacgagatt ttccggcagt ggaaggcacc agtcgtttgt ccgccagtct ggcaacgggc
     2101 gggttatcgc ctcgccagtg tttgcatcgc ctgttggcgg aacagccgca ggcgctggac
     2161 ggtggggccg gtagtgtctg gcttaatgag ctgatctggc gcgagttcta ccgtcatctg
     2221 atgacgtatt acccttcgtt gtgtaaacat cgtccgttta ttacctggac ggatcgtgtg
     2281 cagtggcaaa gcaatcccgc ccatttaaag gcctggcagg aaggcaaaac gggatacccg
     2341 attgtcgatg ctgccatgcg tcagcttaac agcactggct ggatgcataa ccggctacgg
     2401 atgattacag ccagttttct ggtgaaagat ttattgatcg actggcgcga aggcgagcga
     2461 tatttcatgt cgcagctgat tgatggtgat ttggcagcca ataacggtgg ctggcagtgg
     2521 gccgcttcaa ccggaaccga tgcagcgccg tattttcgta ttttcaatcc gacaacccag
     2581 ggcgagaaat ttgatcgcga aggtgagttt atccgccgat ggttaccgga gctgcgcgat
     2641 gtaccaggga aagcggtgca tgagccgtgg aagtgggcgc agaaagcagg tgtgatgctg
     2701 gattatccgc aaccgatagt tgatcacaaa gaggcaaggc tgcgaacgct ggcagcgtat
     2761 gaagaagcga ggaaaggagc ctgataaaac gcagcgagcg tcttatcagg catggtgctg
     2821 gcttattaag actccagcgc cagcgcgcgg tttctgaatt tcagcgcctg atacagccaa
     2881 atcatcagta ccagtcctac acacgccagt gcgccccagg taatttgatc aaacacttca
     2941 atatatgcat tgatggagta gttgatcgcc ccggaagcat caaacgaaga ttgtgatgtc
     3001 tgatcggcaa tcacgcccgc cagatagttc gcaatcgcgc cggaaagcag catataaatg
     3061 ccggttaata cgccggtcac gccggggatt tcaatgcgcg taatttgcga catagcaacc
     3121 gggtcgataa acagttccgc aaagcccatc accgccaggc ctaacaccat cagtggcaga
     3181 gaagagtgac catacattgc agaccagcgg gcgcttaagg tcagaatgca gaatccggcg
     3241 ctcatcaggc cgagaccaag agcaaatttt ccccagatgc gcacggtacg attacctgcg
     3301 acgctttctt taaccaccca cgccaggaac acaccgcaca gcataactgc gaaggcatta
     3361 atcgactgga acatagcagt cggaacggta taaccaaaca tatcgcggtt aacgaagcgg
     3421 tcgatataaa ggctaatcga gctacccccc tgttgtgcga atgcccagaa caacatgctg
     3481 aagaaggtga gggtaacaat cagccccagc tccttacgct gtttttggtt ttctgctttg
     3541 cgataaattt ttgccagaac cccaaggcca atgatcgtcg cgacaatcaa ggcgtatacc
     3601 gaccactctt tccagaacaa cacggtaatc agcgcaggcg ttgccaccag cagaaccagt
     3661 agccatcccc agttgggcag gagaaagttt gtcgcacgca gtaccttttt gttaacgccg
     3721 cgggtatgtg tgaaatgacg attgccacat aagaaaatga ccagacccgc gatcataccc
     3781 accgccgcca ggccaaagcc catcgcccaa ctttactctt cctgggcgaa accacaggcg
     3841 ataggtgcga taatagaccc cacgttaccc gccgcataca tcagcgagaa accgccatca
     3901 cgacgtggat cggttggctc atacagctcg ccgagcaggc aactgacgtt ggatttaaac
     3961 agaccatagc cgcagacgat aatcgccagg gacagataga ggaatgacgg atggatctca
     4021 ctggcaccca gcaccacatg accgatcgcc atcaacaacg cccccagcat caccgccatg
     4081 cgattgccga gaactttatc cgccaaaaag ccaccgagga ttggcgtgac atataccagc
     4141 gaacagtagg cgctaaataa ctcgtaggcg tgattatcgc tatattttag ttgattggtg
     4201 agatagagga tcagcagggc acgcatgccg taaaagctaa aatattccca gatttgcagc
     4261 gcaacgacat agtaaatagc gcgcggttgt gatgcgtgtt tattcataat aattccacaa
     4321 ttgactgcaa cgtcacgtaa tagttgcgcg gcgtgcttgt gttttcttaa agttaaaact
     4381 ttgatacaga tctgattatt tatgcaatat gctgtctgat tgcataaata tacattagct
     4441 gaagcgtgat gatataaaca atttgcgtct tttggcgtcg ggtttgtttc ttaatatgtc
     4501 aaaagattgg ctatcgacga acaggcgagg taacgctatg ttaacggtgt agctatcaga
     4561 atacggaagg agcgaagatg aaaaacaccg aactggaaca actgattaac gaaaaactga
     4621 acagcgcggc gattagtgat tacgcgccga atggtttgca ggtggaaggc aaagagacgg
     4681 tgcaaaaaat tgttaccggt gtcactgcca gccaggcttt gctcgatgag gcagtgcgtc
     4741 tgggcgcgga tgcggtcatt gtgcatcatg gctacttctg gaaaggcgaa tctccggtca
     4801 ttcgcggaat gaagcgtaac cgcttaaaaa cattgttggc gaatgatatc aacctgtatg
     4861 gctggcattt gccgcttgac gcacatcctg agctgggcaa taacgcacag ctggcggcgt
     4921 tactggggat cacggtcatg ggcgaaattg agccgttggt gccgtggggc gaactgacca
     4981 tgcctgtgcc gggactggaa ctggcctcct ggattgaagc gcgtctggga cgtaagccgt
     5041 tatggtgtgg cgataccgga cctgaggtgg tccagcgcgt cgcctggtgc acgggcggcg
     5101 ggcaaagttt tatcgatagc gccgcgcgtt ttggcgtgga tgcttttatt actggcgaag
     5161 tttctgaaca gaccattcat tcagcccgcg agcagggatt gcatttttat gctgcgagtc
     5221 accatgccac tgaacgcggt ggtattcgcg cattgagcga gtggctgaat gnnnnnnnnn
     5281 naagtgcaag gggcgcgttg ttttctgata ggggaaacgg tggtagtact ggaactggaa
     5341 ccgccggtga cgctggctag ccagaaacgg atctggcgac tggcacagcg tctggtggat
     5401 atgccgaatg tggttgaagc cattcctggc atgaacaata tcacagtgat tttgcgtaat
     5461 cctgagtcgc tggcgctgga tgccatagag cgtttgcaac gctggtggga ggagagtgag
     5521 gcgctggagc cggagtctcg c
//
LOCUS       DAWDRC010000229         5510 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6617_length_5510_cov_2.591751, whole genome shotgun sequence.
ACCESSION   DAWDRC010000229 DAWDRC010000000
VERSION     DAWDRC010000229.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6617_length_5510_cov_2.591751"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2167..2176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1286 a         1370 c         1531 g         1313 t
ORIGIN      
        1 cgccggggct ggattcgaag tgagcactta cccctggtat gacgaagcga ctaacggcgt
       61 gcgctttaat gacctgttgg cgacgctgaa aacattacct gcccgcagta ttgtgttgct
      121 gcatccatgt tgccacaacc caacgggtgc cgatctcact aatgaccagt gggattccgt
      181 gattgaaatt ctcaaagccc gcgagcttat tccattcctc gatattgcct atcaaggatt
      241 tggtgccggt atggaagagg atgcctacgc cattcgcgcc attgccagcg ctggattacc
      301 cgctctggtg agcaattcgt tctcgaaaat tttctccctt tacggcgagc gcgtcggcgg
      361 actttctgtt ctgtgtgaag atgccgaagc cgcaggccgc gtactggggc aattgaaagc
      421 aaccgttcgc cgcaactact ccagcccgcc gaattttggt gcgcaggtgg tggctgcggt
      481 gctgaatgac gaggcattga aagccagctg gctggcggaa gtagaagaga tgcgaactcg
      541 cattctggca atgcgtcagg aactggtgaa ggtattgagc acagagatgc cagagcgcaa
      601 tttcgattat ctgcttaatc agcgcggtat gttcagttat accggtttaa gaaccgctca
      661 ggttgaccga ctacgtgaag aatttggtgt ctatctcatc gccagcggtc gcatgtgtgt
      721 cgccgggctt aacgcgcaaa atgtgcatcg cgtggcaaag gcgtttgctg cggtgatgtg
      781 attttaatca acaggcggat ctctccgcct gttcctgact ccagccatcc cctactattc
      841 tgtccctttt ttcgcccact tttacatcat ctcttttcta taagtttttg aaaattagtg
      901 tttttaactg ttggtacgga ttgtgcagta atgcggctgt ctatgaccat caggagaaaa
      961 acatggagaa catgacctcg cccggcacgc tgcttagtgg agataacgcg acctggcttg
     1021 aagagtacta ccagacctgg ttgcgtacgc cggaacaact cccggaagac tggcggcgtt
     1081 tttttctttc acctgaactg actgtccaaa gcgtatccgg tgataacaat atcagcggtg
     1141 cgacgttaaa aaaacaggct gcggtaatac aactgatcaa tgcctggcgc acgcaggggc
     1201 atctgcgcgc aaagcttgat ccacttggtt tgaatccccc ggctgacgtt ccctccctcc
     1261 agcctggttt ttggggatta agcgaagaag acttgttgca ggagtttagc gtcacctttg
     1321 gcgcacacac cacacaaatg ccacttaagc aactgcttaa cctgctagag caagcctggg
     1381 cgggtagcca ggcttatgaa cttgctcatc tggaaaatcg tgaagaaatc aactggctgt
     1441 tatcacggat tgagagcagc aatgcgccac aagctgatgc ccaaacctgc attgcccgtt
     1501 ttgaaaaact gatggcggca gagacgttag agcgttatct gcatacccgt tatgttgggc
     1561 agaaacggtt ttctctggaa gggggcgaaa gtgcaatccc ggcgctcgat acgctgacaa
     1621 aatgcctgcg tgcgcaaggt gtggaggaaa tggtcattgg catggcgcac cggggaagac
     1681 ttaatgttct ggttaatctg ttgaataaag acccggcaca gctttttgct gagtttgaag
     1741 gcaagcagac gatcggcagt ggttcaggcg atgtgaaata ccacatgggg tattccagca
     1801 atctggaaac acctgccggt tcgctgcatg tggcgctggc gtataacccc tcacatctgg
     1861 agatcgtgaa tccggtggtg ctgggccagg tgcgagccag gcaggaacgc cgtggtgagg
     1921 atggtcaggc aaaagtggtg ggcgtactta ttcatgggga ttccgcgttg ggcggcctcg
     1981 gcgtcaacca aaccacgttt aacttatcgc agactcaggg ctacggcact ggcggtacgc
     2041 tacatctggt gattaacaat cagattggct tcaccacttc ccgcttgcag gatatgcgtt
     2101 cttcacggta ctgcaccgat attgccaaaa tggtagcggc cccaattatc cacgttaatg
     2161 gcgatgnnnn nnnnnngttt gccaggtaat ggaactggcg tgcgaatggc gagacacctt
     2221 ccggcgcgac atcatcatcg atatttgctg cttccgtaag cacggacaca acgaaagcga
     2281 tgagccacgt cttacccagc cacaaatgta tcaggcagtg gatgctcacc ctggtacgct
     2341 cgcccgttat ggtgaatctc ttgcccgccg cggcctgtta actcaggcgc aacaagatga
     2401 gatgacggca cgctatcgcg actggctgga tagctgccag aaacgtgaac cgcagcctct
     2461 caaacctgca attcactctt ttagcgctaa ctggtatggg ttgaccaacc ctcactggag
     2521 cgccccggta tctacggcat tgcctcggca aaagctggca gcgtatggtg aaatcatttc
     2581 tacactgcca cccgatgtgg tcgcacatcc taccattaaa cggcaactga cgttacgcca
     2641 ggatatggcc gcaggtactc agccaataga ctggggaatg gctgaaatgc tggcctatgc
     2701 atcgcttgtg gatgcgggtg ttggtgtgcg tctttctggt gaagactcag ggcgtggcac
     2761 gttcagtcat cgccatgcag tggtacatca tcaaactgaa gcccgtcgtt atctgccgtt
     2821 acagcatatc cgcgctggtc aggcatcttt cgatgtttat gattcggtgc ttaatgaaga
     2881 agcgctctta gcctttgaat atggctattc aacgtctgcg cctcagcaac tggttatttg
     2941 ggaagcacag tttggtgact ttgccaacgg cgctcaggtt gctattgacc aatttatctc
     3001 gtctggcgaa accaagtggg atcgctatag cggtttaacc attcttctgc cgcatggcta
     3061 tgacggacaa gggccggagc actcttctgc gcgtcctgag cgctggctgc aactgtgcgc
     3121 agaaaacaac atgcaggtgg tgatgcccag tgagtcagca caaatgttcc atttgctgcg
     3181 cggtcaggca ttacgcccaa tgcgcaaacc gttagtgata atgatgagta agcgtttact
     3241 tcggtttaaa ggcgctatga gcgagctttc tgaattcact gatggcgctt ataaaccggt
     3301 agtcaccgat ccgcaattgc atcagtccca gaaagttaag cgcgttattt tgtgtagcgg
     3361 ccaggtctat tacgacgtac tggaagcccg taagcagcgt gagtgtgaag atgaggtagc
     3421 gattgttcgc ctagagcaac tctacccgtt cccggtcgca gaactgaatg atgttctggc
     3481 gagttggccg aactgctgcg aatggatttg gttgcaggaa gagccagaaa accagggcgc
     3541 gtggcggcag attcgtcatg agctggcggc actgaaaatt aacactcctt actggcagta
     3601 tgcaggccgc ccggcagcgg cagcaccagc gacagggtat gggcgtgtac ataaacaaca
     3661 aatcgacgaa tttcttgctg ctgcgtttgc agatattcag ccatgaaagg cgcacaaaaa
     3721 taataaggag aacattttat gatcgaaatt actgtccctg tattaccgga atctgtcacg
     3781 gaaggcacgc tgacaacctg gtgtaaacaa gaaggcgagc acgtaaaacg cgatgatgtg
     3841 attgccgagc tggaaaccga taaagtcatt ctggaaatac cggccccgca cgatggcgtg
     3901 ttaagcaata ttatcgtcag cgaaggtagc acggtcacgt ccgcgcaact gttggcgcat
     3961 cttaagccgc aagctgtcat agaagaaacc gtcacaccag tcaccgaaac cctggcgatg
     4021 ccttccgcac ggctggaggc acagcgtagt ggtgtcgaac ttgctgacgt tgcggggagc
     4081 gggagaaatg gacgtatcct gaaagaggat gtacagcgcg ttactcctgc accagtcatt
     4141 cagcctgaac gggtggcgga gatcgcgcca gctaaaccct tgacaccggg tgctcgtcag
     4201 gaacgtcgtg agccgatgtc gcgcttacgt cagcggattg ctgagcgtct gctggcttcc
     4261 caacaaaata acgccattct taccacattc aacgaagtaa acatgcagag cgtgatggat
     4321 ttacgcgccc gctggaaaga tcgttttgcc gagaagcacg gcgtgaagct gggctttatg
     4381 tccttctttg ttaaggccgt tacccgggcg ctggagcgct tccctgtagt gaatgccagc
     4441 gttgatggca acgagattat ctggcgtgat tattgcgata tcggcattgc agtgagcagc
     4501 aaccgcggcc tggtagtgcc tgtgttacgc aatgcgcaat cactctcttt ggtggaaatt
     4561 gaacggcaaa ttgccgaata cgccacacag gcacgcaatg gcaaattgcc gctggaggcg
     4621 ttacagggcg gcacgttctc tatcaccaac ggcggtactt ttggttcgat gatgtcaacg
     4681 ccaatcatta acccgccgca gtcggccatt cttgggatgc atgccatcac gccgcgcccg
     4741 gtcgcagaaa acggccaggt tgttattcgt cccatgatgt accttgctct cagctatgac
     4801 catcgcatta tcgatggtca ggaagccgtg cagacattgg tggcaatacg tgagctactg
     4861 gaatcaccgg aacaactgtt gctggatctt taaggagggc gccgatgagt acaatttttg
     4921 atgtggcagt tatggggggt gggccaggtg gttacgtagc ggcactgcgg gctgcgcaga
     4981 atgggctttc cgtagtatgt attgatgatg gtgttaatgc acagggcgaa ccttcgccgg
     5041 gcggtacgtg tctcaatgtc ggctgtattc cgtcaaaatc gttgctgcaa tcttccgaac
     5101 tgtacgcgca agtgcagcat gaggcgagca ttcatggcgt taatgttgac ggcgtatctt
     5161 tcaatgcagc tgcgatgatt caacgtaaag acgctatagt aagccgcctg acaatgggca
     5221 tcggtttgct gtttaagaaa aataaagtga agcacctgtg cggcctcgca acgttagaac
     5281 atgctcagga tgagatttgg caattacgtg taaacgatca acaaatccgc gcccgtaatg
     5341 tggtgattgc aacaggttct cagccgcgtc aactgcctgg cgttaccatt gataatcagc
     5401 aaattctcga taatcgcggc gccctggcgt taaccgctgt gccgccgcgt ctcggtgtga
     5461 tcggcgctgg cgtgattggc cttgaactgg ggtctgtgtg gaatcgggtc
//
LOCUS       DAWDRC010000230         5503 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6626_length_5503_cov_2.312225, whole genome shotgun sequence.
ACCESSION   DAWDRC010000230 DAWDRC010000000
VERSION     DAWDRC010000230.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6626_length_5503_cov_2.312225"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4381..4390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1405 a         1532 c         1353 g         1203 t
ORIGIN      
        1 ccggaatcgc tgaaggtcga caacaaaggc ttacgcgatc acatcgaact ggcgacccgc
       61 tggctgtggc gcaagctgga cgaaggcaaa acgttaccgc tgaaaccgct gccgctaaaa
      121 gtggtttatc acactccgtg ccatatggaa aaaatgggct ggacgctcta caccctggag
      181 ctgttgcgta aaatcccggg gcttgagtta acggtgctgg attcccagtg ctgcggtatt
      241 gcgggaactt acggtttcaa aaaagagaac taccccacct cacaagccat cggcgcacca
      301 ctgttccgcc agatagaaga aagcggcgca gatctggtgg tcaccgactg cgaaacctgt
      361 aaatggcaga ttgagatgtc gacaagcctt cgctgcgaac atccgattac gctgctggcc
      421 caggcgctgg cttaaactcc tttctgatgc ccggtaagca tgtggttacc gggcattttt
      481 gcgtacacga ttccgcgctc aatgtatacg ttgcaacgca gtgaaaattc ctctgaaaac
      541 gtctcgcaaa ggctgaaact ggcagatgtc aaaggcctgg gataaccgta atgtcgcgtc
      601 atcataaata tcaggtgacg gacaaccatg accgaatcaa caacctcctc cccgcatgat
      661 gcggtgttta aaacctttat gttcacaccc gaaaccgcac gggattttct ggaaatacat
      721 ctaccagagc cgctgcgcaa gctttgcaac ctgcaaacct tacgcctgga acccaccagt
      781 tttattgaaa aaagtttacg cgcttactac tcggatgttt tgtggtcggt ggaaaccagc
      841 gacggtgacg gctatatcta ctgcgtgatt gaacatcaaa gttctgcaga aaagaatatg
      901 gcttttcgac taatgcgcta tgccaccgcc gccatgcagc gccatctgga caaaggctat
      961 gacagagtcc cgctggtggt gccgttgctg ttttatcatg gcgaaacctc gccctaccca
     1021 tactcactta actggctgga tgagtttgat gatccgcaac ttgcccggca gttgtacacc
     1081 gaagcttttc cgttggtgga tatcaccatc gtacctgacg atgagatcat gcaacatcgg
     1141 cgtatagctc tgctggaact gattcaaaag catattcgcg accgcgattt aatcggtatg
     1201 gtcgacagga tcaccacgct tttggttaga gacttcacta atgacagcca gctacaaaca
     1261 ctgtttaatt atctgctgca atgcggcgat acctcccgtt tcacccgttt tattcaggag
     1321 attgccgaac gttcaccact acaaaaggag agattaatga ctattgctga acggctacgg
     1381 caggaaggac atcaaattgg ctggcaagaa ggtaagattg aaggctggca agaaggtaaa
     1441 ttagaaggtt tgcaggaagg catgcatgaa caagccatta aaattgcttt gcgcatgctg
     1501 gagcagggca ttgatcgtga ccaggtgctc gcggccactc agctaagcga agccgatctg
     1561 gcagcgaata accactaatt aacacaggcc cacagccgat ccccatgggc ctttgatatc
     1621 aataactacc ttttacgcgt ggcccatttt tgcctgattt aaacatcgcc agtcgttgat
     1681 ccagggcatc gctgtagagc atcgtgtcaa cgccaacagc gacaaagttc gctccccacg
     1741 ccaggcattg ctgcgccata tcaggagcca cagccagaaa accagccgct ttacccgcag
     1801 cacggatccg ccgaatgctg gtttcaataa ttcgctgcac ttccgggtgc ccggcgttat
     1861 ccgggtagcc caacgacgca gaaagatccg caggtccaat aaacacgcca tcaatccctt
     1921 cgacatcgag gatttcgtcc agattatcca gtgccgtttt actttccacc tgcaccaaca
     1981 gacaaagcga atcgttgact tgcgccatgt aattctcaat gcgtccccag cgtgcagccc
     2041 gtgccacact ggccccaaca ccacgctcac cgtagggagg atagcgcgtg gcagacacca
     2101 cctgacgtgc ctgatcggca gtatcgacca tcgggatcag tagagtttgc gcgccaatat
     2161 ccaggacttg tttaatcagc ggcttactgc cttccaccgg acggatcacg gggtggctgg
     2221 catagggcgc taccgcctgt agctggtgat aaagatcctg aatggtgttt ggcgcatgct
     2281 ccccgtcaat cagcaaccag tcataaccgg aagtggcggc aatttctgcc atataggcag
     2341 tcgttgagct taaccacaga ccaatttgca cttcgccctt gcgtaaacgt tctttaaagg
     2401 gattggttaa taatgcgttc atcgtgttcc ttaaactatt taatgatgtg ccacgtcggt
     2461 ctgcgcaaca gtgcgattta cacgcagagt gaaaataatc agcgaaccga tgaccgccac
     2521 cgctgccagc gtcagtaatc ccgccgcatc gctggaaaat aacgtttctg ctttcacgcg
     2581 caggatcggg gcaataaacc cgcccactgc accaaacagg tttataaaac caatccccgc
     2641 agccagcgcc gtaccggaaa gaagctgtgt cggcatcgtc cagaacaccg gttgcacggc
     2701 aataaacccg atggctgcaa cacacagtgc tacgatcgcc agtactggag aaagcagccc
     2761 ggacagacca ataccaatgc ccgccgccag taatgtcagc gctgcgacat tacgccgttc
     2821 gccggtttta tcggagtagc gcggaataag ccaggtccca aacaaggccg caacccacgg
     2881 aatggcggtg accaccgacg ccgtaaaacc cacttttgtt cccagcaatg ccgcaacctg
     2941 ggtcggcagg aagaaaatta atccgtaaac cgctacctga atggtcaggt agataatcgc
     3001 cagttgccag actcggccat tacgcagcgc atcgctcaat cgagaagtca ctttctgttg
     3061 ttcttcactt gccagttgat tgataagcaa cgttttttct tgtttactca gaaaacgtgc
     3121 ctgctccggt gtgtcatcaa gccaaaagaa tgtgaatacc ccagcgccga ctgccaacaa
     3181 tccttcaata acaaacatcc agaaccagcc gggatgcccc ataaatccat gcatctccag
     3241 cagcgcgccg gaaagcggtg atcccagtgt taacgccagc ggtgcgccca tatagaacag
     3301 ccccataatg ctggcgcgat tacgctgcgg aaaccattgc gaggtgagat aaatcatacc
     3361 agggaaaaac ccggcctccg cagcaccaag cagagtgcga acaatcagaa atttcgcttc
     3421 agtatcggcc catgccatgg cagccgaaag aaatccccac agcagtgttg tcgtaccaat
     3481 ccaggttctg gcccccagtt tgcgcatcaa aagattcgcc ggaaccccca gaaacgcgta
     3541 taccacaaag aaaatgcctg ctcccagcgc ataagcttca ttactcagcc cggtatcaat
     3601 ctggtaggtc tgtttggcaa aaccaatatt cgaacggtcg agaaacgcca gcacatacag
     3661 cgccaacata aacggaatta aacgcgcgcg gtttttcttc accacggcgt caagcaaagc
     3721 ggtgctcata aaaagctcct tagaatatgt gggcgttacc gttgtcacag caacgcccgg
     3781 agtgattagt ggctgtaggg gcgtttcaaa ttgcagtcac gattgagttc gacgccgaaa
     3841 ccgggtttat caagcaccga tttatgaata cgaccattca ccggaaccgg ctcattaagc
     3901 agaatcggat caaattgcgg acgcatcgtt gaacaatccg ggctggtcat caggaattcg
     3961 ctgaatggcg tattggtgaa ggtgatcacc gcatggtggg agtaaacaga cgaaccgtga
     4021 ggcaccacca gttgccccct ggatttggcg attgcggcaa tttccaccag cgtggttaag
     4081 ccaccgcacc agccaacatc cggctgcatg atgtcgatac cagtttctga aagcgtacga
     4141 aaagattgca gtgtgccgtg gtgctcacca ccagcggcgc cctcaaggcc atgatgctcc
     4201 tgctggccct gtgcgccgcg ggggcggtct cggcccggat ctttctggtg aacaatgaga
     4261 agaaaaagca ggacggggag gaggagcatc cgtgaagcag ctgcgtgtca tgaccatctt
     4321 cggcacccgg ccagaggcca tcaagatggc ccccctggtg caggagctct cccgccgccc
     4381 nnnnnnnnnn ggcgcgcaag cgtgggccag tttggtggca tagttcacgt cctgactcat
     4441 ccagcagtcg agcattaacc agaaatcctc accgcatttt tcacgcatat ccgcgaccat
     4501 agcggcatct ttgcggatcc ccgcatcgcc atcatgtggc ccccagtgcg tcggcatttt
     4561 gccaccgata aagcccatct cttttgccag atccggacgc gcacctgtgg cgtagaactg
     4621 aatctcatca cgaactgcgc cgcctaaaag tttataaacc ggaagcccga tcactttgcc
     4681 gaacagatcc cacagagcca gatcgacaca ggaaatcgtg ttcatcacca ggccaccaga
     4741 gccggagtaa tacagggtgg cattgagcat ttgatcgtgg atcagtttga tatcactgac
     4801 acatttaccc tcaatgaaac ggttaagatg tttttcgaca ataaaacagc ccatttcacc
     4861 ggctgtcgaa acagcgaatc cggtctggcc gttttctgct tcaacttcaa caatcaacgt
     4921 gccaagaacg ttaatgccaa atgactggcg tgactgctcg taatcgcggt atttactcat
     4981 tggggtggca atatgatcgt ctatccagtg attcgccccc tggtcgtgat aatcaccgcc
     5041 gccagcgcct ttttctgctg tcgcaccgcc agtaaaccag gcgcgaacct gtttgatttt
     5101 tggtagggtc atgatgttct ccattgttat gaggcctgta agtcaaaggg acttttccat
     5161 cccaacaaac gtgaaatatc cctggcgcag gcaatggcct tgcccgccag ataatcacgg
     5221 tattcttcat tgatttgtaa gcgggtaccg accaccgaaa tcgcagcggt aagctcgtta
     5281 ttggcgttaa acaccggcgc agcgacacaa cggacatcgg cataatcttc gccgttgtca
     5341 tagctccagc cctgacggcg aatacgcgcc agctcctcgt agagccgttg tggatgggta
     5401 atggtagtgg gtgtcatctg ctcccagacc agctcttcga taatactttt ctgtacagct
     5461 tcaggttgcc aggcaagtag gcatttacct attccggagc gat
//
LOCUS       DAWDRC010000231         5485 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6648_length_5485_cov_2.488766, whole genome shotgun sequence.
ACCESSION   DAWDRC010000231 DAWDRC010000000
VERSION     DAWDRC010000231.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6648_length_5485_cov_2.488766"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3395..3404
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1449 a         1334 c         1461 g         1231 t
ORIGIN      
        1 gaggccactc tgacgctgac ccagccaggc ggcgtggggg tcaaaactca catcaccgcg
       61 agaatgcgca gtgattttac tgcctcggat gagaaagacg ttatttttac ggtgatcacc
      121 agccccgata ccgacaaagc gcggatgtgg ggccatatgt tgggcattat tgaagcgaac
      181 aatatcttta aacgcccacg gctggcggat gaaaccgaca acgaactggg atcggtgcgg
      241 gaaaataacg aagactgggc gctgtttgat cagaatagca gtatgcaggc tgagtgtggt
      301 ttaggtcata ttccgagcca aagttcattg cacagtttgt ttgccgctca tccagccaat
      361 gccatcggca ccgaatatgg ctggccaacg ttgcaaaaag cgtatcttag tgcggtggaa
      421 gagacgtcgc acgcgtcggt aaacctggcc acgggcaaca tcgacaccta ttccggcttt
      481 aagcaaaact atctctcttg ttcgggcaat gagatggtcg ccaagattgc cgcgaccacc
      541 gatcgtgatg tttccgcagg cagcagagcg caggccaaag tgggtgacac gatcaccatg
      601 acggtacgca cctttaatgc cttgaataat gcacccgtgc cgtataccgc gttcaccatc
      661 actaaagata tggggaaaaa tcgtcagggg cagactacag gttttgatga tccaacccgc
      721 ggcgcaatcg agatgaatgg cacactctac ggaacatcgc aaccgtcttt agtctatgcc
      781 ggtactacgg atgcgcaggg ttttgcgact gtggaaatta agcaatcgca aggtgtgggc
      841 ttatcgacgc cgctaaatat tgtgcctgtg aactcctata ttcccaatac tgttaattac
      901 aatgtgattt tcactacgct caccagtccg gatgcggttg gcgcgcagat gtgggggcat
      961 atggatgaga cgattaccgt cgatgcttta acctttgccc gcccccgact ggctgcggaa
     1021 gtgttcagtc cggacggcac attaacggaa aataacgaag tctggtcgcg agtcagccag
     1081 gccaacgcct ccagcaccag taaaggtggc tgtggcgcca atatgctccc acgccgttct
     1141 cagcttagcg cgctctatga tgccaataac ggtgacggtg tacagacagt acacggctgg
     1201 ccaacgcagc gtcagcccta ctggagcagt tcgcccgcag atcaggtacc gcattattac
     1261 accatagccc tcaacgatgg ggcgcggacc gttggcggta gcacggcggt ttatgtcagt
     1321 tgcctgacga cggctaacaa tcctgccagt agcattacgc tggaagtggt ggatccggcg
     1381 cagtggaacg cggctgcaaa tgcggcgaag ctgaaaaaag gcgaaacgct acaggtaaag
     1441 gtgacggtaa aagatgcgca gggcaatcca ttgggtgata taccctttac cctgaaacgt
     1501 ggcgatggtt atacccgctc agaggaaaaa cacgttgctg gtagcagtga tgcgctggtt
     1561 gcgccggttg tggtcaatgg tggcctggcg gacgaaactt cgcttaataa taccgctgca
     1621 gcatacagcg ccatgaccgg cagcgacgga actaaaattc tcactattac ccgcccggat
     1681 acccatggca cgaaaacgtc gctgaccgct aggctctatt cagatacaac gaaaaaagcg
     1741 accctagata ccatttttac cgttgtaacc agtccggaca gcgataaggc gaaaatgtgg
     1801 gggcatatgc ctgaaacagt gacggctgca gatggagcgg tctttaaacg cccattattg
     1861 ttgaaagagt tatccagtac gtcggggcga acagcgattg cagaagagaa tgaagactgg
     1921 gcgcaattta cccaaaccca ggctataagc acatcctcta atggttgtgg tagtgaatat
     1981 gttccctccc aggccgggct ggagtcattg tatgaagcca accgcggcaa tgcgatgaaa
     2041 acggttcagg gctggcccgt cgcctcaagt tacctttcca gcacgacagg tagtagtagc
     2101 ctggaacagc gtgacttcaa ggccgtcaat ctcagttcag gcaccagttc gataattcca
     2161 tctgccacaa aagaactgct tacctgccag acgacgccga ttgttaaggc aagccaaatt
     2221 gttcttgaag ctgcagaccc cacaaaattt gaccgtatga ataatgttgt caaagcgaaa
     2281 aaaggtgaag aagccgtatt acgagtgacc actaaagatg cgcagggtaa ccctgtggga
     2341 aataccgcat ttaccctcaa acgaaacacc agcgtgaaca gggccaatgt gtcaactacg
     2401 acgtcgattg cttcgctggc ggtaacggat gcctggggaa atactcaaaa tgattttctt
     2461 tcaacgaccc tggttattta tggcgtgacg ggggctgatg gcaccaccac tttcacattg
     2521 aagcaagacc agactaccgg gctaaaaaca gaactcacgg cagcgttgga ttcctccagc
     2581 agcactaaat cgacgcttcc cgttgtcttt acggtgctta ccagcccgga ttcgccaaaa
     2641 gccaaattct gggggcatat ggctgaaacg gcgacaggcg atgacggtct tatttatcga
     2701 aggcctttgc tgagggacga gaatagtgct accaccagta tcggtaccct ggtggaagag
     2761 ggggaggcct ggtctacgtt cccgtcgggc caggctaacg atacctcgat aaatggttgt
     2821 ggcgcggagt atgttccaac tgacaatgaa cttagggcca tttatgcgca ccaggggagt
     2881 agcgcattac atgatgctat tggctggcca gtgtcgaggt tctatatctc gaataccgtt
     2941 gcagatacgt tcactcaaac ctttacgtat gatgtcgttt cgctgaagac aggagatgaa
     3001 acgcagatgc cctcatctgg tggtgcatta ctgagctgcc ggaccacgcc tgtcgccgtt
     3061 gccagtcaga tcattgttga ggcgaatgat acggcgcaat ttgtcaaagt tgacgacacc
     3121 ttgagtgcgt tgaaagtgaa aaaaggcgaa gacgcggtga ttcgcgtggt gactaaaaat
     3181 gcgcagggaa atagcgtgcc caatgtcccc tttattctca gacgtgaagg ctcgaagaac
     3241 cgtcaaaatg ccgagatgat aaataagtcg attacggtaa ttaacgcggc aggcgcttct
     3301 gcgaggatga atagttcttc aagcctgctt tatggggtga ctggcgcgga cgggactacc
     3361 tcctttacgg taaaacaaga tgattctatg ggccnnnnnn nnnnatcagc tgacgataga
     3421 atccaacaaa ttaccggtca tgtttaccgt cataaccagc ccggatacgc cactggcaag
     3481 ttactggggg catatgcctg aaacgtttac tacccggtcg ggaattgcct ttaagcggcc
     3541 attgcttact gccgagcatc cagcggggca gtcaaccatg gctaataatg aatcatggct
     3601 ttctttgaat acggcagcaa aaaatgatgt cagcaaatca gactgcggcg aaccttatca
     3661 accgttatta agcgaatttc aagagttata cagtgaacat cctaatggtg cgatagggac
     3721 cgatttaggc cttccgctga ctaacacctg gtgggcttac gataaaattg cctacgcgaa
     3781 cgtctggtac gatcagtcta ttaatttatc gaatggatca agctccaggg cattatcaaa
     3841 caccgtggca tttgtgtcct gtctggtaaa ccctcatgca gtggcagctt ctataaagat
     3901 gacctccacg gcactggatg cggagaaaac ggccagcaac gatggtcgcc ctagtgcaac
     3961 ggcggcgaaa ggtacggcga tccggatgac ggtgattgtg cgggatagcg gaggaaatct
     4021 gttgccaggc gcaaacttta atctcatacg cgggactgcg ttggatcgtg caaaaaacag
     4081 gctagattct acgtatgatg atcttactat cgtgccggtc acccctgccg gagtaaacat
     4141 gtcactctat aataatgggg cacaggcact tctgacgaca ggaagtgatg gtaaagcgac
     4201 ttttgatgtg acgcaaaacg agacttatgg gctggcaact ccgctcactg caacattgat
     4261 gcgcgacacc acaaaaagtg ccacgatgga tgttattttc actgtgataa ccagtccgaa
     4321 ctcaccgaaa gcaaaatact ggggtcatat gcccgacacg tttaccagta gggcgggggt
     4381 gacctttaaa cgaccgctac tggcagcaga agcgaccttg gggtcctcgg taagtaataa
     4441 taatgagtcg tggtcatacc ttttttatac caataaagtt acgccggatt gcccggttga
     4501 gtatcagccg cgtctcaatg agttacaggg gctatataat gatcatccag gcgggacaat
     4561 actgacagat ttggggttgc ctataactgc cggaagtgga aattggtgga cttatgaaat
     4621 gagcaccact gacgcgttga cgtggtatta cggcgtcatt aatttgaaaa cgggacaatc
     4681 gacgacgact attaatggat acgctttaat gctctgtctg acgcagccac attcggcacc
     4741 agcaagcctt acgctgtcat cgactatgat gagggccgaa ctgccagcaa tggtggtacc
     4801 cccacgtcat ctgtgaaaaa aggtgagatg cttcccatcg tggtgacgat caaagatgcc
     4861 aatggtaatc ctgttggcgg cgaaggtgtt acgctcaagc gcgtgcaggc gaaaagccgt
     4921 tcagggatct ctgtatcctc caataccgta gatgatctca ttttagatga agtcaccccg
     4981 acatcggcgc gtatctcctt caaccagaat acgtcggcat ggagcggctt caccggcagt
     5041 gatggcacga taacgttcaa cgttacccaa aataacaccg ttggtctggt aacgccgttt
     5101 acagcttcat tggcgcgaaa tccgcaggtt acggctaatc aggacctgat attcacggta
     5161 gtgaccagcc cggattctgc gaaggctaac tactgggggc atatgccagc aaccttgacg
     5221 gcagtgaatg gtgcggtatt tgaacgcccg aaattgtgga gtgaactgac atcgacttct
     5281 ggcgtcggca agatcaataa caataatgaa gactggccgt actttacccc aacacagaaa
     5341 tcagatgcga gtgtgagccc ttgtgaagta gcaaggcagc cactattcaa tgatttaagt
     5401 tcgttgtctg cgcgttatcc gaataacacg tttgtcactg aaactggctg gcctgcgtat
     5461 tacacctggt gggcagagga taaat
//
LOCUS       DAWDRC010000232         5473 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6663_length_5473_cov_2.116094, whole genome shotgun sequence.
ACCESSION   DAWDRC010000232 DAWDRC010000000
VERSION     DAWDRC010000232.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6663_length_5473_cov_2.116094"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1374 a         1411 c         1367 g         1321 t
ORIGIN      
        1 gacgtttatc gcgccataag cgcagagtaa accaggtcac cagcgcggca gttaacaggg
       61 tcgcaaagat aacgcctgcc cgcacaccaa agttattcgc gccaaatagc cattgcccaa
      121 tgctgttaat ccagtatccg gcaatgggtt tttcgaaata acgtagccct aacagatggg
      181 gaacaatcca gtcgccggat gccagcattt cccgactgat ttccgcataa cgcgtttcat
      241 ctggttgcca gagcagacgc gtgctgatcg gtaacaggta atagcaggca ataaatgcga
      301 agaggccgat aaggtaacgt accgatttca tcagtgacta cccgcaattt gttggcaacc
      361 cagccagcct tcacgtccgg caatatttcc gcgaacaact tgtccgagcg gcaacgtccc
      421 tgataacagt tcgcttaaag ggcaaaatgt cacgccttcc tgagctgcgc gtttcaggag
      481 atccacaaaa ttatgctgat aagcgcagcc ttcaacttcg gcatgaatgg tataaaccgg
      541 cgtgccttta tctcgctgga tgcggttgag taaccaaccg ttaaaatctt ctgctttcac
      601 atcccgacca atcacttcat cccaggtggg taaggtcacc ggaatttgcg cagtgccagg
      661 ggttcctgat tcgagcaatg gacgaaacgg catcgcccca cgacaatcgc tgttgtagcg
      721 caaatggaac gcttcttttg cttcgatcac ctgttggtca gcacgccagc cagcagcggc
      781 agaacaggtt accggttgac cgataatctc ttccagggtg cgaaggccgc gtgcaatatc
      841 gtcgatcatt gtttgccgat cccaattacc gctatgggct tgccaggcat ggtgatccca
      901 ggcgtgcagg ccgacttcgt gatgtttagc cgcttcacga atgatatcgg cattggcatg
      961 accaatctct ttacctggcc aggccgtacc tgccagtaaa atatcccagc cataaagcga
     1021 tgccgcgttt gagcgcagca tcttccacaa aaactgtggc ttcaccagtc gccagagatg
     1081 gcggcccata ttgtccgggc cgacgctgaa aaaaatgctg gcctgaatat tatgcttact
     1141 caagatttcc agcagacgcg gcacgccttc acgggtgcca cgaaaggtat cgacatcaat
     1201 gcgtaagccg actttggtca tgatggttta tccgtaagat caacggtgcg caggaagaaa
     1261 tccagcgttt cgtcgatggt ttcctgcata tcaattttcg gttcccagtt caggcagcgg
     1321 cgggcattgc ggatgctcgg tttacgatgc tctacgtcct gatatccttt gccatagtag
     1381 ctgctacttt cgacaacacg aaagcccgca aacggtggga agtaatggcg cagcggatgt
     1441 ttttcgaagc tcgccagcag catctccccc agttcttcaa tgctcgcttc gttctcagga
     1501 ttgccaatgt tgataatttc gccatcgcag cgatttcccg cattttcgat aatgcggtat
     1561 aacgcctcga taccatcccg aatatcagta aagcagcgtt tttgttttcc gccatcaatc
     1621 agcttaatcg gtgaaccttc taccagattg agaatgagtt gcgtaatagc gcgggagctg
     1681 ccaattcgcg ctgcattaag gttatccagt cgtggcccca tccagttaaa cgggcggaag
     1741 agggtgaact gtaaaccttc tttttcgcca taggcccaga tcactcgatc aagtaattgt
     1801 ttagacaccg aataaatcca gcgtggttta ttcaccgggc cgacgattaa attagaatga
     1861 tcctcatcga agtatttatc gctacacatc ccataaactt ctgaagtcga cgggaagatg
     1921 attcgcttac ggtacttcac gcagtagcgg ataatgcgca gattctcttc gaaatcgagt
     1981 tcaaatacgc gcagcgggtt gcgggtatat tcaatcggcg tggctatcgc caccaaaggc
     2041 aagacgacat cacatttttt gacgtgatac tcaatccatt cggaatgaat actgatatcg
     2101 ccttcgacaa agtgaaaatg cggatgattc agaaaacggc ttatcgcatc gctgccaata
     2161 tccagaccgt aaacttcata atgatcttcg cgcagcaggc gttctgtcag atggttgcca
     2221 ataaagccat tcaccccgag gatgagtacc cgggtacggc gtcgggcggc acaggcaggc
     2281 tggctattca agcgtgaacc ttgcaccagg cccagcgtct gcgctaattg cgagccctgc
     2341 atagtaatgc cgtcgcccgt ctgtccagtg acgatttcca gcgcgccatc gccacaggca
     2401 atcagcagtg gtgcaacaga aatcacgctc cccggctgtg ctttgctggc atgaggatga
     2461 acacgcgacg accagacggt gaatttctga ttgccaacat agctgaaggc acccggccac
     2521 ggatcggcaa cggcacgcac catgttgtgc agtacggatg ccgacttatg ccattcgagg
     2581 aaactgtcat ccggcgttct gcgaccaaaa caggtggctt cgttttcgcg ctgggcgatt
     2641 tccagaatat taccgtgttt aatggcgggt aatgtctttt ccagttgccg gcgcgcggcc
     2701 tggcacaatt tatgatgcag cgtaatggcg atatcgtctg gcgcaatggc aatgcgcagt
     2761 tgcgccacaa tggccccgga atcggcacgt ttcaccattc ggtgcaatgt aacgccagtt
     2821 tccgtttcac cgttcaccag cacccagttc agcggcgcgc gaccacgata ttttggtaac
     2881 agcgaaccat gcagattaaa tgcacctctc ggagcgagct gcaaaatctc atcgcaaata
     2941 agatggcgat aataaaaaga gaaaatcacc tctggtgata gttgggcaat gcgttccacc
     3001 cacagcggat gattaacgtt atccggcgca taaactggaa tgcctctttc cgctgccagt
     3061 cgggcaaccg aaccataaaa ggctttttca ccggggttat cagtatgggt aaaaatggcg
     3121 ctaatttcgt aaccggcaga cagcagggct tcaataccga ggcatcccat atcgtggtag
     3181 gcaaaaacga cggttttcat tattcatttt ccttgctgga tggacggata acttgctgaa
     3241 caaaatagcg ggggcgggcg cggacatcgg tgtagatcct gccgatatat tcaccgagca
     3301 atcccatgcc gataaactga gcgccaataa aagtaaacag cacggcaaat agcataaaga
     3361 cgccttctgc cgcccattgt ggtccgaagg tcaggcgtaa aatcaccagc agcaccgcaa
     3421 tgctaaaacc gccaatcgca ataatgctgc cgagcaaact caacatacgt agcggcgttg
     3481 tggtaaggca ggtcaccagg tcgtacatca aattaatcag gcgcataaag ctgtatttgg
     3541 attcaccaaa ctcacgctcg gcatggtgca ctggaatttc aatggcacgg cgggcgaaga
     3601 tattcgccag aatcgggata aaggtgctgc gttcatggca gtgcaacatc gcatcgacaa
     3661 tatgacggcg ataggcgcgc agcatacaac catagtcacc catcgctttg ccagtggtgc
     3721 gctgaataag ccggttaatc atcttcgaag cggttttacg aaaccagctg tcctggcggt
     3781 tctggcgtac agtccctacc acgtcgtaac cttcatcggc ttttgccacc agacggggga
     3841 tttcctctgg cggattctgg agatcggcat cgagggtaat aattaagtcg ccggtgacgt
     3901 gactgaatcc cgccataatc gctgaatgtt gcccgtaatt gcggttaagc aagatagaca
     3961 caatatggct gccctccgct tgtgaggctt cgaccagcac atgcgcggaa ttatcgctac
     4021 tgccgtcatc aatcagcagg atttcatact ctttccccag tgattcacag gctgcggtgg
     4081 tgcgcctgat taattccggt aagctttcct gctcgttata aacgggaata accaccgaga
     4141 ctttcttaac agggtggatt tcaaacatgg cttattgtcc tgcgagttgc tgaagggctg
     4201 tgatgacgcg gtcggcatcg gcggtggtca tatccgggaa cagcggcaac gaacagatgc
     4261 gttcactatt ccattcggta ttcggtaacg acagcgtggg aaaacgcccg cgataatatt
     4321 tttgtgtgtg agcggcgcgg aaatgtaacc cggtaccaat gcctctttct tttaacgctt
     4381 ccatcaacgc atcgcgactg ataccacaac gttgttcatc gacacgaata ataaacagat
     4441 gccaggcgtg aacgtgcggc caggcgggaa ggcttaatgg ctgaaagggg agagctgcca
     4501 gtgcttgctg atattgctgg gcaatttcgc gccgccgggt gttgaggtgc tctaatttga
     4561 ctaactgtgt cagggcaatc gcggcgttaa tatcggtcag attgtactta tagcccggcg
     4621 ttaagacttc agcctgcggt gcacggcccc aggtatgtct gtcataggca tcgacaccca
     4681 gaccgtgaaa tttcagcatc cgcagctggc gggcaagatt ttcattatca gtaacaatca
     4741 ggccaccttc cgcacaggta atatttttga tggcgtgaaa cgaaaaaata gcggtacctt
     4801 ttgcgccaat atgtcgccct ttgtaatacg taccgacggc atgggcagca tcttcgataa
     4861 ctgcgatgcc gtaacgttcg ccaatggcgc gaatggcgtc aatatctgct ggcgcaccgg
     4921 cataatgcac cggaatgatg gctttagtgc gtggtgtaat ggctgcttcg atcgcttcag
     4981 gcgtgaccat cagcgtatcg cagtcgacat ccaccattac cggcgttgca cccagcaagg
     5041 aaatcatatt gagggttgaa acccaggtca gggaaggcgt aatcacttca tcgcccttgc
     5101 caatttccaa cgccattagc gtgatatgca ttccggcggt ggctgaactg accgcgatgg
     5161 catgctgatt tcccgtcaac tggcaaaaag cttgttcaag cgcctgattc ttcggaccgg
     5221 ttgtgatcca accggattcg agaacctctt taactgcagc gagttcctct acgcccattg
     5281 ctggtcgcga aaaaggcaaa aatcctgaca ttgtttttcc atccgtcatt gaatacctgt
     5341 ccacttatat ttgctataaa gagtgttgtg tatattttgc catttggagc aaaattttaa
     5401 ggatagaata ttaacttaac cttaagaaac taatattaga cgtaaatatt gaaattttta
     5461 tattttttct tat
//
LOCUS       DAWDRC010000233         5468 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6670_length_5468_cov_2.781637, whole genome shotgun sequence.
ACCESSION   DAWDRC010000233 DAWDRC010000000
VERSION     DAWDRC010000233.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6670_length_5468_cov_2.781637"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3392..3401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3896..3905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1327 a         1374 c         1457 g         1290 t
ORIGIN      
        1 tgctttttat taaaaatatt ttaataaacg gtaatgttga tcatattttc aaatgaaaat
       61 cctcagtaag ctgcccgccc ttttttacac tttcaggagt gtgttatggc agcttcaacc
      121 ttttttattc cttccgtgaa tgttattggt gctgattcat tgaatgatgc aatgaatatg
      181 atggcagatt atggatttac ccgtacctta attgtcaccg acaatatgtt aacgaaattg
      241 ggtatggcgg gtgatgtgca aaaagcactg gaagaacgca atatttttag cgttatttat
      301 gatggcaccc aacctaaccc caccacggaa aacgtcgccg caggtttgaa attacttaaa
      361 gaaaataatt gcgatagcgt gatttcctta ggcggtggtt ctccacacga ctgcgcaaaa
      421 ggtattgcgc tggttgcagc caacggcggt gacatccgcg attatgaagg cgttgaccgc
      481 tctgcaaaac cgcagctgcc gatgatcgcc atcaacacca cggcgggtac ggcctctgaa
      541 atgacccgtt tctgcatcat cactgacgaa gcgcgtcaca tcaaaatggc gattgtggat
      601 aaacatgtaa ctccgctgct ttctgtcaat gactcctctc tgatgatcgg tatgccgaag
      661 tcactgaccg ctgcaacggg tatggatgcc ttaacgcacg ctatcgaagc gtatgtttct
      721 attgccgcca cgccgatcac tgacgcttgt gcactgaaag ccgtgaccat gattgccgaa
      781 aacctgccgt tagccgttga agatggcagc aatgctaaag cgcgtgaagc aatggcttat
      841 gcccagttcc tcgccggtat ggcgttcaat aatgcttctc tgggttatgt tcatgcgatg
      901 gcgcaccagc tgggcggttt ctacaacctg ccacacggtg tatgtaacgc cgttttgctg
      961 ccgcatgttc aggtattcaa cagcaaagtc gccgctgcac gtctgcgtga ctgtgccgct
     1021 gcaatgggcg tgaacgtgac aggtaaaaac gacgcggaag gtgctgaagc ctgcattaac
     1081 gccatccgtg aactggcgaa gaaagtggat atcccggcag gcctacgcga cctgaacgtg
     1141 aaagaagaag atttcgcggt tctggcgact aatgccctga aagatgcctg cggctttact
     1201 aacccgatcc aggcaactca cgaagaaatt gtggcgattt atcgcgcagc gatgtaatca
     1261 tcatttccac aacggctggc aaattgtcag ccgtttttta tcaactatct caaaattttc
     1321 gatttttgag atagtttctc tagtctgcta tacactatct caatattgcg cttttttgag
     1381 ataagcaaat ggctatcaaa ttacctccca cgctgattcc tttatcacaa ttatcgggtg
     1441 aggaacttca ggctcatctg cgctttaacc gggtcaccga tgaaaaagga cgttttctgc
     1501 cttttgatga gttgcagtat cgcatcaaaa aaggagaaaa cgccgaagtg gcgtggacgc
     1561 ttacccggct ggcgcgcaac gcggcgatcc agcgaatcaa ttattgcaat gaagcaggtg
     1621 aacaggccgg attcaacatt acctcggtga ttgcagaagc gtgcgagctt gtcgacaaac
     1681 gtgctaccgc gctggcgctg aaagatcaga ctgaacggtt acgcggggcc ggagccgagt
     1741 taagccagtt acgcctggaa gagccgatca ccagctccca gctggaaggt gcgaatacta
     1801 caacgctggt ggcgagaaaa atgctggaaa cagggcggtc gccacgcact gaagacgagc
     1861 atatgattgc gggaaatgcc cggctgatgg cggaaattcc gcacttactc gcagaaccgc
     1921 tgacacctgc gcttattcgc cagttacatg ccattggtat gggtggaatc aatgacgcga
     1981 aatatcgtcc gggagaattt cgtgaaacag atgatgttgt gatcgccgac tatgatggca
     2041 atatagtgca tcaaccgccc gctgcggtat tgttaccgga acggctggaa aaagtttgcc
     2101 agtggttaaa cagtcatgaa ggatatattc acccgttaat ccgtgcctgc attctgcatt
     2161 ttatgctggc ccatgaacat ccgtttcgcg atggcaacgg gcgcaccagt cgggcgctgt
     2221 tttactggta tatgctgaaa tcgggctacg atgtttttaa atatatttcc atcagccgtt
     2281 tgcttcatgc agcgccagta aaatatgccg ccagctacca gtacactgaa tcagatggta
     2341 tggatctgac ttatttcctt gaatatcagg cgggtgtcat taaacgtgct ctgcaaaact
     2401 ggcagcagca tatcgatgaa attacacaac gaagcgcaaa actcgatagt gtgctgttta
     2461 gctctggtgt gcttaaacgc ctgaatccgc gccaggtaac gctcctgaat gtgatgctcg
     2521 ccaatccggg gaaagagtac acagttgctg aaattagcgc gagcttgggc gtctctgata
     2581 atacggctcg agctgattta cggacaattg ttaaagaagg attcgcgcaa gagaaaagaa
     2641 ttaataacca gcaagcagtg tacgtcgcac actacccgct gtagcttttg cccgtaaatt
     2701 cgtgatagcc gtcgtaaagc tgttaccgac tggcgaagat ttcgccagcc acgtctaccc
     2761 ttgttatacc tcacaccgca aggagacgat catgaccaat aatccccctt cagcacagat
     2821 taagcccggc gagtatggtt tccccctcaa attaaaagcc cgctacgaca actttatcgg
     2881 tggcgaatgg gtagcgcctg ccgatggcga gtattaccag aacctgacgc cggtgaccgg
     2941 gcagctactg tgcgaagtgg cgtcttcggg caaacgagac atcgatctgg cgctggatgc
     3001 tgcgcataaa gtgaaagata aatgggcgca cacctcggtg caggatcgcg cggcgattct
     3061 gtttaagatt gccgatcgaa tggaacaaaa ccttgaactt ttagcgacgg cagaaacctg
     3121 ggataacggc aaacccattc gcgaaaccag tgctgccgac gtgccgctgg cgattgatca
     3181 ttttcgctat ttcgcctcgt gtattcgggc gcaggaaggt gggatcagtg aagtggatag
     3241 cgaaaccgtg gcctatcatt tccatgaacc gttaggcgtg gtggggcaga ttatcccgtg
     3301 gaacttcccg ctgctgatgg cgagctggaa aatggctccc gcgctggcgg ctggcaactg
     3361 tgtggtgctg aaacccgcac gtcttactcc gnnnnnnnnn ntgctgctaa tggaaatcgt
     3421 cggtgatgta ctgccgccag gggtggtgaa cgtggtcaac ggcgcaggtg gggaaattgg
     3481 cgaatatctg gcgacctcga aacgcatcgc caaagtggcg tttaccggct caacggaagt
     3541 gggccaacaa attatgcaat acgccacgca aaacattatt ccggtgacgc tggagctggg
     3601 cggcaaatcg ccaaatatct tctttgctga tgtgatggat gaagaagatg cctttttcga
     3661 taaagcgctg gaaggctttg cactgtttgc ctttaaccag ggcgaagttt gcacctgtcc
     3721 aagccgtgct ttagtgcagg agtctatcta cgaacgcttt atggaacgcg ccatccgccg
     3781 tgtcgaaagc attcgcagcg gtaacccgct cgacagcgtg acgcaaatgg gcgcgcaggt
     3841 ttctcacggg caactggaaa ccatcctcaa ctacattgat atcggtaaaa aagagnnnnn
     3901 nnnnnagggc gctgatgtgc tcaccggcgg gcggcgcaag ctgctggaag gcgaactgaa
     3961 agacggctac tacctcgaac cgacgatttt gtttggtcag aacaatatgc gcgtgttcca
     4021 ggaggagatt tttggcccgg tgctggcggt aaccaccttc aaaacgatag aagaagcgct
     4081 ggagctggcg aacgatacgc aatatggcct gggcgcgggc gtctggagcc gcaacggtaa
     4141 tctggcctat aagatggggc gcggcattca ggctgggcgc gtgtggacca actgttatca
     4201 cgcttacccg gcacatgcgg cgtttggcgg ctacaaacaa tcaggcattg gccgcgaaac
     4261 ccacaaaatg atgcttgagc attaccagca aaccaagtgc ctgctggtga gctactcgga
     4321 taaaccgttg gggctgttct gatgtgaaag gctgcccggc gtgtattgcc gggcactatc
     4381 aggtatggct actgcccata ataggcattg gccccatgct tacgcaggta gtgcttatcc
     4441 agcagttcgt tttgcatcgc agggagctgt ggcgcaagct ggcgcgagaa cagacccata
     4501 taggcgcact cttccagtac cacggcgtta tgcaccgcat cggcggcatt ttttccccag
     4561 gcgaacgggc cgtgagaatg caccagcacc gcagggattt gtgccgggtt caacccacgc
     4621 tcttcgaagg ttttaatgat cacttcgccg gtctggtatt catactcgcc gttgatctct
     4681 tctgcggtca tcaggcgcgt gcaggggatg gctccgtaaa aataatcggc gtgagtagtg
     4741 ccccaggcgg ggagatcaag tccggcctgc gaccagatgg tggcgtggcg cgagtgggta
     4801 tgcactatgc cgccaatctc cgcaaagcga cggtaaagcg ccagatgcgt tggcgtgtcg
     4861 gaagagggct ttttgctgcc ttccaccacc ttgccgctgg ctatctcaac caccaccata
     4921 tcgtcggcgg tcattacctc atactcaacg ccggaaggct taatcaccat ccattgccgc
     4981 gtttcgtcta tcgcgctgac attgccccag gtgaacgtca ccagatgatg aacgggaagc
     5041 gccaggtttg ccgccagcac gtcagctttc agttgctcta acatataaat ccggcctcct
     5101 gcatacgcgc ttcaatccag cgccgcgcct gaataatctc cagcaccggc tctttggctt
     5161 tttcggtcca catttcaatc agaaatgatc cgcgatagtt cagctcatgc agcgttttga
     5221 agatgccaac aaaatcgaca cagccttcgc caaacggcac atcgcggaac tgtccggggc
     5281 tatgttcggt aacgggttga gtgtctttca ggtggatggc ggcgatacgg tcgataccca
     5341 gtttcagttc ggcgggaaca tcattgcccc aggcgctgag gttgcctacg tctgggtaaa
     5401 cggtgaacca cggtgacgca agcatttcgt cccacttttt ccatttgctg atagagttca
     5461 taaacgcg
//
LOCUS       DAWDRC010000234         5467 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6673_length_5467_cov_2.784368, whole genome shotgun sequence.
ACCESSION   DAWDRC010000234 DAWDRC010000000
VERSION     DAWDRC010000234.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6673_length_5467_cov_2.784368"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    720..729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1392 a         1350 c         1496 g         1219 t
ORIGIN      
        1 gaccagatcg cccatcagcg tggtcatcac gtcgttgagg gtgatgatcc ccactaccag
       61 tgcgtactca ttcatgatca ccgcgaagtc ttcacctgcg gttttaaaac tttccaacgc
      121 ctctgaaagg gttaacgtat ccggcacaat cagcgtattg cgaatttgta cgccgctgtt
      181 cagtgccagg ctttggttag ccagcacgcg gttcagcagg tctttagaat cgacatagcc
      241 gatgatgtgg tcaatatctt cattacagac gagaaactta gagtgcggat gttccgccac
      301 cttattcttc agactttgct catcttcgtg gagatcaaac caaatcacgt tttcacgcgg
      361 tgtcattgaa gacggaacgg tacgggattc cagctcaaag acgttttcaa tcagctcgtg
      421 ttcctgttta cgtaacacgc ccgccagcgc accggcttcc actaccgcgt agatgtcatc
      481 agaagtgatg tcatctttac gtaccattgg cagtttgaaa atacggaaga ttatgttcgc
      541 cagtccgttg aagaaccaca ccagcggggt gcaaacgtac aggcagaagc gcatcgggtt
      601 gatgatacgc aaagccacag cttctggcgc aatcataccg atgcgtttcg gggttaaatc
      661 cgcaaacagg ataaacatgc cagtcactaa cgagaaagag agaataaagc ccaatttccn
      721 nnnnnnnnnc atatagcggg agaacaggct gtgaaaagct ggagaaaatg ccgcatcacc
      781 gacgataccg ccgagaatcg ccactgcgtt cagaccgatc tggaccacgg taaagaacat
      841 gccgggattt tcctgcatat tcagaacgcg ttgggcattt atattgcctt catcagccag
      901 cagtttaagt ttgattttgc gtgaggcggc aagcgagatc tcggacatcg agaagaacgc
      961 acttacagcg atcaaacaga gtatgactaa aatactgttt aacatatctt atccgacctt
     1021 tcaggccaga tcctcggaag gggaagttga ttaatttgtg tgaaatacac attgaaagcc
     1081 gattcgaaga aagtgaaccg gcattttcag tggctagtat agcgtaaggt actgtaaagc
     1141 cgccagaggg ttaaaattca gacagctgaa aaatgaaaaa ctgcctgatg cgctatgccg
     1201 aacaggccta cgcagtcccc gcaatatatt gaatttggac gattttgtag gccgggcaag
     1261 gcgtttacgc cgtatccggc ataaacaaaa tgcactttat caacaatcta catttgtacc
     1321 cgtaacgcta tgcttccggc ggcagacaga cgccaattcc gccaattcca cagtaaccat
     1381 acgggttttt atgcagatat tgctggtggt catcttcggc ataataaaac ggtgtggcgt
     1441 tagcgatttc cgtggtgatg cgacgatcgt catcggcggc aagcatcgcc gcctgaaaac
     1501 gttccagact ggcgcgagcg gcggcatcct gttctggggt cagcggataa atcgctgaac
     1561 gatactgcgt gccgtggtca ttgccctgac gcattccctg ggcgggatcg tgattctccc
     1621 aaaatacttg tagcaactgc tcatagctga tgacggatgg atcgtaaaca atgcgcaccg
     1681 cttcggcatg gccagtatcg ccggagcaca cttcccgata agtcggattt ggcgtatagc
     1741 cgccggtata gcctgcggcg gtgctgtaaa cgccgggtaa ctgccagaac agacgctcca
     1801 caccccaaaa acaacccatc gcaaaaatgg caatctccat tccgtcaggt acattggtca
     1861 ttgagtgacc gttgaccgca tgcagtgtgg ctacgggcat cggggtgtta cgtccaggca
     1921 gggcatcggc gggggaaacc agatgctttt tatcaaataa actcatggtg tcgctctccc
     1981 gaaaatcggt cattggggtt aaggttgtaa caagaggcgt atttgcacac aataaccacc
     2041 gtgaataggt ctaaagtaaa acataagaaa tattttgggt ttagtctgct ttttaatcca
     2101 tattactgga tttttgttaa gccgtttaac ggcgttccag gggcaggaaa aaaggatatt
     2161 caggagaaaa tgtgcgctat atccgacagt tatgctgtgt aagcttactc tgcttaagcg
     2221 gatctgccgt cgccgcgaac gtccgtctac aggtcgaggg gttatcggga gagctggaaa
     2281 agaacgttcg cgcgcagctt tctacgattg aaagtgatga agtgacgcca gaccgtcgct
     2341 ttcgcgcacg cgtcgatgat gccatccgcg aaggtctgaa agcgctgggc tattaccagc
     2401 cgaccattga atttgatctc cgtccaccgc caaagaaagg gcggcaggta ttgatcgcca
     2461 aagtcacgcc aggcgtgccg gtgttaattg gcggcaccga tgtggtattg cgcggcggcg
     2521 cgcggaccga taaagactat ttgaaattgc tcgatactcg cccggctatt ggtacggtac
     2581 tgaaccaggg cgattatgaa aatttcaaaa agtccttaac cagcattgcg ttgcgtaaag
     2641 gttatttcga tagcgaattt accaaagcgc agctgggcat tgcgctcggc ctgcataaag
     2701 ccttctggga tattgattat aacagtggcg aacgttaccg ctttgggcat gtgacctttg
     2761 aaggatcaca aatccgcgat gaatacctgc aaaatctggt gccgtttaaa gagggcgatg
     2821 agtacgaatc gaaagatctg gcagaactga accgtcgact ttctgctacc ggctggttta
     2881 actcggtggt ggtggctcca caatttgata aagcgcgcga aacgaaagta ttaccattga
     2941 cgggcgtggt ttcgccgcga acagaaaaca ccatcgaaac cggcgtcggt tactctacgg
     3001 acgtgggacc gcgcgtgaaa gcgacgtgga aaaagccgtg gatgaactct tacggtcaca
     3061 gtttgaccac cagtaccagt atttccgcgc cggaacagat cctcgacttc agctataaaa
     3121 tgccgctgct gaagaatccg ctggaacaat attatttggt gcagggcggt tttaagcgca
     3181 ctgacctgaa cgatactgag tctgactcca ctacgctggt ggcttctcgc tactgggatc
     3241 tctccagcgg ctggcagcgt gccattaacc tgcgctggag tttcgaccac tttacccagg
     3301 gtgaaattac caacaccacg atgctgtttt atcctggggt gatgattagc cgcacgcgtt
     3361 ctcgtggtgg cctgatgcca acctggggcg actcgcaacg ctactctatc gactactcca
     3421 acacggcctg gggctcggat gttgatttct ccgttttcca ggcgcagaac gtctggatcc
     3481 gcacactgta cgatcgccat cgttttgtga cacgcggcac gctgggctgg attgaaacgg
     3541 gtgatttcga caaagtaccg ccggatctgc gtttcttcgc cgggggcgat cgcagtattc
     3601 gcggctacaa atacaaatct atcgccccga aatacgccaa cggtgacctg aaaggggcct
     3661 cgaagctgat aaccggatcg ctggaatacc agtacaacgt gaccggaaaa tggtggggcg
     3721 cggtgtttgt cgatagcggc gaagcggtaa gcgatattcg ccgcagcgac tttaaaaccg
     3781 gtaccggtgt cggcgtgcgc tgggaatcgc cggtcgggcc aatcaaactc gattttgccg
     3841 tgccggtcgc ggataaagac gaacacgggt tacagtttta catcggtctg gggccagaat
     3901 tatgagttta tggaaaaaaa tcagcctcgg cgtggttatc gttatcttac tgttgctggg
     3961 atcggtggcg tttctggtgg gcaccaccag cggcctgcat ctggtattta aagcggcgga
     4021 tcgctgggtg ccaggactgg atattggcaa ggtcaccggc ggctggcgtg atctcacctt
     4081 gtctgacgtt cgttatgagc agccaggcgt ggcggtaaaa gcgggcaatc tgcatctggc
     4141 ggtcgggctt gagtgcctgt ggaacagtag cgtttgtatt aatgatctgg cgctgaaaga
     4201 cattcaggtc aacatcgaca gtaaaaaaat gcctccttct gaacaggttg aagaagagga
     4261 agatagcggt ccgctggatc tctccacgcc gtatcccatt accctgacac gggtggcgct
     4321 ggacaacgtc aacatcaaga ttgatgacac cacggtatcg gtgatggact tcacctccgg
     4381 cctgaactgg caggagaaaa ccctgaccct gaaaccgaca tcgctgaaag gcctgttgat
     4441 tgctttgccg aaagtggcgg aagcggcaca ggaagaagtg gtcgaaccga agattgaaaa
     4501 tccgcagccg gaggaaaagc cgctcggcga aacgctgaaa gatctctttt ctcgcccggt
     4561 attgccggaa atgaccgatg tgcatttgcc gcttaacctg aacattgaag agtttaaggg
     4621 cgagcagctg cgcgtgacgg gcgacacgga catcaccgtg agcaccatgc tgctgaaagt
     4681 gagcagcatt gacggcaata ctaaactgga cgccctggat atcgactcca gtcaagggat
     4741 cgtcaacgcc agcggcacgg cgcagctgtc agacaactgg ccggtggata tcaccctcaa
     4801 cagcacactg aacgtggacc cgttgaaagg tgaaaaggtg aagctgaaag tgggcggcgc
     4861 gctgcgcgaa cagctggaga taggcgttaa cctttccggt ccggtggata tggatttacg
     4921 cgcccagacg cgactggcgg aagccggatt gccgctcaac gtggaagtga acagcaaaca
     4981 gctttactgg ccgttcactg gtgagaagca gtatcaggcg gatgatctga aactgaaact
     5041 taccggcaaa atgaccgatt acgcgatctc tatgcgcacg gctgttaagg gccaggagat
     5101 cccgccagcc accattaccc ttgatgccaa aggtaatgaa cagcaggtca atctcgacaa
     5161 acttaccgtc gcggcgctgg aagggaaaac ggaacttaag gcgttgctcg actggcagca
     5221 ggccattagt tggcgcggtg agctaacgct taacggcatt aacaccgcca aagagatccc
     5281 ggagtggccg tcgaaactca atggcttgat taaaacccgc ggtagcctgt acggcggcac
     5341 ctggcagatg gaggtgccag aactgaagct gaccggtaac gtcaaacaga acaaagtgaa
     5401 cgttgacggc acgctgaaag gcaacagtta tatgcagtgg atgatcccag ggctgcatct
     5461 ggaactc
//
LOCUS       DAWDRC010000235         5467 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6674_length_5467_cov_2.002217, whole genome shotgun sequence.
ACCESSION   DAWDRC010000235 DAWDRC010000000
VERSION     DAWDRC010000235.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5467)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6674_length_5467_cov_2.002217"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    519..528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1303 a         1397 c         1556 g         1201 t
ORIGIN      
        1 tcctagcgcc ggacataacg cgcacctgga aaatcccgtt ggcgtaatcg caagtctggc
       61 gcagatcttg cgtttctgac taaaggacac aatatgattt atcctgatga agcaatgctt
      121 tacgcaccgg ttgaatggca cgactgctcc gaaggtttcg aggacattcg ttatgaaaaa
      181 tccaccgacg gtatcgcaaa aatcaccatt aatcgtccgc aggtgcgcaa tgccttccgt
      241 cctctgacgg taaaagagat gatccaggcg ctggcggatg cgcgttatga cgacaacatc
      301 ggcgtgatca ttctgactgg cgcaggcgat aaagcgttct gctccggtgg tgaccagaaa
      361 gtgcgtggcg attacggcgg ctataaagat gattccggcg tacatcacct gaacgtgctg
      421 gacttccagc gtcagatccg tacctgtcca aaaccggttg tcgcgatggt ggctggctac
      481 tccatcggtg gcggtcacgt cctgcacatg atgtgcgann nnnnnnnntc ttcggtcaga
      541 ctggcccgaa agtcggttcc ttcgacggcg gctggggcgc ttcctacatg gcgcgcattg
      601 ttgggcagaa aaaagcgcgt gaaatctggt tcctgtgccg tcagtacgac gcgaaacagg
      661 cgctggatat gggccttgta aacaccgtgg tgccgctggc ggatctggaa aaagaaaccg
      721 tccgttggtg ccgtgaaatg ctgcaaaaca gcccgatggc gctgcgctgc ctgaaagctg
      781 cactgaacgc cgactgtgac gggcaggcag gtctgcagga gctggcgggc aacgctacca
      841 tgctgttcta tatgacggaa gaaggtcagg aaggtcgcaa cgctttcaac cagaaacgtc
      901 agcctgattt cagcaaattt aaacggaatc cgtaatgcgt agcgcgcagg tataccgctg
      961 gcagatcccc atggacgcgg gggtggttct gcgcgacagg cggttaaaaa cccgcgacgg
     1021 gctgtatgtt tgcctgcgtg agggcgagcg cgaagggtgg ggggagatct ccccactgcc
     1081 gggcttcagt caggaaacct gggaagatgc gcaaagtgtg ctgcttgcct gggtaaataa
     1141 ctggctggca ggcgattgcg aattaccgca gatgccttcc gttgcctttg gcgtaagttg
     1201 tgcattggcg gagctggcag agacgctccc gcaggcggcc aattaccgtg cggcaccgct
     1261 gtgtaatggc gatcctgacg atctgatcct caaacttgcc gatatgcctg gcgagaaagt
     1321 ggcgaaggtc aaagtgggat tgtacgaagc cgtgcgcgac ggcatggtgg tgaatctgtt
     1381 gctggaggca attcctgatc tgcatttgcg tcttgacgcg aaccgcgcct ggacaccgct
     1441 gaaaggccag cagtttgcca aatacgttaa cccggattat cgccaccgca tcgcgtttct
     1501 cgaagagccg tgcaaaaccc gcgatgattc gcgtgcgttt gcccgtgaaa ccggcattgc
     1561 cattgcctgg gatgaaagct tgcgcgagcc ggattttgcc tttgtagctg aagagggcgt
     1621 gcgcgcggta gttatcaagc ccacgctcac gggcagcctg gataaagtac gcgagcaggt
     1681 gaaggcggcg catgcgctgg ggctgacggc ggtgatcagc tcttccattg agtcgagctt
     1741 aggtctaacg caactggcgc ggattgctgc ctggttaacg ccagacacca ttccgggact
     1801 ggacacgctg gatctgatgc aggcgcagca ggttcgtcgc tggccgggga gcccactgcc
     1861 gttagtggac gttgatgcac tggagcggct gttatgatct tctctgactg gccgtggcgt
     1921 cactggcggc aagtgcgggg agaagccatc gccttacgtc tcaatgacga gcaactcaac
     1981 tggcgcgaac tttgtgctcg cgtcgatgaa ttagcctcca gctttgcggc gcagggtgtg
     2041 gttgagggca gcggcgtgat gttgcgggcg tggaatacgc cgcaaacgct gctggcctgg
     2101 ctggcgttac tgcaatgcgg ggcgcgggtg ttgcccgtga accctcagct gccgcaaccg
     2161 ctgcttgagg aattgctgcc caatcttacg ctgcaatttg cgctggtgcc ggaaggggga
     2221 aacacgtttc cgtcgttagc gttgctgcgc attcagctgg ttgaaggcgc acatgccgcg
     2281 gcgtggcagc cgacgcgtct gtgctcaatg acgttaacct ctggttccac tggcttgccg
     2341 aaagccgctg ttcatactta tcaggctcat cttgccagcg ccgaaggggt gttatcgctg
     2401 attccgtttg gcgatcacga tgactggttg ctctctttac cgctgtttca cgtctctggt
     2461 cagggaatta tgtggcgctg gttatacgct ggtgcgcgga tgacggtgcg tgataaacag
     2521 ccattggagc aaatgctggc aggttgtact cacgcttcac tggtgccaac acaactctgg
     2581 cgtttgctgg ttaaccgtag ttccgtttcc ctgaaagcgg ttttacttgg tggcgcggct
     2641 atcccggtcg agttgacgga acaggcgcgc gagcagggga ttcggtgctt ttgcggctat
     2701 ggtctgaccg agtttgcctc cacggtgtgt gcgaaagaag ccgacggtct ggcagacgtc
     2761 ggttcgccgc tgccgggtcg ggaagtgaaa atcgtaaaag acgaagtgtg gctgcgggct
     2821 gccagcatgg cagaaggtta ctggcgtaac gggcaacggg ttccactggt taatgacgaa
     2881 ggctggtacg ccacgcgtga tcgcggtgag atgcataatg gcaagctgac cattgtcgga
     2941 cgtttagaca atctattctt cagtggcggt gagggtattc agccggaaga agtcgagcgc
     3001 gttattgctg cacatcctgc ggttttgcag gtgtttatcg ttcccgttgc cgacaaggag
     3061 tttggtcatc ggccggtagc ggtggtggag tatgaccagc aaagcgttga tcttgacgaa
     3121 tgggtgaaag ataagctggc tcgttttcaa caaccgatgc gctggctgac tttaccgccg
     3181 gagctgaaaa acggcggtat taaaatttca cgtcaggcgc taaaagagtg ggtgaaacgc
     3241 cagcaataat taaccctccg tttcacttct catttctgcc attccattgc cgttgataaa
     3301 gagtttatct gttaatctgt aaattgatgt gaaaactctt agcaaacagg ataatgcaat
     3361 ggaatggctg gtcaaaaaat cgtgctgcaa taaacaagat aacagacatg taattatgct
     3421 ctgcgatgct ggcggcgcga taaaaatgat cgccgaagtg aagagtgact tcgccgtgaa
     3481 agtgggggat ttactctcgc ctttgcagaa tgcgctttac tgtattaatc gtgaaaagct
     3541 gcacacggtg aaagtccttt ctgcaagctg ttatagcccc gatgagtggg aacggcagtg
     3601 taaagcggca gggaaaactc agtaacgttg ttttgttgtg ggcaggaaaa tcagcatcaa
     3661 cccgctcata atacaggcta ctcccagtag tgctttcagc gaaaaagtcc cttcccagcc
     3721 gggaagtacc attgaagcaa tccataccag cacataactc atactgagca aggcataggc
     3781 tttgcttagc gcaagtttat gtagtgtttt ataccaacaa aaaacggaaa gcagataacc
     3841 cagcagcccg agtaacagta tccgggcatc caggccaaaa gctaacagcg ccgcgataaa
     3901 atcccataag tgcgtcattg gcggcagatg actcgccgca aaacccaggc ttaattgcgc
     3961 agcggaggcg ataatgacgc tgaataatcc ccacatcagg cccattacac cgtactcccg
     4021 aggatcacaa tgccgccaat aatgaacgcc accccacacc agtgacgcag cgataccggc
     4081 tcgtgccaca gttttactgc agccagcgtt acccagacaa aattcagact taacatcggg
     4141 taggcaatgc ctaccggtac gttctgcaag accagcagcc agagcaccat ggcaaggcca
     4201 agacaagcca gcgccagtcc cagccacagc acgatatgtt tgcgccgttt gtttaccgcc
     4261 gcaaagcagg ttgcctgttt ctgacacaac tgcccggcaa cgctaagcaa gctggcaaag
     4321 actaatgtta accagatcat ttgggccgat actgaattag caccagacgc tcctgacgat
     4381 cgatgacatc tgcgggcgga atggcgagac tgttgatatc ttcatcgcgg tcaaccgaaa
     4441 gcacgagtgt aataatcccc tcctgacgat gttgattaag ccagtttgcg aactcatcac
     4501 cgctgacaaa tctccctttt gcatccggat aattaaggcc atatttcaac tcacctgtct
     4561 ggcgatacat gatgatgtca tcgcgttgca ggctccatgc cagacctgcg gcaacaccga
     4621 cgctatcagt aagaatatag cggcttggct gaagtgattc ttgtgtcatc tcgacaaaaa
     4681 attgcggatg ttttccttcc atgactctgt caggaattga aaatcctacc agcaacgcca
     4741 gccccagcgg gcaaagcgcg gcaaaaggcc aggtcttttc gacgtttgtt aaggtgtacc
     4801 agccgaaaaa agcccatagc gaaaaaatag accaggcaca aaagacttta tagctctcga
     4861 aggtttgcca caccggtgtg ttcattggtc cccaggggga gaccacaaat gtggcaataa
     4921 tgccagtgac gccaaaagcg atgttaatcc agccattaat ccgcaacgcc agaggattat
     4981 tttttgctgc cagcaacgcg taatgcgcca tcagcattgc cagaggtgca aagcaggaaa
     5041 gaatataggt gggcaatttg cctttagcga cggagaaaaa cagcagcggc attatcgtcc
     5101 agctcaacaa atagacggtt gcggaatgct tgcggttttt ccagccagcg tacagtgcac
     5161 cggggagtaa tcccagccac ggcaggctac cggcaatgat gactggcaag tagtaccaga
     5221 acggcgctct atgttgggca tcatccagtg caaagcgttg aatatgctca acccagaaaa
     5281 aatagtgcca gaagtcaggc tcccgctgcg ctatcgccag tccccagggg agaaccgtca
     5341 gtacgcaact gataaccgcc agccagccgt aaataaagag atctttccag cgtttttgcg
     5401 ttgctaccca tggcagcacg cttaataccg gcacggcaag ggcgagaaaa cctttggtca
     5461 tcacccc
//
LOCUS       DAWDRC010000236         5450 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6695_length_5450_cov_2.468211, whole genome shotgun sequence.
ACCESSION   DAWDRC010000236 DAWDRC010000000
VERSION     DAWDRC010000236.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6695_length_5450_cov_2.468211"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1253 a         1536 c         1360 g         1301 t
ORIGIN      
        1 tgataccgca accgcgtccg gcagttttgc cagccagcgg cggcccactt catcgtcggc
       61 gttaataatc gcctgaccgc aatgatgtgc agaataaagc agccatttcg cggcttcgta
      121 gtgttccata tcaccatgat aatcaaggtg atcgcggctt aagttggtaa agaccgacgc
      181 cgcaaatttc aatgccgcca cacggtgttg taccagcccg tgggatgaaa cttccatttc
      241 gcaaaaagtc gcgccctgat ccaccagccc cgccagctca tgctgaacat cgactgccga
      301 accggttgta ttttctgtcg ggatcacttt ccccagcagg ccgttaccaa cggtgcccat
      361 taccgcgctg gtttcgccaa gcagttggct ccactgcgcc aacagctggg tcgtcgtggt
      421 tttgccgttg gtgcccgtta cgcccacgag acgtaaattg tcagagggtt catggtaaaa
      481 gcggcccgcc agtgcagata aacgctcgtt gagctggctg agatagatga ccggtacgcc
      541 gtgcatttca cggatttcac cgtcggtcgc ctcatctttc gcctctgcaa taatggcagc
      601 cacaccttgc gctatcgcct gcgggatata tcgacgcccg tccgcctgat gacctactac
      661 agctacaaag agatcgcccg ccgcagccac acggctgtcg agtgtcatct ctcgcagtgc
      721 tcgcgaaggt gcgtctggca cccacggagc aagaaggtcg cgcaaattac gatctgccac
      781 ctgtaccctc gccttgatta atcacaaatt catttttatc gcccgttgtc agcgcatccg
      841 gctcgatgtt catggtacgc aatacgccgc ccatgatggc accaaagacc ggcgcggaaa
      901 cggcgccgcc gtagtattta cccgcctgcg gatcgttgat aacaacaacc agcgcgaagc
      961 gcggctgact cgcaggcgca acgcctgcgg tataagcaat atatttattg atgtagcgac
     1021 cgtccggccc gacctttttc gcggtaccgg ttttaatggc gatacgatag cctttaatcg
     1081 ccgccttcac gccgccgccg cccggtagcg ccacgctttc catcatatgc accacggtgc
     1141 gaacaatgga ttccgggaag acacgttcac cgggaaccgg ggggtcaact ttggtaatcg
     1201 acagtgggcg ataaatgccg tagctgccga tagttgcgta gactcgcgct aactgtaacg
     1261 gtgttaccat tagcccgtag ccgaaagaga aggtggccct ctctatgtca gaccaccgtt
     1321 gtttttgagg atataagcca ctgcgttctc cgaccaaccc caaattggtc gcttttccca
     1381 gtccaaaacg tgagtaagta tctactaacg ctgaggacgg catcgctaac gccagtttgg
     1441 aaacaccgac gttactcgac ttctgtaata ccccggtcag ggttaattcg ctgtagcgcg
     1501 ccacgtcttt gatttcgtga ccgttaattc gataaggaac ggtattgagt accgagtttt
     1561 cccgcaccac gccacgttgc aacgcggtca tcaccaccat cggtttaacc gttgagcccg
     1621 gttcaaacac gtcggtgatg gtacggttac gcatcgcttc tttcggcgtg ccgctcagat
     1681 tatttgggtt gtatgacggg ctgttagcca tcgccagcac ttcaccggtg ttgacatcca
     1741 ccagcacggc gctaccagat tcagccttgt taaaggccac tgcgttgttc agttcgcgat
     1801 aaaccagcgc ctgcaggcgt tcatcaatac tcagcgtcag gttgtgcgct gcctggctgt
     1861 cagtagaaga aatatcttca attacgcgac catagcggtc tttacgcaca atgcgctcac
     1921 ccggctgccc ggtaagccat ttatcgaaac tcttctcgac gccctcaatc ccttggctat
     1981 cgacgttagt aaagccgatg aggtgagcag tcacttcgcc ggatggataa taacggcgag
     2041 attcttcacg cagatgaatc cccggcagtt tcagtttttt gatgtagtcc gccatgtcag
     2101 ggtttacctg acgcgccaga taaataaagc gccctttcgg gttggcgtta atgcgggctg
     2161 agagctgatc cagcggaata ttgagcgcgt tagccagcgc cttccagcgg tcaccgacgc
     2221 tgataccgcc agcgtcatgc acttcttttg ggtcagccca aatcgctttt actggcacac
     2281 tcaccgctaa cgggcgacca gaacggtcag taatcatgcc gcgggaggtg gaaacttgct
     2341 gaacgcgaag agaacgcatg tcgccctctt tcaccagcat atccggggag ataacttgta
     2401 accacgctac gcgtccgagc agaaaagcca gcgccaggag aatacagccg cataacaacg
     2461 caaaacgcca actgataaag ttggcatgtt cttcctgacg ttttggtttc tgcgttttcg
     2521 ccgctgcttt catgcgtcgc gtttatcctt atttttgcac tacgatattt tcttgtgacg
     2581 gatcaacatg ctgcatttgc agcttttccg tggctatcct ttccacccgg ctatggtcgc
     2641 cgagcgcatt ctcttcaagg atcaggttgc gccattcaat gtctaaagca tctcgctcca
     2701 gcaccagttg ttcgcgctga gcggtcagta aacgggtatg gtgcgccgtg gttaccacgg
     2761 tcaccgccgt caaaataatg caaatgaaca ggcagagtgg cagcttccca aatcgcaaaa
     2821 gatcgtcacc gataacgcca ggcaaggcat ggcgctcgtg gcttcccatc gatcctttaa
     2881 ctttgcttag agcttctgtc actctgctga tcatgcgttc gtcctctctg caatacgcag
     2941 aactgaacta cgggcacgag ggttctcagc cacctcttct tcgcccggca tcaacttacc
     3001 tagtgctcgc aactgacggc cccccagttt tttgagctgc tcttctgtca tcggtaaccc
     3061 tgccggaact tgcggaccgc ggctgttttc acgcataaaa cgtttcacaa tgcggtcttc
     3121 cagcgagtgg aagctgatga tcgaaagccg cccacccggg gccagcacat tgagcgagct
     3181 ttttagcgcc tgctctatct cctccagttc actgtttacc caaatgcgca ccgcctggaa
     3241 ggtacgggtc gcgggatgtt taaacttatc tttcaccggc gttgcggcag ccacgacttc
     3301 cgccagttct ttggtgcggg tcatcggctg ttcgcggtta cgctcgacaa tggcgcgggc
     3361 aatgcgtttg gcaaaacgct cttcaccata ggttttcaat acccaggcga tatcggcttc
     3421 ttctgcggtt tgtagccatt cagcggctga ctgcccacgg gttgggtcca tacgcatgtc
     3481 cagcggacca tcgcgcataa aggaaaagcc gcgctcagca tcatcaagtt gcggtgaaga
     3541 gacgccaaga tcgaggagaa tgccgtcgat cttgccgata agatcgcgct cggcaacgta
     3601 ttcgcccagc gcggagaaag gtccgtggat gatggagaag cgcggatcat caatagtctt
     3661 cgcaacggcg atagcctgcg ggtcgcgatc gatcgcaaac gcccctcttc gccaagctgc
     3721 gagaggatca gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca
     3781 ggacggatat tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt
     3841 tccatcattt tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg
     3901 ctctgcgtcg atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa
     3961 cttgttgaac tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac
     4021 tggcgcgatt aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag
     4081 taggcgctgc acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc
     4141 gataatttcc cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt
     4201 gcaaaccatt tgaccggcag cgttctcaag cagctgttcc cgataacggg taggcactga
     4261 taagcgccct ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc
     4321 ccttattccc actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa
     4381 gcttgtcaag ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat
     4441 cagcgttgtt tattttacgt ccaacgtgcg aagcaaatta acgttatgaa tagctgtaag
     4501 aaaaaatccc tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat
     4561 aaagtgtcag tttgcgacgc gagcggcatt ttaggacata tcttctctgg ttaacagtcc
     4621 ctgttgcgtc tgtgtggcgc ttgatgccag caggtttgcg caaaaacaaa gaaaagtgtt
     4681 tgttaaatcg gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcaata
     4741 tcatttaatt attgtgaatt tcagcaggtg agttaattaa cgggcgggtt taaaagcatt
     4801 tatcaataat agccaggaat agtcttattt actttaagga tattgatgtc cattcccgta
     4861 ctcaacgcgc cagagagaaa ttcacctggc gcgtactttt gttcgcggct tagctacggc
     4921 tgagcacgcc gcggcgatag agattacgtt taatgcgcgt taaaccaggt tttggcttac
     4981 gcggttcgtc caggctcgcc aggacaatct ccagcacacg ttctgcgaca tcgcggtgac
     5041 gttgagccac tgccagcacc gggcactgta agaagtcgag cagttcgtta tcgccaaagg
     5101 tggcaattgc caggtcagaa ggcagtttgc cgtcgcgacg tagcgtgaca tccatcaccc
     5161 cttgcaacaa cgcaaacgac gttgtgaaca gcgcctgcgg catcggatgc gtttccagcc
     5221 atttttcgaa taactgggca gctgcctccc gctcatagct gttggcataa aggaaatgca
     5281 cttcgcgcgg atcatctttc caggcagtac ggaagccttg ttcacgcagg aagctgacag
     5341 aaagctccgg cagcgcgcca agataaagca ccgtctcggc gggaaactta cgtaacacct
     5401 ccgccagcat ttcggcatca tcctgatcgg caccaaccac gctggtgaag
//
LOCUS       DAWDRC010000237         5430 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6725_length_5430_cov_2.686512, whole genome shotgun sequence.
ACCESSION   DAWDRC010000237 DAWDRC010000000
VERSION     DAWDRC010000237.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6725_length_5430_cov_2.686512"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1611 a         1177 c         1115 g         1527 t
ORIGIN      
        1 cattattagt aacaatgata atctggctgt atcgaaaata attctaaact ttcaggagat
       61 ttattatgaa taaacgtatc ctcacgattg cgccattctt cttaccgata ttctgacaca
      121 catcattaac tttctatttt tactcttttc tccaccataa aatcaataaa agcacgaact
      181 ttagagggca ttctcacccg tgatgaaaca tatatataaa atccaggaaa tgaaacggcc
      241 caatcctcaa gaatcggctg taacttgccc gcattgatat gttcagcgat tgcaaaatcc
      301 agatgttgaa tgatacccgt tccattcagc gccattcgta tcatgctttc gtcatcatta
      361 gaaatatact tacctgacac ctctatttcg aactcttcat taccccccac aacattcgaa
      421 aactcccatg ctgagagtgc accgctactt ttatgccgca accgaagaca attatggtta
      481 ctcaattcct gaggagtttc aggaataccg tgttctttta aataatctgg cgatgccaca
      541 gtaaccagtt taataagcgg agaaacagga acggcagtca tatgctcatc cagtccttgc
      601 tcaagacgta tgcccacatc acagccgtcc gcaataatgt tagataaacc gtcatccata
      661 atgagttcta tctttgtttt ggggtaacga gtaagaaact cgccaatatg aggctcaata
      721 agcatccttg ctgcaacacg ggaggaattt attctcaaca gtcctgtagg ttcaagttgg
      781 gtatcaccga catctttcaa cgcatcatca atttgcccca aagccgacac cagcacttca
      841 tacagatgct gtccttcctc agtaagcgac atattgcggg tcgtacggta tatcagcttg
      901 gtgttaagac gttcctcaag agattttaaa ttctgggaca atgcggcccg cgtaataccc
      961 atttcagagg ctgctctgga aaaactgccg gcacgaacaa tcagcgcaaa ccaggcaaga
     1021 gaaggcaaaa ttgaaggctc tattttcatc attgtaaacc ttaacttaac actgtgatag
     1081 atattagacc attgatgata accaatacaa gtcatactat tacgccgttc gcgatatgaa
     1141 cacgaatgcc agttgcggat aatagaaatg atatttaaat ctgtttaggc aaatccctgg
     1201 agcttgaaat gaaaactgtt tcgataaaac acacttactg ggatatagct gctgatatct
     1261 atttccctga cgattttaat agtgaaaaaa agtatcctgc cattatcagt gcccatccaa
     1321 ttggaagctg caaagaacag acatcgggta gtgtgtatgg tgccgccctg gcaaaagctg
     1381 gctttatcgt cattgctttt gatgccagtt ttcagggaag cagcggtgga gaaccgcgtt
     1441 atctggaaga tccaacaatg cgagtaaaag acttcagcat tgtcgttgat taccttacca
     1501 ccttacctta cgttgatgcc gggcgtattg gtgtactggg tatttgtggt ggaggtggat
     1561 atgccattaa tgccgcaatg acagaacgtc ggattaaagc cattggtact gtcacaggtg
     1621 caaactacgg gcgtcttatg cgtgagggat ttacagcgtt taatcctatc ggagcactcg
     1681 aagcaatggc gcaacaaaga acagacgaag ctaacggcgc aaaacttcgc gtagatgatt
     1741 tgctcccttc ttctccggaa gcagctcttg aagcgggatt aacagaaata gacctttttg
     1801 aagcaacaga atattatcgt tcgccacgag gctgtgcgcc taatggggta aatcgttcgc
     1861 tattttcaca tcagactgtt gccgtaggct gggatgcttt tcatcttgca gaagtcctgt
     1921 taactcaacc actaatggtt gtagtgggcg atagagtggg cgcttttggt gcctatcgtg
     1981 acggctgcga aattattggt cgtgcagcct caaaacataa agagctggtc gtggttgaag
     2041 gttattcaca ttacgacctg tatgataagc cagaacctgt gaaacaggcg ctcgataaat
     2101 taattccttt ttacaaaaca catttgtaat aaccttgcag ggagattcat tatctccctg
     2161 acatttatcc tttattgact acacgacaat aataaaatta ctcaaaaaga attccattaa
     2221 aggaaaaaag catcaaaaag tagcaactca acttattaag attatacgca ttaaaatcag
     2281 ataaacattt gaggtattgt tatgacaaat aaacacccat cattgttttc tccttttatg
     2341 ctgacagaaa aaataaagct acgtaatcgt atcgttatgg ctcctatgac aacctggtca
     2401 gctaatcctg atggaaccat ctctgagcaa gaactcgaat tttataaacg tcgctcacaa
     2461 aatgttggtt tagtaataac aggatgtacc tatgtcacgc caagcggcat tgggtttact
     2521 catgaatttg ccgcgtatga cgaccgtttc attaacagcc ttgaaaagtt agcagctgca
     2581 gccaaaagtg gcggtgctcc ggctattttg cagattttcc acgccggtaa taaagccatc
     2641 ccggagcttg tgccaaataa cgatgttatt agtgccagcg catcaagcgt aaaatctggt
     2701 gactttatga agagagtggt acaaagcagg gaaatgactg aaaacgaaat tcaggaaacc
     2761 attagagcat tcggagatgt cacaaaaagg gcgattaaag caggttttga tggcgttgaa
     2821 cttcatggtg cgcacggatt ccttctgcag aatttcttct cccctctatt taaccagcgt
     2881 aatgaccgat ggggaggaga tcttgaagga cgtatgcgct tcccgctggc cgtattacag
     2941 gaagtaaaaa atgtagtcta tgaatatgcg acaaagccct ttgctatcgg ttctgtaaca
     3001 ggggggctgc ggatagagga tacttataaa ctccttgatc ggctcatttc atccggtatt
     3061 tcgtatatac atacctcgct ggtcagtatt aacgatagct accctgttga gtcgccaaat
     3121 ggcccccgaa ctattgaatt gattttaaat catattgcag gcagagtgcc ggtaatcgcc
     3181 gcaggtaaaa tacgcacacc gtctcaggct caagaagcaa tttccgctgg cctgccactt
     3241 gttgcgatcg gtaaagggct ggtgataaat ccagaatggg tgactttagc tgaatcaggt
     3301 agaagtcatg aaattcaaac agcccttaat ccacaacgcg ttcctgaatt aaccattccg
     3361 gataaactat gggatcaaat tcaggcatcg aaaggaactg gctggtttcc gttaatggat
     3421 taaatatcga ttactctccc gagagtaaaa ctatcaaatc aatgagtaac cgatacagcc
     3481 tcgcaaattc tttcagataa ttaacccgtc actgcggcac gttctctcgt aaggcgtgcc
     3541 tgcagtgtaa tccagtatgt atccggcata gttgacggtt taatagactc aattcctctc
     3601 cctggttacc tacaacgata tcttgtataa caccccagga gatacgacgt tacgatgaga
     3661 tgcgatatgc tcctcagcgc caatcaacgc taggactggg ccagtcttga gatcagactc
     3721 attcccgtaa ctgatttcag gcgacatata tcaatgacat tggggcaata aaagatagcc
     3781 gatgtaatac atctattcaa atttgtgata catgtcaaaa aattggttga ccaaactcgt
     3841 tatttatata agggcactta cgaagtgcac tcttttttaa agcgaggaag taccaatgaa
     3901 agagaataaa gtacagcaaa tcagtcataa actgattaat atcgttgttt ttgtcgcaat
     3961 tgtagaatac gcctatttat ttctccattt ctattaataa cggcaataaa ctgttcactt
     4021 cagtgatatt taaaatatgc atcctctccc ttttttgtaa gtaattatta tatccgtggg
     4081 agaggaatac acattgtcag gtaatcaatc atgctgcaat aaatcatcgg ccagtaaagt
     4141 ggagatagcc tccattctcg aaaaatccat actctcagcg aaaccatcat caatcactca
     4201 tccaggcgtt tatgggagcg tcgccaatgg ctgctaacaa tgccagactt ccccgttgcg
     4261 gaaattccac atcccacaaa tagtcacagt gattgggtgt tgaaatgatc cggatgagca
     4321 tgtatcttta cggttatgtt ataacataac aggtaaaaat gatgaaaccc aatatccatc
     4381 ctgagtatcg tactgtggtg ttccacgaca ccagcgttga tgagtacttt aaaatcggct
     4441 cgactatcaa aacagaccgt gagattgagc tggatggcgt aacgtatccg tacgtaacaa
     4501 ttgatgtctc ttcgaaatcg cacccgttct atacagggaa gctgagaaca gtggcatcag
     4561 aaggaaatgt tgcacggttc acccaacgtt ttggtcgttt tgttagcacg aaaaaggggg
     4621 catgatgaaa gtccttaact ctctgcgtac cgcaaaagaa cgccatccag actgtcagat
     4681 tgtgaagcga aaaggacggc tgtatgtgat ttgtaaatct aatccacgtt ttaaggccgt
     4741 tcagggtcgt aagaaaaaac gttgatttaa aattcgacga attaacgata tttgtctgat
     4801 taataatcag accggattaa tgttggtgta tttattacac cagcattaat tttcctggag
     4861 atgtatcttc ctgttcattt aaggtcaact gcctgacgtt tctctccgaa tattccatta
     4921 tattaatgtt gacttgttga ccagctgcgc cctgtatgct ggcatcaact ctattttaga
     4981 ctgaacacgc cactcagttt cctccctttg cgacgcagcc tgcatttaca ctcaaactgt
     5041 taagatgata aatgtggtaa atctgttggt actgacataa aaacgtttac gccacaggaa
     5101 cggccagatc catcggtaac cccatcgccg acgttcgagt gccagttaga gtaacgcgta
     5161 cagatgactg aatgcagtgc cctggcaaag aggccatcgt tcctgtgaca actggcagtc
     5221 ttcgtttaac ttcacttaat ttggctcttg gggggcttac cggacagatg acgtacttac
     5281 gcctgtttaa tttttcatca cttattggga tgaacaccca taaccatttt gtgcggcatg
     5341 ttaatccatt aaaacacctt actgattggc aaatcatctt taattatgac ttatgatagt
     5401 tttatattct atttcttatc atttaaactt
//
LOCUS       DAWDRC010000238         5402 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6766_length_5402_cov_2.767533, whole genome shotgun sequence.
ACCESSION   DAWDRC010000238 DAWDRC010000000
VERSION     DAWDRC010000238.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6766_length_5402_cov_2.767533"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    255..264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    986..995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1748..1757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1209 a         1366 c         1515 g         1282 t
ORIGIN      
        1 acggcagtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc ggtctgtttg
       61 tcgctcagac cacgcagtcg tgcccaggtg acgtgacgct gaccggcgac gtccagcata
      121 ctggcgcgca gcaaacgcgc gttaatcgcc agcgacataa aggcgatgga taccgcaggc
      181 aaaatgatgt gctgccagcc gccgtaaccc attgcgggta gccattgcag atacaccgaa
      241 aacgccatca ccagnnnnnn nnnngagcag aattaatacc agcgccgcac ctgcaagttc
      301 cagcgtggcg ggcaggaaat tcagcatatc gtccagcacc gggcgttggc tggcgaatga
      361 gataccaaag tcaagatgca gcgccttcca caaccaggtg ccatactgga cgtacagcgg
      421 ctgatccagc cccagcatag ttcgagtaga ggccagcatc tccggcgtcg gtggcaggtt
      481 agacagacgg agataatcga gcgccgggtc accggtgccg aggcgcagca tcaaaaaaat
      541 gatcaccgag gcggcgagca ccatcgggat cagcagcaga aagcggcgta atacataacg
      601 caacattaag gtttcaccgg tttaatctgt tcgaacggga tttcggtggc gatcggcgcg
      661 taggggatgt tacccagttc cggttttgaa accaccatca ttgagatgta actgataggc
      721 agataaaccg cctcgtcatg cagacgggtc agaatgtcgc gatacagcgc ctgacgttgc
      781 gtttcgtcat gggtcgccag cacttcgccg atctctttat caatcagcgg cttgtcggct
      841 aaaccttgct gtgcctggaa gtcagcgtgc gacggtacgc gcattgaact caggaaggcg
      901 tgtggatcgt atggcgcgcc ccaggtgcgg tggaaaatca tgccaaaacg accgtcgcgc
      961 tgacgagcat agatactgct ctcttnnnnn nnnnngctct cttcttcgcc aatcagcgag
     1021 acatctgcgc caatctggcg catatcagcc tgaatgattt ccgccatcga tttgcttaac
     1081 gcatcggtgc cgatgaacga cagttcaatg cgcagcggct gaccattttt ctcgcggatg
     1141 tctttgccag caggcagcgt ccaaccggct ttttccagca acgctttcgc tttctgcgga
     1201 tcgtactggc gcggtttcag gccgaggttg gcgtagggca cagaaggggc aaacagggtg
     1261 tcggcgacct gctgggtacc atacaacgcg ttatcaatca gcgatttttt gttcaccgcg
     1321 taattaagag cttcacgtac tgccagctcg ttggtggggg ctttggcggt attgagcgcc
     1381 agcatcacgg tttcgatcgg ctgtgacagt tgagtgtggt aagccgggtt ctggctaaag
     1441 cgggcgaagg tatcgagcgg taataaccct tcgtttccgt acagcaggtc gatatcgcca
     1501 gtttcaaacg ccaccgcgcg ggtagtcggg tccgggatca cgttaaaggt gatcttttta
     1561 atcgctggct tttcgcccca gtagttttcg ttacggacga agacatcgta ctgattcagt
     1621 ttcgattcct gtaaaaccca cggcccggtg ccaatcggcg ctttaattcc gttcatggtt
     1681 tcgtggtttt taaactgcga gggtgcgata aaacggaaag gacggggcag ggccagttct
     1741 tgcaagannn nnnnnnnttg caagaaagga tagtaggcgc ttttcagggt gatttgcagc
     1801 tcgtttttac tgagtgcttt gacatcaaca atctggtttg ccagctccag ccaggcgtga
     1861 cgttgacggt tatcgagcac tgcacggaag ttttctgccg ccgcctctgc atcaaatggt
     1921 tcgccgttgg agaacttcac gtcatcacgc agggtgaagg tccaggtttt accatcttct
     1981 gaatgagtcc agctttttgc cagccacggg atcaccgaac cgtctgcctg atatttcacc
     2041 agcggttcat aaaccatgct ctgggcgaac atttggttag gcgtgtaaag gtgcgggttt
     2101 agtggcccga cattcaccgg ccaggcggtg gtgatttcat ctggtgcagc ggcatggacg
     2161 ataaaagacg cacaagccag cagcgcaaat agagtgcggc ggagtgtaga gaacatggta
     2221 accccaatgg attaaaatag atggcggaaa taagtatgac gattttaagt attcgtcata
     2281 ctgattacct gttctggatc aatagatggg cactcgggtg ggcggatcag ttaactgaat
     2341 cgatccattg cacactgtcg gcggtgagcg taaagggggt aggggtgcag atcgccaggc
     2401 ttaaattttc tagctgacaa tggctgactg acagcgagga gttataggtg ctgtctacgc
     2461 tgacgatttg ccaggcgctg ccgccacgct gtttaacgat ggcttctttg cgcgtccaga
     2521 tgcgccagaa catttccagt tgctgatcag gatgcacggc gtccatctcg gcatgttccc
     2581 cgaggctgaa tacggcgttc gccagccagc gccagttggc gcgcgggcga atcacttcga
     2641 tatcgcagcc gacttcgcct tcatcgctca acagcagggc gatatcgtca ccactatggc
     2701 ttaagttgaa ccatagcggc gtttccggcg cgaatgcagg tttgccttgt tcgccataga
     2761 tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgc acgccccgcc agccagcgtt
     2821 cacgtcgtgg accttgcggt gcttgctcgc gtaaacccgg tggcagagga gctgcgctta
     2881 aggtcgaaac tttccccaga actatccgat acatatcagg gccaacgttt aatggaaaat
     2941 gaaagtgcgt atcgtaacac ttgtcgcctc atcccggtaa ccgacttttc ggtctgcctg
     3001 gccccagtaa aatcgccagt ttgctgccgc ctttggtggt ttccatccag attttacaca
     3061 cgctggttaa tggcaccgaa agtagcatcc ctaccgggcc gagcagccat ccccaaatta
     3121 acaatgaaag aaataccacc atggtggaca tccccaggcg atggcccatc atccgtggtt
     3181 ctaaaatatt gccgatgacc atatggacca ctaaaaacaa tgcgccgacc agaatacatt
     3241 cgtaaatacc attaaacagc agcacctgaa tcattggcgg tacggcggaa attaccgcac
     3301 cgatattggg cacgtagttg agcaaaaacg ccagtactgc ccacatcagc gcaaactgca
     3361 cccccatcag ctccagcccc agccagacga ttatgcctgt ccataaactg agtagcgtct
     3421 ttaacgccag ataatgcgaa acgcctttaa gtgcgcggtg tagtccggcg atgtgaatct
     3481 gtggattatt caacgcgaaa cgcattttgt aagggacgtg gcgcacttca aacagcataa
     3541 aaactacggt catcaccagc aaaagcacgc tcgccattgc cccggaaagc ccggtcatta
     3601 gcgccgtggt gaaggtaacc actttttccg agtccatccg ctgcagtatt cgctccggcg
     3661 acatatgcaa attaagaaaa ggcaacatct cctgcaattt aaaaagtttg cgcgtcagct
     3721 ccttattaaa cctcggcagc atagagataa attcgttaaa cgatgccgcc agtacgccga
     3781 ccagcgcggt gagtgcgatc agcatcacca caaccacaat cgtaatggca acggggcgtt
     3841 gtaccccccg acgaataaac caggtgacga gcgggttgag aacgatggca aaaaacagcg
     3901 ccagtaatag ctgcacaatg atatctgccg ctgcgtgaat gcccgcgagg atcactacca
     3961 gcgaggccaa cttgagcaga atgtgcatgc ccgttttatc gggttgaggg gtttccattg
     4021 gggcttcctt gtgacttttt gtattaagtg tagcgggagt cactccagca atattctgat
     4081 tcttactgct gattttcacg tcagcacatg gtaaaaatga aacactgttg taaaaatgtg
     4141 gtgatcctca tgcccgaacc cgtagccgaa cccgcgctaa acggattgcg cctgaatttg
     4201 cgcattgtct ccattgtcat gtttaacttc gccagctacc ttaccatcgg gttgccgctc
     4261 gctgtattac cgggctatgt ccatgatgta atgggattta gtgcattctg ggcagggttg
     4321 gttatcagcc tgcaatattt tgccaccttg ctgagccgtc ctcatgccgg acgttacgcc
     4381 gatttactgg gacccaaaaa gattgtcgtc ttcggtttat gcggctgctt tatgagcggc
     4441 ctgggatatc tgacggcagg attaaccgcc agtctgcccg tcatcagcct gttattactg
     4501 tgcctggggc gcgtaatcct tgggattgga caaagttttg ccggaacggg atcgaccctg
     4561 tggggtgttg gcgtggttgg ttcgctacat atcgggcggg tgatttcgtg gaacggtatt
     4621 gtcacttacg gggcgatggc gatgggtgcg ccgttaggtg tcgtgtttta tcactggggc
     4681 ggattgcagg cgttagcgtt aatcattatg ggcgtggcgc tggtggccat tttgttggcg
     4741 atcccgcgtc cgacggtaaa agccagtaaa ggcaaaccgc tgccgtttcg tgcggtgctt
     4801 gggcgcgtct ggctgtacgg tatggcgctg gcactggctt ccgccggatt tggcgttatc
     4861 gccaccttta tcacgctgtt ttatgacgct aaaggttggg acggtgcggc tttcgcgctg
     4921 acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat tccctaacgg cattaaccgt
     4981 atcggcggct taaacgtggc gatgatttgc tttagcgttg agataatcgg cctgctactg
     5041 gttggcgtgg cgactatgcc gtggatggcg aaaatcggcg tcttactggc gggggcgggg
     5101 ttttcgctgg tgttccctgc attgggcgta gtggcggtaa aagcggttcc gcagcaaaat
     5161 cagggggcgg cgctggcaac ttacaccgta tttatggatt tatcgcttgg cgtgaccgga
     5221 ccactggctg ggctggtgat gagttgggcg ggcgttccgg taatttatct ggcggcggcg
     5281 ggactggttg caatcgcgtt attactgacg tggcgattaa aaaaacggcc tccggtggaa
     5341 atacctgagg ccgcctcatc atcttaaaac ttactgaata accagcgtat taatgatgtt
     5401 tt
//
LOCUS       DAWDRC010000239         5399 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6770_length_5399_cov_2.434132, whole genome shotgun sequence.
ACCESSION   DAWDRC010000239 DAWDRC010000000
VERSION     DAWDRC010000239.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6770_length_5399_cov_2.434132"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1415..1424
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1853..1862
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4286..4295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1261 a         1365 c         1373 g         1370 t
ORIGIN      
        1 caggcttgat gcttgggtat atttgctgat ttagcaggga tttttgtgga tcggacttca
       61 gattgataaa ggggacgccg cgcagctccg acgacagcat aaaaatcttg ccgccaattt
      121 taaaaacatc gaactccggg ccaaaaggcc agcaaagctc gacaaagggt aactcaagag
      181 ccaggcgttt cgccgtttcg tgcagtgatt gcttatccat aaatgttcct ttaggcgaag
      241 gagaataagc aaagtatgcc gcgaagttcg gcgataatcg acgtttaatc cgccagcgag
      301 aaccagcgtc gccagataaa gcgtagaaca aaatactcaa tagcgcccag cactaaaaac
      361 cacagacaaa acaataaagt gtaaagctgg ctaagatcca tcagatggaa cgtggtcacc
      421 agtttttgtg ccagcgttag ccccagtgcg ggggcgggca gcagcaggca agagaggaaa
      481 gccatcagca aaatgcctgc tgcggtggtc aaggtttcta aagggtgttt cataacattg
      541 ttaaatgtaa gttaaaacac cattgtcagg gatattcttc tgtaaggcaa ttcccgactt
      601 attctccggc ccagatctca caatgctttt tcaccagccc aattagcgag ccaccatcgg
      661 cgacgaaatc acgcattagc tgcgcttcat tcagaccact gacgacctga cggtgcagtg
      721 cttcaattgc gctcgatgcg ccaattttat gtgcagaagg ggtgattttt tccagtaaac
      781 gcaaggtggc ttccgttagt gaccgacgat cgccagtatg cggatcggta atgacgcctt
      841 ccagcccata gcgacaggcc tggaaacggt tgaatttata cagcaggtaa tctttctcct
      901 gatgtttaaa cgggcgttcc gtcagtaacc agtgggcggt ggcctgaatt aatcccgcca
      961 tatttaccgc gtggctaagg gttaacgggg tatccatcac ccgaacttcc accgtaccaa
     1021 aatgggggct ggggcgaata tcccagtgca gatctttaat gctgtcgatc atcgtggtgt
     1081 aactcaggca gcgaaacagg gcttcaaatt gttgccagtt actgacccac ggcatcgggc
     1141 cattatcagg aaaggcggaa aaaatattcg gtcgtgagga ggcaaaacgc gtatccgttc
     1201 cttgcatata tggcgacgcg gcggaaaggg agataaagtg cggcacaaac cgtgacaagc
     1261 cgtgcagcaa ataaatggcg tcatcgccac tggcgcagcc aacatggaca tgctgaccaa
     1321 aaacggtcgc ctgctggatg agatagccaa agttttgcag cgttcgttga tagcgttcgt
     1381 tatcgcacac ctcctgacgc tgccatttct gaaannnnnn nnnnctttcg gttatatcgt
     1441 gctttacctc tccggccgtg atctgatttt taaccgtgtc aatcagcatt gatgagtcct
     1501 ggcttaagtc atagcccggc ggattaacca cctgcatttc cagttcaata ccgagggtaa
     1561 aaggttcaga aacatgaaaa tcgggtaatg gcataggttt ctcttatgtt ggcgttttct
     1621 attcagtata gaagtgggag cggctgggtg agatgcggaa gttctggaat gtttcttttt
     1681 ttggtgatgg agaactgatt gtgactgaag caatttggct acttttgcaa tgtgacaagt
     1741 tatggcagag ctggctggcg gcgaagaatt ttgacgattg aggcatgcag aaaaaaacgg
     1801 gttcagcttc agttgattaa atgaggagtg agaagtccga aacaggattt acnnnnnnnn
     1861 nngaggtctg gaaagatgaa gccccaggag atatttctat caaccctggg gctgccactc
     1921 caaacccgaa caatttggat ggtagtccct tcttcgcatg gaggcaatat aaacatgctg
     1981 acgaaatatg cccttgtggc agtcatagtg ctgtgtttaa cggtgctggg atttacgctt
     2041 ctggtcggag actcgctgtg tgagtttacg gtgaaggaac gtaatattga gtttaaggct
     2101 gttctcgctt acgaaccgaa gaagtagccg ttgtgcgggg agtaatcccc gcatatccgg
     2161 ttgtcaggtc aggatggtaa ggcacctgct ttacactttc gcccgcagtc agagatggct
     2221 gcgggcgaat cgtaccagat gttgtcaatt aatcgtgttg gcacagcgtt atgactattt
     2281 tttcttttat ctgccagtgc acagcaaaca tctcattctc acgatgaatt atgacctgtt
     2341 gtttattccg gctaattatc tgatagtcca gaaaatctgc atcagtttgg atttcacttg
     2401 ccttaaatgc gctctctttg gcggaaaatg ccagtgtcag cgccagagga aaggctaaac
     2461 cgcagtctgc aagtcgcttg tgttccgctg gtgtaataat gttgtctgtc aattctcttg
     2521 cggtttgtgc agagaaaatc tcttcaatat caatgccaat cggttgacgg gaaaccacgg
     2581 ctaatgccgt agtcccacag tgactaatac tgccgtatac ctccgcaggc cacacaggtt
     2641 ggcgtagctc gccgattgcg ggcacacatt tatagccata ttcccgcagc gcataaactg
     2701 cagcgatccg cccggctaaa tgctctgttt tacgtttacg tccagcgtgt tgcagttgcg
     2761 cgtagtgcgg cagccagagt aaatcctgct cgcgaaaact cgccggaaca aactcaacaa
     2821 aatgcagcgt atgtccggca aaggggaggg cggtatgcgt agttttcatg tcgaccatat
     2881 tagagactga tgacaaacgc aaaactgcct gatgcgctac gcttatcagg cctacgtggt
     2941 cctgcaatat attgaattgg caagattttt gtaggccgga taaggcgttc acgccgcatc
     3001 cggcatgaac tacgcgcact ttgtcaacaa tctgacgtta gcatcagaag tgagtattaa
     3061 tgctcatata ccacgtacgt cccggctcgt tataggtata cgccccggca ccggcgatat
     3121 agttggctcc tgccagatcg cccgtggtct gggcattacc cgcacgccac aaacgtttgt
     3181 cgaacaggtt gtccacgccg ccggtcagac tgacattctt ggtcacatcc caggtcgcgc
     3241 tcaggccaac aatgctgtac ggactgattt ctttggtttc cggtccaacc gctggctgac
     3301 ctttatagtt gtacttcttc ggctgctgtt tgccgtacca ggtgaaggtc gtttgcatcg
     3361 acaaatcttc ccgtgcctgc cagctcagcg ttgagttcaa cgtatactcc gggatgatcg
     3421 acaaacggtc gcccgtggtt ttgttttcac tcttcagcat ataagtgatg ttattggtcc
     3481 acatcaccgt ttcgctaacc ggtacgttta acgatccttc cagaccttca accaccgctt
     3541 tcggtacgtt atcccactga tagagatcgg tgccgactgc gttttgccct acagccacat
     3601 agcccgcttc aatcttattg cgataatcgt tacggaacca ggtcacgccc gccagccagc
     3661 cgtcgcgttt gaactccaga ccaatctcct tgttgatgct ggtttctgct ttcaggtcat
     3721 cgttaccttg cagatagcag ccgcccgcgc tggcatagca gccctgacct ttactgtaga
     3781 gaatgtagtt tgggttagtc tggtacaggc tcggcgcttt ataggcgcgg gcgatgccca
     3841 ttttgagcgt gaagtcatcg cctaaacctt gcgatatgtt cagcgccggg ctccagttat
     3901 tgccgacaat actgtgatga tcgaaacgca gccccggcgt tacgatggtg ctgtcagtca
     3961 gctccatatt gttttcggca aacagcgaga aaatttctgc ttttgaatat gggctacggt
     4021 cggtggcact cacgccatca atagcaccgc cggtattcgt tccggtcagt gcctgggtgt
     4081 tggaactcaa gtccttcatc cgttgctgat tccactctgt acccagcgtc agcgtttggt
     4141 taacgaggaa atcaatcggc aggttaactt cgctgtgcag catcacgtca tcaagatcga
     4201 tatctacaaa atcctgtgcc gctttttcgt taaatttccc ttcggtaccg cccgccagac
     4261 cttccggaat acgcgagtta cgggtnnnnn nnnnngtgtt cgtactgcac ccagttgctg
     4321 gtggtcacgc cgttatccca gccaccgttc caggtcagcg agtagttctg gcgatacagg
     4381 cggttggttt catcgccata tttcgagcga gtgtaagcgt cagagttggt gttctgcgta
     4441 tcacccgcat acaggttacc ctggcggctg tagcccgctt ccagttcgag tgactgcaga
     4501 ggcgcgaagt cccagcgcac cacgccatta atatctttgt tgatcacccc ttcgcgcccg
     4561 gctggtaacg tcgtggcata cgttccggca cgcgcggact gatggccctg gttgatatcc
     4621 cacgcgtcag cctgggtttt gtcgaggttg ccatacaagc ggaagctgaa ttcgtcgccc
     4681 agcggaccgg tcaggctgaa gttggtgcgt ttggtggcac cttcctcttt atgttctggc
     4741 gcattgaaat aagcatccca ggagccgtgc cactcaccac tgcctttttt ggtaatgatg
     4801 ttaaccacgc cgcccgccgc gccgttgcca taacgcgcag ctgccggacc acgtagaact
     4861 tcaatacgtt caatcatttc aggcggcacc caggaggtat caccacgggt atcgcgctcg
     4921 ccacgccagc cctgacgcac agagttacgg ctgcttaccg gcttgccgtc aatcaaaatc
     4981 agcgtgtttt ccggacccat gccgcgaata tcaatctggc ggttattacc acgctgacca
     5041 ctggtggagt taccggtcag gttaacgcca ggcatggtac gaatgatctc cgatacatcg
     5101 cgggcaaccg ggtttttgcg gatttcatct gcggtaatgg tcgaaacgcc aggtgcctgc
     5161 aagttctgct cggcggcggt aacaacaata gtatcgtcat gtgaaacagg agtatcggtc
     5221 ggctcttgtg cctgtgctac cccataaatc cccagattga ccaacaaggc cagggaatga
     5281 atcttcttgt tcattgtttt attcctgcat ttttgccacg aattgcaact gtcgggcatg
     5341 gtcgtcatca acacgacgca tcccgctacc gcgaaaacct ttgatcctga aagacacgc
//
LOCUS       DAWDRC010000240         5394 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6781_length_5394_cov_2.014797, whole genome shotgun sequence.
ACCESSION   DAWDRC010000240 DAWDRC010000000
VERSION     DAWDRC010000240.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6781_length_5394_cov_2.014797"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1801..1810
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3400..3409
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1249 a         1439 c         1411 g         1275 t
ORIGIN      
        1 agtagcaaca ggtgcattcc agaaatttgg atttgcagtt cctgtaacag caaatgctgg
       61 agcgggttcg caggcaacat taccccgact tcgttaaggt caggggcgat atcatcacaa
      121 agctcaggta catatttttt atccaccagc acaatcggcg cggcgggcgt ggtaagcaac
      181 tggcgcgcag cgtctggtaa accgtcagcc actggcaaca tgaccgccag cggtttcgcc
      241 gggcgatgtt tgcgcgcccg aagtgtcgcc accgcgttac tgttacgtgc atcgcaggca
      301 agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa ctgtgcgata
      361 gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca ttcaagatgg
      421 gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag cgggtcacag
      481 tactctttgt cacaagccgg acacagcgga aacgccgcca tcacggtaaa cgggcggtcg
      541 taaggcatgg cgcgaataat ggtgaaacgc ggaccacagt gagtacagtt gataaacgga
      601 taacgccagc gtcgttcgcc tggggtattc atttcggcaa ggcaagcagg gcaagtggcg
      661 gcatcgggaa caatttgcgt attcatgacg ccacccgcgc tctggcggat agtgaactcg
      721 gtgggcagtt gtgaccagat aaacgactca cgctcgacgc tatcaatacg cgccagcggc
      781 ggacaatgct gatgcaattg aacaagaaac gtttccggat cttccagcag ccggacttct
      841 acgccatcgc cgtcattaca gacatcgccg tgaagattta attgctgtgc cagttgccag
      901 acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg gacaccgcaa
      961 gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc cgccctgccg
     1021 gacacgacag cgtcgcatcc ggcagtcaca ggtcggcgat actgcccgct tcgtattcta
     1081 cgaatatttc cgggaattcc tttgatacca gaacagttct gtaagatttt tagaacatca
     1141 gcgccgtgcg gcggcgtttt tctgcgctca gttgttcaag tttattacga tcgacacaaa
     1201 tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga tggttgcaca
     1261 ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagccctgcg ccgctgttgc
     1321 gaatcaccgg acgcaccacc acttccatcg caccatacgg gcaagccaca acgcaggttt
     1381 tgcaaccaat acaacgttcc tgcatgacat gaacaaaccc tttatcacgg ctgatagcac
     1441 cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact gtcgccgtgg
     1501 aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg gtcagcgatg
     1561 cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta cggcaaccaa
     1621 tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc aataacagtt
     1681 aatgcgccga tacattcaca aatcatgcca gtttttaatt tactgttatt taaggaaatt
     1741 aaattctgta ctacaggaaa aacgatgtca tcgacactag tgtcgatgac atgtgatgac
     1801 nnnnnnnnnn cctgaatcat ctcagtcacc ggaattttta cgctggagag cgccggaacg
     1861 atgtaggggg caatagcgat atcatcgaat ccgataactg acacctgctc tggcaccgct
     1921 acgccgcgct cgtgtaacgc tttgatcgca cctatcgcca tatcgtcgtt actggcaact
     1981 aacgcgctaa atttagcccc acgttcgagc aacgtttcta ccccttcggc accgctggcg
     2041 ggcgtccatt taccgttagc gataagtttt tcattgaacg caataccatg ctgcgccagc
     2101 gcgtctttat atccggcaag acgttcaatg ctggtggggg aatccatcga gccggtaagg
     2161 aaagcaatct cctgatgtcc ggcggttatc aactctgcca cggcgttaaa gctggtctgt
     2221 ttatgatcgc accagacgct atggctgctg tttttgcgca ggcggcgatt aagcaccatt
     2281 atcggctgac tgtgtgcgtc aatgatgtca tcaatctcat ccacgcttaa aaagcgcggg
     2341 taaatcatga tggcgtcgca gcgcaaatcc agcagatact gaatcgcctg gcgctcttct
     2401 tcggcgctat gtttaccatc tgccaatagc aactgtcgcc ctttctcttc cgccattcgc
     2461 gcggcatgaa acagtaattc actaaaataa atgccatggt aaagcgtgtt ggtcactacc
     2521 agccccagcg tctgagtact cttcgccgac agattgcgcg ccagcaagtt tggacggtaa
     2581 ccgctctctt ctaccgcctg aaacacgcga tctttagtct cctgactgac gtagccatta
     2641 cctgaaagca cgcgggaaac ggtcgctttt gaaaccccgg cgcgcttcgc cacttccagc
     2701 atcgtcgtca tcattttcat ccctttacac gcaatcaacg cagtgtactg caccgtttgc
     2761 cgattgtcct tgcgcaatcg gcgggaaaaa tattcaggtg accggtttca caaatataaa
     2821 aaatgaacaa ttaactctct tgcttattaa atgacaacta ttcatgattt tgtgaaaccg
     2881 gtttcctaat tccgtttcag catcggcatt tttccgtcac gtcgactgat aacaggataa
     2941 aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg tgatagcggc actgggcggc
     3001 gttgataaca tctcggcggt cacgcactgt atgacgcgtt tgcgctttgt tatcaaagat
     3061 gaccagctta tcgacagtcc gacgttgaaa accatctccg gcgtgctcgg cgtggtacgt
     3121 agtgacaacc agtgtcaggt gattataggt aatacggttt cgctggcctt tcaggaagtc
     3181 gtcagcctgc tgccgggaga tatgcagccc gcacagcccg tgggtaaacc caaacttacg
     3241 ctacgtcgca taggtgcggg gatcctcgat gcgctgatcg gcaccatgtc accgctgatc
     3301 ccggcgatta tcggcggatc gatggtcaaa ctgctggcga tgatcctcga gatgagcggc
     3361 gtgctgacaa aaggatcgcc gaccttaacc attctgaatn nnnnnnnnng tgctttctcc
     3421 ttcctgccgc tgatggtcgc tgcttctgcc gccatcaaat ttaaaaccaa tatgtcgctg
     3481 gcgattgcca ttgcgggtgt gctggtacat ccgagtttta ttgagctgat ggcgaaagcg
     3541 gcacagggtg aacatgttga atttgccctg attccggtca ccgcggtgaa atacacctac
     3601 acagtgatcc cggcgctggt catgacctgg tgcctgtcat atatcgaacg ctgggtggac
     3661 agcattacgc cagcggtgac gaaaaacttc ctcaagccga tgctgattgt gttgattgcc
     3721 gcaccgctgg caatcctgct gattggcccg attggtatct ggatcggtag cgccatttcg
     3781 gcgctggttt acaccattca tggttatctg ggctggcttt cagtcgccat tatgggcgcg
     3841 ctgtggcctc tgctggtaat gacagggatg caccgcgtct ttacgccaac catcattcag
     3901 accattgccg aaaccggcaa agaagggatg gtcatgccgt cagagatcgg cgctaacctg
     3961 tcgctgggcg gttcatcact ggcggtggca tggaaaacga aaaacccgga actgcgccag
     4021 acggcgctcg cagcggcggc atcagccatt atggcgggga tttccgaacc ggcgttatac
     4081 ggcgtggcga tccgcctgaa acgtccgctt atcgccagtc ttatcagcgg ttttatttgc
     4141 ggcgcggttg ccggtatggc ggggcttgcc agccactcaa tggcagcgcc ggggctattt
     4201 accagcgtgc agttcttcga tccggcgaat ccaatgagca tcgtctgggt gttcgccgtc
     4261 atggcgctgg cggtcgtgct gtcgttcatc ctcacactgt tgctcggctt tgaggatatt
     4321 cctgttgagg aagcgactgc cgaggcgcga aagcatcaga gcgtacaacc gaccgtcgcc
     4381 aaagaagtaa gtcttaattg aggatgaaaa tgtcagtatt tccagaaggt tttttatggg
     4441 gcggcgcgct tgccgccaac cagtctgaag gtgcgtaccg tgaaggtggc aaagggctga
     4501 ccaccgtcga tatgatccca cacggcgagc atcgaatggc ggtgaaactg ggactggaaa
     4561 aacgttttca gttgcgcgat gacgagtttt atcccagcca tgaagcgacg gatttttatc
     4621 atcgttataa agaagatatc gccctgatgg cagagatggg attcaaggtt ttccgtacct
     4681 caattgcctg gagccgcctc ttcccgcagg gcgatgaact gacgccaaac cagcagggca
     4741 ttgcttttta ccgcgcggta tttgaagagt gtaaaaagta cggtatcgaa ccgctggtca
     4801 cgttatgcca cttcgatgtg ccgatgcatc tggtcaccga atatggctcc tggcgtaacc
     4861 gcaagctggt agagcttttc agccgctacg cccggacctg ctttgaagca tttgatggtc
     4921 tggtgaaata ctggcttacc ttcaatgaaa tcaacattat gttgcatagc ccgttctccg
     4981 gcgcgggtct ggtgtttgaa gaaggggaaa atcaggatca gatgaaatat caggctgcgc
     5041 atcaccagct ggttgccagt gcgctagcca ccaaaatcgc ccatgaggtt aacccgcaaa
     5101 atcaggtggg ttgtatgctg gcgggcggta acttctaccc ttacagctgc aagccggaag
     5161 atgtctgggc ggcgctggag aaagatcggg aaaacctgtt ttttatcgat gtgcaggcgc
     5221 ggggcgcgta tccggcttac tctgcccgcg tattccgcga aaaaggggta accatcgaca
     5281 aagcaccgag cgatgatgag atcctgaaaa acaccgtcga ttttgtctct ttcagctatt
     5341 acgcctcgcg ctgcgcctcg gcggagatga acgccaacta ctgcagtgcg gcga
//
LOCUS       DAWDRC010000241         5392 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6785_length_5392_cov_1.964399, whole genome shotgun sequence.
ACCESSION   DAWDRC010000241 DAWDRC010000000
VERSION     DAWDRC010000241.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6785_length_5392_cov_1.964399"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    533..542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1833..1842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4503..4512
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1274 a         1310 c         1350 g         1428 t
ORIGIN      
        1 gctttgctta cctgcgccag cgagcggttt agttgttgga gggggaaaag tcgcgggtcg
       61 ggaaaggccg aggcaaacgg aactaccgac ggatcacgac tggcctgcaa catatcaaaa
      121 atataggtgt tgatatcgac tgcttcatct cgtgtgactg gaatgactgt cgctttcggc
      181 atttttattg cctgtggcgc aacgtaataa cccgattgcg gtcgcgcgat aatatatccc
      241 tgactttcga gcaactgata ggcatggctg acagtcataa agctcatgcc agaaagcgcc
      301 acctggtcac gcaacgaagg caaacgatcg ccgggttgcc agatacccga cgcaatctgc
      361 tcgcgtaatt gttctgcaag ctgctggtat tttttcatta tcgaatcgta attatgtgca
      421 gatgattcgg cagtctatat cagttgttaa aaatgacaac ttttcattaa ctgtaatcaa
      481 accatcacca gcgataactc tttgccaagc gccttagccg cgagctggac ggnnnnnnnn
      541 nnaattaaat aggcgagtaa tctcctgttt atgtttgcca attcgcctgg ctaactcttg
      601 ctgagtgatt tctgactgta aaaaagcatt taacagcaat accttagagg cgacgctcaa
      661 aggcacttca ataaagtgat cgtgactatt taatggcgaa gtttacctcc gtgtgtaatg
      721 tgctcgaaaa tagcactaaa tcgccgcaac ggcagaattc aatgcaaaat caggaaggaa
      781 gattccagaa tttctttgct ctaatttgca aaactgaata ataacctcaa gaaacgatcg
      841 gcatcatagc ggcaaaaggt tagaaaaagg tggcgggatg ttttcgaagg cgttatcggt
      901 tgtcttatta acgtgtgcac tgttctcagg acaactcatg gcagggcaca aaggacatga
      961 atttatgtgg gtaaagaatg tggatcatca gctgcgtcat gaagcggaca gcgatgaatt
     1021 gcgtgctgtg gcggaagagt cggcggaagg tttgcgcgag catttttact ggcaaaaatc
     1081 gcgcaaacca gaagcgggac agcgttgagt cgtgtgttgc cggatgcggc gtgacacctt
     1141 acccggcagg caatatcatg cagatttgat gagtggtgag taacccgaaa actggcgttt
     1201 tacccttttc gcttcggcaa tgttttcagc agatcatccg gacttacgga tgccactacg
     1261 cttcccggta acggcatttt cagtatgtga tttttgattt tgccaatgac gtgcatctcg
     1321 catgggcggc aatcaaactt tagcgttaat acttcatcgc cgttaaccag ttgcattggc
     1381 gtcgccttcc agcttttgat attgcctttc gcctgtttcg ggcacagatt gaaggcaaag
     1441 cgcagacaat gcttggtgat catcaccggg acttcgccct tctcttcatg tgcctcatac
     1501 gccgcgtcaa tcagttgcac accatagcga tgataaaatt cacgcgcttt ctggttgtat
     1561 acgttcgcga ggaaactcag atgcgtttgc ggataaaccg gcgcaggatc agcaaccggt
     1621 ttacggctgc cgcgctggta actggcaaga cgcgcagcat caagcatatc ggcggcttca
     1681 cggcggaact ggtttaacag actgtttggt acaaacagcg caccaggcag gttaatttgc
     1741 acatcgcggg cgtaatagat ggtttgcccc agttttgcca gaccatcctt cagattgttc
     1801 attgcttttt cggcgttatt ggcttcgtcg aannnnnnnn nnctgccggc cgcccagttc
     1861 aatgtctacc gccacccgac gttcgctgga ggtttttgtc agtgcctgct gccagttatg
     1921 atcaagattt cggtttagtg ggtgatgcgg gcgaattttg tgcaaatctg ctggcatttc
     1981 atttggccag acgcggtact gattttctcc ggttttctct accgtattag cacgaaaacc
     2041 gacgacttca cgtttaatca tcacgttcag gccatcgcca tttgccagtg gctcggtaac
     2101 ggcaacatca atatgatctt tcgctacttt caatacttcg cccaccggca ggccgataaa
     2161 tttcggcgaa tcgaacgcgc caatatcgcc tttacgggca ttcacaaaat aatctgtgct
     2221 accacggtgg aaagtctttt ccgtcgatgg aacaaagaaa tgttcagtac gacctgatga
     2281 agcgcgcgcc agatcgccac gttcttcaat aatggcatcc agcatctgac gataatgggc
     2341 ggtgatattc ttcacgtagc tcatatcttt gtaacgccct tcaatcttga aggagcgtac
     2401 gccagcatca atcagcgcgc cgaggttggc agtctgatcg ttatctttca tcgacagcag
     2461 atgtttttca taggaaacca cccgcccctg atcgtctttc aatgtgtatg gcaaacggca
     2521 cgcctgcgag caatcgccac ggttggcgct acgccctgtt tgcgcatgag aaatgtagca
     2581 ctgacccgaa taggccacgc acaatgcgcc atgaataaag aattcaatgg tcgcgtccgt
     2641 ggcctggtga atcgcgcgga tctgctcaag attcagctct cgcgccagca caatctgcgt
     2701 gaagccaaca tcagagagga acttcgcttt ttctactgta cgaatatcgc actgcgtact
     2761 ggcgtgcagt tcaatcggcg gaatatcaag ttccagaatc cccatatcct gaacaatcag
     2821 cgcatcgaca ccgatctggt agaggtcagt aatcagccgt tgcgcgggtt ccagctcatc
     2881 atcatgcaaa atggtgttaa gcgtgacgaa aatttttgca ccataacgat gggcaaacgg
     2941 caccaactcg gcaatatctt tcaggctatt actggcatta tgacgggcac caaaaccagg
     3001 gccgccgata taaacagcat cggcaccgtg caaaatagct tcgcgggcaa tggcggcatc
     3061 gcgtgccggg cttaacagtt caagacgatg agaagatacg gtcactttct tatccagtag
     3121 ctgaaaaatg gcggctattt tagccacaag cgcgacgttg cgaaatagtt ttcccgctta
     3181 actgcgcggg taatggatga gggaatgaaa atgtacggtt tgctccccac cattacgata
     3241 gacatgcgtg acgtctgcag caaatcgaac gccttcccca caattaaggc tctgccattc
     3301 accatcaaca cacagatcaa gttgtccatc aatgacgacg acatgttcga ttaccccctt
     3361 ttcatggggc gtcgattcgc ttattgcacc gggtgccatc tgaatcgaga agtgttcaaa
     3421 gcagagttgt ggatcataag gaaacagcga agtgatcacc atcgcctgcc gttgtggatc
     3481 ataaactgaa ggcgtagagg actgcggcgg agaaataaat gttgaaaatg gcacgttcaa
     3541 cccggtggca attttccata atgtcgcgac cgtcgggctg gattcattac gctcaatctg
     3601 ccctaacatc gctttagaaa cgcccgtcgc ttctgccagt cgcgaaagac tccagccgcg
     3661 ctgctgacgt agttgtttaa gtgtggtgga taaaaaacga gcgagatttt ccatgcgact
     3721 cctccggcaa aacggaagtt tatcacttgt gcgttataac ggacaaatgc tacgttagcc
     3781 gtacgttata acgcacgagg tgactatgcg tctgttttct attcctccac ccacgctact
     3841 ggcggggttt ctggcggtat taattggcta cgccagttca gcggcaataa tctggcaagc
     3901 agcgattgtc gccggagcca ccactgcaca aatctctggc tggatgacgg cgctggggct
     3961 ggcaatgggc gtcagtacgc tggctctgac attatggtat cgcgtacctg ttctcaccgc
     4021 atggtcaacg cctggtgcgg ctttgttggt caccggatta cagggactaa cacttaacga
     4081 agccatcggc gtttttattg tcaccaacgt gttaatagtc ctctgcggca taacgggact
     4141 ctttgctcgt ctgatgcgca ttattccgca ctcgcttgcg gcggcaatgc ttgccgggat
     4201 tttattacgt tttggtttac aggcgtttgc cagtctggat ggtcaattta cgttgtgtgg
     4261 cagtatgttg ctggtatggc tggcaaccag ggccgttgcg ccgcgctatg cggtaattgc
     4321 cgcgatgatt attgggatcg tgatcgttat cgcgcaaggt gacgttgtca caactgatgt
     4381 tgtctttaaa cccgttctcc ccacttatat tccccctgat ttttcgtttg ctcacagtct
     4441 gagcgttgca ctcccccttt ttctggtgac gatggcatcg caaaacgcac cgggtatcgc
     4501 agnnnnnnnn nnttgattgt gtttactgga ttgctggcac tgtttttttc ccctttcggc
     4561 gtttattccg tcggtattgc ggcaatcacc gcggctattt gccaaagccc ggaagcgcat
     4621 ccggataaag atcaacgttg gctggccgct gccgttgctg gtatttttta tttgatcgca
     4681 ggtctgtttg gtagtgccat taccgggatg atggctgccc tgcccgtaag ttggatccag
     4741 atgctggcag gtcttgcgct gttgagtacc attggcggca gtttgtatca ggcgctgcat
     4801 aatgagcgtg agcgcgacgc ggcggtggtg gcatttctgg taacggcaag tggattgacg
     4861 ctgttcggga ttggttctgc gttttgggga ttaattgccg gaggcgtttg ttacgtggtg
     4921 ttgaatttaa tcgctaacag aaaccgataa taaaaagcgc catctaacga tggcgcgtct
     4981 ggaactactt tttgttaacg tcaaacatgg tggcatcggt cgccatgtca tcaataactt
     5041 gtttaatatt ttcgaaagcc attggcgtac tttcgttatt caggtcttta ccttcgccct
     5101 gacgaacgac tttgataacc ggtttattag tcgctgcatc aatcagctca ccttcaaaat
     5161 acaagcgagt atccatggta cggtggcctg tagccatttg cgtccccgca accactaatg
     5221 caacaggaac cacttcatag aattgcagcc cttctttact ggtatctaca ccggtaatgg
     5281 ccccacggaa aatcagacta cgcggcccag cggtggtaac cagtggttta cgctgcgcta
     5341 tcgcttcttt catttcggtg ttggtatagt tcaaaatttt atccagaact tt
//
LOCUS       DAWDRC010000242         5370 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6811_length_5370_cov_1.950329, whole genome shotgun sequence.
ACCESSION   DAWDRC010000242 DAWDRC010000000
VERSION     DAWDRC010000242.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6811_length_5370_cov_1.950329"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3667..3676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4983..4992
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1346 a         1431 c         1395 g         1178 t
ORIGIN      
        1 cagcagcagg ctggcgagtg cgcctttcgt ttgttgatcg gcgtcgccct gagttaacca
       61 ctgccagcta attaccgcca gcgtcggggg attacctggc ccaagaataa tcgccacatc
      121 cacgaccgac agcgaccagg cgacaatcgc cagcatcgcc attgccagcg cagacgcgac
      181 ggagggcaac agcagccagt tcaggcattg ccagcggctg tagcccagcg aatccagcac
      241 aatgagttgc tgcaacagcc gtttttcgct caatactgcc gctaagatcc acagcagaaa
      301 tgcgctttct ttcaccgcca gggtgaggcc gagcccaata cccaatttgt ccattggcgg
      361 agtgaaatac gggaaatagt cataaagcag ccctccgtca gcaaagagca gaagggcgct
      421 ggtggcaaaa gccacatggg gaatggcgag cagccacggc agacgggcgc acatacgctg
      481 ccatttcggc cctggccaca acgccacaat caccagcagg gcaatcaaca acgccccgac
      541 ggccgcaatg gttgtcgaca ctagcgtcgc cagtaatgcc tgcggtaact gtggatcggc
      601 aaataacgcc tgccagtgcg tcaacgacaa agcaggcgat atcagcgtga gagctgccgg
      661 gatcagcggt gcataaatca ccgccatcat cgcccacagc aaaaagatta atgcgtaccg
      721 taacggtgta gccattcttg ttccagcgca tttacccaac ctgcgtgcgg ttcagccagt
      781 accggcggca gatcctgcgg cattctcgat tgcaatgttt cgcgctgccc gtcaggcagt
      841 ttttgcggat cgagaacaga aggatcgccc cagacagcgg gatctgcttt acgcagttgc
      901 gcatcgggtg agagcaggaa attggcaact accttcgccg cagcactggc attcgcgttg
      961 gcaggaatgg tgacgaaatg gacgttgcca atcatcccct cgcgaaaacc aaaactgtaa
     1021 ctgcttgcag gcaaatcgcc gctggcgatt ttttgctgcg catgtgcagg gttaaaggtc
     1081 agcgacaggc gcaatgtgtt agctttcagc agagcatcca tccgcgcggg cgaaggcggg
     1141 aaatcctttc cttcgcgcca taaatacgga tgcagtgcat caagatattg ccacaagggc
     1201 gcagtgacgc gggcgaaagt cgcatcgtcc ggcgcttctt ttaatgctgt gggatctggc
     1261 gttagcataa tcagcaactg ttcaagaaac gccgtgccgg taaagtccgg tgggcgcgga
     1321 taggtaaccg tgccgggatt ggctttagca aactccagta aggcttgcgg cgtttgtggt
     1381 ggctgtggcg taacatcgcg gcgggcgata aacgtcagtt gtgcgccgcc ccagggcgat
     1441 tcagcccctt ctgtaggcac tgaaaaatct tcccgcaccg gaagctgtgt gtcgacatag
     1501 cgccagttgg gcagagtctc cgcccagccc gtttgtaata aattggcctc ttttaaggtg
     1561 cggaagtttt cgccgttcac ccagagcaga tccaccgaac cgcctgtttt gcgtccggca
     1621 gtagcttcgg tctgaatgcg cttcaccgcg tctgcggcat ccgcgagacg aacaatcttc
     1681 aggtttatag cgtaatgggt tttcatctcg ccgctaaccc agtcgagata gcggttaatt
     1741 gcggtatcgc cgccccaggc gttaaaccag acggtttgtc ctttggcctc attttttatt
     1801 tcctgccagt cactggcatg tgttgccaaa gaaaacagcg ataacaatcc cagcaaccac
     1861 ccacaatggc gcatcttctc tccttaagaa cgtttttcag gaatacggca cagcccctga
     1921 gcgggaatgt ccatggcaga attaaaacgg gctgacagat tttgcaggcc tatcaatgcc
     1981 gtcagttcgg tgagcgcctg agcgtcaaaa tgcgtagcca gtcgggcgcg cagggcatca
     2041 tcgaccgttg gcggcgttac gcttgcggct tcggcgtact ccagcgccag ccgttcttca
     2101 tcgctaaaga gcgggctttg ccgccagtca gccactgcca gcagtttatc ggtgctgccg
     2161 gtacgctcgg cgactttcat actggtgata tccacgcaaa actcacacag gcacatttgc
     2221 gcaatgcgcg cgctgacaag cgatcgtact accggatcga gcggtgagcg tttgcgctcc
     2281 agccagccga caaacatcga caccagataa aagataaacg ggatccgccc ccaccagcga
     2341 atcgggctta gcacctcgcc gtaatggcga cgatgcagcc acgcctgcgg acgtaaaatg
     2401 aaaggaattt tgctaagcgg cggtaaaccc atcaggcatt cctcttctgg cgctcaagcc
     2461 atattttctt cgccataaaa atcaccacgg tcagggcaaa cagaataaac agtccggtga
     2521 caaaccagaa agtaccgcct gttaacatgc tgcccgccca ggaataaaca atagtcgccg
     2581 gtaattgacc aagcccggtg gcgataaaaa acgagcgaaa acgtattgaa gtcaaaccgg
     2641 cagcatagct gattggatcg aaagggacaa aaggcaataa ccgacatacc aggatggtgt
     2701 gtttgccgta gcgagtgaaa aagccgtcca tactgtcaag tacggttttg ccggttaatt
     2761 tttccaccac ttcgcggccc atcactctgg cgataaaaaa gcacagtgcc gcgccggcca
     2821 tcgaactggt ccacgacagt aagccgcccc agaacgcacc aaacagcgac gcattggcaa
     2881 aggtgatcaa aaacgcaggt agcggtgcag caatggcctg taaaatcatc aacaagaacg
     2941 agacaactgc cgctagtgcg ccgtaagact gaataaagcg ttctataccc tgttggtcca
     3001 cggcggcaaa cgccgcaacg ctgcgattga taaactcatg gacgccggga agcagcgccc
     3061 atgccagcag cacagcgaac aacaggatga tgagggttat acggtagtac cagattttac
     3121 gcgattgcat cttcatcatc cgttatcctg ctttattcgg tgattttaaa ggtaaccgtt
     3181 gccagcgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac ttcgccgcgt
     3241 ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg gcagctatcc
     3301 agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc gccaaagcgc
     3361 atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata cgcttttgcc
     3421 gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt cgtttcttta
     3481 ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc ttcataaaat
     3541 gctttcgggg tcgcataacc cataaacagc gatttatgtc cgttggagcc atctttaaac
     3601 acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac cagcatggtt
     3661 accgtannnn nnnnnntggt tattggcatc gacacgcagc ggttggctgt tcgaaagtcc
     3721 gtcgtattcc acttttgcag ccgcattctc tttttgatcg cagccgctaa gcccagcaac
     3781 agccagtaag cagagtgcag ccagtccttt tttccatgac actgaataat gttgcaacat
     3841 cgttctatcc ttcatttttt tggtgagaaa gtgggctgcg gcgagaagca gacagatcca
     3901 catgtttgtg tcgggcgtag agttttgcga gctggacgag aataaacagc gccaggcccg
     3961 ccagacagag ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca
     4021 tcacggtata aataacaata ccgggcaggg tcgtgagtgc tgaaataagg gtataaggcc
     4081 agaaggcgat tgtggttaat ccgtaagcgt aattttgaat attgtaagga aacaacggga
     4141 ttaagcgggt cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct
     4201 gaaaggtatg gctatgacca acgtatttca gcagtaaatc acgtcccagc cagcgcgcca
     4261 tcaggaatga gcacgaggag gccagcgtgg cggcaattaa tgatagtagt gtccctaaga
     4321 gcgggccaaa aactattcct cccgcgatca ccagtatgct tcctggtaac agaaagaggg
     4381 tggcaatgat gaataacaga atatagaggc tatagccgaa caatccgctc tggcgaatga
     4441 gtgtctgtaa gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt
     4501 aaatgaccag cgcaaaaata agacaggcaa aaagaaattt acgctcagcg ttcatcgtgg
     4561 agtttcctat atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg
     4621 gtataacaaa ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg
     4681 ttaattctcc tgacccagtt tgagccagga gatcagctaa attagcggcg gaaggtcgcc
     4741 cagacggggg cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt
     4801 tctacgcaac attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccgcga
     4861 ttatcgtcaa aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga
     4921 ttcgcatggc ggaaggtatc gaccaacccc cagctcatca gcctctccat ccattcgcgt
     4981 tcnnnnnnnn nntcgcgttc ctccggaagg aaagaacatt ttccggtacg cagccagcgc
     5041 ttacggttct cttcgccaat gccaatatcc aggtcgccag gactgatatt catatcgccc
     5101 ataatcagca ccggattttc acgcttgagt tcggtttcca ggtagttttg cagattctga
     5161 taaaactgcg ccttcgccgg gaattttatc ggatggtcgc ggctttcacc ctgcgggaag
     5221 taaccgttga tcacggtgac attacccagc ggcgagggga tttcagccat aataatccgc
     5281 cgttgtgcct cttcgccgtc accgggaaag ccgcggcgca cggcaatcgg cgtctctttg
     5341 gtcagcagcg ccacgccata atggcctttc
//
LOCUS       DAWDRC010000243         5323 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6870_length_5323_cov_2.279043, whole genome shotgun sequence.
ACCESSION   DAWDRC010000243 DAWDRC010000000
VERSION     DAWDRC010000243.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6870_length_5323_cov_2.279043"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    304..313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3865..3874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1279 a         1476 c         1283 g         1265 t
ORIGIN      
        1 gttcttttaa ctcacgcatt gtctgttgcg tggtttcatc atccacggct gcatacccca
       61 gctctttcag ttgccagatt ttgcggcgga acaactcttc cacacgcggc cagttgtttg
      121 gctggctaac atccatcgca ttggataacg tcgcctgagt cgctttgggt gaccactgac
      181 cgtcgtgcag gaaacgtggt acggtatcgt tcacgttggt cgcagcaata aaacgtttta
      241 ccggcagacc gagtgacttc gccagcagac ctgccgttaa atcaccgaag tttccactcg
      301 gtannnnnnn nnnttcttgc ggcaactgtg cgacagcctc aaagtaataa caaatctgcg
      361 ccagcaagcg actgatgttg atggagttag cagaattcag ccccagcgcc actttcagtt
      421 cttcatcatc aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg
      481 caacagtttc gatattgccg cccaatgtac agaacagttt ttcttgcagt ggactgattt
      541 tgcctcgtgg atagaggata accactttca cattcggtaa accgtagaaa gcatgagcca
      601 ctgccgctcc agtatcacca gatgtcgcgg tcagaatggt cactggctta tcgcccgcaa
      661 tatgggtcag catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc
      721 cgtgaaacaa ctccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg
      781 caaacgccgc gcgtacgcgc tcttccagga tttcctgcgg gatttcatca ccaataaacg
      841 ccgagaggat cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag
      901 tcaggctgaa ttccggcagg tcgtgcggga aaaacggccc ctgatttttg cccaacccct
      961 gggttacggc ttgcgcaaag ctgacctgct cattgtgatc tttcagattg tagagtttca
     1021 tttagttttc cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt
     1081 cctgattttg caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct
     1141 tgtcacacag agcgaacaaa gtcgggccgg agccggagat accgctcgct accgcgccga
     1201 tttccgcaac cgcctgccgc gcctgccgga agcctggcag taaccgttca cggtagggtt
     1261 cagcgataac atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt
     1321 gaatgaagcc tgccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg
     1381 gtaaaatagc cctggcttct gccgtcgaga ctttaatccc cggatacgcc agcacccaca
     1441 gccactcatc aaaccctggc acttgctgac tgatgatgtc gttttcttca atcatcaact
     1501 gcataccacc aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgcc
     1561 cttccaactc gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcagtgtt
     1621 cattcatcgc catcagcgcc gcgaccactg aacaggcgct ggagcctaag cccgaaccga
     1681 tcggcatatt cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa
     1741 aacgctccca gcactgataa acgatatttt cccgtggctc tgacggcagc ttatcggcaa
     1801 agcgtccgag gttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga
     1861 gcaatgcacc atcaacaggt gtcaccgccg ccccgagcac atcaaacccg acgctcatat
     1921 tggcactgga agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg
     1981 gtacgtagca gatcagcaaa gacaccggca gctgtaacgt cattgcccgc accatatccg
     2041 cgcagtacca acggcagcgg ctgataatag tggctataga aggccagggc gttttcgcca
     2101 tttttcactt tgaacagcgg atcattacca tccacttcgg caatcttcac gcggcagacg
     2161 ccatcttcat caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc
     2221 acacgcgcgg caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcgacatca
     2281 ccctcggcgt taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt
     2341 tcacgtcccg tttcacgagc gagaatcaat agcttacgcg ccacatccat accagaaaga
     2401 tcatctcgcg ggtccggttc ggtataaccc atttcccgcg ccagtgtggt cgcctcggag
     2461 aaactcatgc cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg
     2521 gagaacttca ccaattcatc acctgcattt aacagatttt gcaggttctc aataaccggt
     2581 aatccagccc caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcatga
     2641 cgcaacagat ggtagtaatc catcgacgag gtgttagcct ttttgttcgg cgtgacgacg
     2701 tggaaacctt cgcgcaggaa gtcagcatac tgatccgcca ctgcctggct ggaagtgcag
     2761 tcaacaatga ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga
     2821 ttaaacggct ctttggcttg cgccagttct tcctgccagt tttccagatt aaggccatgt
     2881 acattggtga gcagagcctt cgagttggca acaccgcaga cacgtaagtc gatatgttta
     2941 ttcttcagcc agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg
     3001 acgccaatca caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg
     3061 cgcacgccag tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat
     3121 ccctgagcaa tggcgacaat gttgatattg gcgcgggcca gtgcggcaaa gaatttcgcc
     3181 gagatcccac gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc
     3241 gtcactgcca gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt
     3301 gcccgttcag ctcgcacaca gtcgctttgt ggaacgcaga aactgatgct gtattcggaa
     3361 gatgattgcg taatcagcac cacggaaata cgggcgcgtg acatcgctgc aaagacgcgc
     3421 gccgccatgc caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta
     3481 ttcagattgg aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc
     3541 gtacctggtg cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg
     3601 atgggggtaa tggtgcgggg gtgaagaact ttagcgccga aataggaaag ctccatcgct
     3661 tcctggtagg acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta
     3721 tagaccccgt caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc
     3781 accgccgcgg agtagtcgga accgttgcgt ccaagcacca ccagttcgcc tttttcatta
     3841 ccggcggtga aacctgccat cagcnnnnnn nnnnagccgg aatacgactt gccgcgatac
     3901 ggcgagtgga ctcagcaata tcgacggtag attcgaggta atgccccact gccagcagtt
     3961 tttcgaccgg atcgataacg gtaacgttgt gaccacgcgc ttctaacacg ccggccataa
     4021 tggcgatcga cattttctcg ccacggcaaa tcagcgcagc gttgatgcta tccgggcact
     4081 gccccaacaa actgatgcca tgcaggacat gttttatttg ggcaaattcc tggtcgacga
     4141 aagctttcaa ttgtgccagc ggaaatcccg gctgggcggc ggcgagtccc gtcagaagtt
     4201 cggcaaaaat acgttcggca tcgctgatat tgggtaaagc atcctggccg ctaatggttt
     4261 tttcaatcat cgctaccaga tggttggtga ttttggcggg ggcagagagg acggtggcca
     4321 cctgcccctg cctggcattg ctttccagaa tatcggcaac ccgcagaaaa cgttctgcat
     4381 ttgccactga tgtaccgccg aacttcaaca ctcgcatggt tgttacctcg ttacctttgg
     4441 tcgaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca
     4501 gcccgcaccg ttacctgtgg taatggtgat ggtggtggta atggtggtgc taatgcgttt
     4561 catggatgtt gtgtactctg taatttttat ctgtctgtgc gctatgccta tattggttaa
     4621 agtatttagt gacctaagtc aataaaattt taatttactc acggcaggta accagttcag
     4681 aagctgctat cagacactct ttttttaatc cacacagaga catattgccc gttgcagtca
     4741 gaatgaaaag ctgaaaatca cttactaagg cgttttttat ttggtgatat ttttttcaat
     4801 atcatgcagc aaacggtgca acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg
     4861 ttgtaaccag tcgaccagtt ttatgtcatc tgccactgcc agagtcgtca gcaatgtcat
     4921 ggctcgttcg cgtaaagctt gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg
     4981 ttgttgtatt aatgttgcta attgatagca atagaccatc actgcctgcc ccagattgag
     5041 cgaaggataa tccgccacca tcggcacacc agtaagaacg tcagccaacg ctaactcttc
     5101 gttagtcagc ccggaatctt cgcgaccaaa caccagagcg gcatggctca tccatgaaga
     5161 tttttcctct aacagcggca ccagttctac tggcgtagcg tagtaatgat atttcgcccg
     5221 actgcgcgca gtggtggcaa cagtgaaatc gacatcgtgt aacgattcag ctagtgtctt
     5281 gaaaacttta atattatcaa taatatcacc agaaccatgt gcg
//
LOCUS       DAWDRC010000244         5322 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6871_length_5322_cov_2.600911, whole genome shotgun sequence.
ACCESSION   DAWDRC010000244 DAWDRC010000000
VERSION     DAWDRC010000244.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6871_length_5322_cov_2.600911"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2449..2458
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3650..3659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4888..4897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1261 a         1388 c         1451 g         1192 t
ORIGIN      
        1 cggtagcaag tcttgctcca acatttggtc gcggtgcgat gaccaaccac tgggtcgaca
       61 tcaagaatgc caacctcgtc gtggtgatgg gcggtaacgc cgctgaagct cacccggtcg
      121 ggttccgctg ggcgatggaa gccaaaattc acaacggcgc gaagctgatt gtgatcgatc
      181 ctcgctttac acgtacggcg gcggtggctg actactatgc ccctatccgt tccggtactg
      241 acattgcttt cctgtcaggc gtattgctgt acctgctgaa caatgaaaaa ttcaaccgcg
      301 aatacaccga agcctatacc aacgccagcc tgatcgtgcg tgaggattac ggctttgaag
      361 atggcctgtt caccggctac gacgcggaaa aacgcaagta cgataagtcc acctggactt
      421 acgaactgga caaaaacggc ttcgccaaac gcgatactac cctgcaacat ccacgttgcg
      481 tgtggaactt gctgaaacag cacgtttccc gctacacgcc agatgtggtt gaaaacatct
      541 gtggtacgcc aaaagacgca ttcctgaaag tctgcgaata catcgctgag accagtgccc
      601 acgataaaac tgcctcgttc ctttacgcgc tcggctggac gcaacactcc attggtgcgc
      661 aaaacattcg tacgatggcg atgatccagt tgctgctcgg caatatgggg atggctggcg
      721 gcggcgttaa cgccctgcgc ggtcactcca atattcaggg gctgaccgac ctggggctac
      781 tgtcgcagag tctgccaggt tacatgacgt tgccaagcga gaagcagacc gatctgcaaa
      841 cttacctcgc tgccaacacg ccgaaaccac tgctggaagg ccaggttaac tactggggca
      901 actacccgaa attcttcgtc tctatgatga aggcattctt tggtgataaa gcgacggcgg
      961 aaaatagctg gggctttgac tggttgccga agtgggataa aggctacgac gtcctgcaat
     1021 acttcgagat gatgaaagag ggcaaggtca atggctatat ctgccagggc tttaaccctg
     1081 ttgcctcatt cccgaacaaa aacaaagtga tcggctgtct gtcgaagctg aaattcctcg
     1141 tgactatcga cccgctgaac actgaaacct ctaacttctg gcagaaccac ggtgaactga
     1201 acgaagttga ctcgtcgaag atccagaccg aagtgttccg tctgccatcg acctgcttcg
     1261 cggaagagaa cggttcaatc gttaactctg gtcgctggtt gcaatggcac tggaaaggtg
     1321 cggacgcccc ggggattgcg ctgactgatg gcgagatcct ctccggtatc ttcctgcgct
     1381 tgcgcaagat gtatgccgaa cagggtggcg cgaacccgga ccaggtgctg aacatgacct
     1441 ggaactacgc cattccgcat gagccatctt cagaagaagt ggcaatggag agcaacggca
     1501 aagcgctggc cgatattacc gacccggcaa ccggtgcggt tatcgtcaag aaaggccaac
     1561 aacttagctc gttcgcccaa ctgcgcgatg acggtacaac ctcctgtggc tgctggattt
     1621 tcgccggtag ctggacgccg gaaggcaacc agatggcgcg tcgtgataat gccgatccgt
     1681 ctggcctcgg taacacgctg ggctgggcat gggcatggcc gcttaaccgc cgcattctgt
     1741 ataaccgcgc ctccgcagat ccgcagggta acccgtggga tccgaagcgt cagttgctga
     1801 aatgggacgg gctgggatat tccggactac agcgcagcgc ctccgggcag cggcgtaggg
     1861 ccgtttatca tgcagcagga aggcatggga cgtctgtttg cccttgataa gatggcggaa
     1921 ggtccgttcc cggaacacta cgagccgttt gaaacgccgc tgggaactaa cccgctgcat
     1981 ccaaacgtta tctcgaaccc ggctgcgcgt atctttaaag acgacgccga agcattgggt
     2041 aaagccgata agttcccgta tgtcggaacc acctatcgtc tgaccgagca cttccactac
     2101 tggaccaaac acgcgctgtt gaatgcgatt ttgcaaccag agcagtttgt ggaaatcggg
     2161 gagtcgctgg cgaataaact tggcattgcc cagggcgata ctgtgaaagt ctcctccaac
     2221 cgtggctata tcaaagccaa agcggtagtg accaaacgta ttcgcacgct gaaagcaaac
     2281 ggcaaagata tcgataccat cggtattccg attcactggg gctatgaagg tgttgcgaaa
     2341 aaaggcttta ttgccaatac gttgacgcca ttcgtcggtg atgcgaacac gcagacgccg
     2401 gagtttaagt ccttccttgt gaatgtggaa aaggtgtaac ggagacgann nnnnnnnngc
     2461 gaaactcatc gacgttacca cctgtatcgg ctgtaaagcc tgtcaggtgg cgtgttcaga
     2521 gtggaacgac attcgcgata ccgtcggcaa taacattggg gtgtacgaca accccaatga
     2581 tttaagtgcc aaatcgtgga cggtgatgcg attctcggaa gtggagcaga acgacaaact
     2641 ggaatggctg atccgcaaag acggctgtat gcactgttcc gatccaggct gcctgaaagc
     2701 gtgcccggcg gaaggggcaa ttattcagta tgccaacggt atcgtcgact tccagtccga
     2761 gcagtgcatc ggctgcggtt attgcattgc gggctgtccg ttcgacattc cgcgcctcaa
     2821 cccggaagac aaccgcgtct acaaatgtac gctgtgcgtt gaccgcgtgg tggttgggca
     2881 agaaccggcc tgcgtgaaga cctgcccaac gggcgcgatt cacttcggta cgaaagagtc
     2941 gatgaaaacg ctggcgagcg agcgcgtggc ggagctgaaa acccgtggtt acgacaatgc
     3001 gggtttgtac gacccggcag gcgtcggtgg tacacatgtt atgtacgtgc tgcaccatgc
     3061 tgacaagcca aatctgtatc atggcttgcc ggagaacccg gaaatcagcg aaaccgtgaa
     3121 attctggaaa ggcatctgga aaccgctcgc agctgttggc tttgcggcta ccttcgcagc
     3181 cagtatcttc cactacgtgg gtgtcggtcc gaaccgtgcg gatgaggaag agaataatct
     3241 gcacgaagag aaagacgagg agcgcaaatg aaacgacgtg acaccatcgt gcgctacacc
     3301 gcgccggaac gtatcaacca ctggatcacc gccttctgct tcatcctggc ggcggtgagc
     3361 gggctgggct ttttgttccc gtccttcaac tggttgatgc aaatcatggg cacaccgcag
     3421 ctggcgcgca ttctgcaccc gtttgtcggc gtggttatgt ttgcctcgtt catcatcatg
     3481 tttttccgtt actggcatca caacctaatc aatcgggatg atatcttttg ggcgaagaat
     3541 attcgtaaga tcgtcgtcaa cgaggaagta ggtgacaccg ggcgttataa cttcggccag
     3601 aaatgcgttt tctgggcggc gattattttc ctggtcctgt tgctggtggn nnnnnnnnng
     3661 cgtgattatc tggcgtcctt attttgcgcc tgctttctca atcccggtga tccgattcgc
     3721 gttaatgctg cattcatttg ccgcagtggc gttaattgtg gttatcatgg tgcatatcta
     3781 cgccgccctt tgggtgaaag gcacgattac cgccatggtg gaaggatggg tgaccagcgc
     3841 ctgggcgaag aaacatcacc cgcgctggta ccgtgaggtc cgcaagacaa cggaaaagaa
     3901 agctgaatga gtattcgcat aatcccgcaa gatgagctgg gttcgagcga gaaacgtacg
     3961 gcggatatga ttccgccgtt attgttccct cggctcaaga atttatacaa ccgccgcgcc
     4021 gagcgtctgc gcgagctggc agaaaataat ccgctgggtg attacctgcg ctttgctgcg
     4081 cttatcgccc acgcccagga agtggtgctg tacgaccatc cgctggagat ggatctgacc
     4141 gcgcgcatta aagaagccag cgcacagggc aagcccccgc tggatattca cgttctgccg
     4201 cgtgataagc actggcaaaa gctgctgatg gcgctaattg ctgagctgaa acctgaaatg
     4261 agcggcccgg cgctggcagt gattgagaat ctggagaagg catcgactca ggagctggaa
     4321 gatatggcca gcgcactgtt tgcctctgat ttctcgtccg tcagcagcga taaagcgccg
     4381 tttatctggg ctgcgctgtc gctctactgg gcgcagatgg ctaatctgat ccccggcaaa
     4441 gcccgcgctg aatacggcga acaacgtcaa tattgcccgg tatgtggctc tatgccggtg
     4501 tccagcatgg tgcaaattgg caccactcag ggtctgcgtt acctgcactg caacctgtgt
     4561 gaaactgaat ggcacgtagt acgcgtaaaa tgcagcaact gtgaacaaag cggcaaactg
     4621 cattactggt cgctggatga cgaacaggcc gcgattaaag ccgaaagctg cgatgactgc
     4681 ggcacttacc tgaagattct ttatcaggaa aaagaaccga aagttgaagc cgtggcagat
     4741 gacctcgcct ctctggtact ggacgcgcga atggagcaag aaggctatgc ccgcagttcc
     4801 atcaacccgt tcctgtttcc gggtgaaggg gagtaatctc tgacgttttc cgggtggcgc
     4861 tggtcgcccg gattacagct catagggnnn nnnnnnngtg tgacgacacc tgctattccc
     4921 gcaaaatctc tcgtatttcg cgaggcgttt tccagatatg atgtaacagt tgcagttatt
     4981 ataagttcaa gtaaaacagc cttgtattaa taccagaata agggcatctg gttttttata
     5041 ttcatgaata aaaaaacttt tgtcattcct tatgctgctt acatagttct tcaacaccaa
     5101 ctgcctcgtc cctttgcctg ccgccttttg ccttacgcac cggaaattgc tgccgctcca
     5161 gatattgctc taccgcttcg cgtaaaattg cagagcgcga aaccttacgc cagcccctga
     5221 gtttattcag ccgtttaatt gcctcttctg ataaatgaag aaaaaccgta ttcatcgcca
     5281 ttaccaccac cttacatata ttactcatta atgtatgttc ga
//
LOCUS       DAWDRC010000245         5303 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6899_length_5303_cov_2.285252, whole genome shotgun sequence.
ACCESSION   DAWDRC010000245 DAWDRC010000000
VERSION     DAWDRC010000245.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6899_length_5303_cov_2.285252"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    248..257
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3035..3044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4485..4494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1155 a         1485 c         1409 g         1224 t
ORIGIN      
        1 aaagcgtcgc ctccggcatg gtgcggtgct acagcttcgc ctcatcaaaa cactggctga
       61 tgatttccag accctgacgg atctgagcgt cttcaatggt gagcggtaca aggatgcgca
      121 gcacgttgta atacgggccg caggagagaa gaatcagacc cttatcgcgg gcacgcgcca
      181 cgatctcggc ggtgagtttt gcgtctggtt tgctgtgatc gccatcttca aacagctcga
      241 tggcaatnnn nnnnnnntcg ggttaccggc ataggtgccg cccagaccgc ctggagcgac
      301 ggcatccatc acttccgcgc gcccggtgac gcccgccagc gggaaaccgc ctgcgatcga
      361 tttcgcaaag gtggtgagat ctggtgccac gcccatttgc tccatcgcaa acagcgtgcc
      421 ggtacgccct gcgccgctct gcacttcatc ggcaatcagc atgatcccgt gctcgtcaca
      481 cagcgcgcgt aaacgctgca taaaggctgg cgtcgcggcg tagaaaccgc cttcgccctg
      541 aacgggctca atcacgatgg cggcgatatc ttccggcgcg gcgtcatttt tgaagatccg
      601 gtggatgctg gcgatagcgt catcctcgct tatgccgtgc agcgggcaag gataaagcgc
      661 gcgataaaca tgacccggca tcagccccat gcccgcagag tatggattca ccttgccggt
      721 cagcgccagt gtgtaatgcg tgcgcccgtg atacgcaccg ctaaaggcga tagtaccgct
      781 acgtttagtg gcggcgcggg cgattttcac cgcattttcc accgcttcag aaccggtcgt
      841 aaccagcaga gttttcttgg caaaatcgcc cggcaccttc tgattcataa tctcgcacag
      901 ctcaagatac ggctcgtaag ccagcacctg gaagcaggtg tgcgacagtt ttttcaactg
      961 cgcttccacc gccgcaacca ctttcggatg caggtgcccg gtattgagca ccgcaatccc
     1021 gcccgcgaaa tcaagatact cacggccttc aacgtcccac acccggcagt tttccgcgcg
     1081 gtcagcgaaa atcgggtgaa tttgcccaac gccacgggga atcgcctgac tgcggcgctg
     1141 cattaactct ttattgctgc tcattcgcat tctccagtta aagaccgatg cacatatatt
     1201 tgatttctaa gtaatcttcg atgccatact tcgaaccttc acgacccaga cccgaggctt
     1261 taatgccgcc aaacggggcc acttcattgg aaataatgcc ggtgttgatg ccgatgatgc
     1321 cgtactccag tgcttcgccc acgcggaaga cgcggcttaa atcacgggcg tagaaatagg
     1381 ctgcaagtcc aaattcggtg tcattggctt gcgcaatcac atcggcttca tctttaaaac
     1441 ggaacagcgg ggcgaggggg ccaaacgtct cttctttcga cactttggcg ttagccggaa
     1501 cgtccaccag aatggtcggc tggaagaagt taccgccgcg ttcatgtgct ttaccgccgc
     1561 aaaccacgcg cgcgcctttc tccagcgcat cggcaatatg ctcttccact tttgctaccg
     1621 ctttttcatc gatcagcggc ccgatggtga cgcctttctc cagcccgttg ccgatgtgca
     1681 gtttgctcac cgcctgctgc aatttttcgg caaagcggtc atacacgccg ttctgcacat
     1741 acaggcggtt ggcgcagacg caggtttgcc cggcgttacg gaatttcgag gccagcgcgc
     1801 cttccacggc tttatcgaga tcggcatcgt caaagacaat aaacggtgcg ttgccgccca
     1861 gctccagcga cacttttttg atgtctttcg cgcactgttc cattaactga cggccaattt
     1921 cggtcgagcc ggtaaacgac agcttgcgca ccagcgggtt gctggtcagt tcgttaccga
     1981 ccgcgcccgc cgaaccggtg actacgttaa acacgccagc cggaacgccc gcgcgaatcg
     2041 ccagttctgc cagcgccagc gcagagaacg gcgtctgact ggcgggcttc agcaccatcg
     2101 tgcagcctgc cgccagcgcc ggaccggctt tacgggtaat catcgccgcc gggaagttcc
     2161 acggcgtgat agccgcggtg acgccaatcg gctgcttgat aacaatcagg cgtttatcgg
     2221 cctgatgacc gggaatggtg tcgccataaa tacgtttgcc ttcttctgca aaccactcaa
     2281 taaatgaggc ggcgtagctg atttcgcctt tcgcttcagc cagtggtttg ccctgttcga
     2341 gggtcatcag gcgcgctaaa tcgtcctgat gctccatcat taaattgaac cagttgcgca
     2401 gaatgttggc gcgttctttg gcagtgagcg cacgccaggc gggcagggcg cggttggcgg
     2461 cgtcgatagc ggcgcgggtt tcatcagcgc ccattttcgg cacgctaccc agcttgtcgc
     2521 cgttcgccgg attggtgacg tcgatgacct cgccattgtt ggcgtccagc cattccccgt
     2581 taatcaacgc ctgctggcgg aataagttac tgtcgttaag tttcatcgct acatcctgtt
     2641 ttcaaaggtt attgattaaa tgcggcgtgt aaggcatcca cactacgtgc cgctcgcagc
     2701 gtgcgtccgg ggttactctg gcttgccaac agcgtttgta ccttgctgac aatatgcgca
     2761 ccaataggaa ttgctgatgt cgctgccggg gagggcgcat tacaggtgtg gatcgtgcgc
     2821 ggggtggtga caaacagaaa atcgtcaatc agcttgccgt ccggcgatac tgcctgcgcc
     2881 cgcacaccgg ggggccaggg ctggagatcg cttaacgaaa gccggggaca atacttttgc
     2941 accagccgca gatagccgct tttgcacagc gagtttttca tctcgcccag tcctgagcgt
     3001 agatggtttt gcagcactcg gcgaatcccc gacgnnnnnn nnnnttccag cgtgtcgcta
     3061 aatgagaagt cgcgcttacg atagccttcg cgtttgaaag ccagcaccgc gtttggcccg
     3121 acggtcacgc tgccgtcgat catgcgggtg agatgcacgc ccaaaaatgg cattgccggg
     3181 tcgggaatgg ggtaaatcag gtggttaaca atctggttat gctccggcgc aaggcggaaa
     3241 tactcgccgc ggaacgggca gataataaag cccggttcga ggccgagcat tttcaccagc
     3301 cggtcagcca tcagcccgga acagctaatc agcgtcgatg cttcatattc accgccctga
     3361 cgggtacgta tcaccacgcc gtttttatgc tcactgaggg cgctgacttc ggcgttatag
     3421 ataatctcac cgcctctggc ctgaaaaatt tttgccattg ccgccgtcac ttcgcggtag
     3481 ctgacaatgc cgctggacgg cacaaaaata ccgcccagcc cggtgatatt tggttcgcgc
     3541 tcgcgcagct cttcggcgtt taaccactcg cgatcgattc cgttcgccgc cgtgcgttcc
     3601 cataaggcgc gcatccgttc catttcgagt tcagacgtgg cgaccagcat cttgccgcag
     3661 ttgtcgtagc gaatgccgtt ttgctcgcaa aaggctttgg tggcgcggtt tcctgccagg
     3721 caaaactgcg ccttcaggct gccgggcgtg taatagaccc cggcatggat tacgccgctg
     3781 ttgtggcccg tctggtgaca ggccgggccg ggctcttttt ccagcaacgc tatgcgggcg
     3841 tcaggataga tatcaatcag ttgcatggcg gtcgacatgc cgatgatgcc gccgccaata
     3901 atcacaaaat catacatccg ctcaattcct ttgcgcttac tgatgcgtct ggtagtggtg
     3961 agtggcgtaa gcgaaatagc cgcgctggcg catcagttca cgacgcagat ccggatgcgc
     4021 ggtaaagcgg tcgcgaccgt gcagccagaa cagattgtta atcaacagga atttgccaac
     4081 gggaacgggt acagaaagaa tgcctttact ggtttcaatg gcatcggaaa gctcgctcaa
     4141 ccacacgcct tcttcgaagt ctttcggctg gacaaactgg tcgatatagc gcatcaccgg
     4201 gcggccctgc tgatcgacat cgaacaccgg atggaagaca tctttgctga cgtttttgct
     4261 cggcggcgcg gcaaagcgca tcggacggcg cgccagcgga tggcggaaaa agaggtccag
     4321 atgttcccag tcatcgagat gcagcagcag cgagttaccg ccctgcatgt tttgctcgtc
     4381 gattttcatc atcagcacgt aatcagtgat ctcttcgacg taagtgccgt cgttgtgcag
     4441 ctccattacg cggtgcggct gacgaagata gctgtctgag ttatnnnnnn nnnntgagtt
     4501 atcaacattt ttcaccacga aacgcgcgta atactgaccg ctcatcgcat cgaaattgga
     4561 acggccaatc agatgcgcca ccgccgtcgc cagcttcacc atctcatccg cctgcgcgac
     4621 atcatcgata cccaccgcat tgatcaacag cgcaccttca gcgcggttta acagggtctt
     4681 caacagcagc ggttgcagct gattcgcaca cagatcgtcg agaattttgc ctacccgaaa
     4741 acgcagaaaa gatttgtact ccagcgcctg tacgggccat tcggcaacct gttcgagaaa
     4801 ctgtttggtc gtctgttcgg tgaaggtgag ttccagcaga cgcggggatt gcgccgacgg
     4861 gatgagagtg aatccgctat agtcctggcc tgaatcgaca gcgttatttt gtacggcggt
     4921 cagtgcattc atcagaagcg atcctcttat gagatgtagg gtgacatggc gatgctcatt
     4981 tcgtagccat aatctaaaaa tatctacatt tctgaaaaat gcgcataaaa gttacatatt
     5041 gttttcttat tgtgatcaaa aacaacaaat taataacaaa taatgtgagg gatataccgc
     5101 agataagcga cgcaagaaat ataaaaaaag aaagtgttta taatcaatca ctttcttttt
     5161 cctcatccat ggtgtattca atgatatcgg taatatcaca tttaaattta ttgcacaggt
     5221 catttagtgt atttaaagtg atacttctgt tatgttttaa attatcaaga gtacctctgc
     5281 tcatatgata gtgttcaatt aaa
//
LOCUS       DAWDRC010000246         5296 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6908_length_5296_cov_2.155123, whole genome shotgun sequence.
ACCESSION   DAWDRC010000246 DAWDRC010000000
VERSION     DAWDRC010000246.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6908_length_5296_cov_2.155123"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4727..4736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1270 a         1466 c         1434 g         1116 t
ORIGIN      
        1 tattaaatca taaaaaagcc cgccaggtgg gcttacctgg cgggcgtgat gatttattca
       61 gcgtttggcg aacgtattag ttccacatgg cgagaatcgg ccagccaacc aacagcagca
      121 tagagatgta aatcaccccg aagattgcgc caagacgcca gtaatctttt gatttcacat
      181 agccacagcc gtaaataatc accccaggac cggttgcata aggtgtcaga cagcccatga
      241 taccgataga cagcaccagc aggatacaca gttgttccat tggtacgccc ggaatccctt
      301 taccgacggc cagaataacc ggcagcatgg ttgcggtgtg cgcagacagg ctggcaaaca
      361 ggtagtgggc aaagtagaac accagaacca gtacaatcac cgttgcgttt ggtgagaagc
      421 cttccaggtg cgtactcatc gtgttggcga accagtcgat aaagccagaa cgggtcaggc
      481 cattcgccat aacaaccagc gttgccaggt tgaccagcgt gttccatgcg ctgttatagc
      541 gagtaatgtc tttccacggc acaacgtgca gcgccagcat cagcgaaact gccagcagac
      601 ctaccgcagt agcattaatg acttcactgc caaataccca caaacctaag ctgagcaata
      661 caaggccaat cagcgtccac tctctgcgcg tcagcgcacc catggttttt agttcatcac
      721 ccgcccaggt tgccacttct tcactgtgtg tgatttccgg tttgtacagc acgtaggaaa
      781 gccacggcgc aatgataagc aagataaccc caaccggcag gaagcagagg aaccactgca
      841 accagctaat ctggataccg gcaattttgc tgacgaactc cagacccagc acgtttggtg
      901 ccgcaccggt gacaaacatg gacgaactca gactggtact aatgaccatc atccacatca
      961 aatagccgcc aatacgacgc gcggacggat cgttcgggaa tgatttaaac agcggcggca
     1021 ggtttttaat gaccggaaaa accgtacccc cggtacgcgc ggtgttggac ggtgtaaacg
     1081 gtgccagcag aatgtcgata atgacaatcg cataacccaa cgtcagcgtg cgtttgccca
     1141 tgaatttcac caggaaaagg gcaatacgac ggcctaaccc ggaaacttca taccctaatg
     1201 caaaaataaa tgcgccaaat accagccata ccgtggtgct ggaaaaacca gccagacccc
     1261 atttcagcgc ctgtttttgc gcattaaacg ctgggtcagc taattctttg gcatcaaaga
     1321 gcaggtaatt actgccaata acgcaaatag taaccgcaat aaaactgatc gccgttgccg
     1381 gaattggctc gaggatcatg ccgacaatca ttgccacaaa cactgcaaag taatgccacg
     1441 cctgcggcgg cataccgtca ggtacaggga taagaaacat gacacccatc accaccaggg
     1501 gcgccaataa tttccatata ttatcttttg ctaaagacat acgggttctc cgaaaattaa
     1561 tatttccaaa tttatcaagt gcttaaataa ttaaatctgt gctaaaaacc aggtaaggat
     1621 cagcaggtcg gcactgccgc ctggactgag atttcgttcg atacactccc tgtcgaactg
     1681 ccggagataa tcgagatcgg cgggggttcg gatgccccca ttgtttaata atgtttgcgc
     1741 ctcgcgctgt agccagcgca ggcccccctc gccaccgcgc gatgcaacgt tggtatcgcc
     1801 gttggtagcc atcagtagga gcaaggtatc gagcaatgcc agctcaggat ctaacccctg
     1861 atccagcaga gtgaggtaat gcggcaaggc gtggttgatc accagtggat aacccgcttc
     1921 ggcttcaccg cgtgcgccgg taaggccaag ctgctggtac aaccgttgac ctgccgtcag
     1981 tcgtgaatta ttggtacgca gttcgcgatc ggtcaggcca cggcagaaac ttgccgccgt
     2041 agaacaaacg gttgttggcg ttaccggctg gttgagctga agcaaacggc caattgccgc
     2101 acacagtagc cctaaagaaa aaatgcttcc tttatgcgtg tttacgcccg cagtggcgcg
     2161 gaacatatca ccttcgcaag ccataccaat tgggcgtaat ccgttgagta ccgcttccgg
     2221 tgccatttct gcactacagg caccaaattc aatgaaacgg ggtaaccagc cctgaatcgc
     2281 cagcgcgctg cggtggaaat cttccagcgc catatctttg tgcgcaccgc agttaatgcg
     2341 atccacgagg cctggtttcg gtgacaggtt gacttcagtc agcatggcgc gccagcccag
     2401 cagggcgtac tcatcgatta atgacgtcgc aagctttgtg gttttagttg acgttgcagg
     2461 catcgacatc gttcagcagc gcctccatgc ggttgagtaa atcggtcagt tgatgggttt
     2521 ttccacgcgc gcaaacagct gcgctttgct cgcacaacag gcagcggcga ggcggcagtg
     2581 aataatcgcg gcgggagaga atgtcgcctt cgggcgtcag gacatcgata tcccataacc
     2641 gcccgagagg atgactatgt tcaagctcaa tggtggcgag cttgaggtcg cgagccgggg
     2701 cggcaatgct caacatgccc tccggcccgc tggcggaaac cagtgcagcc tgctcctgaa
     2761 tttgccagcc ctgttttgcg gctaaggcac gcaaggctgt cacgccatga ttaaaaattc
     2821 ggcgtgtgac ctcgctgtct ttaatcggcc caggcgcaac cacggtaaag gagaccagtg
     2881 gaacaggatg gcgcttgagc caggcgtgtt gccgtgcttg cctttcatcc cggctgacga
     2941 gcagctcggg aattgacacc gcatggtggc tggcgagttc aggaagcagg tgcatggctt
     3001 attccttcac ctgatgcaca acatcgatca ccgagccatc gcggtaacgc acaacggcaa
     3061 cgacgcggtc ggtgaattca atcggctgtg gttcaccggt cagcagacgc gcacgttcac
     3121 gcagccactc aatagaaacc actttgatcc ccgcttcctg cagacgttct gccagctccg
     3181 gacgtgccgg gttaacagcg atgccgtggt ctgtgaccag aatatcgaca ctggagcccg
     3241 gggtgatgca ggtcagcacg ttatccacca gcgtcggaat acgaccgcgt accagcggcg
     3301 cgacgatgat ggaaagcgca gaagcaatcg cggtatcgca gtgaccaccg gaagcaccac
     3361 gcagtacgcc gtcagagccg gtcagcacgt taacgttgaa ctgggtgtca atttccagcg
     3421 cgctcagtac caccacgtcg agacgatcaa ccgctgcgcc tttcgacccc cagttagcgt
     3481 actggttggc gctgatttcg atgtgattgg ggttacgggc cagcgattgc gcagcatggc
     3541 tgtcaaagct ctgcacatcc agcagtttgc ggatcaaacc tttttcgtgc agatcaacca
     3601 tcgttgcggt aataccgccg agggcgaagt cggcgcgaat atcgcggcta cgcattttgt
     3661 cttccaggaa acgggttacc gccagcgaag cgccgccggt gccggtctgc atggagaaac
     3721 cttctttgaa gtagccagag ttaacaatca catccgcagc gctacgggca ataagcagtt
     3781 cgcgcgggtt agtggtcata cgggtagcac cagcgccgat ttttgcagca tcgccaacgc
     3841 gatcaacttt gacgatcaga tcaacctgat cttgctcaat gcttgccgga ttatgcggat
     3901 aaggcagcag ttcttcggta agcatcacga cctgttttgc gttgtcggca tcaaccattg
     3961 catagccgag ggagccgcag caggctttac cggagtagcc gttggcatta ccgaattcat
     4021 cacaggacgg gacgccgagg aaagccacat cgatattcag ttcgccgctc tgtaccagat
     4081 gcacacgtcc gccgtgagag tggatttgca ccggttccgc cagcagacca cgggagattt
     4141 cttccgccag tggaccgcgc aggccagagg tataaatgcg ggtaaccacg ccctggcgaa
     4201 tgtgttctac cagtggcgca tggcaatcac tcagggagct ggaggccaga gtcaggtttt
     4261 taaagcccat cttcgcgatg acgtccatca ccatattgac ggtcaggtca ccgccacgga
     4321 aagcgtgatg gaaggaaacc gtcatgccgt cctgtaaacc agagcgacga atcgcttctt
     4381 ccaggttggc gcacagtttg cgatcgcgcg ctttttcagc ctggtaggtt tgctttggcg
     4441 agttctggaa agcggcaaga tcgcattcag cgcgacgatt ccaggccgct acccgttctt
     4501 gtcgttgaga ttgttcaatt ttctgcgtca ttttgattgc cttattcttc gcggatgccg
     4561 gaaagttctg cgcgggagag caccagacgg gcgcgatcga taaccggacc gtccaccatc
     4621 ttgccgttca gggaaaccac gccgaggcct tcgcgagcgg cggcttcagc ggcttctacg
     4681 acgcggcggg cgtgatccac ttctttctgg gtcggtgcgt agaggtnnnn nnnnnntgtg
     4741 cagcagatca atctgacgcg ggttgatcag cgatttgccg tcaaagccca gctgtttgat
     4801 gtgggcggct tcttgcagga atccggcttc gttgttagcg tcggaataga cggtatcgaa
     4861 cgcctgaata cccgcagaac gcgcggcctg caaaatggag cagcgagcga acagcagttc
     4921 agttccttcc ggggagcgtt ctgtacgcag gttgcgcaca tagtcttctg caccgagggc
     4981 gataccgatc aaacgctcgg aagcgtgagc gatttccact gcgcgggtaa tacccagcgg
     5041 agattcaatc gccgccagca ggccggtact gccgggttca cgaccacagg ctttttcgat
     5101 tcgcaggatc tctttttcga tatccagaac atcctgagcg gtatcggttt tcggcagacg
     5161 cacaacgtcc gcaccaccgc gaacgacggc ttccaggtcg ttaacacccc attcggaatc
     5221 cagcgcgttt acacgcacaa tggtttcaat atcgcgataa agcggatgtt gcagcgcgtg
     5281 gtaaaccatg cggcgg
//
LOCUS       DAWDRC010000247         5294 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6911_length_5294_cov_2.423936, whole genome shotgun sequence.
ACCESSION   DAWDRC010000247 DAWDRC010000000
VERSION     DAWDRC010000247.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6911_length_5294_cov_2.423936"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    220..229
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1067..1076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1246 a         1396 c         1454 g         1178 t
ORIGIN      
        1 taaagcggcc aacgtaggcg cggcttggca tctcggtgtt gccgatcttc atgatatcca
       61 gcccgccgga aacttcttcc caaacggttt tgctgttatc cattgagtca cggaactgat
      121 caaccgacgc cagtttcacc gtttcaccca aagtgatggt gccgctgtcc ggctgttcct
      181 gaccagagat catacggaac agcgtcgatt tacccgcacn nnnnnnnnng ttcggaccga
      241 tgatcccgac gatcgctcct ttcgggatcg agaagctcag ggagtcaatc agcagacgat
      301 caccatagga tttacgcagg ttgctgactt ccagcacttt atcgcccaga cgcggtccag
      361 gtggaataaa cagttcgttg gtttcgttac gtttctgata ttcggtgctg ttgagttctt
      421 caaagcgcgc cagacgtgct ttaccttttg actgacggcc tttagttccc tgacgtaccc
      481 actccagctc tttctcgatc gacttacgac gcgccgcttc ttgtgaagct tcctgcgcca
      541 gacgctgatc tttctgctcc agccaggagg agtagttacc ttcccacgga ataccttcac
      601 cgcggtcaag ttcgaggatc cagcccgcaa cgttatcgag gaagtaacgg tcgtgggtaa
      661 tcgccaccac ggtgccttcg aagtcgtgca ggaagcgttc cagccacgct acggattccg
      721 catccaggtg gttggtcggt tcgtcgagca gcagcatgtc tggtttttcc agcagcaggc
      781 ggcacaacgc cacgcgacga cgctcaccac cggaaaggtt agcgattttc gcgtcccagt
      841 ccggcagacg cagcgcatcc gccgcacgct ccagctgaac gttcaggttg tgaccgtcgt
      901 gagcctgaat gatctcttcc agacggcctt gttcagcggc cagcttgtca aaatcggcat
      961 ccggatcggc atacagcgca tacacttcat ccaggcgttt cagggcgtta accacttcag
     1021 aaaccgcttc ttcaatggac tcacgaacgg tgtgttccgg gttcagnnnn nnnnnnaatc
     1081 ttgatgtctg gctgcggacg cgcttcacct tcgatgtctt tatcaatgcc cgccataatg
     1141 cgcagcaggg tggacttacc cgcgccgttc agacccagga caccaatttt tgccccaggg
     1201 aagaaactca gagagatgtt tttcaaaata tgacgtttcg gcggaacaac tttgccgaca
     1261 cgatgcatgg tataaacgaa ttgagccacg ttggacttcg cctctatgtt tatcgtgatg
     1321 atgagttttc aaaggcgaag tgtagcctgt ttccctgtct aatcccagcc agtcgatcac
     1381 cccgtaaaag taaaaaagtg tccgtaacgt ggcgtaaacg gcaatgactg gttagcataa
     1441 atctattacg cggcatgacg ctgcattgat gtatttacac ttagaggatg cgcttgtgga
     1501 aaaagccaaa cacgttacct ggcggctgtt ggctgtcggt gtctgtctgc tgacggtcag
     1561 cagcgtggcg cgagccgact cactggatga gcagcgtagc cgttacgcgc aaattaagca
     1621 ggcctgggat aatcgacaaa tggatgtgat cgaacaaatg atgcctggac tgaaagatta
     1681 cccactttat ccctacctgg aataccgcca gatcactgat gacctgatga atcaaccggc
     1741 ggtgacggtc actaactttg ttcgcgctaa ccccacgctt cctcccgctc gcacgctgca
     1801 atctcgtttc gtcaatgaac tggcgcggcg tgaagactgg cgtggcttgt tagcctttag
     1861 cccggaaaag cccggaacta ccgaagcgca atgtaattac tactatgcga aatggaacac
     1921 cgggcagagt gaagaagcct ggcaaggggc gaaagagctg tggctaaccg gcaagagcca
     1981 gcctaacgcc tgtgacaagt tatttagcgt ctggcgtgcg tcaggtaaac aagatccgct
     2041 ggcgtattta gagcgtatcc gtctggcgat gaaagcgggt aacaccggcc tggtaacagt
     2101 gctggcaggg cagatgcctg ccgattacca gactatcgct tcggcaatca tttcactggc
     2161 gaacaaccct aatacggtac tgaccttcgc gcgtacaacc ggcgcgaccg attttactcg
     2221 tcaaatggcg gcggtggcgt ttgccagtgt ggcgcggcag gatgctgaaa atgcacggct
     2281 gatgatcccg tcgcttgctc aggcacagca gcttaatgaa gatcagattc aggagctgcg
     2341 cgatattgtc gcctggcgtt tgatgggcaa cgatgtcacc gacgagcagg cgaaatggcg
     2401 cgatgacgcg attatgcgct cgcaatctac ttcgcttatt gaacgccgtg tgcgaatggc
     2461 gcttggcacc ggcgatcgtc gcggcctgaa tacctggctg gcgcgtctgc ctatggaagc
     2521 gaaagagaaa gatgaatggc gttactggca ggcggattta ttgctggaac gcggacgtga
     2581 agctgaagca aaagagattt tgcatcaact catgcaacag cgtggtttct acccgatggt
     2641 tgctgcacaa cgcatcggcg aagagtatga gctgaagatt gataaagcgc cgcagaatgt
     2701 tgacagcgcc ctgactcagg ggccggagat ggcgcgcgtg cgcgagttga tgtactggaa
     2761 tctcgataac accgcgcgta gcgagtgggc caatctgatg aagagcaagt caaaaacaga
     2821 gcaggctcaa ctggctcggt atgctttcaa taaccaatgg tgggatctta gcgttcaggc
     2881 aacgatcgcc gggaagctgt gggatcatct ggaagagcga ttcccgctgg cttacaacga
     2941 tcttttcaaa cgctacacca gtggtaagga gatcccgcaa agctatgcga tggcgattgc
     3001 ccgtcaggag agtgcctgga atccgaaagt gaaatcaccg gtaggggcca gcggcctgat
     3061 gcagattatg cctggtaccg cgacccatac ggtgaagatg ttctctattc ccggttatag
     3121 cagccctggg caattgctgg atccggaaac gaacatcaac attggcacca gttacctgca
     3181 atatgtttat cagcagtttg gcaataaccg tattttctcc tcagcagctt ataacgccgg
     3241 accagggcgg gtgcgaacct ggcttggcaa cagcgcaggg cgtatcgacg cagtggcatt
     3301 tgtcgagagt attccattct ccgagacgcg cggttatgtg aaaaacgtgc tggcttatga
     3361 cgcttactac cgctatttca tgggggataa accgacgttg atgagcgcca cggaatgggg
     3421 acgtcgttac tgatccacac gtttatgata tgctatcgta ctctttagcg agtacaaccg
     3481 ggggaggcat tttgcttccc ccgctaacaa tggcgacata ctatggccca acaatcaccc
     3541 tattcagcag cgatggcaga acagcgtcac caggagtggt tacgttttgt cgacctgctt
     3601 aagaatgcct accaaaacga tctccattta ccgttgttaa acctgatgct gacgccagat
     3661 gagcgcgaag cgttggggac tcgcgtgcgt attgtcgaag agctgttgcg cggcgaaatg
     3721 agccagcgtg agttaaaaaa tgaactcggc gcgggcatcg cgacgattac gcgtggatct
     3781 aatagtctga aagccgcgcc cgtcgagctg cgccagtggc tggaagacgt gttgctgaaa
     3841 agcgattgat tttgtaggcc tgataagaca tggcgcatca ggcatcgtgc accgaatgcc
     3901 ggatgcggcg tgaacgccta atcaggcctg caatggctga tacacggcat tatgaaacgg
     3961 actcagcgcc aggatcaccg cctggtgata gacgctggtg cgagtgagtt tcccggcggt
     4021 aaacacgccg atcgcccctt ccttacgacc aatctcatca ataccggtat aacgcgacat
     4081 cacgggacca agcgcctcac cttcacgcac tttttccaga atcaccgcag gcaacggcaa
     4141 agtagccgaa cgcgcctcgc cgcgctggct ggcgttttca atcaccaccc aactgaaagt
     4201 gctgtcacca tcgatgccag cttcaatcgc cacccaaaaa tcagcctcag gaagtaaacg
     4261 gcgggcattg gctaccctat ttcgtgcgcc agcgcgcgtt tcctcactgc caaagggctg
     4321 ttccggtaca ccgctctcga cggcaacgga tgcaatatgg caggatcctt cgccgaagat
     4381 ctcgtgaaat gcctgcagaa tggcctgaat tttagcggga ttggtggtcg cacagacaac
     4441 ttggtgcata atcagcatta ctcagaaaat taacgttaca gcagtatacg gaaaaaaagc
     4501 atgttacagg tatacctagt ccgccacggt gaaacgcagt ggaacgccga gcgacgtatt
     4561 cagggccagt ctgacagccc gctgaccgcc aaaggtgagc aacaggcgat gcaggtggca
     4621 acccgtgcca aagagcttgg cattacgcat atcatcagta gcgatttagg acgcacccgg
     4681 cgcacggcgg aaatcatcgc ccaggcctgc ggttgtgaca tcatctttga ttctcgcctg
     4741 cgtgaattaa atatgggtgt actggaaaca agaaatatcg attcactcac cgaagaagaa
     4801 gagaactggc gtcggcaact ggtcaatggc accgttgacg ggcgtattcc tgaaggtgag
     4861 tcaatgcagg agctcagcga tcgcgttaat gcggcactgg aatcttgccg ggacttaccg
     4921 cagggaagcc gaccgctact ggtgagccat ggtattgcac tgggatgcct ggtgagtacg
     4981 attctcggat taccagcatg ggcagagcgc cgcttacgtc tgcgtaactg ttctatttcg
     5041 cgcgtggatt atcaggaaag cttgtggctg gcgtctggct gggtggttga aaccgcaggg
     5101 gacatctcgc atctggacgc ccctgcatta gatgagctgc agcgttaacg acggctcgga
     5161 atcagcagtt cacagcgcag attaattggg cgatctcccg ctttggcatc ttctgccggg
     5221 tagtatcgct caatatcctg acctttacgg cgcgtcaagt taagcattgg catgcacgtt
     5281 ccgtataccg tcag
//
LOCUS       DAWDRC010000248         5274 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6940_length_5274_cov_2.347001, whole genome shotgun sequence.
ACCESSION   DAWDRC010000248 DAWDRC010000000
VERSION     DAWDRC010000248.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6940_length_5274_cov_2.347001"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1227 a         1348 c         1420 g         1279 t
ORIGIN      
        1 atctgcaaat gacttggctg ccacagccct tcgtactcct gcttatggtt gtcgatcatc
       61 ggcttgaaga agttgatctt tggctcgcgg gtcagcagcg actcgccatt cacttgccag
      121 gatgtcggtt taccactcgt ttttgagaag gtgatcgcaa agttgtagcc gcgaacagta
      181 cagctcaaac gatcgtcttc cagcgtcagc ggacgggcgt tattcggtgc gaaaggtaca
      241 ggctgcgcgg tgttttcctt cagcgggaac tgataagtgg cgatagaatg cccggcttcg
      301 ctgtagcggg tgcgggaatc tttggtcacc gtaatgttga ggaacgcttc gcgggcgtcc
      361 agctgcggca gcgtgatctg taagggggct tcgctgttcg gcgcaacgtc gcgcagttta
      421 atctgttgcg tcgcgagcgt ttcaccttcg gcgcgcacct ctgcgtgcag ggtgtagtca
      481 tcaagcgtgg taaaccatag tttattttcg actttcaact cgccgcgagt cagatccagc
      541 gcgtggattt ttaccggcgc aataacctgt ttgtactctt tcaggcctgg tcccggcgtc
      601 tgatcggaat agatcaaacc atcaagacag aagttatagt tgttgggata gtcgccgtag
      661 tcgccgccga atttgtacca gacgttgccg ttgtcatcct gcgcctggat cccgtggtcg
      721 caccattccc agacataatg tccctgaatg caatcgtgct tatagaagac gttctggtac
      781 tccgtcagcc cgcccggtcc gttccccatc gcatgagcat attcacagat gatgcgcggc
      841 ttcggatgcg ggtattcacc aaactcattc atcagaggca cgcgggtgta catggtggaa
      901 ataatatcga ccacttcagc atcgcgatct tcttcgtaat gcaccagtcg cgtgtcatcc
      961 agcgccttcg ccgcatggta catcgcgcgg atgttacagc catagccgga ttcattgccc
     1021 agcgaccaga tgatgatcga cggatggttt ttctgcgcgt gaatatggcg aacaatgcgc
     1081 tcgacgtaaa ctttttccca ctgcggatcg tcggtaatgc ggctgatatc gccgacgtta
     1141 gcaaagccgt gcgattcgac gtcggtttcc gccatcacaa acaggccata gatatcacac
     1201 agttcgtaaa aacgtggatc gttcgggtag tgagcggtac gcacggagtt gatgttgtgc
     1261 tgcttcatca actggagatc tttctcgacg cgatccatcc caacggcgcg gcctttgcga
     1321 tgatcgttgt cgtggcggtt gacgccgtgc agcatcacat aacggttatt gatccagaac
     1381 agaccgtcgc gcactttgat atcacggaag ccaacgcgtt gtggcaccac ttccagaacg
     1441 ttgccgtcgg cgtctttcag catcatgacc agatggtaaa gataagggga ttctgctgac
     1501 cattgctgcg gctgttcgac agtaaaagca aagcgggcgc tggtcagttt ttcaattgcc
     1561 agatgatcaa tggcgctgct gtgcaccacg tgttcgccat caaacagggt atattccagc
     1621 gtcgtgacga caggggaggc ggcgagattt tccagcacca cttcgcagga aagtgtggca
     1681 tcgcaatagg cttcgtcaaa gtcggtacga acggtgaaat cgttaatatg cgtgagttgt
     1741 tttccgatca gataaacatc gcggaaaatc cccgccgacc accacatatc ctggtcttcc
     1801 acgtaggtag agtccgccca ttgcatcacg cgcacacaca acaggttgtc gccggtttta
     1861 accatcgcgc tgatgtcaaa ctctgcggtc aggcgactgc ccttgctgaa ccccacatac
     1921 tgaccgttaa cgtagacttc aaaataggtt tcgacgccgt caaatttaat cagcgtctgt
     1981 ttaccctgcc agccgtcgct gagggtgaaa atacgttgat aggcaccggt tgggttatcg
     2041 ctggggacaa acggcacatc gatggggaac ggaaaacctt cgtcggtata ttgcagtttg
     2101 ccgtgacctt ccatttgcca catggcgggg acggtaatat gcccccagtc agccattaac
     2161 tcagaggtga aggcttctgg cacttgcagc ggatggtcaa aaaagtggaa attccactga
     2221 ccgcttaagg acagaaacag gctgctggtt tcgcgggcaa aagtacgagc ctgcgcaaca
     2281 gaatcatatg aaaaaaagta cgcacgcggc gcaagtcggt tttcgtgggt gagctgaatg
     2341 ttttcccagc gattcatagg gcctcccggt agagagaaat cggaaccatt catggtgatg
     2401 gtagtttgtg taattgctgg ggagtttagt aaaaatttta ctaaaaagta gcggataaat
     2461 gcatttactt taactggatc acgaaaataa acggcagcac tgtccggcaa aaacgattgc
     2521 cggacagtgt gtaaggggat ttaacgggtc gtgccgcgca gttttaattt gctgggaacg
     2581 aagaccaaca gcggcagcgc gcgaccatcg cgggcttttt catacaccag gttaacgccc
     2641 tgacttccca tcatctcgga atggatgcgc acggtggaga gcggcggaaa ggtaaatcgc
     2701 gcggtgggga tatcgttaac gctgataagc gaaatatcct gtgagatgtt caggccacgt
     2761 tcatgaattg cccgcagtac gccgatagca atggaatcgg aagcaacaaa cagtgccttc
     2821 ggatagtctt cccgcgccag catctgtttt gccagttcat agcccgacga actggaaaaa
     2881 ccgccgcgcc agatatcctc ttcgcgtacc acgtgtttca gacgaccata ttccgcaaag
     2941 gcgacctcac gaatatccgc cttgccaggc aagtcttcac cgccaataaa accaatacga
     3001 ttaacgccct gattgatata gaaatcgatg atttctttac tgatacgtgc cagatcgata
     3061 tccaccgcat cgtaaccgct gccgggttcg tgaaagtcga taaaacagat attgtcggtc
     3121 aacgcacagg cggcggcgcg cagggcggac gtgggtttgc cgacaattaa aataccggtg
     3181 acgtttttaa tgtctggtaa gccgttgtgt tcataacagt tggtcagttc gatcgccagc
     3241 ttttcgcact gagtttcaat gccgtggcgg atcgccagat agtaaggatc gttgatctcc
     3301 agctcctgtt ggtagctgta gatagccaga atatggtgtt ggttgactgc gcctgtctgg
     3361 agtttacggg cgctactggt cttgtactcc agcttttcgg cgatctcgag aatgcgatgt
     3421 ttcgtctctt ctttcacatt caatgtcgga tcgtcattca agcccctgga tactgtcgcc
     3481 agggatacgc cagcttcgat tgcgatgtct tttagtgttg ccatgtttac cttttttact
     3541 ttccctgatg tgcgccccca ttgtagagga agtgcttatc gggtcacctg ttttcagtaa
     3601 ataaagcgcg tttctttgac gctgtaacgg taacacagcg gcttgcgaat gtgatcacgc
     3661 ctgcattctc catttttagc ctacaggatt gagaaaaact tttgctatag tttagtaaaa
     3721 ttttactcga agggaggaaa caatggaaac cgtggcttac gctgattttg cacgtctgga
     3781 aatgcgcgtc ggaaagattg tggaagtgaa acgccatgaa aacgccgaca agctgtacat
     3841 cgtacaggtt gatgtggggg aaaaaacgct gcaaaccgtg accagtctgg tgccgtacta
     3901 cagcgaagaa gagctgatgg gaaaaacagt ggtggtctta tgcaatctgc aaaaagcgaa
     3961 aatgcgcggt gaaacgtcgg aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga
     4021 aagcgtgctg ttaacgccgg agcggatgat gcctgcgggc gtacgtatcg tataaaaaga
     4081 tcggatggcg acgtcgtatc gccatccgat ttgatattac gcttcttcga cactgactcg
     4141 catggccgcc agcgccttac gcgccgcttt gatcaccagt tcacactgtt caatggtcag
     4201 tgtcagtggc ggttcaatgc ggatcgtttt agcgttgttg agtgttccgg ccaccagtac
     4261 gcgctggcgg aacatctcgc tggcaaagtt atagccgatt tcgttatcaa caaactcaat
     4321 cgccatcaac atccctttac cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag
     4381 ttgacgaaaa ccgtccagca acatatcgcc tttttgttca gcctgagccg gtaagttctg
     4441 ctccagcaac acattgatgg tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa
     4501 ggtggtggta tgcaggaacg gattgtcgaa caggactgag aacacctctt cagtggcgat
     4561 agttgcgcca atcggcatca cgccgccgcc gagggctttt gccagacaaa ggatatccgg
     4621 ctgtacgttc tcatgctcgc aggcgaacat cttgcccgtg cgccccatgc ccgtttgtac
     4681 ttcatcgagg atcatcagtg cgccgaactc atcgcacagc ttacgcacgg cggtgagata
     4741 gcctggcggc ggcagaatga cgccaccttc gccctgaatc ggttcgagga tcaccgcagc
     4801 aacatcatca ccggtttttt tgcactcgtt aagagccgtg cgcatggctt cgatattgcc
     4861 aaacggtaca tgacgaaacc ctggcagcag tggcataaaa ggtttgcgga aggtcgattt
     4921 cgccgtggcc gacagcgcgc caagtgattt accgtggaac gcgccgctgg tggcaataaa
     4981 agtaaacttg ccgcgcggtg actgataagc cttcgccagc ttcagcgccg cttcaacgga
     5041 ttcggtgccg ctattacaga agaagctgta tttcagttta ccgggcgtta gcgccgcaag
     5101 ggttttcgcc agcatcgccc gtaacggatc gagcagctcc tggctgtgaa gcggttgttt
     5161 agcaagttga ttctgtacgg cggaaaccac aactggatta cggtgcccca cgttgaaaat
     5221 tccaaaacct cccaggcagt cgataaactc ctgtccctgg gtgtcgacaa gcgt
//
LOCUS       DAWDRC010000249         5273 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6941_length_5273_cov_2.297049, whole genome shotgun sequence.
ACCESSION   DAWDRC010000249 DAWDRC010000000
VERSION     DAWDRC010000249.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6941_length_5273_cov_2.297049"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1343 a         1372 c         1243 g         1315 t
ORIGIN      
        1 ctcgctgaag gcacgcacaa caacgtttcc ggcacgggtc gaagaaaccg tggcgctgta
       61 aacccctggt tttatttccg aaatggcacc aacggttaca ccattagtgt cttgcggaac
      121 aagtcgcagg cggctggctt ctcccgtcac cggattaccc tcggagtcca ccgctgtcag
      181 tgtcagcgtg taggcttgct gaccatcagc aaccacatca cctgacggct cgttagcgct
      241 cagggtggag tttgccacat ccatcatcgt ggcccgcagt tctgcagtga cagttttgct
      301 catgtcatca acgctaacag taatcgttgc ctcacctgac tgcgttccgg tagtaaagac
      361 agactgatac acccctgctt cagtttcggt gaactcaccc agtgttggct ttgcctgtga
      421 tttagtggcc ttcagggtac gagtcacaat atttccagcc ggtttgaagg ttagttcagt
      481 cttgatctga tctttcatgc ccgtgactgg ctggccctcg gcgtcgcgca gagacagcac
      541 cagcggcttt tgctcattac cgttagcaag catttgaata cggctctgac cgtcaagcgt
      601 taacgccgta cgatcggcgc tcataccagc tccgctaata accacttctg tctgcacacg
      661 tttcgaggca ttgcctttgt tatcgtaggc aatcgctgaa atcgcataat aattgtcttt
      721 gcctgcctga taagccggga gcgtcacttg ccactgattg ccctgccccg taatttttcc
      781 gcctgcggcc agcaaagacg gcgcttccca ttgcacattt ttcagaccgt gagttgcttt
      841 gctgaccaca agccccaggg aaaccgtctg gccgcccttg ccttcaatac gctcaggcag
      901 agcaatacgg atcacttcag atttgcgata ctcaagaacg atattgttat tacgctcaac
      961 caggtcatag cggctgcctg ccagcattcg acgctcgcga atgctgtctg tatcgagttg
     1021 tttttccaga ggttcgccaa tccgataatt aacttccagg ccaaagcgag tgtcattctc
     1081 accactcttg ccctgcttat gcccggcact cagggtcaga agaggcactg gcgtgtaatt
     1141 cacttcagcg gtaatcgcat gtggatcttt ctggcgttta tctttaccaa acagcccgac
     1201 ttcatcgcca taatactgtt catacatcag gcttgcgcca agctgcggcc aggcaggtaa
     1261 atagccctca gcacgaatat cccagccatt cgccgggcgt tcctgataat cctcaacatc
     1321 cggcgatttt ttccagccag aagcccggat ataaccattg gcgctcagtt tcaaataatc
     1381 acgccagtat tccgcaccaa caccaatgcg ggtatgacta cgggataaat catgatcgat
     1441 aaaagtattc acccccgcca tccagtcatt ttctgaaaaa tgacgccagc caaaaccaat
     1501 atttgactga gtacgatcgt cggtacgatg tattgccccc tgagtgaaca acatatttgt
     1561 cggtgtatca taaatcggat aaagcatttc cagcgaagag tccttcagcg agaaattttt
     1621 atcgacattc agtttgacgc gcgcagtacc gtatttcccg agccactcct gaatttcctg
     1681 gttagcttta gcggtggcca ttccggtaat aaagttacgt gtcgcatcgc tatctggctg
     1741 actgcttaaa aatgtcccgg cattagcggc aagcgacgcg acatttttct ccacgttatt
     1801 atcagcagtt accgtagtat tttccatgct caaccgtggc tgaaccgcat gctgcgcacg
     1861 tgctgccatt actggcgtaa aggtgacagc gagtggaaaa agaacctgaa cagagatatt
     1921 tgcccacgcc acgcagcggg ccagaactga ataacgaaat cgtggctgtt tattgtctgt
     1981 tttataacgt gacattgaga taatttcctg atgaacgaat taacgaacat aggcagacac
     2041 aaataatggc gtctgcttgt cagaaaaacc ggggatttcc cggtttcctt ttccacagaa
     2101 aaggaaatac ctttatgtaa gaaagtacac cgccacctaa tatatccagt gtctgtagcc
     2161 actcatcagg tggttgggcg cgggattata tttatgggta aaaatttagc tttttgtgca
     2221 agtcattaaa tagatactta agttatattt cttactctgc gggcagtgaa taattcatta
     2281 ttcagagtga ataataatga cccgtcttta attaactgct attgggagta cgtcgaagta
     2341 agtatatacg ctgggataaa ccaatatatt ttttatggac attcattttt attcatcgga
     2401 ctgtaaattc cggggatatc gaacttgcac gactaatctc aaccctcaaa cagatttatg
     2461 aattttgttc ataacccctg acaaatcctg ccgtctaatc gagctcttcc ctttacgtta
     2521 tgcttctcat gaacgataac ggaaggataa caccatgcaa aaagtaaaac tgaacaacgg
     2581 tattgaaatg cccctgttgg gctttggtgt atttcagatg accgatgccg ccgaatgcga
     2641 aagagcggtc attgacgcta ttaacagcgg ttatcgactg attgacaccg ccgcctctta
     2701 tcaaaatgaa atccaggtcg ggaacgcact gaaacaaagc ggtatcgccc gtaacgaact
     2761 ctttgtcacc acgaaactct ggttgcagga taccagttac gaaggggcca aagcccagtt
     2821 cgaacgctct ctgaatcgac tgcaacttga ttacgttgac ctgtacttga ttcaccagcc
     2881 atatggcgat gtccacgggg cctggcgtgc catggaagag ctgcaacagg ccggtaaaat
     2941 tcgcgctatt ggcgtcagta acttccatcc tgaccgactg gcagacctta tcgcttttaa
     3001 taacgtcgtt ccggcggtaa accaggttga agttaaccct ttcaatcagc agctacaggc
     3061 cgttccctgg atgcaaagtc gtggcattca gccagaagcc tgggcaccgt ttgcggaagg
     3121 gaaaaatggt ctgttccagc atcctgttct cacggcaatt ggcgagaagt atggcaaaag
     3181 cgtgggccag gtagttctgc gctggatcta ccaacgaggc attgtttcac tggcaaaatc
     3241 agtgcgaaaa gaacgcatgg aagagaacat caacattctc gattttgaac tcagccctga
     3301 agatatgctg caaattaccg ccctggatac cgcaaccagc gcgttcttct ctcaccgcga
     3361 tcccgcaatg gtggaatggc tgactggccg caaacttgat gtttaagccg cgataaaaga
     3421 ggtattcatt caatgcaaaa acgttatctg ggtaaatccg gactcgaagt ctccgccctt
     3481 gggctcggtt gcatgggctt aagccacggc tacggcccgg cgaccgatac ccgtcaggct
     3541 atcgaactta ttcgtgctgc ggttgaacgt ggcgtcacct tcttcgatac cgccgaagtg
     3601 tatggtccgt atctgaatga agaagtggtc ggcgaagcct taaaaccgtt tcgtgaccgc
     3661 gtggtaattg ccactaaatt cggttttacc ttcggcgatg ataataagca gcagatttta
     3721 aacagccgtc cggaacatat ccgtgaagcc gttgaaggat cattacgtcg tctgaaaaca
     3781 gatgttattg acctgctgta tcaacaccgt gttgatccag atgttcctat tgaagatgtt
     3841 gcaggaacgg tgaaagacct catcgcagaa ggtaaggtca aacatttcgg cctgtctgaa
     3901 gcaggagcac aaaccattcg tcgtgcacat gcggtacaac ctgtcaccgc cctgcaaagc
     3961 gaatattcca tgtggtggcg tgagcctgaa caggaaattc tgccgttgct tgaggaactg
     4021 ggaattggtt ttgtcccctt cagcccacta ggtaaaggtt tcctgacagg ggcgattaag
     4081 ccgggaacca ctttcggcaa agatgattac cgcagcaccg taccgcgttt cgcagcacaa
     4141 gccattgaag ccaatgaaaa actggtcacc ttgttaggtg aactggctgc ggagaaaggg
     4201 gtaacgtctg cacaaattgc actggcatgg ctattagcac aaaagccgtg gattgttccc
     4261 atccctggca ccactaaact gcaccggctg gaagaaaacc tggccgctgc tgacattgtt
     4321 ctttcgcaga aagatacgca gcagataagc gaggcgctgg aaacaattaa aatcgtcggt
     4381 gaacgttact cgcctgaaca tcaggcacgc gtaggccgct aatacccaaa cgggtccagg
     4441 ataaaggacc cgttattcgg tgtagcgaag cgcatcgatc aacaacgcaa aagctggcgg
     4501 atgctgcttg cggctggggt agtaaagata atacccagga aaagttggac accactcctg
     4561 taacacctga ataagcttgc ctgactctat atactcctga accatgtctt cagggacaca
     4621 agcgatccca aaccctgata acgcggcatc aattctttct ggcaatggac tacacgtcag
     4681 ttgcccatca accttaacgc gaagcggttt cccttctcgc tcgaactccc agtggtaaag
     4741 cccaccggca gttggcaaac gcatgttgat gcagcgatga ttttgtagct cgtgaggcgt
     4801 ttcaggcgca gggtttgcag caaaatacgc cggagctccc accacagcca tccgcatatc
     4861 tggtccaatc ctgactgcaa ccatatcttt atcaacattt tcacccagtc gtatcccggc
     4921 atcaaaacgg ccttcaacaa tatcgacaaa accgttatcc accaccagtt cgacattgat
     4981 ttcgggatac tctctgagaa aaggttttag cttcggccag accagacttc gcgcggcatg
     5041 ttccccggca gataaccgaa tattcccgga gggcgtgccg ttcaattgaa taagtgattc
     5101 cagttcctgt tcaagctcgt caatacgcgg ttcaaggcag gcaatgattc tctcccccgc
     5161 ttctgtggga gccacactgc gggtagtccg tgtcaggagg cggatattga gcctttcctc
     5221 cagagccttc attgcatggc taagtgcaga ctgagagacg ccgagtttgc ccg
//
LOCUS       DAWDRC010000250         5272 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6944_length_5272_cov_2.701936, whole genome shotgun sequence.
ACCESSION   DAWDRC010000250 DAWDRC010000000
VERSION     DAWDRC010000250.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6944_length_5272_cov_2.701936"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1392 a         1328 c         1256 g         1296 t
ORIGIN      
        1 gcgtttttcg ctattccgtc actctcgccc agcaaataag aggcgaagac agcataacct
       61 gtggtgcaac gcgccagaaa cgcttcgtcg tgccaaccat tttccaccag cgtatgggcg
      121 atccccagca tcaacgcaac atcggtgccc atgtgtggtg ccacccactc cattttatcg
      181 ccaaagaaat cgatggtttc cgatcgcatt ggatcaatgc agatcagctt tttcccgctg
      241 tcacgcagtg cggaaaagta agaaagcccc tgctcatcgg atgcattcca cgcaattttc
      301 agcgtattga gtgggttagc actccacagc accacgacat cgctatgttc cagcaccagc
      361 ggccaactgg tttgctgttg ataaacttcg ctgccaccca cgacatacgg catgatcgcc
      421 tgtgccgcgc cggtcgaata atcccccaga tgcccagtat aaccgcctgc cagcgccata
      481 tagcgttgta ataatgtcga ggccttatgc agcacgccgt ttgaacgcca gccgtaggaa
      541 ccagcaaaaa tcgatgccgg accataagcc tcacgaatgc gtttatgttg atggtgaata
      601 agctccagcg cctcatccca actcacgcga acaaattcat cctgcccacg aatgccttgc
      661 ggattttccg gtgacgcaag aaagcctttg cgcaccattg gaaatcgcac ccgcgtattg
      721 ctgtgaacct ggtcgcgaac cgcgctctgc aaggagtttt ccattcctgt ggctaacgcg
      781 ccacgcgagc taaacacggt ttcgccgtcg gtttcaacca gcataggccc ccagtgggcg
      841 gcagtcagaa cggaatatcg tgaggatgag ttggtcaaac cgaagactcc tgaaaggtta
      901 gatgtattga gcagttaaat attacttaca aatttccgag tattcccagg aataatcttc
      961 atgttcacgc ggcataatct cccgccacgg aacccgtggc aaagaataaa aaggttatta
     1021 aggattaaca atgaagaaac gtgtttatct tattgccgcc gtagtgagtg gcgctctggc
     1081 ggtatctggc tgtacaacta acccttacac cggcgaacgc gaagcaggta aatctgctat
     1141 cggcgcaggt ctgggctctc tcgttggcgc gggtattggt gcgctctctt cttcgaagaa
     1201 agatcgcggt aaaggcgcgc tcattggcgc agcagcaggc gcagctctgg gcggcggcgt
     1261 tggttattac atggatgtgc aggaagcgaa gctgcgtgac aaaatgcgcg gcactggtgt
     1321 tagcgtaacc cgcagcgggg ataacattat cctcaatatg ccgaacaatg tgaccttcga
     1381 cagcagcagc gcaaccctga aaccggcggg cgctaacacc ctgaccggcg tggcaatggt
     1441 actgaaagag tatccgaaaa cggcggttaa cgtgattggt tataccgaca gcacggggag
     1501 tcacgacctg aacatgcgtc tctcccagca gcgtgcggat tccgttgcca gcgcgttgat
     1561 cacccagggc gtggacgcca gccgcatccg tactcaaggc cttggcccgg ctaacccaat
     1621 cgccagcaac agcacctcag aaggtaaggc gcaaaaccgc cgtgtagaaa ttaccttaag
     1681 cccactgtaa tccctttcat gtcaggtgat gcagaaatca cctggcattc ttcagttttc
     1741 cttcatcata tttcaggcta aggtgatcgc cttatcagtg aatggagaga agcatgaagc
     1801 cgtccgttat cctctacaaa gccttacctg atgatttact gcaacgcctg caagaacatt
     1861 tcaccgttca ccaggtggca aacctcagcc cacaaaccgt cgaacaaaat gcagcaattt
     1921 ttgccgaagc tgaaggcttg ctgggttcaa acgagaatgt tgatgccgca ttgctggaaa
     1981 aaatgccgaa actgcgtgcc acatcaacga tctccgtcgg ctatgacaat tttgatgtcg
     2041 atgcgcttac cgcccgaaaa attctgctga tgcacacacc aaccgtatta acagaaaccg
     2101 tcgccgatac gctgatggcg ctggtgttgt ctaccgctcg tcgggttgta gaggtagcag
     2161 aacgggtaaa agcaggcgaa tggaccgcga gcataggccc ggactggtac ggcactgacg
     2221 ttcaccataa aacactgggc attgtcggga tgggacggat cggcatggcg ctggcacaac
     2281 gtgcgcactt tggcttcaac atgcccatcc tctataacgc gcgtcgccac cataaagaag
     2341 cagaagaacg cttcaacgcc cgctactgcg atttggatac tctgttacaa gagtcagatt
     2401 tcgtttgcct gatcctgccg ttaacggatg agacgcatca tctgtttggc gcagaacaat
     2461 tcgccaaaat gaaatcctcc gccattttca ttaatgccgg acgtggcccg gtggttgatg
     2521 aaaatgcact gattgctgca ttgcagaaag gggaaattca cgccgccggg ctggatgtct
     2581 tcgaacaaga gccactgtcc gtagattcgc cgttgctctc aatggccaac gtcgtcgcag
     2641 taccgcatat tggatctgcc actcatgaga cgcgttatgg catggccgcc tgtgccgtgg
     2701 ataatttgat tgatgcgtta caaggaaagg ttgagaagaa ctgtgtgaat ccgcacgtcg
     2761 cggactaagc cgcgactgcg tggagtaaag cccgataatc gctcgggctt ttactcttta
     2821 ttgggttgca gtaactgctg tagtccaggc ctgattaaac gcctgatgtt gtgccggtaa
     2881 tggcgcaatc agtttgttat attcacttgc ctgctgtgaa gtcgggaact ggatgccatt
     2941 tgcgacaaag ctcacctgag taccttgctg agcaatgtag tctccaacca tcaccagttg
     3001 ttgcgtgaaa gtttgtgctg ccggaatcaa cggttgcaat gcatcagctg gcgtcgttac
     3061 cactttggtg aatgcctgat caaagaccgg tttcaggtca tcactctgtt ttaacgcaga
     3121 gtgcgcagca tcggcctgca acttcgcatt ttgcagctgt tgcgccagca cacccagcga
     3181 accgttcatt tcacgcagcg gaccgctttg cgtaacgtaa tcctgcggta cgcgaattgc
     3241 gttaacgctg tctacaaccg ggcgcagacc ggaatccatc gcctgattca cctgctgaga
     3301 ataaccatac agaatcgcgt aatcagagac aaaaggacca aactgttttt tctgatcggc
     3361 agtcagggtt ggtagacgtt caccgctacg catcactgta ttctgcagga agtcgataaa
     3421 cgctttacgc tgatcgcctt ctttatcaaa acacccactc aggctaacta ccattaataa
     3481 cgccgcaaga ggcgcaaacc agcgagagca ggactttcct gtcgccattt tcttactcct
     3541 ttcacccaaa aaaagcgcac aacgacacac gcgtgtctga cgtgacaagg atagtccagg
     3601 acaggcttgc aggataccct ttctacgtaa tcttcctagt aaaatcatgg agaaaatgaa
     3661 acaaggttaa tttgttgttt cattgttaaa aaatgatatt gctgttgctt tttttgtagc
     3721 cagagattta cgccttcgca gaacgttaga ttttcgaaat ctttacaaac cgggagtggt
     3781 aagagatata tcggcattta tcagatcgtt aggtggctat aagtcacgct aaatgactgg
     3841 ctgaatcgaa tcatagtcag agtatgccct gacaacaccc cgctgtgtct gtttttcccg
     3901 actattctta atgagcttcg atgcaattca cgatcccgca gtgtgatttg aggagttttc
     3961 aatggaatat aaagatccaa tgcatgagct gttgagcagc ctggaacaga ttgtttttaa
     4021 agatgaaacg cagaaaatta ccctgacgca cagaacaacg tcctgtaccg aaattgagca
     4081 gttacgaaaa gggacaggat taaaaatcga tgatttcgcc cgggttttgg gcgtatcagt
     4141 cgccatggta aaggaatggg aatccagacg cgtgaagcct tcaagtgccg aactaaaact
     4201 gatgcgtttg attcaagcca acccggcatt aagtaagcag ttgatggaat agacttttat
     4261 ccactttatt actgtttacg gtcctgataa caggaccgtt ttccaaccga ttaatcataa
     4321 atatgaaaaa taattgttgc atcacccacc aatgcgtggc ttaatgcaca tcaacggttt
     4381 gacgtacaga ccattaaagc agtgtagtaa ggcaagtccc ttcaagagtt atcgttgata
     4441 cccctcgtag tgcacattcc tttaacgctt caaaatctgt aaagcacgcc atatcgccga
     4501 aaggcacaat taattattaa aggtaataca ctatgtccgg taaaatgact ggtatcgtaa
     4561 aatggttcaa cgctgacaaa ggcttcggct tcatcactcc tgacgatggc tctaaagacg
     4621 tgttcgtaca cttctctgct atccagaacg atggttacaa atctctggac gaaggtcaga
     4681 aagtgtcctt caccatcgaa agcggcgcta aaggcccggc agctggtaac gtaaccagcc
     4741 tgtaatctct gcttaaaagc acagaatcta ggatccctgc catttggcgg ggattttttt
     4801 atttgttttt caggaaataa ataatcgatc gcgtaataga atctagtgtt atttttgtga
     4861 agaataaatt tgggtgcaat gagaatgcgc aacaccgtaa gaaaggcggg aataatttcc
     4921 cgccgaagac gcttactctt tcaatttgca ggctaaaaac gccgccagct cataactctc
     4981 ctgtttaata tgcaattcac acagtgaatc tcttatcatc caggtgaaaa ataaaagcgt
     5041 gaaacaaatc actattaaag aaagtaatct atatttctgc ggcattccca gccccctgtg
     5101 ttgatttcca acgagtaaga ggctaacctt atggtgtcta tgcatagagg ggcctcactt
     5161 tgatttatag tcaggtgggg cttttctctg tctgcctttc ggtgaatacc tgagacaaac
     5221 agtctcaagc acccgtggct attccagctt aataagttcg tttcttctcc tt
//
LOCUS       DAWDRC010000251         5271 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6947_length_5271_cov_2.072853, whole genome shotgun sequence.
ACCESSION   DAWDRC010000251 DAWDRC010000000
VERSION     DAWDRC010000251.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6947_length_5271_cov_2.072853"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2903..2912
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4104..4113
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4627..4636
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1335 a         1323 c         1321 g         1262 t
ORIGIN      
        1 tcacctgcaa tagcagtgaa accaggacca caaagaaggc gacattaaag aacagtcgcg
       61 cattctccag ccccgccatc atcgggaaca cagccagaat aatcggcacc gcgccgcgta
      121 atcctaccca gctgataaac acgcgctcac gcagattgaa gccacggaac ggtagcaatc
      181 cggcaaatac cgaaagagga cgggcgaaga aaatcatcca tgcggacaaa atgagcgccg
      241 gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc aggaacatgg
      301 cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag cggttgcgaa
      361 tcggccgatt acccagcaga aaaccgaaca gataaacagc cagaataccg ctgccttcca
      421 gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat ggatataatc
      481 cggcgggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg ccaagcccga
      541 tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa ctgacactgc
      601 tttcatgttg ctggatcatc gcaatcaggg taatcgtcag aaagaccgcc attggatcat
      661 tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc ttaccaccca
      721 gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt aataatcctt
      781 caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca gaggtgataa
      841 gcacgcccag cgtcgccagc gacagtgccg gtcctaacgc cacacgaaag gaactggcct
      901 gagtacgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt accatgtagg
      961 cgaaggggta attatcaaac gggataccgc cgacgccatc aacccccgcc agcatgccga
     1021 tcgccagaaa aataaccaga atgggaatgc caagacggga agaaaatgaa ctaagtaata
     1081 tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt gtggcatcca
     1141 acggtcggtt actcctgatt acgctgtctc tgatataaac cctaccatat tagcggcaga
     1201 gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac aggatgaccg
     1261 ctaatcgtca actgggtgcc ttcctgggtg ttattaagaa tggcatgtgc gcccaacggc
     1321 agtgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg aatcgacagg
     1381 cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata atcattaggc
     1441 gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat tccagcatga
     1501 tagagctgca acagcatacg ttcgacgcgg aaagggtgct cgttaatatc ttccagcacc
     1561 agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat cagcatcgca
     1621 agattgcctc cccacagcgt gccttcggtc tgacatgtcg ggccttcccc ttgccattca
     1681 atggtgaacg ttttattgcg taacgccagc cagaagtggt gctcggtaaa ggcatttagt
     1741 tcatccgcgc caaagtttgc taccagcatg gggccgctaa aggtgatgac attgccttga
     1801 gccagaagac cgaactgaat ggcggtaaaa tcgctatgtc cgcaaatgag taacgggtca
     1861 tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg actggcaccg
     1921 taaccgccgc gtacagacag cacgatggtg ttgggggtag tcagtctggc aagagaattg
     1981 agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg ggcaataacc
     2041 tcgacgttat tgacctgatg ccccgcgtcg gtaaggcgct ggataccacg cagcgcggcg
     2101 tgctgtttaa tgcagtaacc cgatggggca attaagtgaa acagagacat ggcaattcct
     2161 tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg tcacctcaac
     2221 ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc tttttgattg
     2281 tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg aacgcgaaag
     2341 ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt gcagcctggg
     2401 gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt aatccgaacg
     2461 cggtgagtaa atcgaatgcc attggtttga tgcagataaa agcttcaacc tccggacgtg
     2521 atgtatatcg tcgtatgggc tggagcggtg agccgacgac cagcgagctg aaaaatccgg
     2581 agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc ccgctggcag
     2641 gcattgaaga tccgaaggta ctgcaatatg cgctggtggt gtcatacgct aacggggcag
     2701 gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa atcaacgatc
     2761 tggatgctga tgagttcctc gatcacgtag cgcgaaatca tcctgcgccg caggctccgc
     2821 gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcac gcacattgtc
     2881 cgcttttttc cgggcttctc gcnnnnnnnn nncccatgcc gcctaacgac aaatcataca
     2941 ggcggaaacg taacgtactg ttatccgaca gtttggtctg gcaaaaataa ggcggatgga
     3001 gtggggcggt gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta
     3061 cggtaataaa tgccggaagc agcaagtatt cactctgctg tagttgttca acagtaaact
     3121 cgactttcgc accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga
     3181 tgttgtcttc ggcctggctg ccgaaatcca gcaccagttt atccggagtt attgccagta
     3241 ttttgctgat cagctgcccg ccattccagc tgatacgcaa aggaattgcg gctttgttca
     3301 aatcgcgtaa cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat
     3361 gactcacgcg gaaaaactcc tgactgacaa actgtctttg gtttatcggt cgcagttaac
     3421 aaaacttaat acaaatgcgc gaatattttt tacatgttgt tcttaaatca gccgacatac
     3481 gcccagcatc gatcccaccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcgt
     3541 ccgattcatc gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg
     3601 atagccggta ttatcgccaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg
     3661 acctgtattc ttgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc
     3721 attggcggct ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct
     3781 atcctggtgc tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat
     3841 agcgatacag atgccgctat ccgctttcac atcagaacgt atactcgata ccagcccaga
     3901 aattgcgacc ttcttcaata aacccttcac tgtaggcata tttggtatca aacaggttat
     3961 tgaccgacgc attaacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca
     4021 ccgcaaaacc ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct
     4081 cttccgacag cgttacgctt aacnnnnnnn nnncttaacg ggtccaagga gttgtgagtc
     4141 atccatgcgg tcattgtctg cgttggcaga tcggttatct tgccgatgtc tttacgtttg
     4201 gcatcagcgt ggatcagggc gtagctcaat cctacatcca gtatattgct gattttcccc
     4261 ttaataccgg catccagacc gctgtaatcc accgtgccgc tgttctgatt ttgaatggta
     4321 tcggcatcga tattgtgcga gaggatggca tcactcaccc ggttatagta aacgctgacc
     4381 tcaaatcccc agtcgtgcgt gaaggcacca ttccaggtta aatccacccc gcgcgcgcgt
     4441 tccggcttga gctgcgggtt aacaatcgct atctggttat acgcaggttt ggacgtggta
     4501 tagcgttctt tcagcgtcgg aaagcgtgtg cggtcatagt acgaaagcgc cagcgtgtct
     4561 tcattggcaa agtggtattt ccccatcacc tgccagttaa aagctgactg attgttgtcg
     4621 tcataannnn nnnnnnatac tgccatcttt ctcatgtttt ttcgcttcta cgctatcgcg
     4681 ccagtcatag ctgattccag ccacgacatc gacattatcg gcagcagccc attgatattc
     4741 actggcgagc gaccaggtac gatcttcata gcgatcgtaa gcggcgtgcg gcgcaccttt
     4801 ttcccggtgt acgtcatctt tccagttaac ggcaaacgac agcagatcgt tttcacgcac
     4861 atcggctgcc agttgtaacc cggcaccgtc gctgtaatcg gaataatggc tgtagctgcc
     4921 ttttttattt ttcaaatcag ccagcgagtt gtacatcatt agcgtatttt caaaggtgtc
     4981 gcgatacagc cgacttttca gggtaaaacg atcgtttagt tgggtcgttc cctgataata
     5041 aaaactttct ttgtcatact ctggccactg ccagtagcgt gatttctgac cactatttcc
     5101 actgtatggc gggttatctt tttcaccatc ctgcttaatg taagtcaatg tgtattcatc
     5161 gttttcgcgt ggtgtaaaac cgagcttcac aatgccgcgt ttatcatcag ccgatgaatt
     5221 aatcatcttg ccgtgtttgc ctgcaatatc attatttaca ccatgctgca g
//
LOCUS       DAWDRC010000252         5255 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6961_length_5255_cov_2.112692, whole genome shotgun sequence.
ACCESSION   DAWDRC010000252 DAWDRC010000000
VERSION     DAWDRC010000252.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6961_length_5255_cov_2.112692"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2419..2428
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1227 a         1416 c         1428 g         1174 t
ORIGIN      
        1 ggatagcgac gcagcggttg caaattcact tcgcacgcca gctcggcatt tttgcacagc
       61 gacataaaat cgcccgcctg ggcgcgcgtg gctttatatt ccggtagata gcgacccgcc
      121 tggcgcatca tccatactgg agtgacatca acgggctggc gcagcagcgc ccgcagataa
      181 cgatcgtttt taagttcggt cattttggct gttccttagt gcgtcaggcg gtcagtgtat
      241 catcactcat actctgcccg acacatcgcc actgtatctt ctatcagacg gcgcgctacg
      301 gtgccgggcg gcgggagtaa cggcaaatcg tcatagcgat accagttcgc ctcaagcaat
      361 tctttcgggt cgatcacgat ctcgccgctg tcatattccg ccataaacgc ggtcattaac
      421 gactgaggaa acggccacgg ttgggaggtc acgtaacgca agtttttaac tttaattccg
      481 ctctcttcca tcacttcccg cgcgactgcc tgctcgaggg tttcacccac ttcgacgaat
      541 ccggcaagta ctgtatggac accgttacga tggcgggtat gctgggcgag gaggatcgaa
      601 tcatcgcgac ggatggcaac aataatgcag ggggcgattt gcgggtagta acgctcacgg
      661 caatggctgc acagcatcgc ccattcggtt ttgcttggat acatttcatg cccgcagtaa
      721 ccacagtatt tatgcgatcg gtaaaactcc gccagttgca cgccacgtcc ggccagttga
      781 aacagcccaa catcgagatc aatgacctga cgtaccgacc ccatatcgtg acgccgctgc
      841 agttgtacta accaaacagg ttccccctgc cattcgccga tttgtagtgc gcgctgaccc
      901 acaagatcga aatttgccgc ttcgccatat ggcaattctc ccttcggcaa ccataatttt
      961 tgttcatggc tgacgaccca ccagccgtga tctaattttt caattatacg atccatagct
     1021 cttgcactac ctttgcatca ctggcatgtt taacatggtt tttacatttc tcacagagca
     1081 gtttttgaat acaaacttgc ggagtcaatc atgcttaacc agctcgataa cctgacggag
     1141 cgcgtcagag gaagtaacaa attggtggat cgttggtgac atgtacgtaa gcatctgctc
     1201 gtggcttact acaatctggt tggcattaag cctggcaaag aatcgtacat gaggctaaac
     1261 gaaaaagccc ttgatgattt ttgtcagagc ctggtcgatt acttgtctgc cggacatttc
     1321 agtatttatg agcgcattct tcataagctg gaaggcaatg ggcaactcgc acgcgccgcg
     1381 aagatttggc cacaactcga agccaatact caacagatta tggatgacta cgattccagt
     1441 ctggaaaccg ccatcgatca tgataattac cttgagtttc aacaggtttt atctgacatc
     1501 ggcgaggcac tggaagcgcg ctttgtgctg gaagataagc tgattctact ggtgcttgac
     1561 gccgcccgcg tcaaatatcc tgcttgagtt ctgcgctgtt aacgcgtaat ttacattcaa
     1621 cgccccgttt gtggggctaa tttcttgtcg gagtgcctta actggctgag accgtttatt
     1681 cgggatccgc ggaacctgat caggctaata cctgcgaagg gaacaagagt taatctgcta
     1741 tcgcatcgcc tttgcggcga tcgtctcttg cttcatccgt cgtctgacaa gccacgtcct
     1801 taactttttg gaacgagcta tgtctgcaac aaaactgacc cgccgcgaac aacgcgccca
     1861 ggcccaacat tttatcgaca ccctggaagg ctccgccttt cccaactcaa aacgcattta
     1921 catcactggc acacaccccg gcgtacgcgt gccgatgcgt gagattcagc ttagcccgac
     1981 gctaattggc ggcagcaaag aacagccgca gtacgaagaa aacgaagcga ttccggtcta
     2041 cgacacctcc ggcccgtatg gcgatccgca gattgccatt aacgtgcagc aagggctggc
     2101 aaaactacgc cagccgtgga tcgatgcgcg cggcgatacc gaagaactta ccgtgcgcag
     2161 ttccgattac acaaaagcgc ggctggcaga tgatggcctc gacgagctgc gttttagcgg
     2221 cgtattaacc ccaaaacgcg ccaaagcagg ccaccgcgtc acacaactgc actacgcccg
     2281 caagggtatc atcacgccag aaatggaatt catcgccatt cgcgagaata tgggtcgcga
     2341 gcgcatacgt agcgaagttt tacgccacca gcatccggga atgagctttg gcgcacgtct
     2401 gccggaaaat atcactgcnn nnnnnnnncg gccaacatta atcatccgga atcggagccg
     2461 atgattattg gccgtaactt cctggtaaaa gttaacgcca atatcggcaa ctcagcggtc
     2521 acttcttcca tcgaagaaga agtggaaaaa ctggtatggt ccacacgctg gggagcggat
     2581 accgtgatgg atctttccac tggtcgctat attcacgaaa cccgcgagtg gattttgcgt
     2641 aacagcccgg tgccgatcgg tacagtgccg atctaccagg cactggagaa ggttaacggg
     2701 atcgccgaag atcttagctg ggaagcgttc cgcgacacgc tgctggaaca agccgagcaa
     2761 ggtgtggatt acttcactat ccatgcgggc gtactgctgc gctatgtgcc gatgaccgcg
     2821 aaacgcctga ctggtatcgt ctctcgcggc ggttcaatta tggcgaaatg gtgcctctcc
     2881 catcatcagg aaaatttcct ctatcaacac ttccgcgaaa tttgtgaaat ctgtgccgct
     2941 tatgacgttt cgctgtcgct gggcgacggt ctgcgccccg gttctgttca ggacgccaac
     3001 gatgaagcgc aatttgccga gctgcatacg ctgggtgaac tgaccaaaat cgcctgggaa
     3061 tatgacgtac aggtgatgat tgaaggccca ggccacgtgc cgatgcagat gattcgccgc
     3121 aatatgaccg aggagttaga gcactgccac gaagcgccgt tttacactct ggggccgcta
     3181 actaccgata tcgcgccggg ctatgaccac ttcacgtcag ggattggcgc ggcgatgatt
     3241 ggctggtttg gctgcgcgat gctctgctac gtaacgccga aagagcatct gggcctgcca
     3301 aataaagaag atgttaaaca ggggcttatc acctataaga ttgccgctca cgccgccgac
     3361 ctggcgaaag ggcatccggg cgcgcaaatt cgcgataacg ccatgtcgaa agcccgcttc
     3421 gaatttcgct gggaagacca gtttaatctg gccctcgacc cgtttactgc ccgcgcttat
     3481 cacgatgaaa ccctgccgca agagtccggt aaagtcgccc atttttgctc catgtgtgga
     3541 ccgaaattct gctcgatgaa aatcagccag gaagtgcgtg attacgccgc cgcgcaaacc
     3601 attgaagtgg gaatggcgga tatgtcggag aacttccgcg ccagaggcgg agaaatctac
     3661 ctgcgtaagg aggaagcgtg atgtatcagc ctgagtttcc tcctgtaccc tttcgtttag
     3721 gactgtaccc ggtggttgac agcgtacagt ggatcgaacg tctgttggat gcaggcgtac
     3781 gtactctcca gctacgcatc aaagatcggc gcgatgaaga ggtagaagcc gatgtggtgg
     3841 cggcaattgc gctgggccgc cgctataacg cgcgattgtt tatcaacgat tactggcggc
     3901 tggcgattaa gcatcaggcg tatggcgtcc atctggggca ggaagatttg caagccaccg
     3961 atctcagcgc catccgcgcg gcaggcctgc ggctgggcgt ttcgacccat gacgatatgg
     4021 agatcgacgt cgcgctggcg gaacgtcctt cttatatcgc gctgggacat gtgtttccga
     4081 cacaaaccaa acagatgcct tctgcaccgc agggactgga acagctggca cggcatgttg
     4141 agcgcctggc ggattatccc accgtggcga ttggcggtat cagtctggcg cgcgcgcctg
     4201 cggtgatagc aacgggtgtc ggcagtatcg ccgtcgtcag cgccattact caagccgcag
     4261 actggcgttt ggcaacggca cagctgctgg aaattgcagg agttggcgat gaatgaccgt
     4321 gactttatgc gttatagccg ccaaatcctg ctcgacgata tcgctctgga cgggcagcaa
     4381 aaactgctcg acagccaggt gctgattatt ggtcttggcg ggctgggtac acctgctgcg
     4441 ctatacctgg cgggggctgg cgtcgggacg ctggtactgg cagatgacga cgatgtgcat
     4501 ttaagcaatc tgcaacgaca aatcctcttt accactgaag atatcgatcg cccgaaatca
     4561 caggtcagcc aacagcgact gacacagttg aatcccgaca ttcaactgat ggcattacaa
     4621 caacgattaa cgggtgagac gttaaaagat gcggttgcac aagccgatgt ggtgctcgac
     4681 tgtactgaca atatggcgac tcgccaggag attaatgcca cctgcgtggc actcaacacg
     4741 ccgcttatca ccgccagcgc ggtcggattt ggcggtcagt tgatggtact gacgccgccc
     4801 tgggagcagg ggtgttaccg ctgcctgtgg ccagataacc aggagccaga acgcaactgc
     4861 cgcacggcgg gcgtggttgg cccggtggtc ggggttatgg gcactttgca ggccctggaa
     4921 gccattaagt tattaagcgg tatagagacg cctgcgggag aactccgact gttcgacggt
     4981 aaatcgagcc agtggcgcag cctgacgttg cgccgcgcca acggttgccc ggtatgtgga
     5041 ggatgcaatg cagatcctgt ttaacgatca accgatgcag tgtgtcgccg gactaactgt
     5101 tcacgaactg ctgaagcaac tcgatcaaca acaagcaggt gcggcactgg cgattaatca
     5161 gcaaatcgtc ccgcgggaac aatgggcgca acatatcgtg caggatggcg accagatcct
     5221 gctttttcaa gttattgcag ggggttgaga tgtta
//
LOCUS       DAWDRC010000253         5226 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6994_length_5226_cov_2.748018, whole genome shotgun sequence.
ACCESSION   DAWDRC010000253 DAWDRC010000000
VERSION     DAWDRC010000253.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6994_length_5226_cov_2.748018"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3800..3809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1265 a         1392 c         1368 g         1191 t
ORIGIN      
        1 tgagtatcaa aaagccggtt aactgaaact gtccaggttt tggggttcag ttcatcacgc
       61 cgcatccggc ataaacaacg cgcactttgc caacaatctg aagggccgca tttgcggccc
      121 ttctcacaaa gcatcttacc gattacagca gcgcttttgc tttcgcaaca acgttgtcaa
      181 cggtgaagcc gaactcttca aacagctgct ctgccggagc agattcaccg aaggtggtca
      241 tgccgacgat agcaccgttc aggccaacat acttgtacca gtagtcagca atgccagctt
      301 ccaccgcaac acgagcggta accgctttcg gcagtacgga ttcacggtaa gccgcgtcct
      361 gcttgtcgaa tgcgtcggta gacggcatgg aaaccacgcg cgctttcacg ccttcggcag
      421 tcagtttttc gtaggcggct acagccagtt caacttcaga accggtcgcg atgaagatca
      481 gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg tttgccagct
      541 gctcttcagt acgttcctgc tgcgccaggt tctgacggga gaggataagc gcagtcgggc
      601 cgtcctgacg ctcaacgccg tatttccacg ctactgcgga ttcaacctgg tcacacggac
      661 gccatgtaga catgttcggg gtcacgcgca gagaagcgat ctgttctacc ggctggtgag
      721 tcgggccatc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc tgacgctgtt
      781 tcatcagcgc agccatacgt acggcattac gtgcgtattc cacgaacatc aggaaggttg
      841 aggtatacgg caggaaacca ccgtgcaggg agataccgtt ggcaatcgca gtcataccga
      901 attcacgtac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt gctttagaac
      961 cagaccacag ggtcaggtta gacggcgcca ggtcagcgga gccgccgagg aattccggca
     1021 acagcggccc gaacgcttcg atagcattct gagacgcttt acggctggcg atcttcgccg
     1081 gattagcctg aagtttagcg atgaactctt tcgctttggc gtcgaagtca gacggcattt
     1141 cacctttcat acggcgggta aattcagcgg cttcctgcgg ataagctttc gcgtaagcag
     1201 cgaatttctc gttccatgcg gactctttcg cctggcctgc ttctttcgca tcccactgag
     1261 catagatttc agacgggatt tcgaacggcg cgtatttcca gcccagctgt tcgcgggtca
     1321 gggcaatttc agcgtcgccc agcggcgcac cgtgggagtc gtgggtaccg gctttgttcg
     1381 gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca gtcactgcgc
     1441 gcgcttcttc tactgcgcgt ttgatggatg ccgcgtcatg accgtcgatg tcgcgaataa
     1501 cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtaaaccag ccttcaacgt
     1561 gaccatcgat agaaataccg ttgtcatcgt agaacgcaat cagtttaccc agcttcagcg
     1621 tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg tcgcccatga
     1681 aggcgtaggt gtagtggtcg acgatgtcgt ggcccggacg gttaaactgc gccgccagcg
     1741 ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc ggaccggtgg
     1801 tggtttctac acctgcggtg taacccactt ccgggtgacc cggagtttta gagtgcagct
     1861 gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc agcaggctgt
     1921 agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca gcccaggacg
     1981 gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg tcagccatac
     2041 ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg ctcagcgcac
     2101 gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg atgatgaagg
     2161 gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa tttgccaaca
     2221 atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc agaaatctct
     2281 ggatcatgct cgcatgttgc gcaatccact cgcccgtccg ctgcgctttt ccttatactg
     2341 agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg accttgtgcg
     2401 gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggct acggtgctga
     2461 ccggttgcca gaatatggac tccagtggac tgctctcatc aggagcggaa gcttttcagg
     2521 cttacagttt gagtgatgcg caggtgaaaa aactgagcga tcaggcatgc caggagatgg
     2581 acagcaaagc gacgattgcg ccagccaata gcgaatacgc taaacgtctg acgacaattt
     2641 ctcgcgcgtt aggcgataac atcaacggtc agccggtgaa ttacaaagtg tatatggcga
     2701 aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc gggctgatgg
     2761 atatgatgac ggataacgaa gtcgaagcgg tgctcggtca cgaaatgggg cacgtggcat
     2821 taggccatgt aaagaaagga atgcaggtag cacttggtac aaacgcaatt cgcgttgcgg
     2881 cggcctctgc aggcggaatt gtcggcagtt tgtcacaatc acaactggga gacctgggcg
     2941 aaaagctagt caattcgcaa tttacccaac gtcaggaatc agaggccgat gattactcct
     3001 acgatttgct gcgccaacgc ggtatcagcc cggcaggtct tgccaccagc tttgaaaaac
     3061 tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatcct gcatccgccg
     3121 aacgcgccca gcatattcgc gatcgtatga gcgcggatgg agttaagtaa agccaggtag
     3181 tgccgaacgt aggtcggata agacgttcac gccgcatccg acattaatga cacatcactc
     3241 gtgcgcctct tatctggcgc ttactcgccc tttttcgccg cctggatata cagcatctcc
     3301 agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccacc
     3361 tccactacgt ccatcccaac gatgttgaga tctttcaggc cgcgtactag tttaatggcg
     3421 cgatcggagg tcaggccgcc aattaccggc gtaccggtgc ctggtgcaaa agcaggatcc
     3481 aggcagtcga tatcaaaggt caggtaaacc ggcatatcac ccacaatctg tttcacctgg
     3541 gcgataatgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
     3601 ttgtctttat caaactcggt acgaatacca atctgcacag aatgattcgg gtcgatcaga
     3661 ccttctttcg gcgcggtata gaacatggtg ccgtggtcaa attcacaacc gttagcgtat
     3721 gtatcggtgt gggcatcaaa gtgtaccaac gccattttgc cgaaatgctt cgcatgagcg
     3781 cgcagcagcg gcagcggaan nnnnnnnnna gcgtaacaaa gtggtcacca ccgaaggaga
     3841 gcatacgctt accggcagcc agcagcttct cggcatgcgc ctgcagcttc tcgctcatct
     3901 cacgggcatc tccgaaggca tataccagat cgccgcagtc cacgacgttc aggcgctcac
     3961 gcatgtcgaa attccacggg aagcgattgt gttcccaggc cagattcgtc gaaacctgac
     4021 ggatcgctgc cggaccgtgg cgaccacccg cacgaccaga agtggccata tcgaacggca
     4081 cgccagtaat cacccagtca gcatcgctgt catatggctg gaagttcatc ggcaggcgta
     4141 aaaaaccaaa ggcgttggaa accagtgagt tatcgtattg atgacctaag gtgctcatgg
     4201 atggactcct attacaaagt cgatataaaa aaacccttcc gcgtcgttag gcccgacgag
     4261 gaagggttgg atttgtgaaa cacaagtttg tgctaattat cgccttaaat gggcgaggca
     4321 tcaagcgagg ttgctatttc cgacacggtg gcggcgcata gccattgtta tctgccggaa
     4381 aggcttgtcc agagcacggc ggcagcgcgt ttttcgccgg gccttgtaat tcaaaggcgg
     4441 caaagccgat tacaccggta ttacgcacat cgccttgcgc cgaattggct gcgtaggaat
     4501 cagcaacgtt ggcgaactga aaagccgctt cggtatgttt atctttgcgg aatcctttaa
     4561 tcgctaacga atctccggca ttcacaatat aaccgttgtt attcagcgaa ccttgcttgc
     4621 cgttcagcac atccaggcca tcaaccgtag caacgatctc gtagttggtg ttacgactgt
     4681 aattacggac atataattga taattcattc cgactctggc atggacaata tagcgtggtt
     4741 cattattgga tgaatcataa acccgcgtaa taggtagaga attaaaatta ccatcgcgaa
     4801 tagcatattc cagttcgtta atacgaatgc tataaacgcg gtcgtagccg gaaggaacat
     4861 tagtcgaata actaataacg atcatctctg ccggatcgcg ccaggctcgt tcagcagata
     4921 cgccatgaac cgttgagtgc acatccccac cccaggcggt gccgacatta cccgatgcac
     4981 tgctattata ctgcgcatta ctggaacaac cagccagcag cacgattaat gccaccaacg
     5041 cgctacgtac tttccatttt ttcataaccg ttccctctgc atttaattaa tgcagcgatt
     5101 gtagggagat tatttaataa gaaaagcagc taaattattt ttaagaatat ataaaaagaa
     5161 agcgggccga tttacgtaaa taaatcgccc cgccagcaga ataatcttaa ttattcatct
     5221 tcaaga
//
LOCUS       DAWDRC010000254         5223 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_6999_length_5223_cov_2.436146, whole genome shotgun sequence.
ACCESSION   DAWDRC010000254 DAWDRC010000000
VERSION     DAWDRC010000254.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6999_length_5223_cov_2.436146"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3237..3246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4909..4918
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1212 a         1158 c         1371 g         1462 t
ORIGIN      
        1 gctggaaaat catactggtg ttcgcctgct ggacagaacc acgcgtgaag tggtgcttac
       61 agatgcaggg cagcagttgg ctttgcgtct tgagcgactg ctggatgaac ttaacagcac
      121 gttgcgcgat accgggcgta tggggcaaca actgagcgga aaagttcggg tcgctgccag
      181 ccagaccatt tccgcacatc ttattccgca atgcattgct gaaagccatc gccgctatcc
      241 agatattcag tttgtcctgc acgatcgtcc gcagcagtgg gtgatggaaa gtattcgcca
      301 gggagatgtc gattttggca tcgtcatcga tccaggccca gtgggcgatc tgcaatgtga
      361 agcgattctt tccgagcctt tctttctgct ttgccatcgc gatagcgctt tggccgtgga
      421 agattatgtc ccctggcagg cattacaggg agcgaaattg gtgttgcagg attacgcgtc
      481 aggcagccga ccgctgattg acgcagcgct ggcgcgcaac ggtattcagg cgaatattgt
      541 gcaggagatt ggtcatccgg cgacgctgtt cccgatggta gccgcaggca tcggcatcag
      601 tattcttccc gcactggcgc taccattgcc tgaaggtagc ccactggtgg taaagcgcat
      661 tacgccagtg gttgaacgcc agttgatgct ggtgcgcagg aagaaccgtt cactttccac
      721 cgccgcacaa gcgttatggg acgtagtacg agaccagggc aatgcgctaa tggctgggcg
      781 ggaaggggat ccgttgtatc agatatagag tgaattcata ctgccgggag ctccggcagt
      841 aatcaatcag aaattaaatt tgatggagta gattatcgca tcctggtaaa aatcttcgcc
      901 cagtttgtca tccgcataac gatactgaat ccctgtggtg atgtgtgaag ttgcattcca
      961 ccaaagtgcg acggcaccgt tcagaccttc tttgccgcca ttacctgccg cgtaggtggc
     1021 atcacgatca aactcgtact cgttccagtt ggtgagggtg aatttctcac tgcccagcat
     1081 aaagctgtaa cctgcaaccc aaccggcgac ataaccgtta tcgccggtat aataggtttg
     1141 gtctgtatag cgttttgcaa agaacggttt gaaccaccaa ccgctgccgg tgaaattgta
     1201 gccgatcccg taaaggaaca tatcgtcatg gaaattcata cgattcgcag aaccgtaggt
     1261 gccatacgca tgcaagtaga gattaaatcc ggtgtcaccc aggtaaatac ggttggtatt
     1321 tttaaatgta taacgctgct cgctacccgg tttatcttga cgatcattat aaaagttttc
     1381 ccagtcgaag aacccgtaca tttctcccca actaaagtta gcgccgcctt ccagttccag
     1441 atatccaaaa tcatctttgt gtgatttggt cgacgatttc tcagtggttc tgctagtcca
     1501 gtcaagataa tgaataccta tatcagcaaa accgccttta aattctgcat gggatactgc
     1561 tggtatcgcc aatacagaag agagtgtcag agtaaaaaga tgttttttca tagcataatt
     1621 ccctatgccg atcgttatta gttgattgct taataaaaag gttgattaaa agcggcatat
     1681 tgttaatgca agggaaagga gagtaccaat acagtttttt cagcctatta atagtgaaaa
     1741 aatataaata tatcgatatc gaataactat cggatacatg aaaaaagtgt attggtatga
     1801 ggtagggcaa tctcgttata aaaaggcgtt agattccacc ctacaaaaaa aggatatata
     1861 tgtatcaggc tcagttttct cataacccac tgtattgcgt agatattatc aagacttata
     1921 aacctgattt cacgccacga gtggccttta ttttaggttc cgggctgggc gcgctggccg
     1981 atcagattga gaacgcggtc gcaatttcct acgaaaagct gcctgggttc ccggtaagta
     2041 ccgtacacgg tcatgcgggt gagctggtgc tgggttatct ccagggggtg ccagtggcgt
     2101 gcatgaaagg tcgcggacat ttctacgaag gtcgtgggat gaccatcatg acggatgcaa
     2161 tccgtacctt taagttgctg ggctgcgagt tgctgttctg caccaatgcg gctggctcac
     2221 tgcgtcctga agtgggggcc ggcagtctgg tcgcattgaa agatcacatc aacaccatgc
     2281 cgggaacgcc tatggtgggt cttaatgatg aacgttttgg tgagcgcttc ttctcgctgg
     2341 cgaatgccta cgatgcggaa taccgcgcac tgttacaaaa agtggcgaaa gaagaggggt
     2401 tccctctgac ggagggcgtg ttcgtctcgt atccggggcc gaatttcgag actgcggcgg
     2461 aaattcgcat gatgcaaatt attggtgggg atgttgttgg tatgtctgtg gtgcctgagg
     2521 ttatttcagc tcgccattgc gaacttaaag tcgttgcggt ctctgcgatt accaacatgg
     2581 cggaaggtct gagtgacgtg aagctttctc atgcccaaac gctggcagca gcggaactct
     2641 caaagcaaaa ctttattaat cttatttgcg gctttctgcg caaaattgcc tgagtaaaac
     2701 aacaataaac ggccttactg agtgaggcca tatgatgcga caaggaaacg attatgagca
     2761 tcgctttgcg cttaaaggta atgtcctttt tgcaatattt tatctggggg agctggctgg
     2821 ttaccctcgg ttcttacatg attaataccc ttcatttcac cggcgctaat gttggcatgg
     2881 tttacagttc caaagggatc gccgcgatta ttatgcctgg tataatgggg atcatcgcag
     2941 acaaatggct gcgtgcagaa cgtgcataca tgctgtgtca cctggtgtgt gcgggcgtac
     3001 ttttttatgc ggcatccgta actgatccgg atatgatgtt ttgggtgatg ttagtcaatg
     3061 cgatggcgtt tatgccgact attgcgttat cgaacagcgt ctcttattcc tgtcttgccc
     3121 aggcagggct tgacccggtg accgctttcc cgcccattcg cgtttttggt acggtggggt
     3181 tcattgtcgc catgtgggca gtaagcctgc tgcatctgga attgagtagt ctgcaannnn
     3241 nnnnnncaac tgtatatcgc gtccggtgcg tcattgctgc tgtcggctta tgcgctgact
     3301 ttgccgaaga ttccggttgc ggagaaaaaa gcgactacat cgcttgccag caagctgggt
     3361 ctggatgcct tcgtgctgtt taaaaatcca cgcatggcta tctttttcct cttcgccatg
     3421 atgctgggtg cggtgctgca aattaccaac gtttttggca acccgttcct gcatgatttc
     3481 gcccgtaatc ctgagtttgc tgatagcttc gtggtgaaat acccgtccat tttactgtca
     3541 gtttcacaga tggctgaagt cggtttcatt ctgacgatac cgttcttttt gaagcgtttc
     3601 ggtattaaaa ccgtcatgct gatgagtatg gtggcgtgga cgctgcgctt tggcttcttt
     3661 gcctatggcg atccatcacc aaccgggttt attttgctgt tgctgtcgat gattgtttac
     3721 ggttgtgcat tcgatttctt caatatttct ggttcggtct ttgtcgaaca ggaagttgat
     3781 tccagcattc gtgccagcgc ccagggcctt tttatgacga tggtaaatgg cgtcggcgca
     3841 tgggttggct cgattctgag cggtatggca gtggattact tctcggtgga tggcgtaaaa
     3901 gattggcata ccatttggct gatgtttgca gggtatgctc ttttcctcgc agtgatattt
     3961 ttctttgggt ttaaatataa ccatgaccct gaaaagataa cgcatcgttc agttacacat
     4021 taaaaggatt cgcagctctg ttccttgggg ctgcttttat gataaaggtt aattagtgaa
     4081 agatatttta ttttcttctg gcgttggttt tggcattggt gctttgttta caatcgtgcg
     4141 attacctatt cctgttccaa atgtattgcc gggtatatta tccatcgtgt ttatgtatgt
     4201 cggatatctg gtggtgaaat actttatgcc atgataattt aatacgatat attttttata
     4261 tggagcactt aattatggaa cgcgtatata gaacagatct taagttgctc cgttattttc
     4321 ttgccgtagc ggaagagttg cattttggtc gcgcagcagc gcgtttaaat atgtctcagc
     4381 ctccgctcag tattcatatt aaagagctgg aaaatcaact cggcacgcag ctttttattc
     4441 gccattcgcg cagcgtcgta ctgacacacg cgggcaaaat cttgatggaa gaatcgcgtc
     4501 gaatgccgcc cgtgtacact gactgggatt ttttgcagcg agtttgccag caggtgggat
     4561 tttcaccggt tgttattcgc gaagttaatg aaccgcaaac ggtactcgcc atggtcagta
     4621 tgggtattgg tatcacattg atagcggaca gctacgcgca gatgaactgg cccggtgtca
     4681 tattccgtcc gctcaaagaa cgcatcccgg cagacttata tattgtttat gaaacgcagc
     4741 aggtgacgcc cgcgctggtt aaactgctgg cggcgttggc gcagtagatg agaggcacgg
     4801 gaactgccgg acatagttgg tctgataagc gaagcgcatc aggcaatttc gcaaattgca
     4861 gatagcaaaa aagcgccttt agggcgcttt tttgcattgg tgggtcgtnn nnnnnnnncg
     4921 gggttgtggg aacggcaatg tggggacgga tgcgcccggt tatgcggcga ttccttagag
     4981 aaaatcctaa cgttgaagtt ctttttcgcg aaaagatgcc cgcgatgcaa atggccttgc
     5041 tggaacgccg cgaacttgat gccgggatct ggcgaatggc gacagaacca ccgactggtt
     5101 ttaccagctt acggttgcat gaatcggcgt ttctggtggc gatgcctgaa gagcatcatc
     5161 tctcatcatt ttccaccgtc ccgctggaag cgctacgtga cgagtatttc gttacaatgc
     5221 cgc
//
LOCUS       DAWDRC010000255         5219 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7008_length_5219_cov_2.607475, whole genome shotgun sequence.
ACCESSION   DAWDRC010000255 DAWDRC010000000
VERSION     DAWDRC010000255.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7008_length_5219_cov_2.607475"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4314..4323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1304 a         1426 c         1264 g         1215 t
ORIGIN      
        1 ttggcggatc atcaatatta ctttccgggt tatcacatga ataaaaagca ctggatttca
       61 gtgtatcccg gcgaggaaat ctccgaagcg ttacttcgcg atctgatcaa cgattcgtgg
      121 aatctggtgg ttgatggtct ggctaaacgc gatcaaaaac gactgcgtcc tgactcaacc
      181 gtaaatcaat agcgcatttt tctcgcttac catttcatct cgatccttgt aatctgctgg
      241 cacgcaaaat tactttcaca tggagtcttt atggatatca tttctgtcgc cttaaagcgt
      301 cattccacta aggcatttga tgccagcaaa aaacttaccc cggaacaggc cgagcagatc
      361 aaaactctac tgcaatacag cccatccagc accaactccc agccgtggca ttttattgtt
      421 gccagcacgg aagaaggtaa agcgcgtgtt gccaaatccg ctgccgataa ttacgtgttc
      481 aacgagcgta aaatgcttga tgcctcgcac gtcgtggtgt tctgtgcaaa aaccgcgatg
      541 gacgatgcct ggctgaagct ggttgttgac caggaagatg ccgatggccg ctttgccacg
      601 ccggaagcga aagccgcgaa cgataaaggt cgcaagttct tcgccgatat gcaccgtaaa
      661 gatctgcatg atgatgcaga gtggatggca aaacaggttt atctcaacgt cggtaacttc
      721 ctgctgggcg tggcggcgct gggtctggac gcggtgccca tcgaaggttt tgacgccgcc
      781 atcctcgatg aagaatttgg tctgaaagag aaaggctaca ccagtctggt ggttgttccg
      841 gtgggtcatc acagcgttga agattttaac gctacgctgc cgaaatctcg tctgccgcag
      901 aacattacct taaccgaagt gtaattctcc cttgccgggc atctgcccgg ctatttcccc
      961 tcagattctc ctgatttgca taaccctgtt tcagccgtca tcataggctg ctgttgtata
     1021 aaggagacgt tatgcaggat ttaatatccc aggttgaaga tttagcgggt attgagattg
     1081 atcacaccac ctcgatggtg atgattttcg gtattatttt tctgaccgcc gtcgtggtgc
     1141 atattttttt gcactgggtg gtactgcgga ccttcgaaaa acgcgccatc gccagttcac
     1201 ggctttggtt gcaaatcatt acccagaata aactcttcca ccgtttagct tttaccttgc
     1261 aggggattat cgtcaatatt caggcggtat tctggctaca aaaaggcacc gaagcggcag
     1321 atattctgac tacctgcgcg cagttgtgga tcatgatgta tgcgctgctt tcagtcttct
     1381 cgttgctgga tgtgattttg aatctggcgc agaaattccc ggcggcatct cagttaccgc
     1441 tgaaagggat ttttcagggg attaaactga tcggcgcgat tctggtcggc attttgatga
     1501 tctcgctgct gattggtcag tcgccagcga ttctgatcag cggtcttggt gcaatggctg
     1561 ccgtgctgat gttggtattt aaagatccga ttcttggtct ggtggcaggt attcagcttt
     1621 ccgcgaacga tatgctgaaa ctgggcgact ggctggagat gccgaaatac ggcgcggatg
     1681 gcgcggtgat cgatattggg ttaaccaccg tcaaagtacg taactgggac aataccatta
     1741 ccactattcc cacctggtct ctggtttctg actcctttaa aaactggagc gggatgtcag
     1801 catctggcgg gcgacgtatt aagcgcagta tcagtattga tgtcaccagt attcgttttc
     1861 ttgatgaaga cgaaatgcaa cgtctgaata aagcgcattt gttaaagcct tatttaacca
     1921 gccgccatca ggaaattaat gagtggaatc gccagcaagg ttctacggag tcggtattaa
     1981 acctgcgccg aatgaccaat attggaacct ttcgtgccta tctgaacgaa tatctgcgga
     2041 atcatccgcg gattcgcaaa gatatgacct taatggtacg ccaactggct cctggtgata
     2101 acggtttacc gcttgagatc tatgcgttta ccaacaccgt ggtgtggctg gaatatgaaa
     2161 gcattcaggc tgatatattc gatcacatat ttgcgattgt cgaagagttt ggtctgcgac
     2221 ttcatcagtc gccaaccggc aatgatattc gctctctggc gggtgcattt aagcagtaat
     2281 taaaaaaacc gctctcatcg aatggatgag agcggtttcg gatggttgac atcattttgt
     2341 cggatgcggc gtgaacgcct tatccgacct acagaatcgt tacaaattgt aggcccgata
     2401 agcttgcgca tcgggcaaat cagcctcaca tcagcttaat tcactttcct acgcaatgtt
     2461 ttaaacgcca caaacaggaa cacaatccac accggcagca gaatcgctga caagcgcata
     2521 tcatccatcg tgcacatcag cagcaaaatc atgccgagga aggcgatgca aagatagttg
     2581 ccgaacggat agagcagcgc cttaaactgt gtttcacgcc cctgacgacg catcgctgca
     2641 cgaaaacgca gatgcgccag gcagatcata atccagttca acagcagcgt tgctaccacc
     2701 agcgccatca gcagaccaaa cgctttttgc ggcagcagat agttgattaa caccaccagc
     2761 gaagtgatcg ctccggaaag catcagcgag ttaatcggca cgccgcgacg gctgacgcga
     2821 gtcaaaaact ttggcgcatt accctgcaca gaaaggccaa acagcatgcg gctgttagag
     2881 taaaccccgc tgttatacac cgacagcgat gccaccagaa tgacgaagtt cagcgcagaa
     2941 gctaccacgt tgctgtcgag attatggaaa atcatcacaa acgggctact gttggatttc
     3001 acttccaccc acgggtagag cgccagtaaa accaccagtg aaccgatata aaacagcagg
     3061 atgcgataca ccacctgatt cactgcttta gggatgcttt tttccggatc gcgcgcttca
     3121 gccgcagtaa tcccaatcag ctccagaccg ccgaaggaga acataattac cgccagcgac
     3181 aaaatcaatc cattccagcc ggtggcaaag aaaccgccgt agcgccagag gttatcgata
     3241 ctggctttct caccgccgtg cccagaaaac agcagccaca ggccaaagcc gatcataccg
     3301 atgatcgcca gcactttaat cagcgcaaac cagaactcgg tttcgccata taagcgcacg
     3361 ttcaccaggt taacggcgtt gatgataata aagaaggcgg cagcccaaat ccacgttgga
     3421 acatccggga accagtactg catatagatg cccgcagcgg tcagctctgc cattcccacc
     3481 agcacgaaca ttacccagta gttccagcca gagaggaagc ccgcaaacgg tccccagtat
     3541 ttataggcaa agtgggcaaa tgaaccggat accggctctt cgaccaccat ctcgccgagc
     3601 tggcgcataa tcaggaaagc gatgatcccg gcgacgccgt agcccagcaa tacagccgga
     3661 cccgccatct gaatcgccgg gccaatgcca agaaacagac cagtaccaat tgcgccaccc
     3721 agcgcaatca gttgaatatg acggttatgt aatccgcgat gaagcgtcgg ctcttgattc
     3781 gacgcagtat cttccgatac ggttgacgcg tttttcacgc ctttcccctg tgtgtctttt
     3841 ttgttgaggg gcacctttta acatttagtg cccatcgtcg caagacacaa tccacacggt
     3901 taaacggggt atcctgcttt tatttccgta cccgatgtcg gtgcagccac atcagcttat
     3961 acgccgccgg gataataaac agcgacagca aaggcgcggt gatcatgccg ccaatcatcg
     4021 gcgcggcaat ccggctcatc acctctgacc ctgctcctgt tccccacaga atcggcagca
     4081 gaccagcgat aatcaccgcc accgtcatcg cttttgggcg cacgcgcagt accgcgccgt
     4141 gatataacgc ctcatccagc ttctgctcgc tgaatgtttg cggattattc aacgacggct
     4201 cggcctctat ggcgtgacgt aaatacatca gcatcaccac gccaaattcg gcggcgaccc
     4261 cggcaagggc aataaaaccg gtgcccgtcg ccacggaaag atgaaagccc atcnnnnnnn
     4321 nnnccaccac agcaaccaga tgccgcccac cagcgcaaac ggtacgctgc tgataatcag
     4381 caacgcttcg cccacccgac ggaacgccag atacaacagc acgaagatga tcatcagcgt
     4441 catcggcacc atcagtttca gcttatggtt ggcgcgctcc agtagctcga actgcccgga
     4501 gaatgccacg ctggtgcccg gttttaactg cactttttca gctatcgctt tttgcagatc
     4561 gtgaaccacc gacaccatgt cacgatcgcg ggcatcgata taaatccagc tcgtcgggcg
     4621 cgcattctcg gttttcagca tcgacggtcc ggtagagact ttaacgtcgg ccacgtccgc
     4681 cagagtgatt tgctgcttca tcggcgtgag gatcggcaac tggcgcagtg cctgcgggct
     4741 atcgcgccag ctttgcggat aacgcagatt aattggataa cgagcaatcc cttccaccgt
     4801 ttcgccaacc atcgccccgc ccaccgcaga agtcacaaac aactgcacat ccgccaccgt
     4861 cataccgtaa cgcgcggctt tttcacggtt aatctcaacg ttgatatagc gcccaccttc
     4921 cagacgctca gcaagcgcag aagccacgcc tggcaccgtt cgcgccactt cttcaatttg
     4981 ctccgccatc gtgtcgatat ccgccagcac agtgccggaa actttaatgc cgatagggct
     5041 tttaatgcca gtcgagagca tatcgatacg gttacgaatt ggcggcaccc acagattcgc
     5101 cagccccggc agacgcacgg tgttatccag ttcctcaatg attttgtcca tcgtcatgcc
     5161 aggccgccac tgatcctgcg gcttaagctg gatagtcgtt tctaccattt ccagcggag
//
LOCUS       DAWDRC010000256         5219 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7009_length_5219_cov_2.583269, whole genome shotgun sequence.
ACCESSION   DAWDRC010000256 DAWDRC010000000
VERSION     DAWDRC010000256.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5219)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7009_length_5219_cov_2.583269"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    424..433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1562 a         1134 c         1121 g         1392 t
ORIGIN      
        1 gtatgaccgt gaaatataat catggtcaat gtttaattaa taacattatt tgttactaag
       61 atcacacaat acttttctga actatgctta cataaaataa gacacgatgt gccttttatt
      121 taaataattc aacaaatgta acacacccta tccaggaggg atgatgaaaa ctgtatttac
      181 aacattatca gtcatattcg gcgtgatatt ttctcacagt gttctagctc aggacgttac
      241 tatttattat acgaatgaca ttcacgcaca tgtcaatccg gcaaaaatca tcgctgttga
      301 taagaacaga cttataggtg gtatggctaa tattgcaggc atcgttaatg aagcgaagaa
      361 aaaaagcaaa gatgtgtttt tctttgatgc aggcgactat tttaccgggc cgtatatcag
      421 cacnnnnnnn nnntatcatg aacactatgc cctttgatgc ggtgtctgtt ggtaatcacg
      481 agttcgatca cggtgtgccc aatatggtgt ctcagttatc aaaagcaaaa ttccccattc
      541 tgctgggaaa tatctactac accgatacga ataaaccagt ttgggatcat ccgtggacca
      601 ttatcgaaaa agatggttta aagattggtg tgattggatt gcatggcgca ttcgctttct
      661 atgatacagt cgcggcaaaa gcacgtgaag gcgtagaagc cagagacgaa attaaatatt
      721 taaataaagc acttgcagaa ttaaaaggta aagtcgacat aaccgttttg ctaattcacg
      781 aaggtgtccc ggcgcgtcaa tcaagctttg gtagtaaaga tgtggagcgg ctactacagg
      841 cagatattga gacagctaaa aaagtgaatg gtgttgatgt cttaattacc ggtcacgcac
      901 atgtcggaac accgcaacca ataaaagtta ataacacatt aattgtatct accgatgcat
      961 acggcaccaa catcggaaaa cttgtgcttg attacaatcc gaaaacgaag aaaattgata
     1021 gttataatgg tgagttaatc accatctttg cagatcaatt taaacctgac accatcgttc
     1081 aaaatactat tgataaatgg agtgcaaagc ttaacaaaat aacacaggaa gttgtcggcc
     1141 attctcccgt agttttaaca cgtgagtatg gtagttcctc ttctaccggc aaccttattc
     1201 ttgatgcaat gatggaaaaa acacctgatg ccgttgccgg atttcaaaat agcggtggga
     1261 tgcgagctga ttttcctaaa ggcgatatca cactgggaga tgttattagc acattcccct
     1321 ttaataatga cctcatcgag atggatttga cgggccgcga tctcaaatca ttgatgacgc
     1381 atgcaaccaa tctaactaat ggtgtgttac aggtttcaaa aagcgttgcg gttgtctatg
     1441 acagcaaaaa accactcaac caacggttaa tctctttcac cattaacggc aaacccgtgg
     1501 aagataatca aacatatcgt attgccacgc actccttttg tgccagtggt ggtgacggtt
     1561 ttgaagcatt tttgaatgga aaaaatgtga agacgatacc gggaacaacc tcggcggaat
     1621 ctatcatcga ttatttcaaa aatcataaac ctgtcacccc agacttaact aaacgagtca
     1681 tggacgtcgc caaataaaat ctcatttatc agtcaattta tttaacaagg aatgcgaatg
     1741 aaaacagcgc agcgtacttt acttgccgca acattgctga ctattttttc gacctcagtc
     1801 atggcgcagg atgtcacgat tatttatacc aatgatcttc acgctcatgt ggattcctat
     1861 aaagtaccgt atgtcgcaga cggaaaacgc gacattggtg gctttgccaa tatatccacg
     1921 ttagtaaaac aagagaaagc caaaaataaa gccacctttt attttgatgc tggagactat
     1981 ttcaccgggc catatatcag tagcctgaca aagggcgaag caataattga tattatgaat
     2041 accatgcctt ttgacgcagt atcaatcggt aatcatgagt ttgaccatgg ctgggataat
     2101 gccctccgac aattaagcaa agcgaatttc cctgttttat taggcaatgt ttatcataaa
     2161 gagagtgaga aacccttctg gaacaagccc tacaccatcc tggaaaaaga tggcatcaag
     2221 attggtattg ttgggctaca cggcgtattt gcatttaatg acactgtctc ggagctctct
     2281 ctccagggac tcgataatga caataataac cgatttgata aatcttccgc gaccctcaag
     2341 aatcagggga ttgaagcacg tgatgaagtg aagtatttgc agcattatat cgacgagtta
     2401 cgtgataagg ttgacctcac cgtggcgctg gttcatgaag gcgtaccggc gcgtcagtcc
     2461 agcattggca atacagatgt tagacgtgcg ctggataaag atattcaaac cgcaagcaag
     2521 gtgaaaggat tggatattct gattaccggg catgcccacg tcggaacgcc agaaccaatc
     2581 aaagtcggca acaccttaat cctttcgact gacagtggcg gtatcgatat tggtaaatta
     2641 atccttgatg tcaaccctac agctcgtact cataaagtga agagctttga gctgaaaacg
     2701 gtttatgccg atgaatggat acccgatcca accacacaaa aagttatcaa tggctggaat
     2761 aaaaaactgg tggatattgt acgccaacca gtcggtgaat catcgattgc attaactcgt
     2821 gcctacggtg agtcatcgca acttggcaac ctgtttaccg atgcaatgct tgtcgctgca
     2881 ccgactgcac aaattgcgtt gataaactcc ggcagtttac gtgctgatat aaatgcaggc
     2941 acgattacct ttggtgacat taccagtaca ttccccttca agaatgaact tactgaaatg
     3001 gatctcagcg gtaaggatct gcgtaaccta ctggagcacg gggcatcgct taccaatggc
     3061 atcctgcaaa tgtcaaaagg cgcagaaatg cgctataccc tgcagaaacc agtaggtcag
     3121 cgcattgtat cttttaaaat taatggtgaa gagatcgttg atacgaatat ttaccatgtt
     3181 gcgactacga ctttcctagc acttgggggc gatggttttc tggcattcaa agaagggaaa
     3241 aatgtccagg tccgcgccgg aaataacatg tccaatgtcg taatcgatta ttttaagaaa
     3301 ggccacaaga ttactcctgc acaggtaaat gaaatgcggg tagaagtaag caaataacaa
     3361 aaacttataa tgccattcat tcactggatg acattatttc tctctaaatc aacaatatat
     3421 aagagatata tttatgtata taaaaaagca ctggatagct ttatccattc tattaatacc
     3481 ttgcattgga aacgctcagg aaattaaaat tgatgaaagc tggttacatc aaagcttgaa
     3541 tgtcattggt cgcacagact ctcgctttgg cccaagactg actaacgacc tctaccctga
     3601 atatactgta gcaggaagaa aagactggtt tgatttttat ggttatgttg atctaccgaa
     3661 attctttggc gtcggcagtc actatgatgt tgggatctgg gatgagggct caccactatt
     3721 tacggaaata gaacctcggt tttccattga caaattgacc ggattaaatc ttgcgttcgg
     3781 cccatttaaa gaatggttta ttgcaaacaa ctatgtctat gatatgggtg acaaccagtc
     3841 atcccggcaa agtacatggt atatggggct tggtacagat atcgacacgg gtctaccaat
     3901 taagctttct gccaatatat acgccaagta tcagtggcaa aactatggtg ccgctaatga
     3961 aaatgaatgg gacggatatc gattcaaaat aaaatatagc atccctctta caaatttatt
     4021 cggaggacga ttggtataca atagttttac taactttgat tttggctccg atcttgcgga
     4081 caagtcacac aataataaac gaaccagtaa tgctattgct tcaagccata tcctttccct
     4141 tctatatgaa cactggaaat ttgcatttac actacgttat tttcacaacg gtggacaatg
     4201 gaatgcggga gagaaggtta acttcggaga tggtccattt gaattaaaaa atacaggatg
     4261 gggaacctat actactattg gttatcaatt ttaaattagg tgacatttta gatattgtta
     4321 cttaatgtca ttttgtaatt gtcattaata acttcaccat tcactctgtt acttcatcgc
     4381 tcaacacaaa agccccctta ttaccggggg cactgaccaa aggcaaaatg acaatcaatc
     4441 accacttccc caggaagagg tttaggggtg acaacaaaag ctggcaactg ccagcttttg
     4501 ttcaataatc actacaacaa ctgcgccagt cggttaatat ccgactgaat cgccccggct
     4561 gtgacgtcgc gccctgcacc tggcccgcgg atcaccagcg ggttgtcgcg ataccagcgg
     4621 ctttcgatgg caaagacgtt atcgcatggc agtagtgatg ccagcggatg gtcttcacgc
     4681 accgcttcca cgcccacacg cgctttgccg ttggcatcga aacgcgccac gtagcgcagc
     4741 accagcccca tttcgcgggc cgcttccagt cgttgcacca tctgctcgtt aagttcatcg
     4801 ccattttcaa agaaatggtc gatgctgccg ccttcgcaat gggcaggtac cagtgattca
     4861 acacgtacct ggtccgattc aatgttgtaa cccgcttcac gcgccagaat caccagcttg
     4921 cgcatcacgt ctttgccgga aaggtcatca cgcgggtcag gctcggttaa gccctgctgc
     4981 cacgcctgat ccaccagttc ggtaaacggc acgctaccgt cgaattgcag gaacagccag
     5041 gagagcgtgc cagagaagat cccactgatc gacaaaatgg tatcgccgct gtcgatcaga
     5101 tcgcgcacgg tatggttgat cggcaagccc gcaccgacgg tggcgttgta cagccagtga
     5161 cgcccggttt tttcgaaggc gtcgtggatc tggcgatatt tattgctgtc gcttgctcc
//
LOCUS       DAWDRC010000257         5210 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7023_length_5210_cov_2.831232, whole genome shotgun sequence.
ACCESSION   DAWDRC010000257 DAWDRC010000000
VERSION     DAWDRC010000257.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7023_length_5210_cov_2.831232"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1153 a         1335 c         1425 g         1297 t
ORIGIN      
        1 gaagtgttcg acgccagtac ggtatccggg cgtactttct gctcggtttc tgccagcacg
       61 gcttttttca ctttcgggtt ttcaacaacc gcttctacca caacatccac gcggtcaaac
      121 ccggcgtagt cgagcgttgg gtgaattgtg gagatcacgc cagccagttt cagaccatcg
      181 atcttgccgc gctcaagctg cttgttcagc agtttcgcgg cttcggtcat gccgagggtt
      241 aacgacttgt cgttgatatc tttcatgaca accggcacgc ctttccacgc agactggtag
      301 gcgatgccgc cgcccataat gcctgcgccc agcacagcgg cctgtttcgg ggtttcaaca
      361 tctttggtga gtttcttcgc tttacctttt acatattgat cgttaaggaa aatgccgacc
      421 agtgcgcggg cttcgttggt atgcgccagc gggacaaaac ttttgttttc caggtttaag
      481 gcttcttcac gaccaaaacg ggccgccgct tcaatggttt ttactgcggt gatgggggcc
      541 ggataatgtt tccccgccgt ttgcgcgacc atccctttag cgatggtgaa gctcatggcg
      601 gcttcaatct tgctcagttt tagcggttcc agcttcggct gacgttttgc tttccagtcg
      661 aggtcgccgt taatggcctg gcgtaaaatc gccatcgccc cttcaaccag tttttctgct
      721 ttgactacac catccaccag accgattttc agcgcctgat ccgcgccgac atctttaccg
      781 gcggcaatga tttccagcgc actgtcagcg cccagcatac gtggcatacg tacagaaccg
      841 ccaaagcccg gcatgatgcc cagtttggtt tccggcagac cgatgcgcag atccggcgtc
      901 gccagacgat aatcggtcgc cagcacgcat tcgcagccac cgcccagcgc atagccgttg
      961 acggcagcaa tggtcggcac cgggagatct tccaggcgat taaacacgct attggcaaag
     1021 tgcagccact gacttaactg ttcttcagga acgaggaaca gggacaaaaa ttcggtgata
     1081 tcagcaccga cgataaaggc tgctttgttc gaacgcagca gcagcccttt tagatctgat
     1141 tgctgttcca gcacgccgat ggcctcgccg aggctggcga cggtcgcagt gtcgagttta
     1201 ttaactgaac ctggggaatc aaataccagt tcggcaatgc catcttccag ccagtcaagg
     1261 tacagggtgt cgcctttgta aagcatgtca gtctcctgaa tccgcaaggt gatctggtcg
     1321 taccagatgc atcgaagtgt gtattttgtg ttaaaaatat gcaaataaaa tattaaggaa
     1381 atgccgatct gatcacgctc ggcagaaatc acgctctgga tgaacgatgt gctaatatgc
     1441 ggagacttaa ggtcaaaaaa acagaagggt aaaaaatgga atcactggcc tcgctctata
     1501 aaaatcatat agctacctta caggaacgga ctcgcgatgc gctggcgcgc ttcaagctgg
     1561 atgcgttact tattcactcc ggcgaactgt tcaacgtttt tctcgacgat catccctatc
     1621 cgtttaaagt gaacccgcaa ttcaaagcgt gggtgccagt aactcaggtg ccaaactgct
     1681 ggctgctggt ggatggcgtg aataagccga aactgtggtt ctatctaccg gttgattact
     1741 ggcacaacgt cgaaccgctg ccgaactcct tctggactga agatgtagaa gtgatcgcgc
     1801 tgccgaaagc cgatggcatt ggtagtctgt tgcctgctgc gcgcggcaat atcggttata
     1861 tcggtccggt gccagaacgt gcgctgcaac tgggtattga ggccagcaat atcaacccga
     1921 aaggggtgat cgactacctg cattactatc gctccttcaa aaccgagtac gaactggcct
     1981 gtatgcgtga agcgcagaaa atggcggtca acggtcatcg cgcggcagaa gaagcgttcc
     2041 gttctggcat gagcgagttc gatatcaaca ttgcctatct gaccgcgacc ggtcatcgtg
     2101 ataccgacgt accttatagc aacattgtgg cgcttaacga acacgctgcg gtgttgcatt
     2161 acaccaaact ggaccaccag gcaccggaag agatgcgcag cttcctgctg gatgccgggg
     2221 ccgaatataa cggctatgcg gctgatctaa cccgtacctg gtcggcaaaa agcgacaacg
     2281 actacgcaca gctggtgaaa gacgtaaatg atgaacaact ggcgctgatc gctaccatga
     2341 aagcaggcgt cagctatgtg gattaccaca tccagttcca tcagcgcatc gccaaactgc
     2401 tgcgtaaaca tcaaatcatc accgatatga gtgaagaagc gatggtcgaa aacgatctca
     2461 ccggaccgtt tatgccgcac ggtatcggcc atccgctggg cctgcaggtg catgacgtag
     2521 ccggttttat gcaggatgat agcggtacgc acctcgcggc accggcaaaa tatccgtacc
     2581 tgcgctgcac ccgtattctc cagccgggca tggtgttaac catcgaaccg ggtatctact
     2641 tcatcgaatc gcttctggca ccgtggcgtg aagggcagtt cagcaaacac ttcaactggc
     2701 agaaaattga agcactgaaa ccgttcggcg gcattcgtat cgaagacaac gtggtgattc
     2761 acgaaaacaa cgtggaaaac atgacccggg atctgaaact ggcgtgatgg aaagctggtt
     2821 aattcctgcg gcaccggtca cggtcgttga agagatcaaa aagagccgct tcattacgct
     2881 gttggcgcat accgatggcg tggaggcggc aaaagcgttt gttgaatcgg tgcgggcaga
     2941 gcaccccgat gcccgccacc attgcgtggc gtgggtggcg ggtgcgccgg atgattctca
     3001 acagctgggt ttctctgacg acggggagcc ggcgggaacg gcaggtaaac cgatgctcgc
     3061 ccaactaatg ggcagcggcg tcggggaaat taccgctgtg gtggtgcgct actacggcgg
     3121 catattgctt ggcaccggtg ggttagtgaa agcgtatggc ggcggcgtga atcaggcgct
     3181 gcgccagcta acgacccaac gcaagacacc attaaccgaa tatactttgc aatgtgaata
     3241 tagtcagtta accggcattg aagcgttgct ggggcagtgt gacggcaaaa ttattaacag
     3301 tgattatcag gcattcgttc tgctgcgggt ggcgcttccg gcggcgaaag tggctgaatt
     3361 ttccgcaaag ctggcggatt ttagccgtgg ttcattgcaa ttgttagcga ttgaagaata
     3421 atccccactt cgttttgcag aactaaggaa gcggcagaga tgcattttcg cgccattacc
     3481 cgaatcgttg gactactggt tatcttattt tcagggacca tgattatccc tgggctggta
     3541 gcactcatct accgggatgg agcgggccgc gcttttaccc agaccttttt tgtcgccctc
     3601 gccattggct ctatgctttg gtggccgaac cgcaaagaga agggcgaact gaaatcccgt
     3661 gaggggtttc tgatagtggt gctgttctgg accgtgctgg gtagcgtcgg tgcgctccct
     3721 tttatcttct cggaaagccc gaacctcacg attaccgatg cgttttttga atctttctct
     3781 ggcctgacca ccacgggggc cactacgctg gtggggctgg attcgctccc tcatgccatc
     3841 ctcttttatc gccagatgct gcaatggttt ggcgggatgg ggatcatcgt gctggcggtt
     3901 gcgatactgc ctatcctcgg cgtgggtggg atgcagctct atcgcgcaga aatgcccggc
     3961 ccgctgaaag ataacaaaat gcgcccgcga attgcggaaa cggcgaaaac cctgtggttg
     4021 atttatgtct tgctgaccgt cgcctgtgcg ctggcgttgt ggtttgctgg aatggatgcc
     4081 tttgatgcca tcggccatag ctttgcgact atcgctattg gcggcttctc gacacatgat
     4141 gccagtatcg gttatttcga cagcccgact attaacacta tcattgctat cttcctgctg
     4201 atctctggct gtaactacgg tctgcacttt tcactgttaa gtgggcgtag tctgaaggtt
     4261 tattggcgcg atccggaatt tcgcatgttt atcggcgtac agttttcact ggtggttatt
     4321 tgtacactcg tactgtggtt tcataatgtc tacagttcgg cgctgatgac aattaaccag
     4381 gcgtttttcc aggtggtgtc gatggcgaca accgccgggt ttacaactga cagcattgcc
     4441 cgctggccgc tctttttgcc ggtactgctt ttatgttcag cttttatcgg cggttgcgcc
     4501 gggtcaacgg gcggtggcct gaaagtgatc cgcatcctgc tgctgtttaa gcaggggaac
     4561 cgtgagctga aacgactggt gcatccgaac gccgtctata gcattaagct ggggaatcgc
     4621 gcactgccgg aacgtatcct cgaagccgtt tggggatttt tctccgccta tgcattggtg
     4681 tttattgtca gtatgctggc gattatcgcc acgggcgtgg atgacttttc tgcctttgcc
     4741 tcggttgttg caacattgaa taacctgggg ccagggcttg gcgtggttgc tgataacttt
     4801 accagtatga acccggtggc taaatggatc ctgattgcca acatgctgtt tggtcgtctc
     4861 gaggtcttta cattgctggt gctctttacc ccgactttct ggcgtgaatg atggagtaat
     4921 acgtgaaaac attaattctt ttctcaacaa gggacggaca aacgcgcgag attgcctcct
     4981 acctggcttc ggaactgaaa gaactgggga tccaggcgga tgtcgccaat gtgcaccgca
     5041 ttgaagaacc acaatgggaa aactatgacc gtgtggttat tggcgcttct attcgctatg
     5101 gtcactacca ttctgcgttc caggaatttg tcaaaaagca tgcgacgcgg ttgaattcga
     5161 tgccgagtgc cttttactcc gtaaatctgg tggcgcgtaa accggagaag
//
LOCUS       DAWDRC010000258         5210 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7024_length_5210_cov_2.181765, whole genome shotgun sequence.
ACCESSION   DAWDRC010000258 DAWDRC010000000
VERSION     DAWDRC010000258.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7024_length_5210_cov_2.181765"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1255 a         1225 c         1495 g         1235 t
ORIGIN      
        1 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
       61 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttgaaaaa
      121 taataacgtg atgggaagcg cctcgcttcc cgtgtatgat tgaacccgca tggctcccga
      181 aacattgagg gaagcgttga gggttcattt ttatattcag aaagagaata aacgtggcaa
      241 aaaacattca agccattcgc ggcatgaacg attacctgcc tggcgaaacg gccatctggc
      301 agcgcattga aggcacactg aaaaacgtgc tcggcagcta cggttacagt gaaatccgct
      361 tgccgattgt agagcagacc ccgctattca aacgtgcgat tggtgaagtc accgacgtgg
      421 ttgaaaaaga gatgtacacc tttgaggatc gcaatggcga cagcctgact ctgcgccctg
      481 aagggacggc gggctgtgta cgcgccggca tcgagcatgg tcttctgtac aatcaggaac
      541 agcgtctgtg gtatatcggg cctatgttcc gtcacgagcg tccgcagaaa gggcgttatc
      601 gtcagttcca tcagttgggc tgcgaagttt tcggtctgca aggtccggat atcgacgccg
      661 aactgattat gctcactgcc cgctggtggc gcgcgctggg tatttccgag cacgtaactc
      721 ttgagctgaa ctctatcggt tcgctggaag ctcgtgcgaa ttaccgcgat gcgctggtgg
      781 cattccttga gcagcataaa gaaaagctgg acgaagactg caaacgccgc atgtacacta
      841 acccgttgcg cgtactggat tcaaaaaatc cggaagtgca ggcgcttctc aacgacgctc
      901 cggcattagg cgattatctg gacgaggaat ctcgtgagca ctttgccggt ctgtgcaaac
      961 tgcttgagag cgcggggatc gcttacaccg taaaccagcg tctggtgcgc ggtctggatt
     1021 actataaccg taccgttttc gagtgggtga ctaacagcct cggttcccag ggcaccgtgt
     1081 gtgcaggtgg tcgttatgac ggtctggtgg aacaactggg tggtcctgca acaccggcag
     1141 tcggttttgc gatgggcctc gaacgtcttg tattgttagt tcaggccgtt aatccggaat
     1201 ttaaagccga tcctgttgtc gatatatacc tggtggcttc aggtgctgac acacaatctg
     1261 cggctatggc attagcagag cgtctgcgtg atgaattacc gggcgtgaaa ctgatgacca
     1321 accacggcgg cggcaacttt aagaaacagt ttgcccgtgc tgataaatgg ggtgcccgcg
     1381 ttgctgtggt gctgggtgag tctgaagtgg ctaacggcac agcagtagtg aaggatttgc
     1441 gctctggtga gcaaactgca gttgcgcagg atagcgtagc cgcgcatttg cgcacgttac
     1501 tgggttaagg aaggagaagg acagcgtgga aatttacgag aacgaaaacg accaggttga
     1561 agcggttaaa cgcttttttg ctgaaaatgg caaagcactg gctgttgggg tgattttggg
     1621 cgttggcgca ctgattggct ggcgctactg gaatagccat caggttgatt ctgcacgctc
     1681 cgcttctctt gcctatcaaa atgcggttac cgcagtgagc gaaggcaaac cggatagcat
     1741 cccagcggcg gaaaaatttg ctgctgaaaa taaaaatact tatggtgcgc tggcttcttt
     1801 ggaacttgcg cagcaatttg ttgacaaaaa tgaactagag aaagctgccg cccagttaca
     1861 acaggggctg gcagacacga gcgatgaaaa tctcaaagcc gtgataaatc tgcgtcttgc
     1921 tcgcgttcag gtacagctca agcaggctga tgccgcgcta aaaacccttg atgctatcaa
     1981 aggtgaaggg tgggctgcca ttgttgccga cctgcgtggt gaagcattgc tgagcaaagg
     2041 tgataagcaa ggtgcgcgta gtgcatggga agcaggcgta aaaagcgatg ttaccccggc
     2101 actgagcgaa atgatgcaga tgaaaattaa taatttgtcc atctgagagg gacccgatgc
     2161 aattgcgtaa attactgctg ccaggactgc tttccgttac ccttttaagc ggctgttcgc
     2221 tgtttaacag cgaagaagat gtggtaaaga tgtccccatt gccaaccgtt gaaaaccagt
     2281 ttacgccgac tactgcgtgg agcacttccg ttggtagcgg cattggcaac ttctattcca
     2341 atcttcatcc ggcactggcg gacaacgttg tctatgcagc ggaccgcgct ggtttagtaa
     2401 aagcgctgaa tgcggatgac ggcaaagaaa tctggtctgt caacctggcc gagaaagatg
     2461 gctggttctc taaagatcct gcattacttt ctggcggcgt gaccgtgtct ggtgggcatg
     2521 tctacattgg caccgaaaag gcgcaggttt acgcgctgaa taccagcgat ggtactgtgg
     2581 catggcaaac taaagtcgcg ggtgaagcac tttcgcgccc ggtggtcagc gacggtctgg
     2641 tgttaatcca caccagtaac ggtcagttac aagcgctgaa cgaagctgac ggcgctgtca
     2701 aatggacagt taacctcgat atgccttcgc tctctttgcg tggcgagtct gcgccggcaa
     2761 cggcttttgg tgcggccgtc gtgggtggcg ataatggtcg cgtcagcgca gtgctgatgg
     2821 aacagggcca gatgatttgg cagcagcgta tttcccaggc gaccggttct accgaaattg
     2881 accgtctgag cgatgttgac acgactcccg tcgttgttaa cggcgttgtt ttcgcgctgg
     2941 cctataatgg taacctgacg gcgcttgatc tgcgcagcgg tcagattatg tggaaacgcg
     3001 aactgggttc ggtgaatgat ttcatcgtcg acggcaatcg catctatctg gtcgatcaaa
     3061 atgaccgggt gatggcgttg accattgatg gcggcgttac gctgtggaca caaagcgatc
     3121 tgctgcatcg cctgctgact tctccggtgc tgtataatgg caacctggtg gtaggtgaca
     3181 gcgaaggtta tctgcactgg attaacgtcg aagatggtcg tttcgttgcc cagcaaaaag
     3241 ttgatagttc tggtttccag actgaaccgg ttgccgctga cggcaaactg ctgatccagg
     3301 caaaagacgg aaccgtgtac tctattacac gttaatcgtc tctgtcgttc actttgaaaa
     3361 cggctcctgg acaggggccg ttttcctgtt tttaacaacg acacgaatat agcgtgcgtt
     3421 gtctgatgat ttataaaaat gaggctttaa acatggtacc tgtggtcgcg cttgtcgggc
     3481 gccctaacgt aggaaaatcc acgttattta accgtctaac tcgcacccga gatgcgctgg
     3541 ttgcggattt cccgggtctg actcgtgacc gtaagtacgg tcgtgcagaa attgaaggcc
     3601 gcgagtttat ttgtattgat actggcggga ttgatggcac cgaagacggt gtagagacac
     3661 gtatggcgga acaatcgctg ctggcgattg aagaagcgga cgtcgtactg tttatggtgg
     3721 atgcgcgcgc gggcctgatg cctgcggatg aagcgattgc caaacatctg cgctcccgtg
     3781 aaaaaccgac cttcctggtg gcaaacaaaa ctgacggtct ggatcccgat caggcagtgg
     3841 ttgatttcta cgcgcttggt ttaggtgaaa tctacccgat cgccgcgtct cacggtcgtg
     3901 gcgtattaag tctgctggag cacgtgctgc tgccgtggat ggaagatctc gcaccgcaag
     3961 aagaagtcga cgaagacgcc gaatactggg cgcaatttga agcggaagag aacggcgaag
     4021 aagaagagga agacgacttc gacccgcaaa gtctgccgat caaactggcg attgtgggtc
     4081 gcccgaacgt aggtaagtct acactcacta accgtattct tggtgaagag cgcgttgttg
     4141 tttacgacat gcctggcacg acgcgtgaca gtatctacat tccaatggaa cgcgacggac
     4201 gtgagtatgt gctcattgac accgctggcg tacgtaaacg cggcaaaatc accgatgcgg
     4261 tagagaaatt ctcggtaatc aaaacgttgc aggccattga agatgccaac gtggtgatgt
     4321 tggtgattga tgcgcgcgaa ggtatttccg atcaggatct ctccttgctg ggctttattc
     4381 tcaatagtgg gcgctcactt gtgattgtgg tgaataagtg ggatggcctg agccaggaag
     4441 tgaaagagca ggtgaaagag acgctggact tccgtctggg ctttatcgat tttgctcgtg
     4501 tgcactttat ctctgccttg cacggcagtg gtgttggtaa cttgtttgaa tcagtacgtg
     4561 aagcgtatga cagctccacc cgtcgtgtgg ggacctctat gctgacgcgc atcatgacga
     4621 tggctgttga agatcaccaa ccaccgctgg tacgcggtcg tcgtgtgaag ctgaaatacg
     4681 cccacgccgg gggttataac ccgccgattg tggtgattca cggcaatcag gtgaaagacc
     4741 tgcctgattc ctacaagcgt tacctgatga actacttccg caaatcgctg gacgtaatgg
     4801 gatcgccgat tcgtattcag ttcaaagaag gggaaaaccc gtatgcgaac aagcgtaata
     4861 ccctgacgcc aacccagatg cgcaagcgta agcgcctgat gaagcatatc aagaaaagta
     4921 agtaatcact tatctggttt ccctttccac acggaaaggg aaaccgaata acgcgcgagg
     4981 agtatttatg gcactcaact gtccaacctg caaaacccct ctcgaacgca atggcgagac
     5041 tgcccactgt gcaacttgcg acaaagactt taccgtccag gcactttgcc ctgagtgtca
     5101 tcagccgtta caagtgttaa aagcttgtgg tgctgtggac tatttctgcc aacatgggca
     5161 cggtttaata tctaaaaaac gcgttgaatt tgttattgct taatatcact
//
LOCUS       DAWDRC010000259         5196 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7043_length_5196_cov_2.522077, whole genome shotgun sequence.
ACCESSION   DAWDRC010000259 DAWDRC010000000
VERSION     DAWDRC010000259.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7043_length_5196_cov_2.522077"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4427..4436
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1221 a         1271 c         1280 g         1414 t
ORIGIN      
        1 gtgggcgaaa tgtaactacg aagcccttga agaaacgggc aagctcggcg agatcctctt
       61 ctttatgcgc gccggttcca ccggtagcca aaaatattcc accatgatgt gggcgggcga
      121 ccagaacgtt gactggagtc tggacgatgg actggcgtct gtcgtcccgg cggcgctgtc
      181 gctggcaatg accggacatg gcctgcatca cagtgatatt ggcggttaca ccaccctgtt
      241 tgagatgaag cgcagcaaag agctgctgct gcgctggtgc gatttcagcg ccttcacgcc
      301 gatgatgcgc acccatgaag gtaaccgtcc tggcgacaac tggcagtttg acggcgacgc
      361 ggaaactatc gcccattttg cccgtatgac caccgtcttc actaccctga aaccttacct
      421 gaaagaggcc gtcgcgctga atgcgaagtc cggcctgccg gttatgcgcc cgctgttcct
      481 gcattacgaa gacgatgcac agacctacac cctgaaatat cagtacctgt taggccgcga
      541 cattctggtc gccccggtac acgaagaagg tcgcagcgac tggacgctct atctgccgga
      601 ggataactgg gtccatgcct ggacgggtga agcgttccgg ggcggggaag tgaccgttaa
      661 tgcgcccatc ggtaaaccgc cggtctttta tcgcgccgat agcgaatggg cggcactgtt
      721 cgcgtcgtta aaaagcatct aatcacatcc gcccgtgggg aaactcacgg gcaaagggag
      781 aacaacgatg agtcacatca caacggaaga tccagcaact ctacgcctgc cctttaaaga
      841 gaaactctct tacggtatcg gcgatctggc ctctaacatc ctactggata tcggcacgct
      901 ttatcttttg aagttttata ccgatgttct ggggctgcct ggcacctatg gcggcattat
      961 ctttttgatt tcaaaattct ttaccgcctt taccgatatg ggtaccggca ttatgttgga
     1021 ttcccgacgc aagatcggtc caaaaggtaa gttccgtcct tttattctgt atgcgtcatt
     1081 tccggtcacc ctgctggcga tcgccaactt tgtcggcaca ccgtttgatg tcaccggtaa
     1141 aacggtgatg gccaccatcc tgtttatgct ttacggactg tttttcagca tgatgaactg
     1201 ctcgtatggc gcgatggtcc ccgctattac taaaaacccc aacgaacgtg catcgctggc
     1261 agcatggcgt cagggtggcg ctacattggg cctgctgctg tgtacggtgg gattcgtgcc
     1321 ggttatgaat cttatcgagg gtaatcagca acttggctat atcttcgccg ccacgctgtt
     1381 ttcactgttc ggcctgctgt ttatgtggat ctgctattcg ggcgtgaaag agcgttatgt
     1441 cgaaacccaa cctaccaatc cggcgcaaaa gcctggcttg ttgcaatctt tccgcgcaat
     1501 tgcggggaac cgcccactgt tcattttgtg cattgccaac ctctgtacct taggggcgtt
     1561 taacgtcaag ctcgccattc aggtctatta cacccagtac gtgcttaacg atcccatcct
     1621 gttgtcatat atgggatttt tcagcatggg ctgtattttc atcggcgtgt tcctgatgcc
     1681 tggtgcagtc agacgttttg gtaagaagaa ggtctatatc ggcggtctgc tgatctgggt
     1741 gctgggcgat ctgctcaact atttcttcgg cggcggctcg gtcagctttg tggctttctc
     1801 ctgcctggcg ttctttggtt cggcgtttgt taacagcctg aactgggcgc tggtttctga
     1861 caccgtcgag tatggcgagt ggcgcaccgg tgtgcgttcg gaaggtacgg tctacaccgg
     1921 tttcaccttc tttcgcaaag tatctcaggc gctggcaggt ttcttcccag gctggatgct
     1981 gacgcaaatt ggctatgtgc caaacgtcgc ccaggctgac cacactatcg aagggttgcg
     2041 ccaactgatc ttcatctacc caagcgcact ggcagtagtc accattgtgg caatgggttg
     2101 cttctacagt ctgaacgaga agatgtacgt ccgcattgtt gaagagatag aagcccgtaa
     2161 acgcacggcg taattataat aaacaacgcc ctgcggggcg ttataaggag tgattatgtc
     2221 tgaccataat ccactgacat taaaactgaa tctgcgggaa aaaatcgcct atggtatggg
     2281 cgacgtcggt tcgaatttaa tgcttagcat cggcactctg tatctcctca aattttatac
     2341 cgatgaatta gggatgcctg cttactatgg cgggattatc tttctggtgg cgaagttttt
     2401 taccgcattt accgatatgc tcaccggatt tttactcgac tcgcggaaaa atattggacc
     2461 taaaggaaaa ttcaggccgt ttattttata cgcagcgatt ccggcggcgt taattgcaac
     2521 gctccagttt atcgccacca cctttagttt gccggttaaa acgacgattg ccaccgcact
     2581 gtttatgatg tttggacttt catacagtct gatgaactgc tcgtatggcg cgatgatccc
     2641 ggccattacc aaaaacccga atgagcgcgc gcaactcgct gcttaccgcc agggtggtgc
     2701 aaccataggg ctattgatct gtaccgtggc gtttattcct ttgcagtcac tattttctga
     2761 ctccaccgtc ggctacgcct gcgcggcact gatgttctcc attggcggct ttatttttat
     2821 gatgctgtgc tacagaggtg tcaaagagca ttatgtggac acgacgccaa ccggacataa
     2881 ggccagtatt ctcaaatcat tttgcgcgat atttcgtaat ccacctttgc tggtattgtg
     2941 cattgctaac ctgtgtaccc tggcggcatt taatatcaaa ctggcgattc aggtctatta
     3001 cacccagtat gtgctgaatg atattaactt attgtcgtgg atgggattct ttagtatggg
     3061 atgcattctc gtcggcgtat tactggtgcc actaactgta aaatgttttg gtaaaaaaca
     3121 ggtttatctg gctggcatgg tgctgtgggc ggtgggggat atcctgaatt attgctgggg
     3181 cagtaactca ttcactttcg tcatgttctc ttgtgtcgcc ttttttggca cggcctttgt
     3241 aaacagcctg aactgggcac tggtaccaga taccgtagat tatggtgaat ggaaaacagg
     3301 tatacgtgcc gaaggttctg tttataccgg ttataccttc tttcgtaaaa tttcagccgc
     3361 acttgctggc ttcttgcccg gcattatgct gacgcaaatc ggttatgttc ccaacatcgc
     3421 gcaaagcgat gcaacattac agggtttgcg tcagctcatt tttatctggc cttgtgcgct
     3481 ggcaattatt gctgcattga cgatggggtt cttttacacg ctcaatgaga aacggtttgc
     3541 actgattatt gaggaaatta accaacgcaa aaataaagaa attgagacag aagaaaaaac
     3601 ggcttccgta acgttataaa tgtcaatagc cgctatttcc atcctgatgg gtggcggcct
     3661 ccctacgttt aaaaaatgga cttattatga aaaatattaa tgccataatt ttattatctt
     3721 ctctgacctc tgcctcggta ttcgctggcg catacgtcga aaacagggaa gcatataatc
     3781 tcgcctccga tcagggtgag gttatgctac gtgtgggtta taacttcgat atgggcgcgg
     3841 gtattatgct aaccaatacc tacacctttc agcgagaaga tgaactcaaa catggctata
     3901 atgaaattga aggttggtat cctttattta aacctaccga taaattaacc atccagcccg
     3961 gtggcttaat taatgacaag agtatcggtt cgggtggtgc tgtttatctg gatgttaact
     4021 acaaatttac tccgtggttt aacctgacgg tgcgtaatcg ctataaccat aataattaca
     4081 gctctacaga tttgaacggc gaattggata ataacgacac ctatgaagtt ggcacctact
     4141 ggaactttaa aataacagaa aaattttcct atacatttga accacattac ttcatgcgag
     4201 tgaatgactt taatagtagc aacgggaaag atcatcattg ggaaatcact aacaccttcc
     4261 gttaccgtat taatgaacac tggcttcctt atttcgaatt gcgctggtta gaccgcaacg
     4321 tcgagccgta ccaccgcgag caaaaccaga ttcgtatcgg aacgaagtat ttcttctgat
     4381 gtaaaaattc ctgatgcgat gctggcgcat cttatcaggc ctacaannnn nnnnnnatta
     4441 atttcgtttt tcagtcataa aaaacctgcc atcgctggca ggttttttat gactaaaaaa
     4501 cgaaattaat cgtctttcgg tgcgcggttg gcgcgacgac gatcgttttc cgtcaggtga
     4561 cgtttacgaa tacgaataga ggtcggagtc acttctacca gttcgtcatc atcgatgaac
     4621 tccagagctt gttccagagt catgcggata ggcggaacca gaacaacggc ttcgtcagta
     4681 ccggaagcac gcatgttggt cagtttctta ccggtcaggc agtttacagt caggtcgtta
     4741 gagcggctgt gaataccgat aatctgacct tcgtaaactt ctgcaccgtg accgaggaac
     4801 agcttaccgc gatcctgcag accgaacagc gcgaacgcga ccgctttacc ctgaccgtta
     4861 gagatcagta cgccgttctg acgctgaccc acttcacccg gacgtacgtc gtcgtagtgg
     4921 ctgaaggtgg agtacagcag acccgtaccg gaagtcatgg tcatgaactc agaacggaag
     4981 ccaatcagac cacggcttgg gatcacgtag tcgagacgta cgcggccttt accgtctgga
     5041 ttcatgtttt tcaggtcgcc tttacgttcg cccagcgcct gcattacaga accctgatgc
     5101 tgttcttcaa cgtccagagt cacgttttca tacggctctt gtttacgacc gtcgatttca
     5161 cggaagataa ctttcggacg ggataccgcc agttcg
//
LOCUS       DAWDRC010000260         5181 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7064_length_5181_cov_2.036286, whole genome shotgun sequence.
ACCESSION   DAWDRC010000260 DAWDRC010000000
VERSION     DAWDRC010000260.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7064_length_5181_cov_2.036286"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1603..1612
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1255 a         1374 c         1356 g         1186 t
ORIGIN      
        1 ccgccataat cgcgtagttc gtggcgaggc cgtctatcgc tgtgttcact gcggcgaaca
       61 gctggttgcg aaataaccac ctgaactatc agaaactttc ctgatctggc tgattgcata
      121 ccaaaacaac tttcgctacg ttgctggctc gttttaacac ggagtaagtg atgtaccgtt
      181 atttgtctat tgctgcggtg gtactgagcg cagcattttc cggcccggca ttggccgaag
      241 gtatcaatag tttttctcaa gcgaaagccg cggcggtaaa agtccacgct gacgcgcccg
      301 gtacgtttta ttgcggatgt aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc
      361 aatcgtgcgg ctatcaggtg cgaaaaaatg aaaaccgtgc cagccgcgta gagtgggaac
      421 acgtcgttcc tgcctggcaa ttcggtcacc agcgccagtg ctggcaggac ggtggacgta
      481 aaaattgcgc caaagatccg gtctatcgca agatggaagg cgatatgcat aacctgcagc
      541 cgtcagtcgg tgaggtgaat ggcgatcgcg gcaactttat gtacagccag tggaatggcg
      601 gtgaaggcca gtacggtcaa tgcgccatga aggtcgattt caaagaaaaa gttgccgaac
      661 caccagcgcg tgcacgcggt gccattgcgc gcacctactt ctatatgcgt gaccaataca
      721 acctgacact ctctcgtcag caaacgcagc tgttcaacgc atgggacaag atgtatccag
      781 ttaccgactg ggagtgcgag cgtgatgaac gcatcgcgaa ggtgcagggc aatcataacc
      841 cgtatgtgca acgcgcttgc caggcgcgaa agagctaacc tacactagcg ggattctttt
      901 tgttaacccc taccccacgc gtacaaccgc gtggggagac gacgcggatt tttaactatg
      961 cgtatccccc gcatttatca tcctgaacca ctgaccagcc attctcacat cgcgctttgc
     1021 gaagatgccg ccaaccatat cgggcgcgta ctgcgtatgg ggccgggaca ggcgctgcaa
     1081 ttgtttgacg gcagcaacca ggtctttgac gccgaaatta ccagcgccag caaaaaaagc
     1141 gtggaagtga aggtgctgga aggccagatc gacgatcgcg aatctccgct gcatattcac
     1201 ctcggtcagg tgatgtcgcg tggtgaaaaa atggaattta ctatccagaa atcgatcgaa
     1261 ctcggtgtaa gcctcattac gccacttttt tctgagcgct gcggcgttaa actggatagt
     1321 gaacgtctga acaagaagct ccagcagtgg cagaagattg caattgctgc ctgtgagcag
     1381 tgtggccgta accgggtgcc ggaaatccgt ccagcgatgg atctggaagc ctggtgtgca
     1441 gagcaggatg aaggactgaa actgaatctt cacccgcgcg ccagtaacag catcaatacg
     1501 ttgccgttac cggttgaacg cgtccgcctg ctgattggcc cggaaggcgg tttatcggca
     1561 gatgaaattg ccatgactgc ccgctatcaa tttactgata tcnnnnnnnn nntcctgttg
     1621 ggacctcgcg ttttgcgtac tgagacaact gcgctcaccg ccattaccgc gctacaagtg
     1681 cgttttggcg atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac
     1741 cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag
     1801 cgtcgtggtt acgaacttca ctatatggag atggccgatc tctatttgat caatggtgag
     1861 gctcgcgctc gtacccgtac tttgagcgtc gaacagaact acgataaatg gtacgagttc
     1921 acgggtgagc aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg
     1981 ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg
     2041 acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgaaaaact gttcactgcc
     2101 tggttctctg acttaacgcc agaaacactg gtcacgcgca ataaagcgca gttgaaagcg
     2161 ttctgggaaa aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg
     2221 attttccgcg tgaaagaagg tgatccaaac ctcggcgtga ttgccgaaac cctgactgag
     2281 catggcactc gctactgcat ggcgcaaaat tacctgccag ccattaaaga tggcgacaaa
     2341 cgcgtgctgg tagtggatgg cgagccggtg ccgtattgcc tggcgcgtat tccgcagggg
     2401 ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa
     2461 agtgactgga aaatcgcccg tcagattggg ccgacgctga aagaaaaagg gctgattttt
     2521 gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt
     2581 attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc
     2641 gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga
     2701 gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca
     2761 gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat
     2821 ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg
     2881 atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag
     2941 atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggcggt
     3001 ccgctggctg aagatcgcgg gtttattttg catactccgc cctccaattt tgcttccagc
     3061 attcgcattt cagacaacac agtgatgacc acttcccgcg atgtgctgga aacgctcggc
     3121 accgataaac aaccgtctga cgtattggtg gctctgggtt atgcctcctg ggagaaaggt
     3181 caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcaga tctgaatatt
     3241 ctgttcaaaa cgccgattgc cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat
     3301 attctcacca tgcctggtgt ggcaggacac gcctgatgag tggaacctta ctcgccttcg
     3361 acttcggcac caaaagcatt ggcgtagcgg tcggccaacg cattaccggc accgctcgcc
     3421 ctttgcctgc aattaaagcg caggacggta cgccggactg gaacattatc gagcgtttac
     3481 tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg
     3541 aacagccgtt aaccgccaga gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg
     3601 ttgaagtaaa gctccatgac gagcgtctta gcaccgtgga ggcacgttcc ggtctgtttg
     3661 aacagggcgg ctatcgggcg ctcaacaaag gcaaagtcga ctctgcctct gcggttatta
     3721 ttctcgaaag ctatttcgag cagggatatt aaggcgattt actagcgcac agatttctga
     3781 caatgtgctc gttgtttcat gttggatgcg gcgtaaacgc cttatccaac ctacagtgag
     3841 tgcgaattca aagttacgct tcccttgtag gcccgataag cttgcgcatc gggcatattt
     3901 acaattcgta tcagacacag taacgtcaca agcgcccttc tttcacccgc tgctgataac
     3961 tctgctgaaa cgttatcatc cccacctgct gcccggtttg aataacatgc ggtaactggt
     4021 gggttttccc ttcacgaatc aaattcccca ccgcgggtgt gttaatcagt aattcaaaca
     4081 gcgccacgcg tccttcctgt ttatccactt ccagtttttg tgacaacact gcccgtaaac
     4141 tacctgccag ttgattacgc acgggatctt tttcctgcgc aggaaatgaa tccaccagtc
     4201 gctcaactgc ctgcgctgcg ccgcgcgtgt gtaatgtcgc cagcaccaga tgcccggttt
     4261 ctgccgccgt cagcgccaga cggattgtct cgctatcacg cagctctccg agcaaaatca
     4321 catcaggatc ttcccgcaat gcagcccgca atcccgatgt gaacgtcata cagttcaaac
     4381 caatctcccg ctgctggatc aaacatcgct gactggtata gagatattcc acaggatctt
     4441 ccagcgtcag aatatgcgca tcggcatgtt gattgagata gccaaccatc gccgccagcg
     4501 tggtagattt gccgctcccc gtcgcccccg tcaccagaat caggccattc tcgctcttga
     4561 gtaattccgg caataccggt ggtgcgccaa gctgttcgag ctgcgggcag tgcgaaggta
     4621 acagccgtaa cgccagagaa ataccttgcc gttgtgcgaa cgcactgccg cgcaatcgct
     4681 ggttttccgc cagcgacaca gcaaaatcca gctgaccatt ctccagcaat attgcccgct
     4741 gatcgtcatc cagccactcc cgcagtagct cttcgacgtc cggcgcatca aacggcgcag
     4801 cttccattct gccgcgtatg cgccatcgtg cgggccaggc gctgcacagg tgtagatccg
     4861 agacgttatg ctttacacta agggccacaa tttcttccat attcatacta agatcctcgg
     4921 aaaatgaacg atattgcgca taacctggca caggtccggg acaaaatctc agcggctgca
     4981 acgcgttgcg gccgttctcc agaagaaatt acgctgcttg cagtcagtaa aacaaaacct
     5041 gcgagcgcca tcgcagaagc cattgatgcc gggcagcgtc aatttggtga aaactacgtt
     5101 caggaagggg tagataaaat tcgccacttt caggaactgg gcgtaacagg attagaatgg
     5161 cattttattg gcccgttgca g
//
LOCUS       DAWDRC010000261         5181 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7063_length_5181_cov_2.165236, whole genome shotgun sequence.
ACCESSION   DAWDRC010000261 DAWDRC010000000
VERSION     DAWDRC010000261.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7063_length_5181_cov_2.165236"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4160..4169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4544..4553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1247 a         1396 c         1271 g         1247 t
ORIGIN      
        1 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
       61 catgtcgttg atcgcgttgt tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
      121 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
      181 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
      241 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
      301 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccccagggta
      361 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
      421 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
      481 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
      541 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
      601 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacacgccg
      661 cctggacgca aaactgacca cagggtctgt aatacgctgc ggggatcggt cacccactcg
      721 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
      781 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
      841 gcgcggtcga tcatctgcgc agaaagatcg cataaaatga cctgatgccc gcgctcggcc
      901 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
      961 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     1021 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     1081 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     1141 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     1201 caaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     1261 tgccacttcc gctgatgttg ttgattattg gcgctggcct ggcgctgctg tggtttagcc
     1321 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcgctc ttgttattga
     1381 gtttgcaacc gatagccgac cgcctgttgc gtcccatcga aagcacttat ccgacatgga
     1441 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     1501 agtgggcgcc gagctctaat ttaatcaata acagtctgcc acgcctgaat gaaggtattc
     1561 gcttatggcg cgaaaatccg ggatcaaaac tgatctttac gggaggcgta gctaaaacca
     1621 atacggtgag cacagccgaa gtaggtgcca gagttgcgca atcgctgggc gtgccgcgcg
     1681 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgca gcagtgaaac
     1741 aggcgattgg tgacgcacct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     1801 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     1861 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtttgg ttgatgcata
     1921 gcgaccgcgt tggctatgaa acgcttggcc ggatctggca gtggctgaaa ggttcgtcag
     1981 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tacgccgact aaatcgtccg
     2041 gtgttgacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     2101 gactccgcca ctggcgcgcg ttgcagataa ctccagagca aatcttccgc cagagaatta
     2161 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggtccgaca
     2221 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tacgtaaaca gaagttaccg
     2281 tgaatcagta cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     2341 cgaaacagga tccgcttatc ctgcatcgtt agaccggtgt tattgaactg attgagcgtg
     2401 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     2461 tcgaccgcgc cgacgcaacc acgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     2521 atttgatctt tgagttgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     2581 ccacgcattc gctccagtag caggacatcg gggccggggt gttcttcatg cgtcatcaca
     2641 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     2701 agttgtcggg cttttccggg cgcactaaag cttcttgcca ttaacggcat tgggtttccc
     2761 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     2821 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     2881 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     2941 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     3001 ggctggagtg tcgtgaagtg aaattagcgc atttcggcgc gaacgcgctt gagatcttca
     3061 ggggtatcca cacctgtgcc aggaacttcc tgggcgacag caacgtggat tttttcgccg
     3121 taccacagaa cacgaagctg ctctaacatt tcgatatgtt ctaacggact tggctgccag
     3181 gtaacataac gacggataaa gcctgctcgg tagccataaa taccaagatg acgcaggaag
     3241 ttatcgccta cggtttcaag gtcttttgca aaacgatcac gatcccacgg aatggtggcg
     3301 cgagagaagt acagtgcgta cccttcggcg tcgagaacca ctttcaccgc attcgggtta
     3361 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatccccac ctgacgctga
     3421 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atccccctgc
     3481 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     3541 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaaca
     3601 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcgcgc
     3661 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     3721 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
     3781 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcgcagcg
     3841 ggaaagccag gttgtccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
     3901 ttccgttgca aaccgggcag gcaatgattt caagcagacg atgatccata gttcctccag
     3961 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
     4021 cccagccggc gcggcaatca gtaaagaggc cttgcgggca gcgtcctaat gtcacccgcg
     4081 ttgcctgctg ccagttagcg aaatcagtaa tcgcctgacg taaccctttt tgcagcgttg
     4141 tcgttggttt aatgccttcn nnnnnnnnnc cacagagaga taacttcaag gatgcctgtc
     4201 tggcgatgca ttttggcatc cattcgccca actaattgcc cacgatgtaa taacggcaga
     4261 acaaaatagc catactggcg tttcggcgct gggatatagc actccagccg gtagctaaaa
     4321 tcaaaaagct gctccgcgcg tttgcgatcc cagacaacag gatcaaaagg cgaaagtacc
     4381 gcgctgtgcg ttgcagtgag ctttccggcg agcgcacgct cgagtagcgg cagcaaatca
     4441 gcatgcagcc aaagattgcc caatttttca acatgcacag caatgatttg ctgctgttca
     4501 gccctcgctt cgcgccaagc cgccagtgcc ggacgtttca gccnnnnnnn nnngttcgcg
     4561 gaatatgccc aggctacgcg cactgttatc cagcatgatg atttctgctt ctgtttgcga
     4621 aacgagatcg cgctcatcat cccagtcagg catgacacgg tgggttaaat cataaacgcg
     4681 ctggaagttg cgccgttcaa tcaccatcac ctttccggca gtaaataaac cttccagatg
     4741 ccgtttatgc ggcttccatt cccaccagcc gcttgcacct ttacgagaat gctcgaaatc
     4801 ggctgaacgt accggcccct tatcatgaat atgctgaatt aactgtgcaa tttccgccgc
     4861 atgttcctgc atccaggcgt ctttgtattt ccagcccatt ttttcaggtg ccagcatgcg
     4921 gtggcgaata agacgaaaat cgctacgcgg cataaagcag gcttcatgcg cccagtattc
     4981 cattaattcg ccacgcgcca gagactcatc cagccactgg gcaggataat ttcccagacg
     5041 actgaaaagc accagatatg ggctacgggc aacaatatta atggtatcga tttgcagcaa
     5101 ggacatgcgg gagatcgttg ccggaatatc ctccaacgac gctcgacggc ggggtttgtt
     5161 taacaggcct tgtgcagcga g
//
LOCUS       DAWDRC010000262         5180 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7065_length_5180_cov_2.072195, whole genome shotgun sequence.
ACCESSION   DAWDRC010000262 DAWDRC010000000
VERSION     DAWDRC010000262.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5180)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7065_length_5180_cov_2.072195"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1995..2004
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4354..4363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1225 a         1337 c         1313 g         1285 t
ORIGIN      
        1 ccacagcaat cctggccctg gcgctttccg caggactggc acaggcagat gacgccgccc
       61 cggcagcagg cagcacgctg gacaaaatcg ccaaaaacgg cgtgattgtc gtcggtcact
      121 gtgaatcttc agtacctttc tcttattacg acaatcagca aaaagtggtg ggctactcgc
      181 aggattactc caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccagacttac
      241 aggtgaaatt gattccgatc acctcacaaa accgtattcc actgctgcaa aacggcactt
      301 tcgattttga atgcggttct accaccaaca atgccgaacg ccaaaaacag gcggctttct
      361 ctgacactat tttcgtggtc ggtacgcgcc tgctgaccca aaagggcggc gatatcaaag
      421 attttgccga cctgaaaggc aaagccgtag tcgtcacttc cggcaccacc tctgaagttt
      481 tgctcaacaa actgaatgaa gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc
      541 acggtgactc tttccgtacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgatg
      601 ctctgctggc cggtgaacgt gcgaaagcga agaaaccaga caactgggaa atcgtcggca
      661 agccgcagtc tcaggaagcc tacggttgca tgttgcgtaa agacgatccg cagttcaaaa
      721 agctgatgga tgacaccatc gctcaggtgc agacctccgg tgaagcggaa aaatggtttg
      781 ataagtggtt caaaaatcca attccgccga aaaacctgaa catgaatttc gaactgtcag
      841 acgaaatgaa agcactgttc aaagaaccga atgacaaggc actgaactaa ttacaagaac
      901 caggggcgga aattccagcc ctctcgattg ttacgtagca cggacagact atacgcctgg
      961 tggtcgttcc ccatcgggcc tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag
     1021 cagttaacgc tgctaccctc ttttttctgg agtagattta tgtctataga ctggaactgg
     1081 ggtatttttt tacaacaagc cccgttcggc aacaccacct atcttggttg gatctggagt
     1141 ggctttcagg tgacgatcgc tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt
     1201 tcatttttcg gcattttacg taccgttcca aaccgttttc tctccggtct gggtacgttg
     1261 tatgtcgaac tgttccgcaa cgtgccgttg attgtgcaat tctttacctg gtacctggtg
     1321 atcccggagc tgctgccaga gaaaatcggc atgtggttta aggcagagct ggatcccaat
     1381 attcagtttt tcctttcatc catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc
     1441 gaacaggtac gcgccgctat tcagtcgctg ccgcgtgggc aaaaaaatgc cgcgctggcg
     1501 atgggtctga cgctaccgca ggcttaccgc tacgtactgc tgcctaatgc ttatcgcgtt
     1561 atcgtcccgc cgatgacctc cgagatgatg aacctggtga aaaactcggc catcgcctcg
     1621 actatcggtc tggtggatat ggcggcgcag gcgggtaaat tgctcgatta ctcggcccac
     1681 gcctgggagt cgtttaccgc catcacgctg gcgtatgtat tgattaacgc ttttatcatg
     1741 ctggtgatga cgctggttga acgtaaagtt cgcctgcccg gcaacatggg gggcaaataa
     1801 tgtacgagtt tgactggagt tccattgttc cttccctgcc atatctgctc gacgggctgg
     1861 tgatcacgct gaaaatcacc gtcacggcag tggtgattgg tattttgtgg ggcaccatgc
     1921 tggcggtgat gcgcttgtcc agctttgcgc ccgtagcgtg gtttgccaaa gcctacgtta
     1981 acgtattccg ctcgnnnnnn nnnntttcga tgtttgaagc ggcctactat tcagagatta
     2041 tccgtgctgg tattcaaagt atttctcgtg gtcagtcgag cgccgcgctg gcgttgggaa
     2101 tgactcactg gcagtcgatg aaactgatta ttctgccgca ggcgttccgc gcgatggtgc
     2161 cgctgttgct cactcagggc atcgtactgt ttcaggatac ctcactggtg tatgtgttat
     2221 ctctggccga tttcttccgt accgcctcaa ccattggtga gcgtgatggt acgcaggttg
     2281 agatgatcct gtttgccgga tttgtttatt tcgttattag ccttagcgcg tcgttgttgg
     2341 tcagctactt gaaaagaagg acagcataat gattaccctg aaaaatgttt caaaatggta
     2401 tggtcacttt caggtgctga ccgactgctc aaccgaagtg aaaaaaggcg aagtggtggt
     2461 ggtttgcggc ccgtctggtt caggcaaatc aacgctgatt aaaaccgtca acggcctcga
     2521 accggtgcag caaggtgaaa tcaccgtcga tggcatcgtg gttaacgaca agaaaaccga
     2581 tctggcgaaa ctgcgttccc gcgtcgggat ggtgtttcag catttcgagc tgttccccca
     2641 tctgtcgatt atcgaaaacc tgaccctggc gcaggtgaaa gtgcttaaac gcaataaagc
     2701 gccggctcgt gaaaaagcat tgaaattgct ggagcgcgtc gggctttccg cacatgccaa
     2761 taagtttccg gcgcagcttt ccggcggcca gcagcagcgt gtggcgatcg ctcgcgcatt
     2821 gtgcatggat cctattgcca tgctgttcga cgaaccaacg tcggcgctgg atccggagat
     2881 gatcaacgaa gtgctggacg tgatggtcga actggcgaac gaaggaatga cgatgatggt
     2941 ggtgacccac gaaatgggct ttgcccgtaa agtggcgaat cgggtgatct ttatggacga
     3001 gggtaaaatt gtcgaagact cgccgaaaga cgctttcttc gatgatccga aatcggaccg
     3061 cgcaaaagac ttcctcgcaa aaatcctgca ttaatcactc tggcgcgcat actcaattgc
     3121 gcgccatctc acgctttctc gtttcgtccc tcgtatctgt tacatcccgg cgttaccctt
     3181 gtcgcaaaga aacacacaac aaggagcaac aatggcactg ccaattctgt tagattgcga
     3241 cccaggtcat gacgacgcta tcgcaatcgt tctcgccctc gcctcaccag agcttgatgt
     3301 caaagcaatt acgtcttccg ccggaaacca gacaccagaa aaaaccttac gcaatgttct
     3361 gcgtatgctg accttgctta atcgcaccga tattccggta gcaagcggtg cggtaaaacc
     3421 gttaatgcgt aatttgatta ttgcggacaa cgtgcacggc gaaagcggtc tcgacggccc
     3481 ggcattaccg gaaccgacgt tcgcaccgca aaactgtacg gcggtggagc tgatggcgaa
     3541 aacgctgcgt gaaagtgagg aacctgtcac cattgtttct accggaccgc aaactaacgt
     3601 tgccttgctg ctcaatagcc acccggaact gcatagcaaa attgcccgta tcgtgattat
     3661 gggcggcgca atggggcttg gtaactggac gcctgcggct gaatttaata tttacgttga
     3721 cccggaagcg gcagaaattg tcttccagtc aggaattccg gtggtgatgg ccggtctgga
     3781 tgttactcac aaagcacaaa tccacgttga agacactgag cgtttccgcg cgattggcaa
     3841 ccctgtttca accattgttg ccgaactgct ggatttcttc ctcgaatatc ataaagacga
     3901 aaaatggggc tttgtcggcg caccactgca tgacccatgc accatcgcct ggctgttgaa
     3961 accggagttg tttaccactg ttgagcgctg ggttggcgtg gaaacacaag ggaaatatac
     4021 ccagggtatg acggttgttg attattatta tctgactggc aataaaccga atgccaccgt
     4081 aatggtcgat gttgatcgtc agggctttgt tgatttactg gctgatcgtc tgaaatttta
     4141 cgcttaatca ctcgccggat gacatcacgc catccggcgc cactcatttc attacggctc
     4201 gaacgggcgt ctctggaact gatcatgacc acatttcggg catagcgtca gcacttccgg
     4261 tgtatagatc gggagatgga agtgacattt ctcgcagacc agatttccca gccctaccac
     4321 ttctccgctg tgataaaccc catgatgatt gagnnnnnnn nnngaaaact tcgcgccatt
     4381 caagctgcgt tttatcggtg atgtctgcca gctcctgcca caagctttct ttaatcaccc
     4441 gcataaagac gctgtcagac tcttctttca ggctctcttc atagctcatg gcgaactctt
     4501 ccaggtcacg tctgacagct cgcgtcagct catcgacctc ggttcgcgtt aactcccctg
     4561 tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc
     4621 gcaggcgttc gctcaatgac gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg
     4681 tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc cttgctttgc
     4741 taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca tttcaacgtc
     4801 gagtgcgcga aaggctgttt tgacgcgggc gtttgggcta tgctatgcgg atctgaaaaa
     4861 ccacatcaac gctacatttg tagccgtatt gaaaacagga ccactggctg ccatgcaaga
     4921 gcaataccgc ccggaagaga tagaatccaa agtacagctt cactgggatg agaagcgcac
     4981 atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta tgcttcccta
     5041 tccttctggt cgactacaca tgggccacgt tcgcaactac accatcggtg acgtgatcgc
     5101 ccgctaccag cgtatgctgg gcaaaaacgt cctgcagcca atcggctggg acgcatttgg
     5161 tctgcctgcg gaaggcgcgg
//
LOCUS       DAWDRC010000263         5177 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7073_length_5177_cov_2.088442, whole genome shotgun sequence.
ACCESSION   DAWDRC010000263 DAWDRC010000000
VERSION     DAWDRC010000263.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7073_length_5177_cov_2.088442"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    758..767
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1326 a         1335 c         1243 g         1263 t
ORIGIN      
        1 ggcgaacatt atggattacg tagtgcaatg ctggttgtac tggcgctggt tattctcgcg
       61 gctattgtcg cgaaagccgt cgccaaaccc gataccaaaa cgcagacggc gatggagaat
      121 agttgatgag cattaaatta attgcggtag acatggatgg tactttctta agcgatcaaa
      181 aaacctataa ccgtgagcgg tttatggctc agtatcagca aatgaaagca caaggcattc
      241 gctttgtggt cgccagcggg aatcaatatt atcagttgat ctctttcttc cctgaaattg
      301 ctaatgaaat agcctttgtg gctgaaaacg gcggctgggt agtgagcgaa ggcaaagatg
      361 tttttaatgg cgagctgtcg aaggatgcgt ttgctactgt cgtggaacat ttgctgacgc
      421 gcccggaagt ggaaattatt gcctgcggaa aaaatagcgc ctataccctc aaaaaatatg
      481 acgatgccat gaaaacggtg gcggaaatgt attatcaccg tctggaatac gtcgataact
      541 ctgacgaact gatcccacaa gtgcaaaaag cattacatga ggccatcggc gatatcatgg
      601 taccggtcca taccggcaac ggcagcatcg atctgattat ccccggtgta cataaagcca
      661 atggccttcg ccaactgcag aaattatggg gaatagacga cagcgaagtg gtggtctttg
      721 gcgatggcgg taacgatatt gagatgctgc gtcaggcnnn nnnnnnnaat aaccgtgaag
      781 gcgtactgga tgtgatcgat aaagttctta aacacgaagc gccatttaag caataaaaaa
      841 ggcagcacgc ccggcaacac aatacctgaa tgaaggctgc ataattcgcg gaaattaacc
      901 cgctgcatta tttcaggcga aatgattatg aaaaattgtc tactgttggg cgcgctttta
      961 atgggcttta ctggtgtggc gatggcgcaa agtgtcaccg tcgatgtgcc gagtggatac
     1021 aaagtggttg tggttcctga ttcagtcagc gttccacaag cggtcagcgt cgcgactgtg
     1081 ccgcaaaccg tctatgtggc tcctgcgcca gctccggctt accgcccgca tccttatgtt
     1141 cgccatcttg ccagcgtagg tgaagggatg gttatcgaac accagattga cgaccatcac
     1201 cattaattcg agaatgcctg atcgcacaaa gcaatcaggc atttttttac ccttcgtgag
     1261 aatttcccat ctgtttatct tttaaaaaga taaccatcag cgacagccac aaaatgccgt
     1321 tgacaaggtt gaagagatta aacagtccgt tgcccccgtt cagccaggca tgtttgctga
     1381 tttcaatacc gacggtaaag atcagcattt gcagcatccc catcgccgca gaaaccgtac
     1441 ctttactcat atcgctggca aacagggtta atcgcaccag tcccgcattc gccagaccaa
     1501 taccgaaagc ataaatactt aacccggcgg tcatccataa atacgcgtgc gatgagataa
     1561 ccgttgccgc agcagcgacc aacagaccaa tcataatcgg ccagccaccc ataataatca
     1621 gcgaacgtac ggtgcggcgc gaggtcagac gcgctaacag caagttaccc gcaattaacg
     1681 ccccgaaaat aggcacttgc agcaagccat attcatagct gctcaactgc tcaccggtaa
     1741 tgatgataat cggcgactgg gcgatccacg ccagcaacgg caggctaacg aatcccagcg
     1801 ccagcgcccc cgccacaaag cggccgttct tcagcaccag cttatagtca cgaccgagtt
     1861 ctttcagcga cagtttctcg cctatacgcg tggcggtttc aggcatagct cgttgcagac
     1921 cgaaaaagga gatcgctgcc aatgcggcaa acaagacaaa catcccttcc cagggcagca
     1981 catggatcca cgccgcgccc accagcggac caagtagtgg agcaatcagc gccacgttcg
     2041 ccattagcgc ggtgatcttg atacaaacgg cctcttcgaa ggattcctga attgcggcgt
     2101 atcccacagc accaatgaaa cagaggctta tgccctgcaa gaagcgcaac agggtgaatt
     2161 gttcaatgtt ttgcgccagt aatattgcca gacaggtgac gataaaccac accactcccg
     2221 ccaacatcac cggacggcgg ccaatacgat ccgacaacgg ccccaggagc cattgtaaaa
     2281 acatcccgcc cgccagatac gcagtcatcg aagtaggaac ccaatcaatg cccgcctgat
     2341 attgttccac cacggccaac ataccgggtt gaatcatatc gttgccgata taggttgaaa
     2401 attcgtaaag caccagacag agagggaaaa gtaacgcctg acgtccaagc ctggcaccgg
     2461 aagctaattt attttgcatg caatttcttc gccaataata atcgcgcaaa gtttaataaa
     2521 agcgcagcta acgagaaagc gaattttgta gctgaaacca cggttaagca cattcttaca
     2581 ttattgcgag tatagctacg ctttctttaa gttttattta acctttgccc gttacaatca
     2641 cctaccgtaa acaggccgct tgagggaaat aagacgatgc cgctttaccc agtttaacct
     2701 gcactttatt ctcaacgact tgcctttatt ggctcccttt taatcacttt gcgtcgggaa
     2761 gttatgctgg aaaatttgaa tctctctcta ttctctctta ttaacgcaac gccagactct
     2821 gcgccgtgga tgatctcgtt ggcgattttt attgctaaag atttgattac cgtggtgccg
     2881 ttgctggccg cagtactttg gttgtggggg cttacagcac aacggcaact ggtgataaaa
     2941 atcgctatcg cgctggcggt cagcctgttt gtgtcctgga cgatgggaca tctttttccg
     3001 cacgaccgac cctttgtcga aaatatcggc tataacttcc tgcatcatgc ggcggatgac
     3061 tcattcccga gcgatcacgg tacggtgatt ttcacctttg cactggcatt tttatgctgg
     3121 catcgcctgt ggtccggctc acttttaatg gtgctggccg tcgtcattgc ctggtcgcgc
     3181 gtttatcttg gcgtccactg gccgctggat atgctcggtg gattgctggc aggtatgatt
     3241 ggctgcctta gcgcccagat tatctggcaa gcgatggggc ataaactcta tcaacgtctg
     3301 caatcgtggt atcgcttctg ttttgcatta ccgatccgca aaggctgggt gcgtgactga
     3361 tctctgctaa aaagtgtagt attgagcggc tcgcttcaat aactattcag agggattatg
     3421 gaaacacgtc gcgaagagcg tatcgggcag ctgctgcaag aattaaaacg cagtgataag
     3481 ttacatctta aagacgccgc cgccctgctt ggggtttcgg agatgacgat tcgtcgcgat
     3541 ctgaacaacc acagtgcgcc cgtcgttttg ctcggcggct atattgttct ggaaccacgc
     3601 agcgccagcc attacctgtt aagcgatcaa aaatcccgcc tggtggaaga aaaacgccgg
     3661 gcggcaaaac tggctgcgac gctggtagaa cccgatcaga ccctcttttt tgactgtggc
     3721 accaccacgc cgtggattat tgaagcaatt gataatgaaa tcccttttac cgccgtttgt
     3781 tattcgctaa atacctttct ggcgctgaaa gagaaacccc attgccgcgc gtttctttgc
     3841 ggtggtgaat ttcacgccag caacgccatt ttcaaaccca tcgattttca gcaaacgctg
     3901 aataattttt gcccggatat cgctttttat tccgctgcgg gcgtgcatgt cagtaaaggc
     3961 gctacctgct ttaatcttga agagttgccg gtaaaacact gggccatgtc gatggcgcaa
     4021 aagcatgtgc tggttgtcga ccacagtaaa tttggcaagg tgcgtccggc gcgcatgggt
     4081 gacctgaaac gctttgatat tgttgtgagc gattgttgcc cggaagatga gtatgtgaag
     4141 tacgcgcaga cgcagcgtat taagttgatg tattaatgac gtataaccgg atgacgtttc
     4201 gcgccatccg gttatcagaa gattaagaga accagctgcc gaaccactga tggaatttca
     4261 tcatcacgaa atcccacatc cgaccaaaga atccgccctc ttccacattt tccatcacga
     4321 tcagcggacg ctgctcaatg gatttaccgt taagctggaa atcaatggtg ccgacaacct
     4381 gacctttttt cagcggtgcg gtaagctgcg gttccgttaa cgtataactc gccttcaggt
     4441 ttttcagctg cccacgcggt atggtcactg agcccgcttc gcctgcccga gattcacttc
     4501 gctcttatca ccaaaccaga cgcgctgagt cacaaaggtg gcatcaggtt taattggcgt
     4561 cacggtttca aagaagcgga aaccccaggt caataatttc tcagactcat taaaacggat
     4621 acggtcagtt ttcgccccca gcactacgga gattaaacgc atatcgccct gggtagccga
     4681 agcaaccaga ttatatcccg cgcctgccgt ggttcctgtc ttcatgccat caacattcag
     4741 attgctgctc cacagcagac gattacggtt aggctgacga attttgttga aggtgaactc
     4801 tttctcttta tgaatggcgt actcttccgg cacatcgtgg atcaatgctt tacccagcaa
     4861 tgccatatcg cgcgcggtac tgaactgccc cggcgcatcc aggccgtgca ccgtctggaa
     4921 ggtagtgttg gtcagaccca gttttttggc ataaccattc atcaaaccaa taaatgactc
     4981 ctggctcccg gcaacgtaat cagccagcgc aatacaggcg tcattaccgg actggataat
     5041 cacacctttg ttcaagtctg ccaccgaaac ctgatcgccc ggtttgagga acattaccga
     5101 tgaaccacgc agtgccggat ttcccgtcgc ccaggcatct ttaccgaccg tcaccatatc
     5161 ggtgagttta atcttat
//
LOCUS       DAWDRC010000264         5170 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7086_length_5170_cov_2.046334, whole genome shotgun sequence.
ACCESSION   DAWDRC010000264 DAWDRC010000000
VERSION     DAWDRC010000264.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7086_length_5170_cov_2.046334"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1363..1372
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1819..1828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4377..4386
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1269 a         1299 c         1295 g         1277 t
ORIGIN      
        1 gcaacaataa tatatttaaa aagattatat tattcaactt tatggtgagg attacataat
       61 gagcagtcca aatattctct taacccgtat tgataaccgt ctggttcacg gtcaggttgg
      121 cgtgacctgg acatccacca tcggtgcgaa tctgttggta gtcgtggatg atgttgtcgc
      181 taacgatgat attcaacaga aattaatggg tattaccgca gaaacctacg gctttggcat
      241 tcgtttcttt actatcgaaa aaaccattaa cgtcatcggc aaagctgcac cacatcagaa
      301 gatcttcctg atttgccgta cgccacaaac ggtacgtaaa ttggtagaag gtggtattga
      361 cctgaaagat gtcaacgtcg gcaatatgca tttctcggaa gggaaaaagc aaatcagcag
      421 taaagtttat gtcgatgacc aggatctcac ggacttacgt tttattaaac aacgtggcgt
      481 gaatgttttc attcaggacg tccctggcga tcaaaaagaa caaatccctg actaaatcta
      541 aaatcgcctt aatattggtt tgaggtaata aaaatgcatg aaataaccct actccaggga
      601 ttatccctgg cggcgttagt tttctttctg gggattgatt tttggctgga agccttattt
      661 ttattccgcc cgataatcgt ttgtaccctt acaggtgcta ttctcggtga tattcagact
      721 ggcttaatta ccggtggcct gacagagttg gctttcgccg gattaacccc tgcaggtggt
      781 gttcagccgc ccaaccctat tatggcgggt ctgatgacca ctgtcattgc atggtctacg
      841 ggcgttgatg ccaaaacggc aattggtctt ggcctgccgt ttagtttgtt aatgcagtac
      901 gtcattctgt tcttctattc cgctttctca ttatttatga ccaaagccga taaatgcgca
      961 aaagaggcgg atacggcagc attttcccga cttaactgga caacgatgct catcgtcgct
     1021 tcagcgtatg cggtgattgc tttcctctgt acttacctgg cacaaggggc gatgcaggcg
     1081 ctggtgaaag cgatgcccgc ctggctgacc cacggctttg aagtggctgg cggtattctg
     1141 cctgccgttg gttttggctt gctgctgcgc gtgatgttca aagcgcaata tatcccttac
     1201 ctgatcgccg gtttcctgtt cgtttgctac atccaggtca gcaacctgtt gccggttgcc
     1261 gtactgggcg caggctttgc ggtgtatgag tttttcaatg cgaaatcccg gcagcaagcg
     1321 caaccgcagc ccgttgccag taaaaatgaa gaagaggact acnnnnnnnn nnaagtgaaa
     1381 acgggaacgc ttacgatcac aaagaaaatc agtaaaaaag atatcacccg tctgggcttt
     1441 cgttcgtcac tgctacaagc aagctttaac tacgaaagga tgcaggcggg cggttttacc
     1501 tgggcgatgt tgccgatcct gaaaaagatt tataaggacg acaaaccggg cctaagcgcg
     1561 gcgatgaaag ataacctcga atttattaat acccacccta atctggtcgg attcctgatg
     1621 gggttattaa tttcgatgga agaaaaagga gaaaaccgcg acaccattaa aggcctcaaa
     1681 gtggcgctgt ttggcccaat cgccgggatt ggcgatgcga ttttctggtt taccttgttg
     1741 ccaattatgg cggggatttg ctcatcgttt gccagtcagg ggaacctgtt gggaccgatt
     1801 ttgtttttcg ccgtttacnn nnnnnnnntt cctgcgcgtc ggctggaccc acgtcggtta
     1861 ttcagtcggc gtgaaggcta tcgataaagt gcgagagaac tcgcagatga ttgctcgttc
     1921 ggcaaccatc ctcgggatca cagtaatcgg cgggctgatc gcttcgtatg tgcatattaa
     1981 cgtggtgacg tcgtttgcca tcgacagtac ccacagcgtc gcactgcaac aggatttctt
     2041 cgataaagtc ttcccgaata ttttaccgat ggcctacacc ctgctgatgt attacttcct
     2101 gcgggtgaaa aaagcgcatc cggtgctgtt aatcggcgtg acgtttgttc tttctattgt
     2161 ttgttctgca ttcggcattt tgtaaatgga acgaggcacc gcgtctggcg gtgcctcttt
     2221 acttaaggaa tttcatcctg tgcaacccct tcaacaagtt gaaaactata cggcattaag
     2281 tgaacgtgcc agcgaatatt tattggccgt gatccgtcgc aaaccggatg ccgtgatttg
     2341 cctggcgacc ggagccacgc cattactgac gtatcattat ctggtagaaa aaatccacca
     2401 gcagcaggtt gatgtcagcc atctcacctt cgtgaagctc gacgaatggg tggatctgcc
     2461 attaacgatg cctggcacct gcgaaacttt cctacaacag catatcgtgc agccgctggg
     2521 gctgcgtgaa gaccagctca tcagctttcg ctccgaagag ataaatgaga cagagtgcga
     2581 acgggtaacg aacctgattg cgcgcaaagg cggtctggat ttatgcgttc tcggattggg
     2641 gaaaaacggt catcttgggc tgaacgaacc gggagaaagc ctgcaaccag cctgccatat
     2701 cagtcaactt gatgccagaa cacagcaaca tgagatgtta aaaaccgcgg accgtcccgt
     2761 gactcgtggg atcaccttag gcctgaagga tattctcaat gcccgcgaag ttttgttact
     2821 ggtgactggc gaaggaaaac aggatgcgac agaacgtttt ctcacggcta aagtctccac
     2881 cgctatcccg gcttcatttt tatggctgca taaccatttc acatgcgtga ttgatgaaat
     2941 gtgtcgtcgt taactcgcca ggtaaaggcg ttgtagcgct attcctgctg cgacgcctgt
     3001 aatcactctc cctgctgctc cagcgcatac ttatacagcg catttttctt cacgccgtga
     3061 atttctgcgg ccagcgccgc cgcttttttc agcggcagtt ctgcctgtag cagcgccagc
     3121 gtgcgcaggg catcagcagg caagtcattt tcctgcgctt tatggccttc gacaatcagc
     3181 accatttcgc ctttgcgacg gttttcatct tcttttaccc acgccagcag ctcgccaacg
     3241 ggcgcgccgt gaatggtttc ccaggttttg gttagctcac gcgccagaac cacgtaacgg
     3301 gattcgccta ataccgcaac gatatcttcc aggctatcta acagacggtg ggtagattca
     3361 taaaaaatca gcgtgcgcgg ctccgcttca atagctttta gcgcatcacg gcggcctttt
     3421 gatttggcag gtaaaaagcc ttcataacaa aaacgatcgg atggtaaacc cgcggcgctt
     3481 aacgcagtga tagcagcaca cggccctggt agcggcacca cgcgaatccc cgcttcacga
     3541 caggtacgca ccagatggta gccaggatcg ttaattagcg gcgttccggc atcggaaacc
     3601 agcgcaatgt tttgcccctc ttgcagcttc gccagcagcg tttcggcttt ttgttgttca
     3661 ttatggtcgt gcagcgcaaa caaccgggca ttaatcccaa aatgttgcag caataaaccg
     3721 gtgtgacgcg tatcctcggc ggcaatcaga tcaacggcct gtaatacctc caacgcacgc
     3781 tgggtgatat ccgccagatt gccgattggc gtcggtacaa tgtaaagctg cccctgagaa
     3841 ttatccgccg attggtgttg tttcattgtg tcgtccgtat tgccgattta atattgagca
     3901 ttgcgtaaaa aaaaatatca ctggatacat tatggtaccc tcaacatttt ctcgtttgaa
     3961 agccgcgcgt tgtctgcctg ttgttctggc agccctgatt ttcgccggtt gtggcaccca
     4021 tactcccgat cagtccactg cttatatgca gggcacggcg caggctgatt ctgcctttta
     4081 cctgcaacag atgcagcaaa gttctgatga taccaggatc aactggcaat tactcgccat
     4141 tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt gagttgttta accaactacc
     4201 gcaagaactg aatgatactc agcgtcgcga gaaaacactg ctggcggcag agataaaact
     4261 ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg aaaatcacac ctgccgattt
     4321 agaacaaaac cagcaagccc gttactggca ggcaaaaatc gatgccagcc aggggcnnnn
     4381 nnnnnnatac gctggtgatc aacgccgacg aaaatattct gcaaggctgg ctggatttgc
     4441 agcgcgtctg gtttgataac cgtaacgatc ctgacatgat gaaagctggg atcgccgact
     4501 ggcagaaacg ttatccgaac aacccgggcg cgaaaatgct gccaacgcag ttggttaacg
     4561 taaaagcgtt taaaccagca tcgaccaaca aaatcgccct gctgttgccg ctgaatggcc
     4621 aggcagcggt atttggtcgc actattcagc aaggctttga agcggcgaaa aatatcggca
     4681 ctcagccagt ggcggcccag gtagctgccg cacctgccgc agacgtagca gaacaatctc
     4741 agccgcaaac cgtggatgac gttgccagcc cggcacaagc ctcggttagc gatctgaccg
     4801 gtgaacagcc tgcggcccag ccggtgcctg taagcgcccc ggcgacaagc accgcagcgg
     4861 taagcgcacc cgcaaatcca tccgcagagc tgaaaatcta cgatacctca tcacaaccac
     4921 ttagccagat cttaagccag gttcagcagg atggcgcgag tattgtggtc ggtccgttgc
     4981 tgaaaaataa cgttgaagag ttgctgaaga gcaacactcc gctgaacgtg ctggcactga
     5041 accagccgga gaacatcgaa aaccgcgtca atatttgtta cttcgcgctt tcaccggaag
     5101 acgaagcgcg cgatgcagcg cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc
     5161 tgatcccgcg
//
LOCUS       DAWDRC010000265         5154 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7102_length_5154_cov_2.289272, whole genome shotgun sequence.
ACCESSION   DAWDRC010000265 DAWDRC010000000
VERSION     DAWDRC010000265.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7102_length_5154_cov_2.289272"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4584..4593
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1263 a         1352 c         1283 g         1246 t
ORIGIN      
        1 gtgcttcgct gcttctgcaa gcaactggtt acgcgcctga gtcagttttt cgtgaccaag
       61 gccagcctgg tcaatcagct caaagtcaaa gccggttgcg gtacccagtt ccacgattgc
      121 gggcaggtta aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcac gtgttgcacg
      181 catggtaatc gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga
      241 aacgaacgca ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa
      301 caccgactca acgttgttct tttctttggt cagatagtaa ttcgttacct cattgagcac
      361 tttctgcgta cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc
      421 ctggtcctca tctggcaaga aggagcttgg cagacgcacg aacagatagg ccataccgac
      481 cacgatgatc agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct
      541 gtcggtgtag tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt
      601 tttaccttcc ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg
      661 agtcaggatc aacgccacca gtaccgacag cgccattgct gaaacaatgg taatagagaa
      721 ctgacgatag atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga
      781 cagtaccatc gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtggc
      841 ttcttttggc ggcaaacctt cttccgccat cacacgctca acgttttcta ccacaacgat
      901 ggcgtcatcc accaacaggc cgatggcgag caccatcccg aacattgtta gcgtgtttat
      961 cgagaagcca aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat
     1021 ggtcggaatc aacgtcgcgc ggaagttctg caagaacaga tacattacca ggaacacgag
     1081 gatgatcgct tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggtgt
     1141 ggtgtcatac gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag
     1201 ttcagcacgg attgccgcag cggtatccag cgcgttcgca ccggtcgcca gcttgatccc
     1261 cagaccggaa gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag
     1321 ctcaattttc gccacatcac gcagcagcac gcgggaacca tcctgattca ctttcagcag
     1381 gattttgccg aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag
     1441 ctgttggcct ttcaccggcg gcgtaccacc gagctgacca gctgcaacct gggcgttctg
     1501 tgctttgatg gcggtaatga catcaaccgg cgttagctgg aatttgttca gttcattcgg
     1561 gttcatccag atacgcatcg cgtactgtga accgaacaac tgaacgtcac ccacgcccga
     1621 cgtacggctg atggcatctt tcatattcgc cgccacgtag tcggagatat cctcctgcgt
     1681 catggtgcca tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc
     1741 aacgctcacc ccttgctgct gtacttcttg tggcagcaac ggcatcgcca actgcagttt
     1801 gttctgtacc tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat
     1861 ctgcacggta cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt
     1921 catattctgt tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccagg
     1981 gtaggaggcg gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag
     2041 tttgaggatc gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat
     2101 cgggcgatcg ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg
     2161 actgttcagg ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg
     2221 cttttacctg gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg
     2281 ctttcagacc ttctgtcacc agccacttat cgccgatagc ctggcttgca acgatcggac
     2341 gggtttccac tttgtcatcc gcgccaacca ccagtacggt ggcatcgcca cgcggcgtac
     2401 gggttacgcc ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg
     2461 cacgcacgaa catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg
     2521 tgatagaccc agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg
     2581 ggaacttaat gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca
     2641 gcgtgccatt cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat
     2701 caacgtagat cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta
     2761 ccaatgcgcc ttccgtcacg tttgacttac caatgcgacc actaatcgga gaggtaactt
     2821 tggtgtaagc cagattgatt cgcgcagttt caacggcagc tttcgccgca gttaccgcag
     2881 cattcgcctg ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag
     2941 taccgagcaa tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg
     3001 ctttcgccag atcacctttc gcactgtcgt atgccgcctg ataggtcgca ggatcaatct
     3061 gatagagaga gacacctgct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa
     3121 tcccgctaac ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct
     3181 cggttgtgat ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct
     3241 ggccaccttg ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca
     3301 tcagaacgac cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt
     3361 ccgatttcaa attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc
     3421 gtcgtgctat ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga
     3481 acatatggca cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt
     3541 ggctctacgt cttttctcac agcagggggt atcatccacc tcgctgggtg agattgcaaa
     3601 agcagctggc gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt
     3661 cagtgagatc tgggaactgt cagaatccaa tattggtgaa ctcgagcttg agtatcaggc
     3721 aaaattccct ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgagtc
     3781 cacggtgaca gaagaacggc gtcgattgtt gatggagatt atattccaca aatgcgaatt
     3841 tgtcggagaa atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga
     3901 ccgtatagaa caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat
     3961 gacgcgtcgc gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct
     4021 ctttgccccg caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact
     4081 ggagatgtat ctcctgtgcc ccacgcttcg taaccctgcc actaacgaat aaccctgaat
     4141 ctgactccag gaattttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg
     4201 atattcttgc ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca
     4261 tgactatgtt ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt
     4321 ttgtttttgt cttgttatgc cagaacacgg cgtttgcacg ggcgtcatcg aatggtgatc
     4381 tgccgacaaa agcggacctg caggcacaac ttgactcact gaataaacaa aaagatcttt
     4441 ctgctcagga caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa
     4501 tcgatcgcgt aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa
     4561 aaatgcgcca ggcgaccgcg gcgnnnnnnn nnntttgaca ggcgctggcc ggtgtgcaga
     4621 acgtatgaaa cgttcaggcg acatataata gccaactggt ttcgctacag acgcagcccg
     4681 aacgcgtaca aaatgcgatg tataacgctt cgcagcagct gcaacaaatt cgcagtcgtc
     4741 tggatgggac tgatgtcggc gagactgcct tacgtcccag ccagaaagcg ttaatgcagg
     4801 tccagcaggc tttgctgaat gccgagattg accagcagcg taaaagcctg gaagggaaca
     4861 ctgtcttgca ggataccttg caaaagcagc gtgattacgt gacggcgaac agcgcccgtc
     4921 tggagcacca gttacaactg ttgcaagaag cggtaaacag caagcgcctg actttaaccg
     4981 agaaaacggc gcaggaagct gtctccccgg atgaagccgc gcgtattcag gctaatccgc
     5041 tggtgaagca ggaactggaa attaaccagc agttaagtca gcgtctgatt accgcgactg
     5101 aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa aaactggctg gagc
//
LOCUS       DAWDRC010000266         5139 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7122_length_5139_cov_1.922109, whole genome shotgun sequence.
ACCESSION   DAWDRC010000266 DAWDRC010000000
VERSION     DAWDRC010000266.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7122_length_5139_cov_1.922109"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1179..1188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1936..1945
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3784..3793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4568..4577
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1320 a         1255 c         1373 g         1151 t
ORIGIN      
        1 ttataaatgc actttgcgtg ccgcccgtga ctacgcggca cgccattttc gaataacaaa
       61 tacagagtta caggctggaa gctatgtcaa acaaaccctt tatctaccag gcacctttcc
      121 cgatggggaa agacaatacc gaatactatc tactcacttc cgattacgtt agcgttgccg
      181 acttcgacgg cgaaaccatc ctgaaagtgg aaccagaagc cctgaccctg ctggcgcagc
      241 aagcctttca cgacgcttct ttcatgctcc gcccggcaca ccagaaacag gttgcggcta
      301 ttcttcacga tccagaagcc agcgaaaacg acaagtacgt ggcgctgcaa ttcttaagaa
      361 actccgaaat cgccgccaaa ggcgtgctgc cgacctgcca ggataccggc accgcgatta
      421 ttgttggtaa aaaagggcag cgtgtatgga ccggtggcgg tgatgaagca gcgctggcgc
      481 gcggtgttta taacacttat atcgaggata atctgcgcta ctcgcaaaac gcgccgctgg
      541 atatgtataa agaggtgaat accggcacca acttgccagc gcagatcgat ctttatgccg
      601 ttgatggcga cgagtacaaa ttcctctgta tcgccaaagg tggcggttcg gcaaacaaga
      661 cgtatctcta tcaggaaacc aaagcgttac tgacgccggg aaaactgaaa aattacctgg
      721 ttgagaagat gcgcacgctg ggtacggcgg cctgtcctcc atatcatatt gcgttcgtta
      781 ttggtggaac ttctgcagaa acgaatctta aaacggtgaa actcgcttcc gctaaatact
      841 atgatgaact gccaacggaa gggaatgagc acggtcaggc gttccgcgat gtggaactgg
      901 aaaaagaatt gctgatcgaa gcgcaaaatc ttggtctggg tgcgcagttt ggtggtaaat
      961 acttcgctca tgacatccgc gtgattcgcc tgccacgtca cggcgcatcc tgcccggtcg
     1021 gtatgggcgt ctcctgttct gctgaccgta atatcaaagc gaagatcaac cgtcagggga
     1081 tttggatcga aaaactggaa cataatccag gcaaatatat cccggaagag ctgcgcaaag
     1141 caggagaagg cgaagcggtg cgcgttgacc tcaaccgtnn nnnnnnnntc ggtcgtgata
     1201 ttgctcacgc caaactgaaa gagcggatgg ataacggtga agggctgccg cagtacatta
     1261 aagatcatcc gatttactac gcgggtccgg ccaaaacgcc ggaaggttat gcctccggtt
     1321 ctcttggccc aacgaccgcc ggacggatgg attcttatgt cgatcaactg caagcgcagg
     1381 gcggaagtat gatcatgctg gcgaaaggca accgcagcca gcaggtgacg gatgcctgta
     1441 aaaaacacgg cggcttctac ctcggcagta tcggtggtcc ggcagctgta ttggcgcagg
     1501 gaagcattaa gagcctggaa tgtgttgaat atccggaact gggaatggaa gccatctgga
     1561 aaattgaagt ggaagatttc ccggcgttta tccttgtgga tgataaagga aatgacttct
     1621 tccagcagat acaactcaca caatgcaccc gctgtgtgaa ataaacagag ccgcccttcg
     1681 gggcggtttt tttacatggc acgaaagacc aaacgtttgt tatcaaatgg taaataatga
     1741 gtgagctaaa agttgcttaa cgaaagcaaa acagaaagaa aaaattaatc aggtgaggag
     1801 caggccatga atacagtacg cagcgaaaaa gattcgatgg gagcgattga tgtcccggca
     1861 gataagctgt ggggcgcaca aactcagcgc tcgctggagc atttccgcat ttcgacggag
     1921 aaaatgccca cctaannnnn nnnnnctgaa gagaaagcga gcgccattcg gcaggcggcg
     1981 gatgaagtac tggcaggaca gcatgacgac gaattcccgc tggctatctg gcagaccggc
     2041 tccggcacgc aaagtaacat gaacatgaac gaagtgttgg ctaaccgggc cagtgaatta
     2101 ctcggcggcg tgcgcgggat ggaacgtaaa gttcacccta acgacgacgt gaacaaaagc
     2161 caaagttcca acgatgtctt tccgacggcg atgcacgttg cggcactact ggcgctgcgc
     2221 aagcaactca ttccacaact taaaaccctg acccagacgc tgagtgaaaa atcccgcgca
     2281 tttgctgata tcgtcaaaat cggtcgtacc cacttgcagg acgcgacgcc gttaacgctg
     2341 gggcaggaga tttccggctg ggtagcgatg ctggagcata atctcaaaca tatcgaatac
     2401 agcctgcctc acgtagcgga actggctctg ggcggtacag cggtgggtac tggactaaat
     2461 acccatccgg aatatgcgcg tcgcgtagca gatgaactgg cagtcattac ctgtgcaccg
     2521 tttgttaccg cgccgaacaa atttgaagcg ctggcgacct gtgatgccct ggttcaggcg
     2581 cacggcgcat tgaaagggtt ggctgcgtca ctgatgaaaa ttgccaatga tgtccgctgg
     2641 ctggcctctg gcccgcgctg cggaattggt gaaatctcaa tcccggaaaa tgagccgggt
     2701 agctcaatca tgccggggaa agtgaacccg acgcagtgtg aagcattaac catgctctgc
     2761 tgtcaggtga tggggaacga tgtggcgatc aacatgggtg gcgcttccgg taactttgaa
     2821 ctgaacgtct tccgtccgat ggtgatccat aatttcctgc aatcggtgcg cttgctggca
     2881 gatggcatgg aaagtttcaa caaacactgc gcagtgggca ttgaaccgaa tcgtgagcga
     2941 atcaatcaat tactcaatga atcgctgatg ctggtgactg cgcttaacac ccacattggt
     3001 tatgacaaag ccgccgagat cgccaaaaaa gcgcataaag aagggctgac cttaaaagcg
     3061 gcggcccttg cgctggggta tcttagcgaa gccgagtttg accgctgggt acggccagaa
     3121 cagatggtcg gcagtatgaa agccgggggt taatctgcaa catacaggtg cagccgtgga
     3181 atgattaaac gaagcggctg cgcctgtggt ttataacggt gctgcacatt gtcggcatcg
     3241 taatttaaca actcaccaac gtccggcacg cccgcgccat tatccctatt aatcagtgca
     3301 atcagcggcg tagggcaggc gtgttgtact tgtttttgat ctcctttgta ccagacgcgg
     3361 gcaatcggct gcaccttcac cggacgtttg atttttaact tcgcgttctg tggcagggca
     3421 gcgatatcct gatattctcg ctccagtttt ctttgccact cctcgcgcgt ccacggtgcg
     3481 acactgcgtg gtgatttcag gcttttttcc agctgtgcca ggacttcatc acgatgcaaa
     3541 tttttaatga tatgtttatt agcccagcca aaacgtaaag tggcggggtc gtgcagaacg
     3601 gtgagcgtgc ggtaagcatt aagtgtaatc agccccggca aatgacgatg cacccattca
     3661 aaacgtgccg cggaggggag ttctgattca accgtgacga tatgctcgaa cgtggtcttg
     3721 agtttattga tgtgctgaat atgactgact aacgctgctt gcgaaaagtt atcgacctgg
     3781 taannnnnnn nnncggcctt gctgctgcga ttttcggact gttgttgaat aaataaattg
     3841 cgaaaatgac gcaacgccag cgattgcgcg tcgttgccga gatgttgttt tacctcaata
     3901 cgattaagcg gattatgctc atcctctttt tttacctccg gcaaagagaa cacgcgggca
     3961 accaatagct tgtgttgctc aagatgagcg gcaaatgcag ccagctcttg ttccatctgg
     4021 cgaaaagtag tgttgagtcg gtctacgaga tcgtaacgcg ccataatatt aaccacttta
     4081 gttacaacat ccttatttta tagcacaatg gtggtgactt gaccaccgtg cgcatagtgt
     4141 caggcagagg taaattgcgg gatgttatgc cataacggcc agctgaacga gaagcgggca
     4201 ccacccagtt cgctggtgtc acaattaacc gtaccgccca ttgccagtgc tatagagtgg
     4261 acaatggcca gccccagccc gcagccgccg gttgagcgat cccggctggg atcgaggcga
     4321 acaaaaggtt caaagatatg ttcgcggttt tctggggcaa tccctgggcc atcatcctca
     4381 acaattaatg tcgctctatt ccccgacagt agcaggctgg tttcaaccgt tgagtgacag
     4441 tagcgcaggg cgttattgag caaattatcc agcacgcgct ccattaagcg catatccaac
     4501 gccgcataat ggccttgcgc gagcgttttt atccgtaccg ttttatcggg cgttactgcc
     4561 tgaatatnnn nnnnnnntga atgtctgcca gatgcgttga cagccacaac ggcaggtctg
     4621 gttcgctaag atgaagctcg ttttgtgggc gatcgagtcg ggcataagtc agaagctctt
     4681 caattaaagc ttcaagttga ctgatatcgc ggttcaacgc ctgggattcg gcggcgctca
     4741 ggttatcgct catctccagt cgataacgca ggcgcactaa cggtgttcgc agttcgtgag
     4801 cgataccgtc aataagctgt tttttgctgg caatcaaggc gttgatattg tccgccatct
     4861 ggttaaatgc aacaccaagt cgttcaaagc tcgaaccctc atcaaagtgg atacgttcat
     4921 tgagatgccc atcgccaaat cgttgcgccg ctgcttccag ttttaacata tcctgccagt
     4981 gcggacgcat ccagataaac accggaaagg cgagggaaat ggcaataaaa gcgatcaggg
     5041 cgatatccag taatcgcatc tgatggaggt aataaagata aggaacagga ccaactgcca
     5101 gcacgtagtg gctgcgcggg atacgttgca aaaacgtgt
//
LOCUS       DAWDRC010000267         5113 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7165_length_5113_cov_2.517200, whole genome shotgun sequence.
ACCESSION   DAWDRC010000267 DAWDRC010000000
VERSION     DAWDRC010000267.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7165_length_5113_cov_2.517200"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1426 a         1148 c         1094 g         1445 t
ORIGIN      
        1 gaagacatcg tggctgagaa caagctgatc ggcgtatccc aactcaatga gatgagcgac
       61 ggcatcggca gtctcctgca cccctggcgc tattccctct ttcgggaagg taatgtctag
      121 gccaatcatg tcgaattcca gccagacacc cttatcaagc attttccgct gatacgccac
      181 gtcttttcct gacggatccg agtgtgcgag agagacttta tttggcgaga cgcccatttc
      241 acctaacaca atgtcgagta cttcatcacc gcgacgaagc cagcccggca tgtgaatatt
      301 catcgccaca tgaggattgt taatctgtgc cagcgaggca gcccgcaagc tgttatgctc
      361 ggcttcggta aatgtcggtg agacacctat ttcaccgatc attccggcac gaatatccgt
      421 atcgccaatc ccttgattca attctttatc gatcgttgcc gccagttcat caaccgtttt
      481 atgaattctc tggctttcaa atttttccag gtagggaccg gaggaagcaa caatattcag
      541 acctgttttt aatgcgactt ctcgtaatgc ctgcgcatct cgtccgatag actcagaccc
      601 tgtggcatca acaatggtgc gaccaccgag cgagataaag ttattaattt cgaagatcac
      661 atcttcaata ggtttgcggt ccatattatc agcgcagcaa taaggatcgt gttttaatgc
      721 ccactggatt tctgccgtga cttttttatc aacaagccgt tgtgaaaaag gatagcaagg
      781 agcatccaca acagaggaaa gatcgttgaa cagatgttca tgaggaagtg ttagccccat
      841 atcttcacgg gcgacggggc ccgtcacagt ttgtagataa tctttcatga ttatacctcc
      901 attttcttca ttgttttcga ggttctgtat tccggaatag tggtaacaat tgcaccgacc
      961 agtgcaaaca gtgttccgat gatcgtgacc agataaacgg tattgcccag cgatggaatt
     1021 aatacgtcaa tcaatactga tcccagtaac tggcccgccg ttgaagcaac ccccagcatc
     1081 agtaacccca gacctctgac caaaatggcc attaaaccga tagacattag tccaagcggg
     1141 ccgccaagat acatccacca gacatctggt aattgaacag tgacgtgccc taatgcgaca
     1201 cgaatagcta gcgctgtgcc taaaacacaa aatccaacga tgaaattcca ggtgattgaa
     1261 accagcatgg atcccgtagc ttcagctact tttgcatttc cagcgggctg ccatcctgca
     1321 agcaaaccag ctaagaacgg aagaatggca agtaagatga aagaggtaga atgccactgc
     1381 ggtgacacaa caaaaatcgt agcaataacc gcaaataatg caccgatgat gcgataaggg
     1441 gtaaagtatt ttttctcgtc gacaccgata ccaaagcgat cgcaaagtaa accagacagc
     1501 aatagcgcgg agatcagtgc ggtttgaaac gttgcgaccc cgagtgcgct ggctgatgcg
     1561 ccttcagaaa ataccaccat cgctccacag agaccggcaa accagttcca gagaggaact
     1621 tttcggtttt taatcagaga aggaattgcc gcaaattgtt gccgggtttc ttttcgcgcc
     1681 ataataataa aaaacatcac gactaaacca ctggcaaagg agataactgc gcatgcatta
     1741 ccatcctgta accagtgacc taattgacca ttcaccgctg attgcatagg ggagagcata
     1801 cccgcaagaa tcgtggcaag catccataag ggagttgagt acttattctt tttcatacat
     1861 caatccttct attatagtaa atggggtaac tggagttacc ccattattat tatcttgcaa
     1921 tatgagtcag tatatttacc cacaacgcgg tatagaacgg ccaggacata aactgctgtg
     1981 taccccagtg cggtgaacag tcactcataa agcaggctat tttcccgttg tgatagttac
     2041 caaagaccag taatggatcg ttgtttattg ttaataccac ctcagcattt tctttagcga
     2101 tcgcccggtt ataacctagg aaaaaaggat attcagagaa ccctttaata actggatgtt
     2161 caggttgtga tggctgtgca ataacaccct ctggtttttc gacacgatcg tcaccatcta
     2221 acatcgtaac cgggagcaca tcagccagaa ctgtattttt ataattggct tttgcttcaa
     2281 ttcccatgaa tgagagatag ccgccaatca tcaataaccc accaccgtta ttcacatatt
     2341 ctttaatcag ttcgagagcg tttggtttta tgcgaagttg ataaaaagta tcattttgta
     2401 gcaggaaagt attactacca atatcactaa taacgatggc atcatattgt tctaactgtg
     2461 ccacatcttc agggaaggca atttgtaccg tatgtgctgg catataggtg acatctacct
     2521 gactattttt cagacattgg agtaaccagg tcgcaccttc ttcatattta cttgaggtaa
     2581 aactgtcata tccttttgaa tgaatcatat gaatatgcca ggattcccca ataaagagca
     2641 cttttagtga ttttttggtg ttattcattc tctgtccctt cttaatgcgt aaaaatagtt
     2701 tgctgataat gtatactctg aattcgatgt atcacagatt catgttcagg catgtcggaa
     2761 gcattgcttg tctccaccgc tagggatgca aaggcgctgg cataacacag tgctgattcc
     2821 agtgattttc ctttcgcaag tcctgatgcc agagcgccgt taaaagcatc cccggctcct
     2881 gccgtatttt taacgaccgc tggaaacgct ggcgaatgaa tgaatttttt gccatcaaat
     2941 gcgagcgagc ccttgctacc gagcgtgata accgtgtttt ttaccccttt attgtggata
     3001 gcttctgcag ctcgtttagc tgattcaaga tcgtgtacgt ctatgccgga cagtaaacca
     3061 gcttctgttt cattgggggt gatataatca acatcctgaa taagttcgtt gacgatatca
     3121 ttatagggtg ccgggttaat aatgaccggg atactgtttt tttgcgccaa tgtgatcgct
     3181 tgttgcagag cggtataatt tgtttcgagc tgcagaagta tgacatcaga attaataatc
     3241 gcttctttct gaatagttat ttcatcggac gacattgtca tgttggcacc gggatagatc
     3301 gcaataacat tatcgccagt accttcatta acaaggatcg tggcagttcc ggtttgcgtt
     3361 tctttcgttt gataaattat acttttataa atcttagacg aattcataaa gttgatcgca
     3421 taatcactaa agtgatctgt acctactttt gttatgaaat aaacatcagt atcggcaaag
     3481 cttgctgcca gcgcttggtt acagcctttt ccccccggtg aaaatataaa attacttgcc
     3541 agaagcgatt ctccgatagt gggaagcctc ggcaaataac tgatgatatc aacattaaac
     3601 gaaccaataa cgcatacttg acttttcata gttgagtggc ctttttttat tccctgggct
     3661 gtatcataat tttcaagcgc ataagcgatt tcatttttcg caatagtatc aagcgagtct
     3721 aggacgataa aagcaccgcc atgacatcga gtcagatagc caagcctggc aagttcatta
     3781 agatctgatc gaatagtttc tttcgttaca ttgaggctat tggacagatc ggatactttt
     3841 acagaaccga atttactgag tcgtttcaat atttcatgat ggcgttcaaa tttcatgctt
     3901 cacctcattt cagttacgtt aatatctatc gatgtatatt gatgtgataa tcaccataaa
     3961 gtggcgggga aaagcaaaaa aaagcaaaaa tagcctgaga aaaggtaagc gttaatggag
     4021 aatcatattg atgcttattt attggtgaga actacgttcc atggcaggag ttcatcaata
     4081 cggttggaag gttattccgg cagtacgctc aggatatggc gcagatacgc ttccggatca
     4141 atgtcgttca gaaggcaagt gccgataagc ccatacaaca gtgcactgcg ctcgccgccg
     4201 aagaacataa agtttttatt tccgagacag atggcacaaa gggctttttc cgcggcatta
     4261 tgtctgcctc cgcctgaccg tcaacactgt aataacagag tgcgccccac tgattcagta
     4321 cacagctgaa ccattcaccc agtctggatt tttggatagc gtggcatttt tctccaccat
     4381 gcatgcagtg agtccagtaa cagtttgctc ttcttctgtc tgaccacaag atgtgctgga
     4441 ctggtaaacg atggggatac ctgcttacgc gagagcaata agtggctggc cacaattctc
     4501 ggtacaaaac tctgctgaat agcagcgatt ttcccagcgt ggtgcgtcgt ttgcatttta
     4561 ctggttctaa gttaacaatt atctgttcac taatgactac tctaaaaact tgtatcagga
     4621 ctatcactta tttaagtgat attggttgtc tggagattca gggggcctgt ctagagagat
     4681 ataaagatac cttcaccccg gtgatgataa aataaaagta tctcagctat taaactgatg
     4741 tacgattcgg gcaataactt attcgtatag agcacctttt ctctggtgct ctatacgaat
     4801 aagttattgc ccgaaattaa ttatattgtt tattatcaaa ccgagttata gcttttctct
     4861 gaaaactacc gaataaaata tactgataga ctgctgccta gaatttccag atcaggtggg
     4921 ctgcaagttg cagaccgtta taatcatcgg ttggggtgtt gaaccacact ttacctgccg
     4981 tcaacccgag attaagttgc tcagtcagcg gttgaatacg ttgcaaactg atgctcttca
     5041 tgctgccgta gcgtgtttct ggcgtccagt cgtaggtgta agcacctgtt ccgcggctta
     5101 gccatagttg cgt
//
LOCUS       DAWDRC010000268         5112 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7167_length_5112_cov_2.511964, whole genome shotgun sequence.
ACCESSION   DAWDRC010000268 DAWDRC010000000
VERSION     DAWDRC010000268.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7167_length_5112_cov_2.511964"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2990..2999
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1225 a         1417 c         1267 g         1193 t
ORIGIN      
        1 gtaacccggc atcaccgcgt cctgattgtt ctgcgcggtc tcgaccagag cggattgtaa
       61 ctgccggtta gccgtcagca actcgctaac ggtatctttg caccacagtt tcaggtcagt
      121 tgctacctga tcattacggc tacgcccggt atgcagtttt ttgcctaact gacccacttt
      181 gtcgatcagt ttgccttcca cccagctatg gatatcttcg gcgtcgcttt caaggatttg
      241 ttgtggcctg gcgcgaacat cttccagcag cacgttcagc gcctcttcca gttgcgcctg
      301 ctcttctgcg gttaacacgc cgaccgttac cagggctttg gaccaggcca cagagccaac
      361 aatatcctgc tccgccagac ggtaatcaaa gcgcagtgag tcgttgaatt gtttgaaccg
      421 ttgatctgct gcctgggtaa aacgcccgcc ccaaagtgcc ataactctgt ttccttattt
      481 tgaaattcaa tgccggaaag ctgcgcctcc ggccaacaaa acttaagcta aaatccgcgt
      541 acccatcggc ataccgttaa acagtgccgg aagctgttcc gcatgacgcc aggaggcgat
      601 atctaccgga cggcccaacg tgcgcgcagc atccagcgcc gcgttcactt tcacaatcat
      661 gccgtcagta ataatgccct gctcaatcag ttgttctgct ttcgcggcgg tcatttcggc
      721 aatacgttgc cctttgccgt cgagaatgcc gctgacgtca gagagcaaaa tcagatccgc
      781 gcccagcgtt gccgccagcg ccgtcgccgc ctggtcggca ttgacattca tcagttgccc
      841 ttcgtctgtt acgccaatgg agctgaccac cggcagataa ccgttctcca gcaaactgtt
      901 gataagctta ggcgaacctg gctgcgccag tccaacatgg cctaactctt catcaagctg
      961 ggtcactttg acgctgtcgc cgtcaccgag aaacaaacct acggccgcaa tctgatgttt
     1021 cttcgcccat gccagcaggg ttttatttgc cgttcccgcc agtgctccgg tgataatgtc
     1081 tatctgatcc gcaggcgtca cccgcaggcc atttttcttt ttcaccggca gattcagccc
     1141 tttcatcagc tcatccacca cgccgccgtg cacaatcacc agcggacgct gatgtgactc
     1201 gcgataattc accagtgcgc taaacagacg ttccagcgcc tcttcactat ccagcagtac
     1261 gccacccagt ttgataatta atggattcat cattgcaccc ttaaataaga gactgcgttt
     1321 ccgcatagcc gaaacgaata ttggcgcact gcaccgcttg tgccgccgcg cctttcagta
     1381 agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaaa ccgatatcgc
     1441 agaatggcag tccaacgaca tttttcagcg ccggaacgcc tttgtcatac agccgcacca
     1501 gtggtttatg ggcatacgcc tgctgtaacg cttgcgccac ttgcacctgg ctcacacccg
     1561 gtttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc agatgcgggg
     1621 tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga tgggtaaaga
     1681 cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc ttacgtcctg
     1741 caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca agaagatcgg
     1801 catcaatcaa cggtttcagc gccagctgtg ctgccgtcgg ataacagcct ggcacggcaa
     1861 tcaagttcgc ttcttttaat ttattaccgc accactccgc cagaccgtag gctgcctgtt
     1921 ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag gcgacatcgt
     1981 taacacgaaa cgcgccggaa aggtcgaaca ccacacagcc tgcttcaaga aattgcggcg
     2041 ctaaatcgtg gctgacttca tgggcggtgg cgagaaacac cacgtccacc cctgggctaa
     2101 actcgctgat atccgacatc ggctgcaacg gcagatcaac gatgcctttt agctgcggat
     2161 gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc aaagcggtta
     2221 tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg tagccgctgg
     2281 cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct ggttgccagg
     2341 ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac gttagtgtat
     2401 ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg tgtcaacaat
     2461 gaaaaacaaa ttaccgccat ttatcgagat ttaccgtgct ctgattgcca caccttcaat
     2521 aagcgccacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc tgctggcgga
     2581 ctggtttaag gatttaggct tcaatgtgga agtacagcct gtgccaggaa ctcgcaacaa
     2641 atttaatatg ctggccagtt gcggacaggg ggctggcggt ttgttgctgg cggggcatac
     2701 cgatacggtg ccatttgatg acggacgctg gacgcgcgat ccatttacgt tgacggagca
     2761 tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg cgtttatcct
     2821 tgatgcgcta cgcgatgtcg acgtcacgaa gcttgcaaaa ccgctctaca ttctggcgac
     2881 tgccgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta ccgccctgcg
     2941 cccggattgc gccatcattg gcgagccaac gtcgctgcaa ccggtacgcn nnnnnnnnnt
     3001 catatctcta acgccatccg tattcagggc cagtcgggcc actccagcga tccagcacgc
     3061 ggagttaacg ctatcgaact gatgcacgac gccatagggc atattttgca attgcgcgat
     3121 aacctgaaag aacgttatca ctacgacgcg tttaccgtgc cataccctac gctcaacctc
     3181 gggcatatcc acggcggcga tgcatctaac cgtatttgcg cttgttgtga gttgcatatg
     3241 gatattcgtc cgctgccagg catgacactc aatgaactta acggtttgct caacgatgca
     3301 ttggctccag tgagcgaacg ctggccgggt cgtctgacgg tcgacgagct gcatccgccg
     3361 atccctggct atgaatgccc accgaatcat caactggttg aagtggttga gaaattgctc
     3421 ggagcaaaga ccgaagtggt gaattactgt accgaagcgc cgtttattca aacgttatgc
     3481 ccgacgctgg ttctggggcc tggctcaatt aatcaggctc atcagcctga tgaatatctg
     3541 gagacgaggt ttatcaagcc cacccgcgaa ctgatcaccc aggtgattca ccatttttgc
     3601 tggcattaaa acgtaggcca aacaatgtcg gatgcgatgc ttgcgcatct tatccgacct
     3661 acacctttgg tgttattcga ggcgattttt taacattccc ataagttacg cttatttaaa
     3721 gcgtcgtgaa tttaatggcg taaattcctg ctatttattc gtttgctgaa gcgatttcgc
     3781 agcatttgac gtcaccgctt ttacgtggct ttataaaaga cgacgaaaag caaagcccga
     3841 gcatattcgc gccaatgcga cgtgaaggat acagggctat caaacgataa gatggggtgt
     3901 ctggggtaat atgaacgaac aatattccgc attgcgtagt aatgtcagta tgctcggcaa
     3961 agtgctggga gaaaccatca aggatgcgtt gggagaacac attcttgaac gcgtagaaac
     4021 tatccgtaag ttgtccaaat cttcacgcgc tggcaatgat gctaaccgcc aggagttgct
     4081 caccacctta caaaatttgt cgaacgacga gctgctgccc gttgcgcgtg cgtttagtca
     4141 gtttctgaac ctggccaaca ccgccgagca ataccacagc atttcgccga aaggcgaagc
     4201 tgccagcaac ccggaagtga tcgcccgcac cctgcgcaaa ctgaaaaacc agccggaact
     4261 gagcgaagac accataaaaa aagcagtgga atcgctgtcg ctggaactgg tcctcacggc
     4321 tcacccaacc gaaattaccc gtcgtacact gatccacaaa atggtggaag tgaacgcctg
     4381 cttaaaacag ctcgataaca aagatatcgc cgactacgaa cataaccagc tgatgcgccg
     4441 cctgcgccag ttgatcgccc agtcatggca taccgatgaa atccgtaaac tgcgcccaag
     4501 cccggtagat gaagccaaat ggggctttgc cgtagtagaa aacagcctgt ggcaaggcgt
     4561 accgaattac ctacgcgaac tgaacgaaca actggaagag aatctcggct acaaactgcc
     4621 cgtcgaattt gttccggtcc gttttacctc gtggatgggc ggtgaccgcg acggcaaccc
     4681 gaacgtcact gccgatatca cccgccacgt cctgctgctc agtcgctgga aagccaccga
     4741 tctgttcctg aaagatattc aggtgctggt ttctgaactg tcgatggttg aagccacccc
     4801 tgaactgctg gcgctggttg gcgaagaagg tgccgcagaa ccgtatcgct atctgatgaa
     4861 aaacctgcgt tctcgcctga tggcgacaca ggcatggctg gaagcgcgcc tgaaaggcga
     4921 agaactgcca aaaccagaag gcctgctgac acaaaacgaa gaactgtggg aaccgctcta
     4981 cgcttgctac cagtcacttc aggcgtgtgg catgggtatt atcgccaacg gcgatctgct
     5041 cgacaccctg cgccgcgtga aatgtttcgg cgtaccgctg gtgcgtattg ctatccgtca
     5101 ggagagcacc cc
//
LOCUS       DAWDRC010000269         5099 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7188_length_5099_cov_2.680611, whole genome shotgun sequence.
ACCESSION   DAWDRC010000269 DAWDRC010000000
VERSION     DAWDRC010000269.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7188_length_5099_cov_2.680611"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1189 a         1373 c         1293 g         1244 t
ORIGIN      
        1 aattacaaat attaataacg aagagatgac agaaaaattt tcattctgtg acagagaaaa
       61 agtagccgaa gatgacggtt tgtcacatgg agttggcagg atgtttgatt aaaaacataa
      121 caggaagaaa aatgccccgc ttacgcaggg catccattta ttactcaacc gttaccgatt
      181 ttgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcaacg tggtaagcca
      241 gtagttgcag cggaacggtg tagaagattg gtgcaatcac ctcttccaca tgcggcatct
      301 cgatgatgtg catgttatcg ctccccacaa aaccagcatc ctgatcggcg aagacataca
      361 actgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt
      421 tgttcggtgc aacgacgata accggcatat cggcatctat cagcgccagc ggaccgtgtt
      481 tcagttcgcc agcagcgtag gcttcagcgt gaatgtaaga gatctctttc agcttcaatg
      541 caccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg tgatgtttgt
      601 cagagaaatc ttctgccagc gcttcaatgc gtttgtcctg agacagcatc tgctcaatac
      661 ggctcggcag cgcctgcaga ccatgcacga tgtcatgttc aatggaggca tccagacctt
      721 tcaggcgaga cagtttcgcc accagcatca acagcacagt taactgggtg gtgaatgctt
      781 tggtggaggc cacgccaatt tccgtaccgg cgttggtcat cagcgccaga tcggattcgc
      841 gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt
      901 tcgacagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca
      961 tcaggctgtt acgacgcacg gcagatttgc gatagcggaa ttcagaggcg atttcgacgt
     1021 cgcacggaat acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag
     1081 aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttca tcggcgttcg
     1141 gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttga
     1201 tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg gcggtaaata cctttatcgc
     1261 ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat
     1321 cgaagatgtt taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa
     1381 agcgacgagt cactggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac
     1441 ccaggccaat caccagcgga ctaccagaac gtgccgccag cagggtatcc ggatgacggg
     1501 agtccatgat cactgtaccg tacgcaccac gcagttgcgg aatagcacgc agaactgcct
     1561 cacgcagagt accgccttgt ttcagctccc agttcaccaa atgtgcaatc acttcggtgt
     1621 cggtttcaga aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt
     1681 tttcgatgat gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttca
     1741 cttctgaagg ttcaccgtgg gtcgcccagc gagtatgagc aataccggtg ccgccatgca
     1801 gaggatgttc ttccgctgcc tgagccagca tctgtacttt accgaggcga cgcaggcggg
     1861 tcatatgacc ttctgcatct acaaccgcca gaccggcaga gtcatatccg cggtattcca
     1921 gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa
     1981 ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt
     2041 tttcgggcgc cccgagcctt gtagagagtg ggggtatttt tatagttact gcttgtgggc
     2101 gggagattat gttatctcct catcccatat gaccggatta cccggccaga attacttttt
     2161 ctttaccgga cgacgccagc cttctttttg agtctgcggc acacggctga tagccagggc
     2221 attttcgccg acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac
     2281 tgttaccggg gccaccagtt gagtgtcgga accgacaaac acatcgtcgc cgataatggt
     2341 cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt
     2401 atcgccaatt tccgcatcgc ccaggtaagt cagatgacca gctttcgagc ctttacccag
     2461 aagcgctttt ttcatctcaa cgaagttacc gacgtgtgca ccttccagca actcagcacc
     2521 aggacgcaga cgggcaaacg ggccaatagt acaggccgct gccagattcg catcttccac
     2581 gacggtatac ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc
     2641 ggcgccgatt ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc
     2701 aatttcaaca tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg
     2761 cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag
     2821 tcgggagagt tgcaggcggt tattcacgcc ttctacttcg cttaaacgtt gcggatgaac
     2881 ggcgacgatt tcacgtcctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc
     2941 gccctgagca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttggc
     3001 aatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg
     3061 ctcaacaatg ccggtaactt tgccgttttc acgggtgata cgtccataac cggtcggatc
     3121 atccagtttt accgtcagca gaccaatgcc cccctgcggt ttagcatcac gcagacgctg
     3181 gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc
     3241 ggcaaagaaa ggtgcggcct gctgcatcgc atgacccgta cccagctgtt ctgcctgaag
     3301 cacccagttc aggttgtcgt ctttcagcgc ctgcttaagc agatcgccgc cgtgaccata
     3361 cacaaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat
     3421 cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt
     3481 gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat
     3541 ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg
     3601 gacagaggat aaattgttca ataacggatt atccccgctg acgaaagcgc cattttcgac
     3661 catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg
     3721 gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacagggtgt tttatatcag
     3781 caggatctat gtgaacgctc ttcaggacgg gtcaacgcgt aaaaaaaagc cagcctgtta
     3841 ccagactggc ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata
     3901 acgcgcagct gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg
     3961 ccgtgagagc tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg
     4021 tcgagatcct ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact
     4081 tcaagaatgc cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga
     4141 atcataccag gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca
     4201 ccttcgctac ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg
     4261 ctgacgacgt ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt
     4321 ttttggcttt ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca
     4381 ggtgatcgta ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga
     4441 cgtatttacc cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc
     4501 gctggatctt acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac
     4561 ccaggatggc gataatgtct ttcagttcct gataacgttg caggatggac tgaacgccac
     4621 gcgcggtgtc gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt
     4681 ccagcgggtc aacggccggg tagataccca gagacgcgat ctgacggctc aataccacgg
     4741 ttgcgtcaag gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta
     4801 cgtatactgc ctgtacggag gtgatagaac cggttttggt ggaggtgata cgttcctgca
     4861 gaacgcccat ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca
     4921 gcagtgcgga tacttcggta ccggccaggg tgtaacgata gatgttgtca acgaacagca
     4981 gaacgtcacg accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc
     5041 gcagacggtt tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgata
//
LOCUS       DAWDRC010000270         5098 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7191_length_5098_cov_2.556811, whole genome shotgun sequence.
ACCESSION   DAWDRC010000270 DAWDRC010000000
VERSION     DAWDRC010000270.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7191_length_5098_cov_2.556811"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1098 a         1231 c         1373 g         1396 t
ORIGIN      
        1 aggattttgc agcgtaatat aattacggga tcaatttggt ttattcatta acacatttgt
       61 aactttttta tcattgtctt ttcctctttt gatgaaatgg caacgataga ggcgagcaaa
      121 taattttgta ttggggcatg cgtgtgaccc tttctaacgg ggttcactct cggagtcttc
      181 atgcgatgag caaggagtca tgatgttaga tatagtcgaa ctgtcgcgct tacagtttgc
      241 cttgaccgcg atgtaccact tcctttttgt gccactgacg ctcggtatgg cgttcctgct
      301 ggccattatg gaaacggtct acgtcctttc cggcaaacag atttataaag atatgaccaa
      361 gttctggggc aagttgtttg gtatcaactt tgctctgggt gtggctaccg gtctgaccat
      421 ggagttccag ttcgggacta actggtctta ctattcccac tatgtagggg atatcttcgg
      481 tgcgccgctg gcaatcgaag gtctgatggc cttcttcctc gaatccacct ttgtaggtct
      541 gttcttcttc ggttgggatc gtctgggtaa agttcagcat atgtgtgtca cctggctggt
      601 ggcgctcggt tcaaacctgt ccgcactgtg gattctggtt gcaaacggct ggatgcaaaa
      661 cccaatcgcg tccgatttca actttgaaac tatgcgtatg gagatggtga gcttctccga
      721 gctggtgctt aacccggttg ctcaggtgaa attcgttcac accgtagctt ctggttatgt
      781 gactggcgca atgttcatcc tcggtatcag cgcatggtat atgctgaaag gtcgtgactt
      841 cgccttcgct aaacgctcct ttgctatcgc tgccagcttc ggtatggctg ccgttctgtc
      901 tgttattgtt ctgggtgatg aatccggcta cgaaatgggc gacgtgcaga aaaccaaact
      961 ggctgctatc gaagccgagt gggaaacgca acctgcacct gctgccttta ctctgttcgg
     1021 cattcctgat caggaagagg aaacgaacaa atttgcgatc cagatccctt acgcgctggg
     1081 catcattgca acgcgttccg tggatactcc agttatcggt ctgaaagaac tgatggtgca
     1141 gcatgaagaa cgcattcgta acgggatgaa ggcgtactct ctgctcgaac aactgcgttc
     1201 tggttctact gaccaggccg ttcgtgacca gttcaatagc atgaagaaag atctcggtta
     1261 cggtctgctg ctgaaacgct atacgccaaa cgtggctgat gcgactgaag ctcagattca
     1321 acaggcaacc aaagactcca ttccgcgtgt agcgccgctg tactttgcgt tccgtatcat
     1381 ggtggcgtgt ggcttcctgc tgctggcaat catcgcgctc tctttctgga gtgtcatccg
     1441 aaaccgcatt ggcgagaaaa aatggctgct gcgcgccgcg ctgtacggta ttccgctgcc
     1501 ctggattgct gtagaagcgg gctggttcgt ggctgaatat ggccgccaac cgtgggccat
     1561 cggtgaagtg ctgccgacag ctgtggcgaa ctcgtcactg accgcaggcg atctcatctt
     1621 ctcgatggtg ctgatttgcg gcctgtatac cctgttcctg gtggcagaat tgttcttaat
     1681 gttcaagttt gcacgcctcg gcccaagcag cctgaaaacc ggtcgctatc actttgagca
     1741 gtcttccacg actactcagc cggcacgcta agacaggagt cgtcaaatga tcgattatga
     1801 agtattgcgt tttatctggt ggctgctggt tggcgttctg ctgattggtt ttgcagtcac
     1861 tgacggtttc gacatggggg tgggcatgct cacccgtttc ctcggtcgta acgacaccga
     1921 gcgtcgaatt atgattaact ccatcgcacc acactgggac ggtaaccagg tttggctgat
     1981 caccgcaggc ggcgcactgt ttgctgcctg gccgatggtc tatgccgctg cgttctccgg
     2041 cttctatgtg gcgatgatcc tcgtactggc gtctttgttc ttccgtccgg tcggttttga
     2101 ctaccgctcc aagattgaag aaacccgctg gcgtaacatg tgggactggg gcatcttcat
     2161 tggtagcttc gttccgccgc tggtgattgg tgtagcgttc ggtaacctgt tgcagggcgt
     2221 accattcaac gttgatgaat atctgcgtct gtactacacc ggtaacttct tccagctgct
     2281 taacccgttc ggcctgctgg caggtgtggt gagcgtaggg atgatcatta ctcagggcgc
     2341 aacctatctg caaatgcgta ccgtgggcga actgcatctg cgtacccgtg caacggctca
     2401 ggtggcggca ctggtgacgc tggtctgctt cgcactggct ggcgtatggg tgatgtacgg
     2461 tatcgatggt tatgtcgtga aatcgacaat ggaccattac gcagcctcta acccgctgaa
     2521 taaagaagtg gttcgtgaag cgggcgcatg gctggttaac ttcaacaaca cgccaattct
     2581 gtgggctatt ccggcactgg gtgtggttct gccgctgctg accatcctga ctgcacgtat
     2641 ggataaagcc gcgtgggcgt ttgtattctc ctccctgacg ctggcctgca tcatcctgac
     2701 tgccggtatc gcaatgttcc cgtttgtgat gccgtccagc accatgatga acgcaagtct
     2761 gacaatgtgg gatgcaactt ccagccagct gacgcttaac gtcatgacct gggttgcggt
     2821 ggttctggta ccgatcattc tgctctacac cgcctggtgt tactggaaaa tgttcggtcg
     2881 tatcaccaaa gaagatattg aacgtaacac ccactctctg tactaagtaa ggagctaaaa
     2941 atgtggtatt tcgcatggat tctgggaacg cttcttgcct gttcgtttgg ggtaatcacc
     3001 gcgctggcgc ttgagcacgt cgaatcaggc aaagccggtc aagaagacat ctgatgagta
     3061 agattatcgc aaccttgtat gcggtaatgg acaagcgccc cctgcgggcg ctttccttcg
     3121 tgatggcact tctgttagca ggatgtatgt tttgggaccc atcacgtttc gccgcgaaga
     3181 ccagtgaact ggaaatctgg catggtttac tgcttatgtg ggccgtctgt gctggtgtga
     3241 ttcacggcgt gggctttcgt ccgcagaagg ttctctggca agggattttt tgcccattgc
     3301 ttgccgatat tgttctcatt gtcgggctga ttttcttctt cttttaaatc agaattctct
     3361 ttaaaaattt atgggccgag tcaggcccat aaatttttac gctcccttaa cttgccctca
     3421 ttcccaaacc tcaatcgcgc gcgtatagta gcagcgttta aaagctctaa cttttgttgc
     3481 attaccggga tgtaaagtga atacaacgct gtttcgatgg ccggttcgcg tctactatga
     3541 agataccgat gccggtggtg tggtgtacca cgccagttac gtcgcttttt atgaaagagc
     3601 acgcacagag atgctgcgtc atcatcattt cagtcaacag gcgctgatgg ctgaacgcgt
     3661 tgcctttgtg gtacgtaaaa tgacggtgga atattacgca cctgcgcggc tcgacgatat
     3721 gctcgaaata cagactgaaa taacatcaat gcgtggcacc tctttggttt tcacgcaacg
     3781 tattgtcaac gccgagaata ctttgttgaa tgaagcagag gttctggttg tttgcgttga
     3841 cccactcaaa atgaagcctc gtgcgcttcc caagtctatt gtcgcggagg ttaagcagtg
     3901 actgacatga atatccttga tttgttcctg aaggctagcc ttctggttaa acttatcatg
     3961 ttgattttga ttggtttttc aatcgcatcc tgggccatta ttatccagcg gacccgtatt
     4021 cttaacgcag cggcgcgcga agccgaagcg tttgaagata aattctggtc tggaatcgaa
     4081 ctctctcgcc tctatcaaga gagccagggg aaacgggata atctgagcgg ttcggaacaa
     4141 atcttttaca gcggcttcaa agagtttgtg cgcctgcatc gtgccaatag ccatgcgccg
     4201 gaagccgtag tggaaggggc gtcgcgtgcg atgcgtattt ccatgaatcg tgaacttgaa
     4261 aatctggaaa cgcacattcc tttcctcggt acggttggct ccatcagccc gtatattggt
     4321 ctgtttggta cggtctgggg gatcatgcac gcctttatcg ccctcggggc ggtaaaacaa
     4381 gcaacactgc aaatggttgc gcccggtatc gcagaagcgt tgattgcgac ggcaattggt
     4441 ttgttcgccg caattccggc ggtaatggct tataaccgcc tcaaccagcg cgtaaacaaa
     4501 ctggaactga attacgacaa ctttatggaa gagtttaccg cgattctgca ccgccaggcg
     4561 tttaccgtta gcgagagcaa caaggggtaa gccatggcca gagcgcgtgg acgaggtcgt
     4621 cgcgatctca agtccgaaat caacattgta ccgttgctgg acgtactgct ggtgctgttg
     4681 ctgatcttta tggcgacagc gcccatcatc acccagagcg tggaggtcga tctgccagac
     4741 gctactgaat cacaggcggt gagcagtaat gataatccgc cagtgattgt tgaagtgtct
     4801 ggtattggtc agtacaccgt ggtggttgag aaagatcgcc tggagcgttt accaccagag
     4861 caggtggtgg cggaagtgtc cagccgtttc aaggccaacc ctaaaacggt ctttctgatc
     4921 ggtggtgcaa aagatgtgcc ttacgatgaa ataattaaag cactgaactt gttacatagt
     4981 gcgggtgtga aatcggttgg tttaatgacg cagcctatct aaacatctgc gtttcccttg
     5041 cttgaaagag agcgggtaac aggcgaacag tttttggaaa ccgagagtgt caaaggca
//
LOCUS       DAWDRC010000271         5082 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7225_length_5082_cov_2.977720, whole genome shotgun sequence.
ACCESSION   DAWDRC010000271 DAWDRC010000000
VERSION     DAWDRC010000271.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7225_length_5082_cov_2.977720"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1307 a         1262 c         1176 g         1337 t
ORIGIN      
        1 cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa accgaaggta
       61 cggaagccgg agttttccag cgccacgtta cccgcgagga taaacaggtc ggcccaggag
      121 attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt atcgaggctt
      181 acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc cgcgccgccg
      241 cgaccgtcga ttgaacggta agtacccgcg ccgtgccagg ccatacgaat aaacagaccg
      301 gcgtagctgc cccagtcggc tggccaccac ggttgagatt ctgtcagcag ggctttcaga
      361 tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc aaagtcctca
      421 cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag ttgatttggc
      481 caccagtcgc gcgtggttgt gcccccacct gcgctctggt cgtgaccgcc ttgatggaac
      541 gggcatttgc cggtggctgt cgtgttatag atatcgtctg acgtgctcat caatgtgctc
      601 ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa ttgaattaat
      661 ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta gatcaaattg
      721 atcgtaatta agactttttg attatgtccg atttaggata aatgctttat aaaaaagggt
      781 tttagatttg tatccttcaa gtaatgaggg agaaaacaag gctgataacc ttattttccc
      841 gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac tgtgtgattt
      901 tcacaaaagg cacactattt ataaaccagg tcgaacaccc agtgtatggc aaatcgcgta
      961 actcatttca gcgcggttaa gcgtatagaa gtggaaatct ttcactcctt cacggcttaa
     1021 aatcttcacc atatccatgg caatattcgc gcccaccagc ttgcgggttt cagcatcgtc
     1081 atccaggccg tcgaacattt gtgccatcca cgccggaata cgcacgttgg tcatgtcggc
     1141 aaatttcttc gcctgtttaa agttcgaaac cggcaaaatt cccggaatga tttccacatc
     1201 aatgcctgcc gagacgcagc gatcgcgaaa acgcaggtag ctttcgacat cgaagaagaa
     1261 ctgagtaatg gcgcggttgg ctccggcatc cacttttcgt ctcaggttga gtaaatcggc
     1321 ctgggcactt ttcgcttctg gatgtacttc cggatacgcc gccacggaga tatcgaaatc
     1381 tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct taccacttcc
     1441 cggcggcagg tcgccacgca gcgccacgat atggcgaata ccgttattcc agtagtcgcg
     1501 tgcaatgatc cgcagctcgg caggcgtcgc atcaatgcag gtaagatgcg gtgccgcttc
     1561 caggccggtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc gctcgccgga
     1621 gtttgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc gatcgatgga
     1681 gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaattcga acgaaacgtt
     1741 aatctgcccc tggacttctg ccagactctg attcagggca tcccgctggc tggcgtgaaa
     1801 aaagctcata ccttacctca tcaatcgctt atcgttatat gttgtgttgt gcacatctat
     1861 ccgtttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa ccgaaaaata
     1921 accaatgatg gtgaggaaaa atcagggaag atgaaaaaaa ctcatcttaa ttagaggcga
     1981 taaaagattt ctctatagcg caatccacct cgcacgtttt gactcatatt ctgacaacac
     2041 agcgacacct gggctaattc taaaaacaac gtcctgccac cggatggtga tgctaaggca
     2101 tctgattcgg cctgcgccct cgcgtaggcc gaatacagga tctgcgcaat caacgggtaa
     2161 caaacggttg agtcaattca gtgcgcactt ttatttgata tccttcacgc gcataccgtt
     2221 gagctgttca tccgtaatgg tgttgcccgc tttgaagtaa tcaaccaccg cgtgataaac
     2281 gtaataaccg cccgttgtgt tacgcgcttt cccttcggta aaggcggtaa aaccatctcc
     2341 accatcagca aggaaactct gagtagcaat gtgataaacc gtcgcatctt caatgggttt
     2401 gccattcaga gtcagtgtga ttacccgctg accaaccggc ttgctgctgt cgtacttcat
     2461 ttccaggcct ttcgatacct gtaaaacgcc attgcttaaa cttgcgccgt gttccatcag
     2521 actgcgtaat tgtttacccg tgagttccat cgtcaccagt tcgttgggga acgggaacgt
     2581 actgatgacg ccgcccatcg tgatcgcccc tgcggggatc tcattgcgga taccaccaga
     2641 gtttgttaaa gccagttggg tatttttacc cgctgctgcc agtaaagcgt cagccgccag
     2701 gttcccgagg gaagccgatt caccataggc acgttttaat tcaaccggcg attgcgccac
     2761 cgtttgttgc accacttcat caagcttttt attccagccg tcgatcacct gtttcgtttg
     2821 cggatcgggc ttccactcgt cagcgtaaat ggttttaagc tcgaagtttt tcaccgtaaa
     2881 attatgcggc ttctctttgt agtcgagaac cagtttgccc acatcaatcc cgccgctgtc
     2941 agtggagagg atgagcgtat tgccgacttt aatcggttcc ggcgtcccca catgtgcatg
     3001 tccggtgatc aaaatatcca gccctttcac ctgacttgcc gtctgaatat ctttatccaa
     3061 cgcgcgacgc acatctgtgc ctcccatact ggactggcgg gccggaacac cttcgtggat
     3121 cagggcgacg gtcaaatcaa ccttgccttt gagttcatcg atataacgtt gtagccattt
     3181 aatttcatcg cgcgcttcaa tacccactcg cgtggcagca gataccgtat cattgaaggc
     3241 aaatactccg tgcaaaccta taacgccaat tttcacgccg tctttttcga tgatggtata
     3301 tggcttatcc cagaatgatt tactgctgtt ctgataaaaa atattgccct gcacaatagg
     3361 gaattttgcc tggctcaact gtaataatgt gttgtcccag ccgtggtcaa attcatggtt
     3421 gcctattgtg acggcatcaa acggcatagt attcataata tcgataatag ccttaccttt
     3481 ggtcaggctg ctgatatatg gcccggtaaa atagtcgccc gcatcaaaaa accatgtcgc
     3541 tttatttttt gctttttctt gcttaaccag cgtagtgata tttgcccagc cgccaatatc
     3601 ccgtttaccg tcagcgatcc acggcacttt ataaggttct acatgcgcat ggaggtcgtt
     3661 ggtataaatg atagtgacat ctttagcgca agcccagaac ggcagtgtta atgcgatacc
     3721 cgcagcaagt attttaattt tcataaacaa tccctttcag tgagataaag gattgctaca
     3781 ttacgttctg aaagacttat ttatgtgaga gtaatcacat ccgcagggga tagttgccgg
     3841 acaaaatcaa cgaccttgtg aaatcgttgg tgatatatgt cgactctgtt tacgattaat
     3901 gcctgtaaaa cgttcggttg ccgaaatttg ggattggcct catcggaaga ttacagttgg
     3961 ccagactata aactgggtta tccggcactg cactgtcggg catgtggcag ttacccgccg
     4021 ttgtttgacg agcaacaatt tcgtgactgg ttgtcagttc acttgtctac ttatgcaatt
     4081 gagaaaggcc atttctgccc ggtatgctat ggcacagaca tgatttgtta tggtcacaat
     4141 ccacagggtt cgcaacgcat tcaatgccgt aactgcaaaa aagtctggac gcctaaaaaa
     4201 tatcagaaag aaattacgca tccccaggcc attgaaaccg ttcaactgtt catacccttt
     4261 caaggggcca gcgcagtaca aaaactgtat gttttagtca gtcttgacgc cactcgtggc
     4321 aacattcttc atctctccac gaattatacg caacatcaga caggagacag tctgcggtac
     4381 agttacaagg gcaacacaga accaactatg caccatcgcg atatcgtgca gaaggtagat
     4441 atgcgtgaag cgcaattctt gcgccggagc cagttcgatg aaattcagta tggcagtgct
     4501 gtactcaagc gcaacggtaa gggagccata ctacgcccgg ttatcacggc acacgggcat
     4561 ttcagaatac tgaaaatccg ctatccagat gtcaaaacgc atattatttc acacgaatgt
     4621 tttctaagag gcgcaattat tacagcctgg gcagatcagt tccgccaaca acaaggtgaa
     4681 ctttggttcg tagaagaaga aatcagtgac agtaacgctg atacgccctg gcattttaag
     4741 ggaacgacat accatggttg gtggcaaaat cagtggcagc gctgggagca gaggaataac
     4801 tgcaagatgg tctgcctact cacgggagcc agcttagaaa ggggcgcaaa tgtttctctg
     4861 gcaaccagtc gttgttttat cacatggctg acagaccaac acgactttac ccaaagcgcg
     4921 ttattatccg caggtcgcgt aacgcagatg ctgacctcac tggcgttaaa atacaatgaa
     4981 tcgctcactc catcttgtta gtcaatatac tcgacaaacg accataaatt aagagagatc
     5041 actacctttc atttaattaa atatataaat gtatgaccgt ga
//
LOCUS       DAWDRC010000272         5072 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7236_length_5072_cov_2.058999, whole genome shotgun sequence.
ACCESSION   DAWDRC010000272 DAWDRC010000000
VERSION     DAWDRC010000272.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7236_length_5072_cov_2.058999"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    693..702
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2264..2273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2981..2990
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1145 a         1482 c         1264 g         1151 t
ORIGIN      
        1 acaccagcga gaaatgcaga ccggggcgat aaattttgcg gccaaagtag ccaaacagca
       61 gcgttctcac cacactattg accagataac cgagaatcgc ggtcatcgcc agcggccaga
      121 aatgggcgct aaccaccgta aaagtaaagc ccgccagcac cgcgctggtt tcgatcatgc
      181 cgattttgtt gaactccagc tccttttgca tcaacgcgcg gaactgttgc ccgtggggga
      241 tcaccacaaa cgccagcgac aatgttttaa tcaacggtgc caggtccggg ttattcagca
      301 agtcgccgat gagatcactc aacaaaaaca ccgccacgca caccacgatc cccagcccga
      361 cgttcagcca gtacagcgtg gtgagttcaa ggtggctgat ttcttttcgc tgaataatgg
      421 agttagcgat accgaagtca gaaagcgtat ctgccagcgc gatgatcacc agcgacacgg
      481 taagcaaacc gaactggtgg ttatctataa tccgcgccag cacagtcatt tgcaccagac
      541 cgaggccgat gatgatcacc gtggcaatcg ccgaccactt cgcgccgctg atggtttttt
      601 cacgtaagct catatcaata tgccgctttg ttaacgaagc ccttgaatac cgtcaggaaa
      661 acgattttga tatcgaacca gacgctccat tcnnnnnnnn nnggatgtac tcaaggtcga
      721 actcgacgcg tttttccatt ttctccagcg tgtcggtttc gccgcgccag ccgttaatct
      781 gcgcccagcc ggtaatgccc ggtttcactt tatggcgcag catgtagcct tcaatgagct
      841 ggcgatactg ttcgttatgc gccaccgcgt gcggacgcgg accgacaatc gacatcccgc
      901 cagtcagcac attgataaac tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc
      961 ccactttggt gacgcgcgga tcgttctgcg tcgcctgggt caccactttg tcgttctcca
     1021 tcactttcat cgaacggaac ttccacactt tgatcggctt gccatccatg ccatagcgag
     1081 tctggcggaa aataaccggt cctggtgaac tgagttttac cgccagcgca atacagcaca
     1141 gcaccgggga gatcagcagc agaataagcg tcgccagcac aatatcttcc gcacgtttta
     1201 gcaggcggtt aaccccggaa agcggcgtgt catacagcgg caccaccgga acgccgttca
     1261 tctcttcgat acgggaatgg agaatgttga aggtaaagac atcggggatc agcagtaccg
     1321 aacaggtggt gtccgccagt tgatggacca gttttttcac gcgcgcgccg tcgcacatct
     1381 gcatcgcgat atagacgtta tgaatcttgc ccgctttcgc atcctcgacc agttgttgca
     1441 gattgcccgc ccagtcgtta gaaacgccac ccggtttcgg gtcgtggtaa acacccacta
     1501 cttcaaaccc taaccacggc tgattacgga agctctccat cagcatttgt ccggcggcta
     1561 aatcccccgc tacggcgacc atacgcttgt tatagccatg attacgcagc cagcccgccc
     1621 caatgcgaat acacgaacgg caaaccacca gtccgatgct ggtcagccca taccacgcca
     1681 gccagatttt caattgcgtg tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa
     1741 ttacgcttaa cgtccagttt tgtagcaaca gagcaaattc tgtcgctgcc cgaacaccgc
     1801 gccatgagcg ataaaaatcg gtgatgccgc ccaacatctg gaacaccacc agcgtaatca
     1861 gtgccaccaa caggtgcatg tagaggaatg acagtccgct cgcttcgcaa accagccata
     1921 gtccggcaaa catgatggtg atatctgaaa agcgttgcac catagagatt aacgatgcat
     1981 tggttttcgc tcgctcgcgc ttttttagat ttgtcatcgt tgttcctgtt tttagctcct
     2041 tacccgcagt ggggtaaggg aagatccgac attactcgtt cagcaacgtc agcagagttc
     2101 gcgttcgcgc ttccatcagc ggcacatcac cacgcgattc cacattcaac cgcaccaccg
     2161 gttcggtgtt ggaggagcgc agattaaagc gccagtcggc aaaggtcatg ctgatgccat
     2221 cggtacgatc caccgccagc gcctcgcggc taaaatgctg ttcnnnnnnn nnnacgcggt
     2281 taatcgcctc atcggggtac gccagtttgc tgttgatctc accgcttgcc gggaacgccg
     2341 ccatccggtc gcgcaccagt tcgcccagcg tttttccttt caggcacacc agttcggcta
     2401 ccagcagcca cgggatcatg ccgctgtcac agtaagcgaa atcacggaag taatggtggg
     2461 cgctcatttc gccaccgtag atagcgtctt ccttgcgcat acgttcttta ataaaggcgt
     2521 gtccggtttt cgacattacc ggggtgccgc ccgcggccgt caccacatca atggtgttcc
     2581 aggagagacg tggatcgtgg atgatcttcg cgccgggatt tttttcgagg aacgcttctg
     2641 ccagcaggcc gacaatgtag taaccctcga taaactgccc tttttcgtca aacaggaaac
     2701 agcggtcaaa atcgccatca aaggcaatgc ccatatccgc gccgtgtttg atgaccgcat
     2761 tgcgggtgtc gtcgcgacat tccggcagca gcgggttagg aataccgttg gggaaattgc
     2821 cgtccggcgt gttgtgcact ttgatcaatt ccacaggtgc gccgagggct ttaaagcggg
     2881 cttcgatagc gtccaccacc ggacccgctg cgccgttccc ggagttgatc accagcttga
     2941 gcggcgtaag gtttttgaaa ttgatataac cgaacaggtg nnnnnnnnnn gtgatcaacg
     3001 taagcgtcac gcagattgat ttgctgatag cgaccgcgtt tggtttcatc gacgggagga
     3061 aagtcgttgg cttctgccag acgctggaca tcgcgcagtc cggtatcgcc gctgatcggg
     3121 cgagcccctt cgcgcaccag cttcatgccg ttgtaatcca tcgggttatg gctggcggta
     3181 acttcaatgc cgccatccac gccgagatgg aacgtggcga aatagatttc ttcggtgccg
     3241 gacatgccga tatccagcac gtcgacgccc gcatcctgta aacctttcgc cagcgccagt
     3301 tttaaggttt cgctggtgag gcggacatca ccgcctaaca caatggtttt cggtttgaga
     3361 aattcgccat aggcgcgacc aatgcgccag gcgatatctt cattcagttc ttcgcctaat
     3421 ttcccgcgaa tatcataggc tttaaagcag gttaattttt tcatatcgtt accctttttc
     3481 aggcaatagt tggccctgac cgaaacggcc aattttgttt ttgtcgttat ttacatcgcg
     3541 gtttcgcatt cattgcctga tgggacgctg ccgcgtctta tcaggcctac aaacccgaac
     3601 cgtaggtcgg ataaggcgtt cacaccgcat ccgacaaaca gcgcctgatg cgacgtttac
     3661 acccgtccgt agcgatccgc gaagcgcacc acatcatcct cttcgagata agagccggag
     3721 cgcacttcaa ttaaatcgag cggaattttc cccgggtttt ccaggcaatg cgtcgccccc
     3781 agcggaatat aaatggactc gttttcacca agcagtttga tatcaccgtc gatagtgact
     3841 tttgccgttc ccgcgacaac cacccagtgt tccgcgcggt gatggtgcat ctgtaccgac
     3901 aagccttcgc ccggtttcac ggtgatgcgt ttcacctggt agcggtcgcc cgcgtcgata
     3961 gagtcatatt tgccccacgg acgatacact tcgcgatgca cccgatgctc atggcgacca
     4021 tcagctttga tctgctcgac cacttttttc acatcctgca ccgcgttacg gtcggcaatc
     4081 agcactgcat ctttggtctg cactaccacc aaatctttca ccccgacggt ggtgaccagg
     4141 ccagattcgg catacacata gctgttttca gttttgtgat taatcacatc gccatggcaa
     4201 acgttgccct cggcggtgtg ggcactgatc tcccataaag aggaccagga accgacatcg
     4261 ctccagcccg catccatcgg caccacaacg gcatctgccg tgcgttccat gaccgcgtaa
     4321 tctaccgact cttccggaca ggcgagaaac gcctcttcat ccacacgaat aaaatcgaga
     4381 tccggatcga cggcgctcat cgctttttca caggcatcga gaatatccgg acgatacttt
     4441 ttcagttctt cgagataacg tccggcacgg aacaggaaca taccgctgtt ccagtaatat
     4501 tcgccacttg ccacataggc ctgcgcggtt tccagattcg gtttttcgac aaactgcgcc
     4561 acttcaaagg ccaccgtatc ctgctcaccc gccgacactt caccgcgacg aatatagcca
     4621 tatccggttt caggtagatc cggcacaatg ccgaaggtca ccagcttgcc cgcttcggca
     4681 tacggcatgg cattacgcac ggcggcacgg aacgcgtctt catcggcaat cacatgatcc
     4741 gccgccagca ccagcattaa tgggtcgttc tccgggctat gacgttttgc cgccagcgcc
     4801 gccagcgcaa tggccggtgc agtgttacgc cctgccggtt cgagaataat gttctcggtg
     4861 agtttattta gttgacgcag ctgttccgcg acaataaagc ggtgctgctc attgcaaatc
     4921 accaccgggc tttcgcactc cacgccgttc aggcggcaga tggtggtttg cagcatggtg
     4981 agatcgcctt tcaggcataa aaactgcttg gggtaaagta cgcgggaaag cggccataag
     5041 cggctaccgg agccacctgc catcacaact gg
//
LOCUS       DAWDRC010000273         5070 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7242_length_5070_cov_2.341974, whole genome shotgun sequence.
ACCESSION   DAWDRC010000273 DAWDRC010000000
VERSION     DAWDRC010000273.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7242_length_5070_cov_2.341974"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    686..695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3653..3662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4717..4726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1307 a         1259 c         1196 g         1278 t
ORIGIN      
        1 cgtgaagagt tagatgagtt tccggcaagc gaaaaagagt ttaccagcgc cagagagctg
       61 ggcgtttcga tcatcgatgg atttacacca gtagccgtgg aaggcaataa agtcaccttt
      121 aagcatgttc gtttaccagg tgaactaacg atagcggcgg acaaaattat tctcgctgta
      181 ggacaacatg ccgaacttgc tgttttttcc gcactggaac cgcagcgtaa caccatcgaa
      241 acgcaacatt accagacccg cgacccgcaa gtctttgctg ctggcgatat tgttgagggt
      301 gacaaaaccg tggtctatgc agtgaaaacc gggaaagaag ccgccggggc gattcatcac
      361 tatttagagg gagcttgctc atgttaacga aagatctttc gattactttt tgcggcgtga
      421 agtttcccaa cccgttctgc ctttcttctt cgccggtagg caactgctat gagatgtgcg
      481 ccaaagccta cgacacaggt tggggcggcg tggtgtttaa aacgatcggt ttttttatcg
      541 ccaacgaagt ctcgccgcgt tttgatcatc tggtgaaaga agataccggt tttatcggct
      601 tcaaaaatat ggagcagatt gctgaacatc cgttggaaga gaatctggcc gccctgcgtc
      661 ggctgaaaga agattacccg gacaannnnn nnnnncatgg gcgaaaatgc gcagcaatgg
      721 gaagagctgg cgcgtctggt gcaagaagct ggcgcggata tgatcgagtg taacttctcc
      781 tgtccgcaaa tgacttctca tgcgatgggt agcgatgtcg ggcaaagccc ggagctggta
      841 gaaaaatatt gtcgggcagt aaaacgtggt tccacgctgc caatgctggc gaagatgacg
      901 ccgaatattg gtgatatgtg cgaagtggcg ctggcggcga agcgcggcgg cgcagatggc
      961 attgcggcga ttaacaccgt taaatccatc accaatatcg atcttaatca gaaaattggt
     1021 atgccgatcg ttaacggtaa atcgagtatt tccggatatt ccggtaaagc ggtaaaaccg
     1081 atcgccctac gctttattca gcaaatgcgc acccatccag aactgcgcga tttcccgatc
     1141 agcggtatcg gcggcattga aacctgggaa gatgcggcgg agtttttatt gctcggcgca
     1201 gcaacgttgc aggtgaccac cggcattatg cagtacgggt atcggatagt ggaagatatg
     1261 gcaagcgggt tgtcgcatta tctcgccgat caagggtttg attcgttgca ggagatggtg
     1321 ggtctggcga atcacaatat tgtcccggcg gaagatttag accgcagtta tattgtctat
     1381 ccccatatca atcttgataa atgtgttggc tgtggacgct gttatatttc ctgttacgac
     1441 ggcggtcacc aggcgatgga atggagcgag aaaacccgca caccgcattg taataccgag
     1501 aaatgcgtgg gttgtctgct ttgtggtcat gtctgcccgg taggttgtat tgatcttggg
     1561 gaagtgaagt ttaagaaagg cgagaaagaa cacccggtaa cgttgtaaaa ccagcgacgc
     1621 atccggcatt tagttcctgt cgccggatgc ggcgtgaacg ccttacccgg catacaaaac
     1681 aatataaatc agtatgttgt gctcattgta ggccggataa aacgcgccag cgttgcatcc
     1741 ggcatttggt tcctgtcgcc ggatgcggcg taaacgcctt atccggcata caaaaccata
     1801 caactcatga tgttatgtgc atttctgacc ggatgcgaac cacattcaca tcacttctta
     1861 cgcgcgtatt tcagtgaatc cagcgccacg gcgaagataa taatcgcccc tttgatgatg
     1921 tactgccagt acgggtttac gccgatgtac gtcaggccat agttgatgac ggtaaaaata
     1981 atcaccccgg tcacaacacc aattaccgtc cccacaccgc cgctgaacga tacgccgcct
     2041 accacgcacg ccgcgatagc atccagctca tacataaagc cgaggttgtt ggtggctgag
     2101 ccgatacgtc cggcttctaa catcccgccg aaggcataga acacaccaga caacgcgtaa
     2161 atcatcagca ggttcaggcc gacgttgaca ccagatactt tcgccgcttc cgggttaccg
     2221 ccaatggcaa aaatgttctt accgaagcgg gttttgttcc ataacaccca gacaaacacc
     2281 accgcaatca acgcatagaa ggtgatgtaa gagagacgga aactccccag cgcaataaag
     2341 ccttgggcaa aagtggagaa accactgtca aaaccggaaa ttggcgatgc cccgacaaag
     2401 tcgtaataga gcgagttaat gccgtagacg ataatcatcg taccgagcgt ggtaatgaac
     2461 ggcgtcacgt tgagataggc gataatcaag ccgttgatca aaccgatcac cgcaccaatg
     2521 gcacagacaa tcagaataac cagcgcaatc ggcatcgtcg ccatttccgg gaacactttg
     2581 ttggcgttat ccatggactg caataacgtc gctgctacta ctgccgccag ccccacctgg
     2641 cgacctgcgg aaaggtcagt cccctgggtg acaattaacc ctgccacacc aagcgcgata
     2701 ataatacgca ccgatgactg ggtgagaata ttacttaagt tcaacagact taaaaatgtt
     2761 gggtcctgga aaataataat cgccagcaaa actaaaagaa cgacgtaaat accgccctct
     2821 ttcaggtaag taagaaaact tttcttattt aatgcactca tgggaagccc ctaatcttaa
     2881 aggtgcaaag acgcaagacg cagaatttcg ctttgcgttg ttgtttttgt atcgacaatt
     2941 ccggaaacga gaccattgct cataaccaga atacggtctg ttatccctaa caactctggc
     3001 atttcggagg agataataat aatccccttg cctttcttcg ccagttcggc aattaactga
     3061 taaatctcaa acttagcccc aacatcaata ccgcgcgtcg gttcatcgag cattaatatt
     3121 tctggttgcg ttaacagcca gcgaccaata atcacttttt gctgattacc accggagagc
     3181 gaaccaattt gcgtccgatg ccccggggtt tttacccgca tcgaatcaat cacccactgg
     3241 gtatcgcttt tcatccgcga gttatccagt aaaccaactt tatttttata gttgcggata
     3301 ttggaaatta acgagttaaa gccaatatcc agataggcat aaattcccgt tgagcggcgc
     3361 tcttcagtta ccagtgcaaa tccgtggttt atggcttcgt tggcattatg gttattgatc
     3421 tttttgccgt gcaacgtaat ggtgccagcc gatttctcgc gaataccaaa taacgtctca
     3481 acaatatcgg tacgtttcgc tcccaccaga ccggctatac cgagaatctc ccctttatgc
     3541 agatcaaacg agacatcgcg tatcgacggc tggcgcagcg acgtcaggtt acgcacttcc
     3601 agaatcactt cgcccggctt gttttctttg tcagggaaac gctggttaag agnnnnnnnn
     3661 nngaacgccc aaccatcatg gcgatgatct tgtccatcgt cagtcctgcc agcggttcgg
     3721 tagcgatcca ctgaccgtcg cgcaatacgg taacttcatc acataactgg aagatttctt
     3781 ccattttatg cgagatataa acaataccgc agccgcgctc ttttaattta cgaataatag
     3841 tgaacagatg attgacctct ttttcggtta acgaagaagt cggttcatcc ataatcacaa
     3901 ttttcgcgtt ataggaaaac gctttggcga tttcgatcat ctgcatttgc gaaacagata
     3961 atgtaccgac gcgcgcacgc ggatcgatat caatatccag ttcatcaaaa atcgctttgg
     4021 tttcgcggta cattttgtcc tgatcgacaa acatgccttt ggtgggatat cgccccagcc
     4081 acatgttgtc catcaccgaa cgttgtaata ccaggtttaa ctcctgatgt accatcgaaa
     4141 taccattttc cagtgcttct ttggcggaat ggaaatcgat ctctttaccc tggaataaaa
     4201 tggtgccgga gtctttttga taaataccaa acaggcattt taataatgtc gattttcctg
     4261 caccgttttc ccccattaat gcatggatag aatgtggtcg gacttttaaa ttaacgttat
     4321 caagtgcctt aacaccagga aaagacttgt tgataccgct catttccaac aagtattccc
     4381 cggaagacgg agtcgttgag ctgaccatat aattttacct tgttggccat acaataaggg
     4441 cgcagtaaac gactgcgccc aatcagtctt atttcttgct gaattcagcc aggttatctt
     4501 tatctacgcc aacataaggt acgcggacca ctttgttgtc gattttccag ttggtgccat
     4561 cagccgcacc tttgccatcg gccaggtttt tcgccagatc aaaggtcgct ttcgcctggt
     4621 tgttagcatc gttcagtacg gtgcccgcca gtgcaccgga tttcaccagc gccagcgctt
     4681 ctggcagcgc atcgacgcca aacaccggaa tgctggnnnn nnnnnnctgg acttgttgtg
     4741 tgctttcagt gcttctaccg cgcccattgc catcgcgtcg ttgttggcga taaccacctc
     4801 gattttgttg gcgttcgggc cagacagcca ggcgtccatc ttatctttcg cctgagcggt
     4861 atcccacata gcggtatcta actgtaactg ttcagtttta atgcctttgt cgttcaattc
     4921 tttaatcacg taagtggtac gtgcttctgc atctggatgc cccggctcac ctttcagcag
     4981 tacgaattga atctgaccgt ctttgttcag atcccaaccc tgattcgccg cccagtgttt
     5041 agcaatcaaa tcgccctgaa taatgccgga
//
LOCUS       DAWDRC010000274         5041 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7284_length_5041_cov_2.734858, whole genome shotgun sequence.
ACCESSION   DAWDRC010000274 DAWDRC010000000
VERSION     DAWDRC010000274.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7284_length_5041_cov_2.734858"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2663..2672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3701..3710
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4341..4350
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1196 a         1395 c         1259 g         1161 t
ORIGIN      
        1 acggcgatct gctcgacacc ctgcgccgcg tgaaatgttt cggcgtaccg ctggggcgta
       61 ttgatatccg tcaggagagc acccgccata ccgaagcgct gggcgagctg acccgctacc
      121 tcggtatcgg cgactacgag agctggtcag aagccgacaa acaggcgttc ctgatccgcg
      181 aactgaactc caaacgtccg cttctaccgc gcaactggca accaagcgcc gaaacgcgcg
      241 aagtgctcga tacctgccag gtgattgccg aagcgccgca aggctccatt gccgcctacg
      301 tgatctcgat ggcgaaaacg ccgtccgacg tgctggctgt ccacctgctg ctgaaagaag
      361 cgggtatcgg gtttgcgatg ccggttgctc cgctgtttga aaccctcgat gacctgaaca
      421 acgccaacga tgtcatgacc cagctgctga atatcgactg gtatcgcggc ctgattcagg
      481 gcaaacagat ggtgatgatt ggctattccg actcagcaaa agatgcgggc gtgatggcag
      541 cttcctgggc gcaatatcag gcacaggatg cattaatcaa aacctgcgaa aaagcgggta
      601 ttgagctgac gttgttccac ggtcgcggcg gttccattgg tcgcggcggc gcacctgccc
      661 atgcggcgct gctgtcacaa ccgccaggaa gcctgaaagg tggcctgcgc gtgaccgaac
      721 agggcgagat gatccgcttt aaatatggtc tgccagaaat caccgtcagc agcctgtcgc
      781 tgtacaccgg ggcgattctg gaagccaacc tgctgccacc gcctgagccg aaagagagct
      841 ggcgtcgtat tatggatgaa ctgtctgtta tctcttgcga tgtctaccgc ggctacgtac
      901 gtgaaaacaa agattttgtg ccttacttcc gctccgctac gccggaacaa gaattgggta
      961 aactgccgtt gggttcacgt ccggcgaaac gtcgcccaac cggcggcgtc gagtcactgc
     1021 gcgctattcc gtggattttc gcctggacgc aaaaccgcct gatgctcccc gcctggctgg
     1081 gtgcaggtac ggcgctgcaa aaagtggtcg aagatggcaa acagaacgag ctggaagcca
     1141 tgtgccgcga ttggccattc ttctcgacgc gtctcggcat gctggagatg gtcttcgcca
     1201 aagcagacct gtggctggcg gaatactatg accaacgcct ggtagacaaa gcactgtggc
     1261 cgttaggtaa agagttacgc aatctgcaag aagaagacat caaagtggtg ctggcgattg
     1321 ccaacgattc tcacctgatg gccgatctgc cgtggattgc agagtctatt cagctacgga
     1381 atatttacac cgacccgctg aacgtattgc aggccgagtt gctgcaccgc tcccgccagg
     1441 cagaaaaaga aggccaggaa ccggatcctc gcgtcgagca ggcgttaatg gtcactattg
     1501 ccgggattgc ggcaggtatg cgtaataccg gctaatcttc ctctcctgca aaccctcgtg
     1561 cttttgcgcg agggttttct gaaatacttc tgttctaaca ccctcgtttt caatatattt
     1621 ctgtctgcat tttattcaaa atctggatat cccttcagat atccttaagg aattgtcgtt
     1681 acattcggcg atattttttt caagacaggt tcttactatg cattccacag aagtccaggc
     1741 taaacccctt tttagctgga aagccctggg ttgggcactg ctctactttt ggtttttctc
     1801 tactctgcta caggccatta tttacatcag tggttatagt ggcactaacg gcattcgcga
     1861 ctcgctgtta ttcagttcgc tgtggttgat cccggtattc ctctttccga agcggattaa
     1921 aattattgcc gcagtgatcg gcgtggtgct atgggcggcc tctctggcgg cgctgtgcta
     1981 ttacgtcatc tacggtcagg agttctcgca gagcgttctg tttgtgatgt tcgaaaccaa
     2041 caccaacgaa gccagcgagt atttaagcca gtatttcagc ctgaaaattg tgcttatcgc
     2101 gctggcctat acggcggtgg cagttctgct gtggacacgc ctgcgcccgg tctatattcc
     2161 aaagccgtgg cgttatgttg tctcttttgc cctgctttat ggcttgattc tgcatccgat
     2221 cgccatgaat acgtttatca agaacaagcc gtttgagaaa acgttggata acctggcatc
     2281 gcgtatggag cctgccgcac cgtggcaatt cctgaccggc tattatcagt atcgtcagca
     2341 actaaactcg ctaacaaagc tactgaatga aaataatgcc ttgccgccgc tggctaattt
     2401 caaagatgaa tcgggtaacg aaccgcgcac cttagtgctg gtaattggcg aatcgaccca
     2461 gcgtggacgc atgagtctgt acggttatcc gcgtgaaacc acgccggagc tggatgcgct
     2521 gcataaaacc gatccgaatc tgaccgtgtt taataacgtg gttacgtctc gtccgtacac
     2581 cattgaaatc ctgcaacagg cgctgacctt tgccaatgaa aagaacccgg atctgtatct
     2641 gacgcagccg tcgctgatga acnnnnnnnn nntataaaac cttctggatc accaaccagc
     2701 agacaatgac cgcccgcaat acgatgctga cggtattttc gcgccagacc gacaagcagt
     2761 actacatgaa ccagcaacgt acgcagagtg cgcgtgaata cgacaccaac gtgctgaagc
     2821 cgttccagga agtgctgaag gaccctgcgc cgaagaaact gatcatcgtt catctgctgg
     2881 gtacgcatat caagtacaag taccgttatc cggaagatca gggtaaattt gacggtaata
     2941 cagagcatgt tccaccggga ttaaacgcag aagagctgga gtcatataat gattatgaca
     3001 atgctaacct gtacaacgat cacgtggttg ccagcctgat taaagacttt aaagcgaccg
     3061 atccgaacgg tttcctggtt tatttctctg accacggtga agaggtttac gacacgccgc
     3121 cgcacaaaac tcaggggcgt aatgaagaca acccgacgcg tcatatgtac accattccgt
     3181 tcctgctgtg gacgtcagaa aaatggcaag cgactcatcc gcgtgatttc tcacaggatg
     3241 ttgatcgtaa atacagcctg gcggaactga tccacacctg gtcagatttg gcgggcttat
     3301 cttacgacgg ttacgatcca acccgttcag tggtgaatcc gcaattcaaa gaaactaccc
     3361 gctggattgg taacccgtat aagaaaaacg cactgatcga ttacgacact ctgccgtatg
     3421 gcgatcaggt gggtaatcag taattatcag ccagatgtta tttgccggat gcgacgcttg
     3481 acgcgtctta tccggcctac agatcgcggc ggtaggccgg ataaggcgtt tacgccgcat
     3541 ccggcaatac aagtcgactg aaactgccgc aatgtttaca tctcactccc atcaggccac
     3601 acttttcagg tggccttttt tcataatgcc agttactgtt ttcccgtcta catacctaag
     3661 ggaataacat gtatcacgac gtcagctatc tgctctcccg nnnnnnnnnn gagctggagc
     3721 agcgttagtt cccggcgatg tgctgtacgt ccctgctggt ggctggaatt ttccacaatg
     3781 gcaagccccc gccaccacct ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt
     3841 tgtgcaatgg gatggcaaac aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg
     3901 cccgcgcatt ggttcttttc tgctacaaac gctcaatgaa atgcaaatgc agtcgcagga
     3961 gcagcaaacg gccagactta tcgtcaccag cctgcttagc cactgccgcg atttgctcgg
     4021 cagccaaata cagaccgcct cacgcagcca ggctctattc gaagctattc gtgattatat
     4081 cgacgaacgc tatgcctccg cacttacccg cgaatctgtc gcacaggcgt tctatatttc
     4141 gccaaattac ctctcgcacc tgtttcaaaa aacgggggcc attggtttta acgaatacct
     4201 gaatcacacg cgactggagc acgctaagac attattgaaa ggctatgacc tgaaggtaaa
     4261 agaagtggcg cacgcctgcg gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa
     4321 aaataccgaa cgctcgccgt nnnnnnnnnn ccgtcggagt atcgtcggca gtaccacagc
     4381 caactgacag aaaaaccgac tactccagaa taagatgggt ttgctgcggc gcagaaagca
     4441 ctttgcgcac tgcgctcatc accctctgcg gctcacgcag gaaagcgtag atgctgcatt
     4501 gcacgtagcg acaatgttca aatcgctcag caccggcaag ctcgatatcc gtaattaaca
     4561 gcgcaacatc cgcccggcgg atatcctcgt ccgctaaacg attctccgtt cccaacgcgc
     4621 cctgagtttc aatgctgacg ccccacttct ctaactggca caacttttcc agccgttccg
     4681 ccgccatata agtatgcgcc acgccgctta cgcaggcggt tactgccacc agatatgcca
     4741 ttttaacctc ccacggtaac ctgaaatccg gcccgttctg ccatttcacg catcgttgcc
     4801 acatcggctg acgacggcgc agcaacctct ttcatcgacc atgttttccc cagcaggcgg
     4861 tatttcggtt ctccgtactg atgaaacggt aacagatgga tctgcctgat attcagcggg
     4921 atcagcacgt ccagcgcctg ctgcatattc tcccggctga gcgtgaaacc agggatcaac
     4981 ggtaaacgcg ggatcacgtt gacaccctca ctcaccagca aacgcagatt ctccagcacg
     5041 c
//
LOCUS       DAWDRC010000275         5006 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7334_length_5006_cov_2.691981, whole genome shotgun sequence.
ACCESSION   DAWDRC010000275 DAWDRC010000000
VERSION     DAWDRC010000275.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7334_length_5006_cov_2.691981"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    709..718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2622..2631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1444 a          986 c         1094 g         1462 t
ORIGIN      
        1 gacctacata ttttcatgaa taggtcgctc atttcagggt aaagcatatc ttttgtcatt
       61 accaacacaa taaatatggt ttttgatcgc ttttcccgat cgttttagca atcatgatag
      121 gtgcaatcta tcatgcgctg gttatcgatc ggtaaaaaca ttttgaaaat gattgttttg
      181 ttggggatga ttgctgaaca aatgatcaat accggaatta aacaggaatt cattttatga
      241 aaataaaagt tatagcattg gctacatttg tttctgctgt gtttgttggt tcagctatgg
      301 cctatgacgg aacaattacg tttacaggca aagttgtagc tcagacctgc aatgtgacca
      361 cagggaatga tggtaatttt acagtaacat tacccactgt atcgaataag gcccttgcta
      421 cccctggaga aacaaccgga ttaacaccat tttcaattaa cttaacaggt tgtacatcta
      481 ctgtagatgg agcaggtcac gttaaagcct tttttgagcc tggttccagt actgattttg
      541 tgacgaataa tcttaaaaat attgcttctg ataatgccgc tggtgatgta cagatccaac
      601 tattgaacag tgatggtact tctactattt tcttggggaa agattttgct ggtcaagatg
      661 tccattctga aactattggt ggggatggta atgccacatt acgttatann nnnnnnnngc
      721 tcaatattat gcgaccggga catcaactcc aggcaatgta aaaacaacag tcaattatac
      781 catcgcctac gaataacaaa cggttttgta ttttggggca ggaaatactg ccctgctttc
      841 ttctggagat accatgctaa ggtttgtgct gttttttttt actctatctg tatctgcagt
      901 tgttcagggt agtgtagtta tcatgggaac ccgtgttgtt tatccggcga ctcaaaagag
      961 tattagcata cgtttaaata atgacaatga atctccggca ttagtccagt cctggttaga
     1021 cgatggagat gcagcagctc cccctgagtc agtccacgtt ccttttatca ttactccgcc
     1081 aatatttcgt atggactcta aatcaggaca aacagtcaga attgtatata caggcgagtc
     1141 actaccgaaa gaccgagaat ctcttttcta tctaaatgtt ctggatattc cagcaaagcc
     1201 cagggcaaaa aaagactccg ataactcaga gcagcggaat aataattatt tgcaactagc
     1261 tgttcgtagt cgcataaaat ttttctttcg tcctgctcac ctgaagttaa caccaaatga
     1321 tgcttatctg aaagttacct ggcaccagga ggaaggccga attaaagcag ttaatccaac
     1381 accttattac ataacctaca acaaaattgc agttgaccag aataagcact taacgcctgt
     1441 tgaacatggt ggaatgattc cccctttctc ttcaacagta tttgcactta aaggcagaac
     1501 tgcgccagcc agcaaagttt cctgggttat tgttaatgat tacggaggat atcagcaagg
     1561 agaatctgtt ctggagtaag gtaataatga acgctaataa tctgtcatgc ctgatttact
     1621 gtcgttgttc tcttctgctt tttgctgcat tagggttaac agtaacaaac cattcatttg
     1681 ctgctgaaga ggctgagttt gattctgagt ttttgcattt ggataaaggg ataaatgcta
     1741 ttgatatccg ccgctttagt catggtaacc ctgtgcctga gggcaggtat tattctgata
     1801 tttatgttaa taatgtatgg aaggggaagg ctgatttgca gtatttacgt actgccaata
     1861 ccggtgctcc gacgttatgc ctgacgcctg agctgctttc attgattgat ttagtcaaag
     1921 atactatgtc gggaaacacc tcctgctttc cggcgtcaac agggctttct tcagccagaa
     1981 ttaattttga cttatcgact ttaaggttga atatcgaaat cccgcaggca ctgctgaata
     2041 cacgtccaag aggatatatt tccccttctc agtggcaaag tggtgttcct gcagcattta
     2101 taaactatga tgctaactat taccagtata gctcttccgg gacgagtaac gaacagactt
     2161 atctgggatt aaaagctgga ttcaatttgt ggggatgggc tttgcgccac cgtggcagtg
     2221 agagctggaa taatagctat cctgccggat atcagaatat agaaacaagt ataatgcatg
     2281 accttgcccc attgagagca caattcacat taggggattt ttatacgaat ggtgagctaa
     2341 tggatagcct cagtttgcgg ggagtcaggt tagcatcgga tgaacgaatg ttacccggtt
     2401 ctttacgtgg ctatgctcct gctgtccggg gaatagctaa cagtaatgct aaagtaacca
     2461 tttatcaaaa tgctcatatc ctctatgaaa cgacggtgcc agccggacca tttgtcatca
     2521 atgatttata tcccagtgga tatgctggtg accttctcgt taagataaca gagtctaatg
     2581 gccagacacg aatgttcacg gttccttttg cggccgttgc tnnnnnnnnn nttagtcgct
     2641 ggcaaatgtc agtgggaaag tatcgttatg cgaataaaac atataatgat ttaatagcac
     2701 aaggcaccta tcaatacggc ctgacgaatg atattacttt aaacagtggt cttaccacag
     2761 cttcaggata tacagcgggg ttagctggcc tggcctttaa tacccatctg ggtgctatag
     2821 catctgacat tacattgtcc agaacagcat tcaggtattc cggtgtaacg cgtaaaggtt
     2881 atagtctgca ctcaagttat agcatcaata ttccagcctc aaacacaaat ataactctgg
     2941 cggcttatcg ttattcatca aaagattttt atcatctgaa ggatgcgcta tcagctaatc
     3001 acaacgcgtt tattgatgat gtttctgtaa aaagtacagc gttttatcgt cccaggaatc
     3061 aattccagat ttcaatcaac caggaattag gtgaaaaatg gggtgggatg tatttaacag
     3121 gaacaaccta taattactgg ggacataaag gaagtcgtaa tgaataccag atggggtaca
     3181 gcaacttctg gaaacaactc ggctatcaaa ttggattgtc tcagtcaaga gataatgagc
     3241 aacaacgccg tgatgacaga ttttatatta attttactct ccctctggga ggaagtgttc
     3301 aaagcccggt gttttccact gttttaaatt atagcaaaga agagaaaaat agtattcaga
     3361 catcaattag tggtactggc ggggaggata atcagttctc ttatggtctt tcaggaaaca
     3421 gccaggaaaa cgggccttcc ggttatgcaa tgaatggggg ttatcgttca ccttatgtaa
     3481 atataaccac aacagtcggg catgatactc agaataataa tcaaaggtca tttggtgcgt
     3541 cgggagcggt ggtcgcacac ccctatggag tgacattgag taatgacctg agtgatactt
     3601 ttgccattat ccatgctgaa ggagctcagg gggctgccat caataatgcc tctggtagtc
     3661 gtctggattt ttggggaaat ggtattgttc cttatgttac accctatgag aaaaatcaaa
     3721 ttagcatcga tccctccaat ttagatttga atgttgaatt atcggcgacg gagcaggaaa
     3781 tcattcctcg tgctaatagc gccacgttag tgaaatttga cactaaaaca ggaagaagtc
     3841 tgttatttga tattcgtatg tctactggca atccccctcc aatggcttct gaagttctgg
     3901 atgaacatgg acagttggcc ggatatgtcg ctcaggccgg gaaggtattt accaggggac
     3961 tccctgaaaa aggtcatctc agcgttgtat ggggaccaga taataaagac agatgttcat
     4021 ttgtatatca tgttgcacac aataaagatg atatgcaatc tcagctcgtt cctgttctgt
     4081 gtatacagca ccctaatcag gaaaaaacat gaaaagaatc tttttcatac cattgttttt
     4141 aattttactc cctaagctgg cggtagcggg tccggatgat tatgtgcctt cgcagatagc
     4201 ggttaataca tccacattgc caggtgttgt gattggtcct gctgatgccc atacctatcc
     4261 ccgggtgata ggagagctgg cgggaacaag taaccagtat gtttttaatg gcggtgccat
     4321 cgctctgatg cgtggaaagt ttacacccgc actgcctaaa attggaagta ttacgtatac
     4381 tttccatcaa ggaaacagcc gtgattcatc tgattttgat atctatgata ttggtgtatc
     4441 cggactgggt attattattg gcatggcggg ctattggccc gcaacgcctc tggtccccat
     4501 aaatagctca ggtatatata ttgaccctgt aggtgccaat acaaacccca atacttataa
     4561 cggtgcgaca gcaagcttcg gagctcgttt gtttgttgct tttgtcgcaa cgggaagatt
     4621 acccaatgga tatataacaa tacccaccag gcagcttggt actattttgt tggaagcaaa
     4681 acgtacaagt ttaaataata aaggactgac agcacctgtt atgttaaatg gtgggcgcat
     4741 tcaggtacag agtcagacat gtaccatggg gcaaaaaaac tatgtggtgc cattaaatac
     4801 cgtatatcaa tcacagttca catctttgta taaagaaata cagggaggta aaattgacat
     4861 acacctacaa tgcccggatg gaattgatgt ttatgctaca ttgacagatg catcacagcc
     4921 agtgaacaga acagatatat tgaccttaag cagtgaatcc actgcaaaag gatttggcat
     4981 caggctatat caagacagtg atgtaa
//
LOCUS       DAWDRC010000276         4997 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7352_length_4997_cov_2.070619, whole genome shotgun sequence.
ACCESSION   DAWDRC010000276 DAWDRC010000000
VERSION     DAWDRC010000276.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7352_length_4997_cov_2.070619"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    457..466
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4736..4745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1235 a         1284 c         1284 g         1174 t
ORIGIN      
        1 acccgcagat tgttaacggt gcgttgggca agttaggcgc ggcgcggcca ggattgcgtt
       61 tacctggttg tgttgatgct tttgagcagg gcgtgcgggc gattttaggc caactggtga
      121 gcgtggcgat ggcggcaaaa ttgacctcca gagtggcaca gctttatggc gaacggctgg
      181 atgattttcc ggattatgtc tgcttcccca cgcctcagcg gctggtggta gccgacccgc
      241 aggcattaaa agcgttaggt atgccgttga aacgggcaga ggcgctgatt catctggcaa
      301 atgcggcgct ggagggcacc ttaccaatga caataccggg cgacgtagaa caagcgatga
      361 aaacgctgca aacctttccg ggtatcgggc gctggacggc aaattatttt gctttgcgtg
      421 gctggcaggc gaaagatgtt tttctgccgg atgattnnnn nnnnnnttat ctgattaaac
      481 agcgatttcc gggaatgacg ccggcacaaa tccgccgtta tgccgaacgc tggaagccct
      541 ggcgttctta tgcgctgttg catatctggt atacggaagg ctggcaacca gacgaagcat
      601 gacgttggtt gccggatgcg gtgcgagcgc cttatccggc ctggggaatg agcgatttgt
      661 aggcatgata agacgcgtta gcgtcgcatc aggcaatggg caaacgctcc cttagttaat
      721 cgcgaaataa cttctattca ccagcaaatc cagcggttgg gcattggcaa tcacatcacc
      781 ataaatcaga tcgacgccga tgcccgaaag cgtatccatc actaagggta aaacgactgg
      841 cccggcgatg gttttcatcc cgagtcgctg agcgtgcccc tgaataatcg taatcagcat
      901 ctcatccatc aaattaccct gcacgttggc gcataactca ccatcaagta gcaggtaatc
      961 tgccatattt gctttcagcg agttgaagat ttgcagatcg cggcccacct gactgaatac
     1021 gatccgacat cccgccagcc gcagtttttg cacgctttct gcgtgatcga aaatcgcttc
     1081 tgccggaata atcagatgta ataaccgtgc tggtagaggg ctattttcca gctgctcaag
     1141 cagttcattc accagcgtgg cgctactcaa accagcaacg gaaaggggga gggcgatgct
     1201 taagccttta ctggcaaccg cttttgcggc ctgctggaaa aaatcgtgga atacccgtcg
     1261 gtcaagagca tggctaagtg ccggatcgct gaagctacga cgaaatgttt gttcatcaat
     1321 aatctcgctt tcgcaactcc agagcttaag tgaaatcagc cacaaattac ttgcttccgg
     1381 gagccgtggc gaagcgatgc cgtcggcgat catcatcaac tgattctctt taatcatccg
     1441 ccactgttca tcaagcgata tcgctgcccg ttcgctatgt gcggcagctt gctgcggttc
     1501 gtaaatcgtc acccggcccc ggccaccatt tttggaggca taacaggcga tatcagcctg
     1561 cgacatcact tcagctgcct gatgattgtt gtcatcaatc aaggtaatcc cggcactggc
     1621 acctacccga tgcacacgtc cttcccatat aaagtgatag tcattcacgg cactgataat
     1681 gcgtgtagcg ataaaacgag cgctttcaac attgcagtct ggcagtagca gaccaaattc
     1741 atcaccaccg agccgcgcca gcacgtcgct ggagcgcagc atactcagca tcaacgacgc
     1801 cagttcgcgc agtaaagcgt cgcccgccgc atgcccggcg ctgtcattca ccgctttaaa
     1861 gcgatcaaga tcgataaaca ccagggcatg tcgctgatgc gtactgttta ccgtttgcag
     1921 caggatacgc agttgcttct caaaactggc gcgattggcg agatgcgtca gtgcatcatg
     1981 ggaggcgctg tagctcagct ggcgcagcat tttgcgtgat tcggtaacgt cctgaatcac
     2041 cagtaccgag cctatattgc tgccgtccag agtacttaac ggcgtaatac tgtaatgcac
     2101 gtcgtagctg ccgccgctcc ggcagtgcaa taccacatct tgttcgatcg cggaacgtga
     2161 ggtgtcagca ctgtaaatgt tctccattaa tggtccgttg tcgccaaaag taatatgcaa
     2221 caccgtcagg agcggaacac ctaacgcttc ttcctgcgtc cagccgctca ttttctccgc
     2281 cactggattc ataaaggtaa ttttcattgc cctatcaata cagaccacgg cttcgccgat
     2341 ggagtccagc gtaatgtgca ggcgctcttt ttcctgaaac aatgcctcgt taagctgttt
     2401 aacctccgtc atatccatat taatgccgag caggcgttcg acctcgcctt ctttattcag
     2461 tacccggttg gcgagggcgc ggatatggcg gatgccatct ttcacggcaa tgcgaaattc
     2521 cagtttaaag ggcgagcgtg attgcaacga atcacgaatc actttttcgg cgtgctggcg
     2581 atcttccggc agcacgcact cgtaccacac ctgccagttc ggtttgatat gcggaggaat
     2641 ttcatacagc tcgaacatcc gcttatccca actaaaaata ttcggcttca gctcccactc
     2701 ccagatgcca atcccgcccg cttcgttggc cagcgtgatg cgctccatca gttgctgatt
     2761 gacctgttcg gtgcgtttta gctcgttaat gtcttcaatc tgagcgataa aatagagcgg
     2821 cgtgccatcc gtgtggcgca ccagtgagac ggcaagcaac gcccagacaa catcgccatt
     2881 gcggttgtag taacgttttt ccattgaata ggtgtttatt tcaccgctta tcagcttttc
     2941 aacctgttgg agatctttat tgagatcctc cggccaggtc agttgctgaa aggtgagtcc
     3001 gcgcagctct tcctgactgt aaccgagaaa ctggcagagc gctttgttgg attgcagcca
     3061 ttgtccctcg gtgcccacta acgccatgcc gatagcggaa tattccatcg cgttccgaaa
     3121 gcgggtttcg ctttcggaaa tgtgtttgcg ttccgcacga aaggcataca tcaccatcgt
     3181 catgatgttg gcgggcagca ggatcagcaa aaacggtagc cacggcatat ggctcatcag
     3241 gtacatacgc ggcgtagcaa gcagggaggg atccgcggcc atcatcagcg acaccatcat
     3301 caccgtggta aggaagatca aaaaggcttc cattcgtggc aggcgcacgg cgctccacat
     3361 caacagcaca ataatgaaag taaaaggcca cggcagatac agcatcgaaa gccagcttaa
     3421 cgtcagcgtg atggctaacg tgagcagcga ctcaaaaagc aaccttgggt tgcgatggcg
     3481 cagcagatag tgtggtttaa ataacaatcc cagcggcacc agtgccagag cgccgatgga
     3541 ttctgacagt acccatatca aaaatgccct gagaggatcg tctccgggcg tcagcaggac
     3601 aaccagaaca ccccccaaca gaggcggaat aatggcgctg ccgagtgcca gacgcagcca
     3661 gtcagccaga ttttgcaagg ggttatacca cggcagcaat ttacgtagca gcactgcccc
     3721 gaccacggct tcaacaatat tgatggtcgt ccaggtcatg ttcagcgagc tggtggaaaa
     3781 aagcaggatg gatgcggcga tatttcccag cgaacagctc agcgcaattc ccggccacat
     3841 gcggccggca tggcgataaa acgccaccat catgatggac gtcgggaacc acagtggggc
     3901 gagttgggtg ccaaactgcg aaagctcaag cgagaaaagg gtaaagataa acgagactaa
     3961 acctaaactg accaggtgaa gcagcgggtg gggcagggca attaatacat gctgtgattg
     4021 tttgctcatt accgctttat ccgcaagacc aggtcgccag tattcgtttc tgtgtttatg
     4081 ctagtacaaa agttttacat tttatatgcg aattgctcat aaagtgacgt aaaggcggat
     4141 aatttgcgca actgcgtata acaattttta ccttgtataa aactgatcaa cgtaatttgc
     4201 caccgggaat tcttcgtcaa caattaactc tgagcgcgcg gcaacgctat tcgactggta
     4261 tcaggcggat gaaatcccta taattgccgc gtttggcgct tcgtcgcccc cttcctaaca
     4321 tccaggttaa tcaggtcgct aaatatatga ctgatcagtc tcatcagtgc gtcattatcg
     4381 gtatcgctgg cgcatcggct tccggcaaga gtcttattgc cagtaccctt tatcgtgaat
     4441 tacgtgagca agtcggtgat gaacacatcg gcgtaattcc cgaagactgc tattacaaag
     4501 atcaaagcca tctgtcgatg gaagaacgcg ttaagaccaa ctacgaccat cccagcgcga
     4561 tggatcacag cctgctgctt gagcatttac aagcattgaa acgcggctcg gcaattgacc
     4621 tgccggttta cagctatgtt gaacatacgc gtatgaaaga aacggtgacg gttgagccga
     4681 agaaagtcat cattctcgaa ggcattttgc tgctgacgga tgcgcgtttg cgtgannnnn
     4741 nnnnncccga tgtttctgca attcattgag ccttctaaac aatatgcgga cattatcgtg
     4801 ccgcgcggcg ggaaaaaccg catcgcgatc gatatattga aagcgaaaat aagtcagttc
     4861 tttgaataag cttgataaat tgtgtaccgt tcagtgataa cctggtatgc ccttgacgta
     4921 attggcgtta agggagtgat gcacgaaagg agaaaatgcc atgcgtctgt gtgaccgaga
     4981 tattgaagcc tggcttg
//
LOCUS       DAWDRC010000277         4989 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7367_length_4989_cov_2.332793, whole genome shotgun sequence.
ACCESSION   DAWDRC010000277 DAWDRC010000000
VERSION     DAWDRC010000277.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7367_length_4989_cov_2.332793"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1668..1677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2501..2510
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1085 a         1291 c         1360 g         1233 t
ORIGIN      
        1 tttcaatcgc ttttaatctc cggggtttgc aggctgctca acagctctgc aaaccctgcg
       61 ccttccgcta ccagacgctc tttttgccgt ttggtcagtt gcttaacgcg atattccgcc
      121 cgtagtgcca gcgaacgatc gcccactggc gcggaaaacg ccagcgttag ttctcctttc
      181 ccgcgcagtg ctttcgcccc tttgccgctt tggtgctgct gatagcggcg ttcgacatct
      241 gtggtgatcc cggtataaag cttattgtcg gcggtacgga tcaagtaaag aaaccagggt
      301 gtcatcgtct gcgtcgcatg ttaaggtcag gcccacagta taaaagagaa cagcaatgga
      361 aacactcacc gccatcagcc gttggctggc aaaacaacat gttgtcacct ggtgtgtgca
      421 gcaggaaggg gaactgtggt gcgccaatgc tttttatctt tttgatgcgc agaaagtcgc
      481 cttctacatt ttgacggaag aaaaaacgcg tcatgcgcag atgagcgggc cgcaggcggc
      541 aattgccgga acggtaaacg gtcagccgaa aacggtagcg ttaattcgcg gcgtgcagtt
      601 taaaggtgag atccgcagac tggaaggcga ggaaagcgac ctcgcacgca aggcgtacaa
      661 tcgtcgcttt ccggttgcca gaatgctgtc ggccccggta tgggaaatcc ggctcgatga
      721 aatcaaattc accgacaaca cgctgggctt tggtaaaaaa atgatttggc tacgtaactc
      781 aggcaccgag caggcgtaac gcctcgcggt taaacgcggg cagatcgtcc ggtgtccggc
      841 tggtaaccaa ctgatcttta tcgaccacca cttcctgatc gtaaaattcc gcgcccgcgt
      901 ttttaacatc aatcacgatc ggtttaacgg cggtcagctt acgaccacgg atcacatcgg
      961 cgctgatcag caactgggga ccgtgacaga tggcgaaaac aggtttgccg ctgttcacaa
     1021 aatcgcgggt aaaggtgaca aaacggttgt cgccacgcag ataatctggt gaatgaccgc
     1081 ccggtagcag cagggcatcg aactctgcgg gcgtcacttc atcgatggat ttatcgatgg
     1141 ccacgctggc ttctcctttt ttgcctttca ccgttttacc cgcttgtttt tcaatggtaa
     1201 tcacttcgtg tccggcttta cggaactcgt ctgcgggtga agtaaattct gaatcctcaa
     1261 attcatcagt gattaaaacg gcaattttct tactcatgct tcctccgcgt tgttgcattg
     1321 gatgaggcga attttccaca atcggggata attacctcat ggtctattaa gcctggtgca
     1381 tgagccgggg agcgcaaggc agacaattct gtaaaggagc gaagatgagt caggtactga
     1441 ttacaggcgc aacgggactg gtgggcggtc acctgctgcg gatgttgatt aatgaaccga
     1501 aagttaacgc catagccgcg ccgacgcgac gcccgttggg tgatacgccc ggggtgttta
     1561 atccccatga tccgcaactg accgacgcgc tggcgcaggt caccgatccc atcgacattg
     1621 tgttttgttg tctcggcacc acgcggcgag aagcggggag caaagagnnn nnnnnnngat
     1681 tacacgctgg tggtggatac cgcattaacc gggcggcggc tcggtgcgca gcatatgctg
     1741 gtggtcagtg ctatgggtgc taatgcccac tcgccgtttt tctataaccg tgtcaaaggg
     1801 gagatggaag aagcattaat cgcccagaac tggccgaaat tgaccattgc tcgcccgtcg
     1861 atgttactgg gcgatcgtag caaacagcgg atgaacgaaa cgctttttgc gccgctgttc
     1921 cgtttgttac ccggtaactg gaaatccatt gatgcgagag acgtggcgcg ggtcatgctg
     1981 gcagaggcga tgcggcctga gcatgaaggt gtgacgattt taagctcttc agaattacgc
     2041 aaaagagctg aataattatt cccaagaatt gatagtgagc gtaatattca cgcgctcaat
     2101 tatcataact tattcctggg gaatgctatg actggtcagt cttcatctca ggcggcaacg
     2161 cccattcagt ggtggaaacc cgcgcttttc tttctcgttg tcattgccgg tctctggtat
     2221 gtgaaatggc aaccttacta cggcaaagcg tttactgctg ccgaaaccca cagtatcggt
     2281 aaatctatcc ttgcgcaggc agatgctaat ccgtggcagg cggcgttgga ttacgcgatg
     2341 atctattttc tcgcggtatg gaaagcggcg gtgctggggg tgatcctcgg ttcgttgatt
     2401 caggtgctga tcccgcgtga ctggttgttg cgtacgcttg ggcaatcgcg ctttcgcggc
     2461 acgttgctgg gaacgctgtt ttcgctgccg ggcatgatgt nnnnnnnnnn gggttcgtcc
     2521 tcggctgggg ttttgcggcg attcgtctgg tggcggggct ggtgatggtg ttgctgattg
     2581 cgacgctggt gcaaaaatgg gtgcgtgaaa caccgcaaac gcaggcaccg gtcgaaattg
     2641 acatactgga atcacagggc ggttttttta gccgctgggg cagggcgcta tggacgcttt
     2701 tctggagtac gatcccggtt tacatccttg cagtactggt gttgggtgcc gctcgcgtct
     2761 ggttattccc ccatgccgat ggtgctgtcg ataacagcct gatgtgggtg gtggcgatgg
     2821 cggtagcggg atgcttgttt gtcattccca cggcagcaga aattccgatt gtacaaacga
     2881 tgatgctggc aggtatggga accgctccgg cgctggcatt gttgatgacg ctcccggcgg
     2941 tgagtttgcc gtcgctgatt atgctgcgca aagcgttccc gacgaaagcc ttatggctga
     3001 cgggggcgat ggtggcagtg tctggtgtga ttgtcggcgg gctggcgctg ttggtttgat
     3061 ttgagggaaa acatgtcgga tgcggcgtaa acgccttatc cgacctacgg tttacatcgt
     3121 tgtaggcctg ataagacgct gcaagcgtcg catcaggcat tacaagggct gctatttaat
     3181 aaacgtaaac gccgtagtta cccgcttcac gccgcttacc cggctggcaa tatctgccgc
     3241 cgctttcgct tcacgttcag tcaccagccc catcaggaac acttcaccgt tttcggtggt
     3301 cactttcacg ttggacgatt tcaccaggtc gctggttaac agctgcgaac gtactttggt
     3361 ggtgatccac gtatcgttag acgcttcgcc cagaccaatc ggctggccct gacgaatctc
     3421 gttatacact tcgttggcac cgtctacgcc catagcaatc tgtttggcgc gagccgaaag
     3481 ttcagcgttt ggtgactgcc caaccagcag cactttgccc tgataggccg ttacattaat
     3541 gcgcgcttct ttcttaattt gttcgtcttt cgacaatgcg ctgttcacgc gcacttccag
     3601 ggtaccatcg tctacctggg tgccgacact gcgtgggtca gttgcggctt tggtacccac
     3661 agcagcagta cccactacgg cagcggcaac acaaccttgc aacagcagcg cggaaataag
     3721 gactgcgatt ggcgataatg ccttcatgta ttctccttaa acatcctggt gagggaaaag
     3781 cgtgttatcg atcagatcgc acaggcaatt taccgtcagc atatgcattt cctgaatgcg
     3841 agcactacga tgcgaaggaa tgcggatctc cacatcctgt ggccctaaca aacctgcaag
     3901 ttcaccgccg tcatagccgg ttaatgccac aatggtcata tcacgcgtaa cggcagcttc
     3961 taccgctttt acaatatcgc ggctgttgcc acgggtggaa atggctaaca atacatctcc
     4021 cgcatggccc agcgcccgca cctgttttgc atacacttca tcatgtaagc gatcgttggc
     4081 aattgccgtt aagacaacat tatcagtatt tagtgcaatg gcaggtaagc tgggccgctc
     4141 cgtttcgaaa cggttgatca tgctggcagc aaaatgctgt gcattggcag cggaagttcc
     4201 attaccacaa cagaggattt tgttgccatt gagcagagac tgaaccagcg tcatggctgc
     4261 acgggagatg gcatccggaa gcgcctctgc cgccgcaatt tgagtttgaa tgctttcagt
     4321 gaagcaagct ttaattcttt cttgcacgct aatccttaaa ccttaattat gagtggtcat
     4381 taaaggcatc cttaatccac tcaacctcat tcccggtgaa ggctaccaca tcgaaccggc
     4441 aatccacagt atcaaaactc ccattatgac gcgcgagcca caagcgggca gtctgtaata
     4501 atttgtgttg tttgctgcgg gtcacactgg ctgccgcgcc gccataaagc gcagagcggc
     4561 ggtaacgcac ctcgataaaa acggtggtcc ggccttcacg cattatcaga tcgatctcgc
     4621 cgccacgctc gttcacgtta gcggcgataa accgtagtcc tttgccttcc agccagcgac
     4681 gcgcttgtgc ttcccacgca tcgccggtct gtttggtggt taactggcgg ggactacctg
     4741 accttgttgg tactgtagcc atgataactt cctgttaatc acgcaatccg ggtttgccgt
     4801 caggcttccg gtattaccgt tgatttcaaa accctgaacc tggcgcattt gtgagaaatg
     4861 atttgccaac gaccaggcat cgacgcccat cgcatacatg cgagccagcg aataatcgtt
     4921 gttcaccgcg ctgagcgcct gctgcattaa cggcagatta ccgcctgcca gcatcgggat
     4981 ttcgctgta
//
LOCUS       DAWDRC010000278         4985 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7374_length_4985_cov_2.625558, whole genome shotgun sequence.
ACCESSION   DAWDRC010000278 DAWDRC010000000
VERSION     DAWDRC010000278.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7374_length_4985_cov_2.625558"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3339..3348
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1242 a         1308 c         1314 g         1111 t
ORIGIN      
        1 cggcgtgttc agcgcgctgg atttacctgc gttggaacgg cctgcaaaag ccacttcaat
       61 tccggtatcg gaaggtaggt ggcgaatatc aggcgcactc atcacaaaat gcgtctgttg
      121 ataattcaaa ttagtcaaag cggtcgtctc catcagtcaa agctttgcgg cgattatacc
      181 tgaaccataa taaaaggcgg atttttcggc gtgagcgttg taagtaaaag ccatacgctt
      241 tgtgagacat tgccgatagt ctttatgcga aatagcagag aaaattctgc gacgcatgtc
      301 aaataagcta tataaatcag tgaattgctt ttatataaaa ctctgaaaat gtgcaaatac
      361 tgatggcggt tgattgttta tttaaagcaa aggcgtaaag tagcacccat agagcgagga
      421 cgctaacagg aacaatgact caggatgagg gtcaggagcg ccaggaggcg aagacagagg
      481 attgtcagga agataaacgt ccggagacgt aattaaatgg aaatggaatc aacacggatt
      541 gttccggcta atggaaaaac agggtgtgtt ggcggcctgc aaggattgta agacccgtta
      601 agggttatga gtcaggaaaa aaggcgacag agtaatctgt cgcctttttt ctttgcttgc
      661 tttctgttag attccgccgc aaatctatac tgaataaaac ggctaaaaga cgaaccatta
      721 tgaaaccatc atcttcaaac tcacgcagca aaggtcatgc aaaagcgcgt agaaaaacac
      781 gcgaggagct ggatcaggaa gctcgtgacc gtaagcgtca gaaaaaacgt cgtggtcatg
      841 cgccgggcag ccgcgcagcg ggtggtaaca ccacgtcagg cagcaaaggc cagaacgcac
      901 caaaagatcc acgtattggc agtaaaaccc ctattccatt gggcgtggct gaaaaagtca
      961 ccaaacagca caaaccgaag agtgagaaac ctatgctttc accgcaggcg gagttggagt
     1021 tactggaaac ggatgagcgt ctggatgcgc tgctggaacg tctggaagca ggcgaaaccc
     1081 tgagcgccga agagcaatcc tgggtggatg ccaaactgga tcgtattgat gagttgatgc
     1141 agaaactcgg cctctcttat gacgatgacg aagaagagga agaagacgag aagcaagaag
     1201 acatgatgcg tctgctgcgg ggcaactaac ggattgccgc cgtgggcctt cccgttctgc
     1261 tgataaccct tccggttata tgttatctgg tgtggttatt cgttaaacta cagcggttgt
     1321 cgcggcgaca aaagtggctg cgcaaccggc tgatgactcg aaacgggcat ccgccggtac
     1381 gccgtagccg ccagagacgc catcggaagg agtgagcatg tctgtacagc aaatcgactg
     1441 ggatctggcc ctgatccaga aatataacta ttccgggcca cgatacacct cgtacccgac
     1501 cgcgctggag ttttcagaag acttcggcga acaggcgttt ttacaagccg tggcgcgcta
     1561 tcctgagcgt ccattatctc tctacgtaca tattccgttc tgccataagc tttgttactt
     1621 ctgcggttgc aataagatag ttactcgcca gcagcacaag gccgatcagt atctggacgc
     1681 gctggagcaa gaaatcgtcc atcgtgcacc gctttttgcc gggcgcaagg tgagccagct
     1741 gcactggggc ggtggtacgc cgacgtatct gaacaaagcg caaatcagcc gtctgatgaa
     1801 gctgctgcgc gaaaacttcc agttcaatgc cgatgcggag atctcgatcg aagtcgatcc
     1861 gcgggaaatc gaactggatg tactcgatca tttacgcgcc gaaggcttta atcggctgag
     1921 catgggcgtg caggacttca acaaagaagt gcaacgtctg gttaaccgcg agcaggatga
     1981 agagttcatc tttgcactgc ttaaccatgc gcgtgagatt ggctttacct ccaccaacat
     2041 cgacctgatt tacggcctgc cgaaacagac gccggagagt ttcgccttta ccctgaaacg
     2101 cgtggcggag ctgaaccccg atcgtctgag cgtctttaac tacgcgcatt tgccgaccat
     2161 ttttgctgct cagcgcaaaa tcaaagacgc tgacctgccg agtccgcagc aaaaactcga
     2221 tatcctgcag gaaaccatcg ccttcctgac acaatcgggc tatcagttta tcggtatgga
     2281 tcactttgct cgcccggatg acgagctggc ggtggcccag cgtgaaggcg tgctgcatcg
     2341 taacttccag ggctacacca cccagggcga taccgatctg cttgggatgg gcgtttccgc
     2401 catcagcatg attggcgact gctacgcgca gaaccagaaa gagttgaagc agtactatca
     2461 gcaagtggat gaacaaggca atgcgctgtg gcgtggtatt gcgctaacgc gtgatgactg
     2521 tattcgccgc gatgtgatta agtcgctcat ctgcaacttc cgtctggatt acgcccctat
     2581 tgagcaacag tgggatttgc tcttcgctga ttactttgcg gaagatctca agctgctcgc
     2641 cccgttagca aaagatgggc tggtggatgt ggatgagaag ggaatacagg tgacggcgaa
     2701 aggtcgcttg ctgatccgca acatttgcat gtgctttgat acctatctgc gccagaaagc
     2761 gcggatgcag cagttctctc gggtgattta aattgtgaat ggcgcttcgt ttacgaagta
     2821 gaaaacgaaa gcgccatcaa tgtgctgaga acaagattgc ctgatgcgct acgcttatca
     2881 ggcctgcaaa atctgttgaa attattgctt ttgtaggccg gataaggcgt ttacgccgca
     2941 tccggcaatc aacgcctgat gcgacgctgg ttgcgtctta tcaggcctac gactttctcc
     3001 gcatccggcg taaacaacga gcttcagcta aacagcccta tcatcgcggc acacagcacg
     3061 gcagcaacag cagtttgcgg tgtgtggctg tcaactgcgc cactcgatac cagattaatt
     3121 attgattcca gcataatgac tctccccgtt ttccgggcaa gatcatactg aacttatcgg
     3181 aacagtaaag cgcaaaatac cagcaatttg cgctcaataa tcaatcttta cacacaagcc
     3241 gtgaatcact ccatccccag ctcttttaac ttacgcgtca gggtgttgcg accccagcca
     3301 agtagccgcg ccgcttcctg tttatgcccc tgcgtatgnn nnnnnnnncg cggtggtcag
     3361 taacgtccgt tccagctctg gctgtgcttc ggaaagcagg ttttgatgac cggaacgcag
     3421 cgctctgtct gcccactgtg ctaacagcgt tgcccaactg tccggctgca tgtgtgaagt
     3481 gctttccggt acgtttgatt caaacagttc gccaggcaaa tcctgaatca acacttcctg
     3541 cccggcggcc atcaccgtta gccagcgaca ggtgttttcc agctggcgca cgttgcctgg
     3601 ccacgccaga cgcgtcagcg cggcttcggt ttccggatgc agcaacttcg cttctacgcc
     3661 cagctctcgc gcggcaacct gtaaaaaatg gcgtgccaga cggggaatat cttcccgacg
     3721 ttcgcgcagt ggcggcagat gaacgcggat gacgttcagg cggtggaaca gatcctcacg
     3781 gaacttgcct tcctgcaccc gctgttcaag attctggtga gtggcagcga taatccgcac
     3841 atctactttc accggcgcat agccgccaac gcgataaaac tgaccgtctg ccagcacgcg
     3901 cagcaaacgc gtctgcacat ccagcggcat atcgccaatt tcatcgagaa ataatgtacc
     3961 gccatcagcc tgttcaaaac gcccctgacg aatggtattc gcgccggtaa atgcgccttt
     4021 ctcgtgacca aacagttcgg attcgatcaa atccttcggg atagccgcca tattgagcgc
     4081 gataaacggc gctttggctc gcggactgtg gcgatgcagg gcatgagcga ccagttcttt
     4141 accggtgccg gattcgccgt taatcagcac gctaatagaa gaacgcgaaa gccgaccgat
     4201 aatccggaac acgtcctgca tggctggcgc ttcaccgatg atatcggtcg ttgggccgtt
     4261 aagctgaaca ttacgcggct gctgctgttc ctggtaatga ctgatggcgc gctcaaccag
     4321 cgccacggct tcgtcgatat caaacggttt gggcagataa tcaaatgccc cttgttgata
     4381 ggcgctgacg gcagcatcca gatcggaatg tgcggtcata atgatgaccg gaagcatcgg
     4441 atggcgctgt ttaatctgct tgagcagcgc cagcccgtcc attcccggca tacggatatc
     4501 tgaaagcagc acatccggcg ttttgctcgc cagcgcctcc agtacttccg cgccgttctc
     4561 aaatgtcgta caggttaaac ccgctccagc gagcgcacgt tcaagcaccc aacggatgga
     4621 actatcgtca tcgactaccc agactatccc tcgttgcata aacgtcacct ttatttcctg
     4681 ataggcaggt aaaccgagaa ctcggtatga cctggccaac tggtaaattc aattttgcct
     4741 gaatgctgat caatcaaatt acgggcgatg gataagccaa gcccggtgcc accttcgcgg
     4801 ccgctgacca tcgggtaaaa cagcgtatcc tgcaaatggg gcggaatgcc cggcccgtta
     4861 tcttccacat caatccgcgc cgccagccga tagcgctcgc cgtgtaaggt cagttgaaac
     4921 gctgtgcggg tacgtagaat gatttcacca ccctccggcc ccagcgcctg tagcgcattg
     4981 cgcac
//
LOCUS       DAWDRC010000279         4970 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7397_length_4970_cov_1.758901, whole genome shotgun sequence.
ACCESSION   DAWDRC010000279 DAWDRC010000000
VERSION     DAWDRC010000279.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7397_length_4970_cov_1.758901"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2657..2666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4416..4425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4652..4661
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1254 a         1187 c         1321 g         1178 t
ORIGIN      
        1 ttttacagat tagtaatcaa tccctatctg cgcttttaca ccggcatcaa acgcatgttt
       61 gacggggcgt aattcactta ccgtatccgc cagttcaaga atatcccgat gacaaccacg
      121 acctgtgata atcaccgtct gttgatgtgg acgttcattt aacgcctgca ccacttcttc
      181 cagtggcaaa tagtcatacg ccaccatata cgtcagttca tcgagcaaaa ccatatccag
      241 tgaaggatcg gccagcattc gtttggcatg ttgccagact tcacggcagg ccgcggtatc
      301 agattcgcga ttttgtgtat cccaggtaaa gcccgttgcc atcacctgaa actcaacgcc
      361 atgtggctcc agtagattgc gctcgccatt aggccaggtg cctttaataa actgcacgat
      421 acccactttt ttgccatggc ctactgcacg agttgccgta ccaaatgccg cggtggtttt
      481 gccttttcca ttgccggtaa agacgatgat aataccgcgt tcatcctggg ccttggccac
      541 acgagcatcg actttttctt tcactcgctg ctgacgctgt tggtagcgtt catcactcat
      601 tgggaaattc ctggtttacg gcccggttgg gcgtcaaagg tcatgccggt tttacggcgg
      661 ctgtcatcgc ccatcagcca gaggtagagc ggcatgatat cagcgggtgt tttaagtttc
      721 tgtggatctt cggtcgggaa ggcgctggca cgcattgcgg tgcgcgtacc gcctgggtta
      781 atgcagttga cgcgcaggcg ctgctgatat tcatcggcca gtacctgcat catcccttcg
      841 gtggcaaatt tcgacgctgc atatgcgccc cagttggctc gtccctgacg tccaacgctt
      901 gatgaagtaa agaccagtga accggcgtcc gatttgagta ataaaggaag cagtgcctgg
      961 gtgagcataa aggtggcatt aacgttgacc tgcatgacgt cctgccagac ctgcggattt
     1021 tgttcgctca tagggcaaac atcgccgagc aatccggcat tatgcaaaac accatccaga
     1081 cgcggataat taacggcaat gcgctgtgcc agttgttggc aatcttcgga cgtgcaggtc
     1141 agcaaatcgg gaataaccac tgtggctgac gtccagtttc ttcgtttatg tggctggcta
     1201 cctgacgtaa tttttcttca ttacggccca aaagaatcac tgtcgcacca tagcgtgcat
     1261 acgtcatcgc tgcttcacga ccaattccat cgctggctcc cgtcaccagg ataatgcgat
     1321 cattgagtaa atcttgtttt ggctggtaat gcatggctac tcctcaacga cgttgtctgt
     1381 cgcgctgcca gtgtacttta tgactttggg gctttatgcc tgaaacagaa gggcatttca
     1441 atcagcctgg ggcgataaca ccgcaaaatg gatactttgt catactttcg ctgcaataac
     1501 atctctgcga gacggctaaa catgcctgtt gtaaactgtg agccaaagcg ttgtttaacc
     1561 aaggtgggga ctcgtggaat tgttgtctga atatggtttg tttttggcga aaatcgttac
     1621 cgttgtgcta gcgattgcgg cgattgccgc cattattgtc aatgttgctc aacgtaataa
     1681 acgccagcgt ggcgagttac gagtcaacaa tctcagcgaa cagtataagg agatgaaaga
     1741 agaactggcc gcggcgctga tggacactca tcagcaaaaa cagtggcaca aagcgcagaa
     1801 gaaaaagcac aagcaagaag cgaaagccgc aaaagcgaaa gctaaactgg gcgaggtggc
     1861 aactgacagt aaacctcgcg tctgggtgct ggattttaaa ggcagcatgg acgcccatga
     1921 agtgaactcg ctacgtgaag agataacggc ggtactcgca gcattcaaac cgcaggatca
     1981 ggttgtgctc cgtctggaaa gcccaggtgg catggtgcat ggttacgggt tggcggcttc
     2041 gcagttgcag cgtctgcgtg ataaaaacat tcctttaact gttacggtag acaaagtcgc
     2101 tgccagcggc ggttacatga tggcctgtgt ggcggacaaa attgtttccg caccgtttgc
     2161 tattgtgggt tccattgggg tggtggcgca aatgcccaac tttaaccgct tcctgaaaag
     2221 caaagatatt gatatcgaac tgcacaccgc cgggcagtat aagcgcacgc tgacgttgct
     2281 gggtgaaaat accgaagaag ggcgggagaa attccgcgaa gagttgaacg aaacgcatca
     2341 gttatttaaa gattttgtga agcgtatgcg tccgtctctg gatattgaac aggtggcaac
     2401 gggtgaacac tggtacggac aacaggcagt agagaaaggc ctggttgatg aaatcaacac
     2461 cagtgatgaa gttattctta gcctgatgga aggccgggaa gtggtcaatg tacgctatat
     2521 gcagcgtaaa cgactcattg accgattcac cggcagcgcg gcagagagcg ccgatcgatt
     2581 gttgttacgc tggtggcagc gtgggcaaaa gccgttgatg taaaagacaa acgcgaggat
     2641 aagacctcgc gttttgnnnn nnnnnncagg tgtttgaaca tattaaacac cgcggtgctt
     2701 ttggcagttg gtaatccttg ttcatctaaa aagtagtcgc cggtaaatac cagcacgcca
     2761 ttacgctgcg tgacgccggt ggcttcaatc cctgcgagcg tatcctcatg ctcacgaatg
     2821 attttgttgg cctctttcaa cagcgtttcg cggtcgatgg gttgagtctc tttgttcatt
     2881 atttactcct taaacaagga cattagtcta cgccaggcat ggcttgcaga caaatatacc
     2941 acgctggtgg caagagcgcc ttactggcaa ctttggattt tgcatgctaa taaagttgcg
     3001 tatcggattt tatcaggtac agtgtgacgc tttcgtcaat ctggcaatag atttgcttga
     3061 cattcgacca aaattccgtc gtgctatagc gcctgtaggc caagacctgt taactcagtc
     3121 acctgaattt tcgtgagcag agtcacgaca aggggttgat atccgcagag agcgagtcca
     3181 tatcggtaac tcgttgccag tggaaggttt atcaacgtgc gacgcattcc tggaagaatc
     3241 aaattaggta aggtgaatat gggtaaagct cttgtcatcg ttgagtcccc ggcaaaagcc
     3301 aaaacgatca acaagtatct gggtagtgac tacgtggtga aatccagcgt cggtcacatc
     3361 cgcgatttgc cgaccagtgg ctcaactgcc aaaaagagtg ccgactctac ctccaccaag
     3421 acggctaaaa agcctaaaaa ggatgaacgt ggcgctctcg tcaaccgtat gggggttgac
     3481 ccgtggcaca attgggaggc gcactatgaa gtgttgcctg gtaaagagaa ggtcgtatct
     3541 gaactgaaac aactggctga aaaagccgac cacatctatc tcgcaaccga ccttgaccgc
     3601 gaaggggaag ccattgcatg gcacctgcgg gaagtgattg gtggtgatga tgcgcgctat
     3661 agccgagtgg tgtttaacga aattactaaa aacgcgatcc gccaggcatt taacaaaccg
     3721 ggtgagctga atattgatcg tgttaatgcc cagcaggcgc gtcgctttat ggaccgcgtg
     3781 gtggggtata tggtttcgcc gcttctatgg aaaaagatcg ctcgtggtct gtctgccggt
     3841 cgtgtgcagt cggtggcggt ccgcctggtg gtcgagcgtg agcgtgaaat taaagcgttc
     3901 gtaccggaag agttctggga agtcgatgcc agcacgacca cgccatctgg tgaagcgttg
     3961 gcgttgcagg tgactcatca gaacgacaaa ccgttccgtc cggtcaacaa agaacaaact
     4021 caggctgcgg taagtctgct ggaaaaagcg cgctacagcg tgctggaacg tgaagacaaa
     4081 ccgacaacca gtaaacctgg cgctcctttt attacctcta cgctgcaaca agctgccagc
     4141 acccgtcttg gttttggcgt gaaaaaaacc atgatgatgg cgcagcgttt gtatgaagca
     4201 ggctatatca cttacatgcg taccgactct accaacctgg gtcaggacgc ggtaaatatg
     4261 gttcgcggtt atatcagcga taattttggt aagaaatatc tgccggaaag tccgaatcag
     4321 tacgccagca aagaaaactc acaggaagcg cacgaagcga ttcgtccttc tgacgtcaat
     4381 gtgatggcgg aatcgttgaa ggatatggaa gcagannnnn nnnnnatggc tggacgaaag
     4441 tgaagcctgc acagcgtaaa ggcgatgaag atcgtatctt accggcagtc gataaaggcg
     4501 atgctctgac gctcgttgaa ctgacaccag cccagcactt taccaagcct ccagcccgtt
     4561 tcagtgaagc atcgctggtc aaagagctgg aaaaacgcgg tatcggtcgt ccttctacct
     4621 atgcgtcgat catttcgacc attcaggatc gnnnnnnnnn nacgacttca ccgcgcagat
     4681 ggaaaacagc ctcgaccagg tggcaaatca cgaagcagag tggaaagctg tactggatca
     4741 cttcttctcg gatttcaccc agcagttaga taaagctgaa aaagatccgg aagagggtgg
     4801 tatgcgcccg aatcagatgg ttctgaccag cattgactgc ccgacttgtg gtcgcaaaat
     4861 ggggattcgc acagcgagca ccggggtatt ccttggctgt tctggctatg cgctgccgcc
     4921 gaaagagcgt tgcaaaacca ccattaacct ggtgccggaa aacgaagtgc
//
LOCUS       DAWDRC010000280         4960 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7410_length_4960_cov_2.152294, whole genome shotgun sequence.
ACCESSION   DAWDRC010000280 DAWDRC010000000
VERSION     DAWDRC010000280.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7410_length_4960_cov_2.152294"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1032..1041
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1648..1657
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2607..2616
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1123 a         1233 c         1319 g         1255 t
ORIGIN      
        1 ccaataatcc gatttttcac caccagtggc agaacaattt cagaattact cgtcgcatca
       61 caggcaatat gcccgtcaaa cgcctgaaca tcctcgatac gctgcacttg attgcgggca
      121 accgcagtgc cgcatacgcc gcgcccaacg ggtatccgga cacaggcaat tttgccctga
      181 aatggtccga gcaccagtgt atcgtcctca agcaaataaa aacctgccca gtttacgtca
      241 gtgagacgct catataacaa cgcactggtg ttcgcaagcg ttgccagaaa actggtttct
      301 cccaccatca gcgcgttaaa gtcgcgattt aaatccgcgt aaaattctgc tttgttcatt
      361 atataatcac ttggttgtct tacctggaac tgccagccta ttaaaataag cattaaatgc
      421 gttaatgctc aagatcattc ccatcatggg ttaagattaa tgttaattct tattacattt
      481 ggcacgtcat ggctcttaac acaccacaaa ttacgccgac aaagaagata acagtgaggg
      541 ctatcggcga ggaactgccg cgtggtgatt accaacgttg cccgcaatgt gacatgctgt
      601 ttagcctgcc cgagataaat tctcatcaaa gtgcctattg tccgcgctgt caggcaaaaa
      661 ttcgtgacgg gcgcgactgg tcgctaacgc gcctggcggc aatggccttc accatgctgt
      721 tgcttatgcc gtttgcctgg ggcgaaccgc tgttgcatat ctggctgtta ggcattcgta
      781 ttgacgccaa cgttatgcaa ggcatctggc aaatgaccaa acagggcgat gcgataacgg
      841 gggcgatggt ctttttctgc gttatcggtg cccccctcat tctggtgacc tccatagctt
      901 atttatggtt tggtaaccga ttgggaatga atttacgccc ggtactgctg atgcttgagc
      961 ggctgaaaga gtgggtgatg ctggatatct acctggtcgg cattggcgtt gcttctataa
     1021 aggtacagga tnnnnnnnnn ntatgcccat atccaggcgg gtgtcggctt gttctctttt
     1081 gtggcgttgg tgattttaac gacggtgacg ttgtcacatc ttaatgttga agaactgtgg
     1141 gagcgatttt atccacagcg ccccgctacc cgtagggacg agaaactccg tgtctgtctt
     1201 gggtgccatt ttaccggcta tcctgatcag cgtggtcgct gcccgcgttg ccatatcccg
     1261 ctacgcctgc gtcgccgtca tagtctgcaa aaatgctggg cggcgctgtt agcgtcaatc
     1321 gttttgttgt tacctgccaa cctgttgcct atctctatca tttatctgaa tggtggtcgg
     1381 caggaagata cgattctttc cggaattatg tcgctggcaa gtagcaacat tgcagttgcg
     1441 ggaatcgtgt ttatcgccag tattctggta ccgtttacta aagtgatcgt catgttcact
     1501 ttactgttga gtattcattt taaatgccag caaggtttac gcacacgcat tctgttactg
     1561 cggatggtga cctggattgg tcgctggtcg atgctcgacc tgtttgtcat atctttaacc
     1621 atgtcgctga ttaatcgcga tcaaatcnnn nnnnnnngat caaatcctcg cttttactat
     1681 ggggccggct gcgttttatt tcggcgcagc ggtaattttg actattcttg ctgtggaatg
     1741 gctggacagc cgcttacttt gggatgcaca tgagtcagga aacgcccgct tcgacgactg
     1801 aagcgcagat taaaaataaa cgccgtatct cacctttctg gctgctgccg ttcatcgcgc
     1861 taatgattgc tggttggctg atttgggaca gttatcagga ccggggcaat actgtcacca
     1921 tcgactttat gtcggcggat ggtattgttc caggccgtac gcctgttcgt tatcagggcg
     1981 ttgaagttgg aacagtgcag gatatcagcc tcagcgacga tcttcgtaag attgaagtca
     2041 aggtcagcat caagtccgat atgaaagatg cgctgcgcga agagacacag ttctggttgg
     2101 tgacgccaaa ggcctcgttg gcaggtgtct ccgggctgga cgccctcgtc ggtggtaatt
     2161 atatcggcat gatgccgggt aaaggtaaag agcaggatca ctttgtcgca ctcgacaccc
     2221 aaccgaaata tcggctggac aatggcgatc tgatgatcca cctgcaagcc cccgatctcg
     2281 gttcactgag cagtggttca ttggtctatt tccgcaagat cccggtgggt aaagtctacg
     2341 actatgccat caatcccaac aagcaaggcg tggtgattga tgtcctgatc gagcggcgtt
     2401 ttaccgatct ggtgaaaaaa ggtagccgtt tctggaacgt ttccggcgtt gatgccaacg
     2461 tcagtatcag tggcgcgaag gtgaaactgg aaagtctggc ggcactggtt aacggtgcga
     2521 ttgccttcga ttcacctgaa gagtcgaaac ctgccgaagc ggaagatacc tttggtctgt
     2581 atgaagacct ggcccatagc caacgtnnnn nnnnnngtgc cggattaacc gccgactcga
     2641 cgccgttaat gtatcagggg ctggaagtcg gacagctgac taaactggat ttaaatcctg
     2701 gtggtaaagt cactggggaa atgaccgttg atcccagcgt cgtcaccctg cttcgtgaaa
     2761 atacccgcat cgaattacgt aatccgaaat tatcccttag cgatgctaat ctcagtgccc
     2821 tgctgaccgg caaaaccttc gaactggtgc ccggcgatgg cgagccacgt aaagagttcg
     2881 tggttgtgcc aggcgaaaaa gccctgctgc aagagccgga tgttctgaca ctgaccctga
     2941 cagcaccaga aagttacggt attgatgctg gtcagccgct cattcttcac ggcgtgcagg
     3001 taggccaggt tatcgatcgt aaactcacca gcaaaggcgt cacctttacc gtcgccatcg
     3061 agcctcagca tcgcgaactg gtaaaaggcg atagcaaatt tgtcgtcaac agccgcgtcg
     3121 acgtgaaggt agggctggat ggcgttgagt ttctcggtgc cagcgcctca gaatggatca
     3181 atggcggaat acgtattctg ccgggcgata aaggcgagat gaaagccagc tatccactgt
     3241 atgccaatct ggaaaaagcg ctggagaaca gccttagcga tttacccacc acaaccgtga
     3301 gtttgagtgc agagacgctg ccggatgtgc aggcaggatc ggtagtgctg taccgtaaat
     3361 ttgaagttgg agaagttatt actgtgcgtc cgcgagctaa cgcttttgat ctcgatctgc
     3421 atatcaagcc ggagtatcgc aaccttctga ccagcaacag cgtgttctgg gcagaaggtg
     3481 gggcgaaagt tcagctgaat ggtagtggtc tgactgtaca ggcatccccg ctctccagag
     3541 cattaaaggg ggctattagc ttcgataacc tcagcggtgc cagcgccagt cagcgtaaag
     3601 gcgacaagcg tattctgtat gcttccgaaa cagcggcccg tgcggttggc gggcaaatta
     3661 cgcttcacgc tttcgatgcc ggaaaactgg cggtcgggat gccaattcgc tatctcggta
     3721 ttgatatcgg gcaaatccag acgctggatc tgattaccgc gcgcaatgaa gtacaggcaa
     3781 aggcggtgct ctatccggaa tatgtccaga cctttgctcg cggtggtacg cgcttctcag
     3841 tggtcacacc gcaaatttcg gcagctggcg ttgagcatct tgatactatc ctccagccgt
     3901 atatcaacgt cgaaccaggc cggggcaatc ctcgccgcga ctttgaatta caagaggcca
     3961 ccattactga ttcgcgttac ctggatggct taagcattat tgttgaagcg ccggaagccg
     4021 gttcgttagg tatcggtaca cctgtgctgt tccgtggtct ggaagtaggt acggttaccg
     4081 gaatgacgct ggggacattg tcagatcgcg tgatgattgc gatgcgcatc agtaaacgct
     4141 atcaacacct ggtgcgtaac aattccgtct tctggttggc atcgggttac agtctggact
     4201 ttggtctgac gggcggcgta gtgaaaaccg gcacctttaa ccagtttatc cgtggcggca
     4261 tcgccttcgc cacgcctccg ggtacgccac tggcaccgaa agcccaggaa ggcaagcact
     4321 tcctgttgca ggaaagtgaa ccgaaagagt ggcgtgaatg gggaactgcg cttcccaaat
     4381 aatgcccact gctccggcgt gcctgcgccg gagcgtttat gctaaactgc gcgcctgttt
     4441 ttttgccagt ggtatatgct cgtggcccaa cacaccgttt atttcccgga cgcctttctg
     4501 acacaaatgc gcgaagcgat gccttcgacg ctctcttttg atgattttct tgccgcctgt
     4561 cagcgcccgt tgcgccgcag cattcgcgtt aatacgctga aaacctccgt tgctgatttc
     4621 ctgcaattaa ctgctcctta tggctggacg cttacgccaa ttccgtggtg tgaagaaggt
     4681 ttctggattg aacgcgacag tgaagatgca ttgccgttgg gtagtaccgc cgagcattta
     4741 agcggcctgt tttatattca ggaagccagt tcaatgttgc ctgttgccgc cttgtttgct
     4801 gacggtaatg caccacagcg ggtgatggat gtcgctgctg caccaggttc caaaacgacg
     4861 caaattgctg cgcggatgaa taacgaaggg gcaatccttg ccaatgagtt ttccgccagt
     4921 cgggtaaaag tgttacatgc caatatcagc cgctgtggca
//
LOCUS       DAWDRC010000281         4941 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7437_length_4941_cov_2.439828, whole genome shotgun sequence.
ACCESSION   DAWDRC010000281 DAWDRC010000000
VERSION     DAWDRC010000281.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7437_length_4941_cov_2.439828"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3683..3692
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1207 a         1204 c         1277 g         1243 t
ORIGIN      
        1 gcctgttgcg ctacgtatca gggctacatc attttttaat tcattgaatt taattggttt
       61 tgcaggccgg ataaggcgtt aacgccgcat ccggcataaa cacaggcact tgtcaccagt
      121 ctgaaaccag cgtcataaaa atccggttgc tactcgacaa gacgtccatt acccgagaat
      181 tttgctggct tcgcgcgcta cattatccat ctcgtccaac agttccagca gctccggctc
      241 taaatcttct tctttagtac cgctacgtag ctgttgttcg atcagttggc agagattctt
      301 catacgcggt acaccgctat agccgcaact gccatgcagt ttatgaatca aatcaaccag
      361 gccttccggg ttttctccaa ccagctgttc ctcaactttg ttgcgaactt caggcaggaa
      421 atcgagtaac atttgcagca tatcgcgcgc taaatcggtt tttcctgctg cctggcgtag
      481 tgccagttgc cagtcgaggg tcgcattcgg gttgaccaca atttcgttga cttcgggcgt
      541 cacgacgcga gaggaaatac cgctgccagg cttgtagcgc aacagcaaat tatgcaatcg
      601 ctcttcttcg atcggtttgg ccagataatc actcatcccc gcgccaagca gtttctcttt
      661 ttgcccggcc atcgcatgcg ccgttaccgc gataaccggc gtttgctgct gatgcgggag
      721 ctggtggatg agctcacatg cccgaatgcc gtccatgtcc ggcatttgaa tatccattaa
      781 gattaaatcg aacggcatct gtttcgcccg ttcaaccgcc tgatgcccgc tatcgcaaag
      841 ttccacatgt tgcaccatat cttccagcaa tgcgccgata agtttcaggt tagcggggtt
      901 gtcatcaacc gccatgactg tcattgccag cttactttca tcggttacag gcaaaagcgt
      961 gttttgtttg tgatgacaaa attctgtcag ggcaggcaac aggcgcgtag gtgttaatgg
     1021 tttcagcaga cacgcgccga taccatcttg cttaagtttt tcagcattga cttgtgcatg
     1081 gcaaggaagt gccagcatca ggaaatcggt catcgatact gctttcgcta atcgctcatg
     1141 ttgcattgtt agcggctcgc ggaaggtcac cgctatgcct aataacatca tgtcgtaatg
     1201 cgcgggaggc agcgcggaga acgttgggct ataaaccact tccagcggcg tttcacttaa
     1261 aatatccagc gtgcattgtg ctgctgcgga gtttggttcg acataggcca ggcgcttacc
     1321 agcgaggcac tgggtggatg gcccttcgat aataatgttc gggttcagat cgagattaat
     1381 gtggaaccag aaagttgaac cacgattcgg caggctatgg aacgaaatat caccgcccat
     1441 ttcattaacc agtttttgtg taatcaccag ccccagcccg gtgccaccat gacggcggga
     1501 aatactggca tcagcctgtc tgaaggcctg gaataagcgc gattgatcgc gttcaggaat
     1561 accaatgccg gtatcccgaa tctgcacttc aatttgcact ttggtattac tcagcgcacg
     1621 tttttctacc agaatatcaa tgttgccatt ctcggtgaat ttaattgcat tccctaccag
     1681 gttagtgatg atttgctgta atcgtagtgg gtcgccgatc acgttatcag gcacgtcgct
     1741 tttaatattg agcgtcagtt ccagcccttt atcgtgagaa gaatgtgcca gcagggtaac
     1801 gacttcatcc agcgtgctgc gtagtgggaa tggaatactt tccagaatca gcttaccggc
     1861 ttccagtttc gagaagtcga gaacatcatt aataattgcc agcaaattat ttgccgaacg
     1921 ttcaatcgta ttcaggtgat cgcgctgcgt tggtgttaat tctgttttca gcgtcaggcg
     1981 ggtaaagcca ataacaccat tcagtggtgt acgcagctcg tgtgacatat ttgccagaaa
     2041 ctcggattta atacgcgccg cttcctgggc gcgctttttc gccagatcca actcaacgtt
     2101 ctgaatctcc atttgctcca gcgtctcacg cagatcggac gtcgcctggt cgatattgtg
     2161 ttgcatctct tcgtgataag ccgccagcga cattgccatc gagttgatac cgtttttcag
     2221 catatccagc tcgccgagca taaatccttc cacccggctg tcgagttgcc cgcgacggat
     2281 gcggtcgacg gtattcacca tgttgcgaat cggaccggtt acatcgcgca ttaagcgcca
     2341 gccaaaaata agcgcaatac cgatacaaaa cagcatcatc acgctggaaa taaagatctc
     2401 tttatattgc tgcaagcgaa ccgatttaag atccagctcc agcgcaatat atcccagcat
     2461 attttgacta tttttggcat cgctactggg cgattcgtcg ggggagtaac tttcagaaat
     2521 aatcggcgtg cggaggatca taatatcgcc gtcacgagtg acagtgagct ggcgaggaaa
     2581 cggcacgttg ctgccgagct gcattgatga gggatcaaga tgaaaattgg aggtgacaaa
     2641 gagtcggtta ttttcatcat aaaccgaaat cgcgcgaaca atatcagaat ggcgacgatg
     2701 cagtacgctt attaactgac cgatagattc gcgattttgc agactcatgc catattcagt
     2761 agaaactgca agcggctcaa taatgctggc accggcatct tccagttgac gctgcaagtc
     2821 gttatagcga tgcacgacga aaaagatact cagcaataaa ccaataagga cggtcggtgc
     2881 caggatcaga atcatcatgc gtgcgcgcag gctgtagttg gtcatggagt tccgttatgg
     2941 gacaattaag gtcacactgt taaattgaga aagtctcgcc aatggcgcaa ttctactctg
     3001 caaaacgacg cacgacgacg cgtcagatca taaccgtttc agtcaacgac ctcgactctt
     3061 ttggtcaggg cgtggcgcga cataacggca aaacgttgtt tatccccgga ttattgccgc
     3121 aggaaaacgc ggaagttact gttactgaag ataaaaaaca gtatgcccgc gctaaagtcg
     3181 tacgtcggtt aagcgatagc ccggaacgcg aaacgccacg ctgtcctcat tttggcgtat
     3241 gcggcggctg tcagcaacaa cacgccagtg tggatttaca gcagcgaagc aaaagtgcgg
     3301 cactcgcccg attaatgaaa cacgaagtct ctgaagtgat cgccgatgtt ccctggggtt
     3361 atcgccgtcg cgcgcgttta agtctgaact acttaccgaa aacacagcaa cttcagatgg
     3421 ggtttcgcaa agcgggctcc agtgacattg tcgacgtgaa acaatgcccc attttagtgc
     3481 cccaacttga agcattgctg cccaaagtca gggcatgcct gggcagctta caagctatgc
     3541 gccatcttgg tcatgttgaa ctggtacagg caaccagcgg cacgctaatg attttgcgcc
     3601 ataccgcacc gctaagttcg gcagatcgcg aaaaactgga atgcttttcg cattctgaag
     3661 gcctggatct gtatctcgcc ccnnnnnnnn nnctcgcccc cgatagtgag atactcgaaa
     3721 ccgtctctgg tgagatgccc tggtatgact caaatgggtt gcgcttaact tttagcccgc
     3781 gcgattttat tcaggtcaat gcgggtgtga accagaaaat ggtagcgcgt gcgttggaat
     3841 ggctggatgt ggaacctgaa gattgcgtac tggatctgtt ctgcggtatg ggcaacttta
     3901 cactgccatt ggcgacacaa gctgccagtg tggtgggtgt agaaggtgtt ccggcgctgg
     3961 tggaaaaagg ccagcagaat gcgcgtctta atggcttaca gaatgtgacg ttttatcacg
     4021 aaaatcttga agaagatgtc acaaagcagc cgtgggcgaa aaacggcttc gataaagtgt
     4081 tgttggaccc ggcgcgagca ggtgccgcag gtgttatgca gcaaattata aaactggaac
     4141 ctattcgtat agtttatgta tcctgtaacc ctgcaacgct ggctcgggat agcgaagcgt
     4201 tattaaaagc aggatatacc attgcgcgac tggcgatgct ggatatgttc ccacacacgg
     4261 gacatctgga atcgatggta cttttctcgc gcgttaaata gttacgattt gccgatttcg
     4321 gcaggtctgg tccctaaagg agaggacgat ggttgcggta agaagtgcac atatcaataa
     4381 ggctggtgaa tttgatccgg aaaaatggat cgcaagtctg ggtattacca gccagaagtc
     4441 gtgtgagtgc ttagccgaaa cctgggcgta ttgtctgcaa cagacgcagg ggcatccgga
     4501 tgccagtctg ttattgtggc gtggtgttga gatggtggag atcctctcga cattaagtat
     4561 ggacattgac acgctgcggg cggcgctgct gttccctctg gccgatgcca acgtagtcag
     4621 cgaagatgtg ctgcgtgata gcgtcggtaa gtcggtcgtt aaccttattc acggcgtgcg
     4681 tgatatggcg gcgatccgcc agctgaaagc gacgcacact gattctgttt cctccgaaca
     4741 ggtcgataac gttcgccgga tgttattggc gatggtcgat gattttcgct gcgtagtcat
     4801 caaactggcg gagcgtattg ctcatctgcg cgaagtaaaa gatgcgccgg aagatgaacg
     4861 tgtactggcg gcaaaagagt gcaccaatat ctacgcgccg ttggcgaacc gtcttgagat
     4921 tgggcaactg aaacgggagc t
//
LOCUS       DAWDRC010000282         4929 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7457_length_4929_cov_2.054780, whole genome shotgun sequence.
ACCESSION   DAWDRC010000282 DAWDRC010000000
VERSION     DAWDRC010000282.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7457_length_4929_cov_2.054780"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1285 a         1321 c         1279 g         1044 t
ORIGIN      
        1 tctaatcctc tggggtatca ctaccgtaat ttgaaccggc aagataatgc cgaagttctg
       61 taaataagta aagatttgcg cgctaaatcg caacaaacag gttcggcaca ttactccgaa
      121 aacacacggc taagccgcac caaaagcgca acgtataagg gagcggtgag ataaacgatg
      181 ggcgttacct gacgcgaaaa attccttatc ggcagcgggg taatgagcgt aaccaactct
      241 gcgaccgcaa ttataacact ctggggagaa atgtgccgaa aacattcatt cttgtggtga
      301 aaacaagcat cgaggtaccc agaaattatt cggcaatcgt ccgaggcgca tttgattgag
      361 ataattaagg taatcccggc ggggaatttc gcaggcacca agcgatgctg tgtgatcgtt
      421 aaggacctgg cagtcgataa gtttaccgcc atgacggata aattcatcac agaagaccag
      481 aagcgccgtt ttggacgcat tttccatccg gctgaacatg gactcgccac aaaatagtgt
      541 tccctgggcc acgccgtaca taccgccgac aagcccatct tcacgccaga cttcaatgga
      601 gtgggcatga ccgagttcgt gaaggcgatg gtaggcttcg accacgccac gcgtgatcca
      661 ggttccttct tcgcgatcgc tggcacagcc ttcgatgacc tgaccaaaag cgtaattcat
      721 cgtgacacga tagggtgagc gtttatgaaa tcgcttcata ctacggctga tatgcagtga
      781 ttctggccat aacaccgcgc ggggatcggg cgaccaccag aggatggggt cgccaggaga
      841 aaaccacggg aaaataccac gctgataagc cattaatagg cgcgcagggc taagatcgcc
      901 ccccagtgcc agcaggccgt taggctcacg taatgcgcct tccggggaag ggaaggctat
      961 tgaatggcga gaaagctgaa ccaggcgcat gaccgcaaaa ctccacgcaa gtcggatcgt
     1021 tcaataatag cttacaaacc ctgcttaaac tggtaataac gcccctgtct ggcaagcagt
     1081 tctgcgtgag taccttgctc aataatttgc ccgttgtcca tcactattat ttgttggaaa
     1141 cgagagagtc cgcgaagtcg atgggtgacc attaacaccg ttttctcgca catcatttct
     1201 gcaagcaatt caaggatctg actttcggtt gtggcatcta agccttctgt aggttcatcc
     1261 agcaatacca gcggcgcatc atgtaacagc gcacgggcga tagccagacg gcgcagttca
     1321 ccaccggaga gctggcgtcc gccttcacct aaccaactgt tgagacctgc atcctcgagc
     1381 agcttctcca gaccaacgcg acgcaagatc tcctccagag cctcatcact actaccaggc
     1441 gaggcgagta acagattatc acgcagcgtg gcgctaaaca gatgcactcg ctggggaaca
     1501 acgctgatgg tctgtcgtag agccgcttca ttcaggctgg ctatgggact atcgttaagc
     1561 aaaatctcgc cctgttgcgg gtcccatgcg cgggtcagca gttgtaacag tgttgatttg
     1621 ccgcatccgg ttcgcccgag aatcgcaata tgttccccgg cgtttacctg aagataaatc
     1681 cctttaagtg cctgttggga ttgctccgga taagtgaact gaacatcccg taatgtcagc
     1741 gaaacgcgat cggcaacacg agtttgggta tcagggaagg tgacttccgg tttttgatcc
     1801 gttaaatcag tgatccgtac ggcagaggca atgacttgcc ccagatgctg aaatgcaccc
     1861 gttactggtg ccagcgcttc aaacgcggct aacgcgcaga agacaaacag ggcaattaac
     1921 gcgccgggtt gagcattgcc gccaacgccg ccagacgcca tccacagcat caggatcacc
     1981 gctaacgcgc caatgagcag cattatcgct tgcgacaatg cggtcagttc agattgacgg
     2041 cgttgcgctt ccagccactg aatttctgta ttctctaatt gcgtgcgata acgatcgctg
     2101 gcaccaaaaa tggtcagctc agcttgccct tgcagccagg ccgtcagttg ttggcgatac
     2161 tgcccgcgaa gatgagtcag attttgcccg gtgctttttc ccgcacgata aaacagcggt
     2221 ggcatcagga aaagcgtcag taacataatg ccgcccagcg taaaggcgag ggtgaaatca
     2281 aggaaactta acccgattgt caccaccata atcaccacaa aagcgcccac cagcggcgag
     2341 ataacgcgca ggtaaagatg atcgagcgta tcaacatccg ccaccacgcg gttgagtaat
     2401 tcgccctgac gatagcgcgc cagtccggca ggggagaggg gcagcaattt gctgaaggtg
     2461 tatatgcgca gatgctgcaa cacgcggaaa gtcgcgtcgt gacttaccag acgttcaaaa
     2521 taacgcccgg cagtacgggt gattgctgcg ccacgcacgc ccgcagcggg tagcatatag
     2581 ttgaagctgt acagtccggc aacccccgca accgctgagg cggagaggaa ccagcccgaa
     2641 agtgtcaaca gaccgatact ggcgagcagc gtgacaattg ccagcacgat accaagactt
     2701 aacatccatt tatgacgttt atacagtgcc agatagggta gcaaagcgcg catttaaatc
     2761 tcctcctgac gatgggccag taatgtggcg aatgggccgc cagccacact taattccgcg
     2821 taacgtcctt gctcaataat ctgaccatcc tgcattaccc aaatgacatc ccagtcagca
     2881 agatcttcta actggtgggt gaccattaac gttgtctggc gcagagaggc ggcattcagc
     2941 gcctccatta cgcgctgttc actgtgagca tcaaggctgg cagcgggttc atccaacagt
     3001 aatagcgaac agggatttag taacgcacgg gccaccgcca cgcgctgcgc ctgccccacg
     3061 gaaaggcggg cagcctggtc gccaacaggc gtatcaacgc cttgtggcag gagcggtaga
     3121 aactcactga cccaggcgtt atccagcgct gtttgtaact cttgttcgct ggcatcaggt
     3181 cgcgccagta gtacgttatc ccgtaatgtt gctgccggta attgtgggtt ttgcccaacc
     3241 caggagagat gtttacgcca tgattctggt gataaatcgc gtaattctat cccgttgatt
     3301 cgaccaacca acactgctcg ttggcctgct ggcaaagtaa aattcagcgg tccggccagc
     3361 gttttacctt ccggcgacgt gataaacaga tcctcggctt caatggtcac cggatcggtc
     3421 gatgctaatt ctgcttcacc gcgctgcgga tgggctagcg gggtttccat aaacgttttc
     3481 aaactgtcag ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgt
     3541 aatggctgga aaaactctgg cgcaaggatc agggccagaa aacccgcagc cagcgtcacg
     3601 ccagtatcgt agtgaccaaa atccagctcg ccgaggtagg aaaaaccaaa gtagaccgcc
     3661 accagagcaa tcgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagc
     3721 cgtagcactt ccattgtccg ttgacggaaa tcttccgaag cagaacgaat actttcaatt
     3781 tcagcttcac cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa
     3841 tgcccactta agcgagcaag agcgagaaag ttacgtcggt tagcatcggc agcccccatt
     3901 ccaaccagcg ccataaataa cggaattagc ggtgcagtgc ccagcagaat gagcgccgca
     3961 gcccagttag aggggaagat agccaccaca atcagcagcg gcaccgacac tgccagcgcc
     4021 atttgcggca ggtagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccagc
     4081 gtcgcccagc tccccgctgg tttaccctga atccacgctg gccctgcttg ttgcagacgg
     4141 tcgagaacct gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt
     4201 tcgcgtaacc agaccaccca tgcgcgcagt acaaaggtca gaaacagtaa cgtaaaggga
     4261 agcagcaggg cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg
     4321 aaccaggcct gggaaatgat caatatgccg ctcacaaagc ccagcagacg agaaatattc
     4381 agccaacgtt gggagatgac gctttgctgt tttaaccagc gggttaattc tttttgacgg
     4441 gatttattca ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac
     4501 aacggggaaa aaataaaggc gacccatagt cgcatggtgt cgccttcttt acttttgtta
     4561 ctgatttgta aaattatttt gcgtcagcta aaccgtcaag gtagcgttcc gcatcaagag
     4621 ctgccatgca gcctgtacca gcagatgtaa tggcctggcg ataaatgtga tccatcacgt
     4681 cgcctgcggc aaagacgcca gggatgctgg tctgggtggc attaccatga atacccgact
     4741 gtactttgat gtagccgttt tccagttcca gctgcccttc gaaaatggca gtattcgggc
     4801 tgtgaccgat agcaacaaac agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt
     4861 gcgtatcgcg cagacgaacg ccagtgacgc ccatctgatc gccggtcact tcttccagcg
     4921 tacggttga
//
LOCUS       DAWDRC010000283         4906 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7488_length_4906_cov_2.447124, whole genome shotgun sequence.
ACCESSION   DAWDRC010000283 DAWDRC010000000
VERSION     DAWDRC010000283.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7488_length_4906_cov_2.447124"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1311 a         1130 c         1160 g         1305 t
ORIGIN      
        1 cataacgttg aagacgaagc tgccgaccag cgttttatgt cagtgcggac atcagcactg
       61 cgtgaacaat ttgcctggct gcgcgagaac ggttatcaac cggtcagtat tgctcaaatt
      121 cgtgaagcac atcgaggagg aaaaccgcta ccggaaaaag cagtagtgct gacttttgat
      181 gacggctacc agagttttta tacccgcgtc ttcccaattc ttcaggcctt ccagtggcct
      241 gctgtatggg cccccgtcgg cagttgggtc gatacgccag cggatgaaca agtaaaattt
      301 ggcgatgaga tggtcgatcg agaatatttt gccacgtggc aacaagtgcg agaagttgcg
      361 cgttcccggc tcgttgaggt cgcttctcat acatggaatt ctcactacgg tattcaggct
      421 aatgccaccg gcagcttatt gcctgtatat gtaaatcgtg catattttac tgaccacgca
      481 cggtatgaaa ccgcagcaga ataccgggaa agaattcgtc tggatgctgt aaaaatgacg
      541 gaatacctgc gtacaaaggc taaggtaaat ccacacgttt ttgtttggcc ttatggcgaa
      601 gcgaatggca tagcgataga ggaattaaaa aaactcggtt atgacatgtt cttcaccctt
      661 gaatcaggtt tggcaaatgc gtcacaattg gattccattc cgcgagtatt aatcgccaat
      721 aatccctcat taaaagagtt tgcccagcaa attattaccg tacaggaaaa atcaccacaa
      781 cggataatgc atatcgatct tgattacgtt tatgacgaaa accaccagca aatggatcgc
      841 aatattgatg tgctaattca gcgggtgaaa gatatgcaaa tatcaaccgt gtatttgcag
      901 gcatttgctg atcccgatgg tgatgggctg gtcaaagagg tctggtttcc aaatcgtttg
      961 ctaccaatga aagcagatat ttttagtcgg gttgcctggc aattacgtac ccgctcaggt
     1021 gtaaacatct atgcgtggat gccggtatta agctgggatt tagatcccac attaacgcga
     1081 gtaaaatact taccgactgg ggagaaaaaa gcacaaattc atcctgaaca atatcgccgt
     1141 ctctctcctt tcgatgacag agtcagagca caagttggca tgttatatga agatcttgcc
     1201 ggacatgctg cttttgatgg catattgttc cacgatgatg ctttgctttc agattatgaa
     1261 gatgccagtg caccggctat cacggcttat cagcaagcag gctttagcgg gagtctgagc
     1321 gaaattcgac aaaacccgga gcaatttaaa cagtggaccc gctttaaaag tcgtgcgtta
     1381 actgacttca ctttagaact tagtgcgcgc gtaaaagcca ttcgcggtcc acatgttaaa
     1441 actgcacgaa atatttttgc acttccggta atacaacctg aaagtgaagc gtggtttgca
     1501 cagaattatg ctgatttcct aaaaagctat gactggaccg ctattatggc tatgccttat
     1561 atggaaggtg tcgcagaaaa atcggctgac caatggttaa tacaattgac caatcaaatt
     1621 aaaaacatcc ctcaggctaa agacaaatct attttagaat tacaggcaca aaactggcag
     1681 aaaaatggtc agcatcaggc tatttcttcg caacaactcg ctcactggat gagcctatta
     1741 caactgaatg gagggaaaaa ctatggttat tatcccgaca attttctgca taaccaacct
     1801 gaaatagacg ttattcgtcc tgagttttca acagcctggt atccgaaaaa tgattaatcg
     1861 catcgtatcg ttttttatat tatgtctggt gttatgcatc cccctatgcg tagcgtactt
     1921 tcactctggt gaactgatga tgaggttcgt tttcttctgg ccgtttttta tgtctattat
     1981 gtggattgtt ggcggcgtct atttctgggt ctatcgtgaa cgccactggc cgtggggaga
     2041 aaacgcacca gccccccagt tgaaagataa tccgtctatc tccattatca ttccctgttt
     2101 taatgaggag aaaaacgttg aggaaaccat acacgccgct ttagcacagc gttatgagaa
     2161 cattgaagtt attgccgtaa atgacggttc aacagacaaa acccgtgcca tcctggatcg
     2221 catggctgca caaattcccc acctgcgggt cattcatctg gcgcaaaacc aggggaaagc
     2281 cattgcgctt aaaaccggag ctgccgcggc gaaaagtgaa tatctggtgt gcattgatgg
     2341 cgatgcgtta ttagaccgcg atgcggcggc atatattgtg gaaccgatgt tgtacaaccc
     2401 gcgtgtgggt gccgtaaccg gtaatcctcg tattcgaaca cgttctaccc tggtgggtaa
     2461 aattcaggtt ggcgagtatt cctcaattat tggtttgatc aagcgaaccc agcgtatcta
     2521 tggaaacgta tttaccgttt ccggtgttat tgccgcattt cgtcgcagcg ccctggcaga
     2581 agtgggttac tggagtgacg atatgatcac cgaagatatt gatattagct ggaagctgca
     2641 gttgaatcag tgggcgattt tttacgagcc acgggcgctg tgctggatat taatgcctga
     2701 aacgttaaaa gggctgtgga aacagcgcct gcgctgggct cagggcggtg cagaagtatt
     2761 cctcaaaaat atgacaaggt tgtggcgcaa agaaaacttt cgaatgtggc cgctgttttt
     2821 tgaatactgc ctgacgacaa tatgggcctt cacttgcctg gtcggtttca ttatttacgc
     2881 agtccaactt gccggtgtac cgttaaatat tgaattgaca catatcgctg cgacacatac
     2941 tgccggaata ttattgtgta cgttatgttt actgcaattt attgtcagcc tgatgatcga
     3001 gaatcgctat gagcataatc tgacttcatc gcttttctgg attatttggt tcccggttat
     3061 tttctggatg ctgagtctgg caacgacatt ggtatcattt acacgagtca tgttgatgcc
     3121 taaaaagcaa cgcgcccgtt gggtaagtcc cgatcgcggg attctgagag gttaatatga
     3181 acaatttaat tattacgacc cgacaatcgc cagtacgttt aatggttgat tatgttgcca
     3241 caaccatctt atggacatta tttgctttgt tcatattctt attcgcaatg gatctgctga
     3301 cgggttatta ctggcaaagc gaggccagaa gccgacttca gttctatttt ttgctggcag
     3361 tggcgaatgc cgtcgtgtta attgtctggg cgctgtacaa taagctacgt tttcaaaaac
     3421 agcagcatca tgcagcctac caatatacgc cgcaagaata tgctgagagc ttagcaatac
     3481 ctgatgagct ctatcagcaa ctacaaaaaa gccacaggat gagcgtacac ttcaccagcc
     3541 aggggcaaat aaaaatggtg gtttcagaaa aagcgctagt ccgggcgtaa acacccaaaa
     3601 caaagcccgg ttcgcccggg ctctgcaccg ataatacact taactgtatg catgtagcgt
     3661 acgttggcaa agtgccgaac gtacgcaatc ctctttaccg aatcggacga tcccgaccat
     3721 ttcatcttct tcgaaacgtt ccagcgcgtc acttaatccg gagcgcacgc cgcgaggcaa
     3781 atcgcattgc gtgatatcac cgttgacgat aaccgtcacg ttctccccga ggcgggttaa
     3841 aaacattttc atttgcgcgg cagtcacatt ctgcgcctcg tcaagaatga cgactgcatt
     3901 ttcaaaggta cgtccacgca tataggcgaa cggcgcaatt tccaccttcc ctatttccgg
     3961 tcgcaggcag tactgcataa aggaagcccc taagcgccgg accagcacgt cgtagaccgg
     4021 acgaaaatag ggagcaaact tttctgcgat atctccaggt aagaagccaa gatcttcatc
     4081 ggcttgcaga actggacggg tgacgataat cctgtcgaca tccttatgta tcagggcctc
     4141 tgccgctttt gctgcgctga tccaggtttt tccgcacccg gcttcgcccg tggcgaatat
     4201 cagctgctta ctctcaatag ccttcagata gtgcaattgc gcttcatttc gcgcgaggat
     4261 gggcgaggta tcgcgactgt cgcgggccat accaatggct tctacgccgc tcatctgcac
     4321 aagcgaggtg accgattctt cttcacgctg cttatggctg cgcgaatccc gtctcagcac
     4381 acgttttgcc tcgcgacgag ctttgatcac tgctttttgt cttcccatgg agagcacctt
     4441 gagttgtttg tattcatcac acgcgccgtt ggcagcgcga ttatgcgcac gaacatcaga
     4501 gggttggctt ccttgtaagc catagtttgc ttttggataa aatgccgaaa aacggctacg
     4561 cacaccgttt gcggcgtcgg taacacgagt aaagagagga agtgagaaaa atacagagtg
     4621 acgaagttgc taccggagga gaaatctccg caagtggtgg agcgttgctt aacaaaacat
     4681 tgtccagaac aggacgttac catccgcgat ctccatagtg actgactatc actgccggga
     4741 acttccgctg ctacttaata attacaacag atctcgcatt cattgcaaca atatattcac
     4801 ttatatttaa ctataaaaca ccatttcagt gacattagtt tctactggaa agatgacaga
     4861 gtgatgacag tgatgaaaaa agctgtgtgc tttcagcagg attcgc
//
LOCUS       DAWDRC010000284         4891 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7506_length_4891_cov_2.427213, whole genome shotgun sequence.
ACCESSION   DAWDRC010000284 DAWDRC010000000
VERSION     DAWDRC010000284.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7506_length_4891_cov_2.427213"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    629..638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2285..2294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1142 a         1098 c         1345 g         1286 t
ORIGIN      
        1 atggcctggg acgcgccggt ttttctatga aagccttcac catgcgcctg atgcatatgg
       61 gaaaagaggt ttacttcctg acggaaacca ttacgccgaa ctttgggcct ggcgatctgt
      121 ttatcgtttc ttccgcttcc ggtgagacag cacaactggt ggcactggcg aaaaaagcac
      181 gtcagcttgg cggcgctgtg gcggtgttaa ccacgaatcg acacgccact atcaccgagt
      241 ttgtcgatgt catcgtgcaa attaatgccc cttccaaaaa tcaaaaagat tctgttttcc
      301 gctcggcaca gccgatggcg tcgttgtatg agcaagcttt gctggtgatt gccgatgcat
      361 tagtgatgaa gatggctgct gaaagcggtg cgccggaaag tgaactgttt aagcgccatg
      421 ccaatctgga atagtcgtcg ccgttatttg ctcgataccc tacaggagaa taaaaatgag
      481 caaaaaaata acatccttgt ttaacttcaa gaagttgcag aaattttctt ctgctctgat
      541 gttaccaatc gccattcttc cggcggcggg tttgatttta gccatcggga tttcatttaa
      601 cattgagctg ctggcgaatt gcggcggcnn nnnnnnnntg attttcgcca accttccttt
      661 gctgttttgt gtcggtattg tgattggcct gacaggtgat ggttcttcat cactttcagc
      721 gattatcagt tacatgatca tgaatgccac gatgggaacg caacttggca ttaccgcgga
      781 gtccgttgca gccagtaacg gtgactatgc gatgatcctc ggggtaccga cgttacaaac
      841 gggagtgttt ggcggtttga ttgtcgccat ctttgtttat ctgctctata cccgcttcca
      901 caatattgaa ttgcctcagt ttcttggttt cttcggtgga aaacgctttg taccgattat
      961 tacggcggtg gcggcaatta ttgtcggcta tttattgccg tggctgtgga taccggtaca
     1021 aaaaggtctg gcgtcgcttt caacgctggt gaccagtggg cataacgcga acttcgcggc
     1081 ctttatttac gccgttggcg aacgttcgct gattcctttt ggcctgcatc atatctggaa
     1141 cgtgccgttt tattacaact ttggcgacta catgaccaaa tccgggcaac tggtgacggg
     1201 cgatattccg gtgttctttg cccaattgcg cgacggtgtt ccgttgacgg cggggctgtt
     1261 tatgacaggt cgtttcccga tcatgatgtt tgcgttacct gccgccgcgt tggcgatgta
     1321 tcaggaagca aaaccggaac gtaaggcgct gattaaaggc cttttactct ccggtgcgct
     1381 gaccacattt gttaccggga ttacggagcc gctggaatat gcatttttgt ttgcagctcc
     1441 ggttctctac atcattcaca tcttcttata tgccacttca tttgtgctga tgaacatcct
     1501 gaatgtgcac attggtcatc cttttgctgg cggtcttatt gatttcgtac tgaatggcgt
     1561 tatgccaaac cgcacgccgt ggtatctggt gttcctggca ggcgcgggct atgcagcgat
     1621 ttactacacc gtatttcggg tactgatccg caagttgaat ctgaaaacac cgggacgtga
     1681 ggatgaagag attgccgttg caaccacatt aacgcgcgaa gaacgtccat accaaatcat
     1741 cgaagcagta ggtggtttcg acaatatcga agatgtggat gcctgcgcaa cacgcctgcg
     1801 gctggcgctg gtagacgata agaaagtgaa tgaaaaacgg ctgaaggagt tgggggctgc
     1861 gggtttagtg aaactggggg atggcggtgt acaggtgata tttggcggca aatcgcaaat
     1921 cctgcgcgac gaaatcaaaa ccgtaatgag cagaccgcgc ccgacagaaa tgacagtggc
     1981 ctgtgctaac taattcgaga ttagcgggct ggtttcagcc cgccagacag agagagctga
     2041 agtatgaatt cacaattact ggctaacgcc atccggatgt taagtgttga tgcgatccag
     2101 aaagcgaact ccggtcatcc gggcgcgccg atggggatgg cggatattgc cgaagtggtc
     2161 tggcgtcgtc acctgcgtca taacccaaaa aatccgcagt ggtttaaccg cgaccgctat
     2221 gttcagtcga acggtcacgg ctccatgttg atttacgctt tgctgcatct tacaggctac
     2281 gaccnnnnnn nnnnccgttg gggcagggcg tagctaatgc ggtggggatg gcgattgcgg
     2341 agaaggcgct ggcggcagaa tttaacaaac cgggctttaa tattgttgat catcacacat
     2401 ggctattttt gggtgatggc tgcctgatgg aagggatctc ccacgaggcc tgtggtcttg
     2461 caggaacgct gaagctcggc aatctgattg ctatctggga cgataacggt atttcgatcg
     2521 atggtcacgt agaaggctgg ttcgctgaag atacggctgc ccgttttcgt gcctacggct
     2581 ggcatgtcat tgaaggcgtt gatggtcacg atccagaagc ggtagatgct gcggtgcgtg
     2641 aagcgaaaag cgtcacggat aagcccagtc tgctgtgctg caaaacgatt atcggttttg
     2701 gttcgccaaa taaagccaac agccacgatt gccacggctc ggcgctgggt gcagatgaag
     2761 tggcgctggt gcgtgaacgt ctgcaatggc cgtatgcccc tttcgaaatt cctggtgaaa
     2821 tttatgccga atgggatgcg accgagaaag gcgcgcaggt tcaacaggaa tgggatgcac
     2881 tgtttgccga ctacgcgaag cagtggccgg aactggcggc ggagtttact cgtcgcatga
     2941 agggtgattt gcctgctggc tgggccgaga atatgcagaa atatgtgcac gatctgcaat
     3001 cccatccggc ggcgctggca acccgtcagg tcagccagaa atgcctcaat cacttcgcgg
     3061 atatgctgcc ggaactgatg ggcggctcgg cggacttgtc gccctctaac ctgactcgcc
     3121 atcagaaatc ggttgatttc accggagaaa atccggcggg gaattacatc tcctatggcg
     3181 tgcgtgaatt tggtatgtcg gccatcatga acggtcttgc gctgcatggt gggtttatcc
     3241 cttacggcgg tactttcctg atgtttatgg aatatgcgcg taatgcactg cgcatggcgg
     3301 cgctgatgaa gatccgctcg gtgtttgttt atacccacga taccatcggc ctgggcgaag
     3361 acggcccgac acatcagccc gttgagcaac tggcgtcgct gcgtctgacg ccgaatatgg
     3421 aaacctggcg cggttgcgat caggtggaag tggcggtggc ctggcaacag gcgattgagc
     3481 gcaaagatgg cccaacgtcg ttggtattaa cgcgccaacc tctggcgcaa cagccgcgca
     3541 ccgctgcaca gttggcagag attgcgcgtg gtggttatgt actgagtgat tgtgacggtc
     3601 agcctgagat gatcctcatt tctgcgggtt cagagattga gttagtggtc agcgccgcaa
     3661 aagcgttaac ggaagaaggg cgtaaagttc gcgtggtttc tatgccgtgt accgagcgtt
     3721 tcgataatca ggatgcggcg tacaaagaat cggtattgcc aaaagctgta cgcaaacgtc
     3781 tggcggtaga agccagcatc gccggattct gggaacgcta cgtgggcctg gacggtaaag
     3841 tgatcggcat gacgagcttt ggtgagtctg ctccggcgaa tgtgttgttc aaacactttg
     3901 gttttacgcc ggaaaatgtc ctggcgcagg cgcgtgagct actgaacagc taaacctgca
     3961 caaggggtga aaggacgacc ttttacccct tgaaacggga gccgagaaac tcttcacggg
     4021 tgcggtattt ctcgtaatgc gccttgtttt tatacataaa caacagcacc agcatatcaa
     4081 taacgcccat tgctggaacg cgagtggagt cgaaggcttc aaagaagatt ttatcgctga
     4141 ccgcggtatt gagttgatgg gtggagagtt ttgataacgg agatttaata tagttagtga
     4201 tacaaacgga tttggcaccg cgctctttca ccactttaaa gatatcgacc attaagttgg
     4261 tgctgccaga gttagaaata ccaatcgcca ggtcacgttt ccccatagaa tatgccgcat
     4321 taatttgcat ttccgggctg acgtgaacaa cggtttttaa tccgagttgg ctgaatttat
     4381 aagcaccata aacggccaga gggtttgaat ttccggcagc aaaaaagaaa atagtatcgc
     4441 aattagcaag tagtttgaga atggactcaa gcacttcggc attaaaaaat tgcgttgatt
     4501 gtattaaatc ttcggcctgt ttttttaata aaaatgtggc cagcgacgtt atatcggtaa
     4561 aatcggtatt atcaggatag ctattgtcag gattaatgac ttcttttgct aatgcaattt
     4621 ttaaatggta ataaccctga taacctaatt tcttacaggt gcgtacgata gtcgcttcac
     4681 tggcatggct tttttctgat agttcagtga cactatagtt cacgacttca tgtgcgtggc
     4741 taagaatata gtcagcgacc tttttttccg cctgatgcag tgtagggtaa ttgctttgaa
     4801 tcagactttt tacggttatg ttattcataa ttagtaagtt tcattctgac agaaagtgct
     4861 gattataaca tagttttcag gcgtggatag g
//
LOCUS       DAWDRC010000285         4887 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7513_length_4887_cov_2.098096, whole genome shotgun sequence.
ACCESSION   DAWDRC010000285 DAWDRC010000000
VERSION     DAWDRC010000285.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7513_length_4887_cov_2.098096"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1141 a         1418 c         1277 g         1051 t
ORIGIN      
        1 cgacgccaga ggatactcgg gaaaatgagc ttcacttccc tgtttaatat tcagagatga
       61 catggggtat aacctgaaaa tgagtccaat aagagtaaat ctccacggcc cccactctac
      121 cagttggtga gatttatccg aagctgaagt ttgtaaatta ctcccactga atgcaacatt
      181 cactctattt cagagagata cccaccaaac ttataaatta aacaaatgat aaacccataa
      241 ttattttctg taattatggg tttatagata atataaattt acttatttag cgtcgcacga
      301 ataatcggcc caattttttc aaacgccacc ggagagataa tatccacatg ctcacaatcc
      361 tgacgataga tatccagctc agcaatccac ggtgcccagg cctgttcagg agtcatacct
      421 tcctgaagcg tgcgttcggc aacaaatagc gtcgctttac catcaaacgg tacgctatga
      481 gctgtcgtca gcaggcgcac agcatcagcg tagttgcctt caatggtggt aaacaactcc
      541 gttgaggtac ttccctgctg tgccgccagg aaggcttcgc gctcgcggtt aatctccgcc
      601 agcacttccg gatccagacc attggcttct ttttcctgcc agttttgcgt ttctggcggc
      661 caggtatcca gcaagccaag aaatgccacc tgttcgccac gggcacgcag tcgcgccgca
      721 atgccctgcg ccagcgtacc gccaagtgaa taacccagca ggtaatatgg gccgtgcggt
      781 tgttgttcaa gtaaggttgc cagatgcgct tcgcagactt catccaggtt tgccgccgtc
      841 tgcatggggc catgagggcg cggcgactga atgccgataa tcgaccattg tggatcgaga
      901 taacgcgaga gcacgctgaa ctgccaggca aaaccggatg ccggatggaa acaaaacagc
      961 gtcgggccat taccttcacg caacggcaga atggtttcga atcccatgcg ctgggtgctg
     1021 tcttcttcac catcaataat cgttgccagt ttggcgacag ttgacgcgac catcacctgc
     1081 cccggcgtca cctgacgagc aaactgtcga cttaactgcg ctgccagttt cattgccagt
     1141 agcgaatgac cgccaagcgc aaagaaatca gcatcggcat cctgcacgtc acaacccagc
     1201 aacgacgaga atgccgcggc gataatcgtt tcactgcccg ctttcggcac acgccccggc
     1261 gtttgtgcct tcagttcagg caacggtaag gctttgcgat ccagcttgcc gttggcgcta
     1321 agtggtaact gtggaagttg cagcagaacc accggtacca tatgtggtgg caatgtttct
     1381 cgaagctgcg cctgtaatgc gctggtatcc aacggcagac ctgattgcga caccagatag
     1441 cccaccaact gacgcgcatc accaccagtg gctgccgcct ggttaatcac acaggcgtgg
     1501 gtaacggctt gttcgacatc cggcagcgcc tgcatcacgc gatcaatttc gcccagttcg
     1561 atacgctgcc cgcgaatttt tagctgatcg tcactgcgcc cgaggtactc caccgcgccg
     1621 ttatccagcc agcgggctac atcgccggta cggtacatcc gttcaccagg ggcaaaagga
     1681 tcggcaataa agcggctggc ggtcagatcg ggtcgtccaa gatacccctg cgccagttga
     1741 ataccggtga gatagagatc tcccgccaca cccggcggca ccggatgcat catcgcatcg
     1801 agaatgcgca ggcccgtatt ccacaccgga taaccgatcg gcacactgct gccgcgcacc
     1861 tctgccagtt cctcgccaaa agccggatac caactgacat ctaccgccgc ttccgtcggg
     1921 ccatatagat tatgcaacgg cgcgccggtt aactgttgcc attcgcggca taaatcagcc
     1981 ggtaaggctt caccactaca gaaaacctgt ttcaacgtcg cgcaactctg gcgagcggtt
     2041 tgcggcgtca gcgatgcaac aaatgccgcc agcatcgacg gcacaaagtg cgtggtcgtt
     2101 acgccataat cagcaaaaaa atgctgcata gcgagcgggt cgcggtgcgc ttccggttca
     2161 gccatcacca gtttcgcccc ggcaataaac ggccagaaaa actcccacac cgagacatca
     2221 aaactgcacg gcgttttttg ggcaacaaca tcttcgcctg taagcgggta atggttttgc
     2281 atccatagca ggcggttaac gatagcctgc tgaccgacca ttaccccttt cggtctgccg
     2341 gtggagccag aggtaaagat gatataagcc gtgtggtgcg gttgtgaaag ctgcagcggc
     2401 gcactgccct gcggtgtcag cggggcgtta tagcaaaggc tggttaaatt ggggatatcg
     2461 ctaaagcgcg gcagttgatc gtcagtggtg attaacagcg acggacgcgc atcttccagc
     2521 atcattttca ggcgatcgtc cggatagccg gtatccaacg gtagccaggc cgcaccagct
     2581 tcaactatcg catgtagcgc cagggtcaaa aagaccgagc gcggtagagc caccgccacg
     2641 ctgtcgcccg gtttaacgcc gcgctcacgc agcagattcg ccagcgccac cacctgctcg
     2701 cgcatttccc gatagctgaa ctggtaacgc gcatctgcca gcgccggagc atccggtgtt
     2761 tttgccgctt gttctgccac cagcgcgctc agcgtggttt ctggaatctc aacctgagtg
     2821 gcgttgatct gcgccagctg cgcatactca cctggcagca taatatcgac atcgccgcac
     2881 aacagcgacg gattcgcagc gaactgcgca atcagcattt tcaggcgttc agcgtgttga
     2941 attaacgttg gctcatcgta acgctgttta ttggcgagga tttcaatact caaatcaccg
     3001 tgttcatccg ggaacagggc cagttcaagg tcattaaccg gaccggttgc cagggtatgg
     3061 gtttgcgcct gaacaccagg aatatccagt tggtaatcaa ataccttgat attgagtacc
     3121 ggaccgaaca gcggttcctc tcctgccgct cgcccgctgt cacggacaat ttgttcggca
     3181 tcgtaacgtt gatggcgacg catttttttc agttgagctg ccagtcgggt tgccagttgc
     3241 ggcagcgttt cttgcgccgc aatgtgaata cccaatggca aaacgttgag cacgggtccg
     3301 gtagccgtca gcgccgccga gcccagtcga cgcataaaga taaatccggc agcgtagtcc
     3361 atgcggttgc acaatcgccc cagccacaag gctgccaacg caagtgctaa atcggtacgc
     3421 tgcacacctg aaagttgcgt agccagctgg cggaattccc cgttggtaaa ttccagtttc
     3481 aggcgcaaaa tatctgccga agcgctgcgc cccggtaaag gtgccggaga aagtgacgcg
     3541 ggcggtggta gttgacgacg ctgttccgcc cagaatgccg catcacgctg ccaggcttca
     3601 ctttcgcggt actgctgata ctcttccact acatcagcga aaggcgtaaa cggcgaagca
     3661 ggcgttggtt cgccacgcag caatgcgcag taaatgttgg cgatctggcg tgtaatggcc
     3721 gggaaactga agccatcgac cagcaaatgg tgataacgct gataccagta ccagcggtta
     3781 tccgccacct gtatcagttg atgaaagacc agtggtttac cactatcgac gcgcagatct
     3841 tgttgcaaat ccgcctgcat taatgcccgc gcagtaccgt gcggatcaat gttggttcgc
     3901 aggtcgataa tttctggcag ttcgaacgtc tgcgcatcat cgacccactg ccagacttcg
     3961 ccgttatctt ccgtaaaacg catccgcaac gtatctgctt gcgctagtcc ggcaaccacc
     4021 gcgcgggcca gtagtggcgc atcaacctct ccggttaact caacgtaatg cgccacgctc
     4081 caggcggagg gtaaatccga cagtttttct gccatccaga tgccgggctg tgcggcgacc
     4141 aaaggtaaat gctggctcat tgtgcctcct gcaactgggt gaaattcgcc ggtgtcagag
     4201 tacgccagtg ggcttccagc cactgctggc aggacgcctg tgactgcggc tgacacacaa
     4261 cgtcccagcc tgccggtaag gcgcattgtt gcggccacag actgaattgc ccctgcgcat
     4321 tgcgcaaaat gtaaaacgct ccctgtggat catcgaaggg attactgaat gccatattca
     4381 actcctgtca tggaaaagtg gttgccagag gtcgattagc ccctgcatca agccaccgcg
     4441 ccaacaaagc gcatcatgtc cgccgtcaac ctgacgccag aaaatggatt cttttagggg
     4501 gtgtaattgc gcatacagcg cctgattggc ctgcatgatc atcggctcgc gaacacccgc
     4561 ttccagcaca atgcgcagac cttcggcgct aacttcacca gtatttagct gttcaagtaa
     4621 catgccctct tggtgcccgc cccgatgcgg ccaccagtac gaacctgact ggcttaatac
     4681 acagccaaag cgttcaggcc agtgcagtcc ggcatacagc gcggaaagcc caccaaaact
     4741 ctgtccggcg accacggtgc gatcggcgcg atcgctaaaa ggggcaatag ctttcacctg
     4801 gggtaataac tcttgctgta ctgccagcca gaaatccgca ttacacggca gttcgtgggc
     4861 gcggtgcgtg gtgtcgatag cgtcgat
//
LOCUS       DAWDRC010000286         4884 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7522_length_4884_cov_2.227998, whole genome shotgun sequence.
ACCESSION   DAWDRC010000286 DAWDRC010000000
VERSION     DAWDRC010000286.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7522_length_4884_cov_2.227998"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1178 a         1323 c         1298 g         1085 t
ORIGIN      
        1 tacccacacc agctctttct ggctggcctg tttacgcaga aacgctttgt ataaatcttc
       61 ggtaatcgca ctggaacata ccagcagctg gcaacttaac gttgacatta ccgccgccag
      121 aatcgccgac agcaaaatcc cggcaatcca cgggttaaac agaatttgcg ccagttcgat
      181 aaacacgcgt tcagcgttct ggtttaccgc acctgctacc gccgggtgct cgttaaagta
      241 agcgatacca aagaagccga cagccaccgc ccctgccagg cagaggatca tccaggtcat
      301 actgatacga cgcgcatgga caatgctgtg gtgagaatct gccgccataa aacgcgccag
      361 gatgtgcggc tgcccgaagt aacccagccc ccaacccatc agcgagataa tggcgacaaa
      421 attcaaccct ttgagcatat ccacgttttc gatgctcttt tgtttgatta cttccagcga
      481 gtcgccaaac ccaccgacgc taatgatcac gataaccggc gtcaggatca gggcaaaaat
      541 catcagactg gcctgtacag tgtcagtcca gctcaccgcg aggaaaccgc caataaaggt
      601 gtaaaggatc gtcgccgcag cccccgccca cagagccgtt tcgtagctca tgccaaaggt
      661 actttcaaac agacgcgcgc ctgccacaat gcctgaagcg caataaatgg tgaagaacag
      721 caaaataacc agcgcggaga taatgcgcaa aatgcggctt ttatcttcaa agcgcccggt
      781 gaaataatcc ggcaatgtta aggcgttatt gttgtattcg gtatgcacac gcaaccgccc
      841 ggccaccagc ttccagttga tccacgcgcc taatgtcaga ccaatggcga tccagctttc
      901 ggaaatcccg gaaagaaaaa cagcgcctgg caaccccatt aacagccagc cgctcatatc
      961 cgacgcaccc gccgataatg ccgtcacgaa tggcccaaga ctacgaccgc ccagaatata
     1021 gtcgtcgaag tttttcgttg atcgccaggc gataaaccca atcaatatca tgccaaagat
     1081 atagacacaa aatgtcacca acatcggtgt gctaatagcc atctaaagtc tccaaaaaat
     1141 tattatcggc aatgtcgaaa cttgccgtta tatctgccac cggaacgggg taacagagtt
     1201 tatgttttac cagggcgacc gtatcctgcc ggaagcgctg gttattcaca atcgatttaa
     1261 cacaccattt acattaaatt ttagtgctca gcgacactat ttttcatcag gttgcactct
     1321 ctcacatttt ttgcggttgc acctttcaaa aatgttaact gccgcagaga aaaagtctga
     1381 gttatttttt taatccctgt catatcgatt tcgtttatta acatttcatt catttttaag
     1441 cttgctacgc aggtcacatt taacacggtt gcacaaagtt gcaacatcat ggatatttca
     1501 cgataacgtt aagttgcacc tttcagaaca acaggagtaa tggcatggga accaccacca
     1561 tgggggttaa gctggacgac gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg
     1621 atcgcacacc acactggtta attaaacagg cgattttttc ttatcttgaa caactggaaa
     1681 acagcgatac tctcccggag ctacctgcgc tgctttctgg cgcggccaat gagagcgatg
     1741 aagcaccgac tccggcagag gaaccacacc agcctttcct cgactttgct ggaacaagct
     1801 cgcctgccgc agccagttgc tgaacaggcg cacaaactgg cgtatcaact ggccgataaa
     1861 ctgcgtaatc aaaaaaacgc cagtggtcgc gcaggtatgg ttcaggggtt attgcaggag
     1921 ttttcgttgt catcgcagga aggcgtggcg ctgatgtgtc tggcggaagc gttgttgcgt
     1981 attcccgaca aagccacccg tgacgcgtta attcgcgaca aaatcagtaa cggtaactgg
     2041 cagtcacaca ttggtcgtag cccgtccctg tttgttaatg ccgctacctg ggggctgctg
     2101 tttaccggca aactggtttc cacccataac gaagccagcc tctcccgctc gctgaaccgc
     2161 attatcggta agagcggtga accgctgatc cgcaaaggtg tggatatggc gatgcgcctg
     2221 atgggcgagc agttcgtcac gggcgaaacc atcgcggaag cgttagccaa tgcccgcaaa
     2281 ctggaagaga aaggtttccg ttactcttac gatatgctgg gcgaagccgc actgactgcc
     2341 gcagatgcac aagcgtatat ggtttcctat cagcaggcga ttcacgccat tggtaaagcg
     2401 tctaacggtc gtggcatcta tgaaggtccg ggcatttcaa tcaagctgtc ggcgctacat
     2461 ccgcgttata gccgcgccca gtatgaccgg gtaatggaag agctttaccc gcgtctgaaa
     2521 tcactcaccc tgctggcgcg tcagtacgat attggtatta acatcgatgc cgaagaggcc
     2581 gatcgcctgg agatctccct cgatctgctg gaaaaactct gtttcgagcc tgaactggca
     2641 ggctggaacg gcatcggttt tgttattcag gcttatcaaa aacgctgccc gttggtgatc
     2701 gattacctga ttgatctcgc cacccgcagc cgtcgccgtc tgatgattcg cctggtgaaa
     2761 ggcgcgtact gggatagtga aatcaagcgt gcacagatgg acggccttga aggttatccg
     2821 gtttataccc gcaaggtgta taccgacgtt tcttatcttg cctgtgcgaa aaaactgctg
     2881 gcggtgccaa atctaatcta cccgcagttc gcgacgcaca acgcccatac gctggcggca
     2941 atttatcaac tggcgggtca aaactactac ccgggtcagt acgagttcca gtgcctgcat
     3001 ggtatgggcg agccactgta tgagcaggtc accgggaaag ttgccgacgg caaacttaac
     3061 cgtccgtgtc gtatttatgc tccggtcggt acacatgaaa cgctgctggc atatctggtg
     3121 cgccgcctgc tggaaaacgg tgctaacacc tcgtttgtta accgtattgc cgacacctct
     3181 ttgccactgg atgaactggt cgccgatcca gtcactgctg tagaaaaact ggcgcaacag
     3241 gaagggcaaa ctggattacc gcatccgaaa attcccctgc cgcgcgatct ttacggtcac
     3301 gggcgcgaca actcggcagg gctggatctc gctaacgaac accgtctggc ctcgctctct
     3361 tcagccctgc tcaatagtgc actgcaaaaa tggcaggcct tgccaatgct ggaacaatcg
     3421 gtcgcggctg gtgagatgtc gcccgttatt aaccctgcgg aaccgaaaga tattgtgggc
     3481 tatgtgcgtg aagccacgcc gcgtgaagta gaacaggcgc tggaaagtgc ggttaataac
     3541 gcgccaatct ggtttgccac tcctccggct gaacgcgcgg cgattttgca tcgcgctgcc
     3601 gtgctaatgg aaagccagat gcagcaactg attggtattc tggtgcgtga agccggaaaa
     3661 accttcagta acgccattgc cgaagtgcgc gaagcggtcg attttctcca ctactatgcc
     3721 ggacaggtgc gggatgattt cgctaacgaa acccaccgtc cattagggcc tgtggtatgt
     3781 atcagtccgt ggaacttccc gctggctatt ttcaccgggc agatcgctgc cgcactggcg
     3841 gcaggtaaca gcgtgctggc aaaaccggca gaacaaacgc cgctgattgc cgcgcaaggg
     3901 atcgccattt tgctggaagc gggtgtaccg ccaggcgtgg tgcaattgct gccaggtcgg
     3961 ggtgaaaccg tgggcgcgca actgacgggt gatgaacgcg tgcgcggggt gatgtttacc
     4021 ggttcaaccg aagtcgctac gttactgcaa cgcaatatcg ccagccgcct ggacgctcag
     4081 ggtcgcccaa ttccgctcat tgcggaaacc ggcggcatga acgcgatgat tgtcgattct
     4141 tcagcactga ccgaacaagt cgtaatagat gtgctggcct cggcgttcga cagtgcaggt
     4201 cagcgttgtt cggcactgcg cgtgctgtgc ctgcaagatg agattgccgg ccacacgctg
     4261 aaaatgctgc gcggcgcaat ggccgaatgc cggatgggta atccgggtcg cctgaccacc
     4321 gatatcggtc cggtgattga tagcgaagcg aaagccaata tcgagcgtca tattcagacc
     4381 atgcgtagta aaggccgtca ggtgttccag gcggtgcggg aaaacagcga agatgcccgt
     4441 gaatggcaaa gcggcacctt tgtcgctccg acgctgatcg aactggatga ctttgccgaa
     4501 ttacaaaaag aggtctttgg tccggtgttg catgtggtgc gttacaaccg taaccagctg
     4561 ccggcgctga tcgagcagat taacgcttcc ggttatggtc tgacgcttgg cgttcatacg
     4621 cgtattgatg aaaccattgc ccaggtcact ggctcggccc aggtcggtaa tctgtacgtt
     4681 aaccgtaata tggtgggcgc ggtggttggg gtgcaaccgt tcggcggcga agggttgtcc
     4741 ggtaccgggc cgaaagcagg cggtccgctc tatctctacc gtctgctggc gaatcgcccg
     4801 gaaagtgcgc tggcggtgac gctcgcgcgt caggatgcgg agtatccggt cgatgcgcag
     4861 ttgaaagccg cattgactca gccg
//
LOCUS       DAWDRC010000287         4884 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7524_length_4884_cov_1.809691, whole genome shotgun sequence.
ACCESSION   DAWDRC010000287 DAWDRC010000000
VERSION     DAWDRC010000287.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7524_length_4884_cov_1.809691"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3150..3159
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4279..4288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1156 a         1256 c         1201 g         1251 t
ORIGIN      
        1 ttcacatcgg tgagctgaat atgcacttgc gcggtcatat ccagccgcag taaaccattg
       61 ggattgggta cttcaaaacg ggcgtaatag aaaatagcgt cgttaatctt ttccggcgtc
      121 ggtagcacat ccttgatttg cccctcgtag cgcgtcagtg gatcgccaag caccgtaaac
      181 caggcttttt gccccggctt caggtggatt acatccgctt cagaaacctg cgcttttacc
      241 agcatggtgc tcatatctgc cagcgtcaga atgttcggtg cttgttgtgc ggcaatcacc
      301 gtctggcctg gcagagtggt gatttgcgtg acttccccgg ccatcggggc aacgatgcgg
      361 gtgtaatcga gattggtttt agccgtatcg agagaggcct gattgcgctt gatttgcgcg
      421 tcaatggtgc caatttgcgc ctgtttcaca gccatctccg tcgcggcgtt atcgagatcc
      481 tgctgtgaaa ccgcttgcgt ttgtgcaaga cgttgctgac gggaatacgt cacccgcgcc
      541 agtttcagct ccgcttccgc ctgctgccgc tgcgcgcgta gctccatcag cgttgcttcc
      601 acctccttga tctggttttc agcctgttca ggatcaataa cccctaaaag ctggtctttt
      661 tttactttat cgccaatcgc caccgacagc gttttcaact gaccgctgac ctgcgcgccc
      721 acgtcaacct tacgcagcgc gtccagcttt ccggtcgcca gcacgctttg ctgtaaatca
      781 ccggggcgca caatcagcgt ctgataagtc ggcacgggtg cgttaagaat tctccataac
      841 gtaatcagcc cggcgatgac tatcaccatc gcaataacgt aacgcttctt cacggttttc
      901 cgctttttca taaatattct gaatactcca tatgccaatc cgggtcatta acttcaacga
      961 aatatcaaca aaaccctaaa atttacgtta ataatcacac tattgatgtt tggttaagat
     1021 acgcaatggt taaatcgacg tcatgtataa ccattgattt catgaatatg tcgcagctaa
     1081 ctgaacggac ctttacgcca tctgaatctc tcagcagcct gtcacttttt cttagtctgg
     1141 cacgaggaca gtgtcggccg ggtaaattct ggcatcgccg tagttttcgc cagaaatttt
     1201 tgctgcgctc gttgattatg ccgcgtttaa gcgttgagtg gatgaacgaa ctttcccact
     1261 ggcctaatct caatgtgttg ttaacgcgcc agccgcgact gcctgtacgt ctacatcgcc
     1321 cttaccttgc ggcgaatctt agccgtaagc aattgctgga ggcgttacgt taccattatg
     1381 cgttactccg cgggtgtatg tcggcggaag aattcagctt atatttgaat acccccgggc
     1441 tgcaactggc gaagctggaa ggcaaaaacg gcgagcagtt cacgcttgag ctgaccatga
     1501 tgatctcaat ggataaagaa ggtgacagca ccatcctgtt ccgcaacagc gaaggtattc
     1561 ctctggcaga gctcactttt accctgtgtg aatatcaggg gaaaaggacg atgtttattg
     1621 gcggactgca aggcgcaaaa tgggaaattc cacaccagga aatccagaat gcgacgaaag
     1681 cctgccacgg gctatttccc aaacgcctcg tgatggaagc ggcctgtctg tttgcccaac
     1741 gtttgcaggt agagcagatt attgccgtca gcaatgaaac gcatatttac cgcagcctgc
     1801 gttatcgcga taaagaaggc aagatccatg ccgattacaa cgctttctgg gaatcggttg
     1861 gcggcgtatg tgatgctgaa cgccattacc gccttccagc acagatagca cgaaaagaga
     1921 ttgccgaaat cgccagtaaa aaacgggctg aataccgtcg gcgctatgag atgctcgacg
     1981 ctattcagcc acaaatggcc acgatgtttt gtagttagtc ggcccgaccg cgcgccagcc
     2041 acagcacgcg tgagaacatt tttttcagca gcgtcggtac ggagtccaca ccacgacgtc
     2101 cggcctccat tgccacttca atggcaagat cgggtttgga agagcgatgg atcgctttcg
     2161 agataacttt gcgcagattc gtcggtatat tttccgggat ttgcgccacg cggtggaaca
     2221 catcggaaaa tccctggcga tacataaaat gttccatatc cagcgccggt aacgccgtta
     2281 aatgttctcg ttcggcttcc cgatcgttat tcaacaggct gcgtaccgta gcggcatatt
     2341 tcttccctgc ttcatcgcca tcgaccagta catgccattc aatccccatt cgccgggcaa
     2401 atttaaccag cggctttagc ccggactggg caaactcaat gaccttgatc ccttcggcat
     2461 cgaaatggtg tccacactgg cgcgccagtt cgttgataac ccaggtttcc gtttcccctt
     2521 ccaccaacag ccagcagcga gcaaacagcg atgacggacg gttaaaacga atgtgaaagg
     2581 atatgcgtcg gctatcttcg gtactcaagc cactcggtcc cagacgccag gcggcaacgc
     2641 gagaggactc acgtaccagt cggcaaacat gctctaccgg cgttaacgaa agcaactctc
     2701 ccgagttggt agtagcgatg cgctgcaatg gcagaagatt caacagatgc caggcaactg
     2761 aaagcataat ggggtgtaaa cgggtttctg gatcttcgat cagcaacaat ggacgggcgt
     2821 ctttatccag tcgcaatgtg ccttttgcct gcaacaaagt agcaaacagg ccgagcaaaa
     2881 taacccgata cgagcgccct ccaggtcggt caatcatccg gttgatgata tccagatagc
     2941 gccagcttcg ttgctcattg ctggctcgcc gccgcattaa acgatatcgc gcctgtccgg
     3001 ccccctgctc agagaaataa tgctcaagca gctgtaccat tgcggaaagt ccctgacgaa
     3061 tctgcccatc agagagattt tgcggatgtg aggataattc acgggcgagg aaatcgagct
     3121 ggcgcgcggt gacttccaca ttggggacan nnnnnnnnna tttggcaccg tgccgttacg
     3181 aatacggcgc ataaaacggg catcacgcaa gcgcagcacc ggcattaaac gcaccagatg
     3241 acgagcctga tcgttaatat cctcaacatc aatcggatgt ccgtctttat cgagaaaact
     3301 gcggagtgtc atcacgctgc cgtcttccgc actctcccct tccagacgat aaaaaatacg
     3361 gtgataacca tcggtgcatg gcgtccagca cgcttccagc ggccgatagc ggcgaacccg
     3421 atgtcggcct ggcagcgatt cgcggaaggt caaaataata tgcagatgat gttctcgccc
     3481 gttgatatct cccggcggga accagaaatc atcgcgctca aaatggtaga gatctgattc
     3541 tggcgatagc agcagggtta aggcgtccag caagctggat ttaccccacg cgttctcccc
     3601 aatcaggacg ttgttttgtt ccagcatcaa cgacaaacgg ttgataccgc gaaaacccac
     3661 aatttcaacg cgctcaagaa tcatatcccc tccagaaata tgtgacttat cctttacgct
     3721 ggcagtataa cgggaaaggc catgctaata cgtgatgaca tcacatttaa gcatggcaaa
     3781 tgtctgaaaa atgagcgaat aatcgcgtta tactccctct tgctgataag cctgcaaaaa
     3841 caacaaaatc aaataattag catgataaat tattttttat tacgcctaaa ggattaaatc
     3901 tttcgcttgc actacactgt caatcagaca aatgactata tggggcaacc cgctgttgcc
     3961 ttaaatcaaa tttactggat ttatttatct ggtgaatgac ttgccttcgt ttttaaaata
     4021 gccgcgcatc atttccgtcg tcactttttt acgacgaccc tataaaacga ccatattttt
     4081 cacagggtca atttttaatt gaggtggata tgttcagaaa attagcagct gaatgttttg
     4141 gtactttctg gcttgttttt ggcggctgtg gtagtgccgt actggccgca ggcttcccgg
     4201 aattaggcat tggttttgcc ggcgtggcgt tggcgttcgg tctgaccgtt ctgacaatgg
     4261 cctttgctgt tggtcatann nnnnnnnngg tcattttaac ccggcggtca ctattggttt
     4321 atgggctggc ggacgtttcc cggcaaaaga agtcgttggc tacgtaattg cccaggttgt
     4381 cggcggtatt gttgcggcgg cgctgctgta tttaattgcc agtggtaaaa cgggttttga
     4441 cgcggcagcc agcggttttg cttctaacgg ttatggcgag cattcaccag gcggttattc
     4501 catgctttcc gcgctggtag ttgaactggt attgagtgca ggtttcctgc tggtgatcca
     4561 cggcgcaact gacaaattcg ctcctgctgg ttttgcgcca attgccattg gtctggcctt
     4621 aaccctgatt cacttaatca gtattccggt gactaacact tccgttaacc cggcgcgcag
     4681 tactgcggtt gctatattcc agggcggctg ggcattagag caactgtggt tcttctgggt
     4741 ggtgccaatt gtcggcggta ttatcggtgg tctgatttac cggactctac tggaaaaacg
     4801 cgattaatta acgcagaagg cctggcgcaa tgtcaggcct ttttttaaga tgttgttata
     4861 tgttgattca tcatcttgtt gcgc
//
LOCUS       DAWDRC010000288         4869 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7547_length_4869_cov_2.216452, whole genome shotgun sequence.
ACCESSION   DAWDRC010000288 DAWDRC010000000
VERSION     DAWDRC010000288.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7547_length_4869_cov_2.216452"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3654..3663
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1215 a         1171 c         1363 g         1110 t
ORIGIN      
        1 gctgaaaggc aacagttata tgcagtggat gatcccaggg ctgcatctgg aactggggcc
       61 aaacagtgcc gaagtgaaag gcgagctggg ggtaaaagat ctcaatcttg atgccaccat
      121 caacgcgccg gggctggata acgcgctgcc ggggcttggc ggtaccgcga aagggctggt
      181 gaaagtacgc ggcacggtgg aagcgccaca actactggca gatatcaccg cgcgcggcct
      241 gcgctggcag gaactttccg tggcgcaggt tcgcgtggaa ggcgacatta aatccaccga
      301 tcagatcgcc gggaaactcg acgtacgcgt tgagcaaatt tcgcagccgg atgtaaatat
      361 caacctcgtc accctgaatg ccaaaggcag tgaaaagcag cacgagctac agttgcggat
      421 tcagggcgag cctgtctccg ggcagcttaa tctggcagga agttttgatc gcaaagaaga
      481 acgctggaag ggaactctta gcaatacccg cttccagacg ccggtcggcc cgtggtcgct
      541 gacccgcgat attgcgctgg attaccgcaa taaggagcaa aaaatcagca tcgggccaca
      601 ctgctggctt aacccgaatg cggaactgtg cgtgccgcaa actatcgatg cgggggccga
      661 agggcgtgcg gtggtgaatc tcaaccgctt cgacctggcc atgctgaaac catttatgcc
      721 agaaaccact caggccagcg gtatcttcac gggtaaagcg gacgttgcct gggacaccac
      781 gaaagagggg ctgccgcagg gcagtatcac cctttcgggg cgtaacgtgc aggtaacgca
      841 aaccgtcaac gatgcggcgc tgcccgtggc gtttcagacg ctgaatctga cggcggagtt
      901 gcgtaacaac cgtgccgaat tgggctggac catccgcctg accaataacg gccagtttga
      961 tggtcaggtg caggtgaccg atccgcaagg ccgccgtaat cttggtggca acgtcaatat
     1021 ccgtaacttc aaccttgcga tgataaaccc catctttacc cgtggggaaa aagcagcggg
     1081 gatggtgagt gccaacttgc gtctgggtgg tgatgtgcaa agtccgcagt tgtttggtca
     1141 gcttcaggtt acgggggtgg atattgacgg caactttatg ccgtttgata tgcaaccgag
     1201 ccagcttgcg gtcaacttta acggtatgcg ctcgacgctt gcaggtaccg tgcggaccca
     1261 gcagggtgaa atctacctga acggtgatgc cgactggagc cagattgaaa actggcgggc
     1321 gcgggtaaca gcgaagggca gtaaagtgcg tatcaccgtg ccgccgatgg tacgaatgga
     1381 tgtatcgcca gatgttgtat tcgaggctac accaaacctg tttaccctcg atggtcgcgt
     1441 ggatgttccg tgggcgcgca tcgtggtgca cgatctgccg gaaagcgcag taggcgtttc
     1501 cagcgatgtg gtgatgctta acgataacct gcaaccggaa gagccgaaaa cggcgtcgat
     1561 tccgattaac agtaacctga ctgtccacgt tggcaacaat gtgcgcattg acgcctttgg
     1621 cctgaaagcg cggctgacgg gcgatctcaa cgtcgttcag gacaaacaag ggctgggcct
     1681 gaacgggcag atcaacatcc ctgaagggcg cttccatgcc tatggtcagg atctgattgt
     1741 gcgtaagggt gagctactgt tctctggtcc gccagatcaa ccgtatctta atatagaagc
     1801 tattcgtaac ccggatgcta cagaagacga cgtcatcgcc ggagttcgcg tcactggtct
     1861 ggcggacgaa ccgaaagcgg agatcttctc tgacccggcg atgtcgcaac aagctgcatt
     1921 gtcttatttg ctacgtggac aagggctgga gagcgatcag agcgacagcg cggcgatgac
     1981 ctcgatgctg attggcctgg gggttgcgca aagtggccag attgtgggta aaatcggcga
     2041 gacgtttggc gtaagcaatt tagcgctcga caccgaggga gttggcgact cctcccaggt
     2101 ggtggtcagc ggctatgtat tgccaggcct gcaagtgaaa tacggcgtgg gtatatttga
     2161 ctctatagca acactcacgt tacgttatcg cctgatgcct aagctatatc tggaagccgt
     2221 gtctggtgta gaccaggcac tggatttgct ctatcagttc gagttttagc aatgcgaata
     2281 tttgtctacg gcagtttacg ccacaaacaa ggcaacagtc actggatgac caatgcccag
     2341 ttactgggcg atttcagtat cgataactac cagttgtata gcctgggcca ctatccaggc
     2401 gcagttccgg ggaacggaac agtacacggt gaagtttatc gtattgataa cgccacgctg
     2461 gccgaacttg atgccttgcg caccaggggc ggtgaatacg cgcgccagtt gattcagacg
     2521 ccgtacggga gtgcatggat gtacgtttat caacgacccg tcgatggatt aaagctaatt
     2581 gaaagcggcg actggttaga cagggataag taataataaa agcacgccac cttcgggtgg
     2641 cgttgtttta tgcgatgtat ctcgcgattt tttttgtaac tttcccatct tttgcttttc
     2701 tctccgggcc gctttccata tctgcaatcg cataaaaaac tctgctggcg ttaacaaatg
     2761 tacaggggta aaacggggac acgctttgaa cggtgagtat gagtgaggcc ggctaacttc
     2821 cagcaagtgg ctatgaacag caaaatggtg gtcgagttta gcttgttctt accgttcaag
     2881 cctttaaact gctgaaaata ataatagtgg agtagagagt ttataaattg cgggcataaa
     2941 aaaaccccta ctaaccgaag cccggcgttc agggttatta cgccagaaga acttatttat
     3001 tctttgcgcg ctcgaaggag gcaacgattt cagctttagc ggcttctgcg ttttcccaac
     3061 cttcaacttt aacccacttg cctttttcga ggtctttgta gtgctcgaag aagtgagcga
     3121 tttgcgcttt cagcagctct ggcagatcgt taacgtcttt aatgtgatcg tattctttgc
     3181 tcagcttggt gtgcggaaca gcaatcagtt tcgcatcttc accggcttcg tcggtcattt
     3241 tcagtacgcc aaccggacgg cagcggatca cagaacccgg ctgcagcggg tacggagtcg
     3301 ggaccagtac gtcaaccggg tcaccgtcca gagacagggt gtggttgatg taaccgtagt
     3361 tgcacggata gaacatcgcg gtggacatga agcggtcaac gaacagtgcg ccgctctctt
     3421 tgtcgatttc gtatttgatc ggatctgcgt tagccgggat ctcaataaca acgtagatgt
     3481 cttccggcag atctttaccc gcagggacgt tgagtaagct catgtctgtt tcctttaaaa
     3541 atatgtggca aacaagtgcc gagtattata gccaactcgc gccgaatgtc ttcgcttgtt
     3601 ttcgtcttca ttttcctttt tcgccagttt taagacggta tacagagcag gaannnnnnn
     3661 nnnggaaaat ccataaccgt agccgcattt ttcatagtga gatgaaagcg attacaaact
     3721 tgtgattaac gttttattta cttttttgaa gtgtgatgta acgcaatccg ttacataacg
     3781 aattgtctat agttttttcg cgaacatctt ttcaccaata ataattaccc cgacgaggac
     3841 aaccctatgt ggaaacgctt acttgtagtc tctgcagtct cggcagccat gtcgtctatg
     3901 gcgttggccg ctccattaac cgtaggattt tcgcaggtcg gatcggaatc cggctggcgc
     3961 gccgcagaaa ccaatgtggc gaaaagtgaa gccgaaaagc gcggaattac gttgaaaatt
     4021 gccgatggtc agcaaaagca ggaaaaccag attaaagcgg tacgttcctt cgtcgcgcaa
     4081 ggggtggatg cgatctttat cgctccagtg gtagcgaccg gttgggagcc ggtattaaaa
     4141 gaggcgaaag atgccgaaat cccggtcttc ttgcttgacc gttccatcga tgtgaaagac
     4201 aaatctctct atatgaccac tgtcaccgcc gacaacatcc tcgaaggcaa gttgatcggt
     4261 gactggctgg taaaagaagt gaatggcaaa ccatgcaacg tggtggagct gcagggcacc
     4321 gttggggcca gcgtcgccat tgaccgtaag aaaggctttg ccgaagccat aaagaatgcg
     4381 ccaaatatca aaattatccg ctcgcagtca ggtgacttca cccgcagcaa aggcaaagaa
     4441 gtgatggaga gctttatcaa agcggaaaac aacggcaaaa acatctgcat ggtttacgcc
     4501 cataacgatg acatggtgat tggtgcaatt caggcaatta aagaagcggg cctgaaaccg
     4561 ggcaaagata tcctgacagg ctctatcgac ggcgtgccgg acatctacaa agcgatgatt
     4621 gatggcgaag cgaacgccag cgttgaactg acgccgaaca tggcaggccc cgcttttgat
     4681 gcgctggaga aatataaaaa agacggcacc atgcctgaaa agctaaccct gaccaaatcc
     4741 accctttatc tgcctgatac cgcaaaagaa gagttagaga agaagaaaaa tatggggtat
     4801 tgagggttgc tatgcctgat gccgattcgt aggccggata aggcgctcgc gccgcatccg
     4861 gcggtggtg
//
LOCUS       DAWDRC010000289         4868 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7549_length_4868_cov_2.339705, whole genome shotgun sequence.
ACCESSION   DAWDRC010000289 DAWDRC010000000
VERSION     DAWDRC010000289.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7549_length_4868_cov_2.339705"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1234..1243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1284 a         1248 c         1224 g         1102 t
ORIGIN      
        1 cggcaggcac gtaaacggtc gaaattctct tcatcttcaa acaggttgac gatctgcatg
       61 atgcccactt cctggtgagt gttggcatca accgccgcca tggtgatgag gatatccacc
      121 ggatcgttat cttcgtggtt gaactccagc ggctttttca gcgtcaccag cgcaaaacca
      181 gttttcttaa ccccctcttc cgggcgtccg tgcggcattg ccaggccggg tgcgagcaca
      241 aagtacgggc cgtgctgttc aaccgcatcc agaatcgcct ggtagtaacg cggctctacc
      301 acatctgccg ctaccagcag atcaacgccg attttcactg cgtcctgcca tgtctctgct
      361 tccgcttgca ggcggataga tttattttca gccagcgaat cacgtaattt catggcgcgt
      421 ccttacttca cgtcctgcgg gaaatgctct ttgatcactt ccagcagttt cgggccaaaa
      481 tcagcaggag agagcatgtt gcgcacgcca accacatatt tgttgccggt cacggtgatt
      541 tcgcccgcaa tatgggtaga agcgatgatg atatccgcac cgctcaactc gcttttgtat
      601 tcgccaaccg cgcagctgtt taccgtgtgg tcgatgttcg actgggttaa aaactggtcc
      661 actttcattt tcatgatcat ggaactgcct tgtccgttac cacacacagc cagaatacgt
      721 acggtcataa tcaaaactcc ttattaagca gattgttctg ccagttgttt ttctgcatct
      781 tcttctgcac gcagtgcgcg gccagcgaag aacatataag ccagtgcaat gacaatgatg
      841 acggccataa aggcgatacc gatggagaag aagccctgca tcatcggcgg tgccagaatc
      901 gaccagtccg ccatgcccat ccaggcactc ataccggtga gtttcaccgc ccagacgcaa
      961 ccaaagattt cgatcatccc catcaccagg caaatcttca gcgccgcgcg ccagccgccg
     1021 aagtggttag cgaacacgcc gatagtggcg ttggagaaga acatcgggat aaagcctgga
     1081 ataatcagga ttgaagagcc acaggcgacc aggatgccaa ccgcaatcag ttgcccgatg
     1141 gtgccccaca taaaacccca gaccacggcg ttcggcgcga agctataaat agccgcacag
     1201 tcaatcgcca gaaccgcacc cgggatcagg cgcnnnnnnn nnncgttgaa tgcttcagag
     1261 agctccgcca caaacatgcg cacgccctga gtgatgatga agatcgccac cgcgaaggag
     1321 aaaccggttt gcaggatgta caccgtccag ttcactttgc ctgccatcgc ctgcacggtg
     1381 tcgataccaa aggagagcag aatggcacca aagaagatag tcatgacaat cgccgtggag
     1441 acgatgttgt cgtggaaaat attcagccag ccaggcagtt tgaggtcttc aacgctctcc
     1501 tcttttttgc cgaggaacgg tgcgacttta taagcaatcc atgatgcgaa ctgctgctgg
     1561 tgaccaatgg agaaaccaca gccgtcggtg acttcctgtg tcggcttgta catcatgttg
     1621 gaggtaatgc cccagtagag cgaaaccaga atcgcggtgc agataatggt cgtccacatg
     1681 gagtagccga agataaacag cgtaacggca atcagccccg cctgctggaa catgatgtga
     1741 ccagtcagca tgatggtacg aataccggta atgcgacgca gcagcacgta acagatgttc
     1801 agcgccagcg ctaacaacac cgcgtaaccc acccagctat aggcatcgcc catgcgttca
     1861 atggttgcca tcatcgacgc gtaagtatcg gaaattgcgc cgttaatgcc gtagacttcg
     1921 gacattttcg ccaccaccgg tttgaaggtg ctggtcagga tgccggaccc tgcctgcaac
     1981 aacatgaaac caattatggt tttaatcgta cctttaataa taacgctgac acttttgcgc
     2041 agtaggatgt aacccagaca ggtcacaata cccagcaaca acggggcatt ggtcatgacc
     2101 tggttaaaaa acacggtaaa gatgttgtag aggatctcca taacgatctc ctgaagaaga
     2161 ggtaaccggg tatttcgcac acccggaatg ttgtgctctc actctaattt tcaaaagtaa
     2221 tcacaacaag attatttatg attaaatgtg acgtcgcacg caaataattc cacataaaca
     2281 gaatgggatt cacataatga aatatttatt catttaaatt catttagtta attgcattac
     2341 acattcaatt taatctttct acactcaaaa tccataataa ctctcctgct aaaaagtcaa
     2401 aaaatggtca ttgccttcct gaaaaaatag tgtcaggatt aatcaaaacc aatcacatat
     2461 tgattcgaat tgaactatca cgaaggtaaa tggcgatgag taaagtgaaa agtatcaccc
     2521 gtgagtcctg gatcctgagc actttcccgg agtggggtag ctggctgaat gaagaaattg
     2581 agcaagagca ggtcgcacca ggcactttcg caatgtggtg gctcggctgt accgggatct
     2641 ggttgaaatc agaaggcggt gccaacgttt gtgttgattt ttggtgcggt actggcaaac
     2701 aaagccacgg taacccgtta atgaaacagg gtcaccagat gcagcgcatg gctggcgtga
     2761 aaaaactgca gccaaacctg cgcaccaccc cgtttgttct tgatccgttt gcgattcgcc
     2821 agatcgacgc ggtactggcg actcacgatc acaacgatca tatcgacgtt aacgtcgctg
     2881 ctgccgtgat gcagaactgt gctgatgacg taccgttcat tggaccgaaa acctgcgttg
     2941 atttgtggat tggctggggc gtaccgaaag agcgttgcat cgtggtcaaa ccgggcgatg
     3001 tcgtaaaagt gaaagacatt gaaattcatg cacttgatgc tttcgaccgc acggcactga
     3061 tcaccctgcc tgccgatcaa aaagcggctg gcgtactgcc ggacggcatg gacgatcgcg
     3121 cggtgaacta tctgttcaaa accccaggcg gtaccctgta tcacagcggc gactcccact
     3181 actctaacta ctatgcaaaa catggtaatg agcatcagat cgacgtggct ttaggatcgt
     3241 acggagagaa cccacgtggt attaccgaca aaatgaccag tgctgatatc ctgcgtatgg
     3301 gcgaagcgct gaatgcgaaa gtagtgatcc cgttccacca cgatatctgg tcaaacttcc
     3361 aggccgatcc gcaagagatc cgcgtgctgt gggagatgaa aaaagatcgc ctgaagtatg
     3421 gcttcaagcc gtttatctgg caggtcggcg gcaaatttac ctggccgctg gataaagaca
     3481 acttcgagta ccactatccg cgtggtttcg atgattgctt cactattgaa ccagatctgc
     3541 cgttcaagtc gttcctgtaa tctgtcgctt gtaccggata gcgtggagat gctcgcgcta
     3601 tccggcatag tatttcctgg ctatttcaaa tatcatctaa aaaaatcaaa ttttatcgga
     3661 atagctcatg actgaagcac aaagacatca aatcctcctg gaaatgctcg cacaattggg
     3721 ctttgtgacc gttgagaaag tcgttgagcg tctgggaatt tcgcctgcca ctgcacgacg
     3781 cgatatcaat aaacttgacg aaagcggcaa actgaaaaaa gtgcgcaatg gcgcagaagc
     3841 tattacccaa cagcgcccgc gctggacgcc gatgaatctg catcaagcgc agaatcacga
     3901 tgaaaaagta cgtattgcta aagcggcctc gcagctggtt aatccgggcg aaagcgtagt
     3961 catcaactgc ggctccaccg cgtttctgct tgggcgggaa atgtgtggca agccagtgca
     4021 aatcatcact aattatctac cgctggcaaa ttacctgatc gatcaggagc atgacagcgt
     4081 gatcattatg ggcggacagt acaacaaaag tcagtccatc actttaagcc cgcaaggcag
     4141 cgaaaacagt ctctatgccg ggcactggat gtttaccagc ggcaaaggac tgacagcaga
     4201 agggttgtat aaaaccgata tgctgacggc aatggcagag cagaagatgc tgagcgtggt
     4261 agggaaactg gtggtactgg ttgatagcag taagattggc gaacgcgcgg gaatgctttt
     4321 tagccgtgcc gatcaaatcg atatgctgat caccggcaaa aatgctaacc cggaaatcct
     4381 gcaacagctg gaagcgcaag gggtcagcat tctgcgtgtt taaagatgct ggcggaaaaa
     4441 tgtcacggca gcatctaacg cctcaggcgt aatgcggtga cgcacgcctg gctgccatga
     4501 acaggttagc agtttatccc gccccgtctc gcttaaggcc tgctgcaaac gtagtgattc
     4561 gtcggcaggc acgacgtcat cgtcaaggcc atgccacagc agtagaggtc tgtcaccaag
     4621 ttgttccagg tggtttgtcg cttcccactc tgccagcggc gcgacaatgt tattgaattc
     4681 attctgctgt gctgccgttt caggtatcag cggtggaaac agtgaacggg cgagcgatgt
     4741 aaaatagccc gatcccatca tgctggcggt acattttatc gtggggtggc gagcggtaat
     4801 tcccagtgcc gtcatcgcgc ccatcgacgc gccaccgact gccagacggt catcacgcag
     4861 ccagtttt
//
LOCUS       DAWDRC010000290         4858 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7569_length_4858_cov_2.340412, whole genome shotgun sequence.
ACCESSION   DAWDRC010000290 DAWDRC010000000
VERSION     DAWDRC010000290.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7569_length_4858_cov_2.340412"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2444..2453
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1174 a         1275 c         1295 g         1104 t
ORIGIN      
        1 cggatgtttc gcttctgcga agtttactcg gtgccgatga acaggctgcc gccgcgctgc
       61 tgttggcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc atgggggatg
      121 aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggac gctgtattgc
      181 tggagacggc ggtagatcta cgctttgcgc cggaatttgt cgctcgccat attgccgaat
      241 ggcagtaccc gaatccgcag gatctgagaa ttactggctg ccaaagcagc gaagggcaga
      301 atgggcaaac gccatttttg ctggcggaaa tgctggtctg gccctgcttc acccaggtgg
      361 agcgctttac tctcgacgca ccgtttatca ccctcgaaca gcgtactgaa catggggtgc
      421 gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgtggt gaagtggcct
      481 tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcggagatt acccgcgaaa
      541 ctgttgccgc ggaagcgcca gcgatacagt gcctgcaact ggcaaggcca gagcaacgac
      601 gcagagcggc gctgattgat gggcaaacgg ttgcagaaaa agcgcaaaag ctatggcagg
      661 actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa atcaggaaca
      721 tgccgcaatg gctggctggc ggcgcaggat ttcagcggca gcacactaac tcattggcaa
      781 atagaaccac agccgatggt tgcggaacag gtgctggatg cgctggtgga acagtggcaa
      841 cgaacgcctg cggaggttgt cctttttcct ccgggctcgt ttggcgatga actgtcgaac
      901 cggctggcct ggcgtttaca tggtgcaagc atctgccagg tgacttcgtt agatattcca
      961 accgtaagcg tgcgcaaatc ccactgggga aatgcgttga cggcgacgct gcaaacagaa
     1021 aaacgcccgc tatgcctttc gctggcacgt caggcgggag cagataaaaa cgccacgttg
     1081 ccctctggga tgcagcaact gaacattgtc cccggcgcgc ttccagactg gcttatcagc
     1141 gtcgaaaacc tgaaaaatgt tacccgtgac ccattagctg aggccagacg ggtgctggtg
     1201 gtggggcagg gcggagaggc agataaccag gaaatcgcta tgctagcaga aaagatggga
     1261 gccgaagtgg gctacagtcg ggcacgagtg atgaacggtg gcgttgatgc cgaaaaagtg
     1321 attggtattt ccggacatct gctggcacct gaggtgtgta ttgttgttgg cgcttccggc
     1381 gcagcagcgt taatggcggg cgtacgcaat agcaagtttg tggtggcgat taatcatgac
     1441 gccagtgcag cggtattttc acaggcagat gttggcgtgg ttgatgactg gaaagtggtg
     1501 ctggaggcac tggtgacaaa cattcacgca gattgccaat aacgtgctgc cggatgatcc
     1561 tgccgatggt tctgggctgg ggacttgtag gcctgataag acgcgtcaag cgtcgcatca
     1621 ggcgctgaat gtcggatgtg gcgtgcagcc tacaaatcac accaaattca acttgttacg
     1681 attatcccgt caaagcaatt ttttcgctaa cgtccagacc ccagtattcg tggtggaaag
     1741 cgccacaaca cccgcgttaa tcgcattacg cgcatcttct tcatcgcaca ccagcccgcc
     1801 agcaatcagc ggttggcgga ttttctccgt cacccagccc agcactttag gcatgcagcc
     1861 tggcaggatc tcaatacaat ccggattcga ttgcgcaact tgcttatcaa tgttgtgaaa
     1921 cgaaatcgaa tcaacaataa acagtcgatg aatacagaaa aaaccttccg ctcttgctgc
     1981 tttcagcatt gaggctttgg tactgataat tccgtccgcc tcggtcacca gtttcaaaaa
     2041 ctgaatcacc acttctttat ttgacgcgcc ttccagcaaa tcaacatgaa taaaagcata
     2101 tttcccggcg ttcttaatct ttttaacgat attactaatg gtgcagatat tgccgtacaa
     2161 cacggaaata aactggcatt cagagtcgat tgccagttgc aggctggcat tatctttaac
     2221 ggcagcaatc accggattct ggcggagcag gtgtaagagg gacataaatt atccttgtta
     2281 tttatccaaa gcggtaggtg atgccgaatc cggaaggcgg atagcgccac tgttgtagtg
     2341 ttgattcgtc gcacagcaaa cggcaggtgc cacactccag gcagccgtga taatcaacgc
     2401 gtaagtcacc ttcaggtgtg agcgaaaaaa gaccagccgg acannnnnnn nnnggcgtta
     2461 atcaaccgtt ctgccgtctg gcgctcaacg gagtcagctg gaacaatgtg cggcgcatcg
     2521 gcagcgcgcc agagattacg ggctacagac atcgcagact cctgataaca tcgcccgtca
     2581 gatgccacaa gccgtgacga cgtaaatgac gccagagcaa ctggcgcagc ggtggaacag
     2641 gtgtattccc ctgatcccat aaatcacggg aaatatcctg cattaaccca ggccacaccc
     2701 gataccagcc aggacgttgc aaaagcgccg gaacatgctg ataacgctgt agaacatccc
     2761 acagcaggct gcgctctacg ttgtgatgat aaagcggaaa cagattttgc ggctcgcggt
     2821 gttggcaggc gcttatcagc gtttgtgccg ccgcctgcgc gccagttagc gccatatcca
     2881 tgccgcgcac ggaaattccg gtattgacgc aactgcgcaa cgcatcgccc accagcagcc
     2941 agccgttacc ggcgtattgc accggcatac tgcgcaggcc accttctggc accagatgcg
     3001 caccatactc cagtgattcc gtgtttttga taagcgggcg cactgccgga tgcgttttaa
     3061 agcgagccag cagctcgctt gccggaacac gactttgcgt aagggaggag agcgggcaaa
     3121 caatccctaa cgagagcgtt tgttgattag tataaagaaa tgcgccgccg ggtaagtcat
     3181 cacagatccc gccgctgaac aacaacgctg cgccttcgtt attctccaga tgaaaacgtt
     3241 cttcaatagc ggatggttcc agcgtcagca cttctttgat tcccaatgcc atcgcttcgc
     3301 cagcaggacg agtcactaac ccgtggcgtt ccgccaggac gctgttggca ccttctgcca
     3361 gcaccacata acgggcgcga agaatatcgt caccacaaat gacaccacac accctgccgt
     3421 tttcttcata cagtgcatct accgtcgcac cggggatgca ttcgacacct tctttttcgg
     3481 cttcggcaac cagccacgga tcgaatcgtg cacgtaatac actccaggat tcaccgccgg
     3541 gctgtaagct ggaaaacgtc gttgcgccat ctggcgttaa cagagaaaga ctttcgtgag
     3601 tgatgcgtcg ttcaagaggc gcggtcaggt gaaattgcgg gaggagttcc gcgagcgcat
     3661 gggtatataa acgcccgccg gaaagatttt tgctgccggg aatctccgct cgttccagta
     3721 acagaacgga taaacccgct cgcgcgcagc gtaacgcgca agcggtgcca gcaataccgg
     3781 caccaataat aataatgtcg cagtcgtctt ccatgtcatg ccccagatat ccagaacaca
     3841 acaccctaac atagcgttac ttaagggaaa ttgaccgccg acacactctt atcattcgcc
     3901 gcgatagata caacctgcgg tgcaggtttc ttttaccatc accgcactta acagcggcac
     3961 aacgggttta acctgatccc aaatccattt tgctaaaact tcgctggttg ggttttccag
     4021 accaggaata tcattgagat aatggtgatc gaggcgctca taagttggtt taaacgccgc
     4081 tttcagttca gcgaaatcga taatccagcc cgtatgcgga tcgacttccc cggtaatttc
     4141 cagtcgcacc ataaaggaat gcccgtgcag gcgaccacat ttatgccctt gtgggacgtg
     4201 cggcaagcgg tgagcggctt cgaaggtgaa atctttaaat aacgtggtgg acatcataat
     4261 ttctctacat gacgataaaa ccgccgtaga gtaccggaaa ggctgttttt tcgcattatc
     4321 taaaacgact ttgttgcgca aaatcgctga tttatcttat tgatgggcaa tattcatttg
     4381 tttttcattg ggttggttaa tctattttgt tgttaaagac tattgctaaa acaggttagt
     4441 cgatttggtt attagttatc gctatcccgt ctttaatcca caccgttcac cccgttaacc
     4501 ttaccttctc ttctgtttta tgggcgctga cagggcgcag aaacagcttt gcttactgga
     4561 acataacgac gcatgacgac acaggtccca ccttccgcgt tgcttccgtt gaacccggag
     4621 caactggcac gccttcaggc ggccacgacc gatttaactc ctacccagct tgcctgggtt
     4681 tctggctatt tctggggcgt gctcaaccag cagcctgctg cgcttgcagc gacgccagcg
     4741 cccgccgcag aaatgccggg tataactatt atctccgcct cgcaaactgg caatgcgcgt
     4801 cgggttgctg aagcattacg cgatgattta ttagcggcaa aactgaacgt taagctgg
//
LOCUS       DAWDRC010000291         4851 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7582_length_4851_cov_2.444746, whole genome shotgun sequence.
ACCESSION   DAWDRC010000291 DAWDRC010000000
VERSION     DAWDRC010000291.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7582_length_4851_cov_2.444746"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1532..1541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1328 a         1219 c          986 g         1308 t
ORIGIN      
        1 tactttgcca ttgatgacaa attcaatgcc gtcgtcggtc cgttctgtgc gataccccac
       61 ctcttctgct ggagtcgcag ccaaacgccc atgtaacccc agcgtttctc cgttatcctc
      121 acagggcgtg ccaatgtagc taagtccgca ggtagtcagt aaccccgcgt agaagctacg
      181 taaaaacccg ttgccctgag actcaaaaaa agcgggggcg acaatgccgt tgggggtgat
      241 aaaagagagc gcagtgtcct tgtagctggc ccatgcaata tccagcgcgc gatcttttac
      301 caccgtaaat gccagtcccg caccagtacg gacatcaaac gcttctgtcc cctttgctct
      361 gccactacgt aactcatagt gtttaattcc ggcaacctgc gagagatcgc caatccacga
      421 ccaaatttcg tttttcattg tgctgcttcc cgaatggaca ggtcatggcg ctggatgaac
      481 gtttcgattt ccataaggcg agacgttccc tccattgggc cacgtcgggt taccgccagt
      541 gcaccgcagg cgttggcata ttgtaatgcc cgatgggcgt caaaccctag ctggcggcag
      601 gcaatccacg cgccaccaaa gcagtctccc gcaccggtcg ggtcaacttc ttccaccgga
      661 tagctctcaa catgaaactg ctcattcgcg gagtagtaac tggcaccttg attgccgcgc
      721 ttcacaatca cctctttaac accttcttca agaaaaccag ctattgcacg ctccggcgtc
      781 gagtgtggcg acaagagcaa cacttctccc tcgctgggca tatagatatc tgttaactca
      841 agaacaaagt ggagggcgtc acgcatttct ggaatatcaa gcatttcttt acgaatattc
      901 gggtcgaaag aaataacgcc accatttgct ttaacgatgg ttactgcttt ttttactgcg
      961 tctaccatat ggaatgaaaa cagggatgaa cccataatat gaaaatgggt acaatccttc
     1021 agaatatttt catcaacatg ctgtgccgat aatttaccgc aagcggcatt cttgatatta
     1081 aaaataaaat cgcgatcgcc agagttatga taagtaacaa aggcactacc ggtggcttcc
     1141 agaggtaaga ctgaaatccc cctgatgtcc acaccgtcag cagccagacg atgtatatta
     1201 atatcaccaa agccatcatt ccccacgcag ctaatgatgc cgcacgggac gccaagacgt
     1261 gtcacctggt cgataaaaat agcgggcgca ccactgggat aagggccgtt ccagatacct
     1321 ggttgatcaa atggctggcc gattttactc gccataattt caaccagaat ttctccaatt
     1381 gtgaagacct tactcatatg attaattcct tgaaatgatt agctgcgttt tcttttattt
     1441 cttacgtcaa aataaacggc tacaagaatg actaatcctt taaccaccat ctggtaaaaa
     1501 taaggaacat tcattaagtt catgccatta tnnnnnnnnn nttattaata acgccgatgg
     1561 ttaatgcgcc gatcaccgtc cccaccacgg ttccatagcc gccgttcagg ctggtgcctc
     1621 ccagtacagc tgcggcaata gcatccagtt cgtagctcaa cgcggaattc ggttgcccgg
     1681 aatagaggcg tgaggcaagg atcatcccgc tgatacctgc cagcacggct gtaatcataa
     1741 agactttaat tttcaagcgg gtgacattta tccctgaata aattgctgct tctttattac
     1801 caccagtgat ataaattttt cgtccaaagg ttgttttagt aagaatgaag tggtttatca
     1861 gcaataagat gattaatatc catataggaa taggtatata gagaaattct ccattcccca
     1921 gcgccagaaa ggcatcatct ttaatcatga tgggcatacc atcggtcact atataagcga
     1981 taccacggaa gatccccata gtagcaacgg tgacaataaa cgacgggatc cccgctatgg
     2041 ccgtcaagcc accatttatc atccccagag ccagtgctgc aataatggat aatgggacca
     2101 ataaaataaa tggaattccc catgaagccc caagcgcagc aaatgtgcca atcaatgcaa
     2161 tggttgaacc aaccgataaa tcaatatcgc caagcaaaag tacgtaagtc attccatagg
     2221 cggtaattgc aattatcgaa acctgaagaa taatattagt aatgttattg gcggtaaaaa
     2281 aactgtcatt aataaaactg aacagaatga ttaacaccac cattccactg aggataccac
     2341 catattgcct gaagactttt ttaaaattat ttgcaatggg aatttccgaa ggtttcggac
     2401 tggcaagagt tttctctgtc atttaacgta ctcctgttgc agcggtcatt acctgtgtct
     2461 gcgttaaatt ttcacatgaa taaatatcgg caatcgtttt attacgcatt accaccagac
     2521 gatcgctaag tgccatgact tctggcagtt cagaggacac cagaataatt gctgtccctg
     2581 ccgcagccag ttgacgtatc acgttataga tttcaaattt tgcgccgaca tccactccgc
     2641 gcgttggctc atcgagcaat aaaatacgcg gagttttctc cagccacttc gataacacga
     2701 ttttctgttg attaccgccg ctcagtgctc ccactgcctg atgcacagaa ctcactttag
     2761 tgttcattct ggcaattgat tgcagcgtct tcgcctcttc ttgtcggtga ttgatgagtc
     2821 ccatccgcga agcgttatat tgaaacgcca catggtgcgt gttcatattg acgtcgtgca
     2881 tcagcactaa cccttcttcc ttgcggtttt cggtcacaaa agcaatgcca tgatcaatag
     2941 cctgtttggg attagcgatc ctgacttcct taccatcaat caacacagtc ccgtgatacg
     3001 aaacgaggcc aaatagcgcc ttcatcacat cggaacgccc ggcccccacc aggccaaaaa
     3061 atccgaggac ttcccccgga cgcacagcga aactgacctc tttaaaaagc gaaggatggg
     3121 aaagattttt tacttccagt tttgcaggta cattttcatc cggtttttcc gcaagacgtg
     3181 ctggccaaat atttttcatt tcccgaccaa ccatttgggc aatcaagtgc tggatatcac
     3241 tgtttttctt ttcatgcgta gcgatatact caccgtcgcg gatcaccgaa atatcatctg
     3301 aaatccgcat aatttcatcc atgcgatgag agatataaat aaccgcacag cctcgctgct
     3361 ttaatttttc aataatgtta aacaggatct ccgcttcatt atcacttagc gaagatgtcg
     3421 gttcatccat aatcacgaca tccgctgcat agcttaaggc tttagcaatt tctaccagtt
     3481 gcctatgtgc catcgacata ttgccgacca tttccagagg gtctagtggg atgcccagtt
     3541 ccgcgaggag gttctggcaa tctgcgagca tttttttatc gttaatgatg ccaaaacgtc
     3601 tcggttcatt attggcataa atattttccg ccacggtcat attattactc aggctcagtt
     3661 cctgaaaaat aatcgcaatc cctaactggc gagaatgctg cggattatta atggtgatgg
     3721 tttcaccacg tagtgttatc gttccctggg ttggcggata aacaccggcc agtattttca
     3781 ttaatgtcga tttaccggca ccattctcgc ctaataaagt atgcacccgc cctttacgta
     3841 aagagagtgt aatatctttt aatgcctgaa catgaccaaa tgtcttcgca atatgcgaaa
     3901 catcaagaat aacatccgtc ataatgacct cggtgtcaca acaaaacgcc aataacgttt
     3961 tgttgtgagg tgaagatatt tactatttac ctgtataaac gccgggttgt accgggatca
     4021 ttttttctac cggtttacca ttgaggtgat caacagcggc atcaatggct ttagccccca
     4081 tttgatcggg atattgccga atcacggccg caaacgtttt ttcactgtca acagcatcac
     4141 gcgcttcttt catgccgtca aaaccaatga ttttgatgtt gtcgttatgc gcagattttg
     4201 ctgcgataac cgctgccagc gccgcatcat caccaaagcc aaacagacca ttcaggttgg
     4261 catttgcctg cataatgttt tgcgcagtcg ttaacgcttc agcccgcgta ataccaggct
     4321 gttcagcaac aattttaata tccggtgtat cgcttaaccc tttcttaaaa ccatcaacgc
     4381 gcgcaaccac cgattgtagt gccggataag aaataatggc tacagtacct ttaccatgca
     4441 aaagtttacc cattaacttg cctgcttcaa cgcctcccgc atagttatcg gtcgcaacat
     4501 gcgatacgac cggaacgcct tccgcagcga cgtcaacggt gataacggga ataccggcct
     4561 tatcggcttt aattatcgct gcctggacgc ctttcgaatc gaccggagag ataataatgg
     4621 catccacttt tttagtaata aaatcctcaa catcagaaag ttgtttgttt aaatcctgat
     4681 tggcaataga gacacttaaa ttgacattgt ctttttttgc ctcttctttc atggcattcg
     4741 caagatcaat atagaaagga tgttgttgtg ttaataatga cgcaccaatt ttatactctt
     4801 ttgccaacga actttgcgag aacaatgcca ggctcaccaa catagctgaa c
//
LOCUS       DAWDRC010000292         4850 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7587_length_4850_cov_2.106569, whole genome shotgun sequence.
ACCESSION   DAWDRC010000292 DAWDRC010000000
VERSION     DAWDRC010000292.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7587_length_4850_cov_2.106569"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1281 a         1195 c         1288 g         1086 t
ORIGIN      
        1 ttatttttga ctgtatcatt gtcacaggga aagtgcatgc tgcgatggaa acgctgtatt
       61 atgctaacat ttatctctgg tgctgctttc gcggcaccag agataaatgt taagcaaaac
      121 gaatcgttac ctgatttagg tagccaggca gcacagcagg atgaacaaac caacaagggt
      181 aaatcgctga aagagcgcgg agccgattac gtcatcaact ccgccacgca agggtttgaa
      241 aacttgaccc ctgaggcgct ggaatctcag gccagaagct atctgcaaag tcaaatcacc
      301 tcaaccgcac aatcttatat tgaagacaca ctctctccct acggtaaggt ccgttcgaac
      361 ctctccattg gtcagggcgg cgatctggat ggcagttcca tcgattattt tgttccctgg
      421 tacgataatc aaaccactgt ttatttcagc caattttctg cgcaacgaaa agaagatcgt
      481 acgatcggga atattggcct tggggtaagg tataattttg ataaatatct attgggtgga
      541 aatatatttt atgattatga ctttacccgt ggacatcgcc gtttaggttt aggcgccgaa
      601 gcctggacgg attatttaaa attctcaggc aactattatc acccactttc tgactggaaa
      661 gactctgaag atttcgactt ttatgaagaa cgccctgcgc gcggttggga tattcgcgct
      721 gaagcttggc tacccgctta cccgcagctt ggcggaaaaa ttgtcttcga acaatattat
      781 gggaatgaag tcgcgctttt tggtaccgat agtctggaga aagatccctt cgctgtcacg
      841 ctgggtgtga aataccagcc cgttccgctc attgttgtcg gtacagattt caaagctggc
      901 actggcgata ataccgatct ttctgttaac gcaacgctga actaccagtt tggcgtcccg
      961 ctcaaagacc agcttgatcc tgacaaagtg agcgcggctc actcgctcat gggtagccgt
     1021 catgactttg ttgaacgtaa caactttatt gttctggaat acaaagaaaa agatcccctc
     1081 gacgtcattt tgtggctgaa agcagacacc acgaatgaac atcccgagtg cgtgattaaa
     1141 gatacgcctg aagaagctat cgggctggaa aaatgtaaat ggaccattaa cgcgctcatt
     1201 aaccatcact acaaaatcgt tgctgcctcc tggcaggcga aaaacaatgc tgcccgcacg
     1261 ctggtgatgc cggttatcaa agagaatacc ctgacagagg gtaacaataa ccactggaac
     1321 ctggtgctgc ccgcctggca gtacagctct gacaaagccg aacaagaaaa actcaatacc
     1381 tggcgagtac gtctggcgct ggaagatgaa aagggcaatc ggcagaactc tggcgtggtg
     1441 gaaatcaccg ttcagcagga ccgtaaaatt gagctgattg ttaataacat cgcgaacgtg
     1501 ccggaagaga acaaccacag ccacgaagcc agcgctcagg cagatggcgt tgatggtgtg
     1561 gtgatggatc tcgatgtaac cgacagcttt ggcgataaca ccgaccgcaa tggcgatgcg
     1621 ctgccggaag ataacctcac gccgcagctt tacgacgcgc aggacaaacg agtgacgtta
     1681 accaacaagc cttgctcgac cgacaatccc tgcgttttta tcgctaaaca agataaagaa
     1741 aagggcactg tcaccctctc cagtacctta cccggcacct atcgctggaa agcaaaagcc
     1801 gcgccctacg atgacagtaa ctatgtggat gtcactttcc tcggggcaga aattggtggg
     1861 ctaaatgctt ttatctatcg agtgggggcg gctaacccca gcaacctgat aggtaaagat
     1921 aaagaaccgt tgccgcttaa caacatttat cgatttgttt tatggcgcga caacaataaa
     1981 gacggtgtct tccagcaggt cgaaaaactg actgacgaag agatggcgca gtacgactac
     2041 aagtgggaat ttaccggcaa gagcatcaat ggtgaagtgg gtgcacaggc gaacactttc
     2101 aatgaggaca ttgtgattcc ggccactaac cgtgaagcgg cgcaaaccta tggcgcacaa
     2161 gcgggagatg gcttgcaggg gtacggttta cgcgtgctgt ataccaaaaa ataagattgg
     2221 cgtaaggcca gtcggccagt tgctgaaggt tcattaagga gagcatcaat gaagcaggat
     2281 aaaagacgcg gtctgacccg gatcgcatta gcgctggcac tggcaggtta ttgtgtggca
     2341 cctgtggcgc tggctgaaga cagtgcctgg gtcgacagcg gcgaaaccaa tattttccag
     2401 gggaccattc cgtggctcta ttcggaaggg ggaagtgcta cgacagatgc cgaccgtgta
     2461 acgttgactt ctgatctaaa aggcgctcgc ccgcaaggca gtgaaacgga caagcgtctt
     2521 tactcgggtg ataaattaac cgtaagttgg gaaattggtg ataccgaagg cgacgtggat
     2581 cttgggggat tgggcgataa cgcgaaaact atcgatacta tccgctggat gagctacaag
     2641 gatgcgcagg ggggtgatcc aaaagagctg gtaacgaagg tgaccagtta cactcttacc
     2701 gatgccgacc gtggtcgtta tatcggtatt gaaattacgc caaccacgca gaccggtacg
     2761 ccaaacgtcg ggactgcgct gcatctttat gacgtttcta ctgccagcgg cggcggaagc
     2821 gacagcgata acgttgcacc ggggccggtg gttaaccaga acctgaaagt cgccatcttt
     2881 gttgatggta ccagtatcaa ccttatcaac ggtagcacgc caatcgaact tggcaaaacc
     2941 tacgtggcca aactgtactc ggatgagaac aaaaatggca agtttgatgc gggtaccgat
     3001 gctgacgtca ccgccaatta tgacttccgt tgggtacttt ctggcagcag ccaacagctt
     3061 ggcacttcgg gtggcatcgt taactcaagc ttcgataata acaatttggt catccctgcg
     3121 accaacgacg aagccagaac caaccttaac ggccctgcgc gcgatggaaa agaggcactt
     3181 tccatcccga ccaacggcga cggggtacag ggttacaaac ttcacattat ttacaaacac
     3241 aaataacgcg tactcagcag cattcccgca agggaatgct gtttccgcat attttgctta
     3301 atcccggatt aagcaaaata tgttgatgcg acaagcgtcc gtttaacaac cggtggagtt
     3361 tctgtcatgt ccctgaccct gaataaaatc cttgtgttat gcgcattgct gataagcgca
     3421 atgctgcctg ggtggtcatg ggcagaatct gcatggcaag actcttcaga tacggtcggc
     3481 gaatttaacg gtaccgtgcc caccgccgac agcgccagca tacccgtcta ccagggcagt
     3541 gtctttctgg atcctacaaa gacgcatgag gtagcgttta ccgctaaacc gagtgaattc
     3601 aacgcggatg ttagcgtctc taaattgctc gtcaccaacc cgcaagacag agaaggcgat
     3661 atcattgcca ccccacgttg ggaaaaccaa acgccaccgg ctgtatcttt agtttgggcg
     3721 gatgccgcca cgccggacac cttactggac ccacagccag tcgcagatcg ctcgttttgt
     3781 gcacagggac ttgccgggcg ttcgctggtc gcctgggcgc agcctgatcc ccagcagacc
     3841 atgccgctgt tatatttact cacctcgacg ggctatccct atgaaagtgt tctgacgctc
     3901 gccgatcaga aagtgacgct gaagattgcg cccgcgcagg gtgaccttat ttccgtcagc
     3961 gctgcgggct atgacgaatc ctcaggtgcg gcgaaaatga cggttggggg cagtatcacg
     4021 ttaacggtca ccaccaaaga ttgcgtcggt aatgttgtgg ggaatattcc ttttgttatc
     4081 aaacgcaaag atgctgaaaa ccgccagggt gtggtgaaca atactgcgcc tgtaaaactg
     4141 ggcacgaccg aattaaccac caccgcgacg gaatatcgtg gtactaccga tgcgaatggt
     4201 gttgccacgg ttacggtcac ccaggccaac ggccctggtg taaaaacgcc gttggtcgcg
     4261 agcctcgcgg gtattgctca ggccagtgaa acggcagtga tctttaccgt cctcaccagc
     4321 ccggatgttc cgcaggctac aatgtggggt catatgcccg atacgctgaa ggcccgtgat
     4381 tacactttta gtcgaccaaa actggcggcg gaagtcgata atgaaggcgg tacggttaac
     4441 gatcacaatg aaacctggtc cacctttacc tggagcggcg cggataaaca ttgcgacatt
     4501 ttgcccggaa tgcgtcagtt cggtgcgctg gcgacggtgg tgccaacgtc agtgcaggat
     4561 gtggctggtt ggccgatgca gggtaatttc tactggtcat cactggcggg gatgagcggc
     4621 cagcaccatg cggcggatgt ttcgaaccgc agcgaagcgc aaaaaccgga cgatacgacg
     4681 tttattgtga gctgtgtcga taaagaagcg cccgatgttg aaccgaagct ggtactgacg
     4741 ccgggcagct atgattcgac gataaaagcg atgaaagtaa aggtggggga agaggcttct
     4801 ttacgtctga cgattaccga tagcaagaac aacgatcagc cgctggctta
//
LOCUS       DAWDRC010000293         4791 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7668_length_4791_cov_2.392314, whole genome shotgun sequence.
ACCESSION   DAWDRC010000293 DAWDRC010000000
VERSION     DAWDRC010000293.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7668_length_4791_cov_2.392314"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1676..1685
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1230 a         1222 c         1218 g         1111 t
ORIGIN      
        1 gattgcttga cttaacaaac agataacttg acagaaagga taaaaaaaga gccagcgggc
       61 gctggctctt gaagacacta aattagaagc tggcgttacg cggagtacgt gggaacggaa
      121 taacatcacg tacgttttgc acgccagtta cgtaagcaat caggcgttca aaaccaagac
      181 cgaaacctga atgcggaaca gtaccgtagc gacgcagatc gcgataccac cagtaatctt
      241 ctttgttcag gcccatttca agcatacgct cgtccagcac gtccagacgt tcttcacgct
      301 gggagccacc aatgatctca ccgatgcccg gagccagaac gtccatagcc gcaacggttt
      361 taccgtcttc gttaaggcgc atatagaacg ctttaatatc tttcggatag tttttaacca
      421 ctaccggtgc tttaaagtgt tcttccgcca gataacgctc atgctcagaa gagagatcga
      481 cgccccagta aaccgggttt tcaaacttcc tgccgcagtt ttcgagaatg gtcactgcgt
      541 cggtgtaatc cacctgcgcg aaatcggcct caatgaagcg ttccagacgt gaaacggcat
      601 ctttatctac gcgttcagcg aagaatttca tgtcgtcagc gcgttcttcg agaaccgctt
      661 tgaagacata tttcagcatg gcttcagcca gacctgcgat atcgttcagg ttagcaaacg
      721 ccacttccgg ctccagcatc cagaattccg ccaggtgacg gctggtgttg gagttttcag
      781 cacggaaagt cgggccgaag gtataaattt tggacaatgc gcaagcgtag gtttcgccgt
      841 tcaactggcc agatacggtc aggaaagact ctttaccaaa gaagtctttg tcgaaatcca
      901 ctttgccctg atcgttacgc ggcaggtttt ccagatccag cgtagaaaca cggaacattt
      961 cgcctgcacc ttcggtatca gatgcggtaa tcagtggcgt tgaaacccag aagaatccct
     1021 gctcgttaaa gaagcgatgc agcgcctgcg ccagcgtatg gcgaacgcgc gcgacggcac
     1081 caatcaggtt tgtgcgcgga cgcaggtgag cgacttcacg cagatactca atgctgtggc
     1141 gttttgccgc catcgggtaa gtgtctggat cttcaaccca accagcaact tcaaccttgc
     1201 tggcctgaat ttcaaattgt tgcccctggc ccggcgacgc cacgacttta cccgtcacaa
     1261 tgaccgagca gccggtggtc agacgcagga cgtcttcatt gtaattgggc agagaattat
     1321 tgatgacagc ctgtacagga tcaaagcagg aaccgtcata aacggcgagg aaggagatgc
     1381 cagcttttga atctcggcgg gtacgtaccc atccgcgcac ggtgacttcg ctgtcaacgg
     1441 ctacgcggcc ctggagtacg tcggctacag gcacaacgct cataatattc tctctgttaa
     1501 tagtcggaaa aaataaacac ttgtccaccc gtaaatgggg ggatacctat gttacctggc
     1561 atctgcaatc agacaagcag aattcgcaaa tgcagcgaaa gatttcggaa ttaaaggtaa
     1621 aaaaagggag ccgattagct ccctgatgat attaactggc ttttttaata tgcggnnnnn
     1681 nnnnnaacgc tttgcgcagc gcccgaacaa acgctttatc gtggcagata gttttgccag
     1741 ggctgtcaga aagtttcgcc actggtttac cgttacactc taccaactta atgacaatat
     1801 tcaggggttt tacctggggg atatcgcagg tcaggcgagt cccaatacca aaacttaatt
     1861 gcacgcggga agagaagtgg cggtatagct caaccgcttt gcgtaaatcc agattgtcag
     1921 agaaaaccag cgttttactc tgtggatcaa ttcccagctt ttcataatgt gcaatggctt
     1981 tttcacccca ttcaagcggg tcgccagagt catggcgcag gccctgatag cgactggcga
     2041 actcgacacc gaaatctcgc aggaaagcat ccatagtgat gcagtcggtt aacgcaatgc
     2101 caagttggtc gggatactct tccagccagg cagcaagtgc agctcgctgg ctgttggcta
     2161 gatccgggct gatttgctga tgcgcctgga accattcgtg tgcctgtgtt cccatcggcg
     2221 tgagggaaag ccgacgcgcc agatcgtagt tgctggtgcc cacgaaccag gattcctgtt
     2281 gcagacgttt cacgatggtt tcttgtactt cgcgagaaaa acggcggcgg gtgccaaaat
     2341 ccatcagatg gaagcgcgac atatcaagac cggcggttaa tgccgagaag tcgactaatt
     2401 tgttttccag agtgtcgagg gcttgcgcaa cgtcggcctg cggtgagcga tagcgatgta
     2461 ccatttcact gataaccgcc agcaaaggaa cctcccagag gatgacttca cgccacgggc
     2521 cgcttaaacg aatatccagc ttgccattat cgttggacac ggtgacttgt tccgggttaa
     2581 agcggaactc gcgtagccag ttaagatagt cggccttaaa gaaaggcagg gcagagagcc
     2641 actgatattc atcatcctgc aggcgcaggt gctgcatcgc ctgaacctgt tcacgaatag
     2701 catcggcata aatacccagc agatcgtcac ctcggcaacg aaactccgcc gcgacatgca
     2761 catcgtaata gtgatgaaac acggcttgct gcatatgcaa cttataagca tctgtatcca
     2821 gcaacgagtg cagaacagga gaagcgaatt gtgtcatagg tgcgcagtag cgtcctcgta
     2881 caggagcgtt tagtacaata aacatcttca ggaactgctg gagtatacct tgtttcgcaa
     2941 tttattgaac cccgatcaca ccatatgcca cctttctggt cgatggcata acgcggtgta
     3001 tgttataaaa atgtagcaat aaaggcgttt gtacctgaaa agatgaagat tctgcatagc
     3061 gcgatttacg caacaggaat agactgaaca ccagactcta taaaagatgc taaaggttat
     3121 ttatgactca acagccacaa gccaaatacc gtcacgatta tcgttcgccg gattaccaga
     3181 ttactgatat tgacttgacc tttgacctgg acgcgcaaaa gacggtcgtt accgcggtca
     3241 gccaggctgt ccgtcatggt gcatcagatg ttccgcttcg tctcaacggc gaagacctca
     3301 aactggtttc tgtttatatt aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg
     3361 cactggtcat cagtaatttg ccggagcgtt ttacgcttaa gatcgttaat gaaattagcc
     3421 cggcggcgaa tactgcgctg gaagggcttt atcagtcagg cgatgcgctt tgcacccagt
     3481 gtgaagccga aggtttccgc catattacgt attatctcga ccgcccggat gtgctggcgc
     3541 gttttaccac caaaattatt gccgataaaa ccaaatatcc cttcctgctt tccaacggta
     3601 accgcgttgc gcaaggtgaa ctggaaaatg gacgtcattg ggtacggtgg caggacccgt
     3661 tcccgaaacc gtgctacctg tttgcgctgg tggcaggcga ctttgatgtg ctgcgcgaca
     3721 ctttcaccac gcgttctggt cgcgaagtag cgctggagct gtatgtcgat cgtggcaacc
     3781 ttgatcgtgc gccgtgggca atgacctcgc tgaaaaactc catgaaatgg gatgaagaac
     3841 gcttcggcct ggagtatgac ctcgacatct atatgatcgt cgcggtggat ttcttcaata
     3901 tgggcgcaat ggagaataag gggctgaata tctttaactc caaatatgtg ctggcccgca
     3961 ccgacaccgc caccgacaaa gattacctcg atattgaacg cgttatcggc catgaatatt
     4021 tccataactg gaccggcaac cgtgtcacct gtcgcgactg gttccagctc agtctgaaag
     4081 aaggtttaac cgtcttccgc gatcaggaat tcagctctga ccttggttcc cgcgcggtaa
     4141 accgtatcaa taatgtgcgc accatgcgcg gattgcagtt tgcagaagac gccagcccga
     4201 tggcgcaccc aattcgcccg gatatggtca ttgaaatgaa caacttctac accctgaccg
     4261 tttacgagaa gggcgcggaa gtgattcgta tgatccacac tctgcttggc gaagaaaact
     4321 tccagaaagg gatgcagctc tatttcgagc gtcatgatgg tagcgcagcg acctgtgacg
     4381 actttgtgca ggcgatggaa gatgcgtcga atgtcgatct ttcccatttc cgccgttggt
     4441 acagccagtc cggcacgccg gttgttactg tcaaagacga ctacaatccg gaaactgagc
     4501 agtacaccct gaccatcagc cagcgcacgc cagctacacc ggatcaggca gaaaaacaac
     4561 cactgcatat tccgtttgcc atcgaactgt atgacaacga aggcaaagtg atcccgttgc
     4621 agaaaggcgg tcatccggtg aattccgtgc tgaacgtcac ccaggcggaa cagacctttg
     4681 tttttgataa tgtctacttc cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag
     4741 tgaaactgga atataagtgg agcgatcagc aactgacctt cctgatgcgt c
//
LOCUS       DAWDRC010000294         4786 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7676_length_4786_cov_2.536250, whole genome shotgun sequence.
ACCESSION   DAWDRC010000294 DAWDRC010000000
VERSION     DAWDRC010000294.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4786)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4786)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7676_length_4786_cov_2.536250"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    468..477
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3300..3309
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1335 a         1050 c         1126 g         1255 t
ORIGIN      
        1 cagatattgt ccatattggt tttggcgctt ttcatcgcgg acaccaggct gtctacacgg
       61 atctcactaa tcagctttct gacacccact gggggatctt tgagatcaac ctgtttggtg
      121 atgctcaact gatcgaaaac ttaaatgcgc aaaatgggct gttttcggtt gtggaaacat
      181 ctgcatcgca atctacctca cgccttgtgc gttcggtggc tggcggtatt cataccccca
      241 gagatggcat tgccgcagca atccataaac tggctgaacc tcaggtaaaa atcgtttcat
      301 taactatcac cgagaaaggc tattgtctcg atccgcaaac gcgatcactt gatctcacca
      361 atggattaat caaccacgat ctacaaaatc cggatgcgcc tcagtcggct attggcgtga
      421 tcgtctgtgc tttgcaacaa cgtaaagcgg caggactcgc cgcttttnnn nnnnnnngtg
      481 acaacttgcc agacaatggg catctgacgc gcaatgccgt tctcggtttt gcccgacaac
      541 tggaccagcc cctggctcag tggatcgaag aaaatgtctc atttccaggc acgatggtag
      601 atcgcattgt tccggcaatg actgaatcgc aattcgcctt actggaaact aaaaccggtt
      661 atgccgatcc ctgcgggatc gtctgcgaat catttcgtca gtgggtgatt gaagataatt
      721 ttgtgcatgg gcgaccggaa tgggataaag caggcgcgat gtttgtcagc aatgttcagc
      781 cttatgaaga aatgaagtta cgcatgttaa acggtagcca ttcatttctg gcctataacg
      841 gctcactggc tggctatgaa tttatctggc aatgcatgga agacgctgat tttcgttcca
      901 ttacccgcca gctgatgatt aatgaacaag cccgaacact taatccagac ttaaatatcg
      961 acgcccagga atacgccgac ctgttaattg aacgctttag caaccgtaat gttgcacacc
     1021 gtaccgggca aattgctatg gacggttcgc aaaagcttcc ccagcgagcg ctgactccct
     1081 ggctgaaatt gcatcagcaa aaacaaaaca atgctgttct gtcactgctc gtggctggtt
     1141 ggttgcatta tgtcattgaa gctgttgaga aaagccagtc tgtcgctgat ccaatgaatg
     1201 accaatttca ggcgctaata aaggaacaac aagacgcatg gcaacaggcg ctcgcattac
     1261 tgcaccttag cgccatattt ggtgatttaa gcaaccatca gccatttata aatgaaataa
     1321 aaatcgcctt tgcgaatata aaaaacaaag gcatcaaggc caccatcagc caattattat
     1381 cggatgagca gaaatgaaaa cattaatttg tcagcagcct ggcgttatgg aatatgtgga
     1441 aaaggatatt cccacaccag cagataatga agtgctgtta aaaatcaaag ctgtgggtat
     1501 ttgtggtact gatattcacg cttttgccgg cagacagcct ttttttagct acccacgtgt
     1561 attaggtcat gaaatatgcg ccgaagcggt ttcgcgaggc agccagtgcc aaacagcaca
     1621 gctaggccag cgctattccg tcatcccatg cattccgtgt ggcgagtgcg cagcctgtcg
     1681 ggaagagaaa acgaactgct gcgaacgtgt ttcgctgtat ggcgtgcatc aggatggggg
     1741 ttttagtgag taccttgcgg tacgtgaaga caaccttgtg cctctccctg acgaggtcag
     1801 cgacagtgac ggagcattgg ttgaatgttt cgccattggt gcacatgccg ttcgtcgggc
     1861 agagatcaag gctgaacaaa acgtactggt gattggcgct gggccaatcg gtctggctac
     1921 cgcagccatc gccagggcta aaggggcaca tgttgttgtt gctgatattg actgtcaacg
     1981 tcgccagcac gttgtggatc atctggcgat taatgccttc aatcccacac aggaagattt
     2041 tattgccgcg cttcgtgagg cctttggagg cgaactggct tgcgtagtac tggatgcgac
     2101 gggaaataaa gcttcaatga gtcatgatgt aaatcttatt cgtcatggcg gcaaaattgt
     2161 tttcatcggt ttgtacattg gtgaacttgt tattgacgat ccgaccttcc ataaaaaaga
     2221 gacaacgtta ctcagcagcc gcaatgccac acgggaagat tttgcgttgg tgattaaact
     2281 gatgcgcagc aataaaattc acgaaaattt aatgaaaaac caggcgttcg atttctttag
     2341 tgttggcgaa gattaccagc gtaacgttgt agaaaataaa aatatggtca aaggtgtgat
     2401 cactttttaa gggaacaaat atgacaactg tttatgtcag tgaagaaaat ttaaaatcac
     2461 tggtgcacca caaattacat accgccgggc tggataccga caccacacaa caggtgacag
     2521 atgttttagt acatgccgat attaccggtg tgcattcaca tggtgttatg cgtgtcgaac
     2581 attattgcac ccgccttgct gccggagggt taaataaagc cccgcagttt agtattgagc
     2641 aaatttcacc gtcagtggcc attctcgact ctgacgacgg aatggggcat tccgcattaa
     2701 ttagcgccac tgagcacgct attaaactgg cccagcaaga gggtctcggt tttgttagcg
     2761 ttaaaaacac gtctcactgt ggtgcgttat cttattttgc agagatgatc accaacaaag
     2821 ggttggttgc tatcgtaatg acgcaaaccg atacctgcgt ggctccccat ggcggcgccg
     2881 agcgcttttt gggaactaac cccatcgcct ttggtttccc ggtggaaaac agccatccga
     2941 tgattgttga tatggcgacc agtgccacgg ctttcggcaa aatacttcat gcaaaggaaa
     3001 ccggaaaaca tattggtgaa gggctggcga tagataaaaa cggttatggc accactgatc
     3061 cgtataagat tgaaaacctg ctacctttcg gccaacacaa aggttcaggc attgcactgg
     3121 ctattgatgc actgactggc gtgctgatga atgcgaattt tggcaaccat attgttcgca
     3181 tgtatggtga ttatgacaag atgcgtaagc tggcaagttt ggttattgcc atcgatccga
     3241 aaaagctcgg caatcctgtt tttgcaaaaa ctatggcgaa aatggtcacg gagctgcatn
     3301 nnnnnnnnnc cgttaaaccg gctcctggtg tcgaaaaagt gttagcgccg aacgatccgc
     3361 aaatgcacta caaagaaaaa tgccaacagg aaggtattcc ggttccggca ggaatattcc
     3421 attatctggc agagaattaa tcactatttt ttattataac gatagaaaag cgctgtattt
     3481 atcgtttctg ttatattaac caggagcagg atatgaaagc acttcgtcct ctgacggcat
     3541 cgcttatgct gttaaccagt tgtttattaa tatctaatac cacactggca aaaacaacat
     3601 taaaactgag tcataatcag gataaaagcc atgctgtcca taaagcatta agctatttag
     3661 cggataaaac caaagagtat tctaatggtg aattagttat tcgcatttac ccaaatgcga
     3721 cattaggtaa tgagcgtgaa tcactggaat taatgaactc cggtgcatta cagatggtta
     3781 aggttaatgc cgcatcacta gaatcatttg ctccggatta cagccttttc agtctgccgt
     3841 tcttattccg cgaccgtgat cactattatc gtgttctgca aagtgattta ggtaaaaaaa
     3901 tacttaattc atcagaaagc aaaggttttg ttggtattac gtactatgac ggtggtgcga
     3961 gaagttttta ttccaataaa ccgattacaa aacctgaaga tttagcggga atgaaaatca
     4021 gggttcaaca aagccccagc gccattgcaa tgatgaaagc actcggtggt gtcgctaccc
     4081 cgatggcgca aggcgaactc tacaccgcac ttcagcaagg agtggttgat ggcggagaga
     4141 acaataccgt tgtctatgcc gatatgcgtc acgccgaagt cgcaaaagtc tattcacgtg
     4201 atgaacacac catggtaccc gatgtgttaa ttatcagcac cgacgtgttg aataaacttg
     4261 gtgataaaga gcgcacggca ttattaaaag cagccgatga gtcaatgatg cagatgaagg
     4321 acgtcatttg gcctgcggct gaaaaagaag cctacgacaa aatgaaaggg atgaacgcaa
     4381 cagttgttga tgttgataaa tccgctttca aagaacgcgt caaaccgtta tacgatgaat
     4441 tcaaggcgaa agatgcacaa tcagccaaaa accttgagct agtagaaagt atgtaaccct
     4501 aatgggcagg taattcctgc cttttcatgg aagacttatg cgaacactca ctcatattct
     4561 taataagatc cttgccggat gttgctgcat tattctcgcc attatggtgt tctgcgtaac
     4621 ctggcaagtt attgcacgtt ttatttttaa cgctcccagc actgtgcttg atgagttcac
     4681 ccagatttta tttatgtgga tgattttgct tggcggtgta tatactgcgg gtttaaaaaa
     4741 acatctggcc attgatttgt tggctcaaaa acttccggct gcatct
//
LOCUS       DAWDRC010000295         4781 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7685_length_4781_cov_2.007406, whole genome shotgun sequence.
ACCESSION   DAWDRC010000295 DAWDRC010000000
VERSION     DAWDRC010000295.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7685_length_4781_cov_2.007406"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1616..1625
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1998..2007
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1063 a         1171 c         1319 g         1208 t
ORIGIN      
        1 gtgaccagtt tgttccatat ggaaaaatgc gcccacgatt taacggactg gaaactgtgg
       61 ccgcgtaacg ccattaccca ccgcttctcg ctggaacagg cgggagatgc ttatgcactg
      121 atggcaagcg gcaaatgcgg gaaagttgtg attaatttcc cggattaagg agtctgcgga
      181 tgaccatcta taccctatct cacgggccgc tagagctgga tgtctctgac cagggcggtg
      241 ccatagaagg tttctggcgt gatacgacgc cgctgctacg tcctggtaaa aaaagcggtg
      301 tggcaaccga tgcctcttgt tttccgctgg taccatttgc aaaccgggtg agcggcaacc
      361 gttttgtctg gcaggggcgt gagtatcaac tgcaaccgaa tgttgagtgg gatgcgcatt
      421 atctacacgg cgatggctgg ttgggcgagt ggcaatgtgt ctcgcgcagc gatgatagct
      481 tgtgtctggt gtatgaacat ctcagcggtg tttatcacta tcgggtaagc caggcatttc
      541 atttaacggc ggatacgttg acggtgacgc tcgctgtcac caatcaaggg gcagagacgc
      601 tgccatttgg taccggctgg catccttatt ttccgttgtc gccgcaaacg cggattcagg
      661 cacaggcgag cggttactgg ctggagcggg agcagtggct ggcgggtgag ttttgcgagc
      721 agctaccgca ggagctggat tttaaccagc tcgcgccgtt gccgcgccag tgggtaaaca
      781 atggttttgc cggatggaat ggtaaggccc gcatcgagca gccgcaggag ggttatgcca
      841 tcatcatgga aacgacgcca cctgcacagt gttatttcat ctttgtttca gaccctgcgt
      901 ttgataaagg atatgcgttt gatttcttct gtctggaacc gatgagccat gcgccggatg
      961 accatcatcg tccggacggt ggtgacctca ttgcgcttgc accaggggaa tcaacaattt
     1021 cagagatgtc gttgcgggtt gcgttgctgt aatttataaa atgccggatg cacaggccta
     1081 cggggtaatt tcgattttgt agcactcggt gcacgatgcc tgatgcgacg cttgctgcgt
     1141 cttatcaggc ctacaatcgg tgttggagcg gtaggccgga taaggcgttc acgccacatc
     1201 cggcatcttt cctaacggcg atcgagagaa atcgcccccg gcccggtaat tgccaacagc
     1261 aggaatgcgc cagcgatact tacattcttc cagaaattaa tcatatttgg cccaaccgca
     1321 tcgccggtca tatcccagta atggtggcca atcaccgccg tacccagcgt gtagaaaata
     1381 aacagcaccg ccagcggacg ggtgaaaaag ccaagcacga ttaatatcgc ggcgggcact
     1441 tccataacta ccgcaataat cgctgccagc atcggcattg gtgcgcccag cgaggccata
     1501 tattggaccg taccgtcaaa gcccatcatt ttgggaaaac caaaaataat gaaaattaag
     1561 accacggcga tacgggcaat taataataaa acggggcggg cagtgccaaa atcaannnnn
     1621 nnnnnaatcc ctttgcaaga tgatgtgatt taaacgtaat acacactctg gtacctcgcc
     1681 acgcccaggc gttgagatat tcgtttctgg tataacggca taacgcagat ggctgacaat
     1741 gtgcttattg tttcctgccg aatgcgacat gcacgcctta tccgtctcac atgagtttgt
     1801 cagttcaata aattgcgatt ttcacctgtt tcatcgttct tttttacatg ttgtgttatg
     1861 ggccgctctt ttttgtcatt tcctgatatg caggataacc gtcaggaagc aggctcgaat
     1921 attcttattt ttcaacttgc accaacattc gattaacctc agactaacgc ctcctgacgg
     1981 gagggcctca tggttttnnn nnnnnnnctg gcttaaaggg attggtttaa cgccagaaac
     2041 catcggcctg ttattggggg cgggtcttgt tgcccgtttc ctcgggagtt tgctcatcgc
     2101 gccccgcgtc agcgatcctt cccgcctgat ttccgccttg cgcgtgctgg cactgctgac
     2161 acttctcttt gttgtcgcct tctgggcggg ggcgcacgta gcgtggctga tgctggtgat
     2221 gattggcttt aacctctttt tctcaccgct ggtaccgttg accgatgcac tggcgaatac
     2281 gtggcaaaag cagttcccgc ttgattacgg caaagtgaga ctgtggggct cggtggcgtt
     2341 tgtcattggc tcggcgctga cgggcaaact ggtcagtatg tttgattatc gggtgatcct
     2401 cgcgctgttg acgttgggcg tggcatccat gctgctcggc tttctcatcc gtccgacgat
     2461 tcagccacaa ggggcaagcc gccagcagga gagcaccggc tggtctgcgt ggttggcgtt
     2521 ggttcgccag aactggcgct ttctggcctg cgtttgttta ttgcaggggg cacatgcggc
     2581 ctattacggt tttagcgcca tttactggca ggcagctggt tactcggcct cggcggtggg
     2641 gtatttgtgg tcgctgggcg tggtggcgga agtcattatc tttgcgctga gtaataaact
     2701 tttccgccgt tgtagtgcac gcgatatgct gttgatctcg gcgatttgcg gcgtagtgcg
     2761 ctggggcatt atgggagcaa ctacggcgtt gccgtggttg atagtggtgc aaattctgca
     2821 ttgcggtacc ttcacggtct gccacctggc tgccatgcgc tatattgctg ctcgccaggg
     2881 tagcgaagtc atccgtttac aggcggttta ctctgccgtc gcgatgggcg gcagtatcgc
     2941 catcatgacc gttttcgccg gtttcctgta tcaatatctc ggccacggcg tgttctgggt
     3001 gatggcgctg gtggcgcttc cggcaatgtt tttgcgcccg aaagttgttc cctcatgctg
     3061 attcgagcat tttgcggatc tgctcctgtt gatgtgttgt caatgtcagg tccgtataga
     3121 tcagcggtgg tgaaaagagc ggtaacggca tggtataagg cgcaataatc agtgccgcgc
     3181 cgcgcggagc gcctgtctgc aaaaatgcct ttaccgacat atgtttaatg ttgagcggca
     3241 gtaacgttag ttcgcgtagc tgctgctcaa tctgcgcttc tcgctcgcta tcattcccgg
     3301 tcaatagaat aatctgtttc tcatgcaggt cgttttcctg cattagccag gcaccaaaaa
     3361 tcaccgcgac cagaccgctt tcctcatcag aaagatggac gccgtattca ctttcaaatc
     3421 cggccagcgc cgcgcgggtg gtgcgcacca ggcgtggata caggcgggcg aactcttccg
     3481 gcagggtgtt atcaataccg atggcaaaca aactgcgatt taacgcctgg gcgaggtggg
     3541 tataaagctg atcgcataac ccctgttcat cataaaaacg aacatttccc agctcacgaa
     3601 agtggtttac cagacgtttg atagacttac gcagttgcct gtcccgctga tgcgcatccc
     3661 gcagtggatc gggaacccgc aacatggaaa aaagtaatgc cataaacagt ggctcatcag
     3721 gcggtacagg ctggagcgcc cgacgctgcc agtggcgtcc aatttcctgc gctacctgaa
     3781 attcaaggca ggattccgcc cagcgacgtt ggagcggatt aaactgaggc gtaattccag
     3841 cgtggtgttg cagcaaacaa tattgcagat acagacacag gaaatgaata tcgcgcgttt
     3901 caaagcgttt ttgcagccgt cgagagcaga gattcactaa cgcttgtaga ttggtatcgt
     3961 cgtaaaagtt acgcgcaatt ccgcgccgct tcagttcact cttcagggcg ggggtgaatc
     4021 ggctggtaat aaatgacgga cacagacgga aaccacgtcg tagccagtga aataaacaca
     4081 aacgttggtt aagcactgta ccttcaaccc ggtagctgcc gtcataacca gtagtcagtg
     4141 tgagttgatg atagcgcagg atctcccgtc ctgtttcgct gatatcaaga ctggcaatat
     4201 catcatccac accattaagc tcgctgaaag ttgccgtggt ggcggtcaac cccggctgaa
     4261 agagcgtcag caagatctgg cagcggcgct ggggagccga aaggacagat ggtggagcaa
     4321 gcgtaggcat catctgtaga tctcccataa gcataattga taagaatagc taaagtttgc
     4381 tcagggaaat ggcgaaggga atgctaatca tcagaaatgt tggcaagcgt cacagaattt
     4441 ttctcatctt aaactgttct gaccagtgga ttagcatgtc ttaaggtggt tttgtgaatg
     4501 attatcgtgc tagcgatcat gggttagttc tgcggattca acgcgctaag gataaaatcc
     4561 accatctgtt cgcgataaac atcgccgtga cgatcagaaa aagcatcaat gtgttcgcca
     4621 tccgggatta ggatcaaccg ttttggctct tttgccaggc tatacaactt ttcgctgtgc
     4681 tgccatggga taacgtgatc agctttaccg tgaatgagta aaagcgggat cgggctgacg
     4741 ctggcgatgt aattttcgcc gctgtaactc tcatcaagta a
//
LOCUS       DAWDRC010000296         4781 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7684_length_4781_cov_2.028989, whole genome shotgun sequence.
ACCESSION   DAWDRC010000296 DAWDRC010000000
VERSION     DAWDRC010000296.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7684_length_4781_cov_2.028989"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1343..1352
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1972..1981
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1172 a         1190 c         1084 g         1315 t
ORIGIN      
        1 ctcaacgtct gatcggcatt caccagcgca gcacgcattt cagtggtttt cttggcatct
       61 ttcactttca cacgcatcac cagacgccaa cctttggtaa ccgggttata gcgcaccgtg
      121 ctttcaacta tctcaccatt atcaccaatg ctggtttgcg ctgtgaccgg ggtatcctct
      181 ggcagttttt tcatctcagc gccggtaaaa tcgaccacaa aggcgatagt accgtcaggc
      241 tggcgaatca ggttcgactg cttcacatcc cccgttgaac gacgcgtttg ttgcacccat
      301 gcgttatctg gcgcatgcag tttgtcttca tcacggctga aggtgatggt gtatttaaag
      361 ttcatctctt tacccggctc cggcaactga tccggcgtcc agtaagcgac gatgttatcg
      421 ttggtttcat cgttggttgg aatttccacc agctcaacgc tgcctttgcc ccactcccct
      481 ttcggagtca cccatgcgct tggacgaaga tcgtaacgat catcgagatc ttcaaagcgg
      541 gagaaatcac gaccacgctg caatagaccg aagccttgcg ggttttccat cgagaagctg
      601 ctgaccgcta aatgtttcgg gttattcaac ggacgccaga tccattcgcc attaccagca
      661 tggatagaca gaccgttaga gtcgtgcaac tccggacgat agttatttgc aggcgacggt
      721 tggttcggcc caaacaggaa catactggtt aacggtgcaa cccccagttt gccgacttta
      781 tcgcgcagat agattttcga ctgcacatcc acaaccgtgt cacgtcctgg cataactacg
      841 aatttgtaag cacctgtcgc gcgcggcgag tcaagcaatg cataaatggt taaacgttta
      901 tcagtcggtt ttggacgctc gatccagaac tctttgaagc gtggaaattc ttcacccgat
      961 ggcaaggcgg tatcaattgc caggccgcgt gcagaaaggc cataaacctg acctgcacca
     1021 atcacgcgga aatagctggc cccgagcatg ctgacgattt catcgttttt atctttgctg
     1081 ttgatcgggt aaagcacttt gaaaccggca aaaccaaggt cttttaccgt gtctttgtca
     1141 tgctgaacat cgccgaaagt gaaataatcc gggctgtatt tgattcgttt gactgcggtg
     1201 gcagtcactt catttatttt gaccggggta tcgaagtaca taccctgatg gtagaactcg
     1261 agtttgaatg gggtcttcag attgttccag tacgctttgt catgattaaa ctggatctgc
     1321 tgatagtccg cgtatttcat atnnnnnnnn nnggcaagtt gcttttgggc gcctcatagc
     1381 ctttcccggc taaggattga gcttgctttg cgacatcatc aatactgaaa gcccagcttg
     1441 aagatgtata cagggttaac attactgcag cactcaacca acgcattttc atcatttgta
     1501 gtttatgttt cataattagt aagcacttcc ccctttgtgt gcttatatcg atccgatcta
     1561 ttttaatgga taacctgcca ttccgacaac agaatcccgg tatcgttcag cacactggct
     1621 catggattaa gcacctgatt gcaaccgttt tcactttccg tgcttctcct aaagacagaa
     1681 gcatcgactt cgtgtagggt ttgccagcaa atttatttat caaccgtctt actgacgctt
     1741 ccattctggt tagtggtagc gatgatgcta tgcataaaaa ccttgaagcc tgcgcctggc
     1801 gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc ataacccgac agggataaga
     1861 cgaaaaatcc gagattagtt aaccatatat gaatccagta cccgcgcaac gtgaatattt
     1921 cctcgactcc atccgcgcct ggctgatgtt gttagggatc ccttttcata tnnnnnnnnn
     1981 natttcttta atctattcga gccatacatg gcatgtgaat agcgccgaac cgtcattgtg
     2041 gctgaccctt tttaatgact tcatccactc gttccgcatg caggtgtttt tcgttatatc
     2101 cgggtacttt tcctacatgc tttttttacg ctatcccttg aaaaaatggt ggaaagtacg
     2161 tgtcgaacgt gtagggatcc cgatgttaac agccatcccc ttactgacat tgccgcaatt
     2221 tattatgctg caatacgtca aagggaaagc ggaaagttgg cctgggctgt cattgtatga
     2281 caaatataat acgttggcct gggaattaat atcacacctg tggtttttac tggtgttagt
     2341 ggtcatgacg acgctgtgcg tatggatatt taaacgcatc agaaataatt tagaaaattc
     2401 tgataaaacg aataaaaaat tctcgatggt aaaattatcg gtgatttttt tgtgcctcgg
     2461 catcggttat gcggtaataa gaagaacgat ttttattgtg tatccaccca ttctgagtaa
     2521 cggcatgttc aattttattg tcatgcaaac gttattttat ttgccgttct ttatcctcgg
     2581 cgcactggct ttcattttcc ctcatcttaa agccttgttt accacgccgt ctcgtggctg
     2641 tacccttgca gcagcattgg cgtttgtcgc ttatttactc aaccagcgct atggcagtgg
     2701 cgatgcctgg atgtatgaaa ccgagtcggt gatcactatg gtcctcggtc tgtggatggt
     2761 gaatgtggtc ttctcctttg gccaccgttt gcttaacttc cagtcagcgc gggtgactta
     2821 ttttgttaac gcatcgctgt ttatctatct ggttcaccac ccgttaacgc tgtttttcgg
     2881 cgcatacatt acaccacaca tcacctccaa ctggcttggt tttctctgtg gcctgatatt
     2941 cgtagtaggg attgcgataa ttctgtatga aattcattta cgcatcccat tattgaagtt
     3001 tttgttctct ggtaaaccgg ttgttaagcg tgagaacgat aaagcaccag cccgttaagc
     3061 cgcgtttaca ataaccattc aacgggcaat atcgacgcca gtttgaccat aacccgcttc
     3121 cagaaactgg tggcgggttc ttttttgaga ataatctctt tctttgaatg acgatcaacc
     3181 cagttgatcc gtccccacct gtccagacgg agctgccagg ccgcatcata ctggctctga
     3241 ataaagcgtt tatcaattaa ctgtgccagc gtttcgctct ctatcacgaa gcccatttca
     3301 gtattgagca acgttgaacg cggatcgaaa ttgaaagagc cgataaacac cgttttacca
     3361 tcgatactaa aggttttagc atgcaggctg gcccctgaat taccggttat gccgcgatcg
     3421 tgtaacgtac tactttgttc acgcgtcggc ttgagttcat ataattccac cccatagcgg
     3481 agcaattttt tgcgccagcg cgcgtatcct gcatggacga cagcaacatc gttagcggca
     3541 agagaattgg ttaggatcgc aatctttacc ccttttctca ccatccgtaa gagttgcgcc
     3601 acacctgcac gtgtcggtac aaaataggaa gagataatat cgatgcgttc actgggtgag
     3661 cccatgatat cgaacaggcg ctgcggtaac agtgaatgcc gttttgcctt gccctcccct
     3721 ttcgccggat catcacttaa taaacgtgtt ttcgcccaga taagcgacaa cgttccatca
     3781 accagatgat ttataaacgg actggattcc attttgcgta aataacgatg cgtcatggca
     3841 tcgtcatgcc aggatgccgg taactcgatg cgatccgcca tttcaccttc cgggacatcc
     3901 agcacctgct gtaaggggga aaccgatttg caataccaat agcgggcgaa atcatcggca
     3961 acgtcctcta ccacgggtcc tattgccatg acatctaaat ccgaaaaaag tggctcttcc
     4021 cctgcaccaa aataggcatc accaatattt cgtcctccca ccagcgtcac cacgccatcg
     4081 acagtgaaac ttttattgtg catacggcga ttgagacggg aaaagtcggt gatataacca
     4141 agcggacgta gcaagcgaaa cgagaaagga ttaaaaagcc ggacttcaat gcgtggatgg
     4201 ctgtcaagca agcgtaaaat gttatcaagt ccgggcgtat tgttgtcgtc cagcaacaaa
     4261 cgtacgcgaa cgccacgctt tgctgcggct aacagggcgg aaaacagtaa ccgacccgac
     4321 atatcgtcct gccagatgta atactgaaca tccagtgtat gctctgccat ttcagccaga
     4381 cgataccggg cggcaaacgc atccagactt ttctccagag gaaaaagtcc acactgaccg
     4441 ggatgttgcg aacacagtgg cagcaccgcg ctcgccagct gaggcaaatc attcatctcc
     4501 ttgttgggta aggagtcttt cgtagaggtg ggcgttttct tcatcataac agacaaagta
     4561 tacctgttcg ggtaaagcgt gacgggtaat aaattctgaa acggttttta ctgcgatttc
     4621 agccgctgcc gcacgggggt aaccataaac cccagtactg attgcaggaa aagccactga
     4681 cgtatagctg tttgccgcca ccagtcgtag gctattgaga taggcatcct gcaaaagctg
     4741 gtcttcgttt tgttcaccac cacgccagac tggcccgacg g
//
LOCUS       DAWDRC010000297         4775 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7702_length_4775_cov_2.374153, whole genome shotgun sequence.
ACCESSION   DAWDRC010000297 DAWDRC010000000
VERSION     DAWDRC010000297.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7702_length_4775_cov_2.374153"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    948..957
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1162 a         1153 c         1186 g         1264 t
ORIGIN      
        1 cgccagttgg ctattccggt gcgattcagc tgctggtggg agccgatttt aacggcacgg
       61 tacttggcac gcgcgtgact gagcaccacg aaacgccagg gcttggcgat aaaatcgaac
      121 tgcgcctttc tgactggatc acccattttg cgggtaaaaa aatcagtggt gcggatgatg
      181 cgaactgggc ggtgaagaaa gatggtggtg atttcgacca gttcaccggc gcgacgatta
      241 ctccccgcgc ggtggttaat gcggtaaaac gcgccggatt gtacgctcag acgttaccgg
      301 cacaactttc tcaacttcct gcctgtggag aataaaacgt gagcgaaatt aaagacgtta
      361 ttgttcaggg gttgtggaaa aacaactctg cgctggtcca gttgctcggc ctttgtccgc
      421 tgttggcggt cacgtccact gccactaacg ctctgggttt aggacttgcg actacgctgg
      481 tactgacgct aaccaacctg accatttcga cgctgcgtca ctggacgccc gccgagatcc
      541 gcattcccat ttacgtgatg atcatcgcct cggtggtcag cgctgtacag atgctgatca
      601 acgcctacgc ctttggcctg tatcaatcat tagggatttt tattccgctg attgtcacta
      661 actgtatcgt tgtgggccgc gctgaagcct tcgccgccaa aaaaggtccg gcgctttcgg
      721 cactggacgg cttttcaatt ggtatgggcg caacctgcgc catgttcgtg ctgggttcac
      781 tacgcgaaat tatcggcaat ggcacgttgt ttgacggtgc agatgcgctg ttaggtagct
      841 gggcgaaagt attacgcgtg gagattttcc acaccgactc ccctttcctg ctggcgatgc
      901 tgccaccagg tgcatttatt ggcctgggac tgatgctggc aggaaaannn nnnnnnngaa
      961 aatacctgat tgatgaaaaa atgaaaaagc gccgtactga agcagttgcc gaacgtgcat
     1021 tgccaaacgg tgaaacaggg aatgtctgat gaacaaagca aaacgcctgg agatcctcac
     1081 tcgcctgcgt gagaacaatc ctcatcccac caccgagctt aatttcagtt cgccttttga
     1141 attgctgatt gccgtactgc tttccgctca ggcgaccgat gtcagtgtta ataaggcgac
     1201 ggcgaaactc tacccggtgg ctaatacgcc tgcagcgatg cttgaactgg gcgttgaagg
     1261 ggtgaaaacc tatatcaaaa cgattggcct ttataacagc aaagcagaaa atatcatcaa
     1321 aacctgccgt atattactgg aacaacataa tggcgaggtt ccggaagatc gtgctgcgct
     1381 tgaagccctg cctggtgtag gtcgtaaaac agccaacgtc gtattaaaca ctgcattcgg
     1441 ctggccgact attgctgtcg acacgcacat tttccgcgtt tgtaatcgta ctcaatttgc
     1501 gccggggaaa aacgtcgaac aggtagaaga aaagctactg aaagtggttc cggcagagtt
     1561 taaagtcgac tgccaccatt ggttgatcct gcacgggcgt tatacctgca ttgcccgcaa
     1621 gcctcgctgt ggctcttgta ttattgaaga tctttgtgaa tacaaagaga aagttgacat
     1681 ctaaagatca ggggtaacac cgattacccc attcataacc tttctttatc ctcttttaaa
     1741 acatcttttt aacgtcaatg atgccattgc ttagcgttat catcaggtaa tccgtttgca
     1801 gataaccaaa aatgcaggtt aattgttttt ttaatagcga aattttctat tcattcgtga
     1861 tcaagatcac gatgctgtgg caatgtaaaa acattgcaag aatcaggtta aaatcattta
     1921 aatttacaca cgcaacaaat attgacctac aaaacattac actggctatt tttcagaaac
     1981 tggactatct cactaggaaa aacgccaaat agtagaacat atcgccatta agccatcatc
     2041 accctctcag ggtagtgaaa aaatctgccg ttacgttttt tgaaaaattt aacgctggat
     2101 aacatttccc ggaatggttg aattccccgc ctcagttata tgtaacagat tattacaaag
     2161 gacttgtctg aaagtgcaag atagtgaaca ttacctgccg tttcccctcc cactataaca
     2221 attgcgcgta tgttttttag acataacgcg agaaagcacc cccgttaata tgggatgtaa
     2281 aaaaagaggt aaaagtgtcc actgcaaacc aaaaaccaac tgaaagcgtc agtttgaacg
     2341 cttttaaaca accgaaggcg ttctatctca tcttctcgat tgagttatgg gaacgttttg
     2401 gttattacgg cctacaagga attatggctg tttacctggt taaacaactg ggtatgtctg
     2461 aagcggattc aatcaccctt ttctcttcct ttagtgccct ggtttatggt ctggtcgcta
     2521 tcggcggctg gttaggtgac aaggtactgg gtactaaacg cgtaattatg ctcggcgcta
     2581 ttgtgctggc gattggttat gctctggttg cctggtctgg tcacgacgcc ggtatcgttt
     2641 atatgggtat ggcggctatt gcggtcggta acggcctgtt taaagctaac ccgtcttctc
     2701 tgctttctac atgctatgag aaaaacgacc cgcgtctgga cggtgcattc accatgtact
     2761 acatgtccgt caacatcggc tctttcttct ctatgattgc tacgccgtgg ctggccgcga
     2821 aatacggctg gagtgttgcg tttgcgttga gcgttgtagg cctgctgatc actatcgtta
     2881 acttcgcctt ctgccaacgt tgggttaaac agtacggttc aaaaccagac ttcgagccta
     2941 tcaactaccg taacctgctg ctgaccatta ttggtgttgt ggcactgatc gctatcgcca
     3001 cctggctgct gcacaatcag gaagttgcgc gtatggcgct gggcgttgtt gccttcggga
     3061 tcgtggttat cttcggtaaa gaagccttcg cgatgaaagg tgctgcgcgt cgtaaaatga
     3121 tcgttgcctt catcctgatg ctcgaagcca ttatcttctt cgtgctttat agccagatgc
     3181 caacgtcact gaacttcttt gcgattcgta acgttgaaca caccattctg ggcctggccg
     3241 tagaacctga gcagtatcag gcactgaacc cgttctggat catcattggt agcccgattc
     3301 tggccgctat ctataacaag atgggcgata ccctgccaat gccaaccaag tttgctatcg
     3361 gcatggtgat gtgttctggt gcgttcctga ttctgccgct gggtgcgaaa ttcgcgtctg
     3421 acgcaggtat cgtgtctgta agctggctgg tcgcaagcta tggcctgcag agtatcgggg
     3481 aactgatgat ctctggtctg ggtctggcaa tggttgcaca actcgttccg cagcgtctga
     3541 tgggcttcat tatgggtagc tggttcctga ccactgccgg tgcaaacctg attggtggtt
     3601 atgttgcggg tatgatggct gtgccggata acgttaccga tccactgatg tcactggaag
     3661 tctatggtcg cgtattcttg cagattggtg ttgctactgc cgttattgca gtactgatgc
     3721 tgctgaccgc gccgaaactg caccgcatga cgcaggatga cgctgcagac aaagcggcga
     3781 aagctgctgt agcgtaaaat tcagggaaac tcttttacaa gccgctaact tttcgttagc
     3841 ggcttttttt ttgttcagca acgcactacc atactttaaa ccacagacaa aaggagttac
     3901 cgatgaaatt gttctacaaa ccgggcgcct gctctctcgc ttcccatatc accctgcgtg
     3961 agagcggaaa ggattttacg ctcgtcagtg tggatttaat gaaaaaacgt ctcgaaaacg
     4021 gtgacgatta ctttgccgtt aaccctaagg gacaggtgcc tgcattgctg ctggacgatg
     4081 gtactttgct gacggaaggc gtagcaatta tgcagtatct tgccgacagc gtccccgacc
     4141 gccagttgct ggcaccggta aacagtattt cccgctataa aaccatcgaa tggctgaatt
     4201 acatcgccac cgagctgcat aaaggcttca cacctctgtt tcgccctgat acaccggaag
     4261 agtacaaacc aacagttcgc gcgcagctgg agaagaagct gcaatatgtg aacgaggcac
     4321 tgaaggatga gcactggatc tgcgggcaac gatttacaat tgctgatgcc tatctgttta
     4381 cggttctgcg ctgggcatac gcggtgaaac tgaatctgga agggttagag cacattgcgg
     4441 catttatgca acgtatggct gaacgtccgg aagtacaaga cgcgctgtca gcggaaggct
     4501 taaagtaata gccatcggcc cacaggtcgt tgtgggccga aatgagatat ttcagagctt
     4561 tgttgcgctg aaataatgtt ctggtttggc aatacgatcc tgagcagcaa ccacttgcag
     4621 ctcatattcc tgcattgctt ttgtggtcac catgatttcg tatactgcag cggtcacatg
     4681 ttccagggcc tcctgcagcg ttgccccctg aagcagtttc accagcagta aaccgctcgt
     4741 cacatcacca acacctaccg gctggcgcat accaa
//
LOCUS       DAWDRC010000298         4764 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7710_length_4764_cov_2.532385, whole genome shotgun sequence.
ACCESSION   DAWDRC010000298 DAWDRC010000000
VERSION     DAWDRC010000298.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7710_length_4764_cov_2.532385"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          979 a         1164 c         1417 g         1204 t
ORIGIN      
        1 gtgcggcaac acactttata cggcaggcaa tctcaacaac gacatcggtg taccgatgac
       61 gctgttgcgc ttaacgccgg aatacgatta cgcagttatt gaacttggcg cgaaccatca
      121 gggcgaaatt gcctggactg tgagtctgac tcgcccggaa gcggcgctgg tcaacaacct
      181 ggcagcggcg catctggaag gttttggcta gcttgcgggt gtcgcgaaag cgaaaggtga
      241 aatctttagc ggcctgccgg aaaacggtat cgccattatg aacgccgaca acaacgactg
      301 gttgaactgg cagagcgtaa ttggctcacg caaagtgtgg cgtttctcac ctaatgccgc
      361 caacagcgat ttcaccgcca ccaatatcca tgtaacctcg cacggtacgg aatttaccct
      421 gcaaacccct acaggtagcg tcgatgttct gctgccgttg ccggggcgtc acaatattgc
      481 gaatgcgctg gcagccgctg cgctctccat gtccgtgggc gcaacgcttg atgctatcaa
      541 agcggggctg gcaaatctga aagctgttcc aggccgtctg ttccccataa aactggcaga
      601 aaaccagttg ctgctcgacg actcctacaa cgccaatgtc ggttcaatga ccgccgctgt
      661 tcaggttctg gctgaaatgc cgggctaccg cgtgctggtg gtgggcgata tggcggaact
      721 gggcgctgaa agcgaagcct gccatgcaca ggtgggcgaa gcggcaaaag ctgctggtat
      781 tgactgcgtg ttaagtgtgg gtaaacaaag ccatgctatc agcactgcca gcggcgttgg
      841 cgaacatttt tccgataaaa ctgcgcttat cgcgcgtctt aaatcactga ttgctgagca
      901 acaggtaatt acgattttag ttaagggttc acgtagtgct gccatggaag aggtagtacg
      961 cgctttacag gagaatggga catgttagtt tggctggccg aacatttggt caaatattat
     1021 tccggcttta acgtcttttc ctatctgacg tttcgcgcca tcgtcagcct gctgaccgcg
     1081 ctgttcatct cattgtggat gggcccgcgt atgattgctc atttgcaaaa actttccttt
     1141 ggtcaggtgg tgcgtaacga cggtcctgaa tcacacttca gcaagcgcgg tacaccgacc
     1201 atgggcggga ttatgatcct gacggcgatt gtgatctccg tactgctgtg ggcttacccc
     1261 tccaatccgt acgtctggtg cgtactggtg gtgctggtag gttacggtgt tattggcttt
     1321 gttgatgatt atcgcaaagt ggtgcgtaaa gacaccaagg gcctgattgc ccgttggaag
     1381 tatttctgga tgtcggtcat tgcactgggt gttgccttcg cgctgtacct tgccggcaaa
     1441 gacacgcccg caacgcagct ggtggttccg ttctttaaag atgtgatgcc gcaactgggg
     1501 ctgttctaca ttctgctggc ttactttgtc attgtgggta ctggcaacgc ggtaaacctg
     1561 accgatggtc tcgacggtct ggcaattatg ccgaccgtat ttgtcgccgg tggttttgcg
     1621 ctggtggcgt gggcgaccgg caacatgaac tttgccagct atttacacat accgtatcta
     1681 cgacacgccg gggaactggt cattgtctgt accgcgatag tcggggcagg gctgggcttc
     1741 ctgtggttta acacctatcc ggcgcaggtc tttatgggcg atgtaggttc gctggcgtta
     1801 ggtggtgcgt taggtattat cgccgtactg ctgcgtcagg aattcctgct ggtgattatg
     1861 gggggcgtgt tcgtggtaga aacgctttcc gtcatcctgc aggtcggctc ctttaaactg
     1921 cgcggacaac gtattttccg catggcaccg attcatcacc actatgaact gaaaggctgg
     1981 ccggaaccgc gcgtcattgt gcgtttctgg attatttcgc tgatgctggt cctgattggt
     2041 ctggcaacgc tgaaggtacg ttaatcatgg ctgattacca gggtaaaaat gtcgtcatta
     2101 tcggcctggg cctcaccggg ctttcttgcg tggacttttt cctcgctcgc ggtgtgacgc
     2161 cgcgcgttat ggatacgcgt atgacaccgc ctggcctgga taaattaccc gaagccgtag
     2221 aacgccacac gggcggtctg aatgatgaat ggctgatggc ggcagatctg attgtcgcca
     2281 gtcccggtat tgcactggcg catccatcct taagcgctgc cgctgatgcc ggaatcgaaa
     2341 tcgttggcga tatcgagctg ttctgtcgtg aagcacaagc accgattgtg gcgattaccg
     2401 gttctaacgg caaaagcacg gtcaccacgc tagtgggtga aatggcgaaa gcggcggggg
     2461 ttaacgttgg tgtgggtggc aatattggcc tgcctgcgtt gatgctgttg gatgatgagt
     2521 gcgaactgta cgtgctggaa ctgtcgagct tccagctgga aaccacctcc agcttacagg
     2581 cggtagcagc gaccattctg aacgtgactg aagatcatat ggatcgctat ccgtttggtt
     2641 tacaacagta tcgtgcagca aaactgcgca tttacgaaaa cgcaaaagtt tgcgtggtta
     2701 atgctgatga tgccttaaca atgccgattc gcggtgcgga tgaacgctgc gtcagctttg
     2761 gcgtcaacat gggcgactat cacctgaatc atcagcaggg cgaaacctgg ctgcgggtga
     2821 aaggcgagaa agtgctgaac gtgaaagaaa tgaaactttc cgggcagcat aactacacca
     2881 atgcgctggc ggcgctggcg ctggcagatg ccgcagggtt accgcgtgcc agcagcctga
     2941 aagcgttaac cacattcact ggtctgccgc atcgctttga agttgtgctg gagcataacg
     3001 gtgtgcgctg ggttaacgat tcaaaagcga ccaacgtcgg cagtacggaa gcggcgctga
     3061 atggcctgca cgtagacggc acgctgcatt tgttgctggg gggcgatggt aaatcggcgg
     3121 actttagccc actggcgcgt tacctgaatg gcgataatgt acgtctgtat tgtttcggtc
     3181 gggacggcgc gcagctggcg gcgctacgcc cggaagtggc agaacaaacc gaaaccatgg
     3241 aacaggcgat gcgcttgctg gctccgcgtg ttcagccggg cgatatggtt ctgctctccc
     3301 cggcctgtgc cagccttgat caattcaaga actttgaaca acgaggcaat gagtttgccc
     3361 gtctggcgaa ggagttaggt tgatgcgttt atctctccct cgcctgaaaa tgccgcgcct
     3421 gccaggattc agtattctgg tctggatctc cacggcgcta aagggctggg tgatgggctc
     3481 gcgggaaaaa gataccgaca gcctgatcat gtacgatcgc accttactgt ggctgacctt
     3541 cggcctcgcg gcgattggct ttatcatggt gacctcggcg tcaatgccca tagggcaacg
     3601 cttaaccaac gatccgttct tcttcgcgaa gcgtgatggc gtctatctga tcctggcatt
     3661 cattctggcg atcattacgc tgcgtctgcc gatggagttc tggcaacgct acagtgccac
     3721 gatgctgctc ggatctatca tcctgctgat gatcgttctg gtagtgggta gctcggttaa
     3781 aggggcatcg cgttggatcg atctcggttt gctgcgtatc cagcctgcgg agctgacaaa
     3841 actgtcgctg ttttgctata tcgccaacta tctggtgcgt aaaggcgacg aagtacgtaa
     3901 taacctgcgc ggcttcctga aaccgatggg cgtaattctg gtgttggcag tgttactgct
     3961 ggcacagcca gaccttggta cggtggtggt gttgtttgtg actacgctgg cgatgttgtt
     4021 cctggcagga gcgaaattgt ggcagttcat tgccattatc ggtatgggca tttcagcggt
     4081 tgtgttgctg atcctcgccg aaccgtaccg tatccgccgt gttaccgcat tctggaaccc
     4141 gtgggaagat ccctttggca gcggctatca gttaacgcaa tcgttgatgg cgtttggtcg
     4201 cggtgaactg tggggacaag gtttaggtaa ctcggtacaa aaactggagt atctgccgga
     4261 agcgcatact gactttattt tcgccattat cggcgaagaa ctggggtatg tcggtgtggt
     4321 gctggcactt ttaatggtat tcttcgtcgc ttttcgcgcg atgtcgattg gccgcaaagc
     4381 attagaaatt gaccaccgtt tttccggttt tctcgcctgt tctattggta tctggtttag
     4441 tttccaggcg ctggttaacg taggcgcggc ggcggggatg ttaccgacca aaggtctgac
     4501 attgccgctg atcagttacg gtggttcgag cttactgatt atgtcgacag ccatcatgat
     4561 gctgttgcgt attgattatg aaacgcgtct ggagaaagcg caggcgtttg tacgaggttc
     4621 acgatgagtg gtcaagggaa gcgattaatg gtgatggcag gcggaaccgg tggacatgta
     4681 ttcccggggc tggcggttgc gcaccatcta atggctcagg gttggcaagt tcgctggctg
     4741 gggactgccg accgtatgga agcg
//
LOCUS       DAWDRC010000299         4752 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7729_length_4752_cov_2.176709, whole genome shotgun sequence.
ACCESSION   DAWDRC010000299 DAWDRC010000000
VERSION     DAWDRC010000299.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7729_length_4752_cov_2.176709"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    843..852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1651..1660
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1191 a         1086 c         1183 g         1272 t
ORIGIN      
        1 tcatgtcaga taaaaatgag atagtagtca cattttcttg cactttattc cagccagttc
       61 ataagtattt ccgtaaaaag aacagctatt tgaaacacct gagggtttgc tgttgaaacg
      121 ccgtcttatt attgctgctt ctttgttcgt ttttaattta tcgtctggtt ttgcggcgga
      181 aaacattcct ttttcacctc agcctccaga gattcatgcc gggtcctggg tattgatgga
      241 ttacaccacc ggtcagattc tcaccgcggg taatgagcat caacagcgca atcccgccag
      301 cctgacaaag ctgatgacgg gctatgtcgt ggatcgcgct atcgatagtc atcgcattac
      361 gccagacgat attgtcaccg tggggcgtga tgcgtgggcg aaagataatc cggtgtttgt
      421 cggttcttca ctgatgtttt tgaaagaggg cgatcgcgta tcggtacgtg atttaagccg
      481 tggtttaatt gtggattccg gaaatgacgc ttgtgttgct ctggctgact atattgccgg
      541 tgggcaacgg cagtttgttg aaatgatgaa caactatgcc gagaagctgc atctcaagga
      601 tacgcatttt gaaacagtgc atggtctgga tgcacctggc cagcatagct cggcttatga
      661 tttagccgtg ctttctcgcg ctatcatcca cggcgagccc gagttttatc atatgtacag
      721 tgagaaaagc ctcacctgga acggtatcac ccagcaaaac cgtaacgggt tattgtgggc
      781 taaaaccatg aatgttgacg gcctgaaaac gggccatact tctggtgccg ggtttaatct
      841 tannnnnnnn nntcggctgt agacgggcag cgtcgcctca ttgcagtggt aatgggtgct
      901 gacagtgcaa aaggtcgtga ggaagaggca agaaaattac tgcgttgggg gcaacaaaac
      961 tttactacgg tgcaaatttt gcaccgtggg aaaaaggtcg gtacggaacg catctggtat
     1021 ggcgataaag aaaatatcgc cctgggaacg gaacaagagt tctggatggt gctaccgaaa
     1081 gccgaaattc cacatatcaa agccaaatat acccttgatg gtaaagaact caccgcgcca
     1141 attagcgccc atcagcgggt aggggaaatt gaactttacg accgtgataa acaggtggcg
     1201 cactggccgc tggttaccct ggaatctgtc ggggaaggca gcatgttttc ccggctgagt
     1261 gattatttcc accataagga ctgacctttc ttttgcagca gactggcagg agtgcgaatc
     1321 tgctcgcata atcaacactc attccttgtg gttttaatat tgcaactata ctgtatataa
     1381 aaacagtatt aatgggggcg tcatgaacta cgagattaag caggaagata aacgtaccgt
     1441 tgcaggtttc catctcgttg gcccgtggga acagacggta aagaaaggct ttgagcagtt
     1501 gatgatgtgg atagatagca aaaatattgt gccgaaggag tgggttgctg tctattacga
     1561 caatccagat gaaacacccg ccgaaaaatt acgctgcgac accgtcgtga cggtaccgaa
     1621 taactttacg ctccccgaaa acagtgaggg nnnnnnnnnn gggcgtcatt ctgacagaaa
     1681 tttcaggtgg tcagtatgcg gtggcggtgg ctcgtgtagt cggtgatgat tttgctaaac
     1741 cctggtatca gttctttaat agcctcttgc aggacagtgc ttatgaaatg ttaccaaagc
     1801 cctgcttcga ggtttacttg aacaatggcg cggaagatgg gtactgggat atcgaaatgt
     1861 atgttgcggt gcagccaaaa catcactaat tcatctcagg gcggtgtgtt aacgcgaaga
     1921 ccactctttt tttgaaagcg aaaagagtaa gatgcgcctt tcaaattttt cgctcctgcc
     1981 gggaaattac actgttcccg gtatgtccgt cggataactc agaggcgcgc cttctggccg
     2041 acagatgtgt tatgagcgct tttaatcttc attacggagt ttctgcgtgc gtgccgataa
     2101 gtcattaagc ccgtttgaaa tccgggtata ccgccattac cgcattgtgc atggtactcg
     2161 ggtcgcgctg gcattcctgc tcacttttct cattatccgc ctgtttacta tcccggaaag
     2221 cacctggccg ctggtcacca tggtggtgat tatggggcca atctcgttct gggggaacgt
     2281 tgtccctcgt gccttcgaac gtattggcgg tacggtgtta ggttcgattt taggtcttat
     2341 cgctctgcaa ctggagttaa tttcgttacc gctgatgtta gtctggtgcg cggcggcgat
     2401 gtttctttgc ggttggctgg cgctgggcaa gaaaccgtat caaggattat tgattggggt
     2461 gacgctggca attgttgtgg gttccccgac aggtgaaatt gatacggcgt tatggcgaag
     2521 cggcgatgtg atcctcggct ctttactggc aatgttgttt accggtatct ggccacaacg
     2581 ggcgttcatc cactggcgca ttcaactggc gaaaagtctg accgagtata atcgggtcta
     2641 tcaatctgca ttctcaccga acttactcga acgcccacgt ctggaaagcc atctacaaaa
     2701 actgctgacc gatgccgtga aaatgcgtgg gctgattgcg cccgccagca aagaaacccg
     2761 tattcccaaa tcgatatatg aaggtatcca gaccattaat cgcaatctgg tttgtatgct
     2821 ggagttgcaa atcaatgcat actgggccac gcgccccagc catttcgtgt tattgaacgc
     2881 gcaaaaactt cgtgataccc agcacatgat gcagcaaata ctgctgagcc ttgttcatgc
     2941 gctgtacgaa ggtaatccgc agccggtttt tgccaatacg gaaaaattga acgatgctgt
     3001 ggaagagctg cgtcagttgc tcgataacca ccatgatctg aaagtcgtgg aaacaccaat
     3061 ctatggttat gtgtggctga acatggaaac ggcacatcag cttgagttgc tatcgaatct
     3121 gatttgccgg gccttgcgca aataattcct gaacttcaga atcatcttgc tgctgcttcg
     3181 attcagcaag gataaagggt atgatagtga aaagggataa aagcattgtc atctgcggca
     3241 gctatgagta atgttggccc taacgaatag cggttgctta aacgaatccg actctcacat
     3301 tatcaggggt ataaaaatgg aaactaccaa gccttcattc caggacgtac tggaatttgt
     3361 tcgtctgctc cgtcgtaaga acaaactgca acgtgaaatt caggacgttg agaaaaagat
     3421 ccgtgacaac cagaagcgcg tcctgttgct ggacaacctg agcgattaca tcaagccagg
     3481 gatgagcgtt gaagcaatcc agggcatcat cgccagcatg aaaggtgact atgaagatcg
     3541 cgttgacgat tacatcatca aaaatgccga gctctccaaa gaacgccgcg atatctccaa
     3601 aaagctgaaa gctatgggcg aaatgaaaaa cggcgaagcg aagtaattcc cgttttattc
     3661 aatgagggtt gcccggcaac cctcattgct cattgattct tatctgtgta tcaccgtcat
     3721 cattctcatc tgagaaccaa tcgaaattaa caacagcctt cttctgtatg cagcaaggca
     3781 aaaagttctg taactccatt gttattaact gcactggtta ctaacacgtt gtgcgctcca
     3841 gcttcccgta accaactttt caccaaagat atttgttcca tgctggctaa atctgctttg
     3901 gttactactc caatgaccgg gtgattcatg gcccggtagg gcgtttttac ctctaattcc
     3961 tgcttcagcg ctgagattcc tgcttgttgc cggaaaagca ggcacctgcc gtctgaacgg
     4021 tatcgatccg gaagcgtatc tgcgccatat tctgagcata ctgccggaat ggccctccaa
     4081 ccgtgttgac gaactcctgc catggaacgt agttctcacc aataaataag cgtcaatacg
     4141 gtgctccgtt gacgcttact ttaatccgtt ttctgagctt agatttttct gttcctaccc
     4201 agaccgtgga tttggaattc attttttgtc aggtcaatgc taatcgttaa aacggccata
     4261 acgcacctcc actggaaggt ttatcagtaa tatcctaccg ttgcttcatg gtcggggcgt
     4321 ccattccatt attatcggtt ttcctttctg tttataaatg aaaacgccag ctgtattcag
     4381 gctggcgtca ggggaaatga agcctgttga gggagattca ccggttctgg tgcagaagct
     4441 gcagatggcg cagggtaagg acatcttctc ttgcctgctt aatacaggac aacagtgctg
     4501 tttctgtggt taatggcact ataccctgta tcactgcatt gtcttacagc aactggtgag
     4561 ggctggcata agttgccggt gtttcggtag agtcacggcc atccaccacg cggagtgaat
     4621 aaccgttttc agcggcctta ttaatcagtt cagtgatatt aacaccatca atgtcaacga
     4681 caatgtgccc catatccagc gcctgtacgt taacgctgtc ggcttccagt gtcatcattt
     4741 cgcctccgga aa
//
LOCUS       DAWDRC010000300         4748 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7740_length_4748_cov_1.910292, whole genome shotgun sequence.
ACCESSION   DAWDRC010000300 DAWDRC010000000
VERSION     DAWDRC010000300.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7740_length_4748_cov_1.910292"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    532..541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2191..2200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3177..3186
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4167..4176
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1213 a         1166 c         1167 g         1162 t
ORIGIN      
        1 taaaactgga taaacagttt tttcatcgct tattcccacg catgaggcgc aaaaagatag
       61 cctttgttac gcacggtttt aatacgataa ggttctgcgg cgttatcgag cagttttttt
      121 cttaaccgcg aaatagccac gtccacgcta cgatccagac cgtcataact gacgccgcgt
      181 aaatttttca gcaatgcatc gcggtccatg atttgcccgg catgggtagc taattcccac
      241 aataattcga aatcagctgt cgagagcgag atttcagtgt tagccagggt gactacgcgg
      301 ttgatgggat cgatggtcaa cgtgccgaaa tgcagggctt tgtagggagt cagagacgtt
      361 tcctgaatac ctttggtcac tgtggcttgc tcattctgac gcaaatgcaa acgtaaacgc
      421 gctagcaaaa cagcaggggg cgtcgttttg agaatatagt cgcaggcgcc catttccagt
      481 gccaggatgt ggttcatatc gctatcgaga gaggttagaa gaacaatcgg tnnnnnnnnn
      541 nactttgcgc gtaaatcacg acaaatggtc atgccgtcct tgcctggtag catgatgtcg
      601 agtaacacca aatccggatt ttctcgcaga atagtttctt cggcccggtc gccgcgcggc
      661 tctacggtaa cctgcatatc atgttttgcc aggtatgcgg caatcagtgc accgacttcc
      721 gcatcatctt ccacaaatac gatagtgttc ataacattca cggtagatat aaaaacgtca
      781 caatacaccg cactgttttt acttactatc aatcgtttct aaacaaggtt tcatcaggta
      841 ttctactgat gacttgttat ggaagtgtta aggtaaaaag atggggctgg taatcaaagc
      901 cgcgctcggt gcgctggtag tgctgttgat tggtgtttta gcaaaaacga aaaattatta
      961 tatcgccggg ctgattccac ttttcccgac ctttgcgctt atcgcgcatt atattgttgc
     1021 cagcgaacgc ggcattgaag ccttacgcgc aaccatcatt tttagtatgt ggtcgattat
     1081 tccctatttt gtttacctgg cgtcgctgtg gtatttcacc ggaatgatgc gcttgcccgc
     1141 cgcttttgtt ggttctgttg cgtgttgggg gataagcgca tgggtgttga ttatatgctg
     1201 gataaagctg cattaacgca gatgacgacc gccatcaaga gggaaactgc gtccggtgac
     1261 aaagcagctg gtaagtaagt aatcgaccag gtcgatcact tctttctcgc caggcgcggt
     1321 tttcatcagt gatttattca gcgcctgttg tcgatattcg gcatcatcat gttcattaaa
     1381 caggatcagc gatggcgcaa tagaattcac tttcacttcc ggtgccagct tgcgggcaaa
     1441 cgagcgggtc atattatcca gtgccgcttt gcttgcagca taggcaatat gtttgtcgct
     1501 accgcgctcc accacataat cggtaaagtg aatgatatcg ctggcggcgt gtccgtgccc
     1561 acgcagtaat ctttccagag catggttgag caggtatggg gtattaacgt ggatctgcat
     1621 catgcaagcc agtacgtcgg tcagtggcgc acccggtttt tccgccatcc acgcgctggc
     1681 gttatgcaaa atagcacgca gaccatgggt gctttttagt acttcatcgg caaacgccat
     1741 cacaccgtcg ttggtcgaaa aatcagcctg aatacactgc gcacctgctt taatcagtcc
     1801 atcaatggcg ggatagtgcg tccgatagct gacaatcacc ggttgctttt gattaatgaa
     1861 atgccatgcg agggcgaggc cgatgcggcg acctccgcca gtaattaata ttggcaaggg
     1921 ctgggtttta cccatcgtga tctcctttgc tatccaacga tgggatgaac gttatcccac
     1981 cagcatccag gtcgccggaa ctgaggcaat taacagcatg ccaatcagca ccatttcctg
     2041 acggttcagt acattatcat gtgtatgcgt tttacgcgcg tacaggaaaa ccagcagccc
     2101 cggcgcatac agaacaacgg ataacagcaa gtgcattgga ccagaggcgt acagtaacca
     2161 taagccataa atgcaggcac cgacacctac nnnnnnnnnn gtggacgggt tgcgattttc
     2221 agcaagaacg cgccgaccag gaaatagggc accagaatca tttctgaggc gatggtcagc
     2281 aatgtgttgt aatcggaacc ggtcagccag atgagcacca gacaaatttg cacgcagatg
     2341 ttggtcagcc acagtgaagc ggatggcgca gcttgtgcgt tctggcgcgc aaaaatgcgc
     2401 gggaatgctt tatgagtggc tgcaaggaac ggaactgtct ctgccatgat ggtccagctt
     2461 aagtacgcgc cgcaaacgga aacgatcaaa ccggcagcga tgatgatttc gccccacggt
     2521 cccatcattt ccaccatcag accggccatt gacgggttac gaatttcagc cagttcagga
     2581 cgtgccacca cgcccagcga aagcagcgtt accagcaaat aaacgcccag agcggagaga
     2641 accgccagca gtgtcgcttt gccaacatca cgcttattac gcgcacgcgc agaaacaacc
     2701 acggcacctt ccacaccaat gaaaacccac agggtgatca gcatggtgtt tttcacctgt
     2761 tcccacacgg gtacgccaag tgcaagtccg gtgaagtcga gcttgaaggt atccagtttg
     2821 aacatcatta tcgccagcac aacaaacaga cccaacggca acaattttgc cagtgtcgcc
     2881 acaaggttaa tgctggcagc ggtttgcacc ccgcgcagga tcaaaaagtg aacaatccac
     2941 aataaagccg atgcaccgac gatcgattgc caggtattac catcgccaaa caggcgcaat
     3001 tccggcgtgt ccgtaaaaaa gcttaacgcg gaaaaaacga tcaccagata ggagacgttg
     3061 gcgatgaccg cgcacagcca gtatccccat gcggaacaaa agccgattag ctcgccaaac
     3121 ccttcgcggg cataggtaaa gataccgccg tcaagttcgg ggcgaatgcg cgtgagnnnn
     3181 nnnnnngcgt gaggatcagc atggcaaagg ccagcaataa aatgccagcg ccagtaatac
     3241 cccagccgat gagcagtgct gccgggctgg caactgccgc catattttgc ggcagactga
     3301 aaacacccgc gcccagcatt gagcttaata ccagcgcggt gagtgcgctc agtcccagtt
     3361 tcttttccat cgatatccag tgattaaaac gtgacaagaa gaataaatat tagggcaaaa
     3421 cgcataaaaa tggagtttgc cactgaagcg cgggatttta cgaagagatg tgtctgcatg
     3481 caatggtcgc agggaaattt atgactaaaa atgcgaaagg cggcatcaag ccgccttata
     3541 ggagtaactg aatagttatt tatacagatc tgcgctgacg gtcaggttat taccacgttc
     3601 ctgccactgg cgggtgatgt ggtaatactt agcacctttc ttcgcggcac gtttcgcaac
     3661 ctgataggag acttcggtca tgttgccgta gttgccagag aatttgatgc tgtcgaacgg
     3721 gaccatcatc gctgctgtcg ctttgttcag ttcttcgact ttagtgccat ccgggagcgt
     3781 gacggtgtaa cgcccgcctt ttgatgactg ggtttcaaag aagcgaccga cttcagaact
     3841 tggtgatgcg gtagtcgcaa cacccgggat ctcaactttc ttcgcggctt cgccaccggc
     3901 agccagagct gcacgtcctg cttcggaatc tgccgggatt acatccgggc tctggacgat
     3961 acgtttctta gcatcttttt tatagatgaa tgcagtaata cgctggttac cgccctggtt
     4021 ggcatcgatt tgacgaacaa tgtagaaaga gtaggcacct ttcgcttttg ccgctttggt
     4081 gatggcatca ttgacttctg gctggctacg atagaagccc tggacggtga ccgtgtcaaa
     4141 cggttcaatc agaacagcct gatcttnnnn nnnnnntgat ctttcggcag ttcgacaaca
     4201 ccgttaatta cgcgattact tgtttcttct gctttttcag catcggcttt atagaggtca
     4261 gcgactacac gccagttacc gctgttacca aaatcagaag tgtcgacaac ataaaaagag
     4321 gcggcacctt ctttatctgc gcgacgagaa acggctttca ccgcttcgcc aatagcatta
     4381 aaacgaccgg taaccactac acggtcaaaa ggtttaaccg ctgccgcttg ctccggtgtc
     4441 agttctgttg ctgcgttaac ggagaatgcc gtagcagaaa gcagtgccga cgccaggagg
     4501 gtgttcttaa gcttcataaa aataatcctt cgccttgcgc aaaccaggta ctggtattgt
     4561 tattaacgag aaacgtggct gattattgca tttaaacggt gtaactgtct gcgtcatttt
     4621 tcatatcaca ttccttaagc caattttaat cctgctcaaa tgaccgtcta tgcttaaaaa
     4681 aacagccgta tcagcatcaa tactactgaa gcaactgaat tgtataagtt aatttaatgt
     4741 taagtagt
//
LOCUS       DAWDRC010000301         4744 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7750_length_4744_cov_2.270420, whole genome shotgun sequence.
ACCESSION   DAWDRC010000301 DAWDRC010000000
VERSION     DAWDRC010000301.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7750_length_4744_cov_2.270420"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    993..1002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1219 a         1174 c         1068 g         1273 t
ORIGIN      
        1 tgtgataata tgggattatt gcggtttact ttgaggaaga taaaatgcga gtttatagca
       61 ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat aaaaaattgt
      121 cgactggagg gagttaaatc ctttacctca atatattgcc tgtcaggttt agttcacaaa
      181 cagtgcttcg ctatcaacga tgattccatc ttccgcacct ttaagcgcca ggtttgcctc
      241 gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac gagtctgttt
      301 aagcggcatc aaatattggt tttccattac accaacaccg gccaggcgaa gctcttccat
      361 tgcccccttt ttatcccctt tcgccatttt ttcgttggca atttttatcg cggcttcttt
      421 ttcgggtgtc gctacataac tctcagaaat tccgacagat gcattaatga caatatattg
      481 gtcatcattg acgttggtct ttttacctgg cttagcgaac tttgcccatt cggtactatc
      541 atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg gatcgccatg
      601 aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt gttcagagat
      661 acgctctgct gctgccgttt tctgggtttg cttaacttgc tgagtttgct gttgtgccgc
      721 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
      781 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
      841 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggacattttg aaagggttat
      901 cttacaactg tagttattat cgcaaagccg cttttagtct tacagttgta ggaatctgct
      961 tcctgtcgca gagaactatt cttatatacc acnnnnnnnn nntaatttat tatgtcgatg
     1021 taaatactga ttaattaaat ataaatacag ccataacatc gtaggttttc agtgaaccat
     1081 tttcaggata ttttctatga aagtaaactt aatgcttttt agcttatttt tatttgtctc
     1141 tattatggca tgcaatgttt ttgcattttc tatttctggt ggtggtagtg aggcgagcta
     1201 taaagagaca gaaaaaacgt cagtgatgac gacaacgcat tctacaaaac ttcagccatc
     1261 acaggcgatt ttgtttaaga tgagagaaga tgcaccacca ttaaacctca cagaagacat
     1321 cacgcctact tatccgacaa aggcgaatta tctcattcat cctttgcgat agcgtctgtc
     1381 acctggcagg tgaacaatca tgtctgatgc tgatgtgaac acctgcccaa atgagccatc
     1441 ctgacccact atcgcctgcc ataccccacc ataaagcgaa tcatcaagcc acaaaccggg
     1501 gcaagaaaca taaatatccc cgtcaccagt tcattaccgc cagttaacca catggtgaag
     1561 taataaatga gtgcagcgag gatcaccatg ccgaagaaag aagagatcgc cgataccatg
     1621 ttcagtacct gaccgatgta tccgcttacg cagacaatac acagggcgag aaacatccat
     1681 aaataaatca tagctcaacc tcctgccgcg ctaaaaaacc tgtaacttca gtcactttaa
     1741 tagtaagaca atccttaacc tctggcaatt tttgctaagc atcaatcaaa acagcatgtt
     1801 aaatgcaaga ctgttacgta cggaaaaata tttactttgc acgattaata atcagtagct
     1861 gaaagcagtc agcgagtgat aaagtacaga taccgcattc atgctcctcg tggttatgtc
     1921 ctgactagtc tttacactct ttacaggaac cattgtcgta catgatggcc taatcaattg
     1981 agtgtaccgc tgtcgacatg gaatcttctg cgatacaaca attcgtatct acagaaggta
     2041 actatgtttc caaaatgcaa attttcccgc gagtttctgc atcctcgcta ctggctcacg
     2101 tggtttgggc ttggtgtact ctggctttgg gtacagcttc cttatcctgt tctctgcttt
     2161 ctcggcacgc gtatcggcac aatggcgcga ccattcctga aacgtcgtga atctatcgcc
     2221 cgtaaaaacc tggaactttg tttcccgcag cattctgcga aagagcgcga gaagatgatt
     2281 gccgaaaact ttcgttcact cggcatggcg ctggtagaaa ccggcatggc gtggttctgg
     2341 cccgacagtc gcgtacgtaa atggtttgat gtggaagggc tggataacct taaacgcgca
     2401 caaatgcaaa atcgcggcgt aatggttgtc ggcgtccatt ttatgtcgct ggaactgggc
     2461 ggtcgggtga tgggactttg ccaaccaatg atggccacct atcgcccgca taataatcag
     2521 ctgatggaat gggtccagac ccgtggtcgc atgcgctcta acaaagcgat gatcggcaga
     2581 aataatctgc gcggcattgt cagtgcactg aagaaaggtg aagcggtatg gtttgctccc
     2641 gatcaggatt atggtcgtaa aggcagctcc ttcgcgccgt tctttgcggt ggaaaatgtc
     2701 gccacaacca atggcaccta tgtcctctct cgtctctctg gcgcagccat gttgaccgta
     2761 acgatggtaa gaaaagcgga ttacagcgga tatcgtttgt acatcacccc agagatggaa
     2821 ggctacccga cagatgaaaa tcaagccgct gcctatatga acaagattat cgagaaagag
     2881 atcatgcgcg caccggagca gtacctctgg atccaccgtc gctttaaaac gcgcccggtg
     2941 ggtgaatcgt cgttatacat ttaacaaact gctctcactc tttccctcct caaatcagcc
     3001 ataataacct catggttttt atggcttttt tcttaagttt ttttccttcg ctgaatatct
     3061 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacccgtaag ttactaaaag
     3121 taaataagaa aatgcctctt gtctcgtttc atttttaggc gtacattagc gccgtctcga
     3181 gcagtttagc tcagaattct ggggtgatgc ccacggtaaa atacaggacg tttaaggtcg
     3241 ttttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
     3301 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
     3361 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
     3421 catctggctc ctgtttgcat ttctgacgat ggtgtttatt ccgcgttttc gtgaatatgt
     3481 ttgctgctgg caggtagtaa accgtcggca cggaacatcg ctttaatccc acgaatcgcc
     3541 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgca catgcgtatc gccgtagtcg
     3601 ccaaagccaa tccctggaga gacacagacc ttcgcttcgt taagcaattt cttggcaaat
     3661 tccagcgagc ccatggcagc atacggttcc gggattttcg cccagacata catcgaagcc
     3721 ttcggcattt cgaccatcca gcccgcttca tgcagccctt tcaccagctc atcacggcgg
     3781 cgtttgtact gttcggcaat atcgcgcacg cactgttgat cgccctccag cgccgcaata
     3841 gccgccacct gcaacggcgt aaaggtgccg taatcgtgat agcttttaat acgtgccaac
     3901 gcgctgacca gcgttttgtt accgaccata aagccgatac gccagcccgc catgttgtag
     3961 cttttcgaca gcgtaaagaa ctcgaccgcc atgtcgcgtg cacctggcac ctgcatgatt
     4021 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcgtg gaccaccagc
     4081 acatcgtagc gtttcgccag cgccacaacc ttctcaaaga attccagttc cacgcattgc
     4141 gcggttgggt ttgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
     4201 atggcgcgct ccagttcatt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
     4261 tgcgccccgg caatcaccgc gccgtaaata tgaatggggt agcttggatt cggcaccagc
     4321 actgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
     4381 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
     4441 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
     4501 gggcgctggg ccaccgtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
     4561 ttacccatac tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
     4621 gcggtaatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
     4681 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
     4741 gcca
//
LOCUS       DAWDRC010000302         4733 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7767_length_4733_cov_2.184267, whole genome shotgun sequence.
ACCESSION   DAWDRC010000302 DAWDRC010000000
VERSION     DAWDRC010000302.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7767_length_4733_cov_2.184267"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1356..1365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1724..1733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2270..2279
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3639..3648
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4189..4198
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1072 a         1275 c         1133 g         1203 t
ORIGIN      
        1 ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg gtttaaaaac
       61 caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg ataacctgcc
      121 catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg tttgccttaa
      181 tgacgagaac gtcattagat ccggtttcca tcatatctac gactttaccg agatcgtagc
      241 cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtcc ccttcttcaa
      301 gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg ttcgccgcat
      361 cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc ttccagcttt
      421 ccagttggac ttgctgccac tgacccgcct tctggataaa ccagggctga tagtcaaaaa
      481 tgccttcggt ggaagaaaac actctgagcc acccacgaat accgtaagac gaacccattt
      541 ttcccaaaac gatgggatca acgggtgctt gcgcggtgag ttgtttgctc atcatgacca
      601 ccgtgacaga ttaagctgct ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa
      661 tagttgcgcc ctggccaacc cagtgagcga tgcgatccag atccaggcga gtgccttctt
      721 ctttttcgct agcgattggg ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg
      781 cattacggct gtcagcgaca acaacctggt agaacggacg ctttttagcg ccgtgacgtg
      841 ctaaacgaat agttaccata acatcctctt gtgtgaataa aacaaccgga ccccatcgag
      901 gaacggagtc cggtgtcata ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc
      961 aatcagcagt taataactct gtcgcataag gtcatttagc gaccaggaaa tcccggcggc
     1021 atcatccctt tcatgcttct catcatcttc gccatcccgc ccttcttcat tttcttcatc
     1081 atgcgctgca tgtcgtcgaa ctgcttcaga agacggttaa cgtcctgcac ctgcataccg
     1141 caaccggcag cgatacgacg cttacgcgaa cctttgatga tttctggctt agcgcgctct
     1201 ttcatcgtca tcgagttgat gatggcttcc atacgcacca gcactttatc gtccatctgc
     1261 gacttgacgt tatccgggat ctgccccatg cccggcaact tgcccatcag gctagccatg
     1321 ccgcccatgt ttttcatctg gcgcaactgt tcaagnnnnn nnnnncgatc agcgacagta
     1381 cgtcgcccat gccgagaata cgggaagcga tacggtccgg atggaacggc tccagcgcct
     1441 cggttttctc gccaacaccg aggaacttga tcggtttgcc agtgatgtga cgaatagaga
     1501 gcgccgcacc gccacgagca tcgccgtcga ctttagtcag taccacaccg gtcagcggta
     1561 acgcttcatt gaatgctttt gccgtattgg ccgcatcctg accggtcatg gcgtcaacca
     1621 caaacagggt ttcaactgga ttaatcgacg catggacttg tttgatctcg tccatcatcg
     1681 cttcgtcaac gtgcagacga ccggcggtat ccaccagcag tacnnnnnnn nnnacgtcgt
     1741 agaatttcag tttggcttct ttcagcgccg cgttaacgat atctaccggc ttctgaccaa
     1801 catcagaagg gaagaaatca acgcccacct gctctgccag cgtctcaagc tgtttgattg
     1861 ccgccgggcg ataaacgtcg gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca
     1921 ggaacttacc gagtttaccc acgctggttg ttttaccggc accttgcagg cccgccatga
     1981 gcacgaccgc aggcggttgc gcagccaggt tcagggtctg gttctcttcg cccatcgccg
     2041 caaccagttc gttacggacg attttgacga actcctgccc cggcgtcagg ctcttattaa
     2101 cttcatgacc aaccgctttc tcttttacgc gattgataaa ctcacgcact accggcagag
     2161 cgacgtccgc ctccagcagc gccatgcgca cttcgcgcag cgtatctttt acgttgtctt
     2221 cagtgaggcg tccacggcca ctcactgaag acaacgtaaa agatacgctn nnnnnnnnnc
     2281 cgcaatcgcg acacatcatc agtattttgc cgcagtataa catgaaggcg tctttgttgt
     2341 tatgcaacgg ttggagcagc gttcacctga cgctatactg cttctctttc ttattgctca
     2401 aactgtcgac attactatgc ccgtttttgc tctgctcgcg cttgtcgcct actccgtcag
     2461 tcttgcgctg attgttcccg gtctgctgca aaaaaacggc ggctggcggc gcatggctat
     2521 tatttctgcg gtcatcgcgc tggtctgcca cgcaatcgct ctggaagccc gcatcctgcc
     2581 cgacggtgat agcggacaaa acctcagcct gctgaacgtt ggttcattgg tcagtttgat
     2641 gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt ggctggttgt tgctacccat
     2701 tgtctatgcc tttgcgctta tcaacctggc gctggcgacc ttcatgccca atgaatacat
     2761 cacccatctg gaagcaacgc cggggatgct ggtgcacatt ggcttatcgc ttttttccta
     2821 tgccacgcta attatcgctg ccctgtacgc gctgcaactg gcgtggattg attaccaact
     2881 gaagaacaag aagctggcgt ttaaccagga aatgcctcca ttgatgagta tcgagcgtaa
     2941 aatgttccat atcacgcaga ttggcgtggt gctgctaacg ctcactcttt gcactggcct
     3001 gttctacatg cacaacttat ttagcatgga aaatatcgac aaggccgtgc tctctatcgt
     3061 ggcgtggttt gtctatattg tgctgctgtg gggacattat catgaaggat ggcgtggacg
     3121 ccgcgtcgtc tggtttaacg ttgcgggtgc agtcattctg acactggcct acttcggcag
     3181 ccgaattgtc cagcagttaa tcagctaacc ccagaaaagg agtttcccct ggaacacatt
     3241 tctactacta cgttgatcat tattctgatc atcatggtgg tcatttcagc ctatttttcc
     3301 gggtccgaaa ccggaatgat gactctcaac cgctatcgtc ttcgacatat ggcgaaacag
     3361 ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta agccagaccg cctgataagc
     3421 ctggtgttaa tcggcaataa cctggtcaat attcttgcct ccgcgctcgg cactattgtt
     3481 gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcaa ctggtgtgct gacttttgtc
     3541 gtactggttt ttgctgaggt attgccgaaa accattgccg cgctgtaccc ggaaaaagtc
     3601 gcttatccga gtagttttct actggcgccg ctacaagtnn nnnnnnnncc tgatgatgcc
     3661 gctagtctgg ttactaaatg ccatcacccg gattttgatg cgtatgatgg gtattaaaac
     3721 cgatatcgta gtcagcgggt ctttgagtaa agaagagttg cgcaccatcg tgcacgaatc
     3781 gcggtcacaa atttcacgtc gcaatcagga tatgctgctg tcggtgctcg atctggaaaa
     3841 gatgaccgtc gatgacatca tggtgccgcg cagtgaaatt atcggtattg atatcaacga
     3901 tgactggaaa tcgattctgc gccaactctc ccactcacct cacgggcgca tcgtgctcta
     3961 ccgtgattcg ctggacgacg ccatcagtat gctgcgtgtg cgtgaagcct ggcgactcat
     4021 gtcggagaaa aaagagttca ccaaagaaac catgctacgc gccgcggacg agatctattt
     4081 tgtcccggag ggtacgccgc tcagcacaca gttggtgaag tttcagcgca acaaaaagaa
     4141 agtcggactg gtcgtcaacg agtatggaga cattcaggga ctggtgacnn nnnnnnnntt
     4201 aactggcatc taccggaaga tgatgctcgt actgttaacg gcgtcattct ggaagcgctg
     4261 gaggagatcc cggtagcagg cacccgcgtg cgtataggcg agtacgatat cgatattctc
     4321 gacgtacagg acaatatgat taagcaggta aaagtttttc ctgtaaaacc gctgcgcgag
     4381 agtgtggcgg agtaacgaaa acggcccggc attcgcatgc tgggccgtga tttattacga
     4441 aagcagaaat taagcttttg ctttcgctac agtcaccatc gccgcacgaa tcgtacgacc
     4501 gttcagcgta taacctttct gcataatgcc cagtacgtta cctggcgcaa cgtcatcaga
     4561 ttccaccatt gcgatggcct gatgcacatt tgggtccagt gggacgttgg tttcggcgat
     4621 cacttcaacg ccaaacttac gcacaacatc cagcatcgac ttcagcgtca gctcaatacc
     4681 ttcaaccatc gcggacatat ccgggttagc tttatcagcc acttccagcg cac
//
LOCUS       DAWDRC010000303         4701 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7822_length_4701_cov_1.973095, whole genome shotgun sequence.
ACCESSION   DAWDRC010000303 DAWDRC010000000
VERSION     DAWDRC010000303.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7822_length_4701_cov_1.973095"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1305..1314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1214 a         1194 c         1164 g         1119 t
ORIGIN      
        1 aatgagcttt tcccgcgaga tcaatctggg cctggcgcaa catgagttcg aattgttctg
       61 tcagcctttg cttaatgcgc gcagccagca atgtattggt gtagagattt tgctgcgctg
      121 gaacaatcca cgtcagggct ggatttcacc ggatgtgttt attcctatcg cggaagaaca
      181 tcatttaatt gtgccactaa cccgctatgt gatggccgaa accattcgtc agcgccatgt
      241 tttcccaatg agtagtcagt ttcatgttgg cattaacgtc gcacccagcc attttcgccg
      301 tggtgtgctg ataaaagatc tcaatcagta ctggtttagc gctcacccga ttcagcaact
      361 gatcctcgaa atcaccgaac gcgatgcctt actggatgtt gattaccgga ttgcccgcga
      421 gctgcatcgt aaaaacgtca aactggcgat tgatgacttc ggcaccggca acagttcatt
      481 ttcctggctt gaaacattac gtcctgacgt tctgaaaatt gataagtcat ttaccgcagc
      541 tataggttct gatgcggtta actcgacggt gaccgatatc atcatcgcac tggggcaaag
      601 actgaatatt gaactggtgg cggagggcgt agaaacgcaa gaacaggcga agtatttgcg
      661 cagtcatggc gtgcatattt tgcaagggta tttgtacgca cagccgatgc cgctacgtga
      721 ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc cggcataatg gacatatcac
      781 acccattatg ccgttacgtt aacgcttact catcttcatc gtgcgccggt tgctctttaa
      841 caatgcgaac cagatcaaca cgataatcat tggcttcaat gatggtgata tgcagtggcc
      901 ctacatcaat cacatcgccc acacggggaa tgtgaccatt tgccgagatc acgaggcccg
      961 cgaccgtcgc gatatcatcg tcatcggcaa ggtgctcaac atcaagcgcc tgctgcaagg
     1021 catgcaaatc tgtaccgcct tttaccagcc agccgtcacc gtcggtaatg atttccggcg
     1081 tttcgtcagc gtccgggaat tcacccgcaa tggcttccag cacatccagc ggcgtgacca
     1141 gaccttgtac cacaccaaac tcgttggtca cgataacaaa gctcccgcga gcacgacgca
     1201 gcacgcccag caggttgatc ggatcgaggg tttccgggac gataatcgcc ggagacgccg
     1261 aagcaatcgc cgccacatca acgccctctt ccagcgccac cagcnnnnnn nnnncggcaa
     1321 acgcaccttc cgggatcggc atcggcatcg ggttatcggc atcgtgctga acattggcct
     1381 gacgtttccc gcccatcaaa cgcaggatgg catcggcagt acgcgctcgc agcggcaaag
     1441 tcgactggtg acgaataaag ttgcgacgcg caatctggtt aaacacttcg atgatgatcg
     1501 agaagccaat cgcggcatac aggtaacctt tcggaatgtg gaaaccgaaa ccttctgcca
     1561 ccagactcag accaatcatt aacaggaagc tcagacagag caccaccacc gtggggtgct
     1621 ggttaacgaa tcgcgtcagc ggttttgatg ccagcagcat aactgccatc gcaatcacta
     1681 ccgccgccat catcaccggc aaatggttaa ccatccctac tgcagtaatt accgcatcca
     1741 acgagaagac ggcgtcaagg atgacaatct gtgtgacgac cacccagaaa ctggcgtagc
     1801 ctttaccgtg gccggaatca tgatcgcggt tttccagccg ttcatgcagt tcggttgttg
     1861 ctttgaacag caagaatatg cccccgaaca acataatcag gtcgcgtccg gagaaggaga
     1921 aatccatgac ggtaaatagc ggtttggtca gcgtgaccat ccatgaaatc agcgacagca
     1981 gccccagacg cataatcagc gccagtgata atcccagcaa acgcgcttta tcgcgttgtt
     2041 ttggcggcag tttgtcagca agaatggcga tgaagaccag gttatcgata cccagcacaa
     2101 tttcgagaac aacaagcgtg agtagccccg cccaaatcga ggggtccatt aagaattcca
     2161 tgacaagctc ctgcttaagg aatagctatt cgacgccaga aataatgcag gcgtaacgac
     2221 aaaatgcaaa cgaaaggtgc ggcataaagt gccagaaagg caggcgttaa aaggcctgat
     2281 gctgaaatga cgtcggtgac gatccatact gcgggctact gccctatact ccatggttgt
     2341 taaacaggag ttaaacatat cagagacgcc tctgatttgg caaagattta ccttcctttg
     2401 caaacgaatg tgacaaggat attttacctt tcgaaatttc tgctaatcga aagttaaatt
     2461 acggatcttc atcacataaa ataatttttt cgatatctaa aataaatcgc gaaacgcagg
     2521 gggtttttgg ttgtagccct tatctgaatc gattcgattg tggacgacga ttcaaaaaca
     2581 catctggcac gttgaggtgt taacgataat aaaggaggta gcaagtgacc attgctattg
     2641 ttataggcac acatggttgg gctgcagagc agttgcttaa aacggcagaa atgctgttag
     2701 gcgagcagga aaacgtcggc tggatcgatt tcgttccagg tgaaaatgcc gaaacgctga
     2761 ttgaaaagta caacgctcag ttggcaaaac tcgacaccac taaaggcgtg ctgtttctcg
     2821 ttgatacatg gggaggcagc ccattcaatg ctgccagccg cattgtcgtc gacaaagagc
     2881 attatgaagt cattgcaggc gttaacattc caatgctcgt ggaaacgtta atggcccgag
     2941 atgatgaccc tagctttgat gaactggtgg cactggcagt agaaacaggc cgtgaaggcg
     3001 tgaaagcact gaaagccaaa ccgattgaaa aagccgcgcc agcacccgct gccgcagcac
     3061 ccaaagtggc tccaactccg gcaaaaccaa tgggaccgaa cgactacatg gttattggcc
     3121 ttgcgcgtat cgacgaccgt ctgattcacg gtcaggtcgc cacccgctgg accaaagaaa
     3181 ccaatgtctc ccgtattatt gttgttagtg atgaagtggc tgcggatacc gttcgtaaga
     3241 cactgctcac ccaggttgca cctccgggcg taacagcaca cgtagttgat gttgccaaaa
     3301 tgattcgcgt ctacaacaac ccgaaatatg ctggcgaacg cgtaatgctg ttatttacca
     3361 acccaacaga tgtagagcgt ctcgttgaag gcggcgtgaa aatcacctct gttaacgtcg
     3421 gtggtatggc attccgtcag ggtaaaaccc aggtgaataa cgcggtttcg gttgatgaaa
     3481 aagatatcga ggcgttcaag aaactgaatg cgcgcggtat tgagctggaa gtccgtaagg
     3541 tttccaccga tccgaaactg aaaatgatgg atctgatcag caaaatcgat aagtaacgta
     3601 ttgtgttgat tatcactcag ttttcacact taagtcttac gtaaacagga gaagtacaat
     3661 ggagattacc actcttcaaa ttgtgctggt atttatcgta gcctgtatcg caggtatggg
     3721 atcaatcctc gatgaatttc agtttcaccg tccgctaatc gcgtgtaccc tggtgggtat
     3781 cgttcttggg gatatgaaaa ccggtattat tatcggtggt acgctggaaa tgatcgcgct
     3841 gggctggatg aacatcggtg ctgccgttgc gcctgacgcc gccctggctt ctatcatttc
     3901 taccattctg gttatcgcag gtcatcagag cattggtgca ggtatcgcac tggcaatccc
     3961 tctggccgct gcaggccagg tactgaccat catcgttcgt actattaccg ttgctttcca
     4021 gcacgctgcg gataaggcag ctgataacgg caacctgaca gcaatttcct ggatccacgt
     4081 ttcttctctg ttcctgcaag caatgcgtgt ggctattccg gccgtcatcg ttgcgctgtc
     4141 tgttggtacc agcgaagtac agaacatgct gaatgcgatt ccggaagtgg tgaccaatgg
     4201 tctgaatatc gccggtggca tgatcgtggt ggttggttat gcgatggtta tcaacatgat
     4261 gcgtgctggc tacctgatgc cgttcttcta cctcggcttc gtaaccgcag cattcaccaa
     4321 ctttaacctg gttgctctgg gtgtgattgg tactgttatg gcagtgctct acatccaact
     4381 tagcccgaaa tacaaccgcg tagccggtgc gcctgctcag gctgctggta acaacgatct
     4441 cgataacgaa ctggactaac aggtgagcga aatggttgat acaactcaaa ctaccaccga
     4501 gaaaaaactc actcaaagtg atattcgtgg cgtcttcctg cgttctaacc tctttcaggg
     4561 ttcatggaac ttcgaacgta tgcaggcact gggtttctgc ttctctatgg taccggcaat
     4621 tcgtcgcctc taccctgaga acaacgaagc tcgtaaacaa gctattcgcc gtcaactgga
     4681 gttctttaac accccgccat t
//
LOCUS       DAWDRC010000304         4698 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7825_length_4698_cov_2.916433, whole genome shotgun sequence.
ACCESSION   DAWDRC010000304 DAWDRC010000000
VERSION     DAWDRC010000304.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7825_length_4698_cov_2.916433"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1304 a         1052 c         1022 g         1320 t
ORIGIN      
        1 ttcactcact atactttgtt tcataaggat ttcaggatct gccagactgc gcagaaatga
       61 tgcttacgaa tacacagtaa aggcaatgtc atttccgata cagagcctga cattgccata
      121 atgagctatt tatctgaaaa acgacagaat atgatgtttt atcgtaacgt aattttaagt
      181 tctcaactta ttgagacata ttgtcttttt tacccatgtg gtcatttttc atcccatccg
      241 ttttgctcat gtgttctttc tccattttct ctttatccat tgcatttttg cacataccat
      301 ccttgcacat tttatcatgc gcgctggaca tgctgccttt tacttcatgt gttttatcca
      361 ttgtgtctgc tgcctgagca ttgaacatga acagcgcgga tagtacagtt gcagaaataa
      421 tatttttcat ggttcttcct catttttaac aattgtatca acaaccacca aaccagttat
      481 aaccctggtc ttcccagtac ccccccggaa aatgattagt gacctctata acctgaacat
      541 gcttggggtt tttatatccc agcttagtag ggatacgtat ctttatggga tagccatatt
      601 cttttggcaa taccctgtta ttccatgtca atgtcagcaa tgtttgtgaa tgtagtgctg
      661 tcgccatatc aatactggtg tagtaaccat cgacgcaacg aaaactgacg tattttgccc
      721 gcatatcggc accaatcagc gtcaggaaat gccggaatgg tatccctccc cattttccta
      781 ttgcactcca tccttcaaca cagatatgac gggttatctg actcacatgc tgcatgttat
      841 acaattcaga caaaaaccag ttacggttat cacgtaccag cccccgtatt tccaatttat
      901 aatcctggcc atcaattact gggatctctt cttctccata gaaggcatta aaagggaatg
      961 gagtggtaat gtcctctgga agatattctg gtgccacact gtttttgctg aacagaaaac
     1021 tttgaatccg gtcattaaat ctggatatac ggaacaatgc tttttcaata tcatcattat
     1081 tgcttatatc acagccagtc agcatcataa ttcccccaag cgtcagtccc tgttggagta
     1141 aacgacgtct gtccggcgca aggatttttt ctgcatcttt caccacgtaa tgggcatcac
     1201 tgtcagacaa aaaacgtttt ttcttcatta gtgaccccgt atcatagata acaatgcacg
     1261 cggaaccaat aacaccataa ccaggtgaat aataatgaac agtaccataa tgttcatgca
     1321 cagaaagtgg atataacgcg ctgtatcata accaccgaat agtatagtca gaagggaaaa
     1381 ctgaacgggt ttccataaaa ccagaccaga caatagaaga gcagcgccat ctaaaataat
     1441 cagaatatag gcgacttttt gcaccatatt gtattcctgc atattcgtat gatgcagctt
     1501 tccatacagt gcctgcgtaa gggatttttt cagtgaggtc catgacagcg ggaaaaactt
     1561 gctccggaaa cgtccgctac aaattcccag agtaagatag atcgtggcat taatcagcag
     1621 aatccacatc agggcgaagt gccacagtaa cgcaccgcca agccagccac cgagagttaa
     1681 tgctgccgga tagttaaaag aaaacaaagg agaagcatta taaatgcgcc atccactaca
     1741 tatcatgcct gcgacagtaa cagcattaat ccagtggcaa cagcgtaacc acagaggatg
     1801 tatttgtttt aacggtaatg gctgcattat gtgatctctg tctgtaaact aagtatatta
     1861 tggaaaggaa tgttcatcac atcctcacaa gagtttaaaa aaatgtgaca attcatcgtc
     1921 aaatgctggg gtaaaattca gataaagaat atgtggataa cttttgatga ataacgtaaa
     1981 aaaaatactg ctgatggaag atgattatga tattgcagct ctgttgcggc ttaatctgca
     2041 ggatgaaggg tatcagatag ttcatgaagc ggatggcgcc agagctcgtt tattactaga
     2101 caagcagacc tgggatgccg taatacttga tcttatgctg cctaatgtta atgggctgga
     2161 gatttgccgt tatatccgtc agatgacccg ttatctgcct gtgattatca tcagtgcccg
     2221 taccagcgaa acccaccgcg tcctgggact ggaaatgggg gctgatgact atctaccgaa
     2281 acccttttcc attcctgagc tgattgcccg catcaaagcg ttgtttcgtc gtcaggaagc
     2341 catggggcaa aatattctcc tggcaggtgg actgatttgc tgtcacggtc tgtgcatcaa
     2401 tccattttca cgtgaagttc atttgcataa taaacaggtt gatcttaccc cacgcgagtt
     2461 tgatctgctg ctctggtttg cacgtcatcc tggcgaagtt ttttcccgtc tttcactgct
     2521 ggataatgtc tgggggtatc agcatgaagg atatgagcat acagtcaaca cgcatatcaa
     2581 ccgtcttcgt gccaaaattg aacaggatgc agcagagcca aagatgatcc agaccgtctg
     2641 gggaaaaggg tataggtttt cagttgacaa tgcaggaatg cgataaatga attgtagcct
     2701 gacattaagc cagaggttaa gcctagtatt tacagtcgtt ttgctgtttt gcgcagcagt
     2761 gacatgtggc gttcatattt acagcagtaa tctgtatggc aatgcaatgg tacagcgttt
     2821 atctgcaggg ctggcgcaac agattgtcat cacggagtct ctgctggata atcgtgggca
     2881 ggtgaatcac cggacattaa agagtctgtt tgagcgtctg atgacgctta atcccagtgt
     2941 ggagctgtat attgtctcgc cggaaggtcg gctgcttgtg gaggccgccc ctccaggtca
     3001 tatcaaacgt cggtatatca atatagcgcc cttgaaaaaa tttctctccg gtgctgtctg
     3061 gcccgtatat ggtgatgaac cccgaagtgt aaataagaca aaagttttca gtaccgcacc
     3121 gctttacctg agggatgttc tgaaaggata tctgtatatt attttacagg gagaggaact
     3181 taatgctctt actgatgcag cctggacaaa ggcactatgg aatgcactgt actggtcgct
     3241 gtttctggta gtgatatgtg gtctgctgtc gggtatgctg gtctggtact gggtaacccg
     3301 tcccatacag caactaactg aaaatgtcag cgggatagag caggacagta ttagtgccat
     3361 taaacaactg gcaattcagc gccctgccac cccccctagc aacgaggtcg agatattaca
     3421 caatgccttc attgaactgg cccgtaaaat atcctgtcag tgggatcaac tttcagaaag
     3481 tgatcaacag cgccgtgaat ttattgccaa tatctcccat gatttacgga cgccattaac
     3541 atcacttctg ggatatctgg aaaccctgtc aatgaagtcg gattcgctat catcagagga
     3601 ctgtcataaa tatctgacaa cagctctccg gcagggacac aaggtgaggc atctgtcctg
     3661 tcagcttttt gagctggcac gtcttgagca tggtgctata aaacctcaac tggagcaatt
     3721 ttctgtctgt gaacttattc aggatgtagc tcaaaaattt gagctcagca tagaaacccg
     3781 tcgattgcaa ctaagaatta tgatgtcaca ttccctgcct cttatcaggg cagatatttc
     3841 aatgatagag cgtgtgataa caaatttact ggataatgct gtacgccaca cacctccgga
     3901 aggctcgatc aggctgaaag tctggcagga agataatcgg ttgcacgtcg aagtggctga
     3961 cagcggccct ggactaactg aagatatgcg aactcatctt ttccggcggg catcagtgtt
     4021 atgtcatgaa ccgtcagaag agccccgggg aggactggga ttgctgattg tacgcaggat
     4081 gctggtacta cacggtggtg atatcaggtt gactgattca acgactggag cctgctttcg
     4141 tttttttctt ccattataac atcaggcggc atattttggg gtggttatgt gtatctgcct
     4201 ttgtaaaagg gatacaagtt ctgtagtgga gcacaaaatc aggacaccgg aataacctgt
     4261 ttccactttt cttcatgtaa gcaaggcggt aaaccatcgt tgttcgtgtg aggtcgataa
     4321 acgttgtaat aaccattaat ccactggttt atatcacgta ccgcatggat aaaatcacca
     4381 taaccacctt tcggaagcca ttcattttta aggctgcgaa agactctttc catcggcgaa
     4441 ttatccaggc cattccctct gcaactcata ctttgcatta ccccataacg ccagagtaac
     4501 tttctgtatt tattgctttt atactgaaca ccttgatctg aatgaaacag caggcggcca
     4561 tcacgcggtc gagtttccag tccgttacgc aaagccctac acaccaactc agcatcagcg
     4621 gttaatgaga gggctgaacc gataatccgc cgtgaatata aatcaacaac gagcgcgagc
     4681 taacaccatt tgtcctgc
//
LOCUS       DAWDRC010000305         4695 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7835_length_4695_cov_2.422845, whole genome shotgun sequence.
ACCESSION   DAWDRC010000305 DAWDRC010000000
VERSION     DAWDRC010000305.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7835_length_4695_cov_2.422845"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3190..3199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1078 a         1198 c         1281 g         1128 t
ORIGIN      
        1 ttattaatat ttgtaattaa ctgaatatca acgcttattt aaatcagact aaagactttc
       61 tctctctgtc ataaaactgt cataaacctt acatataact gtcacctgtt tgtcctattt
      121 tgcttctcgt agccaacaaa caatgcttta tgaatcctcc caggagacat tatgaaagtt
      181 atgcgtacca ccgtcgcaac tgttgtcgcc gcgaccttat cgatgagcgc tttctctgtg
      241 tttgcagaag caagcctgac aggtgcaggt gcaaccttcc ctgcgccggt gtatgccaaa
      301 tgggctgaca cttaccagaa agaaaccggt aataaagtta actaccaggg tatcggttct
      361 tctggtggtg taaaacagat catcgccaat accgttgatt ttggtgcttc tgacgcgccg
      421 ctgtctgacg aaaaactggc tcaggaaggc ctgttccagt tcccgaccgt gattggcggc
      481 gtggtgctgg cggttaatat tccaggtctg aagtctggcg aactggtgct ggatggtaaa
      541 accctcggcg acatctacct gggcaaaatc aagaagtggg atgatgaagc catcgccaaa
      601 ctgaatccgg gtctgaaact gccttcacag aacattgctg tagtacgccg cgcagatggc
      661 tccgggactt ccttcgtctt caccagctac ctggcgaaag tgaacgaaga gtggaaaaac
      721 aacgttggta ctggctctac cgtaaaatgg ccgatcggtc tgggcggtaa aggtaacgac
      781 ggtatcgccg cgttcgttca gcgtctgccg ggtgcaattg gttacgttga atatgcttac
      841 gcgaagcaga acaacctggc gtacaccaaa ctgatctccg ctgatggcaa accggtgagt
      901 ccgaccgaag aaaacttcgc taatgcagca aaaggcgcag actggagcaa aacattcgct
      961 caggatctga ccaaccagaa aggcgaagat gcatggccta ttacctctac cacgttcatt
     1021 ctgatccata aagatcagaa gaaaccagaa caaggcaccg aagtgttgaa gttcttcgac
     1081 tgggcataca aaaccggggc taaacaggcg aacgacctgg attacgccag cctgccggat
     1141 agcgtagttg aacaggttcg cgctgcgtgg aagaccaata ttaaagacag tagcggcaag
     1201 ccgctgtact aataaaactc caggccgggt acggtgtttt acgccgcatc cggcattaca
     1261 aaatgacttt gtaaacgcgt ttaactgaag agtaacttat ggctgcaacc aagcctgctt
     1321 ttaacccacc gggtaaaaag ggcgacataa ttttcagcgt gctggtaaaa ctggcggcgc
     1381 tgattgtgct attgatgttg ggtggcatta ttgtctctct gatcatctca tcctggccga
     1441 gcattcagaa atttggtctg gctttcctgt ggaccaaaga gtgggatgcg ccgaacgata
     1501 tctacggggc gctggtgccg atctacggta cgctggtgac ctcgtttatc gcgctgctga
     1561 tcgccgtccc ggtgagtttt ggtatcgccc tgttcctgac cgagcttgcg cctggctggc
     1621 tgaaacgccc gctgggtatc gccattgagc tgctggctgc cattccaagt atcgtttacg
     1681 gcatgtgggg cctgtttatc tttgcgccgc tgttcgccgt ttacttccag gagccggtcg
     1741 gcaatatcat gtcgaatatc ccgattgtcg gcgcgctgtt ctccggcccg gcctttggta
     1801 tcggtatcct cgcggcaggc gtgatcctcg ccatcatgat tattccgtac attgcggcgg
     1861 taatgcgcga tgtattcgaa caaaccccgg tgatgatgaa agagtcggcc tatggtatcg
     1921 gctgcaccac ctgggaagtt atctggcgta tcgttcttcc gttcaccaaa aatggggtta
     1981 tcggcggcat catgctgggt ctggggcgcg cgctcggtga aaccatggcg gtgaccttta
     2041 tcattggtaa cacctaccag ctcgacagcg cctcgctgta tatgccgggc aacagtatca
     2101 cctctgcgct ggccaacgaa tttgcggaag cggaatccgg tctgcacgtc gctgcactga
     2161 tggaactggg cctgatcctg tttgtgatta ccttcatcgt cctcgccgca tcgaagttta
     2221 tgattatgcg cctggctaag aatgaggggg cacgctaatg gctatggttg aaatgcaaac
     2281 cactgcggca ctggctgaat cccgccgcaa aatgcaggcg cgtcgccgcc tcaaaaaccg
     2341 tattgcgctg acgctctcaa tggcgacgat ggccttcggt ctgttctggc tgatctggat
     2401 tttaatgtcc accatcactc gcggtatcga tggtatgtcg ctggcgctgt tcaccgaaat
     2461 gacgccacca cccaatacgg aaggcggtgg tctggcgaac gccctggcgg gtagcggcct
     2521 gttaattttg tgggctaccg tattcggtac gccgctgggc attatggcgg ggatttacct
     2581 ggctgaatat ggtcgtaaat cctggctggc agaagtgatt cgcttcatta acgacattct
     2641 gctctctgcg ccgtcgattg tggttggtct gtttgtttac accattgtgg tggcgcagat
     2701 ggagcacttc tccggctggg cgggcgtgat tgcgctggcg ctgttgcagg tgccgattgt
     2761 tatccgcacc accgagaaca tgctgaaact ggtgccggac agcctgcgtg aagcggctta
     2821 tgcgctgggt acgccgaagt ggaagatgat cgccgcgatc acgctgaaag cgtcggtgtc
     2881 cgggatcatg accggtatcc tgctggcgat tgcccgtatt gctggtgaaa ccgcaccgct
     2941 gctgtttact gcgctctcca accagttctg gagcacggac atgatgcagc cgatcgccaa
     3001 cctgccggtg acgatcttta agtttgcgat gagcccgttt gcggaatggc agcaactggc
     3061 ctgggccggg gtgttgatca ttaccctgtg cgtactgctg ctgaacattc tggcgcgcgt
     3121 tgtttttgcg aagaataaac acggttaaca ctttgctgga tgcggcgtaa acgccttatc
     3181 cggcctacgn nnnnnnnnng caatgagtat ggttgaaact gccccgagta aaattcaggt
     3241 tcgtaatttg aacttctact acggcaaatt ccatgccctg aaaaacatca acctggatat
     3301 cgccaaaaac caggttacgg cgtttatcgg gccgtccggc tgcggtaaat caacgctgct
     3361 gcgtaccttc aacaaaatgt ttgaactgta cccggaacag cgtgcggaag gtgaaattct
     3421 gctcgatggc gacaacatcc tgaccaactc tcaggatatc gccctgctgc gtgcgaaagt
     3481 gggcatggtg ctccagaaac cgacgccgtt cccgatgtcc atttacgaca acatcgcttt
     3541 tggcgtgcgt ctgtttgaga agctctcccg tgccgacatg gacgagcgcg tgcagtgggc
     3601 attgaccaaa gccgcattgt ggaacgaaac caaagataaa ttgcaccaga gcggttactc
     3661 tctctctggt ggtcagcaac agcgtctgtg cattgcgcgt ggtatcgcca ttcgcccgga
     3721 agtgctgctg ctcgacgaac cgtgttcggc actcgaccct atctctaccg ggcgtattga
     3781 agagctgatc accgaactga agcaggatta caccgtggtg atcgtcaccc acaacatgca
     3841 gcaggctgcg cgttgttccg accacacggc gtttatgtac ctgggcgaat tgattgagtt
     3901 cagcaacacg gacgatctgt tcaccaagcc agcgaagaaa caaacagaag actacatcac
     3961 cggtcgttac ggttgattca ggagtgcgtt atggacagtc tcaatcttaa taaacatatt
     4021 tccggccagt tcaacgccga actggaaagc atccgcacgc aggtgatgac catgggcggc
     4081 atggtggagc agcagctttc tgatgcaatc accgcgatgc ataaccagga cagcgatctg
     4141 gcgaagcgcg tcatcgaagg cgacaagaac gtcaacatga tggaagtggc gatcgatgaa
     4201 gcctgcgtgc gcattatcgc caaacgtcag ccgacggcga gcgacctgcg tctggtgatg
     4261 gtaatcagta aaaccattgc cgagctggag cgtattggcg acgtggcgga caaaatctgc
     4321 cgtactgcac tggagaaatt ctctcagcag catcagccat tgctggtaag tctggagtcg
     4381 ctgggccgtc ataccatcca gatgctgcac gacgtgctgg acgcgttcgc gcggatggac
     4441 attgacgaag cggtacgtat ttatcgtgaa gataaaaaag tcgatcagga gtacgaaggt
     4501 attgttcgtc aactgatgac ctacatgatg gaagattcgc gtaccattcc gagcgtactt
     4561 accgcgttat tctgtgcgcg ttctatcgaa cgtattggcg accgctgcca gaatatttgt
     4621 gagtttatct tctactacgt gaaggggcag gatttccgtc acgtcggtgg tgacgagctc
     4681 gataaactgc tggca
//
LOCUS       DAWDRC010000306         4667 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7884_length_4667_cov_2.439939, whole genome shotgun sequence.
ACCESSION   DAWDRC010000306 DAWDRC010000000
VERSION     DAWDRC010000306.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7884_length_4667_cov_2.439939"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1218 a         1213 c         1058 g         1178 t
ORIGIN      
        1 ttatgtatca acaagttaca ttgataatta acgctaccat taataaaaat accccacaag
       61 gtccgaaaaa aaacgccggc aatgtgccga cgtctttgaa aagaaaggat tgtctatgtt
      121 atcgcccgtt attcattcag ccgggttgac ttatccgcgc caattgctcg aaatagtctg
      181 gaaaagtttt agcggtacat tttggatcaa gaatcgtcac aggcgtatct gataatgcga
      241 ccagcgagaa acacatcgcc atccggtgat cattataagt ggcgatctca gcaaatttca
      301 gtttttccgg tggcgtaatg cgaatgaaat catgcccctc ttcgacttcc gcaccgactt
      361 tacgcagttc tgttgccatc gcaaacaggc gatcggtctc tttaacacgc cagttataga
      421 tattgcgcag cgtggtggtg ccttttgcaa ataatgccgc ggtggcaatg gtcatcgccg
      481 catcgggaat atggttcata tccatatcaa tggcgttcag ttcaccacgc gtgcaggaaa
      541 tataatcatc gccccagcaa atggtcgcgc ccattttttc cagaacatca gcaaagcgaa
      601 tatcaccctg catactgttg cgtccaatgc cggtcacttt tacagtgccg cctctgattg
      661 ctgctgctgc caggaaataa gaagccgaag atgcatcgcc ttcaaccaaa taagtacccg
      721 gagactggta agactgcccc ccttttacga caaattgttg atagtgctga ttttcaattt
      781 caacaccaaa cgtcttcatc agattgagtg tgatgtcgat ataaggttta gaaaccagat
      841 cgcctttaat acgaatcacc gtatcttccg gcgcaagagg tgcagtcatt aacagtgcgg
      901 tgaggaactg gcttgaaacg gagccatcaa catcaacgtt gccgccagta aagccgccct
      961 gtaaacgcaa tggcggataa ttttcttgtt ccaggtaagt gatcttcgcc ccgccctggc
     1021 gcagcgcatc caccagatga ccaatcgggc gttctttcat tcgcggttca ccggtcagta
     1081 caatatcatt gctacccagg caaagtgctg ccgccagcgg acgcattgcc gttccggcgt
     1141 taccgaggaa caactcccgg gcactttctg cgtgtaatgg accaccgtta ccgataattt
     1201 cgcaacgcgt acgatcggct gaaagcgtat agcttacccc taacgctgtt aatgcattca
     1261 gcatatggtg cacgtcatcg ctatccagca gattggttaa tactgttttg ccgtgtgcta
     1321 atgccgccag caataaagcg cggttagaaa cgctcttgga accgggcaga ttaatagtgc
     1381 catcgacacg agcgatgggt tgtaacgtta gggattccat gaactcaact ctctacaaca
     1441 gaaataaaaa ccccacaggc tggctgtggg gattatgcaa aatttcgaca ttagccgtga
     1501 cgacgttcga actcaaccat gaagtctgtc agtgctttaa caccttccag cggcatggcg
     1561 ttataaatag acgcacgcat tccgccgacc actcggtgac ctttcagcgc atgtaagccc
     1621 gcagcgaaag actcttcgag gaacaattta tcaagcgcgc tatccgccaa ctggaacggc
     1681 acgttcatcc gcgaacggtt agctttcgcc acatcattgc ggtagaaatc gctgttatca
     1741 atcaccccat acagcagttc tgctttttgc tgattgatct tatccattgc aaccacaccg
     1801 ccgttcgctt tcagccattt gaagaccagg ccagataagt accaggcaaa tgtcggcggc
     1861 gtgttgaaca tggaatcgtt atcgttgagg atggagtaat ccagaatcga tgggcaggcg
     1921 atattcgctt tgcccagcaa atcttcacga acgatgacga ttgtcaggcc agccgggccg
     1981 atatttttct gcgcgccagc gtaaattaca ccataacggc tgacatcaat cggacgggaa
     2041 agaatggttg aagagaagtc agcggcgacc accacgtctt taccgaagtc tggtttttaa
     2101 tcgagagcga taccgtcaat ggtttcgttc gggcagtaat gcatataagc agcattatca
     2161 gagagttgcc attcactcat tggcttaact gcgcgaagac catcaacggt cactttcgca
     2221 tcaaacacat ttggtgtgca gtattttttc gcttctttga tagcacttgc tgcccagtaa
     2281 cccgcatcaa cataatctgc agtggtttta tcaccaagaa tattcagcgg tacagcagca
     2341 aattgaccgc gaccaccgcc atggcagaac aatactttgt agttggaggg gacgttaaga
     2401 agatcgcgaa aatccttctc ggcttcctct gcaacctgaa tgaactcttt gccacggtga
     2461 ctcacttcca tcaccgacgt accaagaccg ttccagtcgc gcagttcctg ttgagcctgt
     2521 ttaagcacct ctgccggtag cattgccgga ccagaactaa aattgaagat ttgagccatt
     2581 tcccctcacc acgttgcgtt gctattaacc cgcggtcaat cgattgcgac cacaggtaat
     2641 acattattcc agtggctatc ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac
     2701 cgttgagggc aaaaatgtgg cttgcatcac acaaaagaat ctctcggatt attagcaaaa
     2761 agccgatgtt tttgctgtca ggtcacccgt ttagctcgac ttgccttcgt ccattctgga
     2821 tttgcacagc gcggacataa ttgtttttcc cccgcgttca tatgaatgat ttcactgcaa
     2881 cggatgcagg catagtccgc cgactccggc accactttgt aacgctgagt acagtcactg
     2941 ggtaaaataa gtaaccccgg tttcaccttg tcatcttcaa agaaataaac actgatttgc
     3001 ccgttggttt cgagaatcgc cagacgtacc tgccccaact gctctacacc gcgcaatcgt
     3061 agctccataa agaactcaaa ttccgtcatg ttggagttat tgagtttcga ccaggccagc
     3121 tcgccatctt caataatgac aaccggcttg ccttccagaa gatcttccag tttttcactg
     3181 tgcgccatca accacattac caggcggtat aacaacgcca gagtaataaa gacgataagt
     3241 accgggacca tcggcacatc atcataaaac gccacatctc ccgccgctga acccagtgtc
     3301 agaatgatta acacttcaaa cagcgacatc tgccgcacac cgcgtcttcc ggtcattttg
     3361 aggaacaaaa agaccagtac aaaggtataa agactacgta gtgcaacttc gccaaggaaa
     3421 tcaaaaggca ctttatcaaa tgccatacgg tggagatcga acgccttcat ttttatgctc
     3481 ttatcgtggt ttatccgcta aaagcatagc caacagttca tcggcatggc ggcggtaata
     3541 gcgcctttga gataaaaccc gtattattgt gcgctttccg tacgactaaa gtgattttca
     3601 tgacgcaaac atttatcccc ggcaaagatg ccgctctgga agattccatc gcacgcttcc
     3661 agcaaaaact ttcagacctc ggttttcaga ttgaagaggc ctcctggctg aatcccgtgc
     3721 ctaacgtctg gtctgtacat attcgcgaca aagagtgcgc actgtgtttt accaacggta
     3781 aaggcgcaac caaaaaagcg gcgctagctt ctgccctcgg tgaatatttc gagcgtctct
     3841 caaccaacta cttttttgcg gacttctggc tgggcgaaac catcgccaac ggcccattcg
     3901 tgcattatcc caacgaaaaa tggttcccac tgaccgaaaa tgacgatgta ccagaagggc
     3961 tgctcgatga ccgtctgcgc gcattttacg atccggagaa tgaactgacc ggtagtatgc
     4021 tgattgacct gcaatccggt aacgaagatc gtggtatttg cggtctaccg tttacgcgcc
     4081 agtccgacaa tcagaccatt tatattccga tgaatatcat tggtaacttg tacgtttcta
     4141 acggtatgtc tgctggcaat acccgtaacg aagcacgcgt tcaggggttg tccgaagttt
     4201 tcgaacgtta cgtgaaaaac cgcattattg ctgaaagcat cagcttgccg gaaatcccgg
     4261 cagacgtgct ggcacgttat ccagcagtag ttgaagcgat cgaaacgctg gaagcggaag
     4321 gtttcccgat cttcgcttat gacggttcgc ttggcggcca gtatccggtg atttgcgtgg
     4381 tactgttcaa tcctgctaac ggtacctgct ttgcctcatt cggtgcgcat cctgattttg
     4441 gcgttgcact ggaacgtacc gtgaccgagc tgctgcaagg tcgtggcctg aaagatctgg
     4501 atgtgtttac tccaccaacc ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa
     4561 cgcactttat cgattccagc ggtttaatct cctgggacct gttcaagcag gatgccgatt
     4621 atccgtttgt tgactggggc ttctccggca ccacggaaga agagttc
//
LOCUS       DAWDRC010000307         4667 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7883_length_4667_cov_2.644623, whole genome shotgun sequence.
ACCESSION   DAWDRC010000307 DAWDRC010000000
VERSION     DAWDRC010000307.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7883_length_4667_cov_2.644623"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4354..4363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1154 a         1114 c         1123 g         1266 t
ORIGIN      
        1 aagttataaa cccactttca taatggtata cggagattca atgaaaccac atcgctcata
       61 agcacgaatc gcacgcccgt tatctccatg gacatataag cgaagctcaa gtgccttttc
      121 tgatttggct tcttttataa gatgtttgat gagctcatca gccagtccca tccctcgatg
      181 ctccggggcg atataaaagc tttgaatcca ccagtagtca ttaccatgga aatcactcca
      241 ttcttttacg acagaggtat tggcaattac cgttcctgaa gaatcttcca tgacccaata
      301 ttttgaacga gggttttcac tgaacgccga cgatacacca cgctccacag caacaaggtc
      361 aatcactgaa ccttctgatt cttgcgcttc ctgagtggtg aattcgatga ttttattaaa
      421 atcgtcaggc gtagctggcc gaataaagta gttcataagt ctggctctca ttttcgttgc
      481 tgctatacga atcgcccaat accgtacatc aaatcctgtt aaaagtccgc tcctggcaca
      541 tagcagtcct agagacaatg acgagcaatc atggaaggtt ggtgcgagga cgttcaaatc
      601 atctcatgca aaaaatacga aaatcagtaa cggctagaaa tcattcaata catgcactct
      661 cgaaagtgca tcagccaacc gcagaacgtt atttcaaagc ctactagctg ccttactttt
      721 ttcctcaata acacgtcgcc agcaggaaac aacctaatcc ctgttttggg cctcaaagtt
      781 gctaatgata atcattttca ttttgtatag gttgtgctat aaaacatagc cgttgctatg
      841 tttgttctaa ttttgctcac tataggtact aaattatgca ctcgataaaa aaagtaacca
      901 tgctcttggg gggcctcgca ctcacctgct cgatcgcatt tcaggcaagt gcaactgaaa
      961 aattcaaggt cattacaaca tttaccatca tcgcggatat ggccaaaaac gtggcgggag
     1021 atgctgcaga agtctcatcc ataaccaagc ctggtgcaga aattcatgag tatcagccta
     1081 cccctggcga tattaaacgt gcgcaggggg cacaactgat tctcgccaat ggtatgaatc
     1141 tggaattgtg gttccaacgc ttttaccagc atctcaatgg ggttccagaa gtaattgtct
     1201 cttcgggtgt gacgcctgta gggatcaccg aaggacccta tgagggcaaa cctaaccccc
     1261 atgcatggat gtcgccagat aatgctctga tttacgtcga taatattcgt gatgcgttga
     1321 taaaatacga tccggcaaat gcacaaacct accaacgcaa tgccgatact tataaagcca
     1381 agattaccca aacccttgcc cccctgcgta agcagattac ggaactccct gagaatcagc
     1441 gatggatggt caccagtgaa ggggcttttt cttatctcgc acgcgatttg gggctaaaag
     1501 agctttatct gtggccgatt aatgccgatc aacaaggaac accgcagcag gtacgtaagg
     1561 ttgttgatat agttaagaaa aatcatatcc cggcagtctt tagcgagagt acgatttccg
     1621 ataaaccagc gcgtcaggtt gcgcgtgaaa ccggcgcaca ctacggcggt gtgctctatg
     1681 tcgattccct gagcacagaa aacggcccgg taccgacgta tatcgacctt cttaaggtta
     1741 ctaccagtac gctggtacag ggaattaaag ccgggaaaag ggagaaataa tgatgcaatc
     1801 tgcaggcatt gtcgttaatg atgttactgt cacctggcgt aacgggcaca cagcactgcg
     1861 cgatgcatcc ttcacagtac caagtggatc tattgccgct ctggttgggg taaacggttc
     1921 cggaaaatcg acgttgttta aagcgattat gggatttgtg cgtctgacca gcggaaagat
     1981 atctgttctg ggtattccca cgcgacaggc gttacagaaa aacctggttg cctacgtccc
     2041 tcagtcagaa gaggttgact ggtcatttcc tgtactggtg gaagatgtgg tgatgatggg
     2101 ccgctatggg catatgggta tgttgcgtat cgccaaaaag agggatcgtc aaattgtcac
     2161 cgatgcgctg gaacgtgtcg atatggtgaa ttttcgccat cgacaaatcg gcgagctttc
     2221 cggtggacaa aagaaacgcg tctttctggc gagagcgatt gcacagcagg gtgatgtgat
     2281 cctgctggat gaacctttta ctggcgttga tgtgaaaacc gaagccaaaa tcatcagcct
     2341 gttgagggag ttacgcgcgg aaggcaaaac gatgcttgtc tcaacgcaca atctcggatc
     2401 cgtcacgaca ttttgcgatt atacggtcat ggtcaaaggc accgtcctgg caagcgggcc
     2461 gacagacacc acttttacag ccgagaacct ggagctggct tttagcggcg tactgcgcca
     2521 tgtcacactt aacggctcgg aagaaagcat tattactgac gatgaacgcc cttttgtggc
     2581 tcatcgaccg tcagcggtgc agagggaaga aagatgaacg tgctactgga acccttcagc
     2641 tatgaatata tgctcaatgc gatgtgggtc tcggcgatgg tcggcggtct ctgcgctttc
     2701 ctgtcatgct atctgatgct aaaaggctgg tcgctgattg gcgatgcgtt gtcgcactct
     2761 attgtcccgg gtgttgcggg ggcatatatg ctggggcttc cgttttctct gggagccttc
     2821 ttttccggcg gactggctgc gggcagcatg ctgtttctta accaacgcac ccgcttaaaa
     2881 gaagatgcca tcatcggcct gatcttctcg tccttttttg gcctggggct gtttatggtg
     2941 tcattgaacc cgacatctgt gaacattcag accatcgtcc tgggcaatat tctggcaatc
     3001 gatccggcgg atatcctgca actcactatc attggcattc tctcgatcat cgtgcttttt
     3061 ttcaaatgga aagatcttat ggtgaccttt ttcgacgaga atcacgcccg tgctattgga
     3121 ttgcatccgg gcagattaaa actcctcttc ttcaccctgc tgtcggtttc gaccgttgcg
     3181 gcgctgcaaa cggttggtgc ctttctggtg atctgtctgg tggtgactcc cggagcaaca
     3241 gcatggttgt taaccgatcg ttttccccgc ctgctcatga ttgctgtaac tattggcagt
     3301 gtaaccagtt tcctcggcgc gtgggtcagt tactttctgg acggcgcgac aggtggaatc
     3361 atcgtggtgg cgcagacact gctgtttctt ttagcatttg tctttgcgcc cacccatggg
     3421 cttttggcaa atcgtcggcg tgcgcataag gcgctggagg atcgctcatg atggcattat
     3481 tactggagcc actgcagttc acctttataa gtcacgcact gctgatttca ctggtagttt
     3541 ccataccctg tgcgctgctg tcggtctttc tggtcctgaa aggttgggca ctgatgggtg
     3601 acgcgatgag tcatgcggtt tttcctggca tcgtgcttgc ctggattctg gggttacctt
     3661 tggctacagg ggcattcgtc gccggagtat tctgcgcggt ggcgacggga tacctgaaag
     3721 acaacagccg aatcaagcaa gatacagtga tggggattgt tttttctggc atgtttgccg
     3781 caggtctgat cttgtatatt gcagtcaaac cagacgtaca tcttgaccat attcttttcg
     3841 gcgacatgct cgggataacc atcggcgata taatccagac ggtgattatt gctgggctgg
     3901 ttacacttgt tattagcgta aaatggcgag attttttgct gttcagcttt gattatcaac
     3961 aggcgcaggt aagcggtttg catacgagat ggctgcatta tgggctgctg tgcatggtct
     4021 ctctgaccat tgtggcgacg ctgaaagcgg taggtatcat tctctccatt tctctgctca
     4081 ttgcgcctgg cgccattgcg gtactcctga cacaacgctt ttatattgcg ctcctactgg
     4141 cgactggcat atcggtaata gtgtcaatga ccggtgtctg gctgtccttt tttatcgaca
     4201 gcgcaccagc tccgacgatt gtcgtcttat tcgcggtcgc gttcatcatg acgtttaccg
     4261 ttaccagcat caacgcacgc acaaagggaa acgcccatac acaggatctg ttatcaccca
     4321 attagcacaa catccgagga catcatcgtc cgcnnnnnnn nnntaaaatc gataatagct
     4381 ggaaataatt caatacatgc actctcgaaa gtgcatcagc caaccgtggc acgtttttgc
     4441 atacaacgag ccacggttct ctttacgatt tatagctgta ttggtgaact atgagcaatt
     4501 ttcgcgttat attccaatct ttaatctctt aacggaacaa gttgcatgca tgactatatc
     4561 aagacaactt ataactacca aaacatttgc tttgcaagca aaaacgggga ccattgactc
     4621 taagaaaaat ccgatctatt gttaggatta ttaaagttac ttaacag
//
LOCUS       DAWDRC010000308         4637 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7937_length_4637_cov_2.351375, whole genome shotgun sequence.
ACCESSION   DAWDRC010000308 DAWDRC010000000
VERSION     DAWDRC010000308.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7937_length_4637_cov_2.351375"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    229..238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3862..3871
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1174 a         1137 c         1181 g         1125 t
ORIGIN      
        1 ccctacaacg caacacaatc gtaggcctga gaagacgcag cagcgttgaa ccgggcaatc
       61 agcatcaaat agttccactc aaaacgtcaa actactttca cctaaaatac ccttatcacc
      121 ttatatacag tgaacacctt acctggcgcg aatttctttg aataacggcc taccgccccg
      181 cttatttgcg catcttttgt ctcgatatac attttctgac atcctcatnn nnnnnnnncg
      241 ctatgctaca ggcactaaaa atggttattg aggagcaaat atggctaact ggttaaatca
      301 actgcaatcc ctgcttgggc aaagccgttc ttctacctcc tcgtctgcgg atcagggatt
      361 gggcaaactt ttagtgcctg gcgcattagg cggactggct gggctgctgg tcgcaaataa
      421 atcagcacgt aaacttctta cgaaatatgg cacaaacgcg ttactggttg gcggcggagc
      481 ggtagcgggt acggtgctgt ggaataaata caaagataaa attcgcgcgg cgcatcagga
      541 tgaaccgcag tttggcgcgc aaagtacgcc gctggatgag cgtacggaac gtttgatcct
      601 tgcgctggtc tttgccgcta aaagtgatgg tcatattgat gccaaagaac gtgcggcaat
      661 cgaccagcaa ttgcgagaag ccggtgtgga agagaagggg cgtgtactca ttgagcaggc
      721 aatcgaacaa ccgctggatc cacaacgcct ggctaccggg gtccgcaatg aagaagaggc
      781 gctggaaatc tatttcctga gctgcgcggc tattgatatt gaccatttta tggaacgcag
      841 ttacctgaat gcactgggtg atgcgctgaa aatcccccag gacgttcgtg aaggcattga
      901 acgcgatctc gaacagcaaa aacgtacttt ggcggagtaa ttccgctgtc tggatagcct
      961 gaagttgact atttcgggtt atccagaacc gcacagccac ttgaacaggc tggttttctc
     1021 tgtctgcata acagtcgata tccgcgccat tacctgtcat catctaagca atgactaccc
     1081 tgtttcgctt gcatccccgg tgagttttgc cacccttata agatgtttca accagaaaga
     1141 acaataacat gctaccaaaa gccgcccgca ttccccacgc catgacgctt catggcgata
     1201 cgcgcatcga taattactac tggctgcggg acgatacgcg ttctcagccg gaagtcctgg
     1261 actatctgca acaagaaaat agttacggtc atcgggtgat ggcctcgcaa caagccttgc
     1321 aggatcgcat cttaaaggaa atcatcgacc gcattccgca aagagaagtt tctgcgccct
     1381 acataaaaaa tggctaccgc tatcggcata tttatgaacc aggctgtgaa tatgctatct
     1441 accagcgtca atcggcgttc agcgaagagt gggatgagtg ggaaacattg ctcgatgcca
     1501 acaagcgcgc ggctcatagt gagttttatt cgatgggcgg aatggcgatt acgcccgata
     1561 acaccattat ggcactggca gaagattttc tttcccgacg ccagtacggc attcgttttc
     1621 gtaatctgga aacaggtaac tggtatccgg aactgctgga taacgttgaa cccagctttg
     1681 tctgggcaaa tgattcctgg actttctact atgttcgcaa gcatccggtg acgctgcttc
     1741 cttatcaggt ctggcgtcac gccatcggta cgccagcatc gcaggataaa ctgatctacg
     1801 aagaaaaaga cgatacctat tacgtcagcc tgcataaaac gacctcgaag cactatgtag
     1861 tcattcatct ggccagcgcc accaccagtg aagttcgcct gctggacgcg gaaatggccg
     1921 atgccgagcc gtttgttttt ctgccgcgcc gcaaagatca cgaatacagc cttgatcact
     1981 accagcatcg gttttatctg cgttccaacc gccacggtaa aaactttggc ttataccgta
     2041 cccgtatgcg tgatgagcaa cagtgggaag agttaattcc gccacgtgat aacattatgc
     2101 tggaagggtt tacgctgttt accgactggc tggtggttga agagcgtcag cgcgggttaa
     2161 ccagtttgcg gcaaattaac cgcaagaccc gggaagtcat tggcattgcc tttgatgatc
     2221 cggcctatgt gacctggatt gcctacaatc cagaacctga aaccgcgcga ttgcgttatg
     2281 gttattcgtc catgaccaca ccagatactt tgtttgaact ggatatggat accggtgagc
     2341 gtcgtgtatt aaaacaaacg gaagttcctg gttttgatgc ggcgaattac cgcagtgaac
     2401 acctgtggat agtcgcccgt gatggcgtcg aagttccggt ttcgctggtc tatcatcgca
     2461 aacattttcg caaaggacac aacccgctgc tggtgtatgg ctacggttct tacggcgcaa
     2521 gtattgatgc cgatttcagt tttagccgct tgagtttgtt ggatcgtgga tttgtctacg
     2581 ccattgtcca tgttcgtggc ggcggtgagc tggggcaaca atggtacgag gacggtaaat
     2641 ttctgaagaa gaaaaatacg tttaatgatt atcttgatgc ctgcgatgca ttgttaaaac
     2701 tgggctatgg ctctccttcg ctctgttatg cgatgggcgg gagtgcgggg ggtatgttga
     2761 tgggcgttgc gattaatcaa cgcccggaat tgttccacgg cgttatcgcc caggtaccgt
     2821 ttgttgatgt tgtaacaaca atgctggatg aatcaattcc tcttaccacc ggcgaatttg
     2881 aagagtgggg gaatccgcag gatccgcaat attacgagta tatgaaaagc tacagcccgt
     2941 atgacaacgt caccgcacag gcttatccgc atttactggt aacgaccggt ttacacgatt
     3001 ctcaggtgca atattgggaa ccggcaaaat gggtcgctaa attgcgcgag ctgaaaaccg
     3061 atgaccatct tttattgctc tgtaccgaca tggactcagg tcatggcggt aaatctggtc
     3121 gctttaaatc gtacgaaggc gtagcgatgg aatatgcttt tctggtcgcg ctggcgcagg
     3181 gaacattacc cgctcagtca gcggattaag tattttccag ataatgtttc agtgttaaac
     3241 gcagctccgg gctcatgctg tcgaggttat taaacaacca gcgcagatag cccggatcgc
     3301 gttcggcaac gtcggaaacc gctttgccac ggtatttgcc aaaggtgaag gtcgtcatca
     3361 acgatggacg tccggtgata tcggccatct gttctgccgt ccagccagag gtgttcataa
     3421 tatcgataag caacgcggcg gtgatataac agtcatacaa cgcgcggtga tgatgcaggc
     3481 ccggcggcgt ctgtacattg agcttccgtg ttttatatag cgccatattg ctgtacttga
     3541 tccctggcca caaacgacgg gccagtttca tagtgcaaat ccactcaccg ggcatttcag
     3601 gcagtacgcg gcggtcaaag ctggcgttat gcgcgacata ccattcacta ccgtaatagt
     3661 gcgggatcac atcttcaatc cacggtttat cggcgaccat ggcttcggtg atgcgatgaa
     3721 tcgccatcgc ctgtggacta ataggacgat cgggacgcac caggtggctc atgggattga
     3781 cgatttttcc gtcaatgaca tcaacagagg caatctctac gatccctccc tgcaaaccgc
     3841 aggtttctgt atcgataatg cnnnnnnnnn ncgataatgc gcaacatggc atgctccagg
     3901 ccgaaaagcc tagcgtaatg gaatgatatc gccttgccaa ccctgtgatg aacgctgacc
     3961 gactaacaat aatgaaccac gatcggcgcg aacgatgaga cgaccatgtt catcccatag
     4021 tgcactgtta ccacgggcat ttgccatcag cactgcaatc gagtactgat gtgaaaaaaa
     4081 ttgtaagcga cgggccgacg ccagcaggtc gggttctccc agacattgcc ctgtcgtaaa
     4141 cagtgaacag gtcgggtcca tatccatacc ttgcggttgt tcatcgacca ccgtgatggt
     4201 tctggaacga cggcccagac atgcaccata actctgatgg tagatccctg gcgtttttcg
     4261 ccacggtgcg aataccgcaa ttccacgaat aaagcgatcg ttatattcaa cgggaaggcc
     4321 ggcaatgatg gtcattcgcc aggtcgttgc ggcataacaa agcgggtcta atagcgagag
     4381 atcgggtggg gcaggaaggg cacgtcgcga ataatcacac cccagtaaag aaagcgatgg
     4441 aaaaactaac agctgacatt gttgcctggc ggcggccctc acaaactcca gatgatgagc
     4501 tacctgttcg gtgagtgagg ttttacgggg ctcatattgc gctgctgcaa ctttccagaa
     4561 cgacataatg acttcctttt caaaatgact gcaaattatg ggggcggttt agaaaatact
     4621 cataatttag caggcat
//
LOCUS       DAWDRC010000309         4632 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7945_length_4632_cov_2.013328, whole genome shotgun sequence.
ACCESSION   DAWDRC010000309 DAWDRC010000000
VERSION     DAWDRC010000309.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7945_length_4632_cov_2.013328"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    301..310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3544..3553
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1181 a         1172 c         1172 g         1087 t
ORIGIN      
        1 agatccgcgt gccatagaga atgcgtgcca gtacatcgcc gccattggca tccgttccca
       61 gccagttttg ccgggagggt ggagaaggga agggcttatc ggtagcaaag ttgatactgg
      121 tagcatcaaa gcgaatcgga gcccacagta cccagccgtt attttccagc cgttgtttca
      181 gccacgggtc ctgataatca gcctgacttg ccagcgggcc gccaaaatcg ctttcgctgt
      241 agtttttcaa caacgggaaa taccaactgc cgtcataacg caccagcaac ggtttatcgt
      301 nnnnnnnnnn tcgttggcga gaagttcaga acacaaactc aaaccaaaca agacgaggaa
      361 aatccataac gaccagtagc cgcgacggtt atggcgaaaa cgcgcccaac gggcctgatt
      421 gacggggctg agtcgcgaca ttaacgtccc tcaaaatcta tacgcggatc gaccagcgta
      481 tagctgatat cactgacaat attcagcagc aggccaatca gggtgaaaat atacaaggta
      541 ccaaacatta caggataatc gcgcgagacg gtcgcttcgt agcccagtaa gccgaggcca
      601 ttgagtgaaa acatcacctc aatcagcagc gagccggtaa aaaacatgct gataaacgtc
      661 gccggaaaac cggcaatcac cagcagcatg gcgttgcgga acacatgttt ccagagaata
      721 tttttttcac ttaccccttt cgcacgtgcc gtcaccacgt attgcttacg cacttcatca
      781 aggaatgagt tttttgtcag catggtcaga gccgcaaagc cgccaatcac tgtcgccagc
      841 accggcagcg tgatatgcca cagataatcg gtgattttct gataccacgg cagcgaatca
      901 aagttagcgg aaaccaggcc gcgcagaggg aacagatcga aatagctgcc gcccgcgaag
      961 aagacaatca gcaggatggc aaacaaaaag gccggaatgg cgtagccgat gatgataaat
     1021 gcactactcc agacgtcaaa acggctccca ttataaacag ctttgcgaat gcctaacgga
     1081 atcgacacca gatagataat cagcgtgctc cacaatccga gggtgatgga gaccggcaga
     1141 ctgtctttaa tcagcgtcag caccgaggcg ctgcgaaaaa ggctgtcgcc aaaatcaaaa
     1201 cggatgtagt cccagagcat tttgaagtaa cgttcgtgga tcggcttatc gaaaccgtag
     1261 cgatgagtga tctcggcgat cacttctgga tctaatccgc gtccgccacg gtaattactg
     1321 tcgctgatat tgccgacgcc cgtttgcgca tggctggcgc gaacaccttc accgcctgcg
     1381 ccgggtaata ctccggcatt gccaaactca atggcggcga tggcctggtc gaccggaccg
     1441 ccaggcgcaa tttgcacgat gaaaaagttg atagtgataa tcgcccatag cgttgggatc
     1501 accagcaaca gacggcgaat cagataagcg cccatctact ctccctgttg tctggctgac
     1561 ggcagtttcg tcgctttatt aacgtcatac caccaggtat caatacccag gctgtaaaca
     1621 gggcgtacag cgggttggga gaatttatcc caccaggcga gatggtcttc cgccatgtac
     1681 cacattggca gcatgtaata attccacgtt aatacgcgat ccagtgctcg tcccaacggc
     1741 agtaattttt ctttatttcc ctgcgcggca ataatttggt tgatcagcga gtcgataacc
     1801 gggctttgca cgccgggggc attataagtg gaattgatat attccgatga ccaggaaatc
     1861 tgtaaatcgg aactgggcca cggcatcgcc cgccatacgc gcggcatcat gtcatagtcg
     1921 cgactgcgca tgcggttggt gatttgcgag ttatccacct tgcgaatgtc catgttgata
     1981 cccagccgtt gcaggctgtg ctggaacggc aatacccact gactattgct gcttgcggga
     2041 agcaataatt caaagctgag tggctgaccc gttgtgacat taacgcgttg ctgacccttc
     2101 agcacccagc ctgcttcgtt gagaagtttg tcggctttta acaggttgtc acgatcgtag
     2161 ccatcgcctt tggataccgg cggctggtag atttgcgtga agacttctgg cggtagatct
     2221 tttttcattg gtgccagaag caccagttcc gcggcgtcgg ggtaatttct ggccgcatat
     2281 tcggtattct gaaaataact gttcgtgcga ctccaggcat tgtaaaacaa cgctttgttc
     2341 atccattcaa agtcaaaggc gagagtgatc gcttcccgca cccggcgatc gctgaatacc
     2401 ggacgttgga tattaaacgc cagccagcgc gtatcctggg ctgattcgtt cttttgctca
     2461 tctttgatga tgtatttttt atcgaaattt ttaccggtat agcgcgtggc ccagtttttg
     2521 gcgtcgtttt ccatacgcaa atcaaaggca cctgctttaa acgcttcaaa ggcgacatta
     2581 tcatcgaggt aataatcgta gcgaatggtg tcgaaattcc agcgtccacg gtttaccggt
     2641 aagtttgctg cccagtaatc tttcacgcgg gaatagacaa tattttgccc cattttccag
     2701 gacgtaatgc ggtacggacc actggcaagc ggaggcgtgg cgagcgggtc gctaagtttg
     2761 tgatctttcc agtacttttc tggaaatacc ggcagcgaaa acagactcag catatcttct
     2821 ttgccgggtt tagctaactc aatgcgcacg gttaacggcg caatggcttt gacggtggtg
     2881 cctttgtaga ccagacgaaa ttgcggcacg ccttcggtca taaatttttg aaaagtaaac
     2941 tctacatcgc gggcagtaat gggcgaacca tcatgaaaac gggcgcgtgg attaatagcg
     3001 acctccaccc aggaataatc ttcagcatag cgtgcgcttt cagcaatcag cgggtaataa
     3061 ctgcctggtt cgtcatcgga agtcgtaaat agcgtgtcgt acagctgctc ggtgcgtgcg
     3121 cccgggttgc cgcgtagtgc atagcggttg aaattatcga aggtgccgag ggcagacaac
     3181 gttatttgcc caccttttgg cgcggcgggg ttcacataat caaaatggtt gaaattaaat
     3241 gcgtaccggg gttcgcccag tacggcaaag gcatagcttt ccttgatagc ctgcgcctgc
     3301 gcaccaaagg tgaacagagc gataaacagc agcagtatgc gcacaatcat ctgcaacgga
     3361 tttcctttta ctcatcatta gccgcaatcc ggcatgcttt cactatgaat aataagggac
     3421 gtaagcacac cttaccactg cggcgtatac ggtttcttca gccagcgaac aaaatcgtcc
     3481 agcggtaacg ggcgactaat ccagtagcct tgcatgaaat taacgccgcg ttcgcttagc
     3541 catnnnnnnn nnntggcgtt tcgacccctt cagcaactgt cagcatattg agacgtttcg
     3601 ccagcgtcag caccgcgtca agtacgggtg aagtgatcgt ttccgtaccg atggcgttga
     3661 taaatccacg atcaattttc agataatcga gcgtaaaacg ctcaagatag ataagcgcgc
     3721 tgtgcccggt gccgaagtca tcaatagcaa tttctacgcc gaccgaatgc agccaggcga
     3781 agagttgtgt ggcttctcgc tctttcagca tatcgcgctc ggtaatttcc agcacaatct
     3841 ggaaatggtg tgcgggcagg gaagtgagca gtttctggat atccgcttta aagctttcgc
     3901 tgtgcaagtg ggccggcgca atgttaatgc caaatttgac gcctaccggc agtacttttt
     3961 ctaattctgc ggcatcgcgg gcaatcaact caaacaggtg ctgagtcagt ggcacaatca
     4021 tcttttgcgc ttcggcaaag ttaatgaagg catccggggg gatttcacct gctactggat
     4081 gccgccagcg tagcagtact tccaggcccg ttacccgcaa agcttgtgta tcaaccaccg
     4141 gttgatacac cacgtaaaat tgttcgcgct tgatggcggt catgatttct ctgcctgggc
     4201 gcatacgcac actcatcagg taatagcaga gaagtccaac gaaagttcca ctcatgccac
     4261 ccagtaaaaa tgcgtaccac acatcgttcc atgcccagtc atctgcataa agccgaacag
     4321 tcagaggaat gcccgcaatt ttagtttcac ggaccggcat gtcggttaat tcattaacgt
     4381 tcatcaaacg tgaagaaaag gtagatagcg cagtattacc aataatgagg gctaggccat
     4441 cgtaatcttc ctgccgtgaa ctataaaaga gtgaaggcgt caggttgaga ttcagagcgg
     4501 caaacacgcc gctatttttc agcaaagggt tgcgatacca gatgacgatt gcgggtttgt
     4561 tcggcaccat cggcgtgccg ggtaagatcg ccatatcgac gtttttatta atgtcgagcg
     4621 ccggaatcaa tt
//
LOCUS       DAWDRC010000310         4631 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7949_length_4631_cov_2.479240, whole genome shotgun sequence.
ACCESSION   DAWDRC010000310 DAWDRC010000000
VERSION     DAWDRC010000310.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7949_length_4631_cov_2.479240"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1223 a         1156 c         1005 g         1247 t
ORIGIN      
        1 cattaatgtt gtgtttagcc cattcgttcg ccatcaatcg cgtcacgccc atcacaccgc
       61 tttttgatgc ggtataagaa ggcacacgga tcccgccctg gaaggagagc attgacgcga
      121 tattgataat cttgccgcca ttgccttgcg cgataaagtg tttcgccgct gcctgagaca
      181 tgaagaatac gctcttgata ttcaggttca tgacatcgtc ccagtctttt tcgctgaatt
      241 cgagagcgtc ttcgcggcga atcaatccgg cgttattcac caggatatca atatgaccaa
      301 actcagctac cgcgcgatcc agcagtgctg gaatgccatc aatctttcgc agatcggcgg
      361 tcaggcttaa aaaacgacgc cccagcgccg tgacctgctt gatggtttca gtcggttcaa
      421 cgatgttaat gccaacaatg tcacagcccg cttgcgccag ccccaacgcc atcccctggc
      481 ccagcccagt atcacaacca gtgacgaccg caactttacc ttcgagagaa aatgcactta
      541 aaatcataac aataccttat tcgtttatgc ccacaactag cgcaaatctt taacggccac
      601 atggtccata tcatcaaaaa cctggttttc accgaccatg ccccagataa aggtataagc
      661 tttggttccg acgccggaat ggatcgacca gctcggggag atcaccgcct gctcgttatg
      721 catcacaatg tgacgcgttt cttgcggttg ccccatcatg tggaaaacgc aggcgtcatc
      781 atccatattg aaatagaaat aaacttccat ccggcgctcg tgggtgtgac acggcatggt
      841 gttccacaag ttacctggag ccagctccgt cagccccata ctcaattggc aggtttccag
      901 cacatccggg acaaaatatt tgttaatcgt gcgacggtta ctggtgaggt tatcgcctaa
      961 cgtgactgga gatacttcgt ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg
     1021 tgcgcaattg taataaaact tcgccggagt ggcggtatca atactggcaa agacaacttc
     1081 ttttgcacct ttaccaacat acagggcgtc gcggtgaccg atttcatagc attgaccatc
     1141 gacagtaatc gtaccggcac cgccaatatt gataacacct aactcgcgac gttcaaggaa
     1201 atagcttacg ccgagttgtt taccaacttc cccgccaacg gaaaccgttt tagttaccgg
     1261 cataatgccg ccaacaataa tacggtcaat gtggctgtaa accatggtgt actcatcggc
     1321 gacaaatacc ttttcaacca aaaattcatt gcgcagcccc tgggtatcca gcgtttttgc
     1381 gtgcgcactg tggatgctct gtcttacgtc cacatcaacc tccgataatc agtgaagcgt
     1441 gaaaatagaa aacaataata aaacactgtt tcgttttctc tatgagggga tgatatgccc
     1501 atgagatagg gttttcaatt aaaaataaaa caatgtttca ttttcaagtt aaggattaaa
     1561 aaaagtgtag tgtcgatcac gaatatgtca tgagaatgga aaaatcaggg agccctgaaa
     1621 aggtatgtcg actcaccagc catcattatt caacagatta atgacggcag atatttatcg
     1681 gtaaggagga aatgcagagc tactcgtcac gttcaatggt caatgccaca ccctggcccc
     1741 cgccgataca aagcgttgcc agtcctttgc gggcattacg tttcaccatt tcatgaacca
     1801 gagaaaccag gattcggcaa ccggaagcgc ctatcgggtg accgagtgcg atcgcaccac
     1861 cattgacatt gacccgacgc tcatcccatt caagcatctt gccaaccgaa agcgcctgtg
     1921 cagcaaacgc ttcattagcc tcgataagat cgacatcagc caactgccag ccaacacgct
     1981 ccaggcaacg acgggtcgca tacaccggcg caattcccat caatgccgga tccacaccaa
     2041 cgctggcaaa tgcgcggatg cgggccagca cgggtaaatt caacgctcgt gctttggctt
     2101 cgctcatcat cattactgca gctgccccat cgtttatgga tgatgcatta cccgctgtca
     2161 cagaaccgag actatcaaat gaaggattta aacgggctaa gccttctgcg ctggtgtcag
     2221 tgcgtggctg ttcatcggta tcaacaacca acgtctgacc gttactttgg gttattaccg
     2281 ggacgatctc atctttaaat cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc
     2341 taagtgcgta agcatactgc aactgacggc tgatgccata ttcgcgagcc agattttcgg
     2401 cggtgacacc aatatgataa tcattgaagg catcccacaa cccatcatgc acaagactat
     2461 caaccaactg gctattgcca agctgtgcgc cggtgcggct atcagtcaga acatgtggtg
     2521 cgcggctcat gttttcctgg ccaccggcga tgacaatatc agcctcgcca cactgtatcg
     2581 cctgagtagc cagatgcagt gctttaagcc cggaaccaca aacgtcatta atagtgattg
     2641 cagaaacgct attaggaaga ccacctttaa tagccgattg ccttgccgga ttctgccctg
     2701 cccctgcagt caacacctga ccaagaatca cttcatccac cgcatatgca ggaacgccgg
     2761 tacgttctat taacgctttg acgaccaggc tgccaagttc cacggcggaa tgacccgcta
     2821 acgcaccacg aaagcagccg ataggtgtcc gtaacgcccc gacaatcaca acgtctttca
     2881 tcacaacctc gcaccaatta acagtgcaat agtaaatgat tgttaattac agttatctta
     2941 attgtttaaa aaaggtgatt tttatcacaa aggaaatacg cctgagtagc agtcagagac
     3001 ataactggca cgtaaggttt gcaaccacta acccaccaat agagaggtag atagggcgtt
     3061 aatctcccat acttaacctg gtttatggta aattgccctc cattttgttt aatttgtaga
     3121 tgatacgttc agataatgtc taatatttgg tcaaaagaag aaactctgtg gagtttcgcg
     3181 ctctacggca cagccgttgg tgcaggcacg ctcttccttc ctattcagtt aggttcggcg
     3241 ggggctgtgg tcctgtttat tactgctctg gtcgcctggc ctttaacata ttggccacat
     3301 aaagccttat gccagttcat cctctcatcg aaaacatcag caggtgaagg gataacgggc
     3361 gcggtaacac actactatgg caagaagatt ggtaatctga ttaccacgct gtacttcatc
     3421 gccttttttg tcgtcgtgtt gatatatgca gtggcaatta ccaactcact tacagaacag
     3481 ctggcaaagc atatggttat tgatcttcgc atccgtatgt tggtgagtct gggggttgta
     3541 ttaatcctga atctcatttt tctgatggga cgccatgcca ctattcgggt aatgggattt
     3601 ttggtattcc cattgattgc ctatttctta tttctttcta tttacctggt cggtagttgg
     3661 caacctgatc tattaacaac ccaggtagag ttcaatcaga atacccttca ccagatatgg
     3721 atatcgattc ccgtgatggt tttcgctttt agccatacgc ccattatttc tacgtttgcc
     3781 atagacagac gtgaaaaata tggcgaacac gctatggata aatgcaaaaa aattatgaaa
     3841 gtcgcttatc tcatcatctg cataagtgta ttgttctttg tctttagctg cctgctttct
     3901 attccacctt cgtatattga agcggctaaa gaagaagggg ttaccatttt atcggcgctt
     3961 tctatgctgc cgaacgcccc agcatggttg tcaatttccg ggattattgt cgcagtagtt
     4021 gcgatgtcga aatcattcct gggtacgtac tttggcgtta ttgaaggtgc cacagaggtc
     4081 gtcaaaacaa cattacagca ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta
     4141 tcaattatgt tggtatcgct gattaccttc attgtttgtt gcattaaccc gaacgcgatt
     4201 tcgatgattt acgcgatcag cggcccgctc attgccatga tacttttcat catgcctacg
     4261 ctgtaaacgt atctcatccc ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacc
     4321 ctgatcgtgg gtatcctgtg cgtatcggta atgttcttta gctaatatcc gttgtgaatg
     4381 gttcaaaatt gcttcaattt tgaaccatcc cagttttcat aatgttcatt tgttattatt
     4441 ccgctctatc acttctctcg taatattata taattatcag ctagttaaac acacaaagag
     4501 ttctgaacat cttaaataag actatttaag atacatgacg attgcattgc gtaaacgctt
     4561 tttatttaca aaaaaatggg gaagtattac ggcgattgcg aaaagtctga agcgcttgaa
     4621 ttaagataat a
//
LOCUS       DAWDRC010000311         4608 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_7999_length_4608_cov_2.141884, whole genome shotgun sequence.
ACCESSION   DAWDRC010000311 DAWDRC010000000
VERSION     DAWDRC010000311.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7999_length_4608_cov_2.141884"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    266..275
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    613..622
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1185..1194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1069 a         1159 c         1233 g         1117 t
ORIGIN      
        1 aaccgccgag caactcaggc agcatcggcc cgtaagcgtt aagcgtattt tgcgaagcct
       61 tacgggtagc gattttcgcc ggattcgcct gcaactcatt gatatatttc tgagtcgttt
      121 tctcccagtc cttcggcaaa ccgccgctca tccgacgggt aaactcttct gccagttgcg
      181 gatgagcctt tttataggcg gcaaacttct cattccagct ctgctgcgct ttttcgcctt
      241 tttcgcgggc atcccaggcg tgatannnnn nnnnngtggt gcgccgtgcg cctcttcctt
      301 acctgcttta ttcggcgaac caaagccaat caccgtacgg cagataatca gcgacggctt
      361 atctttcacg ctttgcgctt caaggatcgc ttccttcacc gcctgcggat cgtggccgtc
      421 gatctcgtgg accacatgcc agtgataggc ttcaaaacgt tttgccgtat cgtcggtaaa
      481 ccagccttcg gtttcaccgt cgatggaaat accgttgtga tcgtaaaaac caatcagctt
      541 gcccagcccc agcgtgcccg ccagcgaaca gacttcgtga gagatacctt ccatcaggca
      601 gccgtcgccc atnnnnnnnn nncataggtg aagtgatcga caatttcatg atccggctga
      661 ttaaactgcg ccgccagcgt acgctctgct atcgccagcc cgacggcgtt cgccaaacct
      721 tgtccaagag gaccggtggt ggtttcaaca cctggcgtat agccgatttc cgggtgacca
      781 ggggttttcg aatgcaactg acggaagttc ttcagttctt ccagtggcag gtcgtaaccg
      841 gtcagatgca gcaaactgta gagcagcatc gacgcgtgac cgttggaaag aataaagcgg
      901 tcacggtcat accaggttgg gtcggtgggg ttatgtttaa gaaaatcgtt ccacagcact
      961 tcggcaatat cagccatgcc catcggcgcg ccgggatgac cagaattggc tttttgtacc
     1021 gcatccatac tgagtgcgcg aatcgcattg gcaaggtctt ttcgggacat ataacactcc
     1081 gtggctggtt tatagtttgg cggcaagaag atcttccagt ttgcgttgat caacggcgaa
     1141 cagacgaatg ccttcagaca gtttttccgt tgccatggca tcatnnnnnn nnnngattaa
     1201 tttacgtacc actggcgaaa ctttttcctg tagctccttc agcaaattcg gtgcgatagt
     1261 cagtcggtcg cagccggtta aggcgaggat ttgttcggtg cgacggaagc tcgcgcccat
     1321 cacaatggtt tcatagtggt gttgcttata gtagtcgtag atattgcgta ctgatttaac
     1381 gcctggatct tcttccacca catacgggtc catcggcttg cgtgcctgat accagtcata
     1441 aatacgcccg acaaacggcg aaatcagaaa aacgcctgct tctgcacagg cccgcgcctg
     1501 tgcaaaagaa aacagcagcg tcaggttgca gttaatacct tctttttcca gctcttctgc
     1561 cgcgcgaatt ccttcccagg tcgaagccag cttgatcaga atgcgtgatt tctcaacgcc
     1621 ttgttgctga tacaagtcca ccagatggcg cgccttctca atactctttt ctttatcaaa
     1681 agagaggcgt gcatcaactt ctgttgacac gcgaccgggt acgattttga gaatttcagc
     1741 accgaaattg accgccagtt tgtcacacgc tgcgaccacc tgttgttcct gggtcttgcc
     1801 atttttttta ccccaggcga tagcatcgtc tattaaatgc tcatattgtg ataatccggc
     1861 agccttgagt aacagcgaag gattggtggt ggcatcctgg ggatgataat ggcgaatgga
     1921 ctcaatatcg ccgctgtctg ccacgacagt ggtgaactgt ttgatgccgt ctaactcgtt
     1981 cataggaaat actccttgaa aagtaaagtg ttagatgagt gcgttaattc acacttctga
     2041 gaaatttcgc taaacgcatc aaaaaagcat agcagacagg catggtattg ctggattaag
     2101 caggtaacat cagtgttata ggattattac caaaacatta tattaatggt gagagtttgg
     2161 acttgctcaa agtctgtaga ctccggcagg gtaataatgt gcgccacgtt gtggacaggg
     2221 gctgttgccc acacacttta tttgtgaacg ttacgtgaaa ggaacaacca aatggatgac
     2281 cagttaaaac aaagtgcact tgatttccat gaatttccag ttccagggaa aatccaggtt
     2341 tctccaacca agcctctggc aacgcagcgc gatctggcgc tggcctactc accaggcgtt
     2401 gccgcacctt gtcttgaaat cgaaaaagac cagttaaaag cctacaaata taccgcgcgc
     2461 ggtaacctgg tagcggtgat ctctaacggt acggcggtgc tggggttagg caacattggc
     2521 gcgctggcag gcaaaccggt gatggaaggc aagggcgttc tgtttaagaa attcgccggg
     2581 attgatgtat ttgacattga agttgatgaa ctcgacccgg acaaatttat tgaagtcgtc
     2641 gctgcgctcg aaccgacctt cggcggcatc aacctcgaag acattaaagc gccagaatgt
     2701 ttctatattg aacagaaact gcgcgagcgg atgaatattc cggtattcca cgacgatcag
     2761 cacggcacgg caattatcag cactgccgcc atcctcaacg gcttgcgcgt ggtggagaaa
     2821 aacatctccg acgtgcggat ggtggtttct ggcgcgggtg ccgcagcaat cgcctgtatg
     2881 aacctgctgg tagcgctggg tctgcaaaaa cataacatcg tggtttgcga ttcaaaaggc
     2941 gttatctatc agggccgtga gccaaacatg gcggaaacca aagccgcata cgcggtggtg
     3001 gatgacggta aacgtaccct cgatgatgtg attgaaggcg cggatatttt cctcggctgt
     3061 tccggcccga aagtgctgac ccaggaaatg gtgaagaaaa tggcccgtgc gccaatgatc
     3121 ctggcgctgg cgaacccgga accggaaatt ctgccgccgc tggcgaaaga agtgcgtccg
     3181 gatgccatca tctgtaccgg ccgttccgac tacccgaacc aggtgaacaa cgtcctttgc
     3241 ttcccgttta tcttccgtgg cgcgctggac gttggcgcaa ccgccatcaa cgaagagatg
     3301 aaactggcgg cggtacgcgc gattgcagaa ctggcccatg cggaacagag tgaagtggtg
     3361 gcttcagcgt atggcgatca ggatctgagc tttggtccgg aatacatcat tccaaaaccg
     3421 tttgatccgc gcttgatcgt taagatcgct cctgcggtgg cgaaagcggc gatggagtcg
     3481 ggcgtggcga cgcgtccgat tgctgatttc gacgtctaca tcgacaagct gacagagttc
     3541 gtttacaaaa ccaacctgtt tatgaagccg attttctccc aggctcgcaa agcgccgaag
     3601 cgcgtagttc tgccggaagg ggaagaggcg cgcgttctgc atgccactca ggaactggta
     3661 acgctgggac tggcgaaacc gatccttatt ggtcgtccga acgtgatcga aatgcgcatt
     3721 cagaaactgg gcttgcagat caaggcgggc gttgattttg agatcgtcaa taacgaatcc
     3781 gatccgcgct ttaaagagta ctggaccgaa tacttccaga tcatgaagcg tcgcggtgtc
     3841 actcaggagc aggcgcagcg tgcgctgatc agtaacccga cagtgatcgg cgcgatcatg
     3901 gttcagcgtg gcgaagccga tgcaatgatt tgcggtacgg tgggcgatta tcatgaacac
     3961 tttagcgtgg tgaaaaatgt ctttggttat cgcgatggcg ttcacaccgc aggtgcaatg
     4021 aacgcgctgc tgttgccgag tggtaacacc tttattgccg atacctacgt caatgatgag
     4081 cctgatgcag aagagctggc ggagatcacc ttgatggcgg cagaaactgt tcgtcgtttt
     4141 ggtattgagc cgcgtgtggc tctgttgtcg cactccaact ttggttcttc tgactgcccg
     4201 tcgtcgagca aaatgcgtca ggcgctggaa ctggtcaggg aacgtgcgcc agaactgatg
     4261 attgatggtg aaatgcacgg cgatgcagcg ctggtggaag cgattcgcaa cgaccgtatg
     4321 ccggacagcc ctttgaaagg ttccgctaat attctggtga tgccgaacat ggaagctgcc
     4381 cgcattagtt acaacttact gcgcgtttcc agctcggaag gtgtgactgt cggtccggtg
     4441 ctgatgggcg tggcgaaacc ggttcacgtg ttaacgccga tcgcatcggt acgtcgtatc
     4501 gtcaacatgg tggcgctggc cgtggttgaa gcgcaaaccc aaccgctgta attctttttt
     4561 aactctcacg cttaccctga atattcaggg taagcggttt agctgcaa
//
LOCUS       DAWDRC010000312         4602 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8011_length_4602_cov_2.142512, whole genome shotgun sequence.
ACCESSION   DAWDRC010000312 DAWDRC010000000
VERSION     DAWDRC010000312.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8011_length_4602_cov_2.142512"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3269..3278
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1131 a         1167 c         1169 g         1125 t
ORIGIN      
        1 cagtaaatat aatgcatcat atcttcctga tataattacc tttgacttcc cttttatttc
       61 catctggtaa tatttgagtg caaaaaaggc gataataata tcgcaaaaga aaacgttaac
      121 aactatcgaa aatgctcgtt ttaaaaagtt atgatgttct ctgatagtgt gtgaagtgat
      181 atttttgtga tgttttagat atgtaataat aaaataggtt aacgtatgtt gaaatcattt
      241 attgtgtaaa ccactgatag atcataatcc agtacatctt taaacagctg cactgtatcg
      301 ctgataacta agtagctcac tgatacacca tgctggacgc gataattgga atctcacgca
      361 gggagtactg tgtaaatagc aaaatcgcct gatgtgctac gactgtcagg cctacaaata
      421 acatacaatt catcgaatta tcataacgtt gtaggtcgga taagacgcgc cagcgtcgca
      481 tccgacataa ataaagcgca tttttctaac aacctgtggg ggaatatagc cgctttattt
      541 caggcggtta cccgcctcat caaccacttt ctcgccatct tccttggtga acgcgccttt
      601 ttgcgtatct ggcagaattt ccagcacgac ttctgaaggg cggcacaggc gagttcccag
      661 cggcgtcacc acaatcgggc gattaatcag aatcgggtgc tgaagcataa agtcgattaa
      721 ctgatcgtga gtaaatttat cttctgcaag gcccagttcc tcatacggct cgacgttttt
      781 acgcagcaac gcgcgtacgg taatccccat atcggcaatg agttttacca gctcatcgcg
      841 cgtcggtggt gtttccagat aatagataat ggtcggttct gtaccgctgt tgcggatcat
      901 ctccagcgta ttacgcgacg taccgcaggc cgggttgtga taaatggtaa tgttgctcat
      961 atcagtatct cattacgatg tgaaagagag gtgtagcgcc agcgccacca gagttacaaa
     1021 ccaaagcgga caagtcaaaa acacatatgg tttaacgaat atgtaagtgt agagaaaggt
     1081 gtaggtgtga attaagcgtt gggcattcat ttgaaaggag gtctgaatca ggacgtaaaa
     1141 aaaccgactt cgcgtcggtt tttttactgt tcagccctga gttggtggct ctggctggag
     1201 tgagatagcc atcatctaac aatgagacat gcgccgtgac aggcaatgga tgagtaagcg
     1261 gatgcattct cactccatcg catggagaaa acgggtgatt gataaagcaa tcatcgttct
     1321 tggggcgtta attgcgcagc tggaactgat ccgctttctg cttcagcttc tgaactgata
     1381 gcggaaacgt agctaagggc taagagcaca ctactcttag ccctttaaca tttaacgcat
     1441 tgtcacgaac tcttctgccg ctgtcgggtg aatggcaacc gtgttgtcga agtctttttt
     1501 ggttgcaccc atcttcagcg ccaccgcgaa gccctgcaac atttcgtcca taccaaaacc
     1561 aatgccgtga ataccgacaa tcttctcttc cggtccaatg cataccagtt tcatgcgaca
     1621 cggctggcgg tgagtggtga cggcggtata catcgcggtg aaagaggatt tatacacttt
     1681 cacctgatcg tcgccgtact gctcgcgcgc ctgcggctcc gttaaaccaa cagtaccaat
     1741 cggcggatgg ctgaagacca cggtcggaat gttgctgtaa tccagatgct catccggctt
     1801 gttattaaac aggcgttcag agagacggcg acctgctgca acagccaccg gagtcagttc
     1861 cactgcaccc gtgttatcgc ccaccgcgta aataccttca acgttggtgt tttgatattt
     1921 atcgacgacg atatagcctt tttcgttggt tttaacgcca gcggcttcca ggttgatgtt
     1981 gtcattggca ggctcgcgac caatcgccca aatcaggcaa tccaccgttt cactgcgacc
     2041 atcttccagc tccagcgtca ggctaccatc ggcatttttc actaccgctt tcgggatggc
     2101 gttggtgtgc aactgcgggc cttcggcgtt catcacttca accagcgttt cagagatcat
     2161 cgggtcgaag ctgcgcagcg gtgcatgttt acgcacaaac agatgcgtct tcgcaccgag
     2221 gccgttaatc acgcccgcca gttcaacggc gatgtaaccc gcgccaacaa ccgctacgcg
     2281 ctctggcaaa gccggaaggg cgaagaagcc atcagaatca ataccgtatt ccacgcccgg
     2341 aatatccggg tggctcggac gaccaccggt ggcgatcaga atatgatcgg ccgtgatggt
     2401 ttcgccgttg acttcaatgg ttttggcatc aacgaagcgg gcaaagcctt tgattacatc
     2461 aacgttattt ttaccgagca cgttctcata ggaagtatga atacggtcga tataggcggt
     2521 acggctggcg atcaacgttt cccagttgaa tttattgata gtggtatcaa aaccataatc
     2581 cgggccgtac atatggatcg cttcacggat ttgcgccgcg tgccacatca cttttttcgg
     2641 cacacagcca acatttacgc aggtgccgcc cagctctttg gcttcaatca gtgcgcattt
     2701 ctggccgtac atagccgcgc ggttgatgga ggcgataccg ccgctgccgc cgccgatggc
     2761 gatgtaatca tagtgtttag tcatgacata ttgtccttat cgttgattac cgcgattgta
     2821 gcgcgagacg taataggtgc cagcaatggc tgcaattact ctggcacgat ccagcttacg
     2881 gtggcgtgcc cggtgcctgc cggaaccagt ttgctgtgca gccacggcag cacgttattc
     2941 atctgctgtt ccagcttcca cggcgggtta atcacaatca tgccagaagc ggtcatgcca
     3001 cggcgatcgc tgtctggcag caccgccagt tcaatttgca gaattttgcg aatgccggtc
     3061 gcttccaggt cgtggatcat gcgcttaatt tgctggcgca gcaccactgg ataccacaat
     3121 gcgtaagtgc cagtggcgaa acgtttgtaa ccttctgcta tcccgctgac caccgcctga
     3181 tagtcggttt tcatttcata cggcgggtcg ataaggatta aaccacggcg ggaaaccggc
     3241 ggccgcctgg ccttaagctg ccggaaacnn nnnnnnnntt ttcgacgcgc gcacggctgt
     3301 ctttctgaaa ttcagaacgc agtaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     3361 ggctgtcctg ttcacgcagt agctggcgag caatcaacgg cgaaccgggg taataacgca
     3421 actgcccgct acggttgaag tgttttacca cattgatgta cgcctccagc tccgcaggca
     3481 aatcgtcctg ctgccagata cgggcgatgc cttcgagata ttcgccggta cgctcggcat
     3541 gttcgctgcc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     3601 tatctttctc tttcagcgac tcaatgatca ggctctgaac ggtatgttta aggacgtcgg
     3661 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     3721 gtaaaaatcg gcccgtattc ggggcgcaca aaagtctatc aggacagtat accgaaaaga
     3781 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     3841 acaaacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     3901 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     3961 aacatgtcgt tccagccgtg actaaggcgc tgaacgactg ccgcgaaaat gtggagcgcg
     4021 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     4081 acgatgtgct ggggcgtatc ttctccccgg tcagccatct gaactcggtg aaaaatagcc
     4141 cggagctgcg tgaagcgtac gaacaaaccc tgccgctgct gtcagaatac agcacctggg
     4201 tagggcaaca tgaagggctg tataaggcat atcgcgacct gcgtgatggc gatcattacg
     4261 ccacgctgaa cacggcgcag aaaaaagcgg ttgataacgc actgcgcgac ttcgaactct
     4321 ctggcatcgg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     4381 ctgaactggg caatcagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     4441 tcgttaccga cgaagcggag ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     4501 cccaggccga agcgaaagag ctggaaggtt atttgctgac gctggatatc ccaagctacc
     4561 tgccggtaat gacctactgc gacaaccagg ccctgcgtga ag
//
LOCUS       DAWDRC010000313         4599 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8021_length_4599_cov_2.244058, whole genome shotgun sequence.
ACCESSION   DAWDRC010000313 DAWDRC010000000
VERSION     DAWDRC010000313.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8021_length_4599_cov_2.244058"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1735..1744
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2879..2888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1284 a          997 c          982 g         1316 t
ORIGIN      
        1 tctgctggtt cagcaaggtt ccaggagaga ctacaggaag cttgtaatcg acaatacagt
       61 ttgagttttt atcattgccc catgaaacct gtaattgccc tgactgtgga agtccagtca
      121 gataaacctg accattttcc gcgacaatgc tgccattttt attctctccg tgagtgacaa
      181 ttgcaccgaa tggaacgctt ttattaccgt acttcaacgt cattaatact ttcccgccga
      241 tttgtgcatt aaatgttgct ctggcaatag caccgtgagt tgggatgaca gtgactacgg
      301 tttcatccag ttcaacatta tctgcaaggg aattcgcgtt aagagcgaca cgattttctc
      361 tatattctgt cgcaaatggt aatatggcat agccacgcca gtcggtatga attccggtct
      421 ggttctctat tttgacatta tcagcgccag gagccttaac cagaaccatt gtgtcgccca
      481 gcggctgtcc aaaggtgatg ccatcagcat gagcaataat cccaccactc attccgtaat
      541 aaatctggct gctgtcacca ctccgactgt aaccgacatt cgtattaccg taagctccac
      601 gataattaag agaactgtaa ccactggtgc cagacgatgt attaccccct tgggtgttac
      661 cgacctgaac actataattc aggttattat ccggtagcag agtgccataa acccctgata
      721 ggttggtcat gccgcctttc aaatcgtttg acatactgta actggcgttt gaattatgaa
      781 atgccgactg actgtctgta cgcatccaat gactaaaggg aacattaagc gtaaaagcga
      841 gtaaatgatc ccgatcgttt tgccatatat tattggaata gctgtaattc agcgaagtcg
      901 taatatcacc aaacggcaca tttaatccaa atgatatttg ctggtcgctg cgtgacgtgt
      961 tccagtaact ttggcgactg gcactgaaaa atgtcgtacc gtaatttcca agctgctgag
     1021 agatgcttat ttgttcctga ccacgcttac tgtagaacag attaaaataa tcaataaata
     1081 gtgtctgctc actggtgtct ccggtaggag gcttgacggt gtaaccactc attcgactgt
     1141 atgcactgtc ggataagtta taaaaacctt gcgtagaata gcgatatcct gcgacctgga
     1201 tattggttcc cgtctggtag aaggatttgc tgtagacgga tttaaccgat tgtccgctgt
     1261 gacgggtgtc atctgcaagt gtcgtattgg cctgcgtaat atcgaaagaa aaggcaccaa
     1321 aaagtcctaa atctttacca ataccaaggt tgaaggcctg ataatcttct gcaatttgca
     1381 ttccgccata aggtgtccag tttcctttca gaccatgcat caagcttgct tgtacgaact
     1441 tgggggtgct ttgcaggtta tttccactac gatattcccc catggcaagt gcataacgcg
     1501 tatatccagc acgttggaga accggaacag acgaataagg aacatataat gtctgaatac
     1561 tgccgtctgc ctcttttatg gttacttgca gatcaccacc attggccgca gagttgatat
     1621 catcaatagt aaaagggccg ggtgggacag tagtctgata aacatcgtat ccattctgtt
     1681 ttacactcac ttgtgcggtg ccacgagcaa taccatgaat caccggagcg aaacnnnnnn
     1741 nnnngagcga aaccatgttg gctatcgggc aacatcgctt ctgttgaatt tattttaagg
     1801 ccacgaaagt tcacactgtc aaaaatatcg ccatcggtat aactatcacc cactgttaac
     1861 cgtgagcgta acggaataat gtcacgttca gccgacgtat tgatatgctg ccatttattg
     1921 ctgtcagatg aattgctact cccactgtta taactccacg ttgagttatc gcgcagtcgc
     1981 caactaccaa tgttgatgcc actctgtaaa ttcaaatatg catagttgga ttttcctgca
     2041 ttatggttac tacggttatt aatagaatta ccattaaaac tatagtttag tagcccggca
     2101 tttattcctt cttcccacag atcaggagaa acatagccgc gagccattct gccgacatat
     2161 atttgcggaa cggagagtga cagatgttgc tgaccaacat caaactcaga tgttgcgtca
     2221 gtcaaacgat cctgaagtgg tgtgcatgcg ctgttgtccg gcacatgttt ttctgccgaa
     2281 tgttccttgt tgtctaataa tgctgatttt tttatgccaa gactgacaag aaggtctgta
     2341 ctcaggcatg ggatgagatc tgcgttatta tcatctgcaa taaaagtaat atcccgacta
     2401 gccataaatt cgtcgttcaa ataaatgtcg acccgatatg taccaggtgg ggcctcgcgt
     2461 cccttagtaa aggcagataa atcgacagat tcagcaaggt cattgcttaa aaaccgtgga
     2521 ttaaaataat actctgcgtc aacgggaaaa accgcagttg ctaagccaag ggcaaaggta
     2581 agccgtgcaa agcgaggcag tattaacacc tgatgcatac gatatgcctt aaatgcggca
     2641 cgaaaggctg tcatttttaa tcccgattga gtttattatt aattaggtca ctggcttact
     2701 gcatggttgc cttaatacgc ggagttaatg caccgtaatc attgattgtc tgaaaactga
     2761 tatccccggt tacagcatgc gggatatcca ccgatacttc accttttggc ggcaccatgg
     2821 tgtttgggag attgctattt cctgctttca tatttgttac ggtaatgaag tagggcgtnn
     2881 nnnnnnnnga gtgagtttgc taccgctgcg acgaaaccga agcattgcag gcgcttcttc
     2941 aggtgccatt gccagatgcg tggggcgata aaacattttt atgcggctaa taattgccag
     3001 ctgtaatgta ttctcattct tctggtcttt ttccatagca gggatagctt taacattaac
     3061 ccagaacagg ctttcacggt cgcctggtaa ctgatgatta gtggcgttga ttatccgaag
     3121 cgtattctct tttttacctt gcatggaaaa aagcggtggg gtaatgacaa actgggtatc
     3181 tttctggtca cccgcatttt ctatccatga ctgaatgaga tagacactgg caggatcatt
     3241 attcgtgaca ggcaataaaa cctgtttctg gtttgccgga taaattaccc gcgtcgcacc
     3301 aagtgcgaca gctgcgttgg caatggtgct atggcaagca aagagcaata aaagtaacac
     3361 tgtcgccata aaggaaacag agtagggggt acgcgtagtt tgcatctgat gcattctcca
     3421 tgtgttaaga ggataagcgg ggaaaccgtt acatcagaca atcggtaaat attgctaata
     3481 cattggcgag tcgccgggtt tccagactta atgcgtttgt aataaatgtt ggttctcttt
     3541 tgataggaaa accttgctac caagtaaata ttttaaataa cagcaagttt aactactgtt
     3601 gtttattgat cgtttagata ttatgtgatt tttcataccg ctccaaaata caaaccattt
     3661 ttcattaata atgagatgaa cattggtgaa tttctcgggt gtgcgtagcc atgccacaca
     3721 caccctgttt caatttcaga tgacgcatag tcaccttaag agtgaattat agatattgaa
     3781 ctttgaaggt cgcatcagcg tttgcagtac cagctactga ctgcccgaga gcaatataac
     3841 gagcctggaa cggcagtata ttcgtgccat caatcaagtc agttttagcg ctaaaagtag
     3901 caccatcaag tactaatacc tcccctgtac ggtccaaaat ttggataccg acattttggg
     3961 cagagcctgc cgctgaactc tgcagcgcta atgtatcgtc attactggtg acagtagtac
     4021 ccaggaatgc aacagctgca ttactggaaa cattggtatc acaatcattc agcttgatat
     4081 tgaagccaac ggcggagctg aggtcacccg ctttagccag gcgagaagaa cgaacctgac
     4141 ccagctcaac cgtttggttc attgattctg aatcaatcgc acaagcggca tttaccactt
     4201 cacctttaaa atgaattaca ccgccattaa cagtggtcgt tactgattca tcaccctcgg
     4261 ctgcgcttac cgcagcagac gacatcgcca acacagaaac gacaataata ccgacatgtt
     4321 tgagtttcat aattctccaa aatttatatt taagtagctg aataaagaga gttaacaatt
     4381 aagttattct gttattgctt ataaaacaag aatcattctt gattggatgt tgccctgaga
     4441 atatagtttc catggttatt acatttcacg aagaatgata atatctttaa attacaagga
     4501 cacctattca caacaagttt attgctgcac ttgttgtaga gagtatttgt aaaaacattt
     4561 tatgtcgcta aataacatga cgatttcatt atgcatgtt
//
LOCUS       DAWDRC010000314         4586 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8042_length_4586_cov_2.246745, whole genome shotgun sequence.
ACCESSION   DAWDRC010000314 DAWDRC010000000
VERSION     DAWDRC010000314.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8042_length_4586_cov_2.246745"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    4058..4067
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1122 a         1166 c         1159 g         1129 t
ORIGIN      
        1 ggtcagcaaa gattcgttaa tgctacaact ctatccggat gcggagctgc gggaaagtca
       61 taccatagat gtactgatgg gacgtctgcg caaaaaaata caggcacaat atccgcaaga
      121 agtgattacc accgttcgcg gccagggcta tctgttcgaa ttgcgctgat gaaaaaatta
      181 ctgcatcttt ttttcccgct ctcgctgcgg gtacgttttc tgttagcaac ggcagcagta
      241 gtattggtgc tttcgcttgc ctacggaatg gttgcgctga tcggttatag cgtcagtttc
      301 gataaaacca cgtttcggct gttacgtggc gagagcaatc tgttctatac ccttgcgaag
      361 tgggaaaaca ataagttgca tgtcgagtta cccgaaaata tcgacaagca aagccccacg
      421 atgacactaa tttatgatga gaacgggcag cttttatggg cgcaacgtga cgtgccctgg
      481 ctgatgaaga tgatccagcc tgactggctg aaatcgaatg gttttcatga aattgaagcg
      541 gatgttaacg ataccagcct cttgctgagt ggagatcatt cgatacagca acagttgcag
      601 gaagtgcggg aagatgatga cgacgcggag atgacccact cggtggcggt aaacgtctac
      661 ccggcaacat cgcggatgcc aaagttaacc attgtggtgg tggataccat tccggtggag
      721 ctaaaaagtt cctatatggt ctggagctgg tttatctatg tgctctcagc caatctgctg
      781 ttagtgatcc cgctgctgtg ggtcgccgcc tggtggagtt tacgccccat cgaagccctg
      841 gcaaaagaag tccgcgaact ggaagaacat aaccgtgaat tgctcaatcc agccacaacg
      901 cgagaactga ccagtctggt acgaaacctg aaccgactgt taaaaagtga acgcgaacgt
      961 tacgacaaat atcgtacgac gctcaccgac ctgacccata gtctgaaaac gccactggcg
     1021 gtgctgcaaa gtacgctgcg ttctctgcgt agtgagaaga tgagcgtcag tgatgctgag
     1081 ccggtaatgc tggagcaaat cagccgcatt tcacagcaaa ttggctacta cctgcatcgt
     1141 gccagtatgc gcggcgggac attgcttagc cgcgagctgc atccggtcgc cccactgttg
     1201 gacaatctca cctcggcgct gaacaaagtg tatcaacgca aaggggtcaa tatctctctc
     1261 gatatttcgc cagagatcag ctttgttggt gagcagaacg attttgtcga ggtgatgggt
     1321 aatgtgctgg ataatgcctg taaatattgc ctcgaatttg tcgaaatttc tgcaaggcaa
     1381 accgacgagc atctctatat tgtggtcgag gatgatggcc ccggtattcc attaagcaag
     1441 cgagaggtca ttttcgaccg tggtcaacgg gttgatactt tacgccctgg gcaaggtgtg
     1501 gggctggcgg tagcccgcga aatcaccgag caatatgagg gtaaaatcgt cgccggagag
     1561 agcatgctgg gcggtgcgcg gatggaagtg atttttggtc gccagcattc tgcaccgaaa
     1621 gatgaataaa tatgtccata cttcacgcat tacgttaagc atccgttata atcggttgca
     1681 gataccagcc tgtggatgct taacatggaa taccaactca ctcttaactg gcccgatttt
     1741 cttgaacgtc actggcagaa acgcccggtg gtgttaaaac gcggctttaa taattttatt
     1801 gacccgatct ctccagacga gttggcgggt ctggcgatgg aaagcgaagt cgacagtcga
     1861 ctggtcagtc atcaggatgg caaatggcag gtcagccacg gtccattcga aagctacgat
     1921 catctcggtg aaactaactg gtcattgtta gtgcaagcag tgaaccactg gcatgagccg
     1981 accgccgcgc taatgcgacc gttccgtgaa ctaccggact ggcgtattga tgatctgatg
     2041 atctcttttt ctgtaccggg cggcggcgtc ggcccgcatc tcgatcagta cgacgtgttt
     2101 atcattcagg gtaccggtcg tcgtcgctgg cgagtgggcg aaaagctgca aatgaaacag
     2161 cactgcccgc acccggacct gttacaggtc gatccgttcg aagccatcat cgatgaagag
     2221 ctggagcctg gcgatattct ttatattccg ccaggattcc cgcatgaagg ctacgcgctg
     2281 gaaaatgcga tgaactattc cgtgggtttt cgcgcgccaa atacgcggga actgattagc
     2341 ggatttgccg attatgtgct gcaacgtgaa ctgggcggca actactacag cgatcctgat
     2401 gttccacctc gcgctcatcc tgcggacgtt ctgccgcaag aaatggataa actgcgtgag
     2461 atgatgctcg aattgatcaa ccagccggaa cactttaaac aatggtttgg cgagtttata
     2521 tcccagtcac gtcatgaact ggatatcgcg ccgccagagc cgccttatca gccggatgaa
     2581 atctacgatg cgctgaaaca aggtgatgtg ctggtgcgcc tggggggtct gcgcgtattg
     2641 cgcattggcg acgacgtgta tgccaatggt gagaagatcg attccccgca ccgtccggca
     2701 ctggatgcgc tcgccagcaa cattgcgctg accgcggaga attttggcga tgcgctggaa
     2761 gatccgtcat tcctcgcgat gctcgcggcg ctggtcaata gcggatactg gttcttcgaa
     2821 gggtaagttt ggatttaagc cggatgcggc atcacatgcc gcatcccttt tcgcttactt
     2881 ccgttgcgcc gtcaactcgg caatacggac gatcacctgc accgcttttt ccataccttc
     2941 cagagtcaca aactcatgct taccatgata gttgtaaccg ccagtgaaca ggttcgggca
     3001 cggtaatccc ataaacgaca actgcgcgcc gtcggtaccg ccgcggatcg gtttcagttc
     3061 cggttcaata tcgcagtcac gcatcgcctg ctgggcgata tcgagaatat gcggatgctc
     3121 aaccactttc tcgcgcatat tgtagtaact gtcttcaatc accagttcaa tgtagcaatc
     3181 aggatgtaac cctttgccca cttttttggc gatctccatc attttacgtt tacgcgcttc
     3241 aaactgttta cggtcgaaat cacggatgat gtagtgcata tcggcccgtt caacggtgcc
     3301 tttcatgctc gccaggtgat agaaaccttc atagccttct gtcatttccg ggctttcatc
     3361 cgccggaact tccgcatgaa tacgtgccgc cagcgacagc gcatttacca tcactccttt
     3421 cgccgtgccc gggtgaacat tgttaccgac aattttgata ttgaccgacg cggcgttgaa
     3481 gttttcaaac tccagttcgc cgacgccacc accatcaacg gtgtatgccc agcgggcatc
     3541 gaaggcgtca acatcaaaat gcttcgcccc tttgcccact tcttcatccg gggtaaaggc
     3601 gacgcgaata tcaccatgcg gaatgttttt ttgttgcaat accgccagcg cggtcatgat
     3661 ttctgcaata cctgctttgt catcggcacc taacaaggtt ttaccatcgg tggtaatcag
     3721 cgtctgaccc agtagctgat gcagcaccgg gaacataacc ggtgataaaa cttcatcgcc
     3781 gatacccagc gcaatatcgc caccgcgata gttttcaaca atttgcggat tcacattttt
     3841 gccgctgcaa tccggtgagg tatccacatg agaaataaag ccaatcgccg ggatatcgcc
     3901 agggacgtta gccggtaacg tcgccatcaa agtgcccttc tcacttaagg tcacattgat
     3961 aagccccatc tcttcgagct gctctttcag cagatgcaat aacttccatt ggccttccgt
     4021 gctgggaacc tgtctcaccc atgtcttcaa ccggcgtnnn nnnnnnnccc tgcctttgat
     4081 tgggtatcca gagacacgta gttcaaaaat cgctcaagta gtttatccat gtagtcaccc
     4141 tcactttttg tgacaacatt attaagaagc aagaaaagac aaatattgcg tcaggtcact
     4201 tttagccctg caagcgggaa tatttatcag cattacctta atgaatataa agctaagcca
     4261 gtaattcaca acaaagattg gcgattcaag cggtttgccg tagaattacc gccctcatta
     4321 agagtcacca aggtggttaa ccacaaaccc cgcatcggta agccatccgt tgcgtttaca
     4381 tgggacagag taaaaaattg aataaacaac cgagttcgct ttcaccgctg gtgcaattgg
     4441 cgggaattcg caaatgcttt gatggtaaag aggtcattcc ccagctggat ctgactatca
     4501 acaatggcga gttcctcacg ctgcttggcc cttctggctg cggtaaaaca accgttcttc
     4561 gcctgattgc aggtctggaa actgtt
//
LOCUS       DAWDRC010000315         4558 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8097_length_4558_cov_1.890740, whole genome shotgun sequence.
ACCESSION   DAWDRC010000315 DAWDRC010000000
VERSION     DAWDRC010000315.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8097_length_4558_cov_1.890740"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1717..1726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3976..3985
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1068 a         1211 c         1069 g         1190 t
ORIGIN      
        1 tggtaagcat tcgcacccgg attccatttc ttaaagaact ccgaaagatc cgtctgagcg
       61 acccaggagg cacacagcat cagcgtgtcg gcagcgttac cgttggattc agcacagtaa
      121 ttcttgccac caaaggtgct gttaccaaca tcatcgccgc gtgctttacg atgcatcaac
      181 tggaacaggt tccagccttt catcccttca cgctcgctgt agaacgcagg cagcttgcca
      241 tctggatacc acttcgtgat atcaaagttt ttctctgccc actctttcag ctgtgcgtac
      301 atcagcagac ggtcccccgc accgccgcgc gcccaggcct gaccgttgct ctcctccaga
      361 tattccggcg cgacggtaat gtcgtcagcg acacggttca tcttaccgag atagcgatcc
      421 tgcatgtaca gcgccagcac gttgttcgcc acttcagttg cacccggtac gttcagcggt
      481 gtttctgcag cgttatgacc gacttcgtgc caaatcagcc agtcgttcag cggcgtcgtc
      541 ggcagcgtgg tgctgttcgt cgagaagctg ctgttcatta ccggataacc agagtgcgca
      601 tcaccgatgg agatctgcac atcgttggcg aaacgatgtt tgtgacctgt caatgcttta
      661 taggtaaaca tccggtgctt accgtcttca tcattacgac cgtagaaatc attcatcgag
      721 ctggcaaagg tatccagatc tttagcgaat tgctccagtc cgcccgtgta attgctggca
      781 ttcaggttct tcttcggtgt ggtatagacg aacgacgcag actccagttc gcccagcggg
      841 gcaggagagt tcagatcgtt tttccatgcg ccgtctttat agaacggtgc ttttaccacg
      901 ccagtaaagg tgaagctggc agattcattg gtagagctat tgcccttgat ataaatcagg
      961 ccaccgtaag gcaccttgaa cttcaccgta ccattagcct ccagagtata cgttttagtc
     1021 actcttggcg gacggttcag cgcaacttca tgcttctcac gtccagtcag gtcgtcagcc
     1081 agcgccacgg taacagtcac aggaacgttc gccgtagact caatggtgac ctctttctga
     1141 gccggagccc acaggccagt tgattgcatg ttacctgcaa accatttggt cggattcgag
     1201 tacaggctga tggtttcagt aacgttctgt ccctcttccg atactgctcc cggatacttc
     1261 tcgacatcaa ctttgatgtt cagatcccac caggaacggc ccagcatcag acgcgtcagc
     1321 ggtttttcca tatagttgag cggatagctt gggttcatca tgcccgcttt gctgctaccg
     1381 tcaccgtaga tcatgttgtt atcgaccagc gattttttca gatctgcgga gcacttggtg
     1441 ccgcctgcat aggcatcatt ggcgtagcag ttcaggaact cggtgaacgt tttaaagccc
     1501 agctcatctt ccttgccctc ttcgtaacga tatttcgtat cgttccacag ccagaccgac
     1561 atgttctggt acagacgttc gagatcgacg ctgttcagac gttcgccttt actgccttta
     1621 gtacggaaat aaagctcatg ttgatacagg cgctgaatgt tggtgcctaa atctgcagcc
     1681 tgcaccatcg ctttcgcagt gtcggcgtca agattcnnnn nnnnnntata gcgtggaaca
     1741 aacatgccag tattattctc tggcgttaca ggaacccccg tgcctggacg gtattcgagg
     1801 cagttgacct catagtgata gtcaggatcc ttacactctt ccagcccagg gaacgcatcc
     1861 agaattctct tcttcgccgc agccagcaac tcaggcgttt tgtagtcggc ttcatcaata
     1921 aacgcgtatt gagttacttg ttcaccatca acctcttctt gccaactggc aacttccagc
     1981 ttcggtttct tatcaggttt gttatcaatc tgatatttcc aggtaacttc gcctgtctca
     2041 gaattaatgg tgtacggcag cgcaccttcc acaaccggat aacgttcata aacccaaatg
     2101 ccagtcgctc ggcgctggcg cacgcggttc ggataaccct gcggatcgtt gttcaccacc
     2161 gatttgttca gagccattga cagacccgcg gcatccagca gacgcacaaa actggacgcg
     2221 ctctcttcct taagattgct catcacgttt tccatgatca gcaccgaacc gcctttgttc
     2281 agataggcga tcagatcagt cacatcctgc tgagtcagct tcggtttgct ggtatctgca
     2341 cgcagaggca cagcataggg atcgccagac cactgagtca catattcaaa gccgttgagg
     2401 atcagcaacg gaacttcttc cggatttaga tcaccatagc tggtcaaatg tttaacagag
     2461 atacctgcaa aatccggatg gaagccaaat ggagcactgt tcccagtgac ctgaccatga
     2521 cgtttgaaat agacggtatc caggttggtg cctacggtca tgctggcttt cgcgtccggc
     2581 gtccatttat cgtcggacag atagcgcagt acgttctgca taaagtgctt catgtcatca
     2641 gaatcaccgc tgagcgtaca ctcaccttta ctattaacac caccgcccca actgtaacca
     2701 ttagggcaac gcaaaatgct gttgtagtgc gggttaccga taaccatcag tttgccgtcg
     2761 ccgacttgcc ccagcgaaat aaacggcagg ttgaaggtcg cggtatcgcg cgtaacgttc
     2821 tctggctgca caatggaagg cgcttccgta atgtacgcca gctcattttt atcccaggcg
     2881 cgtttttcac cgaaggccag ccagtagttt ttatcattac gcgccatcag aatcgggaat
     2941 gccgcgttgg agatattcac caccgcctga ccgcgcgcgt taccggtgct gccatagaag
     3001 ttggtagagt catggaagac gtggaacttg ctgacagact gatagttcgt atccacgccc
     3061 cacagcttgt tgataacgcc ctgaatctgg ccgtcattaa cattgcgcgt tgtcagcgag
     3121 aaccagcgag cctcgttgca accgttggtt ttcgcacaaa tcgcggtatc gatctctttg
     3181 gcctgacccg tcttaaactg ctcgataaat tcgttaggca gcacaacgtt ttgatcgcct
     3241 tcatccagcg tcgcaccgtt ggataacgaa agattgatta tctcgttgat cacgttggga
     3301 tattcggcaa agaccttgcg tacatcgtcc ggaacaacac gagtattatt ttgaccagtc
     3361 gtcgaataac gatgaatgag ctgatcgata tttgccccgc gaacttcatc acccaattca
     3421 gtcagcgcaa tggtcgactt attgccacgt actgagccca gttcaaaggt atcgatacca
     3481 aaggagatgg tttcgcccca gctaaaggag aatttaccgt tttcgtcagt tacgccacgg
     3541 cctgaattgg tgtagtagtc aacgccagca acaccgttcc ccaggctatc caccagttgg
     3601 ccttcggaaa gaatgatttc agtgggttga tactgataaa actgttccgc gttagccgac
     3661 acgaaggacg cgttcagatc cggttttgtt ccctctgtcg tgactggcac taccgttgag
     3721 gtatgggtgg acggcgcttt atcagtcgca gcattgtttt ccacctcttc attgaccagc
     3781 ttgctgaaat tgtctgttgc cagatcaatt tgcttataca gtttttcaaa tcgcgcgcga
     3841 tcaaccactg acgagaaagt aagacaaagc tgttctgcat ctgcggggca actgtcgctg
     3901 gacgtcacca gagagatggc gttggttttc ttattttcag aattcgccag ctcctgcgcg
     3961 tcctccaggc taaacnnnnn nnnnngcgta aaggtaaagc catcgctgga ttcaccatta
     4021 caggtagcac cagttacccg ctggcttccg cccagggtca gataacccgt tttcgtagga
     4081 acaggttctg gctccggttc tggtgtcggc tcaggatcag gtgttggatc cggcgtaggt
     4141 tctgggtccg gcgtcggctc aggtgtcggc tccggggttg gtgttggatc gggtttcact
     4201 tccggcaaag tccctgatcc agaatctaca gacggcgtat cggaggacga tcctgaacca
     4261 ccaccatcac aaccggctaa cagggttgcg cttaaaatag ccgctaaaag cgatttctta
     4321 tatttaaatt tcttattcat taataacgca agtgacaaaa caaagttaaa agattcgtaa
     4381 ccgaggaaat agttttatgc tttatcttct ttaataactt cctccattct taagaaaacg
     4441 gcatcattga tagaaaaaca ggtaaaattt ataagaataa cccctattcg atgtctggat
     4501 ggttattttt acgaaatttc aataaacaaa aaattaacaa ccattcacaa cgtaacta
//
LOCUS       DAWDRC010000316         4545 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8114_length_4545_cov_2.180178, whole genome shotgun sequence.
ACCESSION   DAWDRC010000316 DAWDRC010000000
VERSION     DAWDRC010000316.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8114_length_4545_cov_2.180178"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    306..315
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1079 a         1168 c         1113 g         1175 t
ORIGIN      
        1 cctttacccc gccgttggcg cggcaatatg caacttaatg gtatcgccag ggcgatagct
       61 ggctttatcc agtttcagcg tgacacggtc gggtcgcact gcaccacccc cgtcgctgtt
      121 atcctgccag ctataaccag cccagaaacg cacactactg accgcttcat tcggcgcttt
      181 aacttccaga cgataagcgc cccactctac cgggaaactg accttaccgg tttcgtccgc
      241 tttcagatcc agagtttgtt cattttcgat cagatctttt tgatcaaact gtgactgcca
      301 gccttnnnnn nnnnncctgc catgcaccgg cagcgcagat agcctgatca gaccggcgcg
      361 tcaccgggcg accgcccgat tccagcaaac taccctggaa aataacctgt aatggggaat
      421 gtgtttcctt ccactggctt tctgtagaaa cttcgccgcg ccctttatca tccagcgtca
      481 actgaacttc atccagcgtg cgggaaagat tttcggcagc tatatcgccg aattcaaaac
      541 caggtaaggc tgacacagct tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat
      601 taccattagc aggtgcgcca tacaggtagt accccaccac ggagaatttc acttcatctt
      661 tcggcgttag cggggttttc tcaccggtca gattcagcgc catacgctct ggcataaaat
      721 cttcgacgtg gaaatcccac atccgatact ggttatcgcc cgtgttagcg cgaatatgcc
      781 acataccggt tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg ccattctccg
      841 gctgactaac gacgctcctg agtacctgcc cgtcgggttt aatcacgtct aacttgatgg
      901 gttgatcggg caatgcttta ccgtctgcat cacgcagcaa accattgagg tagaccgttt
      961 cacccgggcg ataaagatcg cgcgggccaa acatgaaaaa ctgtttgcta tagcccggcg
     1021 cgccagcaat gttaaattcc gctaagtcca gcgccgggag ttttaaatcg agcagcgttg
     1081 tctgaccgtt tttacgcgcc agcagtaatg ccgcgttttt atcattttcc agctgcacat
     1141 gcccctgagc gtcactgctt gcctgagtca gagtctgccc tttctcattt aataaagaga
     1201 cttcaattcc ttgctgggcc gcgccgtttt ccagactttg ggtaaagata tccagacgat
     1261 tgtgataacg gtgagctgaa acgccaatat cacttaacgt aaacagcgtc gcgggattac
     1321 tgtaatcgta acgtccagcc tgattcatca cagccagata cacgcctgcc tgctgaagcg
     1381 gtttgatatc gcccagcggc agcaataatt tttcacgggt gttacgcgca ggattgagat
     1441 caaaccgtcc agtgtagacc agatccgcca tctgcagcag tttgtctgac tgccagttcg
     1501 ccagcgaatt gcggtattcc cattggctaa tgaaagcggg cagtgattct ggtttcacac
     1561 ggaagaagtt aacatcgaca ttgttaacgt tgagcgccat taccggcaac ccttcaataa
     1621 ctttaccagg taacaacgaa ccacggctgg caaaaccgac gctgggttgg atatcacggg
     1681 tagttatagt tttttcgtaa tctttactga aggttgcgtt gttgagtgct ttgacctcct
     1741 tgccaatagt gacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgc aactctttaa
     1801 gattatctga cagctcccag gcaccatcca ctttgccgct tttcttatcg acgacatgga
     1861 taacgcgtga gaaatcctga tccgggtcga gaggaatgga gaacgtcagc accagcgtgg
     1921 ctgcgccatc aagttgaact tcagagagat ccagcaatgt cagcgcctta ccggcactct
     1981 gttgggccag tttttgtcgc tccggcgcgg aaagttttgc tgaactcacg ttttctgaag
     2041 aggccgctgt agtaccttca gaaggcgcat cttttttcac cgctgttggc gcgttatcgt
     2101 tgttgtcgca ccctgccagc gccagcatta gcatgcaggc ggctacgcgt aactttttca
     2161 tttttcatcc ctgccacaat ggcccgttag caacgtcgaa taattattat gcgtgagaat
     2221 ttacgttatg taattcagta tcacgcccat acaatccgaa ttttagtgag aattgtggct
     2281 cagacagcca aatcagcctc ttttgcttgt cgcttttcac taaacatccg acaatttagc
     2341 ctaaccccgg caaaaatgga gatgcctatg tccacgacat ggtttgtagg agccgactgg
     2401 ctcgccgaac atattgatga cccggaaatt cagattatcg atgcccgcat ggcgtcgcct
     2461 ggacaggaag atcgtaacgt tgctcaggag tatatgaatg gacatattcc cggtgcagtg
     2521 ttttttgata tcgaagcgct ttctgatcac acttccccgc ttccgcacat gctgccgcgc
     2581 ccggaaacgt tcgccgtggc gatgcgtgaa ttaggcgtta accaggataa gcacctgatt
     2641 gtctatgacg aaggtaatct tttctcagcc ccacgagcat ggtggatgct gcgcaccttt
     2701 ggtgtagaga aagtgtcgat tctgggtggt ggacttgcag gctggcagcg tgatgatctg
     2761 ctattagaag aaggcgcagt agagctgccg gaaggagagt ttaacgccgt ggtaaaagga
     2821 cccactgaaa tactggcaag ccatgaaaat acggcgcaaa ttattgatgc ccgcccggct
     2881 acacgtttta acgcagaagt tgatgaacct cgcccaggtt tacgtcgcgg acatattccc
     2941 ggtgcattga acgttccgtg gacggaactg gtgcgcgaag gcgaactaaa aacgaccgat
     3001 gaactggatg cgatattttt tggtcgcggc gtcagctatg acaaaccgat tatcgtcagc
     3061 tgcggctctg gtgtaacagc agccggggtt ttgttggcac tcgcgacgct ggatgtgcca
     3121 aacgtgaaac tgtacgacgg cgcatggagt gaatggggcg cacgagcaga tttaccggtt
     3181 gagccagaga aataagcttt taacaggcaa taaaaaaccg ccgaatttgg cggtttttta
     3241 ttgctggtct ggttcgcggc ctttccagca gattggcttg tgttgcatga gcaacgcagg
     3301 tgcttcacag caaagcaata ctcgccagta actctctttt tgtcaagcga aagagagtaa
     3361 tcattgttta tttagcgtat tatcgacacc ggccctttcc gccgtctcgc aaacgggcgc
     3421 tggcttaagg aaaggatgtt ccatggccgt aaatgcaggt gtttcacagc gcttgctatc
     3481 gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc gcatggaccg cacaatgaag
     3541 atacggtgct tttgtatcgt acttattgtt tctggtgcgc tgttaaccga ggtaaataat
     3601 aaccggagtc tctccggcaa caatttactg gtggttaaca accttcagaa cagcaagtaa
     3661 gcccgaatgc cgccctttgg ggcggcatgt tttaaattat ccggttctgt ttaaagtcac
     3721 gcaaaaaacc accccagcga cgttcataga atggcgcaat atgttcggta ataaagtggc
     3781 taattccttt ttcccctttt ttcacctgac aaatatcgat gggttcatcg ccaggtaatg
     3841 tatcggtcgc tacacttccc gccgcctgaa taatttcttc gatatcacca tcggcttcaa
     3901 tgccaataag taaattaggc tgtgcctctt cgttctcttt aattgaacaa ataaaagcac
     3961 gcttcaccgg cttaatggtt ttaaataagg tggtaagtga atcaatcatt tgcgctggcg
     4021 gctctgcgac ctccgataat atcagcgatt caccgccttc cagtacttcc tgactgctaa
     4081 gcggattccc ttcttcacca atcagcaaac tgatttcacg cggcataaat tctttaccgg
     4141 ttggcagttt ggcattaagg aagagcgttt cgccaagggt catctcaaac aatgtgcgaa
     4201 ctggcattac gacaaatgcc tgttcgcctt caaccgcctg ttgaagtgct tctaacgagg
     4261 tgaaaaaagg aatgacgctg gtgccgtctt ctttttccca gtgctgtaaa tcaagtgcgc
     4321 tatcttcaac cacagcctcg ccctgtgccg ccgtaccagg cacccagacg gtggattcca
     4381 gtagagtacg gaaaaaggcc gggcggtgcg ccggttcggt tgctgctttt tccagcaggt
     4441 cttcaagttc gttttttgtt tcggacataa gaaccataat tcattcaacg ttcggggcgt
     4501 aagtgcctga tgcgctacgc ttatcaggcc tacaagggaa ccgca
//
LOCUS       DAWDRC010000317         4533 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8139_length_4533_cov_2.451318, whole genome shotgun sequence.
ACCESSION   DAWDRC010000317 DAWDRC010000000
VERSION     DAWDRC010000317.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8139_length_4533_cov_2.451318"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2305..2314
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1163 a         1230 c         1048 g         1082 t
ORIGIN      
        1 gccttcttca ccgcgctctc tccagacgca caggacgata tcatcgcccg taccgaacac
       61 atgctgtaat cccctcagcc cggcgtcgtc gtcgccgggc caaatcattc acatcatcaa
      121 tttcagcctc gctttcattc gaaatataat ttgtgctctg cgtcacattg ttattacact
      181 gttacggttt cattttcgag ccaggagcac acctatgagc gtaaaagtta tcgtcacaga
      241 catggacggt acttttctta acgacgccaa aacgtacaac caaccacgtt ttatggcgca
      301 atatcaggaa ctgaaaaagc gcggcattga gttcgttgtc gccagcggta atcagtatta
      361 ccagcttatt tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg
      421 cgcactggtt tacgaacatg gcaagcagtt gttccacggc gaactgaccc gacatgaatc
      481 gcggattgtt attggcgagt tgctaaaaga taagcaactc aattttgtcg cctgcggtct
      541 gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattc gtcgcactga tggcaaaaca
      601 ctaccatcgc ctgaaacctg taaaagatta tcaggagatt gacgacgtac tgttcaagtt
      661 ttcgctcaac ctgccggatg aacaaatccc gttagtgatc gacaaactgc acataacgct
      721 ggatggcatt atgaaacccg tcaccagtgg ttttggcttt atcgacttga ttattcccgg
      781 tctacataaa gcaaacggta tttcgcggtt actgaaacgc tgggatctgt caccgcaaaa
      841 tgtggtagcg attggcgaca gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc
      901 ctttgcgatg agcaatgctg cggaaaacat taaacaaatc gcccgttacg ctaccgatga
      961 taataatcat gaaggcgcgc tgaatgtgat tcagacggtg ctggaaaata aaccgccgtt
     1021 taatctttga ttatcgccaa ttcagtttat tgagaaagtg catgtcatta tgccggatac
     1081 ggcgtaaccg ccttatttgg catacaaaat catgaaaatt caatatattg cgatacactg
     1141 ccaggtctga taagcgtagc gcaccaggca atcttgcgtt tgtcatcagt ttcagtagcc
     1201 ctgaccatat cagggctact gataataact tactcgatcc ctttactacg caggtaatct
     1261 tcgtaattac cgctaaagtc gatcacgcgt tccggggtga tttccagaat acgggtcgcc
     1321 agggagctta caaactcacg gtcgtgagaa acaaagatca gcgtgccctg atacagttcc
     1381 agcgccatgt tcagcgactc aatggattcc atatccaggt ggttggtcgg ttcgtccatg
     1441 atcagaatgt tcggcttctg catcatcaac ttaccaaaca gcatccgccc tttttcccca
     1501 ccggaaagca ctttagctgg ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga
     1561 atgctgcgta ccgcctgctc gtcatcgcct tcctgcttcc actgactcat ccattcgaac
     1621 acggtcagat cattttcgaa ctcatattca tggtcctggg cgtagtaacc aatgcgcgcg
     1681 ttctcagacc attttacggt gccgctgtcc ggttgcagat cgcccaccag cgttttcagc
     1741 agcgttgatt taccgacgcc gttggtaccc agtaccgcca gtttttcacc cacttccagc
     1801 agcaggttga gatttttaaa cagtggaccg ttatcaaacc ctttagtcag accttccact
     1861 tccagcgcgt tacggaacag tttcttatcc tgttcaaaac ggatgaacgg gttctgacgg
     1921 ctggaagctt tcacctcttc cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc
     1981 tggcgagatt tcgaggcgtt ggcgctaaag cggctaacga aagattgcaa ctcagcaatc
     2041 tgcgctttct tcttggcgtt atcagccagc agacgttcac gcgcctgagt cgccgccgtc
     2101 atgtactcat cgtagttgcc cggataaacg cgcagctcgc cgtaatccag atccgccatg
     2161 tgggtacaaa ccatgttaag gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca
     2221 cgctcgttca gcacctgttc cagccagcga atggtgtcga tgtcgaggtt gttggtcggt
     2281 tcgtcgagca ggagaatgtc cggannnnnn nnnnccgtat ttcacttcca gatcggccac
     2341 tttatagccg tcttcttcac tcatttccgg caaagcatag atgcggtcac gctcctgctt
     2401 cacttcccac aactctttat gccccatgat caccgtatcc agcacagtga actcttcaaa
     2461 ggcaaactga tcctgacgca gcttaccaat gcgctcgttg ggatcgaggg aaacgttacc
     2521 caacgtcggc tctaagtcgc cgccgaggat cttcataaag gtggatttac cactaccgtt
     2581 cgcgccaatc aggccgtaac ggttgccgcc gccgaatttg acggaaatgt tttcaaacaa
     2641 cggcttactg ccgaactgca tggtgacgtt actggaaact aacacaggcg tatcctgaaa
     2701 agagatatga caaaccgcgt attatgccat aagtctgaaa gaagatcctg ctcctcgctt
     2761 aaggtctaca ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg atttcactgt
     2821 gagctggaat gaacttataa tgcgcttcca gtactctaat attctcaacc caatggcctg
     2881 ccaggcacaa aatctcgctt aacatgaata tgaaattgaa aacattattc gcagcggcct
     2941 tcgctgttgt cggcttttgc agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg
     3001 ggagtcgcct ggttggtcag aatcaggtga tcaccattcc tgaaggtaac actcagccgc
     3061 tggagtattt tgccgccgag taccagatgg gactttccaa tatgatggaa gcgaacccgg
     3121 gtgtggatac cttcctgccg aaaggcggta ctgtactgaa cattccgcag cagctgatcc
     3181 tgccggatac cgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt ctgtattact
     3241 atccgaaagg gaccaacacc gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag
     3301 acacgcctat caactggacc accaaagttg agcgtaaaaa agcaggcccg acctggacgc
     3361 cgaccgctaa aatgcacgca gagtaccgcg ctgcgggcga accgcttccg gcggtcgttc
     3421 cggctgctcc ggataacccg atggggctgt atgcactcta catcggtcgc ctgtacgcta
     3481 tccatggcac caacgccaac ttcggtatcg gcctgcgtgt aagtcatggt tgtgtgcgtc
     3541 tgcgtaacga agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc cgcgtacagt
     3601 ttattgatga gccggtaaaa gcgaccaccg agccagacgg cagccgttat attgaagtcc
     3661 ataacccgct gtctaccacc gaagcccagt ttgaaggtca ggaaattgtg ccaattaccc
     3721 tgacgaagag cgtgcagaca gtgaccggtc agccagatgt tgaccaggtt gttctggatg
     3781 aagcgatcaa aaatcgttcc gggatgccgg ttcgtctgaa ttaatcttca aaacgtaaag
     3841 caaaggcgga ctcataatcc gcctttttaa tttgacagac cttatcccgc cgggatcata
     3901 accaacaaaa catatcccac cagcgccgcc cacaacagca tcggtatcga ataaagatga
     3961 aaacgccacc agatacggcg atcgttcgcc atccgtaacg caatcaaatt tgccagcgat
     4021 ccgggtaata aaccaaagcc acctacgttt accgcccatg ccagtaataa agacggtggc
     4081 acataattga gcagcaaaat ggtactcggc acgttactga tcacctgcga taaaccaatc
     4141 gccgttaacc ataatccggg gtcggataaa tgactcacgt tacttaacac cccctgcaaa
     4201 gctggcagtt gggttagtaa atggacgtcg ataaacatcg ccataaacac cagcagcagc
     4261 gtccagtcca cactgagcac cacgcgacgc gccagcagcg caaagcccgc cgccacaatc
     4321 accagtcccc acagctcttg ttttaactcc agcgccgtca gaaagacgat atacagcccc
     4381 agacaactcc aaaccagccg cggtttccac tccggcgttt gcccccccgt atggtattgc
     4441 agcgcctttc cagggaaaca acaccagcac aacagcagga gtgtcagcat cattgcgcca
     4501 gccagcggtg ccatttgggc aataaatccg gca
//
LOCUS       DAWDRC010000318         4532 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8142_length_4532_cov_2.011615, whole genome shotgun sequence.
ACCESSION   DAWDRC010000318 DAWDRC010000000
VERSION     DAWDRC010000318.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8142_length_4532_cov_2.011615"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2069..2078
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2568..2577
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4035..4044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          958 a         1179 c         1231 g         1134 t
ORIGIN      
        1 gaacagtaac cgtccgatgg actgggactg gattaaagcg ttcccgcaaa cgctgaaaga
       61 tgaatttaag tcgatgaaaa tcacggtgaa ctgggaaaaa gcctggcccg ccgtattcat
      121 cgccttcctc gctggtttgc cgctgctgtt gattgccggg ctgattcact ggcgtttagg
      181 ctggctgaaa gcatatcaac aaaaactggc ttcggcggta ggttccctgc gtaacgatag
      241 ccagctcaat acgccaaaag cgattcttat tgacctaatc cgcgcactgc cggtgtgcct
      301 gattattctc gctgtcggcc tgattctgct gactatgcag ctcaatatca gcgagttatt
      361 gtggtcgttc agcaaaaaac tggcgatttt ctggctggtg tttggcctgt gctggaaagt
      421 gctggagaaa aacggtgttg ccgtgcgtca ctttggtatg ccggaacagc agaccagcca
      481 ctggcgtcgg caaattgtcc gcatcagcct cgcgttgctg ccgatccatt tctggtctgt
      541 ggtggcagaa ctctccccgc tgcatctgat ggatgatgtg ctggggcagg cgatgatttt
      601 cttcaacctg ctgctgattg ccttcctggt gtggccgatg tgccgcgaaa gctggcgtga
      661 taaagagtcg cacaccatgc gactggtcac cattaccgtg ctgtcgatta tcccgattgc
      721 gttgatggtg ctgaccgcca cgggttactt ctacaccacg ctgcgcctct ccgggcgttg
      781 gattgaaacc gtttatttgg tgatcatctg gaacctgctg taccagacgg tattgcgcgg
      841 cttaagcgta gcggcgcggc gtatcgcctg gcgtcgtgca ctggcgcgtc ggcagaatct
      901 ggtgaaagag ggcgcagaag gtgccgaacc gccggaagaa cccaccattg cgctggagca
      961 ggttaaccag cagacgctgc gtattaccat gttgctgatg tttgcgctgt tcggtgtcat
     1021 gttctgggca atttggtctg atttgatcac cgtgttcagc tatctcgaca gcattacgct
     1081 ctggcattac aacgctactg aagctggtgc tgcggtggtg aaaaacgtca ccatgggcag
     1141 tctgttgttt gcgattatcg cctcaatggt ggcctgggcg ttgattcgca acctgcctgg
     1201 tttgctggaa gtgctggtgc tctcgcgact gaatatgcgc cagggcgcgt cgtatgccat
     1261 tactactatc cttaactaca tcattattgc cgttggtgcg atgacggtgt tcggatcgct
     1321 gggcgtctcg tgggataaac tccagtggct ggcggcagca ttatccgtag gtcttggttt
     1381 tggtttacaa gagatcttcg gtaacttcgt ttccggtttg atcattctgt tcgaacgtcc
     1441 ggtgcgcata ggcgacacgg taaccattgg tagcttctcg ggtacggtca gcaagatccg
     1501 tattcgtgcg acaacgatta ccgatttcga tcgcaaagaa gtgattatcc cgaacaaagc
     1561 gtttgttacc gagcgtctga tcaactggtc gttgactgac acgactacgc gtctggtgat
     1621 ccgtctcggc gtagcctatg gctccgatct ggaaaaagtg cgtaaagtgt tgctgaaggc
     1681 ggcgactgag cacccaaggg tgatgcacga accaatgccg gaagtcttct ttacggcatt
     1741 tggtgccagc acgttggatc atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag
     1801 ccgtactgtc gatgagctga accgtactat cgatcagctg tgccgtgaaa acgacatcaa
     1861 cattgccttt aaccagcttg aagtgcatct gcacaacgag aagggcgatg aggtgacgga
     1921 agtcaaacgc gattacaaag gcgacgatcc gacaccagcg gttgggtaat aaccaaaggg
     1981 ggcgacattt agttgccccg agattactaa caaagtgcgc gttgttcatg ccggatgcgg
     2041 cgtgaccgcc ttatccggcc tacgaaacnn nnnnnnnnga taagcgtagc gcatcaggca
     2101 gttttgcgtt tgcccacaat cttaggggac atttagcggc cccatttatt tctcactttt
     2161 ccgcctcatc atcgcgcgtt aatttctttt cataatcgcg ttttacaata tccagcgcgc
     2221 gcagcacggt gcgggctggg atctgatttt cttccagcag cacaatcaaa tcgacggcca
     2281 gtttgacatc gtcaggggca ttttccagtg acatactctc tccattgctg gcgggttaaa
     2341 cgcgctaacc tgttttcgat tttttccagc gcatggcggc agcgtgccag gcgcgcttca
     2401 taggcttcca cttcacgatg cagcgtctgc tgttccacga gatcggtcgc tcgcgccaga
     2461 cgggctctac gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata
     2521 cgtttacgct gccagcgggc aattttcggt ggtgcgctat cccactcnnn nnnnnnncgt
     2581 agcgaccagg ctgtggcttc acgcgctata gcctccagtt gcgccgccag atgttctgcc
     2641 agccaggcga cttgcggcag ctgttgctgc tcaaccgcat gacgaagcgc agccaggtta
     2701 tcgcccgcct catcgagaca agcctgtagt gttgtcgcac gggtctgaaa aaggtgcctg
     2761 tcaaaacggg cacttagcgt ggcaaattgc gctaccgggg cacaacgctg acgcagcgtg
     2821 gcgagctgtc cttccagttt ttccagcagc agggcggttt tcacgataag acctcaatga
     2881 aaatgataat tgttatgcta aagtagccac tctgtaagct gacaacactc aatatgcaac
     2941 gaatcatttt aatcatcatt ggctggctgg cggtagtgct gggtacgctg ggcgtggtat
     3001 taccggtatt accgacgacg ccgtttatcc tgctggcggc ctggtgcttt gcccgttctt
     3061 ccccgcgctt tcacgcctgg ttgctgtacc gctcatggtt tggcagctat ctacgtttct
     3121 ggcagaaaca tcatgcgatg ccgcgcggcg caaaaccacg ggcgattttg cttattttga
     3181 ttacgtttac gatttcgctg tggttcgtcc agatgccgtg ggtgcgcatc atgttgctgg
     3241 taattctcgc ctgtttgctt ttttatatgt ggcgaattcc ggtgattgat gaaaagcaag
     3301 aaaagcactg aagcacaaca atcgcagttg caattattgc gtacagccag tacattctgg
     3361 cgttttcgag cacaggcgca ggcggtcaaa ggttaaacaa ctgttacttt tgatacgttc
     3421 aaaacgcgcc gtgagtacca ccgtaacaag caggcataca cttatgaccg cgactgcaca
     3481 gcagcttgag tatctcaaaa atagcatcaa aagcattcag gactacccaa aacccggcat
     3541 tcttttccgc gatgtcacca gcttactgga agacccgaaa gcttacgctc tcagcatcga
     3601 cttgctggtt gagcgttaca aaaatgcggg cattaccaaa gtggtcggca ccgaagcgcg
     3661 tggcttcttg tttggcgctc cggtagctct gggtctgggc gttggctttg taccggtccg
     3721 taaaccgggc aaactgccgc gtgaaaccat cagtgaaact tacgacctgg aatacggcac
     3781 cgatcagctg gaaatccacg ttgatgccat caaaccgggc gataaagttc tggtggtgga
     3841 cgatctgctg gcaaccggcg gcactatcga agcgaccgtt aaactgatcc gtcgtctggg
     3901 tggtgaagta gctgacgctg cgttcattat caacctgttc gatctcggcg gcgaacagcg
     3961 tctcgaaaaa cagggcatta ccagctacag ccttgtcccg ttcccgggcc attaattatc
     4021 gtcagtcttg cgctnnnnnn nnnnacgcta cgggcagggc aagatgtgca ttcagcctcg
     4081 ccgttctgac ggggctgtgt tagcattacc ccttcgtgaa tccaccttcc agcgtttcag
     4141 agcctgccaa tgagttatca ggtcttagcc cgaaaatggc gcccacaaac ctttgctgac
     4201 gtcgtcggcc aggaacatgt gctgaccgca ctggcgaacg gcttgtcgtt agggcgtatt
     4261 catcatgctt atcttttttc cggcacccgt ggcgtcggaa aaacctctat cgcccgactg
     4321 ttggcgaagg ggctaaactg cgaaaccggc attaccgcga cgccgtgcgg cgtgtgcgat
     4381 aactgtcgtg aaatcgagca ggggcgcttt gtcgatctga ttgaaatcga cgccgcctcg
     4441 cgtaccaaag ttgaagatac ccgcgacctg ctggataacg ttcagtacgc tccggcgcgt
     4501 ggtcgtttca aagtttatct catcgacgaa gt
//
LOCUS       DAWDRC010000319         4523 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8159_length_4523_cov_2.089302, whole genome shotgun sequence.
ACCESSION   DAWDRC010000319 DAWDRC010000000
VERSION     DAWDRC010000319.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8159_length_4523_cov_2.089302"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2575..2584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    4135..4144
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1032 a         1230 c         1117 g         1124 t
ORIGIN      
        1 tcgtcgttgt tgtcaaagga acccacgtta cgcggattct cgtaatggtc gataactttt
       61 tcgctgtaag ccattataaa ttctcctgat tccgataccg attaatgatg agcccattcg
      121 atgctgttca gatccacgcc ctgcttgtac atttcccaca gcggagaaag gtcacgcaga
      181 cgaccgatgg atttacgaac taactcgatg gtgtagtcga tctcttcttc agtagtaaaa
      241 cgacctaaag agaaacggat agagctatgt gccagctcgt cgttcagccc cagcgcgcgc
      301 agcacgtagg acggttcgag gcttgctgac gtacaggcgg aacctgaaga aaccgcgagg
      361 tctttcagcg ccataatcag cgactcacct tcaacgtagt tgaagctgac gttgagaatg
      421 ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt cttcgatatc tttgatgccg
      481 ttccacagac gattacgcag gccgcgcaga cgttccatct cggtcgccat ctcttctttt
      541 gcgatgcgat aggcttcgcc cataccgacg atctggtgaa caggcagagt gccggaacgc
      601 ataccgcgct catgaccgcc gccgtgcatt tgcgcttcga tgcgtacacg cggtttacga
      661 cgcacataca gcgcaccaat acctttcggg ccatagattt tgtgaccgga gaaagacatc
      721 aggtcaactt tcaactggct cagatcgata ggcagtttgc ccacgctctg ggttgcatca
      781 acgtgataga taatgccacg agcacggcac atttcgccga tagccgcgat atcctgcacc
      841 acgccgattt cgttatttac gtgcatgatg gaaacaagga tggtgtcatc acgcatcgct
      901 gcttccagtt ctttcaggtc gataatgccg ttacgctgcg gtgccaggta agtgacttca
      961 aaaccttcgc gctccagctg acggcaggta tccagtaccg ctttatgttc ggttttactg
     1021 gtgatgatgt gcttgccttt tttctgataa aagttggctg cacctttgat cgccaggttg
     1081 tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg gatcagcgcc gaccagatcg
     1141 gcaatctgat tacgggcgat atctaccgct tcttcagcct gccagccgaa acggtgagaa
     1201 cgggaggccg ggttaccaaa ggttccgtcc atcgtcataa actgcatcat tttctcggca
     1261 acacgcgggt ccaccggcgt ggttgcggag tagtcgagat aaatcggtaa tttcattgct
     1321 ctataaactc cgtacatcac tcaatgcaag gaatcaggct accggctgga tgtacgaccg
     1381 tgtttacgga gtatttagca ctccggcctg attctgaatt ctttttatta agcgcgtaac
     1441 ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca
     1501 gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt attgagaaaa
     1561 ccggtaagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt atcgccgccc
     1621 tggcagccgc ctttaccctg acaacgggtg gcatctacag attcgtcaac ggcgctaatc
     1681 acctcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc gcctggtcca
     1741 cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc cagataagaa
     1801 agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc ttcagagttg
     1861 agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag tctcatgtct
     1921 tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg gggtattgca
     1981 tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt gactgattta
     2041 gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct gttgtcggat
     2101 gcggtgtgaa caccttatcc aacctgagat tccgtgcact tacaggcctg ataagacgcg
     2161 ttaagcatcg catcaggcaa ccactgttat tcggccttgt tacctttatt ctgctgctca
     2221 atagaagcca gaatcccgcg caggatattc aattcctggc tttccgggcg tgcacgggta
     2281 aacagacggc gcaatttatt catcacctgc cccggatggt tttcacggat aaaaccggtt
     2341 gccagcagcg tttgctccag atgaccataa aaacgctcca gatcatcgac cagcggatac
     2401 ggcgtctctt catgctcgac ctgctcgcca ttttcctgag ttgccagcca ggccatacgc
     2461 acttcatagg caataacctg aaccgccatc gccaggttca gcgagctgta ttccgggttc
     2521 gcggcaatcg cgacatgata atggcatttc tgcaactctt cattggtcaa gccannnnnn
     2581 nnnnagccaa cacgctcacg accaaaaacc agcgccaccg gggtatttgc cgcttcagcg
     2641 acgcttttca gaccgcattc gcgcgggtcg agcatcggcc acggcagcgt gcgggagcga
     2701 gcgctggtgc ccaccaccag actacaaccg gctaacgctt cgtcgagcgt atcgacgatg
     2761 tgagcattac caatcacatc gctggcccct gctgccaggg caatcgcctg ggagtcaggt
     2821 ttcactagtg gattaaccag ccacagattg gttaatccca ttgttttcat ggcacgggca
     2881 acagaaccca tattgccggt gtgtgacgtc tccaccagca caattcgaat attttgcagc
     2941 attgtctttc ttcatctaaa gattattcac gcatcttatc ataaaacgaa gacagatgcc
     3001 gatctcgctg ctatactctg cgccgttttc ccgttcttta acatccagtg agagagaccg
     3061 atgcatccga tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc
     3121 aaaaactatg aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg
     3181 accaacgtag ataaagctgc cgaagcggtg attatcgaca cgattcgtaa atcttaccca
     3241 cagcacacca tcatcaccga agaaagcggt gaacttgaag gtaccgatca ggatgttcaa
     3301 tgggttatcg atccactgga tggcactacc aactttatca aacgtctgcc gcacttcgcg
     3361 gtatctatcg ccgttcgtat caaaggccgc accgaagttg ctgtggtata cgatcctatg
     3421 cgtaacgaac tgttcaccgc cactcgcggt cagggcgcac agctgaacgg ctaccgtctg
     3481 cgcggcagca ccgctcgcga tctcgacggt actattctgg cgaccggctt cccgttcaaa
     3541 gcaaaacagt acgccactac ctacatcaac atcgtcggca aactgttcaa cgaatgtgca
     3601 gacttccgtc gtaccggttc tgcggcgctg gatctggctt acgttgctgc gggccgtgtt
     3661 gacggtttct ttgaaatcgg tctgcgcccg tgggatttcg cggcaggcga gctgctggtt
     3721 cgtgaagcgg gcggcatcgt cagcgacttc accggtggtc ataactacat gctgaccggt
     3781 aacatcgttg ctggtaaccc gcgcgttgtt aaagccatgc tggcgaacat gcgtgacgag
     3841 ttaagcgacg ccctgaagcg ttaatgactc tggcgggtga tgtcactcac ccgccctcgc
     3901 ctttcaggcg ctattccgaa atacttcctc accgctttac tttctttcgc cactctccca
     3961 ccatctttcc ccgattaatg gatgataaag aagtcgcaag gaaaggttat ggcgctgcca
     4021 gtgaacaaac gcgttctcaa aattctgttt attctctttg ttgttgcctt ctgcgtttat
     4081 ttagtgccgc gcgtcgccat caacttcttc tattatcctg acgataaaat atacnnnnnn
     4141 nnnngtcccg acccctggtc ggcggaatcc gtcgaattta cggctaagga cggtactcgt
     4201 ctgcaaggct ggtttattcc ttcttcgacg ggccctgctg ataacgccat tgctaccatt
     4261 attcatgctc acggcaatgc cggaaatatg tccgcccact ggccgctggt cagttggtta
     4321 cccgagcgta atttcaacgt ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc
     4381 acgccgtccc aggccggatt gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat
     4441 cgcagtgatg taaacccaca acggctggtg ctgttcgggc agagtattgg cggggcgaat
     4501 attccggatt ttattggtca ggg
//
LOCUS       DAWDRC010000320         4509 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8176_length_4509_cov_2.061293, whole genome shotgun sequence.
ACCESSION   DAWDRC010000320 DAWDRC010000000
VERSION     DAWDRC010000320.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8176_length_4509_cov_2.061293"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1594..1603
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2290..2299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3813..3822
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1134 a         1222 c         1020 g         1103 t
ORIGIN      
        1 ctgaagcaac atctttggct gctttctttt tttaatattg ctggccgatt cattttctga
       61 tatcggccat ttttattgcg ggtatcaggc cagtttatgg ttagccatta attccagcgc
      121 ctgcactaat gcagagtgat ccaactggct gccaccgttt gccgcacagg tattaaacaa
      181 ttcctggcag gtcgcggtgt ttggcaggtt cagcgccagt gctttcgcac tttgcagtgc
      241 caggttgaga tctttctggt gcagcgcaat tttgaaacct ggattaaagg tgcgtttaat
      301 catgcgctca ccgtgaactt ccagaatccg tgaggaagca aagccgccca tcagcgcctg
      361 gcgcacacgt accgggtccg caccggcttt tgaagcaaac aacaaggctt cagaaaccgc
      421 ttcaatattg agcgccacga taatctgatt tgccactttg caggtttgac cgtcgccgtt
      481 accgcccacg agggtgatat ttttaccgag cagttcaaac agcggtttta cacgttcaaa
      541 tactgcttca tcaccgccaa ccataatcga taacgtccct tcacgcgcgc cgatttcacc
      601 gccggagact ggcgcatcga gataatcgcc gcccagttca ttcacctgac gagcgaaacg
      661 tttagtttca atcggggaaa tggagctcat atcaacaatg gttttgccct tcagcgaggc
      721 tttggtacaa ccattttcac caaacagaac gtcttcaacc tgtggcgtat ccggcaccat
      781 aataaaaatg atgtccgatg catctgttac ctggcgcgca gtttcaacac taacggcgcc
      841 cagtgacagt aattcatcag caaccggacc aatggttgtg acatgcagtt gatgaccggc
      901 acgcgccaga ttaatggcca tcggtgtacc cataatgcct aagccaataa atcccagttt
      961 cataattaac ctcttttaaa tttcgctttt cctgaattca gacaacacga tcccctgcca
     1021 acttagcggc ctaaaaaagc attgtctgaa tagcgttacg ttttaacggt acgggtccat
     1081 ccagcgtaaa ccggcttccg tggtggtttg gggtttatat tcacagccaa cccaaccgtt
     1141 gtagtcagaa ttttcaatta ccgtaaagag atattcgtaa ttaatttctc cggttcccgg
     1201 ttcgccgcga tgcggattat cagcaatttg caggtgacca attttatcgg cccactgagt
     1261 catggtatta gttaattcac cttccatccg ctgcatgtga taaatgtcat actgaatttt
     1321 caaattgcag caaccaacat cgtcaatcag tttcagtgcc tgccgggttc cggtgagatg
     1381 gaaaccagga atatcaaaat ggttaatagg ttcaatcagt aataaaatat cttctttcat
     1441 cagcatattc gcagcataac gcaggttttc tacaagcgtt gtgtgaatct gttcactgct
     1501 gaaaccagtc ggcgttttac cgaccagaca gttaattttt ttattaccca gagcacgagc
     1561 ataacgaatc gctgctgcta cgccatcccg aaannnnnnn nnncgcccag tcacctgccg
     1621 gtaaattgtg cagcgtatgt tcgagtttat tactcgccag cacctgtttt aattcttcaa
     1681 tgtcgtagtc ataagggaac ataaattcaa cgccgcgaaa accacactgc gcagctttct
     1741 caaaacgggc gagaaaatca tattcaccaa ataacatcga taaattagca gagaaacgta
     1801 acattattta tctcccttat tcatagtgca tgaaacaggt ttcagtcggt gcatccgcgg
     1861 cgttatcggt gatatcttca aattccatga cgttatccag ttcactgccc atcgaaatat
     1921 tggtcacacg ctcgagaata acttccacga ctaccggtac ccgatattgc gccattaaga
     1981 cttttgcctg ttcaaaggct ggtgcaatat cttccggttt gaaaacgcga attgctttac
     2041 aacctaaacc ttccgctact tttacgtggt cgacgccata accgttcact tcgctggagt
     2101 taatattttc gaacgcgagc tgtacgcagt aatccatatc aaaggcacgt tgcgactggc
     2161 gaatcagacc cagataggcg ttattgacca gtacatggat gtacggaatg ttgaactgtg
     2221 cgccaacggc taattcttca atcaggaact ggaagtcgaa gtcaccggaa atcgccacca
     2281 cattgcgctn nnnnnnnnna gtgccgcagg aatcgtccat cccaaaggac ccgcctgccc
     2341 acagttgatc cagtggcggt ctttaaagac atgcagcatt tgtgccgcag cgatttgtga
     2401 caggccaatg gtggtgacat aacaaacatc gcggccaaag gctttgttca tctcttcata
     2461 cacgcgctgc ggtttcaccg gcacgttgtc gaagtgggtt ttgcgcagca aagtgcgttt
     2521 gcgctgctgg cagtcggcga cccattcttt acgacacggc agacgaccgg ctttttgcat
     2581 ctcctgcgcc acttcaacca gcagcgtcag cgccgcttta gcatcagaga caatgccaag
     2641 atccggacac agtacgcggc caatttgcgt cggctcaata tcgatatgaa cgattttgcg
     2701 cccttcggtg tatttctcta ccgaaccggt atgacggtta gcaaaacggt taccgatacc
     2761 aaacaccatg tcggacgcca gcaacgttgc attaccgtaa cgatgcgcgg tttgcaggcc
     2821 caccatcccg gccatcagtt catgatcgtc cgggatacag ccccagccca tcagcgttgg
     2881 gatcaccgga acgctggtca gttcagcaaa ctgttgtaac agtgcagctg cgtcagcatt
     2941 gattaccccg cctccggcaa caatcaccgg acgttcggcc tggattaaca tttctacagc
     3001 tttttcgatc tgcatacggc tggcagcagg tttgtagacc ggcagcggtt cgtacatgtc
     3061 aggatcaaac tcgatttccg caacctgtac gtcgaacggt aaatccacca gtaccggacc
     3121 cgggcgacca gaacgcatca ggtgaaacgc ctgttgcagc acacgaggca ccagcgccgc
     3181 ttcacgaact gtaaccgcca ttttgctgac cggtttagca attgcttcaa tatcaacggc
     3241 ctggaaatct tctttatgca gacgggcgcg cggagcctgg ccggtaatac acagaatagg
     3301 aatggaatca gcagaagcgg aatagagcgc ggtgatcatg tccgtgcccg caggaccgga
     3361 agtccccaga catacgccga tattccctgc cgttgcgcgg gtataacctt ccgccatgtg
     3421 cgaagcacct tccacatgac gcgccagaat gtgacgaata ccgccgtgct tacgcatcgc
     3481 tgagtagaac ggattgattg cagctcccgg aacgccgaag gcggtggtga taccttcttt
     3541 ctccagcaca tacattgccg cgtcaacggc tctcattttt gccattttat tcctacctct
     3601 attaattgga aaaattttcc aactctcaaa ttttgtcaaa aaaacccgct ccgcagagcg
     3661 ggatacgaca gacactatgg atgtgctttc agtcccaacg ccgtgctgat atcgcgggcg
     3721 gtgtctctga ccagctcacc ctggctgaca aaacgatctt ctgtcagtct tgatgaaggc
     3781 ccggagatgg agatagcggc aacaacacta ccnnnnnnnn nncagcgcct tgcccgctcc
     3841 ggaagcatgc agcggcagac gactgcccag cggcgcacac atcctgacca tcgatttaca
     3901 ctctaactga ccaattaata ccgcttcatt gccgttacgg atcgcgacat tgaccgtttc
     3961 gccggaaagt aacatcaggc ggcgcataaa cggcccggcg acggagagta catcgcggtt
     4021 atggatgtac gctgcaccga cgttaaagac acctaatcct atatgccacc agcctaactg
     4081 actgtcctga tagacaaaat ccgctgcctg taaaaccttc agcaagcgaa aggtcgtgga
     4141 gagcggcaaa tccagattga gagagatatc gctaaccgac gaacttcccc cacttttttc
     4201 cagatattgc agaatcgcaa ttccccgctc taacgcctgt gcacccttct gtgccacagg
     4261 ctccgcctgt cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac
     4321 ctacaaatga gcataaacaa tgaacaattc gtttatctta tgcaagtcgg ttgcaggcgt
     4381 cacgcaaaac agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg
     4441 cgatcgatgg tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg
     4501 ttacgatgg
//
LOCUS       DAWDRC010000321         4508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8177_length_4508_cov_2.813609, whole genome shotgun sequence.
ACCESSION   DAWDRC010000321 DAWDRC010000000
VERSION     DAWDRC010000321.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8177_length_4508_cov_2.813609"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1249 a         1115 c          974 g         1170 t
ORIGIN      
        1 atataccttg tcgaatttat atcctttcca ctctgtcatt atgcatttct gggatagttt
       61 ctactgcaac cgcaacttct tcagaaacaa aaatcagcaa cgaagagacg ctcgtcgtga
      121 ccacgaatcg ttcggcaagc aacctttggg aaagcccggc gactatacag gttattgacc
      181 aacaaacatt gcagaactcc accaatgcct ccatagccga taatttgcag gacatccccg
      241 gagtagagat aacagacaac tccttggcag gccgtaaaca aatccgcatt cgtggcgaag
      301 catcctcccg tgttttaatt ctcattgatg gtcaggaggt aacttatcag cgcgccggag
      361 ataattatgg tgtgggactg ttgatagatg agtctgcgct ggagcgtgtt gaggtagtga
      421 aaggtccata ttccgtactg tacggttcac aggcaattgg cggtattgtt aacttcataa
      481 ccaaaaaggg aggtgacaaa cttgcatctg gagttgtgaa agctgtttat aattccgcaa
      541 cagcaggctg ggaagaatca atcgcggtcc aggggagcat cggtggattt gattatcgca
      601 tcaacggtag ttattctgat cagggcaatc gtgatacgcc ggatggacgt ctgccgaata
      661 ccaactatcg taacaatagt cagggtgtat ggttgggtta taactccgga aaccatcgtt
      721 ttggcctctc gcttgatcgc tacagactcg cgacgcaaac ttactatgag gatccagacg
      781 gaagctatga ggcatttagt gtcaaaatac ctaaacttga acgagagaaa gttggggtat
      841 tctatgacac agacgtggac ggtgactatc taaaaaaaat tcatttcgac gcgtatgagc
      901 agaccatcca gcgccaattt gccaacgaag taaaaacgac acagcctgtt cccagtccga
      961 tgattcaggc tctgaccgtt cataacaaga ctgacaccca tgataagcaa tacactcagg
     1021 cggtcacatt gcagagtcac ttttcgctgc ctgctaataa tgaacttgtt accggtgcac
     1081 agtacaaaca agacagggtc agccaaaggt ccggtggcat gacctcaagc aaatctctga
     1141 ccggcttcat taataaggaa acacgaactc gctcctatta tgagtcagag caaagtacag
     1201 tctcactatt cgcacaaaat gactggcgat tcgccgatca ctggacatgg acaatgggag
     1261 ttcgccaata ctggctttct tcaaagttga cgcgtggtga cggagtatca tataccgcag
     1321 gcattataag cgatacctct cttgccagag agtctgcgag tgatcacgaa atggtaacat
     1381 ctacaagcct gcgctattca ggtttcgata acttggagtt acgcgctgcg ttcgcgcaag
     1441 gctacgtatt tcccacactc tcccagcttt ttatgcagac atctgcgggc ggcagtgtca
     1501 catacggaaa tcctgatctt aaggctgaac actccaataa ctttgaatta ggtgcacgat
     1561 ataatggtaa tcagtggctg attgacagcg cagtttacta ctcagaagct aaagattata
     1621 ttgcaagtct gatctgtgat ggcagtatag tttgcaatgg taacaccaac tcctcccgta
     1681 gtagctacta ttattatgac aatattgatc gggcaaaaac atggggactg gaaataagcg
     1741 cggaatataa tggctgggtt ttctcgccat atatcagtgg caatttaatt cgtcggcaat
     1801 atgaaacttc aacattaaaa acaactaata caggagaacc agcgataaac ggacgtatag
     1861 ggctgaaaca tactcttgtg atgggtcagg ccaacataat ctctgatgtt tttattcgtg
     1921 ctgcctctag tgcaaaagat gacagtaacg gtaccgaaac aaatgttccg ggctgggcca
     1981 ctctcaactt tgcagtaaat acagaattcg gtaacgagga tcagtaccgg attaacctag
     2041 cactcaataa cctgacagac aaacgctacc gtacagcaca tgaaactatt cctgcagcag
     2101 gttttaatgc agctataggt tttgtatgga atttctgatg acaaaaacaa tcgttatgga
     2161 attctttgtc ctgatctgcc cgctttgcgg gcaggctaac gaacgtattg tgtttgttga
     2221 aggtttttcg acgttgtttt ccatctgggg gtgaatattt attcttcccg ccagcatgac
     2281 tctatagtca ttgctacgac aatgacttat aaagctggtt actgagccta tacctttgta
     2341 attcaaaagt gatatcagtt cacaacgcaa tactattttt agattattta ctctcaccca
     2401 gatactgaat caatggataa taccttttga tttatcccga ctcggaagaa ggtgggataa
     2461 atattctcct tctattgccc cttactccaa ctatcatatt ttacaatatc aacgaaccgt
     2521 ccatgaaggg ctgaaattct cttcctgaaa cagctctgtc agcccgctaa tctgtttcag
     2581 cctcagcact tcatcctggt ccatgcccaa ctctgtgcca atacgctcat ctgtccagcc
     2641 cagacgcgaa aggtccctga caatgtcaga cattgacgtt atctggtgtt ttcccctggc
     2701 acggttatgc cgtatggttg cggcaatacg tgatgcctgt ccctttcttt ccggattaat
     2761 acaggtcact ggcagccagc ccttcagacg ttttctcgta tccgctttcc tgcccagcaa
     2821 ctgacgatga aaaccatcca cgaccagata atgctccttc tcttccgaaa ccaccacagg
     2881 ctgcgtgaat ccatcctgct ccagtgaatg tttaagcagt tttttttcac tggaagacat
     2941 gacattggga ttgtaatcgt tggccaccac ctcatccgct ttcacccaca acacacagtc
     3001 cacaggctcc tgtgcaaaag ggctgacctc atgcaacacc ctgcgcagat gattaagggc
     3061 tcgtatggtc tcttcttcag atcgttcctg actgaagtac tgtcgaatca tgttgctcaa
     3121 tacttccacc tcaggtgtca cagaatcccc cattccttcc tcctttcttt catacgctgc
     3181 agataacgtt catagtgccg gggtttgttc ggactgaagg agagggtccg gcaccagaaa
     3241 tcattcttta tgagtgtctt acagatacgt cgccaagacg ggatatcccg gctccccagg
     3301 tcattctcct gttcatccgg gatgtcatcc gggaagcccc gcgtctgata ccagcgcagg
     3361 tagacagcaa ttttattacg gtaatgttct gccgttcttt ccggcatcac atccagcagg
     3421 aacatcgcat agctacgcca ggtatgatga ggcggcttcg tgatacgctt acgcagggca
     3481 aaataggcac cgctctcatt ggcataaagc gccccgctgg cagcacccga cacccgctca
     3541 cacatcctgg cccaagtttc cggctccaga acatggtaaa gccacagtcc cttacgctgt
     3601 tccgggccaa aaggctcaca gacccgcatg ttgcgtaacg gcacgccggc acggtacatc
     3661 aggtcataca ggggattgta gatagctcgg gttctggcgt tatatatcca tatatcacgg
     3721 gctttccagt catacaacgg atacaaggtg taataaaacc cttcaggtga cgctgtggtc
     3781 cagggtttat catctgcata tctcagtttg cgctgagaca ccagtcccat aaagcgattg
     3841 agcgattcat cagcacgaac accagtcagt attgccactc cacaccggtt accggcaaac
     3901 caggaagaaa atgccggaac aaattcttca aacgtcatgg cataccgata aaatgggaaa
     3961 tacgacatat ctgtaatagc gtcatctggt ggctgacgaa cccactccac acctggttcc
     4021 cagcatatcc attccggctg aaactgagag acaccgttta ccgtagtcag ggggagtgcc
     4081 acccagtaaa aagtctccgt cacatcccga tacatctccc tcatcttcag aatgtgtgca
     4141 attgtgcact ggtactgagc ttcccagtca atgaataata cagagaaacg acgtttcttc
     4201 ctgcgagcca catcggccgt aagatggagc agcacagtag agtcttttcc tccagaaaaa
     4261 gacaaacaaa cagatgaaaa tgtctcaaac aaccactcaa tacggtgaat ggccgcatgt
     4321 aatacattaa catctgttct tatcttctgt atggttccca tacatatcct agtataaact
     4381 atcttggcag taatattctg aactacaaac ttaaaccttc tcatcaagaa aaagttctac
     4441 cttaagggta tattgttact atcacgagtt catgatagtt aagtttttag taaaacacga
     4501 catatgac
//
LOCUS       DAWDRC010000322         4499 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8198_length_4499_cov_2.174842, whole genome shotgun sequence.
ACCESSION   DAWDRC010000322 DAWDRC010000000
VERSION     DAWDRC010000322.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8198_length_4499_cov_2.174842"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3182..3191
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1130 a         1241 c         1116 g         1002 t
ORIGIN      
        1 acatcgtcca gttttttgtg gtccagttct ttcacccact tagcaacgac aatggtcgct
       61 acgccgttac cgaccaggtt agtcagcgca cgagcttctg acataaagcg gtcgataccg
      121 aggatcagcg ccagacccgc tacaggcaaa tggcccaccg cagagagcgt cgccgccagc
      181 acgataaaac cactacccgt tacccctgcc gcccctttag aagaaagcag caacacgatt
      241 aacagcgtga tttggtggac gatatccatc tgactgttag tggcctgggc gataaacacc
      301 gccgccattg tcaggtatat cgatgtgcca tcaagattaa acgagtagcc tgtcgggatg
      361 accagcccca ccaccgattt gcggcagccg agtttctcca tcttgtcgag catacgcggc
      421 agcgccgact cggaagatga agtccccagt acaatcagca gttcttcacg gatgtaacgg
      481 ataaatttga agatactgaa accagtcgct ttagcgattg aacccaatac cagcaccaca
      541 aacaggatac aggtgatgta gaaacagata atcagctgcc ccagttgcac cagtgtgccg
      601 acgccgtatt taccgatggt aaacgccatt gccccgaacg caccaatagg tgccagacgc
      661 atgatcatat tgatgatgcc gaaaatgacc tgcgagaaac tttcgatcac gttaaaaatc
      721 agttggcctt tgctgcccag acggtggagc gcaaaaccaa acagtacggc aaacagcagt
      781 acctgcagaa tgttaccgct ggcaaatgcg ccaatgacgc tcgccgggat gacatccata
      841 atgaaggcga caatgccctg gtctttcgcc tgatcggcgt aaaccgctac cgctttcgca
      901 tcaagcgttg ccggatcgac gttcattccg gcaccaggct gcacgacgtt aacgatgata
      961 agaccaataa tcagcgcgat ggtactgaca atttcaaagt aaagcagtgc gactgcgccg
     1021 gtacgaccga ccgccttcat gctttccatg cccgcaatgc ccgttacgac ggtacaaaag
     1081 atgacaggag cgatgatcat cttaatgagc ttaacgaacc cgtcgccaag cggtttcatt
     1141 tgctcgccta tttcagggta gaaatggcca aggagaatac caatggctat cgctgtcagg
     1201 acctgaaagt aaaggctttt aaacagagag gttttcatag ggtgtccttt agtaaaacca
     1261 caggtcttgt aaggttatgg ggtacctgcg gccttaaaat aacacccaga caacatcaca
     1321 gaaatgtacc tggatcataa ttgaaacaaa aaggttaaaa agtttgagct ggctcgcaca
     1381 aaccagcact ttttaaagtt ttgtaatcag tttggggtag ctacttttct tccaggtaac
     1441 tctcttcgaa gatttcaata gggagtgggc gagcaaaaag gaagccctgg gcaataccaa
     1501 cgcccgcttt tgccagccag tcgcgttgtg cttcagtctc cacgccttcg gcaatcattt
     1561 gtaggttcag gctctgcgcc agcatgataa ttgcagcaat catgctgcta tcttccggca
     1621 aaccttcaac aaacattttg tcgattttca gtacgtcgat tggcaacgat ttcatatgct
     1681 gcagctgacg cagccctgcg tagcccatgc cgaaatcatc cagcgccacc cgaactccgg
     1741 cattgcgcag cggacggagg attgccaccg cagcatgagg atcgtcaata cgtcggcttt
     1801 ctgtcacttc cagaatcagt gttcccggct gaatgcgata gcgggttaac agttccagca
     1861 tatccgccac catatttgga tgcatcagtt gcagcgctga gaggtttacc gacaagggca
     1921 gcataatgcc gcgctcttgc caggcagcaa gcaatcgaca ggactcttcc agcacccagt
     1981 gaccgacggt aaccatcagc ccacagcact caatgcgatc gattaagcca tcgggcaggt
     2041 cccaactgcc atccggttgc tggatacgca gtaacacttc cgcactgacc agtttaccgc
     2101 tggtcatctc gacctgtggc tgtaaccaaa tcgcaaactg atgattttcc agtgcattaa
     2161 ggatatcgct ctcttccgtc aaccgcttct gggcggcttc catctgctgc agatcaaaga
     2221 actgaatctg atttttgcct ttatggcgag cagtaaatgc cgcagaaata gcgcgactgt
     2281 aaagctgttc ggcggtgaga tcgccgtaga acatcgccac gccaatgcta cagtgcgggc
     2341 ggagttgaat acgttcaatc ggcaggcgct cgctaatgat agtgagcact tgctgaccta
     2401 atgtgatagc gtgccacggt tcctgtacac cgttggcaat gacagcaaag tcataaccgc
     2461 taatctgcgc gaggatcatg cgtggcgaca gtaccgattt gagtttttcc accagcgtca
     2521 gcagcagaat ttctcgttgc gcctctttca gcacacccgc agtatcacgc agggtttcac
     2581 aggtgatgat catcagcgcg gtagtttgtt tacgcgccac cacctgctcc agcatctcca
     2641 tcagcaaggc tttgttcggc aaatccgaca ccgggaagcg catcgcattt tcgttctgtt
     2701 cttcataatg gcgctgcagc aattgctggt tgaggttgta actgcgcacc aacataccga
     2761 tttcatcgtc ctgatgcaga cgcggtaatg ccagttggtg accaacaagc tcctggggtg
     2821 ggatggcgtt aagttcgcga gcaatattgc gtaacggatg caaaatcagg cggttaatgc
     2881 accagctaat cgccacagtc aatattaacg acaaaagtaa gtaaatggtc actaacgttg
     2941 agagggtgct catcacgaac ttatacatgc ggaaggaatc cgcctgtaac accagatagg
     3001 caattggctg cgggtttgcc ggacgttcca gcgagtaaac gcccagcgag atttgcaccg
     3061 gtagctcaaa caggcgagta accattaccg gcaccggacg ctctggaata aaacttttac
     3121 gcagcgcctg gaactggtta ggcagcacta catcggcacg gctgaccacg cctgccggtt
     3181 tnnnnnnnnn nctggcaaga atagcttccg cttcgggaat atcgcctttc aaaatggcag
     3241 aagataaggg ttcgcggaca gagcgagcaa tgctttccag ttgcgtagcc gtgttatagc
     3301 gattctgctg gaccagatgg aacagcaaaa cggtgcaaaa aataaaaacg aacaccagga
     3361 caacggctgc caccattgcc atctgcttga ttgttaacga gcgacttacg cgcaaattgt
     3421 ctccccccgg aaatcccaga ttgccgcccg agtatacccg atcgcagcgg cattaagaga
     3481 ggcgctatct gaaaacttac cagtcggcgt aaggtatcag cggctgcggc ggtaaatcca
     3541 tgtcaccctg ccatccggcg gcggaataac gtacgtagag caggaaatgg ctgggtgcgt
     3601 aatcttttgc ctgctggata tcaattgccg tgccgacaaa ccagttggaa gtaacacgtc
     3661 gttcaagtaa tgcccgcgcc gtgtagccaa agccctgact gctgccgcca tcgttggatt
     3721 gccgcgcagc ctcttcctgc cagtcggtcg ggatcagatt catcagcgga taacgcggca
     3781 tggttttggt gcgtgaatgc gaccacgagc cagacgcccc cagctcccac gaccagtttt
     3841 ccgtgcgctc ccgccacatc accggtatgg caaacgacag gtattcctgc ggactgtagt
     3901 aaccgccctg accgagtgag tagccactca ggtctttgtc ataatgccag atcatgttgt
     3961 tcaggccgat tgtgacgcgg cgattgttct ggttgatgac cttatagtaa tagcccgtca
     4021 tccagcgcac gcgccagtta tcttcgacat ttttaccggt taactgatca ccgctaagcg
     4081 atgcccagac gccgtttgct tcacctttat cgtagctcag acttagcccc acgccgtcgg
     4141 cacgcacgcc gccccatttt ttcccggtat tgctcggaga gtctttttgc ccaccaaagg
     4201 ccagcaaaga actggagata ggccgacggt gggcgttaac ggtgtaaccc agcggcccga
     4261 tatcatcgct gtaactgatg ccgccgacca catccaccac gttgaagccc atcggcgtgg
     4321 taccgatatc ccagctccag acgtcatttc gccagccgac cgccacgctg gcaccggaat
     4381 ccgactggct gcggttgccg ctacagtcct gtaatgtaca ggtgccccag ttgtcatccc
     4441 atttgccatc ggcattagtg gagaaactgc cgacgttcat attgacgaaa taactgcga
//
LOCUS       DAWDRC010000323         4495 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8204_length_4495_cov_2.611937, whole genome shotgun sequence.
ACCESSION   DAWDRC010000323 DAWDRC010000000
VERSION     DAWDRC010000323.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8204_length_4495_cov_2.611937"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2121..2130
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1316 a         1038 c          978 g         1153 t
ORIGIN      
        1 gtgtcttcag ctccgatgac ggtaaggcga aagtaccgtt cccggcgacg ctttccttta
       61 ttacccgcaa cggagctaca aaaacctacg atgccgggtg cgatgatagc tggcgggata
      121 tgaccgatgc gctgtggttg accacaccgt ggactgatat ctctggcgaa gtggggcaga
      181 tggataagac cacagtcaaa ttttcgattc caatggataa cgccatttct ctgcgtacgg
      241 tagatgataa cggctggttt ggcgaagtca gcgcttcagg agagattcat gttcaggcga
      301 cgtggcgtaa cattaactaa ggccctgctg acagtggtct gtatgctggc ggcacctttg
      361 acacaggcga tttcggtcgg caatctgact ttttcactgc cgtccgagac tgactttgtc
      421 agcaaacgtg tcgtgaataa caacaaaagc gcgcggatat accgtattgc catcagtgct
      481 attgatagcc cgggcagcag tgaattgcgc acccgaccgg tggatggtga actacttttc
      541 gccccccgcc agctggcgtt gcaggctggt gagagcgagt attttaaatt ttactatcat
      601 ggcccacggg ataaccgcga gcgctactac cgggtctcat ttcgcgaggt ccccactcgt
      661 aacctgacaa aacgcagccc aagcggcggt gaggtcagca cggagccggt ggtggtgatg
      721 gataccattc tggtagtacg accacgtcag gttcagttta aatggtcctt cgatcaggtg
      781 accggaacgg taagtaacac cggcaacacg tggtttaagc tactgattaa accaggatgt
      841 gattcgaccg aagaggaagg cgatgcctgg tatctacgtc cgggagacgt ggttcatcag
      901 cctgagttac gtcagccggg gaatcattat ctggtctata acgacaaatt cattaagatt
      961 agcgattcct gtccggctaa gcccccttcg gcggactaag acttatccat cggacgaggg
     1021 aagaattcca cctcagagac tcaaaaatcg tttaaatgat gtggaagcga tcgtcaatgt
     1081 caccgatgag ctttctcata tgcacaccac agtgttcagg agacacggaa aggtaccagg
     1141 cggcctgcta acgagtggtt tcttcactaa ttaggacgta aaatcggtaa cggctggaaa
     1201 tcattcaata ctcacactat cgaaagtaca ccagccaacc gcgatacgtc cctgcatacg
     1261 acgtaccgca gttctcttta cgatttatag ctgtactggt gaattatgag caatctgaat
     1321 ccatgcatga cgtgtggtgc ctgttgtgca tttttccgcg tctcttttta ctgggccgaa
     1381 gccgacgatg ctggcggtac cattcccgcc aggctcactg aacaaatatc cccttttcac
     1441 cgatgcatga gcggtactaa tcagaaaaat ccccgatgta ttgcccttgc aggaacaccg
     1501 ggcaaaaatg cctgctgcac gatatatgaa aatcgatcgt ccacatgcag agaattcgcc
     1561 atgtctggtg agaacggaga agtcaatgag gcttgcaatc gtgcaagagc taaatacggg
     1621 ctaacaccgt tataaatata caataattaa ttgcattgcc ccgccagttg taatagcggg
     1681 gcttcgtttt tcagggacaa caaaacctga aacttcttcc attttccagc aacaatgcct
     1741 ctttttctgt agcgttctcc ggatctacat catcctgaag tagcgcgata tgatcactga
     1801 ctgtccgcat cagaatatga aaagcaacgt ttttaaccta taaaaatggc gctatatttg
     1861 cgccattttt atcattcaaa gcattatcta tttgagccta aagggatctc aggatctggc
     1921 tcatgggtaa ttctgtttcg taaatctctg cgaataattt caatagacca gaaccagact
     1981 aaatgtccaa aaatttcaga gacattctca taccacggaa gatcaaagag aggtggcgta
     2041 agtcccatga gagggaatga aatcatatga acaaaaagtt gcgctaaagc acctgccagt
     2101 aagccctgcc aaagtttaat nnnnnnnnnn aaatacttcg gcgaccacac agtaaccaac
     2161 ggcaaaaact atcgaaaaga ttatgtgcgt tacaccaacc cagttaaaga catgcccggc
     2221 gaaggtataa acagccgaat ttggatctgt cagtcccaac caatcccgaa gaaaaatgta
     2281 cggaggattg agaaagttac gggagcaatc aatttggcct gcagccctga ttaatgattc
     2341 cggaccacac gctgcattaa acatatcaac cggactacgt ggtggcaatg gaacttctgc
     2401 cccccactta acaaatgcgg aaacaacgcc agcaatcagc ccaatgaatg cagcaagacc
     2461 ataacgtctg cggtttggtg gagtttgttc aaatatattc atatctaccc tgcttgtacc
     2521 attatgttat acacctcttt aggagtattc ataaaacaag gcaaatgtaa agaactgtat
     2581 tgttttgtat aaccacatgg ttacctaatc gccaattaat ataagctcca ttatttctcc
     2641 atattttcat gttagaagtg acaaggagaa atgtaatgtt tctctcagag aatataaatc
     2701 attccttttg cccaccttaa tggtgggctt ttttatgcaa aacctgaacg tcctgtggta
     2761 ttcctctggc ataatgattt tcgcgattaa ccaacaacca gaccagcaaa gacgccacca
     2821 caagcacagt aacatccatc aggcttgcca caccccatgc gtgcggctta aagccgccat
     2881 ggcatattaa tccgcttgcc attttcgaac agctgattgg tttggtccgg tgtttccctg
     2941 cttgcactta aaaatgagcg gataggaaac tctcgaaatg gttcgtcgat atgctcatct
     3001 cgcacctaat caccttaccg aacacgcacg gtaaatagat tcgatcctga acccatcggt
     3061 cccaaatctg tcccaatcaa aaaataagga aggtattaac gatgtgtaac ctattgattt
     3121 aaaatagata atataaacag attgtaaaaa gcaagacatt gatatgtata taaaaaaact
     3181 attacgcaat cattagatac ccccagattt agttgtaaag caagcacact ttgcatttcg
     3241 agacaatcca ggtaaagact cttacctccc attttgtgat ttttatactt atgatgataa
     3301 gttgaacgtt gggtagtgct tccaatcagt agaacatgtg cttttcaatg aaggaaccga
     3361 tgactttttc gtggatttca acggaacgcg agaagcagat tgttttactt accagccgct
     3421 tgatgcgggt gcggagggtc aggttgttac gttcaatacg ttgcgtgaag attttgccgg
     3481 tcaggtattt ctccttcggc acttctctgg cgtagctgcc ccagtcatcg cggccaccat
     3541 gccgatacag aacggagtca acagggctaa caattcccga caggcctcat cattccgtgg
     3601 cccgaaagtg taagccaaca caccgccagt tttagtgttc tacgcatacc agagccagtg
     3661 ttgttgcgcc ttgttgccaa cgaagctcca cagctcatca agttcacaga taagggcaac
     3721 atcagcgtga gctgactgac gcagatgtta tccgctgggg cgagagcttt ttaatgtccg
     3781 tatgacggta ttgatgctga ttttcagtgt tctggcgata tcacggcccc tgtgccatta
     3841 tgcgccattt caacgatcag ctccttaaag ccgggttttc tggcttcata agagtaagtg
     3901 agctggaaaa cacgtttaca tgaacgacaa cggaaacgct catgctggga acaactacga
     3961 ccatgtcgat aaactttatc ggaattacag cgcggacgat gaacagtaac ggtagccatg
     4021 gaaaacctta aataccagta gattaccaga aacttctatc aattgaagac attaccgttt
     4081 ttgattacag caaatgtttt attaattacc ccaccatcga ctaaatccgt tagaacgtcg
     4141 aatgatgcca ttcgtaaata cattaaaaag actgttgtaa tcaacgaaga tggaaaacag
     4201 tgtgttaaaa ctaatctgaa cttcagaaaa acagatgatg gtatggtgat atatatatcc
     4261 gctctgaacc gccacgcaat ttaaaataaa aatcaacaaa tatcgtcgat atccatatat
     4321 accatttttc tgtagttgta ttagcgtcgc atattttatg actttaagtc ataagtcaaa
     4381 acacctgaga aaaagcaaac aacattttca ttaccaccaa tgaaaaccac agacaaagaa
     4441 tgcgtgcaac tacaaaatat tgtagtttta agcaaaaata aatgtgacat atgtc
//
LOCUS       DAWDRC010000324         4489 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8217_length_4489_cov_2.012630, whole genome shotgun sequence.
ACCESSION   DAWDRC010000324 DAWDRC010000000
VERSION     DAWDRC010000324.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8217_length_4489_cov_2.012630"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2035..2044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2326..2335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1259 a          900 c         1035 g         1275 t
ORIGIN      
        1 gcgctgaagg atgattatgc attgatcatt ctggatatta tgcttccggg tatggatggc
       61 tggcagatct tacaaacgtt gagaacggca aagcaaaccc ctgttatttg ccttactgca
      121 agggattctg tcgatgacag agtcagaggg ctggacagtg gggcaaatga ttatctggta
      181 aaaccttttt cattttctga gttgctggca agggttcggg cacaattaag gcaacatcac
      241 actctgaatt caacattaga aatcagtggc ttaaaaatgg actctgtcag tcaaagtgtg
      301 agcagggaca atatcagtat tacactgacg cgcaaggagt ttcagttact ttggctactg
      361 gcctccagag ctggcgagat tatacccaga acggttattg cgagtgaaat ttggggaatc
      421 aactttgata gtgataccaa tacggtggat gtcgccattc gcaggctccg cgcaaaagtt
      481 gatgatcctt ttcctgaaaa gctaatcgcc acaatccggg ggatgggcta ttcattcgta
      541 gcggtaaaaa aataatgaaa agattatcta taaccgtccg tttaaccttg ctttttatat
      601 tgctgctgtc tgttgctggc gctggaattg tatggactct ctataatggc ctggcaagtg
      661 agttgaaatg gcgcgatgat acaacactca ttaaccggac agcgcagatc aaacagttgt
      721 taattgatgg ggtaaatcca gatacgttac ctgtgtactt taaccggatg atggatgtta
      781 gtcaggatat tttgatcatt catggtgatg gcatcaataa aattgttaac cggacaaatg
      841 tcagtgatga catgttaaat aacatacctg ctagtgagac aatcagcgca gcaggcattt
      901 acagaagcat tattaatgat acagagatag atgctttccg aattaatatt gatgaagttt
      961 cgccatcatt aacggttact gtggctaaat tggcttcagc cagacataac atgcttgaac
     1021 agtataaaat taatagcatt ataatttgca ttgtcgtcat tatattgtgc tcattattaa
     1081 gtccgttgtt aatcagaacg gggttgcgag agatcaaaaa gttgagtggc gtaacggaag
     1141 cgctgaatta taacgatagc cgagtgccgg ttgaggttaa cgcattaccg agagaactaa
     1201 aacctcttgg gcaggcgttg aataaaatgc atcatgcttt agtcaaagat tttgagcgcc
     1261 ttagtcaatt tgctgacgat ctcgctcatg aacttagaac gccaattaat gcattactgg
     1321 gtcagaatca ggttacgctc agtcaaacca gaagtatcgc tgaatatcaa aaaacaattg
     1381 ccggaaacat tgaagatctg gaaaatattt cgcggttaac agagaatata ctgtttcttg
     1441 ccagggcaga caaaaacaat gttttggtga aactggatac gctttctctc aataaggaag
     1501 ttgaaaatct tttggactat cttgaatatc tttcagacga gaaagagatt cgttttaagg
     1561 tcgagtgcaa tcaacaaatc tttgcggata aaattttact gcaacgaatg ttatcgaatc
     1621 ttattgttaa cgccattcga tattcaccag aaaaatcgcg tattcatata accagttttc
     1681 ttgatgccaa cggttctctt aatattgata tcgccagtcc tggaacgaaa attaatgagc
     1741 ctgaaaaact cttccgtaga ttttggcggg gagataattc gcgtcattcc gtaggtcagg
     1801 gacttggcct ttctttagtc aaagcgattg ccgaattaca tggaggaagt gctacatatc
     1861 actatctcag taagcataac gtgttccgaa ttacgttacc gcaaagaaat taacatcggg
     1921 cttcagtacg ccataagtta accgtgaaaa aacgcagcca cgtcgtgagt attaacgtgg
     1981 ctgcgattga ttatgcgctt acatcacaat gtaacaggga ttaactcgcg taagnnnnnn
     2041 nnnncatttc ctgcgccgcc agtttaccca acgcattcgc tgcaaaagga ctgtcgccgg
     2101 tgagaagttt acggtcctta tgtactcgcc cggtgatgtc gtcattaatg atgttcatgc
     2161 ccattttctt caactcttcg ccgaagtacc aggtgagatg acccggcata tagccaatat
     2221 ctggcgtttg tttgtctgcg gcgtctggga atgcgcaaat agaataacca ttcagtgggt
     2281 tatcgctgtg gcgaagtgcc agaaaagccg ccggaccgtg gcaaannnnn nnnnnataac
     2341 aaaacggtca tttttgattg cccactgtaa agcggcagcc acgtcctggc tttctggtaa
     2401 accaataagt gcgccatgcc caccaggaac aaagattgct gcatattcgc tgtcagcgtt
     2461 gaggctggcg acaacatccg cgagtttctt cggattacgg aataacgatt tatgctgttc
     2521 aaagaatggc attacttttt catctttgtg cggcatagcc cagtattcaa acttggtcat
     2581 cagaccggaa atggtcgcca cttcgaattc gaaacctgca gcatggagat gatacaacgg
     2641 cagcaacgtt tcaatcggat ggttaccggt cgagaacagt tttccgttat cggtcggcaa
     2701 ataacgttcg tccgcggcga tcaccagaat tttatgttta ccgcgatacg gttttggata
     2761 gtccacgcca tcaagatcag agacgggact cgtatattgg ctaagcgaat attctgaagg
     2821 gaagaacgca ttatcttcag caatatcgac ctgcgggttt ttacttgttt gaacagtcat
     2881 agtgtattcc ttattgttgc ttagttaggg tagtcactat atttgcgact gagctggtcg
     2941 ctatttttaa ccaggctgaa aaattgagtc aatatatcga aagcggtagt tggcgggcat
     3001 aagtatgtaa tgatgatgct gcactggtgt tgaatattat tcacgccaaa ctttccttat
     3061 tgagtaacgt cgtcgcaagt ggcctcaatg attttagacc attatcagag gtttgggggc
     3121 gatggggaga gatttagtgc tgtggaatgt tgtggtactg atgaatattt atatcccgga
     3181 ttggtaggga aaaagctttt ttcatgcact atttttgcaa atatttctat atgggaagcg
     3241 gttttgttac agattaaatt tataaagaaa tgcatttggg gtgttggctg gaagagttgc
     3301 tggtagagga tattgtgcca ggaggggcag ggtaagtggc tgtatcgaga tgctttggca
     3361 attgcgtgtg ccgcagatgg agatgaaatg ccaataaaaa aagcctgagt tcgatgaggg
     3421 acgaactcag gctggtgtta tgttttacag taacacgcct cggcgcgttg gctgattaga
     3481 actggtagac gataccaact gccagttggt cgtcggtgtt cagtccatga gcgagaacat
     3541 aatcactctt atcaagcagg ttgatctgat aagcggtata tacgttcata ttcttattga
     3601 agtaatacca ggtaccgatt tcaatatagt tcagacgatc agaatctttg taacctgcta
     3661 cgtccagtgc tttagaatag gagtaaccga tggatggacg cagaccaaag tcgaactgat
     3721 attgcagaac agcttcgaag ttttgtgttt tgttcacaac atcaccgtcg tcggaattca
     3781 tgttgtgaga ttcaccgtac atcacagcag catagacatt gttagcgtca tattttgttg
     3841 acaacgccca aacttcggct ctttcgcctt taccatcacc agactgccaa tcagtacggt
     3901 cagagtttgc ataagcacca gcaaatgaga aaccgtcaat agtgtaagtt gcagataaac
     3961 catggccatc gccatttgct ttgtagttgc ctgtaccttc atttttaccc tgatattgca
     4021 gagcaaaatt tagaccttca accatgccaa agaaatctgt gttacgatag gttgcaacac
     4081 cgttggtacg gttggtcatg aacaggtcag taccagccca tgagtcaccg ccccactcaa
     4141 caaacatatc agtaaatgct tctgcatcat acgcgacacc gacattacga ccgtagtcga
     4201 atgaaccaaa atctttgtaa ctcaaaccag catatgccaa acgagtttgg tcagggttgt
     4261 gtctgtttga tgcttccagg tctagttcaa actgaccgta accagtcagt tcagggttga
     4321 tctgagtttc acctttcacg cctacacggg cataagaggt atcttgagaa ttatcgcctt
     4381 tttcaccatt ttcacggtcg gtcagaatgc gctcaccaac catcttgccg tatagctcaa
     4441 ctttattgcc atttttatta taaatttcag ccgcatttgc tgcgccagc
//
LOCUS       DAWDRC010000325         4472 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8253_length_4472_cov_2.396876, whole genome shotgun sequence.
ACCESSION   DAWDRC010000325 DAWDRC010000000
VERSION     DAWDRC010000325.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8253_length_4472_cov_2.396876"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1103 a         1106 c         1240 g         1023 t
ORIGIN      
        1 tcattgaccg caccaacgcg gcagcttaag aaggaattga acatgtcatt accgatgttg
       61 caagtcgcgc tggacaacca gactatggat agcgcctacg aaaccactcg cctgattgcc
      121 gaagaagtcg acattatcga agtgggcacc attctttgcg taggcgaagg cgtgcgtgcg
      181 gttcgtgacc tgaaagcgct gtacccgcac aaaatcgtac tggctgacgc caaaattgcc
      241 gatgcaggca aaatcctttc tcgcatgtgc ttcgaagcca acgctgactg ggtgaccgta
      301 atttgctgtg cggatatcaa caccgccaaa ggcgcgctgg acgtggcaaa agagtttaat
      361 ggcgacgtgc agatcgaatt gaccggctac tggacctggg aacaggcgca gcagtggcgc
      421 gacgcaggca ttgggcaggt ggtttatcac cgcagccgtg acgcgcaggc cgcaggcgtg
      481 gcatggggcg aagcggacat taccgcgatc aaacgtcttt ccgatatggg cttcaaagtc
      541 accgtcaccg gtggtctggc gctggaagat ctgccgttgt ttaaaggcat tccgattcat
      601 gtgtttatcg ctggtcgcag cattcgtgat gccgcttctc cggtggaggc cgcacgtcag
      661 ttcaaacgtt ccatcgctga actgtggggc taaggagcgg aacgactgca actggcaaaa
      721 acgttaggct tcgattttgt cgagatgtca gtagatgaaa cggacgatcg cctttcgcgc
      781 ctcgactgga gccgcgagca gcgtctggca ctggtgaatg cgattgttga aaccggcgtg
      841 cgcgtaccgt ccatgtgcct ttctgctcat cgtcgtttcc cgctgggcag cgaagatgac
      901 gcggtgcggg cccaggggct ggagattatg cgtaaagcta tccagttcgc ccaagacgtc
      961 ggtattcgcg tgatccaact ggcgggctat gacgtttact atcaggaagc caataacgaa
     1021 acgcgtcgtc gtttccgtga cggtctgaaa gagagcgttg agatggcgag ccgcgcgcag
     1081 gtcacactgg cgatggagat catggattac ccattgatga actccatcag caaggcgctg
     1141 ggctacgcac actatctcaa caatccgtgg ttccagctct acccggatat cggcaacctg
     1201 tcggcgtggg acaacgacgt gcagatggag ttgcaggccg gaattgggca tatcgtcgcg
     1261 gtacatgtga aagacaccaa accaggcgtc ttcaaaaacg tgccgtttgg cgaaggcgta
     1321 gtggatttcg aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg
     1381 attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtagcgaa agcgcgtgat
     1441 tgggtgaaag cgcgcatggc caaagcaggc atggtggagg cggcataatg caaaagctga
     1501 aacagcaggt gtttgaagcc aacatggatt taccgcatta cgggttggtg acctttacct
     1561 ggggcaacgt cagcgctatt gaccgtgaac gcgggctggt ggtgatcaag cccagcggtg
     1621 tcgcctacga aaccatgaaa gcggacgata tggtggtggt cgatatgagc ggtaaggtgg
     1681 tggaagggga gtatcgtccg tcttccgaca ctgcgacgca tctcgaactc taccgtcgtt
     1741 atccgtcgct tggtggtatt gtccataccc actccactca tgccaccgca tgggcgcagg
     1801 cggggctggc gatcccggcg ttaggcacca cgcacgccga ttacttcttt ggcgacatcc
     1861 cgtgcacgcg cggattaagc gaagaagagg tgcagggtga gtacgaactg aataccggca
     1921 aagtgattat cgaaacgctg ggtgacgccg agccgctgca tacgccggga attgtggtgt
     1981 atcagcacgg accgttcgcc tgggggaaag atgcccacga cgcggtgcat aacgcggtgg
     2041 tgatggaaga agtggcgaaa atggcgtgga ttgcgcgcag tattaatcca caactgaatc
     2101 acatcgacag cttcctgatg aataaacact tcatgcgtaa gcacggtccc aacgcctatt
     2161 acgggcagaa gtaaaacacg cgctgcggaa atttccttct tcgggagata actggtctaa
     2221 ttccgcagcc gtttttcaaa aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat
     2281 gtttcgttgg ttatacctgg tgctagcgat aaatatccgc gctggcgtgc atattgccgt
     2341 tactccccgg ttcccgcatc agaatcacat ggtagtacgt tgcgccttgc gcatctgtct
     2401 cttcatttaa tgcctgacgt gcttcacttt cagtggcgaa gttatggttg atataaatca
     2461 cgcctaagct ttgcacatcg tccatatttc tggcctggtg gttattaatt tcaatggctg
     2521 cccatgtatt tgcacttagc aaaagcacag ccagaagggc taaaacgcga ctgaacatag
     2581 atacctcctc gacggctgac tttgtgtgct ctcctcttgt ggtgatcttc tgatttaatt
     2641 ttaatcaatg ataaagaagt tgatggtgac catttctgat gcagttgttc aaaaaaacac
     2701 catgacgaag tgtgatgaac tccaaatcag cggtttagag gttatttgcg aaaggggaga
     2761 tttatttcgg ctctgccctt gagtttagcg gggtatgtca gtactataac gcgtcatttt
     2821 tcagccgacc tttaacacgt tccttgcctc cccgggattc ggctgaccca gacaggaggc
     2881 tgaataatcc gtaaggagca attcgatgcg tcattacgaa atcgttttta tggtccatcc
     2941 tgaccagagc gaacaggttc cgggcatgat cgagcgctac actgctgcca tcactggtgc
     3001 agaaggcaag atccaccgtc tggaagactg gggccgccgt cagctggctt acccgatcaa
     3061 caaactgcac aaagcacact acgttctgat gaacgttgaa gctccgcagg aagtgatcga
     3121 tgagctggaa actaccttcc gcttcaacga tgccgttatc cgcagcatgg ttatgcgtac
     3181 caagcacgct gttaccgaag catctccgat ggttaaagcg aaagacgagc gccgtgagcg
     3241 tcgcgatgat ttcgcaaacg aaaccgctga tgatgctgaa gctggggatt ctgaagagta
     3301 atttctgatg accaaccgtc tggtgttgtc cggcaccgtg tgcaggactc ccgttcgaaa
     3361 ggtcagtcca tcaggaattc ctcactgcca gttcgtgctt gagcatcgtt ctgtgcagga
     3421 ggaagccggc tttcaccggc aggcatggtg ccaaatgccc gttattgtta gcggacacga
     3481 aaaccaggcc attactcaca gtataacggt cggcagtcgc ataaccgttc aggggttcat
     3541 ttcatgccat aaggcaaaga acggactgag caaaatggtt ttgcatgccg agcagattga
     3601 attgatagat tctggagact agccatatgg cacgttattt ccgtcgtcgc aagttctgcc
     3661 gtttcaccgc ggaaggcgtt caagagatcg actataaaga tatcgctacg ctgaaaaact
     3721 acatcaccga aagcggtaag attgtcccaa gccgtatcac cggtacccgt gcaaaatacc
     3781 agcgtcagct ggctcgcgct atcaaacgcg ctcgctacct gtccctgctg ccgtacactg
     3841 atcgccatca gtaatcggtc acggtccatt aatacgactt tgagaggata aggtaatgca
     3901 agttattctg cttgataaag tagcaaacct gggtagcctg ggtgatcagg taaacgttaa
     3961 agcgggctat gctcgtaact tcctggtacc gcagggtaaa gctgttccag ctaccaagaa
     4021 aaacattgaa ttcttcgaag cacgtcgcgc tgaactggaa gctaaactgg ctgaagttct
     4081 ggcagcagct aatgctcgcg ctgagaaaat caatgcactg gaaactgtta ccatcgcgtc
     4141 taaagctggc gacgaaggta aactgttcgg ttccatcggt actcgcgaca tcgctgacgc
     4201 tgtaactgca gctggcgttg aagtggctaa gagcgaagtt cgtctgccga acggcgttct
     4261 gcgtaccact ggcgaacacg aagtgagctt ccaggttcac agcgaagtat tcgcgaaagt
     4321 gatcgtaaac gtagtagctg aataattcgt tattcaacga gacgtaaaaa cgccgaccag
     4381 tggtcggcgt tttgctttta agtgataaaa accgcagaaa cagacgttcc ctccataaat
     4441 ctcttgttat tccttttata aaaagttcgc ag
//
LOCUS       DAWDRC010000326         4462 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8273_length_4462_cov_2.275244, whole genome shotgun sequence.
ACCESSION   DAWDRC010000326 DAWDRC010000000
VERSION     DAWDRC010000326.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8273_length_4462_cov_2.275244"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    551..560
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1673..1682
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2226..2235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1072 a         1241 c         1061 g         1058 t
ORIGIN      
        1 aagccagatc acggcgtact gttccggcgg cggcgcaagg taatgtaacc gacaacgact
       61 acgtaatgtt gccagtaaac gttctggctc gcgggtagca aggaaaaacc acgtttctgc
      121 tggtggctct tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtta gtaaggcagc
      181 atcggttacc cagacaactt tcgcaccacc taagcgtgcg tgctcattca gcttttcggt
      241 gacctcacgt accgcatcaa tgcccagcgc atttttccct ttttcgggag ccagggtgta
      301 gtaatcggga tgtgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt
      361 gtggccctgc ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc
      421 gcccatgccc ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct
      481 ggctaccagt ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc
      541 ttcacccagt nnnnnnnnnn aaatcgaatg actcttgctc aatacgatcc agctcaccac
      601 gggcgcgggc acgttttaag ccgacctctg gggtgacatc aagataaagc gttaagtcag
      661 ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg tcaataccac
      721 gtcctccacc ctgataggct tgagtggaga gatcgtggcg atcgccaatc acccaggtgc
      781 cgttagccag cgcaggtttg atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca
      841 tcaaaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata tccagcacca
      901 ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc
      961 ggatacccag ttgctcaagc gtctcaacca ccacgttacg cgcggtagtt ttgcctgcgc
     1021 cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag cactttcaga
     1081 taatcctgca cagacttgtt atgactggca agattggtat taaacgtgtg accaccttta
     1141 ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc cttcagcgaa
     1201 tccgcccccg gcgtcgctat cgcacctagc ggcagaccgg taatggtata ggtgttatac
     1261 gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc tcccatcccg
     1321 taaatcacgg tcgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt gataaatact
     1381 gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat tgatgccatc
     1441 gtcaccaact ggtttttatc tttataaggc agaccgtccg cacgcccttc ccaggcgcta
     1501 tcgaccgctt tcaccatttt cttgtgcgct cgcttgagta acgcgacatc ggtggtattg
     1561 gcggtataca tccaggtgtc tggccagaac caaccttcaa tccactccgg gttttccagt
     1621 tcaagtgcct gcgctacggt ggcgtactta tcgtcgctca gcgtgtgctt gannnnnnnn
     1681 nngtgcttga tatacggagc ctcacgcaat tgcttgaggt agtcgctcag acgcatccct
     1741 tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt ccaacaattt cagcatctcg
     1801 cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt taaagtgaga aagatccggt
     1861 tcgatacgca gcaaccattg aaaaacccgt gggcgattga tgatcttatc ggcataaagc
     1921 tgttcaccta gcgccagacg tccggtccct ggcttcaggg taaatatcgt ctcttctttg
     1981 ataagcaatt tgctgtcggc aagatggcga accttccaga cgcccacacc agcggcgata
     2041 cccagtacca ccagcaataa caagattatc aataacactt ttttcatgac taattcgggc
     2101 gctcacaaag tggggctaaa tattcatata acgttgctga cgaaaaggag acatcgccac
     2161 aggcacgtac gggcatcact ggcattaacg cattacaaat aaccatctca tctgcctgca
     2221 acgccnnnnn nnnnngattg tgccagcaaa cggatacaga attgtcgcat aatgccgtta
     2281 acacctgcct gatccagtcg cggcgtataa actacgttac ccttccgcca gaacaaatta
     2341 gccgcacagc attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt
     2401 gtctgctcaa gatgagagcg gatcaatact tgctcaagcc gattaagatg tttaatacct
     2461 gcaagatgag gattgcgccc cagccgcacc gggcttagcg ccaacatcat cccttcgtta
     2521 cgcaaacggt cgtaatgtgc aggataagcc gtaacggaga gaatccgcgt tgccggtcca
     2581 ctgttcaatg tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagtaca
     2641 ccattttgct gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca
     2701 caggaaatca tcaaccgctg acaagcatcc tgtagtcgct ggatatgcgc cgataacaaa
     2761 ctgactttac cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc
     2821 cgatcgctta ctgtcagcga ttcctgctta tgaccgttaa ttaagaacat accggctcct
     2881 ctccttatca gagaattgtt agtgtggcag catgttcact acggaacaag tcggaataaa
     2941 agctaagaaa aaaggcccgc aagcggacct tttataaggg tggaaaatga caacttagat
     3001 ctttttaaag atcaaagaac cattagtgcc accgaagccg aaggagttac acagagtgta
     3061 ttccattccg ctaacctgac gcgcttcgtg cggtacgaaa tccagatcgc aaccttcgtc
     3121 cgggttatcc aggttgatgg ttggcggaac agcctgatcg cgcagcgcca ggatggagta
     3181 gatagattct actgcacccg ccgcacctaa caggtgaccg gtgctagaat tcgtggagct
     3241 gacccacacg cggcttgcag cttcaccgaa gatggttttc accgcctgcg cttcagcttt
     3301 atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat
     3361 gcctgcatca cgcagagcat ttgccatcgc cagagctgcg cctgcgccat tttctggcgg
     3421 tgacgtcata tgataagcat cgctgctcat accaaagccg acgagttcag cgtaaatttt
     3481 cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc agcataccgg caccatcgcc
     3541 cagtacgaaa ccatcacgct ctttatccca cgggcggctc gccgcttgcg ggttatcatt
     3601 gcgggtagat aatgcacgtg ccgcgccaaa accaccaacg cccagcggcg tactggcttt
     3661 ctctgcgcca cctgcaacca tcacgtcagc atcgccatac gcgataatac gcgcagcatg
     3721 gccaatgttg tgcacgccgg aagtacaggc ggtcgcgata gagatgcttg ggccacgcag
     3781 gccatacatg atagtcagat gacctgccac catgttcaca atcgttgacg gaacgaagaa
     3841 tgggctgatc ttacgtggac caccgttcat cagagatgtg tggttttctt cgatcagtcc
     3901 gaggccgcca atcccggagc caattgcggc accaatgcgg gttgcgttct cttccgttat
     3961 ttcaaggcca gaatcctgca aggcctgaac gccagcgaca attccatatt gaatgaaggc
     4021 atccatcttg cgctgttctt tgcgcgagat aatgtcctca cagttaaaat cctttactaa
     4081 gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg tcgattaggc tgatgccact
     4141 ctgaccggca agcagagctt tccaggtaga ctctacggta ttgccgacag gagacaacat
     4201 gcccagtccg gccacaacta cacgacgctt agacacgttt gtcctccagg gagggaaaaa
     4261 tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc
     4321 acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca
     4381 gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg
     4441 gtgtcaagag aatccgcgcc ca
//
LOCUS       DAWDRC010000327         4458 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8280_length_4458_cov_2.070407, whole genome shotgun sequence.
ACCESSION   DAWDRC010000327 DAWDRC010000000
VERSION     DAWDRC010000327.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8280_length_4458_cov_2.070407"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1135 a         1138 c         1041 g         1144 t
ORIGIN      
        1 aagaaacaag atctgaccag cgaagagatc accaaccaca ttgaagccgg aaaagtggtg
       61 actaaactgg cgctcgactg gcagcaacgc attcagtttg tgatatgcga cgatggttcg
      121 ctcaagcgtc tgaagttctg cgacgagctg cgcgatcaaa acgaagatat cgaccgtgaa
      181 gatttcgccc agcgctttga tgccgatttc atcctgatga ctggtgaact ggcagcgtta
      241 attcaaaacc tgattgaagg attaggtggc gaagcacaac gttaattgct gatttacctt
      301 taatgccgga tgcgacgcct gccgcgtctt atccggcgta caaagccaca ccaggaatat
      361 aatcgttagc tacagcgggc aataattctt agcgcagcaa tattatgcgt tttatgctgt
      421 aacttgctcc aggggcgttg tgtcattgtt tttcctcaag ccggtgacgc gcttcactgc
      481 gaattttccg cgttgccccg tttttcacta aatcacgtaa cagcgaaaaa tcgggttgtt
      541 cgacaaataa gagtaatgat ggctcctctt ttaaccacgt cgttttcaca tccgcagcca
      601 gctgtgaatt attaatagcc agcgaccgct cctgaggctc agtcaatgtc gttaacaaca
      661 ccggagtatg tgcgttgcgc aatgccgttt ggcgcaaatg atcattgcga ctttgcagca
      721 atgcacgcca ccattcatca tctgatttat cttttatcgt cgccagttcc gcaacaatat
      781 ctggagatct tcgctgatat ttatatcaac cttgatgaat tatgaatata tcgaaaatcg
      841 aaaaaatccc agcaaaggaa atattattat gggttaaaga gaggacattt cagaaaaatg
      901 ccctcccgtt caaggggaag gcgaggaatt acagatagcg gcacagataa gaggtgggtt
      961 cggcaacttg cagatgaaac tcgctgtgac cgggaacatt aaacaccgaa ccggcttcat
     1021 acacttgcca gtcggtcgcg tcaggcagta acacattcag cgcgccactg attaccgtca
     1081 tctcttcagg ctcagcggtg ctgaaggtgt attcaccttc aaccataaca cccacgctgg
     1141 cgcgaccagt gctgctgctg gaaaagccga tcgatttcac tttgccggaa aagtactcat
     1201 tactttgaag cataaactgg ccctgtctgg tagttgaaca tctacttcac tataggggcc
     1261 agaggcgctg gctgtcacgc aaaattacat cagtaattcc gcagccagcc gtgcaatcaa
     1321 tacgttagag agcaagacag gaacatcgag ctgtttttgc agtaaatcgc gatgacgttg
     1381 gtgaaatcct aaacaatcca gcatgatgac atcagcccct tttgccagta attctttccc
     1441 ggcatcaatg attttttgtt ctgaatcatg aatggggtta cccaatgaaa atactggcgg
     1501 tttctgcaaa atttgccatt tttgcgcctg aacgggcagc atctcctcaa ccggtacgat
     1561 aacccccacc tgatgatctt caacaataga ggaaaccagc ggtggcaata tccgcgacgg
     1621 ctcaagaaag atcgtattac gcgcggtcat actactaatg tttgctgtac tcattaatat
     1681 aatgacgtcg taaccctgat tatcgagcac ttcaaccaca tcttgcaggt ctcgctccac
     1741 tttgcgacgc gaaacatggg ccagatggtt gtcatttaat aatgtgagaa ttgtgtcttc
     1801 gcctgcttct ggcgcgtact ccgccatcac ttcttcgcgg cttaacttcc ccagcaggct
     1861 atgatgggaa atattatctt cgtcaatgta ttctgtcagg agcggcaaaa cttcctgcat
     1921 aggtacaatg ccgatagtca ggatcgccaa cgacgcactc attgttcttc caccttactt
     1981 ttatttcgtc gttaattacc gggcaagtgt taagcaccac tgctgacact tacttgtgct
     2041 acaaatgaca aaaagcgtag cagcagacct gcactgcata atgtaaagat tccaaatgat
     2101 tccagtaatg gattgttatt gtttaatatt ctaattattt gaaaaacatg aattgtgaaa
     2161 aatgtgacgc agatcccttg tggcggcact cagaatccct tccgagcagt ctgatgcggt
     2221 cactctttgt cagggtcctc atagcgtttt ttcgcatcca tcgcttcctg agcggtcgga
     2281 tgttcactga tcaacgagtc tggtttagga tgatcggctc tgagttggta ccaggttacc
     2341 gtctgtcctg gctttccttt ttcaatcgtc actatatatg cttcacgagg ataaggcggt
     2401 ttcgttggca tcgttcttcc ttatttcacg ggatgaacgt taagtatagg cgctcgaaaa
     2461 tcaacaattg atcgtctgtg ccagagcgct gcgaatttca gaaatcacct ggctgggttc
     2521 gtttgttgcg tcgatgataa tatgcgcaac ttcgcgatat agcgcatcgc gttcttccag
     2581 cacttcctga acttcttcgc tcagtggttt tcccgttaag gttggccgta aatcttcttc
     2641 cggtgcagct tgcagtcggt taaccaggac tgatactggc gcacacaaat aaaccacgat
     2701 cccgttattt tgcatgaagt gacgattaaa ttccgtcaga ataatgccgc cgcctgtagc
     2761 gataacggtg gatggcgcag ttaccgcttc cagcgccgcc gtttctctgg cgcggaatcc
     2821 cgcccactct tccctttcga cgatctccgc gaccgtcata ttgagctgtg attgcaacca
     2881 caggtcggta tcgacaaacc gacggttaag cgaatcggca agggccattc cgaccgttgt
     2941 tttaccacag ccccgaggcc cgatcagaaa aagaggttgt gtcatcgtgg gttttcccca
     3001 ataggtcgca atgcggcgaa agccggtgtc atgagaatag cgatcatacc atcaaactag
     3061 tacaatttcg attgtaaaga aaaaattcca cttaaggtgg aaatctcaat acatccctta
     3121 ctataccaat aaatattcaa gaatgaagtg taaataataa attacattta gccacgacta
     3181 cgttgcactt ccagccacca cttctccagc tccgcagcaa aagcctggcg gtcacgttgt
     3241 gaaagactat ctggtcctcc ggtctggatc ccactgacac gtaaggtatc cataaaatcg
     3301 cgcatcgtca ggcgctcacg aatggtcgct ggcgtgtaac gttcgccgcg cggattaagc
     3361 gccgccgcgc ctttctcgat ggcttcagca gccaaaggta tatctgcggt gatcaccaaa
     3421 tcgcccgctt cacactgccg aacaatttcg ttatcagcaa cgtcgaaacc tgccgcgacg
     3481 cgcagcgtac gaataaatcg cgatggcggc acgcgtaaac tctggtttgc taccagtacc
     3541 agcggcatct gcatacgttc cgccgcgcga tacaaaatct ctttaattac attgggacac
     3601 gcgtcggcat ccacccaaat tgtcataaag tcatcctttg ttgggtaatc ctctattgtg
     3661 tcgcgctttt gccttccggc atagttctgt ttatgcttct gccagcgatt atcaaaacaa
     3721 tgaatttcac ggcaggagtg aggcaatgga aaagaaaatc ggttttattg gctgcggcaa
     3781 tatgggaaaa gccattctcg gcggtctgat tgccagcggt caggtgcttc cagggcaaat
     3841 ctgggtatat accccctccc cggataaagt caccgccctg catgaccagt tcggcatcaa
     3901 cgccgcagaa tcggcgcaag aagtagcgca aatcgccgac atcatttttg ccgccgttaa
     3961 accaggcatc atgattaaag tgcttagcga aatcacctcc agcctgaata aagactctct
     4021 ggtcgtttct attgctgcag gtatcacgct cgaccagctt gcccgcgcgc tgggccatga
     4081 ccggaaaatt atccgcgcca tgccgaacac tcccgcgctg gttaatgccg ggatgacctc
     4141 cgtaacgcca aacgcgctgg taaccccaga agataccgct gatgtgctga atattttccg
     4201 ctgctttggc gaagcggaag tgattgctga gccgatgatc cacccggtgg tcggtgtgag
     4261 cggttcttcg ccagcctacg tctttatgtt tatcgaagcg atggccgacg ccgccgtgct
     4321 gggcgggatg ccacgcgccc aggcgtataa atttgccgct caggcggtaa tgggttccgc
     4381 aaaaatggtg ctggaaacgg gagaacatcc aggggcactg aaagatatgg tctcctaaca
     4441 gagagtcacc accattta
//
LOCUS       DAWDRC010000328         4455 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8288_length_4455_cov_2.317045, whole genome shotgun sequence.
ACCESSION   DAWDRC010000328 DAWDRC010000000
VERSION     DAWDRC010000328.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8288_length_4455_cov_2.317045"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1179 a         1096 c         1110 g         1070 t
ORIGIN      
        1 cgatttgctg gtgctgctgc gcgtttatct gggctggcgc tgtcaggccc gtctgcaact
       61 gacgctgccc gtcagcctgt tgcctgctgc ccggctggga aaacagcgtg tacagattag
      121 ccgtaccggc atccttcgcg cctcctttgc cgcgcccgcg accggaacag tgacggtcag
      181 tctggggcgt tatcagggat taatacccgc tttttccatt cgtaacaggg agagtatgac
      241 tcatgtttcc tattcgtttt aaacgtccgg cgttgctctg tatggcgatg ctgacggttg
      301 ttctgagcgg ctgcgggctg attcagaaag tggtggatga atcgaaaagc gtggcctcag
      361 ccgttttcta caaacaaatc aaaatactgc atctcgattt cttctcccgc agcgccctga
      421 atacggatgc ggaagatacg ccgctttcca cgatggtgca tgtctggcaa ctgaaaaccc
      481 gcgaagattt tgacaaggcg gattacgaca ccctgtttat gcaggaagag aagacgctgg
      541 agaaggacgt actggcaaaa cacaccgtct gggtaaaacc ggaaggcacg gcatccctga
      601 atgtgccgct ggataaagag acgcagtttg tcgccattat tgggcagttt tatcaccctg
      661 atgaaaaaag cgacagctgg cgtctggtga tcaaaaggga cgaactggag gccgacaagc
      721 cgcgctcgat tgaactgatg agaagcgacc tgcgactgct gcctctcaag gataaataac
      781 cgatgtcgac accttcctta tatgaaatgc tgacgttcag tttcagcggc gagttaccgc
      841 tggaacagat cagcgagcgg gatcagctta tcctttccgt gatggacaat atgcaacgca
      901 ttatcaactg ccgggcaggc acgctggcgc atttgccgga ttacggtctg ccggatctca
      961 gtctgatcca tcaggggatg gctgcgggta ttcatggcct gatgcgtcag attgaagaaa
     1021 cactgctgcg ttatgaacca cgcctgagtc agatacaggt ggaattactc ccccagcccc
     1081 gtccggggca tcttaattac ctgatccacg cgcagcttcc cgataccggc tggatacgct
     1141 ttgatggcgt attttctccg gaaggacgaa ttgttctgcg tcatctcaaa caacaggagc
     1201 gggcgtactg atggcaagta acgcgaattt tatcagccag ttcgtcatgg gcggcgatcc
     1261 ctgtacttat aaggaatccg gtgaactgca ggctgaaatg agtaaactga ctcacccggc
     1321 ccgaccggat gtggactggc gtcaggtgga aaaactctgc ctcgcgctgt tccggcaaaa
     1381 tggcgtggaa ctacagacgc tggtctgtta cgtactggcg ataaccagac ggcaggggct
     1441 ggcagggatg gcagacggac tcggttcact ggatatactg ctccagcgct gggctgactt
     1501 ctggccggtg caggtacatt ccagaatatc actgctcggc tgggtcacag aaaaaatgca
     1561 gcaggcactg agaacgctgg atattcagta tcaggatctg ccgcagatct accgttgtgt
     1621 acagcatctt tccgccatcg aaaccacgct gcaacagtgt gaactgtggc atatgacgaa
     1681 actggatcta ctggccgggc agtttcgcaa taccgcattg cgtctggaac ggctggcgcc
     1741 tcagggagcg gaaaccacta tcactccccc tgaattaccc cgccgggaaa tgaatcaacc
     1801 gaaaaagtca gaggaaagtc cacagccggt ttttgcaacc agatccgttc agcaaaacga
     1861 taaggatgcc agtccacccg ttccatcccc tgaaatctcc cggcagcgga catggccgat
     1921 atttatggcc ggaatggttg tgatggccgg tctcggcgga acaggattat ggggctggtc
     1981 gcagcttaat cagccggacg cgctaatcca gcgaatacaa ctgtctgtca tgccgttgcc
     2041 gctgtcgctg gagagcggcg aactggcaaa gctggatgta aaggataagg cgctgctggc
     2101 tcaggacaga acaattgcgg caagtcagat gcaactggag cagttaaaca aattgcctgc
     2161 ccgctggcca ctggagcagg gatatcgcca gctacgccag cttgatgccc tgtggccgga
     2221 taatcctcag gtcagggcgc tgaacgcgca gtggcgcaaa cagcgggagc tgagcgccct
     2281 gtctgctgag gcactgaatg gctatgctca ggcgcagagc cagctacagc gcctgtcggc
     2341 gcagctggat gcactggatg aacgtaaggg gagatatctg accggttcgg aactaaaaac
     2401 ggcggtgtac ggcatccggc agtcgttaaa ggagccgccg ctggaagaac tgcttcggca
     2461 actggaagag caaaaacaga ccggagaggt ttcgccaacg ctgttgacgc aaattgatac
     2521 ccggttaaat cagttgttga atcgctatgt cattttactg gatacgaagg tggaacaaag
     2581 tcagtaaaca tcgggggatt tatgtaccaa taagggatat gtgaccttgc cattttttaa
     2641 aggagtctct gtgtacaaaa aaagagagga acaggccatt cgatctactc gttgattcac
     2701 atcagataat gagtgtatat ttcggtagat agcttgggtt cgggaacagt gaatacctta
     2761 agtttcaaaa gattggatta acctcttgac tgtggccccc gcaaccaatt tattagaatg
     2821 ctgacaaacc gcaatgtgtt ctacaaattg cggttttttt gtatttaaaa tctttgttcg
     2881 ctgaactcac aacacgtttt ttatctccct tcctgcgaag taatcaatta tcgactgggc
     2941 aacgtgtatc ccggtttgct ggtatgactc aacggtggcg gccgcgatat gcggcgttgt
     3001 aatgatattt ttggcatgta gcagtgggct aaattgcgcc aacggttcag agctaaacac
     3061 atccagcgcc gcaaacacat ttcgttcttc aatcacctga agtaaagcgc cctcatccag
     3121 caactttccc cttgcgacat tgatgatcag agcaccattg cgcatcatcg acaaaattta
     3181 tgactaatga agttctccag cccagaagaa aaaggcaaaa gaacaatgat gacatgcgac
     3241 tgcataaaga tatcttcaat tgatacaaac tgaactccag ctttgtcagc aacctgatag
     3301 tcgggttgtt tgtcaaaagc taaaagctca acatcaaaac cggataacct tttcgccaga
     3361 gttttaccta tattaccgta gccaaccagg ccaatccgtt tcccttgtaa ttcacagcca
     3421 tgcgactcac cccagtagcc attttgcatc gcatgatagc tgccagggat atttctcatg
     3481 ctcgcgaaga taagtccaat gataaattcc gcaaccgcat ttgaattaat cccaaccgca
     3541 ttattgacaa caataccgga ttgctgtgcc gcacgaagat caatattgtc tatccccgtc
     3601 ccgaatctcg atattattct cacaccaggc agcaatgata gcgtgtccgc ctgataacca
     3661 tcttttccgg cgatgaccgc gcaaatagat tctcgtaatg cagtggattc tgatttaagc
     3721 agatgctcca cattattctt aataataaga ttaaaattat tctctcttag caacttgctg
     3781 gcggaaacac agcgtgctga aaagctgggg acggtgacca gaacattttt catagcggac
     3841 actcacttca aagaacgcca ccccaaaaga tggcgtgacc ggttactgta tttatatttt
     3901 gaaattatga atttgcttta atgccgagtg cacctcctgg atgatataag ccgaaatcag
     3961 cacgagtaaa ttttttcaat tcagagagtg caagcgccac cggtgctaaa cctaaattat
     4021 ccgcctcact tttgacgggg atttcaacag ataaatcaca acttatcgca agcgatgagt
     4081 gatgactgcg ggtaaaagag atcaaataat tacccatttt tttcaaacta ggcagtgtcg
     4141 ccaggatttc cgccgtctca ccactgtttg aaataagaat aacaacatcg tctttcgcca
     4201 ccatcccaag gtcgccgtgt accgcttccg taccgtgaac aaaaaacgag ggagttccgg
     4261 tactggcaaa agttgctgcg agttttctgg caataatacc ggacttacca acaccaataa
     4321 aaacaacttt tccctgacaa gattgtaata ctttaattaa attagcgaat tcagaaagtg
     4381 gggaatcaat aacctcttct actgctttaa gttcgttatg cataaaatgt ttaatgagat
     4441 ggataagatc cgtgt
//
LOCUS       DAWDRC010000329         4436 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8318_length_4436_cov_2.624971, whole genome shotgun sequence.
ACCESSION   DAWDRC010000329 DAWDRC010000000
VERSION     DAWDRC010000329.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8318_length_4436_cov_2.624971"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3141..3150
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1064 a         1212 c         1123 g         1027 t
ORIGIN      
        1 ccagaggcgg attcgcccag aaaagttaac ccttcgaccc acttcactcg cgcttgcatt
       61 tttgctactc cactgcgtca attttcctga cagagtacgc gtactaacca aatcgcgcaa
      121 cggaaggcga cctgggtcat gctgaagcga gacaccagga gacacaaagc gaaagctatg
      181 ctaaaacagt caggatgcta cagtaatata ttgatgtact gcatgtatgc aaaggacgcc
      241 acattaccgt gcagtacagt tgatagcccc ttcccaggta gcgggaagca tatttcggca
      301 atccagagac agcggcgtta tctggctctg gagaaagctt ataacagagg ataaccgcgc
      361 atggtgcttg gcaaaccgca aacagacccg actctcgaat ggttcttgtc tcattgccac
      421 attcataagt acccatccaa gagcacgctt attcaccagg gtgaaaaagc ggaaacgctg
      481 tactacatcg ttaaaggctc tgtggcagtg ctgatcaaag acgaagaggg taaagaaatg
      541 atcctctcct atctgaatca gggtgatttt attggcgaac tgggcctgtt tgaagagggc
      601 caggaacgta gcgcatgggt acgtgcgaaa accgcctgtg aagtggctga aatttcgtac
      661 aaaaaatttc gccaattgat tcaggtaaac ccggacattc tgatgcgtct gtctgcacag
      721 atggcgcgtc gtctgcaagt cacttcagag aaagtgggca acctggcgtt cctcgacgtg
      781 acgggccgca ttgcacagac tctgctgaat ctggcaaaac aaccggatgc tatgactcac
      841 ccggacggta tgcaaatcaa aattacccgt caggaaatcg gtcagattgt cggctgttct
      901 cgtgaaaccg tgggacgcat tctgaagatg ctggaagatc agaacctgat ctccgcacac
      961 ggtaaaacca tcgtcgttta cggcactcgt taatcccgtc agagtggcgc gttacctggt
     1021 agcgcgccat tttgtttccc ccgatgtggc gcagactgat ttatcacccc gatatcaact
     1081 atgcacttcg acaaacgctg gtgctatgtt tgcccgtggc cgttgggtta atgcttggcg
     1141 aattacgatt cggcctgctc ttctccctcg ttcctgcctg ttgcaatatt gcgggccttg
     1201 atacgcctca taaacgtttt ttcaaacgct taatcattgg tgcgtcgctg tttgccacct
     1261 gtagcttgct gacacaggtg ctactggcaa aagatgttcc cctgcccttt ttgctgaccg
     1321 gattaacgct gatacttggc gtcactgctg agctggggcc attgcacgca aaattgcttc
     1381 ccgcatcgct gctcgctgcc atttttaccc tcagtctggc gggatacatg ccggtctggg
     1441 aaccgttgct catctatgcg ctgggcactt tgtggtacgg attgtttaac tggttttggt
     1501 tctggatctg gcgcgaacaa ccgctacgcg agtcactaag tctgctgtac cgtgaactgg
     1561 caaattactg tgaagccaaa tacagcctgc ttacccagca caccgaccct gaaaaagcgc
     1621 tgccgccgct gctggtgcgc cagcaaaaag cggtcgattt aattacccag tgctatcagc
     1681 aaatgcatat gctttccgcg caaaataata ccgattacaa gcggatgctg cgtattttcc
     1741 aggaggcgct ggatttgcag gaacatattt cggtcagttt gcatcagccg gaagaggtgc
     1801 aaaagctggt cgagcgtagc catgcggaag aagttatccg ctggaatgcg caaaccgtcg
     1861 ccgctcgcct gcgcgtgctg gctgatgaca ttctttacca tcgcctgcca acgcgtttta
     1921 cgatggaaaa gcaaattggc gcactggaaa aaatcgcccg ccaacacccg gataatccgg
     1981 ttgggcaatt ctgctactgg catttcagcc gcattgctcg tgtgctgcgc acccaaaaac
     2041 cgctctatgc ccgtgactta ctggccgata aacagcggcg aatgccatta cttccggcgc
     2101 tgaaaagtta tctgtcacta aagtctccag cgctacgcaa tgccggacga ctcagtgtga
     2161 tgttaagcgt tgccagcctg atgggcaccg cgcttcatct gccgaagtcg tactggatcc
     2221 tgatgacagt attgctggtg acgcaaaatg gctatggcgc aacccgtctg aggattgtaa
     2281 atcgctccgt aggaaccgtg gtcgggttaa tcattgcagg cgtgacgcta cactttaaaa
     2341 ttcccgaagg ttacaccctg accttgatgc tgattaccac cctcgccagc tacctgatat
     2401 tgcgcaaaaa ctacggctgg gcgacggtcg gttttactat taccgcagtg tataccctgc
     2461 aactgttgtg gctgaacggt gagcagtaca tccttccgcg tcttatcgat accattattg
     2521 gttgtttaat tgctttcggc ggtactgtct ggctgtggcc gcagtggcag agcgggttat
     2581 tgcgtaagaa cgcccatgac gctttagaag cctatcagga cgcgattcgc ttgattctga
     2641 gcgaggaccc gcaacctacg ccactggcct ggcagcgaat gcgggtgaat caggcgcata
     2701 acactctgta taactcattg aatcaggcga tgcaggaacc ggcgtttaac agccattatc
     2761 tggcagatat gaaactgtgg gtaacgcaca gccagtttat tgttgagcat attaatgcca
     2821 tgaccacgct ggcgcgggaa caccgggcat tgccaccgga actggcacaa gagtatttac
     2881 agtcttgtga aatcgccatt cagcgttgtc agcagcgact ggagtatgac gaaccgggta
     2941 gttctggcga tgccaatatc atggatgcgc cggagatgca gccgcacgaa ggcgcggcag
     3001 gtacgctgga gcagcattta caacgggtta ttggtcatct gaacaccatg cacaccattt
     3061 cgtcgatggc atggcggcag cgaccgcatc acgggatttg gctgagtcgc aagttgcggg
     3121 attcgaaggc gtaatacagg nnnnnnnnnn ggctaaacca tcggatgagg tgttcacgcc
     3181 gcatccgaca tcttttgcct gacgcgtctt atttacgccc caatcacctt cgccaccgcg
     3241 tgggcgaaac gatgcatccc ttcatcgata tccgcatctt ccaccaccag cgacggcgca
     3301 aaacgcatca catcaggccc ggcattcagc accattacgc cttcctctgc accggcatac
     3361 aggaaatcac gcgcccgacc tttgtactgt ggtttcagct ctgcgccaat caacagcccc
     3421 ataccgcgaa tatcgctaaa cacatcgtac tgctgatcga tcttctgcaa atggtcaaca
     3481 aaacactggc gtttcgcctg aataccttcc agcacttcag gggtattgat gatatcaaac
     3541 gccgcgcccg ctactgcaca ggccagagga ttaccgccgt aggtggaacc gtgagaacca
     3601 ggatgaaacg cggaagcaat ctccgccgtg gtcagcatgg cgctaaccgg gaagccgccg
     3661 cctagcgctt tcgcagaggt cagaatatcc ggcgtcacgc cgtagtgcat ataagcaaac
     3721 agatcgccgg tccgccccat cccgcactgc acttcaccaa acaccaataa tgcctgatgt
     3781 tgatcgcaca gctcgcgcaa gccctgcaaa aactctggcg tcgctgccgt cacgccgccc
     3841 tcgccctgaa tcggctcaac caccaccgca caggtatggt catccatcac ggcttttact
     3901 gcgtgaagat cgttaaaagg cacgtggatg atgtctgccg gttttggccc aaagccgtcg
     3961 gaatattttg gctgcccgcc caccgagacg gtaaacagcg aacgaccatg aaaagcgtta
     4021 tggaaggcaa taattttggt tttgaacggg ctatgacgca cacaggcgta atggcgtgcc
     4081 agtttaaagg cggtttcgtt agcttccgtg ccggagttca taaacaccac gcgttcggca
     4141 aacgttgcct caatcagttt acggccaaga cgcagcgccg gctcattggt gaaaacgtta
     4201 ctaatatgcc acagagtttc gccctgggtt tttaacgcgt tcaccagcgc aggatggcaa
     4261 tggcccaacg ccgtaactgc aatgccaccc gcgaaatcga catactcctt gccctgttga
     4321 tcccagattc ggctgccctg accttttacc ggaataaact ctgccggagc ataaatcggc
     4381 agaatcactt catcgaaagt cgcgcgtgta attgctgttt gttcaattgc catctc
//
LOCUS       DAWDRC010000330         4426 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8351_length_4426_cov_2.030199, whole genome shotgun sequence.
ACCESSION   DAWDRC010000330 DAWDRC010000000
VERSION     DAWDRC010000330.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8351_length_4426_cov_2.030199"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    810..819
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1191 a         1119 c         1072 g         1034 t
ORIGIN      
        1 tatctgaata ctgttgccgc taaccggcag atccctgctc agcaggtatt ccctggcgcg
       61 caagggttgc ttgagggttt aaccaaaacc ggtggcgata ccgcgaaata tgcgctggaa
      121 aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg aaaaaactct gaccaaagag
      181 ttcggctgga gcaagactga taaaaattat cgcgccatca gttattacga ttacgcattg
      241 aaaacgccgg cagataccgg tgacagcatc ggagtcgtct tcgctaatgg cgcaattatg
      301 gatggcgagg aaactcaggg gaatgttggc ggtgatacca ctgcggcaca aatccgcgac
      361 gctcgccttg acccgaaagt gaaagcgatt gttctgcgtg ttaatagtcc cggcggcagc
      421 gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag cccgggcagc gggtaagcct
      481 gtggttgtat cgatgggtgg catggcggca tctggtggtt actggatttc cacaccagct
      541 aattacattg tggctaaccc cagcaccctg accggttcta tcggtatctt cggcgtgatc
      601 accaccgtag aaaatagtct ggattcgatt ggtgttcata ctgatggtgt ctcaacttca
      661 ccgctggcgg atgtttctat caccagggca ctgccgccgg aagcgcagca gatgatgcag
      721 ttaagcattg agaatggcta taaacgcttt atcacgctgg ttgctgatgc gcgtcattca
      781 acgccggagc agattgataa aatcgcccan nnnnnnnnnc aaaagctaac gggctggtcg
      841 atagtctcgg ggatttcgat gatgcggtcg ccaaagccgc agagctggca aaagtgaaac
      901 agtggcatct ggaatactac gttgatgaac cgaccttctt cgacaaagtg atggacaaca
      961 tgtctggttc tatccgggca atgttgccag atgcgttcca ggccatgtta cctgcaccgc
     1021 tggcctcggt agcctctacc gttaaaagtg aaagcgacaa gctggccgcg tttaacgacc
     1081 cacaaaaccg ttatgcgttt tgcctgacct gcgccaacgt gcgttaagtc ttgtactaag
     1141 tggccgacag atcgtcggcc acattacttt ttacgtcgac gaatcctctt cccgctgtct
     1201 cgccacatat ttccttatcc tcatagtatc aggtgcgctc cccctgcctc acgtatatac
     1261 ttttgctctt tcgatatcat tcatatcaat atcatgcaaa agaaatcaat ttacgtcgcc
     1321 tacacgggcg ggaccatcgg gatgcagcgt tccgagcagg gttatattcc ggtgtcaggt
     1381 catctacagc gccaactggc gctgatgccg gaattccatc gcccggagat gccagatttc
     1441 accattcatg aatatacgcc attgatggat tcttctgata tgacgccaga agactggcag
     1501 catattgctg aagatattaa agcgcactat gacgactatg atggttttgt cattctgcac
     1561 ggtacagaca cgatggcgta tacggcctct gcactgtcgt tcatgctcga gaatctcggt
     1621 aaaccggtca ttgtgacagg gtcacaaatc ccgctggctg agttacgctc tgacggacaa
     1681 attaatctgc tgaatgcgtt gtacgttgcg gcgaattatc ctatcaacga agtaactctc
     1741 ttttttaata atcgactgta tcgtggcaac cgaaccacca aagcacatgc cgatggtttt
     1801 gatgcgtttg cctctccaaa ccttcctccg ttactggaag caggtatcca tattcgccgt
     1861 ttgaatacgc cacccgcccc gcacggtgaa ggggcattga tcgttcatcc aatcacgccg
     1921 caaccaattg gcgtagtaac gatttatccg gggatttctg ctgacgtcgt gcgcaatttt
     1981 ctgcgccaac ctgtgaaagc attgattctg cgctcatatg gcgtgggtaa tgcgccacaa
     2041 aacaaagcct tcctgcagga attacaagaa gccagcgatc gcggtattgt ggtggtcaac
     2101 ctgacacaat gtatgtccgg taaagtgaac atgggtggtt atgccaccgg taacgccctc
     2161 gcccatgccg gcgttattgg cggtgcagat atgactgtag aagccacgct aaccaaactg
     2221 cattacctgc ttagccagga acttgatact gaaaccattc gcaaggccat gagccaaaac
     2281 ctgcgtggcg aactgacgcc ggatgattaa ggagactgca atgccctcgc gccctgttac
     2341 tggtcgattt acaaaatgat ttctgtgctg gtggcgcgct cgccgtgccg gaaggtgaca
     2401 gtacggtgga tgtcgctaac cgcctgattg actggtgcca gtcgcgcggt gaagcggtta
     2461 tcgccagtca ggactggcat ccggcgaatc acggcagttt tgccagtcag cacggtgtag
     2521 agccttatac gccgggccaa ctcgacggtt tgccacaaac cttctggccc gatcactgtg
     2581 tgcagaacag tgaaggcgca caattacatc cgttactgaa gcaaaaagcg atcgcagcgg
     2641 tgttccataa aggcgaaaat cctttagttg acagttacag tgcctttttt gataacggcc
     2701 gtcggcagaa aactgcgctc gatgactggt tgcgcgctca tgtaatcaat gaattgatcg
     2761 ttatgggtct ggctactgac tattgcgtta agtttaccgt gctggacgcg ttacagttag
     2821 gttataaggt gaacgtgatt accgatggtt gtcgtggcgt gaatatccag ccacaggaca
     2881 gtgcgcacgc gtttatggag atgtcagcag ctggggcaac gctatatacg ctggcagact
     2941 gggaagaaac gcaggagtaa ttttacgctg gcctacaatt ctgtactggc attgtaggcc
     3001 agataaaaca catcagtgat acatctggta attgacgcca tcaacgaaag atttaattca
     3061 cttcagaaat ctcttccagc gacaccttcc gcgtttcgat gccaaaaacg gagagaacca
     3121 gcgcacagag cactaacatg acaccaagca ccataaacac cgtgatcgac ccataatgtg
     3181 ttaataatgc cgcaacgcca tagggcgtga agactgcgac gatccgtccg acggcattaa
     3241 cgaaacccga accacgcagg cgtaaatgcg ttggccaaag ttccgggata taaaccgccg
     3301 acgcgaagca cacatacatg tataaaaaga agatcatcac caggccatag attaaaatcg
     3361 cccactctgt agtctgaatt gaatagatat agcctaacac agcaataata ataagtaagg
     3421 cagagccaaa taatcgacga ggaaaatgat caataattaa tgccgcaata aatatcccta
     3481 ccggagcgcc aatcataata acagcggtca ttaatattga tttatcaaca tcaatgccgg
     3541 agttaacaaa tatggtcggt atccatacgg tgatggtata aagcgaaacg ttcattgcaa
     3601 ttaaaacagt aatcgcgact aatgtacgtc gtaacatttc ccctttaaac aggagccaga
     3661 aggtaccctt gattacctta accttgctgt tgctctggtt caaagtaagc gggggtaaac
     3721 gaatcctctt ctctctttca atttgctgct ctacttcacg aagttggctt tctgcacctg
     3781 cgatttgccc tttccctgcc agccatcgtg gcgactcaat aaagtattta cctgagagaa
     3841 accacgctaa cagtatgcca ataccgccca gcagaaacat tatccgccaa ctaaaaaaag
     3901 cgataaccac cacgcctatc gccgcagaga gcatgggcga ccagttacca acaaatgaga
     3961 gccgcgcgga ccattttcca cgcaccgtcg cggggataaa ctccgtaaat gaggcgtagc
     4021 caaccataat cagcgccccc attcctgttc ccatcaggaa gcgaaagaag atgagccagt
     4081 acatatcagg gacaaaagcg gcccctgttg ccgcaatacc gacgatgaga agatttatgc
     4141 gaaacgccct gcgccgccca aagtagtcac caataaaccc accggtaaga gagccgatga
     4201 aataaccaaa cattaatgcc gaggtaaagg cggcattgag gaaattattt gaccatccat
     4261 tgcttaccag ctttgctaag acgacattac cggagtaact caaaaacccc gttaataaca
     4321 gactaaagct tattatacca aatatacgat aatgaaaccg ggccaaaggc aagcggtcca
     4381 gtcttgcgcc aatttgatca tattgttcca ttgggtgcct cggatt
//
LOCUS       DAWDRC010000331         4407 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8385_length_4407_cov_1.647289, whole genome shotgun sequence.
ACCESSION   DAWDRC010000331 DAWDRC010000000
VERSION     DAWDRC010000331.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8385_length_4407_cov_1.647289"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    268..277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    560..569
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2693..2702
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3365..3374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1017 a         1180 c         1133 g         1037 t
ORIGIN      
        1 gctgttcagc ggtggtgaag aaaccaaacc gtccgagcaa ccaacaccga aagcagaagc
       61 gaaaccggaa cgtcaacagg atcgtcgcaa gcctcgtcag aacaaccgcc gtgaccgtaa
      121 ttagcgccgc gaccctcgta atgaacgtac tgaaggcagc gataatcgcg aagaaaaccg
      181 tcgtaatcgt cgccaggcac agcagcagac tgccgagacg cgtgagagcc gtcagcaggc
      241 agaggtaacg gaaaaagcgc gtaccacnnn nnnnnnntac caccgacgag cagcaaacgc
      301 cgcgtcgtga acgtagccgc cgccgtaacg atgataaacg tcaggcgcaa caagaagtga
      361 aggcgctgaa tgttgaagag caatctgttc aggaaaccga acaggaagaa cgtgtacgtc
      421 cggttcagcc gcgtcgtaaa cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg
      481 tagccgaaga agcggtagtc gcaccggtgg ttgaagaaac tgtcgcagcc gaaccaattg
      541 ttcaggaagc ggcagccccn nnnnnnnnnt tccgctgcca gtcgtagcgc aaactgcacc
      601 agaacagcaa gaagagaaca atgctgataa ccgtgacaac ggtggcatgc cgcgtcgttc
      661 tcgccgctcg cctcgtcacc tgcgcgtaag tggtcagcgt cgtcgtcgct atcgtgacga
      721 gcgttatcca acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc cggaactggc
      781 ctctggcaaa gtctggatcc gctatccaat tgtacgtccg caagatgtac aggttgaaga
      841 gcagcgcgaa caggaagaag tacaagtgca gccgatggtg actgaggtcc ctgtcgccgc
      901 cgctgccgaa cctgttgtta gcgcgccagt tgttgaagaa gtggctgaag tcgtagaagc
      961 ccccgttcag gttgccgaac cgcaaccgga agtggttgaa acgacgcatc ctgaagtgat
     1021 tgctgccgcg gtaactgaac agccgcaggt gattaccgaa tctgatgttg ccgtagccca
     1081 ggaagttgca gaacacgcag aaccggtggt tgaaccgcag gaagagacgg cagatattga
     1141 agaagttgcc gaaactgctg aggttgtggt tgctgaacct gaagttgttg ctcaacctgc
     1201 cgcgccagtc gtcgctgaag tcacagcaga agttgaaacg gtagctgcgg ttgaacctga
     1261 ggtcaccgtt gagcataacc acgctaccgc gccgatgacg cgcgctccgg caccggaata
     1321 tgttccggaa gcaccgcgtc acagtgactg gcagcgccct actttcgcct tcgaaggtaa
     1381 aggtgccgca ggtggtcata cggcaacgca tcatgcatct gccgctcctg cgcgtccgca
     1441 acctgttgag taataattag ctcaagtaat caagccctgg taactgccag ggctttttta
     1501 tttcatcttt gagtcaacat attaaaattc atcaaatggc atccttgcta accaacaata
     1561 caaaataggc aaaaaaatgc ccgctccggc gatcccagag cgggcagaca aaacataccc
     1621 agtttcatga tatgttttaa agcgagctta tttgttgagc tggaagagcg acaatccctg
     1681 catatcggta aatgctttat acgatgcctg caatgccgtt tgctgcatga tgtaagatga
     1741 aatagttgca ttccagtcca catcaaccag atcgctcatc tgctgcgttt gccctaaagc
     1801 gcgatcgcta cctaatgaat ccagcgactc cagttcgttc agctgcgtgc ctaattccgc
     1861 gcgaacagtc agcacattgt tcagcgagtt tttcagtccg cggttggttt tatccagtgc
     1921 cgcagcggcg gtttctttat ccgcttcgct atccgcgacc ggcgttttca gggctgcgat
     1981 ggcgctatcc agcatggcaa aaagattggt ttcagaagcg ctaccgtctg gttccgctac
     2041 cgcgttgcta gtaatactgt cgaaaatttt gtcgcccgta tgtcctatca ccatcgaacg
     2101 cgaagcatcg acctgttgtt taatactttc tgttccgccg acataatcac cgtcagcctc
     2161 actaaacggc gcagtctctg ttttataacc ggcaaaaatg tagcgcccgt taccgtcagt
     2221 ggtgttcgcc agattcagta actgatcacg aagcccctga atatccgtag ccagtgaggc
     2281 ccggtcatcg tcactcaagg tgccattgct ggcgtagaca attttttcct gagcattctg
     2341 gatagcagta gtgacctggc taagcacact ctcttccagt gacacttttt gagtggcgaa
     2401 agtacgcgcc agcgtgtact ggctgttttg cgcctgtgcc tgggagagaa caacagcttg
     2461 tgatgcagca atgggatcat ctgaagggtt aacgactcgc ttacccgtcg acatctgttc
     2521 gccgtacttc atccattctg cctgagaatt ggtgatgcca cgcatgtttt gctggtacat
     2581 catctgggta ctgaaacgca ttttatctcc ccttagcgaa tgttaatcag cgcatcaaaa
     2641 atcgcgtttg ccgtctgcag aacctgcgca ttcgccaggg aatacttctg aannnnnnnn
     2701 nnttattccc gatatcactc actaatgagg cataagcgtc gttaaaggat ttcgcaccgc
     2761 ccaccgtttt actgttgctt tgcagatcca gcagggcctg accgttgcgg ttgtcgctat
     2821 cacccgcatc ttcttcgctc gccatggcga ttttcgcttc gtcagtgatt aatacatcca
     2881 tgttgacgat ggcgtcactt accggtttca gcgtgaagct gtcgttaacg gcaggcgttc
     2941 ctgtaaacgt caactccaga ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg
     3001 taaaagtggt attgctggca aggcgggtga cctgccactg attattatcg aacgagattt
     3061 tgtaatctgt cgccagtacc gcggaggcat cagttaccgt ggcaccgatc gcaacgtcac
     3121 cgttgttttt cgtgttttgc agaaccgcgg gcttaccgat agcaaagaaa tctttaccgg
     3181 catcgccgtt ggcatcaaat ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca
     3241 gcgccagttg tccgagcgta ttacgcgtct ggtccagatc ctgagaacgg aatgtcagaa
     3301 tgccgcccag cgacccggta ttcagtaatt tctccgggat ctcaatattg ccagccgtcc
     3361 catcnnnnnn nnnncagtcg tacgcgaagg gtcggcgctg gaaggaactg ccgccagctg
     3421 ccgcgccgta cttccctgaa ccagtgagta gccattggcc atcgtgatgt tataagtgcc
     3481 accatcctga acgctaactt ctacgccaac aatctggttt aattcactca ccagttgatc
     3541 gcgttgatcc agcagattgt taggtgacgc ccctgccccc acgcctgtca gacgcgagat
     3601 ttgatcgttc aggctggcga tttgtttagc gtaattgttg atctgatcaa cactggcacc
     3661 taccgcgata ttgacctgtt tgtcctggtc gcgcagatat tgatcggtgg ttttaaactg
     3721 attcaccaac ccttctgatt tcccaatcag cgcctggcgc gctgccgggt cttccgcgtt
     3781 acttaccagc gtttgcaggc ttgtgaagaa atcctgcatc tgtgttgcca gcgaagaggt
     3841 actggtggag agcatattgt cgattttcga catctgctca tagcgggcag tcaggccgct
     3901 actttgcgtc tgcgccgcac gtaactggtt ggtaataaac gcatcatact cacgctgcac
     3961 accagaaacg tagacgccat tgccaaccca gccgccagcg cccaacgtgc tattggcctg
     4021 cgccataata gtggtttggc gggtatatcc ggcaacgtta tagctggaga tattattact
     4081 tgccgtattt aacgccgcct gggccgcgtt cagtccgctc atggcgttat tgatcaagct
     4141 ggacatggag gttccttata agccttcaat tacagagtat tatcggcagc gacccgccgg
     4201 acttgagtta ttcagaacag attatcaatg ttcatactgt aggttttgct cactttgtcg
     4261 cttatcgatt tcatctgctg aatcatgttg gtgagtttgc gggcatagtg aggatcggtg
     4321 gcatagcccg cgtcctgtag ggcctgtgcc ccctgctccg cactcacggc ggtcgtcacg
     4381 gcggcgtagc gcgggttacg cgttaac
//
LOCUS       DAWDRC010000332         4404 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8392_length_4404_cov_2.531616, whole genome shotgun sequence.
ACCESSION   DAWDRC010000332 DAWDRC010000000
VERSION     DAWDRC010000332.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8392_length_4404_cov_2.531616"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1146 a         1078 c         1076 g         1104 t
ORIGIN      
        1 aagccctttc cctgcgcgga gatgccgata ccgacaattt gttccccgct gacgccagaa
       61 tgggtaagca gggcgcgaat gacagcagtg caacattgcc acagttctgc catgtcgcgc
      121 tctgcccagc ctggctgtgg acttaatgcg cacagcggca ggcgctgcac gcccgcctcc
      181 cggccttctc ggtcatatag cccggctttc agccagctac cgccacaatc taaccccagc
      241 cagtattgcg tcatggttta ttgcacctca tccacggctt tcagcaggtc agcaccctgg
      301 ggaacatctt taacaaacat gtcacggacc tgagtaccca gcgcatcgct aaaggctttg
      361 cggtcgagat cggtgatgac ttccacgccc gcttctttca taccgtcgat gattttttgc
      421 tgatcttcgg cgaccagttt gcgctgatag ctacccgctt cctgcgcaga tgagatcagt
      481 gcctgttgga actccggggt taagccatca aacttcgctt tgttgatcac caccagaagc
      541 ggcgaatagg cgtgatgggt cagagaaagg tacttctgca cttcataaaa ttttgctgac
      601 cagacgacgt tgatcgggtg ttcctgagcg tcgatagtgc gggtttccag cccggtatag
      661 acttcggcaa acggcatcgg gatcgggtta gcgccaaaaa ctttaaatgc ggcgatattc
      721 atcgggctgt tgttggtgcg gattttcaac cctttcaggt cggcaggcgt tttgaccggt
      781 gcacgcgagt tggtgacatc gcgccagccg ttttcccagt aggccagtac tttcagcccc
      841 ttaccttcaa gggagacttt caggtcatca ccgactttgc cgtcgagcgt tttatgcgcg
      901 tgagcggtat cgcggaacag gaacgggaca tcgagcaggt tgatgacggg cgagagtccc
      961 gtaaagttgt tagagccaga catctccata tcaatagtgc cgccacgcac gccgctgatc
     1021 atcgcctgcg cattaccaag ggtgctatcc gggaacagtt ttaatttcag ttcgcctttg
     1081 gttttttcct gcagtaattc attaaatttt ttcgccgcaa tatgttgtga atcggtttgt
     1141 gaggtttcat aaccgaaacg taatgattgt gccgccagag aagatgtgct gaacgtcgcc
     1201 actccggcaa ttaataaagc gtaggttaca gaacgtaatt tcattatgat ttccttttaa
     1261 ctcatccatt taagaggagc ggtaatgagc tccggtataa aaataaataa tgctaacaac
     1321 atgaataaaa ccatgacgta aggcgcaacg ccttttaccg catcgtcaaa tttaagtttc
     1381 gccacgccgc ataccacgtt aagaacatta ccgactggag gcgtaatcag tgatattgaa
     1441 cagttgataa taaacatgat gccgaaatag accggatcta ttcccgcttc tttcactaat
     1501 ggcattaata ctggtgtaag aattaatacg gtaggcgtta agtccatgac cgtactgatc
     1561 accataatag cgagcataat gacgatgaat aataagcgag gcgaatcaac caacggttgt
     1621 aatagctccg ataccatcat gggtaattcg gcgatcgtaa taagccatgc agataccgca
     1681 gcagaggcca ccaggaacat caccacggat gtggtttttg ccgcgttaat cagcacatga
     1741 taaagcgtgg agaaggtcat ctcgcgatag accaccaccg agacgaacag agcgtagaag
     1801 gcggcaaccg cgcctgcttc ggtcggtgta aataaccccg aacggaaacc gccaataata
     1861 attatcggta aaaagagcgc ccaaataccg gaaactaagg attgccagac ttctcgcagt
     1921 gaggctttcg gctgacaagg taaattcagt cgttttgcct gccaccacca ggtaaccatc
     1981 agcgtgacgc ccatcatgat gcccggtgcg atgcccgcca taaacagttt gctgatggag
     2041 agtccgctgg agacgccgaa gataatgagt ggtatggaag gcgggataat ggccgcaata
     2101 atccccccgg aaccgataag cccggcggcg cggtttaccg gatagttggc ctggcgcatc
     2161 atcggcacca gcagcgccgc gacagctgcg gtatcagcaa ccgcagaacc ggacaggctt
     2221 gccatgatca tcgctgccag cacgcccacg taacccagac cgcctggcct gtggcctacc
     2281 agtttcattg gcaagtcgac gatacgttgc gacaggccgc ccgcgttcat gatctcaccc
     2341 gccagtacaa agaaggggat ggctaacagg gagaagctgt ctgcgccatt taccaacgtt
     2401 tgtgccagaa tttgcacatc aaagatatcc atccagaaca tcaatgaaat gccgcacagt
     2461 aataatgccc aggcgatggg aacgccaatt gcgatagccc ctaacaaact gacaataaat
     2521 ataaggattg tcatcgtcag gctccttgtt ggtttcggct gggggtattt gtgaactgcc
     2581 ggtataagtg acgcaattcc agcaatgcga taatcagact ggtggggata gcggcggcgt
     2641 acattaagcc aacggggacg ccaagaatgg gcgataagtt gctccagtct tgcgctgttt
     2701 tttctatagc gccccaggcc agccccagac acagcaataa aatgagcgta tgggttagca
     2761 gcgaaacacg ttgttgatta gccggtgaga gtttttcgac taaaaaagta acctgcacat
     2821 ggctgttgtc catataagca acgattgcgc caataaaggt taaccagaca aataaaagtc
     2881 gggataattc atcaatcgat aatatacttg tgtggaatcc atatcgaaga atgatattaa
     2941 taaaaataat acaagacagt attgcgatga tgatcgcaag aatgccttcg agtatccgtt
     3001 tcataacgag tccttctggt tatgcaatat tttttatgcg ctaatgttgt taatattcgt
     3061 aataattatc agaaaacggc taattagata ttcaaagtga tatataaatg gttctgttta
     3121 acgagattca ttcagagagt gaatgccgtt gcgtaatatt tcgatgcgat ggtgcacatc
     3181 agccgtcgca tttatttcca gtagcttgat cccagcaaaa tgcaatgttg acgattctgc
     3241 tacattaaat aactcgcgaa catgatctgt ggtcatcagg ctttctgcgc agaatggtgc
     3301 aaacggtgcg tgtaaatcct gccattcgcc gtatgccccg tttagcgtcg taccagagaa
     3361 cattaacgcg ccgagttttc cggcttgttt cgccatttgg gtgtgggtca gcggcagcgt
     3421 tgtattgcgc ccttcaatgg cagagcgcgc ccagttgata cagatgctga tgtcataatc
     3481 ggtaataact ttcagcacat tttctaaaag caaaaagcct tttcgggggg aagaagatgt
     3541 catggcgtcg cagtgttcca gcaccagctt gcacggccag tcccaactgg cgatttcttt
     3601 caatgagcgg gcaaaagcgt ccgtcgcttg cataacatta ggattcgttg ctaacggtgc
     3661 agcctggagc tccagtgcga gcactttatt agcctgcaat gagttgattt tattgaacag
     3721 gtggcgatag tacgccacgc aggcctggcg ctgttcttca tcactggagg cgagaccaaa
     3781 cccgctgttt tcgctgcggc gacgcatggt ttccatcact gcggtgacga caaatttcca
     3841 gctctctggc gtatgacgga gtaaccattg atcgccaaga gggtgaaaat tttccaggca
     3901 aggttgttcc agccccaaaa tgtctggagt ctcagataat tgtcgccaga aatccttctc
     3961 ttcctcttca cttctttggt gaaaagaagg tgcacaggga tacgcgccga taatataacc
     4021 ggcattattc gttttcatta tgtgctccag taatcatttt ttataaagca gcgagggcca
     4081 ctttaaccac gatcttgcgt attgccgtag tggcattttt attacatgcc gggcgatgaa
     4141 catcctgcgg gaagaaaata gcgtagctgc ccggaatcat ttctataaat gattcatgtt
     4201 cactatcgtg ataaaaaata atatcgcgct gctctaatag tgattcgctg actttattat
     4261 ttcccgtatc aatagcaatg ccgattttct cttcacccca cgccagaaac tgaatatcga
     4321 tataccgacg atgcacttcc ggacggtttt ccaccgcttc gcgtgtggtt aaatcgataa
     4381 tttgcgcata aatattcttg ccgt
//
LOCUS       DAWDRC010000333         4381 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8442_length_4381_cov_2.092695, whole genome shotgun sequence.
ACCESSION   DAWDRC010000333 DAWDRC010000000
VERSION     DAWDRC010000333.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8442_length_4381_cov_2.092695"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1930..1939
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2465..2474
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          993 a         1139 c         1209 g         1020 t
ORIGIN      
        1 cgtttcattt cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttac
       61 ccattggcgc aagctactct cgtttcagtg gctttcatta tgtttctttt gtgaatcaga
      121 tcagaaaacc attatctttc gttttatttt tatctcacca tgacgcagta tcaactgaaa
      181 caaaacgaaa gattaatgtc gcagtaatct gaactggaga ggaaagtgaa acatctgaca
      241 gaaatggtga gacagcacaa agcgggcaaa acaaatggga tttatgccgt ttgttccgca
      301 catccgctgg tgctggaagc tgcaatccgc tacgccagtg caaaccaaac gccgttactg
      361 attgaagcaa cctccaatca ggtggaccag ttcggcggtt ataccggaat gacgcccgcc
      421 gattttcgcg gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgctg
      481 attctgggtg gtgaccatct ggggccaaac cgctggcaaa acctgcctgc cgctcaggca
      541 atggccaatg ccgatgattt ggctaaaagc tacgtagcgg caggattcaa aaaaattcac
      601 cttgactgca gcatgtcctg tcaggacgat ccgattccct taaccgatgg catcgtggct
      661 gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct gtcgtgaaca ctttggcgaa
      721 gccgatctgg agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc
      781 ttaagcgagc tggcggtcac cacgccggat gccgcccgcg ccacgctgga agcccatcgt
      841 cacgcctttg aaaagcaagg tctgaatgcc atctggccac gcatcattgc cctggtggtt
      901 caacccggcg tcgaattcga tcacaccaac gttattgatt atcagccagc caaagccgct
      961 gccttaagcc agatggtcga aaactacgaa acgctgattt tcgaagcgca ctctaccgat
     1021 tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc actttgccat tctgaaagtt
     1081 ggcccggcgc tgaccttcgc cctgcgtgaa gctctgttct ctctggcggc gattgaagaa
     1141 gaactggtgc cagcgaaagc ctgttccggt ctgcgtcagg tactggaaaa cgtgatgctc
     1201 gaccgcccgg aatactggca aagccactac cacggtgacg gcaacgcgcg tcgtctggcg
     1261 cgtggttata gctactcgga tcgcgtgcgc tattactggc cggacagcca gattgatgac
     1321 gctttcgctc atctgattcg taatctggcg gattcaccaa ttccgctgcc gctgatcagt
     1381 cagtatctgc cgctgcagta cgtgaaagtt cgctccggcg agctgcagcc aacgccacgg
     1441 gaactcatta tcaaccatat tcaggacatc ctggcgcagt accacacagc ctgtgaaggc
     1501 caataagcaa aacaaagagg aacacgctat gccaaatatt gttttaagtc ggattgatga
     1561 acgcttgatt cacggtcagg tcggcgttca gtgggtcgga tttgcggggg caaatctggt
     1621 gctggtagcc aacgatgagg ttgccgaaga tccggtacaa caaaacctga tggaaatggt
     1681 actggcagaa gggatcgccg tacgtttctg gacgctgcaa aaagtcatcg acaacattca
     1741 tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa acaccggccg atttcctgac
     1801 gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa
     1861 tggcaagcaa caaatcgcca aaacggtttc tgtggatgcg ggtgatatcg cagcatttaa
     1921 cgacctgaan nnnnnnnnna ctgatcgcct ttttcgctgg actggatagt tttaacggct
     1981 taacccatat gcaccgcccg gtggttctcg gcccgttggt cgggctggta cttggcgatc
     2041 tgcataccgg tattttaacc ggcggtacgc tggaactggt gtggatgggg ctggcccctc
     2101 tggcgggcgc acagccgctt aacgtaatta tcggtactat cgtcggcacg gcgtttgcca
     2161 ttactactgg cgtgaaaccc gatgtcgcgg tcggtgtcgc cgtacctttc gctgtcgcag
     2221 tacagatggg gattaccttc ctgttctcgg tgatgtccgg cgtgatgtcc cgatgcgacc
     2281 ggatggcgga gaacgccgat acacgcggca ttgaacgcgt gaactatctg gcattgctgg
     2341 cactcggcac gttctatttt ctctgcgctt tcctgcctat ctacttcggc gcggaacatg
     2401 caaaaaccat cattgatgtc ctgccgcaac gattaattga tggcctcggc gtcgcgggcg
     2461 gcatnnnnnn nnnngggctt tgttgccgcg gcctggctca agttaccggt gctggctatt
     2521 gctgccgctg cgctggcgat ggcgctgatc gacctgctgc gtaaatctcc tgaaccgact
     2581 caacctgcgg cacagaaaga ggaatttgaa gatggcatct aatcaaacca ccctgccgaa
     2641 cgtcagcgaa aatgaagaaa cactgctgac tggcgtcaat gaaaacgtgt atgaagatca
     2701 gagcattggc gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct
     2761 gttacaggct tctttcaact acgaacgtat gcaggcttcc ggctggctgt acggcctgct
     2821 gccagcactg aaaaagatcc acactaataa acgcgacctg gcgcgcgcca tgaaggggca
     2881 tatgggtttc ttcaataccc atccgtttct ggtgacgttt gttatcggca ttatccttgc
     2941 gatggagcgt tctaagcagg acgttaacag tattcagagc accaaaattg ccgtcggtgc
     3001 gccgctcggc gggattggcg atgcgatgtt ctggctaacg ctactgccga tttgtggcgg
     3061 gataggtgca agcctcgcgc tacaaggctc tattcttggc gctgtcgtct ttattgtgct
     3121 gttcaacgtg gtgcacctgg ggctgcgttt tggtctggcg cattatgctt accgcatggg
     3181 cgtggcggcg attccactca ttaaagcaaa taccaaaaaa gtcggccatg cggcatctat
     3241 cgttgggatg acggtaatcg gcgcgctggt ggcaacctat gttcgtttaa gcaccacgct
     3301 ggaaatcacc gcgggcgacg cagtggttaa gttacaggct gatgttatcg acaaactgat
     3361 gccagccttc ttaccgctgg tctacaccct gaccatgttc tggctggtac gccgcggctg
     3421 gagtccgctg cgcctgattg cagtgacagt ggttctcggc atcgtcggta aattctgcca
     3481 tttcctttaa atacaatgag gtattagatg ttaagtatta ttttgacagg gcatggcgga
     3541 tttgccagcg gcatggaaaa agcaatgaag caaattctcg gtgagcaatc gcagtttctc
     3601 gccatcgatt ttccggaaac ctcaagcaca gcgctgctta catcgcagct tgaagaggcc
     3661 atcgcccaac tggattgtga agacggcatt gtttttctga cggatttgct gggcggcacg
     3721 ccgtttcgcg tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc
     3781 acaaatttgc aactattact ggaaatggtg ctggaacgcg aagggttaag cggtgaagag
     3841 ttccgtgtgc aggcgctgga gtgtggacac cgtgggctaa ccagtctggt ggacgaactt
     3901 ggtcgctgcc atgaggaatg tccggtcgag gaaggaatat gacacacgtt ctgcgcgcca
     3961 gaaggctgct gactgaagag ggatggctcg atgaccatca gttgcgtatt gctgacggtg
     4021 taatcgcagc aatcgaaccg attccagtgg gcgtgactga acgcgatgcg gaactgctct
     4081 gccccgctta catcgacacc catgtacacg gtggtgcggg cgttgatgta atggatgacg
     4141 cgccggatgc gctcgacaag ctggcaatgc acaaagcacg cgaaggtgtc ggcagttggt
     4201 taccgaccac cgtaaccgcg ccgcttaata ccattcatgc ggcgctggaa cgtattgctc
     4261 aacgttgcca acgcggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt
     4321 acttcacgcc gcagaataaa ggcgcgcatc caccggagct gttccgcgag ctggaaattg
     4381 c
//
LOCUS       DAWDRC010000334         4381 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8441_length_4381_cov_2.150254, whole genome shotgun sequence.
ACCESSION   DAWDRC010000334 DAWDRC010000000
VERSION     DAWDRC010000334.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8441_length_4381_cov_2.150254"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1323..1332
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2634..2643
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1082 a          990 c         1229 g         1060 t
ORIGIN      
        1 aataaaatgc gtactttact gaccatgctg gggattatta tcggtattgc gtcggtggtt
       61 tccattgtcg tggtgggcga cgccgccaaa caaatggtgc tggcggatat tcgttctatt
      121 ggtacgaata ctattgatgt ctatcccggg aaagattttg gcgatgacga tccgcaatat
      181 cagcaggcgc tgaagtacga cgacttaatc gccatccaaa aacaaccgtg ggttgcctct
      241 gccacgcccg ccgtctcgca aaacctgcgc ctgcgttata acaatgttga tgtggctgcc
      301 agtgccaatg gtgtgagcgg cgattatttt aatgtctatg gcatgacctt cagtgaaggg
      361 aacaccttta atcaggagca gctgaacggt cgtgcgcagg tcgtggttct cgacagtaat
      421 actcgccgcc agcttttccc ccataaagca gatgtggttg gcgaggtgat tctggtcggc
      481 aatatgcccg ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt
      541 aaagtgctgc gtgtctggct gccatacagc acgatgtccg ggcgagttat ggggcagtcg
      601 tggctgaact ccattactgt cagggtgaaa gaaggctttg acagcgctga ggcggaacag
      661 caactcacgc gtttactttc actgcgccac ggtaagaagg atttctttac ctggaacatg
      721 gatggcgtct tgaaaaccgt tgaaaagacc acacgtactt tacaactgtt tctgacgctg
      781 gtggcggtga tttcgctggt ggtgggcggt attggtgtaa tgaatattat gctggtgtca
      841 gtgaccgagc ggacgcggga aattggcatt cgcatggctg taggtgcgcg agcaagcgat
      901 gttttgcaac agtttttgat cgaagccgta ctggtttgcc tggttggtgg cgcgttggga
      961 ataacactgt cactgttaat tgctttcacc ttgcagcttt tcttacccgg ctgggagatt
     1021 ggtttttcac cgttagcgct gctgctggcg tttctctgct cgacggtcac cgggatttta
     1081 tttggctggt tacccgcacg aaatgcggca cgactggatc cagtagatgc tctggcacga
     1141 gagtaatttt tgagataaaa atgccagccg atcgggctgg cattttgcct ttaggatgta
     1201 cacaatgaga cagaagagct atgcgactgc cgcttctact tcgacgggca caataacact
     1261 ggcgtgattg ccttttggcc cctggtggac atcaaactga acggattgtc cggcttttag
     1321 cgnnnnnnnn nnttctgtaa ccatccatct gaatggtaga ataatgagcg aaaatatctt
     1381 cgccgccgcc ttcagggcag atgaaaccaa accctttggc attgttgaac cacttaacag
     1441 tacccttttc catgcttcga catccttcgc aaatcttata caagtaagat ggaataaacc
     1501 ggggtcagag agggggctgt tcaaaacctc gccaactcta gaaatacaat ttagagaatt
     1561 agggcgagcc gtcaagcatt tgacaggggc cagggggcag gtatgaatca aaaatttgaa
     1621 gcagttaacg ctattgacag gaatgtgaca gatgtcgctg atgccaacga tagatgatag
     1681 ttatctatca tgtggagtag attggtcagg caaataagct cttgtcagcg gcagggcgtt
     1741 ctgccgataa ccgtaaccga agatgataac tgacaatggg taaaacgaac gactggctgg
     1801 actttgatca actggcggaa gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata
     1861 aagtgatatt agtcaatgat gattatactc cgatggagtt tgttattgac gtgttacaaa
     1921 aattcttttc ttatgatgta gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg
     1981 ggaaggccat ttgcggagtc tttaccgctg aggttgcaga aaccaaagtg gcgatggtga
     2041 acaagtacgc gagggagaat gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc
     2101 aggcataaaa attgggggag gtgcctatgc tcaatcaaga actggaactc agtttaaata
     2161 tggctttcgc cagagcgcgc gagcaccgtc atgagtttat gaccgtcgag cacttgttac
     2221 tggcgctgct cagtaaccca tctgcccggg aggcgctgga agcgtgttct gtggatttgg
     2281 ttgcgctccg tcaggaactg gaagccttta ttgaacaaac cacacccgtt ctgcctgcca
     2341 gtgaagagga gcgcgacaca cagccgacgc tgagttttca gcgtgtactg caacgtgcgg
     2401 tcttccatgt ccagtcctcc ggtcgcaatg aggttaccgg tgcaaacgtt ctggtcgcta
     2461 tctttagcga acaggagtcg caggcggcat atctgttgcg caaacatgaa gtcagccgtc
     2521 tcgatgtggt gaactttatc tctcatggca cgcgtaaaga cgagccgaca cagtcttctg
     2581 atcctggcag ccagccaaac agcgaagaac aagctggtgg ggaggaacgt atgnnnnnnn
     2641 nnnagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa caacccgctg ctggtagggg
     2701 agtctggtgt cggtaaaacc gcgattgcgg aggggcttgc atggcgaatt gttcagggcg
     2761 atgtgccgga agtgatggct gactgtacga tttactctct cgatatcggt tctctgttag
     2821 cgggcacaaa atatcgcggc gactttgaaa aacgttttaa agcgttgctc aagcagctgg
     2881 agcaggacac taacagcatc ctgtttattg atgagatcca caccattatc ggtgcgggtg
     2941 cagcgtctgg tggccaggtc gatgcggcta acctgattaa accgttgctc tccagcggta
     3001 aaatccgcgt aattggttcg acaacctatc aggagttcag caacattttc gagaaagacc
     3061 gtgctctggc gcgtcgcttc cagaaaattg atattactga accgtcaatc gaagaaactg
     3121 ttcaaatcat caatggcctg aaaccgaagt atgaagcgca ccacgacgtg cgttataccg
     3181 caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata cattaacgat cgtcatctgc
     3241 cggataaagc gattgatgtt atcgacgaag cgggcgctcg cgcacgtctg atgccggtaa
     3301 gcaaacgcaa gaaaaccgtt aatgtggcgg atattgaatc cgtggtggcc cgtattgcac
     3361 gcattccaga gaagagtgtt tctcagagtg accgcgatac cctgaaaaac ctcggcgatc
     3421 gcctgaaaat gctggtcttc ggtcaggata aagccattga ggcgctgact gaagccatta
     3481 agatggcgcg tgcaggttta ggtcacgaac ataaaccggt cggttcgttc ctgtttgccg
     3541 gccctactgg ggtcgggaaa acagaggtga cggtacagct ttcgaaagcg ttgggcattg
     3601 agcttctgcg ctttgatatg tctgagtata tggaacgcca taccgtcagc cgtctgattg
     3661 gtgcgcctcc gggatacgtt ggttttgatc agggcggttt gctgactgat gcggtcatca
     3721 agcatccaca tgcggtgctg ctgctggacg aaatcgagaa agcgcacccg gacgtgttca
     3781 acatcctctt gcaggtgatg gataacggta cgctgaccga taacaacgga cgcaaagcgg
     3841 acttccgtaa cgtggtgctg gtgatgacca ctaacgcggg ggtacgtgaa accgagcgca
     3901 aatccattgg tcttatccac caggataaca gcaccgatgc gatggaagag atcaagaaga
     3961 tctttacacc ggaattccgt aaccgtctcg acaacattat ctggttcgat catctgtcaa
     4021 ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga gttgcaggtt cagttggatc
     4081 agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa ctggctggcc gagaaaggtt
     4141 acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat ccaggacaac ctgaaaaaac
     4201 cgctcgccaa cgaactgctg tttggttcgc tggtggacgg cggtcaggtc accgtcgcgc
     4261 tggataaaga gaaaaatgag ctgacttacg gattccagag tgcacaaaag cacaaggcgg
     4321 aagcagcgca ttaatctgat tgtcaggtag gttggttaag tccgtgatct cgtcaggggt
     4381 t
//
LOCUS       DAWDRC010000335         4373 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8459_length_4373_cov_2.027791, whole genome shotgun sequence.
ACCESSION   DAWDRC010000335 DAWDRC010000000
VERSION     DAWDRC010000335.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8459_length_4373_cov_2.027791"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1005 a         1143 c         1141 g         1084 t
ORIGIN      
        1 ctggcgtcag gacgccgctg aattttattt ctcacgctta agccacctgc cgtgggcgat
       61 gcttttacac tccggctatg ccgatcatcc atatagccgc tttgatattg tggtcgccga
      121 gccgatttgc actttaacca ctttcggtaa agaaaccgtt gttagtgaaa gcgaaaaacg
      181 cacaacgacc actgatgacc cgctacaggt gctccagcag gtgctggatc gcgcagacat
      241 tcgcccagcg cataacgaag atttgccatt tcagggcggc gcgctggggt tgtttggcta
      301 cgatctgggc cgccgttttg agtcactgcc agaaattgcg cagcaagata tcgttctgcc
      361 ggatatggca gtgggtatct acgactgggc gctggttgtc gaccaccagc gtcaaacagt
      421 ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc tggctggaaa gccagcaatt
      481 ctcgccgcag gaagatttca cgctcacttc cgactggcaa tccaatatga cccgcgagca
      541 gtacggcgaa aaatttcgcc aggtacagga atatctgcac agcggtgatt gctatcaggt
      601 gaatctcgcc cagcgttttc atgcgaccta ttctggcgat gaatggcagg cattccttca
      661 gcttaatcag gccaaccgcg cgccgtttag cgctttttta cgccttgagc agggcgcgat
      721 tttaagcctt tcaccagagc ggtttatcct ttgtgataat agtgaaatcc agacccgccc
      781 gattaaaggc acgctaccac gcctgcccga tcctcaggaa gatagcaaac aagcagagaa
      841 actggcgaac tcagcgaaag atcgtgccga aaatctgatg attgtcgatt taatgcgtaa
      901 tgatatcggt cgtgttgccg tagcaggttc ggtaaaagta ccagagctgt tcgtggtgga
      961 acccttccct gccgtgcatc atctggtcag caccataacg gcgcaactac cagaacagtt
     1021 acacgccagc gatctgctgc gcgcggcttt tcctggtggc tcaataaccg gggctccgaa
     1081 agtacgggct atggaaatta tcgacgaact ggaaccgcag cgacgcaatg cctggtgcgg
     1141 cagcattggc tatttgagct tttgcggcaa catggacacc agcattacta tccgcacgct
     1201 gactaccatt aacggacaaa tttactgctc tgcgggcggt ggaattgttg ccgatagcca
     1261 ggaagaagcg gaatatcagg aaacttttga taaagttaat aagatattac gccaactgga
     1321 gaagtaagac gtggaatacc gtagcctgac gcttgatgat tttttatcgc gctttcaact
     1381 tttgcgcccg caaattaacc gggaaacctt aaatcatcgt caggctgccg tgttaatccc
     1441 catcgtccgc cgaccgcaac cggggttgtt gctgactcag cgttcgattc atctgcgtaa
     1501 acacgctgga caagtggcat tccctggagg cgcagtcgat gacacggacg catcagttat
     1561 cgccgctgcg ctgcgcgaag ctgaagaaga ggtcgctata ccgccttccg ccgttgaggt
     1621 tatcggcgtg ctgccgcccg tcgatagcgt cactggctat caggtaaccc cagtggtcgg
     1681 cattatcccg cccgatctgc cgtatcgcgc cagtgaagat gaagtctccg ccgtctttga
     1741 aatgccgctc gcccaggcat tacatctggg tcgttatcat cctttagata tctaccgccg
     1801 tggcgattca catcgggtat ggctgtcctg gtacgaacag tattttgtat ggggaatgac
     1861 cgcaggcata attcgtgagc tggcgctgca aattggtgtg aaaccctgac tatacttatc
     1921 tttacatcta caaaacacta cttgagacaa tcatcgcaat attagttaaa tcgcggtttt
     1981 tgattagttt aattcatgtg aatagttaag ccagtcgccg cgttccctct tacactatgc
     2041 gctgttatta gttcgttact ggaagtccag tcaccttgtc aggagtatta tcgtgattag
     2101 tctattcgac atgtttaagg tggggattgg tccctcatct tcccataccg tagggcctat
     2161 gaaggcgggt aaacagttcg tcgatgatct ggtcgaaaaa ggcttactgg atagcgttac
     2221 tcgcgttgcc gtggacgttt atggttcact gtcgctgacg ggtaaaggcc accacaccga
     2281 tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc accgtggata tcgacagtat
     2341 tcccggtttt attcgcgacg tagaagagcg cgaacgtctg ctgctggcac agggacggca
     2401 tgaagtggat ttcccgcgcg acaacgggat gcgttttcat aacggcaacc tgccgctgca
     2461 tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa gtcgtctaca gcaaaactta
     2521 ttattccatc ggcggcggtt ttatcgtcga tgaagaacac tttggtcagg atgccgccaa
     2581 cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa ctgctcgcgt actgtaatga
     2641 aaccggctat tcgctgtctg gtctcgctat gcagaacgaa ctggcgctgc acagcaagaa
     2701 agagatcgac gagtatttcg ctcatgtctg gcaaaccatg caggcatgta tcgatcgcgg
     2761 gatgaacacc gaaggcgtac tgccaggccc gctgcgcgtg ccgcgtcgtg cgtctgccct
     2821 gcgccggatg ctggtttcca gcgataaact gtctaacgat ccaatgaatg tcattgactg
     2881 ggtaaacatg tttgcgctgg cagttaacga agagaacgcc gccggtggtc gcgtggtaac
     2941 tgcgccaacc aacggtgctt gtggcattgt tccggcagtg ctggcttact atgaccactt
     3001 tattgagtcc gtcagcccgg acatctatac ccgctacttt atggcagcgg gtgcgattgg
     3061 tgcactgtat aaaatgaacg cctctatttc cggtgcggaa gtcggttgcc agggcgaagt
     3121 gggtgttgcc tgttcaatgg ctgctgcggg ccttgccgag ctgttgggcg gtagcccgga
     3181 acaggtttgt gtggcggcgg aaattggcat ggagcacaac ctcggtctga cctgcgaccc
     3241 ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat gctattgcct ctgtgaaagc
     3301 gatcaatgct gcacgcatgg ccttacgccg caccagtgca ccgcgcgtct cgctggataa
     3361 ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac gctaaatacc gcgaaacctc
     3421 acgcggtggt ctggcaatca aagtccagtg tgactaatac ttcttactcg ctcatctgca
     3481 acggatgggc gaatttatcc ccgctttctc gtctgctgta attttcccca ctacacttcc
     3541 actgttgcgt caggcgtttg tcgccatacg cttacagggt ggcccgcatg caaaaagcac
     3601 aacggatcat taaaacctat cgccgtaatc gaatgattgt ttgtacgatt tgcgcactcg
     3661 ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca gcgtaactta aatcaacaac
     3721 gggtagtaca attcgccaat cacgctgtag aggaattaga taaagtactg cttcccctac
     3781 aggcaggtag cgaagtcttg cttccgctga ttggtctgcc ctgctctgtc gcccatttgc
     3841 cattacgtaa acaggctgca aaactccaaa ctgtgcgatc cattggcctg gtgcaagacg
     3901 gcacacttta ttgctccagc atttttggtt atcgcaatgt gcccgtcgtg gacattctgg
     3961 ctgaacttcc tgcaccgcaa ccacttttac gactgacgac cgaccgtgcc ctgataaaag
     4021 gcagtccggt tttgattcaa tggacgcctg cagcgggcag tagcaatgct ggggtcatgg
     4081 agatgattaa catcgactta ctgacggcaa tgctccttga accacaactg ccgcaaatca
     4141 gtagcgccag cctgacggtg gacagtcggc atttgctcta tggtaatggg ctggtagatt
     4201 cccttccgca acctgaaaac aatgaaaact atcaggtttc ttcgcaacgc tttcctttta
     4261 ccattaacgt taatggccca ggggctacgg cgctggcatg gcactatctt ccaacacaat
     4321 taccgctggc ggtgctgcta agtttactgg tgggctacat cgcctggctg gcg
//
LOCUS       DAWDRC010000336         4370 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8468_length_4370_cov_2.317729, whole genome shotgun sequence.
ACCESSION   DAWDRC010000336 DAWDRC010000000
VERSION     DAWDRC010000336.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_4370_cov_2.317729"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    920..929
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2131..2140
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1085 a         1140 c         1105 g         1020 t
ORIGIN      
        1 gagcggcagc accgtcgtta atgccagacg cgttcccggc ggtgactgtt cctgctttat
       61 cgaaggccgg gcgcaatgcg cctaacgctt cagccgtaga atccactttc gggaattcgt
      121 cttgactgaa gacgagggtt ttcttccggg tgacaatatt taccgggacg atttcggctg
      181 taaaagcacc ggactcaatt gcggctgcag ctttacgctg tgaatgtagc gccagttcat
      241 cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta atccccatat
      301 gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa acctgtccgt
      361 ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct aaactcatat
      421 tttccatacc ccccgccaca atgctctgtg cctgacctgc ctgaatcgcc tgtgcagcaa
      481 gcgccacgct tttcagacct gaaccgcaca ctttgttgac cgtgaatccg cacaccgttt
      541 cagccagtcc gctttttagc agcgcctgac gtgccggatt ttgccccagt ccggcttgca
      601 acacgttacc cataatcact tcatcaacgt gtagtgaatc gatttttgca cgttcaatgg
      661 cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggacgcgagt gaaccgttaa
      721 aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt ttatattcct
      781 tctgtttgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg acgccggaga
      841 agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg gcgattgcca
      901 gtgctggccg cgcccagaan nnnnnnnnna tccattgctc gccgtaggcg atcgccatta
      961 ccgatttacc gagatcagcg cccagcgcct gggctgcggg catcacgaac ggcccctgaa
     1021 taacccagtg cccgccgcca gacggaacgg cgaagttaat cagtgcagaa ctaaaaaagg
     1081 tcatgaccgg gaaggtgtct ttgttcgcaa cattgatgaa gaattcggta atgagtccgc
     1141 ccagaccgga gtgctccatc atcagttgga tccccgcgta gaaggggaat tgcaccagaa
     1201 taccggcggt actgcgtgct gccgcgctga tagcacgcat ataagccatt ggcgttttat
     1261 gtagcagcag acccgcaatc ataaacatca ggttgacggt attgatggtg atgttgaagc
     1321 catgttcgct gaagtacatc gccaggtaag cgataccgag tgcgccgatg atcaacgtca
     1381 gaatgcggct ttcttccagt cgctcggacg gtggagcatc tttcggtagt tgcttttgaa
     1441 aatcagcctc ttccatgagt agtttcggat cgatactcac cacgtcagac ggttttggca
     1501 tcatcatgcg ggtgataaat ggcatcacca caatcaacgc cacagtgatg aaaatattaa
     1561 aaccactgaa cagagtatcg cccaccggga tcagcccggc gatatgctca accgggttgc
     1621 ccggtgttgc agccaacaga ggcattgagc cagagaaacc gccaccccag gtgagaaaac
     1681 caatgtaggc gcaggcaatg agcaacggat aatcagaacc gggtactcgc cgggcgactt
     1741 cacgggcgaa cattgcaccg acaaccaaac caaatcccca gttgatgaca caagcgactg
     1801 aaccgaagaa agtaaccagc atgacgccct gtacgggcgt ctttgcggca gaggcggcag
     1861 tacgcagcaa acttttcacc ggagcggagc tggcaagggc atgaccggta acgatgataa
     1921 gcgccatctg cataccaaac gccagcaagt tccagaaacc gtcaccccac attttcacca
     1981 tgctgatcgg cgtttgtggt gttaaccaaa gcgcgatcac gaatgttagc aatgtcagca
     2041 acatggcaaa gatcagtgga tcgggaagcc accggctgac aaaacgcgtc ataaaacgcg
     2101 atatgcgacc aatcataaat caccccgttg nnnnnnnnnn agcttctgtt ttggcacgca
     2161 cggtggctaa atcacacccg tcggcaattt cggtgagcca cattttgccg tcaataaaac
     2221 gaaagacagc cagttcagta accagcatat gcaccgcatg ttgcgcagtg agtggcatgg
     2281 tgcagcggcg caaaattttt gctgaaccat ctttggcgca atgttccatg gcgatgatca
     2341 ctttgcgcga cccggtcacc agatccatcg cgccccccat accgggcacc attttcccag
     2401 gcactaccca gttcgcgagg tttgcttctt cgtctacttg caaaccgccg agcacgcagg
     2461 catcaatatg accgccacgg attagcgcaa atgacatggc gctatcaaac atggctgcac
     2521 cgggtaaaat gccgcacggt tgtccgccag cgttcaccag atcgggatgc gctgttgtga
     2581 ccgggcctaa accgaggaag ccgttttccg attgcagagt gatatgaata ccctctggta
     2641 aataattggc gaccattgtg ggtaaaccga tccctaagtt aacgatgtca ccatcacgaa
     2701 gctcttgcgc cacacggcgc gcaatacgtt gtttcgcatc cattatttgc tctcctgtga
     2761 aacgatgatg tggtcgataa cggcaccagg ggtgacaata tggtcaggtt gcagctcgcc
     2821 ggtttcgacc agttcatccg gctctaccag cgtgatatca gccgcaaggg ctatcagggg
     2881 attaaagttg cgggcgctaa ggtgataggt caggttgcca agtgtgtcgc aacgatgagc
     2941 gcgaattagc gccaggtcgg cgcgcagcgg gcgttcgagc agccaggttt taccgtcgag
     3001 tgtcagtgtc tgtttgcctt cctctacgac ggtgccgaca cccgttgggg tgagaaaacc
     3061 accaagtcca gctccgccac agcgaatttg ctcgattagc gtaccttgcg gcaccagaac
     3121 gacgtccatc tcaccagata tcatgcgccg acctgtttcc gggttggtgc cgatatgtga
     3181 agcaatcact ttgcggactc gaccattgac gatgagcgga ccgatgccgg tatcaacaaa
     3241 cgcggtatca ttggctatca atgtcaggtc gcgaacacca gattccagta atgcttcaac
     3301 caggcgggat ggagtgccaa tccccataaa tccgcccacc atgatggtca tgccgtcacg
     3361 aaagaagccg gtggcgtctt gtaatgtcat caattttgtt ttcatttttt atccctcttg
     3421 catacaccgt tgtgggtaca gtcagggaat agcaagggga gtgccagaat gagattattg
     3481 aatgttgtta tttaactaat tgaatagaaa aggataaatt agatggggat tcataaggag
     3541 aatgagcggc tatgcagaaa attgcgcact gtgcaaattt ctgcatagca agttttggcg
     3601 ttatacatcc gccggatcga taccgtattc ctggagttta tacatcaatg cacggcgact
     3661 gatgcccagc attaaagcag tgcgggttcg gtttccttct tgttgttcca gcacttccat
     3721 aatgatgcgt ttttcgacgc gtttaatttc ctcttttaaa ttacgctcac cgacaggggc
     3781 tgtttttgct tcaccagcat tacagactgg ctgacgaatc tgcggcggaa gatcctcaga
     3841 aaaaatgatc gggcctgaat tcatcacgac ggcgcgttca ataacgttgg aaagctctcg
     3901 aatatttcct ggccatgacc aggcggtaag cagtgacatt gccatcggat cgatgtcgat
     3961 aatatcgcgc tgattctcac tactgaattt ttgcaaaaag tgattagcta acagggaaat
     4021 atcttcccgg cgatcgcgca gaggcggcag tattaaatga ataacgttaa ggcgataaaa
     4081 gagatcttca cggaaggtgc cttctttcac cattgcctgc aagtcgcggt tggtggcagc
     4141 aatgatgcgg atatcaactt ttatggtctg atggccgcca atccgttcaa attcccgttc
     4201 ctgcagaatg cgtagtaatt tggcttgcag taccagcggc atttcgccaa tttcgtcgag
     4261 gagcagagta ccttcgttgg cacgttcaaa taatccctga cgcaaggttt gtgcaccagt
     4321 aaatgcacct ttttcatagc caaataattc actctcaaac aattgttctg
//
LOCUS       DAWDRC010000337         4367 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8473_length_4367_cov_2.611781, whole genome shotgun sequence.
ACCESSION   DAWDRC010000337 DAWDRC010000000
VERSION     DAWDRC010000337.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8473_length_4367_cov_2.611781"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1082..1091
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1132 a         1076 c         1067 g         1082 t
ORIGIN      
        1 gttatataag gagatatatc catgtttgat aaatatagaa aaacactcgt agccggaact
       61 gtggcgataa ccctgggttt gtcagcatcg ggggtgatgg ctgcgggttt taaaccagcg
      121 ccgcctgccg ggcagcttgg ggcgattatc gtcgatccct acggtaatgc gcccttaacc
      181 gcgctggttg atttagacag ccatgttatt tctgacgtca aagttaccgt ccacgggaag
      241 ggcgaaaaag gcgttgacat cagctataaa gtgggcaatg agtcactgaa aacttacgat
      301 ggtgtaccga tttttggtct ttaccagaaa tttgcgaaca aagtgaccgt tgagtggaaa
      361 gaaaacggca aggccatgaa agatgattac gtggtgcaga cttccgccat cgtcaacaat
      421 tacatggata accgctccat ctccgaccta cagcaaacca aagtcgttaa agtcgcgcca
      481 ggttttgaag atcgcctcta tctggttaat acccacacct ttaccgccca aggttccgat
      541 ctccactggc atggtgagaa agataaaaat gccggtatcc ttgatgcggg tccggcaact
      601 ggcgcactcc cttttgatat cgcgccattc acctttatcg tcgataccga aggtgaatac
      661 cgctggtggc tggatcaaga cactttctac gatggtcgtg accgcgatat caacaagcgt
      721 ggttatctga tgggtatccg ctgtacaagg tcagcactgg tacgagttcg acatgatggg
      781 acaggtgctc gaagatcaca aactaccgcg cggatttgct gacgccactc atgagtccat
      841 tgagacgcca aatggcacgg tactgttgcg cgtaggtaag agtaactatc gtcgcgatga
      901 cggcgtacac gtcaccacca ttcgtgacca tatcctcgaa gtcgataaat ctggtcgcgt
      961 tgtcgatgta tgggatctga ccaagatcct cgatccgaaa cgcgatgcac tgctcggcgc
     1021 gctggatgca ggtgcagttt gcgttaacgt tgaccttgcc catgcaggac aacaggcaaa
     1081 annnnnnnnn nccagataca ccgttcggcg acgcgctggg tgtagggcca ggccgcaact
     1141 gggcgcacgt gaactctatc gcttatgacg caaaagatga ctctatcatc ctctcctctc
     1201 gccaccaggg tgttgtgaag attggtcgtg ataagcaagt gaaatggatc cttgcgccct
     1261 ctaaaggttg ggaaaaaccg ctggccagca agctgctgaa accggtggac gctaacggta
     1321 aacctattac ctgtaacgaa aacggtctgt gtgaaaactc ggacttcgat tttacctaca
     1381 cccagcatac cgcctgggtt tccagcaaag gaacgctcac catttttgat aatggcgatg
     1441 gtcgtcatct ggaacaacct gccttaccaa ccatgaaata ttcccgcttt gtggaatata
     1501 agattgatga gaagaaaggc accgttcagc aagtgtggga atacggtaaa gaacgtggct
     1561 acgatttcta tagcccgatt acctccatca ttgaatatca agccgaccgt aacaccatgt
     1621 ttggcttcgg tggttctatt catttgttcg atgtcgggca gccaaccgtc ggtaagttga
     1681 acgaaatcga ttacaaaacc aaagaagtga aagtggaaat cgacgtgctg tcagataaac
     1741 ccaatcagac tcactaccgt gcgctgttag tccgtccaca acagatgttc aaataattaa
     1801 gaagggaata aaaatgtcga aattagggat ctcatcacta tttaaaacca tactgcttac
     1861 cgcagctttg gctgtttcat ttaccgcttc tgcatttacc gaaggcacgg attacatggt
     1921 tctggaaaaa cctattccga atgccgacaa aacgctgatt aaggtattta gctacgcctg
     1981 cccgttctgt tacaagtacg acaaagcggt taccggtccg gtatcggaaa aagtgaaaga
     2041 tatcgttgct ttcacgccgt tccatctgga aacaaaaggt gaatacggca aacaagcaag
     2101 cgaagtcttt gcggtcttga tcaataaaga taaagcagca ggcatttcct tatttgatgc
     2161 taactctcag tttaagaaag ccaagtttgc ctactacgcg gcttatcacg acaaaaaaga
     2221 acgctggtct gacggtaaag atcccgctgc ctttatcaaa actggtctgg atgccgcagg
     2281 tatgagtcag gcggattttg aagcggcact gaaagaacca gcggttcaag aaacactgga
     2341 aaaatggaaa gcctcttatg atgtcgccaa aatccagggg gttccagcct atgtcgttaa
     2401 tggtaagtac ttgatctaca ccaagagcat caaatccatc gacgcgatgg cagaccttat
     2461 ccgtgagtta gcaagcaaat aacagggaaa caggctatgg gtattaaggg aatgtggaaa
     2521 gatcttcgca catcaccagt tgacacactg gtcagatggc aggagcaacg cttgttgtgg
     2581 ttgttaatgg ccgttgcgat gggcgcgtta attatcctgg cgcactcttt cttccagatc
     2641 tatctctaca tggcaccctg tgagcaatgt gtatacattc gctacgccat gtttgtgatg
     2701 gttattggag gattggttgc agcgatcaac ccaaagaaca tcatcctaaa gctaattggc
     2761 tgcgtgatgg cattttacgg cagcattttg ggactgaagt tttcgctcaa actgaacgat
     2821 atccaccatg cagtacataa tccggatccc gattcactgt ttggcgtaca gggttgctct
     2881 accgatccaa cttttccctt taacctgccg ctggcacagt gggcaccaaa ttggttcaag
     2941 cctaccgggg attgtggcta tgacgcacca atcgttcctg atggcgtcac actgagcagc
     3001 acgcaacagt ggtttgtgga aatgtatcag caatctgaag gctggtatct gctaccacca
     3061 tggcatttta tgaatatggc tcaggcttgt atgctggctt ttggtatgtg tctggtgctg
     3121 ctggtcatta tgagtggcgc atgggcgctt aaaatcatca gaggctaact cagcaacacc
     3181 cgaagccagt cattctggct tcgggttctt ttattaacct atctgcaccg acagcatact
     3241 taaactgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc catcccacgc
     3301 ccctaagaca tacaacaacg gcagatagtg ctctggcgtt gggttcgata acgcgccacc
     3361 ttcatggtcg agataattca ccagaggatg ttgttccacc ggcccttgcc acgtcagatt
     3421 cgctttcaca tactcattaa acgacgtcgc ccacggatac ggtgaactat caccgtgcca
     3481 cttcactgta cgcaggttat gtaccacgtt gccactggcg accaacatta tcccttcatc
     3541 acgcagcgcc gcgagtttgc gccccatttc gaaatgccag gcggcaggtt tgctactgtc
     3601 gatactcaac tgcaccatcg ggatatcggc gtcagggtac atcttaatca gtacgcccca
     3661 cgagccgtgg tcgaagcccc aggcttcttt atccagcgtc acagggaccg gcgctaacag
     3721 ctcaaccaga tgctgtgcca gcgcaggtga acccggagca ggataatacg tatcgtacag
     3781 cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc atctccatcg cggtcactcc
     3841 tgttccacgg gtaaaccaat gagccgaaac caccactatt gcttgcgggc gtggcaatgt
     3901 catccccaac gtctgccagc tgcgggtata caaattatct tccagcacgt tcatcggact
     3961 accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat ccttaactaa
     4021 aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac ccggataaca
     4081 atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtaaagattt ataaggagtt
     4141 gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt ttattggcgc
     4201 gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt cgctaggatt
     4261 tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg cattagccgc
     4321 cgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtc
//
LOCUS       DAWDRC010000338         4359 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8490_length_4359_cov_2.269052, whole genome shotgun sequence.
ACCESSION   DAWDRC010000338 DAWDRC010000000
VERSION     DAWDRC010000338.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8490_length_4359_cov_2.269052"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1695..1704
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1088 a          986 c         1114 g         1161 t
ORIGIN      
        1 tgctcgactc tgtcccggac aacgcgctgg tggtgggaga aaaagcgcga gtgaaggtaa
       61 ttaaatgaat attctgcaat ttaatgtgcg actggcggaa ggcggggcgg caggtgtggc
      121 gttagatctc caccagcgtg cgctgcaaca ggggctggca tcacattttg tctacggcta
      181 cggcaaaggc ggcaaagaga gcgtcagtca tcaaaactat ccgcaggtca tcaaacatac
      241 gccgcggatg accgcaatgg cgaacattgc gttgtttcgt ctgtttaatc gcgatctgtt
      301 cggcaatttc aatgagttat atcgcaccat tactcgcaca ccgggtccgg tggtcctgca
      361 ttttcatgtg ctgcacagct actggttgaa tcttaagagc gtggtgcgct tttgcgaaaa
      421 agtgaaaaac cataaaccgg atatcactct ggtctggacg ttacacgatc actggagcgt
      481 taccggacgc tgcgccttta ccgacggttg tgaaggctgg aaaaagggct gccagaaatg
      541 cccgacctta aataattatc cgccggtgaa gattgatcgc gcacaccagc tggtggcggg
      601 caaacgccag ttattccgtg aggtgctggc gctgggctgt cagtttattt cccccagcca
      661 gcatgtggct gatgctttca atagtctgta cggtccaggg cgttgccgga ttatcaataa
      721 tggcattgat atggcaaccg aagcgattct ggcggacttg cctccggttt gcgaaaccca
      781 gggcaagccg aaaatcgcgg tggtggcgca tgacctgcgt tacgacggca aaactaagca
      841 gcaactggtg cgcgagatga tggcgctggg agacaaaatc gaactgcata cctttggtaa
      901 gttctcgccg ttcaccgctg gtaatgtggt caatcacggc tttgaaaccg acaaacgtaa
      961 actgatgagc gcgctcaacc agatggatgc gctggtattc agttctcgcg tcgataacta
     1021 cccgctgatt ttgtgtgagg cgttatcgat tggcgtgccg gtgattgcca cccatagcga
     1081 tgcggcgcgt gaagtgctgc aaaaatccgg tggtaaaacc gtcagcgaag aagatgtgct
     1141 gcaactggtg cagttaagca aaccggaaat cgcacaggcg atatttggta ccacgctggc
     1201 tgaattcagc cagcgcagcc gcgccgccta cagtggacaa cagatgctgg aggagtatgt
     1261 caacttctat cagaatctgt agctacctgc tgctgccgct gatctacctg ctggttaacg
     1321 tcaaaatcgc ccagcttggc gaaagtttcc ccatcaccat cgtcactttt ttacctgtct
     1381 tgttactgct gtttttagag cgcatcagcg ttaaaaaatt gatgattgcc ttggggattg
     1441 gcgcgggact cacggcgttt aactacctgt ttggtcagtc gctggatgcc agtaaatacg
     1501 tcacttcaac catgctgttt gtctatattg tgatcattat tggcatggtg tggagtattc
     1561 gctttaaaac gatttcgcca cacaaccatc gcaagatatt acgtttcttt tatctggtgg
     1621 tcgggctggt ggtggcgctg gcggcagtgg agatggcgca aattatcctc accggcggta
     1681 gcagtattat ggagnnnnnn nnnntatgga gtcgatttcg aaatatctga tttacagcaa
     1741 cagctatgtg ctgaatttca ttaaattcgg cggcaagcgc acgacagcac tttatttcga
     1801 accggcattt ttcgctctgg cattaatctc aatttggctc agcatcaaac agtttggtat
     1861 caaaacgcct aaaacagatg ctatgattct cgcagggata atattatccg gttcattttc
     1921 aggggttatg acctttatcc ttttttattt gctggagtgg gcatttcaat atctgaataa
     1981 agaggcgatt aagaaaaagt taccgttagc attgatttct ctggctgtat tcctggttgg
     2041 tgtggtaatc gcgttccctt atatttccac ccgtctgggc gatttaggta cagaaggatc
     2101 gtcatcgtat tatcgtattg tcggtccgct ggtgatggtc ggttattctt taacccatat
     2161 tgacggtgta gtcagatttg gctcacttta tgaatatgtc gcatcattcg gaatatttaa
     2221 cggtgcggat gtcggaaaaa ccatagacaa tggtttgtat ctgctgatta tttatttttc
     2281 ctggttcgca gtgtttttat cgctgtggta catggggaaa gtgataaaaa tgatgatcaa
     2341 cgcttttggt gataaccgca attttcgcgt gcaattatat ctttttactc cggtatcgct
     2401 gttttttacc ggttcgatat ttagcccgga atatgcattt ttaatcgtct gtccgtttat
     2461 tttgcgaaaa gcgttaaata ttacgaggta agaataagaa catgttgctt agcataatca
     2521 ctgtcgcgtt tcgtaacctc gcagggatag tcaaaacaca tgcctcgctg gcgcatctgg
     2581 cgcaggcgga cgttatcagc ttcgaatgga ttgttgttga tggcggttcc aacgacggca
     2641 cccgtgagta tctggaaaat ctcaatggta tctataacct acgctttgtc agcgaaccag
     2701 ataacggtat ctacgacgcc atgaacaaag gtattgaaat ggcgcaaggc aagttcgcgc
     2761 tgtttctcaa ttcaggcgat atttttcatc aggatgccgc aaattttgtc cgtaagttaa
     2821 aagtgcaaaa agataacatg atgatcaccg gcgatgcgct gctggatttt ggcgatgggc
     2881 ataaaattaa acgtagcgcc aaaccgggtt ggtatattta ccacagcctg cccgccagtc
     2941 atcaggcgat gtttttcccg gtgtccggct tgaagaaatg gcgttatgac ctggaatata
     3001 aagtttcttc cgcttacgct ctggcggcca aaatgtataa agccggttat gcatttaaaa
     3061 aactcaatgg cctggtgtct gaattttcta tgggtggggt atctaccacc aataatatgg
     3121 aattgtgtgc tgacgccaaa aaagtccaac ggcagatatt gcacgtgcct ggcttttggg
     3181 ctgaattatc ctggcattta cgccaacgta cgacctcaaa gacgaaagcc ttatataaca
     3241 aaagctgaat ataagggaaa acgatgcagg atttaagtgg attctcggtg ccgaaagggt
     3301 tccggggtgg caacgctatt aaagtgcaat tgtggtgggc agtacaggca acaatatttg
     3361 cctggtcgcc acaagtattg tatcgctggc gggcattttt attacgttta ttcggcgcaa
     3421 aaataggaaa aaacgtagtc attcgtccgt cagtaaaaat tacctatccg tggaaattaa
     3481 cgttaggtga ttacgcgtgg gtcggcgatg aggtcaattt atataccctc ggtgaaataa
     3541 ccattggcgc acattcggtg atatcgcaag aaagttattt atgcaccggg agccacgacc
     3601 atgcaagtca acatttcacc attaacgcca cgatcggcga cggcaccgtc gtgggtgcgc
     3661 gaagcagtgt ttttaaatcg cttccggcaa atgtggtttg ccgggggaac cccgcagtgg
     3721 tgatacgcaa acgcgttgaa actgaataaa ttcaaaaaat acagaggaat aagacatgtc
     3781 aaaagtcgct ctcatcaccg gtgtaaccgg acaagacggt tcttacctgg cagagtttct
     3841 gctggaaaaa ggttacgagg tgcatggtat taagcgtcgt gcatcgtcat tcaacaccga
     3901 gcgcgtggat cacatttatc aggatccgca cacctgcaac ccgaaattcc atctgcatta
     3961 tggcgacctg agtgatacct ccaacctgac gcgcattttg cgtgaagtgc agccggatga
     4021 agtgtacaac ctgggcgcaa tgagccacgt tgcggtttct tttgagtcac cggaatatac
     4081 cgcagacgtc gatgcgatgg gtacgctgcg cctgctcgag gcgatccgct tcctcggtct
     4141 ggaaaagaaa acccgtttct atcaggcttc tacttctgaa ctgtacggtc tggtgcagga
     4201 aattccgcag aaagagacca cgccgttcta cccacgctct ccgtatgcgg tcgccaaact
     4261 gtacgcctac tggatcaccg ttaactaccg cgaatcctac ggcatgtacg cctgtaacgg
     4321 tattctcttc aaccatgaat ccccgcgccg tggcgagac
//
LOCUS       DAWDRC010000339         4351 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8510_length_4351_cov_1.738827, whole genome shotgun sequence.
ACCESSION   DAWDRC010000339 DAWDRC010000000
VERSION     DAWDRC010000339.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8510_length_4351_cov_1.738827"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    772..781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2055..2064
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2955..2964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          843 a         1103 c         1254 g         1121 t
ORIGIN      
        1 ttatactgaa gtaataattc ataaatatac aaacaatcaa actataaata taaaatatat
       61 atttttctta ttcaaaatgg atactaacaa ggtattaatg ctgccagcgt ttttaatgat
      121 tagtcgatat accaaacctt gaaggcatca tttcttttca caagcaacag gagcgcagct
      181 tttttcagta accgctcaag gcttgaacag tggcgcgctt atcgttaagg taagcgcgtt
      241 ttattacccg ccaggacaag accatgatca ccgttgccct tatagacgat caccttatcg
      301 tccgctccgg ctttgcgcag ctgctggggc tggaacctga tttgcaggta gttgccgagt
      361 ttggttcggg gcgcgaggcg ctggcggggc tgccggggcg gggtgggcag gggtgtattt
      421 gcgatatctc catgcctgat atctccggtc tggagcttct aagccagctg ccgaaaggca
      481 tggcgacaat tatgctctcc gttcacgaca gtccggcgct ggttgagcag gcgcttaacg
      541 cgggggcgcg cggctttctc tccaaacgct gtagcccgga tgaactcatt gctgcggtgc
      601 atacggttgc cactggcggc tgttatctga cgccggatat tgccattaaa ctggcatctg
      661 gtcgccagga cccgctaacc aaacgcgaac gccaggtggc agaaaaactg gcgcaaggaa
      721 tggcggtgaa agagattgcc gccgaactgg gcttgtcgcc gaaaacggta cnnnnnnnnn
      781 nacgtccatc gcgccaatct gatggaaaaa ctgggcgtca gtaacgacgt tgaactggcg
      841 cgccgcatgt ttgatggctg gtgatgaaga cgttgttctc ccgcttaatt accgttattg
      901 cctgcttttt tatcttctct gccgcatggt tttgcctgtg gagtatcagc ctgcacctgg
      961 ttgagcgccc tgatatggcg gtgctgttat ttccgtttgg cctgcggcta gggctaatgc
     1021 tgcaatgccc gcgcggatac tggccggtgc tactgggtgc ggagtggctg ctgatttact
     1081 ggctaatgca ggcggtcggt ttaacccatt tctccttatt gatgatcggt agtttactga
     1141 cgttattgcc cgtggcgctg atctcgcgct atcgccatca acgtgactgg cgcaccttgc
     1201 tgttacaggg tgcggcgtta acggcggcgg cgttgttgca gtcgctgccc tggctttggc
     1261 acggcaaaga gtcgtggaat gcgctgctgc tgactttaac tggcggcctg acgctggccc
     1321 ccatttgcct ggtgttctgg cactatctcg ccaataacac ctggctgccg ctcggcccgt
     1381 cactggtttc tcagccaatc aactggcgcg ggcgacatct ggtctggtac ttgctgctgt
     1441 ttgttatcag tctctggctc cagttgggat tgccggacga actgtcgcgc tttacgccat
     1501 tctgtctggc gctgccgatt atcgcgctgg cctggcacta tggttggcaa ggagcgctga
     1561 ttgcgacatt gatgaacgcc atcgcgctga tcgccagtca aacctggcgc gatcatccgg
     1621 tggatttatt gctctcgctg ctggtgcaaa gtctgacagg gttgttactg ggcgctggca
     1681 tccagcggtt gcgtgaactt aaccagtcgc tgcaaaagga actggcgcgc aatcagcatc
     1741 tcgctgaacg gttgctggaa accgaagaga gcgtgcgccg tgatgtggca cgtgagctgc
     1801 acgatgatat cggtcagacc attactgcta ttcgaactca agcgggcatt gtccagcggc
     1861 tcgcggcgga taacgccagc gtgaaacaga gcgggcagct catcgaacag ttgtcgttgg
     1921 gcgtttacga cgcggtgcgc cgcttgttag gtcggttacg tccgcgccag ttggatgatc
     1981 tcaccctgga gcaggccatc cgctcactga tgcgggaaat ggagctggag cccaaagcgg
     2041 cccagcggaa gggannnnnn nnnnggaagg gcgcggcatt gtcagccatc tcgaatggcg
     2101 aatcgatgaa tcagcgttaa gcgaaaacca gcgagtgacg ctgtttcgtg tctgccagga
     2161 agggctgaac aacattgtga aacatgctga tgccagcgcg gtcaccctgc aaggctggca
     2221 gcaggatgaa cggttgatgc tggtgattga agacgatggc agcggtttac cgccagattc
     2281 cgggcaacac ggttttggcc ttaccggaat gcgcgagcgc gtaacggcgc tgggtggcac
     2341 attgaccatt tcctgtctgc acggcacgcg tgtcagcgtt tctctacctc aacgttatgt
     2401 ctaaggttcg atgatgttgc cgtttctgaa agcgcccgcc gatgcgccat taatgactga
     2461 taaacatgaa attgatgccc gctatcgcta ctggcgtcgg catattctgc tgaccatctg
     2521 gctgggttac gcgctgtttt acttcacgcg taaaagtttt aacgccgccg taccagaaat
     2581 ccttgctaac ggcgtgctca gccgtagcga tattggcctg ttagcgaccc tgttttacat
     2641 tacctacggc gtgtcgaagt ttgtctccgg cattgtcagc gatcgctcaa atgcccgtta
     2701 ttttatgggg atagggctta ttgccacggg cattatcaac attctgtttg gcttctcgac
     2761 gtcgctatgg gcgtttgccg tgctttgggt gttgaacgcc tttttccagg gctggggttc
     2821 accggtgtgt gcgcgtctgt taaccgcctg gtattctcgt accgagcgcg gcggttggtg
     2881 ggcattatgg aacacggcgc ataacgtcgg cggcgcactc attcccattg tgatggcagc
     2941 gagcgcgctg cattnnnnnn nnnngcgtgc cgggatgatg attgcgggtt gtatggcgat
     3001 agtcgtgggg atttttctct gctggcggct gcgcgatcgc ccgcaggcgt taggtttacc
     3061 ggcggttggt gaatggcggc acgacgcact ggagattgct caacaacaag aaggggcagg
     3121 gctgacgcgt aaagagatcc tcaccaaata tgtgttgctg aatccgtata tctggctgct
     3181 ttcgttttgc tatgtgctgg tctatgtggt tcgggcggcg atcaacgact ggggcaattt
     3241 gtatatgtcc gagatgctgg gcgtcgatct ggtcacggcg aatacggcag tgacaatgtt
     3301 tgaactgggc ggatttatcg gtgcgctggt agcgggttgg ggttcggaca aactgtttaa
     3361 cggcaaccgc gggccgatga atttgatttt cgccgccgga attttgcttt cagtcggctc
     3421 gctgtggctg atgccatttg ccagctacgt gatgcaggca acctgcttct tcaccattgg
     3481 tttttttgtc tttggcccgc agatgttaat cggcatggcg gcggcagagt gttcccacaa
     3541 agaggcggca ggggcggcga cgggatttgt cggcttgttt gcttatcttg gtgcgtcgct
     3601 tgctggttgg ccgctggcga aagtactcga tacctggcac tggagcggat tttttgtggt
     3661 tatcgccatc gccgccggga tttccgcact gctattactg ccctttttga acgcccagac
     3721 accgcgagaa gcgtgatgca tctcaccttt tcacttcata tccggcaaaa ctaagaaatt
     3781 ttccaggttt tgcctggacg ctatctcagg cctgatttgc tgctgatttt tacaatgcat
     3841 gcctcacgca ggtattcatt tcaggagtaa cccatgctgg ctttcttaaa ccaggttcgc
     3901 aagccgaccc tggaccttcc gctcgaagtg cggcgcaaaa tgtggttcaa accgttcatg
     3961 caatcctacc tggtggtctt tatcggctac cttacgatgt acctgattcg caagaacttt
     4021 aacatcgcgc agaacgacat gatctcgacc tacgggttga gcatgacgca gctggggatg
     4081 atcggcctgg gcttctccat cacttatggc gtgggtaaaa cgctggtttc ttactacgcc
     4141 gacggcaaaa acaccaaaca attcctgccg ttcatgctga tcctgtctgc catttgtatg
     4201 ctgggcttca gtgccagtat gggcagcggc tcggttagcc tgttcctgat gattgccttc
     4261 tacgccttga gcggcttttt ccagagtacc ggcggttcgt gcagttactc caccatcacc
     4321 aaatggaccc cgcgtcgtac acgcgggaca t
//
LOCUS       DAWDRC010000340         4317 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8568_length_4317_cov_2.454951, whole genome shotgun sequence.
ACCESSION   DAWDRC010000340 DAWDRC010000000
VERSION     DAWDRC010000340.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8568_length_4317_cov_2.454951"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1242 a          971 c          824 g         1280 t
ORIGIN      
        1 ccagagttgc atagctaaca ggattatcca gttctgtttc agggagcttg taattagcct
       61 gacatttttg ctgaacaccg tctccccact ggatattaat aacaccttct aaaggcaatc
      121 ctgagagata aagttgtcca ttatcgccga caatcccggt aatttgacta ctgtttttaa
      181 tctgagcgct ggctgtagca ccgaatggga ctgatttatt acgataacga actgtgaaca
      241 aaccacgaat accgatatga gcagcaaact ctgcacgaac taacgcacct tttcccggaa
      301 taacttcagt ggaagtgata tccagttcag tatgcatatt cattgacata ggatctaatg
      361 aaatattatt acgtctatat tctgttgcat aaggaacaac tgcatatcca cgccagtcag
      421 tggatattcc tgtcccccgc tgaacatcca cattattcgc tccaggcgct tttatcaaaa
      481 cattagtttc acctaatggt tgactcaacg taagaccatt ttcatgtaca acaagagcac
      541 cactgatgcc atagtttatt tgttgattac cttcattatc gtaactgtag gcggaattca
      601 aggaaccata tgccccacga taattaacac caacagaacc actactgcta ttatcctgat
      661 tagcaaaacc atgttgtatg ttataactga gattatgctg ctccaacaga gaaccagata
      721 cccccgtata ttggtttacc tgtccgtggt tatcctgact ataaccatac gttgcagtca
      781 tggcattcga tgatgagcgc ccatcattcg tggaagataa ccaattactt ataggtaatg
      841 agacatttag cgagacaatt ttatccgcat ttgtccctgg tgatttactg taattccacg
      901 caacatttag atagataccc ttaatactgg tgttataacc aacttggtat aaagaatctt
      961 ttttatcagt attccagtat gtttgctgac tagccgataa atacatagac ccgtagttac
     1021 cgaaaggttg cgatacacta gcctgaaatt ttccacgttt gctataacgc aagttataaa
     1081 aatcattcca gttataaacg taatctttat catctaacgt ttttggatca acaacagtcc
     1141 ctgacatttg ttggtatgtc gtatcactta aagtgtaaaa gccctgggtt gaataacgat
     1201 agccaataat atggaatgct gttcctgtct gaactaacga tttggaataa agaaaacgat
     1261 aagattgccc ttgatgtttg ctcccatcca caagcgaact gttagcctga gtaacatcaa
     1321 acgatgttgc accaaataca cccagattca ggccaaggcc gagcgccaac gcccggtaat
     1381 cctcagagaa ttgtgcccca ccataagccg taatatccca gggtaaaccc caaattaatt
     1441 ccccctgaaa tacataagga cttttctggt tatagctatt gcttcgatat tggccaaccg
     1501 tagtagaata ttttaaatga ccttctcgtt gcaggatggg gacagcggca taaggtacgg
     1561 tatagacagt ttctgaatta tcagactctt tgatggtaac ttccagatct cccgcagatg
     1621 atgtcgggtt aagatcgcta atctcaaacg gtcccggcgg catataggtt tgataaatga
     1681 cataaccatt ctgtttgatt gttacctgag cgcgactttt cgcaattccg cgcaccgtgg
     1741 gagcgaaacc actttgacta tcgggcaaca tattttcgtc agattccagt tttatgccac
     1801 gaatactaac actgtcgaaa acatctgatg atgaccatgt atctcctagc gttaattcac
     1861 ttctgaaagg aataataacg cgctgcaacg tactactgat atgctccagt ttacctgctg
     1921 aattactccc acgactccag gttgaataat cgcgaaaacg ccatgcacca atattgatcc
     1981 cagcgcgaag atttaaataa tagttatttg aagagtaatc accacgatcg ttattaccag
     2041 aaaagtcata attcagcaaa aaagctgtaa taccgttatc ccactgttct ggtggaatat
     2101 atccgcgagc gtcacgtttt attatcgcct gtggaaaggt cagtttaagt ctctgtgcat
     2161 ccagctccgg catgtaatgc acatcgggaa accagatacg caaatcaagg caactatttt
     2221 cagatgaagc caaagcctgc tctggcagcg catttttgtt aattccaagg cttaataagt
     2281 cagcaagtga aaggcaggga atcagttgat tctcatcgtc tgctttaaaa gttacagtac
     2341 gagagagtaa aggggaatca ttgacccaaa cttcgacttc atagctacca ggaagataag
     2401 tcatcccttt ctcgaagcgg gataagtcag caacagcttc agtatcaccc gacagaaaag
     2461 cagggttaaa gcgcattccc gcgattgaac atgcaggcat aataaccaga agaagcggca
     2521 caacccgttt catctgcttt tgtgccgcca tccaatattc gcatcttttg agctttaggt
     2581 tcatatccct gacctgtgtt gcatctgaac tattccgttg ttactgcatt attcctttct
     2641 gccgctccgt cgtagcgcca aagtcattaa ttgtttgata agtaatttca ccagccaacg
     2701 tcactggcga cgagaccgta atatcacttt tcggtggtac aaacatattg ggcaatttac
     2761 tcccttgtac tttaattcct gtcagtgtca ggtaataagg cgaaggattg ataactttta
     2821 gttgtttgcc ctcatactta aaccgcaaca tatttcgtgc ttttcctggt tgcacaggta
     2881 gattttccgg gcgataaaat agcttgattt tgttttgtaa tgccaactgt aaggtattct
     2941 tctgtgtatt tttctcatct gttggcggaa ttgctttaac attcatccaa tacaaagttt
     3001 cacgatcggt cggcaaatta gtcttaccaa gaaacatgat acgtaatgaa gcttctttac
     3061 gcgttggtaa cataaagagg ggaggagtga taacaaaatc ctggctcttt ttttccgttt
     3121 tactgtcatc aacccatgat tgaattaaat aactggtgtt gtcaccgcta ttttgcacac
     3181 tgagtgttat ttgcttactt tctgccggat aaatcaatcg cgttacgcca aggccaaacc
     3241 cgcccgcgga cgcattatta ataaataata tcgatagtaa aaaaagaatc cctgagcgta
     3301 taatattttg tgatactttt aattcatccc tgaatccata aacccatttt ttatttccaa
     3361 taatacattt catacagcat ccttgttgat gaaataacac ctgttattca tactctatta
     3421 aataatttca atctcaggtt acaacttttt gtaacctgaa atcgtattta tatttactcg
     3481 tacagcacca caaaacttgc tgttgactta actacaccag gtgcaacagt gctaccgttt
     3541 tgagcataac ctgcagtaaa ggccgcgctg aaatcatggg tcgtagcatt tcctggaatt
     3601 gtataagcgg cggtggaggc gctaccctct gccgcagtag tattcaggtt aatcagacca
     3661 ttattcgtcg tacctttgga gtaaattcga atccctacac cagtcgcccc cgcattgccg
     3721 ctgttgtcag catccgttga tccttctact tcgttagcga gcgttttatt agcgctatct
     3781 gcagtaccgg taaaacggaa ctcaattttc tgatcctctg tattgctaca ttcggtcaga
     3841 ttaatattga aatcggtctg gttgctcgcc ccctgtgctt ctacgtcgat accagtgaaa
     3901 aggttggaag gcacaaccgg caggacaact tcaatctgtt ttgaatcggt tgcgacctta
     3961 caggtagctt tcactacacg gccagtaaag cggattttcc cccccattga atcaacgttg
     4021 tcatccatat cagtgagcct gggataatca acggtgctcc agtcagtatc cgccctacca
     4081 aattgtgctg catcgccggg tgctgcggtt accgcagtag acatcagcat acctgatgcc
     4141 agtaatactg cggataaatt agataaattg aatttcattt atacctttcc ttttgttaaa
     4201 aaatccaagc aacattggtg tttttacatt ctgcgttaat aagttatatc tcacaaaaaa
     4261 aataagctga cgttggctaa ttccagaagg ttctttagaa gagtaattga gtgaata
//
LOCUS       DAWDRC010000341         4315 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8575_length_4315_cov_2.119484, whole genome shotgun sequence.
ACCESSION   DAWDRC010000341 DAWDRC010000000
VERSION     DAWDRC010000341.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8575_length_4315_cov_2.119484"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          953 a         1132 c         1177 g         1053 t
ORIGIN      
        1 aagaaggcgg aataacccgc tacgcgtacc accagatcgc ggtattgatc cggatgtttc
       61 ttcgcctcca gcaatgtttc gcgggaaaca atgttgtact gaatatgcca gcctttatgc
      121 acttcaaaga aggtacgcag caggatcatc agtttctgct tgtcagattc attctccagc
      181 gttgccggat tcagtttctg gttgagcagc acgccgccga gaattgctgc cgtaggcagt
      241 ttacccaccg agccaatgac cgcagtaggg ccgagatggt cagtaccgga ggccggacta
      301 gcgccttctg ccagcggggt atgtgcttta cgtccgtccg gcgttgccat agtctgcgcg
      361 ccaaacggta cgttagcgga gatagacgat gtgcccgcat aatagttgcc gccaatcgga
      421 ccacgaccgt agcgcgggtt atggtactgc ttcagttcgt cgatataggt ctgataagcg
      481 cgagccagca gcgtatcgac agtatcatcg tcgttgccgt acttcggcgc accgttaatc
      541 agccgctgac gcagctgctc gtgagtcagg ccgtcgaagt catccgccag tgcggcagcc
      601 agctgttgct gaccaatcgc gccctgttca aataccagtt tcttcactgc cgccaggctg
      661 ttgccgaggt tagcaatgcc gacctgcagg ccagaaaccc agtcatattt cgcgccgcct
      721 tgcttgatac ttttcgctcg ctcaatacag tcatcgacca gcgccgagca gagaatatcg
      781 tgtacgttct cttccagcat ggtatcgacg acatattcga tttcgattga tttacgcgtg
      841 tagtaacgga tttgcgtatc ccatgcgtcc attacttcat cgaagttgtt gaagttaccc
      901 gctgacaacg ctttttcttg tggcaggaac actttgccgc tggtggcatc acgaccgcct
      961 tccagcgccg ccagcatcac gcgggcgaag ttgataaagc tcatgccggt acagcgatag
     1021 ccccatttgc caccgacggc ggtttcgata caaccaatcg ctgcatagtc gtaagcgtcc
     1081 tgcggttcaa taccgagttt aataaattcc gggatgacga tttcgtcgtt gttgaacgcc
     1141 ggcatcccga agccgcagcg gatcacctga acacaggcgt cgaggaaatc gttactcata
     1201 cccgcatggt aacgcacgct gaggttaggc tgagtcgaac gcaggcgacc gcaagattcg
     1261 aggatcgcat aagagagtgg atttaccgcg tccatcggtt gaccatcaac cagattttgc
     1321 ccgccaatgg tgacgttctg atacagcgga cttcccgcag aggcttttga gtgtgagccg
     1381 gagcggattt tgttcacttc cagcaatttc aaccagcagc tatgcagcat ctcgatggcg
     1441 tgttcgcgat ccagtgtctg gttcagttca acgtcacggc gatagtatgg atagagatac
     1501 tggtccatac gaccaaacga taccgagtga ccgttagatt cgatctgcaa aatcaactgg
     1561 atgaagtaac atagttgcag cgcctgccag aaagtctgcg gcggctggtg ggcgataaga
     1621 tcgcagtttt ctgctatcgt cagcagttca tcgcgacggc tttcgcgggt ttcggtcgcg
     1681 gccatttcac gcgccagggc agcgaaacgt tcaatgtgtt cactgactgc caccagcacg
     1741 atatcaatcg ctttcaggaa ttgctcgccg tgcagatctt ccagaaccgt cagattgatg
     1801 cgcgagcgac gttccgccac tttctcacgc agaccatcaa gacctttttc cagcagtagc
     1861 gggaaattga ccgccaggtg cgcatcgccg gaagtcatat tgccctccgc tttaatgatt
     1921 ccggtcgcca gcagaccttt ttgctcatcg gtaaacatgc cgtagcagcg atcctgtacg
     1981 gtctgaccgc gccaccacgg gcacacttca tgcagaacgc gtttgttctc ttcgctcacc
     2041 gcaaaaccag caccgggacg atctgccaga tcatcaatct ctttttcgat ccacgagacg
     2101 gtatattccg ggaagatcgg cgcggcgcga acttcgcttg cctggttgcc aatgatcaat
     2161 tcatcatgtt tgatccagat ggtgcgattc gccaggtgat gcgccagtgc cagcgcgcga
     2221 cgtaccggaa tgggcttgtc gagatgttgt tgatacatct cggtatagtg ctgcgcgcgc
     2281 tcggtacaga ctggtggttt cacaatatgc accagcgcat ttttgtgcgc tttaatgcgg
     2341 tcgctgagcg tgtccagttt cagtgtggtc atggttgtta tcctcgtaag gtcgcggtta
     2401 accctttctg gcaggcatac tgctgggcaa agtcgagcag ttctggcgca tcaagcggtt
     2461 tttccggggc gtcatagggc agattaagta agtgatattt gttgatgcct agcgtgtggt
     2521 agggcaggaa atggatttcg ccaacgtgca gctcgtcggc ggcaaaatcg gtaatggctt
     2581 tcacagaggt ttcgtcggca ttaaagccct gaatcagtgg cacgcggata atgatttttt
     2641 tgcccgctgc ggcgagtttt ttcaggttat ccagcaccct ggcggcgtta ccgtcggtcc
     2701 actgtttaaa cggcgcgtcg gcaacgtgtt ttaaatcggc aagaaacaga tcgatatagg
     2761 gcagagaagg ggagatatat ttccacggca catgcagaca ggtttctacc gcagtatgaa
     2821 tgccggcctc gtggctggct tgcagcagcg ccgtcgccat ttccggctgc ataaacggtt
     2881 caccgccaga aagcgttaag ccgccgccgc tgcgatcgta aaacggttta tcgcgcaaaa
     2941 cggttgccat gatctcctca acgcttttca cttcaccaca cacggttaac gcctgtgtcg
     3001 ggcagcagtc ggttagcgcc gtaaaatgct ccggggttaa cttttcccga tgaataagca
     3061 aaccattcaa cgcgcgctca atcacttccg gtgcggtctt agcgcacagc tcacagcctt
     3121 ccagacagag tcgtgggtca tacagcagat cctgcgtgcg ggcgcggctt tccgggttct
     3181 gacaccagcg gcagcccagc gaacagcctt taagaaatac gaccgtgcgg ataccggggc
     3241 catcatgggt cgagtagcgt tgaatattga aaatcatagt tgcctctctg tttcgttcaa
     3301 atattaaaat actttcgaat gaaagttaga ttgatgtgcg tcaactgttc agagagtttt
     3361 cccgtgatag tctacattca gacaaaaagt acattttgag gatggttatg gaactgtatc
     3421 tggatacttc agacgttgtt gcggtgaagg cgctgtcacg tatttttccg ctggcgggtg
     3481 taaccactaa cccaagcatt atcgccgcgg gtaaaaaacc gctggaggtt gtgcttccgc
     3541 aacttcacga ggcgatgggc ggtcaggggc gtctgtttgc ccaggtaatg gctaccactg
     3601 ctgaagggat ggtgaatgac gcacgtaaat tgcgttctat tattgcggat atcgtggtga
     3661 aagttccggt gaccgctgag gggctggcag ctattaagat gttaaaatcg gaagggattc
     3721 cgacgctggg aaccgcggta tatggcgcag cacaagggct actgtcggcg ctggcaggtg
     3781 cggaatatgt tgcgccttac gttaatcgta ttgatgctca gggcggtagc ggtattcaga
     3841 ctgtgaccga cttacaccag ttattgaaaa tgcatgcgcc gcaggcgaaa gtgttagccg
     3901 cgagtttcaa aaccccgcgt caggcgctgg actgcttact ggcaggatgt gaatcaatta
     3961 ctctgccgct ggatgtggca caacagatga ttagctatcc ggcggttgat gccgctgtgg
     4021 cgaagtttga acaggactgg cagggagcgt ttggcagaac gtccatttaa ccggacgttc
     4081 tgcatcctca taaattgctg atgacgtggc ggagtgccgc gtcttatctg gcctggaggt
     4141 ggcagttact gcccgcacac ctcacacccc ggattgcgca tcagtttcat ttcgcgaaac
     4201 tgacaggtca tcgcatcgta catcacgatt tttccgctgg ccggtttgcc ataacctgcc
     4261 agcagtttga tcgcttccat tgcctgcaac gaaccaatca ccccgatcaa cggtg
//
LOCUS       DAWDRC010000342         4314 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8577_length_4314_cov_2.054238, whole genome shotgun sequence.
ACCESSION   DAWDRC010000342 DAWDRC010000000
VERSION     DAWDRC010000342.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8577_length_4314_cov_2.054238"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3820..3829
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1196 a         1034 c          935 g         1139 t
ORIGIN      
        1 gcctttgctg catcaatcgg taaaggtgcc accctccata aaccgaaatt ttctgaagca
       61 ttgcgaaccg gccttatttt tggtgccgtc gaaaccctga cgccgctgat cggctgggga
      121 atgggcatgt tagccagccg gtttgtcctt gaatggaacc actggattgc gtttgtgctg
      181 ctgatatttc tcggcgggcg aatgattatt gagggttttc gtggcgcaga tgatgaagat
      241 gaagaaccgc gccgtcgaca cggtttctgg ttactggtaa ccactgcgat tgccaccagc
      301 ctggatgcca tggctgtggg tgttggtctt gctttcctgc aggttaacat tatcgcgacc
      361 gcattggcca ttggttgtgc aaccttgatt atgtcgacat tagggatgat ggttggtcgc
      421 tttatcggct caattattgg gaaaaaagcg gaaattctcg gcgggctggt actgatcggt
      481 attggcgtcc agatcctctg gacgcacttc cacggttaat aagaacgctg ccagaggtga
      541 atattaaagt ccgtctggca gtcgaacact tcttttgcgg ccagtgcttg ccagacttca
      601 ggcttcgcac gccaggcaaa cggcgtcatc tgcaataatg cgacggcttc atcaccgcga
      661 agacgcatca gataacacaa ctcatcactc tgctgtaatg taaaaccttt cagttgttct
      721 gcatgaggtg catgaagatg tacttcattg taaatcagcc ccttcagctc cattaagtgt
      781 ctcggtcccg gcgtggcagt aatgacccaa ccgccgggct tcactactcg cactaattct
      841 tctgctttac acggcgcgta aatacgtatt atggcgtcca tactggtatc ggaaaacggc
      901 aaacggtggc tggaagcgac acaaaaagtg acctgcggat agcgtttcgc cgccgctttt
      961 atcgctacct tcgagacatc caggccaaac gtggtgattt cgggcaacgc atcagcaaat
     1021 gcgtgtgtgt aatacccttc accacagcca atatccagca ccgccgcggc cttttcatca
     1081 agccgttccc gcagttggcc gacaattgca tcacgcagcg gctgataatg tccggcatct
     1141 aagaatgcgc ggcgtgcttg catcatttcc gcgctgtcgc ccggatcacg agaccgttta
     1201 tgctgaacgg gcagcagatt gacataccct tctttcgcca tatcaaactg atgtcgctgg
     1261 ggacagatat agctgttttt ttcacgcgaa agaggctgat ggcaaagtgg acaggaaaac
     1321 gacatgacaa ctcgggcagg atactaaagg ccgcaagtgt aacgcgaatc atgcggcggg
     1381 caaatatccg aacgactgga gcgaagataa aacaaagtaa aaagcctcgc attcgacgag
     1441 gctttatagc gagattgaag cgtattcaca cttcagatca gtggattcga tcagatagct
     1501 gttacgttaa cagctgccgg acctttctga ccgtcctgaa tttcgaactc aacgttctga
     1561 ccttcagcca gagttttgaa accattaccc tggatagcgg agaagtgtac gaacacatct
     1621 ttgctgccat cagccggagt aatgaagcca aaacctttag actcgttgaa ccacttaacc
     1681 tgacctttaa tctttgccat ttgaaaaatt ccttagattg ttttcttcgc ccgcaggcat
     1741 aacatagata aaactgacac attactgcat gaggcaccaa tataaggctc ggcagagaag
     1801 cggtattcaa cgtcaacgtg tttactcagg acttctttac tgaaaatgcc acacataaac
     1861 agaactgtac ctcgtttaac ccgaaatctg ttatcacata caacgttaat tatggcaagc
     1921 catttttaaa catgtctcga tcagacgcac aaatctcgat acgtcttcca ctttttttgc
     1981 acacttatgc aacgaaatac gcgccgatat atcattgtgc ttaaccttgc cagttcaggc
     2041 agatacttaa actggcgtat tttctaacat agttcaataa gttcagttgt tccaggccag
     2101 tgggagaagt tattacatag tgcgtgcaat atcacatttt ttgctatgca atgaataaaa
     2161 agttatatca ctttttctca taaaacagtc agttaacggc tatcaattac cctaaagaga
     2221 agtcagtccc caaagggatt gtaaatttaa aataagaaaa attgatgaat gagcaaaaaa
     2281 atcaagagag aaacatttcg ctaataaata ataccacact taatcacaaa tccgatgtaa
     2341 acatcctaat aaattaatgg ggtaaatatc tttttctgat atcccgttac gcaaattaat
     2401 aatatgataa ttatttgaag tcagaccaga gttttaattt gaagcagctg caccatgaca
     2461 gtgcgtataa tcagcatgaa ttgagtaacc cttcaacgac ggggttactc aagattgagg
     2521 aaggattatc gttcggcgac cagacgaata atatcttcgt cttcaaccga tttttgactc
     2581 tcaacggtct ttttatctgc tttctcaggt tcgtttgctt cgcacaaacg acgtaacagt
     2641 gcattttgac gtttttgctg atccagcaac gcctcaagca gttcaatctg ttcgttagtc
     2701 cgtgaactgg cacgattgat aaaaaaccac aagatgagcc cgataagaag aaccaccacc
     2761 gatacaacca aagacgcaat gttcatcacg cctgaattta caacttcgtt catttcacca
     2821 cctctgagta gaggcgctat tctaccactg ctggagagga agaaaatcta gtgctgaaaa
     2881 aatgatatca ccacgggata aactggttaa tggcacaaat tccgctgaaa aattgtacat
     2941 cctgatcgca catcatgttg aatacctgcg cccaaagcag caagcaagcc aacaccacga
     3001 caaccagagc gacccatcga aactttttca ctccaatctc cgtttcacct accttatgtc
     3061 attaaactag catgcgttag ttaaacagcg cctaactatg tcgatattcg tgctttttcg
     3121 gaatgagtcg cttgcttcag cgcatattgc cgctacgatt aagcgaacat aaaaagagaa
     3181 gaggttgtaa tgcgattcat cattcgcaca gttatgctga tagcgctggt atggatagga
     3241 ttattactca gcggatatgg cgttttgatt ggaagcaaag agaatgccgc gggattaggt
     3301 ttacaatgta cctatctgac cgccagaggc accagcacgg tacaatacct gcacactaaa
     3361 agtggttttc tggggataac agattgcccg ttgttacgta aaagcaatat tgtggtcgat
     3421 aatggttaag gataataaat aaccagattc tcactcatga gtaaaatatt cttatcgcaa
     3481 gtgcgtttaa tataacgcca ccttcaactt tatttttctc atgctaaata aagaacctgt
     3541 ctgtttgata ttcagaaagg ttcttactct catcagaacg gatagtcgtg ataacccatt
     3601 tgggcagaaa ttttgcgcgc cgcggtatgc aacattgcga catattcctg taaacgctct
     3661 tcagagaaac gcaacgtcgg gaaggagatg ctcagaccgg caatgaccac gccaaagcga
     3721 tcaaataccg gaaccgcaat gcaacgcagt ccttcttcct gctcttcatt atcttcgcca
     3781 tacccctgct cgcgcacctg gtccagaacg ggtaataacn nnnnnnnnng gtaataacgc
     3841 ttctgtactg gtgatggtcc gctcggtact gcgtttatac tccacgccct caagaatttg
     3901 catcacttca tcgcgatcgc gccatgccag cagtacctta ccaatcgcgg tgctgtacag
     3961 cggattacga cgcccaatcc gtgaatacat gcgcaaattg tacattgagt caattttgtg
     4021 gatgtaaaca atactatctt cgtccagtgc gccgaggtgg atagtttctt tggtcaggcg
     4081 ggagatctca cgcatctgga tatctgcgct acgaattaaa tcgacgtttt gcaacgcgcg
     4141 agcgcccagt tcaaacaatt tcagggtcag cgaatatttc tccgactccc cttcctgcgc
     4201 cacataacct aaggttttca tggtctgtaa aaagcgataa acggtgcttt ttgacatcat
     4261 aacgcgctgc gacaactcgg ttatccctat ttcgcgctct tcacccagcg cctg
//
LOCUS       DAWDRC010000343         4314 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8578_length_4314_cov_1.969242, whole genome shotgun sequence.
ACCESSION   DAWDRC010000343 DAWDRC010000000
VERSION     DAWDRC010000343.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8578_length_4314_cov_1.969242"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3993..4002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1037 a         1000 c         1174 g         1093 t
ORIGIN      
        1 agcgtgttct gcctgctgaa gagtatgcgg aaattcgctg ggatcaactg gaagcgggtg
       61 acgttaactt tattaaagat ttttactacc gtctggcgca tcgcgtgggt gagttgagtc
      121 acctggctga tggttcatat gccatcgcag aacgctggaa tttgggtgaa gagtactggg
      181 gctacgcgaa aaataaactc tggtcgccgt ttggctatcc ggttcaccat gccaatgaag
      241 cgtcagcgca agtcggttcc attgttaact gtatgttcaa ccgtgactgc atgacgcata
      301 cccatatcaa ttttattggt tccggcttgc cattgaaact gcagcgtgaa gtggcgaaag
      361 agctttttgg ttctgaagat gcttacgatg aaaccaaaaa ctacacaccg atcaatgacg
      421 caaaaattaa atatgctaag tggtcgctgt tgcgggtctg tttgcataac gccgtcaccc
      481 tgtgcaactg ggtctggcca atgaccgttt cgccgctgaa aagccgtaat taccggggcg
      541 atctggcact tgaagccaaa ttcttcaaag cgatcactgg cgaagatatg actcaggaaa
      601 aattagattt agctgcagag cgtatcttta cgttgcatcg cgcctacacg gtaaaactga
      661 tgcaaaccaa agatatgcgt aatgaacacg atcttatctg ttcctgggta ttcgacaagg
      721 atccgcagat cccggtcttt actgaaggta cagacaaaat ggatcgtgac gatatgcatg
      781 cttcgctcac gatgttctac aaagaaatgg gctgggaccc acagcttggt tgtccgaccc
      841 gcgaaacatt gcagcgtctg gggctggaag atattgccgc cgatctggca gcacacaatc
      901 tactgcctgc gtaaggaatg ggagagatga accatcagga tgaattgccg ttagcggaag
      961 tcagcgaaat tgatgaggcg aagcggcaat ggttgcaggg gatgcgtcac cccgttgata
     1021 cggtaaccga accagaaccg gctgaaatcc tggcagagtt cattcgccag cattctgcgg
     1081 ctgggcagct ggtggcgcgc gctgtgtttc tctcgccgcc ctattcggtt gcagaagagg
     1141 aactgtcagt tttgctggaa aacattaagc agaacggaga ctacgcggat atcgcttgta
     1201 tgacgggaag ccaggatgat tactattact caacgcaggc catgagtgaa aactacgcgg
     1261 cgatgtcttt gcaagttgtg gagcaggaca tttgtcgcgc cattgcacac gccgttcgtt
     1321 ttgagtgcca gacgtatcct cgtccttaca aagtcgccat gctgatgcaa gcgccatatt
     1381 acttccagga agcgcagatt gaagccgcga tcgccacgat ggatgttgca cctgaatatg
     1441 ccgatatacg gcaggtggag tcatcgacgg cagtgttgta cttgtttagc gagcgattta
     1501 tgacctatgg caaggcgtat ggcttatgcg aatggttcga ggtagagcag tttcagaacc
     1561 cctgaaattt cagggggatg tcatggatag ggtggacagt tgcagccact gcattagggg
     1621 ataatcagat gtccttcact cgacgcaaat ttgttctggg gatgggaaca gtaatctttt
     1681 ttactggttc tgcgtcttct ctgttagcga atacgaggca agaaaaggaa gttcggtatg
     1741 ccatgattca tgacgagtca cggtgcaatg gctgtaatat ctgcgcccgc gcatgccgta
     1801 aaactaacca cgtccccgct cagggaagcc gtttatcgat agcacacatt ccagtcatcg
     1861 ataacgacaa tggaacgcag tatcactttt ttcgtcagtc atgtcagcac tgcgaagatg
     1921 ctccttgcat cgacatttgc ccgacagggg cgtcgtggcg tgatgagcag gggatcgtgc
     1981 gggtagaaaa atcgcagtgc atcggctgca gttattgcat tggcgcttgc ccgtaccagg
     2041 ttcgctacct taaccctgtc actaaagtgg cagataaatg tgatttctgc gctgagaccc
     2101 gactggcaaa aggctttccg cccatttgcg tcagtgcctg cccggaacac gcattgatct
     2161 ttggtcacga agatagccca gaaattcagg cgtggttgca ggaaaataaa tactatcaat
     2221 atcagctacc tggcgccggg aaaccgcatc tgtatcgccg gttcggtcaa catttgatta
     2281 aaaaggaaaa tgtatgaacc cgtcgcaaca tgctgaacag tttcagagcc agttggcgaa
     2341 ctatgtgcca cagttcaccc cccaattttg gccggtgtgg ttgatcattg ccggactatt
     2401 gctggttggg atgtggttgg tgctggggct acatgccttg cttcgtgctc gtggcgtgaa
     2461 gaaatcagcc accgatcatg gtgagaggat ttatctttac agcaaagcgg tcagattatg
     2521 gcactggtcg aatgcgttac tctttgtatt gttgctggca agtgggctga taaatcactt
     2581 tgcgctggtg ggcgcaactg cggttaaaag tctggttgcg gtgcatgaag tttgcggatt
     2641 tttgttactg gcatgctggc tcggctttgt tctgatcaat gccgttgggg gtaatggtca
     2701 ccactatcgc attcgtcgtc aggggtggct ggagcgagcg gcaaaacaaa cgcgattcta
     2761 tttgtttggc attatgcagg gggaagaaca tcctttcccg gcaacaaccc agtctaaatt
     2821 taatccctta cagcaggttg cctatgttgg tgtcatgtat ggattgctgc cgttgttact
     2881 attgacgggg ctgctgtgtc tctatccgca agccgtggga gatgtgtttc ctggcgtaag
     2941 atactggtta ttgcaggcgc attttgctct ggcatttata agcctctttt ttatcttcgg
     3001 tcatctttat ctttgcacca cggggcgtac gccacacgaa acctttaaaa gcatggtcga
     3061 tggctatcac cggcactaac gaatgatcat tacacgagca gacctgcgag aatggcgtat
     3121 cggagcggtg atgtatcgct ggtttctgcg ccatttccca cgtggcggta gttatgccga
     3181 tattcatcat gcactgattg aggaaggata tacggactgg gcggagagtc tggttgagta
     3241 tgcgtggaaa aaatggctgg cggatgaaaa cttcgcgcat caggaagttt cttctatgca
     3301 aaaactggcc acagatcccg gagaacgact attttgcagt cagtttgccc gcagcgacga
     3361 tcatgctcgc atcggctgtt gtgaggataa cgccagaatc gcaaccgctg ggtatgccgc
     3421 gcaaattgcc agtatgggtt atagcgtgcg gattggaagt gtcggcttta acagtcacat
     3481 tggcagctcc ggtgcacgcg cacgggttgc tgtaaccggt aattcttcgc ggataagcag
     3541 cgctggagat agcagtcgga ttgcgaatac cggaatgcga gtacgcgttt gtacattggg
     3601 tgaacggtgt catgtcgcca gtaatggtga cctggtgcaa atcgccagct ttggcgcaaa
     3661 cgccagaatt gccaactgcg gcgacaatgt acacatcatt gccagcggtg aaaattcaac
     3721 agtagtgagc acgggcgtgg tcgactcaat tattctcggg ccagggggat gcgccgcact
     3781 cgcatatcat gacggcgaaa gagttcgctt cgccgtcgcc attgaaggtg aaaacaatat
     3841 tcgagccgga gtccgctatc gtcttaatga acaacatcag tttgttgagt gctgatagct
     3901 ttacctttaa tcaaacgaaa gacgccgacg cgcacgggat gcaggtttta cgaccgcccg
     3961 agccatcgcc tctccaattt ctgcacatgc cgnnnnnnnn nntccctgaa cgaaaggctg
     4021 gtcgtgcatc aaccagggca acgcgccgaa cgctacgcgc ccacgaatat cttcgggttg
     4081 ctgtaacgta taatcttcgc caacatcagg gatttcatcc cctgttttct gtaattgttc
     4141 gcgtagtcca gggaaaggaa tatctttcac tttaagcgga cgctgcccgc gggcatcaat
     4201 aaaaacgtca aacgagtaac tgttgtcttc cgttttcagg acggtgcgcg actcattaat
     4261 ttccatttcg tagtcttcac cgagggcgag aatatgaatg attccagctt cacg
//
LOCUS       DAWDRC010000344         4306 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8596_length_4306_cov_2.112444, whole genome shotgun sequence.
ACCESSION   DAWDRC010000344 DAWDRC010000000
VERSION     DAWDRC010000344.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8596_length_4306_cov_2.112444"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    753..762
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1353..1362
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3008..3017
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1069 a         1125 c         1057 g         1025 t
ORIGIN      
        1 gttggacgga accggcgaag gggtcgccca ttgcataatg ctgggatcga agttcagcag
       61 ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg ccagtttgcc
      121 accgatagag ttcgccacaa aagcagcaaa catcggaatg gcaaaaccaa acaacacacc
      181 gccgaaggac tgcgacagcc aggcaaattt aagtaaggag aggtcgaaac cggagaattt
      241 cccgctatta agagcatcca tgatgccgat atcagcagga attttaagcc agctataagc
      301 aatcagctga ctgaaagcga ggatcacacc gcccataatt aaggtcggca ccatgcgtga
      361 aatccctgac atcacatgct gcgggagttc accccagaag ctggatttag aggcctgcgg
      421 attcttaact gccgctgccg caccggatgc gccatgcaca acggttgcac tgcgtttttt
      481 aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc
      541 gatttctttg atgattcccg cagcgttttt aattgcgtcc tgcaaagtga tttcataaac
      601 gtcgcgtgat tcgaaacgtt cgttatcttc cggggtaact gccacggagt ggatgatgat
      661 ggtcgcttcg gcgatatcct gcgccgtcag gcgattctgg ataccgtccg cgccctgggt
      721 ttcaattttc acttcataac cggcttctac cgnnnnnnnn nnccgccgct tcttccagcg
      781 ccggagcggc cataaaagtg tgagccaggc ccattgggca ggcacataag gcaatcagtt
      841 tcttactcat cgttaattcc tcgttcagtg atagttgacg agtgaaatta tgaacgtgca
      901 aaacaaatcg ccgttaccag acaaataatg catttactgg agaattaatc ccgctgtacg
      961 gaagtgtgac gcaggtcggc cttgtgaggg ggacacaaag aaaatttaat taactgtatg
     1021 atttaaaaga ttatcgggag agttacctcc cgatataaca ggaaggattt agagaatgtg
     1081 acctaaggtc tggcgtaaat gtgcaccgga accgagaaga cccggattgt catggacgat
     1141 gagatacacc ggaatatcat ggacatattc tttaaagcgc cctttatctt caaatgcggc
     1201 gcggaaacca gaggctttga agaactcaag gaagcgcggc acgataccac ccgcaataaa
     1261 cacgccgcca aatgtcccga gattgagcgc cagattgccg ccaaaacggc ccataatgac
     1321 gcaaaacagc gacaacgcgc ggcggcaatc ggnnnnnnnn nnatacaaat tcaccagtcc
     1381 agggccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca atatttcgag
     1441 gataatgccc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt ctcctggcaa
     1501 gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccctg ttccggcacc
     1561 gtaaaccgca ataggcttac cttctaccgg ttctgcgcca ccaaactgaa tcagatgctc
     1621 ttttttcagc atcgggatcg ccatcgatac agcagtaaaa tcgttaataa tttccagatg
     1681 gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat ggttggtcat
     1741 tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt tcacctcgac
     1801 cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat caagccctga
     1861 ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa gacgtgcgtt
     1921 ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc tccgctaaag
     1981 tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac gccggaaagg
     2041 caggctccct gtaaatatcg atctgggtca cacaattact ttatcgtttc agcaccaatt
     2101 gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat aaggaataga
     2161 acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc gtggcaattg
     2221 ggattgcgtc cagtcttatt ctgattatgg tgatgaaaat cgcctcggta ttacagaatt
     2281 tgctctggca acgactgccg ggaactctgg ggatagccca ggattcacct ctctggatca
     2341 tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc agccagggtc
     2401 atgccggacc agaccccgcc tgtgaaccgc ttatcggctc accggttccg ccctctgcgc
     2461 taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc ctggggccgg
     2521 aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct cgtctgttac
     2581 cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaactatc ggcgcgctgt
     2641 ttggcacacc tgttgcggcg gcattgatat tttcgcaaac cttaaatggc agtaatgaag
     2701 ttccgctatg ggatcgtctc tttgcgccgt taatggcggc agcagctggt gcacttacta
     2761 ccggattatt tttccatcct catttttcac tgcccattgc gcattacgga cagatggaaa
     2821 tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca gggatggtcg
     2881 ccgtatggtg cttaccacgg ttgcacgcga tgatgcatca aatgaaaaat ccggtgttcg
     2941 tgctgggtat tggcggactt attctcggta ttctgggggt tattggtgga ccggtttcgc
     3001 tgtttaannn nnnnnnnctg gatgagatgc agcagatggt ggcaaatcag gctttcagca
     3061 ccagcgatta ctttttgctg gcggtgataa aacttgccgc cctggtcgtt gctgccgcca
     3121 gtggctttcg cggtgggcga atcttcccgg cagtgtttgt cggcgtggca ttagggttga
     3181 tgcttcatga gcacgttccc gccgttccag cggcaataac cgtttcctgc gctattctcg
     3241 gcatcgtgct ggtggtaaca cgcgatggct ggttaagtct ttttatggcg gcagtcgttg
     3301 tacccaatac cacattgcta ccgctgctct gtatcgtcat gctcccggca tggctgttat
     3361 tagcaggtaa gccgatgatg atggtcaatc gtcagaagca acagccaccc cacgataacg
     3421 tttagcaaaa aatgctctcc tttatgattt aagagttatg gcgatgatac ggcgctttac
     3481 ctccccgtaa tattgcgtta acaggccgct gacggcttcg atcgtgaagg agaataacaa
     3541 tgttcaggtc actgtttctg gcggccgccc tgatggcatt taccccgctt gcggcaaacg
     3601 caagtgaaat caccctactg ccatcaatca aattacaaat tggcgatcgc gatcattacg
     3661 gtaattactg ggacggtggt cactggcgcg accgtgacta ctggcaccgc aattatgaat
     3721 ggcgtaaaaa ccgctggtgg cgtcatgata gtggctacca tcgtggctgg gataagcgta
     3781 aagcgtatga gcgtggctat cgtgaaggct ggcgcgatcg tgacgatcat cgtggaaaag
     3841 gccgcggaca tggacaccgc cattaaacgc atccgtcaat ggagcacaat gcctgatgcg
     3901 acgctagcgc gtcttatcag gcctacaaac cagctcttta acgcgtaggg cggataaagc
     3961 atttacgccg catccgccaa tggtgccaaa tgcgacgctc attcaactac aatcccagtg
     4021 ccgttcctac cagtaaccag atattcagcg ccactaccag caccacaatc acccagcctg
     4081 tctgttttac gcgtttactg ttcaccagat cgcccatcaa cttgctgtca ctggtgaaaa
     4141 tcaacagtgg aaccagcgcc agggcgatac caaaacttaa cagcacctga ctcataacca
     4201 gaatccgtgt cggatctaat cccatcagaa tgacgataaa tgacggcagc atggtgactg
     4261 tacgacgcac ccacagcggg atatgaaagc gaatgaagcc ctgcat
//
LOCUS       DAWDRC010000345         4284 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8639_length_4284_cov_1.741783, whole genome shotgun sequence.
ACCESSION   DAWDRC010000345 DAWDRC010000000
VERSION     DAWDRC010000345.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8639_length_4284_cov_1.741783"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2028..2037
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3946..3955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1012 a         1163 c         1044 g         1045 t
ORIGIN      
        1 gagctaatac gaatagtttc aaagtcagat tctataagat atttccccaa cctccccctt
       61 gcaggcgtca ggagtagata acgcaaaatg ctctgtaccg cagcttctct ccgctacaga
      121 aagcgcaact atagccaccc acagccaggt tgattataat aaccgtttat ctgttcgtac
      181 tgtttactaa aacgacgaat cgcctgattt tcaggcacaa caagcatcaa caataaggat
      241 taaagctatg ggttttcttt ccggtaagcg cattctggta accggtgttg ccagcaaact
      301 gtccatcgcc tacggtatcg ctcaggcgat gcaccgcgaa ggagctgaac tggcattcac
      361 ctaccagaac gacaaactga aaggccgcgt agaagaattt gccgctcaat tgggttctga
      421 catcgttctg cagtgcgatg ttgccgaaga taccagcatc gacaccatgt tcgctgaact
      481 ggggaaagtt tggccgaaat ttgacggttt cgtacactct attggttttg cacctggcga
      541 tcagctggat ggtgactatg ttaacgccgt tacccgtgaa ggcttcaaaa ttgcccacga
      601 catcagctcc tacagcttcg ttgcaatggc aaaagcttgc cgctccatgc tgaatccggg
      661 ttctgccctg ctgacccttt cctaccttgg cgctgagcgc gctatcccga attacaacgt
      721 tatgggtctg gcaaaagcgt ctctggaagc gaacgtgcgc tatatggcga acgcgatggg
      781 tccggaaggt gtgcgtgtta acgccatctc tgctggtccg atccgtactc tggcggcttc
      841 cggtatcaaa gacttccgca aaatgctggc tcattgcgaa gccgttaccc cgattcgccg
      901 taccgttact attgaagatg tgggtaactc cgcggcattc ctgtgctccg atctctctgc
      961 cggtatctcc ggtgaagtgg tccacgttga cggcggtttc agcatcgctg caatgaacga
     1021 actcgaactg aaataatcgt tctgttggta aatattgggc ggcgttctgc cgcccgttat
     1081 ctctgttata cctttctgat atttgttatc gccgatccgt ctttctcccc ttctcgcctt
     1141 gcgtcaggat aacgatttcc tttacgacca aggagcgccc atggaacaac gccacatcac
     1201 cggcaaaagc cactggtatc atgaaacgca atccagtact gcggagtatg acgttctgcc
     1261 tctggtcccg gaagccgcaa aggtcagcga tccctttctg ctcgacgtga tccttgatga
     1321 agaaacgctg gcacccttcc tttcatggct ggtccctgcg cgcgttcttg cagtagagtt
     1381 gttccctgac cagcttaccg tgacccgttc acagaccttc accgcttatg aacgcttgtc
     1441 gacggccctg acggttgctc aggtttgcgg cgtccaacgg ttatgtaact actattcggc
     1501 gcgacttacg ccgctccccg ggcctgattc ctccagggaa agtaatcatc ggttggcaca
     1561 aatcacgcaa tatgcccgcc aactggctag ctcgccttct attatcaaca accgatcgcg
     1621 ccagcatctg aatgacgttg gtcttactgt ctgtgactgt gtgatcatta accaaatcat
     1681 tggttttatt ggctttcagg cgcggacaat tgcgacattt caggcttatc tcgggcatcc
     1741 ggtacgctgg ttacccggtc tggagataca aaactacgcc gacgcgtcac tgtttgctga
     1801 tgaatcaata cgctggcgaa gcagctatga ggtggaaaaa ctccctgaag agcacacaaa
     1861 aagttcaact gcagaacttt gccaactggc caacacactc tctctccacc ctatttcact
     1921 ttcccttctc gaaaagttgt taaacagcac acgggtcaat acacagccgg ataatcagct
     1981 tgcggcgttg ttatgcgcgc ggataaattg caatcctgcc tgtttttnnn nnnnnnnctg
     2041 ctgcgcgcgc taccgtgcag gcgttacaat ggctgacccg agcacccgat cgctttagcg
     2101 ccgcccagtt cagcccatta ctcgaacacg aaaaatcatc aacgcagatt attaatctgc
     2161 tggtatggag cgggctgtgt ggctggataa atcgcttaaa aatcgcgttg ggtgagacat
     2221 attaaccttg ccgcgtcaga cagattcgcg taaaactgtc agccgctcta atggccacca
     2281 aaatagacaa ttatgtttca ggacaacccg ctgctagcgc agcttaaaca gcaactgcat
     2341 tcccagacgc cacgcgctga aggggtggta aaagccacag aaaaaggctt tggcttcctg
     2401 gaagtcgacg cgcaaaaaag ttatttcatt ccgccgccgc agatgaaaaa agtcatgcat
     2461 ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac gtgaatccgc agagccagaa
     2521 gaactggttg aaccgttcct gactcgtttc gtgggtaaag ttcagggcaa aaatgaccgt
     2581 ctggccatcg ttcctgatca tccactctta aaagacgcca ttccttgccg cgcagcccgt
     2641 ggcctgaacc acgagtttaa agaaggcgac tgggcggttg ccgaaatgcg ccgtcatccg
     2701 ctgaaaggcg atcgttcttt ctatgctgaa ctgacacaat acatcacttt tggtgacgat
     2761 cactttgtac cgtggtgggt tacccttgcg cgtcataacc tggaaaaaga agcaccagac
     2821 ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg aagatctgac tgcgctggat
     2881 tttgtcacca tcgacagtgc cagcacagaa gatatggatg acgccctttt cgccaaggcg
     2941 ttaccggatg gcaaacttca gctgattgtg gcgattgccg atccaaccgc gtggattgct
     3001 gaaggcagca agctggacaa agccgcgaaa attcgcgcat tcaccaacta tctgcctggc
     3061 ttcaacatcc ctatgctgcc tcgcgagctt tctgacgatc tctgctcact gcgcgccaat
     3121 gaagtccgcc cggtactggc atgccgcatg acgctctcca ctgatggcac cattgaagat
     3181 aatatcgaat tctttgccgc caccatcgaa tccaaagcga agctggtgta tgaccaggtt
     3241 tctgactggc tggaaaatac cggtgactgg cagcctgaaa gcgaagcaat tgccgaacaa
     3301 gtccgtttgc tagcacaaat ttgccaacgc cgcggcgagt ggcgtcataa ccacgcactg
     3361 gtgtttaaag atcgcccgga ttaccgcttt attctcggtg aaaaaggtga agtgctggat
     3421 atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg aagaagcgat gattgccgct
     3481 aacatttgtg cggcccgcgt actgcgcgat aagctcggtt ttggcatcta taacgtgcat
     3541 atgggctttg atccggcgaa tgccgacgcg ttggcagcgt tgctgaaaac gcacggtctg
     3601 catgtcgatg ctgaagaagt gctcacgctg gacggtttct gcaaactgcg tcgtgaactg
     3661 gatgcgcaac caactggttt cctcgacagc cgcattcgtc gcttccagtc atttgctgaa
     3721 attagcactg aacccggtcc tcactttggc ctcggtctgg aagcatacgc cacctggact
     3781 tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc tgctgaaagc ggttatcaaa
     3841 ggcgaaactg cgacgcgtcc acaggatgaa atcactgtcc aaatggccga gcgtcgccgt
     3901 ctcaaccgga tggcagaacg tgatgttggc gactggttat acgcannnnn nnnnncgtgt
     3961 tcgtttggtt gataacggcg ctatcgcctt tattccagca cctttcttac acgctgtgcg
     4021 cgatgaactg gtttgcagcc aggaaaacgg cactgtgcaa attaaaggtg aaacggctta
     4081 caaagtgact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa cccgcagcat
     4141 aattgcgcgc ccagtcgcgt aatctccttt cacggcccat tcctcatgga tgggccgttt
     4201 atttccccgc tcaaccttcg tcatatcccg gcagattttt aactgacttt cgtttgaaaa
     4261 ctggcgtttt tccaaaactg gatt
//
LOCUS       DAWDRC010000346         4280 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8647_length_4280_cov_1.654675, whole genome shotgun sequence.
ACCESSION   DAWDRC010000346 DAWDRC010000000
VERSION     DAWDRC010000346.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8647_length_4280_cov_1.654675"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1776..1785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1000 a         1143 c         1174 g          953 t
ORIGIN      
        1 gagtgcgagc atcggtgaac agggtgctaa gcgcacctgg gctaacggct tcgttcatag
       61 caattcctta cagagcggct tcacggtgat gtaacaggct ggcaagcccg ttgagtaaca
      121 gagcattaaa cgtttcggga tcggtcatgt tgcaggcgtg tccgccatag cgcatcacca
      181 ttttctggct atcgggcaga gcggcatgaa gttcactgga acatgctgat ggcaccagca
      241 gatcatcact ggcgcagatg atttgcaccg ggcagcggat acgatccgca tggcgactaa
      301 agtcagcgcg tttgagggcg ttaagtcgac gcagtaaatt atttttgccc tgaaaatgcg
      361 ccagtgccag cgcgtcttct gcctcaaggc gaggtgcgcg ggccgccatc cagtcggcgg
      421 gatagaggaa caacggctgc gcttccaccc atgcctgcgc gccgccgcta tgtagtaact
      481 gttcgcgaac ctgaaaacag cggcgcgtat gggcgtttat tcgtagccag ccgttaacgc
      541 tgaccagcac agttaccgac gcgggataat ccagcgccag ctgcattccc accagcgcac
      601 cgagcgcatg gccgaccacc gcgtaacgct caatccctgc ggctaccagc gcctgatgca
      661 gttccgctgc catctgggcg atactgtaat cttctgccag cgtgtcggga ttattgccgg
      721 tgccgcgctg gtcgtaacag actacctgat actcctgatc cagcaccgcc agttgcggta
      781 accagtaact gccgctaccg ccaagacccg aaatcaacac cactacgggc gcatcagcat
      841 aagggggagg tgagagtgaa agtttcatcg cggcctcact tggcgatatg cgcaattgtg
      901 gcgatttcca ccagcgcgtc aggttttact agtccgcact gaatgcagaa tcgcgccggt
      961 ttgtcacccg ggaaaaactc ggcgtagatt tcgttaatcg cggcgtaatt tttccagtcg
     1021 gtaataaaga tgctgttgaa ggtcacatcc gccatcgtgc cacccgccgt ctcgatgacc
     1081 ttgcggatgg tttccagaac gtggcgggtt tgcgcctttg ggtcatcggc aaacagcacg
     1141 ttgttatgtt gatcaaaagc cagcgtaccg gagacataca ccacgccatc agccagcgtg
     1201 ccgggaacga agggggccag cggtgcgctg ctgccagcag gaataattac ggattttggc
     1261 atcgttaaac tccttaagcg atacgagcaa aggacgtgga agaaagcgcg tcgcagaacg
     1321 tttcgacgtc gctgacccag ccaaaaaagg tttcgatatt gaacaacgcg gctttctgtg
     1381 caaattccgg ccccgcctgg tgtgttgcgt cttccagcac cacgccgaaa tactccagaa
     1441 aaaagccgtc gcgtagcgtc gattcgacgc agacgttggt agcgataccg gtgaaaacca
     1501 gatggcgtat tccacggctg cgcaaaatgc tgtccagcgg cgtattgaag aaaccgctgt
     1561 agcgcggctt cggcagcaca atatcgccag gctgcggcac cagttcatcc accagttgat
     1621 aatcccagga gcctttcgcc agcaatttcc cctgcagctg cggctgctta cgcatggttt
     1681 tcagggcgtt cgatttatga aaattcggtg agccggggcc gccagcctcg acatactgtt
     1741 catcccagcc attttgaaac cagatgatca acatcnnnnn nnnnncccgg cgagatctaa
     1801 gtagccgcct ggcgtggcat aagcgttttg catatccacc acgatcagcg cagtttgctg
     1861 cggatcgaag gtaatggctt ccggtcgtgc ggttaaggtc gtcatcatgc cacctcctga
     1921 gtcagcgcag ggagatgggc gcggcactgc atcagtggtt gaatgcgctc gccgaagttt
     1981 tcgattcccg acagaaaatc atcgaaggtc aacagcacgc cttcggcacc aggcacgctt
     2041 gcgacttcat ctaacatgcg cgcgacactg gcgtaagaac cgactaacgt ccccatattg
     2101 atgtttaccg ccgaagtggg atcggccatc tgacggacgt tggtgtcagt atcggagcgg
     2161 gtatctttct gactttgttc ggttagccag cttaacgcct cttcatccgc gcccgctttg
     2221 tagtgttccc attttgcacg agcggcatcg tcggtttcgt cggcaatcac cataaacaac
     2281 acataagagc caacgtcgcg tccggtttgc tctgcggcct gtttcatccg cgcagcggtc
     2341 ggggcgaaag ccgtgggtgt atttacgcct ttgccgaaac agaagttaaa atcggcatac
     2401 cgggcggaga acgccatgcc agcgtcgctt tgcccggcgc agatcacttt catggggaca
     2461 ctcggttgcg gactgacgcg acaatcatcc atagtgaaaa aatcgccttt aaaatcgctt
     2521 ttccccgagc cccacaggtc gcgcagcacc tgaacatatt cggtgagata gtcgtaacga
     2581 cgggagaaat agtcatcgcc aggccagatc cccatctgct catactcggg cttctgccag
     2641 ccagtcacga ggttgacgcc aaaacgcccg ccagagatgg agtcgatggt tgcggccata
     2701 cgggcgacga ttgccggagg taacgttaag gtggcagctg tcgcgtatat ctgaatgcgt
     2761 gaggtgacgg ctgccagccc cgccatcaag gtgaacgact caaggttgtg atcccagaac
     2821 tcagttttgc cgccaaagcc acgcagtttg atcatcgaca gggcgaaatc gaaatggtag
     2881 tgctccgctt tttgcacaat ggctttattc agttcaaagg tcggcatgta ctgcggcgcg
     2941 tgggtcgaaa tgagccagcc gttgttgcca ataggtacga atacgccaat tttcatcatc
     3001 aacctctctt cgtctcgtaa agtgaaagtc agacggggcg ctgcatcctg catatccttt
     3061 tcagccgcgt aatggcttgt ttgcaaagcg gatgccagtt tttaaaaagt gaatgttatt
     3121 aatgtgttaa tgttaagttg tctaaaatgc ctgaaaaaaa gtggactaaa cggtcaaaac
     3181 agttgcacat aaaacatgca ttcgtgcgcg atgagagtgc agaaggtcga ggccgggcgg
     3241 gggttttgct atcctgttgc caatcaacaa gaggggagag cgcatgacgc aaggcgcagt
     3301 gaaaacaacg ggtaagcgtt cgcgcgcagt aagcgcgaag aaaaaagcga ttcttagcgc
     3361 agcactggac actttttcac aattcggttt tcacggcaca aggctggagc agatcgcgga
     3421 gttggcgggt gtttcaaaaa ccaatctgct gtattacttt ccgtcaaaag aggcgctgta
     3481 tatagccgta ctgcggcaga ttctcgatat ctggctggca ccgttaaaag cgtttcgaga
     3541 agatttcgcc ccgctggcgg cgatcaaaga atacatccgt ctgaagctgg aagtttcccg
     3601 cgattatccg caggcttcgc gcctgttctg tatggagatg ctggcaggtg caccgctgtt
     3661 aatggatgaa ctgacgggcg atttgaaggc attaattgat gagaaatcgg cgctcattgc
     3721 cggttgggtt aaaagcggca aactcgcgcc gattgacccg cagcatttga tttttatgat
     3781 ttgggcttcc actcaacatt acgccgattt tgcccctcag gtggaggcgg tgacaggcgc
     3841 gacgttgcgc gatgaggtgt ttttcaatca aacggttgaa aacgtgcagc ggattattat
     3901 tgaggggatt cgaccacgtt aaagatgccg gaggaggttg taacatcctc cggctacctg
     3961 tttaaccgat cgtcattaaa ctggcgttac cgccagcagc agcggtattc acactcagcg
     4021 aacgctcgat atacagccgt tccagaagga tattgctttc gccacgggca aaaccctgca
     4081 ccgaaacaat tgcaccatcc cgcgcggcaa cggcttcaca caatgcgcga agctgatccg
     4141 aatcaccgtg gaagatcacc gcatcaaatg gttgagcggt tatattttcc gctttcgcca
     4201 gttgaatacg ttcgctgact gccgatggca atgccttcac taactgacga tgcagcgcat
     4261 catccggcca cagcacctgg
//
LOCUS       DAWDRC010000347         4280 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8646_length_4280_cov_1.854911, whole genome shotgun sequence.
ACCESSION   DAWDRC010000347 DAWDRC010000000
VERSION     DAWDRC010000347.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8646_length_4280_cov_1.854911"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    291..300
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1632..1641
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2190..2199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1034 a          980 c         1095 g         1141 t
ORIGIN      
        1 aaacaggctg gcctgaatac gtgtgctttt tttctgatgg tcaatcacca tcagcgtttc
       61 agcgagataa aaacagaaat cagggcagtt attttccgct gacagttgcg gtaaattttc
      121 aaatcccgcc acaaggtcat aagagaacag gccgccgaag aacattgctt ctcgttcttc
      181 cttcggtaca ttcaacagat tctgtaataa gcggaaagcg tcaaaaaccg aaagggagca
      241 taagcgggcg tcttcatcca gcagtggact gacaggcggg aagcgcagta nnnnnnnnnn
      301 cggcagtttg gtgattgttc attttccaca cccgcaggca aggcgttatc cagtagtgtc
      361 aacagggctt ctccattgcc ggaaagcgcc tggattgtga cagtgtcact taatgctgta
      421 atgcgcagcg cactgtctac cagcagcagg ctttttaaat catctttgct gtcgatatct
      481 gcggattcca gcagcagcgt tgccggacga tccccacaca actggtgaaa aagcgcagtc
      541 gggttgtcgc gataagcgcc tttgcaggtt agcagttcga gagtcggttt ttgtgtttgc
      601 attgttattc tctaattttg ttcaaaaaaa agcccgctca ttaggcgggc tgggtatctg
      661 attgctttac gcatggtgaa tacactgccc gtttcaggaa gtgcgccacc aacctttcag
      721 tacgaaaatt gctttcattg gcgataccct ttttacgtga acttgcgtac tagttaacta
      781 gttcgatgat taattgtcaa cagctcattt cagaatattt gccagaaccg ttatgatgtc
      841 ggcgcaaaaa acattatcca gaacgggagt gcgccttgag cgacacgaat tacgcagtga
      901 tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca gaagcattgg
      961 tgcaccgtgc agtcgagatg cgcgtcggca ccctggcgat caccgaccat gacaccacag
     1021 ctgcaattgc accagcaaga gaggaaattt cacgttcggg actggcgctg aatcttattc
     1081 ctggtgtgga aatttccacg gtctgggaaa atcatgaaat tcatattgtc gggctgaata
     1141 ttgatattac tcatccgcta atgtgtgagt tcctcgcgca gcagacagaa cggcgcaatc
     1201 agcgggcgca gctgattgcc gaaagacttg aaaaagcgca aatacctggg gcactggaag
     1261 gcgcgcaacg actggcgcag ggcggtgcgg tgacgcgcgg tcattttgcg cgtttccttg
     1321 tggagtgcgg aaaagccagt tcaatggcgg atgtctttaa aaagtatctg gcgcgcggga
     1381 aaaccggata cgttccgcca cagtggtgta caatagagca atctattgat gtcattcatc
     1441 attctggcgg taaggcggtg ctagctcatc ctgggcggta caatcttagt gctaaatggc
     1501 tgaaaagatt ggttgcgcat tttgccgaac accacggcga cgcgatggaa gtcgcgcagt
     1561 gccagcaatc gcccaatgaa cgcacccagc tggcgacact tgcgcgtcag catcctttat
     1621 gggcatcaca annnnnnnnn nctgattttc atcagccatg cccgtggatc gaactgggtc
     1681 gtaaactctg gctgcccgcg ggcgttgaag gcgtctggca gctatgggaa cagccgcaga
     1741 acaccacaga gagggaatta tgagccagtt tttttatatt catcctgata acccgcagca
     1801 acgcctgatc aaccaggcgg tggagatcgt gcgcaaaggc ggggtgattg tttatccaac
     1861 tgattccggc tatgcgctcg gctgtaaaat tgaagataaa aacgcgatgg agcgtatttg
     1921 tcgtattcgc cagctgccgg acggtcacaa ctttaccctg atgtgtcgcg atctttctga
     1981 actctcgacc tattcatttg tcgataacgt tgcgtttcgt ttgatgaaaa acaacacgcc
     2041 aggcaactat accttcatcc tgaaggggac gaaggaggtg ccacgccgtc tgttgcagga
     2101 aaaacgcaaa accatcggta tgcgcgtgcc gtctaacccg attgcgcagg cgctgcttga
     2161 agcactgggc gaaccgatgc tttccacttn nnnnnnnnnt tccacttcgc taatgctgcc
     2221 aggcagtgaa tttaccgaat ccgatccgga agaaattaaa gaccgtctgg aaaaacaggt
     2281 ggatttgatt attcacggcg gctatctcgg gcagaaacca acaacggtta ttgatctcac
     2341 cgatgatacg ccggtcgtgg tgcgtgaagg cgtaggtgat gtgaagcctt tcttataaca
     2401 aattgcgttc cacggatgga aaatgatggc agggatatat cgctgcattt tgctgttgat
     2461 agttgggctt tttttctctt cgttgagtta tgcgaaaaac acggagatcc cttcttatga
     2521 agaagggatc tcgctctttg atgttgaagc cactctgcaa ccgaatgggg tgctcgacat
     2581 caaagagaac attcattttc aggcgcgaaa tcagcagatt aggcacggat tttatcgtga
     2641 tttaccacga ctctggatgc agcctgatgg ggacgctgca ctgctgaact atcatattgt
     2701 tggcgtcacc cgtgatggta ttcctgaacc ctggcatctt gactggcata tcgggttaat
     2761 gagtattgtc gtgggcgata aacaacgttt cttgcctcaa ggcgactatc attatcaaat
     2821 tcattatcag gttaaaaatg ctttcctgcg tgagggggat tctgatctgc taatctggaa
     2881 cgtgaccggt aaccactggc cgtttgaaat atataagacc ctattttcac tcaagttgcc
     2941 tgatattgcg ggtaatccat ttagcgaaat cgatctcttt actggagaag agggcgacac
     3001 atatcgaaat ggccgcatcc ttgaggacgg aagaattgaa tctcgcgatc cgttttatcg
     3061 tgaagatttc acggtcctct accgctggcc tcacgcatta cttggtaatg ccccggctcc
     3121 acaaacgacg aatattttca gtcatcttct tttaccctct acgtcatcgt tgttaatttg
     3181 gtttccgtgt ctgttcctgg tgtgtggatg gttatatctc tggaagcgca ggccgcaatt
     3241 tacgccggta gatgtgattg aaaccgatgt cattccgcca gattacacgc ccggcatgtt
     3301 acgtctcgat gcgaagctgg tttacgacga taaaggtttt tgtgccgata tcgtaaatct
     3361 gatcgtgaag ggaaaaattc atctggaaga tcagtctgac aagaaccagc aaaccctgat
     3421 tcgtgttaat gaaggtgcca caagaaataa tgcggtatta ctacccgcag agcagttttt
     3481 actggaagcg ttatttcgta aaggcgataa gatcgttctt acggggaggc gcaacagagt
     3541 cttacgcaga gcatttttac ggatgcagaa attttatctg ccgcgtaaaa agtcttcgtt
     3601 ttaccgacct gatgcgtttt tgcaatgggg cggaatggca atattggcgg tcattctcta
     3661 cggtaatctg agtcccgtag gttcggcagg aatgagtctg gtggacgata tgtttattat
     3721 gatctgctgg cttcttcctt ttttattttg ttcccttgag cttttgtttg cccgcgatga
     3781 tgacaagcct tgcgttaatc gtgtcatcat cactttgttt ttaccactga tttgttcagg
     3841 cgtggccttc tattctctct acatcaatgt cggagatgta ttcttttact ggtatatgcc
     3901 agcgggttat tttagcgcgg ttttcctgac cggttatctc actggcatgg ggtatatttt
     3961 tctgccaaag tttacccaaa ctgggcagca acgttatgcc cacggtgaag ctatcgttaa
     4021 ctattttgcg cgtaaagagg cggcaacaca cagtgggcgg cgtaggaaag gggaaacacg
     4081 gaaactggat tacgctttgt taggttgggc agtctcggca aaccttggca gagaatgggc
     4141 agcacgtatc accccatcac tcacagcggc tgttcgcgcc ccggaaattg cccgtagtgg
     4201 cgttttgttc tcattacaga tgcatctgag tctgggggcc aataccagtt tgttggggcg
     4261 aagttattcc ggtggtggtg
//
LOCUS       DAWDRC010000348         4268 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8680_length_4268_cov_1.876335, whole genome shotgun sequence.
ACCESSION   DAWDRC010000348 DAWDRC010000000
VERSION     DAWDRC010000348.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8680_length_4268_cov_1.876335"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1819..1828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3101..3110
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3622..3631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1009 a         1163 c         1097 g          969 t
ORIGIN      
        1 gtgccgtcag tcaaatagcc accagctttc atctccatcg ccacaaagcc cagcacgctg
       61 ttattaaaga cgataatttt cactggcagc ttcatttgca ctaccgagag gaaatcgccc
      121 atcaacatgc taaaaccgcc atcgccgcac atggcgacca cctggcgttc tggctcggtt
      181 gcctgcgcgc ccagcgcctg cggcctggcg ttagccatcg aaccgtggtt aaacgaacct
      241 aacaggcgac gcttgccgtt catttttaga taacgtgccg cccacaccgt tggcgtacca
      301 acgtcacagg tgaaaatagc gtcatcggcg gcaaaatgac taatttgctg cgccagatat
      361 tgcgggtgaa tggctttctc gctcggttta gctaaatcgt ccaacccttt gcgggcgtcg
      421 cggtaatctt ccagtgcctt atccagaaac ttgcgttcgg ttttttcttc caccagtggc
      481 agcaatgcgc gcagggttga tttgatatcg ccgaccagcg ccatatctac cttgctatgc
      541 gcgccgatgc tggctgggtt gatatcaatc tgaataattt tggcatcggt cgggtagaac
      601 gcacggtagg gaaactgagt accgagcagc actaacgtat cggcgttcat catggtatgg
      661 aaacctgacg aaaagccgat taatcccgtc attccaacat catatggatt atcgtattcg
      721 acatgttctt taccgcgcag ggcatggact ataggcgctt taattttccc ggcaaactca
      781 actaactctt tatgcgcccc cgcacagccg ctgccacaca tcagggcgat attgctggaa
      841 taacgcagca gttgcgccag tttgcgtaac tcttcttctt ccggtgttac taccggctgt
      901 ggcgcatgat accagtgggt agttgcccct tctggcgcag gttttaacgc cacgtcgccc
      961 ggtaacacaa caacggaaac gccacggtta agcaccgctt tgcgcatagc aattgccagc
     1021 acttgtggga tttgctccgg gctggaaaca agctcgcaat agtgactaca ttcgcggaat
     1081 agctcttgtg ggtgggtttc ctggaaatag ccgctgccaa tttcgctgga gggaatatga
     1141 gcggcaatcg ccagtaccgg aacgtgattg cggtggcaat cgaacaggcc gttgattaag
     1201 tgcaggttgc cgggaccgca cgatccggca cagaccgcca gttctccgct aagttgtgct
     1261 tcagcgccag cggcaaacgc cgccacttct tcatgccggg tggacatcca ctcgatggtg
     1321 cccatgcgat taagactgtc actaagaccg ttcagagagt cgcccgtgac tccccagatg
     1381 cgtttcacgc ctgccgattc gagtgttttg gcgatataag ctgcaaccgt ttgtttcatg
     1441 gttctccatc tcctgaatgt gataacggta acaagtttag ttcatctgac ggagggggaa
     1501 gggatgggag aaaaaggagg cactaacggt taaatagccc gatgaaagga atatcatcgg
     1561 gcatcaggcg attatgcgag aaccaaatcc ccctgcggat ggcaggagca ggccagtacg
     1621 taaccttcag cgatttcggc gtcggtcagc gtcattgtgc tgctcaccgt atattcaccg
     1681 gaaatcactt tcgtcttaca gcagccgcaa acacccgcac gacaggcggc gacaaccgga
     1741 acgttattgc tttccagcgc ctccagtagc gtggtgccaa ccggagcgta aaattctcgt
     1801 gccggttgca gtttggtgnn nnnnnnnncg ctggtcgccg cttccgctac tggggtgaag
     1861 aatttctctt taaagaaacg cgtcacgccg agcgctttca cttcctgctc tacccaatcc
     1921 atatatggag cagggccaca ggtcattacg gtacgtgagg ctaaatcagg gacacctgcc
     1981 agcagttcgc gagtgagacg accagcgata aagccttcgg taacgttatt ttccgccacc
     2041 agcgttaccg gatagttacg ccactcatcg gcgaaaatca catcctgcgg cgtacgcacg
     2101 ttgtagatca cctgcacatc ggcctgtgga cggttcttcg ccagccagcg acgcatcgac
     2161 ataatcggcg taacgccgca gcctgccgcc agcaacagga atttatcttc tgctttatcg
     2221 tcgcaggtaa attcccccat cgcgtccgaa agccagagat aatcaccgcg ttttacatcg
     2281 cgcgtcagcc accgggagcc gacaccgtca tcaatccgcc gcacagtcag ggtgatgtat
     2341 tcactcacgc ctggcgtgga ggaaatggtg taagcacgca gcgtttccgc tgagttacgc
     2401 acgctgacca gcgcatattg cccggcgcga tacgggtagt aatcgtggca aatcagggaa
     2461 atcgtccaca catccggcgt ttcttgcgta atgtgatgaa cctgcatccg ccacgggcat
     2521 tgattcgttg gcatcgtcat cgacaaactc cttacgcgct caacagttgc ttcatgtctt
     2581 cttcaacagt ggtgatagaa cgcaggccga atttctcgtt cagcaccgcc agcaggtctg
     2641 gtgtcaggaa accaggtgca gtcgggccgg tgacgatatt tttcacaccc agagaaagca
     2701 gcgtcagcag aatgacaatc gctttctgtt caaaccagga gagcaccagc gacagcggca
     2761 gatcgttcac cccacagccc agtttctctg ccagcgttac cgccagaata attgctgagt
     2821 aagcatcgtt acactgacca gcatctacca gacgcggcag accttcgata tcgccaaact
     2881 caagtttatt aaagcgatat ttaccgcaag cgagggtcag gatcaggcag tcatccggca
     2941 cgctggtggc gaaatcggtg aagtagtgac gctcgccgcg tgcgccgtca cagccgccaa
     3001 gcaggaagat atgacgcagt ttttcacggc tcaccagatc aatcagcgta tcggctgcgc
     3061 caagcagtgt ctggcgacca aagcccacgg tgatcaggtg nnnnnnnnnn aactccactt
     3121 gctgattctg ccagccgctg ccgtagttac cgaccagatg cttgaattta cgcagctccg
     3181 ggtaaccatg cgcaggcagc atttcgccgt gggtgtagac attaacgccc gtgccttcgg
     3241 tctgttccag caggttgtag agatctttga gatcgtgacc ggaaatcaga atgcatttac
     3301 ccgccgtcgc tttgacgttg acctgagttg gcgtcgggtg accgtattta ccggtttcgc
     3361 ctgcatccag aatgctcatc actttgaaat tcatctggcc gatttccatt gaacactcaa
     3421 gaagcgcgtt catatcggca ggccaggtcc ccagccacgc catgatttta tggtactggg
     3481 cataaatatc gttgtcgtat tgaccgagaa catgcgcgtg ttccatatag gccgccgcac
     3541 ctttcaggcc atacaggcac agcagacgca ggccgagaat gttttcgcca atcgccgctt
     3601 tatctttgtt aggggtaaat tnnnnnnnnn ntacagccag gcattgtgct ttcagcgcct
     3661 cgcgcagggc aatcgcttca cgagcgtagc cgacaatacg cggagaatcg aagttaacgt
     3721 tggtcagggt tgagaaaaag gcacgtggcg caaagctgtc tacatcgtgg ttgatgatgc
     3781 cgtattcacg tgctttcact gcccaggccg aaagcccttg cagcgccgcg atgagtaaat
     3841 cctgaaggtc agaggtttcc gccgttttac cacacatccc ctgcgcgtat gagcagccgt
     3901 ttcctgccgg agtacggata gtttgttcac attgcacaca aaacatgatc acacctttta
     3961 aagttatatt taatatacat gtttaaggtt aagacgctta acgcggggat aaaagagatt
     4021 tttaatgcaa ctttaaggga gattgattta gcgcaatttt ggcggcagag atctaccgcc
     4081 agagaggtat tatgcagaga aaaaggcgat gagaatcggc actaacagac taagaataaa
     4141 accgtgaaca atcgccgccg ggaccatatc cagcccgcct gtacgttgaa gaacgggcag
     4201 ggtgaaatcc atcgacgtcg caccgcataa gcccagtgca gtagagcggc tacggcgaat
     4261 cagcccag
//
LOCUS       DAWDRC010000349         4265 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8687_length_4265_cov_2.747031, whole genome shotgun sequence.
ACCESSION   DAWDRC010000349 DAWDRC010000000
VERSION     DAWDRC010000349.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8687_length_4265_cov_2.747031"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1441..1450
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1100 a         1061 c          951 g         1143 t
ORIGIN      
        1 tgatggtgat accacgagct ttttcttccg gcgcgttatc gatctggtcg aatgcgcgag
       61 cagcaccgcc gtaggtttta gccagtacgg tagtgattgc agcggtcagc gttgttttac
      121 catggtcaac gtggccgata gtaccgacgt taacgtgcgg ttttgtacgt tcaaactttt
      181 ctttagacat cgattgtccc tctaagacac ggataaatcg gtgatatcac cacatcaacc
      241 aggcaacatg cccgacttgt tgaatgcaat aaacagaagg aaaaaacagg gaggagaaaa
      301 gaagtggtgc tgataggcag attcgaactg ccgacctcac ccttaccaag ggtgcgctct
      361 accaactgag ctatatcagc acatcttgga gcgggcagcg ggaatcgaac ccgcatcatc
      421 agcttggaag gctgaggtaa tagccattat acgatgcccg catcctggaa ctcggctacc
      481 tgattttcat tctgcactga atatcgagag aagctctctt tattcgagcc ggtaagcgaa
      541 cttatcgtct cgggctacgc catcgcgtgg ccgaaattgg tggtggggga aggattcgaa
      601 ccttcgaagt ctgtgacggc agatttacag tctgctccct ttggccgctc gggaacccca
      661 ccggacttga tggtgccgac taccggaatc gaactggtga cctactgatt acaagtcagt
      721 tgctctacct actgagctaa gtcggcatca agtagcgcgc attctatgga gacatgcgag
      781 ttcatgcaac taaaaaattg cataatttgt tttattggtc acattttatg cgacacgatg
      841 aagaaacagc cgaacggagg gcagattagc acactttttc aacatcattg tgctcaacaa
      901 tgtgctcctg ctaaaccata attcttttta tcagatggaa tatctgtcac attgcttttc
      961 aacgatagct tcctgggaga gattttttct tattattcct ccccatctgg tgttaccctc
     1021 ctgcccatta acccattcaa cagaactgtg acgcgccatg gcaaatatcg ctttgccgat
     1081 agagctatga ccgccagaaa catgcttatg agtataaaag agcaaacgtt aatgacgcct
     1141 tacctacagt ttgaccgcaa ccagtgggca gctctgcgtg attccgtacc tatgacgtta
     1201 tcggaagatg agatcgcccg tctcaaaggt attaatgaag atctctcgtt agaagaagtt
     1261 gccgagatct atttacctct gtcacgtttg ctgaacttct atataagctc gaatctgcgc
     1321 cgtcaggcag ttctggaaca gtttcttggt accaacgggc aacgcattcc ttacattatc
     1381 agtattgctg gtagtgtcgc ggtggggaaa agtacaaccg cccgtgtatt gcaggcgcta
     1441 nnnnnnnnnn ggcgctatta agccgttggc cggaacatcg tcgtgttgaa ctgatcacta
     1501 cagatggctt ccttcaccct aatcaggttc tgaaagaacg tggtctgatg aagaagaaag
     1561 gcttcccgga atcgtatgat atgcatcgcc tggtgaagtt tgtttccgat ctcaaatccg
     1621 gcgtgccaaa cgttacagcc cctgtttact cgcatcttat ttatgatgtg atcccggatg
     1681 gagataaaac tgttgttcag cctgatattt taattcttga agggttaaat gtcttacaga
     1741 gcgggatgga ttatccacac gatccacatc atgtatttgt ttctgatttt gtcgattttt
     1801 cgatatatgt tgatgcaccg gaagacttac ttcagacgtg gtatatcaac cgttttctga
     1861 aattccgcga aggggctttt acagacccgg attcctattt tcataactac gcgaaattaa
     1921 ctaaagaaga agctattaag actgccatga cattgtggaa agagatcaac tggctgaact
     1981 taaagcaaaa tattctacct actcgcgagc gtgccagttt gatcctgacg aaaagtgcta
     2041 atcatgcagt agaagaggtc agactacgca aataattttc aggggagcta atgctcccct
     2101 ttcttatttt tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc
     2161 atcctgctca agtaataaag caccctgttt gtctattccg cgtgaaatgc caaatatttc
     2221 tttatcacca atgataagtt tcactgggcg attaataaaa ttatccaact tttcccagcg
     2281 cgaaagataa ggtgtcaatc cttcttgctc gaagagttcc agcgccgcgc gtaattcacg
     2341 tatcagcatg gccgcaagag tattacgatc gagattgatc ccggcttcct gcagcgtgat
     2401 ccacccttga ttaacgacac tctcttcaac acgacgcatt gccatgttga tccctgcacc
     2461 aatgactatt tgcgccgcat cgccggtttt gccagtcagc tccacaagaa tgcctgccag
     2521 cttacgatcc tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag
     2581 cttgcgtaat acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc
     2641 cgggccctgt tccagacgcc agaacatcga caaatataag ttcgcgccaa aaggcgaaaa
     2701 ccatttccga ccacgacgac cacggccagc atgctggtat tctgcaacac aggcatcgcc
     2761 agatttaagc tctccgatgc ggtccaggag atactgattc gtggagtcaa taactggcag
     2821 cacggctaca ctgccgtcat ccagctgact caatatcttt tcggcattaa gtaactggat
     2881 tggctcgggc aagctgtatc ctttacccgg aacagtaaat acatcaacgc cccagtcacg
     2941 cagtgtctga atgtgtttat taatagccgc ccggctcatt cccagcgttt cacccaactg
     3001 ctcgccagag tgaaattcac cgttcgctaa cagggcgatc aatttcagtg gcacggtgtt
     3061 atccttcatg aaattgtctc cactgcgctc acttcacctg atgcaccaat aaagcggact
     3121 tcaggctcaa gccagacatt aaatttttca cccacttttt gtcttacatg atgcgccagc
     3181 tgtacaacat cttcgctttt tgcatcgtgc tcatttatga gaaccagcgc ctgttgacgg
     3241 tgtaccgcag ccccaccaat ttgcgtccct ttaagctggc actgatcaat cagccaaccc
     3301 gcggccagtt ttactgaacc atccacctgg gggtaatgtg gggctgttgg aaattgcgcc
     3361 aacaacgcgt tagcagtttc ggcagaaaca acaggatttt tgaagaaact acccgcatta
     3421 ccgttcactt ttggatcagg gagtttggtg gtgcgcatat gacacaccgc atcaaatact
     3481 tgctgtggcg ttactgttgt aggatccaga cgagttaagt caccatacgt tagtacaggt
     3541 tgccactctt ttggcagacg cagacctacg gctacaatgg cgaagcgatc ctggtattca
     3601 tgtttaaaaa tactgtcgcg atagccaaaa cggcactctt tggcagttaa gcgtacttgc
     3661 ttgcctgtcg ccagttcaac acaatcaaca taagcgcaaa ctcgctgtaa ttctacgcca
     3721 taagcaccaa tattctggat aggtgatgag ccgacacaac caggaattaa tgccagattt
     3781 tccagaccag gcataccttc ctgcaaagtg tattttacca ggcgatgcca gttttctccg
     3841 gctcctacat gtaaatacca cgcatcaggt tcatcatgaa tttcgatacc tttgatccgg
     3901 ttgatgatca ccgtgccgcg atagtcctcc agaaaaagta cattacttcc ttcacccaga
     3961 ataagaacgg gttgtccttc tgcggttgca tgctgccagg cattgagtaa ttgttgttcg
     4021 tcttcggcac atacaatgtg ctgagcatta tgatcaatgc caaatgtgtt ccagggtttt
     4081 aaggagtggt tcatagctgc tttcctgatg caaaaacgag gctagtttac cgtatctgtg
     4141 gggggatggc ttgtagatat gatgacagga agagtttata gacacgcaaa aaagcccagt
     4201 cttccgactg ggctcttcgc ttaatttgat gcctggcagt tccctactct cgcatgggga
     4261 gaccc
//
LOCUS       DAWDRC010000350         4264 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8690_length_4264_cov_2.224994, whole genome shotgun sequence.
ACCESSION   DAWDRC010000350 DAWDRC010000000
VERSION     DAWDRC010000350.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8690_length_4264_cov_2.224994"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    232..241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    533..542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1385..1394
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1043 a          945 c         1105 g         1141 t
ORIGIN      
        1 gagcaaaaca tcacccgggc cgctcatgct ctgggaatgt cgcaacctgc ggtcagtaac
       61 gctgttgcac gcctgaaggt gatgtttaat gacgagcttt ttgttcgtta tggccgtggt
      121 attcaaccga ctgctcgcgc atttcaactt tttggctcag ttcgtcaggc attgcaatta
      181 gtacaaaatg aattaccagg ttcaggctct gagcctgcca gtagtgaacg tnnnnnnnnn
      241 nttgctcggc agtggattct gtttttatca gaacccgtat ctttatgttt tcctaatttt
      301 actcattttg ctttttctta ttttatatgc atgataaatc atattctcga ggattatttc
      361 tctgcattcc aataagggaa agggagttaa gtgtgacagt ggagttaagt atgccagagg
      421 tacaaacaga tcatccagag acggcggagt taagcaagcc acagctacgc atggtcgatc
      481 tcaacttatt aaccgttttc gatgccgtga tgcaggagca aaacatcacc cgnnnnnnnn
      541 nnatttcaac tttttggctc agttcgtcag gcattgcaat tagtacaaaa tgaattgcct
      601 ggttcaggtt ttgaacccgc gagcagtgaa cgtgtatttc atctttgtgt ttgcagcccg
      661 ttagacagca ttctgacctc gcagatttat aatcacattg agcagattgc gccaaatata
      721 catgttatgt tcaagtcttc attaaatcag aacactgaac atcagctgcg ttatcaggaa
      781 acggagtttg tgattagtta tgaagacttc catcgccctg aatttaccag cgtgccatta
      841 tttaaagatg aaattgcaaa aatcatccaa caattaaagg cccgttactg aaacatgatg
      901 tttataacga acaacatgct gcggtttcgc tcgatcgttt cgcatcattt agtcaaacct
      961 ggtatgacac ggtagataag caagccagta tcgcgtatca gggcatggca atgatgagcg
     1021 tacttagcgt ggtgtcgcaa acgcatctgg tcgctattgc gccgcgttgg ttggctgaag
     1081 agttcgctga atccttagag ttacaggtat taccgctgcc gttaaaacaa aacagcagaa
     1141 cctgttatct ctcctggcat gaagctgccg ggcgcgataa aggccatcag tggatggaag
     1201 agcaattagt ctcaatttgc aaacgctaac tgaatgcaga ataggtcgga catcaatgtc
     1261 tggtttattc tgcatttttt attgaatgta gaattttatt ctgaatgttt gtgctctcta
     1321 ttttaggatt aattaaaaaa atagagaaat tgctgtaagt tgtaggattc agccgattta
     1381 ttatnnnnnn nnnnaatttc atcctctgta atggaggatt ttatcgtttc ttttcacctt
     1441 tcctcctgtt tattcttatt accccgtgtt tatgtctctg tctgccaatt gcttaagcaa
     1501 gatcggacgg ttaatgtgtt ttacacattt tttccgtcaa acagtgaggc aggccatgga
     1561 gatgttgtct ggagccgaga tggtcgtccg atcgcttatc gatcagggcg ttaaacaagt
     1621 attcggttat cctggaggcg cggtccttga tatttacgat gcattgcata ccgtgggtgg
     1681 tatcgatcat gttttagttc gccatgagca ggcggcggtg catatggccg atggtctggc
     1741 gcgcgcgacc ggggaagtcg gcgtcgtgct ggtaacgtcg ggtccaggag cgaccaatgc
     1801 gattactggc attgccaccg cttatatgga ttccattcca ttagttgtcc tttccgggca
     1861 ggtagcgact tcgttgatag gttatgatgc ctttcaggag tgcgacatgg tggggatttc
     1921 tcgcccggtg gttaaacaca gttttctggt taagcaaacg gaagacattc cgcaggtgct
     1981 gaaaaaggct ttctggctag cggcaagtgg tcgtcctgga cccgtggtcg ttgatttacc
     2041 gaaagatatt ctaaatccgg cgaacaaatt accctatgtc tggccggagt cggtcagtat
     2101 gcgttcttac aatcccacga cctccgggca taaagggcaa attaagcgcg ctctgcaaac
     2161 gctggtggcg gcaaaaaaac cggttgtcta cgtaggcggt ggggcaatca cggcgggctg
     2221 ccatctgcag ttgaaagaag cggtggaggt gttgaatctg cccgttgttt cctctttgat
     2281 ggggctgggg gcgtttccgg caacgcatcg ccaggcactg ggcatgttgg gaatgcacgg
     2341 tacctacgaa gccaatatga cgatgcataa cgcggatgtg attttcgccg tcggggtacg
     2401 ttttgatgac cgaacgacga acaatctggc aaagtactgc ccaaatgcca ctgttctgca
     2461 tatcgatatt gatccaacct caatttctaa aaccgtgact gctgatatcc cgattgtggg
     2521 ggatgctcgc caggtcctag aacaaatgct tgaactcctg tcgcaagaat ccgcccatca
     2581 accgctggat gagatccgcg actggtggca gcagattgaa cagtggcgcg ctcgtcagtg
     2641 cctgaaatat gacactcaca gtgaaaagat taaaccgcag gcggtgatcg agactctttg
     2701 gcggttgact catggggacg cttacgtgac gtccgatgtc gggcagcacc agatgtttgc
     2761 tgcgctttat tatccattcg acaaaccgcg tcgctggatc aactctggcg gcctcggcac
     2821 gatgggtttt ggtttacctg cggcactggg cgtcaaaatg gcgttgccag aagaaaccgt
     2881 ggtttgcgtc actggtgacg gcagtattca gatgaacatc caggaactgt ctaccgcgtt
     2941 gcaatatgag ttgcccgtac tggtggtgaa tctcaataac cgctatctgg ggatggtgaa
     3001 gcagtggcag gacatgatct attccggccg tcattcacaa tcttatatgc agtccttacc
     3061 agacttcgtc cgtctggcgg aagcctatgg tcacgtcggg atccagatct ctcatccgca
     3121 agaactggaa agcaaactta gcgaggcgct ggaacaggtg cgcaataatc gcctggtgtt
     3181 tgttgatgtt accgtcgatg gcagtgagca cgtctacccg atgcagattc gcgggggcgg
     3241 aatggatgaa atgtggttaa gcaaaacgga gagaacctga ttatgcgccg gatattatca
     3301 gtcttactcg aaaatgaatc aggcgcgtta tcccgcgtga ttggcctttt ttcccagcgt
     3361 ggctacaaca ttgaaagcct gaccgttgcg ccaaccgacg atccgacatt atcgcgtatg
     3421 accatccaga ccgtgggcga tgaaaaagtg cttgagcaaa tcgaaaagca attacacaag
     3481 ctggtcgatg tcttgcgcgt gagcgagttg gggcagggcg cgcacgttga gcgggaaatc
     3541 atgctggtga aaattcaggc cagcggttac gggcgtgacg aagtgaaacg taatacggaa
     3601 atattccgtg ggcaaattat cgatgtcaca ccctcgcttt ataccgttca attagcaggc
     3661 accagcgata agcttgatgc atttttagca tcgattcgcg atgtggcgaa aattgtggaa
     3721 gttgctcgct ctggtgtggt cggactttcg cgcggcgata aaataatgcg ttgagaatga
     3781 tctcaatgcg caatttgcag cccaacatgt cacgttgggc tttttttgcg aaatcagtgg
     3841 gaacctggaa taaaagcagt tgccgcagtt aattttctgc gcttagatgt taatgaattt
     3901 aatccatacc agtacaatgg ctatggtttt tacattttac gcaaggggca attgtgaaac
     3961 tggatgaaat cgctcggctg gcgggagtgt cgcggaccac tgcaagctat gttattaacg
     4021 gcaaagcgaa gcaataccgt gtgagcgaca aaaccgttga aaaagtcatg gctgtggtgc
     4081 gtgagcacaa ttaccacccg aacgccgtgg cagctgggct tcgtgctgga cgcacacgtt
     4141 ctattggtct tgtgatcccc gatctggaga acaccagcta tacccgcatc gctaactatc
     4201 ttgaacgcca ggcgcggcaa cggggttatc aactgctgat tgcctgctcg gaagatcagc
     4261 ccga
//
LOCUS       DAWDRC010000351         4250 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8727_length_4250_cov_2.683671, whole genome shotgun sequence.
ACCESSION   DAWDRC010000351 DAWDRC010000000
VERSION     DAWDRC010000351.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8727_length_4250_cov_2.683671"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1674..1683
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1029 a         1138 c         1039 g         1034 t
ORIGIN      
        1 tccttagcaa gtaccacgct gctgactgct gattaattcg ccatcagcat tttggcttgc
       61 tgccctgagg cggcaagtaa cgtagcggat caattgccgt tgcacggtaa cgaatctgga
      121 aatgcaagcg aacagatgcc gcatcagtgc tccccatggt agcgattttt tgcccagcct
      181 tcacgctttg cccattattt accagcatcg tgtcattatg agcgtaagcc gtaatgtaat
      241 cttcactgtg tttaatcatg atgagattac cgtagccacg cagctggtta cccacataca
      301 ccacctttcc tgcacccgcg gcgtaaatag gtgtacctcg tggagctgag atatcaatcc
      361 ctttattgcc gccatctgct gtcgaatacg gcatgataac tttccctgtc gttggccata
      421 accaacaacg ttgccctact ggcggccagg aagatttcgg taccgctgat gacggtgtaa
      481 ccgatgcggt tttggtcgtt gatttggcgg ttgatttacg tgtactactg ctacttttcg
      541 ccccacccag ttttagtttc tgacctactt caatggtgta agggggggaa atgccgttca
      601 gtcgcgccag ttcttttacg ctggttcccg tggtgcgcga aatacgatat agcgtatccc
      661 cccgtttcac ggtgtaaacg gagccggaat acgttcctgt atcggatgat ttgctacccg
      721 aacagcccgc caaaagcagt ccaaccgata acaacatcac gatacccaga gattttttat
      781 tcaggcgtcc cgcactcaaa atctttcctc gcttaaaaaa caagacgcca tatcatagca
      841 gccagcctct tgatacccac tctcttgctt ctggaaaaac gatctaatag agaaagaggt
      901 taatttcatt tggcaaaggg aagtatcatc agcaaaatat ttgctgtagt ggtatcgaag
      961 ttcctgactt ttaccctatt tcccaccttg atgcgtaata atcctaattt atcacattga
     1021 ttttatcgcc actcatattg atacgtatca ctgtccctat tcagagtcct gtttcgtatc
     1081 atatctgtgt ttttttaacg tcatcacact tccttcctcc cctcacgcat atagtcgttt
     1141 ctggcgtaat tcttgcctgc taagactaaa tctttgccat aagaaccaaa tgttcaaacc
     1201 gaggggattt ctatggaagc gcgggaagca accgctacgg gtgaatcatg catgcgcgtc
     1261 gatgccattg ctaaggtcac cgggcgggca cgatatactg acgattatgt tatggcgggc
     1321 atgtgttacg cgaaatatgt acgtagccct atcgcacatg gttatgctgt aagtattaat
     1381 gatgaacaag ccaggagttt gccgggcgtc ctggcgattt ttacctggga agatgtgcca
     1441 gaaatcccat tcgccacggc agggcatgcc tggacacttg acgaaaacaa gcgcgatacc
     1501 gccgatcgcg cactgctaac tcgccatgtt cgtcatcatg gcgacgccgt tgccatcgtc
     1561 gtggcccgcg atgaactcac ggcagaaaaa gcggcgcaat tggtcagcat tgagtggcaa
     1621 gaattacccg ttatcacctc gccagaagcg gcgctggcag aagacgctgc accnnnnnnn
     1681 nnnacgccgc accaatccat aacggtggca atttactgaa acaaagcacg atgtcgacgg
     1741 gtaatgtcca acaaacaatc gatgccgccg actaccaggt acaggggcac tatcagactc
     1801 ccgttattca acattgtcat atggaaagcg tgacatcgct ggcatggatg gaggatgact
     1861 cgcgaattac catcgtttcc agcacccaga tcccgcacat tgttcgccgc gtggttggtc
     1921 aggcgctgga tattccctgg tcatgcgtac gagtcatcaa accgtttatc ggtggcggtt
     1981 ttggtaataa acaggatgta ctggaagagc caatggcggc attcctgacc agcaaacttg
     2041 gcggcattcc ggtgaaagtt tcccttagcc gtgaagagtg tttcctcgca acccgtaccc
     2101 gccacgcttt tactattgac gggcaaatgg gcgtgaaccg cgacggaaca ttgaaaggtt
     2161 atagtctgga tgttctgtct aacaccggcg cttatgcatc tcacgggcac tccattgctt
     2221 ctgctggggg gaataaagtc gcttaccttt atcctcgttg tgcctacgct tacagttcaa
     2281 agacctgcta taccaacctc ccctcggctg gtgcgatgcg tggttatggc gcgccacaag
     2341 tcgtatttgc cgttgagtct atgcttgatg atgccgcgac agcgttaggt attgatcctg
     2401 ttgaaattcg tttacgcaac gccgcccgcg aaggagatgc taatccgctc acgggaaaac
     2461 gtatttacag cgcagggttg ccggagtgtc ttgaaaaagg ccggaaaatc tttgaatggg
     2521 aaaaacgccg tgcagagtgc cagaaccagc aaggcaattt acgtcgtggc gttggcgtcg
     2581 cctgttttag ctacacctct aacacctggc ctgtcggcgt agaaatagca ggcgcgcgcc
     2641 tgttgatgaa tcaggatgga accatcaacg tacaaagcgg cgcgacggaa atcggccagg
     2701 gtgccgacac cgtgttctcg caaatggtgg cagaaaccgt gggagttccg gtcagcgatg
     2761 tccgcgtaat ttcgacacaa gacaccgacg ttacaccatt cgaccccggc gcatttgcct
     2821 cacgtcagag ctatgttgcc gcgcctgcgc tgcgcagtgc ggcactgtta ttaaaagaga
     2881 aaatcatcgc tcacgccgca gtcatgctac atcagtcagc gatgaatctg accctgataa
     2941 aaggccatat cgtgctggtt gaacgaccgg aagagccgtt aatgtcgtta aaagatttgg
     3001 cgatggacgc tttctaccac cctgaacgcg gcgggcagct ctctgccgaa agctccatca
     3061 aaaccaccac taacccaccg gcgtttggct gtacctttgt tgatctgacg gtcgatattg
     3121 cactgtgtaa agtcaccatc aaccgcatcc tcaacgttca tgattcgggc catattctta
     3181 atccgctgct ggcagaaggt caggtacacg gcggaatggg aatgggcatt ggctgggcgc
     3241 tatttgaaga gatgatcatc gatgcgaaaa gcggcgtggt ccgtaacccc aatctgctgg
     3301 attacaaaat gccgaccatg ccggatctgc cacaactgga aagcgcgttc gtcgaaatca
     3361 atgagccgca atccgcatac ggacataagt cactgggtga gccacccata attcctgtag
     3421 ccgctgctat tcgtaacgcg gtgaagatgg ctaccggtgt tgcaatcaat acactgccgc
     3481 taacgccaaa acgattatat gaagaattcc atctggcagg attgatttga ggataacatc
     3541 atgtttgatt ttgcttctta ccatcgcgca accacccttg ccgatgccat caccctgctg
     3601 gctgacaatc cgcaggccaa attgcttgcc ggtggcactg acgtactgat acagcttcac
     3661 catcacaatg accgctatcg ccatattgtt gatatccaca atctggcaga gctgcgggga
     3721 ataacacagg cggaagatgg cgcgctgcga atcggctccg cgacaacatt tactcagctc
     3781 attgaagatc ccgtaatcca acgcaatctc ccggcgttat gtgctgcggc tgcatcaatc
     3841 gccgggccgc agatccgtaa tgtcgccacc tacggcggaa atatttgcaa cggtgccacc
     3901 agcgcagatt ctgccacgcc aacgctaatt tatgacgcga tactggagct ccactgccca
     3961 cgcggtgttc gtttcgtccc gattaatggc tttcacaccg ggccgggcaa agtgtctctt
     4021 gagcatgacg aaatccttgt cgcctttcat tttccgccac agccgaaaga acacgcgggc
     4081 agcgcgcatt ttaaatatgc catgcgcgac gcaatggata tttcaacaat tggctgcgcc
     4141 gcacattgcc gactggataa cggcaatttc agcgaattac gcctggcatt tggtgttgcc
     4201 gcgccaacgc cgattcgctg ccaacatgcc gaacagactg cacaaaatgc
//
LOCUS       DAWDRC010000352         4250 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8726_length_4250_cov_3.155900, whole genome shotgun sequence.
ACCESSION   DAWDRC010000352 DAWDRC010000000
VERSION     DAWDRC010000352.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8726_length_4250_cov_3.155900"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          987 a         1088 c         1127 g         1048 t
ORIGIN      
        1 ggcgcaggcg ctgcatatgg gggatgaagt tcataaccgc aatgctgccg ctaccggttt
       61 gctgattaag cgcctgctcc cggcattgct ggcctgctct cttccacagg aacatatcca
      121 gcgcgtcgtg gcatttatca ccggtaacga tcacttcttc ctcaatctgt cgatggccgc
      181 ctgtaaagca atgatggatg ccgccgccaa tgtaccgttc agttcaatgg ttacggtgat
      241 ggcgcgtaac ggggtgaatt ttggtattcg gctttccggt actagcgatc gctggttcca
      301 ggctcctgcc aatgccgttg aaggtctgtt cttccccgga tttggcgttg acgacgctgc
      361 tgctgacctg ggtgatagcg caattactga aaccgcaggt atgggtggct tcgcgatggc
      421 gtcgtctccg gcgatcgtca aatttgtggg aggcacccct gccgatgcca ccaataacag
      481 ccgtcggatg caagccatca ccctcggcgg taaccccgca tttacacttc cagccctcaa
      541 ttttgccccc accgcagcag gaattgatgc gcgtaaagtg accgatcgcg gcattttacc
      601 ggtcatcaat acgggtatcg cccacaaaca agcgggggtc gggcaaattg gtgccgggat
      661 caccactgcg ccgatggcct gttttgtcgc cgctgtacgt gcactggcgg agatcgtcgc
      721 aaaggagaac catcatggtt aagccactgg ctgtcgtcgc ggttggcggc aatgcgctca
      781 ttcaggacga gcaacgcaat agtattcccg atcaatatgt tgcagtgatg gaaagcgtgc
      841 aacatatcgt tgatatggtt gaagccggat gggacctggt actaacccac ggtaatggcc
      901 cgcaggtggg ctttattctg cgccgctctg aactcgccag taacgaagtt tctccggttc
      961 cacttgatta cgccgtgggt gatacacaag gtgcaattgg ctacatgttc cagaaagcgc
     1021 tgcataacga attggctcgc cgtggcataa acaaaccggt aattgccctg gtgacacaaa
     1081 cgcgagtcag cccacatgac gatgctttcg ccagccccag caaaccaatt ggcgcgtttc
     1141 tcgatgaagc aacggcccaa caacgccaac aacaactcgg ctggacgctg atggaggacg
     1201 ccgggcgtgg ttggcggcgt acagttccct ctcctgcacc actggaaatt attgagcacg
     1261 acaccatcgc tcacctggtg cgccagggat atctggttat tgcctgcggc ggcggcggta
     1321 ttccggtggt gcgagacggg caacaactga aaggtgtaga agccgtgatc gataaagatc
     1381 tggcctccgc gctgctcgcc agtcagttag gcgcagatct gctggtgatc cccaccggtg
     1441 tagaaaaaat ggcgattaac tttggtacac cacaacaaca gtggctcgac gctatcagcg
     1501 ttgccgaagc gcaaacgctg ttgcgggaag gtcagtttgg tgtcggcagt atgcaaccca
     1561 aagtggaagc cattgttgat ttcatcaatg ccagccagca acaaggcaaa caggccagcg
     1621 gcctgattac ttcaccgcaa accataaaag cagccctggc gcatcagagc ggtacatgga
     1681 taacccttta attctctgga gaacacaatg actacaacgc ttttagccgt taacggaacg
     1741 ctgatgcgcg gcctggaact caacccaaat atgcaaaaag cgggcggtat ttttgtccgt
     1801 gaagaccgaa ctgacgccca ttaccggttg tggagtatta atgatcgcca tccgggaatg
     1861 attcgcgtga atgaaggagg aacacacgtc gacgtagaaa tttggcaatt accgctggcc
     1921 agttttgccg ctctgttaat gagcgaaccc gctgggctgg caattggcaa aatcaaactt
     1981 gccgatggta gcgaagtgct gggcgtactg gcagaaaact ggctgaccga aggccaacgg
     2041 gaaattacag aactgggcag ctggcgcaaa tacaccgggc attttcatac tgtgtaacgc
     2101 gcattcataa agggaacata ctatgcgtaa aaaagaagag aatctgaata cggcatcagg
     2161 attgcgtatt gccatgattt tgctgggtat tgccgtcaca cctgtgctgt tgtcatcctc
     2221 aagcctcggc aatcaacttt ccagcagcag tttaattagc gtcgtattgt taggcggcgt
     2281 cattctgacc ttactttcag ccatcaccat tagcgtggga gaaaaagccc gcctgccaac
     2341 gtatggcatt gtgaaatatt cgtttggcga aaaaggggcc atcgccatta acattttgat
     2401 ggcgataagt ctgttcggct ggattgccgt taccgccaat atgtttggtc attcggtaca
     2461 tgacttactg gctcaacatg gactggaagt tccactggca ctgttagtgg cggctggctg
     2521 tgtcattttt gtcgcctcta cggcatttgg ctttgccgtt ctgggaaaaa ttgcccaggt
     2581 tgccgtgccg gttatcgcgc tggtgctgtg ttacatcctc tatgtggcaa cccataccga
     2641 agtggcagta ccagcggcga ttgtggagat gaatacaggt gtcgccgttt ccaccgttgt
     2701 tggcaccatt attgtgctgg ttgccacact gcctgatttc ggtagttttg tgcataaccg
     2761 caaacatgcg ctgattgccg caggcgtgac gtttctggtt gcctaccctc tgctctactg
     2821 ggcgggtgca acgccgagcg ccattagtgg tcagggatct ttactgggtg cgatggcggt
     2881 attcggtgcg gttctgcctg cggcgctgtt gttgattttc gcctgcgtca ccggtaacgc
     2941 gggcaatatg ttccagggca cgctggtggt ttccacactg cttacccgct ttcccaaatg
     3001 gcagattacc gtggcgctgg gtatcctttc cgccatcgta ggcagtatgg atattatggc
     3061 gtggtttatt ccgtttctgt tgttcctggg tatcgccacg ccacccgttg ccggaattta
     3121 tatcgctgac tttttccttt atcgccgtaa tggctatcaa gagtcagtgt tagcccagga
     3181 gtcacagatt aaagtgctga cattcgcagc atggatcata ggcgcagcgg ttggctttat
     3241 gaccgtaaaa ggcttattca ccctgacgac gatcccttcg gtagactcga ttctggtggc
     3301 atgtatcgcg tatgcgattc tcagtcgggc aagtcaacac cgctaattcc tgggtatact
     3361 caggcaatga ttacggctga atctggagag attcagccac tttaccactg accagggagc
     3421 cttatgttca tctcagacga aacgcttcgt gttattcatc tggtcgccag acaccagagc
     3481 attaccaccg cagccgagca gttaaataaa gtcccttccg ccatcagtta caccatcaag
     3541 aaagcggaag agagcctggg cgttgaactg ttcattcgta agggacgcta tattgaatta
     3601 tcgccagcgg gaagttactt catcgaacat agcaaaacta ttcttggcga cctggaagca
     3661 ttaaagcgca acacagtgct gattcacgat ggtattgagc gtgaactgac aattgcggta
     3721 aacaatatta ttcctggtga tttgctggtg gcatttattc gagattttga acggcaattc
     3781 gcatcaacaa cactgacggt agatcttgag gtatacaacg gctgctggga tgcgctgtac
     3841 agtaagcatg ctgatctggt ctacggtgct cctcatgcag taccaagcag tgaaggcatc
     3901 attagcgaac cggtcggaca aatggaatgg gattttgtcg tcagcccact ccacccactg
     3961 gcagcgaagc ggcatccatt agaaaacagt gaattgcgtc attatccagc ggtttgtatc
     4021 cgcgatacct ccgttaactt ccccccgatg caagcctggt tgttggaagg acaaaaaccc
     4081 atttttgttc ctgacttcgc tacggcaatc gccttaatag aacaaaacgt cggtattggt
     4141 tatattccac atcatctggc attgccgtta ttgaacagcg gtaaattgct taaaaagcca
     4201 atgcgcgaac ataaacacgc caccaagtta tttcttgccg ctcgttccga
//
LOCUS       DAWDRC010000353         4244 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8742_length_4244_cov_2.238720, whole genome shotgun sequence.
ACCESSION   DAWDRC010000353 DAWDRC010000000
VERSION     DAWDRC010000353.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8742_length_4244_cov_2.238720"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2068..2077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3373..3382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          995 a         1016 c         1191 g         1022 t
ORIGIN      
        1 attattaacc tcgtggacgc gttaatggct aactcataat gggtattcaa taagctgcat
       61 tctgtgattg ttatcacatt tttgtttcgg gtgaatagag ggcatttttt cgttaatttt
      121 gattattaat cagtttgtta tgctctgttg tgagtaaaaa aacagcatct gactttcaat
      181 attggtgatc cataaaacaa tattgaaaat ttcttttttc tacgccatgt tttcaatatt
      241 ggtgaggagc ttaacaatat tgaaagttgg atttatctgc gtgtgacatt ttcaatattg
      301 gtgattaaag ttttatttcc aaattaaagg gcgtgatatc tgtaattaac accaccgata
      361 tgaacgacgt ttccttcatg atttctggag atgcaatgaa gattattact tgctataagt
      421 gcgtgcctga tgaacaggat attgcggtca ataatgctga tggttcgtta gacttcagca
      481 aagccgatgc caaaataagc caatacgatc tcaacgctat tgaagcggct tgccagctaa
      541 agcaacaggc agcagaggcg caggtgacgg ccttaagtgt gggcggtaaa gccctgacca
      601 acgccaaagg gcgtaaagat gtgctatcgc gcggtccgga tgaactgatt gtggtgattg
      661 atgaccagtt cgagcaggca ctgccgcaac aaacggcgag cgtactggct gcagccgctc
      721 agaaagcagg ctttgatttg atcctctgtg gcgatggttc ttccgacctt tatgcccagc
      781 aggttggttt gctggtaggc gaaatcctta atattccggc agttaacggc gtcagcaaaa
      841 ttatctccct gactgcagat accctcaccg ttgagcgcga actggaagat gaaacagaaa
      901 cgttaagcat tccgctgcct gcggttgttg ctgtttccac tgatatcaac tccccacaaa
      961 ttccttcgat gaaagccatt ctcggcgcgg cgaaaaagcc agtccaggta tggtcggcgg
     1021 cggatatcgg ttttaacgca gatgcagcct ggtcagaaca acaggttgcc gcgccgaaac
     1081 agcgcgagcg tcagcgcatc gtgattgaag gcgacggcga agaacagatc gccgcatttg
     1141 ctgaaaatct tcgcaaagtc atttaattac aggggatgct atgaacacgt tttctcaagt
     1201 ctgggtattc agcgataccc cttctcgtct gccggaactg atgaacggtg cgcaggcttt
     1261 agctaatcaa atcaacacgt tcgtcctcaa tgatgccgac ggcacacagg caatccagct
     1321 cggcgctaat catgtctgga aattaagcgg caaaccggac gagcggatga tcgaagatta
     1381 cgccagtgtc atggctgaca ctattcgcca gcacggcgca gacggtctgg tgctgttgcc
     1441 aaacacccgt cgcggcaaat tactggcggc gaaactgggt taccgcctta acgcggcggt
     1501 gtctaacgat gccagcgctg ttagcgtgca ggacggtaaa gcgacagtga aacacatggt
     1561 ttacggcggt ctggcgattg gcgaagaacg cattgccacg ccgtatgcgg tactgaccat
     1621 cagcagcggc acgttcgatg cggctcagcc agacgcgtca cgcactggcg aaacgcacac
     1681 cgtggagtgg caggctccgg ctgtggcgat tacccgcacg gcaacccagg cgcgtcagag
     1741 caacagcgtc gatctcgaca aagcccgcct ggtggtcagc gttggtcgcg gtattggcag
     1801 caaagagaac attgcgctgg cagaacagct ttgcaaggcg ataggtgcgg agttggcctg
     1861 ttctcgtccg gtggcggaaa acgaaaaatg gatggagcac gaacgctatg tcggtatctc
     1921 caacctgatg ctgaaacctg aactgtacct ggcggtgggg atctccgggc agatccagca
     1981 catggttggc gctaatgcgt cgcaaaccat tttcgccatc aacaaagata aaaatgcgcc
     2041 gatcttccag ttcgcggatt acggcatnnn nnnnnnngtc cgaagatatc tttgacgcca
     2101 tcatcgtcgg tgcagggctt gccggttcgg ttgccgcact agtgctcgcc cgcgaaggtg
     2161 cgcaagtgtt agttatcgag cgaggcaatt ccgcaggtgc caagaacgtc accggcgggc
     2221 gtctctatgc ccacagtctg gaacacatta ttcctggttt cgctgactcc gcccccgtag
     2281 aacgcctgat cacccatgaa aaactcgcgt ttatgacgga aaagtcagcg atgactatgg
     2341 actactgcaa tggcgacgaa acctcgccat cccagcgttc ttactccgtt ttgcgcagta
     2401 aatttgatgc ctggctgatg gagcaggctg aagaagcggg cgcgcagtta attaccggga
     2461 tccgcgtcga taacctcgta cagcgcgatg gcaaagtcgt cggtgtagaa gccgatggcg
     2521 atgtgattga agcgaaaacg gtgatccttg ctgatggggt gaattccatc cttgccgaaa
     2581 agctggggat ggcaaaacgc gttaaaccga cggatgtggc ggttggcgtg aaggaactga
     2641 tcgagttacc gaagtcggtt atcgaagacc gttttcagtt gcagggtaat cagggcgcgg
     2701 cttgcctgtt tgcgggatca cccaccgatg gcctgatggg cggtggcttc ctttatacca
     2761 atgaaaatac cctgtcgctg gggctggttt gtggtttgca tcatctgcat gacgcaaaaa
     2821 aatcggtgcc gcaaatgctg gaagatttca aacaacatcc ggccgttgca ccgctgatcg
     2881 cgggcggcaa gctggtggaa tattccgctc acgtagtgcc ggaagcaggt atcaacatgc
     2941 tgccggagtt ggttggtgac ggcgtattga ttgccggtga tgctgccgga atgtgtatga
     3001 acctcggttt taccattcgc ggtatggatc tggcgattgc cgccggggaa gcagcagcaa
     3061 aaactgtgct ttcagcgatg aaaagcgacg atttcagtaa gcaaaaactc gcggaatatc
     3121 gtcagcatct tgagagtggc ccgctgcgcg atatgcgcat gtaccagaaa ctaccggcgt
     3181 tccttgataa cccacgcatg tttagcggct acccggaact ggcggtgggc gtggcgcgtg
     3241 acctgttcac cattgacggc agtgcgccgg aactgatgcg caagaaaatc ctccgccacg
     3301 gcaagaaagt gggcttcatc aatctgatca aggatggcat gaaaggagtg accgttttat
     3361 gagtaaaatt tannnnnnnn nnttttatga cttctcccgt caatgtggac gtcaaactgg
     3421 gcgtcaataa attcaatgtc gatgaagagc atccgcacat cgttgtgaag gccgatgctg
     3481 ataaacaggc cctggagctg ctggtgaaag cgtgccccgc aggtctgtac aagaagcagg
     3541 atgacggcag tgtgcgcttc gattacgccg gatgtctgga gtgcggcacc tgtcgcattc
     3601 tggggctggg gtcggcgctg gaacagtggg aatacccgcg cggcaccttt ggtgtggagt
     3661 tccgttacgg ctgatgttgg tttgatacgt aatgccgtac tgactctcat tgcaaaaaaa
     3721 caggaataac ccatgcaacc gtccagaaac tttgatgatc ttaaattctc ctctattcac
     3781 cgccgcattt tgctgtgggg aagcggtggt ccgtttctgg atggttatgt actggtaatg
     3841 attggcgtgg cgctggagca actgactccg gcgctgaaac tggacgctga ctggattggc
     3901 ttgctgggcg cgggaacgct cgccgggctg ttcgttggca catcgctgtt tggctatatc
     3961 tccgataaag tcggacggcg caaaatgttc ctcattgata tcatcgccat cggcgtgata
     4021 tcggtggcga cgatgtttgt ctcatccccc gtcgaactgt tggtgatgcg ggtacttatc
     4081 ggcattgtta tcggtgcgga ttatcccatc gccacctcaa tgatcaccga gttctccagt
     4141 acccgtcagc gggcgttttc catcagcttt atcgccgcga tgtggtatgt cggcgcgacc
     4201 tgcgccgatt tggtcggcta ttggctttct gacgtggaag gcgg
//
LOCUS       DAWDRC010000354         4236 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8760_length_4236_cov_2.152117, whole genome shotgun sequence.
ACCESSION   DAWDRC010000354 DAWDRC010000000
VERSION     DAWDRC010000354.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8760_length_4236_cov_2.152117"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2713..2722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          859 a         1022 c         1221 g         1124 t
ORIGIN      
        1 ggaattgcca gccgggtcaa tgagatgctt gggctggtgc atatgcagga gttcgccaaa
       61 cgcaaaccgc atcatctttc cggtggtcag cgtcagcgtg tggccctggc cagaagcctt
      121 gctaagcgcc cgaaactatt actgctcgat gagccgatgg gcgcgctgga taaaaagctg
      181 cgtgaccgga tgcagcttga agtggtggat attctggagc gcgtcggtgt gacctgcgta
      241 atggttactc acgatcagga agaggcgatg accatggcgg ggcgcatcgc cattatgaat
      301 cgtgggaaat ttgtccagat tggcgagccg gaagagatct acgagcatcc gaccactcgc
      361 tacagcgctg aatttatcgg ttcggttaat gtctttgaag gtgtactcaa agagcgtcag
      421 gaagatggtc tggtgcttta ttcgccgggg ctggtgcatc cactgaaagt cgatgcggat
      481 gcttcagtgg tcgataacgt gccggtgcat gtggcgcttc gcccggaaaa aatcatgctt
      541 tgcgaggaac cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc
      601 tatctcggcg atctttcggt gtatcacgtt cgcctgaaaa gtgggcagat gattagcgcc
      661 cagctacaaa acgcccatcg ccatcgtaaa gggttaccga cctggggcga cgaagtgcgt
      721 ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgagtaca
      781 cttgaacctg ctgcccagcc aaaacccttt aagctgtggt tgtcgcagct gcaaatgaag
      841 catgggcgca aactggtcat tgcgttgcca tatatctggt tgatcttgct gtttctgctg
      901 ccatttctga tcgtctttaa aataagcctt gcggagatgg cgcgcgctat tccaccttat
      961 accgaattga tggagtgggc tgacgggcaa ctttctatca ctcttaatct cggtaatttt
     1021 ctgcaactga ccgacgatcc gctctatttt gatgcttatc tccagtcgtt acaggtggcg
     1081 gcgatttcga ctatttgctg tttactgatc ggctatccgc tggcgtgggc ggtggcgcac
     1141 agtaagcctt cgacccgtaa tatcttatta ctactggtga tcctgccgtc gtggacctcg
     1201 tttctgattc gtgtctacgc ctggatggga atattaaaaa acaacggtgt gctgaataat
     1261 tttctgctgt ggctggggat tatcgatcaa ccgctgacca ttctgcatac caactttgct
     1321 gtttatattg gcattgttta cgcctacgtg ccgtttatgg tgctgccgat ttacaccgcg
     1381 ttgattcgta ttgattattc gctggtagaa gcggcactgg atctcggtgc acgaccgctg
     1441 aaaacgttct ttactgtgat cgtgccgcag actaaaggtg ggattattgc cggatcgatg
     1501 ctggtgttta tcccggctgt gggcgagttt gtgatcccgg aactgctcgg tggcccggac
     1561 agcatcatga tcgggcgcgt gctgtggcag gagttcttta acaaccgcga ctggccggtg
     1621 gcctcagcgg tagcgatcat catgttgctg ctgctaatta tgccgataat gtggttccac
     1681 aaacatcagc aaaaaagcgt gggagaacac ggatgaataa tttaccggtg gttcgttcgc
     1741 cctggcggat tgtgattttg ctgttgggct tcacctttct ctacgcgcca atgctgatgc
     1801 ttgttatcta ttcgtttaac agctcgaagt tagtgacggt gtgggccggc tggtcaacgc
     1861 gctggtatgg cgaattattg cgcgatgacg caatgatgag tgcggttggt ttaagtctga
     1921 caattgcggc ctgtgcggcg acggcggcgg cgatcctcgg gactattgcg gcggtcgtgc
     1981 tggtgcgctt tggcaggttt cgcggatcaa atggttttgc cttcatgatc accgcaccgc
     2041 tggtaatgcc agatgtcatc acgggtttgt cgctgttgtt gttattcgtc gcgcttgctc
     2101 atgctattgg ctggcctgcg gaccgcggta tgctcaccat ctggctggcg catgtcactt
     2161 tctgtacggc ttatgtggga gtcgttattt cgtcgcgtct gcgggaactg gatcgctcga
     2221 tagaagaagc cgcgatggat ctcggtgcga cgccgctgaa agtgtttttt gtcattacgc
     2281 taccgatgat catgcctgcg atcatttctg gctggttact ggcttttact ttgtcgcttg
     2341 atgatctggt gatcgccagc tttgtttccg ggccgggagc caccacttta ccgatgctgg
     2401 tcttttccag cgtgcggatg ggggtgaatc cggaaatcaa cgccctggca acattgatcc
     2461 tcggcgcggt cggaattgtc ggatttatcg cctggtatct gatggcgcgc gcagaaaaac
     2521 agcggatacg cgacatccag cgtgcaagac gtggctgaag acactaaaat ttgccaacct
     2581 ggctacataa tgccgcgcat gtcgcggcat tgttttcatg gaagacgaaa cgttgggatt
     2641 ttttaagaaa acatcttcat ctcatgctcg cctgaatgtg cctgcgctgg tgcaggtggc
     2701 ggcgctcgcc atnnnnnnnn nnctgttgat tttcaatacg ctgggcgtgc gcggtattgg
     2761 cgagttcatt catcgcagcg tacaaacctg gagtttaacg ctggtctttt tgagcagtct
     2821 ggtgctggtt ttcattgaga tctggtgtgc gttttcactg gtgaaagggc gtcgctgggc
     2881 gcgctggctg tatctgttga cacaaatcac agccgcaagt tacttgtggg cggcttcgct
     2941 ggggtatggt tatccggaac tgttcagcat tcccggtgaa tcaaaacgtg aaatcttcca
     3001 tagcctgatg ctgcagaagc tgccggatat gctcatcctg atgctgctgt tcgttccctc
     3061 gactagtcgg cggttcttcc agttgcaata atgtgtataa tcgtcgcccc tgatgatgtg
     3121 aaggtcaatg tatgcagtgc gcactttacg acgcgggtcg ctgtcgttcc tgtcagtgga
     3181 taacgcagcc gattccagag caactctccg ctaaaaccgt cgatcttaaa aatctgctcg
     3241 ccgattttcc ggttgaggaa tggtgtgcgc cggtgtcagg cccggaacaa gggtttcgta
     3301 ataaagccaa aatggtggtg agtggtagcg ttgaaaaact actgctcggt atgctgcatc
     3361 gggatggtac accggaagac ctttgtgact gcccgcttta tccagcctca tttgcgcccg
     3421 tttttgcggc gctaaaaccg tttatcgccc gtgcgggttt aacgccttac aacgtggcgc
     3481 gtaaacgtgg tgaactgaaa tacattctgc tgactgaaag ccagagcgat ggcggcatga
     3541 tgctgcgttt tgtactgcgt tctgatacca aactggcgca actgcgtaag gcgctgccgt
     3601 ggttacagga acaattaccg cagctgaaag ttattaccgt caatattcag ccggtacata
     3661 tggcgattat ggaaggggag acggagatct acctgaccga acaacaggcg ctggcagagc
     3721 gttttaatga cgtaccgctg tggatccgtc cgcaaagttt cttccagact aatccggcgg
     3781 tcgccagcca gctgtacgcc accgcgcgcg actgggtacg acagctgccg gttaaacata
     3841 tgtgggatct gttctgcggt gtgggtggct ttggtttaca ctgcgcgacg cctgacatgc
     3901 agttaaccgg gatcgaaatt gcaccagagg ccattgcctg tgcgaagcag tcagccgctg
     3961 aactgggctt aacgcgtttg caatttcagg cgctggactc tacgcagttt gccaccgctc
     4021 agggggaagt gccggagctg gtgctggtta acccgccgcg ccgcggcatc ggtaaaccgc
     4081 tgtgtgatta tctctcaacg atggcaccgc gttttattat ctactccagc tgtaacgccc
     4141 aaactatggc gaaagatatc cgcgaactgc ctggttatcg catcgaacgg gtacagcttt
     4201 ttgatatgtt cccgcacacc gcgcactatg aagtgc
//
LOCUS       DAWDRC010000355         4226 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8782_length_4226_cov_2.678494, whole genome shotgun sequence.
ACCESSION   DAWDRC010000355 DAWDRC010000000
VERSION     DAWDRC010000355.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8782_length_4226_cov_2.678494"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2664..2673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1211 a          884 c          943 g         1178 t
ORIGIN      
        1 attattcttt gatatttatg gatattacgc atctgatatt tttgtagcca aaaataatga
       61 taaggtaatg cttttcattc ctggtgcaaa aaaacctttt ttattcaaga agaatatcgc
      121 tgatttgcgg cttaccctta aagaacttat taaggatagt gacaacaaac aattactttc
      181 ccaacatttt tcattatata gtcgtcaaga tggagtttcc tatgcaggag taaattctgt
      241 tctacatgca atagaaaatg atggtaattt taatgagtct tactttctgt attccaataa
      301 gacacttagc aataaagatg tttttgatgc tatagctatt tctgttaaga aacgcagttt
      361 cagtgatggt gatatcgtta taaaatcaaa cagtgaagct caacgagact atgctctgac
      421 tatactccag acgattttat caatgacccc tatatttgat atcgtagtcc cggaggtatc
      481 tgttccgctt ggactgggga ttattacttc cagtatgggg atcagttttg atcaactgat
      541 taatggtgat acttatgaag aacgtcgttc tgctatacct ggtttggcga caaatgcagt
      601 attgcttggt ctgtcttttg caattccact cttgattagt aaggcaggaa taaaccagga
      661 ggtacttagc agcgttataa ataatgaggg caggactctg aatgaaacaa atatcgatat
      721 atttttgaag gaatatggaa ttgctgaaga tagtatatcc tcaactaatt tgttagacgt
      781 taagcttaaa agttccgggc agcatgtcaa tattgtaaag cttagtgatg aagataatca
      841 aattgtcgct gtaaaaggga gttctctgag cggcatctac tatgaagtgg acattgaaac
      901 aggatatgag attttatccc gaagaattta tcgtaccgaa tataataatg aaattctctg
      961 gactcgaggt ggtggtctaa aaggggggca gccatttgat tttgaaagtc tcaatattcc
     1021 tgtatttttt aaagatgaac cctattctgc agtgaccgga tctccgttat catttattaa
     1081 tgatgacagc tcacttttat atcctgatac aaacccaaaa ttaccgcaac caacgtcaga
     1141 aatggatatt gttaattatg ttaagggttc tggaagcttt ggggatagat ttgtaacttt
     1201 gatgagagga gctactgagg aagaagcatg gaatattgcc tcttatcata cggctggggg
     1261 aagtacagaa gaattacacg aaattttgtt aggtcagggc ccacagtcaa gcttaggttt
     1321 tactgaatat acctcaaatg ttaacagtgc agatgcagca agcagacgac actttctggt
     1381 agttataaaa gtgcacgtaa aatatatcac caataataat gtttcatatg ttaatcattg
     1441 ggcaattcct gatgaagccc cggttgaagt actggctgtg gttgacagga gatttaattt
     1501 tcctgagcca tcaacgcctc ctgatatatc aaccatacgt aaattgttat ctctacgata
     1561 ttttaaagaa agtatcgaaa gcacctccaa atctaacttt cagaaattaa gtcgcggtaa
     1621 tattgatgtg cttaaaggac ggggaagtat ttcatcgaca cgtcagcgtg caatctatcc
     1681 gtattttgaa gccgctaatg ctgatgagca acaacctctc tttttctaca tcaaaaaaga
     1741 tcgctttgat aaccatggct atgatcagta tttctatgat aatacagtgg ggctaaatgg
     1801 tattccaaca ttgaacacct atactgggga aattccatca gactcatctt cactcggctc
     1861 aacttattgg aagaagtata atcttactaa tgaaacaagc ataattcgtg tgtcaaattc
     1921 tgctcgtggg gcgaatggta ttaaaatagc acttgaggaa gtccaggagg gtaaaccagt
     1981 aatcattaca agcggaaatc taagtggttg tacgacaatt gttgcccgaa aagaaggata
     2041 tatttataag gtacatactg gtacaacaaa atctttggct ggatttacca gtactaccgg
     2101 ggtgaaaaaa gcagttgaag tacttgagct acttacaaaa gaaccaatac ctcgcgtgga
     2161 gggaataatg agcaatgatt tcttagtcga ttatctgtcg gaaaattttg aagattcatt
     2221 aataacttac tcatcatctg aaaaaaaacc agatagtcaa atcactatta ttcgtgataa
     2281 tgtttctgtt ttcccttact tccttgataa tatacctgaa catggctttg gtacatcggc
     2341 gactgtactg gtgagagtgg acggcaatgt tgtcgtaagg tctctgtctg agagttattc
     2401 tctgaatgca gatgtctccg aaatatcggt attgaaggta ttttcaaaaa aattttgatt
     2461 cgggaaatta tttattggcc attgttatag ttactccctt gtgctgtttt gttagcgcaa
     2521 gagagtgtca gttctgcatt ttgatatatt caagtcacca tcctccaaag gcaaacaaat
     2581 gagaaaaggt catcaggcac aaacatccag tgaaaagctc cccagtcttc tcctgctggc
     2641 ttctgccccc tactttgcca caannnnnnn nnncccctgt cggtcagtat tctctacatt
     2701 ggcaggtcat gcgactcata aaatggcagt acacgatcat tcagcaggcc agtcgctgta
     2761 atcgcatttt ggtgacatac aatttgcagt gaacgacctt tgagtatgta tcaacaaagg
     2821 tctggtgata aattacgcga cacctttcag gttgcctaca tagaacgtgt cctgcgaacc
     2881 cagatatccc ggatgagccg tttcgatttc gccacaggcc tcgtcatcac tggctttacg
     2941 ctccagtgct gcgatctggc tgtcagttag ctcaatgcca tcacgggcta ctttttcttc
     3001 cagtgctttc agacgttttt tgaaattctc taggttattg cgtaaccaga cagaacggac
     3061 accactgccg gagataaaaa cgccctgttt acgcagcttg ttgctggtcc ggtgctgacc
     3121 gtgtgccggg aaagcgacgg cgtaatcaac aacagcctgt tcagttgcct catcggtacg
     3181 gttcttaagg ttaggagcgc ggcgactacg attaatcagc gcatccacac cgccttcatc
     3241 gaccagttcg cggtaacgat aaaatgtatc acgcgaaacg cccatgattt tacagacttt
     3301 tgatacgttg ctgagtgatc ttacccacca atagtggaca caggactaag tgagtaaact
     3361 ctcaaccaga ggtgactcat gacaaaacca gtatcaatca gcaagaagcc ccgtaaacaa
     3421 catacgcctg aatttcgtaa cgaagccctg aaactcgctg aacgcatcgg tgtggccgcc
     3481 gcagcccgtg aactcagcct gtatgaatct cagctttatg cctggcgcag taaacagcag
     3541 caacaaatga gttcgtcaga gcgcgaaagc gaactggccg ctgaaaatgt ccgccttaaa
     3601 cgacaactgg cggagcaggc tgaggaactg gccatcctcc aaaaggccgc gacatacttc
     3661 gcgaagcgcc tgaaatgaag tatgtcttca tcgaaaatca tcgggcagag ttcagcatca
     3721 aagcgatgtg tcgtgtactt cgggttgccc gcagcggctg gtatgtctgg ctcaggcgtc
     3781 gtcaccagat gagcctgcgc caacagtttc ggctcacctg cgatgccgct gtccataagg
     3841 cattcattga ggcaaagcag cgatacggtg ctccccgcct tgctgacgaa atgccggagt
     3901 tcaatattaa aaccattgcc gccagcctgc gtcgtcaggg gctgcgggcg aaagccagcc
     3961 ggaagttcag cccggtcagc taccgtgcac atggcctgcc cgtattggag aatctgctgg
     4021 agcaggactt cagcgccagc ggcccgaacc agaagtgggc gggtgacatc acgtacttgc
     4081 gtaccgatga gggctggttg tatctcgcag tagtcatcga cctgtggtca cgcgccgtta
     4141 ttggctggtc gatgtcaccg cgaatgacag cacaactggc ctgtgatgca ctgcaaatgg
     4201 cgttgtggcg gagaagacgc ccggaa
//
LOCUS       DAWDRC010000356         4209 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8826_length_4209_cov_1.898893, whole genome shotgun sequence.
ACCESSION   DAWDRC010000356 DAWDRC010000000
VERSION     DAWDRC010000356.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4209)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8826_length_4209_cov_1.898893"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    743..752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1207..1216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1982..1991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3373..3382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1146 a          941 c          935 g         1147 t
ORIGIN      
        1 aataatttat tttatttcaa taagatagtt caatacacct gagaatacca tgctctcatt
       61 tcctaatgag ttgttgcatt ttttcatatg ttacaatata acattacaca tcatgtacat
      121 taactctgga ggaaattgtt ttggcgattc gtcttcataa actggctgtt gctttaggtg
      181 tctttattgt tagcgctcct gccttttcgc atggccatca ctcacacggc aaacccttaa
      241 cagaggtcga acaaaaagct gctaatggtg tttttgatga taccaatgta caaaaccgaa
      301 cgctcagtga ctgggatgga gtctggcaat ccgtttaccc tttactgcaa agtggcaaac
      361 ttgaccccgt ctttcagaag aaagcggatg cagataaaac caaaacattt gctgaaatta
      421 aagattatta tcgcaaaggt tatgcgacag atatcgaaat gattggcatt gaggacggca
      481 ttgtcgaatt ccatagaaat aatgaaacaa catcctgtaa atatgattac gatggataca
      541 aaatactcac ctataaatca ggcaagaaag gcgttcgtta cttattcgaa tgtaaagatc
      601 ctgaaagcaa agcccctaaa tatatacaat ttagcgatca tataattgca ccacgaaaat
      661 catctcattt tcacattttt atgggtaatg attcccaaca gtctttattg aatgaaatgg
      721 aaaactggcc aacgtattat ccnnnnnnnn nnttatccct atcagttgag tagcgaagaa
      781 gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta tttaacagaa
      841 cagtgcctgg tagtaacaac aataatttgc caagacaaat aagtaagaca aactcagaca
      901 cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg cgaaaaaggc
      961 gaacaataaa tacacaactc agccatacag actggcagcg caaatgtttc caggagcaag
     1021 tcatctgtca tggtaaagct aaccagaaat ataggttaat tttggtgttt aaaccttttt
     1081 tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa
     1141 attatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt ggctcaatta
     1201 ttgaccnnnn nnnnnnccga taagaaaacg actgaataac tgtagatttt cgatcgaaac
     1261 cttcctgtca gctccatagc gaatcaagtg ctgaatgtca cagtatcgaa cagaaaacag
     1321 tgacgatcta acccttcaag aatattctac gattgttctg tttaggaaaa gcaaggcggg
     1381 aagtcgggag ataagtcatt gataaagtgg cggagagagg gggatttgaa cccccggtgg
     1441 agttgccccc actccggttt tcgagaccgg tccgttcagc cgctccggca tctctccgtt
     1501 cagatggttg ccatgatgcc aggaaatttg gcattttaac agtccctgtc cgtgcaattt
     1561 tgttcaagtg acgagtttgc gagcaaaacg atgattaagt ggccctggaa agtacaagaa
     1621 tcagcacatc aaactgccct tccctggcag gaagcactat cgatccccct tttaacgtgt
     1681 ctgacagaac aggaacaaag caaattggtc gctcttgccg aacgtttttt acagcaaaaa
     1741 cggcttgttc ctttacaggg ctttgagctg aattcattaa gaagctgccg gatagcactt
     1801 ctattttgcc tgcccgttct ggagttagga ctggaatggc tggatggttt tcatgaagtc
     1861 ttaatttatc ctgcgccatt tgtggtcgat gatgaatggg aagacgatat cggtctggtg
     1921 cataaccaac gtattgttca gtcaggtcag agctggcagc aagggcctat cgttttgaac
     1981 tnnnnnnnnn ncggagttcc ctttatttcg ttgcgtgagg ttgctggctg ggaacacgat
     2041 cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tagttggtga gaatgcggcg
     2101 agcattgatg cttatgctgc cagtgatcct gctgaatgtt ttgccgtact ttctgaatat
     2161 ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca acgtttctgt
     2221 caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac agacccgttt
     2281 tcggcgacga atgttcatta attaacaact ttgcagatta attaaccaat tgaaatggct
     2341 tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc gttctcacga
     2401 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     2461 tccagtcaga ggagccaatt tcctgctttc atgcatcctt gcgaatcctt atgttatttt
     2521 tattcaacag gttagcgtga aaactcttcc cggtgcattt tgattttacc ctctgcatcg
     2581 ggaaaaattg gtggtcaaat ctggggtcag gttagttcga taatggagtg acccccatat
     2641 gtcccttaac gacgcaaaaa tccgtagtct caagcccact gataaaccct ttaaagtctc
     2701 cgattcccac ggtctgtatc tgctggtcaa accaggcggt tcccgtctct ggtatcttaa
     2761 ataccgtatt aatggcaaag aatcccgtat tgcactgggt gcctaccctg ccgtctccct
     2821 gtctgacgcc cgccagcagc gcgaaggcat ccgtaagatg ctggcgctga acatcaaccc
     2881 ggcgcagcaa cgtgccgctg aacgcggctc gcgcatgcag gagaaaatgt ttaaatccgt
     2941 ggcactggag tggcacagta gcaagaaaaa atggtcgcag aataccgcag atcgcgtgct
     3001 tgcccgtctg aacagacatg tctttccgac catcgggcat ctaccagtta ccgagcttaa
     3061 atcacgtcat ttcattgaat tactgaaagg cattgaggaa aagggcttgc ttgaagttgc
     3121 gtcccgctcg cggcaacacc tcagcaacat tatgcgttat gctgttcatc aggggttaat
     3181 cgaaatcaac ccagctgcaa atcttgacgg cgtgacggct tctcccgcca gacgacatta
     3241 tcccaccctg ccactggagc gccttcctga gctgcttgaa cggattgaca gttatcacca
     3301 aggacgggaa ctcaccaggc ttgcagtcct gctgacgttg catgtgttca ttcgctcaag
     3361 tgaactgcgc tannnnnnnn nngcgctatg ctcgttggac agagattaac ttcagaaatc
     3421 ggatctggac gatacctgcc acacgcgaag cgattgcagg cgtacgttat tccagtcgag
     3481 gtgccaaaat gcgtacaccg catattgtgc ccctttctga gcaggttatc tctattctga
     3541 aaaggattaa ggaaatatct ggcgggtatg aactggtgtt ccccggctat catgacccct
     3601 acaagccgat gagtgaaaat actatcaata aggcacttcg ccagatggga tacaacacga
     3661 aacaagatat ctgcggtcat ggtttccggg caatggcgtg tagcgcgctg atggaatccg
     3721 gtttatggtc gcaagatgca gtggagcgtc agatgagtca ccaagagcgc aacactgtgc
     3781 gtctggctta cattcataag gccgaacata tggaagcccg catggacatg atgcagtggt
     3841 ggtcggatta tctggatatg tgcagtgaaa tctgggtgcc cccgtacatc tggagtcagc
     3901 aaaacattaa tctcgcagta acctgactta tgaataatct tgtaaagaac caggccgtat
     3961 cagcaaacgc tgatacggct tttcatccac aggccgtttt tcatcttcta agtcgtccag
     4021 gacatcatgg ttcttaatac acatgcgttc gcatgtgctt atgttaagta gacttattgt
     4081 cttacacatt ttatccgtat cattaaccag aatatgttcc ggagcagcgg gtgatagtgc
     4141 cgccagtcca cactcatcgc tgcccggaaa aatcatcagt ggggaggcaa acggtaagca
     4201 ccccgctat
//
LOCUS       DAWDRC010000357         4184 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8878_length_4184_cov_1.961976, whole genome shotgun sequence.
ACCESSION   DAWDRC010000357 DAWDRC010000000
VERSION     DAWDRC010000357.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8878_length_4184_cov_1.961976"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    644..653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1835..1844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1145 a         1043 c         1036 g          940 t
ORIGIN      
        1 cccgtgctgc aataattgca ctgttatccg ggcgttcgcc cgctttaatc acaataggct
       61 gtgtagcctg ggcctgtttc tctttcaccc gcgccagggc ggcagcaatc gcatctttat
      121 ctttggctgc aggttgaacg gctgcgctct tatgtcgttc aaggcgagcc gctttttcgc
      181 gctccagacg agcctggcgc gcttcgaaac gcgctttggc ttctgcggcg cgcttttctt
      241 cctgacgaat agccgcaatt tcagcttttt cctgacggaa atattgcact agggggatat
      301 tgctcgggca aacccacgcg caagctccac attcaatgca gtcggcaatg ttatgcgtgg
      361 tggctttatc gtgttgctga cctttgctga accagtacag ctgttgcggc aaaagatccg
      421 cagggcaggc gtcagcacag gcgctacaac ggatgcaact ttgttcttcc tgtggttcgc
      481 caagttcatt ggcagaggga gccaacagac agttggtaat ctttacgacc gggacatcca
      541 gccacggcaa ggtaaagccc attagcgggc cgcccataat caccatttgg tcggcagagg
      601 ggcagaatcc ggcgtcattc aaaaaatgac gcactggcgt cccnnnnnnn nnnagcgatt
      661 gcttcgccag tcagggtcac gacacgctcg gtaatcggct cgccatcaat aacggcacgt
      721 ttcactgcat aagcggtgcc gacgttttgc attaatacgc cgatatcgga tgaacgcccg
      781 ccatgtggaa cctgcttccc ggtcagaatg taggttaatt gtttagcacc gccagaagga
      841 tatttggtgg gaatcacccg cagagaaata tcgtgagagt ctgccagcac cgcgcgcagc
      901 atggaaatcg cctgcggttt gttatcttca ataccgataa gaatttcgcg tggctgcaga
      961 atatgcgcaa ggatgcggat accctctacg acctgagccg cacaatcctg catcaaacgg
     1021 tcatcggcgg taatgtacgg ctcgcactca gcagcgttga taatcaacgt ttcaatctta
     1081 tctccgccgc cctgcaattt aacgcctgtc ggaaatcccg caccacccag cccggcaaca
     1141 ccaaactgat gaatgcgctc gattaactct tcgcgactgc gagagcgata atcggcccag
     1201 ccgtcgcgcg ggatccagca gtcttcgcca tcggcatcaa taatcacact taattcagct
     1261 aaagctgaag gatgagccgt agagtggggc gcaatagccg taacggtacc cgaggtgggc
     1321 gcgtgaacag gcagcatttt gccgcgacca cgggtaagcg gctggccgcg caatacgtta
     1381 tcgccgacgc taacgcacaa ctcaccttca gcgccaatat gttgtttcag tggaatgaca
     1441 aaacgctgcg ccagtggtac ctggcgcagg ggcgtaccgt tggactgggt tttcatctcc
     1501 ggtggatgga tgccgccgtt gaaatcccag attttatttt ttctgaatgc agagaatgac
     1561 ttaagcatgg tgttccacgg gaatgatacg cacgggaatg gtgttcagat cccatttcca
     1621 ggagtcagga gtttctgcga ccggttgcaa cgagatgcag tgtgtcgggc acggatcaac
     1681 acataaattg cagcccgtac agagatcact cattaccgta tgcatggcgc gggtagcgcc
     1741 aacgatggcg tctaccggac acgcctgaat acatttagtg cagccaatac agttattttc
     1801 atcaataact gccaccatcc gcgctggcgt tagcnnnnnn nnnnccaccg caaaacggcg
     1861 ggaggcataa cccagaatgg cgccaaacgc caggcccagc aggctcacgg cggcaacggc
     1921 aatccagata gcattcatta caacttcacc aaaccactaa agcccataaa ggccagagac
     1981 ataagacctg cggtaattaa cgcaatggca ttaccgcgaa aaggtgccgg gacatcagcc
     2041 acagcaaggc gttcgcggat ggcggcgaag agcaccatca ccagcgagaa accgacagcg
     2101 gcggaaaaac cgtacagcgc tgactgcaag aaattgtgac cgagattgat attcagcaac
     2161 gccacgccga gcacggcaca gttggtggtg ataagcggca aaaaaatccc cagcagacgg
     2221 taaagtaccg ggctggtttt gcgcaccacc atttcggtga actgcacaac cacagcaatc
     2281 accagaataa acgccagggt gcgcaggtaa atcagattaa gtgggatcaa aatccacgta
     2341 tcgataagcc aggcgcaaat agacgccagc gtcattacaa acgttgttgc cagccccatg
     2401 cccatcgcgg tttccagctt tttggaaacc cccataaacg gacagagacc gagaaacttg
     2461 accagtacaa agttattgac cagtacagtt ccgacaaaca gtaacaggta gtcagtcatt
     2521 tttcggcctg aaataaaaaa gccgcctatt atccgttaat ccagagcagg cgacaacagg
     2581 ttaactgtaa ggttattacg gattcacaaa cgtcgctttc acccgcgacg agcgtttgaa
     2641 ataggggacg atcaatgcgg ttgccaacaa agtaaacagc aactggcgaa cggctatgcc
     2701 atcttctacg ggtgaaaaag cgaacgcttt tactgccagc aatacggaaa taagcagcca
     2761 gataatatag tgtttaggaa cacaacgccg acgtttaaag aaagcgatgg tcaaccacag
     2821 tgtgtaatac cacatggcga tagcggtaat aaaagaaacg ccccataaga tttgtgtcgt
     2881 cagtgcttgt ccgccgagcg tttgaaatgt ttgcggagaa gataacgcag cggtatacag
     2941 caacaacgcc agcgtcgtac tcaacagagc gactaaaagc caggccaacg ggccaagcaa
     3001 ccagcctcca atacgttgtg gcgttgtggt ggtcataggt tctccagaat agcgacgcaa
     3061 acagacaaat ctaattttag gggcgttatt ataaggcttt tataaccgat cgctactttc
     3121 tttactggac atagcgccag acggacttcg gtacctggcc gaggtcaaac aaacgcccgc
     3181 cagaaaccag ctctgcgcga cggtgatcgg cagcacgata catattgatc agttcctgat
     3241 cgtccgtcag ggtgtaatta agatggtcgt agagtttttc cagactttcg agtgaactga
     3301 ttttgcgaaa ttttaataag tagtcctgaa cagtcataaa aggtccataa aataattaat
     3361 accaaagggg taggtattat tagtgatgat aaaatttcac caaaagtgaa cgcttgtgtt
     3421 attacaataa taagaatgtg ctgatggcaa atattcaggg atgaaaaact gctctggaaa
     3481 gagtaacgcg ctttactatc cctgacaatc atcaacaaca tcgaacaaga taataaattc
     3541 ctggtttaat atccgacaag tgaaaacatg cacccggacg ggcagcatgt cgctcaacaa
     3601 gtgcagagct tacttgtgtt gtactgtagt actctgttca ggtggctgat agttgtcaat
     3661 gtgactcgcc acgccaagaa gaatgactga aacgacaaga acgatccaac ctgttaattc
     3721 aataagacga ttcattacag cccacatctc tgttgattga tccattaact tcagggggta
     3781 aatgttactt agcaataata gctccagcaa gatttttact gaggtttttg caatatttag
     3841 cttttgtcgt tggaaaaatc gctatttttt tgacttaagt taaacaacat cccttattgc
     3901 tggcaggtta ttaaactgtt gagcgagggt aaaggatagt gtcaaatagc catcatactt
     3961 caatgagagg caatgacatg agcgacaaca tccgtgttgg gttgattggg tatggttatg
     4021 cgagcaaaac cttccatgca cccctgattg cgggcacgcc cgggctggag ctggcggtaa
     4081 tctccagcag cgatgaaaca aaagtaaaag ctgactggcc aacggttacg gttgtctctg
     4141 agccgaagca tctgtttaac gatcccaaca tagacctgct tgtc
//
LOCUS       DAWDRC010000358         4165 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8921_length_4165_cov_2.406813, whole genome shotgun sequence.
ACCESSION   DAWDRC010000358 DAWDRC010000000
VERSION     DAWDRC010000358.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8921_length_4165_cov_2.406813"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1574..1583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1064 a          998 c         1104 g          989 t
ORIGIN      
        1 cgaaggtatt acacctgacg gacccgcaat tttcaggaca attcaatgaa tcaaatcgac
       61 cgtttgctca ccatcatgca gcgcctgcgc gacccggaaa acggctgccc gtgggataaa
      121 gagcagacat ttgccaccat tgcgccttac acccttgaag aaacctacga ggtgctggac
      181 gccatcgccc gtgaagattt tgacgatctg cgcggtgaac tgggcgatct gctgttccag
      241 gtggtgtttt acgcacaaat ggctcaggaa gaagggcgct ttgactttaa tgatatttgc
      301 gctgctatta gcgataaatt agagcgtcgc catccgcatg tttttgctga cagttctgcc
      361 gaaaatagta gtgaagtgct tgcccgttgg gagcaaatca aaaccgaaga gcgcgcgcag
      421 aaagcgcagc attcggcgct ggacgatatt cctcgtagtt taccggcttt aatgcgtgcg
      481 caaaaaatcc agaaacgttg cgccaacgtt ggttttgact ggacgacgct tggtccggta
      541 gtcgataaag tctacgaaga gatcgacgag gtgatgtacg aggcgcggca ggctgttgtc
      601 gaccaggcta aactggagga ggaaatgggt gacctgctgt ttgccacggt caatctggct
      661 cgtcatttag ggacaaaagc ggaaatcgca ttgcaaaaag cgaacgaaaa attcgagcgt
      721 cgttttcgcg aagtggagcg tattgttgcc gcgcgtggac tggaaatgac aggtgttgac
      781 ctcgaaacaa tggaagaagt ctggcaacag gtaaaacggc aggaaattga tctctaaggg
      841 aattacgcgg tcaagcgcga tttgtgtcat tttttaaatg acaagcgctt gatttgcgtc
      901 aaaaacattt accccaaagg ggctattttc tcactcctga tttcaatagt gcgctggcga
      961 agaggaggga taatgaaagt ttgtggcaca ggtcatgttc gggtatactg ctttcccgtc
     1021 ctggttattc catcgtcttt tcaacctaac ttctcaggtt cagcatgaca acgaactata
     1081 tttttgtgac cggcggggtc gtatcctctc tgggtaaagg cattgccgca gcctccctcg
     1141 cagccattct tgaagcccgt ggcctcaatg tgaccatcat gaaactggat ccgtacatca
     1201 acgtcgatcc aggtactatg agcccaatcc aacacgggga agtgttcgtt actgaagacg
     1261 gcgctgaaac cgacctggac ctggggcact acgagcgttt cattcgtacc aaaatgagcc
     1321 gccgcaacaa cttcaccacg ggtcgtatct actctgacgt tctgcgtaaa gaacgccgcg
     1381 gtgactatct cggcgcaacc gtacaggtta ttccgcacat cactaacgct atcaaagagc
     1441 gcgtgctgga aggcggcgaa ggtcatgacg tagtactggt cgaaatcggc ggtactgtag
     1501 gtgatatcga atccctgccg ttcctcgaag cgattcgcca gatggctgtt gaaattggtc
     1561 gtgagcacac cctnnnnnnn nnnaccccgt ttatgcacct gacgctggtg ccgtacatgg
     1621 cagcgtctgg tgaagtcaaa accaaaccga ctcagcactc tgtaaaagag ctgctttcaa
     1681 tcggtatcca gcctgatatc ctgatttgtc gttcagatcg cgccgttccg gcgaacgaac
     1741 gtgcgaagat tgcattgttc tgtaatgttc cggaaaaagc ggttatttct ctgaaagacg
     1801 tcgattccat ctataaaatt ccgggcctgt tgaaatctca ggggctggac gattatattt
     1861 gtaaacgatt cagcttaaac tgcctggaag cgaatctgtc cgaatgggaa caggttatct
     1921 tcgaagaagc gaatccggta agtgaagtca ccattggtat ggtcggcaaa tacattgagc
     1981 tgccggatgc atacaaatcc gtgatcgaag cactgaaaca cggtgggctg aagaatcgtg
     2041 tcagcgtcaa catcaaactg atcgattcac aagatgttga aacgcgcggc gttgaaatcc
     2101 ttaaaggtct ggacgcaatc ctcgtacctg gcggtttcgg ctatcgtggc gtagaaggca
     2161 tgattacgac cgcgcgtttt gcgcgtgaga acaatattcc ttatctgggc atttgcctgg
     2221 gtatgcaggt ggcgttaatt gattacgctc gccatgttgc caacatggag aacgccaact
     2281 ctacggaatt tgtgccagac tgtaagtacc cggttgtggc gctgattacc gagtggcgcg
     2341 atgaaaacgg caacgttgaa gttcgtagcg agaagagcga tctcggcggt accatgcgtc
     2401 tcggcgcaca gcagtgccag ttggttgacg atagcctggt tcgccagctg tacaatgcgc
     2461 cgacaattgt tgagcgtcat cgtcaccgtt acgaagtcaa caacatgctg ttgaaacaga
     2521 ttgaagatgc aggtctgcgc gttgcgggcc gttccgggga tgatcagttg gtcgagatca
     2581 tcgaagttcc gaatcatccg tggttcgtgg cttgccagtt ccatccggag tttacttcta
     2641 ctccacgtga tggtcacccg ctgtttgcag gctttgtgaa agccgccagc gagttccaga
     2701 aacgtcaggc gaagtaagta aaaaagttag aacggcaacg cgtaccctgg gtacgcgttg
     2761 tttgtctgga gtttcacttt aactagtgac ttgaggaaaa cctaatgtcc aaaatcgtaa
     2821 aaatcatcgg tcgtgaaatc atcgactccc gtggtaaccc gactgttgaa gccgaagtac
     2881 atctggaggg tggtttcgtc ggtatggcag ctgctccgtc aggtgcttct actggttccc
     2941 gtgaagctct ggaactgcgc gatggcgaca aatcccgttt cctgggtaaa ggcgtaacca
     3001 aagctgttgc tgcggtaaac ggcccgatcg ctcaggcgct gattggcaaa gatgctaaag
     3061 atcaggctgg cattgacaag atcatgatcg acctggacgg caccgaaaac aaatccaaat
     3121 tcggcgcgaa cgcaatcctg gctgtatctc tggctaacgc caaagctgct gcagctgcta
     3181 aaggtatgcc gctgtacgag cacatcgctg aactgaacgg cactccgggc aaatactcta
     3241 tgccggttcc gatgatgaac atcatcaacg gtggtgagca cgctgacaac aacgttgata
     3301 tccaggaatt catgattcag ccggttggcg cgaaaactgt gaaagaagcc atccgcatgg
     3361 gttctgaagt tttccatcac ctggcaaaag ttctgaaagc gaaaggcatg aacactgctg
     3421 ttggtgacga aggtggctat gcgccgaacc tgggttccaa cgctgaagct ctggctgtta
     3481 tcgctgaagc tgttaaagct gctggctatg aactgggcaa agacatcact ctggcgatgg
     3541 actgcgcagc ttctgaattc tacaaagacg gtaaatacgt tctggctggc gaaggcaaca
     3601 aagcattcac ctctgaagaa ttcactcact tcctggaaga actgaccaaa cagtacccga
     3661 tcgtttccat cgaagacggt ctggacgaat ctgactggga cggtttcgca taccagacca
     3721 aagttctggg cgacaaaatc cagctggttg gtgacgacct gttcgtaacc aacaccaaga
     3781 tcctgaaaga aggtatcgaa aaaggcatcg ctaactccat cctgatcaaa ttcaaccaga
     3841 tcggttctct gaccgaaacc ctggctgcta ttaagatggc gaaagacgct ggttacactg
     3901 cagttatctc tcaccgttct ggtgaaactg aagatgctac catcgctgac ctggctgttg
     3961 gtactgctgc aggccagatc aaaacgggtt ccatgagccg ttctgaccgt gttgctaaat
     4021 acaaccagct gattcgtatc gaagaagctc tgggcgaaaa agcaccgtac aacggtcgta
     4081 aagagatcaa aggccaggca taagtctgac tttatctgat ttaaaaatgc cagccttcgg
     4141 gctggcgttt tttatggttg agtta
//
LOCUS       DAWDRC010000359         4141 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8977_length_4141_cov_2.135830, whole genome shotgun sequence.
ACCESSION   DAWDRC010000359 DAWDRC010000000
VERSION     DAWDRC010000359.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8977_length_4141_cov_2.135830"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    781..790
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1023 a         1001 c         1117 g          990 t
ORIGIN      
        1 ctgatcgaac aacaatggca ggcgattatc tccgaaggtt taggcgcgca gtatggcgac
       61 gcggtgccgc tgtcactatt gcgtgatgaa ctggcactgc gcctggatca agaacgtatc
      121 agccagcgtt ttctcgccgg accggttaac atttgtactc tgatgccaat gcgttcaatt
      181 ccgttcaaag tggtttgcct gctgggaatg aacgacggcg tttatccacg tcagcttgcg
      241 ccattgggct ttgatctgat gagccagaaa ccgaagcgtg gcgaccgtag ccgtcgcgat
      301 gacgaccgct atctgttcct ggaagcgtta atttccgcgc agcaaaaact ctatatcagc
      361 tatatcgggc gttccattca ggataacagt gaacgtttcc cgtcggtact ggtgcaggaa
      421 ctgatcgact acatcgggca aagccattat ctaccgggcg atgaagcgct taactgtgat
      481 gaaagcgagg caagggtaaa agcgcatctt acttgccacc atacccggat gccgtttgac
      541 ccgcaaaact accagcccgg taacttacaa agctatgcgc gcgagtggct acctgcggcc
      601 agccaggctg gtaaagcaca ttctgaattt gttcagccgc tgccatttac cttaccggaa
      661 accgtgccgc tggaaacgct acaacgattc tgggcacatc cggtgcgggc gtttttccag
      721 atgcgtttgc aggtgaactt ccgtaccgaa gacagcgaaa ttcccgacac tgaacctttt
      781 nnnnnnnnnn tgaatgcact ggttgagcag gatgatgccg aacgcttgtt ccgccgattc
      841 cgggcggcag gtgatttgcc gtatggtgcc tttggtgaaa ttttctggga aacgcagtgt
      901 caggagatgc agcaacttgc cgacagagtc attgcctgtc gccagccggg gcagagtatg
      961 gagattgatc tcgcctgcaa cggtgtgcaa ataacgggct ggttgccgca ggtgcagccg
     1021 gatggcctgt tgcgctggcg tccctcctta ttaagtgtgg cgcagggaat gcaactttgg
     1081 ctggaacacc ttgtctactg tgccagcggt ggtaatggtg aaagtcgcct ttttctacgc
     1141 aaagacggcg agtggcgttt tccgccgctt gcagccgaac aggctttgca ttacctctca
     1201 caactgattg aggggtatcg tgaaggaatg tccgcgccat tgctggtgtt acctgaaagt
     1261 ggcggcgcgt ggctaaaaac ctgttatgac gcgcaaaacg atgccatgct ggatgacgat
     1321 tccacgttgc aaaaagcccg tacgaaattc cttcaggctt acgaaggcaa catgatggtg
     1381 cgtggcgaag gtgatgatat ctggtatcaa cggctctggc ggcaattaac accagagaca
     1441 atggaggcta tcgttgaaca gtcgcaacgt ttcctgttac cgctgtttcg ctttaatcag
     1501 tcatgagtat tgtataaaaa ttgcgcaatc tatccgctta ctttatgatg cgcaccagtc
     1561 acggactgat ggttatatag acataggctg actcgtgcag cacaagatta aattctggca
     1621 gatgatttgc gttaacgtgt tgaatctggc cagaaaataa agttgattat gaggtccgtg
     1681 aatgccccgc agcatctggt tcaaagcatt attgttgttt gttgcccttt gggcaccctt
     1741 aagtcaggca gaaacgggat ggcagcccat tcaggaaacc atccgtaaaa gtgataaaga
     1801 taaccgccag tatcaggcta tacgtctgga taacggtatg gtggtcttac tggtttctga
     1861 tccgcaggca gttaaatcgc tctcggcgct ggtggtgccc gttgggtcgc tggaagatcc
     1921 tgaggcgtac caggggctgg cacattacct tgaacatatg agtctgatgg ggtcgaaaaa
     1981 gtatccgcag gctgacagtc tggccgaata tctcaaaatg cacggcggca gtcacaatgc
     2041 cagcacggca ccgtatcgca cggctttcta tctggaagtt gagaacgatg ccttacccgg
     2101 tgcggtagac cgcctggccg atgctattgc agaacccttg ctcgacaaga aatacgccga
     2161 acgtgagcgt aatgcagtga atgccgaatt aaccatggcg cgtacgcgtg acgggatgcg
     2221 catggcacag gtcagcgcag aaaccattaa cccggcacac cccggttcaa agttttctgg
     2281 tggtaacctc gaaactttaa gcgacaaacc aggtaatccg gtacagcagg cgctgaaaga
     2341 tttccacgag aagtactatt ccgccaatct gatgaaggcg gttatttaca gcaataaacc
     2401 gttgccggag ttggcgaaaa tggcggcgga cacctttggt cgcgtgccga acaaagagag
     2461 caaaaaaccg gaaatcaccg tgccggtagt caccgacgcg caaaagggca ttatcattca
     2521 ttacgtcccg gcgttgccgc gtaaagttct gcgcgttgag ttttgcatcg ataacaattc
     2581 agcgaagttc cgtagtaaaa cggatgaatt gattacctat ctgattggta atcgcagccc
     2641 tggtacactt tctgactggc tgcaaaagca gggattagtt gagggcatta gcgccaattc
     2701 cgatcctatc gtcaacggca acagcggcgt attagcgatc tctgcgtctt taaccgataa
     2761 aggtctggcg aatcgcgatc aggttgtggc ggccattttt agctacctca atctgttacg
     2821 tgaaaaaggg atcgataaac aatacttcga tgaactggcg aatgtgctgg atatcgactt
     2881 ccgttatccg tcaatcaccc gtgatatgga ttacgtcgaa tggctggctg ataccatgat
     2941 tcgcgttcct gttgagcata cactggatgc agtcaatatt gccgatcggt acgatgctaa
     3001 agcagtaaaa gaacgtctgg cgatgatgac gccgcagaat gcgcgtatct ggtatatcag
     3061 cccgaaagag ccgcacaaca aaacggctta ctttgtcgat gcgccgtatc aggtcgataa
     3121 aattagcgca caaactttcg cggactggca gaaaaaagcc gccaatattg cgctttcctt
     3181 accggagctt aacccctata ttcctgacga tttctcgctg attaagtcag agaagaaata
     3241 cgaccatcca gagttgattg ttgatgagtc gaatctgcgc gtggtgtatg cgccaagccg
     3301 ttattttgcc agtgagccca aagctgatgt cagcctgatt ttgcgtaatc cgaaagccat
     3361 ggacagcgcc cgcaatcagg tgatgtttgc gctcaatgat tatctcgcag ggctggcgct
     3421 tgatcagtta agcaaccagg cgtcggttgg tggcataagt ttttccacca atgctaacaa
     3481 cggccttatg gttaatgcca atggttacac tcagcgcctg ccgcagctgt tccaggctct
     3541 gctggagggc tactttagct ataccgctac ggaagatcag cttgagcagg cgaagtcctg
     3601 gtataaccag atgatggatt ccgcagaaaa gggcaaagcg tttgagcagg cgattatgcc
     3661 cgcgcagatg ctctcgcaag tgccgtactt ctcgcgagat gaacggcgca aaattttgcc
     3721 ctccattacg ttgaaagagg tgctggccta tcgcgacgcc ttaaaatcag gggctagacc
     3781 agagtttatg gttatcggca acatgactga agcccaggca acaatgctgg cacgccatgt
     3841 gcaaaaacag ttgggcgctg atggttcaga gtggtgtcgt aacaaagatg tcgtggtcga
     3901 taaaaaacaa tccgtcatct ttgaaaaagc tggtaacagc accgactccg cactggcagc
     3961 ggtatttgta ccgactggct acgatgaata caccagctca gcgtatagtt ctctgttggg
     4021 gcagatcgta cagccgtggt tctacaatca gttgcgtacc gaagaacagt tgggctatgc
     4081 cgtgtttgcg ttcccaatga gcgtagggcg tcagtggggc atgggcttcc ttttgcaaag
     4141 c
//
LOCUS       DAWDRC010000360         4132 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_8994_length_4132_cov_2.425803, whole genome shotgun sequence.
ACCESSION   DAWDRC010000360 DAWDRC010000000
VERSION     DAWDRC010000360.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8994_length_4132_cov_2.425803"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    263..284
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1097 a         1094 c         1058 g          861 t
ORIGIN      
        1 ttttcagcca ttgcataaat aatccccgca tccattaatg ccggataccg gcgcggtaac
       61 gtggcagcaa aatcagaaag tttattaagc atccctggca tatcgcggat taagtagatg
      121 ccctgctgcc aggcaatagg cagaaccacg aacgccatca gtagcaggat gcccacaaac
      181 aggatcagca cgatagatgt cgcccaaggg cgggaacagc caatagactg caggcggaca
      241 gtcggccatt ccagcaaata ggnnnnnnnn nnnnnnnnnn nnnnttccct gaacctcaag
      301 agcgggattg cgatccgcaa ttttatcgaa atgtcacaaa aaagacttcg ctttttatga
      361 cggattcagg aaactgagaa gtcatttgag tgggctaatc ttcgccgtta cactcaaagg
      421 cggcgcggtg ggaacgatat ttcacagtat cggtcaaatg actatctcca gaaatacagg
      481 atagaggtta tgttcaggca gttgaaaaaa aacctggttg caaccctcat tgctgctatg
      541 accattggtc aggtagcccc ggcgtttgcc gacagcgcag acaccttgcc ggatatggga
      601 acctccgcag gaagcacact ttccattggt caggaaatgc agatgggcga ctattatgtc
      661 cgccagttac gcggcagcgc gccgttaatt aatgatccgc tgttaacgca atacattaat
      721 tcgttaggaa tgcgtctggt ttcgcatgcc aattcggtta agacaccgtt tcatttcttt
      781 ctgatcaaca acgacgaaat taacgccttt gctttctttg gcggcaacgt ggtgctgcac
      841 tctgccctgt tccgttattc cgataacgaa agtcaactgg cttcagttat ggcgcacgaa
      901 atctcccacg tcacccaacg tcacctggcg cgagcgatgg aagatcagca gcgcagcgcg
      961 ccgctgacct gggtcggcgc gttaggttct attttactgg cgatggccag tccgcaggcg
     1021 gggatggcgg cgctgaccgg tacactggcg ggaacgcgcc aggggatgat cagtttcacc
     1081 cagcaaaatg aacaggaagc ggaccgcatt ggtattcagg tgctgcaacg ctcgggattc
     1141 gatccgcagg cgatgccgac cttcctcgaa aaattactcg atcaggcgcg ttactcttcg
     1201 cgcccaccag aaattctgct cactcaccca ctaccggaaa gccgtctggc tgatgcccgt
     1261 aaccgtgcca atcagatgcg cccgatggtg gtgcaatcgt cagaagattt ctatctggca
     1321 aaagtgcgca cactggggat gtataattcc ggacgtaatc agctcaccag tgatttgctg
     1381 gatgaatggg cgaaaggaaa cgttcgtcag caacgagcgg cgcaatatgg tcgtgcttta
     1441 caggcgatgg aagccaataa atacgacgag gcgcgtaaaa cgctgcaacc gttactggca
     1501 gcagaacctg gcaacgcatg gtatctcgat ctggctaccg atatcgatct tgggcaaaac
     1561 aaagccaatg aggcgatcaa tcgcttgaaa aatgcccgtg atttgcgcac caatccagtg
     1621 ttgcagctca acctggcgaa cgcttatcta caaggcggcc aaccacaaga agcggccaat
     1681 attctgaatc gctacacttt taacaataaa gatgacagca acggctggga tttactggca
     1741 caggcggaag ccgcactaaa taaccgcgat caggaactgg ctgcgcgagc agaaggttat
     1801 gcgctcgccg ggcgactcga tcaggccatt tccttgttga gtagcgccag ttctcaggtg
     1861 aaattaggca gcctgcaaca agcgcgttac gatgcgcgca tcgaccagtt gcgccagctg
     1921 caggaacgct ttaagcctta taccaagatg taattcgcat taaaggaaga acaatgacca
     1981 aacaggtaaa aatctaccat aacccacgct gttcaaagag ccgggaaacg ctgaacctgc
     2041 tgaaagaaaa cggcgtggag ccagaagtgg tgctctacct tgagacaccc gccgatgcgg
     2101 caacgctgcg tgatttgctg aaaatgctgg gaatgaatag cgctcgtgaa ttgatgcgcc
     2161 agaaagagga tctttataaa gaactgaacc tggctgacag ttcgcttagc gaaggggcgc
     2221 tgattcaggc aatggtggat aatccaaagc taatggagcg cccgattgtg gtcgcgaatg
     2281 gcaaagcgcg gattggtcgt ccaccggagc tggtactgga aatcgtgggt taagaagtgc
     2341 tccatcgcta gttgaagcac attgccggat gcgacgcttg ccgcgtctta tccggccgac
     2401 gaatactatg ggtgcgcatc caacaataaa cgtcttgtct atagtttcag aatttctttc
     2461 acaaacggaa tggtcagctt acgttgcgcg gtaatcgacg cacgatccag ctgatccagc
     2521 gtcataaata acgtgcgcat ttctctgtcg agccgcttca gcaagaaacg ccccacatct
     2581 tccggcagtt caaaaccacg caaacgcgcg cgtaactgta gcgcctgcaa cttatcttca
     2641 tcagaaagtg gctgcaattt gtagatctgc ccccagtcga gacgcgacgc gagatccggt
     2701 aatcccagat tcaactgccg cggtggacga tcgccggtga tcaacaaccg tgttttgccc
     2761 gattccagaa ttcgattgta gagatcgaaa atcgccatct cccacaactc atcgcctgca
     2821 atacactcaa tgttgtcgat acagaccagc gacaaatgct ccataccgtc gagcacttcc
     2881 ggaacaaacc aggtgcgttt atccagcggg acatagccca ccgcatcgcc acgctgtgac
     2941 aattccgcgc aagccgcgtg cagcagatgg ctgcgccccg cgccttcgcg tgcccagaga
     3001 tagatgtaac cgctatgttc ctgacgcagc acgttttgca gcgcagccag taaagaggag
     3061 ttatcccccg gccagaaact tgcaaaggtt tcgtcgtcag gaagataaag tggcaaagag
     3121 agctgtgccg gtgtgttcag agatacctca accaggattt cacaaaatcg cgagaagttt
     3181 accacagaat cccatgatgt ttgaaccggg cagcaacact gcccggtcgg tacattattt
     3241 gtctgttata tccgcgtctt ctgcgtccag caccacttct tcagggcgca gcatactaat
     3301 cagtttgaag atcaaactta agccgatacc gacgatggtc gccagcgcca tacctttcag
     3361 ctccgccgcg ccaatgttta ccttcgcgcc actgacgccg atgatcaaaa taacggaagt
     3421 caggatcagg ttctgtgctt tgttgtaatc cactttcgat tcgatcaaca cacgaatacc
     3481 ggaagcaccg atgacaccgt aaagcagcag cgaaacgccg cccatcaccg gcaatggaat
     3541 catctggata gcagcagcca gtttaccgac gcaggaaagc aggatagcga aaatcgccgc
     3601 accgccgata acccaggtac tgtaaacacg ggtgatcgcc atcacgccaa tgttttctcc
     3661 gtaagtggtg tttggcgtgg agccaaagaa gccggagatc acggtcgaca agccgttagc
     3721 aaacatcgaa cggtgcagac ctggatcgcg cagcagatct tttttgacga tattagccgt
     3781 tacgaccagg tgccctacgt gttcggcaat aaccactaac gccgctggca gaatagtcag
     3841 aatggcaaac cactcgaagc gcggcgtata gagagttggc agcgcaaacc agtgagcatt
     3901 aataatcggc gtggtatcga caattcccat tgcgaaagag agcgcgtagc ccaccagcac
     3961 gccaattaaa atcgggataa ttgccaggaa accacgaaac agcacggaac ctaaaaccgt
     4021 gaccgccagg gtggtaatag agatgatgat ggttttggag tctggcgttt gcccttcagc
     4081 cgggagtaaa cccgccatac cggcagctac gcccgccaga tccagcccga tg
//
LOCUS       DAWDRC010000361         4120 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9011_length_4120_cov_1.693481, whole genome shotgun sequence.
ACCESSION   DAWDRC010000361 DAWDRC010000000
VERSION     DAWDRC010000361.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9011_length_4120_cov_1.693481"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3124..3133
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1001 a         1081 c         1147 g          881 t
ORIGIN      
        1 gcatggcctg cgaacagccg gacaacggca gcaaccatcc gcgtaacctg tttgtctggc
       61 gttcaaacct gcttggctct tccggcaagg gccacgagta tatgcagaag tatctgctgg
      121 gtacgaaaag tggcattcag ggggaagaac ttggaccgac ggagggaatc caaccggaag
      181 aagttgagtg gcaaactgcg gcgattgaag gcaagctcga cctgctggtg acgctcgatt
      241 tccgcatgtc cagtacctgc ctgttctccg atatcgttct gcccaccgcc acctggtacg
      301 aaaaagacga tatgaatacc tcggatatgc atccgtttat tcatccgctt tctgcggcgg
      361 tcgatccggc ctgggaatca cgcagcgact gggaaatcta caaaggtatc gctaaagcct
      421 tttcgcaagt gtgcataggg catctcggca aagaaaccga cgtggtatta caaccactgc
      481 tgcatgactc cccggcagag ctttcacagc cgtgtgaagt gctcgactgg cgcaaaggcg
      541 agtgcgatct gattccaggc aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc
      601 ctgctaccta tgaacgcttt acctcactcg ggccattgat ggacaaactt ggcaacggcg
      661 gtaaagggat ttcgtggaat acccaggatg aaatcgattt cctcggtaaa cttaattaca
      721 ccaagcgtga tggcccggca caggggcgtc cgctgattga caccgccatt gacgcatcag
      781 aagtgattct ggcactggca ccggaaacca acggccatgt tgcagtcaaa gcgtggcaag
      841 cgctgggcga gatcaccgga cgcgaacata cccatctggc gctgcacaaa gaggacgaga
      901 agattcgctt tcgcgatatt caggcacagc cacgtaaaat tatctccagc cccacctggt
      961 ctggtctgga aagcgatcac gtctcctata acgcgggata caccaacgtt catgagttaa
     1021 ttccgtggcg cacgctgtcg ggacgccagc aactctatca ggatcatccg tggatgcgtg
     1081 cttttggtga aagtctggtg gcttatcgcc cgcctatcga cacccgtagc gtcagtgaaa
     1141 tgcgccagat accgccaaac ggcttcccgg aaaaagccct taacttcctg acgccgcacc
     1201 agaaatgggg cattcactca acctacagtg aaaacctgct aatgctgacg ctctctcgcg
     1261 gtggaccgat tgtctggatc agcgaaaccg atgcccgaga actgaccatt gtcgataacg
     1321 actgggtgga agtatttaac gccaacggcg cgctgactgc ccgcgcggtg gtcagccaac
     1381 gtgtaccgcc gggcatgacc atgatgtatc acgcccagga acgcattatg aatattcctg
     1441 gttcggaagt aactggcatg cgcggcggca ttcataactc ggttacccgc gtttgcccga
     1501 aaccaacgca tatgattggc ggttacgcgc agctggcctg gggctttaac tactacggca
     1561 ctgtcggctc gaaccgcgac gaattcatta tgatccgcaa aatgaagaac gttaactggc
     1621 tggatgatga aggtcgcgat caggttcagg aggcgaaaaa atgaaaatac gttcgcaagt
     1681 cggcatggtg cttaacctcg ataaatgtat cggctgccat acctgttcgg tgacctgtaa
     1741 aaacgtctgg accggacgcg aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa
     1801 accgggcatt ggttatccga aaaactggga agatcaggat gagtggcaag gtggctgggt
     1861 gcgtgatgtg aatggcaaga tacgcccgcg tctgggcagc aagatgggcg taataaccaa
     1921 aattttcgcc aacccggtag tgccgcagat tgacgattac tacgaacctt tcaccttcga
     1981 ctacgaacat ttgcatagcg cgccggaagg caaacatatt cccacagccc gtccgcgttc
     2041 actgattgac ggcaaacgga tggacaaagt gatctggggc ccaaactggg aagaactgtt
     2101 gggcggcgaa ttcgaaaaac gcgcccgtga ccgcaacttc gaggccatgc aaaaggagat
     2161 gtacgggcag tttgaaaaca ccttcatgat gtacctgccg cgcttgtgcg aacactgcct
     2221 caatcccagt tgcgtggcga cctgcccaag cggcgctatc tacaaacgcg aagaagacgg
     2281 cattgtgctg atcgatcagg ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc
     2341 gtacaaaaaa atctacttca actggaaaag cggcaagtca gaaaaatgta tcttctgtta
     2401 cccgcgaatt gagtcaggcc aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg
     2461 gtatctgggc gtgctgcttt acgacgccga ccgcatcgag gaagcggcga gcaccgagcg
     2521 cgaagttgac ctctatgaac gccagtgcga agtgttcctc gatccgcacg atccctcagt
     2581 gatcgaagaa gccctgaaac aaggtattcc acaaaacgtg attgacgctg cccagcgttc
     2641 gccagtctac aaaatggcaa tggactggaa actggcgcta ccgctgcacc ctgaataccg
     2701 caccctgccg atggtctggt acgttccgcc gctgtcaccg attcagtcct acactgatgc
     2761 gggaggtttg ccgaaaagcg aaggcgtgct gcccgccatc gaaagcctgc gtattccggt
     2821 gcaatatctc gccaatatgt tgagtgccgg tgataccggt ccggtactgc gggcgctgaa
     2881 acggatgatg gcgatgcgcc actatatgcg ttcacaaacc gtggaaggcg ttactgatac
     2941 tcgtgccatc gacgaagtag gtctgagcgt cgcccaggtc gaagagatgt atcgttacct
     3001 cgccattgcc aactatgaag accgttttgt cattccaacc agtcatcggg aaatggcggg
     3061 cgatgccttt gctgaacgca acggctgcgg ttttaccttt ggcgacggtt gccatggctc
     3121 ggannnnnnn nnncaaattc aacctgttca acagtagccg tatcgatgcc atcaacatca
     3181 ccgaagtgcg cgacaaagcg gagggcgaat aatgcagatc ctcaaagtga tcggcctgtt
     3241 gatggagtat ccggacgagc tgttgtggga atgcaaggag gacgcgctgg cgttgatccg
     3301 ccgcgacgca ccgattctta cggattttac ccgcaacctg cttaacgcgc cgctgctgga
     3361 taaacaggcc gaatggtgcg aagtgtttga ccgtggacgt accacatcgc tgctgctgtt
     3421 cgaacatgtt catgccgagt cccgcgatcg cggccaggca atggtggacc tgctggcgga
     3481 gtatgaaaag gtcggtctgc aactgaattg tagggaactg cccgattatc taccgctgta
     3541 tctggagtat ttaagcgtac ttccggacga tcaggcgaaa gaaggattac tcaacgttgc
     3601 gccgatcctc gccctgcttg gcggacgctt aaaacaacgc gaggcaccgt ggtacgcgtt
     3661 atttgacgcc ctgttgcaac tggcaggaag ccctctgtca agtgacagtg tcacaaaaca
     3721 gattcgcagt gaagagcgtg acgacaccca ccaggcgctg gatgcggtgt gggaagaaga
     3781 acaggtgaag tttattgaag ataacgccac ggcgtgtgac agctcgccgc ttaatcaata
     3841 tcagcgacgc tttagccagg atgtcgcgcc gcagtatgtc gacatcagtg cgggaggtgg
     3901 gaaatgattc agtatctgaa cgtctttttt tatgatatct acccgtactt gtgtggcacg
     3961 gtttttatcc ttggaagctg gctgcgctac gattacgggc agtatacctg gcgcgccgcc
     4021 tccagccaga tgctcgataa acgtgggatg gtgatatggt cgaatctgtt ccatatcggc
     4081 attttgggga ttttcttcgg gcacctgttc ggcatgttaa
//
LOCUS       DAWDRC010000362         4085 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9094_length_4085_cov_1.940199, whole genome shotgun sequence.
ACCESSION   DAWDRC010000362 DAWDRC010000000
VERSION     DAWDRC010000362.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9094_length_4085_cov_1.940199"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    953..962
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3712..3721
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          873 a         1167 c         1078 g          947 t
ORIGIN      
        1 aatcgtccgg cgtgccgcta ggtattaatt tcccgcggta caccaggccg atgcggtcgc
       61 aatattccgc ttcatccata aagtgggtgg tgaccatcac cgtgacgcct ttctctacca
      121 tactgttgat atgtagccaa aattcacggc gggtgagggg atcaacgccg gacgtcggtt
      181 cgtcgagaaa cagaatgtcc ggttcatgca tcagcgaaca ggccagcgcc agccgctgtt
      241 taaaacctaa tggcagttca tcggtcgcgt gggaggcgat acttttcagg ccgaacgcct
      301 cgctcatacg ggagattttt tcgttctgcg cccgaccgcg taagccatac acaccagaga
      361 aaaagcgtaa attctgttcg accgtcaggt tgccgtagag cgaaaatttt tgcgccatat
      421 agccgagatg ctggcgcgct ttaccggaac tctctttcag atccatcccc agcaccagcg
      481 cctggccgga agtcggcacc agcaaaccgc acatcatctt aaaggtggtc gatttgcccg
      541 cgccgtttgg ccccagcaaa ccaaaaatct ccccgcgttt aacggcaaag ttgacgtgat
      601 cggtggcggc aaaatcacca aatttcttgg tcagttcttt cgcttcgatc accgtctcgc
      661 caggtgtacc ttccaccgta tgcaatattg cgcccagcgg cgattccgag gttccggcac
      721 cgcccagcaa atcaataaac gcatcttcaa aacgtggcgt agtttcgtta atgttgattt
      781 ccggcatccc gtcggcatgg cgaatatcgt ctggtgtagc ctcttttttg aggatcagac
      841 gtaccgattt cccctgaatc atgccgtcgc tgacctgcgg cagtttcaat gcccgttgca
      901 acagtttgcg gttgccctcg tgcgggctgg tcatcagaac gctgcgtccg tcnnnnnnnn
      961 nnccagggta caggccaggc cgagtttttg tttcatccca ccggagagct tgcccgccag
     1021 gcgtccggta aacgggccaa gagacgtaaa ctccagcagg cgagcaaaag tctgcttccg
     1081 tgcttcaccg gtgacgctgc gcaaatccgc gtacagattg aggttctcca tcaccgtcag
     1141 atcttcatac aggccgaatt tctgcggcat atagccgagc acggcgtgca gcgcagcgtc
     1201 atttttgatc ggatcaaagc caatcaccgt ggcactgccg ctgtcgggtt tcagtaatcc
     1261 cgccagcatc cgcatcagcg tggttttgcc cgcaccgtcc ggcccaacca gccccgtcac
     1321 ataaccggcg tgaatggtgc agtcgagcgg tgcgacggcg ggcttgtcca tgcccggaaa
     1381 gcgtttttcc aggccgttca gcgtgataac ggcatcattc atgtcctgcc tcgtcaccga
     1441 attgcaccgt taccggcatt ccctggcgta acgcatcatc ggcgtcgttc accacaatac
     1501 gcaggcgata gacgaggtcg gtacgcagat ccggtgtttc gacggttttc ggggtaaatt
     1561 cagcagtcgg cgaaacgaaa ccaatctgcc cgtgatacgg cttgtccggg cgaccatcgg
     1621 tataaagcaa cactttgcgc cccggctggg cctggtcaag attacgttca tcaacatagg
     1681 cgcgtaccca caccggatgc gtcagcgaga cggtaaatac cgtgccacct tcattaagca
     1741 ccgtgcctgg ctccaccgcg cgcgttaaca gcgtgccatc agacggggct atcaacgttg
     1801 agtcctgtaa attcaactcc gcctgcgcca gttgcgcctg cgcctgttcg aggctggctt
     1861 tcgcctgagc gatgtcctgt tcacggttac cggaacggta ctgacgcaat ttatcctgtg
     1921 ctgatttcag cgttgcctgc gcctggtcgc gcgaggagcg ggcattttcc aggtcatttg
     1981 ccgaaatggt gcggcttttc cacaaccctt gctggcggtt gtagaagttc tgcgcatagt
     2041 cataggcggc ctgcgcctgt tttacggctg cggcggcctg agcgatttct tcatcgcgat
     2101 acccggcaag catcaggtca tactgcgcct gcgccaccga aacacccgct ttcgcctgca
     2161 tcagggcaat ctcatacggc ttgtgatcca gttcgcccag cacctggccc gctttgatag
     2221 catcaccttc gtccaccgcc agcgattcaa cgcgcccccc aacacggaaa ctaagattca
     2281 ccgtacgaat atccacgttg ccatacagcg tcaggccgtt atcctggcgg ctttgatacc
     2341 accagtagcc tccggcaacc acggcggcaa gtaccactac cgccagtccg atcacgacag
     2401 gttttttcat cactacaaac tcctttgcga taatccttgc aaaatcaggt cgatatgaca
     2461 ggtcaccgtc tggttgatca gttcggtctt ttcttcatcg aacgcggtcc agccggtacg
     2521 taacaaaata gtttctttgc caagacgaaa cgccagaatc tcaccaatca acgcatgggt
     2581 atggaggatc atgcgggtgt cgttggcgtc gcagccggtc caggcggcaa tcaggcgtgt
     2641 aaggtggctg tgtagcgggc taatcacctg ttcatgcacc aggtggtagg ctgccgtagg
     2701 agagagttgc tcacgggaga taaacttgct gagattgacg gtgtcatcct gggtgagcag
     2761 cttaatcatg ttcctgcagg cgcgaaggat cagttcacgg atagctgcgc gatcaggctg
     2821 tggttgcgcg aacaaacgtt cggcttcttc ggcgtgcgga cggaactgct cgccaataaa
     2881 atcggcaatc cactgggcgc aggcgaggta caaatcttct ttcgaaccga agtagtaggt
     2941 gatggcagcg atattctgcc cggcctgggc ggctatctcg cgggtggtgg cgttcattcc
     3001 atattcacca aactgtgcca gtgccgcagc aatcagctgt tttttcgcct gttcaccctt
     3061 gattgtcatg gcaggattat tcatcgcaca gtccattctt aatcaaatga ttgattaaga
     3121 ttatgactcc ataggggagt tgtccagtat ggctaagaat tttagctacg ccagtcacag
     3181 ggataattta tgcgctgcgt cacaaaaact gctacactcc gctccctcat gacattgtgg
     3241 tttttgtcat tttccttttc agtatctccc tgaaaactac accggtaacg gtcggggcgg
     3301 ttcggagtag ttatgtcttt cgattctttg ggtttaagcc ctgatatcct gcgcgccgtt
     3361 gccgagcagg gttaccgtga acccacccct attcagcagc aggcgatccc tgcggtgctg
     3421 gaaggccgcg acctgatggc cagtgcccag accggtaccg gcaaaacagc gggctttacg
     3481 ctgccgctgt tgcaacacct gatcactcgc cagccgcacg ccaaagggcg tcgtccggta
     3541 cgtgcgctca ttcttacccc gacccgtgaa ctggcggcgc agattggcga aaacgtccgt
     3601 gattacagca aatacctgaa tatccgttcg ctggtggtgt ttggcggtgt cagcattaac
     3661 ccgcagatga tgaagttgcg cggcggcgtt gatgtgctgg tggcaacgcg gnnnnnnnnn
     3721 nggttgaaat cctcgtcctc gatgaagctg accgcatgct cgacatgggc tttatccacg
     3781 atatccgtcg cgtgttaaca aaactgccag cgaagcgcca gaacctgttg ttctccgcga
     3841 ccttctctga cgatattaaa gctctggcgg aaaaactgtt gcacaacccg ctggaaatcg
     3901 aagtggcacg ccgcaatacc gcgtctgatc aggtgaccca gcatgttcac tttgtcgata
     3961 agaaacgcaa acgcgaattg ctgtcgcaca tgattgggaa agggaactgg cagcaggtgc
     4021 tggtgtttac ccgtaccaaa catggcgcta accatctcgc tgaacagctc aataaagatg
     4081 gcatc
//
LOCUS       DAWDRC010000363         4078 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9107_length_4078_cov_2.358190, whole genome shotgun sequence.
ACCESSION   DAWDRC010000363 DAWDRC010000000
VERSION     DAWDRC010000363.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9107_length_4078_cov_2.358190"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    264..273
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    867..876
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3502..3511
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          951 a         1041 c          947 g         1109 t
ORIGIN      
        1 gtcaacccat gctcatggaa gattattttt tgagcacaca accgcagcta acaccttaca
       61 attaacaaat ttgcatcaat ccgccatcaa tttacacaca ttattatgtg atatttgcca
      121 accgctaaat atgcgttttg ttatctacgc ataaaaacag caacttcaat gtcttaatgg
      181 cagtttttct tgattttaat cagcattcac cgccaattta ttgggcatat tttttcctta
      241 acttttagga attttttatt attnnnnnnn nnnaaaaatt atgctgacat tcattgaact
      301 ccttattggg gttgtggtta ttgtgggtgt agctcgctac atcattaaag ggtattctgc
      361 cactggcgtg ttatttgtcg gtggcctgtt attgctgatt atcagtgcca ttatggggca
      421 caaagtgtta ccgtccagcc aggcttcaac aggctacagc gccacggata tcgttgaata
      481 cgttaaaata ttgctaatga gccgcggcgg cgacctcggc atgatgatta tgatgctgtg
      541 tggctttgcc gcttacatga cccatatcgg cgcgaatgat atggtggtca agctggcgtc
      601 aaaaccattg cagtatatta actcccctta ccttctgatg attgccgcct attttgttgc
      661 ctgtctgatg tcactggccg tttcttccgc aaccggtctg ggtgttttgc tgatggcaac
      721 cctgtttccg gtgatggtaa acgttggtat cagccgtggt gcagctgctg ctatttgtgc
      781 ctccccggcg gcgattattc tcgcaccgac ttcaggggat gtggtgctgg cggccaacta
      841 ccagctggtg gccagggccc acctcannnn nnnnnngcgc aggcttccga aatgtcgctg
      901 attgacttcg ccttcaaaac gacgctgcct atctcaattg ctgcaattat cggcatggcg
      961 atcgcccact tcttctggca acgttatctg gataaaaaag agcacatctc tcatgaaatg
     1021 ttagatgtca gtgaaatcac caccactgcc cctgcgtttt atgccatttt gccgttcacg
     1081 ccgatcatcg gagtgctgat ttttgacggc aaatggggtc cgcaattaca catcatcact
     1141 attctggtga tttgtatgct aattgcctcc attctggagt tcatccgcag ctttaatacc
     1201 cagaaagttt tctctggtct ggaagtggct tatcgcggta tggcagatgc atttgctaac
     1261 gtggtgatgc tgctggttgc cgctggggta ttcgctcagg ggcttagcac catcggcttt
     1321 attcaaagtc tgatttctat cgctacctcg tttggttcgg cgagtatcat cctgatgctg
     1381 gtattggtga tcctgacaat gctggcggca gtcacgaccg gttcaggcaa tgcgccgttt
     1441 tatgcgtttg ttgagatgat cccgaaactg gcgcactcct ccggcattaa cccggcgtat
     1501 ttgactatcc cgatgctgca ggcgtcaaac ctgggtcgta ccctatcacc cgtttctggc
     1561 gtagtcgttg cggttgccgg gatggcgaag atctcgccgt ttgaagtcgt aaaacgcacc
     1621 tcggtgccgg tgcttgttgg tttggtgatt gttatcgttg ctacagagct gatggtgcca
     1681 ggaacggcag cagcggtcac aggcaagtaa atagtaatgc cggaggcaag tttcctccgg
     1741 ctttatcatg tcacccactg tggtatatgc gttgcggtct gccaactttg ccgtgaacaa
     1801 tttcagcaat aatcagatgg cggctggcgc aatattcaag ataacgcctg gcagtggtgc
     1861 ggctgatggt cagtgcctgc gccaccgttt ctgccgtatg ttgcacacca ggctctttga
     1921 acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc ggtcggcagt tcgtctttgg
     1981 gttcaccgcg agcataggca ttaaacatct catcaatttg cttctggctg gcgctatcaa
     2041 tactttccag catatgttta cgctggcgga aacgggttag cgtttgcccc aaccgttcat
     2101 aggcaatggg cttaatgaga taatcaaata caccacaacg tacggcttca gacaccgttt
     2161 ccatatcgct ggctgcagtg gtaaacacca cgtcgccggg ataatgcgcc tgcaccagtt
     2221 catgcagtaa attaatccct ctaccgtcag gaagatagtt atcaagcaag attagccccg
     2281 gcttaaaacg ctcgatcatc attcgggcct gcgccaggtt tcccgccagt aatatctggc
     2341 tgaatccggg aatgtgacga atatattccg catgcatctc tgccagcggc gtttcgtcct
     2401 caacgatcaa tagggttaat ggagctgtca ttaggtttca ctttcggaat atagattgaa
     2461 aataaggtac cgcagggatc attatcttcg agagtgataa caccaccgca gcgcgttacg
     2521 tagctggcaa tcaagtacaa cccaatgcca tgttcaccgg gctcgtcagc acgcgtactg
     2581 acaccctgct caaatatttt gtctcgtaga gactctggaa cgccgcagcc ctgatcggcg
     2641 acttcaatca ccacatcatc gccttcatcg ctgaggtata attcaacgat cttgtttcct
     2701 tcatcgctac gcaggcttgc ttcgaaggcg ttatcaagta aattgccaac aatggctgca
     2761 aactcggtgc tgtccagtcc tggcggcagt tgcgaaagct ggctaccggg gacgatgacc
     2821 atttttagcc ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc cgccacctgg
     2881 cgatcggcaa acgcctcacg caggctgtca ataagctgtt gctgggcctg agactccccc
     2941 tgcaccatcg ccagcacgcg atcatactct ttcatctgca acagaccatt gagcgtcgac
     3001 atccaattga gatgctcgtg tcgcaatgta cgaaggctct cgacgtattg tttaatttgc
     3061 gtcagttgcg cattgagggt ggaaatttcg tctttactac gaaagctaat aatggccccc
     3121 agtaaatcat cgccagaacg aatagcttcc cggttggcaa taacgctcag accgttaaag
     3181 ttcgccacca catcctgacg tttttcatca atctgttcgg taaagaaatc ggcgggcctg
     3241 accacttcag caatgggttt acccaaccat tgccgtccgg gggaactcaa ccccagcatc
     3301 tttcttgcgt tacgattgat ggcggtaatg tacccgtgcg gatccaccgc aatcagccct
     3361 tcataaaccg aactaaacag cgcctcttgc tgacggacca cgcgagcgat ttgctttggc
     3421 tccatgccca tcatctgccg acggatatgc gcggccagga accacgacag caacatcaaa
     3481 atccctaaca gcacgacaaa cnnnnnnnnn ncgacaccac gccgatgact tttccatcgt
     3541 catcaaagat tggcgttttg gcacgcatcg ccatgccaat tgaccctttc ccggtgatga
     3601 agtagctctc ccctttctcc agcgcgcccg gtttggtgaa ctgcatagga taaccaattt
     3661 tctccggatt aggatggtaa agacgaatcg agtgccgatc gccaatcacc acataatcga
     3721 aatcagtatc tctttgtaat ttgtcagcga tggtcgccag cctcttgtag tcacgcgttt
     3781 tcactgcact gatgatactg tcattagaag caataatttt tgcctgattc attgccatgt
     3841 cgcgcacatg aagcgttaaa tagtcctcaa aactggctgt aaaatattgc gccagggctg
     3901 caatgacaaa tattgagaac accagaatca gcaaaaagat acgcagcgga aatgccagtc
     3961 tttggaaaaa tgcaaactgt ttattctctt taagctgcaa cattgttttc cttgcgggtt
     4021 gtcccgagcc tctttgacgg atgtaattaa aatgttgaat caaaatacat agatatta
//
LOCUS       DAWDRC010000364         4073 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9119_length_4073_cov_2.494525, whole genome shotgun sequence.
ACCESSION   DAWDRC010000364 DAWDRC010000000
VERSION     DAWDRC010000364.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9119_length_4073_cov_2.494525"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1144..1153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          969 a         1110 c         1017 g          967 t
ORIGIN      
        1 cttagctcag tggttagagc aggcgactca taatcgcttg gtcgctggtt caagtccagc
       61 aggggccacc agacatagca aaggctgacg agaaatcgtc agcctttttc tttttatata
      121 tcagttactt tgcgtgccag taagccgccg cgcgtacccg ctgtgggtca tactgttccg
      181 cttcaaagcg acggcttaaa ttcttaacga ctttgccttc gccggttatc cagatgaagt
      241 aatcatcggc agggatttgc atctgcgcca gacgcgcatc taccgcctgc tcatcatgtg
      301 ccagccattc gatattaaaa ccatcaaggt gcgcgagata atcctgacag gcgttatccc
      361 gcacgctaac cagcgcacta acttgcggtt taacggcaag tttgctcaac gtttccaggc
      421 ggcggcgcag tgcgggcatt ccggattcat cgcagacata cagctgatac gcgtaatcct
      481 ctggcaccac cagcgaaccg cgcggacctg ccaccgtaag tttatcgccc ggtttcgcct
      541 gcatcgccca gccgctggcg accccaccgt cgtgaataaa gaaatcgatc gccagttcat
      601 ggcgtagttc gtcatacagc ggcgtatagt cacgcgacgg tgggcgtggt ccttccggcc
      661 agacgatgcc ctcttccgtt accgttggcg gcacaaagtg agtgcctggt tgaggaaaga
      721 agagcttgct gtgatcgtca aagccgcgcg atgtaaaacc gtccagcgcc tcgccgccaa
      781 ggacaatgcg ctgaaaaccg gcgctgatac gctcaacgcg tagcacagtc agttcacgga
      841 agcgcagatc attgcgaacg cgctgcgggt agcgggggga gttattcatt gttatcgcct
      901 tcatgatggt aatcagatat atctaaataa attccacaaa tgataatgat tgttaatcat
      961 gataaatgca agtgatttgt agcactgata tatctgtagt ctgataagac gcgcaacctc
     1021 ttatcaagca tcattgctca atgccggatg cggcgtgaac gccttatccg gcctacatgt
     1081 tgagtaaacc tgcgtttaaa aatcattttt gtacttgcag taatgcttat atcagatata
     1141 aatnnnnnnn nnnaagggtg ttgtaaacat gaaggccagc cacgccatga gggctgctgc
     1201 aaaggtgaga agtcagaaca cgagcactgc ggacacggtc accagcatga acacggtcaa
     1261 tgctgcggtg gtcgccacgg tcgcggcggc ggtcgtcgac aacgtttctt tggtcacggt
     1321 gaattgcgtc tggtgattct ggatattctc tcgcgcgatg acagccacgg ttacgagttg
     1381 attaaagcga ttgaaaatct gacccagggg aattacaccc caagcccggg cgtcatctac
     1441 ccgacgctgg attttctgca ggagcagtcg ctgattacca ttcgcgaaga ggaaggtggt
     1501 aagaagcaga ttgcgctgac cgaacaaggc gcgcagtggc tggaagaaaa ccgcgaacag
     1561 gtagagatga ttgaagaacg catcaaagcg cgttgcgttg gcgctgcgct gcgccagaac
     1621 ccgcaaatga agcgggcgct ggataatttt aaagcggtgc tggatttacg cgtcaaccag
     1681 agcgatatca ctgatgcaca aataaaaaag atcattgcgg tgatcgaccg cgccgctttt
     1741 gatattacgc aactggatta atcgccgcat ccgccagtgg cgcggtgcaa ttgccggatg
     1801 cgacgcttga cgcgccttat ccggcctaca cccgctacac accccgtagg cctgataaga
     1861 tgcgccagca tcgcatcagg cattgtgctc caaccgctgg atccggcata ccgattaatg
     1921 cagcaccgtc accgcgtctt ccagtcggct ggcgcggtgt ttcaccatcg ccgacacctg
     1981 cgcactctct tccaccaact cggcgttttt ctgggtgatc aggttaagct catccactgc
     2041 acgggtcagg ctggaaagcc catcggcctg ttccagcgtt gaatggctaa tctgagctat
     2101 caactgggtg acgtttttta cctgcgccac aatatcttcc atcgtccgtc cggcggcgtg
     2161 tacctgctgc gaaccagatt gcaccttatc agcactggca tcaatcagct tgcgaatatc
     2221 gttggcagca ttggcgctgc ggctggctaa atgacgcact tcccccgcca ccactgcaaa
     2281 acctttgccc tgttcgccgg cacgcgccgc ttccaccgcc gcattcaggg ccagaatatt
     2341 ggtctgaaac gcaatatcgt taatcagcga agtaatggtg ccaatgcgct gtgtactgtc
     2401 ggcgatatcg tccattgtct tgatcaccgt ggtcatcgcc tccccgccct gcactgccgc
     2461 attactggcg gtgattgaaa gtttatcggc agccgacgcc gtggcagagt tctgtttcac
     2521 cgacgccgcc atttggttca tggtcgccac cgtttgctga acgttatcaa ctgtctgctg
     2581 ggtatgttcg ttcagttcat cggtgccttt cgccagcgtc tcactgccat ttctgacact
     2641 ggacacctgg cttgagacat cgttaattag ccaacggcac atcaggccaa gctgccctac
     2701 cgcgcgtaat gtcagcccca gctcatcgct gcgattcaga tgctcaacac tattacgctc
     2761 tccggtcgcc accttcagcg cctgacgggc gacattttct atcgggcgga caatctgcca
     2821 ttcaaaacaa gcgcttgcca acaataccac taacgcacag aggatatacg tcaccaccgg
     2881 ggcagcaaca aaccaaagca tagccgccag caagataaac atcagggtca tcacgccacg
     2941 cgcccgccag cgaagcggta atgaaggcag tttacccagc cagcctttac gcaccaccag
     3001 gcctttatga atacgcttac cggtacgtcc ggcgttcagc gctttgtaca gcggctccac
     3061 cgccgcgatc tcttcatccg tcgcccgggt acgaatcgac atatagccac tgatttttcc
     3121 ctcgcgcacc atcggtaccg cattggcccg cacccaataa tggtcgccat ttttgcggcg
     3181 atttttcacg atgccgctcc agggttcccc ttttttcagg gtgaaccaca tatccgcaaa
     3241 cgccgctttt ggcatatccg ggtgacgcac catgttgtgc ggttgcccct gtaactcctg
     3301 caaggtatag ccgctcacct gcacaaaagt gtcattagca tgagtaatat agctttgcag
     3361 atcggtagtg gacatcagag tggtgtcgtc cgccagcggg gtattttgct gggtgacata
     3421 cggatgagaa gacatgttcg cgtcctgtgc aggttatatg gttgttaact tcttgtcaga
     3481 gtttatgtcg gccccgctgt ggttatcttt aaccaattaa tttgatttag atcgcaattt
     3541 acgattaaag cacaaatcta attccttgat ttaaaatact ttcactctgt tactatacga
     3601 aaacgttaat tatcttgccc aaaaatcagg caattattgc cctgaaagcg tgcatttgcg
     3661 cagcaatcat caaatccata cccgacaaaa accgtgcaaa ataacagcag atgttatcaa
     3721 atagcactaa atattgcgaa aatgataacc acatttgtgt ttatcccgat tttcgcgatc
     3781 gcagccagag tggcgcaatc cctgcaatac ttaaatcggt atcatgtgat acgcgagcct
     3841 ccggagcata ttttgaacag gttaccttcg agcgcatcgg ctttagcgtg cagcgcccac
     3901 gccctgaatc tcattgaaaa gcgaacgttg gatcatgagg agatgaaagc acttaaccgg
     3961 gaggtgattg aatacttcaa agagcatgtc aatccggggt ttttagagta tcgcaaatct
     4021 gttaccgctg gcggggatta cggagccgta gagtggcaag cggggggttt aaa
//
LOCUS       DAWDRC010000365         4066 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9141_length_4066_cov_2.471952, whole genome shotgun sequence.
ACCESSION   DAWDRC010000365 DAWDRC010000000
VERSION     DAWDRC010000365.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9141_length_4066_cov_2.471952"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          938 a         1095 c         1025 g         1008 t
ORIGIN      
        1 ggccgctttt cttgttgccg ccggtgacgc cgagtgtaga aaccagcgcc tcaccagggc
       61 cattcaccac gcagccgata atcgaaacgt ccatcggagt gatgatatct tccaggcgtt
      121 gctccagcgc gttaaccgta ccgataacat caaattcctg acgcgaacat gtcgggcagg
      181 cgatgaagtt gatcccgcgc gaacggatac gcagcgattt caaaatatcg aaaccgactt
      241 tgatctcttc gaccggatcg gccgccagcg atacgcgcag cgtgtcgccg atgccttcag
      301 acagcagcag acctaaacca atagcggatt ttactgcccc gctacgcgca ccaccggctt
      361 cggtgatccc cagatgcagc ggctgatcga tttgttttgc cagcaaacga taagactcaa
      421 cagcgaggaa gacgtcagac gctttcacgc tgactttgaa ctgatcgaag ttcaggcgat
      481 cgagatgatc aacatgacgc atagcagatt ccagcaacgc ctgcggcgtc ggttcgccat
      541 acttttcttg cagatctttt tccaacgatc cggcattaac gccaatacgg atcggaatgt
      601 ttttatcgcg cgcacagtca accaccatgc gaatacgctc ttcattaccg atattgccag
      661 ggttaatacg cagacaatcg acgccgtatt ccgctacttt cagcgcaatg cgatagtcga
      721 agtggatgtc agccaccagc ggcacgttaa cctgctgttt gatgagtttg aacgcttctg
      781 ccgcgtccat cgtcggtacg gagacacgga cgatatcagc gccaacacgt tccagcgcct
      841 tgatttgatt gaccgttgct tcgacgtctg tcgtacgcgt attggtcatg gactgtacgg
      901 cgatgggagc accatcgcca atcggcacat tcccaacgta aatacgtgtt gattttctac
      961 gttgaattgg agcctggtta tgcatgaaaa atctcccgcg ttacccgtct gttactgcgc
     1021 cggtgattgt tcggcattga gggtcagacg cgcaacctgg ttagttctga taaaacgact
     1081 cagatcgaca ggtttccctt gatactggat ctgtactgcg gctggcgcac caattttcag
     1141 tttgtacggt gcctggccgg ttaagttcaa attaccgtct ttacgctgca taccgctaaa
     1201 cagtttttta ccggtagcat cggtgacttc cagccagcaa tcggcagtaa agttcatcac
     1261 cagcgcattc gggtcaaccg ccggcgtggt cacgccagcc tgatcggttg gcaacggcgc
     1321 agcgccatct ggtgttgttg ttgccgctgg tgccggagtc gcagcggtat caacatttgc
     1381 ctgcgaaggt ggaacaaccg cattctgttg cggatcaaca gctggtgctg gcgcagttac
     1441 ggcaggtgtt tgcgtgttgg ttgcggtggt atccacaggt gatgtcggcg tggtcgccat
     1501 agcctgatct gtggttgtcg acgtatctaa cggtacactc tggctttgat tattattcag
     1561 ttccgcagaa gattgatcgg ccatagtggt gatctcttcc tgctgagctt tgtggtcttg
     1621 ccaccaccag gcaccgctca gaccaataac cacaaacaac accagccagg tgaaggtcat
     1681 cagccagccg tcacgttttt tgcggcgttt accgagggaa aaactctgca tcggcgcaac
     1741 ttttgcagcc cgtagtggag cctgcttttc cagccctggc agcagttctt cttctggaat
     1801 atgtaccaga cgcgcataag agcggatata tccgcgcagg aatgttgaag caagatcggc
     1861 aggtgcctta tcttcttcaa tgtcgcgtac cgtggaaacc ttcaggcaaa gtcgctcggc
     1921 aacggcctgt tgactaagtc ctagttgttc gcgagcatta cgcaggcgag cgccggtagt
     1981 aagtgcttca ttttggtcgt gcgtggcttc agtattcatt cgctgcaggt acgtttaaat
     2041 gagaattgag atgccggtga accatcatgc ccacccacac cgcgaaacat ccgttaagtt
     2101 aaccgttatc atacagtata agaccgtctg tgtgctcgtg acaaagccca cattcgctaa
     2161 ggctaactta ctgttgcatc gttacatact gccttaaagt cagcaaaaac gcaccgttaa
     2221 tattgaccag acaattgcaa cttaatactt cattcataaa agattacgcc acggtacata
     2281 aagtaaccgt ggcgtaatgg ctatcagacc gctttaatgt cgatggcttc accctgcatc
     2341 cgcttacgca gagtacgttt cgtacggtcg ataacatcgc ccgccaactg accacaagca
     2401 gcatcgatat catcaccacg agttttacgc acaatagtgg tgaaaccgta gctcatcagc
     2461 acttttgaga aacggtcgat acggctgttc gaactgcgtc catacggcgc gcccgggaac
     2521 gggttccacg ggatcaggtt gatcttacac ggcgtatctt tcagtagttc cgccagttgg
     2581 tgcgcgtgtt cagtgccgtc gttaacgtgg tcaagcatca cgtattcaat agtgactcgg
     2641 ccctgattgg cgttggattt ctccagataa cggcgcaccg ccgcaaggaa cgtttcgata
     2701 ttgtactttt tgttgatcgg cacaatctcg tcacgaattt cgtcgttcgg cgcgtgcagg
     2761 gaaattgcca gcgctacgtc gatcatatcg cccagtttat ccagcgccgg aaccacaccg
     2821 gaagtggaaa gcgtgacgcg acgttttgac aggccaaaac cgaaatcatc aagcatgatt
     2881 tccatcgccg gaacgacgtt gttcaggttg agcagcggct cgcccatacc catcatcact
     2941 acgttagtga ttgggcgctg accggtgact tttgctgcgc cgacaatttt cgccgcacgc
     3001 cacacctggc cgataatttc cgatacccgc aggttgcggt taaagccctg ctgggcggtg
     3061 gaacagaatt tacactccag cgcacacccc acctgcgaag agacgcagag cgtggcacgg
     3121 tcgtcttccg ggatatatac cgtttcaacg cgctgatcgc caacggcgat cgcccattta
     3181 atggtgccgt cagatgaacg ctgttcttca accacttccg gtgcacggat ttccgccacc
     3241 tctttcagtt tgccgcgcaa cactttattg atgtcggtca tctcatcaaa gttgtcgcag
     3301 caatagtgat acatccactt catcacctga tcggcgcgga agggtttttc acctaaatct
     3361 ttaaaaaact cccgcatctg ctgacggttg agatccagca ggttgatttt tccatctttc
     3421 gtggtgacgt tttcaggtgt gactaattgt tcagacatat gctattccgg cctcgttatt
     3481 acacgttatg gcccctggag ggttgaaaaa agaaacgccc cggtgagcgg ctgctcgtcc
     3541 gggggcgctg cattgtacaa attctggcgc acggatgcca cgtttgcacg cggcatttac
     3601 gaaattatta acgggtgcgc gggcacactt cgccttcgcc gaagaaataa gcgatttcgc
     3661 gagcggcaga ttcgacggaa tcagaaccgt gggtaccatt ttcggtcagg ctgtcagcgt
     3721 aatcagcacg cagagtaccg gccagtgcgt ttgccggatt ggtcgcgccc agcagatcgc
     3781 ggtgacgctg aacagcattt tcaccttcca gcacggaaac aacgatcggg ccagaagtca
     3841 tgaattcaac cagaccatca aagaacggtt ttccatcgtg ttcagcataa aagccacgag
     3901 cctgttcaac ggtcaggtgc agcattttgg tgccgacaat tttgaaccct gcagcttcaa
     3961 agcgcgcaaa gatactacca atcacgtttt ttgctaccgc gttcggtttg atgatggaaa
     4021 aagtacgttc aatagccatt tttacctctg taaattgttc tgttgt
//
LOCUS       DAWDRC010000366         4062 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9150_length_4062_cov_2.741452, whole genome shotgun sequence.
ACCESSION   DAWDRC010000366 DAWDRC010000000
VERSION     DAWDRC010000366.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9150_length_4062_cov_2.741452"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1085 a         1101 c          921 g          955 t
ORIGIN      
        1 gtatcgacag aatcaataat attataatta ccttcgctat tataaataat caatttttca
       61 cgagcgagtt gattcttagc aatgatctca tgaccttgct ttaaaaatag aacttcatca
      121 ttagcgctga tgtgatcaag atactcgttc agatatcgcc agtttaaatc gacatgaatt
      181 ttatctaccg ctaacaaatc atgatctttg ccgttgatgt gtagcgtaaa agagatacat
      241 tttttacccg gacgatcaaa gcaattgtat gccggattcc agctgttgtt agagtcatct
      301 gaagccatcg accacggtcg tttcgatggg ttaaaatcgg caaaaataac ccgccctgac
      361 tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa caacgatttt atcattcgcc
      421 ctccgcgaaa agagataata tttattatca tcaagtacca gtcccaccga gtctaaaaat
      481 ggcatcattt ccattttcag aatcacggca cgttgaatct cctctgtatt ttttaataaa
      541 tacagtgagt cctttaatgt cagggccgaa cgttcaatat ctttaaagaa atagccaacg
      601 tttttctgaa taagttcaaa gcggctatcg gcacgtttta ccgcgcgttc cgataccagt
      661 tgatagttaa tgatgaaaaa aagaccaata aatgacagtg ctgccaataa aaataaaatt
      721 atcagcctta tctttatctt ttcgtataaa ctcagcatgt taatacattt aacaaaataa
      781 ctatctgaac gctgtgccgc tgcaaaatag tcccttgctg cggcttttta accaaattac
      841 ccgattctac aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga
      901 ggtgattacc ctttcaagca atattcaaac gtaactatcc tttaattttc ggatccagcg
      961 catcgcgtaa accatcgccc aacaaattga acgccagtac ggtcagaaaa atagccaggg
     1021 ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca ttgagcattg
     1081 ccccccactc tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg ctggcggcag
     1141 agataatcga ggtgccaatg cgcatggtga aaaacaccac gatagaagag acggtcccag
     1201 gcaagatatg acgcaacaaa atggtcatat cgctggcacc aatactgcgt gctgactcga
     1261 taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac cagccgggca aacgcgggga
     1321 tggaaaaaat ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac accgcaacaa
     1381 cagcgatcgc cagtaaaata cccgggaagg caaacaacac atcgcaaatg cgcatgatca
     1441 gccgatccca ccagccttca taatatccag ccagcaagcc cagcaacgtc ccgatcgccg
     1501 caccgataaa cacggcaaac acgcccgccg ccagcgagat ttgcgcacca accaggacac
     1561 ggctgaaaat gtcgcgcccc agtgaatcga caccaaacca gtgctgcaaa gaaggtccgt
     1621 tattcagatt gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa
     1681 aaatggccac cacaatcaat aaaataacga acaatgcggc ggtcatcgcc atatgctgac
     1741 ggcgaaatcg tcgccagaat tcatgccacg gtgtacgtac ctggtcaggt ttgaccagtg
     1801 gcatggcgtt taacgccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct
     1861 gatagccggg ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc
     1921 cagcgagaaa agcagaattt ccgcctgaat cactgggtaa tcgcgcattt ctacggagtc
     1981 aacgagtaag cgtccaagtc cgggccagtt gaaaactttc tccaccacga tggaaccacc
     2041 gagcaagaag ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttgcgtag
     2101 cccgtgtttg aggacaaccc aggtttcgct cacccctttc gccctcgcgg tacgcatata
     2161 atcttcgctt aaaacgtcga caaacgacgc gcgggtaaag cgcgccatca cggcggccac
     2221 tgccgcgccg agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt
     2281 aggcagccag cccagttcaa cggagaatac ctgaattaaa agcatcccca gagcaaatgc
     2341 cggaaacgag atccccgaca ccgcaatagt catactcaat cgatccggcc aacggttacg
     2401 ccagacggcg gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt
     2461 tatggtcagc caaagcgttg gcataaagcg gctggcaatc tcatcggcaa ccggacgacg
     2521 cgacaccatc gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg
     2581 gtgatacagc ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc
     2641 agcttcgggc ccggcaatca atcgcgccgg atcgccgggc agcatgtgga caaataaaaa
     2701 caccagcacc gagacgataa acagcgtcgg aatcaacccc agtaagcgtt tgataacgta
     2761 attaagcatt ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg
     2821 gtgtctggca tgatccaaaa accggtcagg tttttactgt gtgccgacac cagtttttct
     2881 accaccagcg ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag
     2941 cgggtctttt ccgccggatc attagttttc aatgcctgag cgaggaagtc atccacctgt
     3001 ttattgctgt aaaacgcggt attaaacagc gtcggtggcc agttctgcga ggcaaacagc
     3061 ggcgatagag cccagtctgc ttcgccggtt gaagccgacc agccagtgta gaacatccgc
     3121 acaccgctct ctttttgccc tttaccttca acttctgccg cccgttgtcc ggcatccatc
     3181 gcagtcacct gggctttaat cccaacctgc gctaactgct gctgggtaaa ttgcagcact
     3241 ttctgcgcag tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc gttgggatat
     3301 cccgcctctt tcagtaattc gcgcgctttc gccggatcgt aaggccacgg tttataactt
     3361 tgcgcgtagg cgatactcgg cggtaccaca ccagtagctg gcgttgcata gcccgcgaag
     3421 gccactttca ccagcgccgg gcggttaatg gcgtaattca gcgcctcacg gaccttcggg
     3481 ttatcgaacg gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg
     3541 gccatcaact cgatattttt gtttttctcc agcagtgcgg cctgctcgta gggaatgggg
     3601 aaagcatact gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc
     3661 ggacgccagg ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt
     3721 ttcaccttca caaaatcggt ctgattccag gtatccagtt catacggccc ggttcccacc
     3781 ggatgaaaac caatctcctt gccatatttt tccagcgctg ccggtgaaat catcgcggtc
     3841 gccggatggg caagaatatt aataaacgct gagaatggct gtttgagggt aatctttacc
     3901 gttgtcggat cgatcgcttc cgttttggca atattcttat acaggttata gcgtttaaga
     3961 tgattcgccg gatcgctggc ccggtccaga ttcgctttca ccgccgctgc gttgaaatcg
     4021 gtgccatcct ggaatttaat gccttcccgc aatttcacgg tg
//
LOCUS       DAWDRC010000367         4051 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9178_length_4051_cov_2.159910, whole genome shotgun sequence.
ACCESSION   DAWDRC010000367 DAWDRC010000000
VERSION     DAWDRC010000367.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9178_length_4051_cov_2.159910"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    279..288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    571..580
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    965..974
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1098 a          928 c          855 g         1140 t
ORIGIN      
        1 gattatgggt tgtgaacgcc gggtcgttta cgatttcgtc aacgtcattc agggcgatgc
       61 aacgctaaac caggcgttaa ttaaagataa acgtactgaa aatctctata ttcttccggc
      121 atcgcaaact cgcgataaag atgccctcac tcgtgaaggg gtcgccaaag ttcttgatga
      181 tctgaaagcg atggattttg aatttatcgt ttgtgactcc ccggcaggga ttgaaaccgg
      241 tgcgttaatg gcactctatt ttgcagacga agccattann nnnnnnnngg ccgcgtaagc
      301 agaggtgaca tgctgagcat ggaagatgtg ctggagatcc tgcgcatcaa actcgtcggc
      361 gtgatcccag aggatcaatc agtattgcgc gcctctaacc agggtgaacc ggtcattctc
      421 gacattaacg ccgatgcggg taaagcctac gcagataccg tagaacgtct gttgggagaa
      481 gaacgtcctt tccgcttcat tgaagaagag aagaaaggct tcctcaaacg cttgttcgga
      541 ggataaatta tggcattact cgatttcttt nnnnnnnnnn ttcttgaggt catttgtaaa
      601 tatgtacaaa ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt
      661 tctattcttg agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt
      721 aaaagcgcat ttatcttcaa ggcagagtca tctctgcctt gagtttttca tccctctcat
      781 ccacgttgtg gtaaagcggc gagtattctt gctgatacta ttcattgcta tttccccctc
      841 cccctttaca ccttaaggct gttcccacac acagaacaaa atgtttacgc ctggttgagt
      901 aaataacctt attgttagcg tgggtatcct gggagatgat aaccccgcaa tttcattatt
      961 cattnnnnnn nnnngaggtt tcccgccgta actgacgggc ttttttttgc cccagaattt
     1021 tcccttttag cacccggtaa acaaaaacag cgccgaaacg cagtttaatc gttgatggtc
     1081 agaacgacca gttcacaccg gccaccgcat tccacgggga ctccacaccg gcaccacggc
     1141 tataccccac cccgagatgc ccgcttaacg tactgctgaa tgaggcttta atacctgcct
     1201 ggtatattcc acgtctgccc gacaaatcat tgacaaaatt accgtcacta ttcactttca
     1261 ctcgattatc atcgacaaat tctttgcgca cagccgcctt cagccagggt tcaatttcca
     1321 taccgttccc cagacgcatg ttgtaactca gcgttgcgcc cagttcacga tatatactgc
     1381 gggtatcgac tgatttcgat tccatgcatt ggataaatga tagtagcgtc taagtgacct
     1441 tagctctgct gcaacatcag cccgcaggaa ccagactagg ggattcatcc tgaagagaca
     1501 gcgcaaatgt attgtgttca ccgctcatca aagacatcat gatgaaatga tgatatcccg
     1561 tataagaaag aggtattttt taaacgcagt gcgctgaagt gttgttggat aaaaaatcac
     1621 tcttttctga taatactgtc gtacgcttat ttttcgacaa tgaggccgtc cacaaaataa
     1681 aatgactaat gtaatcgttg ctaatgaata tcttctgctc acataacaat cacacaataa
     1741 tattgataaa tcagattatc ttacctttac aacattgcgt taacagtcaa tgacacttct
     1801 tgaataaaaa tataatcaat gagttatttc tcatttgaga ttattctcat tcccgtaaaa
     1861 aacataattc ctatcatcac agtcacgctc attttgaatt atctgaataa tagcgagcgt
     1921 tctttagtta agcagatctt aattgaagac gaaattattg tgtgtgcaac ttacttaata
     1981 ccggacatct aagttgaaga aaaaaatact tgccttcggg cttatctcag cattgttctg
     2041 ctccacgcca gcgatggcgg atatgaatcg caccacgaaa ggtgcattgc taggcgcagg
     2101 cgttggttta cttactggca atggtgttaa tggcgtactg aaaggtgcag ctgttggcgc
     2161 tggtgttggt gcagtaacag aaaaaggccg cgacggtaaa aatgcgcgta aaggtgcaaa
     2221 agtgggtgcc gccgttggtg ctgtcactgg cgttctgacc ggcaatggcc tcgaaggggc
     2281 gattaaaggt gccgtgattg gcggtacggg tggtgcaatt ctgggtaaaa tgaaataagg
     2341 aatgatgatg aaaaaatttg ctttactggc gggtctgttt gttttcgcac caatgacctg
     2401 ggcgcaggac tacaatatta agaacggttt gccatcagaa acctatatta cttgtgcaga
     2461 agccaacgaa atggcaaaaa ctgacagcgc acaggttgca gaaattgttg cggtaatggg
     2521 taatgccagc gttgccagcc gtgatttaaa aattgagcaa tctccggaac tgagcgccaa
     2581 agtggtagaa aaactgaatc aggtttgcgc taaagatccg caaatgctgt tgatcacggc
     2641 aattgatgac accatgcgtg ctatcggcaa aaaataatat ttcatatgtt accgcgtcga
     2701 tgaattcgac gcggtttttt agctaatggc gggatcagaa cttatatttc aggcccacca
     2761 tcattgcgtt gtcattgtag ccattatcac ccagctgcac gcccacatta ccccagacgc
     2821 tggccgcagg attcagattg ccatttacgc catgtttaat ttcccccaga ttacgcgccc
     2881 cggcttgttc aactttcaca ccgtttattg agacagcaaa atctttactg ttatggatcc
     2941 cgttcatttc aacaaaaggt tcaaagtcgt caccgtgcgg accgctgttt ttctcctgag
     3001 tacgaataaa ggtgtggaaa ccaagtcggg tctggatatt gttatttgcg tcattctcaa
     3061 tacgtgagcc attggcttcc gtgtgcacat tatgatcgac gcccatccag atggcctgtg
     3121 ccgaggtcgt tctcaactgt ccgttgctgt cacgccaggc attatgcccg ccaatttgac
     3181 gtagccaaaa tcggcttgaa cgctcctgtt cagttacagg atcgatatag cgcgtttcac
     3241 ctgcacgatc gttcagacgc ataacaaaaa gagtgttagc ggctgccagg ttggcgacgt
     3301 aactgccagc ttcgggtcgc aggacggggg taggcgtttc tgttgattct gaagtgacat
     3361 tggaaaaaaa taactctcta ccattgtata tttttgcaat gggggcttta gtgccatttg
     3421 ttgtaataga acttttttat atcaatgtta ccttgtcgaa tagcaactgc ataacttcca
     3481 tcagccacag tttcaagcac cacataatct aaattaatat atgctttttt cccattagca
     3541 taagcaccat agctattaat accattagtt gttatggaac caccattcat ttttaaatca
     3601 gcacggctaa taacttcgta actgttagaa tcacaaactt catcatctac agcatcgaca
     3661 catgttcgag actctccagg acttgaacat gcaagaactc ctactgcatt tttccccata
     3721 ggtaacaatg ttcgttttat ctgtggtttc aagactggtg ttattttcca cagtcccaga
     3781 atcattaatc tgacttaaaa gtcccgctga atatgccccc tcgtctcgac tgaattatca
     3841 ccattcataa cgatggtgcc accaaattca ctgactactg cgctggctgt tatccctcca
     3901 ttttctttgt cagatacgct ttcagagcgt cttaatattg ctatcgctta tatataatac
     3961 tttgttttgg ctatcaacag aaataccata agatccatca ccactagtca tcacactggc
     4021 attatttata tatccagtta caccgtcatc c
//
LOCUS       DAWDRC010000368         4025 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9231_length_4025_cov_2.141814, whole genome shotgun sequence.
ACCESSION   DAWDRC010000368 DAWDRC010000000
VERSION     DAWDRC010000368.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9231_length_4025_cov_2.141814"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3805..3814
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1049 a         1035 c          958 g          973 t
ORIGIN      
        1 ctatggcaag gcggacccgc tggcattctg gctgagcgat caactggcgc gtaaactggg
       61 cggtcattta aacatcaaaa cgcgggatgg gcttggtaca cgctactctg tgcatatcaa
      121 aatgctcgca gctgacccgg aagttgaaga ggaagaagag cgtttactgg atgatgtctg
      181 cgtaatggtg gatgttactt cggcagaaat tcggaatatt gtcactcgcc agttagaaaa
      241 ttggggtgca acctgtatca cacccgatga aagattaatt agtcaagatt atgatatctt
      301 tttaacggat aatccgtcta atcttactgc ctctggcttg cttttaagcg atgatgagtc
      361 tggcgtacgg gaaattgggc ctggtcaatt gtgcgtcaac ttcaatatga gcaacgctat
      421 gcaggaagcg gtcttacaat taattgaagt gcaactggcg caggaagagg tgacagaatc
      481 gcctctgggc ggagatgaaa atgcgcaact ccatgccagc ggctattatg cgctctttgt
      541 agacacagta ccggatgatg ttaagaggct gtatactgaa gcagcaacca gtgactttgc
      601 tgcgttagcc caaacggctc atcgtcttaa aggcgtattt gccatgctaa atctggtacc
      661 cggcaagcag ttatgtgaaa cgctggaaca tctgattcgt gagaaggatg tttcaggaat
      721 agaaaaatac atcagcgaca ttgacagtta tgtcaagagc ttgctgtagc aaggtagcct
      781 attacatgaa caatatgaac gtaattattg ccgatgacca tccgatagtc ttgttcggta
      841 ttcgcaaatc acttgagcaa attgagtggg tgaatgttgt cggcgaattt gaagactcta
      901 cagcactgat caacaacctg ccgaaactgg atgcgcatgt gttgattacc gatctctcca
      961 tgcctggcga taagtacggc gatggaatta ccttaatcaa gtacatcaag cgccatttcc
     1021 caagcctgtc gatcatcgtt ctgaccatga acaacaaccc ggcgattctt agtgcggtat
     1081 tggatctgga tatcgaaggg atcgtgctga aacaaggtgc accgaccgat ctgccgaaag
     1141 ctctcgccgc gctgcagaaa gggaagaaat ttaccccgga aagcgtttct cgcctgttgg
     1201 aaaaaatcag tgctggtggt tacggtgaca agcgtctctc gccaaaagag agtgaagttc
     1261 tgcgcctgtt tgcggaaggc ttcctggtga ccgagatcgc taaaaagctg aaccgcagta
     1321 ttaaaaccat cagtagccag aagaaatctg cgatgatgaa gctgggtgtc gagaacgata
     1381 tcgctctgct gaattatctc tcttcagtga cgttaagtcc ggcagataaa gactaatcac
     1441 ctgtaggcca gataagacgc gttagtgtct tatctggcat ttgcactgaa tgccagatgc
     1501 ggcgtaaacg tcttatacag cctacgattc ccgcgatttc ctgaccctct cggcatatac
     1561 cgtcagcgtc tgttttatca catccagcgt taccggcttc gacaggcagc tgtccatacc
     1621 ggactccaga caccgttgct tctcttcagc caacgcatta gcagttactc cgattaccgg
     1681 caacgtcagt cccaactgac gaatgcgttg cgtcaagcgg taaccatcca tatttggcat
     1741 gttgacgtcg ctaagcacga tatcaatatg attcttgcta agtacattaa gcgcatcgac
     1801 gccatcattc gcggttttac attgatagcc caacgatccc aactgatctg ccagcagacg
     1861 ccggttaatc ggaagatcat ccacgcccag aatcatcata tcgtcgttat cgctgaccgc
     1921 tttgtccatc gacggcagag cgttagcagg atcgtcgctc tccatctcga tcaaataaat
     1981 acgcgccaac aatgccggta gctcatgcgg ggcagccaca ctgtgtaccc actcccctgg
     2041 cgctttctcc agcggaatac caatatggcg acgacagaag gtcactaccg ctctaccctg
     2101 ccatttttta ctcactacct catcagtaat taacacatct tcgggagtcg gttcctgccc
     2161 ttcgtatgtt gtaacgacga tgccgctgcg ctgcaaactg gtttccagga actgacagag
     2221 cgacgcattg cggaccgcca gccagcagcg tttaccactc aacccttcca cgcctttttt
     2281 ctgcgggtac tgagcgccgt acaacggaat acgcacggta aactggctgc ccattcccgg
     2341 ttctgaatct accgagatat cgccgtccat catgctgatc agtttttcac aaatcgccag
     2401 acccagaccg gtcccctgga agtttcgctg tacgcccgtt ccgacctgga agaagggatc
     2461 aaacaagcgc acgacttctt tcgccggaat ccccacgccg gtatcgcgaa cacggataga
     2521 gagataatcg ccatcagcgc gaacatgcag aactatacag ccggtatcgg tgaattttat
     2581 ggcgttactc aacagattgg agatgacctg ctgtaaacgc atcgggtcgc catttaaggc
     2641 cactggcaca tccggttcaa taaagcagta caagcctagc tgcttgcgta ccaccagcgg
     2701 taaatagttg gcggtgatgt ggttcatcac ttcacgcggt gaaaactcac gcggttcgat
     2761 cttcaactgt tctgattcaa tcttcgagaa atcgagaata tcgctgataa ttttcaacaa
     2821 caggctggaa gagttgttca ttgccgtcac cagacgatcg acgcctttcg gtaactcttt
     2881 ggtttgcaac agatcgaggt taccgataat gccatacagc ggcgttcgca gctcatgact
     2941 gactgtggca aggaacatcg attttgactg gctcgcctgt tccgctgctt gtgccatctc
     3001 ctgcaacgac tcttccatct tcacgcgcga agaaacatcc accagcacac aaatggccac
     3061 gttttcatta cgatagcgcg aatggacgaa gctgatttgc agattggtat tgttgctggt
     3121 caggacatca acaaaattga cctgctgccc acagataatt tgtgtcagcc gttggcgatc
     3181 ctcatgcgta agcatattga gataggtatg cgccagttcg ttacttaaaa tattgacgcc
     3241 atcagcggta cgcaaaatgc agatacccac tggcgcggag gcgacaatct tgcgattaaa
     3301 ctgctcatgt tcttccagtc gcagggcgtc gctttccgcc ggaatgaaaa tacgtcgctc
     3361 gtacatccgc gcgagagtaa acaatgcagc tccggcaagc acattcagca aaattgcgtt
     3421 aaggatcaac atgcgaatgc gttccagcac cttatcaacc ggcaccgaat acacgatgct
     3481 tagcgatgag ggtggcagat ttttcttcag caccagctcc cggaaccctt ccgtatagcc
     3541 aaaccaggag cgttcctgca tccagcgagg atcgccctta attttacttt ctggtccggt
     3601 aagcgaaatc agggtatgac cattttcatc aagaatggta acccccatcg gcaacgtacc
     3661 aggtaagaaa aaattctcca tccggatagt ctgctcgaca cccaaaagcg cctgcaaccg
     3721 gttcgccaga taaactggcg tcaacgcgta aaaatacccg acgccagggc gcggaccttc
     3781 gctgatccag tagaggttac tgccnnnnnn nnnntttcga tatttattaa tgcgttcatg
     3841 caaagctttt agcgcggtat cgcgttccac tggcatatca cgcagaccga aattggccat
     3901 gcagaggtta tcgctgccga ttaaaaatac ccggtttaga tcgtaagccg cagaaaaatt
     3961 atcgcgccag tagcgcataa accacgccaa tgactccaga gaacctcgcc aggtgttact
     4021 cattg
//
LOCUS       DAWDRC010000369         4016 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9248_length_4016_cov_2.450644, whole genome shotgun sequence.
ACCESSION   DAWDRC010000369 DAWDRC010000000
VERSION     DAWDRC010000369.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9248_length_4016_cov_2.450644"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          937 a          970 c         1036 g         1073 t
ORIGIN      
        1 cgtttctacc gtattaagct gcctgagtat cttggcttct ttgccggtaa acgctttgtg
       61 ccgatcattt caggcctggc tgccatcttt actggcgttg tgctgtcctt catttggccg
      121 cctattggtt ctgcaatcca gaccttctct cagtgggctg cttaccagaa cccggtagtt
      181 gcgtttggca tttacggttt catcgaacgt tgcctggtac cgtttggtct gcaccacatc
      241 tggaacgtac ctttccagat gcagattggt gaatacacca acgcagcagg tcaggttttc
      301 cacggcgaca ttccgcgtta tatggcgggt gacccgactg cgggtaaact gtctggtggc
      361 ttcctgttca aaatgtacgg tctgccagct gccgcaattg ctatctggca ctctgctaaa
      421 ccagaaaacc gcgcgaaagt gggcggtatt atgatctccg cggcgctgac ctcgttcctg
      481 accggtatca ccgagccgat cgagttctcc ttcatgttcg ttgcgccgat cctgtacatc
      541 atccacgcga ttctggcagg cctggcattc ccaatctgta ttcttctggg gatgcgtgac
      601 ggtacgtcgt tctcgcacgg tctgatcgac ttcatcgttc tgtctggtaa cagcagcaaa
      661 ctgtggctgt tcccgatcgt cggtatcggt tatgccattg tttactacac catcttccgc
      721 gtgctgatta aagcactgga tctgaaaacg ccgggtcgtg aagacgcgac tgaagacgca
      781 aaagcgacag gtaccagcga aatggcaccg gctctggttg ctgcatttgg tggtaaagaa
      841 aacattacta acctcgacgc atgtattacc cgtctgcgcg tcagcgttgc tgatgtgtct
      901 aaagtggatc aggctggcct gaagaaactg ggcgcagcgg gcgtagtggt tgctggttct
      961 ggtgttcagg cgattttcgg tactaaatcc gataacctga aaaccgagat ggatgagtac
     1021 atccgtaacc actaatccgt aagacgttgg ggagactaag gcagccagat ggctgccttt
     1081 tttacaggtg ttattcagaa ttgatacgtg ccggtaatgc tgaaattacg cggtgcgccg
     1141 tagacaatag aaccttccac gttggtatcg taggttttgt cgaacaggtt attgacgttc
     1201 ccctgtaacg agaagttttt cgtcacctgg tagcgggtga agagatccac cagcgcgtag
     1261 ctaccttgct cggcgcggaa ggtgccatac ggcgtcacgg tgtcggtata cacgcgattt
     1321 tgccagttaa caccaccgcc gaccgtcaac tctggcatga caggcaaccg atagctggtg
     1381 aacattttaa ccgtggtgcg tggcagatta ggattaacgg cgtttccttc gttatcctcg
     1441 gcaatatagc gcgttgcgcc aaacgtcaac tgccagttgt cggtaattgc gccgttgagt
     1501 tcaaattcca cacctttact gactgtccca tccaccgctt tataggcggt ttcgccgttg
     1561 ctgccgggga taggtgtacc ggtggactga gcgacattat cctgctcaat acggaagatg
     1621 gctaacgtgg tggtcagacg gctattcatc cagtccgatt tcagacctag ctcgtagttg
     1681 ttaccggtga ttggagtcag atatttgcct gaactgtcac gatcattttg cggctggaaa
     1741 atagaggtat agctggcgta ggtcgaccag ttgtcattga tgtcaaacac cagtccagca
     1801 taaggcgtgg tgtggttttt ctccatgctg taagtcagcg tatcaactcg ccagttggta
     1861 taacgtgcgc cgaggatcag atgcagcgga tcggcaaggg tgacacgagt ggcagcatat
     1921 aacgatttca tatgtgtggt atcgtcctgc gccaggctct gtggtgacca gtcggtttgt
     1981 gggaaattgc cattaaagtt gtagaaactg ccaatttcat ccgggaaaat gttggcccat
     2041 gaactgaagt aacgattgtt ttgtttgctg taactgccac caaacattaa attgtgctga
     2101 cgaccaaaca attcataact accgtcagcg aacagatcca gcgcatcaac tttacgtttg
     2161 ccgctgttcc aaccggtgcc gccgacataa tcaaagccag gtccataatt gctgtatggc
     2221 ccaaccagca taccatccgc tttgtttacg taggcatcga catacatcat tttgctgtca
     2281 aattcgactt cagagtgggt ggcattcagc gtcgcttgcc aggtatcagc aaaccgctgc
     2341 ttcagggtca taaagacctt gttgatctct ttatcgttgt acgcccagtc aggtgcggta
     2401 ctgcgtgcgc gatcgtaact gttgctgctg ccatcagtat tccagcgcgg taaaccaccc
     2461 caggttgggc tattaacatc aatacgctga tattcgtaac cggctgaaag catcgtaaga
     2521 tcgcctaaat cagcatcgac aatgccggag aagaaggtct tttcactgtt gtagcggtcc
     2581 agccatgagt cgttattctg gtagccgccg acaattcgtg cgcggatttt accgtcttcg
     2641 gtgagtgggc tttgtaaatc cgccacatac cgttctttgt tccagctacc gtattccgcc
     2701 gagacatcgc ctttaaattc acgactggtc gcgtgttttc gaaccatatt aattgccgca
     2761 gatggattac ccgtcccggt catgaggcct gtcgcgccac gcactacttc tacgcgttca
     2821 aacagtgcca tatcagaaag tgcgtcgccc agattccagc gcgattcaaa ataggtgggg
     2881 ataccatcaa ccatatagtt atcgatctgg aatccgcggg aataataaag agcacgatcg
     2941 gaatccgcct gacttttgct gatccccagc gtgttttcca tcacttcgcc cagcgtttgt
     3001 aactgctgat cttccatccg ctgctggcta acaatagtga ccgactgagg aatatcacgt
     3061 tgagtcatct gcatttttgt acccgcagag gtagacgtta cgttgtaatc attttcgcca
     3121 tcatctgggg ctgtggctga accctcaaca atcaccgttt cttcagtggc tggtgcagca
     3181 aaagcggcgg aaggtaatag tgccagtgct atgcaaccgg caaggagtga cggtttgatg
     3241 atggcttgat gttgattatc cctgttaaat tgtgtcgaaa gcatttgaaa tctcttggtt
     3301 tatgttggcg tcaggctcat gccggagcag ggtgatgagc ggttatattt agtgtgcgaa
     3361 taatttttgt gtgtaaatgc aaatgagaaa tatacgcatt catatttgga gtgtaaacca
     3421 aacagagaat gtcttttcag cgcattcgca ggcagaaatg ggaataatgg cgatatatac
     3481 ggcagcaaaa cgattttttg cttaagaaat cgtgagttaa ggttgaaaga gcaggtttaa
     3541 ctcgaccata ctctatactc gcagtgtggc gcggcgtagc atggcgcaac gcatggctat
     3601 ttgaaaaagg aaaatgtcgt ggcagaagaa actatattca gcaaaattat tcgtcgtgag
     3661 atcccctccg atatcgtcta ccaggatgat ctggtaacgg cgtttcgcga tatttcgccg
     3721 caagcgccaa cgcatattct gatcattccg aatatcctca ttccgactgt gaacgacgtg
     3781 tcagctgagc atgagcaggc gctgggacgc atgatcaccg tagcggcaaa aattgctgag
     3841 caagaaggta ttgccgaaga tggctatcgt ctgatcatga acaccaaccg ccatggcgga
     3901 caagaggttt accacatcca tatgcatttg ttgggtggct gtccgctggg accaatgctg
     3961 gcgcataaag gtctgtaacg atgagaaaag gatgctttga gctgatgact ctggcg
//
LOCUS       DAWDRC010000370         3989 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9308_length_3989_cov_2.417895, whole genome shotgun sequence.
ACCESSION   DAWDRC010000370 DAWDRC010000000
VERSION     DAWDRC010000370.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9308_length_3989_cov_2.417895"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1042..1051
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2589..2598
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1007 a         1100 c          926 g          936 t
ORIGIN      
        1 catccccgac gctaatcaca ataaatggct tgcctaaggt gatatttggc gcgtcgagtt
       61 cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa atcactgtgc cagacctgac
      121 cggtatagat gcctatcttg cgttcttcca cgttctgatt gccatcaacc atcaatgact
      181 caggtaacca aaaatgaata aaacttcttt tccgctccac ggttttatcg tcttcctgca
      241 ccgtataaag ctcggtcaca ggaatagcga tgagcggccc aacgagtttt tgcggcccgc
      301 tggtactttg gcgaatcaca tcttccacat cgatacggta atcggcacgt tcgacaatca
      361 cctgccgaat cagcattatc ggaatcaaca acagcaatac tgcaccaaac aaggtggtca
      421 ttttccagaa caggggggat ttcaacatag tcttctcctt tgcaatgaca gcaggatacg
      481 aaggctatgt gggaagaatt tgaagctatg tgaagtgacg gtgaagtcga agcgaggcca
      541 gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc gttaaacaaa cgggcgacct
      601 cactgacaaa cgccaacccc agaccgctgc ttttttgccc atttgcacgc ggcaaagagt
      661 aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat cccactaccg gtatccagca
      721 ccttaagggt gacgtattcc tgatccactt cggcgcttag cgttatgcaa ccgctctcgg
      781 gagtaaaatc gatggcgtta tccagtaaat tccccagcgc ctgctccagt aacgccggtt
      841 cagaagcaac gttaacctca gtaggcataa catgcaaagt gatgtttttt tctgccaact
      901 gcacggtgcg cgcttcgctg acgcggcgaa ataatgccgc cacatcaaca gcagtcagaa
      961 cgacttcctg acgattctcc agtcttgcct ggcgtagtaa cgtttccacc agtgcctgca
     1021 ttcgcgcatt ttgcgtcaga annnnnnnnn nggtaaaacg agccaccact tccggcggcg
     1081 gaccttcgcg taaaatttcc gccgcgccac gaatcgccgc cagtgggctt tttagctcat
     1141 gagttaacgc ataaacatac tgctcaatat agtttttccc ttccagcttc acgcgcatac
     1201 tttccagcgc ctgcgcgagt ttacgcaact cgctactacc gagatcgggg agaggaacgg
     1261 gcttattgtc agtgacggaa tcagcatagc gagtgagccg ggcaatagag cggttgatcc
     1321 accaaaccat gcctgcgcca atcaccagtg caatccccaa caaaatggcg ctggcccata
     1381 aaattcgccg ctcgctacgc ttaatgaccg gagccatcgc cgcgttcggt ttgcctacgc
     1441 tcaaaacgcc aataagccgc gagccgtcca taatcggcgc ggcaacatac atcacagaac
     1501 tttcgggatc ggcaggattt tgcaacgtgc tgcgcgcacc atactgacca cgcaacgtta
     1561 gccagacgtc attccagcgc gaataatcct gtccaacggc tttatttgcc gaatcgaaca
     1621 atactttgcc ctgcgcatcg gtcatataga catgatattc gttgcgcact ttgttaatgc
     1681 caccgatatt ggcgcgaaac gggcgatgtt gtagctgatt aaacgcctgc gccagttgcc
     1741 catgcgttgg gtccccagag agcaaatcgg gacgcgccag ctccgccagc aacgttgcgg
     1801 tgtcgattaa cgtcccctcg gttgctcttc gcacgcccgg tttaacttct ttgacaaaaa
     1861 tagccagaac gaaccaggcc gccaccgcca ccagtaaaaa atagcccagc aacaaccgca
     1921 tgccgatacg cattacaggc ccctcaggct atatcccatg ccgcgatgag tattaatcgg
     1981 tgaaagatcg gggttgatgg cgcgcagctt ggcacgcagc gttttaatgt gggtatcgac
     2041 ggtgcgatcg taggtgtcct gcgcatcttc ccacacgcta tccattagtt gctggcggga
     2101 ccagacgcgg cccggcgact tgagtaacgt cttcagcaat aaaaactcat accgcgtcag
     2161 cgttaatggc gtgtcaaacc agctgatctg cgccgcaggt tcattcaatt caaaatgacc
     2221 aatacggata acgggagacg gcgtcgagaa cttcttcacc cgacgcagta aggtgcgcac
     2281 tctggcgcac acttcgcgag gtgaaaacgg tttagccacg tagtcgtcag cgccaatttc
     2341 cagcccaagc aggcgatcga cctcttcact tcgggccgtc aggaacagta caggtaacgc
     2401 cggatggagc gccagtaact ggcggcacaa ttcaaagccg ctgatatcag gcaaaccaac
     2461 atcgagaatc atgacatcgg gaacctgctg gcgagcttta tccagcactg gcaagcctcg
     2521 ctcaaagacc tcgacgtcaa aaccttcctg ttgcaacatg tagaccagcg tatcggctat
     2581 cccttgctnn nnnnnnnntc ttcgactaac cagaccgttt cccgttgcat aaatcgcctc
     2641 tgttattgcc gccacggcat gacaggaacc gcgctaatcg cgtttttcgg cgaaccttct
     2701 acaactttgt cggagtaagc cagataagcg agcgcgttgc gtttagcgtc ataaaagcgc
     2761 acgacctgta acgacttaaa gaccagggac gtgcgttttt tgaatactac ctcgccctga
     2821 gctttgcagt ttttaatacg atccgatagt tcaatcggcc cgacttgttg acaagaaatg
     2881 gccgcatcgg aggtatcttc tgccaggccc aatccccctt taataccacc ggttttcgcc
     2941 cggctcacat aacaggtgac atttttcaca tcgggatcgt caaaggcttc aacaacaatt
     3001 ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg aaccaatctc ttctgcatga
     3061 gccaatggca ccagcattat caggcttaaa gacaggatta aatgcttgta tttcatatcg
     3121 ttaccatttt tatctattac acgagcaatt agttcgtcac ggtaaataac atattgatta
     3181 agaacaaaat ctcaatactt ttatttccgt caagacctaa aacatactcg actaagaaaa
     3241 aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca attacctgat gtcaggtgct
     3301 cgttgttgaa aggatgagga tattttatgg atcaggccgg cattattcgc gaccttttaa
     3361 tctggctgga aggtcatctg gatcatcccc tgtcgctcga caatgtagcg gcgaaagcag
     3421 gttattccaa gtggcactta cagagaatgt ttaaagatgt cactggccat gctattggcg
     3481 cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc actacgcctg actgcgcgtc
     3541 cgattctgga catcgcgctg caataccgct tcgactctca acagacattt acccgcgctt
     3601 tcaagaagca gtttgcccag actcctgcac tttaccgccg ttctcctgaa tggagcgcct
     3661 ttggtattcg cccgccgcta cgtctgggtg aattcactat gccagagcac aaatttgtca
     3721 ctctggaaga tacgccgctg attggtgtta cccagagcta ctcctgttcg ctggagcaaa
     3781 tctctgattt ccgccatgaa atgcgctacc agttctggca cgattttctc ggcaactcgc
     3841 caaccattcc accagtgctt tacggtttga acgaaacacg tccgagccag gataaagacg
     3901 acgaacaaga ggtgttctac accaccgcgt tagctcagga tcaggcagat ggctatgtac
     3961 tgacggggca tccggtgatg ctgcagggc
//
LOCUS       DAWDRC010000371         3973 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9356_length_3973_cov_2.189638, whole genome shotgun sequence.
ACCESSION   DAWDRC010000371 DAWDRC010000000
VERSION     DAWDRC010000371.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9356_length_3973_cov_2.189638"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    811..820
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          913 a         1018 c         1072 g          960 t
ORIGIN      
        1 tgttacgctg agtgaaagcg gtaataccat tagtggcatg acaggcgcgg atgggacggc
       61 aagtttttcg ctccgccagg ataatacccc tggttataaa acgccactga cggttactct
      121 ggctaattac gcttccgcga cagacacgct ggacgctatt tttacggtac caaccagccc
      181 aaatgtttcc tcggcgcact tttggggaca tatggcagac acggtagtgg tgaatagtaa
      241 atcgctacat cgaccgttgt taacgacgga attgccgtcc ggggctaatc ccgtatcctc
      301 accgattatt aactacgaaa actgggcttc tgcacatatc attgatgcca gtaaatggga
      361 tatcgcaagg cagtgtggca gcatagaaaa tgcaccgaca tacaatgaat tagagttgtt
      421 gcatactgtt tttaattcac tgggatggcc gtcttcaccc agttttcctt atttgtcctc
      481 tcaacagtgt gggatggacg aaggtactgg agcacaggat tgtagtatca cgctaataaa
      541 taaaccgggt ctggtgacct gcttccagta accagcatgg cggatctgga gtagcctggg
      601 agttattgcc ggatgcgatg ctggcgcctc ttatccggtc tacggattag acgttttttt
      661 acgtgatttg cgggtttttg ttacggcgct gacttcacta atcaccacac catctcccag
      721 tcgtgtgatc agtgtctcac ccactttcac ttgcttaacc tgttttaaca ccgcgccatc
      781 agcggcgctg gtgacgctat aaccgcgcgc nnnnnnnnnn tgggcttacg gcttcgaggt
      841 gtgttactgc attaccgaaa cgttcacgcg tggcgctaag ctgtgcgcgc agggtttctg
      901 ctaaacgata ttccagttgc tgaatgcgcg tttgcgcgcg atgaatacgc gactgcggat
      961 tttgctggac gagttgccgt gttaatcgct gctgttgctg acccgcgcgt ttaagctgat
     1021 tctccagcgc aaagctcatc cgcttttgca gacgttcgag catagtttgc tgacgtgcca
     1081 gccggagctg cggatgctgt tgctgcaagc gatgatgaat ctgcgtaaaa cgacgcgtgc
     1141 ggttggcgag ataataatcc atcgccattt ccagccgttg atgggtcgat tgcacctgac
     1201 gtagcaattc ttgctgattt cggctcacca cttcagcggc ggctgacggc gttggcgcac
     1261 gcagattagc aacaaagtcg gcaatcgtca catccgtctc atgcccgacg gcgctgacta
     1321 tcggaatgcg actggcaaaa atcgcccgcg ccacacgctc atcattaaaa ctccataaat
     1381 cttccagcga accaccgcca cggccaacga tcaacacgtc acactcgttg cgcttattcg
     1441 ccagttcaat ggcacgaacg atttgccccg gcgcatcatc cccctgtacg gcggtgggat
     1501 aaataatcac tggcagagaa ggatcgcgac gttttaacac atgcaaaata tcatgtagcg
     1561 cagcaccggt ttttgaggtg atcacaccaa cgcaatgcgc aggggagggg agtgattttt
     1621 tgtattgcag atcgaacaga ccttcagtct gcaacttcgc tttaagctgt tcgtacttct
     1681 gttgcagcaa tccttcaccg gccggctgca tactttcgac gataatttga taatcgccgc
     1741 gcggctcgta gagcgtaata ttggcgcgaa ctaaaacctg ttgcccgtgc tgtgggcgga
     1801 aggtgacccg gcggttgctg ttgcggaaca tcgcacagcg cacctgcgcg gtatcgtctt
     1861 taagcgtaaa gtaccagtga ccggaggctg gttgtgtaaa attagagatt tcgccgctga
     1921 tccacacctg ccccatctca tgctcaagca gcagacgaac cgtttgattc aggcggctaa
     1981 cggtaaaaat tgcaggggat tgagaaggta acatgtgagc gagatcaaat tctaaatcag
     2041 caggttattc agtcgatagt aacccgccct ccggggatag caagcatttt ttgcaaaaag
     2101 gggtagatgc aatcggttac gctctgtata atgccgcggc aatatttatt aaccactctg
     2161 gtcgagatat tgcccatgct acgtatcgct aaagaagctc tgacgtttga cgacgttctc
     2221 ctcgttcctg ctcattctac cgttctgccg aatactgctg acctcagcac tcagctgacg
     2281 aaaactattc gtctgaatat ccctatgctt tccgcagcaa tggataccgt aacggaagcg
     2341 cgcctggcta ttgctctggc tcaggaaggc ggtatcggct ttatccacaa aaacatgtcc
     2401 attgaacgcc aggcagaaga agttcgccgt gtgaaaaaac acgaatctgg cgtggtaacc
     2461 gatccgcaga ccgtactgcc aaccacgacg ctgcgtgaag tgaaagaact gaccgagcgt
     2521 aacggctttg ctggctaccc ggttgttacc gaagaaaacg aactggtcgg cattatcacc
     2581 ggtcgtgacg tgcgttttgt gaccgacttg agccagccgg ttagcgttta catgacgccg
     2641 aaagagcgtc tggtaaccgt gcgtgaaggc gaagcccgtg aagtggtgct ggcaaaaatg
     2701 cacgaaaaac gcgttgaaaa agcgctggtg gttgatgatg aattccacct gatcggcatg
     2761 atcactgtga aggacttcca gaaagcggaa cgtaaaccga acgcgtgtaa agacgaacaa
     2821 ggccgtctgc gtgtaggtgc agcggttggc gcgggcgctg gtaacgaaga gcgtgttgat
     2881 gcgctggttg ccgcaggcgt tgacgttctg ctgatcgact cctctcacgg tcactctgaa
     2941 ggtgttctgc agcgtatccg tgaaactcgc gctaaatatc ctgacctgca aatcatcggc
     3001 ggcaacgtgg caactgctgc aggtgcccgc gcgctggcag aagccggttg cagtgccgtt
     3061 aaagtgggta tcggccctgg ttctatctgt acgacgcgta tcgtgactgg tgtcggtgtt
     3121 ccgcagatca ccgccgttgc tgatgcagta gaagccctgg aaggtaccgg cattccggtt
     3181 atcgctgacg gcggtatccg tttctcaggc gatatcgcta aagctatcgc cgctggcgca
     3241 agcgcggtga tggtaggttc catgctggca ggtactgaag aatctccggg tgaaatcgaa
     3301 ctctaccagg gccgttctta caaatcttac cgtggtatgg gttccctggg cgcgatgtcc
     3361 aaaggttctt ctgaccgtta cttccagagc gacaacgctg ccgacaaact ggtgccggaa
     3421 ggtatcgaag gccgcgtagc ctataaaggt cgcctgaaag agatcattca ccagcagatg
     3481 ggcggcctgc gctcctgtat gggcctgacc ggctgtggta ctatcgacga actgcgtact
     3541 aaagcggagt ttgtacgtat cagcggtgcg ggtattcagg aaagccacgt tcacgacgtg
     3601 accattacta aagagtcccc gaactaccgt ctgggctcct gattctcttc gcccgacttc
     3661 atgtcgggcg atttatatta tctgtttcac ttgcctcgga ataagcgtca atgacggaaa
     3721 acattcataa gcatcgcatc ctcattctgg acttcggttc tcagtacact caactggttg
     3781 cgcgccgcgt gcgtgagctg ggtgtttact gcgaactgtg ggcgtgggat gtgacagaag
     3841 cacaaattcg tgacttcaat ccaagcggca ttattctttc cggcggcccg gaaagcacca
     3901 ccgaagaaaa cagcccgcgt gcgccgcagt atgtctttga agcaggcgta ccggtattcg
     3961 gcgtttgcta tgg
//
LOCUS       DAWDRC010000372         3944 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9424_length_3944_cov_2.815120, whole genome shotgun sequence.
ACCESSION   DAWDRC010000372 DAWDRC010000000
VERSION     DAWDRC010000372.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9424_length_3944_cov_2.815120"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2202..2211
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1027 a         1010 c         1020 g          877 t
ORIGIN      
        1 gtttaaccta tattgatatg tgctacgtat cttatttact tccgatttac taaagaaact
       61 gaatgtacct gtaaaaatta caggtttgga aagtagtgac atggcaaaat gattacagca
      121 gggactatga ggttaaaaac catatggaat gtcaaaaccg ttctgataaa tacatctggt
      181 ctccccatga cgcctacttc tataaaggac tatctgaact gattgtggat atcgacagat
      241 taatttatct atcgctggag aaaatcagaa aagatttcgt gtttatcaat ctcaatacgg
      301 attctttaac tgagtttata aaccgtgata atgagtggtt atccgcggta aaggggaaac
      361 aggtcgtatt gattgcggcc agaaagtcag aagccttagc aaattattgg tattacaaca
      421 gcaatattag gggcgtggta tacgctggac tgagtcgtga tattagaaaa gaactggcct
      481 atgtgattaa tggcaggttc ctgagaaaag atattaagaa agataaaatc actgaccggg
      541 aaatggaaat tatccgcatg acggctcagg gaatgctgcc taaatcgatt gccagaattg
      601 aaaattgtag tgtgaagaca gtgtataccc atcggcggaa tgcagaggcc aagctgtact
      661 caaaattata taagttggtt cagtaaactc caggcaagtt agttttaaaa aatgactcac
      721 cgggacttca tgtcctcaat tcaactcggg aagaaaagca atgaaaaaaa aggttctggc
      781 aatagctctg gtaacggtgt ttaccggtac aggtgtagcg caggctgctg acgtaacagc
      841 tcaggctgta gcgacctggt cagcaacagc caaaaaagac accaccagta agctggttgt
      901 gacgccactc ggtagcctgg cgttccagta tgccgaaggc attaaaggtt ttaactcaca
      961 aaaaggtcta tttgacgtgg ctatcgaggg tgactcaacg gctaccgcct ttaaactgac
     1021 ctcacgtctt atcaccaaca ccttaaccca gttggatacc tcaggttcca cactgaatgt
     1081 gggcgtggat tataacggcg cggcagtcga aaaaactggc gataccgtga tgatcgatac
     1141 cgccaacggc gtactgggcg gcaaccttag cccactggct aacggttaca atgccagcaa
     1201 tcgtaccacc gcacaggatg gtttcacttt ctccatcatc agcggcacca ccaatggtac
     1261 caccgcagta accgattaca gcactctacc ggaaggcatc tggagcggcg acgttagcgt
     1321 acagttcgac gctacctgga ccagttaatc tctctgatgt accagcaggg gtccccccct
     1381 gctttattcc tggacggaat tgttatgaaa aagcaccttc tgcctctcgc tctgctgttt
     1441 tccggaatat caccggccca ggcgctggat gtcggcgata tatcatcgtt tatgaacagt
     1501 gacagcagca cgctgagcaa aacgatccaa aacagtaccg acagtggccg cctcatcaat
     1561 atccgtctcg aacggctctc ttcaccgctt gacgacgggc aggttatcgc aatggacaag
     1621 ccggatgagt tgctactcac tcccgccagc ttgctgctac ccgcccaagc cagcgaagtg
     1681 atccgcttct tctataaggg accagcagat gaaaaagagc gctactaccg cattgtctgg
     1741 tttgatcagg ccctcagtga tgcgcagcgc gataatgcca accgtagcgc tgtggccact
     1801 gcttccgccc gcatcggcac cattctggtc gtcgcacccc gccaggcgaa ctaccacttt
     1861 cagtacgcca acggctccct gacaaataca ggaaatgcga cgctgcggat cctcgcctac
     1921 ggcccctgcc tgaaagccgc caatggtaag gagtgtaaag agaattacta cctgatgccg
     1981 ggcaagtcgc gtcgttttac ccgcgtggac acggcggata gcaaaggacg ggttgcactt
     2041 tggcagggtg ataagttcat tcccgtgaaa tagatagctg tgcagatgga taacgacaat
     2101 gcctttacga cggttctccc caggactgaa agcccagttt gccttcggca tggtcttttt
     2161 gttcgttcag cccgatgcca gcgctgctga cataagtgcg cnnnnnnnnn ngtggggtga
     2221 ttattccgca ggccttcagt caggcgcttc aggacggcat gagcgtcccg ctctatattc
     2281 atctcgccgg tagccagggt cgccaggacg atcagcgaat cggcagcgct tttatctggt
     2341 tggatgatgg acagttacgc atccgaaaaa tacagctgga agagagtgaa gataacgcca
     2401 gtgtcagcga acaaactcga cagcagctga cgactctggc caacgccccg ttcaatgagg
     2461 cccttaccat ccccctgact gacaacgcgc agttggatct cagcttgcgc caactgctgc
     2521 tgcagctggt ggtcaagcgc gaagcgctgg gcaccgtact acgctcacgt agcgaagaca
     2581 tcgggcagtc cagtgttaac accctcagca gtaatctgag ctataacttc ggcgtctata
     2641 acaaccagtt gcgtaacggc gggagcaaca cctccagcta tctgtcgctg aataacgtta
     2701 ctgcgctgcg cgaacatcac gtggtgctcg acggctcact gtacgggatt ggtagcggtc
     2761 aacaggacag tgaattatat aaagcgatgt atgaacgcga ttttgctggt caccgatttg
     2821 ccggtggaat gctcgacacc tggaacttgc agtccttagg gccgatgacc gccatttcag
     2881 cagggaagat ttacggcctt tcctggggaa accaggccag ctccaccatc ttcgacagca
     2941 gccaatcggc cacgccagtg atcgcctttt taccggcggc gggcgaagta catctcaccc
     3001 gtgatgggcg actactaagc gttcagaact tcaccatggg caatcatgaa gtggataccc
     3061 ggggtctacc gtacggtatt tacgatgtgg aagttgaggt aatcgttaac ggtcgcgtga
     3121 tcagcaaacg cacccagcgg gtcaataagc tgtttagccg gggacgtggc gtcggtgcac
     3181 cgctggcgtg gcagatatgg ggcggtagct ttcatatgga tcgctggtcg gaaaacggga
     3241 aaaagacgcg accagccaaa gagagttggc tggcaggtgc ctcgacctcc ggctcactga
     3301 gtacgtttag ctgggcggca acgggatatg gatacgataa tcaggcggtg ggtgaaaccc
     3361 gtctgacgct gccgcttggg ggggcgatca acgttaacct gcaaaacatg ctggccagtg
     3421 acagctcatg gagcaacatc gccagcatca gcgccactct acctggaggc ttcagttcgc
     3481 tgtgggttaa ccaggaaaaa acccgcattg gcaatcaatt gcgacgtagc gatgccgaca
     3541 accgtgcaat cggcggcaca ctcaacctga actcactgtg gtcgaagctg ggtacgttca
     3601 gcatcagcta caatgatgac cgccgctaca acagccatta ttacacggca gattactatc
     3661 aaagtgtcta cagcggtacc tttggttcgc ttggcctgcg ggccggtatt cagcgctata
     3721 acaacggcga cagcagcgcc aatacaggga aatatatcgc tctcgatctc tcgctaccac
     3781 tgggcaactg gtttagcgca gggatgaccc atcaaaacgg ctacaccatg gcaaacctgt
     3841 cagcacgcaa acagtttgat gaaggaacca ttcgcactgt tggtgccaat ctgtcacgag
     3901 ccatctccgg cgataccggt gatgacaaaa ccctcagcgg tggg
//
LOCUS       DAWDRC010000373         3943 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9426_length_3943_cov_2.414352, whole genome shotgun sequence.
ACCESSION   DAWDRC010000373 DAWDRC010000000
VERSION     DAWDRC010000373.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9426_length_3943_cov_2.414352"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1852..1861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2540..2549
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2945..2954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          901 a          983 c          993 g         1036 t
ORIGIN      
        1 ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg catgttgaaa tcgccgtgat
       61 ggtgatcttc gtcacttttt tccgcgtgat cgtgatcatc atcatcgtcg tgaatacttt
      121 tcatcagcag cggtttcaca tcttctagct gcgcaatcgt tacctgtttc gcttctggta
      181 atttacttac cggtttttgc atgaacgctt ccatctccgg gccaacccaa acgactaagt
      241 ccgcgttctg taagcgtttt acatccgatg gacgcagtga ataatcatgt tctgaagcgc
      301 cgtcaggcag taaaacctgc gtttctgtta ccccatcagc aatggcagaa gcgatgaacc
      361 caaccggttt aagcgaagcg acaacggcgg catctgcggc ctgtgttgca ccgccccaga
      421 gagcggcgga taatgctgcg aaaagaagcg tttttttatg taacataatg cgaccaatca
      481 tcgtaatgaa tatgagaagt gtgatattat aacatttcat gactactgca agactaaaat
      541 taacatgaca agtctggttt ccctggaaaa tgtctcggtt tcttttggcc aacgccgcgt
      601 cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt ttgactttac ttgggccaaa
      661 cggcgcaggt aagtcgacac tggtacgggt agtgctcgga ctggtaacac ccgatgaagg
      721 ggttatcaag cgcaacggaa aactgcgcat cggctatgta ccgcagaagc tgtatctcga
      781 caccacgttg ccactgaccg taaaccgttt tttacgctta cgccctggca cacataaaga
      841 agatattttg cctgcactga aacgtgtcca ggccgggcat ctgattaacg caccgatgca
      901 aaagctctcg ggtggcgaaa cgcagcgtgt actgttagcg cgagcattgt taaatcgacc
      961 gcaattatta gtgctggatg aacccactca gggcgtggat gtgaatggtc aggtggcgtt
     1021 atatgacctt attgaccaac tgcgtcgtga actggattgt ggcgttttaa tggtatctca
     1081 cgatctgcat ctggtaatgg caaaaaccga tgaagtgctt tgcctgaatc accacatttg
     1141 ttgttccggc acaccggaag ttgtttccct gcatccggag tttatttcta tgtttggtcc
     1201 tcgtggtgct gaacaactgg gtatctatcg ccatcatcat aatcatcgtc acgatttaca
     1261 gggacgaatt gttttgcgtc ggggaaatga tcgctcatga ttgaattatt atttcccggt
     1321 tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc tgggttcgtt tgtagtctgg
     1381 cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct cattacttgg cgtcgcgttt
     1441 ggtttgttgc tggacgtgaa tccattctat gcggtgattg cagttacgct gctgctggcg
     1501 ggcggtctgg tatggctgga gaagcgtcca cagctggcga tcgacacgtt attagggatt
     1561 atggcgcaca gtgccctgtc gctgggcctg gtggtcgtta gtctgatgtc taatattcgt
     1621 gttgatttga tggcttacct gttcggtgat ttactggcag tgacgccaga agatctcatc
     1681 tctattgcga ttggcgtggt catcgtggtg gctattttgt tctggcaatg gcgcaatttg
     1741 ctgtcgatga cgattagccc ggatctggcg tttgttgatg gtgtgaaatt acagcgcgtg
     1801 aaatttttgt ttatgctggt gaagtcattg cggagtggtg tagcgatgaa annnnnnnnn
     1861 nttattactt cactgctgat tattcctgct gctactgcac gtcgctttgc ccgcacgccg
     1921 gaacagatgg ctggtgtcgc tgttttggtg gggatggtgg cagtgactgg cggtttaacc
     1981 ttttccgcat tttacgatac acctgcaggc ccgtcggtgg tgctatgcgc ggcactgtta
     2041 tttattatca gtatgatgaa aaaacaggcc agctaatctt tcgctgaaca tatttgtcgg
     2101 atgcggcgcg agcgccttat ccgacctacg gttcgctatc tctggtaggc ctgataagac
     2161 gaacagcgtc gcatcaggca cactgccagt gtcggatgcg gcgcgagcga ccaatccgac
     2221 ttacggcatt tctggcggcg ttatgccaaa gtgattccac gcccgcgtcg tcgccatacg
     2281 cccacgcggt gtacgctgca aaaagccttg ctgaatcaaa taaggttcca gtacatcctc
     2341 aatggtttca cgttcttcgc caatggctgc cgccaggtta tccagaccta ccggcccacc
     2401 aaagaactta tcgattaccg ccagcaacaa tttgcggtcc atataatcga aaccttcagc
     2461 atcgacattc aacatatcca gcgcctgagc agcgatatct gccgagatgg tgccatcgtg
     2521 cttcacttcg gcgaaatcan nnnnnnnnnc ggttggcaat acgtggcgta ccgcgcgcac
     2581 gacgagcaac ttccagcgcg ccgtcatcac tcatctcaag ccccataaag tgtgcgctgc
     2641 gactgacgat atattgcaga tccggcacct gataaaactc cagacgttgc acaataccaa
     2701 aacgatcgcg caacggtgat gtcagcgaac ctgcgcgcgt ggttgcacca atcagggtaa
     2761 acggcggcaa atcaatttta atggagcgtg ccgccggacc ttcaccaatc atgatatcca
     2821 gttggtaatc ttccattgcc ggatacaaca cctcttccac cactggtgaa agacggtgga
     2881 tctcatcaat aaacagtaca tcgtgtggtt caaggttagt gagcattgct gccagatcgc
     2941 ccgcnnnnnn nnnngcgtag ttttacccaa ccccggagga ccaaaaatca acagacgatc
     3001 gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc tccatctgcg aacgaacctg
     3061 cggctgacca acatactctt ccagtaattt agggcggatg gcgcgatctg ccacatcttc
     3121 cggcaaagtg gtaccggcag aaatcagacg gtctgcttca atcatccttt acctcataac
     3181 gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg cgtcagggcg agcgattttg
     3241 ctcaccatgc ggcttgcttc ttgtggttta tagcccagtg ccaccagcgc agcaaccgct
     3301 tcctgttcag catcgtcggt cgccgggctg gcaggagacg tgagtaccag gtcggcggct
     3361 ggcgtaaaga gatcgccatg caaaccttta aatcggtctt tcatttcgac aatcaagcgt
     3421 tcggcggttt ttttgccaat acccggcagt ttcaccagtg cccccacttc ttcacgctca
     3481 acggcattaa cgaactgctg cgctgacatt ccggagagga tcgccagcgc caacttcggg
     3541 ccgacgccgt tggttttgat caactctttg aacaacgtgc gctcttgttt attgttaaaa
     3601 ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg tgaaaacgat cgcttcctga
     3661 cccgcttcag ggagttcata aaaacaggtc atcggcatat gcacttcata gcctacgccg
     3721 cccacttcaa ttaacaccag cgggggttgt ttttcaatga tgatgcctct gagtctgcct
     3781 atcacatgac gctcctgcgt aatgaatcaa agataatgct gtatgataaa aaaatgctgg
     3841 atagatatcc agcgaaggat gaagaaaact tgcgaggtgt ctcgatgatc tgaaaaatgg
     3901 cgcagtataa tttattctac aggttatatt ggaagcaaat att
//
LOCUS       DAWDRC010000374         3938 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9437_length_3938_cov_1.729333, whole genome shotgun sequence.
ACCESSION   DAWDRC010000374 DAWDRC010000000
VERSION     DAWDRC010000374.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9437_length_3938_cov_1.729333"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          927 a         1033 c         1158 g          820 t
ORIGIN      
        1 ttttacgcaa gggtgcgcgc tacgacattt ccatcgcact gcgctaaccg catttaatcc
       61 aggagaatca ttcatcgtgt tttggcgtga tatgacgttg tctatctggc gtaagaagac
      121 aactggcctc aaaacaaaaa agcgtttact gccgctgatg ctggcagcgg cattgtgtag
      181 ttcaccggtc tgggcggaag aagccacttt caccgctaat tttaaagata ccgacctgaa
      241 atcgttcatc gaaaccgtcg gcgctaacct taataaaacc atcattatgg ggccgggcgt
      301 tcaggggaaa gtgagtattc gcactatgac tccgctcaat gaacgccagt attaccagtt
      361 attccttaac ctgctggaag cacaggggta tgcggtcgta ccgatggaaa acgacgtgct
      421 gaaggtggtg aaatcaagcg ccgcgaaagt cgagccgctg ccgctggtcg gtgaaggcag
      481 cgacaactac gcgggcgatg aaatggtcac caaagtcgtg ccggtacgta atgtgtcggt
      541 acgcgagctg gcaccgattc tgcgccagat gattgacagc gcaggctcag gcaacgttgt
      601 taattacgat ccctccaacg tgattatgct taccggacgc gcctcagttg tggagcgcct
      661 gacggaagtg atccagcgcg tggatcacgc gggtaatcgc accgaagagg tgatcccgct
      721 ggataacgcc tcagcctcgg aaattgcccg cgtgctggaa agcctgacca aaaacagcgg
      781 cgagaaccag ccagcaacgc tgaaatctca aattgtcgcc gatgaacgca ccaacagcgt
      841 gattgtcagt ggtgaccccg ccacgcgaga caaaatgcgc cgtctgatcc gtcggctgga
      901 ttcagaaatg gagcgcagcg gcaacagcca ggtgttctat ctcaaataca gcaaagccga
      961 agatctggtc gatgtgctga agcaggtcag cggtacgctc acggcggcta aagaagaggc
     1021 ggaaggcacg gttggtagcg ggcgtgaggt tgtctccatc gccgccagca aacacagtaa
     1081 tgccctgatt gttactgcgc cgcaggacat tatgcagtcg ctgcaaagcg tgattgaaca
     1141 actggatatt cgccgtgctc aggtgcatgt cgaggcgtta atcgtggaag ttgccgaagg
     1201 cagcaatatc aacttcggcg tgcagtgggg gtcaaaagat gccggattaa tgcagtttgc
     1261 caacggtacg caaatcccta ttggtacgct gggggcagcg atttctgcag ccaagccgca
     1321 gaaaggttcc acggtgatca gcgaaaacgg tgctaccacc ataaatccgg atactaacgg
     1381 cgatctctcc acgctcgctc agcttctttc tggctttagc ggtacggcgg ttggcgtggt
     1441 gaaaggcgac tggatggcgc tggtacaggc ggtcaaaaac gactccagct cgaacgtgct
     1501 ctccacgccg agtatcacca cgctggacaa ccaggaagcc ttcttcatgg tgggccagga
     1561 cgttccggta ttaaccggct ctaccgttgg ctccaataac agcaatcctt tcaatacggt
     1621 agagaggaaa aaagtcggca tcatgctgaa agtcacgccg cagattaacg aaggtaacgc
     1681 ggtacagatg gtgattgagc aggaagtctc gaaagtggaa gggcagacca gcctcgatgt
     1741 cgtgtttggc gagcgcaaac tgaaaaccac cgtgctggct aacgatggtg agctgatcgt
     1801 gcttggcggt ctgatggacg accaggcggg agaaagcgtg gcgaaagtgc cgctgctggg
     1861 tgatatcccg ttgattggta acctgtttaa atcgacggcg gataaaaaag aaaaacgtaa
     1921 cctgatggtg tttatccgcc cgaccatttt gcgtgacggt atggcggcag acggcgtgtc
     1981 gcagcgcaaa tataactaca tgcgcgctga gcaaatctac cgcgatgagc aaggcttaag
     2041 cctgatgccg cacaccgcgc agccgatatt gccagcgcaa aatcaggcct taccgccgga
     2101 agtacgcgcg ttcctcaatg ccgggagaac gcgttaatgg tgcctgtagc acaggaaacc
     2161 accgctaaca ccgtgcgtct gccctacagt ttcagccgtc ggtttagcct ggtggcatgg
     2221 tgcgaagcgt cgctggagat cctccatgtt catccgttgt cgctctctgt tttgcaggag
     2281 ctacaacggg ggctgaacgc gccctttacg ctgcggcaaa tcgacgaggc cgaatttgaa
     2341 cagcggctga atgcggtctg gcagcgggac tcttccgaag ctcgccagct gatggaagat
     2401 ctcggttccg ccgaggactt ttttaccctc gctgaagaac tgccggaaac ggaagatctg
     2461 ctggaaagtg acgatgatgc gccgatcatc aaactgatca acgccatgct ggcagaggca
     2521 atcaaagaag gcgcttcgga tatccacatt gagacgtttg aaaagagtct ggtgatccgt
     2581 tttcgtgttg acggcacatt acatgaaatg ttgcgccccg gtcgtaaact ggcctcgctg
     2641 ctggtctcgc gtatcaaggt gatggcgcga ctggatattg ccgaaaagcg cgtaccgcag
     2701 gatggccgta ttgcgttgct gctgggcggt cgggcgattg acgtccgtgt atctaccatg
     2761 ccttccgcct ggggagaacg ggtggtgctg cgactgctgg acaaaaacca ggctcgcctg
     2821 acgctggagc gtctgggttt aagtctcgaa ctgactgcgc agttgcgcca gctgttacac
     2881 aaaccgcacg gcatttttct ggtgacgggg ccgaccggtt ccggcaaaag caccacgctg
     2941 tacgctggat tgcaggagct gaacaaccac tcgcgtaaca ttctcacggt tgaagaccct
     3001 atcgaataca tgattgaagg gatcggtcag acgcaggtta acacccgcgt cggcatgaca
     3061 ttcgcccgtg gcctgcgcgc aattttgcgt caggacccgg atgtggtgat ggtcggtgaa
     3121 atccgcgata ccgaaaccgc agaaatcgct gttcaggctt cactgaccgg acacctggta
     3181 ctttccacgc tgcataccaa cacagcggtg ggggcgatca cacgtttgca ggatatgggc
     3241 gtggagcctt tcctgctctc ttccagtctg acgggcgtga tggcgcagcg actggtccgc
     3301 acgctgtgct ccgactgccg tcaggccgcg cctgccaccg acgaagaaaa acgtcttatg
     3361 gggatctccg atacgcatgc cgtcacgctg taccatccgc agggctgccc tgcctgtaat
     3421 cacaaaggtt ttcgcggacg tactgccatc catgaactga ttgtggtgga cgccacattg
     3481 cgtgatttga tccaccgtca ggccggggaa ctggagctgg aacgttatgt ccggcaacac
     3541 tctgcgggta tccgcagtaa cggcattgag aaagtgctcg ccggagaaac ctctctcgat
     3601 gaagttctgc gggtaaccat ggaggcgtaa tggcactgtt ttactatcag gcgctggagc
     3661 gtaatggtcg caaaaccaaa ggcatgattg aggcggattc cgcgcgtcat gcccgtcaat
     3721 tgttacgcgg taaagacctc attcccgtgc acattgaagc ccggatgaat gcatcggcag
     3781 ggggaatgtt gcagcgtcgg cggcacgcac atcgtcgcgt ggcggcggca gatctggcgc
     3841 tgttcactcg tcaactggca acgctggtgc aggcagcaat gccgctggaa acctgcttac
     3901 aggcggtcag tgagcaaagc gaaaaactgc atgtaaaa
//
LOCUS       DAWDRC010000375         3934 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9445_length_3934_cov_2.105440, whole genome shotgun sequence.
ACCESSION   DAWDRC010000375 DAWDRC010000000
VERSION     DAWDRC010000375.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9445_length_3934_cov_2.105440"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    796..805
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1461..1470
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2185..2194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1011 a         1019 c         1081 g          793 t
ORIGIN      
        1 acaacggtac cgcgcgcttc tacggcacca ttgaagactc cccgtgctct atcgttccag
       61 acgatcacaa actggaagtc gatatgggtg acatcggtgc ggctaaactg accggcggtg
      121 gcactaccac accgaaagat ttccagatcc gtttacagga ttgcgtgttt accactgaaa
      181 caaacatgga gaccacgttc accggcacta actacgcggg gccagccaac acggataact
      241 atgcgttatt caatgttgac agcggtgttg ccatgaacca cgtgagcctg gtaatcggcg
      301 atacccatgg caaaggttat aaagtgggcg aaaagattgt tcagccaatc gtgatggata
      361 cttcaaccag caagggtaaa gacaagcaaa cgctgaactt taaagcctgg ctggtgggtg
      421 aaaccgatgc gccggatctc ggccagttcg aaaccctgac cacattccag attacctatc
      481 tctgatgtga caaccccaca gcgttctgct gtggggttat tcaacacgca acagatttac
      541 gactgtttca caaacggata tgtgtatgcc taatcactca aattttcggc tgcggggaat
      601 cgcctgctat attgcgctgg caatctctgg tgggtcagtc aatgcatggg ctgatgattc
      661 aattcaattt gacccccgtt tccttgagtt aaagggcgat acgaaaattg atctcggtaa
      721 gttttcaaaa aaagggtatg tcgacgcggg taaatataat ttacgtgtat ttataaataa
      781 acaacccctt tctgannnnn nnnnnaactg gtacgtctct gaaaacgatc caacaaaaaa
      841 ctatgcctgc ctgacacctg agttagtggc ggcgctgggg ctgaaagaag ggatagcaaa
      901 aagcctgcag tggacgcaca acgatgaatg ccttaaacca ggtcaattag acgggatgga
      961 agtcgagaat gatttaagcc agtcggcgtt gctgctgaca gtgccacagg cttacctcga
     1021 atataccagc agcgactggg accctccctc acgctgggac gacggtattc ctggcctgat
     1081 tgccgactac agcctcaatg cgcaaactcg ccatcaggag caggggggcg aggactcaca
     1141 tgatatcagc ggcaacggta ccgttggggc gaacctgggg gcatggcgtt tccgtgcaga
     1201 ctggcaaagt gattatcagc acacccgcag caacgatgac gacgatgaca gcagtaacag
     1261 tacaacgagc aaaaactggg actggagccg ttattacgct tggcgggcct taccctcttt
     1321 aaaagcgaag ctgtcgctgg gggaagatta tctcaattcc gatattttcg acggctttaa
     1381 ctatatcggc agcagtgtca gcaccgatga ccagatgctg ccgcccaatc tgcgcggcta
     1441 tgcgccagat gtttctggcg nnnnnnnnnn gggtacttta cgaaacccag gttcccgccg
     1501 ggccattccg cattcaggat atcggcgact ccgtctccgg cacactgcac gtccgcgttg
     1561 aagaacagaa tggtcaggtg caggaatatg acgttaccac cgcatctatg ccattcctca
     1621 cgcgccaggg gcaggtgcgt tacaaagtga tgatggggcg tccggaagac tggaaccaca
     1681 agaccgaagg cggctttttc tccggcggag aagcgtcatg gggggtggca gatggctggt
     1741 cgctttacgg tggtgcgctg gcagataaac actatcagtc ggcggcgatg ggggtggggc
     1801 gcgacctcgc acagtttggc gcgctggcgt tcgatgtgac tcactcgcac gtcaacctgg
     1861 atcatgacag cgcatacggc aaaggaaaac tggacggcaa ctcctttcgc gtgagctatg
     1921 ccaaagactt tgacgaactc aacagccgcg tcacctttgc aggctaccgt ttctccgaaa
     1981 agaacttcat gaccatgagc gagtatctgg acgcgaacca gtcggacatg gcgcggaccg
     2041 gtaacgacaa agagatgtat acgatcacct ataaccagaa ctttgccgct gcgggtgtct
     2101 ctatctatct caactactcc catcgtactt actgggatcg cccggaacag acaaactata
     2161 acctgatgtt ttcccactat tttannnnnn nnnngataag gggatgtacc tcagtatgag
     2221 cattccgtgg agcgacagca gcaccgtgac ctacaacggt tcctacggca gcggatcgga
     2281 cagcagccag gtcggttact ttaagcgcgt cgatgacgca acgcactacc aggttaacgt
     2341 tggcaccagc gagcagcacg gcagcgtgga tggttatctg agccacgacg gttcgctggc
     2401 gaaggttgat ctcagcgcca actaccatga aggggaatac cgttcggcgg ggatcgcctt
     2461 acagggcggg gcaacgctga ccgcgcatgg tggggcgctg caccgtaccc agaacatggg
     2521 cggcacgcgc ctgctgattg acgccgacgg gattgccaac gtgccggtcg aaagcaacgg
     2581 cgcgccggtg tacaccaata tgtttggcaa ggcggtggtc gccgatatta acaactacta
     2641 tcgcaatcag gcgtatatcg atttaaacaa cttgccggaa gatgcggaag ccacccagtc
     2701 ggtggtgcag gcgactctga cggaaggcgc aatcggctat cgtaaattca aagtgatcag
     2761 cgggcaaaaa gcgatggcgg tactgcggtt acgtgacggt agctacccgc cgtttggcgc
     2821 ggaagtgaaa aacgacgagc agcagcaggt tggcattgtg gatgatgaag gcaatgtcta
     2881 tctggcgggg gtcaatgcgg acgagcatat gatggtgttc tgggaaggca gcgcacaatg
     2941 cgagatcgta ttgccgaagc cgctacctgc cgatttgttc agcggcctgt tgttgccgtg
     3001 tgaacaaaag ggaacggcag cccctgattc ttcagcgcca gaaattaagc ctgttattca
     3061 ggaccagacg cggcaagtca caccaacgga agcgccgacg tcaatttcag caactcaata
     3121 atctgattaa ggaacgatca atgtcttata aattatcctg tccaatgctc gtcagcacag
     3181 cgctgatggc aatgcttacc accgccagcc tgacggcgca tgcatcggtc accccggatc
     3241 gcacccgtct ggtgtttaac gaaagcgata aatcgatcag cgtgacgttg cgtaacaaca
     3301 acgaaaagct gccttacctg gcgcaaagct ggctggaaga cgagaagggc aacaaaatca
     3361 cttcgccgct ggcagtactg ccgccggtgc agcgtattga cgctatgatg aacggccagg
     3421 tgaagattca ggcgctgccg gacattcaca cactgctgtc cgaccgagaa agcctgttct
     3481 actacaacgt gcgtgagatc ccgccaaagt ccgggaaagc caacaccctg caaattgcgc
     3541 tgcaaacgcg catcaagctg ttctggcgac caaaggcgct ggagaagatt gacatgagaa
     3601 gaccgtggca gtttaaggtc acactcacgc gcagtggcca ggactatacc gtcaataacc
     3661 cgacgccgta ccacgtcatc atcagcgatg ccagcacaca gaagaaaggg ctgactgccg
     3721 ccgggtttaa accgctggtg atgccaccga aaacgtcaca accgttaaag gcaaaattgg
     3781 cgagcgcccc ggtgctgacc tatatcaacg actacggcgc gcgcatgccg ctgatcttcc
     3841 gttgtgaagg gaacacctgc aaggtggatg aagatcaaag cagcaaaggt tgataacggg
     3901 agaagcaaca tgaaagcgtt ctttctgacc ggat
//
LOCUS       DAWDRC010000376         3915 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9486_length_3915_cov_2.101554, whole genome shotgun sequence.
ACCESSION   DAWDRC010000376 DAWDRC010000000
VERSION     DAWDRC010000376.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9486_length_3915_cov_2.101554"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1491..1500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2857..2866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          930 a         1033 c          942 g          990 t
ORIGIN      
        1 atgaacgccg ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat ctgccagaag
       61 cacgcgccag ccggatgtgt tcatcgccgg aaactgggtt atcactggaa acggcagcta
      121 aactaagtga agtcatgcca ggagctggcg gacgttcctg cctggaacgc tacgaatatg
      181 ccaaacacgg tgcctgcttt ggttttgatc cagacgcata cttcggcacg atggtacgcc
      241 tgaatcaaga aattaaagag agcgaagccg gaaaattcct tgcggataac tacggtaaca
      301 cagtgagccg ccgtgacttt gatgccgcct ttgccaaaag ctggggaaaa gagaacgtga
      361 aagcagttaa gctaacgtgc cagggtaacc ctgcgtattt aactgaaatt cagatctcga
      421 tcaaagctga cgccatcaac gctccgcttt ctgccaactc attcttgcca caaccacacc
      481 caggcaactg tggcaaaacc tttgtgattg ataaagcagg ttattaatcc tctgatttac
      541 aaaacacgct aattcgtctt ccataaacca tgacgattaa gatttataca taatgcgcta
      601 cattgaggaa ttccctgaat gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat
      661 catcatgtga gttaaaccct cgccgcctga cggtgagggt tttcttttgg gtatgtttat
      721 ctgcgacact cgcagtaccg acgacatgga gtaaaaatgt ccagaccaac tatcatcatt
      781 aacgacctgg atgccgaacg catcgatatt ctgctggagc aacccgccta tgctggtttg
      841 ccaatcgccg acgcgttaaa cgcagagttg gatcgcgccc aaatgtgttc gccagaagag
      901 atgccacacg acgtggtgac aatgaacagc cgggttaaat tccgcaatct tagcgatggc
      961 gaagtgcgtg tgcgcacgct ggtgtatccg gcaaaaatga ccgacagcaa tactcagctt
     1021 tccgttatgg ctccggtagg tgccgcactg ctggggctgc gcgttggcga ttccattcac
     1081 tgggaacttc cgggcggcgt tgcaacccac cttgaagtgc tggaactcga ataccagcca
     1141 gaagctgctg gcgactacct gctttaatcc catctgcaca agcgataact cgctttgccc
     1201 agaggatgca ctcgcatcct ctttccgctc aaatatcaac cagatttatc attttgcgct
     1261 taagcgcagc attctaatct gaactggtta ttcatctgcg gaacctcata tgagcgaagc
     1321 tctcggtatc actgcatttt atatattcat agccgcaatt attgttgctg cagtgctata
     1381 ccttgaacaa cgctggtagt gcctggtttt atttattttc cagctcaata gcaaattcag
     1441 atcaaatcag tctcgcaaat catagattca taaccaataa tgtgctgtta nnnnnnnnnn
     1501 catgcaaaaa caaggagaga atgcttatgt acaaaacaat catcatgcca gtggatgttt
     1561 tcgaaatgga attgagcgac aaagcggttc gccacgctga attcctcgcc caggatgacg
     1621 gagttattca tctacttcac gtactaccgg ggtcagccag cctgagcctg caccgttttg
     1681 ccgctgatgt acgtcgtttt gaagagcatc tgcaacatga agcagaagaa cgtttacaaa
     1741 cgatggtcag ccacttcacc atcgatcctt cccgcattaa gcaacatgtc cgttttggta
     1801 gtgtgcggga tgaagttaac gagttagcga aagaacttga tgcagatgta gtagtgattg
     1861 gttcgcgtaa cccatcaatt tcaactcatc tgttaggttc taacgcctcg agcgtaatcc
     1921 gccacgccaa tttgccggtg ctggttgtgc gttaattgcc agaataagtc tcccgccctg
     1981 cccggagcca tccctgcagg gctttttaat gcaataaaaa agccgccagg tttgaccccg
     2041 gcggctaagc aattacaggt gaatcttact tcttcttatg cagttttggt gcgaatcagg
     2101 tagtcaaaag cactcagaga ggctttggca ccttcgccag tggcgatgat gatctgcttg
     2161 tacggaaccg tcgtacagtc acccgctgcg aacacgcctt tgacgttggt ttcgcatttc
     2221 gcatcaatga taatctcgcc cctgcgatta cgttcgactg cgccttccag ccagttggtg
     2281 ttcggcagca gaccaatctg gacgaaaata ccggccagtt cgatgttgtg aatatcgccg
     2341 ctgacacgat cgcgatattc cagacctacg actttgctac cgtcgccttt cacttccgtg
     2401 gtttgcgcat tcagaataat gtcgacgttt ttcaggctgc gtagtttgtc ctgcagaacc
     2461 tggtcggctt tcatttctgg cgcaaattcc agcagcgtta cgtgctcaac gatacccgcc
     2521 aggtcaattg ccgcttccac gccggagtta ccgccgccga taaccgctac gcgtttgcct
     2581 ttaaacagcg ggccgtcgca gtgcgggcag taggtcacgc ctttggtgcg atactgatct
     2641 tcgccaggaa cgttcatgtt gcgccatttt gcaccagtcg ccacgataat gctgcgtgct
     2701 ttcagtaccg cgccagaagc tgtttcaatc tgatgcaggc cgccttcaac cgctgccggg
     2761 atcagtttgc tcgcactctg gctgtcgatc acatcaacgt cgtattcatc aacatgaact
     2821 ttcagcgcac ctgccagttt ctggccttcg gtcttcnnnn nnnnnnaacg ttcgcccatc
     2881 agaccggtac ggatgccttt acgtgcggag taaattgctg ccgctgcacc cgccgggccg
     2941 gaaccaacga ttaatacgtc ataagcatca cgcttgttca gctcttctgc cgcacgtttt
     3001 tccgcaccag tatcaatttt ggcaacgatt tcagtcaacg tcatgcggcc ctgaccaaac
     3061 tctttcccgt ttacgaacac tgccggaacg cccatcacgt tgcgatcggt gatttcgttc
     3121 tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg ggttcagtac gctcatcagg
     3181 ttcagcgcct gcaccacgtc agggcagttg tggcaagaga gcgagtaata ggtttcgaat
     3241 tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag actgcgcttc tttcgacgga
     3301 tgaccaccag tccacaacaa cgccagtacc agcgaggtga actcgtggcc cagcggggag
     3361 cctgcaaaac gtggtccctg gttggaacct gggttggtga tcaggaaaga cggcttacgc
     3421 accggcaagc tgttatcttc tttaaaggtg actttgtctg acagttctgc gatttcagcc
     3481 aacagttcct tgatttctgc cgatttagcg ctgtcatcca gcgtggcaat taactcaaca
     3541 ggcttggtca atttctcaag gtaagccttg agttgagttt tcatatttgt gtcgagcatg
     3601 tttatctcct gggctttaaa catcatcatg caagcggcct ctgcggctac ctggatgcag
     3661 cttgcatcat ggtgcgggga agaaaatcgg gtgccgcagc acccgattaa ttacgcgatt
     3721 gcggaactta gattttacca accaggtcca gagacggagc cagagttgct tcaccttctt
     3781 tccatttagc cgggcaaact tcacctgggt gagaagctac gtactgtgct gctttgattt
     3841 tacgcagcag gtcagacgcg tcacggccaa tgccttcagc ggtaacttcg attgcctgga
     3901 tgataccctg cgggt
//
LOCUS       DAWDRC010000377         3903 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9521_length_3903_cov_2.213877, whole genome shotgun sequence.
ACCESSION   DAWDRC010000377 DAWDRC010000000
VERSION     DAWDRC010000377.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9521_length_3903_cov_2.213877"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    328..337
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1731..1740
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2031..2040
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          987 a          881 c          926 g         1079 t
ORIGIN      
        1 tgggggaatt tgaagagtgt cttaaaatcc tcgacgcggt agattttccg ccagaacgca
       61 ttttgaatgt ttctccgcgc cgcttactga acttccttga atctcgcggt atggcaccga
      121 ttgcggaatt tgcagacctt taattactag taatggaaat atattaatga acgagttttc
      181 tatcctctgt cgtgtgctgg gttcgctcta ttaccgccaa ccgcaagatc ctttactggt
      241 gccgctgttt accctgattc gtgaggggaa actggctgcg aactggccac tggagcagga
      301 tgagttactg acgcgtttac agaaaagnnn nnnnnnngat tacaatgcgt tgtttatcgg
      361 cgatgaatgt gctgtgccgc catatcgtag cgcatgggtt gagggcgcga cggaagcgga
      421 agtgcgcgct tttctttccg aacgggggat gccattagcg gatacgccag ccgatcacat
      481 cggcacattg ctgctcgcgg cttcctggct ggaagatcag tcaacggaag atgagagcga
      541 agcactggaa acactgttca gtgagtatct gttaccctgg tgtggtgagt tccttggcaa
      601 agtggaggcc catgcaacta cgcctttctg gcgcaccatg gcaccgctaa cccgcgatgc
      661 tattagtgca atgtgggacg agctggaaga agattctgaa gagtaaatgt gatctgcatc
      721 actcataact gcaacagata atttgttgtt gcataaaatg tgtgctcgat ctcattcatg
      781 gccgcgtttt ctgctatcat gcgcggcatg aacatacttc tctctattgc aatcacaaca
      841 ggcattctct ccggtatctg gggatgggtg gctgtttccc tgggcttact aagctgggcg
      901 ggcttcctgg gctgtacggc ctactttgcc tgcccgcaag gtgggctgaa agggctggcg
      961 atctccgctg caaccctgct tagtggcgtg gtgtgggcga tggtcattat ttacggtagt
     1021 gcactggcac cacatctgga aattctcggt tatgtcataa ccggtattgt cgcgtttctg
     1081 atgtgtattc aggccaaaca gctgctgctt tcatttgttc cgggaacgtt tataggcgca
     1141 tgcgcgacgt ttgccgggca gggtgactgg aaactggtgt taccttcgct ggcgctcggg
     1201 ttgatatttg gttacgcaat gaaaaacagc ggcctgtggc tggcggcgcg tagtacgaag
     1261 accgctcacc gtgagcagca aatcaaaaat aaagcgtgag gggcactcac gctttcgctt
     1321 aaacagtaaa atgccggatt attaattccg gcatttttat ctatcaggat tccggtggaa
     1381 ccgacatatg gcggtatttc accagaatgt cattctgccg ttctgctttg ttctgcaaat
     1441 cccacagacc acggtcaata ccatcattaa tcaggaaaat gacccctgtt tcgatagccg
     1501 acatcaggca cagcataacg ggttcgttcg aggtgtaacc cacttcccct tccagcaagc
     1561 gctggtagtc aataaagcgg aaaaccccgg cctgaacttc ataggaaagt atcgtcttac
     1621 tggtgttcac cgaggagagg atctcaccgg tactcacatt gacgacacgc aggttcacgg
     1681 caatctgatc aagctggtat tgcgtgtctg caccgatccc aaaatatctt nnnnnnnnnn
     1741 gcccgattta acgttgcttt cataaccgat aattgaacct tcaaccatga tatttgccgc
     1801 cgttaaagat tgcagcggga ttcggttatt aatggcaact gtgccgtttt cttgtgccgc
     1861 acgaataatc ttgcgctcgt taagcaggtt ttgtaagccc tggcgctcca gcggtataaa
     1921 ccagcgagaa tctttcaatg ccgtgaccag cattgccgtg gcgctttgcg gaacagcagt
     1981 ggagaagtta cttgccgggt agggtttaaa ttgcccggtt tcgtcctgaa nnnnnnnnnn
     2041 ccgaaacaaa gatcttaccc gtcggcgctg gcagatgggt caaatctttg tagctctgag
     2101 cacgaggcat taatgtcggt ctggcggctt ctttaggcgg ggcggttaag catccgctca
     2161 gtaacatgac ggcaaccaaa agaaataagc gctgcatgat tattttcctt atgaagctgg
     2221 ggcttaaaaa tcggttgagt tattttgtaa acccgaaacc tggatggtcg aggtttgtcc
     2281 ggttttacga tctgtcacgt tcaactgcaa ttgaccatcg cggttagcaa tatcaacaat
     2341 ataatcgttg gtcaccatgc ggcccggttt accggtatta atattcgaca gtagcccacc
     2401 caaaatttgt gactggatgg cctgagtaaa gttatctaac gccgagggcg tttcaatacc
     2461 aaagtcatcg ttatagctcg gatctttata agagttttgg gcctgagcgc tatttaacaa
     2521 aaaagcgcca ttatttgggt taccaccaaa gtttggatta cggaactgga aagtcatggt
     2581 tccagcccaa cttaatggcg aaataagcat gagtagaact actgcatgtt tgacacgcat
     2641 tgcagcctcc gaacaatttt ttatttaaaa ttcatcatgc gccaaatcgc ccgtacttaa
     2701 taacgcctgg tttatctggc gacgatttag tgcttcttca gtttgaatca gtgcaaagac
     2761 gacagttttc tcgaagtctc ttttcaacgg aaataaaaaa gtctggaaaa taacgtcctg
     2821 attcaccgtt attgtgatcc agcttcccca tcgtgcactg ggcctttcat taatcgttaa
     2881 gttacccgta tagtcacttt cccatttatc actaaaggct cggtaaaaat catggccaat
     2941 agatgaaaca gtatggtcag ttaacaatcc cgggacttct acctcaacgg cgtgaagatt
     3001 ccctgcggcg aacagaaatt ctgccgccac aatccagcgt aaataacgtt tcatggcttt
     3061 atcgcctgag gttatcgttt gcccaggaaa ccgcttgtgt ccggtttttt acggctatct
     3121 tcttgaaaag attataaaga tgcgttttaa ccgtattttc gctgatgaac aacgaacgag
     3181 cgatctcgtt attagacgcg ccgatacgca gcttattcag gatctctttt tcccgatgag
     3241 taaggagggc tgattccgtg ctgttataac gatagttacc tgaatgcgta atcaggtaac
     3301 tggcaagctt ttgcgtaaag tagcattcgc cgcgcaggac gccttgcaac ccattgacaa
     3361 cacgttcttg atcctccatg gcataaaaaa cgccgttgat atgaggccag ttttcaatgt
     3421 cgcggtacgg gtaatcttca ggcgtattta gcaacaatat tttgatattg ttgtttttcc
     3481 tgctcaaagt atcctgccaa taatggataa gctttttatc cgcttccatc atatccagaa
     3541 gaataataga gcctgaagat atatcgtcca gagaacgttg aatattatgt aattttcctg
     3601 taattgccag cgattgttta aggtgctgca agagagctgt cgcctgcaaa gaaggtttag
     3661 tgatcaacaa taatgtatga ccatgaatac tatggacttc attaaacatg atgaaacccc
     3721 gcttttttta ttgatcgcac acctgacagc tgcctctaaa atagaagcac cagaagtact
     3781 gacagatgtt gcactgctgt gtgtagtaat aaatcagccc taaatgggta aaatataaaa
     3841 ctaatggatt acatctgatt tcaatctagc cattacaaat cttaaatcaa gtgttaaaca
     3901 tgt
//
LOCUS       DAWDRC010000378         3896 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9541_length_3896_cov_2.310596, whole genome shotgun sequence.
ACCESSION   DAWDRC010000378 DAWDRC010000000
VERSION     DAWDRC010000378.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9541_length_3896_cov_2.310596"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          944 a         1001 c         1046 g          905 t
ORIGIN      
        1 tttaaaaaac aacaatttct cttgaaatta ttaaagacat cataaaaatc ggccaaaaaa
       61 tatcttgtgc tattcgcaaa atctatggta actctttagg cattccttcg aacaagatgc
      121 aagaaaagac aaaatgacag cccttctacg agtgattagc ctggtcgtga ttagcgtggt
      181 ggtgattatt atcccaccgt gcggggctgc acttggacga ggaaaggctt agagatcaag
      241 ccttaacgaa ctaagacccc cgcaccgaaa ggtccggggg ttttttttga ccttaaaaac
      301 ataactgagg agcagacaat gaataacagc acaaaattct gtttctcaag attcaggacg
      361 gggaactaac tatgaatggc gcacagtggg tggtacatgc gttgcgggca cagggtgtga
      421 acaccgtttt cggttatccg ggtggcgcaa ttatgccggt ttacgatgca ttgtatgacg
      481 gtggcgtgga acacttgctg tgccgacacg agcaaggtgc agcaatggcg gctatcggtt
      541 atgcccgtgc taccggcaaa actggcgttt gcatcgccac gtctggtcca ggtgcaacca
      601 acctgataac cgggcttgcg gacgcgctgt tagattccat ccccgttgtt gctatcaccg
      661 gtcaggtatc cgcaccgttt atcggcactg acgcatttca ggaggtggat attctggggt
      721 tgtcgttagc ctgcaccaag cacagtttcc tggtgcaatc actggaagag ttaccgcgta
      781 tcatggccga agcgtttgac gtcgccagct ctggtcgtcc tggtccggtt ttggtcgata
      841 ttccaaaaga tatccagtta gccagcggcg atctggaacc gtggttcacc actgttgaaa
      901 acgaagtgac tttctcacat gccgaagtcg agcaagcgcg tcagatgctg gcaaaagcgc
      961 aaaaaccgat gctgtacgtt ggcggtggcg tgggcatggc gcaggcagtt tcggctttgc
     1021 gtgaatttct cgctgccaca aaaatgcctg ccacctgtac gttgaaaggg ctgggcgcag
     1081 tagaagcaga ttatccgtac tatctgggca tgctggggat gcacggcacc aaagcggcga
     1141 acttcgcagt gcaggagtgc gatctactga tcgccgtggg cgcacgtttt gatgaccggg
     1201 tgaccggcaa actgaacacc ttcgcaccac acgccagcgt tatccatatg gatatcgatc
     1261 cggcagaaat gaacaagctg cgtcaggcac atgtggcatt acagggtgat ttaaatgctc
     1321 tgttaccagc attacagcag ccgttaaata tcgatgactg gcagcaacac tgcgcgcaac
     1381 tgcgtgatga acatgcctgg cgttacgacc atcctggtga cgctatctac gcgccgttgt
     1441 tgttaaaaca actgtcggat cgtaaatctg cggattgcat cgtgaccaca gatgtggggc
     1501 agcaccagat gtgggctgcc cagcacatcg tccacactcg cccggaaaac ttcatcacct
     1561 ccagcggctt aggcaccatg ggtttcggtt taccggcggc ggttggcgca caagtcgcgc
     1621 gaccgaacga tactgtcgtc tgtatctccg gtgacggctc tttcatgatg aatgtgcaag
     1681 aactgggcac cgtaaaacgc aagcagttac cgttgaaaat cgtcttactc gataaccaac
     1741 ggttagggat ggttcgacaa tggcagcaac tgtttttcca ggaacgatac agcgaaacta
     1801 cccttaccga taaccccgat ttcctcatgt tagccagcgc cttcggcatc cctggccaac
     1861 acatcaccca taaagaccag gttgaagcgg cactcgacac catgctgaac agtgatgggc
     1921 catacctgct tcatgtctca atcgacgaac ttgagaacgt ctggccgctg gtgccgcctg
     1981 gtgccagtaa ttctgaaatg ttggagaaat tatcatgatg caacatcagg tcaatgtatc
     2041 ggctcgcttc aatccggaaa ccttagaacg tgttttacgc gtggtgcgtc atcgtggttt
     2101 ccacgtctgc tcaatgaata tggccgccgc cagcgatgca caaaatataa atatcgaatt
     2161 gaccgttgcc agcccacggt cggtcgactt actgtttagt cagttaaata aactggtgga
     2221 cgtcgcacac gttgctatct gccagagcac aaccacatca caacaaatcc gcgcctgagc
     2281 gcaaaaggaa tataaaaatg accacgaaga aagctgatta catttggttc aatggggaga
     2341 tggttcgctg ggaagacgcg aaggtgcatg tgatgtcgca cgcgctgcac tatggcacct
     2401 cggtttttga aggcatccgt tgctacgact cgcacaaagg accggttgta ttccgccatc
     2461 gtgagcatat gcagcgtctg catgactccg ccaaaatcta tcgtttcccg gtttcgcaga
     2521 gcattgatga gctgatggaa gcttgtcgcg acgtgatccg caaaaacaat ctcaccagcg
     2581 cctatatccg tccgctgatt ttcgtcggtg atgttggcat gggcgtaaac ccgccagcgg
     2641 gatactcaac cgatgtgatt atcgccgctt tcccgtgggg ggcgtatctg ggcgcagaag
     2701 cgctggagca ggggatcgat gcgatggttt cctcctggaa ccgtgctgca ccaaacacca
     2761 tcccaaccgc agcgaaagct ggaggtaact acctctcttc cctgttggtg ggtagcgaag
     2821 cgcgccgcca cggttatcag gaaggtatcg cattggatgt gaatggttat atctctgaag
     2881 gcgcaggaga aaacctgttt gaagtgaaag acggtgtgct gttcactcca ccgttcacct
     2941 cctccgcgct gccgggtatt acccgtgatg ccatcatcaa actggcgaaa gagctgggaa
     3001 ttgaagtgca tgaacaggtg ctgtcgcgcg aatccctgta cctggcggat gaagtcttta
     3061 tgtccggtac ggcggcagaa atcacgccag tgcgcagtgt agatggtatt caggttggtg
     3121 aaggccgttg cggcccggtt accaaacgca ttcagcaagc cttcttcggc ctcttcactg
     3181 gcgagaccga agataaatgg ggctggttag atcaagttaa tcaataaata caaaaaatgg
     3241 gacggcacgc accgtcccat ttacgagaca gacactggga gtaaataaag tatgcctaag
     3301 taccgttccg ccaccaccac tcatggtcgt aatatggcgg gtgctcgtgc gctgtggcgc
     3361 gccaccggaa tgaccgacgc cgatttcggt aagccgatta tcgcggttgt gaactcgttc
     3421 acccaatttg taccgggtca cgttcatctg cgcgatctcg gtaaactggt tgccgaacaa
     3481 attgaagcgg ctggcggcgt tgccaaagag ttcaacacca ttgcggtgga tgatgggatc
     3541 gctatgggcc acgggggcat gctttattca ctgccatctc gcgaactgat cgctgattcc
     3601 gttgaatata tggtcaacgc ccactgcgcc gacgccatgg tctgcatctc taactgcgac
     3661 aaaatcaccc cggggatgct gatggcttcc ctgcgcctga atattccggt gatctttgtt
     3721 tccggcggcc cgatggaggc cgggaaaacc aaactgtccg atcagatcat taagctcgat
     3781 ctggttgatg cgatgatcca gggcgcagac ccgaaagttt ctgactccca gagcgatcag
     3841 gttgaacgat ccgcctgccc aacctgcggt tcctgctccg ggatgtttac tgctaa
//
LOCUS       DAWDRC010000379         3877 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9590_length_3877_cov_2.721088, whole genome shotgun sequence.
ACCESSION   DAWDRC010000379 DAWDRC010000000
VERSION     DAWDRC010000379.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3877)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9590_length_3877_cov_2.721088"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2038..2047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1016 a          876 c         1044 g          931 t
ORIGIN      
        1 tggcgggtca attactgcat gcacttcatc gaactgatag tcgctgccct cttattatta
       61 ttgaaaatgc tcacctgaat caccgccgca tacttgatga tctccaacgc gcgatatctc
      121 ttattcctga cggtcaattt ttgctgatcg gcagacccga tcgaaaagtt gaacgtgatt
      181 ttaaaaaaca gggcattgaa cttgtctcga caggtcgtct gacggagcac gagcttaagg
      241 caagcatcct tgaaggtcag aatattgacc agcccgatct ccttttaacc gccagagttc
      301 tgaaacggat agctttatta tgtcggggag atcgcagaaa gctggcgctt gccggcgaaa
      361 caattagctt attacagcaa gccgagcaga ccagcgtttt cactgcaaaa caatggcgaa
      421 tgatttaccg cgtattaggt gataagcgcc cccggaaaat gcagcttgcc gtggtgatga
      481 gtggaaccat tctcgcactt acctgtggtt ggctactgct gtcctctttc acagctccgt
      541 tacccgttcc tgcgtggctg atacccgtaa cgccagtagt taaacaagat atgactaagg
      601 atattgctca tgtggtgatg cgcgatagtg aggcgctaag cgtgttatac ggcgtatggg
      661 ggtacgaggt tccagccgat tcggcctggt gcgatcaggc agtaagagcg gggctggtat
      721 gtaaaagtgg gaacgcttct ttacaaacat tggtagatca gaatttgccc tggatcgcat
      781 cgcttaaagt cggggacaaa aaattgcctg ttgtggtcgt tcgcgtcggt gatgctaccg
      841 ttgatgtact cgtagggcag caaacctgga cgctgacaca taaatggttt gagttggtat
      901 ggacgggtga ttatcttctg ttgtggaaaa tgtcgccgga aggggagagt accattacgc
      961 gtgatagcag cgaggaagag attctctggc tggaaacgat gttaaaccgc gcattgcata
     1021 tttcgacaga atcttcggca gagtggcgtc ccttgttggt agaaaaaata aagcagtctc
     1081 aagactgcca tcacttgaaa actgacggtg ttgttggctt ttcaacgtta gtacatctct
     1141 ggcaggtggc gggggagagt gcctacttat atcgggatga agcaaatatt tccccagaaa
     1201 caacagtgaa ggggaaatga catgtttgaa ttctatatcg ccgcgcgtga gcagaaagaa
     1261 acgggtcacc ctggtatctt ctcccgacaa aaacacagca ccataatata tgtcatttgt
     1321 ttattgctga tttgcctgtg gtttgcagga atggtgttgg ttggtgggta tgcccggcag
     1381 ctatgggcac tttggatagt aaaagcagaa gtcactgtag atgctgaaac gcccgctttt
     1441 aaacaatcaa cacaacacta ttttttcaaa aaacagccac tccccgtcgt cgaatccgtc
     1501 gaggaggaag atgaaccagg cgtcgcggtt gagaatgccc cctcatcttc tgaagacgaa
     1561 gaaaatactg tagaagaaag cgacgagaaa gccggtttaa gagagcgtgt caaaaatgcg
     1621 ctgaacgaac ttgagagata acccaaaggc tgtttacttt actagcaata cgcttgcgtt
     1681 cggtggttaa gtatgtataa tgcgcgggct tgtcgtagtt gacagcaggt tcaatctgaa
     1741 cctcagttag ccgaatttgg ctacctaaca atgctcccaa tcggggagct atgtaagaac
     1801 ggttacactc tcccatcaat cgtaatgggt ctgaggagta atcattttcg tttataaaat
     1861 aattggagct ctggtctcat gcagaaccaa agaatccgta tccgcctgaa agcgtttgat
     1921 catcgtctga tcgatcaagc aaccgcggaa atcgtcgaga ctgctaagcg cactggtgcg
     1981 caggtccgtg gtccgatccc gctgccgaca cgcaaagagc gcttcactgt tctgatcnnn
     2041 nnnnnnnacg tcaacaaaga cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg
     2101 gttgacatcg ttgagccaac cgagaaaacc gttgatgctc tgatgcgtct ggatctggct
     2161 gccggtgtag acgtgcagat cagcctgggt taatcaggtc attgagcgat tgagaggttg
     2221 aaacaatgat tggtttagtc ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg
     2281 gcgtttctat cccagtaacc gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag
     2341 acctggctaa cgatggctac cgtgctattc aggtgaccac cggtgctaaa aaagctaacc
     2401 gtgtgaccaa gcctgaagct ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc
     2461 tgtgggaatt ccgcctggct gaaggcgaag agttcactgt aggtcagagc attagcgttg
     2521 aactgtttgc tgacgttaaa aaagttgacg taactggcac ctctaaaggt aaaggtttcg
     2581 caggtaccgt taagcgctgg aacttccgta cccaggacgc tactcacggt aactccttgt
     2641 ctcaccgcgt tccgggttct atcggtcaga accagactcc gggcaaagtg ttcaaaggca
     2701 agaaaatggc aggtcagatg ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac
     2761 gcgttgacgc tgagcgcaac ctgctgctgg ttaaaggcgc tgtcccgggt gcaaccggta
     2821 gcgacctgat cgttaaacca gctgtgaagg cgtaaggaga tagcaatgga attagtattg
     2881 aaagacgcgc agagcgcgct gactgtttcc gaaactacct tcggtcgtga tttcaacgaa
     2941 gcgctggttc accaggttgt tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct
     3001 cagaagactc gtgctgaagt aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc
     3061 ggccgtgcgc gttctggttc tatcaagagc ccgatctggc gttctggtgg cgtgaccttt
     3121 gctgctcgtc cgcaggacca cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg
     3181 aaaagcatcc tgtccgaact ggtacgtcag gatcgtctga tcgttgtcga gaagttctct
     3241 gtagaagcgc cgaaaactaa gctgctggca cagaaactga aagacatggc tctggaagat
     3301 gtgctgatca tcaccggtga gctggatgag aacctgttcc tggctgcgcg caacctgcac
     3361 aaggttgacg tacgcgatgc aactggtatc gacccggtta gcctgatcgc cttcgacaaa
     3421 gtcgtaatga ctgctgatgc tgttaagcaa gttgaggaga tgctggcatg attcgtgaag
     3481 aacgtctgct gaaggtgctg cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg
     3541 aaaaatccaa caccatcgta ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag
     3601 ctgctgtgca gaaactgttt gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag
     3661 ggaaagttaa acgtcacgga cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg
     3721 tcaccctgaa agaaggccag aatctggact tcgttggcgg cgctgagtaa gtcggaggag
     3781 taatacaatg gcagttgtta aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa
     3841 agtggttaac cctgagctgc acaagggcaa acctttt
//
LOCUS       DAWDRC010000380         3875 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9595_length_3875_cov_2.323560, whole genome shotgun sequence.
ACCESSION   DAWDRC010000380 DAWDRC010000000
VERSION     DAWDRC010000380.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9595_length_3875_cov_2.323560"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          884 a         1029 c         1053 g          909 t
ORIGIN      
        1 attaagcgca tttcgtttat cccgttcgcc atttatcgct ttattgtggc ggctgcggtg
       61 tacgttgtgt tcttttgatg tgaagaacag tgtcggatat gatgctgacg cgtcttatca
      121 gattgcgtag gccggataag gcgttcacgc cgcatccggc aatcaacgcc tgatgcgacg
      181 ctcgcgcgtc ttatcaggcc tacaactgtc gttgacatgt agccggataa gacgcttacg
      241 tcgcatccgg caaaatatcc aacactcact caggttttgg gcaacgctgt tccttccagc
      301 cagccaccgc cgcaatccgc cgtcgggtca gctcctcgcg aatctccaca cctttaaatc
      361 ccgcttcaac gacggctttt gtcggcactg actgtgccac ttcccaggct tcgcgcaacc
      421 agcggccttg cgggtagtcg gctgattcaa aaccggttct gccgcgcaca tcagcctcgc
      481 tggtcagcgc cagttgctcg acacgctgcg gtttacgcca ggcgtcgatg gaatcaaata
      541 gtttgacgat ggtttttggg ttcagcattg ggaaggtgtg gatgagatcg tgaaactcag
      601 ccaccagtct ggctaaatcg cgaatttcat ttggcacgcg cagacgctgg catagttgtt
      661 ccactaactt cacacccgcc gggccatgac catgatgacg cggccaaagt tctggtggcg
      721 tcagtccttt accgagatcg tggcataacg ttgcgaaacg gacatcgacc tgcggactca
      781 gcatcgccgc cattgagagc gtcattaagg tatgaatacc ggtgtcgatt tccggatgcc
      841 acctggcagg ggccggaacg ccaaacaggg cgtcaatttc cgggaataaa acgcgcagtg
      901 cgccgcaatc gcgcagtacc tggaagaaca cctggggatt gcgggtggtg agggcgtttt
      961 ccgtctcttt ccatacccgt tcaggcgtca ggtgttccag ttcacccgca tgggtcatct
     1021 cgcgcatcag cgccagagtt tcatcggcaa tacggaaact gaggtgggca taacgcgcag
     1081 caaaacgcgc tacgcgcaac acgcgtaacg gatcttcgcc aaaagcgggg gaaacatggc
     1141 gcaacagacg attttgcaga tcgcccagac cgttgtacgg gtcgataatc tcgccgttat
     1201 cgtcctgggc cagcgcatta atggtcagat cgcgacgctt aagatcatct tccagcgtga
     1261 catccggtgc ggcatagcag gtaaaaccgg tgtaaccgga accggatttc cgttcggtac
     1321 gtgccagtgc atactcttca tgcgtttgcg gatgcagaaa cacaggaaaa tcgcggccta
     1381 cctgctggta gcccgcgtcg agcatctcct gtggcgtact gccgaccacc acccaatctc
     1441 tgtctttgac cggtagccct aacaatgcat cccgaacagc accaccgacc agataaatct
     1501 tcacgccatc atcccttcgt caaatacaca attcctaata ataagacagt gcaacgaaga
     1561 aggcgattta gttcatccag cgatctttgc gtttgcggct tgggatcagg tgcggcagta
     1621 ccagaccgag cagcaagcca agccccagca cgccaccgcc atacataaac cactgcatga
     1681 tgatggtgcg ctgtttgtca tccagctgta cgctggcggc atcgaccttt ttctgcgcga
     1741 caatcagctc gtttttcagt ttctgatttt cttcttttaa cccgttgatc acgctgtcgc
     1801 tctgcgccac tttttgctgc atttctgcgg tgcgctgatt ccaggtgtta tcgatattgg
     1861 tgagtttatc ggtcagggtt ttgacctgat tttccagatc tggcacacgg gagcgcaggc
     1921 ttggctcagt gctaagttgt ttcaacggga tccaggcggt acggccagag ctgtctttca
     1981 cctgggcata attggtgttg gcgtcagttt gtaataaggt cacttcctcg ccggcgttaa
     2041 ccgtgcccac gaggcgataa tgatctcccg gaccgctacg gacccaggta ttcagttcat
     2101 cagaaacata gcgcgtttct tcagcgtgtg agacggcagt ggcgctaagt gcgagtaaag
     2161 ttaatccgat caggcgtaat tttggcatca ggctgtcgtt attgtcatgg aaagtggaac
     2221 gatagtagtg gcatcagtgt cgctacgcaa agcattcgac atcaatcgga atcctctgtg
     2281 tcaggtcttg ccactgcttc aaacttttac gcccgtcaaa ttgcccgttg catggcaatt
     2341 tggcgcaaaa tactatctac tgacaaaaaa gatcgcatta agttcgccgt tcatcgtcac
     2401 ttatgtgaca taaccgataa gtaaaggcta tggctcagga aatcgaatta aagtttattg
     2461 ttaatcacag tgccgttgcg gcgctgcgtg accatctcaa tacgctgggc ggcgagcacc
     2521 atgaccccgt gcagttgctg aatatttact acgaaacgcc ggataactgg ctgcgtgggc
     2581 acgacatggg cttgcgtatt cgtggcgaaa acggtcgcta tgagatgacc atgaaagttg
     2641 caggaagagt gacaggcggc ttacaccagc gcccggaata taacgtggcg ttgagcgaac
     2701 cgacgctcga cctagcgcag ttaccgacgg aagtctggcc gaacggcgaa ttgcctaccg
     2761 atctcgcttc ccgcgtgcag ccgctgttta gcaccgattt ttatcgcgaa aagtggctgg
     2821 tagaggttga tggtagccaa attgaaatcg ccctcgacca gggggaagtg aaagcgggtg
     2881 aatttgctga acctatctgc gagctggaac tggaactgct tagcggcgac acgcgcgcgg
     2941 tgctgaaact ggcgaaccaa ctggtatcgc aaaccggatt acgccagggc agcctgagca
     3001 aagcagcgcg tggctatcat ctggcgcagg gcaatccggc gcgtgaaatc aaaccgacca
     3061 ccattttgca tgttgcggca aaagctgatg tggaacaggg gctggaagcg gcgttcgagc
     3121 tggcgttagc gcaatggcag tatcatgaag aactgtgggt acgcggcaac gatgcggcga
     3181 aagagcaggt gctggcagcc attggcctgg tccgtcatac actgatgctg ttcggcggta
     3241 tcgtgccgcg taaagcgagc actcacttac gtgatctgct gactcaatgc gaggcgacca
     3301 ttgcttctgc ggtttctgcc gtaacggcgg tttactctac cgaaacggca atggcgaagc
     3361 tggcgttgac cgaatggctg gtaagcaaag cctggcagcc gtttttagat gccaaagcgc
     3421 agagtaaaat gagcgattcc ttcaaatgct ttgccgatat ccatctttcc cgccatgctg
     3481 ctgaacttaa aagcgttttc tgccagccgt taggcgatcg ctaccgtgac caattgccac
     3541 gcctgacgcg tgatattgac tcaatactgt tgctggcggg ttactacgat cctgtcgtcg
     3601 cgcaagcctg gctggagaac tggcaggggc tgcgtcacgc tattgcgacc gggcaacgca
     3661 ttgaaattga acatttccgt aatgaggcaa acaatcagga accgttctgg ttgcacagcg
     3721 gaaaacgtta atcaggcaag gataaaatcg cttatgaagc cgctctcttc accgttacag
     3781 cagtactggc agaccgttgt tgagcggctg ccagagcctt tagccgagga atcacttagc
     3841 gcacaggcga agtcagtact tactttcagt gattt
//
LOCUS       DAWDRC010000381         3874 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9597_length_3874_cov_2.017020, whole genome shotgun sequence.
ACCESSION   DAWDRC010000381 DAWDRC010000000
VERSION     DAWDRC010000381.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9597_length_3874_cov_2.017020"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1509..1518
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          899 a          971 c          996 g          998 t
ORIGIN      
        1 ttgccgtgga tcagcaggtg cgccacttct tcaaattcac aatgttccgc cagatcaaga
       61 atatcgtagc cacggtaatg caggtcgttg ccgcttttac ccacggtgca gagtgccgta
      121 ttgcccgccg gaacgccgga aagtgccacc gattttttcg gtttaataac atgggtactg
      181 ttttgcagga tcgttgtgtc gctcatattg tcctcgtcat tgttatcatt tgtagggtcg
      241 agattgttgt cggatgcgac gctatgcgtc ttatccgacc tacagttcag ttcacctcgt
      301 aggcctgata agtcgcgtca gcggcgcatc aggcatcggt tgtcgaatac aaccaacggg
      361 cgtttttatt tcacctgacc acgggcaaac aggtcgtcga gcttctcttc gtactggtag
      421 tagttgatgc tttcgtacag ctcgttgcgg gtttgcatgg tgtcgatgac gcttttctgc
      481 gtgccttcct ggcgcaggac gttgtagaca tgttcagcgg cgcggttcat ggcgcggaaa
      541 gcagaaaggg gatacagcgc cattgcgaca tgggcgctgc gtaattcgtc ggtggtaaac
      601 agcggcgtgg caccaaattc agtaatgttg gcaaggatcg gcacctgcac cgcatcggca
      661 aactggcggt acatggcgag ttcggtaatt gcttccggga acagcatctc ggcacccgct
      721 tcaacatagg cctccgctcg ttcgattgcc gcatccagcc cctctaccgc cagcgcatcg
      781 gtgcgcgcca tgatcacaaa atcaggatcg gttttcgcat ccaccgccgc gcggatccga
      841 tccaccatct cttctttcga gacgatcgct ttattcggac gatgaccgca gcgtttcgca
      901 ccaacctgat cttcaatatg caatcctgcc gcaccggctt taatcattga tttcaccgta
      961 cgcgccacgt taaaggctga agaaccaaaa ccgatatccg catccaccag cagcggcagc
     1021 gaacaaacgt cggtgatacg gcgaatatct gtcagcacgt catcaagagt agaaataccg
     1081 agatcgggca gccccagcga ccctgccgcc acgccaccgc cggagagata aattgcctga
     1141 tatccggcac gctgcgccaa cagcgcatgg ttggcgttaa tggtgccaac aatctgcaat
     1201 ggattttctt tagtcagtgc agcgcgaaac gctttacctg gagagtgtag agacatagcc
     1261 catcctttgt tatcaacttg ttatttgcgt tgataaacac aaagcaaggg gtgtgccaca
     1321 attgactgaa taaaattaaa tgtttaattt caatttgtta taaataaagt catgaagtaa
     1381 tccgcaaata tgcgtttcag ttaacgtttc aggcaatgtt tcaccgcgtt tcattgcaac
     1441 aattatgaaa caagactaaa cccaatattc ggtttcttaa ctttgcggtg cgctatggca
     1501 catccaccnn nnnnnnnnat gacgaaaaac cggttatctg gacggtatct gtaacgcggc
     1561 tgttcgagct gtttcgcgat atcagcctcg agtttgatca tctggcgaac attaccccta
     1621 tccagcttgg ctttgaaaaa gcggtgacct acatccgcaa gaaactggca agcgaacgct
     1681 gcgacgccat catcgctgct ggctctaacg gcgcatatct taaaagccgc ctgtcagtgc
     1741 cagttatttt gattaaaccg agcggctacg atgtgttaca ggcactggca aaagccggaa
     1801 aactcacctc ttctatcggc gttgtcactt atcaggaaac tattccggcg ctggtggcgt
     1861 ttcaaaaaac ctttaatttg cgcctcgacc aacgtagcta cattaccgaa gaagacgcgc
     1921 gcgggcagat taacgagctg aaagctaacg gcaccgaagc ggtggtcggc gcggggctga
     1981 ttaccgatct ggcagaagaa gccggaatga ccggaatttt tatttattcc gccgccaccg
     2041 tacgccaggc gttcagcgat gcgctggata tgacgcgcat gtcgttacgc cataacactc
     2101 acgatgccac ccgcaacgcc ctgcgtactc gttacgtgct gggcgatatg ctcggtcaat
     2161 caccacagat ggaacaggtg cggcagacta ttttgctgta tgcccgctcc agtgcggcgg
     2221 tgttgattga aggggaaacg gggacgggca aagagctggc ggcccaggcg attcatcggg
     2281 aatattttgc ccgccacgat gcgcgacagg gcaaaaagtc acatccgttt gttgccgtca
     2341 actgcggggc gattgcagaa tcgctgctgg aagcagagct gtttggctat gaggaagggg
     2401 cgttcaccgg ctcgcgacgc ggaggtcgcg ccgggctgtt cgaaattgcc cacggcggta
     2461 cgctgtttct ggatgagatt ggcgaaatgc cgctaccttt gcagactcgc ctgttacggg
     2521 tgctggaaga aaaagaggtc acccgcgtcg gcgggcatca gcctgttccg gtagatgtgc
     2581 gggtcattag cgccactcac tgcaatctgg aagaagatat gcagcaaggg cagtttcgcc
     2641 gtgacctgtt ttatcggctg agtattttac gcctgcaact gccgccgcta cgcgagcggg
     2701 tggcggatat tctgccgctg gcggaaagct ttttgaaaat gtctctggcg gcactctctg
     2761 tccctttttc tgccgcatta cgtcagggat tagagacctg tcagattgta ttattgctct
     2821 acgactggcc gggcaatatt cgtgaactgc gcaatatgat ggaacgactg gcgctatttt
     2881 taagtgtgga atcgacgccg gatttaacgc cgcaattttt gcagctgcta ctgccggaac
     2941 tggcgcgcga gtcagcgaag actcccgttc caggcttgct gacagcacaa caggcactgg
     3001 agaaatttaa tggcgataag acagcagcgg cgaattattt aggtatcagc cggacgacgt
     3061 tctggcggcg gctgaaaagc tgaactgccg aatgcagcgc gaacgcttta tccggcataa
     3121 atttcaatag atgatgatca ccgataaact tacgcatcgg ggatgtttac ataggattca
     3181 gaattacttc ttcttataaa tatttgccgt gccgtggatc ttattgtcag tttgaccgga
     3241 ggtcagtacc aacacatcag cccctttttc atcggctttt ttgatcaaat cttctttcgc
     3301 atctgcggtc gacatttcat tactggttga aatatcacct attttttcat attgcgatgc
     3361 aaccttttca aattccgctt tggtcattaa ttctgcggca tagacattgg taacggcaaa
     3421 cgctaacgca ccgactaaca ttttagagat tattttcata ttacggctcc agagttgtta
     3481 tcacactatc acccacccac attattgggt tgattaataa ttagttcagt cagggcataa
     3541 acgccagtaa ccggaaataa gacactttgt gatctctctc gcacccgatt atttttggta
     3601 gaaatatgat gaagttactt cacttattta tggatgaaat cactatggat cctttgcacg
     3661 ccgtttacct gaccgtagga ttgttcgtga ttactttttt taatccggga gccaatctct
     3721 ttgtggtagt acaaaccagc ctggcttccg gtcgacgcgc gggggtgttg accgggctgg
     3781 gcgtggcgct gggcgatgca ttttattccg ggttgggttt gtttggtctt gcaacgctaa
     3841 ttacgcagtg tgaggagatt ttttcgctta tcag
//
LOCUS       DAWDRC010000382         3856 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9650_length_3856_cov_1.785057, whole genome shotgun sequence.
ACCESSION   DAWDRC010000382 DAWDRC010000000
VERSION     DAWDRC010000382.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9650_length_3856_cov_1.785057"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    789..798
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          955 a         1017 c         1080 g          794 t
ORIGIN      
        1 gcttgagcac atcctcaacg aagaacatat cgctcacgag ccgcgggcgc tgcaattgtt
       61 ggcacgcgcc gctgaaggca gcctgcgaga tgccttaagt ctgaccgacc aggcgattgc
      121 cagcggtgac ggccaggttt caacccaggc cgtcagtgcg atgctgggta cgcttgacga
      181 cgatcaggcg ctgtcgctgg ttgaagcgat ggtcgaggcc aacggcgagc gcgtaatggc
      241 gctaattaat gaagctgctg cccgtggtat cgagtgggaa gcgttgctgg tggaaatgct
      301 cggtctgttg catcgtattg cgatggtaca actttcgcct gctgcacttg gcaacgacat
      361 ggctgccatc gagctgcgga tgcgtgaact ggcgcgcacc ataccgccga cggatattca
      421 gctttactat cagacgctgt tgattggtcg caaagaatta ccgtatgcgc cagaccgccg
      481 tatgggcgtt gagatgacgc tgctgcgcgc gctggcattc catccgcgta tgcctctgcc
      541 tgagccagaa gtgccacgcc agtcctttgc gcccgttgcg ccaacggcag taatgacgcc
      601 aacacaggtg ccgccgcaac cgcaatcagc gccgcagcag gcaccgactg taccgctccc
      661 ggaaaccacc agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac
      721 caaagcaaaa aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc
      781 gctggaaann nnnnnnnnag cgcgacccgt gggcggctca ggtgagtcaa ctttcgctac
      841 caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac aacgcagtat
      901 gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggcgcacag caaaaactgg
      961 ctgaagcgtt gagcacgtta aaaggttcaa cggttgaact gactatcgtt gaagatgata
     1021 atcccgcggt gcgtacgccg ctggaatggc gtcaggcgat atacgaagaa aaacttgcgc
     1081 aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcga ttcttcgatg
     1141 cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt acgttcgtca
     1201 tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg taacctgatg
     1261 aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat cgcgcagctg
     1321 gaagtcaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg tgcacacaac
     1381 tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat gctggaagac
     1441 ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca gaaagaaaaa
     1501 atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc gttctgatgc
     1561 aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg ggcgttggcc
     1621 cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc ggcgggatgc
     1681 gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc gattgccgca
     1741 ccttcaccga acaggaagtc tgtaacatct gttcgaatcc acgtcgtcag gaaaacggtc
     1801 aaatctgcgt ggtggagagt ccggcggaca tttacgccat tgagcagacg gggcagtttt
     1861 caggtcgtta ttttgtgttg atgggacacc tgtcaccgct ggacggcatc ggtcctgatg
     1921 atatcgggct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact gaagtgatcc
     1981 tcgccaccaa ccccacggtt gaaggtgaag ctaccgctaa ctacattgcc gagctttgtg
     2041 cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggt ggcgaactgg
     2101 aaatggtcga tggcaccacg ctgtcacact cccttgccgg gcgtcataag attcgttttt
     2161 aagcaaacga gagcaggatc acctgctctc gcttgaaatt actctccctt gtccccatct
     2221 ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta catgaaagga
     2281 caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat gatccattct
     2341 ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc cgatgcggcg
     2401 gacaagctgc gtttccgcgc gctttctaac ccggacctgt acgaaggtga tggtgaacta
     2461 cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga taacggcgtg
     2521 gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc cggcaccaaa
     2581 tcattcctcg aatccctggg ttctgaccag gcgaaagaca gccagctgat cggtcagttt
     2641 ggtgttggtt tctactctgc gtttatcgtg gctgacaaag tgaccgtgcg tactcgcgcg
     2701 gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg tgaatacacc
     2761 gttgccgaca tcactaaaga agatcgtggt actgaaatca ccctgcatct gcgtgaaggc
     2821 gaagacgagt tcctcgatga ctggcgtgtg cgttccatca tcagcaaata ttccgaccat
     2881 atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag acggcgaaac cattatctcc
     2941 tgggagaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga aatcaccgat
     3001 gaagagtaca aagagttcta caaacacatc gcccacgact ttaacgatcc gctgacctgg
     3061 agccacaacc gcgttgaagg taagcaggag tacaccagcc tgctgtacat tccgtctcag
     3121 gcaccgtggg atatgtggaa ccgcgatcat aaacacggtc tgaaactgta tgttcagcgt
     3181 gtgttcatca tggacgacgc agaacagttc atgccgaact atctgcgctt cgtgcgtggt
     3241 ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca ggacagcacg
     3301 gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct ggaaaaactg
     3361 gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct ggtactgaaa
     3421 gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct gcgttttgct
     3481 tctacccata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt ttcccgcatg
     3541 aaagaagggc aggagaaaat ctactatatc accgcagaca gttacgcggc agcgaagagc
     3601 agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct ttccgaccgc
     3661 atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt ccagtctgtg
     3721 tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag cgcgaaagaa
     3781 gcggagaaag cactgactcc gttcatcgac cgtgtgaaag ccctgctcgg cgagcgcgtg
     3841 aaagatgtcc gtctga
//
LOCUS       DAWDRC010000383         3837 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9692_length_3837_cov_1.927287, whole genome shotgun sequence.
ACCESSION   DAWDRC010000383 DAWDRC010000000
VERSION     DAWDRC010000383.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9692_length_3837_cov_1.927287"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3150..3159
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          946 a          983 c         1025 g          873 t
ORIGIN      
        1 gtgtgattgc catccggcgc ggtgacgcag ctaacggcga tttcgctggc gttggcgtgc
       61 ttcatcgcat tcagcaccgc ttcgcgaata atttgcaaca aatgcacctg catttgcgca
      121 tccagcgcca gggttggcag acggcagtcg agggtcagtt tggcgctggt ttgattttgt
      181 aacgtatcca gcatttccct caacgcggag gggagatccg cctgctgcag cgtcaggcgg
      241 aaagtagtca acagctcgcg taactgccga taagcatcat tcaacgcctg ggaaaaatcg
      301 gccatgatac tttgtgcggt ggcgttatct tccggtatcg aacgcttcag taacgtcaac
      361 tggatacgta agtaagaaag tacctgagcc agcgagtcgt gcaattcgcg ggcgatggtc
      421 gcacgttctt ccatcaacaa taactgctga aaatgcttct gcgcctgatt aaagtacaaa
      481 ccgcgtccca gcatcgacga aacgctgtta agcagcggtt ctgatgatga aacgtgacta
      541 ttttgccagt gcagttcgcc gtaaaccgtc tcttgcattg tcaccggtaa aatctgcatc
      601 ggcaattccg ggtttggctg cccttcgcta atccgccagt tgtcaccgac atttaactcc
      661 agatattcag ccgcttcatt gtcgcgaaca atctgcaaaa tatggcggaa acaatgcaca
      721 tcaatctggc tggtgtttag cgcctgcgaa cactgataca acacctccag acgacgcttg
      781 gcctcgtgga gatcgcgggt cttttcttct actgacgcct ccagcgaacg gtacaattta
      841 tgcagttcgc tcgacatctg gttaaaggtt tttgcaagca ggccaagctc attcggcagg
      901 ctggtatcca gcggcggcga gtcgaactgc ccgtgttcaa tacgctgact ggcggtaacc
      961 agctgattca gcggggcaac cacctgatgg cgtatgcggc gcaaagtaaa aaagaccagc
     1021 gtgaaaatac cgatgccgcc agccagtgaa atcgccacca ccagcagcat tttacgttca
     1081 gcgtagtgct gtaaagccag cacgaacagg tctatctgat taacataatt attaatattg
     1141 gcctgatacc acggcaagtc gcccttgctg agccgatgat tcatctccag ccagttggca
     1201 ttcagatgcg catagcgagt ttttactgcc tctggcacgt accagacgtt gagattggtt
     1261 aataccggtg aatgcagtgc ctgctgaaat aactggcgat gtgcattgag ttgtggactg
     1321 ccactttgca agtcgtagcc caggcggtaa ctctgcatac gcagcgatcc ggcaatattg
     1381 atagcctcag cgtcgcgtaa actgctcgcc agcgttagca gagcgatacc cgtggaaaga
     1441 atcgacagca gcacaatgta aaaaaaggcc cgggccagac tggccgagac gggtcgttta
     1501 acaatcacac gcgtcttctc cacaaaaaat cattaatgtt ccagttcgct gaaacgaaat
     1561 ttagtcagtg atgggattat ttgatctgcc acagtttata gcgaaatagt tacttattct
     1621 gggcacatga acattgccga ttcccctgta agtcgggtaa taacaacact catataaaga
     1681 ataaggtttt tacaaccaaa aaagaaggtc gttatgaatc gttttattat ggccaacagt
     1741 cagcaatgtc tgggttgtca tgcttgtgaa atcgcctgtg tcatggctca caatgacgag
     1801 caacatgtcc tgagccaaca ccattttcat ccccgaatta cggttatcaa acatcagcag
     1861 caacgtagtg cagtgacctg tcaccattgt gaagatgcgc cctgcgcccg tagctgccct
     1921 aatggcgcaa tcagccacgt tgatgacagc attcaggtca atcagcaaaa gtgtattggc
     1981 tgtaaatcct gcgtggtggc ctgtccattt ggtacgatgc aaatcgttct gacacccgtt
     2041 gcggcaggaa aagtaaaagc cacggcgcat aaatgcgacc tttgtgcagg gcgcgaaaac
     2101 ggtcctgcct gtgtcgaaaa ttgcccggcg gacgcgctgc aactggtcac tgacgccgca
     2161 ctctccggca tggcgaaatc ccgccgcttg cgcaccgcac gtcaggaaca acaaccgtgg
     2221 catgccagta ccgcggcgca agaaatgccg gtaatgagta aagtcgagca aatgcaggca
     2281 acgcccgcgc gtggcgagcc agataaactg gcgattgaag cgcgcaaaac cggttttgat
     2341 gaaatttatc tgccattccg tgccgatcag gcacaacggg aagcctcgcg ctgccttaag
     2401 tgcggcgagc acagcgtttg tgaatggacc tgcccgctgc ataaccatat accgcagtgg
     2461 attgaactgg tgaaagccgg aaacatcgac gccgccgtcg agctttctca ccagaccaac
     2521 accctgccgg aaattaccgg acgcgtttgc ccgcaagacc gtttgtgtga aggtgcctgt
     2581 actattcgcg atgagcacgg cgcagtaact atcggtaaca ttgaacgcta catttcagat
     2641 caggcgttgg agaaaggttg gcgtcctgac ttaagccatg tcaccaaagt ggacaagcgg
     2701 gtggcgatta tcggtgcggg tccggcaggg ctggcctgtg cggatgtttt gacccgcaat
     2761 ggcgtagagg tgacggtgta cgatcgccat ccagaaatcg gtggcttgct cactttcggc
     2821 attccttctt tcaaactgga taaatccctg ctggcacgcc gtcgggaaat cttcagcgcg
     2881 atggggattc acttcgaact caattgtgaa gtgggtaaag atgtctcttt gaattcgctt
     2941 ttggaacaat acgacgcggt cttcgttggc gtaggcactt accgttccat gaaagcgggt
     3001 ttacccaatg aagatgcgcc gggcgtttat gacgcgctgc cgttcctcat cgccaatact
     3061 aaacaggtga tgggactcga agagctaccg gaagagccgt ttatcaatac tgccggactt
     3121 aacgtcgtgg tactgggcgg cggcgacacn nnnnnnnnna agaaagaggt gaagaacgcc
     3181 cgtgaagagg gggccaactt cgaatttaac gtccagccgg tggcgcttga actgaatgaa
     3241 caaggtcacg tctgcgggat tcgtttcctg cgcacgcgtc ttggcgagcc ggatgcccag
     3301 gggcgtcggc gtccagtgcc ggtggaaggc agtgaatttg tcatgccagc cgatgcggtg
     3361 attatggcgt ttggcttcaa tccgcacggg atgccgtggc tggagtcgca cggtgtaacg
     3421 gtagacaaat ggggccgcat catcgcggat gtggaaagcc agtaccgtta ccagaccacg
     3481 aatccgaaaa tcttcgccgg tggtgacgcc gtgcgtggtg cggatctggt ggttaccgca
     3541 atggcagaag gacgtcatgc ggcacagggg atcattgact ggctgggcgt aaaatcagtc
     3601 aaatctcact gatagcctgc gcagacaaac ccgacttcac agcgtaagat aattgttcat
     3661 ttcgcgctgt ggagtcggta tgacgcaaca aatcaccctc gttaaagaca aaattctctc
     3721 cgataactat ttcaccctgc acaacattac ttacgatctc acgcgcaaag atggcgaagt
     3781 tatccgccat aaacgtgaag tttacgatcg cggtaatggt gcgacgatcc tcctgta
//
LOCUS       DAWDRC010000384         3832 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9704_length_3832_cov_2.070426, whole genome shotgun sequence.
ACCESSION   DAWDRC010000384 DAWDRC010000000
VERSION     DAWDRC010000384.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9704_length_3832_cov_2.070426"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1087 a          952 c          909 g          884 t
ORIGIN      
        1 atgattacgc ttagcgaagc attgtgaagc agcaaaaata tcggttcatc aaagggagtc
       61 gtcatgcatt tacgtcatct gttttcatcg cgcctgcgtg gttcattact gttaggttca
      121 ttgcttgttg cttcatcatt cagtacgcag gccgcagaag aaatgctgcg taaagcggta
      181 ggtaaaggtg cctacgaaat ggcttatagc cagcaagaaa acgcgctgtg gctcgccact
      241 tcgcaaagcc gcaaactgga taaaggcggc gtggtttatc gtcttgatcc ggttactctg
      301 gaagtgacgc aggcgatcca taacgatctc aagccgtttg gtgccaccat caataacacg
      361 actcagacgt tgtggtttgg taacaccgta aatagtgcgg tcacggcgat agatgccaaa
      421 acgggcgaag tgaaaggtcg tctggtgctg gatgatcgta agcgcacgga agaggtacgc
      481 ccgttgcaac cacgtgagct ggtagctgac gataccacga acaccgttta catcagtggt
      541 attggtaaag agagcgtgat ttgggtcgtt gatggcgaga atatcaaact gaaaaccgcc
      601 atccagaaca ccggtaaaat gagtaccggt ctggcgctgg atagcaaagg caaacgtctt
      661 tacaccacta acgctgacgg cgaattgatt accatcgaca ccgccgacaa taaaatcctc
      721 agccgtaaaa agctgctgga tgacggcaaa gagcacttct ttatcaacat cagccttgat
      781 accaacaatc agcgtgcatt tatcaccgat tctaaagcgg cagaagtgtt agtggtcgat
      841 acccgtaatg gcaatattct ggcgaaggtt gcggcaccgg aatcactggc tgtgctgttt
      901 aacccagcgc gtaatgaagc ctacgtgacg catcgtcagg caggtaaagt cagtgtgatt
      961 gacgcgaaaa gctataaagt ggtgaaaacg ttcgatacgc cgactcatcc gaacagcctg
     1021 gcgctgtctg ccgatggcaa aacgctgtat gtcagtgtga aacaaaaatc cactaaacag
     1081 caggaagcta cccagccgga cgatgtgatt cgtattgcgc tgtaatagat atggcgaggg
     1141 tgaaaacgga aagaattgcc tgacttttat cacgccgcat ccggcacctt cgtcagtagt
     1201 ctgaatttgc ccggcacgta gccgggcaat tttgcaatta actcgacgtt tcaggcttaa
     1261 acacaatttc ctgtttttct tcatcttcct cgacgactgg cgcagtgctg tgaatttcag
     1321 caacgcgttt gcgcacacca aaccagccta taaccagcag aataccgata atcggcagtg
     1381 ccgcgatggt ataagtcccg ttcgggtaat cgaacgccat cagcaccagg acactcagta
     1441 aaaacagtaa tgtcagccag gaagtgaagg gcgcgccagg cagtttaaaa ctgacatccg
     1501 ctgctttgcc ttctttaatc gctttacgca ggcgcatctg gcaaacgatg ataaacgccc
     1561 atgaagcgat gattcccagc gacgcgaagt tcaacacaat ctcaaatacg cgtgacggca
     1621 ccagatagtt gaggaatacg ccgacgacat acacaaccag tgtcgccaga atcccggcat
     1681 acggcacatg ctgacgactc attttcgcca taaaactcgg tgcggaaccg cccatcgcca
     1741 tcgagcgcag aatacgcccg gtgcagtaca gacctgaatt caggctggag agggcagcgg
     1801 tcagcaccac aatgttcata atgctgccga tgtacggcac acccagttta gagaaaaacg
     1861 tcacaaatgg actttgcccc gcctgatacg cgctccacgg caataacata accagcaaca
     1921 ccacggagcc gacgtaaaac aggccaatac gccaaatcac actgttaatc gctttaggca
     1981 ccatggtctg cggatcttta cattctccgg ccgctgtacc caccatttca atggaggcaa
     2041 aagcaaacac tacgccctga atcaacacca gcgcaggcag caaaccgtgg gggaagaagc
     2101 cgccattatc ggtgattaaa tgaaagccag tggtgttgcc atccagcggc tgaccactac
     2161 cgaggaacac tgtacccacg accaaaaagg ttacaatggc gagcacttta ataagcgcaa
     2221 accagaactc catctccgca aaccatttca caccgatcat attcatggtg ccaacgatgg
     2281 tcagtgcagc gagcgcaaag acccactgcg gcacgccgcc aaacgcgccc cagtaatgca
     2341 tatacagagc gacggcggta atatcaacaa tccccgtcat cgcccagttg atgaagtaca
     2401 tccagccagc aacataagcg gccttctcac ccaaaaactc acgggcataa gagacaaaac
     2461 tgccactgga agggcggtgt aacaccagct cacccaatgc acgcagaata aaaaacgaaa
     2521 acaggccgca gatcaaatag accagtgcca gtgcgggccc cgccatttgc agtcgcgccc
     2581 ccgcgcctaa aaacaaaccc gtaccaatcg cgccgccaat ggcgatcatc tgtacctggc
     2641 gattgcccat cgctttgtga tacccctctt cgtgggcatt aagccagcgg cgtttcgcgg
     2701 cgtgttgatc tgaagtgtcg gtgttgtgtt tactcattgc tctccctgat tgctttaatg
     2761 aaaaagtcat ataagttgcc atgaacaatg tttattcatc ctggcgatga tagttatcac
     2821 cattattctt tatgggtata aaactatcgg cgcagcatcc tacccgttat cgataaacga
     2881 tgcaaaacat ccccttacaa tcctgaaggg gatttataca actgacgaaa aaatgataaa
     2941 tccttttgct ggataacctg tgtgctgtcc tacacttaat ctttaaaaga ttgtgagggg
     3001 catatgatta aggtgtatgg cgtacccggc tggggctcta caatcagtga gctgatgcta
     3061 acactggctg atattcctta tcagttcgtt gatgtcagtg gttttgacca cgagggaact
     3121 tcacgcgatt gattaaaaac cctgaatccg ttgtgtcagg ttccgacact ggcgctgtaa
     3181 aaataacgag attatgacgg agacagcggc gattgcattg atggttctcg atcgtcgccc
     3241 ggaccttgcg ccacctcttg ggcgcgccga acgtcaacag tttcaacggc tattggtctg
     3301 gctggttgct aatgtctatc caacgttcac tttcgccgat taccctaagc gttgggcatc
     3361 tgacgcacct gaacagttaa agaagaatgt aattgaatat cgcaaatcgc tttatatctg
     3421 gctgaattcg caattgactg ctgaaccgta tgtgtttgga gagcaactaa cgctggtgga
     3481 ctgttatctc tgcaccatgc gcacatgggg accgggacac gaatggtttc aggataatgc
     3541 cccgaacatt aatgcgattg ctgatgctgt atgtcagata ccgaaattac aagaagtact
     3601 aaaaaggaat gccattattt aacaataatg ccgaagatta atgttaataa atcagcggca
     3661 aagttaactg gacgtccatt tgtttgctat gaatagcaat taatgttggc acaactattc
     3721 atatggttga tgtgatttcg gtttttatat taaatatcaa tttaatataa tctgaaaata
     3781 aaacatcttt tcgcatgtag agaaacatgt cgatatgata gcgcagtgca ga
//
LOCUS       DAWDRC010000385         3825 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9717_length_3825_cov_2.081432, whole genome shotgun sequence.
ACCESSION   DAWDRC010000385 DAWDRC010000000
VERSION     DAWDRC010000385.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9717_length_3825_cov_2.081432"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3179..3188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          874 a         1049 c          976 g          916 t
ORIGIN      
        1 aacgttcaag gccttcagcg gcggtcagtt cgcttaagaa gcgttttttc tcttcgctat
       61 tgaaagtcgc gcgaccagac tcgatacgct gttggatcca gcgtttttct tcggtgctgg
      121 taatgtgcat atactcggca ccaatcgggc cacagtaggt ttgcttgagg gcttccagca
      181 gctcgccgag tttcatggtt tctttgccgc tggcaaatga accgacgttg aaggtctcct
      241 ggaagtctgc ttcggtcaga tcgtggaaag acggatccag atcggctact ttctcttgct
      301 gccacagtcc cagcggatcg agattcgcat gctggtgacc acggaagcgg tatgcgttaa
      361 tgagctgcag gactttaacc tgcttcacat tggtgtcagg gtcggagatc gttgaagagt
      421 aacgtgaagc gtctttcgcc aggcggcgga aatattcacg cgtttgagag tggaattgat
      481 ccggtttgac tcccgtacca ggtaactgct ggaacgtcga acgccagtta gcgtcaaccg
      541 agtcaggatc ggttaagaag tcttcataga gctgttctat ccagctctgg tttgcgccag
      601 agaggtaaga agagtccaac caggctttca aagcgctgtt ctgcatcgtg atcccttaag
      661 catctttttt atgcttactt cgccgtggat actaccacgc acatttcata tctgatgtgc
      721 gtgccggggt tcacttgttg cggacttgtc gtgttttctg cgtccgctaa ggaaccttta
      781 aaaactgtct tagtgtcagg ggcagttttt aaagatttcc tgcatttctt ttgtaggccg
      841 gataaggcat tcacgccgca tccggcgctg tttgcctgat gcgacgcttg tgcgtcttat
      901 caggcctacg gtttctgcat ttcttttgta ggccggataa ggtgttcacg ccgcatccgg
      961 cactggttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacggtttc tgcatttctt
     1021 ttgtaggccg gataaggcgt tcacgccgca tccggcactg gttgcctgat gcgacgcttg
     1081 tgcgtcttat caggcctggg tttacgcatt acgttgcaac aacatcgact tgatatggcc
     1141 gatggcgcgc gtcgggttca gccccttcgg acatacactg acgcagttca tgatgctgtg
     1201 acagcggaat acactgaatg catcgctcaa accgtcgagg cggctgtcag tctcggtatc
     1261 acggctatcg atcaggaaac gaaatgccgc taacaagcct gccggaacga taaacttatc
     1321 gggattccac cagaaagacg gacaagaggt tgaacaacat gcgcagagaa tacattcata
     1381 caacccgtcg agtttttcgc gctgctctgg catctgtaaa tgctcgcgag ctggcggatt
     1441 ttgtccatta ttcaacaggt aaggcttaat tttctcatat tgcgcataga attgtcccat
     1501 gtctaccacc aaatcgcgga tcaccggtaa acctggcagc gggcgaatca caatcttctt
     1561 gcccggctgg ttgagtgccg aaatcggggt aatacaggcc agaccattct taccgttcat
     1621 gttcagaccg tcggaaccgc acacaccttc acggcaggag cgacggaacg acaggctggg
     1681 atctttctct ttcagctgga taagcgcatc cagcagcatc atgtcacgac cttcttccgc
     1741 ttccagggtg taatcctgca tacgcggagc atcatcaaca tccgggttat agcgataaat
     1801 tgaaaactcg agtctcattt tcctgtctcc gcattagtaa gtacgaatct tcggcgggaa
     1861 tgccgggcgc agtttcggtt ccatgttgac gcttcggcgc gtcatggatt ccgactctgg
     1921 cagatacagg gagtgtcaca gccagttttc atgatcgcga tacgggaagt cgaagcggct
     1981 atgcgcgcca cggctttcgg tacggaagtt ggcagaaaca gccgttgcat acgccgtttc
     2041 catcaggtta tccagttcca ggcactcaac gcgctgggtg ttgaactcgc tggaagtgtc
     2101 atccagacgg gcatttttca gacgctcgcg gataactttc aactgctcaa gccctttcgc
     2161 catcgcatca ccttcacgga agaccgagaa gttatgctgc atacattctt gcagtgctct
     2221 acggatcgcc accggatctt caccgttacg gttattgttc cagcggttca ggcgatccag
     2281 agacgcttct acatcagact cgctggcatc gcgcagtgcg ccctgctcgg cgatagactc
     2341 ttgcagatgc agacctgccg cacgaccaaa tacgaccagg tccagcagcg agttgccgcc
     2401 cagacggtta gcaccatgta ccgatacaca agcgatttca ccaacggcaa acagccccgg
     2461 aacaaccaca tcttcgcctt tctcattcac agtcagcgcc tgaccggtca ctttggtcgg
     2521 aataccgccc atcatgtagt gacaggttgg gataaccgga atcggctctt tcaccggatc
     2581 aacgtgagcg aaggtgcggg agagttccag aatacccggc agacgggatt cgagaacttc
     2641 tttccccaga tggtcaagtt tcagttttgc gtgtggcccc cacggaccat cacagccgcg
     2701 gccttcacgg atttcgatca tgatggaacg cgccaccacg tcacggcccg ccaggtcttt
     2761 ggcgttcggc gcataacgct ccataaagcg ttcgccatgt ttgttcagca gataaccgcc
     2821 ttcaccacgg caaccttcgg tgaccagtac gcccgcaccg gcaataccgg tcgggtggaa
     2881 ctgccacatt tccatatcct gtaccggtac gcctgcacgg atagccatgc cgacaccgtc
     2941 gccagtgtta atgtgggcgt tagtggtgga ctgataaata cgccctgccc cgccagtcgc
     3001 cagcactgtc gcgcgagctt taaagtaaac cacttcacca gtttcgatgc acagtgcggt
     3061 acaaccgacc actgcgccat cctggttttt caccagatcc agcgcatacc actcggagaa
     3121 aatggtggtg tggtttttca ggttctgctg ataaagcgtg tgcaacagtg cgtgaccgnn
     3181 nnnnnnnnat atgttccagt tccagaatcg cttccggccc ggttttacac atatattcaa
     3241 tcgcgtcctg gtcaccgata tagtccgacc cttttacggt gtcgtacata tgccattccc
     3301 agttatcttc atgggtatta cccagcgcaa cggtaatacc accttgcgca gaaacggtat
     3361 gggaacgggt cgggaagact ttagagagca gcgcacaggt ctggccactc tgggaaattt
     3421 gcagcgccgc gcgcatacct gcgccgccag caccaatcac aactgcatca aattctctga
     3481 ctggcaattt catcacacac cccacaccac aacgaatcca taaatcacgt aaaccaccag
     3541 tgcaacgaca atcaccagtt gcagcatcag gcgcaaggcc agcggtttaa cgtagtcggt
     3601 caacacctgc cacatgccga tccaggcatg gatcaagata gaaaacagcg ccagcagggt
     3661 gaacactttg gtgaacgcag aggcgaagaa accaatccag acttcatatg tcagctcgcc
     3721 actggtagcg aaaaaaccga ccatataaat gatgtagagc gtcaggacga tagcggtagc
     3781 acgaacgagg atgaaatcat gtacgccatt gcgtcctaat gcgga
//
LOCUS       DAWDRC010000386         3816 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9735_length_3816_cov_2.093592, whole genome shotgun sequence.
ACCESSION   DAWDRC010000386 DAWDRC010000000
VERSION     DAWDRC010000386.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9735_length_3816_cov_2.093592"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1460..1469
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1990..1999
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          895 a         1057 c         1007 g          837 t
ORIGIN      
        1 accaccgcca tcaatgaata cacggtggat tgggtaataa agtgttccag caatgcttgc
       61 ataatgtgcc tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa
      121 gccgtcaatt atctgattcg ttaccaatta tgacaacttg gcggctacat cattcacttt
      181 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatactcg
      241 cgagaaatag agttgatcgt caaaaccgac attgcgaccg acggtggcga taggcatccg
      301 ggtggtgctc aaaagcagct tcgcctgact gatgcgctgg tcctcgcgcc agcttaatac
      361 gctaatccct aactgctggc ggaacaaatg cgacagacgc gacggcgaca ggcagacatg
      421 ctgtgcgacg ctggcgatat caaaattact gtctgccagg tgatcgctga tgtactgaca
      481 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg
      541 ccgcagtaac aattgctcaa gcagatttat cgccagcaat tccgaatagc gcccttcccc
      601 ttgtccggca ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc
      661 cgggcgaaag aaaccggtat tggcaaatat cgacggccag ttaagccatt catgccagta
      721 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg tgagcctccg gatgacgacc
      781 gtagtgatga atctctccag gcgggaacag caaaatatca cccggtcggc agacaaattc
      841 tcgtccctga tttttcaccc ccccctgacc gcgaatggtg agattgagaa tataaccttt
      901 cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa
      961 acccgccacc agatgggcgt taaacgagta tcccggcagc aggggatcat tttgcgcttc
     1021 agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag
     1081 acattgccgt cactgcgtct tttactggct cttctcgcta acccaaccgg taaccccgct
     1141 tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag
     1201 tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta
     1261 tgccatagca tttttatcca taagattagc ggatccagcc tgacgctttt tttcgcaact
     1321 ctctactgtt tctccatacc cgtttttttg gatggagtga aacgatggcg attgcaattg
     1381 gcctcgattt tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc accggtgaag
     1441 agatcgccac cagcgtagan nnnnnnnnna gcttagcgtc gaacagcgcg cagctgtggt
     1501 cgggattggc gttgacacaa ccggctcgac gcccgcaccg attgacgccg acggtaacgt
     1561 gctggcgctg cgcccggagt ttgccgaaaa cccgaacgcg atgttcgtat tgtggaaaga
     1621 ccacaccgcg gttgaagaag cggaagagat tacccgctta tgtcacacgc cgggcaacgt
     1681 tgactactcc cgctatattg gcggtattta ttccagcgaa tggttctggg caaaaatcct
     1741 gcatgtaact cgccaggaca acgccgtggc acaatctgcc gcatcgtgga ttgagctgtg
     1801 cgactgggtg ccagctctgc tttccggtac cacccgcccg caggatattc gtcgcggacg
     1861 ttgcagcgcc gggcataaat ctctatggca cgaaagctgg ggtggcctgc cgccagccag
     1921 tttctttgat gagctggacc cgatcctcaa ccgtcattta ccttccccgc tgttcactca
     1981 aacctggacn nnnnnnnnnc tggactgccg atattccggt gggcacctta tgcccggaat
     2041 gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc cggcggcgcg tttgactgcc
     2101 atatgggcgc agttggcgca ggcgcacagc ctaacgcact ggtaaaagtt atcggtactt
     2161 ccacctgcga cattctgatt gccgacaaac agagcgttgg cgagcgggca gtgaaaggta
     2221 tttgcggtca ggttgatggc agcgtggtgc ctggatttat cggtctggaa gcaggccaat
     2281 cggcatttgg ggatatctac gcctggtttg gacgcatact cagctggccg ctggaacagc
     2341 ttgccgccca gcatccggaa ctgaaagagc aaatcaacgc cagccagaaa caactgcttc
     2401 cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga tcacctgccg gtggtgctcg
     2461 actggtttaa cggtcgccgc acgccaaacg ctaaccaacg cctgaaagga gtgattaccg
     2521 atctgaacct cgccaccgac gctccgctgc tgttcggcgg tttaattgcc gccaccgcct
     2581 ttggcgcacg cgcaattatg gagtgcttca ccgatcaggg gatcgccgtc aataacgtga
     2641 tggcgctggg cggcatcgcg cggaaaaacc aggtcattat gcaggcctgc tgcgacgtgc
     2701 tgaatcgccc gctgcaaatt gttgcctctg accagtgctg tgcgctcggt gcggcgattt
     2761 ttgctgctgt cgccgcgaaa gtgcacgcag acatcccatc agcccagcaa aaaatggcca
     2821 gtgcggtaga gaaaaccctg caaccgcgca gcgaacaggc acaacgcttt gaacagcttt
     2881 atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca ctatcttcca acttccgccc
     2941 cggcacaggc tgcccaggcc gttccgactc tataaggaca cgacaatgac gatttttgat
     3001 aattatgaag tgtggtttgt aattggcagc cagcatcttt acggcccgga gactctgcgc
     3061 caggtgacgc aacatgcgga acacgttgtt aatgcactga atacagaagc gaagttgccc
     3121 tgcaaactgg tgctgaaacc gctgggcacc acgccggatg aaatcaccgc tatttgccgc
     3181 gacgcgaatt acgacgatcg ttgcgccggg ctgatagtgt ggctgcacac cttctccccg
     3241 gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac cgttgctgca attccacacc
     3301 cagttcaacg cggcgctgcc gtgggacagc atcgacatgg actttatgaa cctgaaccag
     3361 accgcgcatg gaggtcgcga gttcggcttc atcggcgcgc gtatgcgtca gcaacatgcc
     3421 gttgttaccg gtcactggca ggataaacaa gcacatgagc gtatcggctc ctggatgcgt
     3481 caggcggtct ctaaacagga tacccgtcat ctgaaagtct gccgttttgg cgataacatg
     3541 cgtgaagtag cggtcaccga tggcgataaa gttgccgcac agatcaagtt tggtttctcc
     3601 gtcaatacct gggcggttgg cgatctggtg caggtagtga actccatcag cgatggcgat
     3661 gttaacgcgc tggtcgatga gtacgaaagc tgctacacca tgacacctgc cacacaaatc
     3721 cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta ttgagctggg gatgaagcgt
     3781 ttcctggaac aaggtggctt ccacgctttc actact
//
LOCUS       DAWDRC010000387         3799 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9794_length_3799_cov_2.134615, whole genome shotgun sequence.
ACCESSION   DAWDRC010000387 DAWDRC010000000
VERSION     DAWDRC010000387.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9794_length_3799_cov_2.134615"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    893..902
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1940..1949
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3070..3079
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          980 a          954 c          949 g          886 t
ORIGIN      
        1 actacatcac cggctggttc agcagcccga ataaaaaaga acagccggaa gcctttgttg
       61 ccgaactctt tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact
      121 cttaccgcca ggcggtcaat gccttctata aaaccacctt caagacggcc aacaatcccg
      181 atgaagatat gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag
      241 agtcgttatt ttcacgcgtt gatgaaattc gagtcctgga gaaaaccacc gattccgccc
      301 gcattcatgt tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctgattttac
      361 agcggcgcga aggtaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac
      421 ttaagcagat tgaggcaaaa accgccgcca gattaaaaca atgagctgaa ttaaataaca
      481 attagccgga acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc
      541 tgttgttctg gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaaga tcaatcagct
      601 ggcgagcaat gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc
      661 attacgccca caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga
      721 tgcacaggac tatttcgact gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa
      781 tccccggcag cattctgtta ccggggatat tttttaacgt ccaaccgccg ctttagggcg
      841 tttcttcgca ccagcattct gcaggcgaga ttgcgtagac gacgcttttt ttnnnnnnnn
      901 nntgggtcgc catcggcgta tatttagtca acgccggacg ggtattgcgg ttctggcgac
      961 gagcttcacg catctcttca atggttggcg caggcactaa gcaatcgcga cggctgccga
     1021 tcagatgctt tttacccatc gcttccagcg cctgacgaat taacggccag tttgccggat
     1081 cgtggtaacg caacaacgct ttatgcaaac gacgctgctt gtcgcccttc ggtacgaaga
     1141 cgtcttcact cttataacca atcttcgcca gcgggttttt gccggtgtaa tacatggtgg
     1201 tcgagttagc cagcggcgac ggatagaagt tctgcacctg gtcgaggcgg aagcgatgct
     1261 ttttcagcca tagcgccaga ttcaccatat cttcatcacg cgtacccggg tgcgcggaga
     1321 tgaaatacgg gatcagatac tgttctttac ctgcctgttt cgagtaagta tcgaacagct
     1381 ctttaaagcg gtcatagctg cccatgcccg gcttcatcat cttcgataac ggcccttctt
     1441 cggtatgttc cggggcaatc ttcagataac cgccaacgtg atgggtcgcc agctctttga
     1501 tatagcgcgg atcttctacg gcgatgtcat aacgcacacc agaggcaatc aggatctttt
     1561 taatgccttt cagatcgcgc gcacggcgat agaggttaat cgtcggttcg tggttagtgt
     1621 ccatatgcgg acaaatatcc ggataaacgc acgacaaacg acggcaggtt tgctcagcgc
     1681 gtggcgattt gcaactcaac atatacatgt tggcagtagg cccaccgaga tcggaaatca
     1741 cgcccgtaaa acctggaaca gtgtcgcgga tcgcttcgat ctcattaata atcgaatctt
     1801 cggaacggct ctgaataatg cgcccttcgt gctcggtaat agaacagaaa gagcagccgc
     1861 caaagcagcc acgcataatg ttgaccgaga aacgaatcat ttcgtaagcc ggaattcggg
     1921 cattaccgta agccggatgn nnnnnnnnnt acggcagcgc aaagacgcta tccatctctt
     1981 cggtagaaag cggaatagca ggcgggttga tccatacata gcggtcgcca tgtttttgca
     2041 tcaatgcgcg ggcgcagcct gggttagttt cgtggtgcag aatgcgcgaa gcatgggcgt
     2101 acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag gttttttccc
     2161 acggtttcgg gcgtggtggc tgcacggtta cagctttggc ttcctgcttt ttcggagcta
     2221 ccggtttgtt atccgcgcac ggcaaatctt cgccatacgg atgcgggatt gggtcgattt
     2281 tccctggggt atcaagacgg gtggaatcca caccgctcca gcctggcaac gcctctttta
     2341 cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac
     2401 ggtgcgccac ttccaccagc ggacgctcac cgttaccaaa catcagcatg tcggctttcg
     2461 aatccaccaa cacggaacgg cgcacggtat cggaccagta atcataatgc gcggtacggc
     2521 gcaggctggc ctcaataccg ccgaggatca ccggcacatc tttccacgcc tctttacaac
     2581 gttgggtata aaccagtgtg gcgcgatccg gacgcttacc cgcgacatta tctggcgtgt
     2641 aggcatcgtc atgacgtaaa cggcgatcgg cggtatagcg gttgatcata gagtccatgt
     2701 tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag tcgtctttgc
     2761 tgctccagtc tggctgggcg atgatcccga cgcgaaagcc ctgcgcttcc agcatacgac
     2821 cacaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga
     2881 tgtcgcagct atcccagcca agttgatcca tctcttcacg tgacatcggc aaaaacggtg
     2941 ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga
     3001 tcagggagat agagctcatt ttgcttccag aaatggtaaa aaaataatca aaggccgggg
     3061 attatacgcn nnnnnnnnna tacgctgtat cacgcgcgga aatgaagtga tgttgatgaa
     3121 cggtggcgaa ataccctggt gaggtttttc actgcccgac ttgggcaagc acatttaagg
     3181 ctggcggtat cgtggcaact gccgctacta tttatgccga tcagcaacgt tcaaatcata
     3241 ttgcacgacg atcggctatc cgaggacacc cacagtaagg caaggtatgg aacaggttat
     3301 tacaaagcgc cgctattacg atattggtct acagattgaa gagctgctgt attcaggcgt
     3361 atttaaggcc ggagaacgct taccttcaga acgtgaactg agcgagcggt ttaataccag
     3421 ccgaactacc attcgtgaag ccatcattat gctggaactg aaaggcgtat tgaatgtgaa
     3481 gcaagggtca gggattttct ttgtcgactc caccgataag ctcaaccaga aatcattaat
     3541 gccctattca gaaattggcc cgtttgaact gttacaggca cgtcaggtaa ttgagagcaa
     3601 tattaccggt tttgctgcct gccagatcag ctttaacgag ttacaagagt tgaagaaaat
     3661 cattggatta caggaaaaag ccatcgctgc ggaaagtgat aaatttgaag acctggatca
     3721 ccgatttcat agcattattg ccgaagcgac gcaaaatcgg gtactcatca aacaagctgc
     3781 tgaattatgg cgggcagtt
//
LOCUS       DAWDRC010000388         3798 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9798_length_3798_cov_2.033930, whole genome shotgun sequence.
ACCESSION   DAWDRC010000388 DAWDRC010000000
VERSION     DAWDRC010000388.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9798_length_3798_cov_2.033930"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2319..2328
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3027..3036
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          775 a         1102 c          909 g          992 t
ORIGIN      
        1 acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc agacgaggat
       61 ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct
      121 gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc
      181 cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca
      241 gcgtcgcatc aggcaatcca tgcaggatgc gacataaacg ccttatcccg catggaaccc
      301 taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc gatcacatcg
      361 ccagtgcgga catcgttgta gttcttaacg ccgataccac attccatacc gttacggact
      421 tcgttaacgt catctttgaa gcgtcgcagg gactccagct cgccttcgta gataaccacg
      481 ttgtcgcgca gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacaa
      541 cctgcgatag caccaaattt cggtgatttg aacacgtcac gaacttccgc cagaccgata
      601 atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcgatc
      661 aggttataga tgacggagta gtaacgcaga tccaggcttt ccgcttcaat cactttacgt
      721 gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg
      781 gtagcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg
      841 tcagtagaca gtttcagcaa ggagtcggaa attgcttcca cagaaccctg tacgtctgcc
      901 ttcaggacga tattcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg
      961 agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg acgataaagt
     1021 gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt
     1081 acgccggaca ggccaaggat ttccaccgga atggacggac ccgcttccag cacttcctgc
     1141 cccagttcgt tacgcattgc acgaacacga ccgtattcga agccacacag aacgatatcg
     1201 cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg
     1261 aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccagc
     1321 acttccgcct gcagcaggat agcatccagc agttcatcaa taccggtacc cgcttttgca
     1381 gatacgtgta cgaactggct ttcgccgccc cactcttctg gcaggatgcc gtactgggag
     1441 agttcgttct taacgcgatc cggatcggct tctggtttat cgattttgtt cacagcaacc
     1501 accaccggaa cacccgccgc tttcgcgtgc tggatagctt cgatggtctg cggcattaca
     1561 ccgtcgtctg cagcaacaac caggactacg atgtccgttg cctgcgcacc acgtgcacgc
     1621 attgaagtaa acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt
     1681 tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga ggccactttc
     1741 gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc
     1801 acaaccggcg cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct
     1861 tccagctcgt tttcacgacg caggataact ttgtggccca tctcttcagc aaccagctgt
     1921 gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct
     1981 ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatggtt
     2041 tcgccgatca caacgtcacg gttaacggcc tgagctggct tctggaagcc ttgctgcagc
     2101 gaagaacctt tacgttttcc gcccttaccg ccacgtactg ctgcgcgagc ttcttcacga
     2161 tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga
     2221 ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt
     2281 tgagaagtag tgacgtgata atcgctggaa tcttcagtnn nnnnnnnngc tttttcagcc
     2341 tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc cgcagcttca
     2401 cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg ttcagctttt
     2461 tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc ttccgcttca
     2521 cgctgcgctt gctcttccgc tgcaaggcgc tcagcctctt gcggatcgcg tttcacaaag
     2581 gtgcgtttct tgcggacttc gatttgtacc gatttgcttt ttccaccggt accaggaatg
     2641 ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt ccgggcctga atttttctga
     2701 ttcaagtggt caatcaaagt ctgtttctct tgtgcagaca cagagtcgtc agcagacttc
     2761 cggatacctg catcagcaaa ttgctgtacc aggcgttcca cggaggtctg tcgctctgcg
     2821 gccagcgttt taatcgttac atctgtcatg ctgttccttc ctgctacagt ttattacgct
     2881 tcgtcaccga accagcaaat attacgggca gccataatca gtgctccggc tttttcgtcg
     2941 gtcaaccctt cgatatcagc cagatcatca atgccctgtt cggcgagatc ttccagcgta
     3001 caaacgccac gggcggccag tttgaannnn nnnnnncttc aaggttcagc agatcgtcag
     3061 ccggtttgtt atcaccgagg ctttcttcct gggcctgtgc aatggtggcc agtgcatttt
     3121 tagcacgctc gcgcagtgct tcaacggtcg gctcatcaag gccttcgatt tccaacagct
     3181 ctttcatcgg cacataggcc agttcttcca gcgtcgagaa gccttcttct accagaacag
     3241 tcgcgaagtc ttcatcgata tcgagatatt tggtgaaggt gtcgatcgct gcgtgcgctt
     3301 ccgcctgatg cttagcctgc aggtcgtcaa cggtcatcac gttgagttcc caaccgctca
     3361 gttgcgaagc cagacgcacg ttctgaccgt tacggccaat cgcctgcgcc aggttaccgg
     3421 cttcaacggc gatatccatg gtgtgtttat cttcatccac cacgatagaa gcaacgtctg
     3481 ccggtgccat tgcgttaatc acaaactgcg ccgggttatc atcccacagg acaatatcga
     3541 tacgctcgcc gcccagttca gtagaaaccg cctgaacacg cgcgccacgc atacctacgc
     3601 aagcacctac cggatcgata cgtttatcgt tggttttcac cgcaattttc gcacgagatc
     3661 ccgggtcgcg agccgctgct ttaatttcaa tcacttcttc gccgatttcc ggcacttcaa
     3721 tacggaacag ttcgatcagc atttccggct tggagcgggt gacgaacagc tgcgcgccac
     3781 gcgcttccgg gcgaacgg
//
LOCUS       DAWDRC010000389         3794 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9809_length_3794_cov_2.055362, whole genome shotgun sequence.
ACCESSION   DAWDRC010000389 DAWDRC010000000
VERSION     DAWDRC010000389.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9809_length_3794_cov_2.055362"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    984..993
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2650..2659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3579..3588
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          919 a          979 c          892 g          974 t
ORIGIN      
        1 tcatcatttt gcagtatcct taagatattc cttatatctt caggagaaag tcatggacag
       61 attcccacgt tctgattcaa tcgtacaacc ccgggctggc ttgcaaactt atatggcgca
      121 agtctatggc tggatgaccg ttggcttgtt gctgaccgca tttgttgcct ggtatgcggc
      181 taactccgcg gccgtgatgg agctgttgtt cactaaccgt gtctttttaa tcggtctgat
      241 catcgcgcaa ttagcattgg ttattgtgtt atcagcgatg attcaaaagc tgagcgcagg
      301 tgtaacgacg atgctcttta tgctttattc ggcgctgacg ggtcttacgc tttccagtat
      361 tttcattgtc tatacggctg cttctatcgc cagtactttc gtcgttactg ccgggatgtt
      421 cggcgcaatg agcctgtacg gttacaccac gaagcgcgat ctaagcggct ttggcaatat
      481 gctgtttatg gcgttaatcg gcattgtgct ggcatcgctg gttaacttct ggctgaaaag
      541 cgaagcattg atgtgggcag tcacctacat cggcgtgatt gtctttgtcg gattgacggc
      601 gtatgacacc cagaaactga aaaatatggg tgagcagatt gatactcgcg acacgtcgaa
      661 cctgcgcaaa tattccattc ttggcgcgtt aaccttgtat ctggacttca tcaacctgtt
      721 cctgatgttg ctgcggatct tcggcaaccg ccgttaattc tttttcatca tcaatatccc
      781 ggccatgtgt cgggattttt ttcttattaa aaacgacact taacaagtac caggtataaa
      841 atatgcctta tggacgagtg gaagcgtttt ctgtatccat aaatgcaaaa gtattttgta
      901 tgcgtagttt taaatttatt gatggtgaat attaatgttt tttctaagga caactaaaag
      961 aatggaatca tactcgcaaa atannnnnnn nnngctatag gattattatt actttctatg
     1021 tatggcttaa gtaaagatat caacaagata aataccgcca tcgcgggcgt aatatatttg
     1081 tttttaatct ctttggtggc gattgtcgtt gcgagtttag cgcctgtatc tgccattatc
     1141 gtcgtgttca gcactgcggg atcgatgttt ttaatcagta tgctggcagg tctattgttt
     1201 aaatttgatc ctggttctca tcgttttatc attatgatga cgttgacagg gttggccctg
     1261 gtaatcatcg tgaatgccac attaatgagt gaacgaccca tttgggtaat aagttgctta
     1321 atgattgtct tatggtcagg cattatctcg catggacgaa ataagctcct tgaactgaca
     1381 ggaaaaagcc atagtgaaga attatggagt tcggttcgtt gtgcttttat aggtgcatta
     1441 acactctatt actattttat cggcttcttt gggattcttg ccactatagc tataacgctt
     1501 gtctggcaaa ggcatacgcg gttttttcat tagccgtaaa tgcacatact aaagaactta
     1561 actatacttc acatcgccgc tttatttttc gcccgtagct tcttcgcctg gctttccagc
     1621 aacaggtaac agatcagcgc cagcagcagt gggataaagt aatacagcac acggtaagcg
     1681 agcagggcgg cgataattgt gcctttggag gtatgctccc cagccagtag cgcgataaac
     1741 accgcttcca gcacaccaat tcccgccgga atatgcacga tgacgccagc aatactacta
     1801 accagcaaca cgcccagtac aaagaaatag ttcacgcttt gaccaagtaa cagccagata
     1861 atcgctccca ttaccatcca gttaacgctg gaaatcagca tctgggcgag ggcgaatttc
     1921 cacgaaggca gcaccagctt ttgccctttg atggtcatat ggcggtgctt cgcgaaagcg
     1981 caaaaccaca aataaaccgc gataatcatc agtaagccaa taccgagaat acgcagcgta
     2041 gtttgatcga cataccagtg atccggcaac tccaccacgc ctgcggtaaa gataaccccc
     2101 gctagcaaaa tatagcctag ccagttggtg gtaatactga gcgaaaaaat ccgcgtaata
     2161 gtgctgcccg gtaaccccag tcgggagtac aaacgatagc gcataccaat gccgccgacc
     2221 caggtactga gtgtcaggtt gaaggcgtag cagataaacg acaccagcat cacctggcgc
     2281 ttcgccagtt tgtgaccgca gtagaaacgg gcgagcaggt cgtagcaacc gtaaatcaga
     2341 taactgacga ccaccagccc gaccgcacta agcagcgcaa cgcgattgta gtcgcggatg
     2401 accttccaga cctcttccca gtccactttt ttggcgtaga ccaccagcaa cacaatcacc
     2461 gcgataaaaa acagccaggt gaggagtttc tttgctaagc gccagcgcgg gtgtgattta
     2521 ctcatcaggg ttttaccccc gtgttttcag tttctacccg atcctgcgtt tccattgtcg
     2581 gttgtgcggg gggaccaacc tgcgccagac gtggcgtgtg cgccggaagc cagcccacca
     2641 gcgccgggan nnnnnnnnng cccagtgatc gtccatcaat gccactttgc cgtggagcgg
     2701 gcggcggcgg tactcaaaca cctgaacgcc gcctttaacc agatagttat acagcaaacg
     2761 tgcgccgact ctgacaatcg gcatatccgg ttcgccctga atgatcagtt tgatccgcac
     2821 cccgcgccgt gccgctttac gcaaggcgtg taaaaaacga tagccgggga agaagtaggc
     2881 gttggcgata atcacttccc gccgcgcttg agtgagcatt ttcagatagt gacgttcgat
     2941 atcatcgcga tgttcttcgt tatcgcgcca gaccagcaat acctgcgctt ctcccggctg
     3001 gcggttctct tccgctttat gatgacgtcg ccaccagcgt cgcgccgcgc tctgtcctgg
     3061 caggttttcc agctcaaact ggagaatatc ttcgacaatc ggcccttcga ggcgtaccgc
     3121 gtaatcctgt ttggcctccg ggccgtagct ggacatatgc tcggcggagt aattcagccc
     3181 gccaataaag gcgatacgcg cgtcgatcac cacaattttg cgatgcatcc ggcgaaacac
     3241 attggtgcgc ataccaaaaa ggcgagggcg gggatcgtag tagcggaaca ccacgccagc
     3301 tgccgtcagt tcattgacaa actcatcgct gagatccggc gaaccgtagc catccagcaa
     3361 gacttccgct tttaccccgc gttgcgctgc tgccaatagc gccgcatgca gctgtttgcc
     3421 gacgtcatcc tcaaaccaga taaacgtttc aagaatgatg cgttcttgcg cctcgccaat
     3481 cgccttaaac accgcgggat aatattgctc gccgttttcc agcaactgga tcttattgcc
     3541 ttcgcgccag ctacatttca taaatgaatc tccgcactnn nnnnnnnnca cccgctacca
     3601 ataccggttc gccgtccggt agctcattca cccattcggc gagcatcgca agctgcgcct
     3661 gacggtgcgc ctcacacagg cccagatgta cgcacatcac atgaatcgct ttcccggtca
     3721 tcggcggcac aatgcggcag tagagcacgc cgcgcttttc cgcaccatcg accgaaacat
     3781 cacgattctc ataa
//
LOCUS       DAWDRC010000390         3794 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9807_length_3794_cov_2.624766, whole genome shotgun sequence.
ACCESSION   DAWDRC010000390 DAWDRC010000000
VERSION     DAWDRC010000390.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9807_length_3794_cov_2.624766"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          983 a          905 c         1003 g          903 t
ORIGIN      
        1 tatattttat gtgggtaatt atttaaataa gagaaacgtt tcgctggtaa tcaaacaaaa
       61 aatatttgcg caaagtattt cctttgtcat aaaaataata cttccagaca ctatgaagtt
      121 gtgaaacata atgttaactt ctccatactt tggataagga aatacagaca tgaaaaatct
      181 cattgctgag ttgttattta agcttgccca aaaagaagaa gagtcgaaag aactgtgcgc
      241 gcaggtagaa gctttggaga ttatcgtcac ggcaatgctt cgcaatatgg cgcaaaatga
      301 tcaacagcgg ttgattgatc aggtagaggg ggcgctgtac gaggtaaagc ccgatgccag
      361 cattcctgac gacgatacgg agctgctgcg cgattacgta aagaagttat tgaggcatcc
      421 tcgtcagtaa aaagttaatc ttttcaacag ctgtcataaa gttgtcacgg ccgagactta
      481 tagtcgcttt gtttttattt tttaatgtat ttgtacatgg agaaaataaa gtgaaacaaa
      541 gcactattgc actggcactc ttaccgttac tgtttacccc tgtgacaaaa gcccggacac
      601 cagaaatgcc tgttctggaa aaccgggcag ctcagggcga tattactgca cccggcggtg
      661 ctcgccgctt aacgggtgat cagaccgccg ctctgcgtga ttctcttagc gataaaccgg
      721 caaaaaatat tattttgctg attggcgatg ggatggggga ctcggaaatt actgccgcac
      781 gcaattatgc cgaaggtgcg ggcggctttt ttaaaggtat cgatgcctta ccgcttaccg
      841 ggcaatacac tcactatgcg ctgaataaaa aaaccggcaa accggactac gtcaccgact
      901 cggctgcatc agcaaccgcc tggtcaacag gtgtcaaaac ctataacggc gcgctgggcg
      961 tcgatattca cgaaaaagat cacccaacga ttctggaaat ggcaaaagcc gcaggtctgg
     1021 cgaccggtaa cgtttctacc gcagagttgc aggatgccac gcccgctgcg ctggtggcgc
     1081 atgtgacctc gcgcaaatgc tacggtccga gtgcgaccag tgaaaaatgt ccgggtaacg
     1141 ctctggaaaa aggcggaaaa ggatcgatta ccgaacagct gcttaacgcc cgtgccgatg
     1201 ttacgcttgg cggcggtgca aaaacctttg ctgaaacggc aaccgccggt gaatggcagg
     1261 gaaaaacgct gcgtgaacag gcacaggcgc gtggttatca gttggtgagc gatactgcct
     1321 cactgaattc ggtgacggaa gcgaatcagc aaaaacccct attaggactg tttgctgacg
     1381 gcaatatgcc agtgcgctgg caaggaccga aagcaacgta ccacggtaat atagataagc
     1441 ccgcagtcac ctgtacgcct aatccgcaac gtaatgacag tgtaccgacc ctggcgcaga
     1501 tgaccgacaa agccattgaa ttgttgagta aaaatgagaa aggctttttc ctgcaagttg
     1561 aaggtgcgtc aatcgataaa caggatcacg ctgcgaatcc ttgtgggcaa attggcgaga
     1621 cggtcgatct cgatgaagcc gtacaacggg cgctggaatt cgctaaaaag gatggtaaca
     1681 cgttggtcat agtcaccgct gatcacgccc atgccagcca gatagttgcg ccggatacca
     1741 aagctccggg cctcacccag gcgctaaata ccaaagatgg cgcagtgatg gtgatgagtt
     1801 acgggaactc cgaagaggat tcacaagaac ataccggcag tcagttgcgt attgcggcgt
     1861 atggcccgca tgccgccaat gttgttggac tgaccgacca gaccgatctc ttctacacca
     1921 tgaaagccgc cctggggctg aaataaaacc gcgcccggca gtgaattttc gctgccgggt
     1981 ggtttttttg ctgttagcaa ccagacttaa tgacagatca cgggcgcata cgctcatggt
     2041 taaaacatga agagggatgg tgcgatgaaa attacattac tggttacctt gcttttcggt
     2101 ctggtttttt taaccaccgt cggcgctgcc gagagaacct taaccccaca acaacagcgt
     2161 atgacctctt gtaatcagca ggcgacggcg caggcgttga aaggggatgc tcgtaagacc
     2221 tacatgagtg attgcctgaa gaacagcaaa tctgcgcctg gtgaaaaaag tttgacgcca
     2281 cagcagcaaa agatgcgcga atgtaataat caagcaacac aacaatctct gaaaggggat
     2341 gatcgtaata agtttatgag tgcctgcctc aagaaagccg cctgatacct gatagtgcta
     2401 acgggtgagc tacgaaaatg gctcacccga aatttcatac ttctgccttt agtcccgtct
     2461 ctataatttg ggaaaattgt ttctgaatgt tcccaaaaat aatgaatgat gaaaactttt
     2521 tcaaaaaagc ggcggcgcat ggggaggaac ctcctttaac tcctcaaaac gaacatcagc
     2581 ggtccgggct gcgcttcgcc cgtcgcgtca gactaccccg cgcggttggc ctggctggca
     2641 tgttcttacc gattgcttca acgctggttt cgcatccgtc gccgggctgg tggtggctgt
     2701 tgttggtcgg ctgggcgttc gtctggccgc atttagcctg gcaaatagcg agcagggccg
     2761 tcgatccgct tagccgggaa atttacaact taaaaaccga tgcagtatta gcgggaatgt
     2821 gggtaggcgt aatgggcgta aacgcactac cttccaccgc gatgttgatg attatgtgtc
     2881 tgaatttgat gggggcaggc ggcccccgtc tgtttgtcgc gggtctggtg ttgatggtgg
     2941 tttcctgcct tgtcaccctc gagttgacgg gcatcaccgt gtcgttcaat agcgcaccgc
     3001 tggaatggtg gctctccctt cccattattg tcatttatcc tctgctgttt ggctgggtca
     3061 gctaccagac ggcgactaaa ctggcggaac ataaacgcag gttgcaggtc atgagtaccc
     3121 gcgacggtat gacgggcgtg tataaccgac gtcattggga aactatgtta cgcaatgaat
     3181 ttgataactg tcggcggcat aatcgcgatg caacgttact gattatcgat atcgaccatt
     3241 tcaagagcat caacgatacc tggggccatg atgtgggtga tgaagcgatt gtggcgctta
     3301 cccgacagtt acaaattacc ctgcgcggta gcgatgtgat tggtcggttt ggcggcgatg
     3361 agtttgcagt aatcatgtcc ggtacgccag ctgagagcgc catcaccgcc atgttacggg
     3421 tgcatgaagg gctaaacacg ttacgcctgc cgaatacgcc gcaggtgatg ttacggatta
     3481 gtgtgggggt tgcgccgctg aacccacaaa tgagtcacta tcgtgagtgg ttgaaatcgg
     3541 cagatttggc gctttacaaa gcaaagaaag ccggacgtaa ccgcaccgaa gtggcggcct
     3601 gacgtccggc aaaagtcatc aggatttact gagtttttct gatttttcca tacacttcgt
     3661 catcgcttcg atcactgcgg cacggaagcc tttctcttcc agtacgcgta ccgcctcaat
     3721 ggtggtgcct cccggtgagc agaccatatc tttcagtgcc cctggatgtt ctcccgtttc
     3781 cagcaccatt tttg
//
LOCUS       DAWDRC010000391         3779 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9846_length_3779_cov_1.556391, whole genome shotgun sequence.
ACCESSION   DAWDRC010000391 DAWDRC010000000
VERSION     DAWDRC010000391.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9846_length_3779_cov_1.556391"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    816..825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1901..1910
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3044..3053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          903 a         1047 c          889 g          910 t
ORIGIN      
        1 atattgggcc gttggtggag atataagtgg atcacttttc atccgtcgtt gacactaata
       61 tctgcacatc aatgaaaata caaacaactt ccggcaaaaa agtgatacaa gatcgatgac
      121 atagcgttga agaaaaggat ttgataatcc gcaaaatttg cacaacactg cctgtaatac
      181 ttcctgaacc agagatgatc atgaccgtaa ctcgcccacg cgccgaacgc ggcgcatttc
      241 cgcccggaac agaacattac ggacgttcat tattgggcgc gccgttaatc tggtttccgg
      301 cccctacagc cagtcacgaa agtggtttga ttctggctgg cactcacggt gatgaaaact
      361 cttcgatcgt cacgctctcc tgtgctctgc ggactttgac accttctttg cgccgccatc
      421 atgtggtgct gtgtgtgaat cctgacggct gccagttagg gttacgggcc aatgctaatg
      481 gtgtggattt aaaccgaaac tttccggcag caaactggaa ggaaggtgaa acagtttatc
      541 gctggaacag cgccgctgaa gaacgtgatg ttgtattact gacaggtgac aaacctggct
      601 ccgaacctga aacccaggcg ctgtgccagc ttatacaccg cattcaacct gcctgggtgg
      661 tctcattcca cgatccgctg gcctgtattg aagatcccag acacagcgaa ttaggtgaat
      721 ggctggccca ggcgtttgaa ttgcctctgg taaccagcgt cggttatgaa acgcctggct
      781 ctttcggcag ttggtgtgca gatctcaact tgcatnnnnn nnnnntcaag atcggcgaaa
      841 ctgacctgcg gcaccagcgg taaagcggcg ctaatggcac gagaggtaca caacatgcag
      901 cccagcatca gacgaaaacc ttgtgcacgc gcttcagtcg ccagcgccag cgcttccgtc
      961 agaccaccgg ttttatcaag cttaatgtta accatctcat agcgcccttt cagcgccttc
     1021 agattgctac gagtatgaca actttcatca gcacaaatcg gtaacggatg aataaaattc
     1081 tccagcgccg catcgtcctg cgcaggaagc ggttgttcaa gcatcgcaac gcctaaatcc
     1141 gccaatagct ggcaacgcgc cgccaaccct tctgcacgcc aggattcatt tgcatcaacg
     1201 atcagcgtcg catcgggtac agctgtgcga attgccacca tccgctcact gataagatgg
     1261 ttatccagct tcactttcag taatttcgcg cctgcctgcc agagtgttga tgcactattg
     1321 gccatctgat caggcgtacc gatgactatc gtctgtgccg tggtgactgt ctcgggaagc
     1381 gttatgccga tcaaatcagc cagcgattgc tgctgttttc gcgccgccag atcccacaat
     1441 gcacaatcca gcgcattacg tgctgcgccg gcagggagga ttttttgcaa ctcctcccgt
     1501 gtcagccctt tctctaattg cggcacgacg ctcataattt gcgccattac cgaggcatca
     1561 ctttccccat aacgcggata cggcgtgcat tcgccggtgc ctttaatacc ttcttcttcc
     1621 agttcaacca ccaccacgcg cacttcactg cgacttcccc gagcaatcac aaacggggta
     1681 tgtaagggcc aggcctcctc aaatacctta acggttctca tctttactcc ttgttgtcgc
     1741 ggtggtctgc ggattaagcg caaatagcgt ttgctgtgtt attgacagtt agcataaact
     1801 aggtgtgacg ttaactatat gtaaacagga aagataatca tgtcacaaac cgttcatttc
     1861 cagggcaacc cggttacagt cgccaattcc atcccgcagg nnnnnnnnnn tattgatacc
     1921 ggtgtttgcg ccgcatcagt acgtaagttt aaccaactgg caaccgagat cgacaacacc
     1981 gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa
     2041 ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaattcct gcaagcctac
     2101 ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt
     2161 gatgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac cgagccggat
     2221 tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg tgttgcacca
     2281 tcaggcatat tcacgcttac tcttcgttct tcttctgact cagaccatat tcccgcaact
     2341 tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc gtgctgggat
     2401 aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt tcgtccagcg
     2461 aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa
     2521 tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcacgatag atagcgttct
     2581 ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc agatcagcgg
     2641 ccagtttcgg acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac agctcggtta
     2701 acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc aacacgttca
     2761 gacgataata gagatcttca cggaacacgc ctttttgcac cagttcgacc agattcttct
     2821 gcgtagcaca aatcacccgc acatcgacat gcacctcatg gtcttcgcca acccgacgga
     2881 aggtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac atctcaccta
     2941 tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaaccctttc ttcccttccg
     3001 gagcatgacc aaacagttca ctctcgaccg catcttccgg tatnnnnnnn nnngacgcac
     3061 agttcagcgc caggtaaggt ttgctcgctc tggggcttgc ctgatgacag gcatacgcaa
     3121 agagatcttt tccggtgccg gtatcacccg taatcagcag tggcgcgctt agcatcgcca
     3181 gtttctgcgc ctgttcgaca acatgcttca ttttcgggct gacagcgaca atttgactga
     3241 aggcgctgac atcctgggcg gcgacatttt gcaactgacg gcccatacga atcgttgatc
     3301 gcagcatcac caccgcaccg gtcaggacgt gttgatcatt ttcatcctga agataaacag
     3361 gcgtaatctc catcaggaaa ttctgcccat taataacgac atgctcgtta tgcgaatctt
     3421 gcggttcgct ttccagccag cgtaaaaaat taaagccgtt aatcaattgc gcggcggtat
     3481 ggttgcgcag gcgatccaat ttttgcccaa aaagctgaca gctcgccggg ttcgccatat
     3541 ccactttgct tttcatatcg acagagagca caggttcagg caacgcctcc agtaacgcgc
     3601 tcaacgccag atgctcacgt tcggaaggca tccacgggac agtacgcaca tcggtaacac
     3661 ctgcaatacg gcgtatttcg gccatcagag tgctgaaact ctcaaactcc agttcagcaa
     3721 aattgaggta gattcgccca atggggtcaa tctcaatacc gcgtaaatca atgcctctt
//
LOCUS       DAWDRC010000392         3767 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9882_length_3767_cov_2.113685, whole genome shotgun sequence.
ACCESSION   DAWDRC010000392 DAWDRC010000000
VERSION     DAWDRC010000392.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9882_length_3767_cov_2.113685"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          905 a          943 c         1044 g          875 t
ORIGIN      
        1 gctgatgccg gaataataat aagcatgatg cggggcgaca gtttgcagat gggtaatcgg
       61 atctggctca tcaccatacg gcaaacgcgc gcccggcgtg gtgccgatat cggaacttat
      121 ccccgactgt gcgtgtaaca tggcgcgttt ttgccagcca tatttcacgg gggtattggc
      181 gacaaactcc gccagttgcg cgctaatgcg ataacccact tcatttgcgg cggcctcttt
      241 accgtagcgt gcggcaatcc cttctttttc acacagcaag ttaaccgctt ccgccagccc
      301 atacatgcca aacatcggca caaaacgttc agggttaatc agcccttctt tcaccaggaa
      361 gctattctca aagaagtgtg attgttgata gaggaattca caccgcgcat cgatgatggc
      421 gatctgctgt tggcagtagt gcggtagagt gcgcgtaaag aagtcctcca gcgattcact
      481 gcgctcggca atggctttca ggtttaggcg taccagcgtg ctgccaccac ccgccagcgg
      541 cagtgagttg taacagctca caatcccgta gccccctttt gtgaaaattt tatcatgcac
      601 cggaccgttg gcgatgtgcg gtttgctaca ttcacagatg tttttcgcca cttccagcag
      661 caggtcatca ggggtgatat caggatcgta gataaaggtc aggttcggtg aaacctgctt
      721 cagctctgcg tctgcacgta atatcgcacg ggtaatgggc gaatcagacg ggccgatatt
      781 ggcatgcata aaggcgtctg acagggttct gtcgaggtaa cgccagaaac gttttattcg
      841 aatatcgatc tcgtcttgtg ttagaattct aacatacggt tgcaacaacg catccagttg
      901 ccccaggtag accggcatcg atgtgaccga aggaacgtgg tggtaaagaa tggtcagcag
      961 agagagtgcg tcatcaagat cttttgcgcc ttccagctcc agccattcgg aaccgttggc
     1021 caaaaaacgg gcgtaatcgg gtaagacata gcgcggtttg tacggcgcat gaccttcaaa
     1081 catatcgcag attaccccct catccagcgc gcggcgggct tcggcaggca gctgtgggta
     1141 aggcagatta ttttctgctt ccagtgccag aaaatggcgc ttctgctccg ggctaagtac
     1201 cgggctggtg acaatttgct ggcaacgttg ttgcagtgca ttttcatgag aagtgggcat
     1261 cttcttttcc ttttatgccg aaggtgatgc gccattgtaa gaagtttcgt gatgttcact
     1321 ttgatcctga tacgtttgcc accactgacg cattcatttg aaagtgaatt atttgaacca
     1381 gatcgcatta cagtgatgca aacttgtaag tagatttcct taattgtgat gtacatcgaa
     1441 gtgtgttgcg gagtagatgt tagaatacta acaaactcgc aaggtgaatt ttattggcga
     1501 caagccagga gaatgaaatg actgatctga aagcaagcag cctgcgtgca ctgaaattga
     1561 tggacctgac caccctgaat gacgacgaca ccgacgagaa agtgatcgcc ctgtgtcatc
     1621 aggccaaaac tccggtcggc aataccgccg ctatctgtat ctatcctcgc tttatcccaa
     1681 ttgctcgcaa aacgctgaaa gagcagggca ccccggaaat ccgtatcgct acggtaacta
     1741 acttcccaca cggtaacgat gacatcgaaa tcgcgctggc agaaacccgt gcggcaatcg
     1801 cctacggtgc cgatgaagtt gacgtagtgt tcccgtaccg tgcgctgatg gcaggtaacg
     1861 agcaggttgg ttttgacctg gtgaaagcct gtaaagaggc ttgcgctgcg gcgaacgtac
     1921 tgctgaaagt gattattgaa accggcgaac tgaaagacga agcgctgatc cgtaaagcgt
     1981 ctgaaatctc catcaaagcg ggtgctgact tcatcaaaac ctctaccggt aaagtggctg
     2041 tgaatgcgac gccggaaagc gcgcgcatca tgatggaagt gatccgcgat atgggcgtag
     2101 aaaaaaccgt tggtttcaaa ccggcgggcg gcgtgcgtac tgcggaagat gcgcagaaat
     2161 atctcgccat tgcagatgaa ctgttcggtg ctgactgggc tgatgcgcgt cactaccgct
     2221 ttggtgcttc cagcttgctg gcaagcctgc tgaaagctct gggccacggc gacggtaaga
     2281 gcgccagcag ctactaaggc ttgatttgcc ggatgatgca gcgatgctca tccggttccc
     2341 cgattacctc gtctcttttc cacgggaggt taccttgttt ctcgcacaag aaattattcg
     2401 taaaaaacgt gatggtcatg cgctgagtga tgaagaaatt cgtttcttta tcaacggcat
     2461 tcgcgacaac actatctccg aagggcagat tgccgccctc gcgatgacca ttttcttcca
     2521 cgatatgaca atgcctgagc gtgtctcgct gaccatggcg atgcgagatt caggaaccgt
     2581 tctcgactgg aaaagcctgc atctgaatgg cccgattgtt gataaacact cgaccggcgg
     2641 tgtcggcgat gtgacttcgc tgatgttggg gccgatggtc gctgcctgtg gcggctatat
     2701 tccgatgatc tccggtcgcg gcctcggtca tactggcggt acgctcgaca aactggaatc
     2761 catccctggc ttcgacattt tcccggatga caaccgtttc cgcgaaatta ttaaagacgt
     2821 cggcgtggcg attatcggcc agaccagctc actggctccg gcggataaac gtttctacgc
     2881 gacccgcgat attaccgcaa ccgtggattc aatcccgctg atcaccgcct ctattctggc
     2941 gaagaaactg gcggaaggtc tggacgcgct ggtgatggac gtgaaagtgg gtagcggcgc
     3001 gtttatgccg acctacgaac tctctgaagc ccttgccgaa gcgattgttg gcgtggctaa
     3061 cggcgctggc gtgcgcacga ccgcgctgct caccgatatg aatcaggtac tggcctccag
     3121 tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc ctgacgggtg aatatcgtaa
     3181 cccgcgtctg tttgatgtca cgatggcact atgcgtggag atgctgatct ccggcaaact
     3241 ggcgaaagat gacgccgaag cgcgcgcgaa attacaggcg gtgctggaca acggtaaagc
     3301 ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc ccgaccgact tcgttgagaa
     3361 ctacgcgaag tatctgccga cagcgatgct gacgaaagca gtctatgctg ataccgaagg
     3421 gtttgtcagt gaaatggata cccgcgcgct ggggatggca gtggttgcaa tgggcggcgg
     3481 acgccgtcag gcatctgaca ccatcgatta cagcgtcggc tttactgata tggcgcgtct
     3541 gggcgaccag gtagacggtc agcgtccgct ggcagttatc cacgcgaaag acgaaaacag
     3601 ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa cttgccgata aagcaccgga
     3661 aagcacacca actgtctatc gccgcatcag cgaataacgg tatactgatc cggtcattta
     3721 aatttgaagc actgagtacg gagaacatat gaaacgtgca tttatta
//
LOCUS       DAWDRC010000393         3760 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9905_length_3760_cov_1.715520, whole genome shotgun sequence.
ACCESSION   DAWDRC010000393 DAWDRC010000000
VERSION     DAWDRC010000393.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9905_length_3760_cov_1.715520"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    383..392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1970..1979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2497..2506
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3039..3048
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          851 a         1031 c          930 g          908 t
ORIGIN      
        1 acggcggacc ggcttcggca gcgatttaat cagggcgatg atcagctcac gacgcagacc
       61 ggggatctgc cactcaaacc cactctcctc aacctggtta agtaacggca gcgggatatg
      121 cacggtcaca ccgtcagcat ccgcgccggg ctcaaactga tagctcaaac gcagcttgag
      181 attgccctga tgccagaagt tcgggtaatc cagcttgctg attttttccg ccccttcttt
      241 gatcaacatg ctcttttcaa agttgagcaa atcaggcgtt tcgcggctga cttttttcca
      301 ccagctgtcg aagtgacgtg cggaaatcac atcgtggctg atgcgctggt cgtagaactc
      361 aaacagcgtt tcgtcatcca ccnnnnnnnn nntgttccag ttcttccact tcggcccgta
      421 gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc accagcgcgt
      481 ggcgaataaa gagttcacga cataacgccg gatcgatctg gctgtagttg accttacgcg
      541 cagcaacaat cggcaaacca taaacagtga ctttttccgt tgccatcacc gcgccctgcg
      601 cccgttccca gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca actggctcca
      661 cccattccgg gtcgatccgc gcagcaatgc gtccccacag gcggctggtt tctaccagtt
      721 ccgccaccat tacccatttc ggcggctttt tgaataaacc agaaccgggg aagatggaga
      781 aacgcgcgtt acgtgcgccg gtatattctt gtttatcggc atctttcatg ccgatatgcg
      841 aaagcaaacc ggtcagcaac gcaatgtgaa tttcgcgata atccgccggt tcgctgttaa
      901 ccggaatgcc aagttctttc accacctggc gcaactgggt gtagatatcc tgccattcgc
      961 gcacgcgcag atagttgaga taatcagtac gacacaggcg acggaaggcg tttgaagaaa
     1021 gcgccttttg ctgctcgcca agataattcc acagattcac aaatgccagg aagtcagact
     1081 ctttgtcgtg gaagcgacga tgtttttcgt cagatgcctg ctgtttatcc atcggacgct
     1141 cacgcgggtc ctgaatggag agcgcggacg tgataatcat cgcctcacgc acgccgccat
     1201 gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc gacaggcaac tgcgagagct
     1261 ggcgaccgag cggcgtcagt ttataggcgc tggcctgttc atccgtggtg atcgcaccca
     1321 gttcttcgag cagacgcacg ccatcctgga tattgcgttt atccggtgct tcgacaaacg
     1381 ggaatgcggc gatatcgccc aagcccagtg cggtcatctg caaaataacc gaggccaggt
     1441 tagtacgcag aatttctggg tcggtaaact ccgggcgcga gaggaaatcg tcttcggaat
     1501 agagacgaat acagatccct tctgacacac gaccgcagcg gcctttacgc tgattagctg
     1561 aagcctgcga aattggctca atcggcaaac gctgcacttt ggtgcgatag ctgtagcggc
     1621 tgatacgcgc cgtacctggg tcgataacgt atttaatacc cggtacggtc agcgaggttt
     1681 ccgcgacgtt ggtcgccagt acaatgcgtc gcccgctgtg cgactggaac acgcggttct
     1741 gttcgctgtt ggaaagccgt gcataaagcg gcaagatttc ggtatggcgt aagttcagct
     1801 tgttcagcgc atcggcggta tcgcgaattt ctcgctcgcc gctcataaag atcagaatgt
     1861 cgccagggct ttcctggctc agttcatcta cggcgtcaaa aatcgcctgc aactggtcgc
     1921 gctcggtgtc atcggcttct tcaacaatcg ggcgatagcg cacttccacn nnnnnnnnnt
     1981 gataataatt tttaggtcag gacgccgcgg cagcaactct ttcaaatagc cgaggagaaa
     2041 atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg atggtgtcgt actgcatcag
     2101 caggcggtct tgctggatct ctgccagcag gataccgtcg gtcatcagct tgaccatcgt
     2161 gttatcactg acgtgatcgc tgaaacgcac tttgtaaccg atgcaaccgc ccggctccgt
     2221 ttttagctct tccgcaatac ggttcgccac cgttcgcgcc gccagccgac gcggctgggt
     2281 atggccgatc agccctttaa tcccgcgacc cagctccata cagattttcg gtagctgagt
     2341 cgttttaccg gaacccgttt ccccggcgac gatcaccacc tggtggtcac gaatcgcttc
     2401 gagaatgtcc tgctttttct gactaaccgg caaattgtcc gggtaagtaa tctccggtcg
     2461 tgccgcttca cgcagcagga ctttccctgc cgcatgnnnn nnnnnncttt cgccatctcc
     2521 tggaaaatgg cctgttgtgc atcaggattt ttaaccttct tcacgccgtg cagacggcga
     2581 gaaaaacgca gtctgtcacg cagcatcagc gaatccagcc gctgctgcaa ggcagtaaag
     2641 gtcaattttt gttgttctgt cataacgtta gtgggcagta gtctgcctga ttaattttct
     2701 tttaaatgat agatatagag taccacatcg cggctttcta tgcgttgttc aataaattcg
     2761 aacataggct tcgatttatt gcgctatctc tgtggcagga ttgtgaataa agtgtcaaca
     2821 agcaacgggg cattgcccat tcaaatatct ataaggaaac accatgagca aggtattagt
     2881 tcttaaatcc agcatcctgg cagggtactc tcagtctaat cagttgtccg attattttgt
     2941 tgaacaatgg cgcgaaaagc actccgctga tgaaatcacc gtgcgtgacc tggctacaaa
     3001 cccgattccg gtcctggatg gcgaactggt tggtgcacnn nnnnnnnnac ttcaacatct
     3061 cgactcagtt gaaaaactat tttgacctgg ttgcacgcgc aggcgttact ttccgctata
     3121 ccgagaaggg tccggaaggc ctggtcaccg gtaaaaaagc catcgttatc accagccgcg
     3181 gtgggatcca taaagatggc ccaacggatc tggtaacgcc atatctgtcc acattcctgg
     3241 gctttatcgg cattaccgat gtgaaatttg tcttcgccga agggatcgcg tacggcccgg
     3301 aaatggcagc gaaagcacag tctgacgcga aagcagccat cgacagtatt gttgctgaat
     3361 aaccggacac gatcttgatg tggactgtag atattcagtc cacatctcaa tccgcttacc
     3421 tgttttccca taatttcagc atcgctttgt gctcttcctt aagcacaatc cgcgagcgtc
     3481 tggctcgaat ataactaatc acttcatcaa cggttttaca atgtccgtaa cataacagcc
     3541 atgccgccac caccagcgca ctgcgcgata atcccaacgc gcaatggacc agaacgctgc
     3601 cttgctcttc gcgtaatgtt tccagcatcg ccacggcctg tcggagctcc ccctcttccg
     3661 gaaccaccag atccaacatc ggtacacaaa aatagagtcg atcttttgtc gctcgtccgc
     3721 gagggaattc aaaggtgacg tccagaaccg cattctgtgc
//
LOCUS       DAWDRC010000394         3756 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9915_length_3756_cov_2.734126, whole genome shotgun sequence.
ACCESSION   DAWDRC010000394 DAWDRC010000000
VERSION     DAWDRC010000394.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9915_length_3756_cov_2.734126"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    294..303
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2921..2930
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          993 a          859 c          831 g         1053 t
ORIGIN      
        1 aagtagtgct aatagattta ataatccata accatttaga ggctattctt aattatttgc
       61 ggtaattctt tattcattcc tcggttatta cgtcatattc agagcaatcc tggtattagt
      121 gtcaccaatt tcttctggcg ataattctga aatgttatga acagttcgag caaactgctt
      181 ttacctgctg cgggttggtg ctagtatgaa aaagtgagtc ctgtcccgct tccttcctaa
      241 ttgtaatttt tcgtaataat gcgatgaaac cctgcaaaaa ggggcgtatg agtnnnnnnn
      301 nnngtggctt atagttaagc taacaaacga gagggcaagt ccaggtcagt aagttttttc
      361 catcccgaaa ggtgtccgtt agttcaaccg ctaagaaggg gacgcgttat ggatgaatac
      421 tcacccaaaa gacatgatat cgcacagctt aagtttctct gtgaaaccct gtatcatgac
      481 tgccttgcaa accttgaaga aagcaatcat ggctgggtta acgacccaac ctcggcgatc
      541 aacctccagt tgaatgaact gattgagcat attgcgacct tcgcacttaa ttacaaaatt
      601 aagtataatg aagacaataa gctcattgag cagatcgacg aatatctgga tgacaccttt
      661 atgttgttca gtagttatgg tattaatatg caggatcttc agaaatggcg gaagtcaggt
      721 aatcgactat tccgttgttt tgtcaatgcg acgaaagaga atcctgcgag tttatcttgt
      781 tagaattatt acaaccatgg gtagaagtat gtccgaaaaa cctttaacga aaaccgatta
      841 tttaatgcgt ttacgtcgtt gccagacaat tgacacgctg gagcgtgtta tcgagaaaaa
      901 taaatacgaa ttatcagata atgaactggc ggtattttac tcagccgcag atcaccgcct
      961 cgccgaattg accatgaata aactgtacga caagatccct tcctcagtat ggaaatttat
     1021 tcgctaataa ataactcact ttcggagcta taactggctg tttattaaga attttgcact
     1081 tttttcgcca tgaagacata ccctatgtga tcttcatcac acagatgtaa tgggaacgtt
     1141 ctcttcactg acttttcgtc ttactgtgtt gccgcatttt cagcaaccgg aagtcagtaa
     1201 tgagcacaga aaaagaaaag atgattgctg gtgagttgta tcactcggca gatgaggcgt
     1261 tatctcgcga tcgcctgcga gctcgtcggc ttattcaccg atacaatcat tccctggcgg
     1321 aagagcacac attacgccag caaattcttg ctgatttatt cggtcaggtg acagaggctt
     1381 atattgagcc aacgtttcgc tgtgactatg gctataacat ttttctcggc aataattttt
     1441 tcgccaactt cgattgcgtg atgcttgatg tctgccctat tcgcattggt gataactgta
     1501 tgttggcacc aggcgttcat atctacacgg caacacatcc catcgaccct gtagcacgta
     1561 atagcggtgc tgaactgggg aaacccgtca ccatcggtaa taacgtctgg attggcggac
     1621 gtgcagtcat taaccctggt gtgaccattg gtgataacgt cgtggtagcc tcaggtgcag
     1681 ttgtcacaaa agatgtcccg gacaacgttg tcgtgggcgg taatccagcc agaatcatta
     1741 aaaaattgta accagctttt ctcaactgtt atgcaaaatc gtggcaaatc tgttacttcc
     1801 cctctactat tcccacgtta aaatagggtg ttccctggaa agttgcagat atcacgaagg
     1861 caaacgatga ccgaaataca acgcctgctg accgaaacga ttgagtctct gaatacccgc
     1921 gaaaaacgcg acaacaaacc ccgctttagt atcagtttta tccgtaaaca tccggggctg
     1981 tttatcggta tgtacgttgc tttttttgcc accctggcgg tgatgttgca gtccgaaacg
     2041 ctttcaggct ctgtctggct actggttgta ttatttatcc tgcttaatgg tttcttcttt
     2101 ttcgatgtct acccacgcta ccgctatgaa gatatcgacg tgctggattt ccgcgtttgc
     2161 tataacggcg aatggtacaa cacgcgcttt gtacctgccg cgctggttga agccatcctg
     2221 aactctccac gtgtcgcgga tgttcataaa gaacaactgc aaaaaatgat cgtccgtaaa
     2281 ggtgaactat ctttttacga tatttttacc ctcgctcgcg ccgaatcaac atcttaagtt
     2341 aggggtacat accaggcgta aagctctacg cctggtcaaa tgacaacgat cgcttccacc
     2401 catcacttca ggaaatacca gctctacctc cttatctcct gccagccttt ttccacaatc
     2461 agatatactt tccctacact gtgttaataa ggatatgctg gtgagaacac gacatctggt
     2521 cagccttatt tcgggagtac tgattctttc agtattgctg cctgtcggct taagcatctg
     2581 gctggcccat cagcaggtag aaacatcatt tattgaagag ctgaatacgt attcctcccg
     2641 cgtcgctatt cgggccaata aggtggcgac acaagggaaa gatgcgctgc aagagctgga
     2701 aagatggcaa ggcgctgcct gtagcgaagc ccatctcatg gaaatgcgtc gggtatctta
     2761 cagttatcgc tatattcagg aagtggttta tatcgataac aacgttcccc agtgttcgtc
     2821 tctggagcat gaaagcccgc ccgatacctt ccccgagcca ggtaaaattt cgaaagatgg
     2881 ttatcgtgtc tggttaacat cgcatagcga tttaggcatt nnnnnnnnnn ttccctatag
     2941 ctcatggcaa attgatgccg ccattattgg caatgcccat aacgttgtca taaccagcag
     3001 cgatgaactt gctcagggaa ttattaccag gctacaaaaa acacccggtg agcatatcga
     3061 aaataatgga atcatttacg atatcctgcc cttaccggag atgaatattt cgatcatcac
     3121 ctgggcttca acgaaaatgt tgcagaaagg ctggcatcgg caagtcttta tttggctacc
     3181 gctcgggttg gtgattggcc tgctggcagc gatgtttgtg ctgcgtattt tgcgccgtat
     3241 ccagtcaccg catcatcggc tgcaggatgc tatcgaaaat cgtgatattt gcgtgcacta
     3301 tcagccgatt gtctccttag ccaatggcaa aattgtcggt gctgaggcac tggcgcgctg
     3361 gccgcagaca gacggtagtt ggttgtcacc agatagtttt attccgctgg cacagcaaac
     3421 gggcctttct gagccattga cgctactgat tataagaagc gtctttgaag atatgggcga
     3481 ctggctgcgt cagcatcctc agcagcatat ttcgatcaat cttgaatcca ccgtgctcac
     3541 ctcggaaaaa atcccgcaat tgctgcgtga aatgatcaat cactatcagg ttaatcccag
     3601 acagatcgcg cttgaactca ctgaacgcga gtttgccgat ccgaaaacca gcgccccgat
     3661 aatttctcgc taccgggagg cgggccatga aatttatctc gatgattttg gtacagggta
     3721 ttcacgttta agctatttac aggatctgga tgtcga
//
LOCUS       DAWDRC010000395         3750 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9932_length_3750_cov_2.497700, whole genome shotgun sequence.
ACCESSION   DAWDRC010000395 DAWDRC010000000
VERSION     DAWDRC010000395.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9932_length_3750_cov_2.497700"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1101 a          870 c          669 g         1110 t
ORIGIN      
        1 atgatgatgg catctatgag tggatgcagc ggcaatggca atagcagcgg cagtccctgc
       61 accttctcca gtgacaatac aacaccccat attacggatt gtcgcgtagg cgaaattatc
      121 tgcatcgatt gcccgtccgc aaaccaacag gttttcaact tgctgcggaa tcattacgcc
      181 ataagggatt tgaaaatagt tcgcttcaag aggtaattta agatgaccat ttccatctat
      241 aaactcaggg aataccccta ttgaatccgc atgacgtccc tcattacaaa tatcattttc
      301 cgtaagctgg actcgcccaa taatatgtct tgattctctt gttcccaccg tcataccgaa
      361 attttttaat tgcgcctgtt caaatcctgg ctggaattga cgcaacgctt caatcgcctg
      421 catggcttgc ttgcgacctt ctatttctgc acgcgtcagg tcaaaaacat ctgtacaatc
      481 aacggcgggg atgctaacaa cattcaaata attcgcatca ccatactcgg tgaccgtact
      541 ccaggaaccg cctaatgtca catcttttgg aataattcct gccgactttc ctttcgcaaa
      601 gactttaccc agataagggc taaacatatc gcgacaagat tcatgaacat cataagacca
      661 gtttttattt tcttcatccg ccccccagtc tccatattta ggttccgtgc tcttaatatt
      721 ttgcatgaac gcatttttat ttatatttgc gcaactaaaa acggttgtca tacacattag
      781 ctcttcgcgt tcacgcttag taaatggtgc tccggcaaac catgcaatat cagcatcgcc
      841 agtacagtca ataatgacat ttgccaaaat agcctgacga ccagatttac tctctgttat
      901 tacgccgagt aaattattgc cctgcttgat aacatcaacg gcggtaatgt gtagtacgcg
      961 gcgcacacct gcctgttcaa gcattgcatc cgcaaccagt ttgaaacgct cggcgttaat
     1021 agcctgacta ttcgattgcg gctcagggct ggacgccccc attgattttg ccgtttcttc
     1081 tatttcgcgt gccagtccac ctgactctac cgtattttca tgacgccacc aggcaattga
     1141 ctcgacgcca gccgttgtca tcattccgcc aaaacaaccg aaacgttcaa tcagcatagt
     1201 agctgcgcct tcacgagcgg cactcattgc tgcggcaata cccgatggac caccaccaac
     1261 aactaacact tccgtggttg tcagcacagg tatattacga gcgggttcca gataaaattc
     1321 catactttat ctccgtattc aataattgag taaaacaatc ccccgcgctt atataagcgc
     1381 gttgatattt ttaattatta acaagcaaca tcatgctaat acagacatac aaggagatca
     1441 tctctctttg cctgtttttt attatttcag gagtgtaaac acattttccg tctccctggc
     1501 taataaccac attggcattt atggagcaca tcacaatatt tcaatgccat taaagcactg
     1561 caccaaaatg aaacactgcg acattaaaat tctttcaatt tgaaaagcat caatctgcat
     1621 gctcaacatt tggatagata ttatagattt taagcttgcg ccataatgtt gtacgaccaa
     1681 tttttaatac ttcagctgct ttagttagat tccattcgca gacacgacat gtatgctcaa
     1741 tagcttgcat ctccaattct gccagtgaag taagatgagg gatatcatcg tcgccactac
     1801 tgttattttg tcggatatcc agaggaatat tctctaaccc gaggacaagt cgattgcagt
     1861 taataaatac cttctccatt cgatttttga actcggagat gttcccaggc caacggtact
     1921 caagcagaac tgaatttgcg tcaggcgtga ttatcatttt ttttcgagtg cgctcctgat
     1981 acttatcaat aatatcgtca atcatttgct tgagatcttc acgacgtttg cgcaatggcg
     2041 gaatttcaat ttcattcatg gagatctcat aatatagctg tcggctaaaa gccccttgtt
     2101 gcacgtactc acgtaaagaa ctactggtac atgtaatcag gcgaaagcgt acggggatca
     2161 aacgatggct gtctgagcga gtaacaagcc ccgttttcaa tactttcaat aaaacactct
     2221 gaacttcccc tgatagatat tcgactttat ccagatataa ggtgccatta tgagccagtt
     2281 caaatttcga tggcgaaggg ccaacatcag agccaagaag ttcgtgtaag atattttctg
     2341 gtgatagcat ctgacaatct acagaaataa atggcccatc acgttgctcg ctctcattat
     2401 gtattgccat cgccaaactc aattttccca caccttcttc gccattgatc aaaatcggag
     2461 agatggattt aattgcccgg cgagcgataa gtaatacctg cttcatacca cctgaagccg
     2521 catgtaaact gtcaaaggta aaatttgcat tagtgcttat ttgctgttgg gctattttcc
     2581 tgattttgtc taatggatga agaaaaagca aaaacgaacc atcgcttaac gggcgaaggg
     2641 taaccataag ttcaataaat tcgccacggc attcgaggac aatttgcttt tgcgatagtt
     2701 tgtttcgttg tgtaattgcc tcattcaaaa tcggcggaaa tacgaatcct tttcggatgt
     2761 tctgaccaat taaactttct ttatcaatat tcagtagtgt ttgtgcttga gaattaacca
     2821 tcgtaattaa agaatgacta ttccaacaaa taaaaccgtc atccatacat tcaatcacag
     2881 cattatgctg gcaaagtact ttgtttatta acttttgttc attctcaata tagatataat
     2941 tagcgatttc tgttgcacat gaatatgatg tttttaattt aacaagctcg gcggtggttt
     3001 caattataca tacaatgtat ccatggatat taccataata atcaatgacc ggagcagcgc
     3061 tcatcgcata acaatgtagc tgttcactaa aatgttctgc tccttccagt tgtgtatata
     3121 aatttgtttc ccgacaaaga ctatatacat tggttccaat gccatcttcg gcccatgaca
     3181 taccagattc aattcctaac tctcgcaatt gactggaata atcgcctgct gcattcaacg
     3241 atagaaccca gccgtgtggg tcaatgatca aaaatgccaa ccggtcgtca ccagccaggg
     3301 tgaaatggtc ttcaacaact ctttgtgaaa ttctaataaa ctcttcgtta cgagaaaaaa
     3361 tcgtctgcaa tgtttgcccg gaagctttga caggcgtgtt ccagtggcga gggtcacaat
     3421 gcttgagaca tttttgccat gacctcagca tcacatgatt agtcgaattt gtcgcgaccc
     3481 aaccatgctt aacaaacatt ttccatttat tttttagatc attatgatac gactctttat
     3541 tcattataat aatatccatt caagtcattt tcagaacttg tatcagaatg ctatgccagt
     3601 aagcttgatc aaacgcaata atatctagcc tggatcatta caaaaacaga ccttatcaac
     3661 aatgtaatca cacatacatt agaaataata accgttaaag caataaaaaa cgaatgataa
     3721 attagataac ttttaaaact gcaaacaaca
//
LOCUS       DAWDRC010000396         3734 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9967_length_3734_cov_2.317478, whole genome shotgun sequence.
ACCESSION   DAWDRC010000396 DAWDRC010000000
VERSION     DAWDRC010000396.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9967_length_3734_cov_2.317478"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          953 a         1003 c          856 g          922 t
ORIGIN      
        1 actgctggcg gcgtatttgt cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct
       61 gtggccgaaa accacctggc tgcgcgtggc gattgccgcc cctgctctct ggcaagtgac
      121 cgagtttctg cgcggttggg tgctgactgg cttcccgtgg ttacagttcg gctatagcca
      181 gattgatggc ccgttaaaag ggctggcacc gataatgggc gtggaagcca ttaacttcct
      241 gctgatgatg gttagtggcc tgctggcact ggcgttggtc aaacgcaact ggcgtccgct
      301 ggtggtgacc gtcgtgctgt ttgcccttcc cttcccgctg cgttacatcc agtggtttac
      361 cccgcaaccg gagaaaacca ttcaggtttc gatggttcag ggcaatattc cgcaatcgct
      421 gaaatgggac gaaggccagc ttcttaatac gctgaagatt tactacaacg caacggcacc
      481 gctgatgggc aaatcatcgc tgattatctg gccggagtcg gcgataaccg atctggaaat
      541 taatcagcaa ccgttcctta aagctctgga cggtgagttg cgcgataaag gtagctcgct
      601 ggtcaccggg attgtcgacg cgcgtctcaa taaacagaac cgctacgata cctacaacac
      661 catcatcaca ctgggtaaag gcgcaccgta cagctatgaa tcagccgatc gctataacaa
      721 aaaccatctg gtgccgtttg gcgagtttgt cccactggag tcgatactgc gtccgttagc
      781 accgttcttt gatttgccga tgtcgtcatt cagccgcgga ccatatattc agccaccgct
      841 gtcgttgaat ggcattcaac ttactgcggc catttgctac gaaatcattc tcggcgagca
      901 agtgcgcgat aacttccgcc cggataccga ctatctgctg actatctcca acgatgcgtg
      961 gtttggtaag tctattggtc catggcagca cttccagatg gcgcgaatgc gtgcgctgga
     1021 gctggcgcgc ccactgttgc gcagcaccaa caacggcatt acggcggtga ttggcccgca
     1081 gggtgagatt caggcgatga tcccgcagtt cacccgcgag gtgttgacca ctaacgtgac
     1141 accaaccacc ggactcacac cgtacgcacg aaccggcaac tggccgctgt gggtgctgac
     1201 ggcgctgttt ggttttgccg ctgtactgat gagtctgcgt cagcgacgta aacgttgatg
     1261 tgaatgatgc gctggcctgc aaaattgcat caattttgca ggccagataa accgttcaca
     1321 ccgcatccgg tatcaacaaa acatactttc tcattgagtt gtggggcatg attatcatgc
     1381 ccttttttct tacggttcag ggatacaagc cacatcgcca tattggggat gccgaataat
     1441 tgcctgcgga tcaagtgaac tttctctgtc caattgtatt agcccgttct tcgccataac
     1501 aaatatcttt ctaccatccg ctgaaaaacg aagattatta acgtagctgg caggataaac
     1561 gccaataatc tgcccggcat taatattgag caggatgaca taaccttgca ggccatatga
     1621 caccaacgct aaatactgac cgtccgggct aataccctca acggaataaa cgctcctgct
     1681 acaccattgc gtcataatgc tggcttgttg atcctcagct cttatacgtg aaatcccctg
     1741 gctatcggca aaaataatct caccattttt tggtcggaat cccatcgcag aaacgacacc
     1801 acgcgcatca cctgcgggaa agtcttcttt ctgatgcgtg atggtattga tccgcacgat
     1861 gttacctctg cctgctcccg gaaccagtaa tgttttttca tccggggaaa gtgccataac
     1921 ccaggcgcct ggtttaccgc tcgtccccgt cccgtcgtat ttctccagtg ggatatccgc
     1981 cacatgctga taagtcacca gatcaatcgc tgacacacta ttactggtgc gattcatcac
     2041 ccaggctttt gtgccatcac gcgagaggat aaggctgtca ggatgggcgc cggtggtaat
     2101 cgactgaata atctcccccg tcgccacgtt aatgaccgat actgcattcg cttcaaatga
     2161 agtcgcataa atcaactttt catccaggct taatgccagc gatctgacgc cctgatcttt
     2221 acagccacca caatgtttga ctgacttttc tttcgctatg ggcgctgtaa aggtatgcag
     2281 gattttcttt tgtccgaggt cgaaaacaac aatgctgttc gtgttttcaa ttgcggcata
     2341 caacgtatta gcatcccgac tgatcgccat atcatggata tcggcaatct cagcgagtaa
     2401 atcaccactg gcctgctcac gacttaccgg agcggataat gtttccctga gggcgttgcg
     2461 ctcctccact aactgctgct ttttttgtcg tcgctcttgt tcaatctcct gcggataaaa
     2521 cgcttcacgc aggctttgcc tgacctgtaa aactgattca tcaatctgct gctgcacaaa
     2581 ccagatagtc ccggtcagaa gaagagtgac cgcaacaata atcccgcctt ccgtaacgag
     2641 ccgattttgc ccaactaaac gcgcactcag ccaacccaac gcgccccccg caatcaggcc
     2701 gccaatgtgg caagcgttat ctattcctgc ttgccgggca ccatacaaca gtgtcagcgc
     2761 caccataccc aacagagaaa atactcgccg ctggttttta tggtgtgtac ccgcacctga
     2821 agcaagctgt gttgctatcg cagcgcccgc tattcccata attgccccgg atgccccaac
     2881 gctgatgact atctgctcac tttcgcgtaa cgcataacag gcgctgatca gcccaccaaa
     2941 gatgccggag aataaccaga taatcaggag tcgccatttc cccagtatcc gttccgccac
     3001 tgcccccact accaggagtg caaacatatt catcagcaaa tgggaaaaac tactgtgcag
     3061 aaaaatactg ctcaataagc gccaggattc atccgacaac gtgagagaag caacgttagc
     3121 gccgagatgg atgagtgcgg attgatcgac ttcttcgtaa ctcatccatt gtgcgaggta
     3181 acaaaatatc ccaacacaga gcgcaaaaag gatcagggtt attgcgggta attgaacatt
     3241 taacggctta accgaagatg cagacataag gtgattcttc cttgaaaaga ctagtaaaaa
     3301 gcattttccc gtgtgtagtt atcatcccgg caaacgtccc acaccgaaaa gttaacactg
     3361 tccgaaatat gactttttca aactgaaatt acaatcgtaa ttaattcacc cctaagtatt
     3421 ttttggcatg taagttgctt tatttactgt gtaagcaatg aaatcagccg caacgcaacc
     3481 gggttgcacc accacaaatc agcggtagca ccaaaaaggt gcggtttaag aattttgctt
     3541 cggaatggtg cggcgcacct cgcaaaaata aacaataaaa catcaatatc tttacattta
     3601 tccaaactaa atgttaccta acaaatacaa cactgcacaa taaagtcgca gatgataaca
     3661 acacaaacac tcacaacggg tatccatgcg tttttaacgt agaagataaa ggaattggat
     3721 atgcaattac gtaa
//
LOCUS       DAWDRC010000397         3731 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_9979_length_3731_cov_2.379489, whole genome shotgun sequence.
ACCESSION   DAWDRC010000397 DAWDRC010000000
VERSION     DAWDRC010000397.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9979_length_3731_cov_2.379489"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1216..1225
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3149..3158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          780 a          945 c          958 g         1028 t
ORIGIN      
        1 gcgtggatgg ccttcgccca gaccgatatc aaacgtctga tcgcttacac ctcggtttcc
       61 cacatgggct tcgtgctgat tgctatctac accggcagcc agttggccta ccagggcgcg
      121 gtaatccaga tgattgcgca cggcttgtcg gcggcgggtc tgtttattct ttgtggtcag
      181 ctttatgaac gtattcacac ccgcgacatg cgcatgatgg gcggtctgtg gagcaagatg
      241 aaatggctgc cagcactgtc gctgttcttt gcggtggcaa cgcttgggat gcctggcacc
      301 ggtaacttcg tcggcgaatt tatgattctg ttcggcagct tccaggttgt cccggtgatt
      361 accgttatct ctacctttgg gctggtcttt gcttctgttt attcgctggc gatgctgcat
      421 cgcgcttatt tcggtaaagc gaaaagccag attgccagcc aggaactgcc agggatgtcg
      481 ctgcgtgagc tgtttatgat cctgttgctg gtggtgctgc tggtactgct gggcttctat
      541 ccgcagccga ttctggatac ctcgcactcc gcgattggca atatccagca gtggtttgtt
      601 aattccgtta ctactacaag gccgtaaatc gccatgacaa taactccaca aaacctgatc
      661 gcactgctac cgttgctgat cgtcggcttg acggtggtgg ttgtgatgct ctccattgcg
      721 tggcgacgca atcatttcct caacgctacg ctctcggtta ttgggcttaa cgcggcgctg
      781 gtttcgctct ggtttgtcgg ccaggcgggc gctatggacg ttacgccgct gatgcgcgtt
      841 gatggtttcg ccatgcttta caccgggctg gtattgttgg cgagcctcgc cacctgtact
      901 ttcgcctacc cgtggcttga aggctataac gacaacaagg atgagttcta cctgttggtg
      961 ttaattgccg cgctgggcgg gatcctgctg gcgaatgcca accatctggc gtctctgttc
     1021 ctcggtatcg aactgatctc tttgccgctg tttggcctgg tcggttacgc tttccgccag
     1081 aaacgttcgc tggaagccag tatcaaatac accatccttt ctgccgcagc gtcttctttc
     1141 ctgctgttcg gtatggcgct ggtgtatgcg cagtctggcg acctgtcgtt tgtcgcactg
     1201 ggtaaaaacc tcggcnnnnn nnnnnccgct gttgctggca ggtttcggcc tgatgattgt
     1261 tggcctcggc ttcaaactct ctctggtgcc gttccacctg tggacgccag acgtatacca
     1321 gggcgcgcct gcgccggttt ccactttcct ggcgacggcg agcaaaatcg ctatcttcgg
     1381 cgtggtgatg cgtttgttcc tttacgcacc ggtgggtgac agtgaagcga ttcgcgtagt
     1441 gctggcgatt atcgcctttg cctccatcat cttcggtaac ctgatggcgc tgagccagac
     1501 caatatcaaa cgtctgctcg gttactcatc tatctctcac ctcggctatc tgctggtggc
     1561 gctgattgcg ctgcaaaccg gcgagatgtc gatggaagcg gtaggggttt acctggtcgg
     1621 ttatctgttc agcagcctcg gcgcgttcgg cgtggtcagc ctgatgtcca gcccgtatcg
     1681 tggcccggat gctgattccc tgttctctta ccgcggtctg ttctggcatc gtccgatcct
     1741 cgcggcagtg atgacggtga tgatgctttc actggctggt atcccaatga cgctgggctt
     1801 tatcggtaag ttctacgtgc tggcggtcgg tgtccaggca cacttgtggt ggctggtggg
     1861 tgccgtggtt gttggttcgg caatcggcct ttactactac ctgcgcgtgg cggtgagctt
     1921 atacctgcac gctccggaac aaccgggtcg cgatgcacca tcaaactggc agtacagcgc
     1981 aggcggtatc gtggtgctga tctctgcact gttggtactg gtgctgggtg tatggccaca
     2041 accgctgatt agcattgtgc ggttagcaat gccgctgatg taaacagtca gtttgtaagt
     2101 cagaaagccg ccgacatgtt cggcggtttt ttttggtgcc ctgacaccat cacccaacaa
     2161 taaccgtcag cgcatgattc ccttgtcgca gcatacgtcg atacgcgcta atcagctttt
     2221 ccatatcaag catgatcgaa gcaaagagtt gcccctctaa ctcttctgaa aaggttactc
     2281 ccggataaat ctccatttca ttgagtcgct taatggaaaa ctgtttacgc agctcacttc
     2341 gatccagtga ttccagtcgg tcgattgtcg cggctaactc ctgattccag gtcaatccca
     2401 ggaagccatc tatgccatct tctaggctgt gttcaccaag cacggcgcga ctcagcgggt
     2461 cattcttcgc gggatcaaaa gcagaggtgc ctgtcagccc aaagtgtaaa aaatcccagc
     2521 gtttatcaat atccagtcgg cgtaatcctg aaagcgtgtc atgtatatta tccatcaaag
     2581 gttttttagt agactcaagc agttggttaa tcttttctga gtcaatttct gcaaaatagc
     2641 caatcattcc catttatttt tcctccgttg aatttcatgt ttcgcatctt aaaacagacg
     2701 aaagaaaata aaatgcgcat gttgcttttt ctggatccgc ctcgagaaat ttttattatt
     2761 tactgttgta taacaaggtt gtaactccat ccactacctg agccattttc tgataattta
     2821 acctgtctgc gatatcaccg ggcaagtggt attgtttatt acgataaaaa gcggtgtcag
     2881 taatcattac ggcgggaata tcgtgttgcc aataaataag gtgatcagag aaatcaatac
     2941 cgggaataaa acctggggca ttcatagaat aaacagataa atcccgggat gacaacaatg
     3001 ccgcttttac gtgacgaacg gcgttgatat cctgcattct gccgaccacg gcaataaagt
     3061 ctccccgatc gggataaagc caggacatgg ccggggatgg gtaatcctgg ctaccaggcg
     3121 ttgagtcgta ataaccaatc atctccagnn nnnnnnnnca ttaatttcac tggacgctca
     3181 agcgaagctg catgtaccgc gctccccatt tcatcgctac gaaagaaggg cggttcttcc
     3241 gacgcatagg cgaccagttg gacgccaatc cctggagtct gctgctgtaa caaacgtgcc
     3301 agttcgagta atcctgccac accgctggcg ttatcatccg cgcccggcgt ataggtcaat
     3361 tgatcgtttt cataactact gacagagtca tagtgcgcac caataataat cagcggtcca
     3421 tcggcaggac cataattagc aacaatgttt ttgtaggggc cacccgtaat ggggacatcc
     3481 tgcgcggtaa ctctggcacc gttactgata aagacctctt ttatatactc tgcggaccta
     3541 ttcagattat caatattgtc ggcgctacgt ggatgcactg tttgtgtaag ataacgaact
     3601 gttttttcta attgctcagg acttgcgtgt cggggcgttg gcggtaatgc attcacccag
     3661 ggttggtaaa aaataatcag agggagtgaa aacagaaata atataataaa agcaaagttt
     3721 atttttttca t
//
LOCUS       DAWDRC010000398         3718 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10016_length_3718_cov_2.330876, whole genome shotgun sequence.
ACCESSION   DAWDRC010000398 DAWDRC010000000
VERSION     DAWDRC010000398.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10016_length_3718_cov_2.330876"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1684..1693
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          965 a          886 c          963 g          894 t
ORIGIN      
        1 taccccttaa ggggtatatt gctgatttgt ataaattttc ttaagtcacg gaattgtctg
       61 acatttcatg tttgtgcaaa aaacagacaa attgaacgga tttatagaaa tagatgtacg
      121 tagcagagta agagaggaaa aggccggacg acgatagtgc cgcccggcat gaacaattaa
      181 tgagaggtca gggtgtgacg gtgacggctg acgaaaccta acaggaagca catcacaaag
      241 acgacagcgt acagaccgtt tgccgtcagc agcgccgcct gcggaccact atgttcaaca
      301 atcgggccgg taaccacaaa ggtcaacata gtaccgatgg tcccgcaggt caggacaaag
      361 ttaaccagtt ttggcgacgg tactttggtc tgctgtgaac ccagggtgat gatggtggta
      421 tagatcgcgc tggagaagaa gcccagggcg agaattgacc acgccatatg tgccggtgtt
      481 ccggtgttaa agacgtacat cagaatcgca gccagaccag ccagtacggt caggatacgt
      541 tgcaaatcaa agaagcgaag aataaagctg aatgcccaca tgccgaccat gtacgacatc
      601 cagaagttac ttaccagcgt gccagcgtca ttcaggctca tacccagacc tttggcatat
      661 tctggcaccc aagagataaa acctaactga ccgaggatgt agcacagcgc cgcaacagag
      721 agaaacagta cgccgatccc ccacttttct ttctctaccg gagcatccgt ttttggcgca
      781 tgtttgccca gcgccgggaa ctcacagccg aaggtcagaa taaaaatagc gacatatacc
      841 agcccgatgc aggcataaac ccagtaccac tcaatgctac gtgccagcag aaacgcggcg
      901 atcattggga aaatcatccc ggccatactg aagaaggagt cggtaaataa caggcgtgaa
      961 ccgcgctgac gtccttcata catttgtgtt actaggaatg taccaatcga catggtgatg
     1021 ccgctgacca ccccgagaat gaacatcgcc gccgagaaca gcgccaggct gtggctgaac
     1081 atcaaaccgg caaccgccag caccatcagg agcaagccaa aacgtaactg cgttttcaac
     1141 gggacgattt ccatcagcca ggcgttgagg aagatagaga ttaaaatgcc ggcattgagg
     1201 aaggtgaagg tgttactcat gctggaaaca ggcagattga aataatcggc gatatttccc
     1261 atcaccatcc cggtgacaat aaccaacgca ccggtcagtg cgtaggaaag aaagctaatc
     1321 catgtgagct tgatgcgatt gctgttagtc atgattggcc tgcgttcaaa aataaaatgg
     1381 catagcgggc tatgccgcga gcgggcgatt ttaggcgatt ttgtgatctg attaaatgtt
     1441 ttattgcaat cggttgctaa attgcatttt aagaggtgat tttgatcacg gaataaaaag
     1501 ttatcctcag gttacatata tttcagatac gtaaaattag gtaaagggat ggccttgttc
     1561 ttgaaggcta tttagaatct cttcacttgc tttttttctg ctctgtttgt taaggaaatc
     1621 tcatgttcaa atcgaccctg gcggcgatgg ctgctgtttt cgctctttct gctctttctc
     1681 ccgnnnnnnn nnngttgaca acctcagctg gtaacatcga actggagctg gataaacaaa
     1741 aagcgccagt gtctgtgcaa aactttgtcg attatgtgaa cagtggtttt tataacaaca
     1801 cgacctttca ccgcgtcatt cctggcttta tgattcaggg cggcggtttc accgagcaga
     1861 tgcagcagaa aaaaccaaac ccgccaatca aaaatgaagc agataacggc ctgcgcaaca
     1921 cgcgtggcac catcgcgatg gcgcgtaccg ctgacaaaga cagcgccacc agccagttct
     1981 ttatcaacgt tgccgataac gccttccttg accacggtca gcgtgatttc ggttacgcgg
     2041 tatttggtaa agtggtgaaa ggcatggacg ttgccgataa gatttcccag gtgccgactc
     2101 atgacgttgg tccgtaccag aatgtgccgt caaaaccggt agttatcctt tccgctaaag
     2161 tcctgccgta atgatttctc gcgcgggcaa tcttgcccgc gcttctgctc tcccggcgta
     2221 acccggattt gccgcttata cttgtggcaa atggacacgt tcagggaggc atcaagtgaa
     2281 gaaactcacc gataagcaaa agtcccgtct ctgggagctt cagcgtaatc gtaatttcca
     2341 ggccagtcgc cgtcttgaag gcgtcgagat gcctttagtc actcttactg ccgcagaggc
     2401 tttagcgcgc cttgaagcgc tgaggaggca ctatgagcga taaattcggc gaagggcgtg
     2461 acccatatct ctaccctggt ctcgatatca tgcgtaataa gctcaacatc cgccagcagc
     2521 agcggctgga acaggccgct tacgaaatga cggcgctgcg tgctgcgacc attgaacttg
     2581 gcccgttggt tcggggttta ccgcatttgc gtactcttca tcgccagttg tatcaggata
     2641 ttttcgactg ggcggggcaa ctgcgcgaag tcgatattta tcagggcgat actccgttct
     2701 gccactttgc ttatatcgaa aaagagggca atgccctgat gcaggatctg gaggaagaag
     2761 gttatctggt tggcctggag aaagcgaagt tcgtcgagcg gctggcgcat tactattgtg
     2821 aaatcaacgt gctgcatccc ttccgggtgg gaagtggttt ggcacagcgg atcttcttcg
     2881 agcaactggc gattcatgcc ggatatcaac tgagctggca gggtattgaa aaagaggcct
     2941 ggaatcaggc aaatcagaat ggggcaatgg gggatctcac cgcgctgcag atgatattta
     3001 gcaaagtggt aagcgaagcc ggggaatctg agtaaaatag cgcggttctt tcgtaccgga
     3061 gccgccatga tcctgcttat tgataactac gattctttta cctggaacct ctaccagtac
     3121 ttttgtgaac tgggggcgga tgtgttggtt aagcgcaacg atgcgttgac gttggcggat
     3181 atcgacgccc ttaaaccaca aaaaattgtc atctcacccg gcccctgtac gccagatgaa
     3241 gccgggatct cccttgacgt tattcgtcac tatgccggac gcttgccgat tcttggcgtc
     3301 tgcctcggtc atcaggcaat ggcgcaggca tttggcggca aagttgtgcg cgccgcaaag
     3361 gtcatgcacg gcaaaacctc gccgattaca cataatggtg tgggcgtatt taaggggctg
     3421 gcaaatccac tcaccgtgac acgctaccat tcgctggtgg tggaacctga ctcattaccg
     3481 gagtgctttg aagtgacggc ctggagcgaa acccgcgaga ttatggggat tcgccatcgc
     3541 cagtgggatc tggaaggtgt gcagttccat ccagaaagta ttcttagcga acaaggacat
     3601 caactgctgg ctaattttct gcatcgctga tttctgattg ccattcagtg attttttatg
     3661 catattttgt gattataatt tcatatttat ttatttattt atgcgtaaca gggtgatc
//
LOCUS       DAWDRC010000399         3688 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10124_length_3688_cov_2.227360, whole genome shotgun sequence.
ACCESSION   DAWDRC010000399 DAWDRC010000000
VERSION     DAWDRC010000399.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10124_length_3688_cov_2.227360"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1669..1678
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          760 a          942 c         1027 g          949 t
ORIGIN      
        1 gcttggcgtg aacgccggtg ccagttttgc cattgtgctg ggcgcggcgc tgtttggtta
       61 ctcttccgcg caggagcaac tggcgatggc cttcgccggg gcgctggtgg cctcattgat
      121 tgttgccttt accggcagtc agggcggcgg gcagttaagt ccggtgcgtt taacccttgc
      181 aggcgtggcg ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc tgcttaaccc
      241 tgacgtctac gatcagttgc gtttctggca agccggttca ctggatattc gcaatctaca
      301 taccttaaaa gtggtgctga tcccggtgct taccgctgga gcaaccgcgc tattactgag
      361 ccgcgcgctg aacagtttaa gccttggcag tgacaccgcg acggcgctgg gcagtcgcgt
      421 agcgcgcaca cagttgattg gtctgctggc aattaccgtg ctttgtggta gtgcgacggc
      481 ggtagttggc ccgattgcct ttattggcct gatgatgccg cacatggcgc gctggctggt
      541 aggtgccgat catcgctggt cgctgcccgt cacgctactc gctagctctg ccctgctgct
      601 gtttgccgat atcattgggc gggtgattgt tcccggcgaa ctgcgcgttt ctgtagtcag
      661 tgcctttatt ggcgcaccag tgctgatctt cctcgtacga cgtaaaacgc gaggtggcgc
      721 atgatttacg tctctcgccg attaattatc acctgtctgc tgttgctaat agcttgtgtg
      781 atggcaggcg tgtggggatt acgtagcggt gccgtcacgc tggaaacttc gcaggtattc
      841 gccgcgttga tgggcgacac gccgcgcagt atgacgatgg tggtcaccga atggcgttta
      901 ccacgcgtgc tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg cgcgattttt
      961 cagtcgctga tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa caccggggcg
     1021 tggagcggcg tgctggtggc gatggtgctg tttggtcagg acctgacggt tatcgcgctg
     1081 gcagcaatgg tgggcggcat tgtcacttcg ctgttggtct ggctgctcgc ctggcgtaac
     1141 ggtatcgaca cctttcggtt gattattatc ggtatcggtg ttcgcgccat gctggtggcc
     1201 tttaatacct ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg
     1261 aatgccggat cgctcaatgg cctgacgtgg gcaaaaacct cgccttccgc acccatcatt
     1321 atattaatgt taattgccgc cgccttgctg gtgcggcgaa tgcgcttgct ggaaatgggc
     1381 gatgataccg catgtgcgct gggcgtcagc gtcgaacgtt cgcgactgtt aatgatgctg
     1441 gtcgccgtag tgcttaccgc agctgccact gccctggcgg ggccaatttc ctttattgct
     1501 ttagtcgcgc cacacattgc ccgccgcatc agtggcaccg ctcgctgggg gctgacccag
     1561 gcggcgctgt gcggggcgtt gttactgctg gtggccgatc tctgcgccca acaactgttt
     1621 atgccgtatc aacttccggt tggcgttgtt cccgtcagcc ctggcgggnn nnnnnnnnct
     1681 tatcgtcttg ttaattcagg agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc
     1741 ggcgaacagt taaccctcgg atatggcaaa tataccgttg cggaaaatct gactgtagaa
     1801 atacctgatg gtcacttcac ggcaattatc gggccaaatg gctgcggtaa atccacgtta
     1861 ctgcgtacct taagccgcct gatgacgcct gctcacgggc atgtctggct ggatggcgag
     1921 cacattcaac attacgccag taaagaggtt gcacgccgga ttggtctgtt ggcgcaaaat
     1981 gctaccacac cgggcgatat caccgtgcag gagctggtgg cgcgtgggcg ttacccgcat
     2041 caaccgctgt ttacccgctg gcgcaaagaa gatgaagacg cggtaacaaa agcgatgcag
     2101 gccacgggaa taactcatct ggcggatcaa agcgtggaca ccctttctgg cggacaacgc
     2161 cagcgagcgt ggatcgcgat ggtgctggcc caggaaacgg caattatgct gctcgacgaa
     2221 cccacgacct ggctggatat cagtcatcag attgatttgc tggaactgtt aagcgaactg
     2281 aaccgcgaga aaggctatac cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt
     2341 tacgccagcc atttgattgc attgcgggaa gggaaaattg ttgctcaggg agcaccgaag
     2401 gagattgtca ctgctgaact gatcgagcgc atttatggtc tgcgctgcat gattattgac
     2461 gatccggttg ccggaacgcc gctcgtggtg ccgctcggac gaacggcacc ttcaaccgca
     2521 aagatttaaa ctaagtggtc tgccatcatg gcatcctgtt ttctggatgc catcgcatga
     2581 cgcaacagca caccaccaca agccaccatc ccgccgatca acgcggaaag gatcacaata
     2641 atagccttac ccggaccgtc ttttttcact ggcaatgacg gtcttaactg atatttaaac
     2701 ggcgtaaaat tcacatcgtt gatatttgct tttgttaact gctcaacaag atactggcga
     2761 ttacgtaatt cgccgttcag ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg
     2821 cgttcaatac cgtctgcgcc gagagaaata gagaaatcgg ggtcatcttt aacggcctga
     2881 ccattactgt atacgggctt tttaattcct gccgcgttgg caatgtcgag tgaataattg
     2941 aggcgctgaa tgtttgcatc aagttgattt ttcattttaa tgcggtcctg agccagtttt
     3001 gctttttcaa actgggtttt gatctccagt ttattacgga cgttttctat cgactctttc
     3061 accaccaaca cagagatata atcgatatac ccgctcaaaa cggtctgcgc ctcttcactg
     3121 gttggcgcgg taaaacttag cgtccaggag gtatacagtg acggttcatc ttttttctta
     3181 ctggcattgt catcaaccgc tttcattttt tcgctcaatg cgacaattgc gcgatgcaaa
     3241 tccagttcgt cgattttcgc ctcttttaat tggtccatta cataaggtga tgagcgcagg
     3301 tactcttcta gtaagctaac agactgaaac ttcttgataa acaggttaaa tgcaccgcca
     3361 cggtcgatat taacatcgag atcaagaacg cggagtttgg taaaggtttt ctccaggtcc
     3421 tgccactgaa tggcttcggc gggagtaatg acggctgagc tggtccattt ttgcggcagg
     3481 atgaaagaga tcagcaagcc tgcgcaggca aacgcaaaaa cgaccgccat gaccgttttt
     3541 ttggcccgcc ataaaacttc aattagacta agtaaatcaa tttcattatt actgggcgac
     3601 gccagaggat actcgggaaa atgagcttca cttccctgtt taatattcag tgatgacatg
     3661 tagtgaaacc tgaggtttta gtccgtaa
//
LOCUS       DAWDRC010000400         3682 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10142_length_3682_cov_2.424318, whole genome shotgun sequence.
ACCESSION   DAWDRC010000400 DAWDRC010000000
VERSION     DAWDRC010000400.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10142_length_3682_cov_2.424318"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    369..378
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          853 a         1042 c          940 g          837 t
ORIGIN      
        1 cgatggactg ttcatttgct gcgccagttc gcccagctgc gtgagcgtac cgtcaatcac
       61 gcgctgcgtg actgccgttc cgttttcgac aattgccacc ggcatttcac caggcatacc
      121 gtattcaatc agcttttgct gaatagttgc agcctgattc agccccatat aaaacaccag
      181 cgtctgtttt tctgccgcca ggttttccca gtccagctcg ccaccggttt ttaagtgtcc
      241 agtaattaag cgtacgctct gggcataatc gcgatgcgtg agcggaatac ccgaataggc
      301 agagcaacca gaagctgcgg taatacccgg aaccaccgag aatggaatac ccgcgttgca
      361 cagtgtttnn nnnnnnnncc tttttgcgct tcccgcagca ggatctggtt aatctcttcc
      421 tgcggtacgc agtggtatcc cgcgcgtttg ccgacgaaaa cgcgatcagc atcgcggcgt
      481 accagattca taatatcgtc agaaaccaga cggtcgtaga ccaccacatc tgcctgctga
      541 atttgttgca gacctttcag tgtcagcagc ccggcatcgc ccggacctgc accaaccagc
      601 accacttcac cgcgatggtc gggcggttca ttgattaact gttcggtggt ttcagtaatg
      661 gctttctgat cgttgttggc cagcgactgc gccaggcggt cgttaacgaa caatttctcc
      721 cagaaacggc gacgctcacc catcgtggcg aactgttgtt tcactcgccc gcgtaattgc
      781 ccggcgtatt tcgctacctg gcccagatgt aacggcagca gtgattcgag tttttcgcgc
      841 aacaggcgcg ccagaaccgg agaggtgccg ccagaggaga ccgctaccat gagcggcgag
      901 cggtcaataa tcgacggcat aataaagctg gcggctttcg gcgcatcgac cacgttacag
      961 aagatgcgac gagcttcagc ggcttcgctg acgcgctggt taagcgcgtc atcatctgtc
     1021 gctgcaatcg ccagccagca ggtgtcgaga aggctttcat caaatggccc ttcgacgagg
     1081 gttaacatgc ctgcatctgc ccatgcggtg aactgtggaa taaacgctaa tgcattcacc
     1141 gttaagcgag tgcctgcgtc taacagcaac cttgctttgc gttccgcgac atcaccaccg
     1201 ccgacaatca gacagtcgcg atcgcgtaat tggcaaaata taggcaaatg atccacgtaa
     1261 aaacccctta gtaattaacc ggcagccgtt tcagtttgat taaatttgtc cgcaaccgga
     1321 cgattcgctt tcggcgtagc ataccaataa cccaatccca tgaatacggc acccgataaa
     1381 gtattaccca gcgtcaccca cagcaggtta tgaccaatgc ccgccagcgt gtaggcttcg
     1441 ctgtggttgc cgaaccagga gagtgcgaac agcgtcatgt tagcgatgga gtgctcgtag
     1501 ccggacgcga taaatgccag caaacaccac cagatagcga taaatttcgc cgccccttca
     1561 gtgcgcagcg ccatccagat tgccaggcaa accagccagt tacacaatgc acctttgaag
     1621 aacagtacca ttgccggtgc agtggtttta gccagcgcaa cggagtgaac gatgctggta
     1681 tctaccggca gcaggctacc gccgccccag ctataaagca tggcaacgaa gacggaaccg
     1741 accaggttac ccagccaggt ttgcggcagg attgcccaca tttgcccgtg gctgatggtg
     1801 cccgctttca ccccaaaggt gaggaacatg gtgtgtccgg tgaacaattc agaaccggcg
     1861 ataatcacca gcgttaaggc gataccaaag gtcgcgccca tcaccagagg acgtacggac
     1921 gggtcgagca aattaccgag cgtgaaaatc aggatgatcc caagacccac atacgcgccc
     1981 gccatcgcgg agctgaccca aaagccgagc gggttatttg ccgacaggcg tgcaatgcgc
     2041 gcagcgttag ccgcacactt agtaatagtg tctgtaaaca tttgattatc ctttaaaaaa
     2101 taaaaacaaa aaataaaaca aaaagtttaa aaaatgtcct gcaatatacc gaaggttatt
     2161 acaggaccat gcaaaaaggg gaggcattgc gcctcccgtt aaaatattaa ccgcgcagct
     2221 gtaccacgcc gtctttcact cgcgcgtcgt aatgcttgac ggaaaactgt tcgtcttcca
     2281 tgcacaagcc gtcgcttaag cgaaaacgct gctttttcag cggactggcg acccacagct
     2341 cgccctggtg ttccgcaatc agcccgcgtg acagcacgct ggactcgaag aacgggtcga
     2401 tgttgctgat cgcaaacacc tgatcgctgt gatacgggcg gaaaattgct acttgttcgt
     2461 cacctaacag cgcgcagacg ccggtttcag gcaggatgtc atcgattttg cagatgtctt
     2521 tccactggct catgcgttgt cctccaccag agttaccggg atacgttcat acggcgttgc
     2581 cggacggtgc tgttcgcgct ctggcaccat ctgcacgttc gggtcacgct tgtcgctgtt
     2641 gatgaagtgt ttgaagcgag tctgcgcaga cggcgtattg accgtttcag tccattcgca
     2701 cactaccgct tcccgcaggc gcgccatctc ttcttccaga tgagcgttca gccccagttt
     2761 gtcgtcaatg atcaccgctt tcaggtaatc gatgccacct tcgaggtttt ctaaccacgg
     2821 cgcggtacgc gtcagtttgt cggcagtacg gatgtagaac atcatgaagc ggtcgagata
     2881 tttgatcagc gtttcgcgat cgatatccgc tgccagcaga tccgcatgac gcggtttcat
     2941 gccgccgtta ccgcaaacgt acaggttcca gcctttttcg gtggcgataa tacccacgtc
     3001 tttaccctga gcttctgaac attcacgggt acagccggag acaccgaact tcattttgtg
     3061 cggcgtacgg atgcctttgt aacggttttc cagttccacg ccgaggccga cgctgtcgcc
     3121 aacaccgtag cggcaccagg tgcttcccac gcaggttttc gccatacgca gtgctttcgc
     3181 ataggcatga ccggtttcga agccggcttc aatcagttga cgccagatct ctggcagatc
     3241 gtctttctgt gcgccaaaca tcgccaggcg ctgagagccg gtgattttgg tgtagagatt
     3301 aaattcacgc gcgatacgac ccactgccat cagcccttcc ggggtgattt caccgcccgg
     3361 agaacgcggg atcaccgagt aggtgccgtc tttctggatg ttcgcgagga agttgtcgtt
     3421 agaatcctgc agcggagtat gttccggctt cagaatgtat tcgttccagc aggaggccaa
     3481 cagcgaaccg acggtcggtt tacaaacttc acaaccgtaa cctttgccgt gtttcgccag
     3541 cagttcttcg aaggttttaa tgccttcaac gcggatcaaa tggaacagtt cctgacgcga
     3601 ataagcaaag tgttcacaca ggttgttgtt aacttcgata ccctgtttcg ccagttccgc
     3661 gttcagtacc tgagtgacca gc
//
LOCUS       DAWDRC010000401         3677 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10159_length_3677_cov_2.447543, whole genome shotgun sequence.
ACCESSION   DAWDRC010000401 DAWDRC010000000
VERSION     DAWDRC010000401.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10159_length_3677_cov_2.447543"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    338..347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          898 a          930 c         1015 g          824 t
ORIGIN      
        1 gaacaacgca ccgtcggcat tggtcgtctg gacccggaaa tcgcccgcga cttcagtaac
       61 gttggcccga tggttcgtgc cagcggtcac gcccgtgata cccgtgccga tcacccgttt
      121 gttggttatg gcctgctgcc aatggaagtc cacagcgagc agggctgcga cgttatttcc
      181 cgtctgaaag tgcgtatcaa cgaagtctat accgcgctga atatgatcga ttacggtctg
      241 gataacctgc cgggcggccc gctgatggtg gaaggcttta cctacattcc gcaccgtttc
      301 gcgctgggct ttgccgaagc gccgcgcggc gacgatannn nnnnnnncga cgatatccac
      361 tggagcatga ccggcgacaa ccagaagctg taccgctggc gctgccgtgc ggcgacctac
      421 gcgaactggc cgaccctgcg ctacatgctg cgcggcaaca ccgtttccga tgcgccgctg
      481 attatcggta gcctggaccc ttgctactcc tgtaccgacc gcatgaccgt ggtcgatgta
      541 cgtaagaaga agagcaaagt ggtgccgtac aaagaacttg agcgctacag cattgagcgt
      601 aaaaactcgc cgctgaaata aggaatcgcc atgtttacct ttatcaaaaa agtcatcaaa
      661 accggcacgg cgacttcgtc ttatccgctg gagccgattg cggttgataa aaacttccgt
      721 ggtaagccag agcagaaccc gcagcagtgc attggctgtg cggcttgcgt caatgcctgc
      781 ccgtcaaacg ccttaacggt tgaaaccgac ctcgccactg gtgagctggc gtggcagttt
      841 gaccttgggc gctgcatctt ctgtgggcgc tgcgaagaag tctgcccgac agcggcgatc
      901 aaactgtcgc aagagtacga actggcggtg tggaagaaag aagatttcct gcaacagtcc
      961 cgcttcgcgc tgtgcaactg ccgcgtctgc aatcgtcctt tcgccgtcca gaaagagatc
     1021 gactacgcca ttgcgctgct taagcacaac ggtgacagcc gcgcggaaaa ccaccgcgaa
     1081 agctttgaga cttgcccgga atgtaagcgc cagaaatgcc tggtgccgtc cgaccgtatt
     1141 gaactgactc gccatatgaa agaggccatc tgatgagtaa tttattaggc ccccgtgacg
     1201 ccaacggcat tccggtcccg atgacggtgg atgaatccat cgccagcatg aaggcgtcgt
     1261 tactgaaaaa aatcaaacgt tctgcctacg tttatcgcgt ggactgcggc ggctgtaacg
     1321 gttgcgaaat cgaaattttc gccacgcttt cgccgctgtt cgatgcagaa cgcttcggca
     1381 ttaaagtcgt tccgtcaccg cgtcatgcgg atattttact gtttaccggc gcggtcaccc
     1441 gtgcaatgcg atcccctacg ctgcgtgcgt ggcagtccgc gccggacccg aaaatctgta
     1501 tctcctacgg tgcctgcggt aacagtggcg ggatcttcca cgatctctac tgcgtgtggg
     1561 gcggtacgga taaaatcgtt ccagtggatg tttatatccc tggctgcccg ccaacgcctg
     1621 ccgccacgct gtacggcttt gcgatggcgc tcggcctgct ggagcagaaa atccacgccc
     1681 gtgggccggg tgagcaggat gaacaaccgg cggagattct gcatggcgat atggtgcagc
     1741 cgctgcgcgt gaaagtggat cgcgaagcac gccgcctggc gggttatcgt tacggtcgcc
     1801 agattgccga tgatttcctg acacagttag ggcagggcga agaacaggtt gcacgttggc
     1861 tggaagcgga aaacgatccg cgtctgaacg agattgtcag ccatctgaat catgttgttg
     1921 aagaggcgcg tatccgatga gtgaaaaggt ggtgttcagt caactgagcc gtaaattcat
     1981 tgatgagaac gatgccacgc ccgccgaggc gcagcaggtg gtctattaca gcctggcgat
     2041 tggtcaccac cttggggtta tcgattgcct ggaagcggca cttacctgcc cgtgggatga
     2101 atatctggca tggattgcca ctctggaggc gggcagcgaa gcccgccgaa aaatggaagg
     2161 cgtgccgaaa tatggtgaga tcgtcatcga cattaaccat gtgccgatgc tggccaacgc
     2221 attcgataaa gcccgggcag cgcaaacttc gcagcagaag gaatggagta caatgctgtt
     2281 aagtatgctg catgatattc atcaggaaaa cgccatctat ttgatggtga ggagactgcg
     2341 tgactgacgt tttactctgt gttggcaata gcatgatggg cgatgatggc gcaggcccgc
     2401 tactggcgga aaagtgcgct gccgcgccga aaggtaactg ggtagtgatt gacggcggta
     2461 gcgcaccgga aaacgacatt gtcgctatcc gcgaactgcg cccgacacgg ctgcttattg
     2521 tcgacgccac ggatatgggg ctaaaccctg gcgagatccg catcatcgac ccggctgaca
     2581 tcgccgagat gtttatgatg actacccata acatgccgct gaactatctt atcgaccagt
     2641 tgaaagaaga tattggcgaa gtgatttttc tcggcatcca gccggatatc gtcggctttt
     2701 actacccgat gacccagccg attaaagatg cggtagaaac cgtttatcaa cgactggaag
     2761 gctgggaagg gaatggcggc ttcgcgcagt tagcggcgga agaagagtag ttcgtagaca
     2821 aacatgttca cgttttggaa tttggacgtg ctattgtgac gtgtagacgg gtgtttacat
     2881 attgtgaaca cccgtgtgat caactactat agaaaaataa atcccaagga atggtagtcc
     2941 aggtttagtc attcaggcgc taataaggaa gttaaaatgc acatcatttc gaaagcgcca
     3001 tttgaagagt gtgcgcgcaa atatcctaac gacgctctgg ctctacactc attgtatcgg
     3061 gttattaagg aaacggattt ttctacgcca gaagaaatgc gaacggcttt tcccaacctc
     3121 gacaatttta aatatcgcaa caaatggtac gttctggatg tcggcgggaa caagcttagg
     3181 gttattgcct acataaactt tgttaataag cggttctttg tgaagcacat cacgaatcac
     3241 gcagagtatg acaaactgac acgttactac agagagaaca aagaatgact gctaatgctg
     3301 cacgcgctgt taaagcaaca agagaactgg tcaatgcagt cccctttctg ggaggcagtg
     3361 actcagaaga tgattatcgt gaagcactgg aactggtcga atatttgatt gaagaagatg
     3421 ataccaatcc acttatcgac tttctggcga gccgcattgc agaatatgaa aacaataacg
     3481 aaaagtttgc agagttcgac aaagccgttg cagcaatgcc ggttggcgtg gcgttacttc
     3541 gtacgctgat tgatcaacat aatctgacct atgcggattt gaagaacgaa atcggctcta
     3601 aatccctggt cagtcagatt ttatctggac aacgttccct gacgatatcc cacattaagg
     3661 cactttcagc ccgattt
//
LOCUS       DAWDRC010000402         3676 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10163_length_3676_cov_2.169290, whole genome shotgun sequence.
ACCESSION   DAWDRC010000402 DAWDRC010000000
VERSION     DAWDRC010000402.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10163_length_3676_cov_2.169290"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          853 a          986 c          932 g          905 t
ORIGIN      
        1 caaagcaatt gaagcagata tttcagctca ggcatcgcaa ggggcaacgc ctgtgctgct
       61 ggcggttgac gggaaagcag tagccctgct ggctgtacgc gatccgttgc gtagtgatag
      121 cgtggcggcg ctgcaacgcc tgcataaagc gggatatcgc ctggtgatgc taaccgggga
      181 taacccgacc accgccaatg cgatcgctaa agaagcaggg attgatgagg tgatagccgg
      241 cgtgctgcca gacggtaaag ccgaagcgat caaacgtctg caaagtgaag gacgtcaggt
      301 ggcaatggtg ggcgacggca ttaacgatgc gccagcgctg gctcaggcgg atgtcggtat
      361 tgcgatgggt ggcggcagtg atgttgccat tgaaaccgcg gcgattaccc tgatgcgcca
      421 tagcctgatg ggtgttgcgg atgcgctcgc aatttcccgc gcaacgctgc gcaacatgaa
      481 gcagaacctg ctcggtgcgt ttatctacaa cagcattggt attccggtcg ccgccggtat
      541 tttgtggccg ttcactggaa cactgcttaa cccggtggtt gccggagcgg caatggcgct
      601 ctcgtcgatc accgtagtga gtaacgccaa ccgattgctg cggtttaaac cgaaggaata
      661 agtacatcat tgcggatgcg ctgctttagg gtggcgcatc cgttataggg aaattattct
      721 acgcgacctt ttttgcacgc atacaaacac atcaaacaag tccccaactc catttgcgat
      781 ttgcgccatc cctccccgcg cgctagcatg atatttcaca aagggaggtc gtatggatct
      841 gttgtaccgg gtaaaaacgc tttgggccgc gctgcgcggt aatcattact cctggcctgc
      901 tatcgatatc tctcttcccg gcaatcgcca ttttcatctg attggcagta ttcatatggg
      961 tagccacgat atggctcccc tgcccacccg tttgctcaaa aagctcaaaa acgccgatgc
     1021 gctgatcgtc gaggcggatg tttccaccag cgatacgcct tttgccaatt tgcctgcctg
     1081 cgaggccctg gaagagcgca ttagcgagga ccaactccaa aacctgcagc acattagcca
     1141 ggagatgggc atttccccgt cgctcttttc tactcaaccg ttgtggcaaa tcgcgatggt
     1201 tcttcaggcg acgcaggcgc aaaaactggg gctgcgggca gaatatggta ttgattacca
     1261 gctattgcag gcggcgaagc aacaacataa acccgtgatt gaactggagg gcgcagaaaa
     1321 gcaaattgcc atgctgctcc agctccctga caaaggactg gcgctgctgg acgacacgct
     1381 gacccactgg cacaccaacg cacggttgct gcaacaaatg atgagctggt ggctgaatgc
     1441 accgccgcaa aataatgaaa taacgttgcc caatacgttc agtcagtcgc tgtacgatgt
     1501 gctgatgcat cagcgaaatc tcgcctggcg ggataaatta cgcgccatgc cgtcggggcg
     1561 ctatgtggtc gcggtcggtg cactacacct gtatggcgaa gggaatttgc cacaaatgtt
     1621 gcgctaaaaa aatggccaat atcgctattg gcccgtcaaa gaggaatttc atttttttat
     1681 tattatgccg tcactttaag cgacggtgta actcgattgt cgctcaatct caccatcctg
     1741 ccaatactct taggcaagat gactcttttt tttggaccgc catcaggcgt atgcttgctt
     1801 cgtttttttg tttaaggcag gaaaattacc atgacacccg cagttaaatt actcgaaaaa
     1861 aacaagattt cgtttcaaat ccacacctac gagcacgatc cggctgaaac caattttggc
     1921 gatgaagtcg tcaaaaaatt aggtttgaat ccggatcagg tctacaaaac gctgctggtc
     1981 gcagtgaacg gtgatatgaa acaccttgcc gtggccgtta cgccggtcgc aggtcaactg
     2041 gatcttaaaa aagtagcaaa agcgctgggt gccaagaaag ttgagatggc cgatccgatg
     2101 gtcgcgcagc gttcgacggg gtatctggta ggggggatta gcccactggg gcagaaaaaa
     2161 cgtctgccaa cgattatcga cgcccccgca caagaatttg ccactattta tgtttccggc
     2221 ggcaagcgcg gactggatat cgaactggcg gcaagcgacc tggcaaagat actcgatgcc
     2281 aaatttgctg atatcgcccg tcgcgattaa gcattgtgcc ggatgcgaac atccggcact
     2341 ttcggattac tgccagctca cctcaccttt cggttcataa acactcacat ccagcggcga
     2401 gcttttctgg atatacgctt tcagcacttc ggcatcaata aagccggtat tcacatagcc
     2461 cgatttgtta tcaaggcgcg gatagccatc cccgccggtg gcattgaagt ttaatgtcgc
     2521 catgcggtaa gttttcgccg gatcgaccgg ttcgcctttg attttcaggt cgttcagttt
     2581 accgtctttc gccacaaagc taacgttggc aaattgcggg taggcacctg aatctggctt
     2641 catctgcgcg acggcggtca agtaatcaat cacctcttta ccggtcatgt cggcatacac
     2701 caccacattg ccgaatggct gcactttcag tacgttttta tagctgatat cacctgcttc
     2761 gatagaatca cgaattccgc ctccgctcat caccgcaaag tcggcaccag tgcgatccat
     2821 ttgtgctgcc agaatcaacc gccccatatt ggtctgtaca aaacgcactt tgtcacgatc
     2881 gccttccaga cgaccattgg tttcgcctat tttcacttcc agctgcgctt tgcctttgtt
     2941 ctggaacggt gataacagcg agatcatttg ctggttttca gcgatttcag gggtgtacag
     3001 cacgcgctcg cttttcccgt cttcccaggt cactttcttc ttcaggttca ccggaatcag
     3061 ctggtagtta accattttca tttcgccatt acgaaactca aaatcagccc gtcccacgta
     3121 tttgccccac tcatgcgcct gcacaatcca gatgccgttt tgttgatccg gtttgcatgg
     3181 cgtacccggc acgtaatcga cctgtttttt gttttctgcc gccatacaga ccggatcttg
     3241 cgagtgacca ccgacgatca tcgccagcga tccggcaggc agcgcgcgtg ccatctccac
     3301 atcgcccggt gcgttagagc cgtgctcacc attgtcgtaa tgccccatat gggtcgccgc
     3361 gataataatg tctggctttt ctgtctgttg cagctcctga atcaccagct tcgcttcatc
     3421 agcgggctta cgaaattcga tatcagtgaa atattccgga ttaccaattt tggctgtgtc
     3481 atccgtcgtc aggccaataa cggcaatttt cagatcctga cgcttaaaca gcgcccatgg
     3541 tttaaacagg cgctcgccgg tacttttctg gtagatattg gcggaaagca acgggaactt
     3601 agcccacttt tcctgctggc gtaatacggt gagcgggtta tcaaattcat gattaccgat
     3661 cgccatcgcg tcatag
//
LOCUS       DAWDRC010000403         3670 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10183_length_3670_cov_2.230705, whole genome shotgun sequence.
ACCESSION   DAWDRC010000403 DAWDRC010000000
VERSION     DAWDRC010000403.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10183_length_3670_cov_2.230705"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1255..1264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          783 a          895 c         1055 g          927 t
ORIGIN      
        1 tgcatacctt cggcgtgacg gaagggttgc tgtcagacag tcccttttat ggtctggtac
       61 tgatttgcat tctggtggcg atctcctcac gcggcgagaa attgttattc aaaatttcga
      121 ccggcatggt gctgaccaag ctgctggtgg tcgcggcgct gggcgtgtcg atggtgggaa
      181 tgtggcatct gtataacgtc ggttcgctac cgccgctggg gctgctggtg aaaaacgcca
      241 ttattacgct gccgtttacc ctgacgtcga ttctgtttat ccagacgtta agcccgatgg
      301 tgatctctta tcgttcgcgg gaaaaatcaa ttgaagtggc gcggcataaa gcattgcggg
      361 caatgaatat cgcgtttggc attttgtttg tcaccgtctt tttctacgcc gtgtcattca
      421 cgctggcgat gggacatgac gaagcggtaa aagcctacga gcagaatatt tccgcgctgg
      481 cgattgccgc acagtttatt agcggtgacg gcgcagcgtg ggtgaaagtg gtcagcgtta
      541 ttctcaatat ctttgctgta atgactgcat tctttggcgt ctatttaggc tttcgcgaag
      601 caacgcaagg gatcgtaatg aacatcctgc gtcgcaagat acctgccgag aagattaacg
      661 aaaatctcgt tcagcgcggc atcatgattt tcgccatttt gctggcctgg agcgccatcg
      721 tactgaacgc cccggtgttg agcttcacct ctatctgtag cccgattttc ggcatggtag
      781 ggtgcctgat cccggcgtgg ctggtttaca aagtaccggc attgcacaaa tacaaaggga
      841 tgtctttgta cctgattatc gtcactggtt tgttgctttg tgtttctccg ttcctggcat
      901 tttcttgatt tacctgaaat tttaaggttt ttaatatgtt tgattcgact ttaaatccgt
      961 tatggcagcg ttacatcctc gccgttcagg aggaagtaaa accggcgctg gggtgtactg
     1021 aaccgatttc actggcgctg gcggcggcgg ttgctgcggc tgaactggaa ggtccggttg
     1081 aacgtgtaga agcctgggtt tcgccaaatc tgatgaagaa cggtctgggt gtcaccgttc
     1141 ctggtacagg aatggtgggg ctgccaattg cggcggcgct gggagcgtta ggtggaaatg
     1201 ctaacgccgg gctggaagtg ctgaaagacg caactgcgca ggcaatttcc gatgnnnnnn
     1261 nnnntgccaa agcactgctg gcggcgggga aagtctcagt taagatccag gaaccttgcg
     1321 atgaaatcct cttctcacgc gccaaagtct ggaacggtga gaagtgggcg tgtgtcacca
     1381 tcgtcggcgg gcataccaac attgtgcata ttgagacgca caatggtgtg gtgtttaccc
     1441 agcaggcgtg tgtgacagag ggcgagcaag agtcgccgct gacggtgctt tccaggacga
     1501 cgctggctga gatcctgaag ttcgtcaatg aagtcccgtt tgcggcgatc cgctttattc
     1561 tcgattctgc gaagttaaat tgtgcgttat cgcaggaagg tttgagcggt aactgggggc
     1621 tgcatattgg tgcgacgctg gaaaaacagt gcgcgcgcgg tttgctggcg aaagatctct
     1681 cttcatccat tgtgattcgt accagcgcgg catccgatgc gcgtatgggc ggcgctacgc
     1741 ttccggcaat gagtaactcc ggctcgggta accaggggat caccgcaaca atgcccgtgg
     1801 tggtggtagc agaacacttc ggagcggatg atgaacggct ggcgcgtgcg ctgatgcttt
     1861 cgcatttgag tgcgatttac atccataacc agttaccgcg tttgtctgcg ctgtgtgccg
     1921 caacgaccgc agcaatgggg gccgctgccg ggatggcatg gctggtggat gggcgttatg
     1981 aaaccatctc gatggcgatc agcagtatga tcggcgatgt cagcggcatg atttgcgatg
     2041 gtgcatcgaa cagctgcgcg atgaaggttt cgaccagtgc ttcggctgcg tggaaagcgg
     2101 tgttaatggc gctggatgat accgccgtga ccggcaatga agggattgtg gcgcatgatg
     2161 ttgagcagtc gattgccaac ctgtgtgcgt tagcaagcca ttcgatgcag caaacggatc
     2221 ggcagattat cgagattatg gcgagcaagg ccagataaac gccaggtttt gggtattgtg
     2281 ccggataagg cgtttacgcc gcatccggca acttgtcttt acgcggtagc ttcatcttcg
     2341 gctaaacgcg tttcagcatc cgcgacgatg gcttccagct cgctcaacat ctcttcatag
     2401 ccaaaatttt tggcggtacg agcaggttct ttcgccacca cgggcttcac tggctggcgc
     2461 tttttggccg attttttact catcatcatc tccctgtaaa aacaactaga caggcaaatc
     2521 tatcagcaaa gcgcgcagtg gggaatcggc aaccagcgta atgttagcct cgtcacgaat
     2581 aaacgcccca tcaccgcagg ttagtgccgc tttctcttca tgatgcgtaa gcgcatgaaa
     2641 tttcccgtga atcgattgca aatacgcgcg tggcccatgc aactggaaat tcgcactttc
     2701 gcctttgtcg accacgatat ggttcagcca ctcttgccgg cgcaattgca ggcttcccat
     2761 cccaccttct ggcgaggcga ttaattgctg cttgctcata ttaagcgcca gcttttgaat
     2821 cagcggattc tctcgctgcg ggcaggcatc cagccaaagc tgcattcgcg ttaacggttt
     2881 gtctttgctg agattgtgtt cgctatagct gacacccggc tgggtagaga gcagcaacac
     2941 ctcaccggcg ctggcctgaa catgattgcc ttcgctgtcg cgatactcag cttctccatc
     3001 cagaatcaca tttaaaatat cgactttggg ataggttcgc ggctgaaagg cggcacctgg
     3061 ggccagcact tcctggttaa gcacgcgcag ggaggcatag cctaacaatt tcgggtcgaa
     3121 gtagtgtcca aaggaaaaag tataccgggc ctgcaaccat ccgtagtctg cttgtccaca
     3181 ctgcctggca gttcgggtag taatcataat tcttgacctc tctttacttc cttttatggt
     3241 aaggggctgg acgctacatt gttagccaga tattctgccc ggtatgttca aatttcctga
     3301 atgagaacga aatggccaaa gaaagggcat taacgctgga agcactacgg gttatggatg
     3361 cgatcgatcg ccggggcagt tttgcggcgg cggcggatga gctgggacgc gtgccttccg
     3421 cacttagcta caccatgcaa aaactggaag aagagctgga tgtggtgctg tttgaccgct
     3481 cgggccatcg caccaaattc accaatgtcg ggaggatgtt gctggaacga gggcgcgttt
     3541 tgctggaagc cgcagataaa ctgactaccg atgcggaagc cctcgcgcgt ggttgggaaa
     3601 cgcatctcac cattgtgacc gaggcgctgg taccgacacc tgcctttttc ccgttaatcg
     3661 acaaactggc
//
LOCUS       DAWDRC010000404         3668 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10189_length_3668_cov_2.516468, whole genome shotgun sequence.
ACCESSION   DAWDRC010000404 DAWDRC010000000
VERSION     DAWDRC010000404.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10189_length_3668_cov_2.516468"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          966 a          927 c          948 g          827 t
ORIGIN      
        1 ggaccagcac gggaggccgc gttgctcaaa ggcggcgcgt aatgcaggca tctcgttgtg
       61 caactcttcc ggcattggcg gtcgacccgc tttgtaatct ggtaaacgct gatgacgcca
      121 gccgctactg cggttttcat catcaaaaac ggcgaccgcg tgggttggct ggctgtgcat
      181 aatgagctga tcgagcgcgt gctggcaggt ttcgacacag ggtgaaccct gaacggcatg
      241 aatgcgacga ataagattca gtgcatcgac aataagcaaa tgaacagcca cgataacccc
      301 cttaaagatc aggcgataag ggtaacattc gatgccagaa caggctatgg ctggatgaga
      361 aatcgggaag aggcctcgca aaaagaggcc tctggagagc gattaatcgc aggcaacgat
      421 cttcattgcc aggccgccgc gagaggtttc gcggtacttg gcgttcatat ctttacctgt
      481 ttcgtacata gtttcgataa ctttatcgag gcagacgcgc ggctcgctgg tacggcgcag
      541 cgccatacgt gcagcattca ccgcttttac tgctgcaatg gcgttacgct cgatgcacgg
      601 tacctggacc tgtccggcaa ccgggtcaca cgtcagaccg aggttgtgct ccatggcgat
      661 ttccgccgcg atgcatacct gcgccgggct tgcgcctaac agttctgcca gaccagctgc
      721 cgccattgag cacgccacgc cgacttcacc ctggcagccg acttcggcac cagaaatcga
      781 ggcgttcatc ttataaagag aaccaatggc gctggctacc agcaggtaac gagccagtga
      841 gttagcgttc acttcgcgga taaacttgtc gtagtacgcc agcacggccg ggataatccc
      901 gcacgcaccg ttagtcggcg cagtcaccac acgaccgcca gcagcattct cttcgttcac
      961 tgccagtgca aacatgttga tccagtcaac aaccgccatc gggtcagtgg tggttttatc
     1021 ctggctgacc agcatccggc gtagtgccgc agcacggcgt ggaacgcgca gtttgccagg
     1081 caacacgcct tcggtagaaa taccgcgctc aataccgcca cgcatgactt cccagacgtt
     1141 cgccaggtgc tgttccagct cttctttgct gtgcagcgcc agctcgtttt tcatcatcag
     1201 gccagagagt gacagcccgg tttcctgaca atgtttttgc agatcggctg ctgaactgta
     1261 cggataagga acttcaaccg gtgcgctatc ctgctgacca aaatgttctt catcaacaat
     1321 aaagccaccg ccgatagagt aataagtctg gctgtaaacg actttatcgc ccgccagcgc
     1381 ggtaatgcgc ataccgtttt catgcagaga aaggttgtcg gcatggaagt tcatgcactg
     1441 atcaaccggg aactccactt catgctgacc gtttgccagc atcaggcgac catgagtatt
     1501 cacatcctga ataaaaccag ggatggaatc gatatccacg gtatccggca ggttacccgc
     1561 cagacccata attatggcga tatctgtgtg gtggccttta ccggtcagag agagcgagcc
     1621 gtacacgtca accaccacgc gggtcacgtc tttaagcagg ttacgggcaa tcagatcgtc
     1681 ggtaaattgt ttacccgctt tcattggtcc aacggtatga gaactggaag ggccaatgcc
     1741 gattttgaaa atatcgaata cgctaatcat aggaaataca tcgcgttaaa acggaggaag
     1801 cgccgcccga aagcggcgcg aaaggactta gctgaacaga gagtagaaga ttgcggagat
     1861 tgcaatcaga cccatcacga caacgaatac gttgctgatg tgaccgctgt acttacgcat
     1921 tgccggtact ttctgaattg cgtacatcgg catcaggaac aggatcatcg cgatgattgg
     1981 gccgcccagg gtttcaatca tacccaggat gctcgggttc agggtggcaa caatccaggt
     2041 cgttaccagc atgaacagcg cagtgatacg gttcagcttg ttgatttcga tagacttacc
     2101 tttaccacgc agagatttaa tcaccatacc gttgaagcct tcacgtgcgc ccaggtagtg
     2161 gccgaggaag gatttggtga tagcgataat cgcgataatc ggagccatcc acgcgatgat
     2221 cggcgcgtta aagtggttag ccaggtaaga cagaatcgag atgttctgct ctttagccgc
     2281 agccaggtct gccggagtca ggctcagtac gcagctgaag acgaagaaca ttacggtcag
     2341 caccatcatg atgtgtgcga atgccaggat cttcgagcat ttctgttctg ccatatcgcc
     2401 gtactcttca cgcttcgcaa cggcgaaaga agagatgatc ggagagtggt tgaacgagaa
     2461 caccattacc ggaattgcca gccacagtgt catccacaga ccgtttccgg ttgcagatgc
     2521 agtgtccaga gacagcgttt ccagtgctgc gccgttccac tgcgggatca ggtacagagc
     2581 cagcagcatc agtacgccaa caaacgggaa taccagaata ctcatcgctt taacgatcat
     2641 ctgctcaccg aagcgaacga tggtcatcat acccacgatc aggatcagcg acagaatcgc
     2701 acgcggcggt ggcgtcatac ccagctggtg agacatgaag ctttcaactg tattggtgat
     2761 tgccacgcta taaaccagca ggatcgggta gatagcgaag aagtagagca gagtaatcag
     2821 tttacctgcg ccaataccaa agtgttcttc tacaacctcg gtgatgtctt cgcccgggtt
     2881 tttaccagac agtacgaagc gagtcaggcc gcggtgagca aaaaacgtca tcgggaacgc
     2941 aaggatagcc atgatgatca gcgggatcat accgccaaca ccagcgttga ttggcaggaa
     3001 cagcacgccc gcgccgattg ccgtgccgta aaggcccagc atccacatgg tgtctgtctt
     3061 gcgccaggca ctacgagagt ctttcgacgc aatcgtgctg gtttgagtcg tttccatcta
     3121 tttctcctgg aggaatgcaa ataattcagc ttttagccag ttcagcccgg gaaggtgcct
     3181 gaccgtaaaa atgaaatctg tctatcggcg atggcttaat cattttccac cgtaataatt
     3241 tgagggcgga aagatacaca caagttatgt atctatcagt gatcttgatc ccaaatacaa
     3301 taatctactt ggaagataga taatcacaac gctttactct gtaaatcaaa agtaaaagag
     3361 aatttaaggg caatatttac ccattaaacg acatctgact tcttacgggg cgcgcaatat
     3421 atgtaggttg aactgaaaaa atcaatactt ttagtcaatc atggcgataa tttctttttc
     3481 atggcaataa aatatggttt atgaaaatag ggaaacgttt gcgtttcaac ataaattgta
     3541 tgacatatta actgtggtga tatttgtcgc gtgagggtgg taagaaacat tcatataaca
     3601 aatgaaatga gttgatgagt aagtgaccaa aaatgagaaa acccggcaac aaacgtcacc
     3661 gggggagg
//
LOCUS       DAWDRC010000405         3665 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10203_length_3665_cov_2.126316, whole genome shotgun sequence.
ACCESSION   DAWDRC010000405 DAWDRC010000000
VERSION     DAWDRC010000405.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10203_length_3665_cov_2.126316"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3404..3413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          834 a         1006 c          926 g          889 t
ORIGIN      
        1 cggagcaaat ctcctgggat cactggaaac tggcgttagg ttttagcgtg gaacaggctg
       61 atggtcgcat tacgccaccg ccattcccgg tactgctgtg gctgtggaac tcggtaaagg
      121 tcgccgggat ttccgcgatt ggcattgtgg cgctctccac cacctgcgcc tacgctttcg
      181 cccgtatgcg ctttccaggc aaagcgacgc tgctgaaagg aatgctgatt ttccagatgt
      241 tcccggcagt actttcactg gtcgcgttgt atgcgttgtt tgatcgtctg ggtgagtaca
      301 ttccattcat tggcctgaat actcacggcg gcgtaatttt cgcgtatctg ggcgggattg
      361 cgctgcatgt ctggaccatc aaaggttatt tcgaaaccat cgacagttcg ctggaagaag
      421 cggcggcgct ggatggtgcg acaccgtggc aggccttccg ccttgtcctg ttgccgctgt
      481 cagtaccgat tctggcggtt gtgttcatcc tgtcgtttat cgctgccatt actgaagttc
      541 cggtcgcgtc gctattactg cgtgacgtaa acagctacac cctggccgtg gggatgcagc
      601 aatacctcaa cccgcaaaac tacctgtggg gtgactttgc cgccgctgcc gtgatgtctg
      661 cattaccgat caccatcgtc ttcttgctgg ctcaacgctg gctggtcaac ggcctgacgg
      721 caggtggtgt gaaaggttaa agatgttgtt ctgccaatgt tatgccgctg caccctcaac
      781 ttacgttatc ccaacttgtg actgttattc ggcgctccac ggagcgcctt tttttactcc
      841 cgcttcagcc gtttcgaatt acacaaccac agggtgatca ccagtaacag gatcgctgcc
      901 gagtagatca acacatccag tggcgattta tgatcgacga tgatcaagcg cacaatggcg
      961 gtgatcccaa tatagacaaa gtaacgtagc gggaagtgaa aaccggactg aaagtacttc
     1021 acaatcagcg cgataaattc gaaatagaga aagtaaacca ccagaccttc taccagctca
     1081 tatttactgg tttgttctgg cgcgaacagc acatcagcca gatgcaccgt ttctttgccg
     1141 aggaagacaa ccaaaatcag gccgaggcac aacaggccaa gattgagtac ggtctgcaaa
     1201 atggtggaga taaactccac gcgcggacga gagagtgacg tcatagcagt acctccttca
     1261 cagtggcgag cttcctgtat aacgcaaaaa tgtgacggag atctatattt tttgtgtatg
     1321 ttttgttcag ctcaattaag tgggcatctc ccatcaacgt cgcatctggc aaaacacaca
     1381 gcccaaaaaa atcacccatt caggtgattc ccagccacca gcaatgcgcg aaaaatcgac
     1441 aatgttcctc tggaacaaat gaaacgtaac ctgataaagg tggtaaaaat gaagccttat
     1501 tttgctgctt tgatgttatc agtctctgtt cttcctgctt atgctggtcc gctcggtact
     1561 gctgataaag ccgacctacc gcaaagcaat gtctccagtc caatgatggc ccagtcgctc
     1621 aggcaaccag acctgcaacc gatatcgacg gacaggaaga cagaatgttt tcgcctttac
     1681 acaccagatc gcaaaccggg ggtgaactgc gttcctgatg gctcgacagg ccattaagaa
     1741 tgaaggtgcc agtgagtgcg aaaactggca cctttcccat gacttagcgg aaattgacgc
     1801 tacggtcgct ggtcatatca tacaactgga acttacgccc aagttgctga ccaccgtcac
     1861 gcgtcagcgg cgtccagcca atcgccgcac ggctgcgtgt cgggccagac gagaagagat
     1921 ccagcggtac agagacatac acgcctttgg tgaagtcccc ttcgccgtac tcctctttcg
     1981 aaacattcgt gatcgtggca tagcccccca ctaccacgcc gctatcaaag cgtttggcga
     2041 tctccagcgt gccaccttta tccccagcca gatactgccc gacgctcgct ttcactaaca
     2101 cgtcctgggc gaaagatggc gtccagtacg cggtcagatg tccggttttc acgctgtaat
     2161 cggtgaattt catcatatct tttgcgctac gccagtcgcg ctgtttaacg tagttggcat
     2221 ccagaccaaa cgcccagttg ctatccagcg ggcgatacaa cacttctgcc cccacgccgc
     2281 caaacatggt ttcgagataa cctccgtaga cctgaccgta gaagccgttg cccagatgct
     2341 ggaagtagtt ggcttgcagg ttattcacat agacatcatt ctgcacatac tcgcgcacat
     2401 gggtacgcac gcgcggtaag tgcgagtcct gcggtggatt ggtgtagtta aacttgtcgt
     2461 agttgttggc aaggttagca aacaggctgc cggtggtcag cagatggtcc gtcagccaca
     2521 aatccgccgt tcccatcacg cccagctgat acatgtaaaa gttttccggg ccaccgaccg
     2581 actggttcag caccggatcg atatggaaat caaagcgtga tttgtcgata taccagccct
     2641 gctcggtgga cttcggaacc actggctcga cgcgtttttg cgccagcgtc gtttcgtggc
     2701 ccaacggctc tccgccgaga tgatttttca ggctggcgac atcggtttcc gtcgtcacct
     2761 gtggcatatt aaggcgattt tccgtaatgc ggatcgtttt gatcccatcc ggcagatcgt
     2821 tcatcacgat ccgattagca cggatgatcc cttcgcgcga atcacgatat ttcacctgct
     2881 cgccggtaac atacagcgta tcgcctttcg cctggatctg cggatcggcc agtccggcgt
     2941 tgtatttcaa cagcgttaac tgattcgcca ccaccgagtg ctgcaaaatg gcatcctgcg
     3001 gctgcggttg atattgcggg cgggcgttat cgttgtaaga cgggcgcaga tcgttaaagt
     3061 tggtgcgcag cgtaacgcca aacataaagg tgttgccacg ttcatagcta aggttaacgt
     3121 cggcccaatc ggtaacgcga taaatcgccc cgacgttaaa cttacttttt tgctccagct
     3181 tcccggcaaa atcctgctga taattattgc cttcatactc cagtttcagg cgcagcggtt
     3241 gccagggcgt ctggtattcc acgccgccaa acagtgaggc cgggccgtgg aacatctggc
     3301 tgccgtcgat agatcccgcc tgtttgtagc tgttatcgcg atagcaatat ttatcactgg
     3361 ctgaacagag cggatttttc acattaccgc tggtgcccag atannnnnnn nnnatattcc
     3421 gcatcaaaca gccccgtacc gccgatatcc cgcgcaccaa ccgccacttg cggcagccag
     3481 taactctctt cccacaaacg cagtttgaga tcgaaggctt tatctttata cgtttgatcg
     3541 ccagagaacg cttcgacgct gctgtactgc cgggtgcgca cgtcggtgta gcgcagcgtt
     3601 gtttccagcc acgggaagag ttgcactgaa gctgagtaat aacggtactg atcgttgtcg
     3661 cgcta
//
LOCUS       DAWDRC010000406         3659 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10221_length_3659_cov_2.135960, whole genome shotgun sequence.
ACCESSION   DAWDRC010000406 DAWDRC010000000
VERSION     DAWDRC010000406.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10221_length_3659_cov_2.135960"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1240..1249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3108..3117
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          807 a          936 c          949 g          947 t
ORIGIN      
        1 gccctggact tcaattttca tggtcttcgc acgaccctgt ttcagggttt gcgttgccag
       61 tacgtgttca acttcggaag tgccgatacc aaaggctagt gcgccaaacg cgccgtgggt
      121 ggcggtatgc gagtcgccgc agacaatggt catccccggc aaagtgacgc cctgttccgg
      181 ccccattaca tggacgatcc cctgatacgg gtgattcagg tcatacagct cgacgccaaa
      241 ctctttgcag tttttgatca gctcctgcat ctggatgcgc gccatttcac cgcaggcatt
      301 aatgtctttg gtctgagtag agacgttgtg atccatagtg gcgaaggttt tgcctggctg
      361 acgtaccggg cgaccgtggg cgcgcagacc atcgaacgcc tgcggtgagg tcacttcatg
      421 caccaggtgg cggtcgatat ataacagcgg ggtttcgttt tcggcttcgt acactacgtg
      481 agcgtcgaat aatttttcgt ataacgtctt agccatgatt acaccccttc tgccacatag
      541 cgggcaatga tatcgcccat ttcatcggta ctaacggcgg cagcgccacg ggctaaatcc
      601 ccggtgcgaa tgccttcttc taatgcgcgg ttaatggcgc gttcaatggc ggaagccgca
      661 tcatcggcat ccaggctgta acgcagcagc agcgccagcg acagaatttg tgcaatcggg
      721 ttggcgatat ttttacctgc gatatcgggt gccgagccgc ccgccggttc atacagacca
      781 aaaccttgct cgttcaggct ggcggaaggc aacatcccca ttgagccagt gatcattgcg
      841 cactcatcag acagaatgtc gccaaacagg ttggagcaca gcaagacgtc aaactgtgat
      901 ggatctttaa tcagctgcat ggtggcgttg tcgatgtaca tatgcgccag ttcgacgtcc
      961 gggtattccg tggcgatctc gttaacgatc tcccgccata aaatagagga ttgcagcacg
     1021 ttggctttgt cgattgaggt gactttgtgg cgacgcttgc gggcagattc aaatgcgatg
     1081 cgggcaatac gctcgatctc aaaacggtgg tacacctcgg tatcaaacgc tttttcatat
     1141 tgtccgctac cttcgcggcc ttttggctga ccgaaataga tgccgccggt cagttcgcgc
     1201 acacacagga tgtcaaagcc gtttgcggca atgtcagcan nnnnnnnnna agtgcttacg
     1261 caaaggcagc agtgcgccgc gttctggttg ctggtcttgc ggcaaatgtt cccacttcgg
     1321 accacctacc gagccaaaca gcacggcatc ggcttgctca caaccttcaa ccgtcgcagg
     1381 cggcagcggc tgcccgtgat tatcaatggc tgcgccgcct acatcgtaat ggctggtggt
     1441 gatacgcatc gcaaatcggt tgcgcacggc atccagcact ttcagcgcct gggtcatcac
     1501 ttccggacca ataccgtccc ccggcaatac ggcaatatgg taattcttcg acatcacacg
     1561 gtttccttgt tgttttcgtt gtgttgagct ttgcgttgca actctttttc gacttctgcg
     1621 gcacgccaga tattgttcag tacgtgcacc atggctttgg cggaggactc gacaatatcg
     1681 gtggccaggc cgacgccgtg gaagcggcga ccgttgtagt tggcgacaat atccacctga
     1741 cccagcgcat ctttaccgtg gcctttggcg gtcaggctgt atttcaccag ttcgacgtta
     1801 tagtcagtga tgcggtttat cgcttggtag acggcatcga ccggaccgtt accgttggcg
     1861 gcttctgctt tgacttcttc gccacaggcc agtttgacgg cggcagtggc aatatcgtta
     1921 gaacctgact gcacgctgaa gtaatccaga cggaaatgct ccggctcttc ttgctgctta
     1981 ccgatgaagg ccagcgcctc cagatcgtaa tcaaacacct ggcctttttt gtccgccagc
     2041 ttcaggaaag cgtcgtacag gttgtctaaa ttatattcac tttctttata tcccatctca
     2101 tccatgcgat gtttcaccgc cgcacgcccc gaacgagagg tcagattcag ctggatttgg
     2161 ttcagaccaa tagattctgg tgtcatgatt tcgtagtttt cgcggttttt cagtacacca
     2221 tcctggtgga taccggagga gtgtgcgaat gcgccgctgc caacaatggc tttgtttgcc
     2281 gggatcggca tattacaaat ctggctaact aactggctgg tgcgccatat ctcctggtga
     2341 ttgatggcgg tgtggacgtt gagaatatcc ttacgaactt tgatcgccat gatgacttct
     2401 tcaagggaac agttaccggc acgctcgccg atcccgttca ttgcgccttc tacctgccgt
     2461 gctccggcat gtaccgccgc cagtgagttg ccgaccgcca ggcccaaatc gtcgtgggta
     2521 tgtacggaaa taatggcttt gtcgatgttg ggtacgcgtt catacaggcc gctgatgatt
     2581 ccggcgaact caaatggcat ggtgtagccc acggtgtccg gaatgttgat ggtggtggca
     2641 ccggcattaa tcgccgcttc gaccactcgc gccagatcgg caattggggt gcgtccggca
     2701 tcttcgcaag aaaattcaac atcatcggtg taattacggg cgcgtttcac catatagata
     2761 gcgcgttcga tcacttcgtc cagcgtgctg cgcagcttgg tggcgatgtg cattggcgaa
     2821 gtggcaataa aagtatggat acggaaggct tcggcgactt tcagggattc ggctgccacg
     2881 tcaatatctt tttccacgca gcgagctaac gcacatacgc ggctgttttt gacctggcgg
     2941 gcgatggtct gcaccgattc gaaatcgccc ggcgaagaga cggggaaacc gacttccatc
     3001 acgtcaacac ccatacgctc aagggccagc gcaatttgca gtttttcttt cacactcaag
     3061 cttgcctgta acgcctgttc accgtcgcgc aatgtggtat cgaaaatnnn nnnnnnnctt
     3121 gtctcttttt gagcgcatcg cttcgggcat aaaaaaaccc gcgcaatggc gcgggttttt
     3181 gtttgactgc gtgctggctt aatgctggat gccgctcact cgtctaccgc gcaaagaaga
     3241 tgcgtttagt agtagtagac cgataaagcg aacgatgtga gtcattaaat cagctccaga
     3301 tgaatgcgat atgcttttag agttactgga tacaaaaacg gatgtcaacc ctgaagcaat
     3361 aaaaacgtca cgccattgtg agttcggcat ccgtaaaatt agctaattgt gctgcggtgg
     3421 ttaaaacaag cgatattaat ttctgcttaa ccgccgatgc ttttcatcgg ttgacatatt
     3481 tcagaataat tttttgcatc taatcaacga gggaaagggg gataaaatgc acgcgttaca
     3541 aaacctatcc tgatgatttg tattgaatta tatgttttgc gaattttttg atattcattt
     3601 ggtgaatatt attgatcaat taatgttaag aattaatgca ttaaacatat aaattaatt
//
LOCUS       DAWDRC010000407         3656 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10232_length_3656_cov_1.875868, whole genome shotgun sequence.
ACCESSION   DAWDRC010000407 DAWDRC010000000
VERSION     DAWDRC010000407.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10232_length_3656_cov_1.875868"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3289..3317
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          832 a         1088 c          955 g          752 t
ORIGIN      
        1 ccggcactga tcttaatgga cgaactggcg cattactgtg cgccagttcg tccattaaga
       61 tcagtgccgg acggcgggcg agggcggcat cgagatcaaa ctcgctgatg tgccgcccac
      121 ggtacgcctg gcgttttaac ggcagaacag ccagaccttt cagcattgcg gcggtatctt
      181 tccgaccgtg ggtttctacc acgccaacca caatatccag cccttgcgcc cgcagtcgct
      241 gggcttccgc cagcatcgcc caggtcttcc cgacgcctgc gcaggcaccg aagaaaactt
      301 tcagcttccc ccgatgcggc gcggcagctt gttccagcag acgatcgggg tcgggacgta
      361 agggttcgtt attcatcaag cttatccagc gccagattga gttcgacaat gttgacgacc
      421 ggctggccga tatatttcac cagcggttgt tggctgtatt ttgcgatcag ttgcgtgagc
      481 tgttcaacgc tgagattacg cgctttcgcc acgcgtggga tctgccaggc cgccgcttgc
      541 ggggtgatgt tattgtccag cccgcttgct gacgccgtca ccagttcaac cggaacgttc
      601 gtgctggcat tcgggttagc agcccgtaat gcagcaacgc gtgcggctat ctgtttatcc
      661 agctccgggt tactgaccgc caggttgctc ccgccagaag cctgtggatt atagggcatt
      721 tctgctgttg ccgacgggcg accatgaaaa tagccgttgc cggtaaaatt ttgcccgatt
      781 aatgccgaac cgcgcaccgt atcaccttca cgaatcaatg aaccgttggc ctgccaggga
      841 aaccaccatt gccccagtgc ggtggtcagc agcgggtaaa cgccgccagt aatcaataac
      901 agaaagagaa atgtcgataa tgccggacgt aatccactca tggtaaacct cacaccagac
      961 cgcaaacggt tagcagtaaa tcaatgactt tgataccaat aaacggcacc agcagcccac
     1021 ccagaccgta aatccataag ttacggcgca acatggcaga agcggtaagc ggtttataac
     1081 tcacgccttt taacgccagg ggaatcaaaa agacgataat caaggcgttg aaaatgaccg
     1141 cactgagaat ggcggagtcg ggcgaatgca ggcgcatgat gttcagcgca tttaactgcg
     1201 gatacgttgc cgcgaacgcc gctggaataa tggcgaagta tttcgccaca tcgttggcaa
     1261 tgctgaaggt ggtcaacgag ccacgggtca tcagcatctg tttgccaatg tgcactacct
     1321 cgatcaactt ggtcgggttg gagtcgagat ccaccatatt gcccgcttct ttcgccgcct
     1381 gggtgccgga gttcatcgcc accgcaacat cggcctgtgc cagcgccgga gcatcgttgg
     1441 tgccatcgcc ggtcatcgcc actaaacggc cttccgcctg atactgacga atcagtgcca
     1501 gcttggcctc cggtgttgct tcggcgagaa aatcatcgac acccgcttcc gcagctatcg
     1561 cggcggcagt caggcggtta tcgccggtaa tcatcaccgt tttaatgccc attttacgca
     1621 gctgggcgaa gcgctcttta ataccgcctt tgacgatatc tttcagcgca ataacgccca
     1681 gcacacgaga accttccacc accaccagcg gcgtggctcc ctgacgcgca acctgatcga
     1741 ctttttgatc aacatcggca gggaagtgac cgccgttagc ctcaacatgg cggcgaatgg
     1801 catcgacaga acctttacgg atcatgcggt tgtcgatgtt gatcccgctc atccggcttt
     1861 gtgcagtaaa cggtacaaag gtggcatgga gcgactgcac atcgcgctcg cgcaggttaa
     1921 aacgctgctt ggcgaggatc acaatactgc ggccttccgg cgtttcatca gccagcgaag
     1981 ccagttgtgc ggcgtcggcc agcgcttttt cctccacgcc ttgtgcgggg ataaactctg
     2041 acgcctgacg gttaccgagg gtgatggtgc cggttttatc cagcagcaga acgtcaacat
     2101 cacccgctgc ttcaactgct cgcccgctgg tggcaataac attcgcgccc agcatccggc
     2161 tcatcccggc gacgccaatc gctgacaaca ggccgccaat agtggttggg atcagacaga
     2221 ccagcagtgc caccagtacc gttacgctga cggcattacc gccccatgcg gaaaacggcc
     2281 acagcgtggc ggttgccagt aaaaagacga tagtcagggc aatcaggaga atggtcaggg
     2341 caatctcgtt cggcgtttta cgtcgctgcg cgccttccac catcgcgatc atccgatcca
     2401 gaaatgtctc gccggggtta acgctgcact caatcaccag ccagtcagaa agaatacgcg
     2461 tgccgccagt gacggaggca aaatcaccgc cggattcacg gatcaccgga gccgattccc
     2521 cggtgatggc gctttcatcg accgatgcgc cgccttcaat gacttcacca tcgcagggga
     2581 taatatcgcc agcttctacc agcacgatgt cacctttacg cagttgatca gcaggaactt
     2641 tgtccgccgc agcgccatat ttcggttcgc gcagtttgcg agcaaaagcg gtttttttca
     2701 cccctttcag actgttggcc tgcgctttac tgcggccttc tgccaacgcc tcggcgaaat
     2761 tagcgaacag tacggtgacc cacagccagc cgctaattgc cgcgctaaac agcgcattgc
     2821 caggcatcac accgcttgcc atcgcgatgc taatacaggt ggtcagcaga ctgccgatcc
     2881 agacgataaa catcaccgga ttgcgccatt gcgcctgcgg gtttaatttt ttcaccgctt
     2941 ctttcagcgc ctgaacgaca agtgttggtt cgaatagcgc cagttgttta cgactcatat
     3001 tcagtgctca ctcaatatca tcaggagaga tattccgcca ccggaccaag cgccagggca
     3061 gggataaagg tcagtgcgcc aaccagcaac acggtgccga ttaacaggcc aacaaacagc
     3121 gggccgtgcg ttggcaatgt gccggagctg gcgggttggc tctttttact caccagcgaa
     3181 ccggcaattg ccatcaccgg gataatcacc ccgaagcgcc cgacaaacat gcacaacgcc
     3241 agtaaacagt tccagaatgg agagttggcg cttaatccgg caaaggcgnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnntac agcacttcgc taaaaccatg cgggccaggg ttgagcatgg
     3361 cgctacgtcc ggcgtcggtc atcattgcca gcgccgcgcc catcagcacc agcgtcgggg
     3421 tgaccagaat cgccagtgca gtcagtttca tctcgcgtac gtcgattttt ttacccagat
     3481 attccggtgt acgaccaatc atcagcccgg caataaacac cgccagcagg acaaacagca
     3541 tcatgccgta aaggccggaa ccgacgccgc cgaataccac ttcaccaatt tgcatcaacc
     3601 acatcggcac catgccacca agagcggtaa acgaatcatg catcgcaatc accgcg
//
LOCUS       DAWDRC010000408         3649 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10248_length_3649_cov_2.420979, whole genome shotgun sequence.
ACCESSION   DAWDRC010000408 DAWDRC010000000
VERSION     DAWDRC010000408.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10248_length_3649_cov_2.420979"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2051..2060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          810 a          940 c         1029 g          860 t
ORIGIN      
        1 cgtgttcaga cggcgaatac caatttcaac accgcaggat tcgcagtagc cgaaatcttc
       61 gtcttccact tttttcagcg tcttctcgat ctttttgatc agcttacgct cgcgatcgcg
      121 gttacgcagt tcgaggctga actcttcttc ctgggctgca cggtctaccg ggtccgggaa
      181 gttggctgct tcatcctgca tatgtgtaac ggtgcgatcg acttcatccc tgagttgatt
      241 acgccatgct tccagaatac gacggaagtg cgccagctgg gcttcattca tatactcttc
      301 gcccggcttc tcctgatatg gttccacccc agcgatggcg agaatactca gggacgatgt
      361 tttacggttt tgcccttctt gcatgttgct tctccttaac acgcactatc gatccccatg
      421 ttcgggggaa aaatgaggcc gctataaata gcagatgctt ttccggatag caattatcta
      481 aacgtaacac ttgacaactg tgtgaggaaa agcgtatttg cgcacgcgac cagaatgtaa
      541 attaaccagt tacttactct actacaatgt aaccggtagt gattttttaa gagccatgcc
      601 ttcggcagaa agttccgctt tgtaagccag aatttctacc cccctctgtt gagcttctga
      661 caatagttgc gcgtatttct catcgatgtg gcgcgcgggt gaaaaccgtg taatggctga
      721 atgcagcacg gcgaaaaaga taaccgcacg ctggccttca gccgctacgc tcatcaactc
      781 ccgaaggtgt ttctgacctc gttcagtgac cgcatcggga aaatatccct gttcgttctc
      841 cgctaacgta accgatttca cttcaatata gcagtctgga cgcgaatccg cctgcaacat
      901 aaagtcaata cggctgcgtt cggcgccgta ttttacttcg cttttcagcg agctatagcc
      961 tgacagctct gaaattgatt cattaaggat agcctctttc gtcaacctgt tagcccaaag
     1021 cgtattgacg caaataattg cgccgctctg gctttgagtt aattcccagg tgtgtgggta
     1081 tttccgtttg gtgttgtctg aagtcgaata ccagacggta tcgccaggcg ttgcacaacc
     1141 gatcatcgca cccgtattcg ggcagtgtag cgttaattcg cgaccatcgg gtgtgatcac
     1201 atcggctaaa aaacgtttgt aacgctgaat tagcgtcgcg cgctgtagag ggggagaaaa
     1261 ttccattgcg acaatccttg ttattgcgtt agcgcccagc gttttagcgg cgtgtagcgt
     1321 gtgcgtccac gggcaaacga ggaggcgtaa agggtgaact ccgtcaccgt atacgaccag
     1381 ttaaaacctg gcggcgggat tgtcaccgcc tcgctggcgt cgcgcaataa ggtaatatgt
     1441 ggatgaaacg gacgattgct ttgaaaacaa ccgctgcggg cagcctgtga acggagcata
     1501 ttcgccagct ggattaagcc gcgtggtggc tgtcgcatcc ctaaccacac cacacgcgaa
     1561 cgcagccatt gaccggcgtc atcaagcgtg agtgtgaaac caggttgacg aatccgtccg
     1621 gctaaaagag aaagcgcctt ctctttctct gcgctcactt cgcctaaaaa tgccagagtc
     1681 agatgcaaat tatcggcggc gaccggacgt cccgcctcag gtgggaagtg tgtggcgcgc
     1741 cagtggataa tctgttcgcg gatttctgca ggtaagtcga tagcaaagaa cagccgttgc
     1801 ggttcagaca tgtgaggcac tcggttatga attaccgcga tgctacaatg tggcgcgaag
     1861 aatgttaacc ctctggagcg ttttgtgtcg tcgttgcccg ttgctgccgt cttacctgag
     1921 ttacttgctg ccctcgatgg tgcatcgcag gtgttattaa gtgcgccgac cggggccggg
     1981 aaatcaacct ggctgccgct gcaactgctg gcgcatcccg gcattaacgg gaaaattatc
     2041 ctgctggagc nnnnnnnnnn cgtaacgtcg cgcaacgact ggcggagctg cttaacgaaa
     2101 agccaggcga taccgttggc taccggatgc gtgcgcaaaa ctgcgtcggg ccgaataccc
     2161 gcctggaagt agttaccgaa ggcgtgctga cgcggatgat ccagcgtgac ccggaactga
     2221 gtggtgttgg attggtgatc cttgatgaat ttcatgagcg cagcttgcag gcggatttgg
     2281 cattggcgtt gttactcgat gtgcaacaag gtctgcgtga tgaccttaaa ctgctgatta
     2341 tgtcggctac gctggacaac gaccgcttgc agcaaatgct gccagaagcg cccgtcgtca
     2401 tctcagaagg gcgctcgttt ccggttgaac gccgttattt gccgctgccc gctcatcagc
     2461 gttttgacga agccgtcgcg gttgctaccg ccgaaatgct gcgtcaggaa agtggatcat
     2521 tactgttatt tttacctggc gtcggagaaa ttcagcgtgt gcaggaacaa ctgacttcac
     2581 gcatcggcag tgatgtgttg ctctgcccgc tgtatggcgc gttgtcgctg aacgatcagc
     2641 gaaaagcgat cctcccggca ccgcaaggga tgcgcaaagt ggtgctggcg accaatattg
     2701 ctgaaacaag tttaaccatc gaaggtattc gtctggtggt ggattgtgcc caggaaaggg
     2761 tggcgcgttt tgatccgcgt acagggctta cgcggttgat tactcaacgc gttagtcagg
     2821 cgtcgatgac gcaacgtgcc gggcgtgccg ggcgtctgga gccgggtatc tgcctgcatt
     2881 taatcgccaa agaacaagca gaacgcgctg ccgcgcaaag tgaaccggag atcttacaaa
     2941 gcgatctttc cggtttgctg atggaattac tgcaatgggg atgcggcgat ccggcgcaga
     3001 tgagctggct ggatcaaccg ccaacggtga atctactggc cgcgaaacgc ctgttacaga
     3061 tgctgggggc gctggatggc gaacgcctaa gcgcgcaagg gcaaaaaatg gcagcgctgg
     3121 gtaacgatcc gcgtttagcg gcaatgctgg ttagcgcgaa gaacgacgac gaagctgcta
     3181 ccgcggcaaa aattgccgcc attctcgaag agccgccacg gatgggcaat agtgacctgg
     3241 gcgtggcgtt ttcgcgcaat caacccgcct ggcagcaacg tagtcagcaa ctgttaaaac
     3301 gcttaaacgt acgcggcggt gaggcagaca gttcgcttat cgcgccgcta cttgccagag
     3361 cgtatgccga tcgcattgct cgtcgccgtg ggcaagatga acgctatcaa ctggcgaacg
     3421 gcatgggggc gatgctcgat gccgacgatg cgctaagccg ccacgaatgg ttgatcgcac
     3481 cgttattatt gcagggcagc gcctcgccgg atgcgcggat tttactggcg ctgccggtcg
     3541 atattgatga gttagtacaa cgctgcccgc agctggtaca gcagtctgac actgtggagt
     3601 gggatgacgc gcaaggtacg ctgaaagcct ggcgtcggct gcaaattgg
//
LOCUS       DAWDRC010000409         3646 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10260_length_3646_cov_1.724311, whole genome shotgun sequence.
ACCESSION   DAWDRC010000409 DAWDRC010000000
VERSION     DAWDRC010000409.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10260_length_3646_cov_1.724311"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2112..2121
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          915 a          921 c          847 g          953 t
ORIGIN      
        1 ttaacaatcg tgccttcagg ttttcctatt tgaggatgcc ggcgcgtcag tattttttta
       61 tttagtatta taacgttata agaattacag cgatgaattc ccgacaacaa actattctac
      121 agatggtcat tgaccagggt caggttagcg taaccgatct ggcaaaagcc actggagttt
      181 ctgaagttac cattcgccag gatctcaaca ccctcgaaaa actgagttac ctccgacgtg
      241 cacatggttt tgcagtttcg cttgatagtg atgacgtcga aacccgtatg atgagcaact
      301 atacgctgaa gcgtgaactc gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca
      361 tctttatcga aaatggtagc agcaatgccc tgctcgccag gactctgggc gagcagaaga
      421 aaaatgtcac tatcatcacg gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt
      481 gtgaagtcat tttgctcggt ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt
      541 tgacacgcca gtgcatccaa caagtgcatt tcagcaaagc atttattggt attgatggct
      601 ggcaacctga aactgggttt accggtcgcg atatgatgcg taccgatgtg gtcaataccg
      661 tgctggaaaa agagtgcgag gcgatagtcc tgaccgacag ctcgaaattt ggtgctgtac
      721 attcatactc catcggcccc gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac
      781 gcgccagcga tctgatgcat cttgagcaca gtaaactcac cgttcacgtc gttgacattt
      841 aaccatttcc cttctctacg gatgatttgc agtttggcaa atcatccgct ctaagatgat
      901 tcctggttga taattaagac tatttacctg ttattaacac tctcaagata taaaattatt
      961 atcagcgata taacaggaag tcattatcac ctgcgtgata taaccctgcg cgcgagcgga
     1021 tttcacggaa taatttcacc tggcttattc ttagctatta tagttataga gagcttactt
     1081 ccgtgaatca taaattcagg agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg
     1141 gctgttctgg caattaccct gggtgcctgt tctaactggt ctaaacggga ccgcaacacc
     1201 gcaatcggtg caggtgcagg ggcattaggc ggtgcagtac tgaccgatgg cagtacgttg
     1261 ggtacattag gtggtgcagc cgtcggtggt gttattggtc accaggtcgg taaataatcg
     1321 taaataaatg tcgattatcg actcgcaatt ataatacgct tgattctgtt cagtacgaaa
     1381 taactgaata atacacacag taaaatattc gaaagccacg gatatatccg tggcttttta
     1441 tgttagccgc ctgcgagttt taccttcatc cctttcgctt caagcattga ttttaataaa
     1501 tcacgcttat cgccctgaat ttcaataatt ccatctttaa ctgctccgcc gcagccgcat
     1561 tttttcttca gttcagctgc cagtttcgtc agttcggcat catcgagatc gacaccggta
     1621 atcaggcaaa cgcccttacc tttgcgtcca ctggtctgac gctgaatacg caccacaccg
     1681 tcgcctttag ggcgtacagg ggccgctttt ggctcatcaa tacgtccggt ttccgttgag
     1741 tagaccagac ggctgttgga atcactcatc atgcactccg ctgtaaagag gcgttgatcg
     1801 ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg gcgaccaatc accatataat
     1861 caacaccagc cgccaacgcc tgttctggcg tcataatgcg acgctggtca ccagcatcac
     1921 tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc ctggccgaat acctgtttaa
     1981 agcgcacagc ttcctgagca gaacacacca caccatcaag gccacatttt tgcgtcagtg
     2041 ccgccagacg ttctgcataa tctgcaggtg acagtgtcac gccaagatcg gccaggtcgc
     2101 tggcttccat gnnnnnnnnn nttcaggtca agaaaaatat caaaaccacg ctgttgaagt
     2161 tcgctcacaa actgtggccc aaacaatgta aacatctctt tgccgacctt cagacgacaa
     2221 tcgcgtgggt cgatcttgtc gacaaaggcc atcgcggcat cgcgattatg ataatcaagg
     2281 gcaacaacta caggagaatt cgtaacagcg cgggaagaag atgaagcagt taacgtcatg
     2341 accagacctt cttgatgatg ggcaccggaa gtggcgcgaa acaggtaaac ggcgagcatt
     2401 ctacccgtgg aaataaaaaa ttaacagttg cgatttctcc tctggcaggc ttttccgcgc
     2461 cgtcggtgcc ggagcacaat agaacatcaa taattagtat gttgtaacta aagtcgggtt
     2521 tttttaaaaa ttacaggcca tcaagaccgc gaattggttt aatggttgac caggcccgac
     2581 aagacggaca atgccagtag agggtgtatg cggtaaaacc acatttctgg cagcgataac
     2641 ggggcttact acgtaccttc tcgccaacca tgtcacgcag caccatcaga ctctctttgg
     2701 cacgcccttc ttctgcttca tttaagtggt aatccattaa cttatggaac acacgcatgg
     2761 tcggatgacg ctgaagctgg cgagtaatat agacctgtgc ggcctcacta ccgtcgcgcg
     2821 cttcaatgat atccgcaagc atcaattcag catcggcacc ggtgttctct tccaccgcgc
     2881 gctgtaggaa ttctgcccat tcggcagttt tacccaactg ctgatagcat gtttgcagca
     2941 tttccagcgt ttcgctgacc agttctctgt cctgggatat gacacgttgc aaactttcga
     3001 cggctttggc gtattctcct ttcgccataa acacgcgtcc catcatgatg gatacgcggg
     3061 cgctgttttt atctgccgcc gcaccttttt ttagcaaagt catggcacga tcgagatcgt
     3121 cgctggccat atgctgcaga gctaactcac agtagaaatg ggcaatttcg acgcgctgtt
     3181 tatctttacc cagcttcacc aggcgttcgg caacatcaat tgctttctgc cactcgctgg
     3241 tagcctggta aatttgtagc agttgttgca gcgcgccaat gcggaagtca gtttcatcgg
     3301 tcagctgatt gaacatgtct tccgcgcggt cgtataaccc ggcagccatg taatcacgcc
     3361 ccagttgttg aatcgccaaa agccgctgtt cataggtcag cgaggcgctt tccattaggg
     3421 tctgatggat gcgaatagcg cgatcaactt cgccacgcga acggaacagg tttccgagcg
     3481 taaggtgggc ttcaacggta cctgtatcct ctttaagcat atcgagaaac aggtctaccg
     3541 ctttatcctg ttgattacta agcaggaagt taacccccgc tacgtaatca cgcgacaagc
     3601 ggttggcttc atcttgcttg ttttgttgcg cacttctgcg gcccat
//
LOCUS       DAWDRC010000410         3640 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10285_length_3640_cov_2.689261, whole genome shotgun sequence.
ACCESSION   DAWDRC010000410 DAWDRC010000000
VERSION     DAWDRC010000410.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10285_length_3640_cov_2.689261"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1081 a          810 c          802 g          947 t
ORIGIN      
        1 attctgtaag gaatggttta tgttttttaa tactaaacat accgcagcat tgtgcttagc
       61 gagctgtatg gcttttagct catcagctat tgcagacatc gtaatttcag gtacacgcgt
      121 agtttataaa agcgaacaaa aaagcgtcaa tgtacgtctg gaaaataaag gcaataaccc
      181 attgctggtt cagagttggt tagatactgg agatgataac gctgaaccag gcagtattac
      241 tgtccccttt accgctacgc cgccagtatc acgtattgat gctaaacgtg gccaaacaat
      301 caaactgatg tacaccgcca gcagtgctct gccaaaagac agagaaagtg tgttctggtt
      361 taacgtactg gaagtacctc caaaaccaga tgcagcaaaa gcagcaaacc aaagcctgct
      421 acaactggca tttcgcacac gtataaaact gttctatcgc ccggaaggat tgagtggaat
      481 ttcatctgat gcaccattgg ctctgaagtg gtcctgggca acatctgaag gcaaagcagc
      541 attacgcgtt gataacccta cgccctacta tgtctctttt agcagtggtg atttagaagc
      601 caacggcaaa cgctaccccc tggatatggc aatgattgcg ccgttcagta acgacgtcat
      661 caaggtaaat gggatgagtg gcagaacaag ctctgcaaaa gtgcattttt tcgccattaa
      721 tgattttggt ggatctattg aaggcaacgc caacctttaa tctggcaagg atataacaca
      781 gcaggaagca gaacgtgact attaaatcta ctaatcacct tacccacatc gccacgtttt
      841 gcgctctgct gtatagcaat agtgctttat gcgctgaact cgtcgaatat gaccatacat
      901 ttctgatggg aaaggatgct tcaaatatcg accttagtcg gtatacagaa ggcaatccca
      961 cactacctgg catttacgat gtcagtgtgt atgttaacga tcaacccatc atgagccaaa
     1021 gcattgcttt tgctgttatt gaaggtaaaa agaacgctca ggcctgcatt acgcagaaaa
     1081 atttattgca atttcatatc agttcaccag acaagaatag cgaaaaagcc attttgctta
     1141 agcgagacga cgatcttggt gattgcctca atctggctga aatgatccct caatcatcca
     1201 ttcgttacga tgttaacgat caacgcctgg atatcgatgt cccccaggca tggattatga
     1261 agaattatca aaactatgtt gatccctctt tgtgggaaaa tggcattaat gcagccatgc
     1321 tttcctacaa cctcaacgga tatcacagtg aaagtccagg cagaacaaat gacagtatct
     1381 atgcggcatt caacggtggg ataaacttag gcgcgtggcg gttacgtgcc tcaggtaact
     1441 acaactggat gaccaatgtc catagcgatt acgattttca gaatcgctat ttacaacgcg
     1501 atcttgcgac actacgttca caactggtta ttggtgaatc ttacacgacc ggcgagacct
     1561 tcgattcagt cagcattcgc ggtattcgct tatacagtga cagtcgaatg ctgcctccgg
     1621 tattagccag ttttgcgcca atcattcatg gcgtagcgaa taccaatgcc aaagtaaccg
     1681 ttatgcaaaa tggttataaa atctatgaaa cgacggtgcc gccaggggcc tttgccattg
     1741 acgatcttag cccgtcaggt tatggcagcg atctcattgt gactatcgaa gaagctgatg
     1801 gcacaaaacg gacgttctcc cagccatttt catctgttgt gcaaatgctg cgccctggcg
     1861 ttggtcgctg ggatattagc gccggtcagg ttttaaaaga cagtatccag gatgagccta
     1921 atttatttca agccagctac tactatggtt tgaataacta cctgacagga tataccggta
     1981 ttcagctaac tgataataac tataccgccg gattgctggg gcttggtatg aatacccccg
     2041 tgggggcttt ttctgtcgat gttactcact caaatgtcag cattccggac gataaaacct
     2101 atcaaggaca aagctaccgt atttcctgga acaagttatt tgaaaatacc agtacatcat
     2161 taaatatcgc ggcctatcgc tactccaccc agcattatct gggcctcaat gatgcgctga
     2221 cgctgattga tgaagtagaa catcctgagc aagatcttga gcctaaatcc atgcgcaatt
     2281 actcgcgcat gaaaaatcag gtcacggtca gtatcaacca accgctgaaa tttgagaaaa
     2341 aggattacgg ttcattttat ctctcgggaa gctggtcaga ttactgggcc tccgggcaaa
     2401 acagtactaa ttactctatt ggttatagca atagtgcgtc ctggggcagt tacagtatca
     2461 gtgctcaacg ttcgttgaat gaagatggtc aaaccgacga tagcatttat ctcagcttca
     2521 caattccaat agaaaactta ctgggtacgg aacaccgcag ctccggcttc cagagtattg
     2581 atacacaatt aaacagcgac tttaagggta ataaccaact caatatcagc agtagtggtt
     2641 acagcgatac caatcgcatc agctacagcg taaacacggg ttatatgatg aataaatcca
     2701 gtgatgattt gagttatatc ggcggttatg ccagttatga atcgccgtgg ggaacgctgt
     2761 caggttcagc ctcagcaagc agtgataata gtcgccaatt ctcactcaat actgatggtg
     2821 gatttgtgtt acatagcggc gggctgacgt tcagtaatga cagttttagt gattccgaca
     2881 ctctggctgt cattcaggcg ccaggagcaa aaggcgctcg tattaactac ggaaacagca
     2941 ccgtagaccg ctggggttat ggtgtgacca gcgccctttc gccataccat gaaaatcgta
     3001 tcgcactgga cattaacgat ctggaaaacg atgttgaatt gaaaagcacc agtactgtcg
     3061 ccgtcccgcg ccagggagcc gtcgtctttg ctgattttga aacagttcag ggacaatcgg
     3121 caattatgaa catcgtgcga agcgatggta aaaatattcc atttgcagcg gatatttatg
     3181 atgagcaaaa caatatcatc ggcaacgtag gtcagggtgg tcaggccttt gttcgcggta
     3241 tcgggcagga aggaaatata cgaattacct ggattgaaga gggcaaaccg gtcagttgtt
     3301 ttgctcatta tcaacaaaac acgacatcag aaaaaatagc acaatctatt attctgaacg
     3361 gacttcgttg tcagatacag taaccacaag ggacttccaa tgataaaaat cacaccacat
     3421 aaaataacaa tactcatggg actattgtta tcgccttcgg tatttgcaac ggatgttaat
     3481 gtcgatttta ccgctaccgt gaaagcaaca acctgtaata ttacacttac cggaactaat
     3541 gttactgata atggaaatga taagtacacc ttagttattc cctcgatggg aatggataaa
     3601 atagccaata aaacagcaca atctgaagct aacttcaaat
//
LOCUS       DAWDRC010000411         3639 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10290_length_3639_cov_2.054129, whole genome shotgun sequence.
ACCESSION   DAWDRC010000411 DAWDRC010000000
VERSION     DAWDRC010000411.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10290_length_3639_cov_2.054129"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2409..2418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3095..3107
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          997 a          943 c          840 g          836 t
ORIGIN      
        1 tgatccaggt gagtaagcca acgatgacgc ctacagcgag acggctaccc atccctggcg
       61 agcgcgggac actaaaggta aacggcacag ccagcaaaat catcgccaga gtcaagatcg
      121 gttgccccag tttttcccac agtgcaaggt ggtattcgct gctgggttgc ccggtatcct
      181 gcaaataatg gatgtaatga ttaagctgac ggacagaaaa agtattcccc ggcatcgata
      241 actcttcaag attcatactg gtgaaggccg attgccaggc aagattatct aatgtttcca
      301 gcgtttcttt gccattcaac cattttttat aattcacacc atgcaggatc cacgttttat
      361 cgtcttcaat ggtggcactg cgcgcataaa tgtaggattc gagcgagaga tcgtcgcggt
      421 aatgaaaaat ctccacgccc accggctggc cctgctcgtt cagcgatttc accgtcacaa
      481 attcattacc gcgcctggcc caaagcatat tgccgataat gtcattgtcc tcccccaacg
      541 cggtggcagt cgatttgatt tgcagcgcct gttgctgcaa tggtgacgcc acccactcat
      601 caatcgcgcc taacgaaaca gtccacagta tccctgcaac cagcgccacc agtgcaatgc
      661 ggaagataga aaaccccgtg ctgcgaatgg cggtaagctc actgtttttc gataactgcc
      721 cgagaccaac aatccccccc aataacgcga taaacggcga aagttcgacc agtgtgcgcg
      781 gtaaggtcat cagcaccacc agcactgcct gagtccagcg ataaccgccc ggactgacat
      841 catccagttc gttaatcagg ttgaaggtgg taaaaagcgg cagcaatagc cctgcggcgg
      901 cggcaaaacc gagaaagagg tgacggatta aatagcgact gaaaacattc atcggggaaa
      961 tttctgcagc aaggagaagt cgcgtgcgac cagcatcaac aggcccatta gcatgagtcc
     1021 gggcactaac cacaaaccag gaatgagggg aatcgcgccg ttagccacca gcgtacgaca
     1081 gacgtcgccg ccataaaaaa tggcaacaaa cagcaatgtc agtggcaata acgttgaaaa
     1141 tcgcccttgc cgcggtttta cccgacttaa tgaaatggcc agcagcgcca tcaacaatgc
     1201 tgtcaggcca cggctttgtc gccattgcag ttcggcatga tcggctggaa agacggagcg
     1261 cgccagctct gtgaccgatt ttgctttgcg tttgacgttg gcactttgat ccagcggatt
     1321 gagatgcaat tgcagattac ggtagatctg ttcgttgtcg tcacgcccct gatgatcgag
     1381 aagataggcg gtaccgttat gcaacataac ggtcggtttc tcgggtgatg ggtcaaccac
     1441 atcaaccgaa cgggcgcgga aaatgcgggt tcgattggca gtagaagtat aaatcagcgc
     1501 gtcagtcagg cgattattat cctgatcaac cgtctgcgaa aggatcattc gtccgttatc
     1561 gttagtgtta aatttctttg cccgcaactg acgaacatcc agctccgact gcgattgttg
     1621 ctccagttga taaatctgcg cataagccca gggacgacca tacatcgaca gaagggtgac
     1681 aaaaatactt aacggtatcg ccagataaag aacggctttg tacaaacgtc ccggactgcc
     1741 ccccgccgcg gagatagcgg taatttccga gtcggtatac atttgcccta gcgtcacgcc
     1801 aactgaagca tatagtccaa caggtaacag catctccagt gcaatcagca ctttgtagaa
     1861 aacgatatcc agcacaacat caagagttaa tgtcccattt gccgcttcgg tcagataacg
     1921 ctgtgcggag tagctggcga aaataaagat cagaaaaccg acgataatca gcaccagacg
     1981 gcgcgtacca cgcatgatgt agtgttcaac cagcttcatc tcgctaacgc cctcaatcct
     2041 ttgaattttt tattcattga tcagtttctg cgccagcgca atgacatgat ctacctcttc
     2101 cggtgtcagg ctgccgtcct ttgcccactg aattcgccag gctttattca ataccagaat
     2161 ggttgaactc tgctccggca attgccaggc aacgcgtccc agaccattgc cgtcaataac
     2221 gaattgcgcc cacggatagc ggcgtttatt tttttcgatt ttgccaacga caaaatagcc
     2281 agtaccaaag atggcatcat cggtgttcac aatagtggtg ggctgaaaac gatcctgcga
     2341 aaagtttgcc gcttcaaccg ctttaattaa cagtgagttc tttttcttcg ccgacttacg
     2401 cccggcgann nnnnnnnngc gatatactga ataattcgga tttttcccgt caattgtgag
     2461 ctgttccact tttggtagtg aatttcgtcg tcgctattca gaagcagctc accgccttcc
     2521 tggatataaa taggggtaac cgttttgcca gtgacgaaat tgtgtgcctg cacacccggg
     2581 gctaagagca tgataatcag cgcaataatc aaacgagatg acatcattgc tccggttata
     2641 gcggaaaacg cgaaataagc agttctaaac gctggcgaat atcgctatcc agcgccaccg
     2701 gtgcatcata tgaccgacgg ttatccatat catccgccac catgtcgtcg tgcttatccc
     2761 ccatcagacc attaatggcg gcgagaaaac gggaaatcag taacgcctct ttattttcag
     2821 gatcaagcga tcttaaggtg tcgtaataag aaacctgctg gctgatgatt tttcgcacat
     2881 catgaaattc cctgccagtt agttgcggtt tagcaataat ctctttcagg atgtacattt
     2941 gcttctggcg gtaggcgata aaaccgttca cggtttctaa ctggctatgg cgcaatgaat
     3001 agttcaccag ccaccagaca ggggagctca aataaacacg taacaggtta ccgtaaaacg
     3061 acacaatatt tttcttaccg ttacgaaaac catcnnnnnn nnnnnnngat taaacgcagt
     3121 aactcctgac gattaacacc atccagccag aatttcaggc ataacgcgta attatcatgg
     3181 atgatttcgg tggtacaact tagtgcgata gtcggcggca aactgacctg tgcatcaaca
     3241 acatcatctt cttcaatcgc aacaaataga cgctcgctcg cctcaagcgg gaaatatttt
     3301 ctcatcatca ggatttctct ccggttgagt tataaagcaa ggaatggcgc tgaaaaaatg
     3361 cctttaaggt cggaattagc gagagcacca catccagaca ttgcccaccg aagtaagcaa
     3421 atgcagcccc cagcacgcca aacagatgac agaacatgaa cagcaatacg atgtagccga
     3481 ttgaggcgat ggtttgcgcc acgagaaaag cgcgctgttt tcccgccatc aatagcaatg
     3541 actcctgtgg gaagcccagc atcgaaatca caattgcgcc cagcatcacc tgaatcaggt
     3601 catacgcttc gagatactta acgccaaaca ccagcgaaa
//
LOCUS       DAWDRC010000412         3630 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10327_length_3630_cov_2.552448, whole genome shotgun sequence.
ACCESSION   DAWDRC010000412 DAWDRC010000000
VERSION     DAWDRC010000412.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10327_length_3630_cov_2.552448"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1485..1494
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2994..3003
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          885 a          864 c          932 g          929 t
ORIGIN      
        1 aatgtgcttt tttactatat gtattcattg tgtgaatgat atgtcgcagt aaaacgcact
       61 attcgttaaa tgaatcgcag gggaaagcag ggggttaata ggcaaaagca acattttccc
      121 cgccgccaga agcgacgggg cagagattaa agctcctggt cgaacagctc taaaatcgct
      181 tcgtacagct ctttgacggt gaaaccgtta gcaggggtag taaagatggt gtcatcgcca
      241 gcgatggtgc ccagaatacc ttctgctttg cccagtgagt ccagcaggcg agcaattaac
      301 tgcgccgcac cagggctggt atgaatcacg acaactgcat cgttgtagtc gatatccagt
      361 accagattct tcaatggact ggaggtggtt ggtacaccca gttcagctgg caggcagtaa
      421 accatttcca ttttggcatt gcgtgtacgt accgcaccga acttggtcaa catccgcgag
      481 actttagact gattaatatt gtcaaagcct tgctcctgca acgcggcgac gatttcgccc
      541 tgggagctaa atttctcttc tttaagtaat gctttaaatg ctttaactag ttcttcttgc
      601 ttagccgagc ttcgcataag tcacccgata tggtggttga tacaacatta ttgtgcatac
      661 agatgaattt ttatgcaaac agtcagccct gaagaaggct gaaataatgt tatgaaagag
      721 cgggatttta tcaaatttcg ttattgagaa acatgcctgc gtcacggcct gcaaattctg
      781 cttaaaagta aattaattgt tatcaaattg atattgtttt ggctgagcgg tagggtatat
      841 tgtcaccacc tgttggaatg ttgcgctaat gcataagtga ctgttaatta cgtaagttag
      901 gttcctgatt acggcaatta aatgcataaa cgctaaactt gcgtgactac acattcttga
      961 gatgtggtca ttgtaaacgg caattttgtg gattaaggtc gcggcagcgg agcaacatat
     1021 cttagtttat caatataata aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg
     1081 cggtattggc caggcgcttg cactactgtt aaaaacccaa ctgccttcag gttcagaact
     1141 ctctctgtat gatatcgctc cagtgactcc cggtgtggct gtcgatctga gccatatccc
     1201 tactgctgtg aaaatcaaag gtttttcagg tgaagatgcg actccggcgc tggaaggtgc
     1261 agatgtcgtt cttatctctg caggcgtagc gcgtaaaccg ggtatggatc gttccgacct
     1321 gtttaacgtt aacgccggca tcgtgaaaaa cctggtacag caagttgcga aaacctgccc
     1381 gaaagcgtgc attggtatta tcactaaccc ggttaacact acagttgcga ttgctgctga
     1441 agtgctgaaa aaagccggtg tttatgacaa aaacaaactg ttcgnnnnnn nnnnttcggc
     1501 gttaccacgc tggatatcat tcgttccaac acctttgttg cggaactgaa aggcaaacag
     1561 ccaggcgaag ttgaagtgcc ggttattggt ggtcactctg gtgttaccat tctgccgctg
     1621 ctgtcacagg ttcctggcgt tagttttacc gagcaggaag tggctgatct gaccaaacgt
     1681 atccagaacg cgggtactga ggtggttgaa gcgaaagccg gtggcgggtc tgcaaccctg
     1741 tctatgggcc aggcagctgc acgttttggt ctgtctctgg ttcgcgcact gcagggcgaa
     1801 caaggcgttg tcgaatgtgc ctacgttgaa ggcgacggtc agtacgctcg tttcttctct
     1861 caaccgctgc tgctgggtaa aaacggcgtg gaagagcgta aatctatcgg tactctgagc
     1921 gcatttgaac agaacgcgct ggaaggtatg ctggatacgc tgaagaaaga tatcgccctg
     1981 ggcgaagagt tcgttaataa gtaattgatt agcggataat aaaaaaccgg agcacagact
     2041 ccggtttttg tttttttacg ctgaccttag ttggtcgccg gatattcctg aatggtgacc
     2101 tgcaatgtta actgtttatc gtcacgcatc acgacaaccg ggatcaccga accagggcga
     2161 atttccgcga cctgatccat tgtctccagg gcggaaatag cgggtttgtt attcacagag
     2221 ataatcagat cgttgacctg gatgcctgca tttgccgctg ggccatcagg cgacacttcg
     2281 ttaaccacga tcccttgcag ttgatccatg ccgccactct gcgcatgtaa tggggcgatc
     2341 tcacgtccac caatgccaat gtagccacgg atcacacggc catcacggat cagtttatcc
     2401 ataattttgg tcgctaactg gaaaggaatc gcaaagccga taccttccgg cgtttcgcca
     2461 tcgttactct tatcaaacga cagcgtgtta atgcccatca gttcgcccag cgagttcacc
     2521 agcgcgccgc cagagttacc gtggttaatg gaagcatcgg tttgcaggaa gttttgccgc
     2581 ccggtcgggt tcagaccgat tcgacccgtg gcactgataa tcccctgggt aatggtctgc
     2641 ccgaggttgt acgggttacc gatcgccaga accacatcgc caatgtgcgg tacgcggcgt
     2701 ggattgatcg gaatcgttgg taaaccgcct gtggcgttaa ttttcagcac cgccagatcg
     2761 gttagagagt cagagccaac cagtaacgct tcaaaaacac gtccatcctg taaggcgacg
     2821 atgatctgat cggcgtcgtt gatcacgtgt ttattggtga tgatataccc gcgttgatcc
     2881 atgattacac cagatcccag ggtgcggatc tcaagctggt tgtgagagtt ggtgttcaaa
     2941 ccgcggttat aaacgttaac caccgctggc gcggcgcggc gaaccgccag attnnnnnnn
     3001 nnnctgtcaa attgcggagt ggaaagcggg ttaaggctgc gcagcgaagg catggcaacc
     3061 agcagaatag cgccgacaat taatccaatc gcaacggaac gtaagagctt cacaaacatg
     3121 atggaggcat cgttaaaaaa gggaacggca gcagcatacc acgagttaac cggacatcac
     3181 acgtcagcct gatgcccggt ttacgacatt aacgcattag taggtagagg ctttcattgc
     3241 cgcgtatgat ttgcaaggcg attatggccg gttttgccgc cagcacttta cgcatttcag
     3301 caatcgagtt cacccgatcg cggttgacgc caatgatcac atcgtctttt tgcaagcctg
     3361 cctgagcagc tgggcttcct ttgacaacct catcgatctt aatacctttg ccaccatctt
     3421 ttagctgacc atcgctcaac gttgcacctt ccagcgctgg cgtgatcatt tcagcgctgg
     3481 ccgacgaaga ggtgctggta tcgagcgtca cttctacttc cagtggtttg ccgttacgca
     3541 gcaggccaag cttcactttc gtgcccggct cggtggtcgc gatacgagag cgcaactcag
     3601 caaagctatt cagcggtttg ccgttgaggc
//
LOCUS       DAWDRC010000413         3618 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10359_length_3618_cov_2.007017, whole genome shotgun sequence.
ACCESSION   DAWDRC010000413 DAWDRC010000000
VERSION     DAWDRC010000413.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10359_length_3618_cov_2.007017"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1421..1430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          799 a          997 c          973 g          839 t
ORIGIN      
        1 tttgggcaaa ccagcctgaa ccgttcgggt gccattcgtc gctgtatttg ccgccattaa
       61 agaatgaggc gctggcgcgc agcatggtat cggatgcgcc attttgcccg tttagcggca
      121 actgctgttc gacggcgagg aaaaagatct gatcgcgcag cggcttccag cgcagaccgg
      181 tgccggacat cggatttttc accggcatca tcaccccgtt ttcaccggta tcggcaaaga
      241 cgcggctata aactgagagc aggtcgcctt ccagcagcat attgcgtccg aggcggtact
      301 cggcttccag ttgcccgtag ctacgatagc ttttccctgg cgctgcgccg ccgacattat
      361 tgttagcggt actcattgcg ccggaacgca agccgataga agaatcgaaa ctgaacgtcc
      421 agcggcgacc gacctcctca tgcagacggc ggaaattgaa gcgttgctga ttttgctctg
      481 gggtcagtgg ggttatcagc gcctgattat caatgtcatc aatcaccagt cgggcgtagt
      541 gctgcgtcgc aggcatgtca tccagacgct ggttcacgta ggccagttgt cggataagtg
      601 ccggatcgtc cggtagccct ttatgcgcct gttcgagcat ttcccgcgac tgtgcgatat
      661 caccgctatc ccacaaggcg taaccgagcg ctgcttgggt gttgctatta ttcggttcca
      721 gttccagcgc ggcgcgcaaa tcactcaccg ccgccgggac attatgacgt tggcgataaa
      781 ttgtcgcccg cgcaacgtaa gcgttggcag aaggcgcaat attgattgag cgcgtgagat
      841 cgttcagtgc aagttccggc tgaccaggaa tgtaacgttg cgcatgcagc caccagtaga
      901 gggcattgtt tcccagccca cgttgttctg cctgctgaag ccagcgatcg cgagccgcac
      961 catttcctgc cgcctgggcg gtattggcag cagcaagcag atcctcattg ctcatgtcgt
     1021 gaagactgat tttctgccag gccgccagtg cggtggcgta gtcctcaacc tgatacgcct
     1081 gataggctac cgcacgatgt tgccaggcgt ccggttgtcg ttgttcggcc tgaagccatg
     1141 catacaacgc cacaccgggt agcgtgtccc gataacactt tgccagacgg ttccaggcgg
     1201 cggcatcgta ggaaggcgac atatcgccca gcaagcgaac tattgccggg caattatcag
     1261 caatacccgg caactgactt tgccactgac gttgctccgc cagcggtaaa ggtttcgata
     1321 aaattgccac cttcgccggc gttgccaggt aaggatgact ttccagcaga gacgccagtc
     1381 gcgccattaa agtctggctg acacgcgcat cgccctggaa nnnnnnnnnn cttcagcctt
     1441 gttacgggtt gctacgctaa cggcataacg ctcctcaagc atttcattgg cggggagggt
     1501 ggcgagcagt ttttgcgctg cgccgtactg accttctttt aacagcaccg gtagcgtcgc
     1561 gccaacaaca tactggcggt tgtcggcaaa ctgtaccgta taattcgcca acgcctgaac
     1621 ggggttgccg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     1681 aaatagcggc gtattttcaa tgagataatg ctggaggcgt gctttttcgc cacgataagc
     1741 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     1801 cgccaggatc cgatcgtcca actgcccggc aagaagcacg tcaaaccact gacggcgttc
     1861 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     1921 ttgtttcagg tagattgcgc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     1981 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaattgtg ccagccgcag
     2041 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     2101 ctgggcaagc agttcttcaa cagtcgtaac gcttttcact tcaacaggaa tagccgccag
     2161 actgcgctca agccgatcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     2221 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     2281 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     2341 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     2401 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     2461 taaagatgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     2521 tttcggactc cagttgtgca acctgttcag tgtttaaacc tgctttgagt aatagtgatt
     2581 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     2641 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     2701 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     2761 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     2821 gatgctgaac tgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     2881 agtggcgtaa tccggtgaca atttgtcccc gcagaacaat gacgtaacgc actttgcgtc
     2941 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     3001 ttaactcatc attgtccaga cgcagcggca gtaccgcata atgcaacgcc acggaggccg
     3061 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     3121 cgttttgctc tgccagcgcc tgtgccagtt gctcggcgct aatcagcccc tgcatcagca
     3181 ttgaaccgcc caggcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttctt
     3241 cagtgatgac ctgattttcc agcaggattt gacctaacgg acgcaacgag cgggtatcgc
     3301 cagtcacgct ggggaagtca tgcgttgttt tatcccacgc gacgcgacgt ggatcgccgt
     3361 gttgaagtac ctgttttaac gcgcgccagt tggccatgaa gttaatcagg ttgccccaga
     3421 aaagacgcag gacagatagc agcccctgcg tcaggccgta gtagccagta acgaaaatca
     3481 cccgctgcac gatgcggtta atcatcaagc caaagtttag ccacagcaga gtcattaacc
     3541 atgcgctgcc gctgaaaata gaaaggaaat gccgggcatc gggccacaaa ctttcatacg
     3601 ccagcaacag caaaagct
//
LOCUS       DAWDRC010000414         3616 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10366_length_3616_cov_2.554058, whole genome shotgun sequence.
ACCESSION   DAWDRC010000414 DAWDRC010000000
VERSION     DAWDRC010000414.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10366_length_3616_cov_2.554058"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          912 a          831 c          950 g          923 t
ORIGIN      
        1 caacagtact ataaaatacg ttaatataat gaccaataaa tatttttatc atgaatgttt
       61 tttgcccgta ttgttcgggt taattaatgt tacatattca gcgggctgat tttcattttt
      121 gctgaataaa gtcaattttt gtcacatttc atcgccgggc ttactgtgaa acgatcccgg
      181 aagccgttgg tgacgggcgt gaccataact gtggacaatc gaattgacaa aaacgagaga
      241 aaaatcaaat acccaccatt tttaacgttt caaagttgca ataaaaaccg ctaatatacg
      301 aatgactaac tatcagtagc gttatcccta tttctggaga tattcctttg atcaacgttc
      361 tacttgttga tgaccacgaa ctggtgcgcg cagggatacg acgcattctg gaagatataa
      421 agggtataaa agtcgtcggt gaggcatcgt gcggtgaaga cgccgttaaa tggtgccggg
      481 caaatgccgt tgacgtggtg ctaatggaca tgagtatgcc gggcattggc ggtcttgagg
      541 cgacgcgtaa aatcgcgcgt tccacagctg atgtcaaaat catcatgctt accgtccata
      601 cagaaaaccc tttaccagcg aaagtcatgc aggccggagc tgcgggctac ctcagcaaag
      661 gcgcggctcc gcaggaagtc gtgagtgcga ttcgttctgt ctattcaggg cagcgttaca
      721 ttgcttctga tatcgctcaa caaatggcgt taagccagat cgaaccagaa aaaacagaaa
      781 gcccatttgc cagtttgtct gaacgtgaat tgcagattat gctgatgatc accaagggcc
      841 agaaggtcaa tgagatctca gaacagctca atctcagtcc gaaaacggtg aacagctacc
      901 gctatcgtat gttcagtaaa ctaaacattc atggcgatgt tgagctgact cacctggcaa
      961 ttcgccatgg tctgtgtaat gcggagacat tatcaagtca gtgagtgatc agtttgacgc
     1021 aaaagcgttt ttaaaaaccg taaccagcca gccaggcgtt tatcgcatgt atgatgctgg
     1081 tggtacggtt atctatgtcg gcaaagcgaa agacctgaaa aaacggcttt ccagctattt
     1141 ccgtagcaac ctcgcttcgc gcaaaaccga agcgctggtc gcccagatcc agcaaattga
     1201 tgtaacggtt actcatacag aaaccgaagc gctgttactg gaacacaact acatcaaact
     1261 ttatcagccg cgttacaacg ttttgctacg cgatgataaa tcatatcctt ttatcttcct
     1321 gagtggcgat acccacccgc gtctggcgat gcatcgtgga gcgaagcatg ccaaaggtga
     1381 atatttcggc ccgttcccga atggctatgc cgtacgtgaa acactggcgc tactgcaaaa
     1441 gatttttccc attcgccagt gcgaaaacag tgtttatcgc aatcgctcgc gtccgtgtct
     1501 gcaatatcag atagggcgct gtctggggcc gtgcgttgaa ggactggtga gtgaagaaga
     1561 atacgctcag caggtcgagt atgtgcgctt gtttttgtct ggcaaagatg atcaggtgct
     1621 tacgcaactc attagtcgta tggaaactgc cagccagaat ctggagtttg aagaagcggc
     1681 gcggattcgc gaccaaattc aggcggtgcg acgtgtcacc gaaaaacagt ttgtttccaa
     1741 taccggcgac gaccttgacg ttattggagt ggcgttcgat gcgggtatgg cttgtgtcca
     1801 cgtattgttc attcgtcagg gcaaagtgct cggcagccgc agctatttcc cgaaagtgcc
     1861 tggcggtacg gaactgagcg aggtggtgga aaccttcgta ggtcagttct atttacaagg
     1921 cagccagatg cgcaccttac cgggtgagat cctgctcgat tttaatctta gcgataaaac
     1981 gctgctcgcc gattcccttt cagaactggc gggacgcaag attaatgttc aaaccaaacc
     2041 tcgtggcgat agagcgcgtt atctgaaact cgcgcgtacc aatgcggcga cggccttaac
     2101 cagcaaactt tcgcagcaat ctaccgttca ccagcggctg accgcgcttg ccagcgtgtt
     2161 gaaattgccg gaagtgaagc ggatggagtg ctttgacatc agccatacca tgggggaaca
     2221 aaccgtcgct tcctgtgtgg tgtttgatgc caacggcccg ctgcgtgcgg agtatcggcg
     2281 ctataacatt actggcatca cgccgggcga tgattacgcg gcgatgaatc aggtgctgcg
     2341 tcggcgttat ggtaaagcta tcgacgacag taagatcccg gatgtgatcc ttattgacgg
     2401 cggcaaaggc cagcttgcgc aggcgaaaaa tgttttcgcc gaactggatg tctcatggga
     2461 taaaaatcat ccgctgctac ttggtgttgc caaaggagca gatcgtaagg ctgggctgga
     2521 aacgctgttc tttgagccgg aaggtgaggg atttagtttg ccgccagatt ctcccgcgct
     2581 gcatgttatc cagcatattc gcgatgaatc acacgatcac gcgattggcg ggcaccgtaa
     2641 aaaacgggcg aaggtcaaaa ataccagttc cctggaaacc attgaaggcg tcgggccaaa
     2701 acgtcggcaa atgttgttga aatatatggg cggtttgcaa ggtttacgta acgccagcgt
     2761 cgaggaaatt gcaaaagtgc cgggtatttc gcaaggtctg gcagaaaaga tcttctggtc
     2821 gttgaaacat tgatgtctct gtagcaacat aggggtaatc ttactgacaa cagatagtta
     2881 cccgtcatta tgcaatttaa tatccctacg ttgcttacac tgttccgtgt catccttatc
     2941 ccattctttg tattggtctt ttatctgcct gtcacctggt cgccgtttgc cgccgcgctc
     3001 attttctgcg tcgcggcggt gactgactgg ttcgatggtt ttctggcacg ccgctggaac
     3061 cagagtaccc ggtttggtgc tttccttgac cctgtcgcag ataaagttct cgtggctatc
     3121 gccatggtgc tggtaaccga gcattaccac agctggtggg tgaccttacc ggcagcaacg
     3181 atgatcgccc gtgaaattat tatttctgcg ctacgcgaat ggatggcgga gttgggtaaa
     3241 cgcagtagcg tggctgtctc ctggattggg aaagtgaaaa ccactgccca gatggtggcg
     3301 ttagcctggc tgctgtggcg tccgaacatt tgggttgagt acgccggtat tgcacttttc
     3361 tttgtggctg cggtactgac tctgtggtca atgttgcaat atttgagcgc tgcgcgtgca
     3421 gatttgcttg atcagtgatc gtttcggcgc aattttcagc aaacgatcaa aagtggtgaa
     3481 aaatatcgtt gactcatcgc gccaggtaag tagaatgcaa cgcatcgaac ggcggcactg
     3541 attgccagac gataataaaa tcaagtgatt aactgattgc ttgatgaatg cgggaatagc
     3601 tcagttggta gagcac
//
LOCUS       DAWDRC010000415         3609 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10386_length_3609_cov_2.249578, whole genome shotgun sequence.
ACCESSION   DAWDRC010000415 DAWDRC010000000
VERSION     DAWDRC010000415.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10386_length_3609_cov_2.249578"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2278..2287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          849 a         1035 c          863 g          852 t
ORIGIN      
        1 ctgcccgagg atcagattcg ggtcattgat aatactgtga tcaaaatgca gccctgcgga
       61 gagcatgccc gacaatcgac gggccagcga cacaccgcca aaccagataa cactaacgcc
      121 caccagcaaa atcagcagtg aagtcagttc acgggaacgc gggatttgcc cctcttcccg
      181 cgctttttct agtcggtgag gtgtgggggc ttctgttttg tcgtcgctct cgtcagacac
      241 gtcgccaatc ctggaaagag tattaagcgt aaatgatgcc agagcgcaaa gcgttcaatg
      301 gtttgagtaa ggggcaaaac aggcgggatt tagggctttt gccacagcac atcaggcatg
      361 gtgacatgtt tgtcgggtac ggcgtaaacg ccttatccaa ccttgaagat ctgatgtgac
      421 gcggccacgt cacatcaggc aatacaaatc aaaatccaag actatccaac aaatcgtcca
      481 cctgatcctg actggctacc acaccggctt tgctggtatc gacctgaggt ccattaagca
      541 aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc aacagcacca
      601 tcagcaactg acgttcgatc tcctgaatga catccatcat ccgcttaatg acctgtccgg
      661 tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta gtaaagctgg
      721 tatgcgcggg tacatctgcc agaaattgcc gcgtatctgt taccagttca cgggcgtcgg
      781 caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct tttgctgatt
      841 tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc cgctccgcag
      901 cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg atagcttccg
      961 cggcttcggc aatggcctga tccagcccca gttcccgcaa actgtcgcgc agcatacgcg
     1021 tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca ggtttgattg
     1081 atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt gttgagtttt
     1141 tcctccagcg tcgcggcggt aaatggtttc accacatagc cactggcccc cgcttgcgcc
     1201 gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg caatgccgac
     1261 atcgcgccat ccgcacgaat cgttttcagc aactccaggc catccatatt gggcatgttc
     1321 cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc gtcgaggcca
     1381 tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg cactatgcgt
     1441 cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc catttcacac
     1501 tcctgattta aatacgtatc gcctgtccgg cactaatttt tgccagcatt tgctgactta
     1561 cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg cgcggcatgc
     1621 cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc tgacgcatcg
     1681 ccaacattcc cgccgcgccg tcgttgccca taccggttag gattacccca accgcattac
     1741 gccccgcctg tttggccaca gaatggaaca acacatctac cgatggccga tggcgattaa
     1801 ccgccgggcc atcgtgaatt ttgatttgat aatttgcgcc actacgcgcc agctccatat
     1861 gccgatcgcc cggcgcgata taggcatgtc ccggcaagac gcgctctccg tcttctgctt
     1921 ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg gtgaaaccgg
     1981 gcggcatatg ctgggtaatt aacagtgccg gactggaaag cggcaacggt tgcagtacgt
     2041 gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt tcagaactca
     2101 acaacggccc tgccttcagc gttgtcggta ccgacaatgg cttatgtgct gcaaggctcg
     2161 cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atatgccagc attccttcgc
     2221 gaatacccag ttgcggtttg gtgacaaaat ctatcgcccc caactccagc gcgcgcannn
     2281 nnnnnnntgt cgctatggct gttgatgatt tctgtcatga tctggcgcat cagtgccgaa
     2341 tcatcgacag ataacaccct gattttgctc atcgttaatc cttacttagc gcatacaccg
     2401 tctgaccacg cagcgtgaag cggcgctcaa ggtggctaaa gttttcagag tgacccgcaa
     2461 acagcaatcc gtcgggttta aggagcgaaa caaagcggcg caaaatctcc tgctgggtag
     2521 tttgatcgaa gtagatcatg acgttacgac agaagatcgc atcaaacggc cccggcacgg
     2581 tgtactgttt cgccagcaaa ttcagcgggg caaaatcaac atagttcgcc agctcctgac
     2641 gcacgcgtac cagcccttca tgcggtcccg tccctcgcat gaaataccgt tgcagttgct
     2701 gcggcgtcag gtttttcaac tcttcatggc gataaatacc gcttctggct ttttccagca
     2761 cttcggtgtc gatatcgctg gcaaacactt tccagcgtcc gggcgcggtg cccaatgtat
     2821 cagccagcgt catcgctatg ctgtacggct cttcgccggt tgaagccgcc gcgctccata
     2881 cgcgatactc gccagaacgg cgacgtgcgt gatccgcgag cagaggaaaa tggtgtgcct
     2941 cacggaaaaa tgccgtcaga ttcgtggtca gcgaattgat aaacgcctgc cactcaccgc
     3001 tgtgctgatt agattccagc aagttcaggt aatgaccgaa atccgccagt cccagcgcac
     3061 gcaaacgacg aaccagtcgg ttgtaaacca tgtcgcgttt atggtcagcc agaacgatcc
     3121 cggctcgttg atagatcaat tgacttatcc gccgaaaatg cgcgtcggaa agcgccaggc
     3181 gctcggtcat ctgtaacaat aaagacgttt gcccacaggg cagagatgaa gtcatagcgc
     3241 cttctcaatc acttcaggat accactggcg caatttgtaa ctgcgccgac tcatgtcgtg
     3301 ctacttcatg ttcttcaagg gtaaataccg ccacacgcga cgaaagatgg tcggcctgat
     3361 tcagtcgttg caaatcgtcg cgaaatgccc aaaagacatg ccgttactgc caatctgcaa
     3421 gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac gattaaacat
     3481 caacgctccc catcaggcgg caatgaccgc tttagtaaat actcgtcaaa atgtttccca
     3541 gtttggatct tgttcagcaa ttcgcagtcg tggctgtgcc ggtggttgct cactggcagg
     3601 acgggatgg
//
LOCUS       DAWDRC010000416         3600 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10405_length_3600_cov_2.051622, whole genome shotgun sequence.
ACCESSION   DAWDRC010000416 DAWDRC010000000
VERSION     DAWDRC010000416.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10405_length_3600_cov_2.051622"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    655..664
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1855..1864
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          875 a          910 c          919 g          876 t
ORIGIN      
        1 ggggaccact caagttatca acagcaaaac tccgctgaaa agttacccgc tggacatcca
       61 caacgttcag gatcacctga aagaactggc tgaccgttac gcaatcgtcg ctaatgacgt
      121 acgcaaagcg attggcgaag cgaaagatga cgacaccgca gatatcctga ccgccgcatc
      181 tcgcgacctg gataaattcc tgtggtttat cgagtctaac atcgaataaa tccatcgctg
      241 atggtgcaga actttagtac ccgattaaag cggcttcctg acaggaggcc gttttgtttt
      301 gcagcctgcc tcaacgcact tatttagtgc atccttctgc tatccccagc tgattaagta
      361 aattttttgt atccacatca tcacacaatc gttacataaa gattgttttt tcatcaggtt
      421 ttacgctaaa taatcactgt gttgagtgca caattttagc gcaccagttt ggtgccccag
      481 aatggtgcat cttcagggta ttgccctata aatcgtgcat cacgtttctg ccgcatctcg
      541 aaaaatcaat gagttgcaaa actggcacga ttttttcata tatgtgaatg tcacgcaggg
      601 gatcgtcccg tggatagaaa aaaggaaatg ctatgaagtc tgtattaaaa gtttnnnnnn
      661 nnnntggctg cactgaccct ggcttttgcg gtttcttctc atgccgcgga taaaaaatta
      721 gttgtcgcga cggataccgc cttggttccg tttgaattta aacagggcga taaatacgtg
      781 ggctttgacg ttgatctgtg ggctgccatc gctaaagaac tgaagctgga ttatgaactg
      841 aagccgatgg atttcagtgg gatcattcca gcactgcaaa ccaaaaacgt cgatctggcg
      901 ctggcgggca ttaccatcac cgacgagcgt aaaaaagcga tcgatttctc tgacggctac
      961 tacaaaagcg gcctgttagt gatggtgaaa gctaacaata acgatgtgaa aagcgtgaaa
     1021 gatctcgacg ggaaagtggt tgctgtgaag agcggcactg gctccgttga ttacgcgaaa
     1081 gcaaacatca aaactaaaga tctgcgtcag ttcccgaaca tcgataacgc ctatatggaa
     1141 ctgggcacca accgcgcaga cgccgttctg cacgatacgc caaacattct gtacttcatc
     1201 aaaaccgccg gtaacggtca gttcaaagcg gtaggtgact ctctggaagc acagcaatac
     1261 ggtattgcgt tcccgaaagg tagcgacgag ctgcgtgaca aaatcaacgg cgcgttgaaa
     1321 accctgcgcg agaacggaac ttacaacgaa atctacaaaa aatggttcgg tactgaaccg
     1381 aaataataac gctgcgcctg taaagcgcac tggcagttcc ctctccccgc cggggagagg
     1441 attagggtga agggcgcaaa cccgctccgg ggccattaat taccctgaat ttgattattt
     1501 acaccacggt aacaggaaca acatatgcag tttgaatgga gtgccatctg gcctgccatt
     1561 ccgcttctga ttgaaggtgc caaaatgacc ctgtggattt cggtcctcgg tctggctggc
     1621 ggtctggtga tcggattgtt ggcaggtttt gcacgcacct tcggtggttg gatagccaac
     1681 cacgtcgcgc tggtctttat tgaagtgatc cgcggcacac ctatcgtcgt ccaggtgatg
     1741 tttatctatt tcgccctgcc gatggcgttt aacgatttac gcatcgaccc gtttactgcg
     1801 gcggtggtca ccatcatgat caactccggc gcgtacattg cggaaatcac gcgtnnnnnn
     1861 nnnngtctat tcacaaaggt tttcgtgaag caggactggc gctcggtctt tcacgttggg
     1921 aaaccattcg ctacgtcatt ttaccgctgg cactgcgtcg tatgctgccg ccgctgggta
     1981 accagtggat catcagcatt aaagacactt cgctgtttat tgttatcggc gtggcggaac
     2041 tgacccgtca ggggcaagaa attattgccg gtaacttccg cgcccttgag atctggagcg
     2101 ccgtggcggt gttctatctg attattaccc tggtgctgag ctttattctg cgtcgtctgg
     2161 aaagaaggat gaaaatcctg tgattgaatt taaaaacgtc tccaagcatt ttggtccaac
     2221 ccaggtgctg cacaatatcg atttgaacat tgctcagggc gaagtcgtgg tgattatcgg
     2281 gccgtccggt tccggtaaat cgaccctgct gcgctgcatc aacaaactgg aagaaatcac
     2341 ctccggcgat ctgattgtcg atggcctgaa ggttaacgat ccgaaagttg atgagcgcct
     2401 gattcgccag gaagcgggta tggtgttcca gcagttttac ctcttcccgc atctgacggc
     2461 gctggaaaac gtcatgtttg gcccgctacg cgtgcgtggc gcgaataaag aagaggcgga
     2521 aaaactggcg cgtgagctgc tggcgaaagt cggtctggca gagcgtgcac ataactaccc
     2581 ttccgaactt tctggtggtc aacagcagcg tgtggcgatt gcccgcgcgc tggcggtgaa
     2641 gccgaaaatg atgctgtttg atgaaccgac ttccgctctt gacccggaac tgcgccatga
     2701 agtgctgaag gttatgcagg atctggctga agaagggatg acgatggtga tcgtgaccca
     2761 cgaaatcggt tttgccgaga aagtagcttc gcgcctgatc tttatcgaca aaggccggat
     2821 tgcagaagat ggcgatccgc aggtgttgat caagaacccg ccgagccagc gcttgcagga
     2881 atttttgcag cacgtctctt aacttctctg cctccctttc ccccgaaagg gaggcacacc
     2941 agattcctct catttaaaat cgcccagcct ccagcatcta tacttatctt tttgctctgt
     3001 tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct gttgggcgca
     3061 cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga ctcaacgact
     3121 gaaccggccc cggaaccgga tatcgaacaa aaaaaagcgg cctatggcgc actggcggat
     3181 gtgctggata atgacacctc gcgtaaagag ttgatcgacc agttgcgcac cgtcgccgcc
     3241 acgcctcctg cggaacccgt accgaagatc gtgccgccga cgctggttga agagcaaacc
     3301 gtgctgcaaa aggtcaccga agtcagccgc cattatggtg aagccctttc cgcccgcttc
     3361 gggcaacttt atcgcaatat caccggctcc ccgcataagc cgtttaatcc acaaaccttc
     3421 agcaatgcgc tgacccactt ttcaatgtta gcggtattag tgtttggttt ttactggctg
     3481 attcgtctgt gcgcactgcc gctgtatcgc aaaatgggcc agtgggcgcg gcaaaaaaat
     3541 cgtgagcgca gtaactggtt gcagcttccg gcgatgatta tcggggcgtt tattatcgac
//
LOCUS       DAWDRC010000417         3596 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10415_length_3596_cov_2.768992, whole genome shotgun sequence.
ACCESSION   DAWDRC010000417 DAWDRC010000000
VERSION     DAWDRC010000417.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10415_length_3596_cov_2.768992"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT         1016 a          865 c          804 g          911 t
ORIGIN      
        1 tacgccagcc aatatgcccc acatcatgga gacagcaccc aaaacgtcag ctcctatggt
       61 acgttaggct ttaacctcgg cgcatggcgc ttacgtagcg attaccaata taatcagaat
      121 tttgctgatg gacgctcggt aaaccgcgac agcgaatttg cgcgaactta tctgtttcgc
      181 cctatcccct cctggtcgtc aaaattcact atgggccagt acgacctgag ctccaatctt
      241 tacgatacct tccactttac tggcgcatcg ctggaaagtg atgaaagcat gctgccgcca
      301 gatttacagg gttatgcgcc acaaattacc ggcatcgcgc agaccaacgc gaaagtaact
      361 gtggcacaaa atggtcgtgt actttatcaa accactgtcg cgccaggccc ttttactatt
      421 tctgatttgg ggcaatcgtt tcaggggcag ctggatgtca cagtggaaga agaagatggc
      481 cgcaccagca ccttccaggt tggctccgca tccattccct atttaacccg taaagggcaa
      541 gtgcgctata aaacgtcact gggaaaaccg acatccgtcg ggcataacga tatcaataat
      601 ccctttttct ggacggcgga agcctcctgg ggctggctga acaatgtgtc gttgtatggt
      661 ggtggcatgt tcaccgctga tgattatcag gctatcacta ccggtattgg ctttaacctt
      721 aaccaattcg gttcgctttc ttttgatgtc accggagcag acgcgtcttt acagcaacaa
      781 aatagcggca atctgcgtgg ttacagctat cgcttcaact atgcaaagca tttcgaatcg
      841 acaggcagtc agattacctt cgcgggttat cgcttctcag ataaagatta cgtgtcgatg
      901 agtgagtacc tcagctcgcg taatggcgat gagtcaatcg ataatgaaaa agagagttat
      961 gtcatttcct tgaaccagta ctttgaaacg ctggaattaa actcttatct caacgttaca
     1021 cgcaatactt attgggacag cgccagcaat accaactact ccgtatctgt aagcaaaaac
     1081 tttgatattg gcgatttcaa aggtatatct gcatcgctgg cagtaagtcg aatccgctgg
     1141 gatgacgacg aagagaatca atattacttc tctttctctc tacctttaca acaaaaccgc
     1201 aacatctcct acagtatgca gcgaacggga agcagtaata cttcgcagat gatttcctgg
     1261 tacgattcat cagatcgcaa caatatctgg aatatttcag cgtcggcaac ggacgacaat
     1321 atacgtgatg gcgaaccaac actgcgcggc agctaccagc actattcgcc gtggggacgt
     1381 ctgaacatta atggcagtgt acagccgaat cagtacaatt ctgttaccgc aggctggtac
     1441 ggttcactta ccgctacacg tcatggtgtc gcccttcacg attatagcta tggcgataac
     1501 gcccgcatga tggtcgatac cgatggcatc tccggcattg aaatcaactc taaccgtacc
     1561 gttaccaacg ggctgggcat cgccgtgata ccttcgttat cgaactacac cacctccatg
     1621 ttgcgggtga acaataacga tctgccagaa ggtgtcgatg tcgaaaactc ggttattcgc
     1681 accacgctca cccagggtgc catcggctac gcaaaactga atgccaccac cggttaccaa
     1741 atcgtcggcg ttattcgtca ggaaaatggg cgcttccctc cactgggtgt gaatgtcacg
     1801 gataaagcga caggtaaaga tgtgggcctg gtcgcggaag acggcttcgt ttatctcagc
     1861 ggtattcagg aaaatagtac tctgcattta acctggggtg ataatacctg tgaagtcacg
     1921 ccgccaaacc aaagtaacat tagcgaaagc gcaataattt taccttgtaa aacagtcaaa
     1981 taattcagga tgaatataat gaaatcacca ttaatcacaa caggaatgat ttgtttactg
     2041 gggatctgca actttgccca ggcgactgtc tcccccgaca gaacacgcat catttttaat
     2101 gcttcgaata aaagcgcaac ggttcgcctg accaaccaaa gtaaaatcga tccctatctg
     2161 gctcagtcgt ggattgaaga tgccagcggt aaaaaaacgc gtgattacat ctcgacattg
     2221 ccgccgatgg aacgtattga gcctgatgaa cagatacaaa ttcgcctgat ggcactggcc
     2281 tcgttaaacg acttaccaca ggaccgagaa acgttgttct actataacgt gcgagaaatt
     2341 ccgccacgcg ccaaagaaca gaatgtaatg caaatcgcca tgcaaagccg cctgaagcta
     2401 ttctggcggc caaaagcgat tgagttaaaa gagggggaga tgattcctct gcaaaaagta
     2461 acgattaccc gcaccgccgc cggtctgacc ctgaataacc cgacgcctta tcatattacc
     2521 gtcggttata tcggcactaa tggcaagaca ctgatgcccg gtgcggacag tattatggtg
     2581 gcaccattta cttctgcaac tcaacaccta agcagcctgc cctctacctt ccagttaggc
     2641 tttgtggcgg actacggcgg gctggagatg tttaaagtcg aatgtaattc catccaatcc
     2701 ctctgccaaa gctctcctgc taagaagggg aaaatatgat tacgcttttt cgtttactgg
     2761 cgattctttg cctgtttttt aacgtttcag cttttgctgt tgattgctat caggatgggt
     2821 acagaggaac aaccctcata aatggagatt taccagcgtt caaaattcca gagaacgcgc
     2881 aacctgggca aaaaatttgg gagagtggag atattaatat cacggtttat tgtgacaatg
     2941 caccaggatg gacaacagac aatccatcag aaaatatcta tgcctggatc aaattacccc
     3001 aaataaataa tgccgacatg ttgaataatc catatttaac ctttggcgtg acttataatg
     3061 gtgtagatta tgaagggata aatgaaagaa ttgatactca tgcatgcctg gataaatatg
     3121 agcaatacta taatgggtat tattatgacc ctgtatgcaa tggcagtacc cttcaaaaaa
     3181 atgtaacatt taatgcccat tttcgcgtct atgtaaaatt caaaagccgc ccagcaggag
     3241 atcagacggt gaactttggc atagtcaacg tgctacaatt cgatggcgaa ggaggggcaa
     3301 acatgtcctc taacgcgaag aatttacgct atgtgattac gggattagat aatatttcat
     3361 tccttgactg tagtgtcgac gtccggattt ccccggaaag tcagatagtc aattttgggc
     3421 agatcgctgc gaattccatt gcaactttcc caccgaaggc agcattcagc gtttctacca
     3481 taaaagacat tgcgtctgat tgtaccgaac agtttgatgt tgcaaccagt ttctttactt
     3541 cagatacatt atatgacaat acgcatctgg aaataggtaa cggcttgctc atgcga
//
LOCUS       DAWDRC010000418         3596 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10417_length_3596_cov_2.124823, whole genome shotgun sequence.
ACCESSION   DAWDRC010000418 DAWDRC010000000
VERSION     DAWDRC010000418.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10417_length_3596_cov_2.124823"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    979..988
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1321..1330
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2208..2217
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1007 a          726 c          790 g         1043 t
ORIGIN      
        1 aggtattaca ggagagtttc acttctcgtc aggctgaaaa gcgctatttt ttatcctgga
       61 cccaaatgtg caactatttt tatgatatga acacgctagt tgatgccgga acagaaggtt
      121 taagactcat taaaacctgg cagcaagcaa gacctcattc tacacatgcc tggcttgccg
      181 aagcacaata ctggaatcac cgtgcatggt tatatcgcag ttatggctgg gcaaatgata
      241 ccacacatgc aatgtggctt tgtgctggtg catgtaatga acaaatggtc atcgctacac
      301 ttaaagcaat tgattgtgac ccacgacaat ggatggctgc attacttacc agcaccaact
      361 caaaagtatt tggtcagcca gcctggctgg cggcacacct taacggtgat agtgtcgcag
      421 gaataccatt aatgatcgca ctgaaaaatt atcaccgccg ctccccgcaa gaagtggaag
      481 cgttgatggc gtattccgga ctttcgtttg agcatgcaat atgccccgtt ttgcctcgcc
      541 ccaatatact tcctgagtat gatgatgatg gtgggcaaaa atattggctt tctgtttgcc
      601 tgactatttt tcctcacact ttttatcctt ttgttgaata tataccattt cgaatgccac
      661 gctggggagg gtcgcataaa gaaatttccg aattgctgga ttcagtaacc tgtaagcact
      721 tatctaccga agaacatgac tatatggatc tcttactttg gtgggatgat taccgagatg
      781 tcagcattga ggacattgcg cccgaggagc aacaatatgc aatcgactta gcagagaata
      841 ttgctcagca cgcacaattt caggaatgtc gccataatgc cctagagtgg ttacttgcct
      901 gctataacaa gcagaacgat cacgataagt tgtggtgctg tatacagcga gcggtgatgg
      961 aagacatgaa gctcaatann nnnnnnnntg cgtacgcgat aaaatttgca ttaagttatt
     1021 atccagattc attctggata tataatttta tctgccaaaa ttcacagaat acaacttatg
     1081 ctacacctgt tatttatagg gggtttttcc agcgagaggg tattttgggt tttgaaaaag
     1141 atgaagggca gggcgatgct tggctggaaa aagctagtga cattaaatac aatcataatt
     1201 ggcggtcggc aataaaagat ttgagttggt ttgacctttc tgattatttc attccgcttg
     1261 cgacaattgg taagcaaaga aatattcctg ccgcattaaa tttagttgct cttgaatatt
     1321 nnnnnnnnnn tttggataag gaagacaata caaaattacc ttatgaacca tctacagctt
     1381 tggagtattt tcgacgggca cttaaaatac ttcaagatga cctaaatttc catagctcgg
     1441 tatcttatcc actggtaaaa aattatggtt atagcgaaca tcaacaagat ctacaaaata
     1501 tttattttag cattgctatc tgctaccagg cactaaataa acaagaaatt tctaaagaaa
     1561 cacgggctat ttatgaaaaa aatctccttg acaatttatt tctggctcat gaagctggac
     1621 atgaaaaagc atgggggctt tttttactta atatctttga ggtgaaagaa ctctcacttg
     1681 ctcatttgca cctccaacag gtacaagaag aagcgaataa aggtacgctc gaagcaatga
     1741 ttactttgtc gcgcctgtat ggtaacaaag aagatgaaaa actgtttaac atgaaactca
     1801 gtgcgcgctg gacacatttt gccgagtctt tatatccaga taacgaaatt attgcagatt
     1861 gcctttatca tctccatttc tccagcctgt ggaaacgctg tcgttatgcc tggtatacct
     1921 ttcgtattcc agctagcgaa ttacccggcc aggttaacag tatggtgtaa ggtttatcct
     1981 gaaaattgtt aaatgtgtaa tatgagcccc atgatgagaa gatgcaaaca atgctgatgt
     2041 taaaaaaagg aatgatgctg gtggggctga tattcttttc tttacccgcc aatgccgggt
     2101 gtacgctgga ggaagccgcc agaaagatga ttatcggctt taagcaagaa gattgcccaa
     2161 ttaatcaatt cgttaattga taaaaaaacg ggattgtata ttattttnnn nnnnnnnttt
     2221 tccaccgtgg ttcttcgatg gatgttgaaa atagactggt gattgtgggg cgtgataaag
     2281 atttatccgc ccgcaaacag agatgctatt taaagggaat gtaagtccat agttcgctgt
     2341 gaaattcgag atcacaagta aaaatgtacc tgatgttggt gtgggtagac atttggttga
     2401 atagccgaaa ttaatgatga ttgtagcgct gacagtatcc actcctgctc aatcaacacg
     2461 aaggtaaggg ccgtcagcac taacattggc cccgaatccg cagttgtttg gcgtatcggt
     2521 gcttacatca agcgtttcgg tagtaagttt gagagtaata ttacagtgaa tcatatcgtc
     2581 tgtatcatca cgcaacacga ttacgcctgt gccattttta agcgttacag attcagaaaa
     2641 ctcaccaata tttggcccat tgtaaatgcc catcatacca aagtaattac cctgaaaatc
     2701 tacctgcatc gtgcctgtta accgcgcgag tttaaaggtg agcgtattcc atgtcgcttt
     2761 gcccgcgtag ttccagtatt cattggttgt tgaaaaatca gagggcaaag ccgcgagctt
     2821 atctttgata agttgcaaat tgtagatgga tttgggatct tgcggtagaa gcatgagcca
     2881 ggcacgggct ttgcgccatt gattctcgtg catccaggtt agcgcgacat tgttataagc
     2941 tgtggcgatc gctttctcat cattagcttt acagtcttca ctccatgcgg cttgctgttc
     3001 gaagtatttg cgtgccgcag ggtagttttt cgctttatac aatgcctgcc cctggcttgc
     3061 atacatagtg attttcgcgc agtcagcgtt aatttgctcg tcagttattt cagcatgtgc
     3121 attagccgac aatagcaaaa ctaaagatat gacaaagggt aattgcatgc taaatccatt
     3181 taaagtaaat aattccagtc gagatagtaa tgccgtggaa gtcaaaaata aagccggacg
     3241 ccaacaaccc acgtccggca tcgcaattac gtattgtctt tcaccgtctg gctggtgcca
     3301 ttggctttaa cggaggcaat tccggtttca cgagattgcg cagtggcgta catctggctg
     3361 gagccgataa tttgatgatt ggcggctttc aggttgaaat agaatttgcc gttgcttgcc
     3421 gtttttttct catagcgttc ttcttgcggg ctgttgctac gcaccgacgt gatgcccttt
     3481 tccgcagagg cttttgaggt atacagctcg ctggtgagga tagtctcacc attgcccgct
     3541 tttagcacaa accgaaattg attatcactg ctcttactga gttcaaacca accagc
//
LOCUS       DAWDRC010000419         3592 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10431_length_3592_cov_2.633588, whole genome shotgun sequence.
ACCESSION   DAWDRC010000419 DAWDRC010000000
VERSION     DAWDRC010000419.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10431_length_3592_cov_2.633588"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          880 a          963 c          937 g          812 t
ORIGIN      
        1 gaccattccg tcagttcgcc ctgaatatcg ccgggagcat gtagcttaag tccagcatga
       61 acagccgttt catcagccag gcgttcgacc acaggaataa actggataaa ctcaacgcct
      121 gcatcacaca gaaaatcata gatttgcaaa ggttgtaacg cactggtgcg atttacgcag
      181 accagcacgt tatagtcgac atgatgtttt tgcaggagcg tcagagcacg catcaccagc
      241 ttatgcgttg gtctgccacc tttggtcacg cgatattgat tgtggatctc agccggacca
      301 tccagcgata accctacaag aaaatgattt tctgccagaa atgcacacca ttcgtcatcg
      361 agcagcacgc cgttagtctg gaagctatta cttatcttcc tgccagcacc atatttcgcc
      421 tgtagcgcca cggcacggcg gtaaaaatcc agcccgagta acgttggttc gccgccctgc
      481 caggtaaaag ccacttcgtt ttgggtttcg ctggcagcgc tatagtgacg gacatacgct
      541 tccagcgtgt catcgtccat atgcgtgact ggcttttcgt ggtaaaggga ttgtttttcg
      601 agataaaaac agtagtcaca gttcagatta caatcagaac cgctcggttt cgccatcaca
      661 tgaaaagcac gcgttggaac ctgttgctgc atggtcgctc cttgcctgaa gtcatccttc
      721 aataagttaa gggcgaaatc ataccgtggc gcaatgcgtt attacgggcg tttattgcga
      781 agggaaagtg acgaaatgca cagaaaacaa aaaaccccgc cgaagcgggg ttcaaaattg
      841 gtcggcgaga gaggattcga acctccgacc cactggtccc aaaccagttg cgctaccaag
      901 ctgcgctact cgccgaaata ctgctttttg aatttttagt tcaattcttt aaagtcgtgg
      961 tgcgaggggg gggacttgaa cccccacgtc cgtaaggaca ctaacacctg aagctagcgc
     1021 gtctaccaat tccgccacct tcgcatacca tcaattctta aaaagaattg ctaccacgga
     1081 ggcgcattct agtggttttc agcttttcgt caatagttaa ttatcgacag aggtgtaatt
     1141 gctggaaaaa tgtccatcag cggattgtgg ataattcgtt tgtcggtcag ataaggcgtg
     1201 tacgccgcat ccgacaaggc atgcggcgag atacttaacc tttcttcgcc tggcgggtca
     1261 taatggcgcg atacaccttg aagcgcccgg tttgcgcaat cacttcgtgg aaaccaaatg
     1321 tctcatccag cacgtccggg taaggcagga aggcgttcgc tacaattcgc aactcgccgc
     1381 cgctgttcag atgacgcacc gcgccgcgaa tcagcgtttg cgcggcatcc aggctggttt
     1441 gcatcccatc gtggaacggc gggttggaga ttatcatatc aaagcgacct ttcacctcgg
     1501 aaaagacgtt actggcaaag acttcacctt caacaccgtt ggtcgcaagt gttgcgcggc
     1561 tggcttctac cgctggtgca gaaacatcac acagagtgag gcggatcttc ggtgaatggc
     1621 gcgcaaaagc aacggaaagc actcccgcgc cgcagccgac atccagcact ttacctttcg
     1681 tgtgtggagt tagcgtcgag agcagcaact gactgccgac atccagaccg tcgcggctaa
     1741 acacgcccgg cagtgttttg accgtcaagc catcgacgct gtattcgccc cagaatttat
     1801 tggcatcaaa taccggctgc ttttccagac gaccaaaata gaggccgcag cgacgggcgc
     1861 tgtctacctt attcaacggc gcataatcgg ccagcatctg ctcggcgctg cgcacgccgc
     1921 tgcggttctc gccaacgaca aaaatatccg tgcccaccgg aagcagagaa agtaaattca
     1981 tcaactggaa ctgggcttcc ggtttgttct tcggccagta gtagatcagc gtatcgcaat
     2041 ctgcgacgtc atctgccgtg gcgaccagac taaaacgggc gttatccccc atctggcggc
     2101 ttaacacctg ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac
     2161 gcgcgggcag gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat
     2221 cactgtgacg cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa
     2281 ttgatactgg cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt
     2341 gcgcccctga caacaggagc gattcgctat gacatcccga cgagactggc agttacagca
     2401 actgggcatt acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat
     2461 tgcgatcccg ccacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga
     2521 tcctctggtg agcgatgttc tgcgtgcgtt aactgtcagc cccgatcaag tgctgcaact
     2581 gacgccagaa aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg
     2641 tactgacgaa ccgctatcac tggaaggcgc tcaggtgaca tcaccggcgc tcaccgaatt
     2701 acgggcaaac ccaacggcac gcgccgcgtt atggcaacaa atttgcacat atgaacacga
     2761 tttcttccct cgaaacgact gatttaccag cggctttcca cattgaacaa cgcgcccaca
     2821 cctttccgtg gagtgaaaaa acctttgcca gtaatcaggg cgagcgctat ctcaactttc
     2881 agttaacgca aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag
     2941 ctacattgtt caatattgcg gtcgatcctg actatcagcg tcagggactg ggaagggtgc
     3001 tgctggaaca tctgatcgac gaactggaaa aacgcggcgt ggcgacgcta tggctggaag
     3061 tccgtgcctc aaatgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga
     3121 cgattcgccg caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt
     3181 taccaatcag tatgtaagac aaggtggaat aatgaagtgg gactggattt tatcaaaacg
     3241 gcgcgatcac ttcattacag cttcagcacg ggcggtttga gagctgggcc gaacggctga
     3301 acgtcgaacc gggtaaactc aacgaggcct ttattaatgc gatggcggaa atctgtacgc
     3361 cgttgccggg ggcggtttcc ctgcttaacg ccattcgtgg caacgccaaa atcggcatca
     3421 tcaccaacgg ctttagcgcc ttgcaacagg tacgtctgga acgcacgggc ctgcgtgatt
     3481 acttcgattt gctggtgatt tccgaagaag ttggcgttgc caaaccgaat aagaaaattt
     3541 tcgattatgc gctggaacaa gcgggcaatc ctgaccgttc acgcgtgctg at
//
LOCUS       DAWDRC010000420         3590 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10440_length_3590_cov_1.920226, whole genome shotgun sequence.
ACCESSION   DAWDRC010000420 DAWDRC010000000
VERSION     DAWDRC010000420.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10440_length_3590_cov_1.920226"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          850 a          924 c          905 g          911 t
ORIGIN      
        1 taatatcctg taataaagaa agattgccct gctggacaaa attaaacgct actttatcgc
       61 cggttttaat ttcgctcatt ttcgtctgcg gggtgatggt aaagcgcata gtcatctccg
      121 gccagttcac ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa
      181 caccttttac cacgccagtg gcgctaataa cctgtggttg tgcttcgctc atggtttcat
      241 gatgatgttc gttagcctgg gcattaaagc caataacggt aaacagacta aacattgcga
      301 cttgcagtgc ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc
      361 ccctaaggcg gtatataaag aaatttcgtt aacctgacgg gcataattca ggtcaagtaa
      421 agtttgtcgg gttgcaaata aagaacgctc ggcatccagc acttccagat aacttaccgc
      481 gccgtgctga tataatgccc gcgcccgttg caaagtaatt tgcagcgacg caagataacg
      541 ctgctgggcg ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc
      601 tttaaaggcg ttctggattt tctgttcata attcaccacc gactgctgct ggcgaatttc
      661 ggcgatatcc agattggcct ggttgcgtcc agcattaaaa atggggatct caattttagg
      721 aataaaattc cacatcccgc tgctggcatt aaataatgac gacagatcgc tgctggcggt
      781 tgatattccg ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt
      841 ggcattagcc gccattaacg cgtgttcagc ttccataata tcagggcgct gcaataagat
      901 ttgcgacgac aagcccgccg gtaatttaac gctttgcagg ctgtcgctgt ttactgtctg
      961 cgcttgcggc agcttgccgt agcttcccaa taacagttgc aatgcattat ttgcctgcgc
     1021 cagttccccc tgacgtttag cgatgtcgct gcgggtactt tctatcaccc cgcgagcctg
     1081 ttccagcgcc agaacattgc tgctaccggt caacagttgt ttttcgacaa acgcatatga
     1141 ctgctgataa ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagttgctg
     1201 attgaaatag ctttgcgcga cattagaaac cagcaaaata tgcaccgcgc gctgagcttc
     1261 ctcagtggct aaataatttt ggcgctcggc ttcgctcatg ttctttaagc gcccgaaaaa
     1321 atcgagatca aagctggcgt taaggccagt cgagaactcc cgcgtcgtgg ctgaatcgcc
     1381 tttaagattg ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc
     1441 atcggtcaga cgatattgcg cccgcgcttc ctgcactttc agcgccgcca tgcgcaaatc
     1501 ccggttattc accaacgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt
     1561 gcgccagccc gcgttctggt agttatctgc tgcgttaacc aggccgttct ggctgagtga
     1621 gaactgctgc ggcacgggca ttgccggacg ctgataatcc ggtgccagtg aacaaccggt
     1681 tagcgcaagg gcaacacaaa atggcagaag tttacaagga gacataggct cataatttct
     1741 ggtgatttta tgctgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg
     1801 acaaaattgt cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc
     1861 cgggcaacat gcggaggaaa tatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga
     1921 gaatacttga ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg
     1981 ctgaatggct accatctggc gatgaccggt gattatgatc tgataatcct cgatattatg
     2041 ctgccggacg tgaacggctg ggatatcgtg cgcatgttgc gttccgccaa taaggggatg
     2101 ccgattctgt tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg
     2161 ggggcggatg actatctggt gaagccgttc gcttttgctg aactgctggc gcgggtgcgc
     2221 actctactgc ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg
     2281 atggtcgatc tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt
     2341 aaagaattta ctctgctgga gtttttcctc cgccatcagg gcgaagtgct gccccgctcg
     2401 cttatcgcct cgcaggtatg ggacatgaat tttgacagcg ataccaacgc cattgatgtg
     2461 gcggtgaagc ggctgcgcgg caaaatcgac aacgactttg agccaaagtt gattcagacc
     2521 gtgcgcggcg tgggttacat gctagaggtg ccggatggtc agtaagccat ttcagcgccc
     2581 gttttcgctg gcaacccgcc tgaccttttt tatcagcctc gccaccatcg cggcgttttt
     2641 cgcctttgca tggatcatga tccactcggt aaaagtgcat tttgccgagc aggatattaa
     2701 tgatttaaag gagattagcg ccacccttga acgggtactt aatcaccctg acgaaacgca
     2761 agcccgacgc ttaatgacgc tggaagatat cgtcagtggt tattccaatg tgttgatctc
     2821 cctggcagat agccacggta aaacggtgta tcactccccc ggtgcgccgg atatccgcga
     2881 gtttacgcgt gacgccatac ccgataaaga cgcgcagggc ggcgaggtgt atctcctttc
     2941 cggcccgacg atgatgatgc cagggcacgg tcacgggcat atggaacaca gcaactggcg
     3001 gatgattaac ttgccggttg gcccgctggt tgacggcaaa ccgatttata cgctctacat
     3061 cgcgctttcg atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat
     3121 gaccgcatcg gtaatcagca tcctgatcgt ctttatcgtg ctgttggcgg tacataaagg
     3181 tcacgcgccg atccgcagcg tcagccgtca aatccagaat atcccctcga aagatctcga
     3241 cgttcgcctt gacccgcaga ccgtgcccat tgagctggaa cagctggtac tgtcgttcaa
     3301 ccatatgatc gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat
     3361 cgcccacgaa attcgcacac ctattactaa tctcattacg caaacggaaa tcgccctcag
     3421 ccagtcgcgc agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac
     3481 gcgaatggcg aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct
     3541 aatccccgaa aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt
//
LOCUS       DAWDRC010000421         3582 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10463_length_3582_cov_1.677630, whole genome shotgun sequence.
ACCESSION   DAWDRC010000421 DAWDRC010000000
VERSION     DAWDRC010000421.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10463_length_3582_cov_1.677630"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3330..3339
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          895 a          805 c          906 g          966 t
ORIGIN      
        1 acgcgtacgt cttgctgcgg tggtgctgca aatcacacca caagccaatc ccgcaggcca
       61 attgctgctt cttgatgagc cgatgaacag tcttgatgtt gcgcaacaaa gtgccttaga
      121 caaaattctg agcgcgctgt gtcagcaagg actggcgatt gtgatgagca gtcacgatct
      181 caaccacaca ttgcgtcatg cgcatcgggc gtggttgcta aaaggtggaa aaatgctggc
      241 cagtggacgc agagaagagg tgctcacgcc ggcaaatctg gcacaggcct atgggatgaa
      301 ttttcgccgt ctggatatcg aaggtcacag aatgctgatt tcgaccatct gatttcatta
      361 gtatcttgaa aatcgggtaa ttacgacgct aaattaatgc aagaataaaa aacagaggaa
      421 ccgtacggaa tgcgtttctg ccttattttg atcacagcac tgtttctggc cgggtgtagc
      481 caccataaag caccgccgcc aaatgccaga ctttctgatt cgattaccgt tattgccggt
      541 ttgaacgacc agctacaaag ctggcatggc acgccgtatc gttatggtgg catgacgcgg
      601 cgcggtgtgg actgttcggg atttgtggtt gtgacgatgc gcgatcgttt cgatttgcag
      661 ctgccccgtg aaaccaaaca acaagcctct attggcacgc aaattgataa agacgagttg
      721 ctgccaggtg acttggtctt tttcaaaacg ggttccggac aaaatggttt gcatgtaggt
      781 atttacgata ccaacaacca atttatccac gcctctacca gcaagggagt gatgcgttcc
      841 tcacttgata atgtctattg gcagaaaaat ttctggcagg cgagacgaat ctagtatgtc
      901 gaaaaatgga cggcgatgat acatcgccgt ccatttactc ctgaatacag tagtattttg
      961 cagacaaaaa ttccgtaaat ggattcttcg tttataatca ggataatcta aaaaaaggac
     1021 ccctgaaaaa gtggttaaca tggaacaaat gaaagttact ccttctgtaa ttgttatgtc
     1081 ttatggcctc atccctttta gccggatact gaaaaacatc cttcgagagg gacggttacc
     1141 atgaagattt ttttggagaa tctttatcat tctgattgct atttcctgcc gataagagat
     1201 aaccagcaag accttgtcgg cgttgagtta attacccatt tctccagtga agatggtacg
     1261 gttcgtatcc cgacgagtcg ggttatagcg caacttaccg aggagcaaca ttggcaatta
     1321 ttttctgaac agttggaatt actaaaatca tgccaacact tttttattca acataaactc
     1381 tttgcctggc ttaatttaac gccacaagtg gctacattat tattagacag ggataatttc
     1441 gcaggcgaac tattaaaata tccgtttatt gaactgttga ttaatgagaa ttacccgcat
     1501 ttcaatgaag gaaaagataa tcgcgatctt ttatccttat cgcaaatgta tccccttgtg
     1561 ttaggtaatt tgggtgcagg taatagcaca atgaaagcgg tttttgatgg tcttttcacc
     1621 cgcgtcatgc tggataaaag ttttattcag caacaaatca cgcatcgttc cttcgaaccg
     1681 tttattcgtg cgatccaggc gcaaatttcc ccttgttgca attgcatcat cgcggggggc
     1741 attgatacgc cagaaatatt ggcacagatt atcccctttg atttccatgc tctccaggga
     1801 tgcctgtggc ctgcggttcc tataaatcag ataacgacgt tggttcagcg ataaccctcc
     1861 tgtttgctgg tgtttaggac gagagtaacc gtctacacta tcaaacagga ggatctatga
     1921 ccctgtcttt tattacccgc tggcgcgatg aattgccaga aacctataca gcactttccc
     1981 ctacgtcttt aaataatgcc cggctgattt ggcataatac cgaactggct aacacgctga
     2041 gtattccatc gtcgctgttt aaaaatggcg caggtgtctg gggcggcgaa aacctactgc
     2101 ctggtatgtc accactggcg caggtttaca gtggtcatca gtttggcgtc tgggcgggcc
     2161 aactgggtga tgggcgcggc attttactcg gcgaacaact gcttgctgat ggcactacaa
     2221 tggactggca tctgaaaggt gctggcctga cgccttattc gcgaatgggt gatggacggg
     2281 cggttttacg ttcgacgata cgagaaagtc tcgccagtga ggcgatgcat tatctgggca
     2341 ttccgacgac ccgcgcttta agtatcgtca ccagcgattc gccagtgtat cgggaaacgg
     2401 tggagtcagg cgcgatgctg atgcgtgtgg caccaagtca tctgcgcttt ggtcatttcg
     2461 aacattttta ctatcgccgc gagccggaaa aggttcgtca gttagctgac tttgccattc
     2521 gtcattactg gtcacatctt gatgatgagg aggacaaata ccgtctctgg tttaccgatg
     2581 ttgtcgcacg taccgcctcg ttaattgccc aatggcagac ggtcggcttt gctcatggag
     2641 tgatgaatac cgacaacatg tcgctgctgg ggctgacgct tgattacggg ccgtttggtt
     2701 ttcttgatga ttacgaaccc ggttttattt gtaatcactc ggatcatcaa gggcgttaca
     2761 gctttgataa tcaacctgcc gtcgcgttgt ggaatttaca gcgtctggcg cagacattgt
     2821 caccatttgt tgccgttgat gccctgaatg aggccctgga cagctatcag caggttttgt
     2881 tgacgcatta tggacaacgg atgcggcaga aactgggctt catgacggag caaaaagagg
     2941 ataacgcgct actgaatgaa ttattcagtc tgatggcgcg agagcgcagc gattataccc
     3001 gcacattccg catgctgagt ctgaccgagc agcacagcgc ggcgtcaccg ctacgtgatg
     3061 agtttattga tcgtgcggca tttgatgact ggtttgcccg ttatcgggta cgtttgcaac
     3121 aagacgaggt taccgatagt gagcgtcagc agctgatgca aagcgttaac ccggctctgg
     3181 tgttgcgcaa ctggttggcg caacgggcga ttgaagcggc agaaaagggt gatatgacgg
     3241 aattgcaccg cctgcatgag gcgttgcgaa atcctttcag cgacagagat gatgactatg
     3301 tcagtcgtcc acccgactgg ggtaaacggn nnnnnnnnng ctggaagtca gctgctcgag
     3361 ttaaaggcta cttggtcaac agcagcttgc cagcctgagt tttacggagc agatattctt
     3421 gcccgtcatg atcgataatc agtttgccat ccggacctaa cagtgtctgg ctggtaattc
     3481 gccggatggg ctgcggggaa gcggtcttgt gattggcatc cgtttcaggc gtgagttttc
     3541 tgctatccgt ataacgcata aaaattaata ataatttata ta
//
LOCUS       DAWDRC010000422         3581 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10465_length_3581_cov_2.041690, whole genome shotgun sequence.
ACCESSION   DAWDRC010000422 DAWDRC010000000
VERSION     DAWDRC010000422.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10465_length_3581_cov_2.041690"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          756 a         1002 c         1025 g          798 t
ORIGIN      
        1 gggctggata acaacctgtt agatgccgcc agtaaaaccg gactatttgc caaaagcggc
       61 gtggcagcgg atgccgttaa cgttcccggc ggctggaata atgacacttt cgtcccgttt
      121 gattacggct atttcgcctt cgtctatgac aagaacaaac tgaaaaatcc gccgcaaagc
      181 ctgaaagagc ttgttgagag cgatcaaaac tggcgggtga tttatcagga tccgcgcacc
      241 agtacgccgg ggctgggcct gctgttgtgg atgcaaaaag tctatggcga tgacgcccca
      301 caagcctggc agaaactggc gaagaaaacg gtcacggtca ccaaaggctg gagcgaagcc
      361 tacggcctgt ttttaaaagg tgaaagcgat ctggtactga gttacaccac ctctccggct
      421 tatcacattc tcgaagagaa gaaagataac tacgctgccg cgaacttcag cgaaggtcac
      481 tatctgcagg tggaagtcgc cgcccgcacc gctgccagca agcagccgga gctggcgcaa
      541 aaatttctcc agtttatcgt ttctccggct tttcagaatg cgatcccaac cggcaactgg
      601 atgtatccgg tagcaaacgt cactctgcct gcaggatttg aacaattgac caaaccagcc
      661 accacgctag agttcacacc cgccgaagtg gcggcacaac gtcaggcatg gattagcgaa
      721 tggcaacgcg ccgtcagccg ttaattcccg gctggttaat tccaggcgta agcgccgcca
      781 cgctggtggt agcggttgca ctggcggcgt ttctcgccct gtggtggaac gcgccgcagg
      841 gtgactgggt ggcagtctgg caggacagct acctgtggca tgtggtgcgc ttctccttct
      901 ggcaggcgtt tctctcggcg ctgctctctg tcgtacccgc aatattcctc gcccgcgccc
      961 tctatcgcag gcgctttcct gggcgacaga tgctgttgcg tctgtgcgca atgaccttga
     1021 tcctcccggt gctggttgcc gttttcggca ttcttagcgt ctatggtcgc cagggctggc
     1081 tggcctcgct ctggcaatca cttggtctgg agtggacctt ttcgccctac ggcctgcaag
     1141 gcattttgct ggcccatgtg ttttttaatc tgccgatggc tagccgttta ttactccagg
     1201 cactggaaaa cattcccggc gaacaacgtc agcttgccgc ccaacttggg atgcgcggct
     1261 ggcatttttt ccgtttcgtc gaatggccgt ggttacggcg acaaatcccg ccggttgccg
     1321 cactaatctt tatgctctgt ttcgccagct tcgccaccgt gctatcgctg ggcggcggtc
     1381 cgcaggcgac cactatcgag ctggcaattt atcaggcgct gagttacgac tacgatcctg
     1441 cccgcgcggc aatgctggcg ctaatccaga tggtgtgctg cctcgggctg gtgctgttga
     1501 gtcagcgatt gagtaaggcc attgcgcctg gtaccacact gctgcaaggc tggcgcgacc
     1561 cggacgatcg tctgcatagc cgcatttgcg acacggcgtt aattgtgctg gcgctgctgc
     1621 tgttgctgcc accgttactg gcggtgatcg tcgatggggt aaatcgccag ttgccggaag
     1681 tgctggcaca accggtgctg tggcaggcgc tgtggacctc gttgcgtatt gcgctggcgg
     1741 caggcgtatt gtgcgtagtg ctgaccatga tgctgctatg gagcagtcgc gaacttcggg
     1801 cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag cgggatgttg atcctcgcca
     1861 tgccggggat tgtgctggct acgggcttct ttttactgct caacaacact atcggcctgc
     1921 cacaatctgc tgacggcatt gtgattttca ccaatgcgtt aatggcaatc ccttatgcgc
     1981 tgaaggtact ggaaaacccg atgcgcgata tcaccgcccg ctacagcatg ttgtgtcagt
     2041 cgctgggcat tgaaggctgg tcacgcttaa aagtggtgga gctgcgcgcc ctgaaacgtc
     2101 cactggcgca ggcgctggcc tttgcttgtg tgctgtcgat tggtgatttt ggcgtggtgg
     2161 cattgttcgg taacgatgat ttccgtaccc tgccgtttta tctctaccag caaattggct
     2221 cctatcgcag ccaggacggc gcggtcaccg cgttaattct gctgctgctc tgttttctgc
     2281 tgtttaccgt gattgaaaaa ataccggggc gaaatgttaa aactgactga tatcacctgg
     2341 ctttaccacc atttgccgat gcgttttagc ttaacggtgg aacgcggcga gcaattggcg
     2401 atcctcgggc caagcggcgc gggtaaaagc accctgctaa atttgatcgc cggttttctg
     2461 acgccagcca gcggtttgct gactatcgac ggcgtagatc acaccacaac gccgccgtca
     2521 cgccgtccgg tgtcgatgct gtttcaggag aacaacctgt tcagccatct gacggtcgcg
     2581 caaaacatcg ggctggggct aaatccgggg ttgaaactaa atgcggcgca gcagaagaaa
     2641 atgcacgcga ttgcccacca gatggggatt gataatttaa tggcgcggtt acctggcgag
     2701 ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt gtctggtacg cgaacagccc
     2761 attttattgc tcgatgaacc gttttccgca ctcgatccgg cgctacgtca ggagatgctg
     2821 acgctggtga gttcgagctg ccaacagcaa aaaatgacgc tcttgatggt gtcgcacagt
     2881 gtggaagatg cggcgcggat cgccacgcgc tcggtggtgg tcgccgacgg gcgtatcgcc
     2941 tggcagggta agaccgatga gttgttgagc ggtaaggcga gtgcttcggc gctgttggga
     3001 attaaaggtt aatatgcact gtcggatgcg gcgtgaacgc cttatccgac ctacaggtag
     3061 tgcgttttgt aggcctgata agacgcggca agcgtcgcat caggcaaccc gcacaaggct
     3121 atcctctaaa ccccaaccac tttacgcaaa atatcgatat acaccggcat caacgggtgg
     3181 cgaattaaca ccaccagcgc caccacgccg atggcagaaa tcaacggcgt cagccacaac
     3241 aaacgaccgc gggacaaata atgactcaaa cggtcagtcg ctttaccgct gcgccataac
     3301 cgccagcaca gccagcctcc aacccacaaa aacaccgctg ttgccagcag caaccattta
     3361 aactcaccgc tctgcatccc ggcaggaata tcgatcgccg cacccgccag aatccccggc
     3421 aggaagtaaa acggtggcca cagcaagcag ccgataatat tcggcgtaat aaattttgcc
     3481 accgggagat ccagcattcc cgccaccatt ggcaccagcg gacgcgtcgg accgacaaaa
     3541 cgaccgacca gaatggtgaa catgctgtgc tgatgcagcg c
//
LOCUS       DAWDRC010000423         3573 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10491_length_3573_cov_2.015634, whole genome shotgun sequence.
ACCESSION   DAWDRC010000423 DAWDRC010000000
VERSION     DAWDRC010000423.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10491_length_3573_cov_2.015634"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    915..924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1531..1540
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          868 a          829 c          937 g          919 t
ORIGIN      
        1 tcttggtcag gaaactaacg gcgcgggcgg tctgtttaaa ggtctgcgta ccattccggt
       61 gatttttgac atcgtaaaag atgtcgaaga actatgtccg aatgcatggg tgattaactt
      121 cactaacccg gcgggaatgg tcactgaagc cgtttatcgt cataccggat ttaaacgctt
      181 tatcggcgtg tgtaatattc cgatcggcat gaagatgttt attcgcgatg ttctgatgct
      241 gaaagacagc gatgatttat ctatcgatct gttcggcctc aaccatatgg tgttcattaa
      301 ggatgtgctg gtaaatggca aatcacgctt tgccgaattg cttgatggtg tggcatccgg
      361 gcagttaaaa gcatctggcg ttaaaaatat tttcgatctg ccatttagcg aaggcttaat
      421 tcgttctctg aatctgttgc cgtgttctta tttgctttat tacttcaagc aaaaagagat
      481 gctggctatt gaaatgggcg aatactacaa aggcggcgca cgagcgcagg tcgtacagaa
      541 agtcgagaaa caactttttg agctatataa aaacccggag ttgaacgtta agccgaaaga
      601 actggaacag cgcggtgggg cttattattc tgatgctgcg tgcgaagtga tcaacgctat
      661 ctataacgac aagcaagcag aacattacgt taatatcccg catcatgggc atattgataa
      721 tattccggca gactgggcgg tagaaatgac ctgtacgctg gggcgcgatg gcgcgacgcc
      781 acatccgcgc attacgcatt tcgatgataa agtgatgggg ctgattcaca ccatcaaagg
      841 cttcgagatt gctgccagca acgccgcact tagtggagaa tttaacgatg tcttactggc
      901 gctaaacctt tgtcnnnnnn nnnncgcacg ctcgttgatg ctcagaaagc gcgcgcagtg
      961 ctggacgatc ttcaagacgc gaaccagggc tattggcccg aactgctatg aaactgattg
     1021 ttaatgccga tgattttggc ttaagcaaag gtcagaacta cggcattatc gaggcctgtc
     1081 gcaatgggat tgtcacgtcg acgacggcgc tggtgaatgg gcaggctatt gaccatgcgg
     1141 tgcagttgag ccgtgatgaa ccgagtctgg ccatagggat gcactttgtc cttactatgg
     1201 gcaagcctct gacagctatg ccggggttaa cccgcgatgg tgtgctggga aaatggatct
     1261 ggcagttggc agaagaaggt gctttaccgc tggaagaaat tactcaggag ctcgccagtc
     1321 agtatttgcg tttcattgag ctatttggac gcaaacctac gcatcttgat agccatcatc
     1381 atgtgcatat gttcccgcag attttcccga ttgtggcaaa gtttgcggct gaagagggga
     1441 ttgcgttgcg catcgaccgt cagccgttat ctaacgatgg tgatttaccg gctaatctgc
     1501 gcagttcgca gggattcagt agcgcgtttt nnnnnnnnnn gaagtgatgt gccacccggc
     1561 gtttatcgat aatacgattc gccagagtgc ttattgcttc cctcggttaa cggaactgga
     1621 tgtgctgact tcagcgtcgt tgaaatatgc gattgctgag cgtggttatc tgttgggtag
     1681 ttatcatgat gtgtaagtaa ggtggtaagg tcactcccca ggggcgtgac cttatccata
     1741 tttacgccgg gattttgtca atcttaggaa tgcgtgacca cacgcggtgt gctgtcatca
     1801 gcgttaacag ttcatccata aaactaccat cggcgctgtc agcttccgca atcccttctt
     1861 caccctggtc agcgaccttg attgttgctt taaacttgcg cgcgtctcct gccagcgcaa
     1921 tcggtttaag gtgtttgtag gcttccatca gatagtagtt ggcatcgccg ttgtcagcga
     1981 tatccgcaat attgccgcaa gggacaatga ctgcatcgac cgtcagcgaa ggcgcaccgg
     2041 caaaggtagc agctataggc aacaccgtac cgtcatccgc agtcacttcc cccattcgcg
     2101 agtagagcag tttggcatga acgcctttgg ccttcagcgc cttgagaatg gccagaaggt
     2161 ctgccgatct cacttcatca ttaagcaaaa ttgctaccac gcgacctttc acatcaccgt
     2221 caggaatggc gtacagactt aaggatggat cctttttcag accgttgacg ttcggaggtg
     2281 gggtgatatt cagctggtca tcagtcagtt cgataccgag atttttcgcc accgcctggg
     2341 ccagagtgag atcaatatgc gccagctggt caacaacgcg ctcacgaata tacggacgaa
     2401 ctactttgct taactcaaaa ctgaaaccat cgacaatatg gcgatgctcg aatggcgtct
     2461 gacttagcca gaacagacgc ggatgggaat aatattcgcc aaacgatgga ctgcgctcgc
     2521 gaactttatt gccttccacg cgttcctggt atgattcaaa accgccgcgt ttcgaccccg
     2581 gcggtgtttc gcgcggccag ttatcgttga tcgagttcgg ttcgtaattc gccgggttag
     2641 tgtcgatccc catacgatgc atgccgtcac gctggaaatt atggtaaggg caggtcgggc
     2701 ggttaatcgg aatttcatgg aaattcggcc caccaagacg actgatttgt gtatcggtat
     2761 aagagaacaa acgcccctgc aataacgggt cgttggtgaa atccagaccg gggacaatat
     2821 gccctggatg gaaagccacc tgttcgtttt cagcaaagaa gttatccgga ttacgattga
     2881 gcaccatttt gccgacacgc tgaacaggca ccaattcttc cgggataagt ttggttggat
     2941 cgagaagatc gaagtcgaac ttgaattcgt cttcttcagg aatcaactgg aagcccagtt
     3001 cgtattcagg aaaatcgcct gcttcaatcg cttcccacaa ctcgcggcgg tggaagtccg
     3061 ggtcacgtcc ggttagtttt tgtgcttcat cccaaacgag tgaggcttta cctgccagtg
     3121 gtttccagtg gaaacgtacg aacgttgcct tcccttcggc attaatcagg cggaaggtat
     3181 gaataccgaa gccttccata gtgcggtaac tgcgcgggat accgcgatcc gacattgccc
     3241 acatcacgtt gtgcagagtt tcaggttgca gagaaacata atcccagaaa gtatcgtggg
     3301 cgctttgccc ttgtggaatt gcccagtgcg gttctggttt taccgcatga acaaaatcgg
     3361 ggaatttatg cgcatcctgg ataaagaaga ttggcgtgtt attgccaacg aggtcaaaaa
     3421 taccctcttc agtatagaac ttggtggcaa agccacggat atcacgtacg gtatcggcag
     3481 agccagcgcc gccctgaacg gtagagaacc taacaaatac tggggtgatt ttgttcgtat
     3541 ctgagaggaa atccgctttg gtgatatcgc tta
//
LOCUS       DAWDRC010000424         3551 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10552_length_3551_cov_2.693936, whole genome shotgun sequence.
ACCESSION   DAWDRC010000424 DAWDRC010000000
VERSION     DAWDRC010000424.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10552_length_3551_cov_2.693936"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          915 a          869 c          915 g          852 t
ORIGIN      
        1 aagacaaacc gagctacggt atggcgaccg atatgcaagg taatgtagtt ctgctggcgc
       61 tggatgaagt gaagcaaggt tcaatgccgg aagatcagaa aaaagcgatg gtgcaaggta
      121 tcactcagaa caacgcacaa atcgtctttg aagctctgat gagtaacctg cgtaaagagg
      181 cgaaaatcaa aattggcgat gcgctggaac agcaataatc ctgaagccgc ctcgcaaaaa
      241 aatgctttgc agctgtaaca agaaaaggtc gctttcgcgg ccttttccat ttctgaacat
      301 tgccatttgt ttactgtttt cactgccgtt aaggtgattc cactgttaac aaacaaggag
      361 aaaacagtat gaaacacgga attaaagcac tgctcattac cctgtccctg gcctgtgccg
      421 gaatgtctca tagcgcgctg gcggcagctt ctgtggcgaa accgacaaca gtagaaacca
      481 aagcggaagc tcctgcagca caaagtaaag cagcagtacc ggcgaaagcc agtgacgaag
      541 aaggcacccg ggtcagcatt aataatgcca gcgcggaaga gttagcccgc gcgatgaatg
      601 gcgttggcct gaagaaagcg caggcgattg tcagttatcg cgaagagtac ggtccgttta
      661 aaacggtgga ggatctaaaa caggtgccgg ggatgggcaa ttcgctggtg gaacgtaatc
      721 tggcggtatt aaccctgtaa ttaatttgca tagtggcaat tttttgccag actgaagagg
      781 tcataccagt tatgacctct gtacttataa cagttcttat aacaacaacg taaggttatt
      841 gcgctatgca aacacaaatc aaagttcgtg gatatcatct cgacgtttac cagcacgtaa
      901 acaacgcccg ctaccttgaa tttctcgaag aagcccgctg gcatgggttg gaaaatagcg
      961 acagttttca ctggatgacg gcccataaca tcgccttcgt cgtggtcaat atcaatatta
     1021 actatcgccg cccggcggta ttaagcgacc tgttaaccat taccagtcag ttgcagcaat
     1081 taaacggtaa aagtggcatc ttaagccagg tcattacact ggagccggaa ggacaggtgg
     1141 tagcggatgc gcttattacg tttgtttgta ttgatcttaa aacgcagaaa gcattagctc
     1201 tggaagggga attgcgcgaa aagctggagc agatggttaa gtaaacgttt tgtggtgccg
     1261 gatgctcaag ccgcatccgg cgacacccgg aataattact tcaacccggt tttctgcttc
     1321 atcgctgcca tcaccgtcgg tttatcggcc agataatgat tcaaaccgtt ggcacgtaaa
     1381 ttacacgccg cacaatgacc gcaaccgtcg cctttaatgc cgttataaca ggtcagcgtt
     1441 tcgttacgga ctaaatccag cttgccgtaa taatctgcca gcgcccaggt ttccgcttta
     1501 tcaatccaca tcagcggcgt ttcaaaacga atatctttcg ccatgcccaa actgacggca
     1561 tggtttagtg ctttcacaaa ctcatcgcgg caatccgggt agccggagaa atccgtttcg
     1621 cagacgccgg taattacggc ttctgctttt acctgatacg catatattgc cgccagcgtc
     1681 aggaacaaaa tattacgccc tgggacaaac gtattcggga taccatcggc ttcaggttca
     1741 taatcaggca ccggaatgct gtcacgcgtc aggctgctga ccgccagctc gttgagcagc
     1801 gttacatcaa gcaccttatg cgcgcgtgcc cccagtttca gcgccagttc gcgtgccacg
     1861 tcgatttctg cgcgatggcg ttgaccgtaa tcgaacgtca cgcaatggac ttcatcatat
     1921 tgttgtaatg cctgcaccag acaggtggtg gaatcctggc ctccactgaa cacaacgaca
     1981 gcacgtttca tagataatcc tgcttgaaca ataaaagcgt tatggtaacg cctgcgatta
     2041 acccggacca gctattcatt gcgacggcgc gggaagccag gcttcggtaa attcaaacca
     2101 gccgcgcggt gtcagtcgca caccgttcac gccgggaggg gcgctaatgc gatagtgata
     2161 gttgaacagc ggtgtcagcg tcgcatctgc cattaactgg ctaaaaaccg ctttcagggc
     2221 attaaatcgg ttttcctcat caggcattac ttgcaccgcg tccagtgtcg attgcaaatg
     2281 tgcatatgct ggagcgtcga aaacatgtgg ccacagtgga tcgcaacgca gccattgctc
     2341 cagggtatat tccggtgctt cgccaattaa tctgtcgccc atcatgaggt ctgcgtgtgc
     2401 cagtagggtc gtgtcgtccc agttttttgc gttatgaaaa ataattgtga gttcacagcc
     2461 ttccgctgcc agtgtcgcct gtaggcgttc tgccatggta tgaagttcta tcggtaggtg
     2521 ataaaccagc gtcaaggttt tcggtagttt aacttcatcc ggtacttgcc aatgcggaat
     2581 agtccagcct ggcagtaatg catgactggc agtgatcagg ttttctccga cttctaacgt
     2641 ttgtaataaa ccggattgat gaataatgga gatcactttt cgcgcctgcc agagggacag
     2701 tcggagactt ttgcgcaatg ttaaatagca aaaacctaaa ctgatgccgc tactgacctg
     2761 gctgacccgt tgcagctcct ccggtttgcc aatggtgatt tgcacgggat gccgacaact
     2821 ggttcccata tctttttcga aaagcggcgg agttatccag tactcaaccg ctttaagcag
     2881 cggatgacgt aaatggtaat aatcatggct ctccaggcgc accagttccg ctgtgaattg
     2941 tgttaagcga aaaggacctg tgccgatcag tgggaattgc ggatgcgcca gatggctgca
     3001 atagctcgcc agtcggtgcg caagccagta atcggggcga tgtaaaaaga aggtcagaca
     3061 ctgcggatgg gtgacttcaa tacgcttcac gctaataaat aattgatcca gtgctggcag
     3121 ttgtaacagc atcaataatc gctggtgtaa gtgtgaggct tttactgcat cgccgttatg
     3181 ccagtgtagc gttgaacgaa gataaaagtc ccagcgtaac ccgtcagtag aggtttccca
     3241 gtgatgtgct aaatcgccaa tcgggcgctg agtattatta tcgaagcggg tcaggccgga
     3301 aaatatctgc ccggccagat gctgctcggc acggccgggc aaaaagcccg gttgtagcgg
     3361 ttcgagcggg cgatagtagg gaatacgcaa cgtgggcgta tcgttttgcc attgtccgcc
     3421 cataaacggc tgtaacagag tgcgcagctc acctggggcc agttgcgcca gctccagcac
     3481 atcttgcggc tttccggttt ccagcgcctg ttccatcatc gcattgcgta gcgattctgg
     3541 cgtgaccaga a
//
LOCUS       DAWDRC010000425         3546 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10567_length_3546_cov_2.570324, whole genome shotgun sequence.
ACCESSION   DAWDRC010000425 DAWDRC010000000
VERSION     DAWDRC010000425.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10567_length_3546_cov_2.570324"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          838 a          849 c          911 g          948 t
ORIGIN      
        1 ctggcgcgcc gcaagatggg aataagaacc tttggggccg agaaaagcga tgcgtgctga
       61 gtgcggatta attttattga gatgttgctg gagcaaagcc tgctgagtta atacggaatc
      121 ttcaatgatg agctggaaca ggcgagtaat gtaatgggcg tccagatggt gcgctttacc
      181 gagcgtaatt aatctttcca gcaaatcgcg ttcacgatca atatcacgta ccgggcgatg
      241 cgagagcagt ttggcttttc ccacctcgac ggccagttcg cgtcgctctg ccagtaatgc
      301 taataatttt tcatccagcg cgctgatttt ctctcgcagc gccagtaacg ggttttccga
      361 tgtcatagtg ttgccttttt gttatcaata aaaaaggccc cccgatttgg gaggccttat
      421 tgttcgtctt cgcattctgt ttcacacgac gaaacgcctc ccattcaggg gaaggtaaaa
      481 aagaatgcga agaaaaacgg tgtgtgtttc atgtttgttt ccttaagtga ctttagtaca
      541 gtacccgtac tgttttcacg ctgtcaacaa aaaacgcgcc cgaaggcgcg ttggcgatac
      601 actcaatata aaggactact cttcttcaac ttcttcgacg aagttggcgt ctttcaccga
      661 tgttgcggca cgacgtgctt cgcctttgtg ctgcagttta ttgagctgcc gttccagctt
      721 gttgatcaat tcgttaattg cggtgtacat atcttcatgt ttaccactgg caaccagaac
      781 gccgttaggt gtattgattg tggcgtcagc aacaaaccct tgtggctctt tggacagaat
      841 gatatgtgga ttaatcagat gtgtttgcca tttttccagt ttggcgagac ggtctgcgac
      901 atgttggcgg atggccggag taatttccat ttgtttgctg gtaatgttca ttgtcataaa
      961 ttttacctct tgtcttcccg tcttggtgaa ttcagcataa cgttcctgat gtcaaaatgt
     1021 gtgatggaaa tctcataatt ttgtcacttt ttgtcaacga atcttttttg tgagaaatcg
     1081 caggaagagg tgttttttta cttgaggaat atctcaaagc gggccatatt tgatgagatc
     1141 gatagtgact aaatcgcttc agtttcacaa ctaacagaat aaaaaaacgg cagctcaagg
     1201 ctgccgtttt gtgtttcagg tttctgttat gtattgctgc tgtttgcggc gatgattttc
     1261 gctacttttt cagcttgcgc attcatctgc atctgacggt atgcattttc catcagcggc
     1321 agcgcatcac gcgtagcctg ggtatccgga tagtcgcgca acatgccttc tacgcggtta
     1381 acgacagcaa cccatgcacc acgttctgta taatactcgg ccacggagta ttcatatttc
     1441 gccagacgat ctttcaggaa taccagacgt ttggtggcat cggtggtgta ctgactgttc
     1501 ggatagccgc gcaccagttt ggaaaagtca ctaaacgcag ctcgtgcatg ttgaggatcg
     1561 cgatcgctac ggtcaacgcc aaagaaccct tgcagcgcac tgtcatccag cgccatattg
     1621 gtcaggccac gcatgtacat gacataatcg atattcggat gggtcgggtt aaggcgaata
     1681 aaacgatcga tggcagcctg tgctaacggc aaatcggcgt ttttatagta ggcgtagatg
     1741 agatccagct gcacctgctg cgaatacgga ccaaacggat agcgattatc taacgcttcc
     1801 agttgcgtta ttgcctgtct ccagttaccg tcctgaagcc tttgttgtgc agtcgcgtaa
     1861 atttcatttg gcggattatc aggtacttct tcctttgacc ccgagcaacc cgccaaaaac
     1921 aggcttagtg tggcggctgc caccagatat ttcatgcgcg tcatgacgtt ttgactttcc
     1981 tcaaaatgta atacgggaga ttctctgttc ctgctcccgg ttaagaccag ctacaatagc
     2041 acactatatt aaacggcaaa gccgtaaaac cccaacgata aacgaagaag cagtatatat
     2101 ggcacaacga gtacagctca ctgcaacggt gtccgaaaac caactcggtc aacgcttaga
     2161 tcaggctttg gccgaaatgt tcccggatta ttcacgttcg cgaataaaag aatggatcct
     2221 cgaccagcgc gtgctggtta acggcaaagt gtgtgataag ccgaaagaaa aagtattggg
     2281 tggcgagcag gtggccatca acgctgagat tgaagaagaa gcgcgttttg aaccgcagga
     2341 tatcccgctg gatatcgtct atgaagatga agatatcatc gtcattaata aaccacgcga
     2401 cctggtggta catcctggcg cgggtaaccc ggatggtacg gtactgaatg cgttgcttca
     2461 ttactatccg cccattgccg atgtaccgcg tgcgggcatc gtccatcgtc tggataaaga
     2521 caccaccggc ctgatggttg tggcaaaaac cgttccggct cagacgcgtt tagtcgaatc
     2581 tttgcaacgg cgtgaaatta ctcgtgagta tgaagcggtg gcaattggtc atatgaccgc
     2641 tggcggtaca gtagatgaac caatcagccg ccacccgacc aaacgtactc acatggcggt
     2701 gcatccgatg ggtaaaccgg cggtcactca ctatcgcatc atggaacact tccgtgtgca
     2761 cacgcgtctg cggttgcgtc tggaaactgg acgtacgcac cagatccgcg tgcatatggc
     2821 ccatatcact catccgctgg tgggcgatcc ggtttatggt ggccgtccgc gtccgccaaa
     2881 aggtgcttcg gaagcgttta tctccacgct gcgtaagttt gaccgccagg cgcttcatgc
     2941 aaccatgctg cgtctttatc acccgatctc cggtatcgaa atggaatggc atgcgcctat
     3001 tccacaagat atggtggagc tgattgagga gatgcgcgcc gatttcgaag agcataagga
     3061 tgaagtggac tggttatgag taagctgatt gtcccgcagt ggccgctgcc aaaaggtgtt
     3121 gcggcctgta gctctacccg tatcggcggc gtgagcttgc cgccttacga ctctctcaac
     3181 cttggcgctc attgtggtga taacccggat cacgttgaag agaatcgcaa gcgacttttt
     3241 gctgcgggca atttgccttc aaaaccggtc tggcttgagc aggtacacgg caaagatgtg
     3301 cttaagctta ctggcgaacc ttatgcctca aaacgagctg atgcctctta tagcaatacg
     3361 cccggcacgg tttgcgcagt gatgactgct gactgcctcc ctgtgctgtt ttgcaatcgt
     3421 acgggaacgg aagttgccgc cgctcatgca ggctggcgcg ggctgtgttc tggcgtgctg
     3481 gaagagacgg tttcttgttt tgctgataat ccggaaaata ttctcgcctg gttagggccg
     3541 gcaatt
//
LOCUS       DAWDRC010000426         3543 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10582_length_3543_cov_2.031250, whole genome shotgun sequence.
ACCESSION   DAWDRC010000426 DAWDRC010000000
VERSION     DAWDRC010000426.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_3543_cov_2.031250"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    334..343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1054..1063
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2870..2879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          905 a          863 c          905 g          840 t
ORIGIN      
        1 gcgtaaacct gaactggagg tgaaccatgg cgcagtttga atgggttcac gccgcctggc
       61 tggcattggc aatcgtgctg gaaatcgttg ctaacgtctt tttgaaattt tctgacggct
      121 ttcgttgcaa aatatttggc ttgctctccc tggcggcggt gctggctgcc tttagtgcgc
      181 tttctcaggc cgttaaaggg atcgacttgt ctgtcgctta tgcattgtgg ggcgggtttg
      241 gtattgccgc cacgttagcc gcaggttgga tcttgtttgg tcaaaggtta aatcgtaaag
      301 gctggattgg cctggtgctg ctgttggctg gaannnnnnn nnnaacttgc ctgatgaaca
      361 cgctgcccgc gctgtcgggc agcgtttgaa cattattttt gcgacagttg atccagcttg
      421 tcgcgaaaac cggtaacaga aatggcccgg ttatcggcgc gccagcgatc tttcgcggca
      481 ggagccgagc tttgcacccc aattaattgc cagccgtcat cggtatgcaa catcagaggc
      541 gaaccgctgt cacccggcaa ggtatcgcac tgatgcgaca tcaccgacgt ttgcgcccag
      601 ccagtcactt cacagttttg atgactgtac aacgtatcga gatgatcttc agggtagccc
      661 gcctgagtca ctttacgacc agccgctttt aatgcggcgg taagcgcggc tttatctccc
      721 tcaaataacg gcaacggtgt aatgccagaa gggggattac gtagcacaat caaaccgaag
      781 tcccagggcg cagctgcggg aggtacaatc cagccatccc catctgcttt taaccgcttt
      841 cccagtgtcg gatcaacgcg gccttctatg tcgtggatct catagcgcca aagaccttta
      901 tttgacacga aacgcagcgc taccgcttta tcggctttac cctttggcgg cgtcaataaa
      961 cagtgtcctg ccgttaatgc cagattgggt gcaatcagtg tcgccgtaca taaattgccg
     1021 ctggccgttt ccagttggcc aaccgcatcc cacnnnnnnn nnnccccacg gtgattgggt
     1081 cgtgtcattc actggcacac gatcatcatg accaaaaaac agggtgctga cctcatcgtt
     1141 tgccgatttg gcaacgtctg gtttatctgc aaacacaaaa gcagacgtca aactaattgc
     1201 acccaacact acagcaatgg ttgtacgcat atcacactct ggtgggtaat tatgattatt
     1261 aaaagcaaac cctcataaat actatagacg ggacgggatg aaagtgggag taaaatcaga
     1321 taactacgat caggaaagat aaaaacgggc ggcaatcagt gcgcataaaa taaggatgat
     1381 aagaatgaac tcaaagcgat aacggcccac catactaccc tccgctaaag gcggtgctaa
     1441 acgttacccg cttagcaccg taattttcag gcgcgagggg gcgcgccagc attactgttg
     1501 aaaacttacg ctgcgggttg tgcagcaggt tttgccggtt gctgatggct gtgtttcttg
     1561 gcgtgtttct tcgccgcctg cgctttttgt tcaggggctt tctgttcagc tttggcattt
     1621 ttatgatgct ttttagccgc ctgcgctttc tgggcaggtg ctgctttatg ctgtttttta
     1681 tgatgtgtgg ttttcgcagg cgctgctttg gtggtcgtcg cagtcggagc aggtgtggtc
     1741 gcagtctctg cagcaaaggc agcagaagac agacccatag cagcggcaac aaccagagct
     1801 aatacttttt tcattgtcat accctcaatt tgttttttca tttaacccca ctgcggggcc
     1861 gttgaaataa ctatatccct gggaatggca gacttccgtg agtggttggt ttcagcgtgt
     1921 aacgatatgt acaaacgctg aataaattac agcgttgata ataacgtatt gtgacttaag
     1981 gaaaatttag ctaccaataa tagtagtctt gatcgtgtta actattttac tacttacaaa
     2041 gcggtagaat aactgcgcat cagtaataaa tgacacacag caaaatgaat ccgtttattt
     2101 gggtacttat aatcctgatg acgctagatg cgctgcggga attggctggc gcttcgtcta
     2161 ttttaggatg gctattaacg ttggtttgag caggcaataa gtccggacgg gtatttacct
     2221 cagtccggac ttattttttc aggcgtgcag acgatgatgc aaacgcgtcc cgaccagcag
     2281 agcaatgacc agcatcagag caataaatgc gccgacgccg ttccagccat agttatgcca
     2341 gaaaacacca cccagcgtcc cggcaatact cgaccccaga tagtaactga acagatacag
     2401 cgaggaggcc tggcctttag cgcgttttgc gcgggggccg atccagctgc tggcaacgga
     2461 gtgggctgcg aagaatccgg ctgagaagag taacattccg gcaaagatca gccacagcga
     2521 gctgaataag gtcatcagta aaccaaacag cataaccccc gtcgaaaaca acagcaccgg
     2581 accgcgtcca tagcgggtgg tcatggttcc ggctttgggt gagctccatg taccggtcaa
     2641 ataagccagc gataataagc caaccacggc ctgactgaca tgccaggggg aaagcatcaa
     2701 cctatagccg atgtaattaa acagcgtgac gaacgacccc attagcaaaa agccttctgc
     2761 gaacaataac ggtaatcccc ggtcacgcca gtgcagacga aagttgataa ataacgtctt
     2821 agggcgcagc gaagtcgggc gaaaatggcg tgattcaggg aggattttcn nnnnnnnnnt
     2881 caacgccgag gccaacgcga aacaaccgat tgccgccaga gcaattcgcc agttaaaaaa
     2941 gtccgtgaag acaccgctaa ttaagcgtcc gctcatgccg ccaattgagt tgccgctgat
     3001 atacaacccc attgaaaagg ccacgaaact ggggtggatt tcctcgctaa gataagtcat
     3061 gccaacggct gccacgccac ttaacgaaag cccaatcaag gcgcgcataa tcaaaatgcc
     3121 gtgccagctg gtcatcattg tcgaaagtaa cgtacaaatg gaggccaaca gtagcgccgt
     3181 gaccatcact ggtttgcgac caatggcatc ggatagcggg ccagtaaaca gcaaaccgat
     3241 agccaacatc gccgttgaaa tggacagtga aatactactg ttcgcgggcg ttaagccaaa
     3301 ctcttgtgaa agcaccggaa ggataggttg cacacaatag agaagtgcga aagttgccag
     3361 tccggcagag aaaagcgcca gggtgacgcg cataaattgc ggcgtaccgc gtttaataaa
     3421 ttgatttggc tgagaaatgc tttgcttgtc agtgtcgctt gccggagcgc catcaacagt
     3481 tgtagtacgg ctcacttgaa atccttgcta aatatgcctg tggatcaggc ttatgaatag
     3541 att
//
LOCUS       DAWDRC010000427         3538 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10601_length_3538_cov_2.509905, whole genome shotgun sequence.
ACCESSION   DAWDRC010000427 DAWDRC010000000
VERSION     DAWDRC010000427.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10601_length_3538_cov_2.509905"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          834 a          989 c          844 g          871 t
ORIGIN      
        1 caggggcagc tggttcagtt cgcttcggcg tctgtgcgac tggcttcggt tccgttttca
       61 cggttgcttg cggttttttc ggttcaatca ccgcctgctg acgagctggg cgcgtagtgg
      121 ccggacgttc agcggtttgc gttttcgccg tgtcacgcga tgcattgcca ttgcgaacag
      181 gcgcaaccgt tgcgggttca gtcggcaatg tagaattgac cgccacattg ttcaactgtt
      241 gctgattttg tggctgggtc agcgcattgt tcaggtcacc ctgcacttca acacgttgtt
      301 gaccatccgt tgccaccggg gtttgccctt gagtcggcgt agaagagatc ggcggcagag
      361 aaacatcctg ctgagtattt tccgcagacg tggttcccgg cgctggctgc acgccattgg
      421 cctgatcggt cgcattgcct gcaagatcaa tactcttctc gccagacgcg gtttgatcgc
      481 tggaagaggt cgagggggct tttagcgcag aaccgatacc gatgatcaac agcagtagaa
      541 ccagaatgcc gacacccatc atcatatact gacgagaagc gggtttactg gctgcttttt
      601 tgcgcttacg cggacgacgc tctacgcgct cttcatccac ggtttcatct tcggattcgt
      661 caatttcttc ttcgatttcc ggttcctcat tacgcgcttt ttgcgcacga gtcggacggc
      721 gatcgtcagt gtcatcaagt tcaatatcat caaaattgat ctgcggttcg ccacgttcag
      781 tacgctcaga acgttcagaa gattgacgag aacgaccagt acgacgatcg ctgggatcgg
      841 gtttcagctc gtcttctggt ttgaattcat ccatttaaca ccccactaaa aggttaatgc
      901 ttaccacgtt gcaattaacc tgaagctaat agaccgcttg ataagcggcc tgacctttct
      961 tgttgttacg ctgattgaca atcggcaatg gcgttaagaa caagctcgtg cgaaacgccg
     1021 ctgcgaactt cactcttacc aattgccaac ggaagaatta agcgcatctc tcccgcaagg
     1081 actttcttgt cacgcagcat atgcggtaaa tacgcctgcg cggacatttc gcgcggccca
     1141 ttgaccggta acccagcccg cgtgagcagg gttataatac gctgcgtttc ggcagaacta
     1201 aactgcccga gacgttccga cgtccgcgcc gccatcacca tacccgcagc gaccgcttca
     1261 ccatgtaacc aattgccata ccccatttca gcttcaatgg catgaccaaa ggtgtgtccc
     1321 agattcagta aagcacgtaa cccggtttcg cgctcgtcgg cggcgacaac ttctgctttc
     1381 agctcacaac aacggcgaat acagtacgcc attgccggac cgtccagacg caacaacgca
     1441 tccagattct cttccagcca gttaaaaaac gcaccgtcaa gaataatgcc gtatttgatg
     1501 acttctgcca gccccgacgc taactcacgc gggggaagcg ttttcagaca gtcgagatcc
     1561 accaccactg aagcgggctg gtagaacgcg ccaatcatgt ttttaccgag gggatggttg
     1621 accgcagttt tgccgccaac ggaggaatcg acctgcgaca gtaacgtcgt cgggacttga
     1681 atgaaacgta caccgcgctg ataactcgcc gccgcgaagc cggtcagatc acccactacg
     1741 ccgccgccaa gcgccaccag agtagtatcg cgaccatgcg gcttttgtaa caatgccgta
     1801 aagacggtat cgagtacagc caggctttta tactgctcgc cgtcagggag gataacgcta
     1861 tcgacgttaa cacccgcctg ttcaagtacg ccgcggacct tatcgagata cagaggagcc
     1921 agggtttcgt tggtgaccaa catgacctgc tcgcccgatt tcagcggtaa gaatgaagct
     1981 ggttcattaa acaaaccaga tgcgatggta attgggtaac tacgttcccc gagagtaacg
     2041 acaatcctct ccataacgcg acatccacct taattactgt acccgcagac gagtgtatat
     2101 aaagccagaa ttagttgctt tccagcatgt gaataatctg gtttgcaacc actttagcgc
     2161 tttgatcatc agtacgaatg gtcacgtcgg caatctcttc atacagcgga ttgcgttcat
     2221 tggccaacgc ttccagaact tcacgcggcg gtgtttcaac gtgcagcaac gggcgttttt
     2281 tatcacgctg cgtgcgtgca agttgctttt cgatggtcgt ttcaagataa acgacaacgc
     2341 cacgagcgga aagacggtta cgcgtttcac gggatttcac agagccgccg ccagtagcca
     2401 gcacaatacc ctgtttctcg gtcaactcat tgatgacctt ttcttcgcga tcgcggaagc
     2461 cttcttcgcc ttctaaatcg aaaacccagc ccacatcagc tccggttcgt ttctcaatct
     2521 cttgatcgga atcgtaaaat tccatattga gttgttgagc taactggcgc ccaatagtgc
     2581 tttttccggc acccataggc ccaaccagaa agatattgcg tttctctgcc attttttcgg
     2641 tactactaag actattcgtt aatgataaac ccgcttcgct cagagagcgc cgcaggacat
     2701 gaactgaaac ctcataagat attgcgagag tcagactgaa aattatctca atactccagc
     2761 gggtttggca actgaataaa tcacccatcc ctctgcatat ctggtcgctg caagcgcgct
     2821 gccttgctac caccgctctg gcgataaatc accgggtaag attagcgtaa aaaagacagc
     2881 aaaatgccgc ctgaatgata aatcatcatc atggaacgca ggcagcgaag agcaccaact
     2941 ctcaaatcgg tactccttgt atgctaaata cctgcgcgcg tcaaatagat gaaacacgtt
     3001 cagcgtaaaa acattaccgc ttttacggct gtttactcac tggaaaccag tcgtggcgtg
     3061 ataaacacca ctaactcgcg tcgttcatct tcttttccgt catgacgaaa taattgcccg
     3121 aaccagggaa tgtcgccaag caacggtacg ctatcctgac ccgatttatt tttacgggta
     3181 aaaatgccgc ccagcgccaa cgtttctccg cttttgacct cgacctgcgt ttcgatctcc
     3241 tgcttatcaa tcgccagcac ttcgccatct gcctgctgta gcacctgccc cggaacgttc
     3301 tggctgatgt gtaatttcag ccggatgcga cctttttgta acaccgtggg cgtgacttcc
     3361 atccccagga cggcctcttt aaattccacc gacgtcgcgc cactttcccc gctggaaacc
     3421 tgatatggaa tttcgctccc ctgtttaatg ctggcaggct gaagatgtga ggccagcaga
     3481 cgcggactgg cgataatatc cagctgctgt ttttgttcga gcgcggaaag ctcaagat
//
LOCUS       DAWDRC010000428         3536 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10605_length_3536_cov_3.676242, whole genome shotgun sequence.
ACCESSION   DAWDRC010000428 DAWDRC010000000
VERSION     DAWDRC010000428.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10605_length_3536_cov_3.676242"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          873 a          914 c          827 g          922 t
ORIGIN      
        1 ttgccctctg acttccattt atcctgcact ttcttcacag caaggttacg acgttcaata
       61 gatgcctgat ccgcaaccca ggtgccttcg atatcgaagt ccaggactct gaggttcagg
      121 ttatcaacga tgtcataata atgctgcatt agatcgtcaa cgttcttaca ggaagctgcc
      181 agcggggcat tattagcacc accgatagac agcatcacat caccgccagc ctcacgcaga
      241 gctttgattt tgctgtactg agcgtaattc tgcataccgt aagcggtacc ccatgtcggc
      301 agacaggtgt tcgcatcttt actcaccaca aacgccagcg tgaagtggtt gacgttatga
      361 ttcttagcca gcgcagccaa atccgggatc gtattcagcg taaagtcgac atacggtgca
      421 taaacgtgtt tcggccacgc tttcttcggc gtacctacgc gctctttggt tccggtccag
      481 tcaacaaaaa cacgccacgg gttgctgtca gaaggagaaa ctttctgcac tttactctga
      541 gaaatgtagt tctcaccgtt aaagcgaatc agcgtatcac tggcataaag cgtttctgga
      601 ttaaactgcg gcgcattatt cagctcttcg ttgctatagc tctgagtctt acctaacggc
      661 ttccacgggc gactattgct accggtggcg ttttggttcg caaccagagc agggttgtca
      721 ctttgcgtcc agaacagcgc ttcataagcc tgcccatcaa caacaacgcg ctcacctttc
      781 acgtacacgg tggaagcgga ccatgctttc gccggttcgt atttcttcca cgggttatcg
      841 ccgccgggga caaagcccca tgcgtcttga tcgacagagg ttttataatg tgcatcgtta
      901 taatctacga tgctgttcgc gctgtacgct ttgctggcct ggaaaccatc cgcaataatg
      961 acgccgccat tatcaatttc acaggtagtc gggttaccgt actgactcat ttccgcagcg
     1021 gtagctgtac gcacgaggcg ccatgggtta ttggagtttt ctacactggt gccacgcggg
     1081 caatcttcag aaccaaccca ccaggcattc tgataaacat taccattgaa gataacatgg
     1141 taaatttcgc tgccttcctg gccactccac gcctgcagtg agtaatcgtt actgctatcc
     1201 gctggtgttg atggctcgtt gttatccgga gttggagtaa ctggagtttc cggtgtttcc
     1261 ggagttggat caaccggagt cacagcaccg ccattattat ccggcttaac ggaacaggag
     1321 cctgggttac catactgagt gatttctgtt gccgttgccg cgcgaacata acgccatggg
     1381 ttgctggcat cgttttcttt cgcatcgcct gggcaatttg ccgcgcctac ccaccaggcg
     1441 ttcttataaa tgctgccgtt gtagataacg taccatgtag aagaaccttc ctgacctttc
     1501 caggccacga cagcatagtt gtcgtttgac ttatcacctg gggctggctg aacatcagga
     1561 gccggtttaa cgtccggtgc cggtttcaca tccggtgcag gctgtggtgc tgaagtacaa
     1621 gactgtggat tactggtttc tgagatttcc gttgccgtag cggcacgaac ataacgccat
     1681 gggttgctgg catcattgcc tttcgcatca cccgggcagt tagaagaggc tacccaccag
     1741 gcatttttat atatcgcacc attaaagacg acataccagg tttgaccgcc ctgctgttta
     1801 ttccaggcaa ctacagaaga attagacgga acagtgccct gtgctggtgt agccgaaccg
     1861 ccattagcag gcgtattaga tgctggcgta tttgaagttg aagaagatga gcttccgctc
     1921 ttttcgcagg aaagtgtatt gccaaactga ctaatttcag cagctgttgc ggtacgcttt
     1981 aaacgccacg ggttagttgc atcatttgct ttcgcttttc ccgggcaatt tgtagaagaa
     2041 acccaccagg cattttcata aattttgcca tcgaaaataa cctgatattt attaccacct
     2101 tgttggttat tccatgcttc cattgccaat gctggcagag cggaacacac cagccccata
     2161 ccaatcatag atttagtaaa tatatttaat ttcattataa tcccttgtaa cgtaaaaact
     2221 gcaaaacaaa agtcctacgc aggaattatt tacgttttac gagaaccctt caatatgaat
     2281 taaagtgcgg catgaaaaat aagaaaaaag cctgacaaat gaagcatttt aaaaacagaa
     2341 acattcatat ttaaaatatt acattgaatt gatattttaa atatgaataa tttattcgtt
     2401 ctgacagtac gaataagata tgcagtcaac aaatgcaaaa agggcgccga agcgcccttt
     2461 tcaattcaaa actaattaac gtgtaattag cccagaactt tagcaacaac gcccgcgcca
     2521 acggtacggc cgccttcacg gattgcgaaa cgcagaccgt cgtccatcgc gatcgggtgg
     2581 atcagggtaa caaccatttt gatgttgtcg cccggcatta ccatctctac accttccggc
     2641 agttcgatgg taccagtcac gtcagtagta cggaagtaga actgcggacg gtagcctttg
     2701 aagaacggag tatgacgacc gccttcatct ttggacagaa tgtacacttc agattcgaac
     2761 ttggtgtgcg gcttgatggt gcccggctta gccagtacct gaccacgttc gatttcttca
     2821 cgtttgatac cacgcagcag aacacctacg ttctcaccag cacggccttc gtccagcagt
     2881 ttgcggaaca tttcaacgcc agtacaggta gacttctgag tctctttgat accaacgatt
     2941 tcaacttctt caccaacttt gatgataccg cgttctacac gaccggtaac aacggtacca
     3001 cgaccggaga tggagaatac gtcttcgatc ggcagcagga acggcttgtc aatcgcacgc
     3061 tctggttccg gaatgtaaga atccaggaag ccagccagtt ccaggatttt cgcttcccac
     3121 tctgcgtcgc cttccagcgc tttcagagca gaaccacgaa cgatcggagt gtcgtcgccc
     3181 gggaagtcgt actgagacag aagttcacga acttccattt caaccagttc cagcagctct
     3241 tcgtcatcaa ccatgtcgca tttgttcagg aacacgatga tgtacggaac gcctacctga
     3301 cgacccagca ggatgtgctc acgagtctgc ggcatcgggc cgtcagtcgc agcaactacc
     3361 aggatcgcgc cgtccatctg agcagcaccg gtgatcatgt ttttaacata gtcggcgtgc
     3421 cccgggcagt ctacgtgcgc gtagtgacgg gtcggggtgt cgtattcaac gtgagaagtg
     3481 ttgatggtga taccacgagc tttttcttcc ggcgcgttat cgatctggtc gaatgc
//
LOCUS       DAWDRC010000429         3524 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10643_length_3524_cov_2.092822, whole genome shotgun sequence.
ACCESSION   DAWDRC010000429 DAWDRC010000000
VERSION     DAWDRC010000429.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10643_length_3524_cov_2.092822"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1938..1947
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2665..2674
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          949 a          849 c          909 g          797 t
ORIGIN      
        1 tccgttaatg tgaatcattc ttttatgtta tgattttaaa aggaatttta tgaaaagcct
       61 ctcctataag cggatctata aatcacaaga atacctggca acgttgggca caattgaata
      121 ccgatcattg tttggtagtt acagcctgac cgttgacgac acggtgtttg cgatggtttc
      181 tgatggtgag ttgtatcttc gggcttgtga gcaaagtgca cagtactgtg taaaacatcc
      241 gcctgtctgg ctgacatata aaaagtgtgg ccgatccgtt accctcaatt actatcgggt
      301 tgatgaaagt ctatggcgaa atcaactgaa gctggtgcgt ctgtcgaaat attctctcga
      361 tgcagcgctg aaagagaaaa gcacgcgcaa tacccgggaa agactgaaag atttgcccaa
      421 tatgtctttt catctggaag cgattctcgg ggaggtgggg attaaggatg tacgggcgtt
      481 acgtatactt ggggcaaaaa tgtgttggtt gcgactgcgg cagcaaaaca gtctggtgac
      541 agaaaagatt ctgtttatgc ttgaaggggc cattatcggc attcatgaag ctgcgctccc
      601 ggtggcacgc cgccaggagc ttgcagaatg ggctgactct cttacgccga aacaggagtt
      661 tcctgcggaa cttgagtaat ctggcgttgc agacgaccaa tctctggcag taatgcaatc
      721 aataacccaa cttgttgtac gaccagaggt tctttgctgt ctgcccgtgg ttcaagttgc
      781 tgcatccgct gtttcaggcc agctaatgcc tgattgacgc gttcttcatc agcaggttga
      841 tgatgtagcg cgtcatcaac atagcaaacg gcgtcatcaa gaaacgccag aatttcagga
      901 ttagttaact gctcccggtg agcaccgagg gctgagatat agctggtaaa cgtatggtta
      961 aggcacagca accgaaacgc ggcttcgcga atttgcgggg taacgttcgg ttcgctggac
     1021 atatttgata ccaccgacgc cagttcagca tcacggttgt gtgcatcgcg gcgggcaata
     1081 cgatacgcca gacggttatc acgcccctga tggtattgtt ccagtatggc atcgagatac
     1141 cggcagttgg cctctgtggc gcgttcgagc attcgcggca gattgcgaaa cttccagtca
     1201 ggccagatgt agctcactgc cgcccacgca atggcacaac caatcagcgt atcgattacg
     1261 cgaggtaacg ccacttcaaa accttcaccc agcaagttga aacacagtag caccaaaagt
     1321 gtgatgaaca tcgttgcatg ggcgtattgc acgttacgga aggcaaaaaa gagcacgccg
     1381 gtaataacca gcagcaccag ctgcccttcc agtgatggca caaaccacag cacaggaata
     1441 ccaattgcga tacctaccag cgtaccaata atccttaact tcaggcggtg gcgcgtggcg
     1501 ttatagtttg gctggcagac aaacaaactt gtcagcaaga tccaataccc gtgatgcatt
     1561 ccggttatct gaatgatggc gtagccgaag cacaacacca gcgacattct taccgcatga
     1621 cggaagaggg cggattccgg cgtgaagtga cggctaagac gcagccagat atcactcaat
     1681 ccgtgcgggc tgtcatcagc gagctcattt tcgtcattat tatggggcag tgcctgggcc
     1741 tgttctgatt caattgttgc cagttgggca tcaatggcac gtaaattgtt cagtaaaaat
     1801 cccagtgttt tgagtaaatc agcaggagcg ccgttatcgc gcatccgctc cagcgcagca
     1861 tcaatatgcg taaaagcgcg ctcaaaatgc ggatcatgtt gataaggctg acgcagcaaa
     1921 atacagcgtg acagttgnnn nnnnnnngaa tatgagaaga gctggcacgc tcgtgaatat
     1981 cctgtgcgac aaagtaataa tgcagtgtgc gacgcgttcc ccgttgacca cgatcgccac
     2041 gtaagcgggt cagcagcgag agtttcgtct gattcaatgt cgccatcagc tgaccgttgg
     2101 cgagagccaa atcgtacagc ggtgcctggc tttcatcttc aatatcagga tcaaacatgc
     2161 gcgacttgag ctcaagataa cgcgccagtt gttcatagca acgcgccagg ttgtcctgca
     2221 gcgggcggac cgggaacagc agatgaccaa taagtgtcag gacgttgtac cagacggcac
     2281 cggccagaag atacatcggc tgctgatacc agtgctcata cagtgatgtt cccaacatag
     2341 tgtaaatggc gatcagcaat gcaccgaagg caattgttgc atagcgttga cccagaccgc
     2401 cgagcaaaat gaagccgctg gtagagagcg ttaagccaat cgcaaatagc cagggccagg
     2461 gaaacagcaa ttctactgag gccgaggcga taaaaaagca gaacagcgta atgatgaggt
     2521 tacgcaaacg tcccgccagt cggtcatcga gatcggtcag cgccgctgcc accatcccaa
     2581 gcgtcagcgg aatcgtcagt tttacatcac ccagccacca cggaaacgct gtggttccac
     2641 aaagcgcaat aaaaatacgc gcgtnnnnnn nnnngcgcgc gtaatacagc caggcgctgt
     2701 tccaggtata gcgtttgagc aaaggactta gcataaaggc catattagtg gattattcaa
     2761 aacgacgacg tgcattggct tcgcgcgcag cttgcgctgt ttctaccgat accacgcgac
     2821 gaccgaccgg ccatagtgca atagcggcaa ttttaaagtt cgcaataccg acaggaatgc
     2881 caatgatgga aatacattgt gcgatgcccg ttgcaatgtg catcaggcat aaccaccagc
     2941 caaagaaaat cagccagaaa atattcaata ccgtaccgcc agtattcagc agcacatttt
     3001 tgccagccgg gttcagttca tcgacatgaa tagcttcatt gccataaggc accagagaca
     3061 gtttagtgat ctcccagcag gatcgtgtca gcggtaaggt aaaaatcagc acaatactga
     3121 ccagagtcgc caacagccag cccagagtgg tggcaaatcc gccaagcaca aagttcagaa
     3181 tgttcaaaac ggtacgcata aaacctcgct ttactgtggt tttcagtaat gggcggcaat
     3241 tgtaacgttt ttttgggctg gagcacgttt tctctgacgg ttacactgat aagaaataat
     3301 ttcgtgtgga tctacagagt catggaactg aaagcgacaa cgcttggaaa acgtctggca
     3361 cagcaccctt acgatcgggc ggtgatcctc aatgccggga ttaaagtctc cggcgatcgc
     3421 cacgaatacc ttattccttt caatcaatta ctggcgattc actgtaagcg cggtctggta
     3481 tggggcgaac tggagtttgt actgccggac gaaaaagtgg tgcg
//
LOCUS       DAWDRC010000430         3518 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10663_length_3518_cov_2.929830, whole genome shotgun sequence.
ACCESSION   DAWDRC010000430 DAWDRC010000000
VERSION     DAWDRC010000430.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10663_length_3518_cov_2.929830"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          939 a          908 c          855 g          816 t
ORIGIN      
        1 ggccgaagaa ctcactgacc agtgacggga acaccgtaat cgtgccacca aagttaaatg
       61 ccacacaggc aatcgctgca aagaacgtca ctgcattcaa tggtgcaaac agcagggccg
      121 ccatacccac cagcgcaatc acctgaccaa tggtgataac acggatacgg gcgattttgt
      181 cagacagaat gcccagcacc agacgaccag aaaggttggc gatggaaata accgtcacag
      241 cattggctgc ggaaattgca tcaagatgcg ccaggctttg agcgatatct ttcgctacac
      301 caatcacata cagaccactc atgcacgcag tcaggaacat aaccgctaac atccagtact
      361 gcggtttacg catcgactct gccagggtgt agtccttctc caccacacca ttgctggttt
      421 tcacttcctg cttcggcgca tctttcatta acgttgcgcc aaagacaatc atcaccagta
      481 caatcgcgcc ccaaataaca aaggtttttt ccagacccac cgtttcgagc aggtgcgtgt
      541 cgataaattt aaaacccagg ctgcccagac cataagaacc gatagcaaaa gcagagataa
      601 gtcccttacg ctccggaaac cacttcacgc agttagagag cgtcaacagg taacccgcac
      661 catccgccag accaaccaac acaccggcgc ttaaccacag catcatcagg ttgttcgaat
      721 gtgctgtcag gaagaaacct aatcccagca aaatgccgga agccatagtg acgcgtttaa
      781 caccaaaacg ttcctgcaat ttgcccgcaa cagaagacga aattgccagc cccagactta
      841 acaagccgaa agagaaagca acctggctta caggagcccc cagcttggca gaaagcgcac
      901 cgttaaacag actccaggta taaaccgacc ccagagcaaa ctgggtaatg atagtaccga
      961 tgagtgtcag ccagcgagta cgctgataat ttgaaggtgt catggcagta ttcctgcagt
     1021 aataaaaagg aaaaatctct gctgacaacc ataacgaagt gcgctttatg cctggggaaa
     1081 aacggcatga aatgccatca gaacggaatg aaatgcatta gttcaggaat gaatgactcg
     1141 acaattattc gccaaattta ttatttagta agttagtccc cactatcgcc acattagttg
     1201 ttatttatgg gtttcaattg tatctataac gttaaaatct ggcgctaacc cccatcgtat
     1261 acagatagtc gctattgtta gtggtatttt ctgcctgaga tagcgcagca taggcagcaa
     1321 tattttgatt gagcaacata tcagcgccta cggtgacatc cagccagttg ccgttctggt
     1381 ttgtcgccgt catccggctc aggcctgatt gcgccttcca gatattctcg ccaaattgct
     1441 ggttatagct gatttgcgcc cagggtcgaa gatcgccaag ccgggagtca acacgccagc
     1501 ctaaggtact cacgctggca tgccataacg gatcggccag cgtggtagta ctgtcgccaa
     1561 actcgttata catcgaggta gttgtgccat cgtaatgtaa tgccgcgacc gggccggttg
     1621 taacctgctc atataaagga aaattccagc ccatactcat actgctggtc acatccacag
     1681 gggtttcttc cgccagattg acttccagtc ccagcgccct ttggctactt tgaatacgtt
     1741 gcgacaaaat atcgttgtaa tcaggttgat gatcgcgatc ccaggtgtaa cgctctgtgg
     1801 aatgtccggg ttgcgtatca acgatatatt cttgttgcca ggcgtaagct gtgttgagaa
     1861 aaaaggctct gattaccacg ctcgcaagca ggcttagctg ccagcgacca ccgctttttt
     1921 ttatgatcat taacacgact ccagggaaga gccgaattcg gcaatattat tgtcattgta
     1981 tgaaggatat cgggcatagt agccctgtat taaatattga ctttttcacc gatgcgtcaa
     2041 gaaaagcggc tgaaattttt acgatcgggt acatagcgag ggaaagtatg gaacgttgcg
     2101 gctgggtgag tcaggacccg ctttatattg cctaccatga taatgaatgg ggcgtgcctg
     2161 aaactgacag taaaaaactg ttcgaaatga tctgcctgga agggcagcag gctgggttat
     2221 cgtggatcac cgtcctcaaa aaacgcgaaa actatcgcgc atgctttcat cagttcgatc
     2281 cggtgaaagt tgccgcaatg caggaagagg atgtcgaaag actggtacag gacgccggga
     2341 ttatccgcca tcgtgggaaa attcaggcaa ttattggtaa tgcgcgggcg tacctgcaaa
     2401 tggaacagaa cggcgaaccg tttgccgact ttgtctggtc gtttgtcaat catcaaccac
     2461 aggtgacaca agccacaacg ttgagcgaaa tccccacatc tacacccgcc tccgacgccc
     2521 tatctaaagc actaaaaaaa cgtggtttta agtttgtcgg taccacaatc tgttactcct
     2581 ttatgcaggc atgtgggctg gtgaatgatc atgtggttgg ctgctgttgc catccgggaa
     2641 ataaaccatg attcgggaag tgcaacgttc agaactcccc gcgatcctcg aactgtggct
     2701 ggaaagtaca acctgggggc atccctttat aaaatcgagt tactggcgtg actgcattcc
     2761 gctggtgcgc gatgcgtatc ttgccaacgc gcaaaactgg gtctgggaag aagacggcaa
     2821 gcttctcggt tttgtcagta ttatggaagg ccgatttctg gcagcaatgt ttgtcgcacc
     2881 gaaggccgtc aggcgcggta ttggtaaggc gctgatgcag tatgtgcagc agcgccatcc
     2941 ccatctgatg ctggaggttt atcaaaaaaa tcaaccggcg atagatttct accatgcgca
     3001 gggttttcac attgtcgatt gcgcatggca ggatgaaacc caactaccca cctggattat
     3061 gagctggccg gtggttcaaa cgctgtaagt gtcagttccg gaccggtgta tttttccagc
     3121 catgccagcg ccgtattacc cgcacagcca ttccccagcc gcgagctggg gagatctttg
     3181 gtcagcacgt tcactgcgcc gtttttacaa atgccgccag cggttaaatc caaatccggc
     3241 catgcgcctt cgtgaataca aatcacgcca ggtttaattc cctcgctaat gaccgcgccg
     3301 gcaagaattt gcccgcgtga gttccacagc cgaacagtat cgccatcttg tatgccgcgc
     3361 gtctgggcat cgtcaggatg aatggtgaca ggctcacgat ttgccaccgc gtacaattcg
     3421 cgcagagaac tgtaattcag ctggctgtgc agacgatgcg ccggatgagc agaaagtacc
     3481 tgcaactgtt ccggttcggc actgccctgc cattcgtc
//
LOCUS       DAWDRC010000431         3507 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10708_length_3507_cov_2.563441, whole genome shotgun sequence.
ACCESSION   DAWDRC010000431 DAWDRC010000000
VERSION     DAWDRC010000431.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10708_length_3507_cov_2.563441"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    592..601
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2278..2287
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          864 a          872 c          926 g          825 t
ORIGIN      
        1 actacctata aactggtgcg taaagtcttc cagaataccg aacacgttca cgtcgtcggc
       61 ctgcgaggat ttgcgccgca acttcctgaa acgcttccag gaagtgacga agcgccttct
      121 caggcgagtt aaaattaagg cggcagcaat gccgcctttt ctttttccga aaactccgtt
      181 tctgcactaa aaaagtggca cattacggcg cgcttttctt taaacttctt tacattaggt
      241 tatgtaaaaa cgtatcggcg tttatatact gaagataagc ctgatgagta acaggcttgc
      301 tcgtcatact ttcgtgagta ttggcgttgt acaggcaagt cgtaaaataa cagcctggct
      361 attcagagta tgataaaaac agggggcaag ggatgttgct taatatgatg tgtggtcgtc
      421 ggctgtcggc aatcagtttg tgcctggccg taacattcgc tccactgttc aatgcgcagg
      481 ccgatgagcc tgaagtaatc cctggcgaca gcccggtggc tgtcagtgaa cagggcgagg
      541 cactgccgca ggcgcaagcc acggcaataa tggcggggat ccagccattg cnnnnnnnnn
      601 ngcggctgaa aaagcccgca cgcaaatcga atctcaatta cccgcaggtt ataagccggt
      661 ttatcttaac cagcttcaat tgttgtatgc cgcacgcgat atgcaaccca tgtgggaaaa
      721 ccgtgatgct gttaaagcct tccagcaaca gctggcagag gtggcgattg ccggtttcca
      781 gccgcagttt aataaatggg tagagttact gaccgatcct ggtgttaacg ggatgacacg
      841 cgacgtggtg ctctctgatg cgatgatggg ctatctccat ttcattgcaa atattccggt
      901 caaaggcact cgctggctat atagcagtaa accttatgcg cttgcaacgc cgccgctttc
      961 ggtgattaac caatggcagc tggcgctgga taaaggtcaa ttgcctacgt ttgttgcagg
     1021 actggcaccg cagcatccgc aatatgcggc gatgcatgaa tcgttactgg ccttactcag
     1081 tgacaccaaa ccgtggcccc aactgaccgg caaagcaacg ttgcgcccag gacagtggag
     1141 taacgacgta ccggcgttgc gcgaaatatt gcaacgtaca ggcatgttgg acgggggacc
     1201 gaaaattacg ctacctggcg atgacacgcc aactgacgcg gtagtcagcc catccgctgt
     1261 tactattgaa acagcagaaa ctaagccgat ggacaagcaa actacgtctc gtagtaaacc
     1321 tgcgcctgtc gttcgtgccg cctacgataa tgaactggtg gaagccgtta aacgttttca
     1381 ggcatggcaa ggattggggg cagatggtgc tattggcccg gcaacgcgtg actggttaaa
     1441 cgtaacgccc gcccagcgtg ctggcgtgtt ggcactcaac atccagcgat tgcgcttgct
     1501 gccaacagag ctttctaccg ggatcatggt taacattccg gcctattcgc tggtctacta
     1561 tcagaacggc aatcaggtgc tggattcgcg agtcattgtc ggtcgccccg atcgcaaaac
     1621 gccaatgatg agcagtgccc ttaacaacgt agtggtaaac ccgccgtgga acgtcccacc
     1681 aactctggca cgcaaagata ttctgccgaa agtgcgcaac gatccgggat atctcgaaag
     1741 ccatggctat acggtgatgc gcggctggaa caacagagaa gcgattgacc catggcaggt
     1801 tgactggtct acaatcacgg cctcgaattt accgttccgc ttccagcagg ctccaggccc
     1861 acggaactcg ctggggcgct ataaattcaa tatgccgagt tcagaggcca tttatttgca
     1921 tgacacgccg aaccacaatc tgttcaagcg tgatacacgc gcattgagct caggctgtgt
     1981 acgagtgaat aaagcttccg atctggcgaa tatgctgttg caggatgcag gctggaatga
     2041 caaacgtatt tctgatgcgc tgaagcaggg cgatacgcgt tacgtcaata ttcggcagtc
     2101 gattccggtg aatctctact atctgacggc ctttgttggt gcagatggtc gtacccagta
     2161 tcgtacagat atttacaatt atgatctgcc tgcgcgatcc agctcgcaaa tcgtatcgaa
     2221 agcggaacaa ttaatcaggt aaatgaagca gttcggggaa atcagttgtc gtaataannn
     2281 nnnnnnntag catgatcggg ggcgattctc tgcagccccc gtcactgctg gggttgagta
     2341 atcttgacgt ctgctttacg ggcggttaag gtgcctcttg tgcgccagaa gtgcatataa
     2401 acgataacat tgacctgtag acttgattat catggacaaa ttcgacgcta atcgccgcaa
     2461 actgctggcg cttggtggcg ttgcactcgg tgccgccatc ctgccgaccc cggcgtttgc
     2521 aacactctct accccacgcc cgcgcatttt gacactcaat aatcttcata ccggagagtc
     2581 aatcaaagcg gagtttttcg atggcagagg ctatattcag gaagaattgg caaaacttaa
     2641 ccattttttc cgcgattacc gcgcgaacaa aataaagtcc atcgacccag gattattcga
     2701 ccagttgtat cgcctgcaag ggttgttagg cacccgcaaa ccggtgcaac tcatttccgg
     2761 ttatcgttcc attgatacca acaatgaact acgcgcccgc agccgtggag tagcgaagaa
     2821 aagctatcac actaaaggcc aggcgatgga tttccacatt gaaggtatcg cgttaagcaa
     2881 tattcgcaaa gccgcgttat ctatgcgcgc aggtggtgta ggatattacc cacgtagtaa
     2941 ctttgtgcat attgataccg ggccagcacg gcactggtag taatcgctta acgaaacagg
     3001 ggcagtatga actatcgtat tattccggtc accgcattct cccagaactg ttcattgatc
     3061 tggtgtgaac aaacccgtct ggccgcactg gtcgatcctg gcggcgatgc ggaaaaaatc
     3121 aaacaggaag ttgatgccag cggcctgaca ctgatgcaga tcctgctgac tcatggtcat
     3181 ctggaccacg ttggcgcagc ggcggaactg gcgcaacatt acggcgtgcc ggtttttggc
     3241 ccggaaaaag aagatgagtt ctggctgcaa ggcttgcctg cgcaaagtcg tatgtttggt
     3301 ctggaagagt gccagccgct gacgccagat cgttggctga acgaaggcga taccatcagc
     3361 atagggaatg tgactttaca ggtgttacat tgccctgggc atacgccggg tcatgtcgtg
     3421 ttttttgatg atcgggcaaa gctgctgatt tctggcgacg ttattttcaa aggtggagta
     3481 gggcgcagtg acttcccgcg tggcgat
//
LOCUS       DAWDRC010000432         3505 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10721_length_3505_cov_2.142899, whole genome shotgun sequence.
ACCESSION   DAWDRC010000432 DAWDRC010000000
VERSION     DAWDRC010000432.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10721_length_3505_cov_2.142899"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1775..1784
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          870 a          831 c          880 g          914 t
ORIGIN      
        1 accacgcgca agatccgttg gtgacatata ccttcggctt taccggacgt tcagaactgg
       61 atactgcctt taaccgcgca gggttaacgc cgcgtatcgt cttcacggca acggatgctg
      121 acgtcattaa aacttacgtc cggttagggc tgggggtagg ggtcattgcc agcatggcgg
      181 tggatccggt cgccgatccc gaccttgtgc gtgttgatgc tcacgacatc ttcagccaca
      241 gtacaaccaa aattggtttt cgccgtagta cttttttgcg tagttatatg tatgatttca
      301 ttcagcgttt tgcaccgcat ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct
      361 ctaatgaaga aattgaggtc atgtttaaag atataaaact gccggaaaaa taatttcttg
      421 cgttattttc ggcacctttt atgtagcgaa ggtgccggaa tatattctcc tttgttactt
      481 atttgtatcc ggtgcacaat atttttatcc ctcatattta tagggtaaat tacctgtaaa
      541 gtagtgtcta attacacacc tcgcaaactc cagttttcaa atatctattt caggtcaaaa
      601 gattgaatta ttcactggag acgattcgta aattcgctgg atttttgact aaagtttctt
      661 taggatttgt ctcatcgtat gattaattac accaatcgtt caacgtcttg ctgataagtg
      721 atggcgatta tatgaggtta gcaatgcctt ctggaaatca ggaaccccgc cgcaatcctg
      781 aattaaaacg taaagcctgg ctggcggttt ttcttggttc tgcacttttc tgggtggttg
      841 tcgcactgct tatttggaaa ttgtggggat aactatggtc ggtcaggagc aactggagtc
      901 gtcaccatta tgtcagcata gtgacaatga accagaagca aaacgggaat gttccgtcgt
      961 tattccagac gactggcaac taacatcgca gcaacaagcc tttatagaac tgtttgctga
     1021 agatgatcag ccgaaacaat aattatcatc attcttatta cccattttta atgaattaca
     1081 gggcttttaa tacaccgcag caataacagg ttgagttatc tcaacacaaa gtaataaccg
     1141 ttaagggtgt agcctatgat caacacaaat atgaaatatt ggtcctggat gggcgcattt
     1201 tctctgtcga tgctcttctg ggccgaactc ctctggatca ttactcactg atccctgacc
     1261 ccgctgtttg ctttgcctca aggtttctcc tcttttatca atttgggttg ttatcaaatc
     1321 gttacgcgat gtttgtgtta tctttaatat tcaccctgaa gagaatcagg gcttcgcaac
     1381 cctgtcatta aggaggagct atgtcgtcaa ccctacgaga agccagtaaa gacacgttgc
     1441 aggccaaaga taaaacttac cactactaca gcctgccgct tgctgccaaa tcactgggcg
     1501 atatcacccg tctacccaag tcactcaaag ttttgctcga aaacctgctg cgttggcagg
     1561 atggtaaatc ggttaccgaa gaggatatcg acgcgctggc gggatggctg aaaaatgccc
     1621 atgctgaccg tgaaattgcc taccgcccgg caagagtgct gatgcaggac tttaccggcg
     1681 tacctgccgt tgttgatctg gcggcaatgc gcgaagcggt taaacgcctc ggcggcgata
     1741 ctgcaaaggt taacccactc tcaccggtcg acctnnnnnn nnnnttcctg aaattgggaa
     1801 agcaggcgtt cagtcggttt agcgtcgtgc cgccaggcac aggcatttgc catcaggtta
     1861 acctcgaata tctcggcaaa gcagtgtgga gcgaattgca ggacggtgaa tggattgctt
     1921 atccggatac actcgttggt actgactcgc acaccaccat gatcaacggc cttggcgtgc
     1981 tggggtgggg cgttggtggc atcgaagcag aagccgcaat gttaggccag ccggtttcca
     2041 tgcttatccc ggacgtagtg ggctttaaac ttaccggaaa attacgcgaa ggtattaccg
     2101 ccacagacct ggttctcact gttacccaaa tgttgcgcaa acatggcgtg gtggggaaat
     2161 tcgtcgaatt ttatggtgat gggctggatt cactaccgct ggcggatcgc gccactattg
     2221 ccaatatgtc accagaatat ggtgccacct gtggcttctt cccaatcgat gctgtcaccc
     2281 tcgattacat gcgtttaagc gggcgcagtg aagatcaggt cgagttggtc gaaaaatatg
     2341 ccaaagcgca gggcatgtgg cgtaatccgg gcgatgaacc aatttttacc agtgtgttag
     2401 aactggatat gaatgacgtt gaagcgagcc tggcagggcc aaaacgcccg caggatcgcg
     2461 ttgcattgcc cgatgtacca aaagcatttg ctgccagtag cgaactggaa gtgaatgcca
     2521 cgcataaaga tcgcctgccg gtcgattatg ttatgaacgg acatcagtat cagttacctg
     2581 atggcgctgt ggtcattgct gcgataacgt cgtgcaccaa cacctctaac ccaagtgtgc
     2641 tgatggccgc aggcttgctg gcgaaaaaag ccgtaactct gggcctcaag cggcaaccgt
     2701 gggtcaaagc gtcgctggca ccgggttcga aagtcgtttc tgattatctg gcaaaagcga
     2761 aactgacacc gtatctcgat gaactgggat ttaaccttgt gggatacggt tgtaccacct
     2821 gtattggtaa ctctggaccg ttgcccgatc ctatcgaaac ggcaatcaaa aaaggcgatt
     2881 taaccgtcgg tgcagtgctg tccggcaacc gtaactttga aggccgtatc catccgctgg
     2941 ttaaaaccaa ctggctggcc tcgccgccgc tggtggttgc ctatgcgctg gcgggaaata
     3001 tgaatatcaa cctggcttct gagcctatcg gccatgatcg caaaggcgag ccggtatttc
     3061 tgaaagatat ctggccatcg gcacaagaaa ttgcccgtgc ggtagaccaa gtctccacag
     3121 aaatgttccg caaagagtac gcagaagttt ttgaaggcac agccgagtgg aaggaaatta
     3181 acgtcacacg atccgatacc tacggttggc aggaggattc aacctatatt cgcttatcgc
     3241 ctttctttga tgaaatgcag gcaacaccag cacctgtgga agatattcac ggtgcgcgga
     3301 tcctcgcaat gctgggggat tcagtcacca ctgaccatat ctctccggcg ggcagtatta
     3361 agcccgacag cccggcgggt cgatatctac aaggtcgggg tgttgagcgg aaagacttta
     3421 actcctatgg ttcgcggcgt ggtaaccatg aagggatgat gcgcggcacc ttcgccaata
     3481 ttcgcatccg taatgaaatg gtgcc
//
LOCUS       DAWDRC010000433         3505 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10716_length_3505_cov_3.123188, whole genome shotgun sequence.
ACCESSION   DAWDRC010000433 DAWDRC010000000
VERSION     DAWDRC010000433.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10716_length_3505_cov_3.123188"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2388..2397
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          915 a          883 c          822 g          875 t
ORIGIN      
        1 caataaatta aaagcaatca aattgtataa acctttcata aaaacacatg tagcaatttt
       61 ttgttatttc tcatttacta ctacattact gcgtgattga atgattctca attacatatc
      121 cgatggcgaa ataacaaacg gatagtgctt agtgagcaat aagaaacaat tttcactcgc
      181 gacaatccgc gagtttttgg gataacagac taaatggcta tgttcacacc ttcattctca
      241 ggactcaaag gtcgggcgct cttttcactg ctttttgcgg caccgatgat tcatgcaaca
      301 gactctgtaa cgaccaaaga tggcgaaaca atcactgtta cagcagatgc aaataccgca
      361 actgaggcaa ccgatggtta tcaacctctg agcacctcca cggcgacatt aaccgatatg
      421 ccgatgctgg atatcccgca ggtagtcaat acggttagcg atcaggttct ggaaaaccag
      481 aatgcgacaa cgctggatga ggcgctttat aacgtcagta acgtggtaca gaccaataca
      541 ttaggcggga ctcaggatgc ttttgtacgc cgtggatttg gcgcaaaccg ggatggctcc
      601 atcatgacca acggtctgcg aaccgtactt cctcgtagtt tcaacgccgc aacagagcgt
      661 gtggaagtgc taaaaggccc ggcctccacg ctgtatggca ttctcgatcc tggcggactg
      721 attaacgtcg tgaccaagcg cccggaaaaa acattccatg gttcggtttc agccacctcc
      781 tccagttttg gtggcggcac tgggcaactt gatatcacag gtcccattga aggcactcag
      841 ctggcgtatc gtcttaccgg ggaagtgcag gatgaagatt actggcgaaa cttcggtaaa
      901 gagcgcagta catttattgc cccgtcactc acctggtttg gtgataatgc aacagtaacc
      961 atgctctatt cccatcggga ctataaaact ccgttcgatc gtggaacgat tttcgacctt
     1021 acgacgaaac agcccgtaaa cgttgatcga aaaatacgtt ttgacgaacc gtttaatatt
     1081 acagatggtc agtccgatct ggcgcaactc aacgcagaat atcatctcaa tagccagtgg
     1141 acagcgcgct ttgattacag ctacagccag gataaataca gcgataatca ggcgcgtgtt
     1201 accgcgtatg atgcaacgac aggaacactg acacggcgtg ttgatgcaac tcagggatct
     1261 acccagcgta tgcatgctac tcgtgcggat ctgcaaggga atgttgatat tgccggattc
     1321 tataatgaga ttctgggtgg ggtgtcatat gaatattatg atcttctgcg tacagatatg
     1381 attcgctgta aaaaagctaa agatttcaat atctacaacc ctgtttatgg taataccagc
     1441 aaatgtacaa cggtttcggc gtcggacagt gatcagacga tcaaacagga gagctactca
     1501 gcgtatgcac aggacgcact ctatctgacc gataactgga ttgccgtcgc cgggatccgc
     1561 tatcagtatt acacgcagta tgcgggtaaa ggccgtcctt ttaatgtcaa tactgacagc
     1621 cgcgatgaac aatggactcc caaactgggg ttggtctaca aactgacgcc atcggtatcc
     1681 ttatttgcca attattcgca aacatttatg ccgcagtcgt caattgccag ctacattggc
     1741 gatcttccac cagaatcatc taatgcttac gaagtcgggg caaaattcga gctattcgat
     1801 ggtatcaccg cagatattgc gctgtttgat atccataaac gtaatgtgtt gtataccgaa
     1861 agtgttggtg atgaaaccat cgccaaaacg gcaggccgcg ttcgttcaag aggggtagaa
     1921 gtcgaccttg cgggagcatt aactgaaaac attaatatca ttgccagcta cggctatacc
     1981 gatgctaagg ttctggagga tcctgattat gcagggaaac cattgccgaa tgttcctcgt
     2041 cataccggtt cgctattcct gacctatgat attcataaca tgccaggcaa taatacgctg
     2101 acatttggcg gtggtggaca tggtgtaagc cgtcgttcgg caaccaatgg ggctgactat
     2161 tatctgccag gctattttgt tgccgatgcc ttcgccgcat acaaaatgaa attgcagtat
     2221 ccggtcacac tgcaattaaa cgtcaaaaac ctgtttgata aaacgtatta cacctcttcc
     2281 atcgccacaa ataatctggg caaccagatt ggcgatccgc gtgaagtgca attcacggtg
     2341 aaaatggaat tttgatcaaa aaagcccggt ataaacttgt ataccggnnn nnnnnnngta
     2401 taccgggcta tgttttattc ttctggcggg aggattatca gacctcaatc tctgccatgt
     2461 cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg tttcttcgcc agcagcatat
     2521 ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat agtcaactgc accagacggc
     2581 gagtgttcgg atcaagcgtg gtttcgcgca attgcatcgg gttcatttcc ccgagacctt
     2641 taaaacgctg gacgtttggc ttgcctttct tgcgttttaa ttgctcaagt acgccctctt
     2701 tctcttcttc cgtcagcgcg taataaacct ctttcccgag gtcaatacgg tagagcggtg
     2761 gcagtgcaac gtaaacgtga ccgtgtttca ccaatgcgcg gaagtgtttt acaaacaaag
     2821 cgcagagcag cgtggcaatg tgcagaccat cggagtcagc atccgccagg atacagatct
     2881 tgccgtaacg aagctggctc aaatcgtcgc tgtcaggatc gataccgata gctaccgaaa
     2941 tatcgtgcac ttcctgcgaa gccagcactt cgtcggaaga gacttcccag gtgttaagga
     3001 tcttaccttt cagtggcatg atcgcctgat attcgcgatc gcgcgcctgc ttggcagatc
     3061 cgcctgcgga gtcaccttcc acaaggaata gttcggtacg gttaagatcc tgcgcggtac
     3121 agtcagccaa tttaccaggc agcgccgggc cgctggttag ctttttacgc accacttttt
     3181 tagccgcacg catacgacgc tgggcgctgg aaatcgccat ctctgccaac agctccgccg
     3241 cctgaacgtt ctggttcagc cacaggatga aggcatcttt caccacgccc gaaacgaatg
     3301 ccgcacactg acgcgaagag agacgttctt tggtctgccc ggcaaactgc ggatcctgca
     3361 tttttactga cagcacatag gcgcagcgat cccagatatc ttccgccgac agctttacac
     3421 cacgcggcag aatgttgcgg tattcacaga actcacgcat cgcgtccagc aggccctgac
     3481 gcaggccatt aacatgggta ccccc
//
LOCUS       DAWDRC010000434         3501 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10738_length_3501_cov_2.085026, whole genome shotgun sequence.
ACCESSION   DAWDRC010000434 DAWDRC010000000
VERSION     DAWDRC010000434.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10738_length_3501_cov_2.085026"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    469..478
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1134..1143
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1816..1825
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          793 a          910 c          921 g          847 t
ORIGIN      
        1 gcgcaatata ataatcagga attgcgtcat taatacgaat atccagatca atattatcct
       61 gcactaaatc aatttgtcga tcgaacaatt caaaatgcac ctgtaactca ggataattgc
      121 gcatcagttc ggtaatagct ggcgcaatat ggctgcgccc aaaaccaaaa ctgcaaccaa
      181 tgcggatcat cccctccggg cgcgttttga tttgcgtgac gtcatcgacc agtcgctgat
      241 attgggtgag gatttccagc gcatgttcgt aacaacgctg gccactttca gtcagcgcca
      301 cgccgcgagc ggagcggttc agcagcgttg ttgccagcgt attttcaagg atttggatcc
      361 gcttggtgac aaatgcaggc gtttgcccaa gcgtcgccgc tgcagcacta aaactcccgc
      421 tatggacgat ttccaccagt acctgcaagt ctttggctaa aggccagcnn nnnnnnnntt
      481 tcgcaaaaca ttaccattcc ctgctctggc gcaatattcg ttcttatttt cgcgttgatt
      541 cacgaactgt taattacata ttcacggtgg caaaaaatat aaaaccacat ttttagtggt
      601 agtttgtggc ggtgaatttt tccagacaaa tacataaact ggagttgcca tgatgagcga
      661 aagtaataag caacaggcag tgaataagtt gacggagatt gtcgctaact ttaccgccat
      721 gatttctacc cgaatgcctg atgacgtggt ggataaacta aaacagctaa aggatgccga
      781 aacatcgtcg atgggaaaaa tcatctacca cacgatgttc gataacatgc aaaaagcgat
      841 cgacctgaat cgtcctgcct gtcaggacac cggcgaaatc atgttttttg ttaaggtcgg
      901 ttcccgtttc ccactgcttg gcgagctgca aagcatactc aaacaagccg tggaagaggc
      961 aaccgtcaaa gcgccactgc gtcacaatgc ggtagaaatt tttgacgaag taaacaccgg
     1021 caaaaatacc ggcagtggcg taccgtgggt cacctgggat atcgtccccg acggtgacga
     1081 tgcggaaatc gaagtttaca tggcaggcgg cggctgcacg ctacctggcc gatnnnnnnn
     1141 nnngccgatc gaaagtgtta atgccgtcag aaggctacga aggcgtagtg aaattcgtct
     1201 tcgaaaatat ctccaccctc gccgtaaacg cctgtccgcc ggtacttgtg ggcgttggca
     1261 tcgctacctc ggtggaaacc gccgcagtgc tctcgcgtaa agccattttg cgcccgattg
     1321 gcagccgcca tcccaatcca aaagcggcag agctggagct acgcctggaa gaaggactca
     1381 accgtttggg gattggtcca caagggctga ctggcaacag ttcagtgatg ggcgtgcata
     1441 tcgaatctgc cgctcgccat ccgtcaacca tcggcgttgc tgtttctacc ggctgctggg
     1501 cgcatcgtcg cggcacactg cgagttcatg ccgatctcac ctttgaaaat ctgtctcata
     1561 cccggagcgc gttatgaaaa agatcctgac aaccccgatc aaagctgaag atctgcaaga
     1621 tattcgcgtc ggcgatgtga tctacctgac cggtacgctg gtgacctgcc gtgacgtttg
     1681 tcaccgccga ttgatcgaac tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat
     1741 tttccacgct ggccccatcg tgcgcaaaaa cggcgacaaa tgggagatgg tgtccgtcgg
     1801 cccgacaacc agtatnnnnn nnnnnaccag tatgcgtatg gaaagttttg aacgtgaatt
     1861 tatcgagcag accggcgtga aactggtggt cggcaaaggc ggaatggggc cgctgaccga
     1921 agaaggctgc cagaaattca aggcgctgca tgtgattttc ccggctggct gtgcggtact
     1981 ggcggcaact caagtggaag agattgaaga ggtacactgg acagaactcg ggatgccaga
     2041 gtcgctgtgg gtctgccggg tcaaagagtt tggcccgctg attgtctcta tagataccca
     2101 cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt
     2161 ggaagagatt tgcgagcacg tccactacat caaataaccc tcccggagag gctcgcccct
     2221 ctcctttttc gcaggcataa cacgatgaaa ccttccactg aatggtggcg ataccttgcg
     2281 ccgctggcgg tcatcgccat tattgctcta cttccgcttc ccgcagggct ggagagtcat
     2341 acctggctct actttgccgt ttttactggc gtgatcgttg gactgatcct cgaacccgtg
     2401 ccgggtgccg tggtggcgat ggtgggtata tccatcatcg ccattctttc gccgtggctg
     2461 ctgtttagcc cggagcagct cgcccagcca ggttttaagt tcaccgccaa atccctctcc
     2521 tgggcggttt ctggtttttc taattcagtt atctggctta ttttcgccgc ctttatgttt
     2581 ggtacgggct atgaaaaaac cgggcttgga cgtcgcatcg cgctgattct ggtgaaaaaa
     2641 atggggcatc gcacgctgtt tctcggctat gcagtgatgt tctccgagct gatactcgcg
     2701 cctgtaacac cgtccaactc ggcgcgtggt gcggggatta tctatcccat catccgtaac
     2761 ctgccaccgc tctatcaatc acaaccaaac gacagcagtt cgcgcagcat tggctcgtac
     2821 atcatgtgga tggggattgt tgccgattgc gtgaccagcg ccattttcct gacggcgatg
     2881 gctccaaatt tgctgttaat tggactgatg aaaagcgcat ctaacgccac gctgagctgg
     2941 ggcgactggt tcctcgggat gttgccgctc agtattttac tggttctgct ggttccctgg
     3001 ctgacttacg tgctgtaccc gccgatactg aagtctggcg atcaggtgcc gcgctgggca
     3061 gagacggaac tgcaggcaat gggcccgctc tgttcgcgtg aaaaacggat gctggggctg
     3121 atggtaggcg cactggtgct gtggattttt ggcggtgatt atatcgatgc cgcgatggtc
     3181 ggttacagcg tggtggcgct gatgctgctt ctgcgcatta tctgctggga cgacattgtc
     3241 agtaataaag cggcgtggaa cgttttcttc tggctggcct cgcttatcac cctcgctacc
     3301 ggactcaaca acaccggttt tattagctgg tttggcaaac tgttagcagg cagcttaagc
     3361 ggttattcgc caacgatagt gatggtagcg ttgattgtgg tcttttatct gctgcgctac
     3421 ttttttgcca gcgccacagc gtatacctcc gctctcgcac cgatgatgat tgccgccgcg
     3481 ctggcgatgc cggaaatccc g
//
LOCUS       DAWDRC010000435         3495 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10762_length_3495_cov_2.094767, whole genome shotgun sequence.
ACCESSION   DAWDRC010000435 DAWDRC010000000
VERSION     DAWDRC010000435.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10762_length_3495_cov_2.094767"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    635..644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1725..1734
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2708..2717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          903 a          897 c          926 g          739 t
ORIGIN      
        1 gtgccgagca ccacaccgac ctggcggtag gggagatcct gcaattcgtc gtagatataa
       61 ggcgcggttt tccagctcat ccagcgatcg aggccgagca cagtcaacag cagcaagccg
      121 atcaggacta acaggctgag gaacacgcgc tttaacatgc aggagactcg atctaaggaa
      181 aatttccatc aggctacttt acccgctggt tttgcgcaag aaacggcaaa ctgtttgccg
      241 ggattttcga caccaggaaa gggctggcaa gccggacaag gcgttcacgc cgcatccggc
      301 accaggctta agcgagaagc acgcggtcga tattgtgaca gccgagagct ttcagcgttg
      361 cggaggtggc atcccactga atcaacggcg catcggcttt ttctgccaga accgcgcgct
      421 ggatatccgg gtaatcataa cccttgagat tcaacagaat taacgccccc tgcaacggcg
      481 gcagagtcgg gttgaatgcg gcgttttcag cgtagctgcc gctgaaaata cgaccgtctt
      541 tacattccag cgcgacaccg cttggcgact tactgtaagg catgtgcgaa cggtttgccg
      601 ccgcaatcgc cgcctgagaa agcgcatcac cagtnnnnnn nnnngcgcat agccgtgatc
      661 ctgctcgtcc atcagcagcg ttttaatctc cagatctttc ggcccaaagg catccggtag
      721 atagtcacgc agcgcgtgtg cctcgcgtcc cggaagatga atacgcagat ccagaccgct
      781 gttcagttca ttcataaact gacggcagtg accacaaggc gtgtagttaa cggtgatggc
      841 tgcaagcgct ttttcaccac tcagccaggc gtggctgatc gcgctttgtt cggcatgaac
      901 ggtttgctgc attgtcgcac caataaattc catattggca ccgaaatacc aggttccgct
      961 cacaccgcgc gcaattgcgc caacattaaa attcgacaat ggcgtacgcg cacaggccgc
     1021 cgccagcgga agtagtgcga atgccagcgc gtcttcgtcc agccccgttg cgctcttcag
     1081 cgatgagact tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca gaataggttc
     1141 cagtgcagat tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac gtggatgcat
     1201 ggcgttgcct cataatcatg taatgggata acagtttacg aacccgctat ggctttatat
     1261 gtgatctgaa tctcattaat tatgcaacgc gtgaaactta catcaaaaat gcgcgacgca
     1321 tcgcaaattt tagcccatta ctgccagaat aatcgggaaa aggaacggtg cgatcagcga
     1381 agtaataatc ccgcataaca ccagcgccag cgaactaaat gcaccttcct gataatccag
     1441 ctcggcgcag cgcgccgtac cgagggcgtg cgaggctgtg cccattgcca gaccgcgcgc
     1501 agctttggta cgaatacgca tcgcattaag caatgtatgg ccaaacacag cgccgaggat
     1561 gccgacgaaa atcacgcaaa ctgcgctgat tgccggaata ccgccaatac tgccgccaac
     1621 cgccattgca ataggcgtgg taactgattt cggcaggatt gacgcggcga tttccggtga
     1681 agcgcccatc aacaatgcca cagaagtccc cgtcaccatc gcaannnnnn nnnncgagcg
     1741 gatctggtgt agctgctcat ataaaggata agccagtgcg acgaccgccg gttgcagcag
     1801 atcgtttagc acttcgctac ctttaaagta gctgtcgtaa gagatgccag tcagcattaa
     1861 aaaaggaatg atcaccacca ttgctaccag caacgggtta agcaggggaa acttataccg
     1921 tgccgccagt ttgcgggcgg caaaaaagac aatcaacgtc agcggtaatg accaccagat
     1981 attcgccatc attcttctga tcctttctga cctacgactt tacgttcacc gtgcaccagt
     2041 tgtgaactcc agctcaccac cagaaaaacc accagcgtac tgactgcaca ggaaaccact
     2101 accggaccaa actgtgcgcg gagcaaatca aaatattgca tgacgcctac accaatcggc
     2161 acaaacaata gcgccatata gcgaatcagt acgtagcacc ccggattgac ccatttcgcc
     2221 ggaagaattt gcaaggccag caggacaaac aggatcagca tcccgatgat gctgcccgga
     2281 atggttaccg gtagcaggga agcaatgaaa atgcctgcat acaggcaggc ataaatcagg
     2341 acgaaagcgc gtaaatattg ccagataatg ttcagtgtct tactcataat caacacccca
     2401 gatgagatgg attcatcata caattaattc gtaaaacgtg ctaccgatca cattaagaaa
     2461 tcagcgctta ttttttcgct ccccggtatt acgcaatgcg cagcgaactc aggcatacat
     2521 caagcataga gagggattgc agtttgcagc aatccctctt tgcagacctt taatccgttg
     2581 accggaggcg ctaacatagc gcctcatttt tttgcgggtg atgatatgcg tgtgttactg
     2641 gctccgatgg agggtgtact tgactctctg gtgcgggaat tactgaccga agttaacgac
     2701 tacgatcnnn nnnnnnnaaa atgccagccg gacaccatcc ggtacgctgg ttcgcgtgca
     2761 gttgttaggg cagttcccgc agtggctggc agagaacgcc gcccgtgcgg tggagttagg
     2821 ttcctggggc gtggatctta actgcggctg cccgtcgaaa acggttaacg gtagcggtgg
     2881 tggggcaacg ttactgaaag atcctgaact catctaccag ggtgcaaaag cgatgcgcga
     2941 agctgtaccg gcgcatttgc ctgtcagcgt aaaagtgcgt ctgggctggg acagcggtga
     3001 gaagaaattt gaaatcgccg atgcggttca acaggctggt gctacggagc tggtagtaca
     3061 tgggcggaca aaagagcagg gttaccgcgc ggaacatatt gactggcagg cgattggcga
     3121 gattcgccag cggcttaata ttccggtgat tgccaacggt gaaatctggg actggcagag
     3181 tgcgcaacaa tgcatggcga tcagcggctg cgacgcagtg atgattggtc gcggggcgct
     3241 caatattccc aacctgagcc gtgtggtgaa atataacgaa ccgcgaatgc cgtggccgga
     3301 ggtggttgct ttgctgcaaa aatatacccg tctggaaaag cagggcgata ccgggttata
     3361 tcacgttgcg cggattaaac agtggttgag ttatttgcgt aaagaatacg atgaagcaac
     3421 ggaattattt cagcatgttc gggtgttgaa taattcccct gatattgcaa gggctattca
     3481 ggccattgat atcga
//
LOCUS       DAWDRC010000436         3481 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10818_length_3481_cov_1.956509, whole genome shotgun sequence.
ACCESSION   DAWDRC010000436 DAWDRC010000000
VERSION     DAWDRC010000436.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10818_length_3481_cov_1.956509"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    683..692
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1398..1407
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2381..2390
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          813 a          900 c          969 g          769 t
ORIGIN      
        1 atgaataact taaagaacat tcagataatt ttacctgctc ttttagtgtt aaattctgat
       61 gagaaaacaa accagcagaa atatctctgg cgaattaatc gctatataaa tatgtttata
      121 accatttgaa atgtgagcaa aagcccgttt ttgccacgct tcccgccact cttttgatcc
      181 tgctacaggt tttaccccga tcggggtatg catctttgac acatccttta atatcttagc
      241 ggctataaaa atggcttatt aattatgcgg tttatttggt cgctctcaat tttcagagcg
      301 cgttaatgat ggaaggtcaa tgtgaagatt gatgcatccc gtcggggcat actcactggt
      361 cgctggcgca aagccagtaa cggtatccgt ccgccctggt ccggtgatga atctcatttt
      421 ctgacccatt gcacccgctg cgacgcctgt attaatgctt gtgaaaacaa cattctgcaa
      481 cgcggcgcgg gtggctatcc gagcgttaat ttcaaaaata acgagtgcag cttctgttac
      541 gcctgcgcgc aggcttgccc cgaatcgcta ttttctccgc gccacaccag ggcctgggat
      601 ttgcagttca ccattgggga cgcgtgcctg gcgtatcagt cagttgaatg ccgccgctgt
      661 caggatagct gtgagccaat ggnnnnnnnn nnttatcttt cgcccgacgc tctccgggat
      721 ctaccagccg caacttaata gccaactttg caacggatgt ggagcatgtg cagccagctg
      781 tccggtatca gccataactg cggagtatct ccatgcacac taactggcaa gtttgcagcc
      841 tggtcgtgca ggccaaaagc gaacgaattt cagacatcag cacccaactg aacgccttta
      901 ccggctgtga agttgctgtc agcgacgcgc cgagcggtca gttgattgtg gtggtggaag
      961 cagaagacag cgaaacgctg atccaaacca ttgagtcagt acgcaacgta gagggcgtgc
     1021 tggcggtgtc gctggtttat caccagcagg aagagcaagg tgaggaaaca ccatgaaact
     1081 cagtcgtcgt agctttatga aagctaacgc cgttgcggcc gctgcggcgg ctgccggtct
     1141 cagcgtgccg ggcgttgccc gcgccgttgt tggtcagcag gaagccatta aatgggataa
     1201 agcgccgtgc cgtttctgcg gtacgggttg cggcgttctg gtcggaacgc agcaggggcg
     1261 tgtggtggcc tgtcagggcg acccggacgc accggttaac cgtggcctga actgcattaa
     1321 gggctatttc ctgcccaaaa tcatgtacgg taaagaccgt ttgacgcagc cgctgctgcg
     1381 tatgaaaaac ggtaaatnnn nnnnnnngat aaagaaggcg aatttacccc aatcacctgg
     1441 gatcaggcct tcgatgtgat ggaagagaaa ttcaaaaccg ccctgaaaga aaaagggccg
     1501 gaatcgatcg gtatgttcgg ttctggtcag tggactatct gggaaggtta tgccgcgtcc
     1561 aagctgttta aagcgggctt ccgttcgaac aacatcgacc cgaacgcgcg tcactgtatg
     1621 gcgtcggcag tagttggctt tatgcgtacc tttggtatgg atgagccgat gggctgctac
     1681 gacgacatcg agcaggctga cgcgtttgtg ctgtggggcg caaacatggc ggagatgcac
     1741 ccgatcctct ggtcgcgcat cactaaccgt cgtctttcta accagaacgt caccgtggcg
     1801 gtgctttcta cctaccagca tcgtagcttc gagctggcgg ataacggcat catctttacg
     1861 ccgcaatctg acctggtgat cctgaactat atcgccaact atatcattca aaacaatgcg
     1921 ataaatcagg acttcttcag caaacacgtt aacctgcgca aaggggcgac ggacatcggc
     1981 tacggtttac gtccgaccca tccgctggaa aaagcagcga agaatccggg ttctgacgcc
     2041 tccgaaccga tgagctttga agattacaaa gccttcgttg ctgaatatac gctggaaaaa
     2101 accgccgaaa tgaccggcgt gccgaaagac cagttagaac aactggcgca gctgtatgcc
     2161 gatccgaaca agaaagtcat ctcctactgg acgatgggct tcaaccagca tacccgtggt
     2221 gtatgggcca acaacctggt ctacaacctg cacctgctga ccggcaaaat ttcccagccg
     2281 ggttgcggtc cgttctccct gaccgggcag ccttccgcgt gtggtactgc gcgtgaagtg
     2341 ggcacctttg ctcaccgtct gcctgcggac atggtggtga nnnnnnnnnn cacgcggtgg
     2401 cataagaccg tgcggtgaaa gacggcaaac tgaatgttta ctggaccatg tgtaccaata
     2461 acatgcaggc cggaccgaac attaatgaag agcgtatgcc gggctggcgc gatccgcgca
     2521 actttatcat cgtttccgat ccgtatccga cggtcagtgc gctggcagcg gacttgatcc
     2581 tgccgaccgc aatgtgggta gagaaagagg gcgcttacgg taatgccgaa cgccgtactc
     2641 agttctggcg tcagcaggta caggcaccgg gtgaagcgaa atcggatctc tggcagttag
     2701 ttcagttctc ccgccgcttc aaaactgaag aagtgtggcc ggaagagttg ctggcgaaga
     2761 aacctgaact gcgtggcaaa acgctgtatg aggttctgta tgccactccg gaagtgagca
     2821 aattcccgct atccgaactg gcggaagatc agctgaacga tgaatcccgc gagctgggct
     2881 tctatctgca aaaagggctg ttcgaagagt acgcatggtt tggtcgcggt cacggtcacg
     2941 atctcgcgcc gttcgatgac taccacaaag cacgcggtct gcgctggccg gtggtgaacg
     3001 gtaaagaaac gcagtggcgt tacagcgaag gtaacgaccc gtacgtgaaa gcgggcgaag
     3061 gctacaagtt ctacggtaaa ccggatggca aagcggtgat cttcgcgctg ccgttcgaac
     3121 cggcggcgga agcaccggat gaagagtacg acctgtggct ctctaccgga cgcgtcctgg
     3181 agcactggca taccggcagt atgactcgcc gtgtaccgga actgcaccgc gccttccctg
     3241 aagcggttct gtttattcac ccgctggatg cgaaagcgcg cgatctgcgt cgtggcgaca
     3301 aagtgaaagt ggtttctcgc cgtggcgaag tgatctcgat tgtcgaaacg cgcggtcgta
     3361 accgtccgcc gcagggcctg gtgtacatgc cgttcttcga cgccgcacag ctggttaaca
     3421 aactgacgct ggatgcgacc gatccgctct cgaaagagac ggacttcaag aagtgcgcgg
     3481 t
//
LOCUS       DAWDRC010000437         3478 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10826_length_3478_cov_2.749343, whole genome shotgun sequence.
ACCESSION   DAWDRC010000437 DAWDRC010000000
VERSION     DAWDRC010000437.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10826_length_3478_cov_2.749343"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1142..1151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2619..2628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          815 a          933 c          824 g          886 t
ORIGIN      
        1 cataatcatt cactaagtta atttatatag tatctgccca gacacttatt tatagttatt
       61 aaaggcgcgt ccgactggtt caccggacgc accttaagta cgtttccttg tgttataaga
      121 acaaaaggag cagctgtaaa acagcaatga tgattttgat gacccgttta atcaggtatc
      181 ggcaatcagt cattcgtttt tccttaaaca aggaatgcag ccgttcagat tagcccttac
      241 atctccccaa aactgaacgt gcgagttatt ggaatcgagt gctgcactcc acaccagagc
      301 tttgacgaca ccactcgttt caatggggga attcagtggc atggtgtaaa gcacagcaaa
      361 attgccaata acgaaaccga ttttaatgta cttatcaaaa agcggtcacg ttaatctttt
      421 catcaatgtc gatttcttcg taaaggctcg cgttttttat gtcagagatt gcaagttgct
      481 tgtacaggtc aacgaaattg atccataatc attttcgttg gaatcgtgtg ctgcacaaaa
      541 ttaaagttaa aaagtaaaac ccccgttcct taccagttcg ggggttttac tttataaaga
      601 gaacggtatt attttaactt tcaataatta ccgtggcaca agcatagtgg cgctcatcgg
      661 caagcgttac atgcatattt acaacgccga gcttttccgc cagttttaat gcctcacccc
      721 atagccgtag ccgtggtttg ccgagctcat cattgaatac ttcaaactga ttaaacgcca
      781 gaccattgcg gatccctgtg ccaaatgctt ttgccgccgc ttctttcaca gcaaaacgct
      841 tcgccagaaa acgcactggc tggtggtgcg ttttccagat ttcccattcg ttatcgctta
      901 atacgcggcg tgccaggcgc tcaccggatc gggcgatcac cgcttctatg cgagcgatct
      961 ctacaatatc cgtacctaaa cctaatattg ccattagcca cgcgcttcca gcatcagacg
     1021 tttcatttct gccaccgcat ctttcagtcc ggtcatcact gcacgaccaa taatggcatg
     1081 accgatattc agttcatgca tctcagggat ggcggcaatg gctttcacgt tgtgataggt
     1141 cnnnnnnnnn ncggtgtgga tctcgataaa cggtgcgcca acctctgccg cagctttgat
     1201 ctgctcttca tcggcgtcga taaacagaga aacctggatc ccggcatctg ccagacgttt
     1261 gcaggcatcg cgcatttttt cacgctgccc tgcgacgtcc aggccgcctt cggttgttac
     1321 ttcctgacgc ttttccggta ccagacagca aaaatgtggc ttcgtctcaa cggcgatcgc
     1381 cagcatctct tcggtcaccg ccatctccag attcatgcgg gtatccagcg tctgacgcag
     1441 gatgcgcaca tcacggtcag taatgtggcg gcgatcttca cgtaaatgca cggtaatgcc
     1501 gtccgctccc gcctgctcag caataaacgc cgcctgcacc ggatccgggt aagcagtacc
     1561 gcgtgcgtta cgcagcgttg caatatggtc aatgttgacg cctaacagta attcagccat
     1621 gacaatcctc atcattcata atgtgttttc accgttcgct taggcataaa ctgccggaac
     1681 agttccctgc tctttaatgg tttaccgcca agatacggct taagcgccat gcgggtaaag
     1741 cgtttcgcgg cgcgcagtgt gtctgcgtca ggaaattccc gcgcgtttaa cgcttttaac
     1801 tgccttccgg tgaacgtttt attgtcgata acgacgcttg cgataaaccc tttttcttcg
     1861 cgataacgat acgtcatggt gtcatctacc ggctcgccgc tacccgcaca atgggtaaaa
     1921 ttgacgccat agcccagatg cccgagcagt gccagttcaa agcggcgcag cgcgggttct
     1981 ggcgtaccag tggcacctgc aagtgactga atgcagtgca agtaatcgaa aaagagttca
     2041 gagaagcgcg tctcgtattc cagtacgcgg gagagaagtt cgttgatgta cagaccgctg
     2101 taaagcgtga taccgcttaa tggcagcgcc agcgagacag cttcagcact gcgcagcgtt
     2161 ttgacttcgc cacgcccgcc aaaacgtagc aagagagggg tgaaaggctg taatgcccct
     2221 ttcagagtag agcgtttaga gcgtgcgcct ttggcaacca gacgcacgcg acccgattcc
     2281 tccgtgaaga cgtccagcat caggctggtt tcgctccacg ggcgactatg caggacaaat
     2341 gcgcgctgcc agccttccat cggagttact cttaaagatc gtcaacgtaa ccgagactgc
     2401 gcagtgcgcg ttcgtcgtcg gcccaaccgg atttcacttt tacccacagc tcaaggtgaa
     2461 caggcgcttc gaacatttcc tgcatgtctt tacgcgcttc aatcccgatg gttttgatct
     2521 tggccccttt gttgccaatg accatcttct tctgcccttc acgctcaacg agaatcaaac
     2581 cgttgatgtc ataaccgccg cgttcgttag agacgaaann nnnnnnnnga aacgttcgat
     2641 ctccacggtc acggagtacg gcagttcagc gccgagaaaa cgcatcagtt tttcgcggat
     2701 aatttcagac gccataaaac gctgtgagcg atcggtgatg taatcttccg ggaagtgatg
     2761 agtcgcttca ggcagatgct tacgcacaat tgccgcaata gtatcaacat tcaacccggt
     2821 ttcggcagag atcggcacga tatcgaggaa gttcatctgg cttgccagga actgtaagtg
     2881 tggcagcaga tcggcttttt cctgcacgtt atccactttg ttcaccgcga ggattaccgg
     2941 cgctttgcca tcgcgcagtt tgttgagcac catttcatcg tccggcgtcc agcgggtgcc
     3001 ttctacgaca aaaatcacca gctcaacgtc gccgatagag ctgctcgccg ctttgttcat
     3061 caggcggtta atggcgcgtt tttcttccat atgcaggcca ggggtatcaa cgtagatcgc
     3121 ctgatacgcg ccttcagtat ggatccccac aatgcggtga cgagttgttt gcgccttgcg
     3181 ggaggtgatg gagatcttct gccccagcag tttgttcaat aatgtggatt tgccaacgtt
     3241 cggacgtccg acgatagcaa taaatccgca gtaactttta tcgatgctca ttccagctcc
     3301 agttttttca acgcctgttc ggcggcagcc tgctcagcct tacgacggct tgaacctgtg
     3361 ccaaccaccg gttcactcag gccgctgacc tggcagtgga tagtaaattc ctgatcgtgt
     3421 gcttcgccac gtacctggac gaccagataa gtcggcagcg gcagatggcg accctgca
//
LOCUS       DAWDRC010000438         3471 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10846_length_3471_cov_2.811183, whole genome shotgun sequence.
ACCESSION   DAWDRC010000438 DAWDRC010000000
VERSION     DAWDRC010000438.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10846_length_3471_cov_2.811183"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    3191..3200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          831 a          874 c          920 g          836 t
ORIGIN      
        1 gtaatgcttt gctgagcagc tgcgccgcgc cacgtaaacc gccaagcgga tttttaatct
       61 catgtgccag gccgcgcact aaatcacggg cagcaacctg ctgggcgtgc tgtagctgtt
      121 cctgacttaa gcggcgctgg ttatccatcg gtgccatctc cagcaggatc atgccgtccg
      181 gcatacgctg agccgtcaca gaaaggatat gcgagcgccc gtcgatgacc agcgtcactt
      241 cgttatcggt aaaaccttgc cccgcctcca gactttcttg catcagctcg atatttaatg
      301 agaagtagct caacagttcc ggtaacggtg taccaaacaa tttgcgggag ctttgggcga
      361 gcagttgttg cgcggcgggg ttggcgtaat ggatcgccag gttgtcatcg attaacaaaa
      421 tactgttaat cagcgagttg aggatctgcc cagcatcggg ctgcgtgcct gttgccataa
      481 agcagtctcc tgaacaggtt gcaccatttt agtgcattat agctttttac ggataaaaag
      541 cgcgaagcat cagagaattg acggagaaaa aagcccatgc agagatgggc tgaaagtttc
      601 cacggcaact aaatcccggc gttgttgcgc cgggtagtac aacttcaact attagacgct
      661 gtagtacagc tcaaactcta ccggatgcgg agtcatacgc acgcggtcat cttcttcgcg
      721 acgcagagcg atgtacgcat cgatcgcttc atccgtgaac acgccaccgg ctttcaggaa
      781 ctcgcgatcc agatccagtt cgttcagtgc ttcttccaga gagcctgcaa cctgtgggat
      841 ctctttcgct tcttctggcg gcaggtcata caggtttttg tccatggctt cgcccggatg
      901 gatcttgttc ttgatgccat caagaccggc catcagcagg gcagcaaagc acaggtacgg
      961 gttagccgcc gggtccggga aacgtacttc gatacgacgt gctttcggag aagaaaccac
     1021 cggaatacgg atagacgcag aacggttacg cgcagagtaa gccagcatta ccggtgcttc
     1081 gtagcccggg accagacgct tgtaagagtt ggtggtcggg tttgccaggg cgttaatcgc
     1141 tttagcgtgt ttgattacgc cgccaatgta gtacagtgcc tgttcagaca gacctgcgta
     1201 tttgtcgcca gcaaacaggt tgacgccgtt tttagacagc gacatgtggc agtgcatacc
     1261 ggaaccgtta tcaccgaaca tcggttttgg cataaaggtc gcggtcttac cgaaacggtg
     1321 cgccacgttg tgcacgacat atttgtagat ctgaatttcg tcagcttttt tggtcatggt
     1381 attgaagcgg gtagccactt cgttctgacc tgcggtagcg acttcgtggt gatgagcttc
     1441 aaccaccaga cccatctgtt ccatcaccag acacatttca gaacgaatat cctgagctga
     1501 gtctaccggc ggaaccggga agtaaccgcc tttcactgcc ggacggtgac ctttgttacc
     1561 accttcatat tgggtggagg agttccatgc accttcgata tcgtcgatag caacgtggga
     1621 accggagata gacgatccga aacggatgtc atcgaacagg aagaattcag gttctggccc
     1681 gaacagtacg gtgtcggcaa tgccagtaga acgcaggtaa tcttcagcgc gcttcgcaat
     1741 agagcgcggg tcacggtcat agccttgcag ggtgccaggt tcaaggatgt cgcaacggat
     1801 aatcagggta gaatcggcga agaacgggtc aatcactgcg gtggatgcgt ctggcatcag
     1861 caccatatcg gattcgttaa tgcctttcca gccgccaatc gaggagccgt caaacatttt
     1921 gccttcttcg aagaattcgg cattcacctg atgagcaggg atagtgacgt gctgttcttt
     1981 acctttggta tcggtgaagc gcaaatcaac aaacttcact tcgtgctcgt tcagcatcgt
     2041 cagtacgtgt tcagcggaca tactttaact ctcctggatt ggtcattgtc gtcgtggtaa
     2101 cgaaatctgc aattaaatgg gccgtgtcgc cgtaaaaaag ataaagcgaa atctgtgcca
     2161 acttttaaat tgcccctaaa aggcgttatc atgcttaaca tcgtgcaaaa gggctgcacc
     2221 atgatgtgaa tgttgcacca atatagtgct tcaatgggaa cattaagcac catattggtg
     2281 caatgacctt tggataaccc tttttatgct ccgtgaaagc gatcacaaag ggactctgca
     2341 atacttgttt gcggaggatg tttgtgatcc tgttttgtag tgcgattaat ccgtgtacaa
     2401 taacgcgcta tttctaatgc ctgaggcaaa gttgtgatcg aaaaattgcg taatatcgcc
     2461 atcatcgcgc acgtagacca tggtaaaacc accctggtag acaagctgct ccaacaatcc
     2521 ggtacgttcg actctcgtgc cgaaacccaa gagcgcgtga tggactccaa cgatttggag
     2581 aaagagcgtg ggattaccat cctcgcgaaa aacaccgcta tcaaatggaa tgattaccgt
     2641 atcaacatcg ttgatacccc ggggcacgcc gacttcggtg gtgaagttga acgtgtaatg
     2701 tccatggtag actcagtgct gctggtggtt gacgcatttg acggcccgat gccgcaaacg
     2761 cgcttcgtaa ccaaaaaagc gtttgcttac ggcctgaagc cgattgttgt tatcaacaaa
     2821 gttgaccgcc ctggcgcgcg tcctgattgg gttgtggatc aggtattcga tctgttcgtt
     2881 aacctcgacg cgaccgacga gcagctggac ttcccgatcg tttacgcttc tgcgctgaac
     2941 ggtatcgcgg gtctggacca cgaagatatg gcggaagaca tgaccccgct gtaccaggcg
     3001 attgttgacc acgttcctgc gccggacgtt gaccttgacg gtccgttcca gatgcagatt
     3061 tctcagctcg attacaacag ctatgttggc gttatcggca ttggccgcat caagcgcggt
     3121 aaagtgaagc cgaaccagca ggttactatc atcgatagcg aaggcaaaac ccgcaacgcg
     3181 aaagtcggta nnnnnnnnnn gcggggccac ctcggtctgg aacgtatcga aaccgatctg
     3241 gcggaagctg gcgatatcgt ggcgatcacg ggtcttggcg aactgaacat ttctgacacc
     3301 gtttgcgaca cgcaaaacgt tgaagcgctg ccggcactct ccgttgatga gccgaccgtt
     3361 tctatgttct tctgcgttaa cacctcgccg ttctgcggta aagaaggtaa gttcgtaacg
     3421 tctcgtcaga tcctggatcg tctgaacaaa gaactggtac acaacgttgc g
//
LOCUS       DAWDRC010000439         3463 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10876_length_3463_cov_2.105047, whole genome shotgun sequence.
ACCESSION   DAWDRC010000439 DAWDRC010000000
VERSION     DAWDRC010000439.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10876_length_3463_cov_2.105047"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    590..599
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2336..2345
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          834 a          953 c          842 g          814 t
ORIGIN      
        1 atatctttaa ttctgaccgg caagcccgct ttacaagcag taacataagc aataccgccc
       61 cccatcaagc caccaccgag aatccccacg ctgtttaatg gcgcaggcgg cgcatcactg
      121 ccgggatctt ttttcacttc cgtactggca aaaaagatat tgcgcagcgc ctgcgattgc
      181 ggcgtcatcg ccagttcgcc aaacgcccgt gcttcagcgt catagccgct gctggtgccc
      241 tgcgctaatc ccgtttcgac aacctccagg atgcgttctg tcgccggata gttaccttgc
      301 gttttgtgtt ctgttttctt accgaccatt ttgaacagca gcgcacgacc taacggcccc
      361 gccagaatac gctcgcgtac aggtagaggg cgtgaagatg ggcgatcctg ctttgccagc
      421 tcaacagcgg cttccagcaa tatggagtgc ggaacgacgt catccaccaa ccccaacttt
      481 accgcctgtt tcgcccgaag ttgttttccg gtgaggatca tctctaatgc tgtgctgaca
      541 cctatcaggc gcggtaaacg ctgggtaccg cctgatccgg gtaacaatcn nnnnnnnnng
      601 ataaccggaa tgggcaaagc atgaatctcc gccattaact gttgcccctg ccgcgccagt
      661 acttccgctt cttgcgccgt tttgcagttg ccgatcatgt tgatgtctgc gccagcaatg
      721 aagttgtctg gtttcgcgga gacaaacacc acgccacgca actctttgtt ttcacggatt
      781 tgcttaataa tggcgcgtac ctgcgaggca aattccgcct tgagggtatt cattttctca
      841 cccggtacgt cgatggtgat gatagcaatg ttgtccagac gaacattaag agtaaacgct
      901 gatgccattt ccattattcc gcctccagaa ccattgctgc gccaagccca cccgcagcac
      961 aggcggtcac taaaccaaat ccaccgccgc gacggcgaag ttcatgcaat gtctgggtaa
     1021 tcatccgcgc gccggtcgct gcgaagggat gtccataagc aatcgaaccg ccaagcacgt
     1081 taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagtacg tcgcgagcaa
     1141 aacgttcgct gccaagcaac tgaatattgg ccagcgtctg ggcggcaaag gcttcgtgca
     1201 tatcgatcaa tgtcagatcg ctcatcgtca atccggcacg ttccagcgcc agcggtgttg
     1261 accaggctgg accgagcaac atgtcctgcc agacatctat cgcagtaaat gcgtagctgc
     1321 gcagataccc cagcggtacc agccctaatt ctttcgcccg ggattcagtc atcaggatca
     1381 ccgctgccgc gccatcggtc agcggcgtac tgttcgccgc cgttaccgtc ccgtgtttac
     1441 gatcaaacgc cggacgcagc tttgcgtaat cagcaagaga ggaattaccg cgaatattgt
     1501 tgtcttcgac aagcggttgt ttataagggg ggataaaggc agtcatcacc tcttctttga
     1561 gtttaccttc tgaccacgcc tgagcggcac gctgatgcga acggtgcgcc agtgcatctt
     1621 gctgttctcg ggtgatgccg taggttttcg ccatttgctc tgcggtgtcc cccatccgta
     1681 atccagtgga gtattccgcc actgccggag gcaccggcat taagtcacgc aaacgtaagc
     1741 gagaaaaaag tttcaggcgc tggctcattg tgcgggcttt gttgacatca accagtacgc
     1801 gcgccagttt tttactgacg ccaattggca atacggaaga ggaatctgcc ccaccggcaa
     1861 tccccgctcg aatagttccc gccatcaggc tttctgcgac gtttgcaacc gcctggaaac
     1921 tggtagcgca agcgcggcta acgctgtaag catcggtatg cacattcatt ccagtaccga
     1981 gaacaatttc acgggcaatg ttgggggctt caggcatctg aacgacctga ccgaagacca
     2041 gttgttcaat cacttcagcg gggatctcgg tgcgtgccag cagttcgcct accaccatct
     2101 tccctaaatc aaccgcggga atgccatgaa aagccgtcgc ctgtcgggca aaaggcgtac
     2161 gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagcggt aaaacctgac
     2221 ccataacact cccctgtaaa taaagtggtc tgacctgatc atagtcttaa ccattttttt
     2281 acatttagcc aagtggagaa aagggaaagt gggagctatg acacagagag aaaagnnnnn
     2341 nnnnngatga ctaaatatga cagaaaagtc gctaaaagaa aagaagaaga gaaaagaaaa
     2401 cgcccctgcc agcaactgac aggggcgtat gtacccgaga ggaattaacg cagacccagc
     2461 tggaagatca gcatttcagc ttcgcaggca aaagtgaaat cgatatccag gcgcacaccg
     2521 tcagactctt cagtgaaagt cggggtgatt ttgcacggtt cggattccac gctacgggct
     2581 ttttcagtca gcgccgccag cgtttgttct gcttctgcgc ggtttgcaaa cacacggctg
     2641 taagacgcgg tgcagtcgga gttgtccata atggtgccaa catccataca gcagcaaacc
     2701 ggggtttcat cagcactaca tttactcatc gttgatttcc tctgtatgtg cacccaaggt
     2761 gccagataaa cgttgtggat attttacgct tccggaaagt gctgctccag ttgttaattc
     2821 tgcaaaatcg gataagtgac cgaaatcaca cttaaaaatg atctaaaaca aaattcaccc
     2881 gaatccatga gtgcgccacc tcccaatttt gccagctgga tcgcgtttct tagatcatat
     2941 ctgaaaaaag atagaaacat acttgcaaca ttccagctgg tccgacctat actctcgcca
     3001 ctggtctgat ttctaagatg tacctcagac cctacacttc gcgctcctgt tacagcacgt
     3061 aacatagttt gtataaaaat aaatcattga ggttatggtc atgagccaga aaaccctgtt
     3121 tacaaagtct gctctcgcag tcgcagtggc acttatctcc acccaggcct ggtcggcagg
     3181 ctttcagtta aacgaatttt cttcctctgg cctgggccgg gcttattcag gggaaggtgc
     3241 aattgccgat gatgcaggta acgttagccg taaccccgca ttgattacca tgtttgaccg
     3301 cccgacattt tctgcgggtg cggtttatat tgatccggat gtaaatatca gcggaacgtc
     3361 tccgtctggt cgtagcctga aagccgataa catcgcgcct acggcatggg ttccgaacat
     3421 gcactttgtt gcaccgatta acgaccaatt tggttggggc gct
//
LOCUS       DAWDRC010000440         3445 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10936_length_3445_cov_2.422124, whole genome shotgun sequence.
ACCESSION   DAWDRC010000440 DAWDRC010000000
VERSION     DAWDRC010000440.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10936_length_3445_cov_2.422124"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2455..2464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3023..3032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          857 a          928 c          889 g          751 t
ORIGIN      
        1 cctcccgcga aagggaaaga ggtggcggtg gatgacggta ttctcggtgg attgaaggat
       61 attttgtttg gcactaccgg accacgcggc gggaagaaag atggtgtggt gcaaacaatg
      121 gcaaaaagcg ccgcccgcca ggtgacgaat cagattgtgc gcgggatgtt ggggagtttg
      181 ctggggggga gaagaaggta agccaggtgc gatttgtctt tgcctgatgc gacgctggcg
      241 cgccgcatca ggcctacact actgcaatat attgaatttt catgatcttt caggcatgaa
      301 cgaaacacac tttgctaaca ataataaagg gagctttcgc tccctttatt cgttcattcg
      361 gttacgattt tctcattaac agccacaggc tgattaagaa gaagctggcg cttggcaaca
      421 gtgcgccgat gatcggcggg atgccgtaaa ccaacgtcag cgggccgaag atctggtcca
      481 gtacgtagaa gacaaaaccg aagctaatac cagtgaccac acgcacgccc atcggtacgc
      541 tacgcagtgg gccaaagatg aacgacagcg ccatcagcat catcaccgcc acggatagcg
      601 gctggaagat tttgctccac atgttgagct gataacgtcc ggcatcctga ccgctcgact
      661 tcagatactt cacatagttg tgcaaaccgc tgatagagag tgcatccggg tccagcgcca
      721 ccacgcccag tttgtctggc gtgaggttgg ttttccaggt gccgctcacc gtctgcgaac
      781 cggtgatctg tttcggattg gtcaaatcag attcatcaac ctgcgacaga cgccagactt
      841 tatgttccgg gtcaaacttc gcggcagcgg catagcgtac ggattgcaga cgacgattct
      901 cattaaaggc ataaatactg atgccaccta actcttcgtc acctttaacc cgctcaatgt
      961 agacgaagtt gttgccatct ttcgcccata aaccttgctg ggtagagagc aacgagccgc
     1021 cgtacatcgc ctgcgcacgg tagttacgcg ccatctgctc gccctgcggc gcgacccatt
     1081 caccaatcgc catcgtcagt aagaccagtg gaatggcggt tttcatcacc gacagcgcta
     1141 cctgcatacg ggtaaaacca gaagcctgca tcaccaccag ttcgctgcgc tgcgccagca
     1201 tcccaagacc aagcaacgcc ccaagcagag ccgccatcgg gaagaagatt tgcacatctt
     1261 tcggcacgct cagcaaggta tacattcctg cgcctaacgc gtcgtaactc ccctgcccgg
     1321 cttttttcag ctgatcgaca aacttgataa tgccggaaag cgacaccagc atgaacagcg
     1381 tcatcatgat ggtggtgaaa atagttttac cgatatagcg gtcaagtacg ccaaaaggtt
     1441 gcatcacacc gctcctttac gcgaaaaact ggcgcgcagg cggcggaccg gcacggtgtc
     1501 ccaaaggttg agaacaatcg ccaaagccag ataaatcagg ttaacggtcc acatccacag
     1561 cgtcgggtcc agcttacctt taccgccgtt cgatttcagg gaggtctgga tcaggaagaa
     1621 caacagatac agcagcatgg ctggcagcat cgatagtacg cgcccctgac gcgggttaac
     1681 cacgctcagc ggtacgacca taagtgccat cataaacacg gtgaacacca acgtgatacg
     1741 ccagttcagt tctgcgcgag cacgatcggt gtcggtgttc cacaatgtgc gcatgtccat
     1801 ctggtcggta tcgttcgggt cgagcgccac cgcctggtga ccaatgatcg cctgataatc
     1861 ctggaaatcc gtaatgcgga aatcacgtaa cagtgcagtg ccttcgaagc gcgttccctg
     1921 gttgagagtg acgacctggg agccgtcgcg cagctgggtt aaatgtccgg aatcggccac
     1981 caccacagaa ggtcgtgcat taccttttgg tcgaatttgc gcgaggaaca catctttgaa
     2041 atcgctgccg ccaacgcttt cgaggaacag caccgagctg ccattagtcg cttgctggaa
     2101 ttgcccttgc gccagcgccg ccatgccagg gttcgctttc gcttctgcta acacttcatc
     2161 ctgatgacgc gatgaccatg gtcccgccca catcacgtta accgccgcga cgattgccgt
     2221 gaacaccgca aggatcattg ccgctttcac cagcaccgct ttgctcagac cgcaggcatg
     2281 cattaccgta atttcacttt cggtatacag tttgcccagc gtcatcagca gcccgaggaa
     2341 caggcttaat ggcaggataa gctgcgccat ttccggcacg cccaacccga gaagggagag
     2401 caccaggttc gccggaatat cgccgtcaac cgctgcgccg aggatcctca ctaannnnnn
     2461 nnnntaactt ttgacagaag aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt
     2521 tgagcgtctc ccgcaccaga tatcttatga ttatcacttt aaatacgccc gtaaaaactc
     2581 gtcttttgca ggattttagc ttgtttcatg gcttaaacgt catttattct cttgagtcgt
     2641 cgaaatcgtc gctaagataa ttatactcaa cggattcacc tctcagattt tgttctgacg
     2701 tgccaatgcc gtaataacgt taagattaac acgaagtcat cgcaacagcg gacatgagtt
     2761 acgaaagctt gcaattctat ctgtagccac cgccgttgtc tttaagattc aggagcgtag
     2821 tgcatggagt ttagtgtaaa aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc
     2881 gtgggcgtct tcgaaccacg tcgcctttct ccgattgcag aacagctcga taaaatcagc
     2941 gatgggtaca tcagcgccct gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca
     3001 ttgttgctgc accatgtgcc gannnnnnnn nnttccgagc gaattctcct tattggttgc
     3061 ggcaaagaac gtgagctgga tgagcgtcag tacaagcagg ttattcagaa aaccattaat
     3121 acgctgaatg atactggctc aatggaagcg gtctgctttc tgactgaact gcacgttaaa
     3181 ggccgtaaca actactggaa agtgcgtcag gctgtcgaga cggcaaaaga gacgctctac
     3241 agtttcgatc agttgaaaac gaacaagagc gaaccgcgtc gtccgctgcg taaaatggtg
     3301 ttcaacgtgc cgacccgccg tgagctgacc agcggtgaac gcgcgatcca gcatggtctg
     3361 gcgattgccg cagggattaa agccgcgaaa gatctcggca atatgccacc gaatatctgt
     3421 aacgctgctt acctcgcctc tcagg
//
LOCUS       DAWDRC010000441         3423 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_10997_length_3423_cov_1.897565, whole genome shotgun sequence.
ACCESSION   DAWDRC010000441 DAWDRC010000000
VERSION     DAWDRC010000441.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10997_length_3423_cov_1.897565"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1362..1371
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2271..2280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          818 a          969 c          800 g          816 t
ORIGIN      
        1 gtttgaaatg gcgtcccgcc ccgactggtg aatcgtcggg gctgattttt tcttattatt
       61 gcagcagaaa cttctccagg aactggcggg tgcgaggctg ctgaggatca gcaaataacg
      121 cttttgctac cccctgctcg actatccgcc cctggtccat aaaaatcgcc cggtccgcaa
      181 catcccgggc aaagctcatt tcgtgcgtca caatcaccat cgtgcgcttt tcctgcgcca
      241 gctgacgaat ggtgttcagg acttcgccca ccagctctgg atccagcgcc gacgttggct
      301 catcaaacaa aatcacttca ggacgcattg ccagagcacg cgcaatcgca acgcgctgct
      361 gttgaccgcc agacaaacga cgtggataac tggtttcttt accggccagc ccaactttcg
      421 ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg acgatcaccg
      481 gcccttcaat aatgttttcc agcaccgtac gatgcggaaa cagattaaag ttctggaaga
      541 caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt aatgaacgtg
      601 cagtatcaat agtgatatcg ccaacggtga tggttcccgc ttccggttgt tccagcagat
      661 ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataata gccactactt
      721 cgccaggctt cacctcaagg tcgataccat gcagcaccgt ctgaccgtgg aattttttca
      781 ccaggttctt aacttcaatg gcactcattt gggttccctc tcctggcgat taagttgatt
      841 ctcaaaatgg ttctgcaacg tcgataacac agtcgccata atccagtaga tgagcgaagc
      901 cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg cctgacggaa
      961 cagctccggc acctggattg tcgcggccag cgacgtatct tttaccaggc tgataaagct
     1021 gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc gacgcatggt
     1081 ctgccatggc gtcatgccaa tactggctgc agcttcccac tgacctttat caatagaaga
     1141 aatggcggcg cgcagcgttt cggcagcata ggcggcagtg ttcagcgaca gaccaatcat
     1201 tgctgacgga atgggatcta attcgatacc aaactgtggc aatccgtaat agatcataaa
     1261 cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg ccagccagcg
     1321 cacgggccag attggcgaca ggcgcatcag cgcgagaata annnnnnnnn nccgagcagt
     1381 aagccaaaaa acatgccgcc gatactgagt tgcagcgtat acccagcccc tttcagcagg
     1441 aacggcaagg aatcaataac cagttgtata ctttcttgca tgagcgttcc cggagctaaa
     1501 cgtggggatg ataggcgaac agcgcaggcg caccgccggt atgaataaac aaaatcggcc
     1561 cttcatcttt gaagcgtttc tgactgatac cgtcaatcag ccccgccatc gcttttccgg
     1621 tatacacagg atcaagcaga atgccttcca gccgcgccag caatttcact gcttccatgc
     1681 cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc atcccagagt aaaatttccg
     1741 ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg gaggttaacc actttcggca
     1801 attgatcggc aacagaacgc gacacagtca cgccaatcag ttcgctttca ggcatcagct
     1861 gttccagccc aacagccagt ccggcgtgag ttccggcact gcccgacgcg accaccaccg
     1921 acgaaatatt aactgcccct tcacactgtt gcgcaatttc cagcgcactc tccacataac
     1981 ccagcgcgcc caaagcatta gaaccgccaa ccggaatgac atacgggcga aagccttgtg
     2041 cttcgactcg cgtcgccagc tcttccagtt gggtattggg atcggtcagt gcgtcgcaca
     2101 tttcaatctg ggtattgaac agatccagca acaaacgatt gccgttggtt aaatagtttt
     2161 ccgcggttgt accaatagga ttttccagca gcgccacgca atgcagaccg agtttcgcag
     2221 cgactgcggc agtctggcgc acatggttag actggatcgc cccggcagta nnnnnnnnnn
     2281 ttgccgccca ttgccatggg agtgacgtca tcccgtttga tgaaaatttc ccgtccaaga
     2341 taatcagaaa agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccagccgt
     2401 ggaaaacggg ttaaattatg cagtggcata acagcctccg atgtgtgttg ttgtgatttt
     2461 cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc tagtaaaagc gccctttttt
     2521 gtcattatgc tgattatttg gtcacatcag caccaaacca tttttcggaa agggcttgca
     2581 aagtgccatc tttttgcatt tccgcaattg catcattcac cgctttcagc aggtcttcat
     2641 ttcctttacg cagcgccacg ccagactcct gacgggagaa tgcttcaccg gttactgcca
     2701 gcgtatcgtt ggttttcttc accagatcca gcgccgccag acgatcaacg aggatcgcat
     2761 cgatacggcc tacgcgcaga tcctgatatt tggtcgggtc atcatcatag gtacgcacat
     2821 ccacgccctg aacattctga cgcagccact cttcatagtt ggtgcccaga ccgacaccca
     2881 cttttttgcc tttcagatca tcagccgttt taatggtgcc ttcgttgcct tttttcacca
     2941 gcgcctgaat accagaaatg gtgtacgggg tagagaaatc gtattttttc ttgcgctcgt
     3001 cagaaatggt gacctggtta atcaccacat caatgcgttt agagtccagc gacgccagca
     3061 taccgtccca tttggtcggt tttagtgacg cctcaacgcc aagatgtttt gccagctgtt
     3121 gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc cccctgaaaa ctgaacggcg
     3181 gataagttcc ttccagccct accagcagcg tgccgcgctc tttgacttta ttaagcagac
     3241 cttcatctgc aaaactttta acgctcatac ccgcaaccag cgccacggcc atcacaccca
     3301 tcaatgcctg acgtcccaga tgtgctaatt tcatgtttac cccgaatgtt gttatgtctg
     3361 tttgcagtgt agagccattg tttgtaaaca caaaaacaac tccgctacat cttattctta
     3421 ttt
//
LOCUS       DAWDRC010000442         3420 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11002_length_3420_cov_2.032095, whole genome shotgun sequence.
ACCESSION   DAWDRC010000442 DAWDRC010000000
VERSION     DAWDRC010000442.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11002_length_3420_cov_2.032095"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    766..775
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          912 a          768 c          776 g          954 t
ORIGIN      
        1 caataattat cagcatcacg acaaacagtg aatttcgtcc tgattcacga acttgcggag
       61 tcttcttttg ttttttcact ttagcgtttt tattttttgg ggccagcatc ataggaaata
      121 cgcggcacat attaaaaact actgcgagac gctgccagca gattgctgcg aaaaataaac
      181 taaaacaaac gaccgacacc cgccgaatga tgacatcgtc ctgaaagcag aaaaaccaga
      241 ccgtaggtgt aatcattgat atcatccata tgccaggtcc tggacggctg gtaaatcgcc
      301 agaggacaat gatggcaacg atcatcagca ctgtccaggc aaggcatggc agcgaaagtt
      361 gcggattagc aaacagatga ttggctaaag catccaaagg gctaaggttc gctttgatat
      421 gggtaaactg tgcgatgctt tcaattccgc ccgttaatcc agcaaagaga ttgttttttt
      481 cgaattctgg ctgcatatgg taacgaacaa tttgcgcagc ttgttccggc ggcaacatct
      541 gttccagtcc atgtccaatc ttcattgacg ccagcccttc cggccaaacg acgagaatta
      601 agataccttc gccatatcgc ttctctccaa cgggtttcca gtgatatacc ccgtatctct
      661 gcgaggctgc aagtttaatg tttttgcccc ctgttgtcgg cgtaaccaac acggccacct
      721 ggaagtggtt ttgctgctcg agagtctgta cttgttgatt taaagnnnnn nnnnnttctt
      781 ctgtcgttaa tgtcccggtt aagtcagtca ctgaaggcaa tcgttcagcg aatacctgaa
      841 aaccgcatag catcagaagt aagagaaaaa tacgcatatc aattcctttt gttaccagcg
      901 cccggaagcg ccaccgccac cagatgaacc gccaccaccg ctaaaattgt cattgtcgaa
      961 tgaagaggat gacgatgatg aatagtggga ggaagaattg cttcttgtat ggcgtgatgg
     1021 ctggttggca tcacgcatcg aatcccgaac ctttttgccg aagggggtaa aagcgatggc
     1081 aactgcctga aacggtgtgg cgaagcagag taatgttgtg agtactcccc agcttccgct
     1141 gaaacctgct ataggtaaaa caaaggccag tacaatggct gcgaagcaaa ttactcccaa
     1201 cgttttgata tatcctctgg cagaaataaa cgtcagtatt atcgcccaga ctaataaagc
     1261 ccaccaaccg ctaaagggca aagggtgatc ggtcggtgtt atcgttgctg aattattccc
     1321 cgtcaggcga ctttcaatat cgttgatccc cttttgtaaa ccaccagcca ggtcgccttt
     1381 tttaaatgcc ggaataatac tgttgcgaat aattttcccg gactgtgcat ccgtaataat
     1441 accttccagc ccgtaaccga tttcaatacg cacggtatga tcctgccagg cgactaacag
     1501 tagaacgcca tcatcacggt ctttatcacc cggtttccag ctatcgaata cccgcgttgc
     1561 gtactgctcg atggtttcat cgcctgttgt atcaaccact aacacgacca actgtgcgtg
     1621 gtttttttgc gtcagggctt taatctggtt ggtcagggtg tttacttcgc tgtttgttaa
     1681 tgtgccggtt aagtcattca catacctggg tgattcaggt actggaattt gtgtagcaaa
     1741 cacagatgaa cagaagaaag ccataaggaa gataaaattt cgcatagaac gtccccgctt
     1801 aatgagcatt agcattctgg ctaaaatcta ttgttggggc tttacttact gcggtgacat
     1861 catccggcaa ataatttttc tttggtgtgt aatccatgac ctttgcggtt aatacagcgg
     1921 ggaatttgcg gatagtgaca ttatattgtt caatggcttt aatatatctt cctcgcgcaa
     1981 cggcaattct attttcactt ccttcgagct gtaccattaa gttttgatag agctcttgag
     2041 cctttaactg agggtaacgc tcactaataa tcgttaactg cccgagagtg cgagtcacct
     2101 gcgcctgcgc ctgttgccag gcttgtaatt tttgttcatc tcccggagtt ttctgaagat
     2161 cgctactggc gcgattagcc tgactacgtg ccaatgttac ggcttctagt acctcttgtt
     2221 catggctgga gtaaccttta atactagcga caagattagg aataagatcg gtgcgacgtt
     2281 gatattgatt taatacttct gaccaggagg cattaacctg ttcatcatag gtttgaatat
     2341 cgttataacc gcaacctgat aaattgaaaa taaatataaa cgcgatgaat attctaaaca
     2401 tgtttaaatc cctttcattt aatccttgat gatctgagcc aatttttgca tcaaacctgc
     2461 ctgtaacaga atgttaataa gacatgattg ttgtggcagg atctgcaata attaacaatt
     2521 atccacttca cagagaatgc tatgcagtac ccgattaacg agatgttcca gaccctgcaa
     2581 ggtgagggtt actttaccgg cgtccccgcc atttttattc gtttacaggg atgtccggtt
     2641 ggctgtgcct ggtgcgacac caaacacacc tgggaaaagc ttgaggatcg ggaagtctcc
     2701 cttttcagca ttctggcgaa gaccaaagag agtgataagt ggggggcggc gagcagtgaa
     2761 gatttgctgg ctgtcattgg ccgtcaggga tacaccgcgc gtcacgtggt gattacgggt
     2821 ggtgagcctt gcattcatga tttgctgcca ctgactgacc tgctcgaaaa gaacggtttt
     2881 agctgccaga tcgaaaccag tggtactcat gaggtacgct gcacacctaa tacctgggtt
     2941 accgtatcgc caaagctgaa catgcgcggc ggctatgaag tattgtcgca agccctggag
     3001 cgagccaacg aaatcaaaca cccagtagga cgcgtacgtg atatcgaagc actggatgaa
     3061 ttgctggcga cgctgaccga tgataaaccg cgagtcattg cacttcagcc aattagccag
     3121 aaggaagatg ctacgcgttt atgtatcgat acctgcattg cgcgcaactg gcgtttgtcg
     3181 atgcagacac ataaatattt gaatattgcc tgattaaaca tttataagcg ttataaatgg
     3241 gtggaatctg taaagacttc tactcattta tattctttgt cgtctctgaa aaacctccat
     3301 tttgcccatt ctggactaat cattatcatt ctctacaaat tctgtggcgt taatttttcg
     3361 ctggagtgaa aattattgcg gtaaagtttg gtagatttta gtttgaatag agttatttta
//
LOCUS       DAWDRC010000443         3420 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11003_length_3420_cov_1.972065, whole genome shotgun sequence.
ACCESSION   DAWDRC010000443 DAWDRC010000000
VERSION     DAWDRC010000443.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11003_length_3420_cov_1.972065"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          870 a          961 c          874 g          715 t
ORIGIN      
        1 gccgagtata gcggtttccc accggtaaat tgtgctaata gtgttgcgcc aagaccggta
       61 ataatcattg gcaaaatgag ctggtagtta tcggtcattt ccagaaccag aatgatcccc
      121 gttaacggcg cgcgaataga tgccgccagt aacgccccca ttccggcaat agcaaacgtc
      181 cccgcctcaa ggtgatattg cggaaacaac tcaacggcaa ccattccgaa agcggttccc
      241 agcacagtac ccagcgccag catcggggca aaaataccgc ccggcgcgcc ggaagagaag
      301 cagagtaaag tggtaatgac ccgcgcgacg aagataaaca ccagcattcc catgctgaaa
      361 ttccccgcgg tagcgatagg aatcaggtta aaaccgccgc ccgaagttgc tggtgccaca
      421 aaccccagca atccacacag accgccaatc gcaccaccca tcagtaccca tttggtaata
      481 ttgccaccgt gcacacggtg cagcaaatcc tgcatcccca gcacccattt attaaaaata
      541 gggccgaaaa tgccaaaaat aataccgagg atcagataaa gccacagcgt attaagcggc
      601 gcatcagaaa gtttaccgac gtcaatcaac gcaacttcat gattaaaaat ccggtacata
      661 atggtcgaca taatgacgcc aataaatacc gctttaatcg aaattaacgt atagcgaaac
      721 tgcggacgca tctcttcgat aataaacaaa atacccgcca gcggcgcgtt aaaggccgca
      781 gccagccccg cagcagcacc ggttgccagt agcgtatgac gagcttcgtc acctttcagg
      841 cggaaaacat caagtaccat ccgcccgata ttgccgccta tctgcacggt tggcccttcg
      901 cgccccaaca ccatgcctcc gccgagtgtt cccagcccgc caaagaattt caccggcaac
      961 acacgccacc agcgaacggg acgttgatct tccagcgccc cttcaatttc cgggatcccc
     1021 gaaccacctg cttccggcgc gtatttgcgc accaaaaagt agccgaacat cgccagcacc
     1081 gccgaacaga gaaaagcgac ggttaacaga agcggataat tatcagcagt atgtaccagt
     1141 gcccccatac gttggttctg caaccaggcg acacctttgt caaaagcaac cgcagctagc
     1201 ccgacaagcg tgccgacgac tgccgccata aacaaaatgg ctaacggggt tttatcgcgc
     1261 tcaagaagtt ggcgaatcag ttgtctgcgt cgcaggcgcg cggcctgcgg tgtttctaaa
     1321 gagggagtat cagttttcat ccaatgatca cttattggtc atacaaataa gatgacggca
     1381 ttttactcgc acgttccatg aaaatccttt gcaaagcgta atgtttcaaa tgcacaaaac
     1441 ttttatactc taagaaatca ggattgcacc agtacaagca gcctgatgtt tgacgagtat
     1501 ttaacttgtt atgaataaca tagaatagca gccattcact ttttctacga atcaggaacc
     1561 ctccatgagt aagtctgaaa atctttacag cgcagcgctc gagctgatcc ctggcggtgt
     1621 gaactcccct gttcgcgcct ttactggcgt gggcggcact ccactgttta tcgaaaaagc
     1681 ggacggcgct tatctgtacg atgttgatgg caaagcctat atcgattatg tcggttcctg
     1741 ggggccgatg gtgctgggcc ataaccatcc ggcgatccgc aatgccgtga ttgaagccgc
     1801 cgagcgtggt ttaagctttg gtgcaccaac cgaaatggaa gtgaaaatgg cgcaactggt
     1861 gaccgaactg gtcccgacca tggatatggt gcgcatggtg aactccggca ctgaagcgac
     1921 gatgagcgcc atccgcctgg cccgtggttt taccggtcgc gacaaaatta ttaaatttga
     1981 aggttgttac cacggtcacg ctgactgcct gctggtgaaa gccggttctg gcgcactcac
     2041 gttaggccag ccaaattcgc cgggcgttcc ggcagatttc gccaaacata ccttaacctg
     2101 tacttataac gatctggcct ctgtacgcgc cgcgtttgag caatacccgc aagagattgc
     2161 ctgtattatc gtcgagccgg tggcaggcaa tatgaactgc gttccaccgc tgccagagtt
     2221 cctgccaggt ctgcgcgcgc tgtgcgacga atttggcgcg ttgctgatca tcgatgaagt
     2281 gatgaccggt ttccgcgtag cgctggctgg cgcacaggat tattacggtg tggaaccgga
     2341 tctcacctgc ctcggtaaaa tcatcggcgg tggaatgccg gtaggcgcat tcggtggtcg
     2401 tcgtgatgta atggatgcgc tggccccgac cggtccggtc tatcaggcgg gtacgctttc
     2461 cggtaacccg attgcgatgg ctgctggttt cgcctgtctg aatgaagtcg cgcagccggg
     2521 cgttcacgaa acgctggatg agctgacaac acgtctggca gaaggtctgc tggaagcggc
     2581 agaagaagcc ggaattccgc tggtggtaaa ccacgttggc ggcatgttcg gtattttctt
     2641 taccgacgcc gagtccgtga cgtgctatca ggatgtgatg gcctgtgacg tggaacgctt
     2701 taagcgtttc ttccatatga tgctggacga aggtgtttac ctggcaccgt cagcgtttga
     2761 agcgggcttt atgtccgtgg cgcatagcat ggaagatatc aataacacca tcgatgctgc
     2821 acgtcgggtg tttgcgaagt tgtgatgatt ttgcctcgcc caccgggcga ggcattcaat
     2881 taacggctct gctttctcaa caaatagata aaatatggcg cgccgataaa ggtcgacagc
     2941 agccccgcgg ggatctggaa tggaaacagc accatccgcc cacaccagtc agcgaacacc
     3001 agcagtaaac cacccaccag cgccgaaatt acgatgtgtg gcatcgtccg tcgaaagccc
     3061 atcatccgcg caatatgcgg tgccattaaa ccaacaaaac tcaacggtcc aatggtcatt
     3121 gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg caattcgcgt cggcgtcagc
     3181 gccatcccca cggctcgtgc ggtatcacca cccagcggta aaatggtcag ccagcggcgg
     3241 cacagcgggg taatcgccag caaaatcacc atcacaattc cagtacgcca gacctgcgca
     3301 tcggtcgcgt tgtaggtcga accggaaatc caggtcagaa cttgcgccat tcgcgggtca
     3361 ccacttgcct gcaacatcat caaaagcatg gtgaacgggg tactaaacga catccccgcc
//
LOCUS       DAWDRC010000444         3419 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11005_length_3419_cov_2.291023, whole genome shotgun sequence.
ACCESSION   DAWDRC010000444 DAWDRC010000000
VERSION     DAWDRC010000444.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11005_length_3419_cov_2.291023"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1307..1316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          752 a          940 c          892 g          825 t
ORIGIN      
        1 ctctttcgct ttctcaaccg ccatttcgat ggtggtaacg gtggcgttcg ccggttgttt
       61 cagtttcaga cgctcaaccg catgctggaa gcgttcacgg tcttccgcac ggtcgatggc
      121 atccgggctg gtaccgataa ccggtacgcc agcagcttcc agcgcgcgcg ccagtttcag
      181 cggggtctga ccgccgtact ggacgataac gcctttcggc ttctcaatac gcacaatttc
      241 cagcacgtct tccagagtta ccggctcgaa gtagagacgg tcagaggtgt cgtagtcggt
      301 ggagacggtt tccgggttac agttaaccat aatggtttca taaccatctt cgcgcagcgc
      361 cagcgaggcg tgtacacagc agtagtcgaa ttcgataccc tgaccgatac ggttcgggcc
      421 gccgccgagc accatgattt tttcacgatc ggtggacgga ttcgcttcgc actcttcttc
      481 ataagtggag tacatgtaag cggtgtcggt ggcgaactct gccgcacaag tatccacgcg
      541 cttgtaaacc gggtgcagat catactggtc gcgcaacttg cggatttccg cttcgcgcac
      601 gcccgccagt ttagccagac gcgcatcggc aaagcctttg cgtttcaact ggcgcaggaa
      661 ttcagcgtgc aggccagtga tgcccacttc tgcgactttc tcttccagac gcaccagctc
      721 ttcaatctgt accaggaacc agcggtcaat gttggtcaaa ttaaacacgc cgtctacaga
      781 caggcccgcg cggaaggcat cagcgatata ccagatacgc tctgcgccag catctttcag
      841 ttcgcggcgg attttagtca gcgcttccgg gtcgtcgaga ctcactttcg gatcgaagcc
      901 agttgcgccc acttccaggc cgcgcagcgc tttttgtagg gattcctgct gcgtgcgacc
      961 aatcgccatc acttcgccaa ccgatttcat ctgagtggtc agacggtcgt tagcaccggc
     1021 gaatttctcg aagttgaagc gaggaatttt agtaaccaca tagtcgatgg acggctcgaa
     1081 ggaggccgga gtacgcccac cggtgatgtc gttcatcagc tcgtcgaggg tgtaacccac
     1141 cgccagtttc gccgccactt tggcaatcgg gaaaccagtg gctttcgacg ccagcgcaga
     1201 ggagcgagac acgcgtgggt tcatttcgat aacaatcagg cgaccgtttt tcgggttcac
     1261 cgcaaactgg acgttggaac caccggttta aacgccgatt tcacgcnnnn nnnnnncgcg
     1321 acagtgatgg agtcaccggt gtggatgccc atcgcatcaa agttttcgat agagcagacg
     1381 atgatgcagt tgtcgttttt atcacgcacc acttccatct cgtactcttt ccagccgatc
     1441 agcgactcat caatcagcag ctctttagtc ggagaaagat ccagaccgcg ggcgcaaatt
     1501 tcttcaaatt cttcacggtt ataagcgata ccgccaccgc taccgcccat ggtaaaggat
     1561 gggcgaataa tgcacgggaa gccaacttca gcggcaaccg ccagcgcttc ttccattgaa
     1621 tgagcgatac cggaacgcgc agtttccaga ccgattttct tcatcgctac gtcgaaacgg
     1681 cggcggtctt cggctttatc aatcgcatcg gcagtggcac caatcatggt gacgccgaac
     1741 tcttccagca cgccctgacg ctcaagctcc agcgcacagt tcagcgccgt ctgccccccc
     1801 atggttggca gcaccgcgtc cggacgctct ttttcaataa tcttgcgtac cacttcccag
     1861 tgaatcggct cgatgtaggt ggcatcagcc atttccgggt cggtcatgat ggtcgccggg
     1921 ttggagttca ccagaatgac gcggtaaccc tcttcacgca gggctttaca cgcttgcgcg
     1981 ccagagtagt caaactcaca cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg
     2041 atacttttta tatctgtacg ttttggcatg gctcttttac tcctgattac ttagcggttt
     2101 tacggtactg ctcaattaac gcgataaagt ggtcaaacaa cggcgcggcg tcgtgtggac
     2161 cagggctggc ttccgggtgt ccctggaagc tgaacgccgg tttatcggta cgatgaatgc
     2221 cctgtaacgt accgtcgaac agggatttat gtgtgacacg caggtttgca ggtaatgttg
     2281 cttcatccac cgcaaaaccg tggttctggg cggtgatcat caccacattt ttctcaacat
     2341 ctttaaccgg atggttgccg ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc
     2401 tcgccagcgc cagcagctga tgaccgaggc agatgccgaa taccggaata tcagtttcga
     2461 ggaatttctg gatagcggta atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg
     2521 agaggaagat gccgtccgga ttcattttca gcacatcttc cgcagaagtt tgcgccggaa
     2581 cgatggtcag gcgacagcct ctatccacca gcatacgcag gatgttgcgc ttggcaccga
     2641 aatcgtaggc cacgacgtgg tacggcagct cgtcttcttt tttcgcttct ggcaggccgc
     2701 cggtcagtgt ccagctccct tgtgtccagc tatagggttc tgcggtggtt acttcttttg
     2761 ccagatccat gccgttcaga cctgggaacg cgcgggcttt ttctaatgcc agcgccgcat
     2821 ccgggttatc gcctgcgata atgcagccat tctgagcacc tttctcacgc agtaaacgcg
     2881 tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag
     2941 agaggtcttc ggtattacgg aagttgctgg caatcagcgg caggtcgcga atcaccagac
     3001 cttgggcatg aacctgagaa gattcttcat cggcgtcatt agtgccgaca ttgccaatat
     3061 ggggataagt aagagtaacg atttgacgag aataggaagg atcagtgagg atttcttgat
     3121 aaccggtcat tgaagtattg aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg
     3181 cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag cgctgactta atcaaaacac
     3241 cctccagaga atattcactc actttatttg catattaatt cacaatgatg acgtaaatca
     3301 atgcaaatct gcttaccgac gaatttctgg caaacggcgg cattctggag atatgggagc
     3361 taaaagtcaa cttaatggcg ggatttttta tattattttg tggctcatgc atgttttaa
//
LOCUS       DAWDRC010000445         3417 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11011_length_3417_cov_2.070196, whole genome shotgun sequence.
ACCESSION   DAWDRC010000445 DAWDRC010000000
VERSION     DAWDRC010000445.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11011_length_3417_cov_2.070196"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    458..467
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1815..1824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          887 a          932 c          807 g          771 t
ORIGIN      
        1 ccgggttttc tgtaccgtca acaaagccgc tcaggtcacg ctcttcaacc caacggaagc
       61 cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga
      121 cagagaagtt cacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg
      181 tcggtgcaag acctttaccg tagcccggaa aatctttcag ctcttcagcc ccaacgccgc
      241 cgctcagagt gcgccaggta ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg
      301 ggaatttcgc ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc gcacgcaggg
      361 cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca
      421 aaatgccact ctgaacctga gacattgttc ctcctgannn nnnnnnnacg caatccgtgg
      481 cattgttggg cgctatttta cgtctttttt cgcggcggcg gtttgttcag acgcaaatta
      541 acgccgccag ataattttac tgactttcca gtttttcagg gtatcgtccg aaggcattaa
      601 cccttcagga ccactccatt ccccgctgaa ctggtagcta atatgttgac tgccttcagc
      661 tttacattcg acggcacgat tatcatcact gtcggccttt tggcaattgc caaatgcttt
      721 gctgtaaagg tcactaaacg gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct
      781 atccagcaca tcaatgcggc tgatcgtgcc ttgatcaccg ttaatcacca tcgccacgtt
      841 gtcgcctttc atcacttcaa aaaagcgcac cacgttgccg ttcgcggttt tcattccgct
      901 gcgcaggcga taatcgccat caagcgcatc agcaatggct tgttcttgca gtggtgtgga
      961 cgccgttaat tcacccacgc cctgctcgct cactttggtg gatgatccaa accagttcca
     1021 cgggttagcg gccgaccagt taactgacga catcgtggaa cagccggtca gcatcaacgg
     1081 catagcacat aacattaaac gcagcgattt catgtcactt cctttggtta ttcaataacg
     1141 ttgcttggag tgcaaattca ccaaaaagtg ccgttattct ttttcttcat taaaacaagc
     1201 gcgtaaacgt cgattggtca gcagccagat taacgccacg atatccgcca ctaccagcgc
     1261 cagaccgata ccgctaacgg attcaccgtt cagccacagc cacggttgcc aacaaagtaa
     1321 aaccacttgc gccaacagca acagaaaata gagcacatgc cagatacggg gaaatgaagc
     1381 ccgccgacca ctcagcaaaa atgccagcac cgccggaata ccgggaatca accccagcca
     1441 gaaattatcg tgatcgggat aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc
     1501 tccggcaatg acaaacagca cccaggttcg cgcctgaagc aatagcacaa gccagaagag
     1561 caagggtaaa cgcaggcgac cgtgcgcatc ataatggaca ggatgaaact cagtactctt
     1621 catcttcaat caaacgctta cccagactca acacgtcggc gtgttcatat cccaggcgtt
     1681 catacattcc gagcaccatg tcgttatctt ccggcacgtt gatctgaatt ttcggacagc
     1741 cgcgagcaat cagctttttc tccagccgat taagcaacgc attagcaatc ccacgcccac
     1801 gaaactcggg atgcnnnnnn nnnnatgcac gccaagataa taagcagacc cgcgatgccc
     1861 gtcataaccg cccatcaccg ttccgaccac ttcaccgttc acctcagcga ccagaaacaa
     1921 actgacgtca tggttcatct tacgctcgat gtccatttcc ggatcgttcc acggacgcag
     1981 caagtcgcaa cgctcccaaa gggtgatgac ctcttcgaaa tcttcctggc gaaatacgcg
     2041 tatctccatg gtattcgtta cctttttgcg ggttaaaagg ctgattatgg cgtgaacggt
     2101 cgaattagcc aatatctgac gaaaatcggt tgaaaaagtg gcataatggg gagttgtcaa
     2161 ctattgaaat gaaaagtaaa acaattctca acagcaaacc gtcgtaacgg attacgcgat
     2221 acgatataac atctggaact ttattattac aactcaggcc gtatgagcac ttttaaacca
     2281 ctaaaaacac tcacttcgcg ccgccaggtg ctgaaagccg gtttggctgc cctgacgtta
     2341 tcaggaatgt cgcaagccat cgccaaagaa gaaccgttaa aaaccagtaa cggacacagc
     2401 aagccgaaag caaaaaaatc tggtggcaaa cgtgtcgttg ttctcgatcc aggccacggc
     2461 gggattgata ccggagcgat cggacgcaac ggttcgaaag aaaaacatgt ggtgctggca
     2521 attgccaaga atgtccgttc catattgcgt aatcatggca ttgatgcgcg tttaacgcgt
     2581 tctggcgata cgtttatccc actgtacgat cgcgtggaaa tcgcccataa acacggcgca
     2641 gacctgttta tgtcaattca cgccgacggt tttaccaacc cgaaagctgc cggtgcttcg
     2701 gtatttgccc tctctaaccg tggggcaagt agcgcaatgg cgaaatacct gtctgaacgc
     2761 gaaaaccgcg ccgatgaagt tgccggtaaa aaagcgactg acaaggatca cctattgcag
     2821 caagtgctgt ttgatctggt gcaaaccgat actattaaaa acagcctgac gctcggctcg
     2881 catattctga aaaagattaa gccggtgcat aaactgcaca gccgcaatac cgaacaagcc
     2941 gcgtttgtgg tgttgaaatc gccgtcggtt ccgtcggtgc tggtggaaac ctcgtttatc
     3001 accaacccgg aagaagagcg gctgttaggc actgcagcgt ttcgccagaa aatcgccaca
     3061 gcgattgccg aaggcgtgat cagttatttc cactggtttg acaaccagaa agcacactcg
     3121 agaaagcggt aagtgatgaa acccgacgca caccaggtta accttcagga tacgatttgt
     3181 cagcagttga gcgccgtcga tggcgcagaa tttgtcgaag atagctggca gcgcgaagct
     3241 ggcggcggcg ggcgtagtcg ggttttgcgt aatggtggtg ttttcgaaca ggcaggcgtc
     3301 aacttttcac atgtccacgg tgaggcgatg cctgcttccg ccaccgctca tcgtccggaa
     3361 cttgccgggc gcagtttggg ggcgatgggc gtttcactgg tagtgcatcc gcataat
//
LOCUS       DAWDRC010000446         3412 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11024_length_3412_cov_2.039619, whole genome shotgun sequence.
ACCESSION   DAWDRC010000446 DAWDRC010000000
VERSION     DAWDRC010000446.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11024_length_3412_cov_2.039619"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    621..630
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1412..1421
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          792 a          781 c          875 g          944 t
ORIGIN      
        1 atgcgcgggc atccggtttt tatcgctcaa cacgccaccg cgacctgttg tcgcggttgt
       61 ctggctaagt ggcataacat tcctcagggg gaagcgttaa gtgaggagca acaacgttat
      121 attgtgacgg taatctacca ctggctggtt atacagatga atcagccatg ataattacgg
      181 aaatcgataa aatggcattc attaggtatc tttagttgcc gccgttgtca gcgaacatgc
      241 tatcctttct aagaatttac ttattgcgtt gagataattg ccacccgcaa ttgtatggac
      301 agataattaa tgcatgtaca gcccatttca actttccgtt tgttccagga aggtcatctg
      361 ctacgtaata gcatcgctat ttttgtgcta accacgctgt tctattttat tggtgcagag
      421 ttacggctgg ttcacgaact ttctcttttc tggccgctga atggcgtaat ggcgggggtg
      481 tttgcccgct atgtctggct taatcgactg cattactatg cgatcagtta tgtggcgatg
      541 ctggtttatg atgccataac caccgaatgg gggctggttt cactggctat caatttctcc
      601 aatatgatgt ttattgttac nnnnnnnnnn tgtctgcttg ccgcattatt atgcgctatt
      661 gtcggggcga ttggttcggt cagtattgat agtctggatt tctggccttt gcttgccgac
      721 tggtttagtg agcaattctc aacgggcgtg ttgatcgtgc cttgtatgct gacgttggca
      781 attcctggag tgctgccgcg ctttaaagca gagcagatga tgcctgctat cgcgcttatt
      841 gtgtcggtta ttgcctcggt agtcattggt ggagcgggga gtctggcgtt tccgctccct
      901 gcattaatct ggtgtgcagt gcgctatacg ccgcaggtaa catgtctgtt gacctttgtc
      961 accggtgcgg tggaagtcgt actggtggca aattcggtga ttgatatctc ggtcggttcg
     1021 ccgttctcta ttccgcaaat gttctccgca cgtctcggta ttgccacgat ggcgatatgc
     1081 ccaattatgg tttcctttag cgtggcagcg atccattcgc taatgaagca agttgcgctg
     1141 cgagccgact ttgattttct gactcaggtt tactcacggt ctggtcttta tgaggcgctg
     1201 aaaagtccat cgctgaaaca gacacaacat ctgacggtca tgctgcttga tatcgactat
     1261 ttcaaaagca ttaacgataa ctatggacat gaatgtggcg ataaagtgtt aagcgtgttt
     1321 gcccagcata ttcagaagat tgtcggtgat aaggggctgg tggcgcgaat gggcggcgaa
     1381 gaatttgctg ttgcagtgcc gtcggtgaat cnnnnnnnnn nttcaaccgt tcacctggca
     1441 acaaaaaacg ctttatctca cggtaagtat tggcgtcggt agtggttgcg catcgtaccg
     1501 aacgctgacc gatgacttta ataaattgat ggtcgaagcc gatacgtgtc tgtatcgctc
     1561 gaagaaagat gggcgcaacc gtaccagcac tatgcgttac ggtgaggaag ttgtctgaca
     1621 tctttattta ctcagagcga attaaatgca atatgttcta ccactttatg atccaagaaa
     1681 aggcctgttt cctcgattaa accatgatcc gcgccacact tttctcataa aactgctcat
     1741 taatcatcag gagttgatca aaacaggggt tcggcgtatt aaagtttgct aacctgtcgt
     1801 accaattcaa acaggttgta tgactaatca gaagggaacc cattgtgaaa acactaaatc
     1861 gtcgcgattt tcccggtgca cagtatccag aacgtatcat tcagtttggt gaaggtaact
     1921 tcctgcgcgc ctttgttgac tggcaaatcg atctcctgaa tgagcacacc gatctgaatt
     1981 ctggcgtggt cgttgttcgt ccgattgaaa cttcattccc gccgtcactg agcacgcagg
     2041 atggtcttta taccaccatt atccgtggcc tgaatgagaa gggtgaagcg gtcagcgacg
     2101 cgcgcctgat tcgctcggtt aatcgtgaaa tcagcgtcta cagtgaatac gatgaattcc
     2161 tgaaactggc tcacaatccg gaaatgcgct ttgttttctc taacaccacc gaagcgggta
     2221 tcagctacca tgcgggcgat aaattcgatg acgcgccagc ggtaagttat ccggcaaaac
     2281 tgacccgtct gctgttcgaa cgttttagcc atttcaacgg tgcgctggat aaaggttgga
     2341 tcatcatccc gtgtgagttg attgactata atggcgacgc gttgcgtgaa ctggtgctgc
     2401 gctatgcgca agagtgggca ctgccagaag catttattca atggctggat caggctaaca
     2461 gcttctgttc tacgctggtg gaccgtatcg ttaccggtta tccgcgtgat gaagtggcta
     2521 aactggaaga agaactgggc taccacgatg gtttcctcga caccgctgaa cacttttacc
     2581 tgtttgtgat tcaggggccg aaatccttag cgactgaact tcgtctggac aaatatccgc
     2641 tcaacgtgct gatcgttgac gatattaaac cgtataaaga acgcaaagtg gcaatcctca
     2701 acggtgcgca caccgcgctg gtgccggtgg cgttccaggc agggctggat accgtaggtg
     2761 aagcgatgaa cgatgctgaa atttgcgcat tcgttgaaaa agccatctac gaagaaatta
     2821 ttccggtact ggatttgcca cgcgatgagc tggagtcttt cgccagtgca gtcaccggac
     2881 gtttccgtaa cccgtacatc aagcatcaac tgctgtctat cgcgctcaac ggtatgacca
     2941 agttccgcac acgcatcctg ccgcagctgc tggcagggca gaaggcaaac ggcacacttc
     3001 cggcgcgcct tactttcgca ttagcggcat tgattgcgtt ttatcgcggt gagcgtaatg
     3061 gagaaacata tccggtacag gacgatgcac actggctgga acgttatcag caactctgga
     3121 gccagtatcg tgaccgtgtt atcgggaccc aggaactggt agcgattgtg ttggcagaaa
     3181 aagaccactg ggagcaggac ctgacgcaag tgcctggtct ggtcgagcag gtggctaatg
     3241 acctggatgc gattctcgaa aaagggatgc gcgaagctgt acgcccgttg tgctaatact
     3301 ctgaacccgg tttcgaccgg gtttcttatt tcgctatatt agttacaaca ttctatatgc
     3361 ctttatcatt aacaggtgcc acatgcagca cctgttaatg ttgtaatctt gt
//
LOCUS       DAWDRC010000447         3400 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11052_length_3400_cov_2.356353, whole genome shotgun sequence.
ACCESSION   DAWDRC010000447 DAWDRC010000000
VERSION     DAWDRC010000447.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11052_length_3400_cov_2.356353"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          880 a          929 c          837 g          754 t
ORIGIN      
        1 agtcatcgcc aaaccgttca cgtaaacgct tatccaccag attcatcccc agcccactgg
       61 cattggttac cggttgatac aaacctgcat tgtcttcgat ctccagcatc aaatgttgcc
      121 cctcacgtcg ggcgctgatt gccactcgcc ctgtatccag cagttgtgat gtcccatgtt
      181 taatggcgtt ttccactatc ggttgcaggg taaacgcggg caattgctgc tgggataatt
      241 cttgcggaat agcaatgttg acctgcaacc gcgactggaa gcgcgccttt tcaatttgca
      301 gataagcgtt cacatgttca atttcgtcgg cgagagtaac aaactccgaa ggccgcttta
      361 agtttttgcg gaaaaaagtg gaaagatact gcaccagctg gctggcctgt tcgctgtcgc
      421 ggcggatcac cgctttaatg gtgttaagcg cattaaacaa aaaatgggga ttcacctggg
      481 cgtgaagcag tttgatctct gactgggtga gcatcgcttt ttgccgctca tattgcccgg
      541 cgaggatctg cgccgaaagc agttgcgcaa tcccctcgcc cagcgtgcgg ttgattgaac
      601 tgaataaacg gtttttggct tcatacaatt tgatggtacc catcactcgc tgattttcac
      661 cacgcaacgg aatgaccagc gtcgacccca gtttgcattg cggatgcaaa gagcaacggt
      721 aaggcacttc gttgccatca gcgtagacca cttcaccggt ttcaatcgct tttaaggtat
      781 aagttgaaga aatcggtttg ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca
      841 gcaatttctc tcgatcggta atcgcgactg caccaatatc cagctcctga tacagcacct
      901 gtgccacttt catgctgttc tcttcgttaa acccctggcg caaaatgcct tccgtcgagg
      961 ccgccacttt cagcgcagtg gcagaaaaag ccgaagtgta tttttcaaac atcgcgcgtt
     1021 tatcgagcaa tatacgcata aacagtgccg cgccgacggt attggtgacc atcattggcg
     1081 cagcaatatt actcaccaga cgcaccgcat cttcataagg tcgggcgatc gccaggatga
     1141 tcaacatttg caccatttca gcgacgaacg tgacggcacc ggcggtaata gggttaaaga
     1201 ctttatcagt gcgcccgcga cggatcagga tgctgtgtac caggccgccg agcaatcctt
     1261 caacgatggt cgagatcatg caacttagcg cggtcatgcc ccccatcgaa tatcgatgta
     1321 acccgccggt cagaccaacc agcccaccga cgaccggacc gccgagtaag ccgcccatta
     1381 ccgcgcctat cgcacgggta ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc
     1441 ccatgatgca gaagatggaa aagacgatgt agcagagaaa tttatgcggc agacgaaccg
     1501 tgacctgcat taacggtatg aataatggcg ttttactcat taaccacgca atgactaaaa
     1561 aaacgcacat ctgctgaagc agcagcaaca ccagattaaa atcgtacata cccgcaaacc
     1621 acacttccct ttaaaatgcg taacatacat tgcttgcgtt taactttctt tgaactcttg
     1681 cagaaaaatg agaattcgtg agtacgatca ctcaaaatcg cctggcaaaa ataaaatcac
     1741 cctatagatg cacaaaaaac gggcaaaact acctggttcg caaaactgcg tctaaagtta
     1801 aaccgggacc tcgcgagcaa gggtgagacg atggcgcttt acacaattgg tgaagtggcg
     1861 ttgctttgtg atattaaccc tgtcacgtta cgcgcgtggc agaggcgtta cggattgctg
     1921 aaaccgcaac ggacagacgg cggtcatcgg ctgttcaacg atgccgatat tgaccggatc
     1981 cgcgagatca aacgctggat cgaaaacggc gtgcaggtca gcaaagtaaa aatgctgctc
     2041 agtaatgaaa atgttgatgt gcagaacggc tggcgcgatc agcaagaaac attactgact
     2101 tacctgcaaa gcggcaatct gcatagcctg cgaacgtgga tcaaagagcg cggtcaggat
     2161 taccccgccc agacactcac cacacatctg tttattcctc tgcgccgacg gcttcagtgc
     2221 caacaaccga ctctccaggc gctgctggcg atcctcgacg gcgtactgat caactacatc
     2281 tctatttgcc tggcttcggc gcgcaaaaaa cagggtaaag atgcactggt ggttggctgg
     2341 aatattcagg ataccactcg tctgtggctg gagggctgga ttgccagtca acaaggatgg
     2401 cgcattgatg tcctcgccca ctcgctcaat caactacgcc ctgaactgtt cgaaggccgt
     2461 acattgctgg tatggtgcgg tgataatcga acctccgccc aacagcagca actcaccagt
     2521 tggcaggaac aaggccatga tattttccca ctcggcattt aatgattcgt taacaaatgc
     2581 gctttactgt acaatccttt cgttaacata aggagtgcat tatgcgcata gctaaaattg
     2641 gggtcatcgc cctgttcctg tttatggcgt taggcggaat tggtggcgtc atgctcgcag
     2701 gttatacctt tattttgcgt gctggctgaa cgcctgcacc agcctttcaa acaggcggtc
     2761 tgcgatgatc gccgccagtg ccaccagtaa cgctccctgg atcacatacg cggtattaaa
     2821 tccgctaagt ccgatgatga tgggcgtacc cagcgtgctg gcccctaccg ttgaggcgat
     2881 cgtcgccgta ccaatgttga taatcaccga agttcgcacg cccgccagaa tcaccggagc
     2941 cgccagcggt agctcgacct tacgcagtcg ctgaccacga ctcattccca tacctttcgc
     3001 aacttctgtc acgctggcat caatcgctcc cagcccggca agtgtcgcct gcaggacggg
     3061 cagcacgccg taaaggatca aggcgataat cgctggtttc agaccaaagc cgatcaccgg
     3121 aacggcaatc gccagcactg caacgggcgg aaaagtctgt ccaacggcgg caatagtttc
     3181 caccagtggg cgaaattccg caccccacgg gcgagtgaca gcaattccgg caccagtacc
     3241 aatgatcacc gcaaacaaac tcgaaattcc caccagccag aaatgagcca gtgccagagc
     3301 tgcaaagctt tcctgctgat aaacgggtcg tggcagttgt gggaacaagg cagcaaacag
     3361 cggctggctg taaggcagcc agaaaatcag cgccacaaac
//
LOCUS       DAWDRC010000448         3387 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11099_length_3387_cov_2.899760, whole genome shotgun sequence.
ACCESSION   DAWDRC010000448 DAWDRC010000000
VERSION     DAWDRC010000448.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11099_length_3387_cov_2.899760"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1124..1133
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          909 a          834 c          817 g          817 t
ORIGIN      
        1 aaaaaattca atttcagtat gaatgttgtt cccttcctcg tagttcatcc tttgcacgct
       61 gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc acaaataaac
      121 aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc ttatgcacat
      181 aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg cagccctggg
      241 agtaaatgcg cccccaatca ctgttgaggt acatatcagt aaaggtctac caggcttaac
      301 gatggtgggc ttaccagaaa caacggtaaa agaagctcgc gatcgcgtgc gcagcgccat
      361 tatcaatagc ggatatgaat atccggcgaa aaaaatcacc atcaaccttg ctccagccga
      421 tctgccaaaa gaagggggac gatatgattt gcctatcgct attgcgttac tggcggcctc
      481 agaacagctt acagccaata agttagatga atatgaatta gtcggagaac tggcgcttac
      541 aggcgctctg cgtggcgttc ccggcgcaat ctccagtgca actgaagcta ttaagtcggg
      601 cagaaaaatt atcgtcgcga aagataacga agatgaagtg gggctaatta acggtgaagg
      661 atgcctgata gccgatcatc tgcaggctgt ctgcgcgttt ctggaaggta agcacgctct
      721 cgaacacccg aaaccaacta atgcagtatc ccgggcgcta caacatgatc tcagtgatgt
      781 tgtcggtcag gagcaaggaa agcgaggact ggaaattacc gccgctggcg ggcacaacct
      841 tttactgatt ggaccgccgg gaacaggtaa aacaatgctc gccagccgta ttaatggcct
      901 tttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa gtctggtaaa
      961 tgctgaatca gtacaaaaac aatggcggca gcgcccgttc cgctcacctc atcacagtgc
     1021 gtcgttaact gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa tttcgctggc
     1081 gcataacggc gtgctttttc ttgatgagct acctgaattt gaannnnnnn nnngcgagag
     1141 ccgattgaat ccgggcagat ccatctttca cgcacacgag caaaaataac ctatccagcc
     1201 cgtttccagc ttgtcgcggc gatgaatccc agccctaccg gacattatca gggaaaccat
     1261 aaccgctgca caccagaaca gacattacgt tatctcaacc ggctctcggg gccctttctc
     1321 gaccgcttcg atctctcact ggagatccca ttaccgcccc ccggcatttt gagtaaaacg
     1381 gtagtgccgg gagaaaacag caccaccgtt aaacaacgtg taatggccgc cagagagcgc
     1441 caatttaagc ggcagaataa actgaacgcc tggctggata atccggaaat acgccaattc
     1501 tgcaagcttg aaagcgaaga tgcgcagtgg ctggaagaaa cgctgatcca tctggggtta
     1561 tcgattcgtg cctggcagcg gttattgaaa gttgcacgaa ccattgctga tattgatcag
     1621 tctgacatta tcacacgtca gcatttgcag gaggcagtta gctatcgtgc gattgaccgt
     1681 ttgctcatcc atctgcaaaa actactgaca taaaaaaagg gcatttcgcc ctttttatta
     1741 atcgtcagaa tcggtgtagt cttcagcacc ttcaacctgc ggtttaccgc cggaaagggt
     1801 gtgaaaacgt tttggacgct tgatacgcgt catatacttg gaccacacgc gttctgcttc
     1861 tgtcactggt tcacgttcgc cacggcatac tgctacgaag agtttctctt cctcggtaac
     1921 cggctcgcgt ttgccaagat ccaactcatt gaaggcataa ccatgacgct caagcagttg
     1981 tgcctctttg atggtgaagt caccatgacg agagaatcca cgtggataat gtttattgtc
     2041 gaaatatcga ttagtcgtcg taaagctttc cgccatcctg cacgctccta attctttgac
     2101 cgagctagtt atggcgcgga gtattagtta cgcttgacag agtgtaaaac aaaacattta
     2161 aatcataacg acaaataatt ttgcggagag cactgtggat acggaattgt taaaaacttt
     2221 cctggaagtt agccgaacgc gtcactttgg tcgagcggct gaatcgctct atctgaccca
     2281 atcagccgtg agctttcgaa tcagacaact ggaaaatcaa ctgggtgtga atcttttcac
     2341 ccgccacaga aacaatatcc gtttaaccgc tgctggtgaa aaactactgc cttatgcaga
     2401 aacgctcatg agcacctggc aggccgcccg taaggaggtg gcgcatacct cacgacataa
     2461 cgagttttct atcggtgcca gcgcctcgtt gtgggaatgt atgcttaatc agtggctggg
     2521 acgcttgtat caaaatcagg atgtccatac aggcttacag ttcgaagcgc gaattgccca
     2581 acggcagtct ctggtaaaac agttgcatga acgccagctt gatcttctta ttacaactga
     2641 agcccccaaa atggacgaat tttgtagtca gttgctgggg tatttcactt tagcgcttta
     2701 taccagtgcc ccttctaaac taaagggaga tcttaactat ctgcgactag agtgggggcc
     2761 agattttcaa cagcatgagg caggtttgat cggtgctgac gaagttccca ttctgacaac
     2821 cagttctgct gaactggcac agcaacagat tgcgatgctt aatggttgca cctggctacc
     2881 cgtcagttgg gcacgtaaaa aaggcggcct gcataccgtt gtcgatagca caacactttc
     2941 acggccgctt tatgccatat ggctacaaaa tagcgataaa aatgcgttga ttcgcgatct
     3001 attgaaaatt aacgtgctgg atgaagtgta ttaatctgaa tggctggcaa ggatgccggt
     3061 agaaggagtt acttcggaga gggttatttc agataaaaaa aatccttagc tttcgctaag
     3121 gatgatttct ggcaggggcg gagagactcg aactcccaac acccggtttt ggagaccggt
     3181 gctctaccaa ttgaactacg cccctaatta gggtggcgga acggacggga ctcgaacccg
     3241 cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc accgaattct
     3301 tttacaacca ccggttttat gaccggctta ctgctaaatt tgatgcctgg cagttcccta
     3361 ctctcgcatg gggagacccc acactac
//
LOCUS       DAWDRC010000449         3363 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11178_length_3363_cov_1.929565, whole genome shotgun sequence.
ACCESSION   DAWDRC010000449 DAWDRC010000000
VERSION     DAWDRC010000449.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11178_length_3363_cov_1.929565"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          855 a          831 c          796 g          881 t
ORIGIN      
        1 atagcccttc gctctccggc aacttgcagg ctatgatagt tctccagcca caggagaaca
       61 ttaatgaaac cgcaggttta ccacgtcgat gcttttacct ctcaaccgtt tcgcggcaat
      121 tctgccggtg ttgttttccc cgccgataat ctcagtgaag cgcaaatgca gcttatcgcc
      181 cgcgagttag gtcattcgga aactgctttt ctgctgcaca gcgacgacag cgatgtgcgc
      241 atccgctact ttacgccaac ggttgaagcg ccaatttgcg gtcacgcgac ggtggctgcg
      301 cactatgtac gtgcaaaggt attaggtttg ggaaattgca cggtctggca aacgtcgctg
      361 gcaggacaac accgcgtgac tatcgaaaag cataacgatg attatcgtat ttcgctggaa
      421 caaggtacgc cgggctttga gccaccgctg gaaggtgaaa cacgtgcagc gattatcaac
      481 gcgcttcatc tcactgagga cgatattctg ccaggcttgc caattcaggt ggcaacaaca
      541 ggacactcaa aagtgatgat tccactgaag tcggaagtgg atatcgacgc cctttcgcct
      601 gaccttgctg cgctgaccgc aatcagcaag cagattggct gcaatggttt cttcccgttc
      661 cagatccgcc caggcaaaag cgaaaccgac ggtcgcatgt tctcgcctgc gattggtatt
      721 gtggaagatc cggtgaccgg gaatgccaat ggtccgatgg gcgcatggtt agtacatcac
      781 aacgtattat cccacgatgg caacgtgttg cgtgttaaag gccatcaggg gcgcgcattg
      841 gggcgtgatg gtgtaattga tgtgacggtg acaattcgag ataaccaacc ggagaaagtt
      901 accatttccg gcacggcggt gattctattc catgctgaat gggccattga tttttgatgc
      961 cgttgcctga tgcgcttcgc tgatcaggcc tacaaccgga ctaatgtagg ccggataagg
     1021 cgtttacgcc acatccggca acctgacata aattattttc ccgcctccgg gtgcgtatca
     1081 aacgccgcca tcaccagcgc taactcatcc acagtaaagc catgtttcgc atcatctacg
     1141 cccagaccaa actgttcctg catcacggca tataacgacg ccacatctgg tagatttcgc
     1201 tgctcaactg cgtgcccatt ttcataatgg gtaaaatgaa aattggtcag cgttagcttg
     1261 ccaccgtccg gcaaatggcg acacatcagc aaatgatggc gaaaatgcga ctgcggccag
     1321 tgcgccgacc agaaattgcc catcacataa tcgctttgtt gctgctcgca gagatcaaaa
     1381 cggtacatcg actgccagtg ctgatgatga ttaaactgca atacccaatc atcaccctct
     1441 tgcaacaacc gatactctcc gtgtggcgtg gtttgcatga tatccggcat taaacgaatc
     1501 ggcgcggtta gcgtctgccc accgaaaccg acatcagcaa tccatttttc cccttccagt
     1561 tccaccaaca gcaaacgatg ggtgcgcggc ggtaatgctg gcggatttga taacactacg
     1621 cgacctaaca agctgcgaac gttaaacccc agctcgcgta acacccgctc aaacacgcca
     1681 ttctgctcaa aacagtaacc gccacgacgg gctatcacca gtttatcttc cagcgattga
     1741 tcatcaagct gtatttccct cggcagcaaa acgtcgagat tttcaaacgg aatggtgcaa
     1801 ttgtgtttca ggtgcaatgc acgcagcgta tcaatattgg ccgcagcagc tcccaaccag
     1861 ttaatacggg caaaataatg attcagaatg ggcgtcatca ggcgtttccc ttagtggttt
     1921 tctcaactta taacctggaa tagacgccgc actggttatt ttgtgcgatc gcggacatgc
     1981 atcacgttcg caaatcgcca atcccgttaa tttcgttact tattacgtta cgagctacgc
     2041 ttttaaaaca cccttcatag cgaggcgata tcgtgagcca attcattccc gtcgaattac
     2101 accatgccag ccgtctgtta aatcatggac ctactgtctt gatcaccagt ttcgatgagc
     2161 aatcccagcg gcgtaacatt atggcagcag cctggtctat gcccgtagag tttgaaccgc
     2221 cacgcgtggc gattgtggta gataaatcga catggaccag ggagttgatt gaacgtaacg
     2281 gtaaatttgg catcgttatc ccgggcgttg cggcgactaa ctggacgtgg gcggtgggaa
     2341 gtgtgtcggg tcgtgaagaa gataaattta attgctacgg cattccggta gtgagaggcc
     2401 cggtgcttgg tttgccgctg gtcgaggaaa aatgtctggc atggatggag tgtcgcctgc
     2461 tatctgcaac ttccgcgcaa gaaaaatacg atacgctatt tggcgaagtg gtatcagcag
     2521 cggcagacgc acgggtattt gtcgaaggcc gctggcagtt tgatgatgat aagctcaata
     2581 cgttgcatca tttaggtgct ggcacgtttg ttaccagtgg taagcgagtt accgcgggct
     2641 aaagaatcat atgcagcaac caagttgttt aagcaatgtt tccatatgag ttaaagcata
     2701 ttttatctca cgattgcata tttttagtga agattaattt atcctcacca acatcatagt
     2761 gattgcgtat tatcgcctca ttgataaagc ccttttttgt ataaaatgcg cgggcggcac
     2821 taaaatcgat atggctggcg gtctctacta atagtagtct tgcctgttta attgtgagct
     2881 cttttctatt tcgttaatca gcagatgtct cactccctgg cgctgatggg aaggactgat
     2941 ccagagcatt aataaatttc agacatcatt tgtcatcatt tcagaagcac agtaggcaat
     3001 ccctgccagt cccaagtgtt ctgcactaaa ccataaatcg gcagattgac ctgagaaata
     3061 atgtgttagt gttttttgta catgatgaag gccatcttga tcaaattgat ccctgtcaag
     3121 cagcatatcc attaatggtt caacatctga aggtttgact ggtcgtataa acatggaact
     3181 ccttatttat tgatgaatat ttgaaaattg tttgcatgga acgcggttaa cctgaatttt
     3241 cagcggaagt ttcactcaaa ggctttatta atccgctcct tatgtccttt tacagcaaat
     3301 aaataattac ctccctgttt ttgtatcttc tctgcaatat ctttctggca acccatcgca
     3361 tca
//
LOCUS       DAWDRC010000450         3352 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11227_length_3352_cov_2.243555, whole genome shotgun sequence.
ACCESSION   DAWDRC010000450 DAWDRC010000000
VERSION     DAWDRC010000450.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11227_length_3352_cov_2.243555"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          846 a          870 c          807 g          829 t
ORIGIN      
        1 aataatattg ttattttccc ttcagattag tgcagccatt actggcaagg aaagtgaggt
       61 tgtttcacca ttattaatgg atgttaatcc ttcactaaca atggaaaata tttctgaatt
      121 atccacatca tcggaaccat cgcaacaggg ggtatttcct gtaatatgta ctcgacttca
      181 tcctggctcc gtaatgaaac gccagttatt aaccggttgg gggcctgttt ttataattgg
      241 tgatgatcct ttttctctgc gctggatgag tgaacatctg gagatcctta aatcgctaaa
      301 tgcccttggt ttggttgtta atgttgaatc agtagaacga atggaggtat tacagcagcg
      361 ggctgatggg ttattgttat tgccagttat ctgcgataat tttgtgcaag ctctgcaatt
      421 aaatgcttat ccggtattaa tcacagagat ggaaatatca caatgattaa taacataatt
      481 actgctaata atatggtgat tttgaaaatg gtaatcctgt tcacgacacc ataatcagag
      541 ttatgtcctg tatccgttca gtaaaatttc acgaatgctt tattaaccgg atgcgtgaat
      601 gtcattcttc agacgacata gacgtcattg cgattgatgg aaaagcactt ccacactcct
      661 gtgataaaag tcgtcgcagg agagcgggct ttcgtagtct tccccgactc tccccggccc
      721 taaacacaac ccccactcac cacaacctaa actcatccgc atcctgccat gccggaaact
      781 tttctctata ttcccgcaat gccatcatcg acagctccgc atcaatgcgc gttgcctgat
      841 gcgcgtcggc agtagcgata atctcgcctt gtggattaat cacccggctg tcaccgcgat
      901 aatggcagcc gttgccatcg ctgccgacgc gattacatcc cgctacatac gcctgattct
      961 caatcgcgcg ggctgtcagc aatgcctgcc agtgcagaga acgcggtgca ggccagttgg
     1021 caacgtagat ggcgaggtca taatcgttga gattgcgcga ccacaccgga aaacgtaagt
     1081 cgtagcacac cagcggcaaa atacgccagc cgcgccattc cacaatcact cgctcattgc
     1141 ccgctttata atgcagatgc tcatctgcca tgcggaacag atgacgctta tcataaaaat
     1201 gtaccgtgcc gcccggctca accagcaaaa agcggttaac cgaatcagac tccgtttgta
     1261 atgcaacact gcctgcaatc agcgcattgc actgctgcgc tttagcggtc atccagctca
     1321 ctacgtcatc ttgtgctagc gacgaagctg ccgcttccat ggcaaagccg ctggtaaaca
     1381 tctccggtag aacgatcaca tcgcgcccgg taataccttc cagttgacga tcaaaatggc
     1441 gcaggttggc aggaccatcc atccacacca gtggttgctg caaaagcgta atcttcaaac
     1501 caggcacggt gtacaactcc tttatgcgaa gggttttata actctaacac cttatcaggc
     1561 agttgcctta gcgcagaata aattgataac aaatgctgat attggaaata tctgatttgc
     1621 aaattatcgt gttatcgcca ggctttaggg agttaatcac atgggcagga taagcccggg
     1681 aggaatgatg tttaaggcaa taacgacagt cgccgctctg gtcatcgcca ccagtgcaat
     1741 ggcgcaggat gatttaacca ttagcagtct tgcaaagggc gaaaccacca aagcggcgtt
     1801 taatcagatg gtgcaagggc ataagctgcc tgcctgggtg atgaaaggcg gtacttatac
     1861 tcccgcacaa accgtgacat tgggagatga gacgtatcag gtgatgagcg cgtgcaaacc
     1921 gcatgactgt ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta atcagatgac
     1981 ggggctgttc tcgactattg atgagaaaac gtcgcaagag aaactcacct ggttgaatgt
     2041 gaacgatgcg ctttcgattg atggtaaaac ggtgctgttc gcggcgttga ccggcagctt
     2101 ggaaaaccat ccggatggct ttaattttaa ataattagcg gataaagaaa cggagccttt
     2161 cggctccgtt attcaattac gcggcttcaa ctttccgcac tttctccggc aactttaccg
     2221 gcttcgtcgc cagctcttcc ggatcaaagt catcaacgtt aatactgcgc agacggcttt
     2281 cttcagcttt caccagaata gcggcttcat ctttatcaat cagccccttc gccagcgcgt
     2341 tgtgcgccag ttcatccaga cgggtaaacg gcaggttttt acccagctct ttacagatcc
     2401 gctgatgaat cgggtcggcg gcaatcacat ccactagcgc ctcttccagt aaaccaaccg
     2461 ggttatgctc gctcggcgtc aggtactgac cgcgaccaat gcgggaacgg gtggcgttcg
     2521 gcacttgtaa aatcttcgcc accttatgat ccagcttgtc agacggtgcc agataatgac
     2581 gtccggtcgg gaagatcacc acattcagca gccctgcaac cacgcggttc gggaagtttt
     2641 gcagtaaatc atccatcgcc tgttcagcct gatacagcgc atcctgtacg ccccagtgca
     2701 ccagcggcag gtcggcttca ttacggcctt cgtcgtcata acgcttcaga acggcagagg
     2761 cgaggtaaag ctgacttaaa atatccccca gacgtgccga gatacgctca cgacgtttca
     2821 ggctgccacc cagtaccgcc atcgagacat cagaaagcag ggcgaggttg gcgctcaggc
     2881 ggttaagatg ctgatagtag cgtttagtgg catcgccggt tggcgtgctg ctggttaaac
     2941 cgcgcgtcag gcccagccag aagctgcgaa ctttgttgct accgacgtga ccgatatgtt
     3001 tgaacagcag tttatcgaac gcgttaacgt cattgttctt cgccgcttcc atctcttcca
     3061 gcacgtacgg atggcaacga atcgccccct ggccgaagat catcatactg cgggtcagaa
     3121 tgttggcccc ttcaacggtg atggcaatcg gtgcgccctg ataagcacgg gccaggaagt
     3181 tgctctgccc gagcataatg cctttaccgc cggtaatatc cattgcatca ataattgact
     3241 gctgcccgcg gtgggtacag tgatacttaa cgatagccga cagcacggca ggtttttcac
     3301 cgagcataat gccgtaggta atcagcgatg ccgcagcatc catcacgtag gc
//
LOCUS       DAWDRC010000451         3338 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11288_length_3338_cov_2.424307, whole genome shotgun sequence.
ACCESSION   DAWDRC010000451 DAWDRC010000000
VERSION     DAWDRC010000451.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11288_length_3338_cov_2.424307"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          834 a          806 c          803 g          895 t
ORIGIN      
        1 tgctgcaccg gctgcgttgg acgcggagca tgttgcggcg gtacctgcgc ttcaggcggc
       61 tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg gtgttgcggc
      121 gacggacgag cagcctcatg ctcctgggcg ttagccgggg catgattcac gcggtgaaca
      181 cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc gtcgtcacgt
      241 tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc tttacggctg
      301 gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat gattaatatc
      361 agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca ccacggcaaa
      421 catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta ttaactttca
      481 cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat cctaactgtc
      541 cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca aaaggagctc
      601 atcctgatta tggtttcatc attcacatct gccccacgca gcggtttata ctattttgcg
      661 caaggctgga agctcgtctt gcaacctggg attcggcgtt tcgttatttt accgctgctg
      721 gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacgcaact cgatgtctgg
      781 atcccgacgc tcatgagtta tgttccggac tggttacaat ggctgagcta tctgttgtgg
      841 cccctggctg tcatctctgt gctgttagtg tttggctatt tcttctcaac gattgctaac
      901 tggattgctg ctccgttcaa cggtttattg gctgaacaac tggaagcaag actgactggc
      961 gctacaccgc cagataccgg aattttcggt atcatgaaag atgtgccgcg aatcatgaaa
     1021 cgcgaatggc aaaaatttgc ctggtatctg ccgcgcgcaa ttgtattgct aattctttac
     1081 ttcatccctg gcattgggca aaccgtcgcg ccggtactgt ggttcctgtt tagcgcctgg
     1141 atgttagcca tccagtattg tgattacccc ttcgataacc acaaagtgcc gtttaaagag
     1201 atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc tttaaccagc
     1261 ctgtttacga tgatcccgct gcttaatctg ttcattatgc ccgttgccgt ttgtggcgcg
     1321 acggcgatgt gggtcgattg ctatcgcgat aaacacgcca tgtggcggta acaatctacc
     1381 ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc ttattccatc
     1441 ttgcatgtca ttatttccct tctgcatata gatatgctaa atccttactt ccgcatattc
     1501 tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac aggccatgag
     1561 taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc gcctgaatcg
     1621 catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct tcagcgttaa
     1681 gtgccgtatc ggtgctaaca tgatttggga tgccgaaaag cgcggcgtgc tgaaaccagg
     1741 cgttgaactg gttgaaccaa ccagcggtaa taccgggatt gcactggcct atgtagctgc
     1801 cgctcgcggt tacaaactta ccctgaccat gccagaaacc atgagtattg aacgccgcaa
     1861 actgctgaaa gcgttaggtg caaacctggt gctgaccgaa ggtgctaaag gcatgaaagg
     1921 tgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc tgctgctgca
     1981 acaattcagc aatccggcaa accctgaaat tcacgaaaaa accaccggtc cggagatctg
     2041 ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg gcggtacgct
     2101 gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta tctctgtcgc
     2161 cgttgagcca accgattctc cagttatcgc ccaggcgctg gcaggtgaag agattaaacc
     2221 tggtccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc ttgatctcaa
     2281 gctggtcgac aaagtcattg gcatcaccaa tgaagaagcc atttctaccg cacgtcgtct
     2341 gatggaagaa gaaggtattc ttgctggtat ctcttctgga gcagctgttg ctgcggcgtt
     2401 gaaactacaa gaagatgaaa gctttaccaa taagaatatt gtggttattc tgccgtcatc
     2461 gggtgagcgt tatttaagca ctgcattgtt tgccgatctt ttcactgaga aagaactgca
     2521 acagtaatgc cagcttgtaa aaaatgcgta aaaaagcacc tttttaggtg cttttttgtg
     2581 gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac tggtatttaa
     2641 ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa agctgaatcg
     2701 attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac gtacgaaacg
     2761 tcagcggtca acacccgcca gcaatggact gtattacgct cttcgtgcgt cgcgtctgtt
     2821 aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact ttagttccac
     2881 aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac cattaccgct
     2941 ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa gggcttcact
     3001 tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagctt gtttaaactg
     3061 cagactctgg gcctgactca aggtaccgtt gtgactacct tcgcagaagg cgaagacgag
     3121 ccgaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt tcccgggttc
     3181 ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta gcatccccgg
     3241 gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt gaccggaaaa
     3301 aaatttctgc cgaccaggtt gatcaggaag ttgaacgt
//
LOCUS       DAWDRC010000452         3331 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11317_length_3331_cov_2.079976, whole genome shotgun sequence.
ACCESSION   DAWDRC010000452 DAWDRC010000000
VERSION     DAWDRC010000452.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11317_length_3331_cov_2.079976"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    440..449
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1438..1447
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3115..3124
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          752 a          911 c          858 g          780 t
ORIGIN      
        1 acacggggcc agcggactac ggaggcggta atgcgcctgc ttggcgggca aaaagaagat
       61 gcggaactgg acgccgaaac cgcgtccatg ctggtggatc atggcaacca gcagatcttt
      121 aatccgcagg aacggcggat gattgaacgg gtacttaatc ttaaccagcg taccgtcagc
      181 agcattatga cgtcgcgcca cgatattgag catatcgatc tcaacgcgcc ggaagacgag
      241 atccgccagt tactggagcg aaatcagcat acgcggcttg tcgttactga tggcgatgac
      301 gcagaagatt tgctcggtgt tgttcacgtt atcgacctgc tacaacagtt actgcgcggc
      361 gaaccgctca acctgcgggt gttgattcgc cagccgctgg tgttcccgga aaccttgccg
      421 ttgttacctg ccctggagcn nnnnnnnnng cacacacttt gcttttgtgg tggatgagtt
      481 tggctcggtg gaagggattg tgacattaag tgacgtcact gaaaccattg ccggtaactt
      541 accgaacgaa gtggaagaga tcgacgcccg ccatgatatt cagaagaatg cggacggttc
      601 ctggacggcg aatggtcata tgccgctgga agatctggtg caatatgtgc cgctgccgct
      661 ggatgaaaaa cgtgaatatc acaccattgc cgggctattg atggaatatt tgcagcgtat
      721 tccaaaacct ggcgaagaag ttcaggtggg gaattatttg cttaaaacgt tgcaggtgga
      781 aagccatcgc gtgcagaagg tacagattat tccgctgcgt aaggatggcg agatggagta
      841 cgaggtgtga tggcatgtag gcctgatgcg acgctttacg cgtcttatca ggcctacagg
      901 acaggtcacc tggtaaccta catcttctcc agcaacttct tcacatcttt gccattgcgg
      961 gaatctttat tccgctcggc ccagtcgtta agacggcgtt ttgcttcatc ctgtagatgt
     1021 ttacgcagta attgatccac ttgcaggctg taattaagtt gctgccagtt gccataaacc
     1081 cgcagcggta ctggcgtttc tttgaggaaa tcaatcagtt tgctttcccc gttccagcca
     1141 cccacgaccc gaatatcaaa ctgggtgtca caggtttgat ctgccagatt caacatgcct
     1201 tcccctgtca gcgccagcac tggcgattga ccttgcatgt cgtttaacgt cacgacgcca
     1261 tccttcaacg tcaaatcggt ggtaaagcgg tcaagacgcg tcacgttatc gaagttttca
     1321 gcggccttca catcaccacc attacgttct accgcttgct gaatcatctg ctggaagttc
     1381 atcccttcca tgcgtgtatc agtcatttcg acatgtgcct gtccttgcca gttgtggnnn
     1441 nnnnnnngcg tcggcatcta tgtcagcacc ggagaaatca ccagccagtg acatctttcc
     1501 ggtcaacgaa atcggatagt taaacgcctt cagaatggtg ccaatctcaa cgttttccag
     1561 ccgtggctgg aagtttatcc gcggatttat tgatgtcgcg tccaacgtgc ccggcagtga
     1621 aacctgtcca ccgttaagtt tgccctgcag ttgagtaatt tccagcaaac ccgacttgtt
     1681 ggtcatttgc gtggcaacat ctgtaaaatt cattccgcgc cagcgcacgt tactggcctg
     1741 caacaaaata tcagccgtaa agccttgcag tccctgatag gccggttcat caatacgcga
     1801 agaaatgacc gggcgcggca acgttgattg gctctgccct tgctgcgcgg caccgttctc
     1861 accattcgct gtttcattaa gggggatgag gttgtcaaga ttcagttgcg ggaattgcag
     1921 tctcagctgc cattccggtt tctctgtcaa cgtgacctgt gcttgcccgc tcagcgtact
     1981 gtcattggcg gtcaaactaa tttggttaaa tgaaaggtgt ttatgtgact cctgccactg
     2041 gacctggaaa ctcccctgcc cctgaatacc ttgttttggt aaatcggcac cctgcaactg
     2101 ccagttaatt tgttcaatag ccgccgttaa atcatgcgga taatcagaag catccaccgt
     2161 accgttaagg gatatcgtga gatcgcgctg atcgcgatta acccgcccgg aaaactcaaa
     2221 tgagccacga tgttgaggat cttgttccat ttgcaggcgg atattgcgga tggtcacctg
     2281 ctcgtcatct tcatgttgga acaccagaac gctgtccgcc accttaagac tggatatatc
     2341 aaacgaccat ccgcgatcgt ctgacagatc cggcaaggta ttgtcgcgcg gtgcaaccgg
     2401 agcgtcttca ctgcgcaccg cttccgtctg cggcgtcagt tggatcactg ccccctttag
     2461 catcacctgc ttaacgctca gttgatgact cagtagtggt aaaagcgcca cgtccagacg
     2521 catgttgtcg gcgcgaacca gtggctggct tgcgccctgg gcggtgagag acattcgccc
     2581 ggagaggata ctaagctgcg gccagacgtg ccaacgcagt ggcccgtcga gctgcaattg
     2641 ataaccgcta cgtgcagcaa cttgcttgac catatagtcg cggaaatcat tcggattcac
     2701 cagcaacact aacgcagata acccggccac cagcacgacc aggagtatca tcagcgtcgt
     2761 cagaaatcgt ctcatggtat cctcaatggg cctgaattag tctttatcga ttcggctggc
     2821 taccgcgccc tgctggttgc gatatttcgc atcttcacgg cggttgtaag gtcgcgccgc
     2881 cgggccggag agcggctcaa agctcagcgc accaattaac atgcccggac gcagcgccag
     2941 cggcagctta ccggagttgt aaaactccag cacaatgcag ccagaccagc ccggatcgat
     3001 acggtgcgcg gtgacgtgca ccatcagccc cagacgcgcc agtgaagaac gcccgtccag
     3061 ccagcccacc agatcggctg gcagcgtcac cgactccagc gtcaccgcca gcgcnnnnnn
     3121 nnnnctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa ggtacgaaat
     3181 ttattgccca ggcgcacatc caccgtcgcg ccgttaatac gctccactgg cggacgtggg
     3241 ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca cagacgcatg
     3301 gcattttctc ctttcgagca tcactccctt a
//
LOCUS       DAWDRC010000453         3328 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11331_length_3328_cov_1.786740, whole genome shotgun sequence.
ACCESSION   DAWDRC010000453 DAWDRC010000000
VERSION     DAWDRC010000453.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11331_length_3328_cov_1.786740"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1240..1249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          773 a          972 c          850 g          723 t
ORIGIN      
        1 ccagtaattc agtgccctcg cgcatactcg ccagtggcgt ttttaattca tgagataaat
       61 gtcttaaaaa ttgatggcgt tgggattcca gccatgacag gcgctcactt aaccaaagaa
      121 tacgttgccc aaccgagcgt aactcgctcg gtccactgaa cgagacgcta ttgcccagag
      181 aacgcccttc ccccagccgg ttgatcatgc gctcgatatt tttcaccgga ccgataatca
      241 tcctcgtgaa aagcagtacc atcaccagac tcaccagaaa tagcaccagc gattgccaac
      301 caaaatattg cccacgttcg gcgatttcac gctgaagttg ctgcccacga gagaacacca
      361 ctgtgcgcgt ggcctgcacc atttcggtat tggcactggc aaaggcttcc agacgcgcgg
      421 cggcagcagc atcgggaccg ctgttgttac actgaagttg agccagattg ttcaaatcct
      481 gacgtaatgc ctggtagagt ttatcgtccg gcagcacgcc tgcgtgggca tcgagcattt
      541 cgctgtaacg cttgcgctgg ctttgataaa ccttcgcaag cgtagggtcg tccagcacgc
      601 agtactgacg atagctgcgc tccatctcca gtgccgcgtt ggtcatcgct tcactgcgcc
      661 gggcatcaat aagcgtagtg cggttaacca gcgccgcctg atcgctaagt gcattcaggc
      721 tttgccaggc ttgccatgcc agcaccaaca ggggcagcag aatcagcaaa aatgccagca
      781 ttaccagttg tcgtaatgag cggggaaaaa cgggccagcg tttcaaggcg ttactctcgt
      841 cagacgcgaa tagcctgatg ctaaccgagg ggaagttcag atacaacaaa gccgggaatt
      901 acccggcttt gttatggaat aaggcggtgc ctaactcgac gtttcgcccg atggttgata
      961 tagctacgct gatatcagaa gttggacggc aggcaccttg ttgtgcgtca ttcgaatttt
     1021 atgtagcacg tcccgaaggg gctgacataa gtcggtgaat gagccactgc ttactattat
     1081 gcagtaactg tgccaataaa gaaaatagtt tggtaacata ctgattatac gctgctttga
     1141 ggggtttatg gcttctccgc tatttgaatg taaatcagcc actgtgtctc taaaaagaga
     1201 caacttaaga taaacttatt agataatatt aaaatcaatn nnnnnnnnng ctcataatag
     1261 cggttggtgc ttaatgccgg atgcggcgtg aatgcctcat ccgggctgca ataccaatgg
     1321 gttgacgact taccccaact gcttacgtgc attgcggaaa atgcgcatcc atgggccatc
     1381 ctcgccccag ttttccggat gccaggagtt gctgacagta cggaaaacac gttccgggtg
     1441 cggcatcata atggtgactc gaccactttc agtcgtgact gccgtaatac cgttcggtga
     1501 accgttcggg ttagccgggt aggtttcagt gactttgccg aagttatcga catagcgcag
     1561 tgccaccagc cctttgcttt ccagtgccgc cagatgcgcc gcatcgcgca cttcaacgcg
     1621 cccttcaccg tgagagacag caatcggcat ctgcgagccc accatcccct gcaacagcag
     1681 cgacgggctt tgggttactt ctaccagact gaaacgcgct tcaaagcgat cggaggtgtt
     1741 acgcacaaaa cgtggccaca actcgctacc cgggatcagt tcacgcagat tagacatcat
     1801 ctggcaaccg ttacataccc ccagcgccag cgtttgcgga cgatggaaga aggttgcaaa
     1861 ctcatcgcgt acacggtcat tgaacaggat agacttcgcc caaccttcac cagcgcccag
     1921 cacatcaccg taggagaaac caccgcacgc gaccagggcg tggaaaccct ccaggcccgt
     1981 gcgtccagcc agcagatcgc tcatatgcac gtcgatagca tcgaagcctg cacggtggaa
     2041 agctgccgcc atttcaacat gcgagttcac gccctgctca cgcagcacgg caactttcgg
     2101 gcgcgcgcca gtggcaataa acggtgctgc cacatcttcg ttgatatcga acgacagttt
     2161 tacattcagg cccggatcgg cgtcgtttga tttcgcctgg tgctcctggt cagcacactc
     2221 cgggttgtca cgcaggcgct gcatctgcca ggtcgtttct gcccaccaga cacgcaacgt
     2281 agtgcggctt tcgctaaata cagtctgccc gttggcggta atcacaaaac ggtcaccgga
     2341 aaccgcctgc ccaacataat ggacgcaatc agcaagccca tgctgtgcca gtaccgcttc
     2401 aacggcttca cggtcagcgg ctcgcacctg aatcaccgca cccagttctt cgctaaacaa
     2461 tgcagccaga cgatcgtcac ccagagtggc gatatctgcg tcaatgccac aatgaccagc
     2521 aaacgccatt tccgccagcg tcaccagcag gccaccgtca gagcggtcgt gatacgccag
     2581 cagcttacgc tgtgcaacca gcgcctgaat cgcgtcatag aagcctttca gttgggcaac
     2641 atcgcgtaca tctgccggtt tgtcgccaag ctgacgataa acctgcgcca gtgccgttgc
     2701 gcccagcgcg ttattgcctt tgcccaaatc aatcagcaac agtgcgttat cttcggtaga
     2761 aagctgcggc gtaatggtgt gacgtacatc ttccacgcgg gcaaatgcgg aaatcaccag
     2821 cgacaacggc gacgtcattt cgcgctcttc gttaccttcc tgccagcggg ttttcatcga
     2881 catagagtct ttacccaccg gtatcgtcag gcccagcgcc ggacaaagtt cttcgcccac
     2941 agctttaacg gcttcataca ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca
     3001 gttggcggaa agtttaatgc gtttgatatc gccaatttgt gttgcggcga tgttggttag
     3061 cgcttcaccg actgccagac gggcagaagc ggcgaaatcc agcagcgcaa ctggcgcacg
     3121 ttcgccaatt gccatcgctt cgccatagta gctgtcgagg ctggcggtag tgaccgcgca
     3181 gttagcgacc ggcacctgcc acggccccac catctgatcg cgcgccacca tgccggttac
     3241 gctgcggtca ccgatggtca ccaggaaagt tttctccgcc acggtcggca gatgcagcac
     3301 gcgtttcacc gcgtcagcga tggtgatc
//
LOCUS       DAWDRC010000454         3309 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11399_length_3309_cov_2.169637, whole genome shotgun sequence.
ACCESSION   DAWDRC010000454 DAWDRC010000000
VERSION     DAWDRC010000454.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11399_length_3309_cov_2.169637"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1941..1950
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2383..2392
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          672 a          895 c          863 g          859 t
ORIGIN      
        1 aatattatct gcataatacg tcagtgagtc ggttgcatag acaagggaat aaaaacctta
       61 attatcaacg tcactatatt aagattaccc gcctgctgga gaaattaaat cgaaattatg
      121 ccgacaaaat tacgatttat ccggaatttc atcagcaaat aacttacgaa gcattgcgtg
      181 tttgccatgc ggtgcgcaaa gagccggata ttattacccg ccaacggatg attgctgaga
      241 tatttacttc cggtatgtat aagcgcctga ttaccaatgt gcgcagcgtg aaggtgggtt
      301 accaggcgtt actgtggtct ttccgcttat ggcaatggcg cgacaaaacg cggtcgcacc
      361 atcgcattac gcgtagcgcc tttaatttgc gctagcgtta agttttgccg attcgggctg
      421 tttgccccag ccttgccact gatgctggaa gtaaacgatc agcgtgctct ggctgatgct
      481 ttcgcttaat acttcaaaga agcgattagc ataaacaggt tctatcggct tcttcggttt
      541 gcacagcttc acgccacgga atggattacg cggtgtgtcg ggcacagtgt ttttcggcgg
      601 tgtagacgta tcaacctgcg gttcattcag caagctgctg gctttcacca gcgtaatgtc
      661 tggcggcagg ttataaacca tctgttgtag cacgcgtacc gttgaagggt gaggatgccc
      721 aatggcaatg gttgaaccgt tgcgacgcgc caggtcaatt gcgcgattga attgcacacg
      781 gatgtccgct tcgttctgcg aatcgtcgag gaacaccttc cgtttgatca ccttcacgcc
      841 agtgccttgc gcggcgcgca tcgcctgggt attaccgatg gttacgctgt cgaggaagta
      901 aagattgtaa cgctccagcg cctgcatcac tttctgcata ccaaacaggt tagaggtcat
      961 cttgctgccc atgtggttgt tgatccccac ggcatagggc acgttattga ccgcactacg
     1021 aataatgcgc tcaatttcgt cgctgctcat ctccgggcgt agcgtatttt tctccagcgg
     1081 ctgtttactc agtggtgcca tcggtagatg aatcaacact tcgtgaccgc tgttatgcgc
     1141 tttggtcgcc atctctctgg cgtgcggtga atcgggtaat acagcgacgg agatagcgga
     1201 aggcatcgcc agcacctggt tttcgttgtg cgggcgatac ccaaaatcat caatgacgat
     1261 ggcaagtttg ccagcaatta cgggggagga gagcgccaac agagtggcaa atgcaagaac
     1321 gttacgacga aatggaaaca aaacttatct tcccaaccac ggctgtggat tgaccgcctg
     1381 accctggcgg cgaatttcga aatagagtga aggccgaccc tgaccgccac tgctgcccac
     1441 cagtgcaatt ggctggcccg cgcgaacctg cgaaccaacg ctcaccagtg cgctctgatt
     1501 atagccgtaa agactcatgt cgcctttacc atgctcaacc accaccacca gaccgtagcc
     1561 ttgcagccag tcagccagaa tcacccggcc gtcggcaatc gctttaactt cagtaccttc
     1621 agaggcacca atcaccatcc ctttccagcg taattcaccc tgtagctgtt cgccatagcg
     1681 atgcagcgtc ggcccgcgaa ctggccagaa tgcctgaccg cgcggtgcgc ccagaccacc
     1741 ggtacgggac atcagcgatt tttcgctttc ggtcggcttg taggtggtgc ctttgcgcgt
     1801 cgcttctttc tggcggtcgc gaaccgcctg ggcttcgcgc gcttcacgtt cagcacgcgc
     1861 tttcgccgcg gcttccgcac gggcaatgct gttacgcaga cgggattcgt tggcgcgcag
     1921 ctcgctcaac tgttgctgac nnnnnnnnnn accttgctgg atggaagact ccagccctgc
     1981 cagtgttttc ttacgctcgc tcaacgcctg ggtcagcttc gcctgttggg cgcgctgctc
     2041 atataaaagc gtttgttgct cgctctgttt ctcttccagt tcggcacgct gcatagcgac
     2101 ttcttcacgc gtttgtttca actgagcaat ggtttcttgt cgcgcctggt tgagatagcc
     2161 gaaataagcc tgtaaccgct gcccacgctg gctttcttca ccgctgagaa tcagctgaat
     2221 accggtatgt tcaccctgac gaaacgcggc atccagttgc gcggcgaggc tgcgctcctg
     2281 ggcggctttt tgctgctcta gtttggcaat cgacgcgttc atctcatcaa tctgtttatt
     2341 cagttgatta agcgtgtttt gcgtttcgcg cagtttacgg gtnnnnnnnn nntcgcttct
     2401 tcctgctttt tcaattgtgc gagcaggctt gcgcgttgtt gttgcttttg gcgtaccgcg
     2461 cgctctttcg cggcgatgtc agcctgaatg gatttgagtt ggtcacgctc atccgcgtgg
     2521 gcggaaaagg cgcacaacaa tacgccagcg ctcagaacgc tggcgtagat gatgggcctg
     2581 attgcaaacc tgcgcggttt cacggcccgt gtcatggtat taatcgcctt tcccctcatg
     2641 gggagggatt attccacgat gaacagcggc ttaccagtca tctcttgcgg gatttccata
     2701 cccatcagcg acaacatggt cggcgcgatg tcagaaagtt tgccgccttc aaccgctttc
     2761 acgttcttat caccaacgta aatcagcgga actggcaggt tggtgtgtgc tgtgtgtgcc
     2821 tgacccgttg ccggatcgcg catctgttca gcgttaccgt ggtcagcggt gatcagcagt
     2881 tgtccaccca cggactcaac cgctttcgcc acttcttcca cgcagtgatc cagcgcttca
     2941 accgctttaa ccgccgcttc catcactccg gtgtgaccta ccatgtcgcc gttcggatag
     3001 ttacagataa tggtgtcgta tttgccgctc ttgattgccg caaccagttt ttcggtcagc
     3061 tctgcggagc tcatttccgg ttgcagatcg taggtagcca ctttcggtga gttgatcaga
     3121 atgcgatctt cgcctttgaa cgactcttct acgccaccgt tgaagaagaa agtaacgtgg
     3181 gcatattttt cggtttcgga aatacgcagc tgagttttgt cgtttttcgc catccactcg
     3241 ccgaaggtgt taaccaggga agcgggtggg taagccactg cggttttgat gtcggcagcg
     3301 tattcggtc
//
LOCUS       DAWDRC010000455         3298 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11439_length_3298_cov_2.477953, whole genome shotgun sequence.
ACCESSION   DAWDRC010000455 DAWDRC010000000
VERSION     DAWDRC010000455.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11439_length_3298_cov_2.477953"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1360..1369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3002..3011
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          764 a          863 c          802 g          849 t
ORIGIN      
        1 cgattaacac tcagactgcc agtgttttta acctgcagag tcgtggtagg atccgctacc
       61 acagaaaatc cacacaacag tttgagctaa ccaaattctc tttaggtgat attaaatatg
      121 gcaaaacacc tttttacgtc cgagtccgtc tctgaagggc atcctgacaa aattgctgac
      181 caaatctctg atgccgtttt agacgcgatc ctcgaacagg atccgaaagc acgcgttgct
      241 tgcgaaacct acgtaaaaac cggcatggtt ttagttggcg gcgaaatcac caccagcgcc
      301 tgggtagaca tcgaagagat cacccgtaat accgttcgcg aaattggcta tgtgcattcc
      361 gacatgggct ttgacgctaa ctcctgtgcg gttctgagcg ccatcggtaa acagtctcct
      421 gacatcaacc agggcgttga ccgtgccgat ccgctggaac agggcgcggg cgaccagggg
      481 ctgatgtttg gctacgcaac taatgaaacc gacgtgctga tgccagcacc tatcacttat
      541 gcacaccgtc tggtacagcg tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg
      601 cgcccggacg cgaaaagcca ggtgactttc cagtatgacg acggcaaaat cgttggcatt
      661 gatgctgtcg tgctttccac tcagcactct gaagagatcg accagaaatc gctgcaagaa
      721 gcggtaatgg aagagatcat caagccgatt ctgcccgctg aatggctgac ttctgccacc
      781 aaattcttca tcaacccgac cggtcgtttc gttatcggtg gcccgatggg tgactgcggt
      841 ctgactggtc gtaaaatcat cgttgatacc tacggcggca tggcgcgtca tggtggcggt
      901 gcgttctccg gtaaagatcc atcaaaagtg gaccgttccg cagcctacgc agcacgttat
      961 gtcgcgaaaa acattgttgc tgctggcctg gccgatcgtt gtgaaattca ggtttcctac
     1021 gcaatcggcg tggctgaacc gacttccatc atggtagaaa ctttcggtac tgagaaagtg
     1081 ccttctgaac aactgactct gctggtacgt gaattcttcg acctgcgccc atacggtctg
     1141 attcagatgc tggatctgct gcacccgatc tacaaagaaa ccgcagcata cggtcacttt
     1201 ggtcgtgaac atttcccgtg ggaaaaaacc gacaaagcgc agctgctgcg tgatgctgcc
     1261 ggtctgaagt aatctttctt cacctgcttt caaaggccag cctcgcgctg gcctttttct
     1321 tttacctcac cgcagtggac aggcgttcac gccgcatccn nnnnnnnnna taacatcatt
     1381 cttcctcaac acgttttacc gcaaattatc atcactactg aaatcgatta caccaaccac
     1441 accagccaaa tatttgtaat attttcatat tattattcag ctttcacagt tgttacattt
     1501 cttttcagta aagtcttaat tgcagataac aacctttaat ctatgatgat ataactcaat
     1561 tattttcatg cacttaaatc ataactaaga taaatgttag tgtaagcgat tacactgatg
     1621 tgatttgctt cacatctttt tacgtcgtac tcacctatct taattcacaa taaaaaataa
     1681 ccatattgga gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga
     1741 cgtttttcgt ctgcttcctt gccgctctgg cgggattact ctttggcctg gatatcggtg
     1801 taattgctgg cgcactgccg tttattgcag atgaattcca gattacttcg cacacgcaag
     1861 aatgggtcgt aagctccatg atgttcggtg cggcagtcgg tgcggtgggc agcggctggc
     1921 tctcctttaa actcgggcgc aaaaagagcc tgatgatcgg cgcaatcctg tttgttgccg
     1981 gttcactgtt ctctgcggct gcgccaaacg ttgaagtact gattctttcc cgcgttctgc
     2041 tggggctggc ggtgggtgtg gcctcttata ccgcaccact gtacctgtca gaaattgcgc
     2101 cggaaaaaat tcgcggcagt atgatctcga tgtatcagtt gatgatcact atcgggatcc
     2161 tcggcgctta tctttctgat accgccttca gctacaccgg tgcatggcgc tggatgctgg
     2221 gtgtgattat catcccggca attttgctgc tgattggtgt cttcttcctg ccagacagcc
     2281 cacgttggtt tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg ctacgcctgc
     2341 gtgacaccag cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt ttgcaggtta
     2401 aacagagtgg ctgggcgctg tttaaagaga atagcaactt ccgccgcgcg gtgttccttg
     2461 gcgtactatt gcagataatg cagcaattca ccgggatgaa cgtcatcatg tattacgcgc
     2521 cgaaaatctt cgaactggcg ggttatacca acaccaccga gcaaatgtgg gggacagtga
     2581 ttgtcggcct gaccaacgta cttgccacct ttatcgcaat cggccttgtt gaccgctggg
     2641 gacgtaaacc aacgctaacg ctgggcttcc tggtgatggc tgctggtatg ggcgtactcg
     2701 gtacaatgat gcatatcggt atccactctc cgtcggcgca gtatttcgcc atcgccatgc
     2761 tgctgatgtt tattgtcggt tttgccatga gtgctggtcc gctgatttgg gtactgtgct
     2821 ccgaaattca gccgctgaaa ggccgcgatt ttggcatcac ctgctccact gccaccaact
     2881 ggattgccaa catgatcgtt ggcgcaacgt tcctgaccat gctcaacacg ctgggtaacg
     2941 ccaacacctt ctgggtgtat gctgctctga acgtactgtt tatcctgctg acagtgtggg
     3001 tnnnnnnnnn nttgtggctg gtaccggaaa ccaaacatgt ttcgctggaa catattgaac
     3061 gtaatctgat gaaaggtcgt aaactgcgcg aaatcggcgc tcacgattaa tctcctcagg
     3121 cttcctcccg tcgcggagga agccacctct tgcagtcatc ttttcttcgc tctatcctct
     3181 gccgctatga aaacatcccg tctccctatc gccatccaac aggccgttat gcgtcgcctg
     3241 cgggaaaaac tcgcccaggc gaacctgaag ctagggcgta actacccgga gccaaaac
//
LOCUS       DAWDRC010000456         3292 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11461_length_3292_cov_2.434971, whole genome shotgun sequence.
ACCESSION   DAWDRC010000456 DAWDRC010000000
VERSION     DAWDRC010000456.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11461_length_3292_cov_2.434971"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1116..1125
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          927 a          784 c          602 g          969 t
ORIGIN      
        1 tgtcgataag cactcgactg actataactg ccattcactg tcgcaaccgg cgcgttccag
       61 gttaaattcg ccccagctgt cgtttcattt ccctgatact gataactcag gttgacccca
      121 tagttaaact gatcccggct tccaacggtt cctgataatc ccgtattatt tgaggcaacc
      181 ccctgatcat caaacgtcgt tgagttagac atatatattt gccgacgagg cgtcgtaacg
      241 tcatcacccc aatcaaaggg aatcgatata aaaatattaa aacgtttctc ttcatgatga
      301 ttctcgtcat aagcatggct tgccgcgagg gtatagctta tccttcgcag gttgttggaa
      361 taactcaact gataatcctt actgctgccg ctacgccccc agtaatctcg ccataacgta
      421 cttaaggaca cagaacccca gccttctggc aatgactgac tcatattggc agagaagcta
      481 tttttacggc caaaatcgtt ctggtaataa tcggcaatgt catagatatc gttttcatca
      541 cggcgataat tatctttatt gtttgcccat acgtgatcat taaatgtccg ataatcacgc
      601 gacgaataac gccaggccgc cagaccaaaa cgtgtcgacg tttggctcac aaatttgttg
      661 taggcaattt gataactttg cccgtcaaac acatcgccgt tgtcttgttt actatgcgac
      721 ttcgtggcat cgacggaaat ggcaccaatg cgtgtattcc agccagtccc caaagtgaac
      781 gcgtaataat tattcgcgac catcgagcca ccatacagcg ttaataagtt attaaaacca
      841 tactgatgac ccgcctggac aaaatcactt tgtttgctcg ccccttcaat atggctacga
      901 cccgccgcaa aatcatattt cgacacgccg ggttgcagca tatttggtac cgctgcataa
      961 ggcaccagat aggtggttac cgaaccgtcc gcctctttca cgctgatatc aagatctgct
     1021 ccacctccgg ccaactgcaa atctgtaatc gcaaacgggc caggaggaac ctctttctga
     1081 taaaccacaa aaccattctg ttcaatagtt accagnnnnn nnnnntgtgt acatatcacc
     1141 cacacgaagc gtgccgagaa gttgggcaaa tccacgttcc agatacaggg tattgctttt
     1201 ccacacccct ggattgttat ttgttttact gaaactggca tcagaatgca gttgccaccc
     1261 caataaattt aacccgctgt taaaacgtac atatgtactc ttgctattac ccgacgcttt
     1321 atagtcgctg taatactgac tcacataata agaggtataa aacgcattaa taccccgctc
     1381 ccagttttca ggtggtacat agccactttc cagttcttcc acccaggcct gcgggacact
     1441 gaaatcgaga cgaaaaactc cgatatccca ggtatagctc ccgccctgaa taagttgctt
     1501 aaatgttaaa cattgcttac cgctggcaaa gctatcggta ttaatgccta accgcttgat
     1561 catttccctt gataaacatg tttcttgcgg gttgtcttta acaatgatct catatttccc
     1621 acgccattgc ttattgacat aaatatcgat gtcatacggc cccggcaagg gctgattatc
     1681 ttcaagacga atatttgaaa cctgctggtc tttcattcca cctatcatga aatgggtatc
     1741 aaaggtttct tcagctgcat aagcttcaat gccgatcaat aacgctacga ttgctgatgc
     1801 aagtggggtc attctcaaca taggcagctc ctgcaattaa attttgtcac taatataatt
     1861 gccatggtca tcgataatgg tcagatgcca gttatttgca ttattacttt tgacattaac
     1921 actctgactt tctaaggggg caatcataat agtttcataa ttgactttga cattattagc
     1981 tttgacatcc gaaatcgtca cccaattagc tgagtcattt tttatcacca atccattgcc
     2041 actgcgattt accagtaact ttttaaaggt cactttattt accggagcaa tacccgctgg
     2101 tcggtaaaac aacttaattc tgttttgcat cgcaaacttc agtgcattct taccttcttg
     2161 ttctggacta tttggtggaa tatccagaac attcagataa aaaatactct ctttattagt
     2221 gggtagttta ttcggcataa ttttaatttt tacttgctgc ccggaatttg cccctatttt
     2281 tgccactggt ggcgttaaca tgaaaggaac ctgaattttt tctggtggta atgacgtatc
     2341 gccatcatca atccacgcct gcatcagcga agaacggttt ccctggttca tcaactgcac
     2401 catcacttct ttgtgctctg ccgggtaaat aatacgcgtc ccgtagataa caataccggc
     2461 atgtgcagga aggaccatag aagaaatgag taaagctaat aatcctttca tcttaatgcc
     2521 tcttaaatta aaaggccgcc atggagatag cggccatgtt aattacctga agaatcttgc
     2581 aattttattt attaatcctg cgccacacgc ataatcaggg aagataacac atcacctgaa
     2641 ttcactgagt tatctgtcag tttcaggtat tttgctttaa acggaatggc atattgaccg
     2701 tccgctgttt tataactctc gatgttgaaa ggtgcagaaa catccaggac atcgctgtta
     2761 tcacctaaat agagataata acccacggta tcgttgctac tggctatcgc cttatcgttt
     2821 ccggttattt tatcgttggt aatcaacgag agttttacac cagcaacttc tttcggacag
     2881 ctaattttta acatgctgct ggttacaccg actgcaccat cataggtcga gtatttagct
     2941 ttaccttcaa agaccgtagt tggtacagtt ggcatattaa tacttactga atcgccacct
     3001 gcttcgaatt tacacggaga ttctgcaata ttccctttaa tggttaatgt tccggtatca
     3061 acgtctgcgg caaatacccc agcgctcata aaaaaagaag aaaaaacagc ggcagcaata
     3121 attgaacgtt tcataatatg ctccttaaca aaatctattt ttggttgtta agagtctgta
     3181 tctcttggaa acaaatttct cacaccaaaa aagttgccgc aatgtaaaca cattgtttta
     3241 ttgatttaaa gcaaattaac cattgcaata taaacacaac aaatacttaa gc
//
LOCUS       DAWDRC010000457         3289 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11473_length_3289_cov_2.686766, whole genome shotgun sequence.
ACCESSION   DAWDRC010000457 DAWDRC010000000
VERSION     DAWDRC010000457.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11473_length_3289_cov_2.686766"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          794 a          913 c          809 g          773 t
ORIGIN      
        1 caatctgatc attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta
       61 cggtgccaat ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa
      121 tctgaatgcc gccgccgcaa cgtttaatcg tgttgatgtc cgtcgtccgt cgctggcgct
      181 aactgccaac agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat
      241 tctggaagcc accgccagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa
      301 tggacgcggt gtgccggtga atattttgca acattgggga tggcctgaat taccgttgac
      361 tggcgacggc aatattcagc ttaccgccag tggcgatatt caggtcaatg tcccgctgaa
      421 acctacggtt agcgggcaat tgcatgccgt gaatgccgca aagcagcaag tgactcaaac
      481 catgaatgcg ggcgtagttt ccagtggtga agttacgtcg acggagccgg tgcagtaaaa
      541 ataaggacgc tagcggatat cagcgctagc gttccccgtt attagcgcaa aattccatac
      601 tcaccgcaca ataaccattt caccggttca tcttcttcta tcgccggaaa acgtcctccc
      661 ggctcaagaa aataattatc gtgttggtca tcatttacca tcgagatttc ccagccgaga
      721 acaaacgctt tttccgccca gaagcgatga tacagacgcg gtggcaggca aatgccctgc
      781 cccggcttta gcaccagcgt ttcaccaggt ttcatggtac gccgctgacc atcaacactc
      841 acggtaatga ctcgttgatc atcacagaga ttggcttcgt tggcccaggc caactgcatg
      901 cataagtctc caccgccccg attgaggata tcttccattt tgtgggtgtg aaaatgccag
      961 ggagtctgct gatcttgttg gatctgcaac attttctcag catacggttt tggatatgcc
     1021 gccgacccca gcgcgccgtt gcgcatcgtt agcagtgtta gtcccgtttg gttgaaatca
     1081 ccacgaccaa aatcggtgat atcccaaccg agctgtaagt cgaccacttc ttgccagtta
     1141 tcccgttccc gctgcagcca gtcatccacc gtaaaatagg cgaactctgg caaacaaacg
     1201 cggaaggtct gcgcaatggc atgtgcttta tcaataacat aattaatttg tgaacgtttc
     1261 attggcttct ccgttgcgca tagcaagcca ggcagcgcca cgcattccgc tactgtcgcc
     1321 gtgctgtgct tttacaatgg gagtggtgaa ttgatcggtg aaaaccagtg gagcgacttc
     1381 tgcgttcaga tcggtgataa gcagttccac attcgaaagc ccgccgccga tcacaatcac
     1441 gccagggtca acaacgttaa cgatggttgc cagcgtgcgg gcaagctgtt ggcgaaaacg
     1501 tgccacctgc tgcatggcac aagcatcacc acgttgtgcc agggtgacaa tcgccgcagg
     1561 agtgagcaag ttaccagtca tctgttgata gcgttcacta aaaccgctgc cggaaacaaa
     1621 cgactccacg cagttgtgtt tgccgcaata gcaactgact gatggaccat cttcctgctc
     1681 cgtatagcct ggcaacgtga tatgaccgca ttcggcagca tttccccagg cccctataaa
     1741 cggtcgttgc ttgatggcaa tgccaccgcc gcagcccgta ccaagcgtaa taccaaatac
     1801 cacgtcataa tcttgcccgg caccgtcgca agcttctgat agcgcgaaac agttaccatc
     1861 attggctatc accaccggtt gcccaagcaa ttgctgcaaa tcagcctgta acgcatgacc
     1921 gttaatcacc tgaatattcg cgtttttaat caggccagtg agtggcgaga tactgccggg
     1981 taaggcgatc cctgtcagca tcggtcgttg aacgtcccgc ctaatctgct cgataagcgc
     2041 cacaacgcat gagacaaatt gttgatatgt cgacttttgc gtcgggcagc ggtaacggcg
     2101 aatctcccag ccatgcgcat ccatgacaac ggcggcgatt ttggtcccac caatatccaa
     2161 ccccagatag tgcatatctc gttccttgtt gcgtaaaagg cgggtcaccc cgcccttctg
     2221 gcttagtaca gtgatgcttt accgagcgaa ccgaacagtt tcattttgtg gcgaatgact
     2281 tcccgcgccg cgttaatcgc atccggatag atgacgttcg ggtcccacca gctctcttta
     2341 acgagaattt cacgcacttt ctggaaatag gcgtatttca tatcgctgga gatattgatt
     2401 ttgccgacgc cgagcgtaac tgattcagca atctccgcgt ccgggtttgc cgaaccaccg
     2461 tgtagcacca gcggaatatc aatacgtccg gcaatgtcgc gcaaaatgtc catttgcagc
     2521 ttcggctgca tcccttttgg atagatgcca tgcgctgtac caatcgccac agccagtgtg
     2581 tcggcacctg tacgagcaat aaaatcggcg gcctgcgccg gatctgtgta ggtcacttgt
     2641 gaaacaccac cttctaccga cgttcctgtt tgtccgatgg ttcccagttc accctcaacc
     2701 gatactccaa ccgcgtgagc gaggcgaacc acttctgcgg tcagcgccac gttttcttca
     2761 tacggcagca gcgaaccgtc gatcattact gaggtaaatc cgcagcgaat cgcacgcaaa
     2821 acatgctcta cagaggcacc atgatcgagg tgcagtacaa acgggaccgg actacgctgg
     2881 gtaatttcgc gaacgtgagc gaaaaaggca tcgcccacaa aatcatgttc gctgggatgg
     2941 atggcaataa tcgccggagt atttgtcgct tctgcttcat ccacgactgc gcgaaggaaa
     3001 cagttatccg ccacattaaa tgcgccaatg gcaaacccgt cctggcggat aggttgcagt
     3061 aattctttca tcgaaactaa catgttaaat ctccagtttc agcgtgtagt ccgccctgcc
     3121 ggtgcgttat tgcactggca taaaggccat gacagaaaat gaatcaggct ttgccgatag
     3181 agccgctcgc ctgtaaatac ggtgtaatcg cttcttcgaa ggccagttcc atagctttca
     3241 gcgcgtccac cagttcaatg gatatatcgt gaccgagggt atgcaggag
//
LOCUS       DAWDRC010000458         3286 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11487_length_3286_cov_2.210771, whole genome shotgun sequence.
ACCESSION   DAWDRC010000458 DAWDRC010000000
VERSION     DAWDRC010000458.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11487_length_3286_cov_2.210771"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          744 a          935 c          808 g          799 t
ORIGIN      
        1 cgccaacgag aacggctggt tttgccgcca gaggatagcg tcgttattcg ccgtaagtac
       61 gcgtttgaaa tgctggacaa aatgcggcgt cgcaataggg tcagggcaat cactccagcg
      121 cagcgagtcg gcatcaggtt ggttttccca ctcatcccat acagggagta acaacaccag
      181 ccagcttccc gctttcaacg ttccgctaag tgccgcaaag gcagcggcat caaagccttg
      241 gcgggcgtcg aataccgcat gccggaactc gcgcccaagt aaagtttgta gcgccgaggg
      301 tgaacagtgg ttttcagcat ctggctgcgg cgaaatccac agccagtcgc caggtaaggc
      361 atcacgcaac ttaagcgcat gatcaaaaca ccaaccctct tccccgctca acaccagcaa
      421 gcggcggatc ccttcacgtt tcatttgcgc tgttaatgtg tgaagcgcag tcagttcagc
      481 catccctgcc ccggaagtta aatgctttta ccaaaagtat tgcattgtgc cggatcgccg
      541 ctgtcgaaac cacgtttaaa ccagctatag cgttgctgag aagtgccatg agtgaaactg
      601 tctggtacta ctcgcccctg actttgctgt tgtaaacggt catcgccgat ggcctgcgcc
      661 gcgttcagcg cctcttccag atcgccagtt tccagaacac cttgctgctg catactatgc
      721 ccccatacac cggcaaaaca gtcggcctgg agttccatac gcacagataa gcggtttact
      781 tccgcctgcg tcgcgttttg ttgcagttga cgaactttcg gctcgatgcc taacagtttc
      841 tgcacatgat gaccgacttc atgagcgata acgtacccct gggcaaaatc gccatccgcg
      901 ccaagtttgt ctttcatgtc atcatagaag gagagatcga tataaactgt gccatccgcc
      961 gggcaataga atggccccat aatggactgt cccgccccgc agccggtacg cgtcattcca
     1021 cggtacatga ccagcttcgg ttgctgatag gttttaccca tcttctcgaa ttgctgtccc
     1081 caggtgtctt ccgtggttgc cagaatcacc gaggtgaatt ttgcggcttc gtcttcatta
     1141 gggctaattg accgcgttga ttgctgttgg gaaaccggct gtccggtcat caacccggtt
     1201 aaatcaacgc catagtagcc tgcaaccagc acgactatca gtaaaatcag cccgccttta
     1261 ccgcttggca ggcgaaaacc gggaccgtcc atagacggac caccagagct gttgcgcctg
     1321 tcttcaacat tgtcactttc acgtcgccct tgccaacgca tagatacctc aacattatat
     1381 tcattataga aatgatcgta ggtggttcag aggaagatta ccacaggaaa gcgggatgaa
     1441 atggcggagg aatgagcgtt caatgcacat cttatgagag cggttgtctt gccgtgccag
     1501 cagcacggca agatgatgaa cagaaaaatc agtccagctg tacacccagg cggcgggcaa
     1561 cggcttcata ggcttcgatc aggccaccga ggctctgtcg gaaacggtct ttgtccattt
     1621 tctccagcgt ttctttgtcc cacaggcggc tgccgtccgg ggagaactca tcgcccagta
     1681 ccacttcacc tttgtacagg ccaaactcca gcttgaagtc gaccagaatg agaccagcat
     1741 catcgaacag ttttttcagc acgtcgttcg ctttgtaggt cagctctttc atacgcgcca
     1801 ggttctcttt gctcacccag ccaaaggttt cgcagtaaga ttcgttgacc atcggatcgt
     1861 gcatggcgtc gtttttcagg aacagatcga acagtggcgg gttcagctca ataccttctt
     1921 cgattccaag acgtttcacc agagagccag cagcacggtt acgcacgaca cactcaaccg
     1981 gcaccatatc cagctttttc accagacatt cggtatcgga gagcagacgc tccatttgag
     2041 tcgggatacc cgcttcagcc agtttgctca taatgaagta gttgaacttg ttgttcacca
     2101 tacctttgcg atcaaactgc tcaatacgcg cgccatcccc tgctgacgta tcattgcgga
     2161 attcgagcac caacaggtcc gggttttccg tgctgtatac ggttttcgct ttaccacgat
     2221 acaactcagc ttgcttttgc atctttatca ctcctgggtg tgaattaacg ttttaaaatc
     2281 ttttgctgtc tggtgtgccg gatgttttgt cggatgcggc gtgaacgcct tatccgacct
     2341 acgtccgaca aacaaatttc gtgcgaatta ccgctaaaat cgcgtttttc tgccgacgca
     2401 cacgtttgcg tatcatatca gaaaaaaggg ccggatgact ccagccctgt atttttactt
     2461 gctaaacgca gcctggaaga cagctaccag cgcgtcgttc tgactctgag tcagagtatg
     2521 acctttcgga tcgatgaact gtaggctgct gcggttatct aaatcgccaa cctgcagttt
     2581 atagtcaccg gatgccagac ctggatcgct cgcgcccagt tcgtgccatt cgctgtcaga
     2641 cagcggctta taagttacgg ccatgttgcc ctgcgaacgg gtgctgtcag tcactttcat
     2701 gcccactttt tccagcgcag ctggcagacg ttgccaaacc acattgaacg gcccgcgtac
     2761 gaccagcatt ggtaaaccgg tgtcatcagc tgcactttgt acgtccatag tggtggaggc
     2821 acgattttgc gcagcgttcg cggcgtcagt ggcagattta tccagaccgg cggaaataac
     2881 gttcatcatc tccgtgctgt aacgctgcat ggacgccgcg tctgcaaccg gtttgcccgc
     2941 ctgttccagg ttcagcagtt taaccgtaac cgcctgctga taaccctgcg gcttaacaga
     3001 gatttgataa cgaccacgat actgctcgtc ttcgtccaga cggttccatt gtacccaatc
     3061 ggtggtcagt gtctgaccag catcatcacg ttgggtgatg gtgtagtttt tcgcctgcag
     3121 cacgctaacc acctgcggcc acagagtatt gccacgacca ttttccacca gcaatgaagc
     3181 ggtatcgccc gtgaactggg tacgcgcgcc agaaaccagt gccagcggct gggctggtgg
     3241 acgaatgtcc agcgccttac cgacggcacc actaccgttg gtcacc
//
LOCUS       DAWDRC010000459         3284 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11494_length_3284_cov_2.043976, whole genome shotgun sequence.
ACCESSION   DAWDRC010000459 DAWDRC010000000
VERSION     DAWDRC010000459.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11494_length_3284_cov_2.043976"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    834..843
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          946 a          818 c          683 g          827 t
ORIGIN      
        1 gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac tcactaaaac cgccatcctg
       61 atgcacgcca atgactgaaa ttttttcaca gcaattggta cgaccgcttt tacacgccgg
      121 gcattgctga caagaaatat acggaattac cgcaacttgc tgtccgatat taaaattgtg
      181 aatgtttttt cctgattcaa taatctcccc acatatttca tggcctaaaa cgcgtggata
      241 actgaaaaat ggctgattgc ccgcccaggc atgaatatct gtaccgcaaa tcccgacagt
      301 tttaatttta attaatgctt cattatcgcc tggcaccggt ctttcccgga cttcccatac
      361 taaattcttt atttcctgac aaattaatac tttcattgtg gacatgattc accgctcctt
      421 ttattttatg actttcagat atcgtcgaat ttataagagc gcctgaattt acccataaaa
      481 tatgcggatg atcgcgcata aaaatgtttt aaatcggctt ttaatgccgg actggaggga
      541 agaatgagtc gttcgcagaa tttacgtcat agcgtcataa atcaggtgat tgaagatatg
      601 gcgaggggca atattccttc tccgctgcct tcacaaagcg gactggcaga aatgtataac
      661 atcagtcgca ctacggtacg ccacatactc cagcatttaa gcgcatgtgg cgtgctcacc
      721 ctggtgggga aaaattatgt tatcgcccgc aaacccgaac agaacgatgg atttgattgc
      781 atcacggcct cgctgaaaga gcaaaaccgt cttttcgagc aggcattttt cacnnnnnnn
      841 nnntgattaa cctgcgccag ttacgcgccg gagaaagctt ttccgaactt cagctggcgc
      901 gagcggcaag cgtcagtccg gtagtggtac gagaatatct tttgaaattc ggtcgttata
      961 atcttattca aagcgaaaag cgcggccaat ggagcatgaa gcagttcgat caatcctacg
     1021 ccgagcaatt gttcgaactg cgcgaaatgc tcgaaacaca ttccctgcaa cactttctca
     1081 accttcccga tgatgaccct cgctggctgc aagcaaaaac catgctcgaa cgccaccgga
     1141 cgctgcgcga cagcattggc agcaactttc gaatgttttc tcaattagac cgtgattttc
     1201 atgccatgct gttatccgct gctgacaaca tcttttttaa tcaatcactt gaaattatat
     1261 cggtgatctt tcattttcac tatcaatggg atgaaagcga tctcaaacag cgcaatatca
     1321 ttgctgttga tgaacatatg accattctca gcgccctgat ttgccgtagc gatttagatg
     1381 caataacggc gttgcgaaac catctggata ccgccaaaca atcaatgatt cgttccatca
     1441 ggcaacatca ccgataaatt aagtacagat taaaaacatt gattcttggt ttatatcact
     1501 atgttgtatt aatgcagtat taatccaggc gattgttctg cccataagga aatacatagg
     1561 gaataaacga tgctggaagt attactctca gccatcattt aattccctat tactggcagc
     1621 gataaaatta tcggctatcc atgattaacg tttccagcac atctaattca ctgactattt
     1681 ttgccagtgt ttcatcactg acttcttgcc ttgcgcgcat ctggtaaata gccgcgcgtt
     1741 tggcatttaa cgccgccaga ttaaatgttc tttccagctt cgcacgctgc atatgcaggg
     1801 aattatcatt aggcgacata tcctcttcac tatgtcgcat ataaccgata actctggccc
     1861 ccgtttccat aatcaactcg ttatcgaggt tttctgttct ggcgcgcagc aaacgttgct
     1921 cggtcttttg cacactcagt atggccattt tagccataaa cagatcggca gcttcctctt
     1981 ctttttgtcg gttatcgctg aaaatcccca catttttccc cagtaataac ggcagaataa
     2041 taatgccgca aaccagcgaa aagaggatca cacctgtggc gagaaatacc agcaagtaac
     2101 gcccgggaaa aggtgtgcca tccggcagca ataacgggat agaaagcacc cccgcaagcg
     2161 tgatagctcc gcgcaccccc gcaaaggtcg ccagcagcat atgacgcgtt ttatatccag
     2221 caaacagcat cggatgtttg ccgggtcgtt tacggctata cagacgcatc agccaaagcc
     2281 agcccaggcg cacaaccatt aacgccaggt agatccaaat gacatcaagg agcagcgatc
     2341 cggtgtccac cagtggatcg ttttctgcac ggatcagtga ttccgaaata atgcccggca
     2401 gttgtaagcc taaaatcaga aacaccatgc cgttaaagac atattcaagc atcgaccagc
     2461 tacttttagc tttaattctc aggcgaatag gcgcgcggtg gactacgcca atgcgactgg
     2521 ttgttaaccc cgcggcgaca gcggcaagaa tgccagataa gccaaaatgt tccgcaacca
     2581 gataaacaat aaacggcaac agaagcatca atacaatctg aatggaaggt tcatcgtgag
     2641 catatcggct gatcaacagc agccctttac catataacca ggtaacagcg atccccacgg
     2701 caataccgcc caacgcaacg acaaaaaacg caatggttgc gccatgaacg ctgaattcca
     2761 tcgttcccat cgtcactgca acagcaaatt tcaacgccac caggccggaa gcatcattca
     2821 tcaacgcttc gccttcgagc acttcctgga tgtttttgct gatacgccct ttcccgacaa
     2881 tccccagcaa cgccacggca tcggtaggcg acaacacggc ggctaatgcg aaagcagcga
     2941 tgagcggcac attcggtaag agtaaatgaa tcacatagcc gacaccaaaa atagtgatta
     3001 gtaccaacac aagtgccagg ctgataattt cccggctgtg cttgatcaat tctttaggtt
     3061 gtatttttga actttcagca aacaacaggg gtggtataaa caataagaga aaaagttcag
     3121 gattgaattc cacatgcaga ccaaatatag gctgggccag tactgcgcca gcggcaatct
     3181 ggattaaagg aagtggtatc tggacaggag acagacgtgt aagcacgctg gttaatgata
     3241 ctaccaacgt aagaattaaa attgttaaaa gtatttccat ataa
//
LOCUS       DAWDRC010000460         3282 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11508_length_3282_cov_2.199566, whole genome shotgun sequence.
ACCESSION   DAWDRC010000460 DAWDRC010000000
VERSION     DAWDRC010000460.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11508_length_3282_cov_2.199566"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    942..951
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          735 a          843 c          946 g          748 t
ORIGIN      
        1 ccgctgatga agtgtggggt tacctgccgg gcgaacgtga aaaatacgtc ttcaccggcg
       61 agtggtacga aggcctgttt ggtctggcag acagtgaagc aatgaacgat gcgttctggg
      121 acgagctgtt gaaagtgcgt ggcgaagtga acaaagtcat tgagcaagcg cgtgccgata
      181 agaaagtggg cggctcgctg gaagcggcag taaccttgta tgcagaaccg gaactggcgg
      241 cgaaactgac cgcgctgggc gatgaattac gatttgtcct gttgacctcc ggcgctaccg
      301 ttgcagacta taacgacgca cctgctgacg cccagcagag cgaagtcctc aaagggctga
      361 aagtcgcgtt gagtaaagcc gaaggtgaga agtgccctcg ctgctggcac tacacccagg
      421 atgtcggcaa ggtggcggaa cacgcagaaa tctgcggccg ctgtgtcagc aacgtcgccg
      481 gtgacggtga aaaacgtaag tttgcctgat gagtcaatcg atctgttcaa cagggctacg
      541 ctggctgtgg ctggtggtag tcgtgctgat tatcgatctg ggcagcaaat acctgatcct
      601 ccagaacttt gctctggggg atacggtccc gctgttcccg tcgcttaatc tgcattatgc
      661 gcgtaactat ggcgcagcgt ttagtttcct tgccgatagc ggcggctggc agcgttggtt
      721 ctttgccggt attgcgattg gtattagcgt gatcctggca gtgatgatgt atcgctcgaa
      781 ggccacgcag aagctaaaca atatcgctta cgcgctgatt attggcggcg cactgggcaa
      841 cctgttcgac cgcctgtggc acggcttcgt tgtcgatatg atcgacttct atgtcggcga
      901 ctggcacttc gccaccttca accttgccga tactgccctt tnnnnnnnnn nttgtgtcgg
      961 tgcggcactg attgtgctgg aaggtttttt accttctaaa gcgaaaaaac aataataaac
     1021 cctgccggat gcgatgctga cgcatcttat ccggcctaca gattgctgcg aaatcgtagg
     1081 tcgcatccgg caaaaatcct caaaatataa gagcaaacct gcatgtctga atctgtacag
     1141 agcaatagcg ccgtcctggt gcacttcacg ctaaaactcg acgatggcac caccgctgag
     1201 tctacccgca acaacggtaa accggcgctg ttccgcctgg gtgatgcttc tctttctgaa
     1261 gggctggagc aacacctgct ggggctgaaa gtgggcgata aaaccacctt ctcgctggag
     1321 ccagatgcgg cgtttggcgt gccgtcaccg gacctgattc agtacttctc ccgccgtgaa
     1381 tttatggatg caggcgagcc agaaattggc gcaatcatgc tttttaccgc aatggatggc
     1441 agtgagatgc ctggcgtgat ccgcgaaatt aacggcgact ccattaccgt tgatttcaac
     1501 catccgctgg ccgggcagac cgttcatttt gatattgaag tgctggaaat cgatccggca
     1561 ctggaggcgt aacatgcaga tcctgttggc caacccgcgt ggtttttgtg ccggggtaga
     1621 ccgcgctatc agcattgttg aaaacgcgct ggccatttac ggcgcaccga tatatgtccg
     1681 tcacgaagtg gtgcataacc gctacgtggt cgatagcctg cgcgagcgag gggctatctt
     1741 tattgagcag attagcgaag taccggacgg cgcgatcctg attttctccg cacacggtgt
     1801 ttctcaggca gtacgtaacg aagcgaaaag ccgcgatttg acggtgtttg atgccacctg
     1861 tccgctggtg accaaagtgc atatggaagt cgcccgcgcc agccgtcgtg gtgaagaatc
     1921 tattctcatc ggtcacgccg ggcacccgga agtggaaggg acgatgggcc agtacagcaa
     1981 cccggaaggg ggaatgtatc tggtcgaatc gccagacgat gtgtggaaac tgacggtcaa
     2041 aaatgaagag aagctctcct ttatgaccca gaccacgctg tcggtggatg acacgtctga
     2101 tgtgatcgac gcgctgcgta aacgcttccc gaaaattgtc ggtccgcgca aagatgacat
     2161 ctgctacgcc acgactaacc gtcaggaagc ggtacgcgcc ctggcagagc aggcggaagt
     2221 ggtgctggtg gtcggttcga aaaactcctc caactccaac cgtctggcgg aactggccca
     2281 gcgcatgggc aaacgcgcgt ttttgattga cgatgcgaaa gatatccagg aagagtgggt
     2341 gaaagaggtt aaatgcgtcg gcgtgactgc gggcgcatcg gctccggata ttctggtgca
     2401 gaatgtggtg gcacgtttgc agcagctggg cggtggtgaa gccattccgc tggaaggccg
     2461 tgaagaaaac attgttttcg aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga
     2521 ttaagtcatt agtagtctaa gttatgcgaa aatgccggtc ttgttaccgg cattttttat
     2581 ggagaaaaca tgcgtttacc tatcttcctc gatacagacc ccggcattga cgatgccgtc
     2641 gccattgcag ccgcgatttt tgctcccgaa ctcgacctgc aactgatgac taccgtcgcg
     2701 ggtaatgtct cggtagagaa aaccacccgc aacgccctgc aactgctgca tttctggaat
     2761 gcggagattc cgcttgccca gggcgcagct gtgccgctgg tacgcgcacc gcgtgatgcg
     2821 gcatctgtac acggtgaatc gggaatggca ggctacgact ttgttgaaca caaccgtcag
     2881 ccactgggta taccggcgtt tctggcaatt cgtgatgccc tgatgcgtgc gccagagccc
     2941 gttaccctgg tggccatcgg cccgttaacc aatattgcgc tgttactttc acaatgcccg
     3001 gaatgtaagc cgtatattcg ccgtctggtg atcatgggcg gttctgctgg tcgcggcaac
     3061 tgcacaccaa acgccgagtt taatatcgct gccgatccag aagcggcagc ctgtgtcttc
     3121 cgtagcggca tcgaaatcgt catgtgcggt ctggatgtca ccaatcaggc aatattaacc
     3181 cctgactatc tcgctacttt gccggaattg aatcgcaccg gaaaaatgct acacgccctg
     3241 tttagccact accgtagcgg cagtatgcaa agtggactac gt
//
LOCUS       DAWDRC010000461         3269 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11565_length_3269_cov_3.011201, whole genome shotgun sequence.
ACCESSION   DAWDRC010000461 DAWDRC010000000
VERSION     DAWDRC010000461.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11565_length_3269_cov_3.011201"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          835 a          785 c          789 g          860 t
ORIGIN      
        1 gttggtgcgg aataaatgcc ggatgcgacg ctggcgcgtt tgctccagcc tactcttgct
       61 tatccgtcct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta acgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc cggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaaatgtag atccagctct tcttcgtctg gtgtttccgt catcaaggcc
      361 catcgctgtt ggctggcaac gatggtttgc gccgcgccag ccttgcgcag ctcataacta
      421 tctttgcccg gtttatcaac atccatatca tggtgcgtat gcttaatcag ccccggacgg
      481 atccctctgg cgcataatgc cgggatcaat tttttcagca gcgttgtttt tccggtgcca
      541 ctccatgcag caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgtttata aatgcctctt tacgatcgct gaaatcaacc gcatgaccgc
      661 cagccagacg cataaatgcc ataacccggc gttctcctgc ttgcagatat tccagtaata
      721 aaggctcaat tgcgcggttt accagagcaa tagtcgggtg atcgcgttca ccgtcgtgga
      781 cccacacgac aggcgcatct ttgcgctgat gagtaagccg ggcggctaaa tcatggggaa
      841 tgtaaggcgt atcgcacggg caaaacaaaa accactcacc cgcttcctgc tgcattactg
      901 aaagcattcc tgccagagga ccagggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttacctg atagatttcc tgatgacgat tagcattaac cacgacgtga gaaagctgcg
     1021 tcataagcgc gtcagcgaca tgctgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcccgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatta ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgctg caattcttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagatttc aaaacggcgc tattacgcgc acgtggtgta attaaagagt aaaagcttgt
     1501 aagcggcgca accaaaatca tcgtgaaatg atatccttca tcattcgtaa tgttttccgg
     1561 atgatgggat gaacaacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcatggg attcgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag atcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 ttaacgatga agttccggtc gcagcacctg tggcctttaa tggtcagact ttattgaacc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gatggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcgca
     1981 aacagctttt tatccatcgc ccgaccatcg gtttgaatga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctaatacctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gattgccgcc gttacagcac actggcggga agatttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagccgttc aggatttgtg gatgttgctc aatggtgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaggc ttatgaagaa tttagcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggcta cgacagacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttgcaatta acacctatgt attcatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccagttgctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgccc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aacgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 gggggtgacc tgggcaaaga gctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tcacagttcc gctgtttgaa ggcgtacaaa aaacccagac cattcgttca
     2941 gcatctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt gaaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggtgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggtatggata ccagcaatat ggatgttttt gttcagcagt atgctgatac tgtgaaatat
     3181 ctgtccgaga aaaaataatt caatgtaaat tacataaagc ccgtgaatat tcacgggctt
     3241 tttttattat ttaataaata taaatacat
//
LOCUS       DAWDRC010000462         3267 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11574_length_3267_cov_2.734745, whole genome shotgun sequence.
ACCESSION   DAWDRC010000462 DAWDRC010000000
VERSION     DAWDRC010000462.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11574_length_3267_cov_2.734745"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          778 a          819 c          806 g          864 t
ORIGIN      
        1 gtaacgagct gcagcgcagt gatgacatca ctctgaatct cgaccaccag ctgcttggcc
       61 tcggctccaa ctcctggggc agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact
      121 tcagctacgg ctttacgttg ctgccggttt ctggcggaga agctaccgcg caaagcctgg
      181 cgtcgtatga gttcggcgca gggttctttt ccacgaattt gcacagcgag aataagcaat
      241 gaggatcatc gataacttag aacagttccg ccagatttat gcctctggca agaagtggca
      301 acgatgcgtt gaagcgattg aaaatatcga caacattcag cctggcgtcg cccactccat
      361 cggtgactca ttgacttacc gcgtggagac agactccgcg accgatgcgc tatttaccgg
      421 gcatcgacgc tattttgaag tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc
      481 gccgaaagag acattacagg tagtggaata ttatcgtgat gaaactgacc gtgaatattt
      541 aaaaggctgc ggagaaaccg tagaggtcca cgaagggcaa atcgttattt gcgatatcca
      601 tgaagcgtat cggtttatct gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat
      661 cgaagatggt tatttccata acaaataaca actacggcgg caaaaggagt ttgccgccac
      721 cgctacccta ctcattttcg gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc
      781 aaattcggct tgctctcgct gacttttgcc gccgtttaca gctttaacaa cgttatcaat
      841 aataatattg agcttggact ggcctcggca ccgatgtttt tcctcgcaac aattttttat
      901 tttattccct tctgtctgat catcgcagaa tttgtttcgt taaataaaaa ctcggaagcc
      961 ggtgtctacg cgtgggtaaa aagttcgctg ggcggacgtt gggcatttat tactgcctat
     1021 acctactggt tcgtaaacct gttctttttc acctcgttgt tgccgcgcgt tattgcttat
     1081 gcttcgtatg ccttcctcgg ctacgaatat attatgacgc cggttgccac caccattatc
     1141 agtatggtgc tgttcgcctt ctccacctgg gtttccacca acggggcgaa aatgctgggg
     1201 ccaatcacct ccgtcacttc aacgctgatg ctgctgttaa cgctctccta cattttactg
     1261 gcaggtacgg cactggttgg cggcgtacag cctgccgatc cgatcaccgt tgacgcgatg
     1321 atcccgaact tcaactgggc gttcctcggc gtgaccacct ggatctttat ggccgcaggt
     1381 ggcgcggagt ccgtcgctgt gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt
     1441 aaagtgatca tcctcgccgg gatttttatc ggcgtactgt attccgtctc ctcggtgctg
     1501 attaacgtct tcgtcagcag caaagagttg aaatttactg gcggatcggt acaggtattc
     1561 cacggcatgg cggcgtattt tggtctaccg gaagcactga tgaatcgctt tgtcggtctg
     1621 gtgtccttta ccgcgatgtt cggttccctg ctgatgtgga cggcaacgcc ggtgaaaatt
     1681 ttcttctccg aaattccgga aggcatcttt ggtaagaaaa ccgtcgaact gaacgaaaac
     1741 ggcgttccgg cgcgcgcagc gtggatccag ttcctgatcg tcatcccgct gatgattatc
     1801 ccgatgctcg gttccaatac cgtgcaggat ctgatgaata ccattattaa tatgaccgcc
     1861 gcagcgtcca tgcttccgcc gttattcatc atgctggctt acctgaattt acgtgccaaa
     1921 ttagatcacc tgccacgcga tttccgtatg ggttcccgac gcaccggtat tatcgttgtt
     1981 tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg
     2041 aatattctga ccatcatttt ttataacgtc ggcggtattg ttatcttcct cggctttgcg
     2101 tggtggaaat acagtaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg
     2161 acgccagcca gcaatgttga ttaagcagaa taataaaaaa tgagcaggca ttttccctct
     2221 tcctggatgg ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa
     2281 aaggctgtac tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg
     2341 cagagcatta cttgctctgc cgttttgcta cgccttttct gcggccgctg aagaagcacg
     2401 tccggcagaa catgacgact caaaaacccc cgcaatgacc tcgacatctt ctccttcatt
     2461 tcgtttttac ggcgaattag gggttggtgg atatatggat ttagagggtg agaataaaca
     2521 taaatacagc gacggcacct atattgaagg tggcctggag atgaagtacg gctcctggtt
     2581 cggcctgatt tacggcgaag gctggaccgt acaggccgac cacgacggta atgcctgggt
     2641 gcctgaccat agctggggtg gcttcgaagg tgggattaac cgtttctatg gcggttatcg
     2701 taccaatgat ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc tggatgacct
     2761 gcaatggtgg ggcgatttca cgcccgatct aggctacgtc attcccaata cccgcgatat
     2821 tatgactgcg ctgaaggtac agaacttaag cggcaacttt cgttatagcg ttaccgccac
     2881 acctgccgga catcatgacg aaagcaaagc ctggctacat tttggcaagt acgatcgcta
     2941 cgacgacaaa tatacctatc cggcaatgat gaacggttac atccagtatg accttacaga
     3001 aggtatcacc tggatgaatg gtctggaaat caccgacggc acaggccaac tctatctcac
     3061 gggcctgctt acccctaact ttgccgctcg cgcctagcac cataccggac gcgccgacgg
     3121 gctggacgta tcgggaagtg aaagcgggat gatggtgagc gccatgtatg aagcgttaaa
     3181 gggcatttat ctctccaccg cttacaccta cgccaaacat cgccctgacc acgctgacga
     3241 tgaaaccacc tctttcatgc agtttgg
//
LOCUS       DAWDRC010000463         3267 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11578_length_3267_cov_2.023661, whole genome shotgun sequence.
ACCESSION   DAWDRC010000463 DAWDRC010000000
VERSION     DAWDRC010000463.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11578_length_3267_cov_2.023661"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2106..2115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          861 a          842 c          819 g          735 t
ORIGIN      
        1 ttccaggaaa cggagggtga aatacagcac catcatcgat gcgttttcaa tggcgatatc
       61 gcgaatatca ccttcaataa cgtctactgg cgtaggagct ttataggcgt caatatgacg
      121 acggcagcgt tcaatcatcg ccggggagtt atcgatggca ataattttgc aattatcatg
      181 atgaatgttg cgacgcaccg agagcgtcgc cgcgcccaga gaacacccca gatcgtaaac
      241 ctgcgtacca ggttgaacga agcgctcggc taacatacca atcatggaaa taatattgga
      301 atagccggga acggaacgct ggatcatatc cgggaagact tcagctaccc gttcatcaaa
      361 ggtccagtcg cccagtctgg cgataggggc agaaaatagc gtgtcgcggt gagacataac
      421 gtaaaaatcc gggaaaaaga aagtggcgta ttgtgcgcta acgcagggag aaaaccaact
      481 cccagggcat ataccaaaga ttcgccagca ccatcagcaa cagcgcacac caggtggcgc
      541 tcatgccaga acgtcgccag cggaacagac ggtgatgaaa accgtaataa tgcatcagac
      601 gaccagcaag caaaacgatg ccgcaaatat gcaccatcca ggtttctgcg ccattcattt
      661 ccataaacag catcaacaca atcgcgatag gaatatattc caccgcgtta ccatgaatgc
      721 gaatagcgct ttgcagttcg ctaaaaccgc cgtcgccata ggcaacgcgg tactgcattc
      781 gcaggcgaac gacatcaaaa gagaacttca ttaataacaa cgcacttaaa acggcataca
      841 gcgcgcttac catacaaact ccctttaaaa tggccgatgg cacctgtcta tgataggtgg
      901 caaattcaga aaagagaaga ttgctgtgga atagccggaa ctgctccgat ctctggaagc
      961 gttttacgta agtcttccca cagggtatgt accagttccg gggcctggac aatatctggc
     1021 gtatgtaaaa aaagataagg cgtagtggtc tgatgccact gcgctaattt ttgtaaccag
     1081 acctgaaata attcccggtt ttgcgtcata tcatcactac cgataaaacg gatcagtgga
     1141 tttttcgccg tcagtacagc atgtaccggc actttaggtt tttttcgttg agcgtcgcga
     1201 atagcttcac tgtgtggacg tgctgcatga accgggcggc tgtctaaaat cacccgatta
     1261 acgccgcgct gatgtaaacc gcgattaagc gtttgttcct cctccccttt ggcgaaaaac
     1321 tgtggatggc ggacttccac accataatta aattcgccgg gaagagaatc gagaaaatgc
     1381 caaagcgcag gcagctcccg tgggccgaat gtggcaggca gttgcagcca gtattgtcca
     1441 atgtgcggag ccaacggtga catgcgggtc aaaaattcag tcactaaatc atcgcaatgc
     1501 cgtaatgctg cctgatgcga aatagtcgcc ggaaacttaa aacagaagcg gaagtcatct
     1561 gtggtctgct cacgccagcg caggacaacc tcgggtttcg gcagggcata aagcgtggtg
     1621 ttaccctcca cacagttaaa gtggcgggca tactcttcaa ggctggtgat ccccaaccgc
     1681 acccatttag gatgcgacca ttgtggcaaa ccgatgtaaa tcataacgcg ttgaggatct
     1741 cttcaacgct acgcacacgg gcgatgcgcg ggtagatatg attaatgctg ttattgtgct
     1801 gctcggcgct agcggcgcta caggcatctt cggcaatcac cagattaaaa ccgagttccc
     1861 aggcattgcg ggcggtggat tcaacaccga tattggtcga gatcccacat aacactattg
     1921 tatcgatacc ccggcggcgt aattgcaact ccagatccgt accgtaaaac gcaccccatt
     1981 gacgtttgat gatttcgata tcgctgtcgg ttgcacctaa tgcagcagga tgttgccacc
     2041 agttttcggg caacactttt gcgggggagg gggcatcaac cgactgtttt aatgcttcgg
     2101 cgtaannnnn nnnnnttcag cataatcggc agaccagcca acacgcacca gaaacacggg
     2161 ctgaccgctc gcgcgaaatt tcgccgccag cttcccggcg cgattaacca cctcatcggc
     2221 agtatatgga cctccggcaa aaggtaagat gccttcttgt aaatcaatca ccaccagcgc
     2281 ggtggtttta gcattaagtt caagcatgat gactcctgat aaaacaaaat cgtgcctctc
     2341 accttaatgc gattagccga tggcggaacg acaaattttg ttaattttta tgagttcggg
     2401 caacaccttt atagcaaact ggcgtagagt ctgatttcag gcccgatata aagtggtaac
     2461 gaggcgttcc agcgcgggcc aggctcttac ggtgcggcag aatttccagt ataatagccg
     2521 ccttttttca ttcagttgtg acatacagct aacgctgcga cttggtcacc tgcgcagcag
     2581 ggggcatccg ccggcagcta agttaaggga tatctcatgc gtacagaata ttgtggacag
     2641 ctccgtttgt cccacgtggg gcagcaggtg actctgtgtg gttgggtcaa ccgtcgtcgt
     2701 gatcttggta gcctgatctt catcgatatg cgcgaccgcg aaggtatcgt gcaggtattt
     2761 ttcgatccgg atcgtgcgga cgcgttaaag ctggcctctg aactgcgtaa tgagttctgc
     2821 attcaggtca cgggcaccgt acgtgcgcgt gacgaaaaaa atattaaccg cgatatggcg
     2881 actggcgaaa tcgaagtact ggcgtcctcg ctgactatca tcaaccgtgc agatgttctg
     2941 ccgcttgact ctaaccacgt caacaccgaa gaagcgcgtc tgaaataccg ctacctcgac
     3001 ctgcgtcgcc cggaaatggc tcagcgcctg aaaacccgcg ctaaaatcac cagcctggtg
     3061 cgccgtttta tggatgacca cggctttctc gacatcgaaa ctccgatgct gaccaaagcc
     3121 acgcctgaag gcgcgcgtga ctacctggtg ccttctcgtg tgcacaaagg taaattctac
     3181 gcgctgccgc aatccccgca gttgttcaaa cagctactga tgatgtccgg atttgaccgc
     3241 tactatcaga tcgttaaatg cttccgt
//
LOCUS       DAWDRC010000464         3248 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11658_length_3248_cov_2.348888, whole genome shotgun sequence.
ACCESSION   DAWDRC010000464 DAWDRC010000000
VERSION     DAWDRC010000464.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11658_length_3248_cov_2.348888"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          793 a          797 c          883 g          775 t
ORIGIN      
        1 acataacgga ctaccggagc gggacaacca tggaacgacg tggatttatt gtaatcccca
       61 tgaccgagtt atggggtctt cccccctgcg cagtattggc tggcaggggc tgccggatac
      121 taaagatggc acaccacata ccttatttaa acaggctgga gatacgcttt atgttcgtat
      181 acttgggcgc aatacaccct gcggcggtac gccgaccgca cagacccatt tcagtaattt
      241 gggtgatggc aaacccttct gggacagcac aacaacgctg ttacagaggg caacgtggcc
      301 ggatccggac agcgggcaga cattaactat taacgcgccg caggtgccgg aaccgctgac
      361 agctgaagaa ctaaagaatt ttgatcaaga ctatgcccgt gacgaaaagc aaagcggcgg
      421 agcaggatat gcttacgggc agattaaccc agagacgaaa aaacctgtcg ataccgacta
      481 tcgttattac atttcactgt atggctattt tgaccggaag atggtgccga aaaaagactc
      541 gggatactac cagtcagggc cggggtcgaa agaggacagg gtaaagtatg aaaagcagtc
      601 acaagaagag atgctggagg aggtaagaac ctatgttcag cgtccaacag atcacagtac
      661 cctgccatcg gatgagcgat ttatgagccg ggtggaaagc gatgactatt actttagtgg
      721 caagctgact gttccgccaa taccgcctgc gataaaacag gatgtcgccg aagatgctga
      781 acagagaaag gcagaagaaa aggcccggtt acgtaatgtg tgaaacaaat acatggaaag
      841 tgatatggga aaaaatgtgg tagtactcaa tgacgcaact tctcacggtg gcaaagtctc
      901 ttcggcctct tcaacctttg atattcaggg aaaaaaggct gcactgctga atgataccgt
      961 cacttgcccg aagcatggta ctaacccaat tgtggagtgt gataccagtt atgaggaggg
     1021 tggacgtggg attgttgtgc atggctgtaa aagcgcctgc ggctcggtgg tgtatgccag
     1081 tctgccggat gtggagatag gctgatgggg tggtcaaaac cgaaaattcc caccagcaaa
     1141 gacgtgcagc gctggtctcc ttgggtatgc ctttttattc tgtttgccgg ggcatttgtc
     1201 gcgctcacat atgggatact gcattcccct gcggacggac tccccgccct cgccagcggt
     1261 tactggttgc ccttgacagg caggatgatg gctggagtga tgacagccct aaccggctat
     1321 ttgttgtggt gggagtttca ggcatattac gtctggtgct ggaatagtcg gcggcacagc
     1381 gcactccttg tctggcagca gcgagcacat cagcaccgct acttagcctg ccatgttctg
     1441 ctgacctcag acacgcaact gcttcctcgc ctcgcaggta cttcttatga agataataat
     1501 aatgatcctc ctttaacatt attggcagag gagccgctga tacccggtat tgcgcgtttt
     1561 gagcaacttt gccagttact gataaaccag attaaagctt ctcttattca gcattatcct
     1621 tccggtcaga tgactgtact gattgaaacc tctgcatcgg ataaagagcg tgagcgaaca
     1681 acccttattc gtttatggaa agaggatgaa ttaccctgga tactggacat ccatatgtca
     1741 cccactcccc ttcctctcag tgatgagaat aaccacttat ttgatattga taaccctgtt
     1801 cttgttttgg cgttgcacta ccgccagccg gaggaaaacc tgccggaatt cgccagcgca
     1861 ttgttactgt tgccgcccga gttactgaaa gtttctatac ggcgggatac ggtgcgggtg
     1921 tttcgcccga tgccactaaa cattgatgcg atctcccgtg agttgactga actgcgggat
     1981 atggcgcaac agccagtgaa tgccagatat ctggtctggc acagtggtct gacacaggca
     2041 tcacaacagg ctctgggccg tgtggtgaac gaccttccgt tatccctgca taacgatatc
     2101 gccactggcg gaattattga tgtggataaa gttatcgccg cttacgggcc tcctgccggt
     2161 tggctgatgg tggcaaccgc cgcagagatg gtcagatacg ggccgggaag tcactggttg
     2221 ttacaggtca gaaataaaca ggccagtgct gtggtgacgg gcaacggcta tccggcgatt
     2281 aatcaggaat accttgatac aacctcatct ccttatcccg caggttccct aatgctggcg
     2341 gttatgctca atatactggt gctctggttt atcgggcggt acagccagga atggctgttt
     2401 tcctgggcag gcgtagccat gacactgttg ctgatgatag tgacattgcc aggcatcgtc
     2461 tttggtttac gaagcgctgt tgctcatctg caataccctc gctttataca ggctatcgtt
     2521 cgcgcccaga aggaataatt tgttatgaaa agagttgtgc gtcttttggg tgtggggtta
     2581 ctgctccttg ttgtgttgtt gctcattttg tttgttctgg ctcagaccac accgctgata
     2641 tcagcacagg atgagcatgc tgtctggctt cgtctgttga taacagcgat tgtgatctgt
     2701 ttgctaagta tgtgcatatt tttcctcttt tctttccggc agaacgaagc ctcgacgata
     2761 tcactatacg ctcaaccgac tgatataaag gaaataaata cggagcagcc gaactatgca
     2821 tcactgctga cgatatattt acgcgaccgc tacggtccgt tctggcggcg taaagtccgc
     2881 ctgctgctgg tgaccggcga gcctgaacag gcagaagcca tcgcgccggg gctgaccggg
     2941 caacactggc tggaaggcga ccacacggtg ctgatatatg gcggcaggcc aacagcggag
     3001 cctgatgtca cactgctgac cgccttaaaa aaactgcgcc gcagccgtcc gctggacggc
     3061 atcatctggg cgctgacaga agaacagagc cgccagacag cgcaactcga caaaggctgg
     3121 cgcggactga taaacggcga taagcgactc ggttttcagg ctccactcta tttgtggcag
     3181 gtctgtgacg acggtgatta tcagaccgga cgccccctgc aaagcgtcgg ctgcctgctg
     3241 ccggaacg
//
LOCUS       DAWDRC010000465         3248 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11657_length_3248_cov_2.361102, whole genome shotgun sequence.
ACCESSION   DAWDRC010000465 DAWDRC010000000
VERSION     DAWDRC010000465.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11657_length_3248_cov_2.361102"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          901 a          616 c          822 g          909 t
ORIGIN      
        1 actgggaagc atagtgttta ttacggcatt tctgatgttc attattgttg gtacctatag
       61 ccgccgtgtt aatgtcagtg gtgaggtcac aacctggcca agagctgtca atatatattc
      121 aggtgtacag ggatttgttg tcaggcagtt tgttcatgaa ggacagttga taaaaaaagg
      181 ggatcctgtt tatctgattg acatcagtaa aagtacacgt agtggtattg tcactgataa
      241 tcatcgtcgg gatatagaaa accagctggt tcgtgtggac aagattattt cccgtctgga
      301 agaaagtaaa aaaataacgc tggataccct ggaaaaacaa cgcctgcaat acacagatgc
      361 gtttcgtcgc tcatcagata ttatacagcg tgcagaggaa gggataaaaa taatgaaaaa
      421 caacatggag aattacagaa actatcagac aaaagggctg attaataaag atcagttaac
      481 taaccaggta cgaggtacag aaagaactgg ttaacactga tgtggagggc gaaatcatta
      541 tccgggcgtt gactgacggg aaagttgact ccctgagtgt cactgtcggg caaatggtca
      601 atcccggaga caaccttctg caggttattc ctgagaacat tgaaaattat tatcttattc
      661 tctgggtccc gaatgatgct gttccttata tttcggctgg tgacaaagtg aatattcgtt
      721 atgaagcctt tccggcagaa aaatttgggc agttttctgc tacggttaaa actatatcca
      781 ggactcctgc atcaacacag gaaatgttga cctataaggg agcaccacag aatacgccgg
      841 gcgcctctgt tccctggtat aaagtcattg cgatgcctga aaagcagatt atcagatatg
      901 acgaaaaaaa cctccctctg gaaaatggaa tgaaagccga aagtacacta tttctggaaa
      961 aaaggcgtat ttaccagtgg atgctttctc ctttctatga catgaaacac agtgcaacag
     1021 gaccgctcaa tgactaacgg gaatttcaga caaattataa atcagcttga tatgcgctgg
     1081 cgacgtcgtg ttccggttat tcatcagacg gagaccgctg aatgtggact ggcctgcctg
     1141 gcaatgatat gcggtcattt tggtaagaat attgacctga tatctcttcg tcgtaagttt
     1201 aatctctcgg cccgtggagc aaaccttgca ggaatcaatg gaatagcgga gcagctgggg
     1261 atgatcaccc gggctctttc actggagctg gatgaacttg gtgccctcaa aatgccgtgt
     1321 attctccact gggatttcag tcacttcgtc gtgctggtca gcgtaaagcg taaccgttat
     1381 gtactgcatg atccggccag aggaagaaga tatctcggtc gggaggaaat gagccggtat
     1441 tttacgggca ttgcacttga ggtctggccc ggaggtgaat tccggacgga aacccagcaa
     1501 acccgcataa gtctccgttc actgattaac agtatttacg gtattaaaag aacactggcg
     1561 aaaattttct gtctgtcagc tgtaattgaa gcaatcaatc tggtaatgcc ggtgggaact
     1621 cagctggtta tggatcatgc gattccggcg ggggaccgag ggctgctgac gcttatttct
     1681 gctggcctga tgttctttat attgctcagg gccgcggtga gtatgctgcg tgcatggtcc
     1741 tcactggtta tgagcacgct catcaatata cagtggcagt cgggtctgtt taaccacctt
     1801 ctcagactgc cgctggcatt ttttgaacgc cgtaaattag gtgatatcca gtcgcgtttt
     1861 ggctcccttg acactttgag ggccaccttt accacctgtg tggttggggc aatcatggac
     1921 agtattatgg ttgtgggtgt ttttgtgatg atgctgttat atggaggata tcttacctgg
     1981 atagttctcg gttttaccat ggtttacgtt cttattcgtc tggtgacata cggctattac
     2041 cggcaaatat cggaagaaac tcttgtcagg ggggcccggg ccagctccta ttttatggaa
     2101 agcctgtatg gtattgccac ggtaaaaatc cagggtatgg tcgggatccg gggaacacac
     2161 tggcttaacc tgaaaataga tgcgatcaat tcaggtatta agttaaccag gatggatttg
     2221 ctcttcgggg gaataaatac ttttgttgcc gcctgtgatc aggtggcgat tttatggctg
     2281 ggtgcaagcc ttgtgatcga taatcagatg acaataggga tgtttgtggc atttggttct
     2341 tttcgtgggc agttttcgga tcgggttgct tcgctgacca gttttcttct tcaactgaga
     2401 ataatgagtc tgcataatga gcgcattgca gatattgcac tacatgaaaa ggaagaaaag
     2461 aaaccggaaa ttgaaatcgt tgctgacatg agcccggttt cactggaaac cactgattta
     2521 agctaccggt atgacagcca gtcagcacag gtattcagtg gtctgaattt gtctgtggct
     2581 ccgggagaaa gtgtggctat aactggtgcc tccggtgccg gaaaaaccac attaatgaaa
     2641 gtattatgtg gactgtttga accagatagt ggaaaagtac tggttaatgg cacggatata
     2701 cgtcaacttg gaataaataa ttatcaccgt atgatagcct gtgttatgca ggacgaccgg
     2761 ctattttcag gatcaattcg tgaaaatatc tgtgggtttg cagaagaaac agacgacgaa
     2821 tggatgacag aatgtgccag agcaagtcat attcatgatg tgataatgaa aatgccaatg
     2881 gggtatgaaa cgttaatagg tgaactgggg gaaggtcttt ccggcggtca aaaacagcgt
     2941 atattcattg cccgagcttt ataccggaaa cctggaatat tatttatgga tgaggctaca
     3001 agttctcttg atacagaaag tgaacgtttc gttaatgctg ccataaaaaa aatgaatatc
     3061 acccgggtga ttattgcaca cagagaaact acgttgagaa ctgttgacag gattatttct
     3121 atttaaaatc cactggtgta actttgtaag gagttttgtc gatgggggag gtcaagaagg
     3181 atataaaaat aacagtgatt gcttttgtta tcaattatct gttcttttat attccggtgt
     3241 cattatat
//
LOCUS       DAWDRC010000466         3247 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11664_length_3247_cov_2.611216, whole genome shotgun sequence.
ACCESSION   DAWDRC010000466 DAWDRC010000000
VERSION     DAWDRC010000466.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11664_length_3247_cov_2.611216"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          735 a          836 c          838 g          838 t
ORIGIN      
        1 ggtgctggat atgctggagg cgcatagcga cagttccatt gttccgttaa gtaaaagcac
       61 gccggatgtc gaaacgccca gcctgaaacc gctgtggcgg gagctaagcc gggtggtgca
      121 gcataatctg caaaccgttc tcggttatga cttgttagcc ggccagcggg tattgcgcga
      181 gcagattgcc cgcctgatgc tcgacagcgg ctcggtagtc accgccgatg acatcatcat
      241 taccagcggc tgccataact cgatgtcgct ggcgttaatg tcggtgtgta aaccgggcga
      301 tattgtcgcg gtcgaatccc cctgttatta cggttcgatg cagatgctgc gcggcatggg
      361 cgtgaaagtg attgaaatcc caaccgatcc agaaacaggc atcagcgttg aagcgctgga
      421 actggcgctg gaacagtggc caattaaagt catcattctg gagccaaact gtaataatcc
      481 gctgggattt attatgccgg acgcgcgcaa acgggccgtt ctctctctcg ctcagcgtca
      541 tgatattgtg atttttgaag atgatgtcta cggcgaactg gcaacggagt atccgcgccc
      601 gcggaccatt cattcctggg atatcgacgg gcgagtgctg ttgtgcagct cgttcagtaa
      661 aagtattgca cctggcctgc gcgtgggctg ggtcgcaccg gggcgctatc acgataaact
      721 gctgcatatg aaatatgcca tcagcagctt taatgtaccg tccacgcaaa tggcggcggc
      781 aacgtttgtg ctggaaggtc attatcaccg ccatatccgg cggatgcggc agaattatca
      841 gcgcaatctg gcgctttata cctgctggat acgggaatat tttccctgcg aaatctgtat
      901 tacgcgcccg aaaggcggat ttttactgtg gatagaattg cctgaacagg tcgatatggt
      961 ctgcgtcgcg cggcagctat accgcatgaa aatccaggtg gcggcaggct cgattttctc
     1021 ggcttccggc aaataccgta attgtctgcg catcaactgc gctttgccgc tcagcgaaac
     1081 ctatcgcgaa gcactgaagc aaattggcga agccgtgtat cgggcaatgg aatgaattcg
     1141 aaatagtggc gagccccctg atttcctctc acgaatagcg tgtgactcgt tttatctttg
     1201 cagggtcatc tccacgttcc agcatggctt aggcaaaatg cgcttcgttc atgccggatg
     1261 ccatgtgaac gccttaccag gtctacaaaa tttttaaagt tcaatatatt gaatgggtta
     1321 tgtagacctg ataagcgtag cgcatcaggc agctttgcgt ttgtcatcgg ccactgatta
     1381 gtactcgatc catggatcgc gcttgctttc aggcaagggc tgatctaatg cctgcatttg
     1441 ctctgccgaa agtccggtaa tcttttgtat ccattcagga tccgccccat tctgcaacaa
     1501 caatcggagg aggctctgtt ccccttcttc tttcccttct ttcctccccc tctcccttcc
     1561 caacaaccat ccatcattat gaattcgctc tgcaatcgtc attattcgct ccctgtgttg
     1621 tggcattcga cgggttagtt cactgataaa ctctttaaaa ctcgcttcat cgccagccag
     1681 taaaatgtaa tttaacagcg cggttatctg gctgtcatta gcgcaccctg taaccagtaa
     1741 tactgccagt tgatcaataa gccccattag atcgcgctgg cgaatatgct tttggatcaa
     1801 ctccagcagg gcgactctgc gatgctgcac aatctcgtcg tctggcacga cagtgatatc
     1861 caccagcggg aacgcggcgg tataaagctt ccgtgcggta gtcgggtcgg caaattcgtc
     1921 cagccagcac aaagaccagg gataagggct acggctaccg tgataaaaca gcatcgggat
     1981 gaccagcggt agctgccggc gtttatcatg ttcgatatgg cgctgcatca ccgccatgga
     2041 atatcgcatc agacgaaagg ccatatgaat atcctcacgg ctctgatgtt caatcactac
     2101 ataaatatag ccatcgcctt cacgggtctt taccgaccac agaatatcgg aatgtagcgc
     2161 ccgcaatttt tcatcgacaa agctggcgga ttccagtttt aagctatcga gatcgcacag
     2221 ttcacgtaaa tctttgggta agtggatctc cataaaatcc cgcgcggtgt cagagtgtgt
     2281 gagaaaggat ttaaataatg cgtcatgcgg cgtgctggtc gtaaagtttg tcatggcgat
     2341 ccgtcacctg tagataagat ggcccaactt tagcgactaa caaagccatt cacacctggc
     2401 tatttcctat tccccgaggc ggtttccagg ctatttttat tcttctgcaa acgatacatt
     2461 tttatggaag atatctcgct ttttgcgttt tcttaccgtc aggctctatt cttacgttat
     2521 gaataaactc attgaactca gacgcgccaa aatgttggcg ctctctttac tgcttatcgc
     2581 cgctgctacc tttgtcgtta cgctgttttt gccgcccaat ttttgggtga gcggcgtgaa
     2641 ggcgattgct gaagcggcga tggtcggcgc actggcggac tggtttgcgg tggtggcgct
     2701 gtttcgccgc gtgccgattc cgatcatttc tcgtcatacg gcgattatcc cgcgtaataa
     2761 agaccggatt ggcgaaaatc tcggccagtt cgtgcaggaa aaatttctcg atacccaatc
     2821 gctggtggca ttgattcgac gtcacgaacc ggcgttgttg attggcaact ggtttagtca
     2881 gccggagaac gcccgccgcg ttggtcagca tctgttacag atcatgagcg gttttctcga
     2941 actgaccgat gatgcgcgta ttcagcgcct gcttaagcgc gcggtccacc gggcgatcga
     3001 taaggtcgat ctttccggca ccagcgcact gattctggag agcatgacca gaaacgatcg
     3061 tcatcaggtg ctgctcgata cgctgatcgc acagttgatc gcccttctcc agcgcgataa
     3121 atcgcgcaag tttatcgccc agcaaattgt tcgctggctg gagagcgagc atccactgaa
     3181 agccaaaatt ttgcccactg aatggctggg cgaacatagc gcggagttgg tttctgacgc
     3241 ggtgaat
//
LOCUS       DAWDRC010000467         3246 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11672_length_3246_cov_2.305233, whole genome shotgun sequence.
ACCESSION   DAWDRC010000467 DAWDRC010000000
VERSION     DAWDRC010000467.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11672_length_3246_cov_2.305233"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          736 a          876 c          810 g          824 t
ORIGIN      
        1 gagcaggagt gggaaaaact ggacgcagcc gatgccatta tcttcggtgc tcccacctat
       61 atgggcggtg cgccatggca attaaagaaa tttgctgatg ccagttccaa agcgtggttt
      121 acacgtaaat ggcaggataa agtcttcggg ggtttcacca acagcgccag cctgaatggc
      181 gataaacaag ttacgctgat ctatctgcaa acgctggctt cacagcatgg cggtctgtgg
      241 gtgagtttag gtcaggctcc tgccaatgtg cttgcgtcaa ccagggaaga tgtaaacaat
      301 ctgggcggtt cagtcggttt gctggtgcaa tctccagccg atgcgggtgc agaccaaatc
      361 cctacgggcg atctggatac agccgtgaaa tatggtgaac gcgtggcgac aattactgcg
      421 cgcttaaaat gatgattgcc gaatgtgacg ctgacgcgtc tatccggtct tctacatcta
      481 aaaagtgccg tcccctggtc agtcagataa agttttaacg ctgtatccga taacagttca
      541 acatgtacca tgtaccaaaa gaaaaaaggc cagcctcgct tgagactggc ctttctgaca
      601 gatgcttact tactcgcgga acagcgcttc gatattcagc ccctgcgttt gcaggatttc
      661 gcgcaaacgg cgcaggcctt caacctgaat ctggcgaaca cgttcacggg tgaggccaat
      721 ttcacgacct acatcttcca gtgttgccgc ttcgtacccc agcaaaccga atcgacgtgc
      781 cagcacttca cgctgtttgg cgttcagctc gaacagccat ttgacgatgc tctgcttcat
      841 atcgtcatct tgcgtggtat cttccggacc attctctttt tcatcggcca ggatgtccag
      901 caacgctttt tcggaatcac cacccagcgg ggtgtctacc gaggtaatgc gctcgttaag
      961 acgaagcata cggctgacgt catcaactgg cttatccagt tgctctgcga tctcttccgc
     1021 acttggttcg tggtccagct tatgggacaa ctcacgtgcg gttcgcaggt aaacgttcag
     1081 ctcctttacg atgtgaatcg gcaaacgaat agtacgggtt tggttcataa tcgcccgttc
     1141 gatcgtctgg cgaatccacc aggttgcgta tgttgagaag cggaaaccac gttccggatc
     1201 aaacttctct accgcgcgga tcagccccag gttgccctct tcgatcaggt ccagcaacgc
     1261 caggccacga ttgccataac ggcgggcaat ttttaccacc agacgcaagt tactctcgat
     1321 catccggcgg cgagaggcga catctccacg cagtgcgcga cgcgcaaaat aaacttcttc
     1381 ttcggccgtt aacagtggtg aataaccaat ctcaccaagg taaagctgag tcgcgtccag
     1441 cacacgctgt gtggctccct gcgataacag ttcctcttcg gccaaatcgt tatcactggg
     1501 ttcctcttct actaaggcct tttcgtcaaa aacctcaact ccgttctcat caaattccgc
     1561 atcttcattt aaatcatgaa ctttcagcgt attctgactc ataaggtggc tcctacccgt
     1621 gatcccttga cggaacattc aagcaaaagc ctggttccgc cgatttatcg ctgcggcaaa
     1681 taacgcagcg ggtttacgga tttccccttg taacgaattt caaaatgcaa gcgtgttgaa
     1741 ctggttccgg tgctacccat ggttgctatt ttttgccccg ccttcacttc ttgttgttcc
     1801 cggaccagca ttgtgtcgtt atgggcgtag gcactcaggt aatcatcatt atgtttgatg
     1861 ataatcagat taccgtagcc gcgcagcgcg ttaccggcat aaacaacgcg gccatccgcg
     1921 gtcgcgataa ttgcctgtcc tttgctgcct gcgatatcaa tccccttgtt gcccccctca
     1981 gaagcgccaa aggtttcgat cactttgccc tcagtcggcc agcgccaggt ggagataggc
     2041 gtactggttg atgtactgct gacagtcggc tcggttgtgc ttgctgttgg taccgttaca
     2101 ggcactgtga ccgtggtcgc agttggcttg ttgttcggca acattttgtt agcactctgt
     2161 tcacccgaag actcagaata cgtaattgtc ggttgcgacg caacagcaac ggtggaattt
     2221 tgtgcaggct tgatcacaac tccttgctct gctgcgtcgg cctgggtaat ggcatttccg
     2281 cctgtgattg gcgtaccgga agcattaccc acctgtaagg tctgaccgac gttcagcgcg
     2341 tatggtgcct gaatattgtt gcgctgagca aggtcacgga aatcgttgcc agtaatccag
     2401 gcgatataaa aaagcgtgtc gccttttttc acggtgtatg tactgccgct ataactgcct
     2461 ttcggaatgt tcccatactg acggttgtag acgattcgtc cgttttccat ctgtaccggc
     2521 tgctgagcta ctggctgcac tggctggatt tgcggttgtt gagtagcctg aatttgtggc
     2581 tgctgtaccg gctgaatttg cggttgctgc gctgtagacg tcgtccccat tttcggcggc
     2641 ggcgtaatca acataccaga attggtattt gcaggcgcat tgccattaac ggagctgacc
     2701 ggtgccggtg gatttgaagt gtcagaacag cctgccagcc atagcgaaac cagtgacaat
     2761 gccgcaatgc ggcgaacggt gaattttggg cttcccgcgc tcatttatcc cccaggaaaa
     2821 attggttaat aaccagtgac ataattaccg tgcaaggcac catactgaac actggaaaag
     2881 atgttcacga tacgctgact tgcggcaaaa taaccaggaa aaatccaggt atttcctcac
     2941 gttttaagcc agctcaccct tcactaaagg gacaaagcgc acggcctcca cggtatcgat
     3001 aataaattcg cctccccgac gacgcacccg tttcaaatac tggtgctcct cccctacggg
     3061 taagacgaga atcccgcctt cgtccagctg cgtcattagc gcagttggaa tttcaggcgg
     3121 tgccgccgta acaatgatag cgtcaaacgg cgcgcgtgcc tgccaacctt gccatccatc
     3181 gccatgacgg gttgaaacat tatgtaaatc aagatttttc aggcggcgac gcgcctgcca
     3241 ctgcaa
//
LOCUS       DAWDRC010000468         3243 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11686_length_3243_cov_1.926600, whole genome shotgun sequence.
ACCESSION   DAWDRC010000468 DAWDRC010000000
VERSION     DAWDRC010000468.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11686_length_3243_cov_1.926600"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1654..1663
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          792 a          915 c          855 g          671 t
ORIGIN      
        1 ggtcggcatc cattacgaca gtgccgccag ggagaaatac ccccaacgat cccggtgaac
       61 tcatggcgta gttatgagcc gccgggatcg cgcacaggta gcgcgtctgt tgtgtgaact
      121 gacaaatctc gacgctacga cgcacgctgt ggtagtagtc gttatgagtg cgcgggatca
      181 gtttcggagt gccggtggtg ccgccggaaa gctggaaata ggccacttca tcagcaggag
      241 atggcgtggc agtaaaatcc tcagccggat ggttaatcgc atcctgcaag ttatgctcac
      301 cgctgtcgtt gagcagctgc accacgcgaa tcgaagaata ctctgcaaca aatgtgttga
      361 ggaaatcatc cccgctaaat agcgcatgtt ggcgatcggc aatcagtaac gccggttcaa
      421 tctggctggc ataggcgttc agttcactac gctgatggct gaacagcgcc agcaccggcg
      481 caacgcccag tttcagcagc gcgaaaaagg taatgtaaag ttcagcgacg ttacccagtt
      541 gtaccagcgc ggtttcacca ggtttaatgc cctgacggcg caaactacag gcgaggttat
      601 ccgccgcctg attcagctcc cggtaactca actgccgctc gccgtcgata accgcgatgc
      661 tgtcactcgt agcgtggcga gtcagaatgt cggtcagtgg caaatcctgc cagtagcctt
      721 tttcccgata gcgacgggca aactcttccg gccagcgggt aaatggaatg ctcatcctcg
      781 ctccttaatg caatccaaaa acgttcaaca tggtagaaag tttgacgccc gtttcgcgcc
      841 actcacccaa cggtgaggac gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat
      901 tttcccgcag cttcgcgcag cggatggtca ccacccattc accgttacct tcgctgtcac
      961 accaacccac aatgccgcca aacagttcgc ggtcgaacgg ctccagttca gcaataacct
     1021 gagtcgcggc ctgatgcagc agacaagcca aagtcagtgc gttttcttgc gaattcgctt
     1081 taccttcaaa gggcgttgcg agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg
     1141 aaggaacgtg taactcacta ctgcgtttgc gcagtacctc tttcatcgcc tgagtcacca
     1201 gttcatgttc atggcgatct ttttctgacg ccagcagacg attacccgct tcgcgatcga
     1261 gcacttcatc cggctgacga cgcgcggaac cggctaacgg aatggagcta aaacgctcgc
     1321 cgtctttacg tagcagcagt tccgggctgg cccccagcag aacgccacca tcagccaacg
     1381 gaacatggaa gttgtaacta accgggtttt gcgcaatcaa ccgttccagc aatacgccac
     1441 tatcaatggc ggcgtcagta gtgatatcaa tcaaccgtga caacaccact ttgtcgacct
     1501 gcggcgtggc ggtaagtgcg gcagcacggg caaccatctg ttcaaacgtg gtttgttccg
     1561 gaatggcctg acgttccacc acgttcagcg actggctgcg agtgaaacgg cgtgcggaag
     1621 cttgtttttc ctgacgggag aacgactgcc aggnnnnnnn nnngcgacga aggctgacgt
     1681 ggatcgaagg gaatcgcccc gaccatcacc ggatttttga tgccctgcgc tttggcatcg
     1741 gcaaacaacg cggcgagttt ttgctggaag ggactgtcgg gcgaatcccc gttcacagcc
     1801 ggttcatcga agcgggcgaa acatcctgac gtcgtaaaac tgcggtacgg cgacataaag
     1861 aaaaagcgat tgggcgcaag tgttgccatg gtctgctgta cttcctcagc cagtgacgta
     1921 tccatatcat cctccacaaa atgataaagg ctttaataat gattatcatt tatattttcg
     1981 gtcgctaacc taaaagcaaa cgcgcactat gtcaactctt gaggtaacgc aatctgcaac
     2041 tcggggttgc atctggttgt ggttacaatg aaaatgagaa gcattaactt cattaataac
     2101 aggaagctga cttgtgagac tcgccccgct ctaccgcaac gcccttctat taacaggact
     2161 tttgctttca ggaatagccg cagttcaggc cgccgactgg ccgcgtcaga ttactgacag
     2221 ccgtggcact catacccagg aaagccagcc gcagcgtatt gtttccacca gcgtcaccct
     2281 gaccggctca ctgctggcga ttgatgctcc ggtgatcgcc agcggcgcga ccacgccaaa
     2341 taaccgcgta gcggatgacc agggcttttt acgccagtgg agcaaggtgg cgaaagaacg
     2401 caaactgcaa cggctctata tcggcgaacc gagtgcagaa gccgttgccg cgcaaatgcc
     2461 ggatctgatt ttaattagcg caaccggcgg ggattcggcg ctggcgctgt acgatcagct
     2521 ttctaccatc gccccaacat taatcatcaa ttacgacgac aaaagctggc agtcgctgtt
     2581 aacgcagctt ggcgaaatta ccgggcatga gaaacaagcg gcagagcgga ttgcacagtt
     2641 tgataagcaa ctggcggcgg cgaaagagca aatcaaatta ccgccgcagc cggtcactgc
     2701 ccttgtctat accgccgctg cacacagtgc caatctctgg acgccggaat cagcacaagg
     2761 gcagatgctg gaacaactcg gctttacgct ggcgaagttg cccgcagggt taaacgccag
     2821 ccaaagccag ggcaaacgcc atgacatcat tcagcttggt ggagaaaatc tggctgcagg
     2881 gttaaatggc gagtcactat tcctgttcgc cggtgatcag aaagacgccg atgctattta
     2941 tgctaatccg ctgctcgcgc acctgcctgc agtacaaaac aagcaggttt atgcgctggg
     3001 aaccgagacg ttccgcctgg attactacag cgccatgcaa gtgctggata gacttaaggc
     3061 gctgttttaa gcattaactg tcggacgctg tcacctgcgg cggcgtctgg cgaaaacgtc
     3121 gcaactccac tagtaccagc agcaacaaca cgccgataat caacaaacca aaaccgctcg
     3181 cgcttgcgga agcaaccggt gtcatcatcg ctcccagacc gcctaacagc gccgcaccta
     3241 tgg
//
LOCUS       DAWDRC010000469         3236 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11709_length_3236_cov_2.255580, whole genome shotgun sequence.
ACCESSION   DAWDRC010000469 DAWDRC010000000
VERSION     DAWDRC010000469.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11709_length_3236_cov_2.255580"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    320..329
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2616..2625
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          797 a          857 c          748 g          814 t
ORIGIN      
        1 atattaagtc gtcgatagaa acaagcattg aaaggcacag cagtagtcaa acagtgtgaa
       61 acgctactgg cgccttacag cgcaaaaagg ctggtgacta aaaagtcacc agccatcagc
      121 ctgatttctc aggctgcaac cggaagggtt ggcttattta acttcaactt cagcgccagc
      181 ttcttccaga gcttttttca gtgcttctgc gtcgtctttg ctcacgcctt ctttcagagc
      241 agccggtgca gattcaacca ggtctttagc ttctttcaga cccaggccag ttgcgccacg
      301 tactgctttg ataacagcan nnnnnnnnnc agcttcaacc gggccagcag ctacagctac
      361 agcagcagca gcggaaacac cgaatttttc ttccattgca gagatcagtt ctacaacgtc
      421 cattacagac atagctgcaa ctgcttcaat gatttgatct ttagtgatag acatttaaat
      481 tgttcctgaa tatcagaata agtttatacg taagcgaatg cgttaaaaag ataactgcga
      541 ttaagcagct tctttcgcat cgcgtacagc agccagagta cgaaccagtt tgccagccga
      601 agcttctttc atggttgcca tcaggcgtgc aattgcttct tcgtaggtcg gcagagttgc
      661 caggcggtcg atctgagacg ccgggatcag ctcaccttca aaggcagcgg ctttgacctc
      721 aaattttgca ttcgctttcg cgaactcttt gaacagacga gcagcagcgc ccgggtgttc
      781 catagagtat gcaatcaggg tcggaccaac aaacgcgtct ttcaggcact cgaacggagt
      841 accttcaaca gcacggcgca gcagggtgtt acgaacaaca cgcatgtata cgccagcttc
      901 gcgacctgct ttacgcagtt cagtcatttt atctacagtt acgccacggg aatccgcaac
      961 tactgcagac agcgcgcctt tggctacttc gctgacttca gcaacaatcg cttgtttgtc
     1021 ttgaagattt aaagccatta gctttgctcc tggatgtttg ccagagaaaa tctctggaac
     1081 tcacttcact cctccaaacg gagagagcgt cttaattacg gtgagcagaa acaagccaga
     1141 atataaaaga ataatcttag cgttctgtca ccgtctacgc aggggattaa gtttcttgcg
     1201 aaactcctgc ggtcttcgac ggaggcctgg ataggccagg ctccaacgaa caaatctttt
     1261 tagctgctaa ctttcgttag catacgtggg ggtaagattg tagacaaaat caccgcccac
     1321 gtaaaggcat tagtttacag aagcgctcag gccagcctgg tcaactgcaa cacctgcacc
     1381 catggtggta gagatgctaa ctttcttgat gtacacgcct ttcgcctgag tcggttttgc
     1441 ttttttcagc gcaaccagca gagcttccag gttttctttc agtttgtcag cgtcaaagtc
     1501 cactttaccg atggtggtgt ggatgatgcc gtttttgtcg ttacggtaac gaacctggcc
     1561 agctttagcg tttttaaccg cttcagcaac gttcggcgtt acagtaccca ctttcgggtt
     1621 tggcatcagg ccgcgcggac ccagaacctg gcccagctgg ccaacaacgc gcattgcatc
     1681 cggagaagca ataacaacgt caaagttcat ttcgcctttc ttgatctggt cagccagatc
     1741 ttccatacct accagttcag cacctgcagc tttagcagct tcagcgtttg caccctgggt
     1801 aaatacggct acgcgaacgg aacggccagt accgtgcggc agtacagttg caccacgtac
     1861 gttctggtca gatttacgag cgtcgatgcc gaggttaaca gctacgtcca cgctttctac
     1921 gaatttagca gtcgccagtt ctttcagcag agcgatagct tcgttgatgt cgtactgttt
     1981 ggttgcatca actttctcgc ggataacacg catgcgcttg gtcagtttag ccatttctta
     2041 gtcctccact accaggccca tggaacgtgc agtaccttcg atggagcgag tcatcgcttc
     2101 aatgtcggca ccagtcatgt cggcagcttt ggtctgcgcg atttcctgca gctgagcgcg
     2161 ggaaatttta cccactttgt ctttgttcgg cttaccggaa ccagacttga taccagccgc
     2221 ttttttcagc agaactgctg ccggcggagt cttggtaacg aaagtgaaag aacggtcagc
     2281 gtaaacagtg attactaccg gaatcggcag acctttttcg atggaatcag tttttgcgtt
     2341 gaacgctttg cagaattcca tgatgtttac gccctgctga cccagagccg gacctactgg
     2401 cggactcggg ttagccatac cagctgcaac ctgcagcttg acataggctt gtactttctt
     2461 agccattata aattcctcaa gttgggtaat aacgcctctg agaggctccc cgtgatataa
     2521 aaaatcgttt tacgggcaag gcccataaaa acaaaaggcg cgaaattgta ttccaatctc
     2581 gcgccttgtg caacgattaa atcgccgctt ttttgnnnnn nnnnnacctg gctgaagtcc
     2641 agctctacag gggtcgcacg accgaagata gaaacagaca ctttcagacg agatttctcg
     2701 taatcaactt cttcaacaac gccgttgaag tcagcgaacg gaccatcatt aacacggacc
     2761 atttcacccg gttcaaacaa cgttttcgga cgcggcttat caccaacctg ctgcaggcgg
     2821 ttcataatcg catcgacttc tttatcgctg attggcgcag gacgatcgga agtaccgccg
     2881 atgaagccca tcacacgcgg tacgctgcgc accaggtgcc agctcgcgtc gttcatcacc
     2941 atctgaacga ggacgtagcc agggaagaat ttacgttcgc ttttgcgacg ctgaccgcca
     3001 cggatttcaa ccacttcttc ggttggtacc atgacttcac caaacaactc ttccatgttg
     3061 tgtaatttga tatgctcacg cagcgacgtt gctacgcggc cttcaaaacc ggaaaacgcc
     3121 tgaacgacgt accagcgctt tttaggagct tcagacatct cagaacctca ggccagtgat
     3181 aaaggatacc aggcgaacca gaataccatc cagtccccac aggatcagtg acattc
//
LOCUS       DAWDRC010000470         3233 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11715_length_3233_cov_2.574575, whole genome shotgun sequence.
ACCESSION   DAWDRC010000470 DAWDRC010000000
VERSION     DAWDRC010000470.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11715_length_3233_cov_2.574575"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          777 a          846 c          806 g          804 t
ORIGIN      
        1 gggtaacgat ttctttaatc agcggcggac ctttaaaaat aaaaccagaa taaatttgca
       61 ccagtgaggc acccgcagca atcttttcac gcgcagcgat aaccgagtca atgccgccaa
      121 caccaataat cggcaagcgt ccgtttaatt cctgtgacaa gcggcgaata atttcggtgc
      181 tttttaactg aagcggacga ccacttaagc caccggtttg atcgcaattt ttcattccct
      241 gaaccagaga acgatcgagt gtggtattgg ttgcaataac gccatcaata ttatggcgaa
      301 ctaaactatc ggcaacctgg atcaattctt cttcagaaag atccggcgcg atcttcactg
      361 cgatcggcac atatttatgg tgcatcactt gcaaatcatt ttgtttattt ttaatcgcag
      421 ttaagagatc atccagcgct tcaccatatt gcagtgtgcg taatcctggg gtatttggcg
      481 atgaaatatt gatggcgata tatcccgcat aggcatagat tttttccata caaatcagat
      541 agtcatcttt accctgctcc accggcgtat ctttattttt gccgatgtta atacccagaa
      601 cgccgtcata atgggctttt tttacgttct ctacgaggtt atcaacgcca agattattaa
      661 agcccatacg gttgatcaaa ccttcggcat ctaccagacg aaagagacgc ggcttgtcat
      721 tacctggctg gggacgtggc gtgacggtac cgatctcgat cgatccaaat cccattgcgc
      781 ctaacgcgtc aatgcactcc ccgtctttat caagaccggc tgccagacca agcggatttt
      841 taaacgtcag gcccatgcag ttaacaggtt tcgcaggcac tttctgccgc accagtgctt
      901 caaacggcgt tcctgtaata cggcgtaatt gctgaaaagt aaactcatga gcgcgctctg
      961 gatcgagctg gaaaagggct ttacgaacga aggggtagta catgaactct cctggattcc
     1021 cggtgtgcaa accgggggcg tattatgtgc gagttcggac caaaagggaa ttgacctgcg
     1081 gcaataaata gcaaacgttt tcttattgcg ctttttttat gctttttttg gcttttcttc
     1141 ctcagataaa tcatttcgac tcgcggacgc cctctgtcag actgaccgaa ttgttatcaa
     1201 tgttaacaaa acaagaacaa ttggttataa ggagagagta tgcgtgtcat caccctggcg
     1261 ggtagtcctc gctttccttc tcgctccagc tccttgctgg aatatgtgcg ggaaaaacta
     1321 aatggcctgg atgtagaggt ttatcactgg aatttgcaaa acttcgtccc ggaagatctg
     1381 ctttatgctc gtttcgatag tccggcactc aagaccttca ccgaacagct gcaacaggcc
     1441 gatgggctga ttgtcgccac gcctgtgtat aaagccgcct attccggtgc actgaaaacc
     1501 ctgctcgacc tgctgccaga acgtgcgttg caaggcaaag tggtgctgcc gctggcgaca
     1561 ggcggcaccg tggcccatct gctggcggtc gattatgccc ttaagccagt tttaagtacg
     1621 ctgaaagccc aggagatcct gcacggcgtg tttgccgatg actcgcaagt cattgattac
     1681 caacacaaac ccaagttcac gccaaatctg caaactcgtc ttgataccgc gctagaaact
     1741 ttctggcagg cgttgcaccg ccgcgatgtt caggttcctg accttctgtc tctgcgaggt
     1801 aatgcccatg cgtaagatca ttaaactggc gctggcgggt ttacttagcg tctctacact
     1861 tgctgttgcc gcggagtctt cgccagaggc gttacgtata ggctatcaaa aaggcagtat
     1921 tggtatggtg ctggcaaaaa gccaccagtt actggaaaaa cgctatccgc aaacaaaaat
     1981 ctcctgggtg gagttccccg ctggcccaca gatgctggag gcgttaaacg ttggcagtat
     2041 cgatctcggc agtaccggag atattccgcc aatctttgcc caggctgccg gggctgattt
     2101 ggtatacgtg ggcgtcgagc caccgaagcc caaagccgaa gtgattctgg tggcagaaaa
     2161 cagcccgatc aaaaccgtag ccgatcttaa aggtcacaaa gttgcctttc agaaaggttc
     2221 cagttcgcac aatcttttac tgcgagccct tcgccaggcc gggcttaaat tcacggatat
     2281 tcagcccact tacctgacgc ctgccgatgc ccgcgccgcg ttccagcaag gtaacgtcga
     2341 cgcctgggct atctgggatc cctactactc cgctgcatta ttacagggcg gcgtgcgggt
     2401 gctgaaagac ggcaccgatc tcaatcaaac tggatcgttt tatctggcag ctcgtccgta
     2461 tgcagaaaaa aacggcgctt ttattcaggg tgtgctgaca acctttagtg aggccgatgc
     2521 gttaacccgc agccagcgcg aacaaagcat cgctttactg gcaaaaacga tgggcttacc
     2581 ggccccggtg attgcctcgt atctggatca tcgtcctccc accaccatca aaccgttgag
     2641 cgctgaagtt gccgccttac agcagcaaac ggcagatctg ttttatgaaa accgtctggt
     2701 gccgaaaaaa gtcgatattc gccagcgcat ctggcaaccc actcaactgg aaggaaaaca
     2761 attatgagtc tgaatatgtt ctggttttta ccgacccacg gtgacgggca ttatctggga
     2821 acggaagaag gttcacgtcc ggttgatcac ggttatctgc aacaaattgc gcaagcggcg
     2881 gatcgtctgg gctataccgg tgtgctgatc ccgacggggc gatcgtgtga agatgcgtgg
     2941 ctggtggcgg cgtcgatgat ccctgtgaca caacggctga agtttcttgt cgccctgcgc
     3001 cccagcgtaa cctcacctac cgttgccgcc cggcaggccg ccacgcttga ccgtctctct
     3061 aacggacgtg cgttgtttaa cctggtcaca ggcagcgatc cccaagaact ggcaggcgac
     3121 ggcgtgttcc ttgatcatag cgagcgttac gaagcctcgg cagaatttac ccaggtctgg
     3181 cggcggttgt tgcttggcga gactgtggat ttcaacggca aacatattca tgt
//
LOCUS       DAWDRC010000471         3232 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11721_length_3232_cov_2.559333, whole genome shotgun sequence.
ACCESSION   DAWDRC010000471 DAWDRC010000000
VERSION     DAWDRC010000471.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11721_length_3232_cov_2.559333"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          936 a          661 c          776 g          859 t
ORIGIN      
        1 tcaatccaat aaatgctaat attatggcta tcatagccat aaaaagataa aatattatca
       61 ctatgggtaa aaaaacagca ccgttactcc cttatacgaa tcagatgctt agtgaatttg
      121 gtgagcggct gaaacttgct cgtttacgac gcaggcttac cgcaaagcaa gttgctgaac
      181 gtgctgggat gtcgccaata acattgcgct ctctggagtc aggaagtgca ggggtcacga
      241 taggggcata tttgtctgtg atgcaggttt tgggcctgga aacggattta aataagcttg
      301 cagctgaaga cgacttaggg cgtcagctcc aggattcccg tttgattaaa aaaagagggc
      361 tttctcgttc atcaggtaaa aatgaagaag gtattcagac agaccctttt caaacgaggt
      421 atgagaaaga aggtttactc accacggatt taacagcact ggcagaaggc tcaacgccat
      481 ctattacagg agaaacatca acctctcttg cagcactatt aaaaccgata aaaaaaccgt
      541 taaaaggaaa ttaattatgg cattaatcgg agtgtatgcg gattgggaag gcctggatgg
      601 tccggagcgg attggttatt tacacagtcg ccgcactcgg acgcgtgaga tatttgagtt
      661 tgagtacgat aaaaaggcgc ttgcagatcc ttcacttaat ttcattcagt tagatcctga
      721 aataatgttg tatgagggag cacagtaccc aattcctcca aaggataaat ttggtgcatt
      781 tagtgattcc tgcccggatc gttgggggcg tatgctaatg aagcgtcgtt ttgagaggga
      841 tattcgagac gggttatgtg ataaagactc ccacctttat gagtctgatt atctactagg
      901 tgttcatgat ctttatcggg ttggggctct tcgatacaaa cgtgaagacg ctggagagtt
      961 tcttgataat cgcattgatg ttgcagccca ccatttaccg aaatagcgag tctggaaagg
     1021 gctagccggg caattgaaga agatcctgat aataaagagc taatggggca aaaatggtta
     1081 agaatgctaa tcgctccggg gggatcatta gggggggcac gtccgaaagc cagtgttgtt
     1141 gatgaagctg ggcatctcta catcgctaaa tttcccagtg taaaagatga gtatgatgtt
     1201 ggtggatggg aaatggtcgt taatgcactt gctgttggct gtggactgaa tgttgcacct
     1261 gctcaggcgc ataaatttgc cagcaattac cattgtttta tggttcgtcg ttttgaccgc
     1321 actaatgcgg gaaggcgtct gcattttgct tcggctatga cactaaccag gcatcaggat
     1381 ggtgaagatg cctctacagg tgtgagttat ctggaattag cagatgttct tatcaggcat
     1441 ggcgcgcaga ctaatactga tcttaaggaa ctctggtcca ggattgtttt taatattctt
     1501 gtttctaata ccgatgacca tttacgtaat catggatata tcttgatacc gggaaaaggt
     1561 tggcggcttt ctgaggcata tgatttgaat cctgttgcca ggtctgacgg tctaaaactc
     1621 aatattacag aaaatgataa tgcacttgat ttggaattag ctcgagaagt tgcggagtat
     1681 tttcgacttg gtttgacaga agcagatgac attattgcta attttagagg gattgtcagt
     1741 cagtggcgaa tcattgccga gcgtcttcga ttacctggtc gtgagcaaga gttaatggca
     1801 gaggcgttca ggggggcaat ttagaatagt tatttcgata tattcgctaa atcataaatt
     1861 tgcgaagcaa tgccagtaga tactgaacgt tttggaagag acgtttaatt tatgagaaaa
     1921 atagaaacgc tacaaagcta aattatagcg tttctatgta aaataattag cttgttgcta
     1981 actgtacttg tggttcttcg tctttaactt tgataagcgt ccacagcgca atggcgccaa
     2041 taatgtcaaa gaaagccagg gcaataaaga acggcgcgaa gccaataatg tgaacgaatg
     2101 ccccgataaa caggttgaaa atcaactgac cgctccatgc acaggctcct gccataccca
     2161 cagcggtagc tacttcgtct tttttgaata ggtcaccgcc cagagttgct gcaacagtag
     2221 aaagacattg atgcgagaat gcgccaatgc taatcagtaa tacggcgatg tagggatttt
     2281 cgacaatact caccagacca atcgtcatca taatgacggc tgcaatagtg aaggtaattc
     2341 ttcgtgagtt aattaaactc acgccgcgat cgtggaagaa cttcgcgaca aaaccactgg
     2401 ctaaacagcc gagatcgccc agcaggaaag gcagccagac gaacatggca atttctttca
     2461 ggctaaaatg cagcgtttcg acgaagaaaa tcggtaccca gaagttaatg gttccccatg
     2521 ccgggtcagc gagaaaacgc gcgatgccaa tcccccagaa attacgttgg ctgaggatat
     2581 gcttgattga cgttttttct ttcttatctg ttttaagatg ctgttcttgc ccagactcaa
     2641 tatagtggcg ctcttcatca gaaaggcgtt tagcatcttt tggatcttta taacaaaaga
     2701 accaaaataa agccgcgagt aaagcaaggc tacctgagac aataaacgca aattgccagc
     2761 tatgaaacat aatgcaccag gcaatcaacg gtggagcaag catcgcgccg agtgaggtgc
     2821 ccatattgaa aacacctgtc gccacgccac gctcttttgc cgggaaccat gttgatgcgg
     2881 tttttacacc cgcaggaatt gccgatgctt cgctgaaacc catcagggcg cgcataaatg
     2941 ccagacctga ccatgttcct gtcaaggcat gccccattgt cgccaggccc cagattgcgg
     3001 cacatatcgc aaaacttaac ttcagaccca tggtatcaat aaagaagccc attatcggtt
     3061 gccccaatgt ataggccaac tggaatgcac tgacaatcca tgaatattgt tctttggtta
     3121 ttccggttgt ctccataatc gttggagccg caatacctaa cgccgttctg tccagataat
     3181 tgattacagt gataaagcat acgagggcaa tcatatgcca tcgataattc tt
//
LOCUS       DAWDRC010000472         3229 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11725_length_3229_cov_2.398551, whole genome shotgun sequence.
ACCESSION   DAWDRC010000472 DAWDRC010000000
VERSION     DAWDRC010000472.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11725_length_3229_cov_2.398551"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1788..1797
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          881 a          702 c          736 g          900 t
ORIGIN      
        1 aataaaaaac gaatgataca ttagataaat tttaaaactg ccaacaacat ctgaaatctt
       61 tgcgtttgat atttataatt attcaaactg aaacaacaca ggcatacgag ttgtttcact
      121 gtgaaacagt gcagcgaaat ttgttctgcc cttttatttc ctcaaagata tttcatcgtt
      181 aaattccaaa attgactgac tattcaactc agcgtaaata tcaggagaaa taatgtatgg
      241 cgaggatgcg tgcaatcgaa gctgcagtag aaatactgaa aaaagaaggc attagcgttg
      301 cttttggtgt acccggcgca gcaataaacc cactctatgc cgcgatgaaa aaatcaggcg
      361 gtattgatca tattctggcc cgtcatgttg agggcgcctc gcatatggct gaaggttaca
      421 cccgcagtca gaatggcaat attggcgtgt gtattggcac atcaggccct gcgggcactg
      481 atatgattac cgggctgtat tctgcctctg cggattccat tcctattctc tgcattactg
      541 gtcaagctcc cgtaggaaaa cttcataaag aagatttcca ggccgtcgat attgaggcta
      601 tcgccacgcc agtaaccaaa atggctcgca caattctcga agcgggtcag ttgccgggga
      661 tattccagaa agccttctgg gaaatgcgtt caggccgccc tggcccggtt cttttagatc
      721 tcccttttga tgttcagatg accgaaattg agtttgatat cgatctctat cagccactaa
      781 ccccctggca acctaaggca acacgggccc aggccgtacg cgcattagaa atgctaaatg
      841 acgccgaaaa gcccgtcatc atcgctggcg gtggcattat taacgccgaa gcgagtgaat
      901 tgttgcgtga gtttgtcgag ttaactggcg ttcccgtcat tcaaacctta cgcggttggg
      961 gcgctttatc agacgatcac cccctaatga ttggtcgcat gggatgtcag gcgggtcatc
     1021 gctatggtaa cgccagttat ctggcctcag attttgtttt tggtatcggt aaccgttggg
     1081 caaaccgcca taccggagcc attgagacct acaccgaagg ccgcaaattc attcatgttg
     1141 atatcgaacc tgcacaaatt ggccggatat ttgcgccgga tctgggcatt gtttctgatg
     1201 ctgaaagcgc cttaacgcta tttattcagg tcgcccgtga tatgaagtca cgtggagaac
     1261 tgaaagaccg cagtcgctgg attgccgaat gtaccgagcg taaacgcaca atgcttcgtc
     1321 gttcagactt tgactgcaat ccaattaaac cgcagcgcgt gtatcacgaa atgaataagg
     1381 ttttcggacc ggagacacgc tatatttcga caattggtct ggcgcaaatc gccgcaaacc
     1441 aatttttaca tgtttaccgc ccacgccact ggattaatgc ctgtcaggct gggccgctag
     1501 gctggacaat gcccgcagca ctgggcgcgg taaaagctga tccatctgtt cccgttgtgg
     1561 caatatccgg tgattacgat tttcaattct tgattgaaga attagccgtc ggcgcacaat
     1621 ttaatcttcc gtatatccat attttactta acaacgctta tcttggtttg attcgtcaat
     1681 cacaacgtgc atttgatatt gattattgcg ttcaactgtc atttgaaaat attaatgctc
     1741 cagaaattaa tggttatggc gttgatcata aagctgtggt tgaaggannn nnnnnnntaa
     1801 agcgattcgc gtattcgcat cacaggatat tgcgcctgca ctgcaagaag cccagcgttt
     1861 gcgtgacgaa tttcacgtac ctgttgttgt ggaaattatt actgaacgcg tgactaatat
     1921 cgctatggga cctgacatta ataaagtcac agaatttgaa gaaattctcg atttataagg
     1981 aaacaggatt atggcaaagt ttgcagcaaa tttatctatg ctctttaccg agtatccatt
     2041 tattgaacgt tttgcacaag caagtcacgc aggattccac ggcgttgagt atcttttccc
     2101 ctatgacttt tccgcagatg agttggtttc gcaactgcat caacataatc tgacgcaggt
     2161 tctgtttaat cttcctgcgg gtaactggca agagggtgaa cggggtattg catgtcatcc
     2221 tggtcgggca aaagaatttc aggagggcgt ttccagggca atagagtatg cactggccct
     2281 taattgttca caagttaatt gccttgcagg aaaacatcca ggtggttatt cgcatgaaca
     2341 atgtcatgaa acactggtcg aaaatttgcg ctatgcagta gataaattgg cttctcatgg
     2401 cataaagtta gttctggaag ccattaatac aaaagatatt cccggttttt ttgttaacaa
     2461 caccagacaa gctctcaata ttattcatga tgtaaatcat cctgattttc gctatcaata
     2521 cgatatttat catatgcaaa tcatggaagg gaatattgct acaacaatca aaaacaacct
     2581 gaataacatt ggacatattc aattagccga taaccctggg cgtcatgaac cagggacggg
     2641 agaaattaat tacccgtggt tgttaaatta tatagaccag attggttatc agggatggat
     2701 tggttgcgaa tatattccat ctacgacaac gactgaaagt ctacgctggc taaaaaacga
     2761 gactcaattt taatccctga ggtatttatc atgaatattg gatttatcgg caccggcata
     2821 atgggtaagc cgatggctta taatttacaa caagccggac atactcttta tttttcagcg
     2881 cattttgaac ctgcaccgca agagcttatt ggtgaacgtg gcattgtttg ctctacaccg
     2941 cgtgaagtcg cgcaggagtg tgaggtcatc atcaccatgc tccctgatac cccacatgtt
     3001 gaggatgttc tttttcatcc caactatggc gtaatacacg gtttaagcca cgggaaaatt
     3061 gtgattgata tgagttcaat ttccccggtt gccaccaaag catttgcaca acgtattatc
     3121 gcgggaggcg cggaatatat cgatgcgccc gtctctggcg gtgaagttgg cgcgaaagcc
     3181 gggacgcttt ccattatggt tggcggatgt gaagaggttt atttgcaga
//
LOCUS       DAWDRC010000473         3227 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11736_length_3227_cov_2.097100, whole genome shotgun sequence.
ACCESSION   DAWDRC010000473 DAWDRC010000000
VERSION     DAWDRC010000473.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11736_length_3227_cov_2.097100"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2166..2175
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2645..2654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          736 a          817 c          885 g          769 t
ORIGIN      
        1 ttagcgatgc ggatgccaat cacatgtttc tcgatccggt taaagtcact attttgacac
       61 cgggaatgga cgagcagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
      121 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
      181 tctttagtat tggcatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
      241 ttaagcgctc ttacgatctc aacctgcgga tcaaaaatat gctgcccgat ctctatgcag
      301 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
      361 tgattcgtaa acacgatctt cccggtttga tgttgcgggc atttgatacc ttgccggaga
      421 tgatcatgac gccacatcag gcatggcaga gacagattaa aggcgaagta gaaaccattg
      481 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccacccggcg
      541 taccgctgct gatgccggga gaaatgctta ccgaagagag ccgcacggta ctcgattttc
      601 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
      661 cgaaacagga cgaagacggt gtttaccgcg tacgagtcct aaaaatggca ggataacttg
      721 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggaggcg ttatgctggg
      781 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcgatga gcaaagcttt
      841 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
      901 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct catttccgtt
      961 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     1021 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     1081 ccgtgtcgat ccctacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     1141 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgcccgg
     1201 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctctcct
     1261 cacgccagat tctggtggcc tttagcggcg gccttgattc tactgtctta ctgcatcaac
     1321 tggtgcagtg gcggacggaa aatccgggcg tcactctgcg cgctatccat gtgcatcacg
     1381 gtttaagcgc caatgctgat gcctgggtga aacattgtga aaacatctgc caacagtggc
     1441 aggtgccgct ggtggtcgaa cgtgtgcagc ttgcgcaaga ggggctaggt attgaggccc
     1501 aggcgcggca ggcacgttat caggcatttg cccgcacctt gttgcccggt gaagtgctgg
     1561 ttacagcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     1621 gcggccctgc cgggctttcg gctatggcgg aagtttcgga gtttgccgga acaccgctta
     1681 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcgttg gcgcatggtt
     1741 tacgctggat tgaagacgaa agtaatcagg atgatagcta cgatcgtaac tttctgcgcc
     1801 tgcgcgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acagcccgca
     1861 gcgccgcact ttgtgctgaa caagagagcc tgttagatga actgctggcg gatgatttag
     1921 cgcactgtca aactccgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     1981 cccgtcgcgc ggcgattatc cgccgctggc tagcagggca gaatgcaccg atgccttccc
     2041 gcgacgcgtt ggtgaggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     2101 gtttacgttt gggcgcgttt gaaatccgcc gctatcagtc gcaactatgg tggaataaat
     2161 ccgttnnnnn nnnnngcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc
     2221 cctccgcgtg cagatgaagc ggtcagcgtg cgtttcaaag cgccaggact gctgcatatt
     2281 gtcgggcgca acggcggacg taagctgaag aaaatctggc aagagttagg cgttccgccg
     2341 tggctacgtg ataccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg
     2401 gtatttgtga cgcaagaagg tgtggctgta ggtgagaatg gcgtcagttt tgtctggcag
     2461 aaaacgctta gtcaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt
     2521 caatcaggac tcgctcacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt
     2581 atccagacgc agctcgcggg ttgcaccagc ggcctcgacg accagatact ccacattttt
     2641 gcggnnnnnn nnnnctgcaa tttttcgcca tctttcagct caagtgtcag cattaaatga
     2701 tgctggcagg cgagctcaag attatcgtaa tcatcacaat tgattggttg atacgtatca
     2761 ttcattgaca taatcgctca ccagtaagtt tgccgcagcg tatgctgctt tttccctgac
     2821 agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa cgcagcccag
     2881 cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct tgcgtattaa
     2941 ctcacaatat ttttccacat gccctcctgt cagcactctg acttaaccgt ggatgcaagt
     3001 ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc atttctgcaa
     3061 tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga aaggagtttt
     3121 cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga tctcgaccgc
     3181 aaccagtttc tcgatgcctc tctgacgctg gcgcgccatc cttcaga
//
LOCUS       DAWDRC010000474         3218 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11774_length_3218_cov_2.161239, whole genome shotgun sequence.
ACCESSION   DAWDRC010000474 DAWDRC010000000
VERSION     DAWDRC010000474.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11774_length_3218_cov_2.161239"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    709..718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          688 a          899 c          842 g          779 t
ORIGIN      
        1 ttacacgcat cgtcagcgca ggcgcgcccg ccgatttact attgctggct gtcgcgccag
       61 aaaaaatggt cggtttttcc tcttttgatt ttgcccgtca ggcattaatt ccattgccag
      121 agcacattcg ccagttcccc aggctgggac gactcgccgg gcgcgccagc acactctcgc
      181 tggaagggct tatggcgtta catcccgatt tggtcgttga ttgcggcaat acggatgaaa
      241 cctggatctc ccaggcacgg caggttagcg aacagacaca aataccctgg ttattgctta
      301 acgggaaact ggaacaatca gcagaacagt taacaacgct tggcaaaacg ttaggcgaag
      361 agcaccgcgc cgcagaacaa gccaatctcg ccagccgctt cgttggtgaa gctcaggcat
      421 tcgccacctc acccgccgct aatctcagct tttatgctgc gcgcggtcct cgagggctgg
      481 aaacgggctt acagggttcg ttgcataccg aggcggcgga attattaggt ttgcacaacg
      541 tcgcgcaaat agccgatcgc cacggtctga cacaggtttc catggaaaat ctcctgcgct
      601 ggcagccgga tattattctg gttcaggagg ccgttactgc agattttatt cgtcgtgatc
      661 ctctctggca gggcgtgaaa gcggttgcgg aacaacgcat cttattttnn nnnnnnnntg
      721 gatgccccgc cgggaatcaa ccgtcttctg ggattacgca gacttcacgc ctggctggat
      781 cccgccatca atcgccagtt taaaagtgac atacagcatt acgcccaact gttctggcat
      841 tgttcactca gtgacgccga ctatcaaaaa ttggtggcga gctaatgaga accgtaaacg
      901 gatgtatttt gctgctggca gctatcagta tcacttttgc cgccgtaagt ggggcttatc
      961 atctcgatat gcagcaactg ctggcgctca ttcttcgtca agaaaatgtt cctgtgcagg
     1021 aacaaattgt cttctggcaa atccgcgtgc cgcgtattct tgctgcgctg tttctgggcg
     1081 cggcgctggc aggggccgga accacctatc agggaatgtt ccgtaatccc ttagtgtcac
     1141 cggatatttt aggcgtctcc gccggtgccg gacttggtgc ctgcgcggca atcctgtggg
     1201 gattatccat tgtttacatc cagctgtacg cattttgcgg cgggctgatg gtggtcgcgg
     1261 gcgtctggtt gattacgcgt cgggtgaccc gccacgatcc gatcttaacc ctggtgctgg
     1321 taggcattgc gttaggaacg ttgtgcggag cggggatatc gctaataaaa acgctggccg
     1381 atccttacac ccagttaccg tcaattacct tctggttgct gggggggctg tccaccgtga
     1441 ccttacgcga tctctgctat gctgcaccga tcattctcat aggctccttg cccctgtttt
     1501 ttctccgctg gcgcatgaat ttactcacgc tttccgacga cgaagcccgt tcgctgggac
     1561 tgaacgtgac acgtctgcgc tttggattga ttgtctgcgc cacactcatc accgccagca
     1621 cggtagcaat agcaggcata attggttggg tcggcctggt cgttccgcac attgcccgct
     1681 tgttgaccgg tcataaccat cagcagctgt tgcccatggc aatgtgtact ggcgcgattc
     1741 tgctcctgct taccgatacg ctggcacgca gcattggaac aacagaaatc ccacttggca
     1801 ttctgaccgc ttttgttggc gcaccgtttt tcctgttttt gttgttacgc ggaggtcgcc
     1861 agtgactttg cttgccgtcc gtcatgcgtc tctgggttac tcccgccatc cggtgctacg
     1921 cgacgtgtcc ttcaccctgt cgcaaggaac aatttgttgt ctgttaggag ccaatggttg
     1981 cggcaaaacc acactcatgc gctccattct tggcgttata ccgttattaa aaggcgagat
     2041 cttactggat agcctccctg tgcagacact cagccatcgc cagcgcgcgc aggctattgc
     2101 ctgggtgccg caggcgcacg acggcatttt tgcttttagt gtgctggata tggtgttgat
     2161 gggcctggcc cccaccatag gcgctttttc cgttcccggt aagcaagaac gcctgaaagc
     2221 aacggagcaa ctggaaaagc tgggcatttt gcatctggcg acacgacgct ggaatacgct
     2281 tagcggcggt gaacgtcagc tggctttaat cgcccgtgca ctcgtccagc aaccgcgctt
     2341 gttattactg gatgagcctg cctccagcct cgatttcggt catcagatcc aacttctgga
     2401 cacgctggcg cagctcaaaa ataacggcat gacaatgtta atgtcgactc atcatccgct
     2461 ccatgcaaac gccattgccg acagcattat ccaggtagag ccagatggac gagtaacaca
     2521 aggcttaccg accgaacagt taacaacgaa aaaactggct gccctgtacc gggtttctgc
     2581 cgaccagatt caccatcacc tctctgcaat taagcactaa ggaaaaatca tgctcattga
     2641 cgatattgat tttgcagacc tttaccttca gcaactcagg ctggcccatc gcacagaaaa
     2701 aacgccggat cactgggatc aacgggcgga aaaaatggcg gaaaattgcg cctcgcctac
     2761 ggacagctac ctgcaacaac tgacctcaaa aattgacctg caaggcgcgc aaaccttatt
     2821 cgatatgggc tgcggaccgg gtaccgtatc gctggcgctg gcggacaaac tcacgacgat
     2881 ttatggcgtt gattacagtc aggggatgct gaacgttgcc gcccgtcgcg ccgccgcgct
     2941 aaaggcagac aatgtccact ggatccagcg cgcctgggaa gaggactgga gtgacctacc
     3001 acgctgcgat attgccgtgg cttcccgctc cactctggtg gctgatatgc gccaggcaat
     3061 gagcaaactg aataatcagg cacggcttcg cgtctatacg actcatctgg tcagcacctc
     3121 gttcgtctct ccagccattc agcgggcggc tggtcgggaa gtgatcgaat tacccaacta
     3181 tatctttgca ctcaacgtcc tgtatcagat gggaattt
//
LOCUS       DAWDRC010000475         3186 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11892_length_3186_cov_2.613861, whole genome shotgun sequence.
ACCESSION   DAWDRC010000475 DAWDRC010000000
VERSION     DAWDRC010000475.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11892_length_3186_cov_2.613861"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2747..2756
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          853 a          686 c          692 g          945 t
ORIGIN      
        1 cttatataac gaaaacacaa atgtaaaact catcccacag atgaattacc tgatggttgt
       61 tgtggctttg tttttcctta acgccgtcat tttccttttc atgttaatga agtatttcac
      121 taacaaacaa attttaccaa cactcatttt aagccttgca tttttaagtg gccttatcta
      181 tttagttgaa accattgtaa ttatccataa accaatcaac ggcagtacac tgatccagac
      241 aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttca tttgtttaac
      301 ctcgctggcg ctcttttgtt atgggaaaga caacatcctt gacaacaata agaagaaaac
      361 tggcattctg ttgctggcgc tgattccttt tttagttttt ccccttctgg cacacaatct
      421 gagcagttat aacgctgact attctttgta tgtcgtcgat tactgtccgg acaaccatac
      481 tgcgacctgg ggaatcaact atacaaaaat attggtttgt ttgtgggcat ttttactgtt
      541 ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag cattattatg
      601 tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata attacaccat
      661 ctggtatatc agtcgcggga ttgaagtttc cagtaaactg tttgttgtgt cttttctgat
      721 ttataacatt tttcaggaac tgcaactctc cagcaaactg gcagttcatg atgtgctgac
      781 caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt cgcgacctgt
      841 tgttaaggac ttctgtgtca tgctggttga tattaatcag ttcaaacgca tcaatgccca
      901 atggggacat cgtgtgggtg ataaagtgct ggtttcaatt gtcgatatta tccagcaaag
      961 catccgcccc gatgatattt tagcgcgact ggagggtgag gtatttggct tgctatttac
     1021 cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa atgtcgaact
     1081 cctgaccggc tttagtaaca gatatgatgt tcctgaacaa atgaccatca gtattggcac
     1141 ggttttttca acgggtgaca cgcataatat ctcgcttgtc atgacggaag cagataaagc
     1201 cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata tttaagtgca
     1261 aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta ctattacccc
     1321 taaatttccc ctccccagcg gcgcagagat gagtataatt agcgcccctg tgccaggccg
     1381 caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat ttatcatgaa
     1441 attgcaccat agaatgctcc ggcattttat cgccgcaagt gtcattgtgc tgacatcttc
     1501 cttccttatt tttgaacttg tcgccagtga cagagcaatg agtgcctatc tgcgctatat
     1561 cgtgcagaga gcagactcct cctttcttta tgataagtat cagaatcaga gtattgccgc
     1621 gcatgtgatg cgcgctctcg ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc
     1681 catctgcgag gcttttgagt ctgccaataa tacccatggc ttaaacctga ctgcccataa
     1741 ataccctggc ttacgcggca cactacaaac cgcatccact gactgtgaca caattgtgga
     1801 agctgctgca ctattacccg cttttgatca ggcagtggaa ggcaaccgcc accaggatga
     1861 ttacggttca ggtcttggga tggccgaaga gaaatttcac tattatctcg atctcaatga
     1921 ccgctatgtc tatttttatg agccggttaa tgttgagtac tttgcgatga ataactggtc
     1981 cttcctgcag tcaggaagta ttggcatcga tcgcaaagat attgaaaagg tatttaccgg
     2041 gcgtaccgta ttatcgagca tttaccagga tcagcgtact aaacagaacg tgatgagttt
     2101 actgacgccg gtatatgtcg cagggcagct aaaagggatt gtgctgctgg atattaacaa
     2161 aaacaatctg cggaatatct tttacactca tgaccgccct ctcctctggc gttttctcaa
     2221 tgtcacgcta actgatacag attcggggcg cgacattatc atcaaccaga gcgaagataa
     2281 tctgttccag tatgtcagtt acgtccatga cttaccgggc ggcattcgtg tctcgttatc
     2341 cattgatatt ctttacttta tcacgtcttc gtggaaaagc gttctgttct ggattttgac
     2401 ggcgttaatt ttgctgaata tggtgcggat gcacttccgt ttataccaaa atgtgtcgcg
     2461 agaaaatatt agtgatgcga tgactggact gtataatcgc aaaattttaa cccctgaact
     2521 ggagcagcgg ttgcagaaac tggtgcaatc cggttcttcg gtgatgttta ttgctattga
     2581 catggacaag ttaaagcaaa taaatgacac ccttggtcat caggaggggg atttagcgat
     2641 tacgttatta gctcaggcga ttaaacaatc gattcgtaaa agtgattatg ccatccgact
     2701 cggtggcgat gaattctgca tcattcttgt cgattcgacg ccgcaannnn nnnnnnttgc
     2761 agcacaactg cctgaacgta tcgaaaaacg tctgcaacat atcgcgccgc agaaagagat
     2821 cggcttctct tccggtattt acgcgatgaa agaaaacgat acgttacatg atgcgtataa
     2881 agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataatc
     2941 ttctgtggtt gtttgcttgt aatctcagga gcatgaaatg actgaaatgg ctaaaggaag
     3001 cgtgacgcat cagcggttaa tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt
     3061 tacccatgaa gcggtaggta aatgtgaagc agtgtcagaa attcgtggca ccgctttagg
     3121 ccagggggct aaggcactgg tatgcaaagt caaaggcaat ggcgtaaatc aacatgttct
     3181 ggcgat
//
LOCUS       DAWDRC010000476         3179 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_11920_length_3179_cov_2.293214, whole genome shotgun sequence.
ACCESSION   DAWDRC010000476 DAWDRC010000000
VERSION     DAWDRC010000476.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11920_length_3179_cov_2.293214"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2879..2888
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          743 a          947 c          768 g          711 t
ORIGIN      
        1 gatcgagcgt ggtgaagttc gatcccaccg ccaccaccac ctctttggca gcgaatgcac
       61 aagatgccat caacgctgtc gcaatgccca gcgccactaa cccactccgg tgtacagctc
      121 ttgccatttt attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt
      181 tgtgcgacgt aatgccccgg cccgacgcat tgcaacgaga cggctgcaac ctcttcgcca
      241 cgcagatgaa tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg
      301 gacggttcag cgaccggaac tgccgccagt aatttacgcg tataagggtg ctgcggattt
      361 tcaaagaccg cgcgccgtgg accaatttca acaatttgcc cgagatacat caccgccaca
      421 cgatgactaa tccgctctac cacggccata tcgtgggaga taaacagata cgcaatgccg
      481 aaatcacgct ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc
      541 gccgagacgg cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg
      601 cagatgcgct ggcgctgacc gccggaaaac tcatgtgggt aacgccaggc atgttccggt
      661 aacaggccca cgcgctccag caaccacgca acgcgtgcag ccgcttcttt acctggcaat
      721 aaaccgtgta cgcgcagcgg ttcgagaatc gaatcaccga tggtctgacg tgggtccagc
      781 gaagcgtaag ggtcctgaaa aataaactga atatcgcggc gcaatgcctg aagtttaccg
      841 ggtgacaagg tatcgattcg ctgaccgtta aagataattt cgccgccctg cgattcgacc
      901 aggcgcagca acgcccgccc ggtggtggat ttaccgctgc cagactcgcc caccagcgat
      961 agcgtttcgc caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt
     1021 acgcgattca acaaaccgct gcgcaaaggg aaacgggtga ccagattacg cacccgtaaa
     1081 acaggttcgc catccaccac cgttttctgc tcgatgggtg gctcctgttt cgctggatgt
     1141 tcaagcgata tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc
     1201 ggaacagcag ctaacagcgc acgggtataa ggatgttgcg gtgcatgaaa aatctgttcg
     1261 acgctacccg tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct
     1321 gccaccacac ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat
     1381 actttgatta attgcaggat ctgcgcctga atagtgacat ccaacgcggt ggttggctca
     1441 tcggcaatca gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc
     1501 atcccgccag agagttgatg cggataacgt gaaagaatgg tttgtgcctc cggaatgcgt
     1561 acctgatcca gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc
     1621 agacgaattg attctgcaat ctgttcaccc acagtaaata ccgggttcag cgatgtcatc
     1681 ggttcctgaa atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc
     1741 tgctcgctaa gttcaatcac ttcgcgactg cgccgccgca acagcatttt atcgcactgt
     1801 actaaaccgc ccgcctgttc caacagacgc atcaatgcca gcgcagtcac tgacttaccg
     1861 gagccggatt cgccaacaat tgccagcgtc tcaccgcgtt gcaggctaaa agagagattg
     1921 cggaccgcag ctattttctg ctgctcctgc ataaaggcaa tattcagatt ttcaaccgcc
     1981 agcacattac cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta
     2041 gataccggtg gttggcgtat cacctgcgta gccccaggcg cgatacattc cttcggtgtt
     2101 aaacggtagc gcgacattcc ctacatggtc gatagcgatt aagccaccgc taccgccaag
     2161 cgcagggagt ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta
     2221 atccattaac gcggcgatgt catatgccgc cagcgcgcgg atgaagactt cgcccgtgcc
     2281 ggtacaagaa accgccacac tggcgttatt ggcgtagcat ccggcaccca ctaagggact
     2341 atcgccaact cgtccgggta atttattggt cattccgccc gtggacgtgg ctgccgccag
     2401 attgccgtct aaatccaacg ccacggcccc cacggtgccc attttctgtt tttcatccag
     2461 ttgcgcaccg ctatggtcga ggactgttgc cccttcctca cgcgctgcca ttagttgttc
     2521 ataacgcaaa ggcgtggaga aaatctccgg tgagacgcgc tccatgccat gagcaaacgc
     2581 aaaattttct gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagccg
     2641 ggcggcaaga accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt
     2701 gttaccatcc atcacacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc
     2761 aattccggcg ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc
     2821 cagcgcactt tcgcccgctt ccagcatttt ctgcccggtt tcaacaatgg cagacagcnn
     2881 nnnnnnnngc gcctcgatgt agcgtaattc ctgttgcaga ctcatctgcg cgcggctaat
     2941 tgcacctgcg ccgccatgaa ttgcaatgac tgctttgccc atagtattca tccattaagg
     3001 gtggctcgcc acttttgcta taaatatcag aatttttatg aatgactcat tcaatttttg
     3061 aatatagaaa gatggatatg gcatgtaaag gcaggagcca gcgctacgcc cgaggtggca
     3121 tacagtctgt ggcgtttttc tgacataata ggcaaattcg attttgcctc cgcaggagt
//
LOCUS       DAWDRC010000477         3141 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12064_length_3141_cov_1.802009, whole genome shotgun sequence.
ACCESSION   DAWDRC010000477 DAWDRC010000000
VERSION     DAWDRC010000477.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12064_length_3141_cov_1.802009"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1620..1629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2534..2543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2831..2840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          754 a          871 c          861 g          625 t
ORIGIN      
        1 ccaccggatt acaattgccg ctcggcgcaa cctgtgtcat tgagcgccag aacggcagca
       61 aaacgcacga agtagaaagc gaagtcgttg gctttaacgg tcaacggctg tttttaatgc
      121 cgctggagga agtggaaggt gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg
      181 cagaagggct gcaaagcggc aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc
      241 tggacggtag cggtaaaccg ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg
      301 cgctgattac cccgccattt aacccgttgc aacgtacacc gattgaacat gtgctggaca
      361 ccggcgtgcg cccaatcaat gccctgctta ccgtcggacg cgggcagcgt atggggctgt
      421 ttgccgggtc cggcgttggg aaaagtgtgc tgctggggat gatggcacgt tacacccgcg
      481 ccgatgtcat tgtcgtgggt ttgattggtg aacgtgggcg cgaagtaaaa gattttattg
      541 agaatatcct cggtgccgaa ggacgtgcac gctccgtggt gattgccgct ccggcggatg
      601 tttctccgct cctgcgaatg cagggcgccg cctatgccac gcgcattgcc gaagattttc
      661 gcgatcgtgg tcagcatgtg ttgctgatta tggactccct cacccgctat gcgatggccc
      721 agcgtgaaat tgcgctggcg attggcgaac cacctgccac taaaggttat ccaccgtcgg
      781 tgtttgccaa attaccggca ctggtcgagc gtgccggaaa tggcattagc ggcggcggct
      841 cgattaccgc gttttatacc gtgctcaccg aaggcgatga ccagcaggac cccattgccg
      901 actccgcgcg ggcaatcctc gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg
      961 ggcactatcc ggctatcgat atcgaagcgt cgatcagtcg cgcaatgacg gcgttgatca
     1021 gtgagcaaca ttacgcgcga gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta
     1081 accgcgatct ggttagcgtc ggcgcgtatg ccaaaggcag cgatccgatg ctcgacaaag
     1141 ccatcgccct gtggccgcag ctggagagct atttgcaaca aggcattttt gaacgcgcgg
     1201 actgggaagc gtctctccag gggctggagc gtattttccc gacagtgtca taacccagga
     1261 gataacggca gatggcggaa catggtgcgc tggcgaccct gaaagatctg gcagaaaaag
     1321 aggtagagga tgccgctcgc ctgctgggtg aaatgcgtcg cggatgtcag caggcggaag
     1381 aacagctcaa aatgctgatc gattatcaga atgaatatcg caataacctc aatagcgata
     1441 tgagtgccgg gatgaccagc aaccgctgga tcaactatca gcagtttatc cagacgctgg
     1501 aaaaagccat tactcagcat cgccagcaac ttaatcagtg gacgcagaaa gttgacattg
     1561 ccctgaacag ctggcgagaa aagaaacaac gtttgcaggc ctggcagaca ctgcaggaan
     1621 nnnnnnnnnt cgcttagcgc ccttgattac cgccgacgtt gacaccacca cattgcctgg
     1681 cggcaaagcc agcgatgctg cacaagattt tctcgcgttg ttgagcgaag cattagcagg
     1741 cgagacaaca accgacaaag cggcccccca gttgctggtg gcaacagata agcccacgac
     1801 aaaaggcgaa ccgctggtca gcgatattgt ttccgatgcg caacaagcgg atttactgat
     1861 ccctgtggat gaaacacttc ctgtcatcaa cgacgaacaa tccacatcaa caccgttaac
     1921 caccgctcag acgatgacgt tggctgcggt ggctgacaaa aatacgacaa aagacgaaaa
     1981 agcggatgac cttaatgaag acgtcaccgc aagtctgagc gccctttttg cgatgttgcc
     2041 gggttttgac aatacgccca aagtgactga tgcaccgtca accgtgttac cggcagagaa
     2101 accaacgctc ttcacaaaac tgacttctgc gcaactcact acagcacagc ctgatgacgc
     2161 ccccggcaca ccagctcagc cattaacacc gctggtagca gaagcccaga gtaaagcgga
     2221 agtcatcagc acgccttcgc cggtgaccgc tgacgccagc ccgctaatca ctccacacca
     2281 gacacagcca ctgcccaccg tcgccgcgcc tgttttgagt gcaccgctgg gttctcacga
     2341 atggcaacaa tcattaagcc agcatatttc gctgttcacc cgccaggggc aacaaagtgc
     2401 agagttgcgt ctgcacccac aggatttagg tgaagtgcaa atttccctca aagtggatga
     2461 taaccaggcg cagatccaga tgatttcacc gcatcagcac gtacgcgccg ccctggaagc
     2521 agcgctgccg gtannnnnnn nnntcagcag caggccgctt cccagcaaca gcaaagccaa
     2581 cgcacagcaa accatgaacc tctggcgggg gaagacgacg atacgcttcc ggttcccgtc
     2641 tctttacaag ggcgtgtaac aggtaacagc ggcgttgata ttttcgccta acgtcagagg
     2701 tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag gataattagc
     2761 cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg ataagcaaga
     2821 aaagcaagcg nnnnnnnnnn ctcgcggcct gtgccagcgc aggttacagc tactggcatt
     2881 cgcatcaggt tgccgctgac gacaaagcgc agcaacgcgt cgtgccctcc ccggtcttct
     2941 atgcgctgga tacctttacg gtcaatttgg gcgatgcgga tcgcgtgctt tatatcggca
     3001 taaccctgcg actgaaagat gaagcaaccc gctcgcggct gagtgagtat ttgccggaag
     3061 tccgtagtcg cttgctgtta ctgttttcgc gtcaggatgc tgccgtactg gcgacagaag
     3121 aaggcaagaa aaacctgatt g
//
LOCUS       DAWDRC010000478         3121 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12145_length_3121_cov_2.697326, whole genome shotgun sequence.
ACCESSION   DAWDRC010000478 DAWDRC010000000
VERSION     DAWDRC010000478.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12145_length_3121_cov_2.697326"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1120..1129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1351..1360
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2111..2120
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          721 a          842 c          823 g          705 t
ORIGIN      
        1 gtgctaccga tttcacgccg ccggttgagt tggaaggcag ggtgatgccg cggcctaccg
       61 agaggcactc caccagcatc cgccagcctt gcccggccat tttcggcccg ccgatgatgt
      121 aatcgatcgg cacgaacaca tctttaccgc gcgtcggtcc gttctggaac ggtacgttca
      181 gcgggaagtg gcgacgacca atttccacgc ccggcgtggt ggtcgggatc agcgcacagg
      241 taatacctaa atcttctgca ccgccgagta atttctccgg gtcggagagt ttaaacgcca
      301 gaccaagcac ggtcgcaatc ggtgccagcg taatgtagcg tttgttccag gtcagacgca
      361 tccccagcac ctgctggccc tgccattcgc ccatgcagac aatcccggta tccggaatcg
      421 cgcccgcatc ggaacccgct tcggggctgg tcagtgcaaa gcaagggatc tcctgaccac
      481 gcgccagacg cggcagatag tgatttttct gctcgtcagt gccgtaatgt tgcagcagct
      541 ctcccgggcc taatgagttc ggcacgccga cggtaatcgc caggatcccg ctcacgccgg
      601 agagtttttg cagcacgcga gactgggcat aagccgagaa ctccagcccg ccgtactctt
      661 ttttgatgat catcgcgaag aaacgatgct ctttaaggta cgcccacaac tccggcggca
      721 ggtccgccag ctcatgagta atctggaaat cattcgccat ccggcaggct tcttctaccg
      781 gaccgtcgag aaacgcttgc tcttcagcgg tcaggcgcgg ctgcggatag ttatgcagct
      841 ttttccagtc cggcttgccc tggaacaagt cgccctccca ccaggtggtg cccgcatcaa
      901 tcgcttcttt ctcagtgcgc gacatcggcg gcatcacctt acggaaaccg cgaaataccg
      961 gtgcggaaat catcgactta cgcataggcg caaagttaaa tggcacgagg ataatggcca
     1021 gaggcactag tacccacgcc gaccacagac cagcaacgcc gaggacggct gtccaggcga
     1081 gcaaaatcag actgctgata aataagctca cgcggtgatn nnnnnnnnna tagaacaatg
     1141 cgccgagcag gacaaccgta gcgagaatac tcaaaatcat cataacgaaa agccccttac
     1201 ttgtaggagg tctgaccact tgtgatgata tggttgtagt ggatgtaaaa atatttagca
     1261 atgtgtttac aatataatta caacaaagct cacattgttg ctgtttttat ccgcacttca
     1321 ggtcaaaaag tcctggtcat agcacctgcc nnnnnnnnnn cctgcccgta cttctcgctt
     1381 ttggcggtat ccggtacact gcattttgtc tattacattt atgctgaagg atatcctcat
     1441 gtaccaggat cttattcgta acgaactgaa cgaagcggcg gaaacgctgg ctaacttttt
     1501 aaaagatgac gccaatattc acgccattca gcgcgcggcg gtcctgttag cagacagctt
     1561 taaagccggt ggcaaagtgc tttcctgcgg caacggcggc tcccattgcg acgccatgca
     1621 ctttgccgaa gagttgaccg gtcgctaccg tgaaaaccgt ccgggctacc cggcgattgc
     1681 catttctgac gttagtcata tttcctgcgt cggtaacgat tttggtttca atgatatttt
     1741 ctcccgttac gttgaagcgg taggtcgcga aggcgatgta ctgctgggga tctccacttc
     1801 cggtaactct gcaaacgtga tcaaagcgat cgcggcggcg cgtgagaagg ggatgaaagt
     1861 gatcaccctg accggtaaag acggcggcaa aatggctggc acggcggata ttgaaattcg
     1921 cgttccgcac tttggttatg ccgaccgcat tcaggagatc cacattaaag tgatccatat
     1981 cctgatccag ttaattgaaa aagagatggt taagtaagtc tggcgtaggc cggataaggc
     2041 gtttacgccg catccggcat ttgtgctctg atgcctgatg cgacgctgac gcgtcttatc
     2101 atgcctacaa nnnnnnnnnn tgcctacaat ctgcacccga accgtaggcc gaataatgcg
     2161 ttcacgccac atccgacctg aaaattctta aatcaatctt cgccgggggc catgcgctcc
     2221 cgctgttgtg gaggttaccc atgtgcgaat tgctcgggat gagcgccaac gtccctaccg
     2281 atatctgctt tagtttcacc gggcttgtac agcgtggtgg tggaaccggg ccacataaag
     2341 atggctgggg cattaccttt tacgaaggta aaggctgtcg cacatttaaa gatccgcaac
     2401 ccagctttaa ttcccccatc gccaaacttg tccaggacta cccgataaaa tcctgttcgg
     2461 tggtggctca tattcgccag gctaatcggg gcgaggtggc gctggaaaat actcacccgt
     2521 ttacccgcga gttatggggg cgtaactgga cttatgccca taatggacaa ctgacgggct
     2581 acaaatcact ggaaaccggc aacttccgcc cggtcggtga aaccgatagc gaaaaagcct
     2641 tttgctggct cctgcataaa ttaacgcagc gttacccgcg cacaccgggc aacatggcgg
     2701 cagtgtttaa atatatcgcc tcactggtgg atgaactgcg gcagaagggc gttttcaaca
     2761 tgctactttc ggatgggcgc tatgtaatgg cgtattgctc gactaattta cactggatca
     2821 cccgccgcgc gccgtttggc gtggcaacgt tgctggatca ggatgtggaa atcgacttca
     2881 gctcgcagac cacaccgaat gatgtggtca cggtgattgc aacacagccg ctgacgggca
     2941 atgaaacctg gcaaaagatt atgccaggcg aatggcgctt attttgcctc ggggagcgtg
     3001 tagtttgatg ccagctgtgg ttgcacaact tcgtggctta acggcttgct gaccacgtaa
     3061 cggccattga ccacagaaac ggtcggtggc ttacgggttt gctcaaagta gtcgtaacct
     3121 g
//
LOCUS       DAWDRC010000479         3105 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12199_length_3105_cov_2.524262, whole genome shotgun sequence.
ACCESSION   DAWDRC010000479 DAWDRC010000000
VERSION     DAWDRC010000479.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12199_length_3105_cov_2.524262"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    398..407
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    843..852
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2260..2269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          751 a          864 c          772 g          688 t
ORIGIN      
        1 ggctgacaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
       61 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
      121 tgctggcctt tgattttgcg ggtgttcatc acagcgatat ggtgacactg ctggcggagt
      181 acggtattgc cctgcgcgcc gggcagcatt gcgctcagcc gctactggca gaattaggcg
      241 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
      301 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
      361 cgccggacat ccgttcggca caaccgtaac cgcagaannn nnnnnnntac cttcgcaccg
      421 ttgacgcaat gggaagataa atatcgccag ttgatcatgc tgggaaaaca gcttccggct
      481 ttgccagagg agttaaaagc gcaggctaaa gagattgccg gatgcgaaaa ccgcgtctgg
      541 ctgggatata ccgtggctga aaacggcaag atgcatttct ttggcgacag cgaagggcgc
      601 attgtgcgcg gcctgttggc ggtgttgttg actgccgttg aggggaaaac cgccgccgaa
      661 ttgcaggcac agtcgccgct ggcattgttt gatgagctgg gattacgtgc gcaacttagc
      721 gcctcacgca gccaggggtt gaatgcgtta agcgaggcga ttatcgctgc ggcgaagcag
      781 gtttaagcac agaaccaggt cggataagac gttcgcgtcg catccgacct gattgtttac
      841 ccnnnnnnnn nntttacccc tgacgcgccg ctttcgccat catcttcttc agcgcatgag
      901 aaaccgcaac aaagccaaaa gtggcggtca ccatcgtcgc cgcgccaaat cctgaggcac
      961 aatccatccg ctttggccct tccgccgtgg ctttcatcgc acagacggtg ccgtctgact
     1021 gcgggtacac cagcgcttcg gtagagaaca cgcaatccac gccgagctta cctttactgt
     1081 ttttcactac gccaaaatcg cttttcaggc gctcgcgcaa cttcgccgcc aacgggtcct
     1141 gaatcgtttt cgccagatcg gtaacctgaa tctgcgtcgg gtcaatctgc ccaccagcgc
     1201 caccggtcgt gaccagcggg attttattgc gccgacaata agcaatcagc gccgctttgg
     1261 gccgtacact atcaatagca tcaatcacgt acgaataacc ggcattcata tattgcgcta
     1321 cgttatccgg cgtcacgaag tcatccacca ccattacacg gcactccggg ttaatctggc
     1381 gaatacgctc cgccatgact tccgcttttg ccagcccgac gttatcgcgc agagcgtgaa
     1441 tttgccgatt ggtattggtg acgcacacat catccatatc gataagcgtg attgcgccaa
     1501 tccccgtgcg cgctagcgct tccgccgccc aggaaccgac accaccgata ccaaccacac
     1561 aaatatgcgc gtcagcaaac agttgcaatg ctttttcacc atacagacgc gctgtgccac
     1621 caaaacgctg acgccatgca tcactaatta ccacagacat agcacctcag aattaaaaag
     1681 ggtgaggtta cccctcaccc tgtcatatcc gtaaaaacgg catacagaat atcacatcag
     1741 ccgctaaaga cgttacctgc gcccggggcg gttttcagca cccagacacg tccatagtgg
     1801 ttgtaccaac cagcgcggtg tccggcttcc ggcccgatcc cttgatagat atcgaagtgt
     1861 tggcctttga ttgcgccacc gacatccagc gccaccatca gacgcaattc gtactgaccg
     1921 ttaaatttgc cgttattatc cagcagcggc acttctgcca gcaaggtagt acctggcgga
     1981 ataattgaac gatcggaggc aacagaagca cggccaacca gcggcaccgc acttgcccct
     2041 ttcaccggtg caaaagattg cggtttaaag aagacgaaag acgggttctg ttccagcagc
     2101 tcgcgaacct cagcttcact gtgtgtttcg ccccagtgac gaatcgcctg catcgacata
     2161 tcttcttttt tcacttcgcc acggtcgatc aacaccttac caatgctgcg ataggcatga
     2221 ccgtttttcc ctgcatagct gaaaaagtta agcggactgn nnnnnnnnna tcaccaaagt
     2281 cgatataccc gctaccctgc acatccataa tgaagttatc catcagggag ttactgtaag
     2341 cgagaatata tttatcactc agagctcctg cgtagatctc cgcacgagac ggcagacgac
     2401 cacgttttgg cggcatacgg taaatcggat actggaactc gccctgacgg gtatggcgcg
     2461 cctgaattac cggcgtgtaa tagcctgtaa actgcacgtt accatagttg tcgacacctt
     2521 ccatctgcca ggcatcaatg ccgaactggc gcatattgcg ggtatcaccg cctgcgcgca
     2581 gccactcttg caccgcgtta taaacattac tctggttgcc atacagacgc ggtgacgaat
     2641 tacggatatg gttaatttgc tcggcaaaat caccggcatt aatcggcgcg ccaacggcat
     2701 ctggctggtt caccagagag aaaggctggg taaatttccc gtctttatat tgctgtccgc
     2761 gatcggttgg tttggaagag caggcggcaa gcattgccac aaccgtgccc ataagaaggt
     2821 actttaccca acgtcctttc attgttctct tcttcaggtt aaaaaataag gcgcaacgaa
     2881 gataacaaac caccgcgttt aatgaaatga gcagcctctc cctgacgcaa attttgcaca
     2941 aaaaataggc tttagtgatt tgtttttgtt caaaatcatg ccgaatccgt gatcggggta
     3001 aaaaaaaggt tgcatgaaaa cgcgagcaga gtatagtgcg catccacgga cgcggggtgg
     3061 agcagcctgg tagctcgtcg ggctcataac ccgaaggtcg tcggt
//
LOCUS       DAWDRC010000480         3092 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12245_length_3092_cov_2.263089, whole genome shotgun sequence.
ACCESSION   DAWDRC010000480 DAWDRC010000000
VERSION     DAWDRC010000480.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12245_length_3092_cov_2.263089"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          692 a          765 c          779 g          856 t
ORIGIN      
        1 aatcgtcccc aacggtcgat actcaataac cgcctgctga ttttccacca gcgtaggttc
       61 cgctttcagc attgctggac catgttctgc ataccagtca cacaaattcg ccgatttcgc
      121 cacttcagcg cgcgcctggt tgattggttt gcccatttca cgggtgatca tttgcgccat
      181 ttcttcgcta cgggcgcgca gagccttacc gatatcacgc agtttttcag cacgataatc
      241 tatatttgcc tcgcgccagt cgcgaaagcc tgctgctgcc agctgaagcg cgttttcgat
      301 atcgtcaaca ccagcccacg gcagcacaga aagttgttca ccggtggcag gatttatcga
      361 aattgcatga gttgccggag taatggtcat cggggtatct cctttatgag tcatggtatg
      421 aagatacgca gatttactct tgctttaaaa tgaataatat taagccactt attcacgaat
      481 cgagaatgct atggatctga cccaactgga gatgttcaac gcggttgccg aagctggcag
      541 cattacccag gctgcagcaa aagtgcatcg cgtgccgtcg aatctcacta cccgtctacg
      601 ccagctggaa acagaactgg gggttgatct gtttattcgc gagaatcagc gtttacgtct
      661 ctcgcctgcc gggcataact ttttacgcta tagccaacaa attctcacgt tagtggatga
      721 agcgcggagc gttgtcgctg gcgatgagcc gcaaggttta ttttctctgg gatcgctgga
      781 aagcaccgct gcagtgcgca ttccggccac gctggcggaa tttaaccgtc gttatcccaa
      841 aattcagttt tcactttcca ccggcccttc cggcactatg ctggaaggtg tgctggaggg
      901 aaaactgaat gcggcgttta ttgatgggcc cattaaccat actgccattg acgggatacc
      961 ggtataccgc gaggaactga tgatcgtcac gccacaagga catgcgccag taacccgtgc
     1021 cagtcaggtg aatggcagta acatttatgc cttccgcgcc aattgttcgt atcgtcgcca
     1081 cttcgaaagc tggtttcatg ctgacggtgc cgctccggga actattcacg agatggagtc
     1141 ttatcacggg atgttggcct gtgtggtcgc aggagcaggc attgcgctta ttccacgctc
     1201 tatgctggaa agtatgccgg ggcatcacca ggttgaagcg tggccgttag ctgagcaatg
     1261 gcgttggtta acaacctggc tggtctggcg tcgtggagcg aaaacgcgtc cgcttgaggc
     1321 atttattcaa ctgctggatg cgcctgactc ggcaaaacag ggttaccaat gagctatttt
     1381 tgatagttca tacggctaat atgctctata tagtgatgtt ccgatgactt atgactatat
     1441 ggggcaaata tggttacgcc agtaagcatc agcaattaca tatctcttcc tgatgatttt
     1501 cccgttcgta atattgcacc tcaagtaaaa gaagaacagg cccccttcct gatcgtcatt
     1561 aaaatgtgat gttaacaact ctctgatgcg tgacagaaca accgtcagag agatggaaat
     1621 gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga tcatttcatc
     1681 actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc ccgcaaagta
     1741 gcgtggctac gggtcgttac gctggcagtc gccgccttta tcttcaatac caccgaattt
     1801 gtccctgttg gcctgctctc tgacattgcg catagttttc acatgcaaac cgctcaggtc
     1861 ggcatcatgt tgacaattta tgcatgggta gtggcgctaa tgtcattgcc ttttatgtta
     1921 atgaccagcc aggttgaacg ccgcaaatta ctgatctgcc tgtttgtggt gtttattgcc
     1981 agccacgtac tgtcgttttt atcgtggagt tttaccgttc tggtgatcag tcgcattggt
     2041 gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat ccgtatggct
     2101 ccggccggga agcgagcaca ggcattgagt ttaattgcca ccggtacagc actggcaatg
     2161 gtattaggtt tacctctcgg gcgcattgtg gggcagtatt ttggctggcg aatgaccttc
     2221 ttcgcgattg gtatcggggc gcttattacc cttttgtgcc tgattaagtt acttcccttg
     2281 ctgcccagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg ccgcccggca
     2341 ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac ggcatacagc
     2401 tatatcgagc cttttgtaca aaacattgcg ggattcagcg ccaactttgc cacggcatta
     2461 ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa actgggtaat
     2521 cagtatgcgt ctgcgttggt gagtacagcg attgcgctat tgctggtgtg cctggcactg
     2581 ctgctacctg cggcgaacag tgaaatacac ctcgggatgc tgagtatttt ctgggggatc
     2641 gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc accagatgcc
     2701 accgacgtcg cgatggcgct attctccggg atatttaata ttggaatcgg cgcgggtgcg
     2761 ttggtaggta atcaggtgag tctgcactgg tcaatgtcga tgattggtta tgtaggcgcg
     2821 gtgcctgctt ttgctgcgtt aatatggtca atcatcattt ttcgccgctg gccagtgaca
     2881 ctcgaagaac agacgcaata gtcgaaaggc ccattcgggc cttttttaat gatatgtttt
     2941 aatgatttcc aggatgccgt taataataaa ctgcacgccc atacatacca gcaggaatcc
     3001 catcagacgg gagatcgctt caataccccc cttgcccacc agccgcataa ttgcgccaga
     3061 gctgcgtaga cttccccaca aaataaccgc ca
//
LOCUS       DAWDRC010000481         3091 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12252_length_3091_cov_2.310606, whole genome shotgun sequence.
ACCESSION   DAWDRC010000481 DAWDRC010000000
VERSION     DAWDRC010000481.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12252_length_3091_cov_2.310606"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1101..1110
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          839 a          732 c          755 g          755 t
ORIGIN      
        1 agttgctctg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaatcatca
       61 tctgcactca ctgcgtgacg taagcggatg gtgtggccgg aaacctcata gtgaccgccc
      121 accagttggc ctgcatcgct ttgtagcgta cgcgcggcat cagcaataag attcagatac
      181 tcagactctt ccggggcctt cgccagcata aaagaggagg atgctcgcgt atgcagcaac
      241 tgctccagcg caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca
      301 atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc
      361 catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt
      421 ataccgtaac cggcactgca gcacacgtag aaagcaacga caatactcct ggcatgggcg
      481 ttaaagctca caggatggag attcttttct tcactggcct aaaaagctga tattctgtaa
      541 agagttacac ggtaacattg agatcgctat gaaatatcaa caacttgaaa atcttgaaag
      601 cggttggaaa tggaagtatc tggtgaagaa gcaccgcgaa ggggagttaa tcacccgtta
      661 catagaagcc agtgccgccc aggaagccgt cgatgagttg ctctcgctgg aaaatgaacc
      721 cgtgctggta aatggttgga ttgacaagca tatgaatccg gaactggtca atcgcatgaa
      781 gcagaccatt cgggccagac ggaagcgcca ttttaatgca gaacaccagc atacgcgcaa
      841 aaaatcgatc gatctggaat ttatcgtctg gcaccgtctg gctggccttg cgcagcgtcg
      901 cggtaaaacg ctttctgaaa cgattgttca gctgattgaa gatgcggaaa acaaagagaa
      961 atacgcgaat aaaatgtctt ctttgaagca ggatctgcag gcattgctgg gtaaggaata
     1021 actgtcaaaa gtcagttcaa tttacgcaag gcgaaaaaaa accccgcagc agcggggttt
     1081 tttaccagac gagaacttaa nnnnnnnnnn tttcacgttg tcacaggtgt tgccagtaac
     1141 cgggttggat tcgcccatac cacgtgcgga gattttgtct gccgggatac ctttagagat
     1201 caggtaatca acaacggact gagcacgacg ctcggacaga gcctggttat aagcgtcaga
     1261 accgatgcgg tcagtgtaac ccagaacaac tacggaaccg tctttcggat ccaggttgct
     1321 cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg ttgctttgtt
     1381 gaaggtgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt ccggtgccgg
     1441 agctggagcc ggagcaacta ctggagctac ttcgccctga ccgaaacggt aggaaacacc
     1501 caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac cgatgttgtt
     1561 ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcatact caacaccgcc
     1621 agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg atttagtgtc
     1681 tgcacgccat accataccac ccagacgagt gtatacgtcc agatcgtcag tgattgggta
     1741 acccagttta gcggtcagct gaacgccctg agctttatat gcaccgtttt caacgctgcc
     1801 tttgtatggc atacgaccta accagtcata acccatttca aagccaacat acgggttaac
     1861 ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcggac cattgttgtc
     1921 gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt accaggtgtt
     1981 atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg caatcgcgat
     2041 agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg aatatctcca
     2101 acgagataac acggttaaat ccttcaccgg ggatctgctc aatattaact ctaccgatat
     2161 cttcggctta tgccgagcac ccctggcgat gtaaagtcta caacgtagtt gaaaacttac
     2221 aagtgtgaac tccgtcaggc atatgaaaaa aaagtcttgt ataaggtacg tttaatcttt
     2281 tttgtcagcg acaatttaca gaagaaaatc gcggaaaccg cttcagacaa gcctccgcaa
     2341 ggaaaattag tcacgactga aagcattggc tgggcgacaa aaaaagttcc aggattaatc
     2401 ctaaatttac ttaatgatac aaattagagt gaatttttag cccggaaagt tgtctcgttg
     2461 cgtgagagga tgcgcttacc ggacgcataa taaaccccat agcgttacct tcatttgccg
     2521 catcaacaag ttcagcatgc tcttctgcag tcaaatcatc tgccaaccaa ccgatcacca
     2581 cgctgtaatt gcccgtgcgt aaagcgcgaa ccattgactc cacggtgtgg caaggggaga
     2641 gctggctaat ctgcattact ttcgttaagg gtagcccaga tgcctgaacc cattcgcgac
     2701 tcagtttttg ttgcggtgtt aaccagagtt gccagcgcga ttgctgaccg agttgctgta
     2761 acaatggcaa cagtagaagt tgcgtcatca tgggctgatc ttcgcgatag acaacttcac
     2821 tgataagccc ggctgtagtg ttttccgtag agacacgcgc aattttactt gctgcggatg
     2881 agaacgacga agaacgatgt gcatagcctg aagtgtacat aatcaatcca gcccctgtga
     2941 gttactgtat gaatgtacag tacatccagt gacgacaaag atcaactcta ttttcggaaa
     3001 gagcctcgca aattttgtcg ttggtgacgg gaaaacataa attaatcttg ccccttaaga
     3061 ataagttgcc tattttcgta gttaacggat c
//
LOCUS       DAWDRC010000482         3088 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12263_length_3088_cov_1.992746, whole genome shotgun sequence.
ACCESSION   DAWDRC010000482 DAWDRC010000000
VERSION     DAWDRC010000482.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12263_length_3088_cov_1.992746"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1874..1883
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2277..2286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          752 a          800 c          784 g          732 t
ORIGIN      
        1 cggtgcattg attgattctc aggcggcaat tgaagcgctg aaaaatcaga aaattggttc
       61 gttgggtatg gacgtgtatg agaacgaacg cgatctgttc tttgaagata aatccaacga
      121 cgtgatccag gatgacgtat tccgtcgctt gtctgcctgc cacaacgtgc tgtttaccgg
      181 gcaccaggca ttcctgacag cagaagctct gaccagtatt tctcagacta cgctgcaaaa
      241 cttaagcaat ctggaaaaag gcgaaacctg cccgaacgaa ctggtttaat cttgccgctc
      301 ccctgcattc caggggagct tattcagata atccccaaag acctttcatc ctctattctt
      361 aaaatagtcc tgaatcagaa acagtaattg agaaccacaa tgaagaaagt agccgcgctt
      421 gttgcgctaa gcctgctgat ggcgggatgt gtaagtagtg acaaaattgc tgtaacgcca
      481 gaacagctac agcatcatcg ctttgtgctg gaaagcgtaa acggtaagcc cgtgaccagc
      541 gataaaaatc cgccagaaat cagctttggt gaaaagatga tgatttccgg cagcatgtgt
      601 aaccgcttta gcggtgaagg caaactgtct aatggtgaac tgaccgccaa agggctggca
      661 atgacccgta tgatgtgcgt taacccgcag cttaatgaac tcgataacac cattagcgaa
      721 atgctgaaag aaggtgcaca agtggatctg accgcgaacc agttaacgct ggcgaccgcg
      781 aaacagacat taacttataa gctggcggat ttaatgaatt aatagctgcc acagctcccg
      841 gcggcaagtg actgttcgct acagcgtttg ccgttgggta atgcacacat cccaatcgcc
      901 gtaccatcca gttggcgggc aacagaaagc gaaccgccga tcattgcaca atttgcttct
      961 ccactactgg acatcgacgc ttttaaacct ggcgctacgt gcgccgcagt cgcctgctga
     1021 acaggttcac tactacacgc cgacaacaat aaagcggcac accctaccca aaacgctgct
     1081 cgcatctttt cttcctctga tcttcaagcc aaacgacacc gccataaata ataggcagca
     1141 cagagggcgg cgtcgagagc tgtcctgcgc gttgccccgc atttttactt ttttatggct
     1201 atttttttgc cctctgtttg atcaaaacat tcattacgct gatgtggggg acacaaaagc
     1261 gaaaatgcag aagaaagcca tttgctaaaa ttgaacgatt actactgggc gcgcagcaat
     1321 ttcgtgcgcc cctcatttgc gcaatgtaag ggtgtcatat gattactatt gacggtaatg
     1381 gcgcggttgc ttcggtggcg tttcgcacca gtgaagttat cgccatctac cctattaccc
     1441 ccagttccac gatggcagaa caggctgatg catgggccgg aaacggcttg aaaaacgttt
     1501 ggggagacac accacgcgta gttgaaatgc agtcggaagc gggtgctatc gctaccgtgc
     1561 atggtgcttt gcaaactggt gccctctcaa catcgtttac gtcatcgcag ggtttgctgc
     1621 tgatgatccc aacgctgtac aaactggcag gcgaactgac gccgtttgtc ctgcatgttg
     1681 cggcacgtac cgttgccaca catgcactct ctatttttgg cgatcattcc gacgttatgg
     1741 cggtgcgcca gacgggttgc gcgatgttgt gtgcagcaag cgtccaggaa gcgcaagact
     1801 ttgctctcat ttcgcacatc gcgacgctga aaagtcgcgt gccatttatt catttctttg
     1861 atggtttccg cacnnnnnnn nnngatgccg caggcagaaa ttgatgctca tcgcgcccgg
     1921 gcactcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     1981 cagtctcgcg aagccaccaa cccatggtac aacgcggtct atgaccatgt tgaacaggcg
     2041 atgaatgatt tcgctgccgc gacaggtcgt cagtatcagc cgtttgagta ttacgggcat
     2101 ccgcaagcgg aacgggtaat tatcctgatg ggctcagcca ttggcacatg tgaagaagtg
     2161 gttgatgaat tgctgacccg tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc
     2221 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtgcgcaa cgtggcnnnn
     2281 nnnnnnctgt tcggatagtg gcggtgctgg atcgcaccaa agagccaggt gcgcaagcgg
     2341 aacccctgta cctggatgtg atgaccgcac tggcagaagc ctttaataat ggcgagcgtg
     2401 aaaccctgcc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa tttggcccgg
     2461 actgtgtact ggcggtattt gccgagctca acgcggctaa accgaaagcg cgctttacgg
     2521 ttggtattta cgatgatgta accaatctgt cactgccgtt gccggaaaac accctaccaa
     2581 actcggcgaa actggaagcc ttgttttatg gccttggtag tgatggcagc gtttccgcga
     2641 ccaaaaacaa tatcaagatt atcggaaatt ccacgccgtg gtacgcacag ggctattttg
     2701 tttacgactc caaaaaggcg ggcggcctga cggtttctca ccttcgcgtg agcgaacagc
     2761 cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac cagttgcagt
     2821 ttctcgataa atatcagatg gctgagcgtt taaaacctgg cggcattttc ctgctcaaca
     2881 cgccgtacag cgcagctgaa gtgtggtcgc gcttgccgca ggaagttcag gccgtgctaa
     2941 accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc
     3001 tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg cagattctgc
     3061 ctggcgatag cgccctggca gaattgca
//
LOCUS       DAWDRC010000483         3081 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12300_length_3081_cov_1.857237, whole genome shotgun sequence.
ACCESSION   DAWDRC010000483 DAWDRC010000000
VERSION     DAWDRC010000483.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12300_length_3081_cov_1.857237"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    298..307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          679 a          769 c          768 g          855 t
ORIGIN      
        1 tgtggaatag caataccatt gccgaggaac gttgaggttt gctgttcgcg cgccagcatg
       61 ccattgacgt agccttcagc tacattaccg gcctgcacca gcgccgcagc gacctggcga
      121 atcgcctctt ctttgtctcc ggccttttcg cccggatgga tgtcctgtac ggataactgg
      181 aacatagttc tcctctcttg ctgaattgaa acgattcagc ttctatgaga aaaaaagcgc
      241 caacctggct tagggttaaa gacaagatcg cgctgaaacg tttcaaggaa gcataatnnn
      301 nnnnnnnttt tgtttcagca cgcaaggaaa gccgtaaagt tatgagcaaa agtttgatgt
      361 gctgcacaat tttttgtcat ttttcctaat tgctgatggg aaaatccgtt atcagcaatt
      421 tcatttcagc agcgtcagca gccaatcagc aacagtgaaa tggcattttg attattcgtg
      481 gcgcttttga tttcgctgtc tgtttatttt ctgacaagca gcgtaaactc cgcgtcttcc
      541 tcttccagtg atcgaccagc atgcataact cccccgcagt caccagcgcg aaatcgtttg
      601 acctgacctc gatggcgttt ttaatcgttg cctttctcac cggtattgcg ggcgctctgc
      661 aaaccccgac actcagtatt tttcttactg atgaagtaca tgcccgtccg gcgatggtgg
      721 gattcttctt taccggcagc gcagtcattg ggattctggt gagtcagttt ctcgccgggc
      781 gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt ttgctgcctg ttaggcgtgc
      841 tggcctgcac cctttttgcc tggaatcgca actactttgt tttgctattc gttggcgtct
      901 ttcttagcag ctttggctcg accgctaacc cgcaaatgtt tgcccttgcc cgtgaacatg
      961 ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt tttacgcgct caggtttcac
     1021 tggcatgggt cattggccca ccgctggctt atgccttagc gatgggtttc agctttacgg
     1081 taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg cgtgatggtg tggcggtttt
     1141 taccgtcgat gcaaaaagag cttccgctgg cgaccggcac ggttgaagcg ccgcgccgta
     1201 accgtcgcga tacgttgctg ctgtttgtca tttgtacatt gatgtggggc tcgaacagcc
     1261 tgtacatcat caacatgccg ctatttatta tcaacgaact gcatcttccc gagaaactgg
     1321 ccggtgtgat gatggggacc gccgccgggc tggaaatccc gaccatgttg atcgccggat
     1381 attttgccaa acgtctgggt aagcgcttct taatgcgcgt tgctgccgtg ggtggcgtct
     1441 gtttttacgc aggaatgctg atggcgcatt cacctgtcat tctgttgggc ttgcagctgc
     1501 taaatgctat ttttattggc attctgggcg gcatcgggat gctctatttt caggatttga
     1561 tgcccggtca ggcaggttca gccaccacgc tctataccaa cacgtcgcgc gtgggctgga
     1621 tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg gaattatcac gctgtgttct
     1681 ggtttgcgat ggtgatgatt atcgccactc tgttttgctt actgcgaatt aaagatgttt
     1741 aaggcgcggt cagcatttcc agatcgataa gccaggtcat tgcctgctga cgcgagttac
     1801 cgcacatttc agcgctggct tgtaagcctg cacagacttt cggtcgcagt ggcgaggtga
     1861 aaattctaca gcgttgctgt tcatcaagct gaatgcaggg cgtattggcg ggcttgccat
     1921 ctggcatacc gggaataggg ctggaaattg aaggggcagt gcaacacgcc ccgcaacccg
     1981 gacggcattc catacgttgt tctcttaagg ttaaccaccg gattgatgag cgcgcacagt
     2041 accacctttt ttgcaccagc aaaagtgcga ataccacttg cccgaaaggc ccgtcgcgag
     2101 tactttgtcg cgatattttt gacatttttg actacaggaa tttttcgatg ccaagagcga
     2161 acgaaattaa aaaaggtatg gtactgaatt acaacggcaa actgctgctg gttaaggaca
     2221 ttgatattca gtcgcccact gcccgcggtg ccgctacgct gtacaaaatg cgtttttctg
     2281 atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg tgatgatatc gttgacaccg
     2341 tgacgctgac ccgccgttac gttgacttct cctatgtcga tggcaacgaa tatgtcttta
     2401 tggataaaga agactatacc ccgtatacct tcaccaaaga tcagattgaa gaagagttgc
     2461 tgtttatgcc tgagggcggc atgccggaca tgcaggtgct gacctgggac ggtcaattgc
     2521 tggcgcttga gctgccgcag accgttgatc tggaaatcgt tgaaactgca ccaggcatca
     2581 aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt gagcactggt ctggtgattc
     2641 aggtaccaga atacttaagc ccgggcgaaa aaattcgtat ccatatcgaa gaacgccgtt
     2701 atatgggtcg tgctgactaa cttcagccgt atgcagaaaa gggatagctc aggctgtccc
     2761 ttttttaatt tattatacca atcttctatt ttgcgcttcc tgaacacctt acgcccgcaa
     2821 ttttcactcg ttgattgatc gccctcacac ttcatcgcat taacaatcca gaccaatttc
     2881 aattgttgtc atataacttt acactgttat tgttaattaa tcgttactaa gacgtgactc
     2941 ctatgaatac aatcgcctcc gttacgctcc cgcatcatgt acacgctcca cgctacgatc
     3001 gacagcagtt gcaatcacgt atcgttcatt ttggctttgg agcctttcac cgcgcacatc
     3061 aggcattact gaccgatcgt g
//
LOCUS       DAWDRC010000484         3076 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12318_length_3076_cov_2.163191, whole genome shotgun sequence.
ACCESSION   DAWDRC010000484 DAWDRC010000000
VERSION     DAWDRC010000484.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12318_length_3076_cov_2.163191"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    546..555
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    806..815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2456..2465
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          724 a          750 c          913 g          659 t
ORIGIN      
        1 tgtggagtgg gatgacgcgc aaggtacgct gaaagcctgg cgtcggctgc aaatcggtca
       61 gttgacggta aaagtgcaac cgctggcgaa accctcggaa gacgagttgc atcaggcgat
      121 gcttaacggc attcgtgata aaggtttaag cgtgctcaac tggacggcgg aagcggaaca
      181 gctacgcttg cgtttgttat gcgccgcaaa gtggttgccg gaatatgact ggccagcggt
      241 tgatgatgaa agtttgttgg cgacgctgga aacgtggcta ctgccgcata tgacaggcgt
      301 acattcgcta cgcggcctga aatcactcga tatttatcag gcactgcgtg gattacttga
      361 ttgggtaatg cagcaacgtc tggatagtga attgcctgcg cattacactg tgccgacggg
      421 aagccggatc gccattcgtt accatgaaga taacccgccc gcgctggcgg tgagaatgca
      481 ggagatgttt ggtgaggcct ctaatccgac tatcgcccag gggcgcgtac cgttggtgct
      541 ggagtnnnnn nnnnnaaggc cgctgcaaat cacgcgtgat ttgagcgcct tctggaaagg
      601 agcgtaccgt gaggtgcaaa aagagatgaa agggcgttat cccaaacatg tctggccgga
      661 cgacccggca aacaccgcac cgacgcgacg aacgaaaaag tattcgtgaa gaccaaagca
      721 aatttgagag atatcttctt ctgtcttgca acagaagaac agaaaatcgg gcttttgcgc
      781 ctgaatattg cggagaaaaa gcatgnnnnn nnnnnccgcg agccaattgg acgcaaaggg
      841 aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga tgacgatgat
      901 tacgacgatt atgatgacta tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa
      961 ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat ggctgctgct aaaactggct
     1021 atcgtttttg ccgtgctgat cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc
     1081 cgtattgatg gcaaggtctg gcaattgccc gcggcagttt atggccgaat ggtcaacctt
     1141 gagccagaca tgaccatcag caagaacgag atggtgaagc tgctggaggc gacccagtat
     1201 cgccaggtgt cgaaaatgac ccgtcctggc gagtttaccg tacaggccaa cagcattgag
     1261 atgatccgcc gtccgtttga tttcccggac agcaaagaag ggcaggtgcg cgcgcgtctg
     1321 acctttgatg gcgatcatct ggcgacgatc gtcaatatgg agaacaaccg tcagttcggt
     1381 ttcttccgtc ttgatccgcg cctgatcacc atgatttctt cgccaaacgg tgagcagcgt
     1441 ctgtttgtgc cgcgcagtgg tttcccggat ttgctggtgg atactttgct ggcgacagaa
     1501 gaccgtcatt tctacgagca tgatgggatc agtctctact caatcggacg tgcggtgctg
     1561 gcaaacctga ctgccggacg cacggtgcag ggggcgagta cgctgacgca acagttggtt
     1621 aaaaacctgt tcctctccag cgagcgttct tactggcgta aagcgaacga agcctacatg
     1681 gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc ttgagctgta tatgaacgag
     1741 gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct tcccgctggc gagcttgtat
     1801 tactttggtc gcccggtaga agaattgagc ctcgatcagc aggcgctgtt ggtgggtatg
     1861 gtgaaagggg cgtccatcta caacccgtgg cgtaacccga aactggcgct ggagcgacgt
     1921 aacctggtac tacgtttgct gcaacagcaa caaattattg atcaagaact ctacgacatg
     1981 ctgagtgcgc gtccgctggg tgttcagccg cgcggtgggg tgatctcccc tcagccagcc
     2041 tttatgcagc tggtgcgtca ggagctgcag gcgaaattag gcgataaggt aaaagatctc
     2101 tccggcgtga agatcttcac tacctttgac tcggtggccc aggacgcggc agaaaaagcc
     2161 gccgtggaag gcattccggc actgaaaaaa cagcgtaagt tgagcgatct tgaaaccgcg
     2221 attgtggttg ttgaccgctt tagcggtgaa gttcgtgcga tggtcggtgg ttctgagccg
     2281 cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa
     2341 ccagcgactt atctaacggc cttaagccag ccgaaaatct atcgtttgaa tacgtggatt
     2401 gccgatgcgc caatcgcgct gcgtcagccg aagcgcctgg tcagggcacc gccaannnnn
     2461 nnnnnctggt agatgcgttg acccgttcga tgaacgtgcc gacggtgaat ctgggcatgg
     2521 cgttggggct gcctgcggtt acggagacct ggattaaact gggtgtgccg aaagatcaac
     2581 tgaatccagt tccagcaatg ttgttggggg cgttgaactt aacgccaatc gaagtggcgc
     2641 aggccttcca gactatcgcc agcggtggta atcgtgctcc gctttccgcg ctacgttcag
     2701 tcatcgcaga agacggcaag gtgttgtatc agagcttccc gcaggcggaa cgcgctgttc
     2761 cggcgcaggc ggcgtatctg acactatgga ccatgcagca ggttgtacaa cgcggtacgg
     2821 gacgtcagct tggggcaaaa tacccgaacc tgcatctggc agggaaaaca gggactacca
     2881 acaataacgt agatacctgg tttgcgggca ttgatgggag cacggtgacc atcacctggg
     2941 tcggccgtga taacaaccag ccgaccaaac tgtatggtgc cagcggggca atgtcgattt
     3001 atcagcgtta tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata
     3061 ttgcagatat gggcgt
//
LOCUS       DAWDRC010000485         3076 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12319_length_3076_cov_2.143330, whole genome shotgun sequence.
ACCESSION   DAWDRC010000485 DAWDRC010000000
VERSION     DAWDRC010000485.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12319_length_3076_cov_2.143330"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1106..1115
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2452..2461
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          877 a          806 c          694 g          679 t
ORIGIN      
        1 gcccggcccc tccataatag cgcgacgatg accgcagaac gcctgagcga agcaggccag
       61 tgcggtagca agaaaagcat attgcgtgag cgtaagcagg ctactttgcg gcaactgaaa
      121 agcagaaagt agcgtaggag gaaccacaac cgtgttgcaa aaaataaaga aaaaccactg
      181 aaatcctgat aacaggcttt cgcggcccac tgcaaaattg aacataaaca caccttaacc
      241 ggagaatgcc cgccacaagg acgggcattg aagggccaga ttactgctga tgtttaagga
      301 taaattgacc tttcggcgca acagggaagc cctgttcgat atcgtaaatc acatcaccac
      361 gtaagatggt tttcgtgata cgcgcgccaa tagtacggcc aacatacggg ctgactttgt
      421 ggcgatattc cagatcgtca ttggtaagaa catagctgct attcggctga atgaagacga
      481 agtcggcatc ttttcctggg gcgatacggc ctttttgctg cagaccgaaa atatctgctg
      541 cgttagtcgc cattaatttg ccgaacattg gcagagacat tccgcgtttc tgtaccgctt
      601 catcgaacat cacgtccatg cagctttgca gaccagcgat accgccccac gctttcatta
      661 tgttaccggc tttcatttcc ggcgggcatg gagagtggtc ggaaaccagg cagtctattt
      721 caccattaaa cagtttttcc cacatgcctt tctggttttc cagatcgcgg atcggcggtg
      781 aacacttcgc cagagtaccg atttcttcga actgatcggt atccaacaca aagtaatgcg
      841 ggcaggattc acaagtaaca tcctgacctt cctgacgtgc acgagtcact tcctcaacac
      901 cttccgggct gctgacgtgg caaacgtgca gacggcaacc agcgactttc gccaggtaca
      961 gtacgcggcg aatcgcttcc acttcggtaa ataccggacg cgaagccaca tagtcatggg
     1021 cagttacgcg accttcacgc ttcgcttctt cgcccagtgc gtcacagatc agcgcgtttt
     1081 cgcagtgcac cagcactggc tggccnnnnn nnnnncaccc agcttctgcg cgcctttgaa
     1141 gaattgccag tcattgacgt cacggaagtc gttgtcgata ccgcgatcgc cacaggtcgc
     1201 aacgaagcat ttgaaaccga caacacccac ttcatccaac tcatgcagac ggtcaatgtt
     1261 gtaagacacc aggccaccga gttgtgccgc atcgatagtc agctttcctt tagcggcatc
     1321 gaacttcagt tcaattgagg cgcggtcaac cgttgcaggc agctggttga gcggcatttc
     1381 gatcatggtg gtgataccac cttttgctgc tgcgcgagta ccggtttcat aaccttccca
     1441 gtggctgcga cccggttcag aaatatgggt gtgcgcatca accatgcccg gcgaaaccac
     1501 cagaccagac gcatccataa cgtcttttgc atcgcccaga tcctggccga tagcagcaat
     1561 ttttccgtct ttaacggcga tatctacaac gcgagcttcg ttttctaaaa taacggtgcc
     1621 gtttttaatg attaaatcaa aagacataac aaactccttg taaaatacag attggttttt
     1681 aactcaatgt tcaatatcgt gattaattaa cctatggttt tttgttctcc tgttttttct
     1741 gctgttgtac gtttctttaa taaggcgtag gccgcaaagg cgacgatgac gccgacaaac
     1801 catgaaacac gtgataacgg ttccataaag ggaataaact taccgccaag agataaaata
     1861 acggccacca gagttactga aaacgcggtg aggttaaaac cgttatcgta atatttataa
     1921 tcgccaggtg cggtgtacag ttcatcaaga ttaatttgcc cacgcatcac cacaaaataa
     1981 tgcgccatca tcacaccaat taccggacca agcattccgc cgataatatc gagaaacaga
     2041 taaatactgt cctgattttc cattaatttc cacgggcaga tcagcaggct gataatactg
     2101 gcaatcagta cgccgttttt ataggtcagt ttcgtcggtg caatggcggc aatctgataa
     2161 ccagccggaa taatattacc ggtggcgtta gtagagatag tcgtcatcag aataaccagt
     2221 accgcaaaga acgaggcgaa caggctgtcc caacgctgaa caatatccag cacgttccag
     2281 gtgtcagcgc cgtaatgaat actggctccg gcaataatac atactccggc gaccgcaaac
     2341 agaatatagg ccacaactaa acccagcgtt tgccccagcg cctgctcacg aaacgagtgg
     2401 gcgttttgcg taaagtcgga tgcgctcacc gccggtgccg cccagaccgc annnnnnnnn
     2461 naatcaccac cagaaacagg aagccactgt tttctgcttt ctgaataccg cccggaatgt
     2521 agtcaaagat tggaccgatc ccgaccagcg aaatcgccca aatcgccata ccgccgaaaa
     2581 cgatatagat gcacgggtta agaatggcag tgaatttatt taaaactttt ccgccgccaa
     2641 aaccaatacc aacgttaacc agccagaaga gtaaaaaagt aattaagccc ggtagagaaa
     2701 ggcctaacag cgtgaaatcg ccaccgagag tcaaaaatcc cggccagatt ttgccaatca
     2761 gaatcaagca ggccagtgac cccgcgtaac attgcaggcc aaaccacata atggcagcaa
     2821 taccgcctct taataatccg ggaaacagtg caccacgtac gccgtaagaa gcacgcagga
     2881 tcatggcaaa cggtacgccg tatttactgc ccgcagcacc gtttaatacc attaccgcgg
     2941 caatgaaaaa ggcactgagg ataattgcca gcataatact aaaggtagac aagccgagaa
     3001 taaaaaagcc gccaaccatc acataattgg gaacgttatg aaccgaaccc atccataagg
     3061 taaaatagtt aaatgt
//
LOCUS       DAWDRC010000486         3067 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12356_length_3067_cov_2.592297, whole genome shotgun sequence.
ACCESSION   DAWDRC010000486 DAWDRC010000000
VERSION     DAWDRC010000486.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12356_length_3067_cov_2.592297"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          687 a          854 c          780 g          746 t
ORIGIN      
        1 tggctggcgc gtctccggca gtttcgatgg cgcatgacac accccatagc cgcagtcgct
       61 gtaaaaaacg ggtcgcaaac tccgcctgca ttaacacttc gccgccagaa agcgtgacgc
      121 caccgccgga cgtgcgaaaa aaaatgtcat ctttcatcac ttcccgttcc agcgcgtcaa
      181 ggctgatatc gcggccaatc cgttcaaatg ccccggaggg gcattcatcc gcatcacgca
      241 aacatttcgc gcagtgcaga catttcgcct ctctgcgcac cgtctggatt ttgccggaga
      301 tcgactccgg attagcacac catgggcaaa gatgcggaca gcctttaaaa aagaccaccg
      361 tacgaatgcc ctcgccgtca ttcagtgaat aacgctgaat gttgaaaatg cgcgccacat
      421 catcgcggcg cgtttccaca acgttacagc tgatgcgctg tccggcggat gatgtcatcc
      481 tggatctcct tcgacagttc gacaaagaag gcgctgtatc cggcaacgcg caccaccagc
      541 ccggcataat cttgtgggcg ctgttgcgct tcccgcaacg tgtcggcgtt caccacgtta
      601 aactgaatat gctgcaactt aagctgggta aacgcccgta agaagtcggc cagtttgcgt
      661 aatcccgctt caccttccag ggtcgccgga gtgaatttca cgttcagcaa cgtaccgtta
      721 gacagcagcg tattatcgag cttactgact gatttcagta ccgccgttgg cccttgtgca
      781 tcctgaccca gcatgggtga caagccaccg tctgccagtt gttccccggc aaaacgaccg
      841 tctggcgtcg cgccaaccac cgatcccaac gggacgtgag cagaaacggt atacgatcct
      901 ggcgtgaagt agccgccgcg cgggttctgg tatttttcca cttctttgca gtagtggcgt
      961 aacagttcgg cgctgatgtt atccacctcg tcgatatcgt taccgtattt ctcgaagcgg
     1021 ttaattaagc gagcgcggac tttttcgcct tctggcgttg cgaagttggc ttttaatacc
     1081 gacagcaatt cgtcaaaact taaacgctgt tgatcaaaaa tcatcccttt caacgcatgg
     1141 agagaatcgc tcaggttggc gataccgatc ccctgtacgc cggagaagtt ataacgcgcg
     1201 ccgccatcgg taatatcgcg acctttttcc agacaatcgc tgataaaaga cgagagtagc
     1261 ggtacaggag cccagtcgcg atggccgata tcacaaatat tgctgccctc aaccatcagg
     1321 gtgatgtagt ggctgatctt ggcgcggatc tgttccagta aaccttcata agtcagcgca
     1381 gcgttgcctt cattttcatg caggcagatt tccatcactt tcagcaggtt aaacatcgcg
     1441 atgtcatgca agccgtaggt tctgccggga atagataatt ccacacagcc cactacggaa
     1501 tagtcgcgcg catcttccag cgaaacgcca cggttgagga acgctggcac taccacttca
     1561 tcgttaaaga tttgcggaat accggtgccg aggcgaatgg tttctgcggt tttcatcagg
     1621 aacggcgtgt cgatcaaggc gttagtgcgc acgccgaggt tcggttgcgg taattgcacg
     1681 ctttgataag cgtcaaggca aaggaacgaa agcacgttca ccgcgctacg tccgttctcg
     1741 gttaacccgc cgagcagtgc ggtatagccg gtagggaaac ctgcgaaata acgcgcgcta
     1801 ctggtggagc gcaacagcac gatgtcgttg catttcaccc ataaagattc gagcagttct
     1861 ttcaggaatg ccggatcttc gccctgggtt aatgatgtct gatagaacgg caacatatac
     1921 tggtcgaagc gccccaacga tagcgaactg gcgttggatt cgtattgcag aatgatgttc
     1981 atgtaccaga ataactggca cgcctgccag aacgtctgcg gcttatgttc ggcgttatgg
     2041 cgggagatct ccgcaatggt cagcagttct tcgcgacgct ggccatcagt gcagcttgcc
     2101 gccatcgttt ccgccagttc ggcgtaacgc aaaatatgtt tctgcgaggc ttccagcagt
     2161 aacagcgctg cctgataaaa gtgattctcc ggctgttgct gacaatgttg ctgcatctgt
     2221 gccaccagct cccccagccc gtgattcagc aggcgcgggt aatcaataat aatctgcccc
     2281 tggcctttat ccgtctggtt gatgctgaaa atctgcgtgc tggtcgcggc ttttacttca
     2341 tccgtcatct gcccgttgat gaaatctttc atcgaacgtt tttcccagta cgggaacaac
     2401 tcttcgcggt agatacgttt atcttcttcg ctgatagcaa agcggtcctg cggacgcgtc
     2461 gggaattgat ccagctcttt cagcagccag taagggtcca tttccggcga cataatcccg
     2521 gcgcgtggtt ttacggtgcg gttaccggca atcagttctt catcacgaat cgaaatttca
     2581 acgtgttcaa ggatatacgc tgttgctttc gcccgacgca ttatcaccgg ctccccttcg
     2641 gtttgccgat ggctggcggt ataaagcaac gcccgctcca gcgagatttc acgggtattg
     2701 gcaaacagtg cagttttgag gcgagagata cgattcgtca tgaggaactc ctgtgggaaa
     2761 gcgaacccgc ccttgcgggc gggataagaa cttaagcggt ttgtgccagg tgagcttcaa
     2821 ttttgctcat aatggcgtca gcacgcttta ccgcgtcgct gatgttgaca cgcacgatag
     2881 tcttgccagc aaaacgctct tcaaatttga tgccgatatc tttggtcaaa atcaccatat
     2941 cggcgcttgc cacgtcttcc gcagtcagtt cattttccag accaatcgac ccctgggttt
     3001 ctactttcac ttcccagcct ttcgctttcg cggcgctttc cagcgcctct gccgccatat
     3061 aagtatg
//
LOCUS       DAWDRC010000487         3063 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12373_length_3063_cov_2.036569, whole genome shotgun sequence.
ACCESSION   DAWDRC010000487 DAWDRC010000000
VERSION     DAWDRC010000487.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12373_length_3063_cov_2.036569"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1933..1942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          658 a          809 c          840 g          746 t
ORIGIN      
        1 accgcgcgag caatatatgg cggcgatgac ctttgtcacg ctgcccggtc aggtcgggcc
       61 gctgcttggc ccggcgctcg gcggtctgct ggtggagtac gcatcgtggc actggatctt
      121 tttgatcaac attccggtgg gaattattgg cgcgatcgcc acactgatgt taatgccgaa
      181 ctacaccatg cagacgcggc gctttgatct ctccggcttt ctgttgctgg cggttggcat
      241 ggcggtatta accctggcgc tggacggcag taaaggtaca ggtttatcgc cgctggcgat
      301 tgctggcctg gtcgcagttg gcgtggtagc actggtgctt tatctgctgc acgcccaaaa
      361 taacaaccgc gccctgttca gtctgaaact gttccgcact cgtacctttt cgctgggcct
      421 ggcggggagc tttgccggac gtattggcag tggcatgttg ccctttatga caccggtttt
      481 cctgcaaatt ggcctcggtt tctcgccgtt tcatgccggt agtgaatcgc tttggttatc
      541 gtcgggtact ggtggcgacc acgctgggcc tgtcgctggt caccctgttg tttatgacta
      601 ccgctctgct gggctggtac tacgttttgc cgttcgtcct gtttttacaa ggaatggtca
      661 actcgacacg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaacctgg
      721 cgagcagcgg caacagtctg ctgtcgatga ttatgcaatt gtcgatgagt attggcgtca
      781 ccatcgccgg gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca
      841 ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg
      901 cgtttgtctt tgccagagtg ccgaacgata cgcatcaaaa tgtggctatt tcacggcgaa
      961 aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat
     1021 tttcgccacc tgcattgtct tattgatcac gatgcactgg gcggtacgta tcagttttga
     1081 gcgcggcttt atcgattaca tcaagcatgg taatgaacaa cggctgcaaa tgctcggcga
     1141 tgcgcttggt gagcagtacg cccagcacgg gaactggcgc ttcctgcgta ataacgatcg
     1201 ctttgtattt cagatcctac gttcactgga gcatgataac aacgaagata agcccggccc
     1261 cggtatgcca ccacacggct ggcgcacgca attttgggtg gttgatcaaa ataataaagt
     1321 gctggttggc ccgcgagcac cagttccacc cgacggcaca cggcggccca ttatggtcaa
     1381 tggtgcggaa gttggcgcgg tgatcgcctc ccctgttgaa cgactgaccc gtaatactga
     1441 tatcaatttt gacagacaac agcggcaaac cagttggctg attgtggctt tatctacctt
     1501 attagcggcg ctggcgacat tcccactggc gcgcggtttg ctggctccgg taaaacgact
     1561 ggtggatggc acgcacaaac tggcagcggg tgatttcact actcgcgtag cccccaccag
     1621 cgaagatgag ttgggcagac tggcagagga tttcaatcaa ctcgccagca cactggagaa
     1681 aaaccagcag atgcgccgcg attttatggc cgatatttct catgaactgc gcacgccttt
     1741 agcagtactg cgcggcgaac tggaagccat tcaggatggt gtgcgtaaat tcacgccgga
     1801 gacgctggct tctttacagg cagaagtcgg tacactgaca aaactggttg acgatctcca
     1861 tcaattgtct atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat
     1921 cccactgctg gannnnnnnn nnatttggcg atcgcgatcg tttaatgcag ttgttcaata
     1981 acttactgga aaatagcctg cgctacactg acagcggcgg cagcctgaaa atctctgccg
     2041 agcagcacga caaaacggtg cgcctgacct ttgccgacag tgcgccgggt gtcagtgacg
     2101 atcagctaca aaaattgttt gaacgttttt atcgcaccga aggttcccgc aaccgcgcca
     2161 gcggtggttc cgggctgggg ctggcgattt gcctgaacat tgttgaagca cataacggcc
     2221 gcattattgc cgcccattcg ccttttggcg ggcttagcat tacagtagag ttaccgctgg
     2281 aacgggattt acagagagaa gtatgaccga gttaccaatc gacgaaaaca caccgcgtat
     2341 tttgatcgtg gaagatgaac cgaagctggg gcagttgctc attgattatc tgcgtgctgc
     2401 gagctatgcg ccaacgttga ttagccacgg cgatcaggta ctgccatatg tgcgccagac
     2461 accaccggat ctgatactgt tggatctgat gctccctggc accgatggcc tgacgctgtg
     2521 tcgggagatt cgtcgttttt ctgacattcc gatcgtgatg gtgacagcaa aaatcgaaga
     2581 gatcgaccgc ctgctcggac tggagattgg cgcagatgat tatatctgca agccgtacag
     2641 cccacgggaa gtggtagcgc gcgtcaaaac cattttgcgc cgttgcaaac cgcagcgcga
     2701 gttgcagcaa caggatgctg aaagcccgtt gattatcgac gaaggtcgtt ttcaggcttc
     2761 atggcgcgga aaaatgcttg acctgacgcc tgcggaattt cgcctgttga aaacgctctc
     2821 tcatgaacca ggaaaagtgt tctcccgcga gcaattgctc aatcatcttt atgacgacta
     2881 ccgcgtagta accgaccgca ccatcgatag ccacattaaa aacctgcgcc gcaagctgga
     2941 atctctcgac gccgaacagt catttatccg cgccgtttac ggcgtcggtt accgctggga
     3001 agccgacgcc tgccgcatcg tttagtttta gcgacatgac tttgttagcc ggagatggcg
     3061 ttc
//
LOCUS       DAWDRC010000488         3062 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12380_length_3062_cov_1.887928, whole genome shotgun sequence.
ACCESSION   DAWDRC010000488 DAWDRC010000000
VERSION     DAWDRC010000488.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12380_length_3062_cov_1.887928"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    631..640
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2476..2485
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          722 a          825 c          707 g          788 t
ORIGIN      
        1 tcgtcttctg ggtcagagta gcggcgcggc tctggtagcg ctgatgctaa atcagtttgg
       61 tgataatggt acgcacgtct cgctgatagc tgcggctatt ctggcagtga ttgctgcctg
      121 tgtcagtggt ttacgtatca ctcagccacg atccagggca taaaaaaagc gcgtcgatca
      181 ggacgcgctt tttagtattt acttcatatt acttcaggta ttcacccgta cgcagagctt
      241 caatacgttt atccagcggc gggtgagtca tgaacaactc actgagcgat ttcgacttcc
      301 cgttaatgca gaaagccatc atgctggttg cttcttgcgg ttcatagctg gttttcaggc
      361 gctgcaatgc agcaatcatt ttctcgcgac caaccagttt tgctgaaccc gcatcggcgt
      421 ggaattcacg atgacgcgag aaccacatgg tgataatgct cgccagaata ccaaacacca
      481 gttccagaac cgttgcaacc gcaaagtaga tcagcgggtt gccgttgctc tcttcacctt
      541 catcacggtt tccgcccata aaacccgcgg caagctgcgc cagaatacgg gaaataaaga
      601 taacgaaggt gttcaccacg ccctgaaaca nnnnnnnnnn gcgtcatggt gaccatatca
      661 ccattggcga tgtggctgat ttcgtgagca attaccgcct cggcttcatc cgggctcatg
      721 ttctgcagca aaccggtgct gacagcaacc agagaggcat cacggcgcgc accggttgca
      781 aaagcattga tgtccggcgc atggtagata gccacttgcg gcatagcgat ccccgcctga
      841 cgagcctggg ttgctacagt attgaccagc caacgttccc tttcgttacg cggtggctcg
      901 atcacttccc cgccaacaga tcgtaatgcc atccatttgg acatcagaag cgaaacgaag
      961 gaaccaccaa aaccgaacag caaggccatg atcatcagcc cctgaacgct gctcgactgt
     1021 atccctgtca ggctcagtac cagcccgaaa acgaccatta cggccaggtt cgttagcagg
     1081 aagagcgcga ttcgcatcat aattttcttt ttacctcagt ttaacaaaac gcaatatgcg
     1141 atacccacat cgtatgggtt acgcgactat tttcaagtct ggatagtgcg taagtcacca
     1201 gaaagacaca actttacatt ttgtagcatc tgatttacgg catcttgccg ctgttaaaaa
     1261 atcaggcaca atttcttgtg cctgattgat attacttgac gggagcgggt tgttccgcgg
     1321 gtctggcttt ttcaagcttc gccagatcga gtgcgatatt caccgtctca tccagataag
     1381 gatctggctc ctggtaatct ttcggtagat catccagttt cttcaactcc ggtttacctt
     1441 cgcgtttaaa gcgttcgttc aaacgcgcca gacgcgtcgc atcatcttca ttattctctt
     1501 tctcacgcac agcgtaattc agagaaacga tattgcgctt gtccttcata gcgttgaagc
     1561 gcgcgatatc cttcatgatg ttctggaact caggatcttt cgcgatacgc gcattatgtt
     1621 ccttcagcag ctccggttca aaggccgtta aatctcctga tttcacataa gtcgcggcat
     1681 caatgctatc ccacggcagc gcgttatctt cgaatttctc acccgtttcc gtttcttcat
     1741 tacccgtcgg catgatgatg tctggcgtta cgcctttacg ttgcgtactg ccgccgttaa
     1801 cgcgatagaa tttctggatc gtgtactgca cagaacccag cgctggccat tcaggacgta
     1861 acatctgatc gtaaatacgg ttcaatgaac ggtactgctg aacggtgcct ttaccaaatg
     1921 tcggttcacc cacaaccagg gcacgaccgt aatcctgcat tgccgcggca aagatttctg
     1981 aagccgaagc actgaagcgg tcaaccagca ccaccagcgg gcctttatag aagacctgtc
     2041 cgtcggtgtc gctgtcttca cgaaccttgc cgttgttatc gcggacctga acaatcgggc
     2101 cggatggaat gaacagaccg gagagcgata cggcttcagt caacgcccca ccaccattgc
     2161 tacgcaggtc gataatcaca ctgctgacat tctgtttttc cagtttctgc agttgcactt
     2221 tgacatcgtc tgtcaaaccc acatagaagc ccggaatatc cagcacgccg actttctctt
     2281 taccgacggt cttcaccgac attttaaccg cgcggtcttc gagacgaata cgttcacggg
     2341 tcaacgttac cgtacgggtc ttggtccctt taccagcagg taaaatttcc agacgaacct
     2401 tactgccctt aggcccttta attaaggcaa ccacatcata aagacgccag ccaatcacgt
     2461 aaaccatcgg cttgcnnnnn nnnnncatcg aattgataac ggtgtagtca tcatccattt
     2521 gcagcactgc gccaatacct tccagcgaca aactcatttc agtgttgaac tgttcggtat
     2581 tacgcgggga aagatagttg gtatgcgggt cgatttcacg cgcaaacgcc gtcattgcca
     2641 gcgagaaaac atcttcgctg ttggtttgcg ccagacgacg aatggcaaat ttgtagcggc
     2701 gagtcagggt ttcacgaatt tccttatccg tttttcctgt cagcttcagg cttaactcgt
     2761 cgaatttgac tttactgtcc cacagcgcgt tcaactcagc ctcgtttttc ggccagggcg
     2821 ctttgctgcg gtcaaggtta taagtgtcgt tgccggtgaa atccatcggc ttttccagta
     2881 ccgacaaagc gtactggtaa cgttcaaaac ggcgcttttg cgccagattg tagagatcgt
     2941 agaaaacgtc gagtttacct gaacgcagtt catcgcctaa ctcagttttc tttttcgcga
     3001 actgttcaac atcgcttgcc agcagcacat tgtggctgta atcgagcaga atcaggtagc
     3061 gg
//
LOCUS       DAWDRC010000489         3054 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12410_length_3054_cov_1.967322, whole genome shotgun sequence.
ACCESSION   DAWDRC010000489 DAWDRC010000000
VERSION     DAWDRC010000489.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12410_length_3054_cov_1.967322"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          789 a          722 c          765 g          778 t
ORIGIN      
        1 cactgcgatc ttgccgacaa gtggtaagaa actcctgcag tgtgaactct tcgtccttgg
       61 cagcttcata tcgctggcga tagtgatcga atatattcat ggtatgccgt cctttcgttt
      121 tttagcacag gttaagagcc gttcatatga atagttgagg ctcccggaag aggtaaactg
      181 aacgacgtct cttgctaaaa cacagcaact ctcatgccat tttattgacg atgagaacaa
      241 cgtgcccggt gatacacctt caaaattaag cgtagatggc aaatggaaaa cttgcctgca
      301 cggctttctt aatttcaatg gcatatcaac aactcatcca taaattttgc ataattaatg
      361 taaataatag ggtcgccggt aacgctaaat tcatttggct gtaagcgtgg tgtcatctgc
      421 gtcaggaaaa ttaaacagtt actataaaaa atgaaaacgt aaaaaggttg ggtttcgatg
      481 tattgacggg taaactttgt cgcccgctaa acatttgttt aaggaatgat taattgtgac
      541 caaactcaaa cttctggcac ttggagtgct tatcgcaacg tctgcaggcg tagcgcacgc
      601 tgaaggtaaa ttttccctgg gcgcaggcgt aggtgtcgtt gaacacccat ataaagatta
      661 cgataccgat gtttacccag taccggtaat caactatgaa ggcgataact tctggttccg
      721 tggcttaggt ggtggttact acctgtggaa tgacgcaacg gacaaacttt caattaccgc
      781 ttactggtcg ccgctttact tcaaagctaa agacagtggc gatcaccaaa tgcgtcacct
      841 ggatgaccgt aagagcacca tgatggctgg tctgtcttat gctcacttta cccagtacgg
      901 ttacctgcgt accaccctgg ctggcgatac cctggataac agcaacggca tcgtctggga
      961 tatggcctgg ttgtatcgtt acaccaacgg tggcctgacc gtgactccgg gtattggtgt
     1021 gcagtggaac agcgaaaacc agaacgaata ctattatggc gtatcgcgca aagagtccgc
     1081 tcgcagcggt ctgcatggct ataacccgaa cgacagctgg agcccttacc tggagctgag
     1141 cgccagctac aacttcctcg gcgactggag tgtttacggt accgcgcgct acacccgtct
     1201 gtctgatgaa gttactgaca gcccaatggt ggataaatcc tggactggcc tgatttctac
     1261 cgggatcacc tacaaattct gataatgcat ttttccaggg cattttgtgc ataaaaacag
     1321 ggcgtcatgc cctgttttgc attacggtgg tgcaatacct gggaggaagc aatgcaacaa
     1381 aaaatgattc aatttagtgg cgatgtctca ctgccagcca tagggcaggg aacatggtat
     1441 atgggcgaag atgccagtca gcgtaaaaca gaagttgctg cactacgcgc gggcattgaa
     1501 ctcggtttaa ccctcattga taccgccgaa atgtatgccg atggcggtgc cgaaaaagtg
     1561 gttggggaag cattaatcgg tctgcgtgat aacgtctttc tcgtctctaa agtctatccg
     1621 tggaatgctg gcgggcaaaa agcgataaat gcatgcgaag ccagtttacg ccgtctcaat
     1681 actgattatc tcgatctcta cttattacac tggtctggca gtttcgctct cgaagagact
     1741 gtcgcagcga tggaaaaatt gatcacccag ggaaaaatcc gccgctgggg cgtttctaac
     1801 cttgattatg ccgatatgca ggaactctgg cagctgccgg ggggaaatca gtgtgccact
     1861 aatcaggtgc tttaccatct cggttcacga gggattgagt acgatctact tccctggtgc
     1921 cagaaacagc agatgccggt gatggcttac agtccattag cccaggccgg gcggttgcgc
     1981 aatggactgt taaaaaatgc ggtagtcaac gaaattgcac atgctcacaa tatcagtgcg
     2041 gcacaagtat tgttggcgtg ggtaatcagc catcagggtg tgatggcgat tccaaaagcg
     2101 gcaactgtcg cacatgtcca acaaaatgcg gcagtgcttg aggttgaact ttcgtcagcg
     2161 gaattagtta tgctggataa agcgtatccg gcaccaaaag gaaaaactgc gctggatatg
     2221 gtgtgatgtt gatacgtgcc ggatgcatcg cacccggcac acaatattaa cgcaaattaa
     2281 cgtttcgcaa cgcgaatgga ttgcgccaga tgtgcaggtt tctctttggt gactttttgc
     2341 gtttctgaag cgtaagccgt ttctacacaa acaaaggtct tgtagccatc atccggcata
     2401 tcgcccatgc taactgaaag cgccggtcct gggttccagc ctacaacgtt cagatgatgc
     2461 tggtggccta cggcgataat acgattcagc gcttcgtcat taattacgct gcaatcttgc
     2521 ggattcagat acacgcgatc ggtacggtca gggaaggtct gaataccgtc ggtcagtaca
     2581 tcttctttcg cgtcatttac tttatcaatg aagcgatcgc ctagtccact gacgcttacc
     2641 ttagcgatat cacccacgtt aaagtaggta tgcagagcag aggtagtttc aaattcgccg
     2701 tgtgcttcaa gatcgatttc acaggtttta cccacgtgga aatgcgctaa cagcgtaaag
     2761 tcgtgcggcc agaatttttt cgtctcttcg ctttgtgtca attcaaaagt cagcgctacg
     2821 ccatcagcat cttcacgatg tgatttcagc gcccacggca ggttgcgggc aaaaccgtgc
     2881 gcaggcagac cttgttgtgc agccggacca aaccacggcc agcaaaccgg tatgccaccg
     2941 cgaatggcga cgccattttt gaacggtgtg ttgttactca accacagaac ttcttcttca
     3001 cccgcaggtt tccacgagag aaggtgtgcg ccctgtaatg caaaagaggc tttt
//
LOCUS       DAWDRC010000490         3053 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12416_length_3053_cov_2.553369, whole genome shotgun sequence.
ACCESSION   DAWDRC010000490 DAWDRC010000000
VERSION     DAWDRC010000490.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12416_length_3053_cov_2.553369"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          876 a          721 c          654 g          802 t
ORIGIN      
        1 aacgatcaat atcgttaata agtctgaact aaagaaaaat tttattatct acggtcctca
       61 gcttatttct tgtactaata acaacatgct aatccgctgt cttagacaag atacttcaat
      121 cgatatagaa tgccatgaca tactgctttc agctgaaaat gccgaagaac tgttggttca
      181 acgcaaaact gacctggcaa ttacgctgca accagtaatt agtcgctctg tcatctgcat
      241 gccgttacac actattcgca attcccttat atgtagcaac aaacatcctc gtattactga
      301 taccagtaac tatgaacaaa ttatcgccga agaattcacc ctgttgatat ctaaatcagc
      361 agggattgac gaaattcaaa tggacataga tgaacgtttt atgaaccgta aagtttcatt
      421 tcgcagttct tctttgatga ctataattaa tagtatttct gttactgact tattaggcat
      481 tgtgcccacc gaactgtacg atttacatcg tgactttttg aagttaaaag aaataaaact
      541 ggaacaacct cttcctgcgg ttaaacttta tatttcctat aacaaggctt cgttgaacaa
      601 ccttgtcttc tctcgattca tagatcgttt aaatgatagc ttctgattca ttaccactga
      661 caaataatat attacgcatc aattaattca ttgtgtgaat ataaaataat gtgctctatc
      721 ttgcttcgtt tatcctataa ttagcggatt catctcacga gtcaccccgt aacgttcaga
      781 gagcgttttt agatgtctat ttataaaatc ccgcttccac tgaatattct ggaagctgca
      841 aaagaacgta ttacctggac acttaatacc ctaccccgta tctgtgtttc tttttcgggt
      901 ggtaaagatt ccggattaat gctgcatctg acagctgaaa ttgcccgaca aatgggcaaa
      961 aaaatctgcg ttttgtttat cgactgggaa gcgcagttct catgcactat taactatgtt
     1021 cagtcattac gtgagtttta cgccgatgtc atcgaagagt tttactgggt tgcgctcccg
     1081 cttacgacgc aaaattccct ttctcaatac caacccgaat ggcagtgctg ggaacctgat
     1141 gtcgaatggg tgcgtcagcc cccgcaagat gcgataaccg atcctgactt tttctccttt
     1201 taccagcctg gtatgacctt cgaacaattt gtgcgtgagt ttgccgaatg gttttcgcaa
     1261 aagcgtccgg cggcgatgat gatcggcatc cgtgcagatg agtcctacaa ccgttttgtc
     1321 gccatcgcca gtttaaataa acaacgtttt gccgatgata agccctggac caccgccgca
     1381 ccaggcggtc atagctggta catttacccc atctacgact ggaaagtggc tgatatctgg
     1441 acctggtatg ctaaccatca acagctctgt aacccactgt ataatataat gtatcaggca
     1501 ggcgtccctc tgcgtcatat gcgaatttgc gaaccttttg gcccggagca acggcaagga
     1561 ttatggctct atcacgttat cgaaccggat cgctgggctg ctatgtgcgc acgagtcagc
     1621 ggggtaaaaa gtggcggcat ttacgccgga catgataatc atttttatgg tcatcggaaa
     1681 atcctcaagc cagaacattt agactggcaa gaatatgcat tattgctgct caatagcatg
     1741 ccggaaaaaa cagcggagca ttaccgcaat aaaatcgcta tttatttgca ctggtatcag
     1801 aaaaaaggca tcgaagttcc acaaacccag caaggggaca ttggcgcgaa agatatcccc
     1861 tcctggcggc gaatatgcaa agttttactc aataacgatt actggtgccg ggcattatca
     1921 tttagtccta cgaaagcgaa gaactatcag cgttataacg aacggataaa aggaatacgt
     1981 caggaatggg ggatactatg caacaacgat taacgcagga tttaacccaa tttctcgcca
     2041 gcttgccgga agatgatcgc atcaaagcga ttaatgaaat ccgcatggcg atccatcagg
     2101 ttagcccctt tcgtgaagag ccagtggatt gcgttctgtg ggttaaaaac agccaactta
     2161 tgcctaatga ttacaaccca aacaacgtgg caccacccga aaaaaaactg ctgaaaaaat
     2221 ccattgaaat agatggtttt acccaaccca ttgtggtcac gcatacggat aaaaatgccc
     2281 tggaaatcgt ggatggtttt caccgccatg aaattggtaa aggctcatca tcgttaaaat
     2341 tacgcttaaa aggttatttg ccggtgacct gtctggaagg tacgcgtaat caacgcatcg
     2401 ccgcgactat tcgtcataat cgcgctcgtg gacgtcacca aatcactgcc atgtcagaaa
     2461 tcgtccgaga actcagccag ttgggatggg acgataataa aattggcaaa gagctgggaa
     2521 tggacagcga cgaagtgttg cgcctgaagc aaattaacgg cctgcaagag ttgtttgccg
     2581 accgtcaatt ttcccgcgcc tggacggtta aataactaca gctggcgcag gcgttctgct
     2641 gctgccagca acgtcgattc cttcttggca aaacagagac gaatcagttt atgtgggaag
     2701 ggatcggcgc aaaacaccga cagcggaatc gccgctacgc cgtgctcccg cgtcagccac
     2761 tggcaaaact caacatcatc cagcgtagat acggcgctgt aatccaccag caaaaagtaa
     2821 gtaccttcgc acgctaagat ctccagccga ctctcactta aggcattcac cagaatatcg
     2881 cgcttctggc gataaaagtc cggtaacgca agatagtgct caggttctgc atgtagcata
     2941 tcggcaatcg ccagttgcgc cggggtattc accgaaaagg tcagatactg atgcactttg
     3001 cggatctcgg cgctgatggg cgcaggtgca acgcaatagc ccactttcca gcc
//
LOCUS       DAWDRC010000491         3049 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12446_length_3049_cov_1.887108, whole genome shotgun sequence.
ACCESSION   DAWDRC010000491 DAWDRC010000000
VERSION     DAWDRC010000491.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12446_length_3049_cov_1.887108"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    456..465
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    981..990
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1519..1528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2091..2100
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2670..2679
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          702 a          905 c          768 g          624 t
ORIGIN      
        1 gcgtacgaga tggcgcggat tataatgagc aacagggccg ttgactattg atgaaggtaa
       61 cattttttta aaataaaccc agtttaagca acaatcaggg acaaaacaga ctattgcatg
      121 acaaatttca ctgtcgctat ttgccccacg tcatccatca ccagcaattg ataatcgcct
      181 ttatccgtca aatgcagggt cacgttgcgc ccacgttcag ttaacggttc gccattcaga
      241 aaccaccagc gttcacctgc ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg
      301 cccggtaaac gtttaataat cgcgccatcg cgcacacctg ttagctgcag cgggagttgt
      361 gcatcgtgac cgtatggcgg gcaaattgtc gaggccggtg gcaagcgcac agcgcgacgt
      421 tctgatgcgg gcagccaggg ttccagcggt agcggnnnnn nnnnngccag acatttatca
      481 tttcttgtcg cgcctgaggg caatcagcgg caacacgttt gccgttatca tccagccaga
      541 tggggaaacg aatgccatta atgccttcct gctccggcag taatagagtt ggcggctggc
      601 tcccgtccag cagccaggtt gccaggcggc ggcgacagtt accgtcacct tccggcaaag
      661 actgtccgcc cggccagcag ataacgccac gacttacaga gtcaggacgt gggtcttccg
      721 gcaaattcac actgcgcgac agtaagatat tattaacctg attcaacagc ggtacggcac
      781 tggcaaagcc aaactgacca acaacgggcg tgccgtccgg tctgccagtc cagatcccaa
      841 tgacatagcg agcgttaacc ccaatcgccc aggcatcacg atagccatag ctggtgcccg
      901 ttttccatgc cagtggggcg acgcgcggca aggcaccatc cggcaagggt tgcgcttcat
      961 cagccataat ccggcgaatg nnnnnnnnnn cacgcagccc ccgacgacat taaaggccgt
     1021 tcaagcagcg gatcgtcagg ctgtaagcgc aatttacctg ccttgccgtg gcgagcaaac
     1081 gcggtatagg ccgccgccat atcttccagt tttgcaccag cgccgccgag aatgagcgaa
     1141 agattcggcg cagcaccgtt gggcagatac aacggcaatc caacattgcg taattttgcc
     1201 gcaaaccgtt tcggtccata ggcttccagc acctgcacag ctggtaaatt cagcgagcgc
     1261 accagcgcct cgctcatgct gatcggccca tgaaaaccgc tgtcaaagtt gccaggtcgg
     1321 taatcaccgg tacgccgggg gacgtcttgc aacagcgatg ccgggtggat caagccttca
     1381 tccagcgcca gaccgtaaac aaacggtttg agcaccgatc ctggcgatcg gatcgcattg
     1441 accatatcaa catgaccaaa gcgtgaatca tcgttgagat caaccgaacc cacccagccg
     1501 cgaacacgca tatcggtann nnnnnnnncg gtatgatcaa ccacgatcat cgccagtgag
     1561 ctgcgcggtg gtaatcgtcc tttccagttt tgcgccagtt cttccagacg tcgttgaaga
     1621 ccggcatcca gcgtagtaac gattttgtcg cttttgcttt taccgagcat catgcgcgaa
     1681 aacagcggtg ccagttgcgg catttgtcgg ggtgccagcc agatgggttc ttcccttgac
     1741 tcttttaccc gctcatggga ccacacacct tgcacggcca tccgttcgag cactttatta
     1801 cgcgcggctt cggcacgctc cggccaacga tccgggcgaa gacggctggg cgcttgcggc
     1861 aacaccgcca gcattgccgc ctcggaatag cttaaattcg caggcgattt cccgagataa
     1921 gcccaacttg ccgcaccaat cccctgcaac gtaccgccaa acggggcacg gttaagatac
     1981 aaggtcagaa tttcacgttt agacagatgc cattccagtt gcaacgcgcg ccagagctgg
     2041 cgaattttgc cgccaaacgt tttggggtga ggatcaagca ggcgcgccac nnnnnnnnnn
     2101 tgccgccgga aatgacccga cccgacgtga gatcttgcca ggctgcacgc gccaccgaga
     2161 acggattcac ccccggatgc ttccagaacc agcgatcttc ataattgatc agcgcttcaa
     2221 ggtaacgtgg agaaacatct tcgattgtta ctggataacg ccagatgccg tcagcatcgg
     2281 cgaaacgcca gagtggcgta ccatcctgcg ccaccaccac gcgtgcagga ttcacttctt
     2341 gcaatggcag aggccagagt ttatctgctg cccatgctgc cagaatgatg ataaaaggcg
     2401 cggctgccag cattatccag cagccgcgtt tggttaacag gcgaggcatt tacggtctga
     2461 ctatcaacag atcgtcagcc gcaccagtcg cccgccattg aggaacatac attgattcca
     2521 ccataggctg cggcacctga tacgttcccg gcgtcaccgc ccgcgccaga tacaccagcg
     2581 ttaccggttg atattcatca acggcaaccg ccgccacaaa gcgatcgtca cggaactcta
     2641 tgtgcttaat gctcgcctgc tgcatctggn nnnnnnnnng ttcagcaagt tctgcacttc
     2701 accaccactt tgctccaggc tggcgctgcc gttcgccaga ttctggtttt ccagttccag
     2761 ccccgcaggc aacagatcca cgactaacgc atccggtaca ctgttactgg ctttaacctg
     2821 caaccacacc agcaccagat cgccgctacg taacgagtcc agcgatttgc tcttaccgtc
     2881 agtacccaga atatgacgct caatttgcag cacattgctc gccggtaaag gtgcggattg
     2941 cggataaccg ctggcatcca cacgcagcca taacggcaga tcgccactgt tggtaacctg
     3001 caaggtggca agttgatcgc tattcagatt gctggtttgc gctttctcg
//
LOCUS       DAWDRC010000492         3041 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12468_length_3041_cov_2.036839, whole genome shotgun sequence.
ACCESSION   DAWDRC010000492 DAWDRC010000000
VERSION     DAWDRC010000492.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12468_length_3041_cov_2.036839"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    661..670
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1654..1663
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          680 a          853 c          778 g          710 t
ORIGIN      
        1 actgttacgc acgcccatcc gccagcgcat cgccagttgt ttttccagcg gcgggcaaaa
       61 cgcgccgaca ggcataaaga caggttgatg ttcgtcgagc ttcgcctgcg cctgcccgcc
      121 gtgcaacgtt ggcgtaacac ccatcagttc gaaaatggtt tcagtcagca cgcgggttgg
      181 atcttcgcta accccgtgaa ccaccacagg aaaaccgagt ttgtgcagca gaatcgccag
      241 caacggcgtc aggttggcct gtttacgcgc gccgttgtaa ctggggatga caatcggcat
      301 cggcttgcct gcaggcggcg tcagcttgat ggtgtgattt tgcatggctt cgtaaaagcc
      361 gagcatctct gcttcccctt cccctttgat acgtagcgca atcaatacgc cgcccaactc
      421 aaggtcaggg acttcaccat tgagcatatg ggcatacaga ccgcgcgcag tatcccggtc
      481 taaatcgcgc gcgtggtttt tcccgcgccc gatctcttta atgattttgc gatagtccat
      541 ttacgactcc ttacctgact cacatcatta acgccgccga cggcgtgggg atttcgtttt
      601 ttctttcttt ttaactatac cttcagggac ttccggttgc tctatcggaa ataccggtaa
      661 nnnnnnnnnn ccggtaatgc atccagtagt cgcttgccat aatttttggt cagcaagcgt
      721 ttgtcgtaga taaccacttc cccccagcaa ccgtggcttc gaatcagtcg cccaacctgc
      781 tgaatcaggt taaacgaagc gctcggcagg ctttgcacct caaacggata gcggttgagg
      841 cttttcagcc attcaccttc ggtgatcacc accgggctgt cgatgggcgg aaaagcgatc
      901 ttgtggatat gcacctggct gagcagatcg cctttcagat caagaccttc ggcaaaggac
      961 tgtaagccca ccagcacgct acgctcaccg ttggcaacgc gtttgcggtg cagttcaact
     1021 aagcgataac gtggctgatc gccctgcacc agcaacatca ggcgtaaatc cgtcacatag
     1081 tcgagaaagc gctgcatcgc acgcccgctg gcaaacagca ccaacatacc gagatgtttt
     1141 ttgctctcca cctgttcacg gaaaaaggcc gccatttcgg caatgtgctg ctcttcgttg
     1201 tcgatggaag gctcaacgcg catccgggga ataacaattt tgccctgttc acagtggtta
     1261 aaaggggaat ccagtgccac aaaacggtcg cccgctttct ctttcagccc actcatctcc
     1321 tgcaaacgcg aaaaactgtt cagcgaacgc aaggttgcgg aggtaacaat aatgtgcgga
     1381 atactgcgcc acagcagcct ttccagctga tcgctgacac gtattcccac gcagtgaaac
     1441 caaaggtgta gctgcccttc gcgctcttcc cgcgtcgccc atttggtcac cggcgcaccg
     1501 gaagattgcg ccagagacgc cagccgccag agtttgcttt gcgcctcgaa catccccaat
     1561 gcgcggttca tctgcaaaat caaccgatgc agacgtacaa tgtcatggct gccggttttc
     1621 tcacttaaat cgttaagaaa taactccgcc aggnnnnnnn nnncatctcg gtgagttttg
     1681 ccagccgctg gcagatctcc agcacttcat ctggcagttc gcccatcgca aaacggtgct
     1741 ctgcctcctg cccggcaggc atgtagagat tgagaatgtt gtttaacgag gcgataagct
     1801 catacaactc ttcacaatgc gcattcaaac gttcagggat cgccagcggc gggatggtct
     1861 tcgggcgaaa ctgctccatg caggtagcga ccagtttcgt gaacaggtcc agttggagcc
     1921 ggtaccacgg cgcggtgatt tcggcgctca tctccagcgc atcccgcgcc acatccggca
     1981 ggtgatgacc ttcgtccagc accagcagta aatttttcgg gtcaggcaat accgcttcgc
     2041 tttccatcgc cgccatcacc agcgcatggt ttgccaccac cacttccgct tcctgaattt
     2101 cccgacgagc gacaaaaaac gggcattcgc gatagtaata acagttgcgg ttgaggcagc
     2161 tggccttgtc ggtactcaaa cgacgccaga gatcatcatc tatagcgata tcagtatgat
     2221 cgcgcagacc atcccattta taagtgtcga gatcgccctt cagcttcgca caacgttttt
     2281 gctcttcctg attgttcggc gtcagttcat cgtcaagaaa cgccagcaga tcctgttgcg
     2341 tgggctcagt gctggcgagc gctgtcagat tacgcgggca aacgtagcgc ccacgcccaa
     2401 aagcggcagt gaatttaaga tcgggaatga tctttttcag cagcggtaaa tctttgctgt
     2461 agatctgagc ctgcaatgcc acgttggcgg tacttaccac cagcgttttt tgctcttcgc
     2521 gggcaatggc gatgccgggg atcaaatagg agagcgtttt cccaacgccg gtaggggctt
     2581 caatcgccag atgccgccct tcttctccgg ccagcgtttt ggcgacgtcc gcaatcatct
     2641 gccgctgcgg cgcacgggga ataaagtcgg ggatctgttc ctgaagcgcc ttataccagg
     2701 cggcaatttg ctctttaagc gcggcggtta atgccatgag aaaacctgaa atactgtata
     2761 aacagccaat attgtggcat ttttcggttt tcaggggtaa tggtttttct gtttcgggaa
     2821 agcggctcgc aataaccagc aggatgatgc gtaaagcgct tcgcgctaaa ttcgaacaac
     2881 atgcagaact gcgtacactc ttgcgggcaa ccgcgtccgc aaaactggtt gagcatacgc
     2941 aaaatgatgt ttactgggga gatggcggta atggtcaggg caagaatcgc ctggggtacc
     3001 ttttaatggc gttgcgcgga caattggctg cggagcagta a
//
LOCUS       DAWDRC010000493         3039 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12476_length_3039_cov_2.287198, whole genome shotgun sequence.
ACCESSION   DAWDRC010000493 DAWDRC010000000
VERSION     DAWDRC010000493.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12476_length_3039_cov_2.287198"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1725..1734
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2276..2285
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          671 a          758 c          920 g          670 t
ORIGIN      
        1 gctggcgctg gatgccatag agcgtttgca acgctggtgg gaggagagtg aggcactgga
       61 gccggagtct cgctttattg aaattccggt ggtttacggt ggcgcagccg gaccggattt
      121 ggcggtggtc gcggcgcatt gtggattgag cgaaaaacag gttgttgaat tgcactcctc
      181 cgtggaatac gtggtctggt ttttaggttt tcaaccgggc ttcccgtatc tcgggagttt
      241 gccggaacaa ctacacacgc cacggcgcgc tgaaccgcgc ttgcacgttc cggcaggttc
      301 tgtcgggatc ggcgggccgc agaccggtgt ttatccgctg gcaacgccgg gtggctggca
      361 gttgattggt catacctcac tcagcctgtt tgatccggag cgtgacgaac ccatcttatt
      421 acgtccggga gacagcgtgc gctttgtacc ccagaaggag ggagtatgct gaagattatt
      481 cgtgcgggca tgtataccac tgtgcaggat ggcggtcgtc acggttttcg ccagtcgggt
      541 atcagccact gcggcgcact ggatatgcct gcgctgcgca ttgctaacct gctggtgggt
      601 aatgacgcca acgcccccgc gctggagatc acgctcggtc agttaacggt tgagttcgaa
      661 actgatgggt ggtttgccct gacgggtgcc ggttgcgaag cgcggctgga tgataacgcc
      721 gtctggaccg gctggcgatt gccaatgaga gcaggccagc gtttaacgct taaacgcccg
      781 cagcacggga tgcgcagtta tctggcggtc gcgggtggta ttgatgttcc gccggtaatg
      841 ggatcatgca gcaccgatct caaagtgggg attggcgggc tggaaggccg tttactgagg
      901 gatggtgacc gactcccgat tggcaaagcg aagcgtgatt ttatggaagc gcagggcgtt
      961 aaacagctgc tgtggggcaa ccgcattcgc gccttgccgg ggccggaata tcatgagttc
     1021 gatcgcgcct cgcaggatgc attctggcgt tcgccctggc agcttagctc gcaaagtaac
     1081 cggatgggct atcgcttaca ggggcaaatt ttaaaacgca ccaccgatcg cgaactgtta
     1141 tctcacggtt tgttaccagg tgtggtgcag gtgccgcata acgggcagcc cattgtgttg
     1201 atgaacgatg cgcaaaccac cggtggttac ccgcgtattg cctgtatcat tgaggctgat
     1261 atgtaccatc tggcgcaaat tccgctcggt cagccgattc attttgtcca gtgttcactg
     1321 gaagaggcac tgaaagcacg gcaagatcaa caacgttatt tcgaacaatt agcatggcgg
     1381 ctgcacaatg aaaattgacc tgaacgccga tctgggcgaa ggctgcgcca gcgacgcaga
     1441 gctattaacg ctggtttcct ctgccaatat tgcctgtgga tttcatgcag gcgatgcgca
     1501 aaccatgcag gcttgcgtgc gtgaagcaat aaaaaacggt gtcgcgattg gcgcgcaccc
     1561 gagttttccc gacagggaaa attttggtcg cagcgccatg cagctgccgc cagaaaccgt
     1621 tttcgcccag acgctgtatc aaattggcgc gctggcagcg attacccgtg cgcaaggcgg
     1681 cgtaatgtgt catgtcaaac cgcacggcat gttatacaac caggnnnnnn nnnnaccagg
     1741 cggcgaaaga ggcacaactg gcagacgcta tcgccagagc ggtatacgct tgcgatccgg
     1801 cattgattct cgtcgggctg gcaggaagcg agctgattcg tgcaggcgaa cgatatggcc
     1861 tggtaacgcg cgaggaagtg tttgccgatc gtggttatca ggcagacggt tcgctggtgc
     1921 cgcgaagcca gccaggcgcg ttgattgaaa acgaagaaca ggcgctggcg caaacgttgg
     1981 agatggtgca atacggcaga gtcaaaagta tcaccggcga atgggcaatg gtcacggcgc
     2041 aaacggtctg cctacatggc gatggcgagc acgcactggc gttcgcccgc cgattacgag
     2101 ctacatttgc tgaaaagggg attgttgtcg cagcataacc ccgcttaata aagaatgaaa
     2161 aaaggatatc accatgcctg aaggcccgga gatccgccgt gcagcggata acctggaggc
     2221 ggcgatcaaa ggcaaaccac taactgatgt ctggtttgcc ttcccgcagt taaaannnnn
     2281 nnnnngtcaa cacgttaccc atgtggaaac gcgtggtaag gcgttgttaa ctcatttttc
     2341 caacgactta acgctctaca gccataatca gctttacggc gtctggcgcg tggttgatac
     2401 cggcgaagag ccgcagacca cgcgagtatt gcgggtaaaa ctgcaaacgg ctgacaaaac
     2461 cattctgctt tatagcgcct cggatattga gatgttgact ccggaacaac tgaccacgca
     2521 tccgttttta caacgcgttg gtcccgatgt gctggatccg aatctgacgc cagaggtggt
     2581 aaaggaacga ttattgtcgc cgcgctttcg taaccgtcag tttgctggat tactgctcga
     2641 tcaggcgttt ctggcaggac ttggcaatta tttgcgggtg gagatcctct ggcaggttgg
     2701 gttgaccgga aatcataaag cgaaagatct caatgcggcg caactggatg cactcgcaca
     2761 cgcgttactg gatattccgc gactttctta cgctacgcgg gggcaggtgg atgaaaacaa
     2821 atatcatggg gcgctgtttc gctttaaggt ttttcatcgt gatggcgaac cttgcgaacg
     2881 ctgtggcggc atcattgaga aaaccacgct gtcatcgcgc ccgttttact ggtgcccagg
     2941 ctgccagcac taggccgaac gcttcggaga ataggttgaa ataaaccgtg cgatggcagg
     3001 ccccgtcagc aaaatactga acaggcgcag ggtttgcat
//
LOCUS       DAWDRC010000494         3017 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12574_length_3017_cov_2.206280, whole genome shotgun sequence.
ACCESSION   DAWDRC010000494 DAWDRC010000000
VERSION     DAWDRC010000494.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12574_length_3017_cov_2.206280"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1179..1188
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          632 a          842 c          851 g          682 t
ORIGIN      
        1 tgccggtcgt gccggaggtg tagagaatgc aggaggtttc gttggattcc agccatgcca
       61 ccggcacccg cgcgccgatg tgctgatggc gcaacgacgc gaaatcgaca tcccgcccgc
      121 taacgcgcgc cattttcgcc agcccgcgat ccaccagtaa aacgtgacgc ggctgatgct
      181 gtgcctgact tatcgcatcg tcgagcaatt ttttatacgg aatgatttta ccgccgcgcg
      241 ccccggcatc agccgagaca atcagcaccg gtttagcgtc atcaattcgc gccgccacgc
      301 tgtgcgaggc aaaaccccca aacaccaccg agtgaatagc accaatgcgc gcgcaggcca
      361 gcagggtaat atgcgcttcg gcaatcatcg gcatatacac cagcacccga tcgccacgct
      421 gcacgcccag cgagcgcagc attgacgcca ccgcattcac ttcgtcatgc agttggcgga
      481 aggtaaaggt acgctcttcc tctgtttccg aagagacggc aatcaatgcc agcgcctctg
      541 gctgtttctc cagccagcga tcgatagcgt tgtgacacaa gttggttcgg ccttcacaaa
      601 accaacgggc aaacggcggg ttgctgtggt cgagcgtttg cgtaaagggc gtctgccagt
      661 caatacgccg ggcctgctcg gcccagaact gctccggttc gttaatcgaa cgctgataaa
      721 attcgctaaa agacataatg ctctcctgtt gaacttacgc cttaccgccg tttacttaaa
      781 tgacgtacag gtcgagatac tcattgaccg gcatctgttc caggcgagtt ctgtcgagag
      841 aaacctccag aatgcgctgc tgctggcgag tcgggaactg gcgcgcgaga ttgattttga
      901 atttatcgac cagcttcgga atgccatcct gacggcggcg agcatgacca attgggtact
      961 ccaccaccac ttcttcaaat cgcgtgccgt cggtgaactc aagggttatg gcattggcga
     1021 tggcgcgttt ttccgggtcg tggtagtcag cggtaaacac cggatcttca aagcaattga
     1081 tcttctcgcg cagggcgtcg atgcgtttat cttgcgcaac gttgtcctcg taatctgccg
     1141 ccgttaagcg cccgaacagc agcgggatcg ccaccatgnn nnnnnnnnga atgcagtggt
     1201 cgcggtcagc cgggttattg agcggccctt ttttgtcgat gatgcgaata caggcttcgt
     1261 gggtgcgaat ggtcactttt tcgatatccg ccgccgtttt gcctgctgcc tgcatctgtt
     1321 catagagcgt catcgctgct tcaactgccg tctgggagtg gaactccgcc gggaaggaga
     1381 ttttgaacag cacattttcc atcacgtaag aaccatacgg gcgctggaag cggaacgatt
     1441 cgcctttaaa ggagacgtcg tagaagcccc acacaggcgc agtcagggct gacggataac
     1501 ccatttcgcc ggttttcgcc atcagtgcca gacgtaccgc gcgggaagtg gcatcgcccg
     1561 ccgcccagga tttacgcgta ccggtgttcg gcgcatggcg ataggtgcgc agcgactgtc
     1621 cgtctaccca tgccagcgaa acggcgttga gaatttcctc gcgggtcagg ccgagcattt
     1681 cggcgaccac ggcggtggaa gccactttca ctaacagaac gtggtcgagg ccgacgcggt
     1741 taaaggagtt ttccagcgcg atgcagccct gaatttcatg ggctttgatc attgcggtca
     1801 gcacctgttt catggtcaac ggcgctttgc cgctggcgac cgcgttgcgc gaaagccagt
     1861 ctgccgttgc cagaatgccg ccgaggttgt cggaaggatg cccccactcc gccgccagcc
     1921 aggtatcgtt gaaatcgagc caacggatca tcgcgccaat gttaaatgcc gcctggacgg
     1981 ggtcgagctg aaactgagtt ccggggacgc gcacaccgtt gggtacgacg gtgccgggga
     2041 caattggccc cagcagtttt ttacaggccg gatattcgag agcttccaga ccacagccga
     2101 gcgtgtcgag caggcagtaa tgtgcggtgt cgtaggctac tctggagctg atttcgtagt
     2161 tcatcacgta atcgacgata tcaacgattt cacgatcaaa ttccgggcga atgttgttga
     2221 tttgagctga cattgggtac gtttccttat ttttattcgt agaggtttac tggcgcttat
     2281 ccagcgcgac aaactggcgg tcttccggtc caacgtaatt ggcggaagga cggataattt
     2341 tgttgtcctg acgttgttcg ataatgtgcg ccgcccagcc agtgacgcgg gcgataacaa
     2401 acagtggtgt gaatatctcg gtgggaacac ccatcatgtt gtaggaaaca gcggagaacc
     2461 agtcgagatt ggggaacatc tttttgctct cccacatcac tgtttccagg cgatcggcga
     2521 tgttgtacat cttcagcgag ccgccttcct gcgagagctg ctttgccacg cgtttgatca
     2581 cctggtggcg cgggtcggcg atggtataaa ccggatgccc aaaaccaatg accacttctt
     2641 tgttttccac ccgcttacgg atatcagctt cggcttcgtc cggcgtttcg tagcgttgct
     2701 ggatctccag cgacacttca ttcgccccgc cgtgtttcgg cccgcgcagt gcgccaatcg
     2761 cgccaataat ggcggaatac atatcagagc cagtgcccgc aatcacccgg ctggtaaagg
     2821 tggaagcgtt aaactcgtgt tcggcgtaca gcaccagcga gatatgcatc gccttttccc
     2881 agctttgcga cggcttttcg ccgtgcagca gatgcaggaa gtgaccgccg atagagtcgt
     2941 catcagtttc cggctggatg cgttcgccgt tgtggctgta gtgataccag tagagaagaa
     3001 tcgaactaag tgacgcc
//
LOCUS       DAWDRC010000495         3009 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12610_length_3009_cov_2.249154, whole genome shotgun sequence.
ACCESSION   DAWDRC010000495 DAWDRC010000000
VERSION     DAWDRC010000495.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12610_length_3009_cov_2.249154"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          657 a          797 c          765 g          790 t
ORIGIN      
        1 gtgaaacggc aggccaaatt ttagtagagt cgcacaatag ccccccaccc gttctgcccg
       61 ggttaatgag gaactttgcc ctcccagcca ggcgatacgc tgatgcccat tgcgaatgag
      121 atgctccgtc aacaactgtg cagcctgcat gttgtccggg cgaaccgtat caacgtcatc
      181 aagataactg gcacgggaag cgaaaatcac cgggatagct ttttcttctg ctatccgtcg
      241 caggtcatcg ctgcttcctg cagccccggc aatgaccaca ccatcgacac cctgattcag
      301 taatagtgaa aaccgctgtg ccagctgctc gccgtcttta ccgccgtgaa gcaaaaaaac
      361 catccgtccc tgcgcttcca gagcttccgt caatccggcc gtcaattcgg cgtaaaacgg
      421 cgcagataaa tcacggacga tcaaaccaat gacgccgctt tgcccgccgc gcagcgccga
      481 cgcctgacga ttgcgcacaa atcccagctc ttcaatggcg gcgttaacgc gttctcctgt
      541 ggcggtagag attcgccctt tgccacttag caccagcgaa acggtactta ccgacacgcc
      601 cgcagccagc gcaacatcat gaatggttat ttttttggcg gtagccatgc agatgaccta
      661 ctccctgatt atgtgacaga taaaacgttt taccttttat ttatcttata cccgctatta
      721 tcgttgcgta atgtgattta tgcctcacta aaatttgata aaacgtttta tcttctcgcg
      781 caatttactg aatccagatt gttctctacg aggagtcgtt ttatgacggc gaaaacagca
      841 ccgaaagtca cgctgtggga gttcttccag cagttaggca aaaccttcat gttacccgtg
      901 gcattattgt cgttctgcgg cattatgctc ggcattggta gttctcttag cagccatgat
      961 gtcatcaccc tgatcccggt cctgggcaac cccgtgttgc aggctatctt tacctggatg
     1021 agtaagattg gctcgtttgc ttttagtttc ctgccagtca tgttctgtat cgccatcccg
     1081 ctgggtctgg cacgcgaaaa caaaggcgta gcggcattcg ctggcttcgt cggttatgcg
     1141 gtaatgaacc tcgcggtaaa cttctggttg accaataaag gcattctgcc aaccacggac
     1201 gccgcggttc tgaaagccaa taacattcag agcattcttg ggatccagtc gatcgacacc
     1261 gggatcctcg gtgcggtgat cgccggtatt atcatctgga tgctgcatga gcgattccat
     1321 aatatccgcc tgccagatgc gctggcattc ttcggcggta cgcgcttcgt accaatcatc
     1381 tcctcgctgg tgatgggtct tgtcggcctg gtgattccat tagtctggcc gattttcgcc
     1441 atgggtatta gcggcttagg ccatatgatc aacagcgcgg gtgatttcgg accgatgctg
     1501 tttggtaccg gtgaacgtct gctgttgccg tttggtctgc atcacattct ggtggcatta
     1561 attcgcttta ccgacgcagg cggcacgcag gaagtctgcg gtcaaaccgt cagcggtgca
     1621 ctgaccatct tccaggcgca attgagttgc ccgaccactc acggtttttc tgaaagcgcc
     1681 acgcgtttcc tttcgcaagg taaaatgcct gcgtttctcg gcggtctgcc aggtgcagcg
     1741 ttagctatgt atcactgtgc gcgcccggaa aatcgccata aaattaaagg tctgctgatt
     1801 tctggcctga tcgcctgtgt cgttggcggc acgaccgaac cgctggaatt cctgttcctg
     1861 ttcgtagcgc cagttctgta tgtcatccac gcgctgttaa ccggcctcgg cttcactgtc
     1921 atgtctgtgc tcggcgtcac catcggtaat accgacggca atatcatcga ctttgtggtg
     1981 ttcggtattt tgcatggtct gtcaaccaag tggtacatgg tgccagtagt ggcggcaatc
     2041 tggtttgtcg tttactacat catcttccgt ttcgctatca cccgcttcaa tctcaaaacc
     2101 ccggggcgcg atagtgaagt tgccagttca atcgaaaaag ccgttgccgg tgcgccgggt
     2161 aaatcaggtt acaacgttcc gccaatcctc gaagccttag gcggtgccga caatattgtc
     2221 agtctcgata actgcattac gcgtctgcgt ttgtctgtaa aagatatgtc gcttgttaat
     2281 gtgcaggcac tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca acataacctg
     2341 caggttgtta tcgggccaca agttcagtca gtaaaagatg aaatggtcgg tctgatgcgt
     2401 actgtccagg cataaggata agatatgttc gatttttcaa aggtcgtgga tcgtcatggc
     2461 acatggtgta cgcagtggga ttatgtcgct gaccgtttcg gcactgctga cctgttaccg
     2521 ttcacgattt cagacatgga ttttgccact gccccctgca ttatcgaggc gctgaatcag
     2581 cgcctgatac acggcgtgtt tggctacagc cgctggaaaa acgatgagtt tctcgcggct
     2641 attgcccact ggttttccac ccagcattac accgccatcg atcctcagtc ggtggtgtat
     2701 ggcccttctg ttatctatat ggtttcagaa ctgattcgtc agtggtctga aacaggtgaa
     2761 ggcgtggtga tccacacacc cgcctatgac gcattttaca aggccattga aggtaaccag
     2821 cgcacagtaa tgcccgttgc tttagagaag caggctgacg gctggttttg caatatgggc
     2881 aagctggaag ccgtgctggc gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag
     2941 aatcctaccg ggaaagtgtg gacgtgcgat gagctggaga tcatggctga cctgtgcgag
     3001 cgtcatggt
//
LOCUS       DAWDRC010000496         3007 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12614_length_3007_cov_2.366192, whole genome shotgun sequence.
ACCESSION   DAWDRC010000496 DAWDRC010000000
VERSION     DAWDRC010000496.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12614_length_3007_cov_2.366192"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2749..2758
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          747 a          722 c          789 g          739 t
ORIGIN      
        1 tcaccatgct atttgatgga gtgctgagcg cactggttag agcgcgcctg tttatgcagg
       61 acaacaatca gccaggcaaa ggtgtctctt tgtcaaaagc gatcaacatc attgagaacg
      121 gactgcgggt gagtcttgat gaagagagca aagacgaatt aacccaaaac ttaattgctc
      181 tttatagcta tatggtcagg cgtttgctgc aagccaattt acgcaacgat gtctccgcag
      241 tcgaagaggt ggaagcatta atgcgtaata ttgccgatgc ctggaaagag tctttactct
      301 ccccttcttt gattcaggac ccagtctgat gaacaatgca ccgcatttgt atttcgcctg
      361 gcaacaactc gtcgaaaaaa gccagctcat gttacgcctg gcaacggaag aacaatggga
      421 cgaactcatc gccagcgaaa tggcgtatgt gaatgcggtg caggagatcg cacacctgac
      481 tgaagaggtt gccccgtcca ctacgatgca ggagcagctc cgcccgatgc tgcacctgat
      541 tctcgacaac gaaagcaagg taaagcagtt attacagatt cggatggatg aactggcgaa
      601 actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt gcctatggcg atcagggcgg
      661 ctttgtgctg gctccgcagg ataacctctt ttgaatctta atgagccgat ggctcgcgaa
      721 taatccgatt acggctacgc ttctaatatt ccccttgaat ggagtcgaag aatgcgtaat
      781 cccacgctgt tacaatgttt tcactggtat tacccggaag gcggtaagct ctggcctgaa
      841 ctggccgagc gcgcagacgg ttttaatgat attggtatca atatggtctg gttgccgccc
      901 gcctataaag gcgcatcggg cgggtattcg gtcggctacg actcctatga tttatttgat
      961 ttaggtgagt ttgatcagaa aggcagcatc cctactaaat atggtgataa agcacaactg
     1021 ctggccgcca ttgacgccct gaaacgtaat gacattgcgg tgctgctgga cgtggtagtc
     1081 aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgat
     1141 gaccgtacgc aaattgacga agaaatcatt gagtgtgaag gctgtacgcg ttacaccttc
     1201 cccgctcgtg ccgggcaata ctcgcagttt atctgggatt tcaaatgttt tagcggtatc
     1261 gaccatatcg aaaatcctga tgaagatggc atatttaaaa ttgttaacga ctacaccggc
     1321 gaaggctgga acgatcaggt tgatgatgaa ctcggtaatt tcgattatct gatgggtgag
     1381 aatatcgatt ttcgcaatca tgccgtgacg gaagagatta aatactgggc gcgctgggtg
     1441 atggaacaaa cgcaatgcga cggctttcgt cttgatgcgg tcaaacatat tccggcatgg
     1501 ttttataaag agtggatcga acacgtacag gaagttgcgc caaagccgtt gtttattgtg
     1561 gctgaatact ggtcgcatga agtcgataaa ctgcaaacgt atattgatca ggtggaaggc
     1621 aaaaccatgc tgtttgatgc gccgctgcag atgaaattcc atgaagcatc gcgcatgggt
     1681 cgcgactacg acatgacgca gatcttcact gggacgctgg tggaagccga tccttttcac
     1741 gccgtaaccc tcgttgccaa ccacgacacc caaccgttgc aagccctcga agcccccgtc
     1801 gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt tgcgggaaaa tggcgttcct
     1861 tcggtgttct atccggacct ctacggtgca cattacgaag atgtcggtgg tgacgggcaa
     1921 acctatccga tagatatgcc aataatcgaa cagcttgatg agttaattct cgcccgtcag
     1981 cgtttcggcc acggtgtaca gacgttattt ttcgaccatc cgaactgcat tgcctttagc
     2041 cgcagtggca ccgacgaata tcccggctgc gtggtggtca tgtcgaacgg agatgatggc
     2101 gaaaaaacca ttcatctggg agagaattac ggcaataaaa cctggcgtga ttttctgggg
     2161 aacctgcaag agagcgtagt gacagacgaa aacggcgaag cgaccttctt ttgcaacggc
     2221 ggcagcgtta gcgtgtgggt tatcgaagag gtgatttaaa tccatccccg gcggcaagcc
     2281 ggggagattt cattacggca gtggcgtcgg taacggcgct ttatccagcg cggcggcaca
     2341 ctctgccgtc gggcgatcca cacgctccat cgtcatacca tcgtactcaa tggtgttacc
     2401 gtcacgctca acctcgtaca gctcacgttt tacggtaacg ttagtgagat catcagacat
     2461 caatgtcaac ttacccggca cggcaatcac ccgctgccac tgacggcaat cgagggtatc
     2521 gccctcttta gtgacaatga ggctgcctat cgcttccgga ctgaccaacg cacgttgcgg
     2581 ccctttggtc tgccagtaac ccgtcagcca gtccggcgcg ggggttttca cgacattgtt
     2641 atagttttcg acttcggcac agcccgccag caacatgagc gcacctgcaa ttgcgagttt
     2701 tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg gcattaaann nnnnnnnntg
     2761 agttcagagg ctacattcat gtcatggcag caattcaaac acgcctggtt gattaaattc
     2821 tgggcgccca tccccgcggt catcgcggcg ggtattctct ccacttacta ttttggcatc
     2881 actggcacct tttgggctgt cacaggtgaa tttacccgtt ggggcggtca gctcctgcag
     2941 ttgttcggcg tccatgctga agagtggggt tattttaaaa ttatccatct ggaaggatcg
     3001 ccattaa
//
LOCUS       DAWDRC010000497         3001 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12638_length_3001_cov_2.738968, whole genome shotgun sequence.
ACCESSION   DAWDRC010000497 DAWDRC010000000
VERSION     DAWDRC010000497.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12638_length_3001_cov_2.738968"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          751 a          786 c          699 g          765 t
ORIGIN      
        1 aggtcgcaaa atcggcgaaa ttccatacct gctcaccgac gacagcgctg acgcgatcaa
       61 agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt
      121 acattgagtg caggccggct aacgtatcca caccgtattc ggtgatgata atcggctgat
      181 gcagtttctc ctgccaggcc agaagttctt tttccagaac cttctctgcc gtttccaaat
      241 ctccgctttg gacataccaa ccgtaataac ggttcaggca cagcacatca aagagatcgc
      301 tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac acaggtgatc ggacgcgtcg
      361 ggtcgagttt acgcgttgct tccgccagtg gcgcaaaata ttcccgtgca ccttgcggac
      421 gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg
      481 caatcagctc tttaatcgcc tgtaagtgcg cctgctgggt ttccccgttg actgcctctt
      541 cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa gagaggttaa
      601 agccgacagc tgcagtttca tcaatcacca cgattccatg ttcatctgcc cagtcgagca
      661 tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta
      721 gtgcgtgatc gtgcaccatc agcacgttat cgaatccttt gccgcgcaaa tccgcatctt
      781 catgacgacc aaagccagta aagtagaacg gtttgtggtt gatcaggaac tgttcgccct
      841 tcactgccac tgaccggata ccgacgcgca gcaggtagat atcacactct gtctggcttt
      901 tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca
      961 ctacttgcaa agtcccgctg gtgccttgtc cagttgcaac cacctgttga tccgcatcac
     1021 gcagttcaac gctgacatca ccatttgccc ccacctgcca gtcaacagat gcgtggttac
     1081 agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtattc ggcgtggtgt
     1141 agagcattac gctgcgatgg atcccggcgt agttaaagaa atcatgaaag taagactgct
     1201 ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt
     1261 cgttgttcac acaaacagtg atacgtacac ttttcccggc aataacatac ggagtgacat
     1321 cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta tttacccaca
     1381 ctttgccgta atgagtgacc gcatcgaaac gcagtacaat acgctggccc ccccaacctt
     1441 tcggtataaa gacttcgcgc tgataccaga cgttgcccac ataattacga atatctgcat
     1501 cggcgaactg atcgttaaaa ctgcccggca cagcaattgc ccggctttct tgtaacgcgc
     1561 tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc
     1621 cgtcgagttt tttgatttca cgggttgggg tttctacagg acgtaacata agggactcct
     1681 cattaagata aaaatactgg tcaaccttta atctgattag ttaaccaatt acgtgttgca
     1741 cacagttatc tgtgagaaac ccatggaaga gaaatggaaa taaaaagaac tgacaccgcg
     1801 atcaaaaaac caaaaactgc aatacccaca gcgtgatatt gatcgcatta atctttaaaa
     1861 cagcgttgac agaagccaca aaataagcaa tcgaacgttc gttcgaacat attgggagcg
     1921 ataaactctc atgatatatt gctacgatga atatttaggt ggaattctcc gatccctggg
     1981 aggatgattt ttgcacagag aaattacgat atgaaacgcg ggctcagttg tcaccagccc
     2041 gcggaaagag agaggatcag gatgcggtta atataccgcc aatcatcgcg cgtaatccct
     2101 gagcaaacga cgcttcacgc gccagatctt ccgcttcgat atcggccagt acaccgtagg
     2161 tcagatccag cagtaaacgt gaagtcattg ccgtgttgat atctgggctt atctcgcctt
     2221 tttgctgggc atcacgcatc cgctgagcaa caaattccgt gatcgtcata tgcttatttt
     2281 taagcatggc ggcgacctgt gggttacgca tcccttccgc cataatttcg accaccagcg
     2341 cccgttgccc aaaggcctca tgggtgagag agacaataga ctcgaccata tagtcaacga
     2401 aatgaatccc ttcaatcggt tcccggaaac gggccagcgc cctctcctgg tcctgtaaga
     2461 taatcgcctg aatcaaggct tctttggaga tgaaatggtg atagagcgtc ccgggactaa
     2521 tggcgcagga tttacagatg gctttcatcg aggcactgtg aaatccattt tctgaaaaaa
     2581 tctctctggc agcattgagg atccgggtcc gtgtcggttg tgcttcagtc tgcatgttat
     2641 ccatcattaa accattatag ttagcatgaa taactagttt accgcgttgt caggtgagcg
     2701 cctatcattg ctgttgaata gtgcgaaggc acactcaatt cgtaagcttt ttatccagat
     2761 attggttaac caatagccgt gatttggctg ccagttgttt agcagagacg ttgatgagtt
     2821 gcacacgcta tcccggtaaa caacacatac ggctgtttac cggttcgtta gtgtattaat
     2881 tgagttcctg taccccacca ccggagacgg tgagaatttg tccgcttacc cagctggctg
     2941 caggcgagca aaggaacagc gccgcgttag caatatcttg cggttggccc agacgtctga
     3001 t
//
LOCUS       DAWDRC010000498         2989 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12683_length_2989_cov_2.211656, whole genome shotgun sequence.
ACCESSION   DAWDRC010000498 DAWDRC010000000
VERSION     DAWDRC010000498.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12683_length_2989_cov_2.211656"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1392..1401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1685..1757
                     /estimated_length=73
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          758 a          727 c          689 g          732 t
ORIGIN      
        1 tttcgcgctg ccatgaacga atggattaat ccccatcgcc acgccttccg ggtttttatg
       61 caccagcagc tcgcagccgt aggctttcgc cgtacgatcg cggaactcat acatctcgcg
      121 gaatttccag ccggtatcga catgcagcaa cgggaaaggc agcgtacctg gataaaacgc
      181 cttgcgcgcc agatgcagca tgacgctgga atctttaccg atagagtaga gcatcaccgg
      241 atttgagaat tctgccgcca cctcgcgaat aatgtggatg ctttccgcct ccagttgccg
      301 caagtgggta agtcgttttt gatccataac cgttcctttg caataccgct attttcttgc
      361 catcagatgt ttcgactata gggagcgtaa gagaacgaat gaaattacca attagaatga
      421 gtagttcctt aacggaataa cgatttggca aagctaatat caaaaagtgc ttaaggcacc
      481 ggatttcggg cgtttaggaa gatttgaaat tgttttagcg cagcggcagt ttcatactat
      541 ggcggtaaaa aaatttgcat ggtatttaag gactcactat gttttccgca ttgcgccacc
      601 gtaccgctgc cctggcgctc ggcgtatgct ttattctccc cgtacacgcc tcgtcaccta
      661 aacctggcga ttttgctaat acccaggcac gacatattgc tactttcttt ccgggacgca
      721 tgaccggaac tcctgcagaa atgttatctg ccgattatat tcgccaacag tttcagcaaa
      781 tgggttatcg cagtgatatt cggacattta atagtcggta tatttatacc gcccgcgata
      841 atcgcaagag ctggcataac gtgacgggaa gtacggtgat tgccgctcat gaaggcaaaa
      901 cgccgcagca gatcatcatt atgacgcatc tggatactta cgccccgctg agcgatgctg
      961 acgccgatgc caatctcggc gggctaacgt tacaaggaat ggatgataac gccgcaggtt
     1021 taggtgtcat gctggaactg gctgaacgcc tgaaaaatac gcctaccgag tatggtattc
     1081 gatttgtggc gaccagcggc gaagaggaag gaaaattagg cgctgagaat ttactcaagc
     1141 ggatgagtga caccgaaaag aaaaatacgc tgctggtgat taatctcgat aacttaattg
     1201 ttggcgataa gctgtatttc aacagtggcg taaaaacacc tgaagcagta aggaaattaa
     1261 cgcgcgaccg ggcgctggca attgcccgca gccatggaat tgccgccacc acaaatccag
     1321 gtttgaataa aaactatccg aaaggcaccg ggtgttgtaa tgacgcagaa atattcgaca
     1381 aagccggtat tnnnnnnnnn ntggaagcga ctaactggaa tcttggcaat aaggatggtt
     1441 atcagcaacg cgcaaaaaca gccgcattcc ctgcaggaaa tagctggcat gacgtaagac
     1501 tggataatca acagcatatt gataaggcac ttcctggaag aatagaacgc cgctgccgtg
     1561 atgttatgcg gataatgcta ccgttggtga aggagctggc gaaggcatct taatgggttt
     1621 gaaatgatta ctcccttgcc tgcaggaaat caatgccacc agaaacagag tataaaaaag
     1681 ggtgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnntct tcgattcgta agcgagaact gccttaaact ccataccacg
     1801 ttctctgata ctcaactcac acaacgagtc acctaccatc aacgtgaatc ccagtactgt
     1861 acaacacagt acgatgattg ccacaagggc atattttgtc agcatgtctt gcctccctgc
     1921 aaagaagaga ctaacatccg aattgctacg ttcgaacgcg agcctcaggt tgatagaaat
     1981 atcgcctggg gcttttgtcc atctggaacc tcgcgaatgc ttaacgccag acagcctcaa
     2041 gcacccaatg ccattctata cctgttaatt ctccctgcga cgttctcgtt taaatgccgg
     2101 aatgctgctc gcaaaataaa aacgccccat catttctgac agaggcgttt aatttatccg
     2161 gcaatattta cccttcgtgt aatccacatt ccctttttaa gccaaagaaa cgcgtttctt
     2221 cttccgccat gccgggttcc catttacggg ttgtatgggt atcgcccacc gataaatatc
     2281 cttcatccca taatgggtga tatttcaggc catgtttttg caggtactga taaatagttc
     2341 ggttatccca gtcgataatc ggcagcactt taaatacgcc acgctgaatt gccagcaccg
     2401 gtaaattggc gcgactgccg gattgttcac ggcgcaggcc agcaaaccag gtttgcgcat
     2461 tcagttcttt cagcgcccgg ttcatcggtt cgactttgtt gatgtcattg tacttttcaa
     2521 tgccttcaac gccctgctcc cacagtttgc cgtagcgtgc ttcctgccag gcggcgcttt
     2581 cggtagcacg gtacactttc aggttgagct tgagtttgtc cgttaactcg tcaataaagc
     2641 ggtaggtttc cgggaacaga tagccggtat cggtgaggat cactggaata tccgggcgaa
     2701 tctgattcac cagatgcaga ctcaccgccg cctgaatgcc aaagctggaa gaaagtacat
     2761 attcaccggg cagattatcc agcgcccagg ctacgcggcc ttcagcgtcc agtttttcca
     2821 gttgggcgtt agtttccgcc agcgccagaa tccggtctac cttcggcagt tcgttcaggg
     2881 cgtttagatc gagtttggac ataagttcct cactgtttgc cttgcctgat gcaacgctca
     2941 cacatcttat caggcctaca agaccgggct gatggttaat cccacaaat
//
LOCUS       DAWDRC010000499         2987 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12695_length_2987_cov_2.569236, whole genome shotgun sequence.
ACCESSION   DAWDRC010000499 DAWDRC010000000
VERSION     DAWDRC010000499.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12695_length_2987_cov_2.569236"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    238..247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          812 a          789 c          719 g          657 t
ORIGIN      
        1 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat ttagcgtgct
       61 gatatggctc agttggtaga gcgcaccctt ggtaagggtg aggtccccag ttcgactctg
      121 ggtatcagca ccacttttta ggttaaagtt cggcagatta gaaaagaatt tgtctggcgg
      181 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagnnn
      241 nnnnnnnggg tctccccatg cgagagtagg gaactgccag acatcaaatc aagcaaaagg
      301 ccatccgaaa ggatggcctt tttgcttttc gaactaacat tcaattaatg gattacctgc
      361 gataaaaatg ccctcgtacg ctctgattta ggattcgcaa aaaagtcatc aggtgcagct
      421 tgctccacta tttccccacg atccataaaa atcacccggt cagcgacggt tcgtgcaaac
      481 cccatctcat gtgttacaca caacattgtc atacccgact gcgccagccc aatcatcgta
      541 tccagcacct ctttcaccat ctcaggatcg agcgccgacg ttggctcatc aaacaacata
      601 attttcggct tcatacacag cgaacgtgct atggcaacac gttgctgctg accaccagaa
      661 atctgtccgg gaaacttctg ggcatgttcg gcaattctca cccgctcaag gtaatgcatc
      721 gccagagctt cagcctcttt ctttggcatc ttgcgtaccc aaatcggtgc cagggtacag
      781 ttctgtaaca cggtcagatg agggaagaga ttgaaatgct gaaagaccat tcccacttcc
      841 tgcctgacgc gctcaatatt gcggatatct tcattaagtt cgatgccatc taccacgatc
      901 cgtccctgtt gatgttcttc cagatgatta atacaacgaa tggttgtcga tttaccggaa
      961 cctgaagggc cacatagaac gatccgttcg cccggttgca cggttaaatt tatatttttc
     1021 aaaacatgga attgtccata ccatttattg acgttttcca gcgtaatcat cgcgttagca
     1081 ggttgcagta aaatttggct catagtgtcc tcaatgcggt gtacgcccgg tgttaaaacg
     1141 tttttccaga tgttggctat aacgtgacat gctgaaacag aagatccagt agatcagcgc
     1201 ggcgaaaaca tacccttccg tcgacatacc caaccaggcg ggatcaacgg ttgcctgctg
     1261 aacgctactg aaaagatcga acaacccgat gatgatcacc aggctggtat ctttgaagag
     1321 tgcgatgatg gtatttacca gcccaggaat taccagcttc aacgcctgtg gcagaataac
     1381 aagcccctga gttttccagt aacccaatgc cagcgactct gccgcttcat attgcccttt
     1441 aggcagcgcc tgtaatccgc ctcgcacgac ttccgcaaca tatgctgact gaaacaggat
     1501 cacgccaacc agcgcgcgga ttagtttgtc gatactggtg ccttctgcca taaacaacgg
     1561 cagcatgacc gaagacataa acaatacggt aatcagcggt acgccgcgcc agaactcgat
     1621 aaaaatgacc gataagatac gcacaatcgg catatgggag cggcgaccta acgccagtaa
     1681 gatcccccac ggtagcgccc ccgcaatccc aactgatgca ataattaaag ttagcgtcaa
     1741 cccgccccat tgccgggttt caacccgatc aagaccaaga aacccgccat acatcagcca
     1801 ccagacaacc agtgggtaaa tcaccgccca gaccgcaata tagcgaccgc gatgcggaag
     1861 tattttccag aacattggtg cgatagacac aaggccaatc agtaatgcca ggttaattcg
     1921 ccagcgttgg tcgtgtgggt aaagtccata tataaactga ccaaatcgtt cgtggatgaa
     1981 gacccaacag gcaccggctt ttgtgcagtc ggcacgcgtt gagccaaccc agttagcctg
     2041 caaaaatgcc cagttcagca atggtgggat cagttcccac atcaaccata tgcagccaat
     2101 agtcagcagg ctattgctcc agctggagaa cagatttttt cgcacccaca ccatcgcgcg
     2161 gcttgagtta tggctcgccg ggcgcggggg atgagacagc aatacttttg tcatcatgac
     2221 tccttagcgt tcaacgatcg cgatgcggcg gttatagata ttcatcagca gcgagatagt
     2281 caggctgata atcagataga ccgacatggt catggctatc gtctcgatgg cttgccccgt
     2341 ttggttcagc acggtgccag caaacagcga aaccatatcg ggatagccaa tagcggcggc
     2401 aagagaggag ttcttgacga tgttgagata ctggctggtt aacggtggaa taatcacccg
     2461 caatgcctgg ggaataatga cctggcgtag cgtgaccggg ttgggtaatc ccagtgaccg
     2521 agctgcttca tgttgaccat aaggcactgc ctggatccct gcgcggataa tttcggcgat
     2581 aaatgcagaa gtataaaccg aaagcgccag cgttaaggct gccagttcag gaattaaaac
     2641 catcccgccg cggaaattaa agcctcgaag ggctgggaca tcccagtgta gtgctgcgcc
     2701 aaaaagccat tgcgccagta aaggcaaacc aatgatcaac actgcggcaa tcggccaggt
     2761 gcggcgcagt tgtccggtct ttatctggtg tgttttatta aagcggaata gcccaacaga
     2821 aaggactata gctataacaa cagccaggat aaacgcaaga aacccatctc ccagctgcgg
     2881 cgacggaata taaagcccac gattgctcag aaacaccaaa tccagtgcgc tcactgcctg
     2941 gcgtggcccg ggcaaattgc gcaacacggc aaagtaccag aagaaga
//
LOCUS       DAWDRC010000500         2980 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12734_length_2980_cov_2.271111, whole genome shotgun sequence.
ACCESSION   DAWDRC010000500 DAWDRC010000000
VERSION     DAWDRC010000500.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12734_length_2980_cov_2.271111"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          728 a          794 c          714 g          744 t
ORIGIN      
        1 aatttaccga ccgttgccag aaaataacgc ccgcataaac gtccaagttt atagtcttca
       61 cgcagcgccg gaatccgact gccgagcgcg atactatgca atggcttcgg cggataaatt
      121 tcgaatatcc gcagctggcc cggtggtttc tcaataaatt gctgaatctc acgatagctt
      181 gtttcatgat gctgcaccag attgaccagc ggctgcaggc tactgtcacc cagccagcgt
      241 tccatgcgtt tgaaccactg cggcgtgtag tacatttgtg acggcacagt gcggatgacg
      301 accaacgttt ttgctccctg ccttgccgcc tctttaaccg ggatcgcatc actgatccca
      361 ccatccaggt agttaatacc ttccagcgac actccgctac gataaaagcc aggtatcgca
      421 ctggaggcgc gaatcacatc cagccagttt tgtttagttg gcaaaaagta attcggcgca
      481 tagtcatcct gacgacaggc gcacatataa aacgatttgc cgctgtcaaa taaccgcgct
      541 gcggtatcca tttgcaacgg catctggctt gcggtggcct ccaccagcca atcgagatcg
      601 ataagatttc ctccacggac aaagcgcaat ggatcgaaaa attcgcgttt tgtggtgtaa
      661 cgcatgatga ctttgcgcgc gtaaccgggc tgattgcaga tatacgccga gaggttctgc
      721 gccccggcag atgtaccgag ataaagatcg aaaggattaa actgcgcgcg cataaactca
      781 tccagcacgc cagccgtgaa aattccacgt tgtccgccgc cttcgcacac cagagcaatc
      841 cgtccaggct ggaacggcct tagcgacaac ggcgcaatat taccaagcgt tacaggtatt
      901 cgctgcccca cctctgcctt cctgtttttg ttgtgaaaga atgacaaagt aacgcaattt
      961 attattcgct aaaaccgcaa aagccagtcc gctggactgg ctttggataa aattttctta
     1021 atggtctgaa atctagggtc gttttcggcc catgaacaaa ctcaccagga acagaataat
     1081 cccgacgaca aagacaattt tagctgcgcc tgcagcagta ccggccagac caccaaaccc
     1141 aagtgcggcg gcgattaacg cgataaccag aaatatgatg ccccaacgaa acataagcgt
     1201 ctcctttacc atagttaatg tcaccgcttg atatgagcgt gaaaatcact caatgcgata
     1261 tttttagtgt ggtgcacatc gcgcctcccg acaaagttcg ggaggtcgaa ttacgacgaa
     1321 ttacttagtt ttcagatcat ttttaacgct tttcacacca tctaccgctt tggcgatact
     1381 ttcagcacgg tcgctctgtg cctgagaatc tacggtaccg gagagctgga ccacgccatc
     1441 ggtggtttca actttcacat gacgggaagg gacgatatcg tccgccagca gtttggcttt
     1501 gatttcactg gtagtggcgg tgtcacccgc gtagcctttc acagagcctt ctttagcgtc
     1561 gcgaacgtgc agtttgtcgc tgacagaggt cacgccctca acacctttcg ccactttcac
     1621 cgcctcttcg gcctgagcct ggctttcaac gaaaccgctc agggtcacga ctttttgatc
     1681 ggttttgaca gagatatcgg tgctcttgat gttgtcatgg tccaccaggg ctgccttcac
     1741 tttcgcggtg atggcactgt catccatgaa attaccgact ttattcatag agctatcgac
     1801 tttttgccct gcgctttcat tggtagtctg cgcgttgttt tctgcgtagg cagagccggt
     1861 cgcgacggca gaggtcaaca ttacagccag cagagttttc gaaatcttca gtcttgtcat
     1921 agtcatcgat ttattcctgt atgtttgctc gtaatttgag cctggcaaca cgaggttgca
     1981 ttgctgaata gggagagact tcaccctcta cagaagtcaa tggtcgccat cacaaaagcg
     2041 atgggtgatg aataacgacc attacagcct ctgaatcagt tattaatatc ggtagaatga
     2101 caatcgacgg ctttagatac tgatatctac gcattgaacg gtatttaacg ccgtcataaa
     2161 tgtcattact ttgttaaata tagatcacaa ttttgaaacc gctcgggata tcagctaaaa
     2221 cataagcaaa agtgaatgtt ttaagaacat tccgtaagcg gctaataagg aagggaaatt
     2281 gacagggcgc agcggctacc gcgccctgga ggctagaatt aatgctcgcg ggtctggtgg
     2341 aactgaacgt ccggataacg ttcctgcgcc aggcgcagat tgaccatgct ggtagcgatg
     2401 taagcgaggt tatcgccgcc atcaagcgcc agttggcttt cgttcttacg cttgaactct
     2461 tcgaatttct tcgcgtccgc acattctacc cagcgggcgg tggcaacgtt gactgactca
     2521 tacactgctt caacgttgta ttcgctcttc aggcgcgcta ccaccacatc aaactgcagc
     2581 acaccaaccg cgccaacgat cagatcgttg ttggagatcg gacggaacac ctgcaccgcc
     2641 ccctcttcgg aaagctgtac cagccctttg agcagctgtt tttgcttcag cggatctttc
     2701 aggcggatac gacggaacag ttctggcgcg aagttcggaa taccggtgaa cttcatcatc
     2761 tcaccctggg taaaggtatc gccgatctga atggtgccgt gattgtgcag gccgaggata
     2821 tcgcccggat acgcttcttc aacgtgcgaa cggtcgcctg ccataaaggt cagcgcgtcg
     2881 gagatcacta catctttcgc ggtgcgcacc tggcgcagct tcatgccttt ttcatattta
     2941 ccggatacca cgcgcataaa cgccacgcgg tcgcgatgtt
//
LOCUS       DAWDRC010000501         2972 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12765_length_2972_cov_2.164553, whole genome shotgun sequence.
ACCESSION   DAWDRC010000501 DAWDRC010000000
VERSION     DAWDRC010000501.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12765_length_2972_cov_2.164553"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2259..2268
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          714 a          798 c          730 g          720 t
ORIGIN      
        1 aacggattct gacgctgccg cgcggatttt atttgaagaa gaatttcacc agtgcgctac
       61 tggcccgtca ttttctgatc cagtagccat cgctttgacc tgctgctttc cgggcatata
      121 attaccgctt catttttttg gcagggcttt ttagatgtta tttgcatgga taaccgatcc
      181 taacgcctgg cttgcgctcg gtacactgac gctgctggag atcgttcttg ggatcgacaa
      241 tattattttc ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg ctcatgcgcg
      301 ccgtctgggg ttggcgggag ccatggttat gcgtctggcg ctgctggcat ccatcgcctg
      361 ggttacgcgc ctgacgaatc cgctttttac aatattcagt caggaaattt ccgcccgtga
      421 tttgatcctg cttctgggtg gcttgttcct tatctggaaa gccagcaagg aaatccacga
      481 atccatcgaa ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc tcggcgctat
      541 cgtgcagatt atgctgctgg atattatctt cagcctcgac tcggtgatta ccgctgtggg
      601 tctgtcagat cacctgttta ttatgatggc tgccgtggtg attgccgtag gcgtgatgat
      661 gttcgccgcg cgctcgattg gtgattttgt cgagcgccat ccttcggtaa aaatgctggc
      721 gctctctttc ctgattctgg tgggctttac cctgattctg gaaagtttcg acatccacgt
      781 accgaaaggt tacatctact tcgcgatgtt cttctctatt gcggttgaaa gcctcaacct
      841 gattcgcaac aaaaagaatc cgctctgata ctccgtacgc tctcctgcgg gagggcgtac
      901 tcactcctgt taccctcaca gtttaagatt ttcctgcttt caagatatat aacgtcggtt
      961 tataaacaga ctattatcat aggtgggcca aatgaggata accggatgaa aaaatgggca
     1021 gtaataattt ctgcagtcgg actggcgttt gctgtttccg ggtgttccag tgattacgtc
     1081 atggcgacca aagatggccg tatgattttg accgatggaa aacctgaaat tgatgatgat
     1141 accgggctgg tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat
     1201 gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat ccggctggcc ttaagatttt
     1261 tctctttccc ttttcccttc cctctgccat ttttatattc cttatgtcgt gattataaaa
     1321 aggaaacggc tatgcaatat caccgtatac cccacagttc actgtaagtc agcacgctgg
     1381 ggcttggcac gatgacgttt ggtgaacaga acagcgaagc cgacgcccac gcacaactcg
     1441 actatgccgt cgctcagggc attaacctta tcgacgttgc cgaaatgtac ccagtacctc
     1501 cgcgccccga aactcaaggg ctaaccgaaa cctacgtcgg caactggctg gcgaaacatg
     1561 gcagccgcga aaagttaatt atcgcctcca aagtgagcgg acagtcgcgc aataatgaca
     1621 aaggcatccg cccggatcag gcgctggatc ggaagaatat ccgcgaagcg ctgcatgaca
     1681 gcctcaagcg tctgcagact gattacctcg atctctatca ggtgcactgg ccgcagcgcc
     1741 caaccaactg cttcggcaaa ctcggttata gctggacaga ttctgcgcct gcggtttcgc
     1801 tgctggatac gctggacgca ctggcagagt accaacgcgc gggaaaaatt cgttatatcg
     1861 gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct gcatctggcg gacaaacacg
     1921 atctgccgcg tattgtcacc attcagaacc cctacagcct gttaaaccgc agttttgaag
     1981 taggtctggc agaagtcagc cagtatgaag gggtcgaact gctggcctat tcgtgcctgg
     2041 gtttcggcac actgaccggg aaatatctca atggtgcaaa acccgctggc gcacgtaata
     2101 cgctctttag tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc gtcgcggcgt
     2161 atgttgatat cgccagacgt catggcctgg accctgctca gatggcgctc gcatttgtac
     2221 gccgtcaacc gtttgttgcc agcactctgc tgggcgcann nnnnnnnnaa ccacgatgga
     2281 gcagctgaaa actaacgtcg aaagtttgca tctggagttg agcgaagacg tattagctga
     2341 aattgaagcg gtgcatcagg tttatacgta tcctgcacca taagattaac gccggttaaa
     2401 accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca
     2461 aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat taccgccagc
     2521 gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc
     2581 gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg
     2641 acaaaaaagc cccccagcac gccaatcagc atcaacaaag cataggctgg cagcagctcg
     2701 tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa tcccggctgg catacagcgt
     2761 gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac gataccaatc
     2821 gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatacccag cgccaccggt
     2881 acccacagca ccaacaggaa acgcagcgtg acacctgctc cccagaataa actggtgccc
     2941 accagcgaaa aacgcgtttc accattgcgc ca
//
LOCUS       DAWDRC010000502         2963 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12795_length_2963_cov_2.155777, whole genome shotgun sequence.
ACCESSION   DAWDRC010000502 DAWDRC010000000
VERSION     DAWDRC010000502.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12795_length_2963_cov_2.155777"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2730..2739
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          647 a          802 c          794 g          710 t
ORIGIN      
        1 gcggcgcaat ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt
       61 ggtcagcaaa gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag
      121 gtgctgacgg tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca
      181 gcggaagaag cacggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag
      241 gcactgcgcg ttgctattcc ggcactgatt gtctcgctgt tcgtcagcgc tgatatggtg
      301 agcaatatgc tgagcgccat tcccgaattt gtgacccgtg gactgcagat tgctggcggt
      361 tttatcgtgg tggtcggtta cgccatggtg cttcgcatga tgggcgtgaa atatttgatg
      421 cctttctttt tcctcggttt cctcgcaggt ggctacctcg atctcagtct gctggcgttc
      481 ggtggcgtcg gcgtgatcat ggccctgctc tacatccagt taaatccaca gtggcgtaaa
      541 gctgaaccac atccccagac caccactatc accgcccttg accaacttga tgattaacgg
      601 agccatcatg gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc
      661 caacctgcaa caggcatcct ttaactttga acgtattcac gggctgggtt tttgctacga
      721 catgatcccc gccatcaaac gactttaccc attaaaagag gatcaggttg cggcgctcag
      781 gcgacacctg gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgtcac
      841 tgccgccatg gaagaggcgc gagccaacgg cgcggaaatt gatgacggta ccattaacgg
      901 catcaaagta ggtctgatgg gaccattggc aggagttggc gatccactgg tctggggaac
      961 gctgcgcccg attaccgccg cgctcggcgc atctctggca ctttcgggca acattctcgg
     1021 ccccctgctg ttcttcttta ttttcaacgc ggtgcgtctg gcgatgaagt ggtatggcct
     1081 acagctcggc tttcgcaaag gggtgaatat cgtcagcgat atgggcggta atttgctgca
     1141 aaaactcacc gaaggcgcgt cgattctcgg gctgtttgtg atgggcgtgc tggtgaccaa
     1201 atggacgtca atcaacgtac cgttggtggt ttcacaaacg cctgccgccg atggtgccac
     1261 cgtcaccata accgtgcaga acatcctcga ccaactttgc cctggtttgc tggcgctcgg
     1321 tctgacgctg ctgatggttc gtctgctcaa caaaaaaatt aacccggtat ggctgatttt
     1381 cgccctgttt ggtttaggga ttatcggtaa tgcgctgggc ttcctgtcct gattcttcgc
     1441 cccggcgcaa ccgtcggggc catcgttcaa catgaggtgg tttatgaaaa caacagctct
     1501 gcgtctttat ggcaaacgtg acctgcgtct ggaaaccttt gaccttcctg aaatgcagga
     1561 ggatgaaatc ctcgcaacgg tggtcactga cagcctgtgc ctctcttcct ggaaagaggc
     1621 caatctgggt gaaaaccata aaaaagtacc cgacgatgtg gcgaccaatc ccatcatcat
     1681 cggccacgaa ttttgcggcg atattctggc cgtgggtaaa aagtggcagc acaaattcca
     1741 gccgggtcag cgttatgtga ttcaggccaa cctgcaactc cccgaccgcc cggattgccc
     1801 cggctactcc ttcccgtggg taggcggcga agcgacgcat gtggttattc ccaacgaggt
     1861 catggaacaa gattgcctgc tggcatacga cggcgaaacc tattttgaag gctcgctggt
     1921 tgaaccgctt tcctgcgtga ttggcgcgtt caacgccaac tatcatcttc aggaaggtag
     1981 ttataaccac acgatgggga ttcgcccgca agggcgcacg ctggtcctcg gcggcaccgg
     2041 cccgatgggg ctgctggcga ttgattatgc gctacatgga cccgttaacc cttcgctgct
     2101 cgtcattacc gataccgaca acgacaaatt gagttatgcg cgcaagcact atccatcaga
     2161 accgcaaaca ctgattcatt atctcaatgc caccgatgca gcatttgata cgttaatggc
     2221 actgagtggc ggtcacggct tcgatgatat tttcgtcttt gtgcctaatg aaggactggt
     2281 gactctcgcc tcttccttgc tggcgacaga tggttgtctg aatttcttcg ccggaccgca
     2341 ggataagcac ttcagcgcgc caattaattt ctacgatgtg cattatgcat ttacccacta
     2401 cgtgggcaca tcaggcggca ataccgacga catgcgcgca gcggtcaaat tgattgaaga
     2461 gaaaaaagtg caggccgcaa aagtggtaac acatattctt gggctgaatg ccgcgggcga
     2521 aaccacgctt gaattgcctg ccatcggcgg cggcaaaaag ctggtgtata ccgggaaata
     2581 cctgccgctg acgtcactca cgcagattca ggatcaagca ctggcggcaa ttctggcgcg
     2641 tcatcagggg atctggtccg gtgaggcgga gcaatacctg ctcgcccatg cagaggcaat
     2701 ttcccatgat taatcgcgct acttaactcn nnnnnnnnna actctgcatg tcgctggctg
     2761 gtcgccgcgg caacttcgcg acctgatgtg cggctgattg ctgaagcggc acaatgtgca
     2821 cgagaagaat aaaaaaacat cagcgcctta aggggcgctc aaattggtgg caaagtgcac
     2881 attgtttatg ccggatgcgg aatgaagcct tattcgccta cataatcttg aaaattcaat
     2941 atattgcagt tatctggtag gcc
//
LOCUS       DAWDRC010000503         2958 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12825_length_2958_cov_2.077506, whole genome shotgun sequence.
ACCESSION   DAWDRC010000503 DAWDRC010000000
VERSION     DAWDRC010000503.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12825_length_2958_cov_2.077506"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2521..2530
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          766 a          660 c          713 g          809 t
ORIGIN      
        1 tttcaggtta tgcaagatct tacggataaa taactctttc tgcgctaact aaggaaaatc
       61 gcgatcaaaa acaaactatg acatgcaata ttcctggaaa cataaacttt atgccatgta
      121 cccagggaaa atcatcttca gtatagtaat tatgtaaacc gtcggagaac aatacgtacg
      181 gtaacgaaat tatctttcag caaggagctg tgaaaatgtc tcaaaataag gctttcagca
      241 cgccatttat cctggctgtt ctttgtattt acttcagcta tttcctgcac ggcattagtg
      301 ttattacgct tgcccaaaac atgacatctt tggcagaaaa gttttccact gacaacgcgg
      361 gcattgccta tttaatttcc ggtatcggat tggggcgatt gatcagtatt ttattcttcg
      421 gagtgatctc cgataagttt ggtcgtcggg cggtgatatt aatggcagta ataatgtatc
      481 tgctattctt ctttggtatt cctgcttgcc cgaatttaac tctcgcctac tgtctggcag
      541 tgtgcgtagg tatcgctaac tcagcgctgg atacgggtgg ctaccccgcg ctcatggaat
      601 gctttccgaa agcctctggt tcggcggtca tactggttaa agcgatggtg tcattcgggc
      661 aaatgttcta cccaatgctg gtgagctata tgttgctcaa taacatctgg tacggctatg
      721 ggctgattat tccgggtatt ctgtttgtac tgatcacgct gatgctgttg aaaagcaaat
      781 tccccagcca gttggtggac gccagcgtag ctaatgaatt accgcaaatg aacagcaaac
      841 cgttagtctg gctggaaggt gtttcatcag tactgtttgg tgtagccgca ttctcgacct
      901 tttatgtgat tgtggtgtgg atgcccaaat atgcgatggc ttttgctggt atgtcagaag
      961 ctgaggcatt aaaatccatc tcttatgaca gtatgggttc gctggtctgt gtctttattt
     1021 ttgccgcact actgaaaaaa atggtccgtc ccatctgggc taatgtattt aactctgcac
     1081 tggcaacaat aacagctgcc attatctacc tgtacccttc tccattggtg tgtaatgccg
     1141 gagcctttgt tatcggtttc tcagcagctg gcggcatttt acagctcggc gtttcggtca
     1201 tgtcagagtt tttccccaaa agcaaagcca aagtcaccag tatatatatg atgatgggtg
     1261 gactggctaa ctttgttatt ccgctgatta ccggttatct gtcgaacatc ggcctgcaat
     1321 atatcattgt tctcgatttt actttcgcgc tgctggccct gattaccgca attattgttt
     1381 ttatccgcta ttaccgcgtt ttcattattc ctgaaaatga tgtgcggttt ggcgagcata
     1441 aatttagcac ccggttaaac acgattaagc atagaggtta aaggagttaa ttatggatgt
     1501 taccgcaaaa tacgaattga ttgggttgat ggcctatccc atccgccaca gtttatcgcc
     1561 cgaaatgcag aataaagcct tagaaaaagc gggattgcca tttacctata tggcctttga
     1621 agtggataac gatagttttc ccggagcaat tgaaggatta aaagccctca aaatgcgcgg
     1681 aactggtgta tcaatgccga acaaacaact ggcgtgtgaa tatgttgatg aattaacgcc
     1741 agcagccaaa ctggtgggtg ccatcaacac catcgtaaat gatgatggct atctgcgtgg
     1801 ctataacacc gacggcacgg gccatattcg cgccattaaa gagagcggtt ttgatatcaa
     1861 aggcaaaacg atggtgctgt taggggccgg aggtgcctca acggcaattg gcgcgcaggg
     1921 ggcaattgaa ggtttaaaag aaattaaact ctttaaccgt cgggatgagt tcttcgataa
     1981 agccctcgcc ttcgcgcagc gggttaacga aaacaccgat tgcgtcgtga cagtcaccga
     2041 tctcgccgat cagcaagcct ttgctgaagc cctggcttcc gccgacattt taaccaatgg
     2101 caccaaagtg ggtatggaac cccttgagaa tgaatcattg gttaatgata tcagtctgtt
     2161 acacccggga cttctggtca ctgaatgcgt gtataaccct catatgacga agttattgca
     2221 gcaggcgcaa caagcgggtt gcaaaacgat tgatggatac ggcatgttgt tgtggcaagg
     2281 ggctgaacag ttcacgttat ggactggcaa agatttccct ctggaatatg ttaaacaggt
     2341 catggggttc ggtgcctgac aggctgaccg cgtgcagaaa gggtaaaaaa tgaaaaccgt
     2401 aactgtaaaa gatctcgtca ttggtacggg cgcacctaaa atcatcgtct cgctgatggc
     2461 gaaagatatc gccagcgtga aatccgaagc tctcgcctat cgtgaagcgg actttgatat
     2521 nnnnnnnnnn gagtctgtca tggcggcagc aaaaattctc cgtgaaacca tgccagaaaa
     2581 accgctgctg tttaccttcc gcagtgccaa agaaggcggc gagcaggcga tttccaccga
     2641 ggcttatatt gcactcaatc gtgcagccat cgacagcggc ctggttgata tgatcgatct
     2701 ggagttattt accggcgatg atcaggttaa agaaaccgtc gcctacgccc acgcgcatga
     2761 tgtgaaagtt gtcatgtcca accatgactt ccataaaacg ccggaagccg aagaaatcat
     2821 tgcccgtctg cgcaaaatgc agtccttcga cgccgatatt cctaagattg cgctgatgcc
     2881 gcaaagtacc agcgatgtgc tgacgttgct tgccgcgacc ctggagatgc aggagcagta
     2941 tgccgatcgt ccaatcat
//
LOCUS       DAWDRC010000504         2956 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12836_length_2956_cov_1.729059, whole genome shotgun sequence.
ACCESSION   DAWDRC010000504 DAWDRC010000000
VERSION     DAWDRC010000504.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12836_length_2956_cov_1.729059"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1303..1312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1800..1809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2707..2716
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          726 a          783 c          747 g          670 t
ORIGIN      
        1 gggggttaaa agaagtgatt aatgcgcccg caggcgcatt aatgattact cagcgaggaa
       61 taattcaaga agtgagttta agaataattt accatgctcg gttatctgcc agtaatccgc
      121 acattctgtg agataaccct gagcgatagc ctcgtctaac tgtgggcgaa taacctcttc
      181 tgaaaggcca gtatactggc taaactccac gcgcggcgca ggttccagca aacggaagcg
      241 attcataaag aactcaaatg gtttatctgc ggcttcgaca tcacgctggc tttccagata
      301 ccgcccctgc ataaaaccac gtggatgacg cgttttggtg gtacgcagaa tgcgcccatc
      361 cgggaaggtc actttgccat gcgcgccgca gccaatacca atgtagtcac caaagcgcca
      421 gtagttgaga ttgtgctggc actgataacc gggtttggcg taagcggaag tttcatattg
      481 ctgataaccc gctgcggtta ataactgatg cccctgttcg aaaatgtccc acagcgcgtc
      541 atcgtccggc agtacaggag ggcgcgagcc aaacagcgta ttgggttcga tggtcagttg
      601 ataccaggaa agatgcggcg gattcagttc aatagcctgg cgcagatcgc ctagcgcctc
      661 ttccagtgat tgatccggta gcccatgcat caaatcgagg ttaaagctac gtaaccctaa
      721 accgctcgcc agatttgccg ctcgtttcgc ttcttgcggg ccatgaatgc gcccaagtcg
      781 tttcagcttt tcttcgctaa aactctgtac gccaatggag atgcggttca caccagcacg
      841 ctgataatca acaaagcgat cggcttcaac cgtaccaggg ttcgcttcca tcgtaatttc
      901 tgcatccgct gccagcggca aacgcgcacg cacgccgttc agcagcgttt gcatcgccgg
      961 gccggaaagc aggctcggcg taccaccgcc aataaagatt gtctttactt cacggccctg
     1021 agcgtaagcc acatcgttgt ccagatcgtt aagcagatgc tgaacataat cgtcgtgcgg
     1081 cacttctcct ttcaacgcgt gagagttgaa atcgcagtac gggcatttct gcacgcacca
     1141 cgggatgtga atgtagagac tcagcggcgg taatttaacc attacgtaaa gcgtccagca
     1201 gcagtttcaa cgcctgacca cggtgggaaa tggcgctctt ttcttcgcgg gtcagttcgg
     1261 cagcggtttt cccttcggaa ggtacgaaga agattggatc atnnnnnnnn nncgtgacgc
     1321 agatatacca gcacgcagtg gaaacgcgcc tgacgttggt cgtccggtac gtctttcatt
     1381 gtttccagca gtttttgcag attcttttga tcggtcgcgt cttcaccgga ataacgcgcg
     1441 gagtaaatac ccggcgcgcc acccagcgca tctaccgcca gaccggagtc gtcggcaatt
     1501 gccggtaaac cggtcacttt tgccgcatgg cgcgctttca gaatcgcgtt ttcgataaag
     1561 gtcaggccgg tttcttcagc ggaatctacg ccgaggtctg tttgggcaac gatatcaaga
     1621 ccgaagtcgc taagcagcga cgccagctca cgcactttac cggcattgcc ggttgcgagg
     1681 acaacttttt gcataggata cctaattaat taacgccgcg atttctggcg ggatttgttg
     1741 cggattaatg attttaattt gtttgtggcg gccaagttcg cctttttcaa tcaccacctn
     1801 nnnnnnnnnt ggcctggccg tcaaccggcg gcgcggtaat ggcgactttt acctcgtcgc
     1861 catgtaaacc gacaatagaa tcacggctgg cttttggctg aatatagagc cgtaaaacca
     1921 gaccgtcatc attaactgtt acggcactca taacgccatc cacagccccg gcagcagcat
     1981 atttccggtc gcctgcaaga cttctgcgac acccatattg atgacataca gcagcagaac
     2041 gaggatcatc ggcgagaaat caatcccacc cattgccggt agcaagcgga tcgggcgcag
     2101 cagcggatcg gccagctgaa tcagcacgta ttcaatcggg ctacgaccct ggcttaccca
     2161 gctcataatc gccatcacca gcaggaccca gaaaatcagc aggccgatgg ttttcagcag
     2221 aatcaataaa ccggcaatcc aaatgattgg caggaaggtc accactttaa acagcacgat
     2281 ggctttaatg aaacttagaa tataggcaac cagcagcgag gcgctatcaa tggggcccat
     2341 tgccggaata acgcggcgca gtggcccgat aatcggctgc gttaccttca ctacaaactg
     2401 tgagaagggg ttgtaaaaat cacaatgagc ccactgcatc cagatgcgta ataacagcac
     2461 catggtatac agctcaatga ccgttgaaag caggaaagtc aacgtattca tggcgttcct
     2521 ttaattcctt attttttaga gtaatcacgc gcaccaaaaa ttgcagtgcc gatacgaacc
     2581 atcgtgctac ctgccgcaat ggcggcttcc atatcgtccg acattcccag agagagcgtg
     2641 tcgatatgcg ggtagcgcgt tttcagtccg gcaaatgcta cagccatttg gcgtgcaact
     2701 tcaaacnnnn nnnnnngact caggcgcagg gattgccatc agcccgcgca gacgtaaacg
     2761 cggtagttca gcgaccgcag ctgccagctc gtccagttca gccagttgaa tcccggactt
     2821 actgttttca tcactaatgt taatttgaat cagtacgtta agagggggaa gttctgccgg
     2881 gcgctggtcg ttcaggcggg tagcgatgcg caaacggtcg atggtatgac accagtcgaa
     2941 atgctctgcc accagg
//
LOCUS       DAWDRC010000505         2952 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12855_length_2952_cov_1.966517, whole genome shotgun sequence.
ACCESSION   DAWDRC010000505 DAWDRC010000000
VERSION     DAWDRC010000505.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12855_length_2952_cov_1.966517"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          684 a          738 c          726 g          804 t
ORIGIN      
        1 ataaatgaga taatttacag tgagctaaaa atcagccagg cgataatgac ccggctgaca
       61 gctttagtct ttagctgttg cattttgttt atgaaacagt tcacggaata ccggataaat
      121 atcatcctgg tcgcggatat gctgcatcgc aaagttgtcg aaagtagatt gcagatgctc
      181 atattctcgc cacagtgtct gatgcgcgcg acgtgtaatt tcgatatagc tgtaataacg
      241 aacaacgggt aatatttttt tcgccagaat ttcatggcaa agcggagagt catcggccca
      301 gttatcgccg tctgatgctt gggctgcgta aatattccac tgtgccgggt tataacgctc
      361 tttcactacc tcatccatca gttttagggc gctggaaaca atggtgccgc cagtttcctg
      421 cgagtagaaa aactcatgtt catcgacttc tttcgcctgg gtatgatggc ggatgtatac
      481 gacttccacg ttcttatacg ttctgctgag gaacagatac agcagaatat aaaaacgttt
      541 agccatatct ttagtggact gatccattga accggaaacg tccatcaggc aaaacatcac
      601 tgcctggctg gagggatcgg gccgtttttc gtagttcttg taacgtaaat cgaaagtgtc
      661 aatgaaagga acgcgttcaa ttttggcacg taattctgca atttctttgc gccgttcctc
      721 ttccagcagt tgcgcaggtt cactgttgct gatgatggcc aaattctctt ccagtgcatg
      781 aagttcccgc cgcttgcccg ccgtcatggc tgtgcgccgc gccagtgagt tctgcaatga
      841 acgtacaacg ctgatattgg ccggaacgcc gttagcggta taacccgccc gatgcgtttt
      901 atattcggtc agctggcgtt gttggttttg tttcagattc ggtaaggcca aatcttcaaa
      961 gagcagatca agatactcat ctttcgaaat ctgaaagaca aattcatcct gaccttcacc
     1021 atcctggctg gcctggccct gaccactgcc ggaacctccg ccaccaccct ggggacgttc
     1081 aattcggtcg ttctggacga aatggtcatt gcccggatgc acgcggtggc gcaggcctcc
     1141 acgcccctga tgaaacatcg gttcgctaat atcttccgtg ggaatggata cggactcgcc
     1201 gctgtcgacg tcagtcaccg aacgcttatt aatggcctcg gagatcgact gtttaatttg
     1261 cgctttataa cggcgtaaga aacgctggcg attcaccatg cttttatttt tgccgttcag
     1321 acgccggtca ataaaccagg tcatatgccc cccgtactac atttgccaac ggtacgctac
     1381 gcctgtgttg ccgcttctct ggcccacggt tcgctgtcgt tgtaggcccg gtagcatgtg
     1441 taccgggcca gggttgttaa gacgatttac gtacgcgcaa ataccattcg cacagtaaac
     1501 gcacctgttt acgggtgtag cctttttcca tcatacggtc gacaaagtcg tcgtgtttct
     1561 tctgctcgtc ggttgacgtt ttggcgttaa acgaaataac cggcaacagc tcctcggtat
     1621 tagagaacat tttcttctca ataaccgtgc gcagtttttc atagctggtc cagttcggat
     1681 tacgtccgct gttattggct ctggctcgca gtacgaagtt aacaatctcg ttgcggaaat
     1741 cttttggatt actgatcccc gccggtttct cgattttctc cagctcggcg ttaagagact
     1801 cgcggtcaaa cagctgcccg gtatccggat cgcgatactc ctgatcctga atccagaaat
     1861 ccgcgtaggt aacataacgg tcaaaaatgt tctgcccata ttctgaatag gattcaaggt
     1921 aggccgtctg gatctctttg ccgataaact cggcatattt cgggatcaga taacctttca
     1981 ggaactccag atagcgttct gcctgctctt gcgggaactg ctcgcgctcg atctgctgtt
     2041 ccaggacgta gaacagatgg accgggtttg ccgccacttc tacatgatcg aagttgaaca
     2101 cgcgggagag gatcttaaac gcaaaacgcg tcgacaggcc gttcatccct tcatcgacac
     2161 cggcgtagtc acgatattcc tgatacgact tggctttggg atcagtgtct ttcaaacttt
     2221 caccatcata aacccgcatc tttgaataaa tgctggagtt ttctggctct ttcagacgcg
     2281 aaagaataga aaaacgtgac agcgtttcca gcgtgccagg ggcgcagggg gcgtgagtca
     2341 attcactgtg attaagcaat ttctcgtaga ttttgatctc ttcggaaatg cgcaagcaat
     2401 acggcacctt cacgatgtaa acacggtcga ggaaggcttc gttgtttttg ttattacgga
     2461 aagtgaccca ttcggattcg ttcgagtggg cgagaataat cccgttgaac ggcagggcgg
     2521 agataccttc cgtcccgttg tagttacctt cctgggtggc ggttaacaag ggatgcagca
     2581 cttttatcgg tgctttaaac atctcgacaa attccatgat cccctgattg gcgcggcaca
     2641 gcgcaccgga atagccgtag gcgtccgggt cattctgcgc gtagtgttcg agtttacgaa
     2701 tatcgacttt ccctaccagg gcggagatgt cctggttgtt ctcatcgccg ggttctgttt
     2761 tggcgatagc aatttgttgc agaattgacg gccagacctt cactacccgg aacttagtga
     2821 tatcgccacc aaattcatgc aggcgttttg ccgcccacgg cgacatgatg gtgccgagat
     2881 aacggcgagg aatgccatac tctttttcca gaatctgcgc atcttcctgc ggattgaaaa
     2941 gacagaacgg at
//
LOCUS       DAWDRC010000506         2952 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12853_length_2952_cov_2.242665, whole genome shotgun sequence.
ACCESSION   DAWDRC010000506 DAWDRC010000000
VERSION     DAWDRC010000506.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12853_length_2952_cov_2.242665"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2016..2025
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          767 a          738 c          574 g          863 t
ORIGIN      
        1 ggtgatttat cagttattat gtaattaata tgactaaagt cagctaactg gacaaaagct
       61 ttgcgatcaa acttagagtg atcaaccaat aacgcaactt ctgtcgcctg acggatcatt
      121 gtctttttga tttcagcttc cgcttcatta gagtccagcg cgccgctgtt aatatcaaga
      181 cctttacaac tcattaccat gatatcgaca tggtagcggc tgatgatctc tttagtaatt
      241 cttccttgca gtgaaagtgt atttttgttt agttccccac ccgttgaaac gactttaatt
      301 tctgactgag ccagaacatg aatcgcttcc gccgagttgg ttagcaacgt caggtcacta
      361 cggtcctgta acaatttgag caattccatt acggtactac tggaatcggc tgccatggtg
      421 gttttattgt cgacaaaggg tagtgccttg cgtgcaataa gctgcttctc ttcataaaac
      481 gatgaagcgc gcttataaaa atggatattc tccgtcaaca tcgctgtatt taaaacagca
      541 ccaccatagg ttctggtcaa aaagccttca tcttccaact tctcaagatc gcggcgaatg
      601 gtttcttcgg ttacctgaaa aatcccactc aaatttgaga ctgtcacctt tttatcgttg
      661 gcaaccattt gcttaattgc ctgaatcctg tcttttgccg ccacgattac acccctgtat
      721 ctttttacat cacattagcg caattatcgc ataaccgatg tttactttca aaataacctg
      781 tttgaatcac agattttcat cacagttttc acagaaacag aggtgaatcg tgttgagtat
      841 ttaacgctcc agggcctctg ccatttccct catcaatgtt gcgtctccat ccgataagtt
      901 gatattcagt gccgcgacat tttcacgtac ctgttccggc gcagtagccc cactaagaat
      961 ggagattaaa tcactctgtt ttaatatcca cgccagtgcc agagtgggaa ttgtgcactg
     1021 ataacgagca cagagtggct gccactgttc aagcatatca atcactttca gcatgttttc
     1081 acgctggaac cagactttat ttgcccgagc gccgcccgga acgtaatcac gcgtgatggt
     1141 cccggtcaac aatccctgct ctagcggaga gtagacctgt accacaatgc cattatcacg
     1201 acatagcggc agcagttcgt tttccattgc ccggtcgagg atactgtatt tcgcctgaat
     1261 aatatccagt tcaccatgtt gcagatactc gcggatatgg tcagcatcga cgttagcagc
     1321 gcctatagcg cgaattttcc cttcggcctt taacgcatta agcactgcga cagtttcagc
     1381 tatcggcgta aaaaatggcg gcaccgactg ccagtgcgtc atgtagatat cgatgtaatc
     1441 aatacccaga cgttgcaagc tggcttctac ctcttcgcgg atagattccg gagaaaggtt
     1501 tttatacaac tgccgatcgc caactttgtt gaataaactc ccttttcgtt cccagacaat
     1561 gccgcatttg gtttctacta caacctgttc acggggcagt ttttttaacg cctgaccaac
     1621 gataacttca ctattgccaa agttatatcc tggcgcagta tcaatcaggt taatgccgca
     1681 acgatgggct tcaagaatcg tatcaatgca tatttgtcga tcgagatcgc cattccatgc
     1741 aggaccgccg ccaatagccc atgtacccaa ccccattcgc gaaagcgtta tatccgctgt
     1801 gcctaaaggt atctttttca tttgccttca ttatccttcg tattcttcca gcaattgttc
     1861 gaccagcttt ctgtttttta caccggtggt ggcgccgatg cttagaaccg agatagccgc
     1921 cgttgcattg gcaaagcgtg cgcattcacg cagattttta ccttctaaca gtgctgcaat
     1981 aaaacctgaa gcaaagttat cgcccgcacc aatggnnnnn nnnnnttcat cgtcatgtca
     2041 ccacgcttga taaagcagcc gtctttaccc gttttaatca ccaccgtttt tacgccgcac
     2101 gcaagaaagc agtcagcaat ttcatccagt gtctctttcc cggtgagtaa ttttgcctcg
     2161 gcaaaattag gaaacagata atcgacataa ctcaatgctt cgcaaatatc attcagcgtt
     2221 tcattcaacc gcggtttgat catatcggca cagataatca tctgccgggc tttagcttgc
     2281 gtaaaaattt ctgttagcgc tctaccatcc aatagaggac tgttgaaaat actggccagt
     2341 gataataatt tcgcctgaga aaaccgcgca aaatcaacat cgtcaatatt caatttccac
     2401 agactgccat tacggtttgt gacaaaggtc cgctcgccat cctccgtcac cagtccaacg
     2461 ttaatagagg tatctatgct gacgtcctgc ttcaggcttt gaatatcaat attctctttg
     2521 cggcaatggt cgagaataaa ttgccctgcg gcatctttac caatacgact cattaatgct
     2581 gtgcgatggc ccagacgaga aataattgtt gcttcattta ttgcatcccc cccggtggtc
     2641 attgcgattc tttcaagagg ataagaatcc acatcaaaga tatttttact gaccggttgc
     2701 aatggaatat caacaatagc ggcacctata caaataacgt cgagattatc catattatta
     2761 ttccgctttg ccatcagaac cgaacagttt aattttatcc aatgcccgtt cttttacagc
     2821 tttacgcact tcacgttcta aatgcaggaa aggctgatcc tggttttctt tcactgctac
     2881 cattgcggcc tgacagagct ccgtatgaat gttgattttc gcaataccca acgaaagtgc
     2941 agttttaatg tc
//
LOCUS       DAWDRC010000507         2942 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12886_length_2942_cov_2.316938, whole genome shotgun sequence.
ACCESSION   DAWDRC010000507 DAWDRC010000000
VERSION     DAWDRC010000507.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12886_length_2942_cov_2.316938"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2413..2422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          654 a          712 c          764 g          802 t
ORIGIN      
        1 aataacaatt aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac
       61 actttcgaga ggattcagga tggggcaatc atcgcaacca catgagttag gcggcgggct
      121 gaagtcacgc cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt
      181 cggttccagc gtcgccatcg ccgaagcggg cccggcggta ttactggcct atctgttcgc
      241 cggattactg gtggttatga ttatgcggat gttggcggaa atggcggttg ccacgcccga
      301 taccggttca ttttccacct atgccgataa agccattggc cgctgggcgg gctataccat
      361 cggctggttg tactggtggt tttgggtact ggttatccca cttgaagcca acatcgccgc
      421 catgatcctg cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac
      481 cctcgcctta accggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg
      541 gctggcgctg tgtaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat
      601 taacggtttt tacccgtatg ccgacgtgag cggtatttca agattgtggg atagcggcgg
      661 ctttatgccc aacggcttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt
      721 tatgggcgca gaaattgtca ccattgccgc cgcggaatcc gacacgccgg aaaaacatat
      781 tgtccgcgcc accaactcgg ttatctggcg tatctctatt ttctatttgt gttctatttt
      841 tgtcgttgtg gcattaatcc cgtggaatat gccggggctg aaagccgttg gttcttatcg
      901 ctcggttctg gaattgctca atattcctca tgcgaaatta atcatggact gcgtgatatt
      961 actttccgta accagttgcc tgaactcggc gctgtatacc gcttccagga tgctctactc
     1021 cttaagccgt cgcggtgatg ctcccgcggt aatgggtaaa atcaaccgca gtaaaacccc
     1081 gtacgtagcg gtgttactct ccaccagcgc ggcattctta acggtggtgg tgaactatta
     1141 cgcgcctgcg aaggtgttta aatttctgat cgacagctcc ggcgctatcg ccctgctggt
     1201 ttatttagtc atcgccgttt cacaattgcg gatgcgcaaa attctgcgag cagaaggaag
     1261 cgaaattcgc ctgcggatgt ggctttatcc gtggctcacc tggctggtaa tcggttttat
     1321 tacctttgtg ttggtagtga tgctattccg cccggcgcaa cagttagaag tgctctccac
     1381 cggcttatta gcgattggga ttatctgtac cgtgccaatt atggctcgct ggaaaaagct
     1441 gataatgtgg caaaaaacac ccattcataa tacgcgctga taatgatcag gagtcacacc
     1501 atgaccatta cctctctgga tggctatcgc tggctgaaga acgatattat tcgcggcaat
     1561 tttcagccgg atgaaaaatt acggatgagt ttgctgacat cgcgttatgc acttggcgtt
     1621 gggccgttgc gggaagcgct ttcgcaactg gtggcagaac gactggtcac ggtggtgaat
     1681 caaaaagggt atcgggtggc gtctatgtca gagcaggagt tgctcgatat tttcgatgcc
     1741 cgtgccaata tggaagcgat gctggtaagc ctggcgattg cccgcggtag tgatgagtgg
     1801 gaggcagacg ttcttgcgaa ggcgcatctg ctcagtaagc ttgaggcctg tgacgccagt
     1861 gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt ttcatactgc aattgtggcg
     1921 ggctgtggtt cttactattt gctgcaaatg cgtgaacggt tgtttgatct ggcagcgcgt
     1981 tatcgattta tctggctgcg gcgaacagtg ctttcggtgg aaatgctgga agataaacac
     2041 gatcagcacc agaccctgac tgcggcggta ctggcgcgag ataccgcgcg cgccagtgag
     2101 ttaatgcgcc agcatttact gacgccaatt cccattatcc agcaggcgat ggctggcaat
     2161 taactactct tcaggaatac gcaacacttg ccccgggtaa attttatccg ggctttttag
     2221 catcggttta ttcgcttcga agattttatt gtacagatta gcgttaccgt agacctgttt
     2281 ggaaatggca ctcagagtgt cgccagactt aacggtataa aactggctgg cagtggctgg
     2341 tgtcgccgtt ttcacctgat catcgacact ggcaataccg gaaatattcc ccaccgcaac
     2401 aaggattttc tcnnnnnnnn nnatttgaat gttcacttta tcggcatcag gtataccggt
     2461 tttgttcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct gacctgtaac
     2521 cgcgtcccag agtttttctc cggcatcttt cacaaaattg aacagaccca tagctacctc
     2581 attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt aacgccgcgg
     2641 gggtgagcac aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa accggggata
     2701 tagccaagca gtgtcagcag aatattaata atgaacgccc aaccgaaccc tttaccgagc
     2761 agcacgccga gcggcggcag aatgatggtg atgacgattc tccagaaacc catatgtact
     2821 ccctataaga aaattactca ttgtttaaaa agagatttta tctcttaagc gtagtaattt
     2881 tagccgagcc tgccagtttc agcttacgct tttactctca tttacgctgc tgatgctggt
     2941 at
//
LOCUS       DAWDRC010000508         2937 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12913_length_2937_cov_2.671756, whole genome shotgun sequence.
ACCESSION   DAWDRC010000508 DAWDRC010000000
VERSION     DAWDRC010000508.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12913_length_2937_cov_2.671756"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          712 a          749 c          638 g          838 t
ORIGIN      
        1 tcgggtttac gataacattt cctgtaaatc tgccgctact aacagtcaac cggccacctg
       61 gggaagaaac tgtcgcattt ttaaaagtac caccatacac agtcacctgg ccgctcatga
      121 tcaacagatt tccaagatca gaatcttcgc tggcataaat agtagcaatg ccgggagtgt
      181 aatatgtgcc atagcttcca tgcaccacaa tgcttggggc agtgtctgaa tacgtgttct
      241 ggatcgtata ttttccgctg ccaatttcaa gggaaccacc atactgcaca taaatcgtcc
      301 cccaatcccg cgattctcca atcagcgtgc caccagggct atcgcctact gccctgaaat
      361 tcagcttcct tctgtccttt ggcacagaga acaccgttcc actgctcgta agcgttttgc
      421 cgttcattac catgtagagt gtatgcccac cggtgacata ctcgttgtta agggcacctc
      481 caacagttgc atcctggaac atggtaaacc agcaatcttt ggatttcagc atatcccagg
      541 ctgcgtttat tgcgaggtca aaggtttcat aatactttct gtcagttacg taggaagtcg
      601 gagacacact ggccgccatt tgcgagttgc atctgttgca gtaaccagta tcctgattaa
      661 cccggtcatg cgtacaagtt ttgaatgtct tttcaagttt ctttataaca taaaaatcgc
      721 cgtatttaat ctgcgcacaa atggtgcatt cagatacttt tggagtataa tcagtcgtaa
      781 atgtttgatt ggtatcgagt ctaaaacttc cgagccactc actattcgtc gtaccagaac
      841 tcgcagaagt tttaacagaa tattcgcaag tagcttcact accgtccgtg agaccgtcca
      901 gtgttacaga aagcgtcacc tttcggttta cataagtatc ttccggagtg accgtaatat
      961 ctgaggttga gaagggatac tccgcaaccg tgacattttc gatatatgta ctgtaaagtt
     1021 gattcaagtt cgcccagttc tcatttactt tatatcccat cttagggcct aaaatatatc
     1081 cgaccccgct atagctcata agataatcat acttactgcc tatcgtgagc gacagcgtag
     1141 ctgttttgct ttgcaagtaa aatctgccat tattttcgct cgccgtagaa cgatcatttc
     1201 ttacggtcag gttggccgta ccgccgaccc caaaggaatt tatttgtttt agcgtatgcc
     1261 cattaaaatc gagcgtaaag aagcccttct gaagctcata gctgccgagg ctgcaatcag
     1321 caagcagctt cacagtgcag cccgaatttg cctctgtctg cgctgcactc agtgcttctt
     1381 ccaatgtctc ataataatac gtggcggagc tggacgcgcc agcggtaagc tccgccacga
     1441 gtgcggcgcc gcactcgtta catttaactt tgccgttaac atcgattgcc tctaaatgcg
     1501 aacacacgcc aaactgcaac gttcgttcag ccgtggtttc ttgattgtta taggtaatct
     1561 ggtatttaac actatggctc tcactcgata catcaaaggt gatagtgatt agaagcgtat
     1621 cctcgccttc accgccaaca tcagcaaatg taatgttatt gttgtcaact tgcagtgtac
     1681 tttgctgcgc agcctttata atatcatttc tcagattaac atctgacaga tgaattcgaa
     1741 cagttaatgt cacttcacca ccactaagca caccgggctt ttcatcagtg atgagattac
     1801 cattttgtgt tgcagtgact gagacagtaa acggtgtatc ctctgccagc ggtatgaaca
     1861 gattccctac tgtcatcagc tccggctggt tttccgcctc tggttccttt tcaggctgct
     1921 caatcggcga ctcttcctgc tcctgtttcg gctcctgctc aggctctttc tgctccggtt
     1981 cttcggtcgg attttcttcc ggctgctcct ccggctttgg ggccggtgtc tctttttctt
     2041 cggcctccgg ctccggctca ttcatagacg aagcgggccc ctccggcttc tgcacgtctt
     2101 cggccagcgc cgccatcggc agcatcgaca ggcacagcgc cagacagagc agccagctga
     2161 tcacacgttt tttcatagat gtctcctttt atcctttaat caaacgccct tcgggcgatt
     2221 cctacatatc cttcggcacc agccacagct tctgcgccgc ggttctggcc tcgctgcggc
     2281 ggtgaaatgt cccaccagat caggcggctt ttcgcgtgct tgcgcaccgc gtttgtgcag
     2341 cttgtatgca aataagtgca gaaactccac ttatcttcgt gagaattata acagcttttt
     2401 gactattgcg taaatcagca caaagtatga aaatcatggt gctattttgt tgatatttcc
     2461 aaactagctg attatgtgcc gccggatttt caggcctttc cgcaaaaacc gtgcattttt
     2521 tcgaaccctc attgctggca gtatcttatg ctttaggctt tttcgaggtg cgccggtgca
     2581 gaagacgtat gaccgtttcc tctcaagata acgccatatt ataatatttt ctttgtgtat
     2641 tgtgtagcaa ccggcaccat tcgcagaaga atgcatcaca atgcctctcg acgagcaaac
     2701 agtttcctgc tatactgaaa aaagaaatat ctcaggagag cgagtgcgat gaagaagatg
     2761 ctctttgtgt gcttgggcaa tatttgccac agcccgatgg cggaattcgt gatgaaggac
     2821 ttggcaggat gtgttggcgg gatgccaagg acttctgaga gaattattgc aggagcgagg
     2881 taaacagaat ggataacgag cttcaaacga actatacaac agagcagacc tatcatt
//
LOCUS       DAWDRC010000509         2934 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12928_length_2934_cov_2.350122, whole genome shotgun sequence.
ACCESSION   DAWDRC010000509 DAWDRC010000000
VERSION     DAWDRC010000509.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12928_length_2934_cov_2.350122"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    805..814
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          742 a          756 c          709 g          717 t
ORIGIN      
        1 ttgctcctgt taacccgcta tcattaccgt tttcctccag cgggtttaac aggagtcctc
       61 gcatgaaata cattggagcg cacgttagtg ctgctggcgg tctggcaaat gccgcaattc
      121 gcgccgccga aatcgacgca accgcgtttg ccttgttcac caaaaaccaa cgtcagtggc
      181 gtgccgcacc gctcacgacg caaaccatcg atgaattcaa agccgcctgt gaaaaatatc
      241 actacacatc ggcgcaaatt cttccccacg acagttatct gattaacctc ggacatccgg
      301 ttgctgaagc tctggaaaaa tcgcgcgatg cctttataga tgaaatgcag cgttgcgaac
      361 agctggggct ttctttgctc aacttccacc ctggcagcca tctgatgcag atttcagaag
      421 aggattgcct tgcgcgtatt gccgaatcca tcaacattgc gctggataaa actcaaggtg
      481 tcaccgcggt gattgaaaac accgccggtc agggcagtaa cttagggttt aaattcgaac
      541 atctcgcagc gattatcgac ggcgtagaag ataaatcccg cgtcggcgtc tgcattgata
      601 cctgccatgc tttcgctgcc gggtatgatt tgcgtactcc tgccgaatgc gagaaaacgt
      661 tcgctgattt tgcccgcatt gtaggcttta agtatctgcg cgggatgcac ctcaacgatg
      721 cgaaaagcac ctttggcagc cgcgttgacc gtcatcatag cctcggcgaa ggcaatatcg
      781 gtcatgatgc gttccgctgg atcannnnnn nnnnatgcag gacgaccgtt tcgacggcat
      841 tccgctgatc cttgaaacca tcaacccgga tatctgggca gaagagatcg cctggctgaa
      901 agcgcaacaa actgaaaaag cggtggcctg atcgatgaac gacagggaaa agcagattct
      961 taaaatttta cggcgtaacc cgctgattca acagaatgaa atcgccgata ttctgcaaat
     1021 cagccgctca cgtgttgccg ctcatattat ggatttgatg cgcaagggtt tgattaaagg
     1081 caaaggatat atccttaccg agcaggatta ctgtgtggtt gtgggtgcaa tcaatatgga
     1141 tattcgcgga atggcggata tccgttaccc acatgcagcc tctcatccag gttctgttca
     1201 ctgttccgca ggcggcgtcg gacgtaatat cgcgcataac ctggctctgc ttggtcggga
     1261 tgtgcattta atatcagcta ttggcaacga tttttacggc gagacattac tggaagaaac
     1321 gcgccgcgcg ggcgtgaacg tttcaaactg cattcgtctg catggacaca gcactgcaac
     1381 ttatctcgct atcgctaata agcaggaaga aactatcctg gcaatcaatg acacacatat
     1441 tctgcaacag ctaacgcctc aactgctcaa tacttcacgg gacttgattc gccatgctgg
     1501 tgtggtgctg gctgattgta atctcacacc tgaagccctg gagtgggtct ttaccattgc
     1561 tgatgaaatt ccgatgtttg tcgataccgt ttccgaattt aaagcgaaca aagtaaaaaa
     1621 ctggtacagc cggatccaca ctctgaaacc gacacaaaat gaactggaaa ttttgtgggg
     1681 gcaaccgatt aaagacgaca acgatcgcat cagagctgta aattcgctgc atcaacaagg
     1741 tgttaaacgc atttttgttt atctgaaaga tgaatctgta ttttgcagcg ataaggatgg
     1801 agaaaaattt ttgctgaccg cgccagcgca tacgacagta gacagttttg gtgctgacga
     1861 tggttttatg gcgggcctgg tatatagctt tcttgaagga tacagtttcc gcgacagcgc
     1921 ccgttttgcg atggcctgcg cggcaatttc acgcgccagc ggcagcttaa acaaccctac
     1981 cctgtctgcc gataacgcgc tttcattagt gccaatggtg taacaatgtt gccggattcg
     2041 acgcttgacc gcatcttatc cggctcataa attgcgcact acgctaaacc aataaagaat
     2101 cctgcaatag tagcactcat caggttactg acagcaaaaa cgcaatagcc agtaatacca
     2161 ccattcccag aacacttctc atgacatcca tagttattat ctcttcatgt cattttgggc
     2221 gacggctctc tgcccatcat aaatagccgg gcctgcatct tgcgatactg acccggcttg
     2281 ttatcaacac ttaatggttt tttatgtagc cgcgtacgca ctcttcgact aatccccaga
     2341 accagtcggt atcaatagtg atgccgactt tggtattggc gggcttgcca agaacaccca
     2401 gctcgtcgca gacggtacgc ccatagcaag ggccactgtt aacgtccact tcgacgtaca
     2461 tctcctgggt tttaatgcca tcagggttaa tcaaataacc gatgcaggtg gcgtcgtgca
     2521 ccgggccgcc agccctttcc atccgagcaa tcacatccgg ggtgcaaacg gtctggttgg
     2581 taagatcgag gcccatcatc actaatggaa cgccggaggt gaacaccacg cgtgcggcct
     2641 ccgggtcagc aaagatgttg aactcagcag atggggtgaa gttgccagta ccataagcgc
     2701 cgcccatcag aacaatttca cggattttcg gcaggatcgc gggttgcata cgcattgcca
     2761 ccgcgatgtt tgacaacgga ccaaccggca ccagagtgat atcgccatcg cttgccataa
     2821 gggtatcgat gatatatttc accgcatgag tgttttctgc ctggcgggtc agcggctcga
     2881 ataccgggcc atccagtccg gtttcaccgt gaatattatc ggcaacaatt tgtt
//
LOCUS       DAWDRC010000510         2920 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_12995_length_2920_cov_2.653054, whole genome shotgun sequence.
ACCESSION   DAWDRC010000510 DAWDRC010000000
VERSION     DAWDRC010000510.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12995_length_2920_cov_2.653054"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2314..2323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          688 a          733 c          664 g          825 t
ORIGIN      
        1 tagtacactt cctctgattg atggggtgga aatcggtact cttgttgaac tggcgcaatg
       61 gacgctgtca gccgataaag tgctcacatt ttaatgtttg agggggcgtc tgacgcccca
      121 ctatgaggaa agtttactca ggcgaatccc ggccgtaaca caccatccgg ataattctcc
      181 gccagcagtt tttttaccga taccagcaat tcattcagcc catcgtcctg cattcccagt
      241 ttgataagct cctgttccag tgaaaacagg tattgtgcat tggttcccag cggaccgctc
      301 gccgccgcaa tcaacggcgc tatgacctga gcgcgagtgt cagattcata ttctggatgt
      361 cgcgggtcca taataaacac aatggcgttt actgtgcgtc catcatcaag atcaagctga
      421 caccaggttg gcagataaca gccggtaatc atctcgcgct tccacagcag ggttagttcc
      481 tgctccagcg tctcttctgg cagtcgatag gcgacgcctg tggtgcgtcc gccctctttc
      541 agtgcaagca ttcgtcccgg ctggtgcgca gtcccgcgcc cggcggtcag gcgcaggcaa
      601 aacgcgcgat gccatccaac cagtgtaccg gtgcacgact cggtaaactc cagtgccgga
      661 ttccacatca acgatccata accgaagatc cacaccggtc cctcatcagg tcgacaagcc
      721 agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc ataagagtga ttcttcaatg
      781 gcaccaaatg ccgttttaca atcggcattc atcaagaaat cacgcgttat caccttacac
      841 ctccgccact gcatacttcc ctgcgacaac tttttggtgt cttccggaca tttttactgc
      901 tcattttgaa accactaacg ggacaatatg caatccacgg accacttgca aggcaacacc
      961 gcacgaatag ctttaactaa gctaccggtt ttacgatttt ttatgccatt tatcatcatc
     1021 ccctttggca tattcatgct tcacagcagc ccaggccact ttatgcgctg tttcttcgcg
     1081 actggcgtca tcacgccgat cttctttatc tttatattga tcccatgcgc tgttgaacgc
     1141 ttctttatag atatcctggg catgagacgg aagcacgtgc tttacgcttt ccggcagatc
     1201 gcttttcgtt ttatacggca tcgttgactc cttttacaaa gttgaacatt aagtgtggtc
     1261 aacaatatct actcttgcca aaacataaga attttcatgt cagactcttc ttaagaagtg
     1321 ctaaaacaaa cattgtctaa acgtattact tatttttatg cccaaatgag taaaaaatcg
     1381 acagcggggt aaaaattcgt aaaatgttac cgatatccag acactttctg ttatttttcc
     1441 tacctatttt ttacattttc gataaattca tctgcaaagg gataaccata atgtcaaatg
     1501 ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct tattttcccg gttctggcgc
     1561 tggtagtgct gttcctgtgg ggaagcagcc agacactacc agtggtcatt gccatcaatc
     1621 ttcttgcgct tattggtatt ttaagtagcg cctttagtgt tgtccgtcat gcggacgtat
     1681 tagcccatcg cctgggagaa ccttacggtt cgcttattct tagcctttca gtggttattc
     1741 ttgaagtcag tttgatttca gctttaatgg caaccggcga cgccgcgcca acgctaatgc
     1801 gtgatacgct ctattcaatc attatgattg ttacaggtgg gctggttggc ttttcattat
     1861 tgttgggcgg acgtaagttt gccacccaat atatgaatct gtttggtatc aagcagtatt
     1921 taattgcgct gttccccctg gcgataatcg tactggtatt tccaatggct ctgcctgcgg
     1981 cgaatttttc aacaggtcag gcgttactgg tagcattaat ttctgcggca atgtatggcg
     2041 tatttttgct gatccagacc aaaacacatc aaagtttgtt tgtctacgag cacgaagatg
     2101 acagtgatga tgacgacccg caccacggta aaccgtctgc ccatagcagc ctgtggcatg
     2161 ccatctggtt gattgtccat ctgattgccg ttattgcagt caccaaaatg aacgccagcc
     2221 cgctggagac attgctcgac agcatgaatg cgcctgtcgc ctttactggc ttcctggtgg
     2281 ctctgttgat tctgtcgccg gaaggtttag gtgnnnnnnn nnncgttaaa agcagtgttg
     2341 aataaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttggc aaccatttcg
     2401 cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt gcagtttgca
     2461 cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tactgtgcca tatctccttc
     2521 tccaccggac gaactaacgt gctcaatggc gcagcgcatc tggcactgtt tgccgcctat
     2581 ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc gggggagata
     2641 atataaattc ctgcttatta tatttcttca tactagctgg acaggttgtt aatcagaatt
     2701 gcaagcctgt accacaacct gcacgaaatg actgcaacag ctgttgaaaa accggaatgt
     2761 actctttatt gcaaacattt cagggatgaa gtgaagatga attgcgacgt agtatccaca
     2821 ccaagccggc gttatccggt gaggcgcaat gttgcggggg ctttatctct ggtggcattg
     2881 gttgctggag agagaaaacc cccgcacgtt gagaggtata
//
LOCUS       DAWDRC010000511         2911 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13039_length_2911_cov_2.168417, whole genome shotgun sequence.
ACCESSION   DAWDRC010000511 DAWDRC010000000
VERSION     DAWDRC010000511.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13039_length_2911_cov_2.168417"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          709 a          751 c          781 g          670 t
ORIGIN      
        1 ccatcagatc caccggatgc tccgacatcg gaatcaaatg tgcgtccact ttcagcagat
       61 tgcgaatcag attgatcgct tcaagaggtc gcacgctaag gtgatccagt gccttcagca
      121 tcaggtttcc aaggttgtgt ccggaaagtt cgccattacc gccgaaacga tattcaaaca
      181 ttgcggaagc gacgctgggt tcagttatca gctggttgag gcagttacgc atatcgcccc
      241 aggcaatacc gccttctgag cggcgaatac gccccgtcga gccaccgtta tcggtggtgg
      301 tgacgatacc cgttaaacga gaacccaaag acgaaagtga tgagagaacg cgtcccagtc
      361 catgccctcc gccgagagca acgacacgat caagatcagc cagcgtacga ttgcgcatat
      421 agtttcctgg agtcagatta tccgcgctac agtagcgcaa attgcgggga aacagcaatt
      481 aacctgccaa tattgaggat acaaactatt ttcttctacc tctaaaggac gatgcacgct
      541 atgccttcct gatgatgtat atcaaagtaa aaccgccatt ttcccttatt ctgtagcgaa
      601 atagcacgat catgacgcta tatacatgat tgcatagcga aagtgtggat ggtaaaaatc
      661 tcatttacac gctagtatcg gcttaaccac taaacactct agcctctgca cctgggtcaa
      721 ctgatacggt gctttggccg tgacaatgct cgtaaagatt gccaccaggg cgaagaaaga
      781 aatgacttcg cctcccgtat ctggaaaggt gtacatggct tcacaactga ctgatgcatt
      841 tgcgcgtaag ttttactact tgcgcctgtc gattaccgat gtgtgtaatt ttcgttgcac
      901 ctactgcctg ccggatggtt acaagccgag cggcgtcacc aataaaggct tcctgaccgt
      961 cgatgaaatt cgccgggtta cgcgcgcctt cgccagtctg ggcaccgaaa aagtccgtct
     1021 gacgggcggt gagccatctt tacgccgcga ctttaccgat atcatcgccg ctgtgcggga
     1081 aaacgacgct atccgccaga ttgcggtcac caccaatggt taccgtctgg aacgcgatgt
     1141 ggcgaactgg cgcgatgcag ggctgactgg gattaacgtc agtgttgata gtctcgacgc
     1201 tcgccagttt catgccatta ccgggcagga taaattcaac caggtcatgg cagggatcga
     1261 tgctgcattt gaggccggtt ttgagaaggt caaagtcaat accgtgctga tgcgcgatgt
     1321 taatcatcat cagctcgaca cctttctgaa ctggatccag catcgtccca tccagctgcg
     1381 tttcatcgaa ctgatggaaa cgggcgaggg cagcgagctc ttccgtaagc atcacatctc
     1441 tggtcaggtt ctgcgtgacg agctactgcg tcgcggctgg atccaccaat tacgtcaacg
     1501 cagcgacggt cccgcgcaag tcttttgtca tccggattac gccggagaga ttggccttat
     1561 catgccgtat gaaaaagact tctgcgccac ttgcaaccgc ctgcgcgttt cctccattgg
     1621 taaactccat ctctgcctgt ttggtgaagg cggcgttaac ctgcgcgatc tgctggaaga
     1681 cgatatccag caacaggcgc tggaagcgcg tatttcagcg gcgctgcggg agaagaaaca
     1741 gacccatttc ctgcatcaaa ataacaccgg tattacgcaa aacttatcgt acattggtgg
     1801 ctaaaacgtc aaaaggagag atcagatgag tcaggtaagc actgaattta tcccgacccg
     1861 tattgctatt ctaacggttt ctaatcgtcg cggtgaagaa gacgatacct ccggtcacta
     1921 tctgcgcgac tcggcgcaag aagcgggcca tcacgttgtc gataaagcca ttgtgaaaga
     1981 aaaccgctac gctattcgcg ctcaggtatc tgcgtggatc gccagcgacg atgtacaagt
     2041 ggtgttgatt acgggtggta ctggcctgac ggaaggagat caggctcccg aagcgctgct
     2101 gccgttgttc gaccgtgaag ttgaaggttt tggtgaagtg ttccggatgt tgtcgttcga
     2161 agagattggc acttccacgt tgcaatctcg tgcggtagcg ggcgtcgcta acaaaacgct
     2221 gattttcgcc atgccgggtt cgaccaaagc gtgccgtacc gcatgggaaa atatcattgc
     2281 gccgcagctg gatgcccgta cgcgtccgtg taattttcat ccacatttga agaaataagt
     2341 aagtcgcaac tgacccatat caacgccgct ggcgaagcgc acatggtgga tgtctccgcc
     2401 aaagcggaaa ccgtgcgtga agcgcgcgcc gaagcctttg tcaccatgcg cagcgagacg
     2461 ctggcgatga ttattgatgg tcgccaccac aaaggcgacg tatttgccac tgcgcgtatc
     2521 gccggtattc aggcggcaaa acgcacctgg gatctgatcc cgctgtgtca cccgctgatg
     2581 ctcagcaaag ttgaagtcaa tttacaggcc gagccggagc acaatcgggt acgtatagaa
     2641 accttatgcc gcctgaccgg gaaaaccggt gtcgaaatgg aagcgttaac cgcggcctcc
     2701 gtggcggcgc tgaccattta tgacatgtgc aaagcggtgc aaaaagatat ggtgattggt
     2761 ccggtacgtt tgctggcaaa gagcggcggc aagtcgggtg actttaaggt ggaagcggat
     2821 gattaacgtt ctttttttcg cccaggtgcg cgagttggtg ggaacagatg caaccgaagt
     2881 ggctgcggat ttcccaaccg ttgaagcgtt a
//
LOCUS       DAWDRC010000512         2903 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13081_length_2903_cov_2.640098, whole genome shotgun sequence.
ACCESSION   DAWDRC010000512 DAWDRC010000000
VERSION     DAWDRC010000512.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13081_length_2903_cov_2.640098"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    287..296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1210..1219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          807 a          532 c          701 g          843 t
ORIGIN      
        1 gtacagttct tatatctatt aataataaaa agggatctac aacctacagg ttggtgtagc
       61 tttatggaaa aagactattt gggaattagt agtactgtat tagtgagctt attgtttggg
      121 cttgcattgg ttttggttaa tagttggttc aatcagccaa ccgttgagga agttgttcca
      181 cgatcaacct atctgatggt tatgattgct ttatttttta tcgatactgt tgcatttatt
      241 tttatgcagt tgtatttcat ttatgaccgt aggcaatttt caaactnnnn nnnnnntttg
      301 gcttttctaa gttgtttgat ttactttatt aaaaccgtca ttatcattca gcaaattatt
      361 gaggggcgtt tgacaagcag tgttgttcaa aatgacattg caatctatta tttgtttcgt
      421 cagatgagtt tgtgcatatt aatatttctg gcattggtga ataaagttag tgaaaacaca
      481 aaacagcgca atttattttc caaaaaaatg actttgtgca taagtttgtt ttttgttgtt
      541 ggggggccga tagttgctca catactttct agtcattatg agagctacaa tttacacatt
      601 gccgagttaa ccaatgaaaa tgatcaggtg gtttggaaaa catcatatgt taccataatg
      661 attttcatgt ggttaacctt gctttcagtt aatctatact ttaatggttt acgctgtgat
      721 atctggaatg gagtgacggt gattgctttt tgtgccgtac tgtacaatgt ctctctttta
      781 tttatgagca ggtatagcgt gtccatttgg tatattagtc gcactattga agtagtcagt
      841 aagttaaccg ttatggtaat atttatgtgc catatattca gtgcgctacg agtaacaaag
      901 gacattgcac atcgcgattc cttaaccaat atatttaaca gaaattattt ttttaatgaa
      961 ctgacagttc aatcagcatc ggccaaaaaa acgccttatt gcgtcatgat tatggatatc
     1021 gaccacttca aaaaagtcaa cgacacctgg gggcatccgg ttggcgatca ggtgataaaa
     1081 acagtggtga gtatcattgg gaaaagcata cggccagatg acctttttgc gcgcgtcggc
     1141 ggcgaagagt ttggcgtctt gctgacggac atcgatactg aacgtgcgaa agctttagcg
     1201 gaaaggattn nnnnnnnnng ataatcctga atacgccata ccacaaaaag tgacgattag
     1261 tattggcgct gttgtaacgc agaaaaatga gttaaaccct aaggagatct atcaactggc
     1321 tgataacgca ctttacgagg cgaaagagac cgggcgtaat aaggtggtag taaaagaggc
     1381 agaaattctt atcaaccgca aggatgatga atgagtctgt cgctagttgt cgacctccgc
     1441 ctggggcttc tcatcccccc ggtctatgca atatacgaaa aaaaagctcg tactttcgta
     1501 cgagctcttc tttaaatatg gcggtgaggg ggaccgcctg aaataaatct gacttaatcc
     1561 aggggggtta tataatttca ttataaacaa tgtattacac tttttgttgt ccgatattat
     1621 ctaatgtatt cttagtgaag cgagagacta atggcatact ggatggcata ccagagtgac
     1681 gggaattagc gtatggcagt attgacggat acgaaagcaa gacatatcaa acctgatgac
     1741 aaaccattgc cccatggggg aattaccgga ctgacccttc atccttcttc agtaaagggg
     1801 cgggggaaat gggtttttcg ttatgtaagt ccggtgacac aaaaaagacg taatgctgga
     1861 ttgggaactt acccagaggt cagtattgct gaagctgcac gtactgcccg gataatgcga
     1921 gagcaacttg ctgcaggtga tgatcctctg gagattaaaa aggctgaatc tgagaaagtc
     1981 gttatcccaa catttgccga tgcagccagg cgtgtacatg cagaactgtc tcctggatgg
     2041 gaaaatccaa agcatgtaag gcagtggtta tcgacgcttg agaattacgc gtttcctcaa
     2101 ctgggagcaa aaacgctgga ttcgattacg gctgcggacg tggcagaaac actgcgtcca
     2161 gtctggttaa ccttgtcaga aacggcaagc cgggttaaac agcgcattca tgttgttatg
     2221 cagtggggat gggcgcacgg tttttgtgtg gcaaatcctg ttgatgtggt tgaccatttg
     2281 cttcctcagc agacaagagg acgtgatgaa caccaacccg caatgccctg gaggcagtta
     2341 ccgctttttg tggcgaccag tgtgtatagt gatgaacctt ataatgttac ccgcgcactg
     2401 ttattaatgg tgatacttac agcaactcgc tcgggcgaag ccaggggaat gcgctgggct
     2461 gaaattgatt ttcataagcg ggtatggact atacctgcag aaagaatgaa agctaggcta
     2521 cagcatcgtg ttcctttatc ccggcaggct atttacattc tggaaaatat acgtggcctg
     2581 catgatgaac tggtgttccc ttcacccaga aagcagcaga tcctttccga tatggtgttg
     2641 acaagttttc tgcgtaaaaa gaaagccgtc agtgacattc cggggcgagt tgccacagca
     2701 catggttttc gctcaacatt cagggactgg tgtagtgaac aggggtattc gcgggatctg
     2761 gcggaaaggg cgctcgctca tacgctgaaa aataaggttg aggcggcata tcatcgtact
     2821 gatctactgg agcagcgtgt accgatgatg caggcatggg cggattatgt gatgtctcaa
     2881 attgtgaata aataatagtg aaa
//
LOCUS       DAWDRC010000513         2899 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13103_length_2899_cov_2.250352, whole genome shotgun sequence.
ACCESSION   DAWDRC010000513 DAWDRC010000000
VERSION     DAWDRC010000513.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13103_length_2899_cov_2.250352"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    431..440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2444..2473
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          732 a          708 c          743 g          676 t
ORIGIN      
        1 tgctccgaac gataagcatt tttaaagttt tcgtagtaaa gaattgctaa cgtataggca
       61 aatgcgtccc ataacgaaga tgtttggcta tttttacttt gcggcaaaat cacacttagc
      121 tcattaaata aggttttaat gacttctttt ccctgtcctg cggcaattac cgggcaagaa
      181 tgcggttgca gtagctgatt ttcctcagcc ccctgaaact gaaagccgta cagcgcacag
      241 gtacacgtcg ttgccggatc gttaacgtca gaggccaccg catgcaacct gccacgttct
      301 atcaccacaa tgtcatcggc atgcgcgaca tataacgaag aatcgatggt aaaccttgct
      361 acccctttct tgacgtaaat cagctcggtt tcattgtcat gaacgtgatg tccagactcc
      421 cattttggat nnnnnnnnnn ctaaatgcga aacgtgaaag ccgtggcgtt ttaccggcga
      481 caaacagcgt ttcactggca ttatcaaaac agcgttgata catgacaacc tccctattcc
      541 atgagcaaac aaaaacaata tatgccggat aaaatacggc gtgtattgcg gttatacaac
      601 cgcgtttagc gagagctaaa caagattatt tacactgaat gcaagattgc acggtcatgg
      661 aataactata ttgccgcaaa ttgcactttt gttttagcga tcgcattttt tttgcaagat
      721 tgttggcaag gaaaacagct tgctccgtcg aaaaccccgc accgctatcg cacactattt
      781 caggccattt ttaccttcca tcggagatgg ttccgtatgc gactcacagg agaaatcatg
      841 aaaataataa cctgctttaa gctggtgcct gaagaacagg acattgttgt cactccagaa
      901 tacaccctga atttcgacaa tgccgacgcc aaaatcagcc agttcgatct caatgccatt
      961 gaagctgcaa gccagcttgc aacagatgac gatgagatag ccgcgctgac cgtcggcggc
     1021 tctttgttgc agaactcgaa agtgcgcaaa gacgtgctat cccgcgggcc gcacagcctg
     1081 tatatggtgc aggatgcaca acttgagcat gcactgcctc tcgataccgc aaaggcgctg
     1141 gcgacagcag ttgaaaagat cggcttcgat ttactgatct ttggtgaagg ttccggcgac
     1201 ctttatgccc agcaggttgg cttgctggtc ggagaacttc tgcaacttcc ggtgattaat
     1261 gcagtgagtg ctattcagcg tcagggcaat acactggtga ttgaacgcac gcttgaagat
     1321 gatgttgaag ttattgaact ctctgttcca gccgtgctct gcgtcacctc cgatattaac
     1381 gtaccacgta ttccttcgat gaaagccatc ctcggcgcgg gtaaaaaacc ggtaaatcag
     1441 tggcaggcaa gtgatattgg ctggagccag agcgcaccac ttgctgaact gaccggtatt
     1501 cgcgtaccgc cgcaaacaaa acgtaagcac atcattctcg ataacgattc gccggaggcc
     1561 attgccgagc tggcggaaca tctgaaaaaa gccctgaact gaagcctacg gagaagaaac
     1621 gatgagtcaa ttaaacagcg tctgggtctt tagcgataat cctgaacgtt atgctgaact
     1681 gtttggcggc gctcagcaat ggggccaaca ggtgtatgcc attgtacaaa ataccgagca
     1741 ggcgcaggcg gttatgcctt atgggccaaa atgcatttat gttcttgagc aaaacgacgc
     1801 gctgcaacgt actgaaaatt acgccgaaag cattgctgcc ctgctgaaag ataaacaccc
     1861 cgctatgttg ctgttggccg cgacgaaacg tggtaaagct ctggccgcac ggttaagtgt
     1921 gcaactgaat gcggcgctgg tgaacgatgc cacggcggtg gatattgtcg atggtcacat
     1981 ttgcgccgaa caccggatgt atggcgggtt agcgttcgct caggaaaaga tcaacagccc
     2041 gctggcgatc attacccttg cacccggtgt tcaggagccg tgcacccgtg atatctctca
     2101 tcagtgcccg acagaaacgg taccttatgt tgccccgcgt catgaaattc tctgtcgcga
     2161 acgccgtgcg aaagcggcaa gcagcgtgga cctgagcaaa gcaaaacgtg tggttggcgt
     2221 cggtcgtggt ctggcggcgc aggatgacct gaaaatggtc cacgaactgg cggcagtgct
     2281 gaatgctgaa gtcggctgtt cacgtccaat tgccgaaggc gagaactgga tggagcgtga
     2341 acgttatatc ggtgtctccg gcgtgttgct gaaatccgat ctctacctga cgctggggat
     2401 ctccgggcag atccagcata tggttggcgg caacggcgca aaannnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnncgccggg agaacgcaag gtcacacgcg agaaaatctc cttcttaacc
     2521 gaagaaagcg ccgttaccct cgattttcac cgcgagcaac cagatgttcc gcaacacgcg
     2581 tcttataccg tattgcgtaa tcgtctggac ccgtggttga tggaacaagc cgagcaggcg
     2641 ggcgcgcagt ttatcccggg cgtacgcgtc gatgcgctgg ttcgtgaagg aaacaaggtc
     2701 actggcgtcc aggccgggga tgatattctc gaagcgaatg tggtgattct ggctgatggc
     2761 gttaactcga tgcttggccg ctcgctggga atggtttccg cttccaatcc gcatcattac
     2821 gctgttggtg ttaaagaggt tataggtctg acaccagaac agatcaacga tcgctttaat
     2881 gttacgggcg aggaaggtg
//
LOCUS       DAWDRC010000514         2898 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13107_length_2898_cov_2.553992, whole genome shotgun sequence.
ACCESSION   DAWDRC010000514 DAWDRC010000000
VERSION     DAWDRC010000514.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13107_length_2898_cov_2.553992"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          791 a          740 c          680 g          687 t
ORIGIN      
        1 caaacagcgg ttcaaacgca ggcacatccg tctgcgtttc tcgtccacgc ggggaaagca
       61 caccgttttc tgccgataag cgattttcag ctatgtactt cagctctttc atcacatcag
      121 aggtgcgttg aataaagcgc tgagcctgat cggaactcag attaaattcc tgacgaattt
      181 ccgattctcg ctgatgtaac gcattaacga tgtaaaaaac ggatgaaaaa gcaatcaaca
      241 gccagagcac taacgccaat gctctgaaca tgtagcgcga ggctttcagg gttgtacgaa
      301 aagaagcaag gtatttcaaa ggggcgaagc tccgcctcag gtgaccaatg gagtgtggtt
      361 aaggtagcgg taaaagcgtg ttaccgcaat gttctctctt ctctggaata tgatacaccg
      421 ccgagaaatc atcaccttaa cctctgataa tcgtcatata ccggacaaga ctagtggatt
      481 tcagcatgca ttatatgaag tggatttatc cacgccgctt acgcaatcaa atgatcctga
      541 tggcaatcct gatggtcatt gtcccaacgc ttactattgg ttatatcgta gaaacggaag
      601 gacgttcagc agtcttatct gaaaaagaga aaaaactttc tgccgtggtc aacctgctta
      661 atcaggcgct aggcaatcgc tacgatctct acatcgactt accgcgtgag gagcgtatcc
      721 gcgcattaaa tgcagaactt gcccccatta ccgaaaatat cactcacgcc ttccctggca
      781 tcggcgctgg ttattacaac aaaacgctgg atgcgataat cacctacgcg ccttcagcgc
      841 tatatcagaa taatgtgggc gttaccattg ccgcagatca ccctggtcgc gaagtcatgc
      901 gtacaaatac ccctttggtt tattcaggca ggcaggtgcg cggcgatatt ttgaattcaa
      961 tgattcccat tgagcgtaat ggtgaaatcc tcggctatat ctgggccaat gaattaaccg
     1021 aagatattcg ccgccaggcc tggaaaatgg atgtgaggat tatcattgtg ctcaccgccg
     1081 gtttgctgat aagcctgctg ttgattgtcc ttttctcccg tcgcctgagc gccaatatag
     1141 atatcatcac cgatggcctc tcgactctgg cacaaaatat tcccactcga ttaccacaat
     1201 tgcccggtga aatggggcaa atcagtcaga gtgttaacaa cctcgcccag gcactgcgtg
     1261 aaacgcgaac acttaacgat ctgattattg aaaacgctgc cgatggcgtc attgccattg
     1321 accgccaggg tgatgtaacc accatgaacc cagcagcaga agtcatcact ggctatcaac
     1381 gtcatgaact ggtagggcag ccttactcca tgttgttcga caatactcag ttctacagtc
     1441 cagtactgga tacgctggaa catggcaccg aacatgtagc actggagatc agttttccag
     1501 gccgtgaccg caccattgaa ctcagtgtca cgacaagtcg tattcataac acgcacggtg
     1561 aaatgatagg tgctttggtg attttctctg atttaactgc ccgcaaagaa acccagcgcc
     1621 gcatggcgca agcagaacgc ctcgccacac tgggtgagct gatggctggc gtggcgcatg
     1681 aagtacgtaa tccgctaaca gctattcgtg gttatgtaca gatcttgcac caacaaaccc
     1741 gtgacccaat acatcaggaa tatctgtccg tagtactcaa agaaatcgat tcaattaaca
     1801 aagttattca gcaattgctc gaattttcac gtccacgcca cagtcaatgg caacaagtca
     1861 gcctcaatgc attggttgaa gaaactctgg tactggtaca aaccgccggc gtacaagcgc
     1921 gggtcgactt cataagcgaa ctggataatg aattaagccc gattaacgcc gatcgtgaac
     1981 tgctcaaaca ggtactactg aatatcctga tcaatgccgt ccaggctatc agtgcacgag
     2041 ggaaaattcg cattcgaacc tggcagtata gcgactcgca acaggccatt tcgatagagg
     2101 acaacggcag tggcattgat ctctcgctgc aaaaaaagat cttcgatccc tttttcacta
     2161 ccaaagcctc aggaaccggg cttggtctgg cgttaagtca acgcatcatt aatgcccatc
     2221 agggtgatat tcgcgtcgcc agtttgccgg gctacggcgc aaccttcacg cttattttac
     2281 cgatcaaccc gcagggaaat cagactgtat gactgctatt aatcgcatcc ttattgtgga
     2341 tgatgaagat aatgttcgcc gtatgctgag caccgctttt gcactacaag gattcgaaac
     2401 acattgtgcg aacaacgggc gcacagcatt acacctgttt gccgatattc accctgacgt
     2461 ggtgttgatg gatatccgca tgccagagat ggacggcatc aaggcactaa aggagatgcg
     2521 cagccatgag acccggacac ccgttattct gatgacggcc tatgctgaag tggaaaccgc
     2581 cgtcgaagca ctacgctgcg gagccttcga ctatgttatc aaaccgtttg atctcgatga
     2641 gttgaattta atcgttcagc gcgctttaca actccagtca atgaaaaaag agatccgtca
     2701 tctgcaccag gcactgagca ccagctggca atgggggcac attctcacca acagcccggc
     2761 gatgatggac atctgcaaag acaccgccaa aattgccctt tctcaggcca gcgtcttgat
     2821 tagcggtgaa agcggcaccg ggaaagagtt gattgccaga gcgattcact acaattcgcg
     2881 gcgggcaaag gggccgtt
//
LOCUS       DAWDRC010000515         2894 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13131_length_2894_cov_2.112011, whole genome shotgun sequence.
ACCESSION   DAWDRC010000515 DAWDRC010000000
VERSION     DAWDRC010000515.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13131_length_2894_cov_2.112011"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1192..1201
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          781 a          656 c          666 g          781 t
ORIGIN      
        1 gtaaaagaag atataaatta agctattctt tatcttcaat aagccgcaac cccgcggtga
       61 tcacccctga caggagtaaa caatgtcaaa gcaacagatc ggcgtcgtcg gtatggcagt
      121 tatgggacgc aaccttgcgc tcaacatcga aagtcgtggt tataccgtct ctattttcaa
      181 ccgctcccgc gaaaagacgg aagaagtgat tgctgaaaat ccaggcaaga aactggctcc
      241 ttactatacg gtgaaagagt ttgttgaatc tctggaaacg cctcgtcgca tcctgttaat
      301 ggtgaaagca ggtgcaggca cggatgctgc gattgattcc ctgaaaccat acctcgataa
      361 aggtgacatc atcattgatg gtggtaatac cttcttccag gacaccattc gtcgtaatcg
      421 tgagctttct gcagagggct ttaacttcat cggtaccggt gtttccggcg gtgaagaggg
      481 cgcgctgaaa ggtccttcta ttatgcctgg cgggcagaaa gaggcttatg agctggttgc
      541 gcctatcctg actaaaatcg ctgccgtggc agaagacggc gagccatgcg ttacttatat
      601 tggtgccgac ggcgcaggcc attatgtgaa gatggttcac aatggtattg aatacggaga
      661 tatgcagctc attgctgaag cgtattctct gctcaaaggt ggcctgaatc tctctaatga
      721 agaactggcc cagactttca ccgagtggaa taacggtgaa cttagcagct acctgatcga
      781 catcactaaa gatatcttca ccaaaaaaga tgaagacggt aactacctgg ttgatgtgat
      841 tctggatgaa gcagcaaaca aaggcaccgg taaatggacc agccagagcg cgctggatct
      901 cggcgaaccg ctgtcgctga taaccgagtc tgtgtttgca cgttatatct cttctctgaa
      961 agatcagcgc gttgccgcgt ctaaagttct ctctggcccg caagcgcagc cagcaggcga
     1021 caaaggtgag ttcatcgaaa aagttcgccg tgcgttgtat ctgggcaaaa tcgtttctta
     1081 cgctcagggc ttctctcagc tgcgtgctgc gtctgaagaa tacaactggg atctgaacta
     1141 cggcgaaatc gcgaagattt tccgtgctgg ctgcatcatc cgtgcgcagt tnnnnnnnnn
     1201 ntacttcaag caaattgccg atgactacca gcaggcgctg cgcgatgtcg tcgcttatgc
     1261 agtacagaac ggtatcccgg ttccgacctt cgccgctgcg gttgcctatt atgacagcta
     1321 ccgtgccgct gttctgcctg cgaacctgat ccaggcacag cgtgactatt tcggggcgca
     1381 tacttataag cgcattgata aagaaggtgt gttccatact gaatggctgg attaatctga
     1441 tttaaccaac aataaaattg aggcccggcg tatattgcac cgggcttttt tttgccaaat
     1501 atctatagac taaatttcac tgcttaatat taacttaata aacatcagct attcttataa
     1561 agaaaatctg aattgttttt cgttgcgttg accatctgac aacgtagcgt taaaactttt
     1621 agcatcttat cacgatgcta aaaacatcat gattcacagt taagttaatt ctgagagcat
     1681 gaaatgaaaa tcaccatttc cggtactggc tatgtaggct tgtcaaacgg gcttctaatc
     1741 gcacaaaatc atgaggttgt ggcattagat attttaccgt cacgcgttgc tatgctgaat
     1801 gatcggatat ctcctattgt tgataaggaa attcagcagt ttttgcaatc agataaaata
     1861 cactttaatg ccacattaga taaaaatgaa gcctaccggg atgctgatta tgtcatcatc
     1921 gccactccaa ccgactatga tcctaaaact aattatttca atacatccag tgtagaatca
     1981 gtaattaaag acgtagttga gataaatcct tatgcggtta tggtcatcaa atcaacggtt
     2041 cccgttggtt ttactgcagc gatgcataag aaatatcgta ctgaaaatat tattttctct
     2101 ccggaattcc tccgcgaagg taaagccctt tacgataatc tccatccgtc acgtattgtc
     2161 atcggtgagc gttcagaacg cgcagaacgt tttgctgcgt tgttacagga aggcgcgatt
     2221 aagcaaaata tcccgaccct gtttaccgac tccactgaag cagaagcgat taaactgttt
     2281 gctaatacct atctggcgat gcgcgtagca tactttaatg aactggatag ctatgcagaa
     2341 agtttaggtc tgaatactcg ccagattatc gaaggcgttt gtctcgatcc gcgtattggc
     2401 aaccattaca acaatccgtc gtttggttat ggtggttatt gtctgccgaa agataccaag
     2461 cagttactgg cgaactatca gtctgtgccg aataatctga tctcggcaat tgtcgatgct
     2521 aaccgcacgc gtaaagactt tattgccgat gccattttgt cacgcaaacc gcaagtggtg
     2581 ggtatttatc gtctgattat gaagagcggg tcagataact tccgcgcgtc ttccattcag
     2641 gggattatga accgtattaa ggcgaaaggc gttgaagtgc tcatctacga accggtgatg
     2701 aaagaagatt cattcttcaa ctctcgcctg gaacgtgatc tcgccacctt caaacaacaa
     2761 gccgacgtca ttatttccaa ccgtatggca gaagagctta aggatgtggc agataaagtc
     2821 tacacccgcg atctctttgg cagcgactaa catcctgtta tcagggcgat tttcgccctg
     2881 attgtcttat gttc
//
LOCUS       DAWDRC010000516         2894 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13128_length_2894_cov_2.837619, whole genome shotgun sequence.
ACCESSION   DAWDRC010000516 DAWDRC010000000
VERSION     DAWDRC010000516.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13128_length_2894_cov_2.837619"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          766 a          617 c          723 g          788 t
ORIGIN      
        1 tttcattaag tatttctaac aacttaatgt atatatttta aatgtctcca ggctatttca
       61 ccaggttatc atttgatgct aaccttctga cacatgcagt ggagttgttg tgcagcagga
      121 gtatgctgat atgaaagtga atgctaagga taatttattc gcttaatcta ttaatttgct
      181 ggaaatattt aagggatgaa tgccttgaat aaaccatcat gatttttaga cagtggatgt
      241 ggaggaaata tgttgccagg atgctgcaaa aatggaattg ttatcagtaa aatacctgtt
      301 atgcaagcag ggttaaaaga ggtcatgagg actcacttcc ctgaatatga aataatatcc
      361 agcgcctctg cggaggacct taccttatta caattacgtc gttccggatt agtcattgct
      421 gatttggccg gtgaaagtga agatccacgt tccgtttgtg agcattatta ctctttaatc
      481 tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta ttcccaggca
      541 gtagaattgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc cattgagaat
      601 ctggtcaaga ccgtacgctc cggcaatacg cactcagagc gtatcagcgc catgctgacc
      661 tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct cactctttca
      721 gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat agcttcattg
      781 cttaagaaaa gtaataaaac tattagcgcc caaaaaaaca gtgcgatgcg tcgactggca
      841 attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc aagagaactt
      901 aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt aagaagagaa
      961 atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt ttattcagaa
     1021 agtttgcggg taacccttat aagctccata cctataccag tcaggagtca tttcaggatg
     1081 ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg agaagtgcgc
     1141 gcagagaggg attatcctgc ctgactgaac tggcgattaa gtttccgcgt acccggcgtt
     1201 tagttattgc ggatgatgat attgaagctc ggctgattgg ttcattgtcg ccatcaccgc
     1261 tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa ctctttttgt
     1321 cattaaatgg tgtacgtcag gcgaccgatc gactgaacaa tcagtggtac attaaccaaa
     1381 gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt ggctactcaa
     1441 tgacacaaat tgccgagcag cttaaacgca atatcaaaac gatccgcgcg cataaattta
     1501 atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc gcagatattc
     1561 tgttatgtat gcggcattgc gaaacaagta atgtgttgca tccttattaa tccgcatgat
     1621 gccgggttta cttccccggc agtgctttca tttcagcgta caatcgccac attgctgcac
     1681 atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt cgcgtagcac
     1741 cacggtacgc cagagtggat tatcttcacc ggtagtaagc gttttctcaa aaaagagggc
     1801 atagcgcagc gattcaaccg tcgcctcgcc gagcagctgt ttcatctcag tcagatacca
     1861 gttgatcaaa taaccggtat tgctccagat aagtttgccg ttaatctctc ccgttgcttc
     1921 tagtgcctgc acaaccggaa ccagcgcctg gctgattaac gtttccattc gctgctgcgg
     1981 cgaatgtagt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg cggcgcgtcc
     2041 ggtttcgtga aactcaacgt ggacatgttc cggcgacaca tctaatgctt tttcctgagt
     2101 cagcagcgcc agcattaatg gtggtaccat caggccgata taccattgcg cccacagtga
     2161 gataagcggt ttgttttcgc ggatcatcaa aggttggttg cggtagatat gatcggaata
     2221 gaccgcgagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg tcatggcgtt
     2281 aagtggggcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat gttcggcgat
     2341 cgttgcccgc acggcttgtg caagcccagc atcctgcggc tggagatgcg ttcgccagat
     2401 aacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa tctaaatgat
     2461 aattattgct aatcctatcg ataggtttac ccgatagcaa gggatttctc tggcttgcaa
     2521 atgataaaaa ttatcatatg atattagtta tcattatcaa tgaaagagat gaaaccatgt
     2581 tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg attgcagggc
     2641 taatgtatgc ggatttatcg ccggatcaat ggcggattgt cattctgatg ggattggtat
     2701 tgacgccggt gatgctgtat cacaaacagt tacggcatta cgttttgcta ccatcgtgcc
     2761 tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcagggg tgataaaaca
     2821 gtgaagagac aagaaaagaa gtaaagataa ttggtgcgag gggggggact tgaaccccca
     2881 cgtccgtaag gaca
//
LOCUS       DAWDRC010000517         2885 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13179_length_2885_cov_2.315901, whole genome shotgun sequence.
ACCESSION   DAWDRC010000517 DAWDRC010000000
VERSION     DAWDRC010000517.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13179_length_2885_cov_2.315901"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    365..374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1507..1516
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          670 a          723 c          751 g          721 t
ORIGIN      
        1 ccttccctgc tgattagccc ctatcccctt tcctctttgt tttccgacta cattcaccgg
       61 ataaatttta ttctccagtg ttatatacta taggagggta tgcattgaca tatagcatac
      121 ccccctatag tatattgcgt gcagatgatg aggtgcgaaa tgcccagtac tccggaagag
      181 aagaaaaagg tccttactcg agttcgtcgt attcgggggc agattgatgc tctggaacgg
      241 tcgctggagg gtgatgccga atgccgtgcc atactccaac agatcgctgc cgttcggggc
      301 gcggctaacg gactgatggc agaagtgctt gaaagccata tccgggaaac gtttgaccga
      361 aatgnnnnnn nnnncgcatt taccctctcc ggcgatgacc cggaaggtgt cttcccagtg
      421 gttctcggtc acgaaggggc cggggttgtt gttgaagtcg gtgaaggcgt aaccagcgtc
      481 aaacctggcg accatgtgat cccgctttac accgcagagt gcggcgagtg tgagttctgt
      541 cgttctggca aaaccaacct gtgtgtcgcg gttcgcgaaa cgcagggcaa aggcttgatg
      601 ccagacggca ccacccgttt ttcttacaac gggcagccgc tttatcacta catgggatgc
      661 tcaacattca gtgaatacac cgtggtagcg gaagtgtctc tggccaaaat taatccagaa
      721 gcaaaccatg aacacgtctg cctgctgggc tgtggcgtga ccaccggtat tggcgcggta
      781 cacaacacag ctaaagtcca gccaggtgat tctgttgccg tgtttggtct tggcgcgatt
      841 ggtctggcag tggttcaggg cgcgcgtcag gcgaaagcgg gacggattat cgctatcgat
      901 accaacccga agaaattcga actggcgcgt cgcttcggtg ccaccgactg cattaacccg
      961 aatgactacg acaaaccaat taaagatgtt ctgctggata tcaacaaatg gggtatcgac
     1021 catacctttg aatgcatcgg taatgtcaac gtgatgcgtg cagcgctgga aagcgcgcac
     1081 cgcggctggg gtcagtcggt gatcatcggg gtagcaggct ccggtcagga aatctccacc
     1141 cgtccattcc agttggtcac cggtcgcgta tggaaaggtt ccgcgtttgg cggcgtgaaa
     1201 ggtcgttccc agttaccggg tatggttgaa gatgcgatga aaggtgatat cgatctggaa
     1261 ccgtttgtca cgcataccat gagcctggat gaaattaatg acgccttcga cctgatgcat
     1321 gaaggtaaat ccattcgaac cgtaattcgt tactgatttc ccgctggttt accccgtcca
     1381 gttcagacgg ggttcttaat actctcccca ggcagccgtc cgggggatta accgtgagat
     1441 aatgactgat ggaactcatt gaaaaacatg ccagctttgg cggctggcaa aatgtgtatc
     1501 ggcattnnnn nnnnnnttgc cggtgttata ctggctttca ggcctgacct gcaacgagca
     1561 gaatttcatt actaaatcgg ggatgcagcg ttacgcggct gagcacaaca ttattgttgt
     1621 tgcgccggac accagtccgc gaggcagtca tgtcgcagat gctgaccgtt acgatctcgg
     1681 gcaaggagcc gggttttacc tgaacgcgac gcaagcgccg tggaatgaac attacaaaat
     1741 gtatgactat atccgcaacg agctgccgaa tttagtgatg catcattttc cagcaacggc
     1801 cagaaagtct atctcaggtc attctatggg cgggctgggc gcgctggtgc tggcgttacg
     1861 taacccagat gaatatgtca gcgtctcggc gttttcgccc attgtctccc catcgcaagt
     1921 accgtgggga cagcaagcct ttgccgcata tcttggtgaa aataaagatg cctggttgga
     1981 ttacgacccg gtgagtctta tttcacaagg tcaacgcgtt gcggaaatca tggttgatca
     2041 ggggttgagt gatgattttt acgcagaaca actgcggact ccaaatcttg aaaagatctg
     2101 ccaggagatg aatatcaaga cgttaatccg ttatcacgag ggttatgatc acagctatta
     2161 ttttgtctcc agttttattg gcgagcatat tgcgtaccac gccaataaac tgaatatgcg
     2221 ttgagcatcc gcgcacgcat gccggatgcg gcgtaaacgc cttatccggc ctacatttca
     2281 cccgtaagcc gtaggcctga taagacgcga aaagcgtcgc atcaggcatc tgcgcacggc
     2341 tgccggatgc ggcgtgaacg ccttatccgg cctacacttc gcccgtaaac cgtaggcctg
     2401 ataagacgcg taagcgtcgc atcaggcatc actgcacggc ttatctatgt ttaccacatc
     2461 aaatcatcag gcactttgaa gtcggcatac ggatcgtctt catcctgctc ttcggcgctg
     2521 agcgcgctgt gcaacacaat actgctggca tcacgctgag caattttatc ggcaacgctg
     2581 gcgggaataa tgacgtattc accttcgtta ttgttatcga ccaacaagcg ggcaatcgcc
     2641 agacgaccat taatcaactg cgtttgcgtg attttatcga caaagatttt cttaatcaga
     2701 ttgccgtcag tgaagttaaa accaatatcg ccattggcaa tggtaattcg gttcatttca
     2761 atgagctgct tcacctgggc tttatattct ttcgccaacg ccgcttgttt ttgttgctcg
     2821 ctaagctgtt tatcacgctc aagctgtgcc tttttatttt cttctaccgc cgcccgagct
     2881 tcacg
//
LOCUS       DAWDRC010000518         2882 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13196_length_2882_cov_2.807216, whole genome shotgun sequence.
ACCESSION   DAWDRC010000518 DAWDRC010000000
VERSION     DAWDRC010000518.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13196_length_2882_cov_2.807216"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    393..402
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          711 a          767 c          727 g          667 t
ORIGIN      
        1 gcaggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
       61 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
      121 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
      181 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
      241 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
      301 actgtgcaat tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
      361 gctgcgcgag aagttgggct gggatatcga acnnnnnnnn nnaacaaacg gcagccagag
      421 cgcgtttttc tacttattta atctgtttgc cggacgccgt gccgatggtc gggtcaaaaa
      481 agtgctgttc ccgctggcac cggaatacat tggctacgct gacgccggac tggaagaaga
      541 tctgtttgtc tctgcgcgtc cgaatattga actgctgccg gaaggccagt ttaaatacca
      601 cgtcgatttt gagcatctgc atattggcga agaaaccggg atgatttgcg tctcccggcc
      661 gacgaatcca acaggcaatg tgattactga cgaagagttg ctgaagcttg acgcgctggc
      721 gaatcaacac ggcattccgc tggtgattga taacgcttat ggcgtcccgt tcccgggtat
      781 catcttcagc gaagcgcgcc cgctatggaa tccgaatatc gtgctgtgca tgagtctttc
      841 caagctgggg ctacctggct cccgctgcgg cattatcatc gccaatgaaa aaatcatcac
      901 cgccatcacc aatatgaacg gcattatcag cctggcacct ggcggtattg gtccggcgat
      961 gatgtgtgaa atgattaagc gtaacgatct gctgcgcctg tctgaaacag tcatcaaacc
     1021 gttttactac cagcgtgttc aggaaactat cgccatcatt cgccgctatt taccggaaga
     1081 tcgctgcctg attcataaac cggaaggagc cattttcctc tggctatggt ttaaggattt
     1141 gcccattacg accgagcagc tctaccagcg cctgaaagca cgcggcgtgc tgatggtgcc
     1201 ggggcacaac ttcttcccgg gactggataa accgtggccg catacgcatc aatgtatgcg
     1261 catgaactac gtaccagagc cggagaaaat tgaggcgggt gtgaagattc tggcggaaga
     1321 gatagaaaga gcctgggctg aaagccacta aaaattgcct gatgcgctac gcttatcagg
     1381 cctacatgat ttctgcaata tattgaattt ggtagaattt gtaggccgga taaggcgttt
     1441 tagccgcatc cggcataaac aaagcgcact atccctgcgt tgagaaacgc cggaaaaacg
     1501 tttcaaacag gctgctgccg tagccgatgc cgctgcactt tctcgacatc catgcactgc
     1561 aacgcctttg tcgggcaggc ttcaacacag gccggccccg tcgcccgatg ccagcacaga
     1621 tcgcacttaa tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc accaaacggg
     1681 caagccagca tacagctttt acagccaatg caacgtgatt gttcaacgaa aatatgccca
     1741 tgttcgcggc ttatcgcgtc aacagggcac acatttgcgc acggtgcatc ttcacactga
     1801 tgacaggcta ctgccgtggt gtaagtatga tctttaatga cacgaatacg ggaaataaac
     1861 tcgtctggtg acaacgcagc gcaatcctga ttctcctgat gcgacactgc gcaagccact
     1921 tcacaggtac ggcaaccgat acatttcgtc gcatccgcaa taataaaccg gttcatgatc
     1981 actcctttgc actaaaacag cagagtgcca gagagcgcgt ccctactgct ttgatccaac
     2041 gaaggaaatg ttaaagtttt ggcacttgtc caaagggtta tgtaatcgcg ccctggtcgt
     2101 ccctgacagt aaatcccagt tcattagaaa tagcctgcgc ggtttcacga agtggtttca
     2161 ggagattttt ctctcccacc tgtttcagac gtgatgtcga aagcgaaatc gacacggcgt
     2221 acggcacccg cccatgaata tcaaacaccg gaacagcaat acaggagacg ccgagttcgt
     2281 tttcttctct gtccatcgcc gctccacttt cacgaatgtg cgccagttcg tcgaacatcg
     2341 cgggcagctc ggtaatggta ttgcgggtta acggctggat ctcatgctga tggctttccc
     2401 agtatgactt cacgtagtcc gggtgaccaa acgccatgta gatcttgccc attgcggaac
     2461 agtagagcgg catatgctgg cctatatagg cacgggttcg cagcatcccg gttgtgggtt
     2521 ccagcttata aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta atagtttcac
     2581 cagtggcgat gttcagagcc tcaagatgcg gagcggcgat atgaatgata ttcagcgaag
     2641 atagcgcctt ctgcccgacg gcaataaatt tggtggtcag gcgataactc cctgcggcgg
     2701 gcgcggtggt cacatagccg caggactgta atccctgcaa taagcgatgg acggtactct
     2761 tatttaaacc agccagctcc gaaagatgcg ccaacggaca accgtttgga tagttgctca
     2821 aaatctcaat cagcatcaac ccacgaaaca gactctggct tccggctggg cgctcttttt
     2881 cc
//
LOCUS       DAWDRC010000519         2882 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13198_length_2882_cov_2.489919, whole genome shotgun sequence.
ACCESSION   DAWDRC010000519 DAWDRC010000000
VERSION     DAWDRC010000519.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13198_length_2882_cov_2.489919"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          832 a          630 c          581 g          839 t
ORIGIN      
        1 acaactggcc tgtgatgcac tgcaaatggc gttgtggcgg agaagacgcc cggaaagcgt
       61 cattgttcat acggaccgtg gtggtcaata ctgttcaggg gattatcagg cgctgctgaa
      121 gcgacacaac ctgcgtggca gtatgagtgc gaaaggctac tgttatgaca atgcctgtgt
      181 ggaaagcttc tttcattcgc tgaaggtgga atatatccac ggggaacgct ttagcagccg
      241 ggaaataatg cgggcaacgg tgtttaatta tatcgagtgt gattacaatc gctggcgtcg
      301 tcacagtgcc tgtggcggtc tcagcccgga acaatttgaa aaccataatc tcgcttagga
      361 ccgtgtccac attacgtggg taggatcata ttacctgtat gaaactcaca tcccacggta
      421 cggatcacgg tcacgcctat caactcatgc tgggcagcga tgttaaattg cacaccattc
      481 cgatgagata ccctgactga ccttgaacaa aaggtttacg ctactttgta ttatgttgtt
      541 cactggtgaa tactccctta gcaaggtacc cataactcag ggaggtaaaa gtacaatcag
      601 ggctggcagc actgcatgtg ctctccgggg gtttaccaga acattttctc atttaacgca
      661 acattgtgaa cagcgaagac gtttctggca agaacgatac caatgatagt cataatgaat
      721 tcctcaagaa ctattgatga ctttagcatc atctccacag acatattata aaatatgatt
      781 tagcatgcat ccgattattg attagcgccc tatcgtgttc tttggaaatt aactttttat
      841 catagtctta attactcttt ttaagccaac agagacaaaa tagcaccgca accatgtcac
      901 agatgacacc tacgcaagta cagtatcatt tgccaagaag ggctagggaa tgaagcataa
      961 gagctaactg gtttgacaat atacatacac ataggcccca cagataatac cctggaagat
     1021 acatccattg gagatggtat attagaaata ctgcaactcc taataaaaat attaatgtca
     1081 gagtgataac acttctgcca ttcatgtcat attttatttc ccttccaaca atgaactgta
     1141 cagataaaac atctgtttct ttgatgccca gattgggtgt attaagataa ataatttcat
     1201 ttttccctta aaaaatgata ctgaatagtt gaaattaaag ttgtgctgcc agagttgata
     1261 ataattgttc tgtctgttgc catgaaagac actcatcagt aacagactga ccatactctc
     1321 gcggaaggct gtctgattgt tttccacctt gtaaaaagct ttccagcata attccagcta
     1381 catatcccgg catctttttc cgattatcaa tgacttcacg agcaacagag atttgccgtt
     1441 cagccacttt accactatta ccatggctac agtcaattat cagacgatga ttgatacctt
     1501 catcatgcat taatttcact gccttcgtta tatccgacaa accatagttc ggctctctac
     1561 caccacgtaa tatgagatgt ccatgtggat tgccatcggt taatagggta gatatacatt
     1621 ttgttaaaga tgtcatatat acaacatgtt gttcacgggc tgcaagaata gcatcaatag
     1681 ctaagttaat atttccatca gtgctgtttt taaatccaac aggacaatgt aaaccagacg
     1741 caagttgacg atgcgtttgt gattcagtag ttctggcacc aacagccccc caacatatta
     1801 aatcagcaat atagggagta aggaaaggat cgaggaattc tgtagcggtt gcgacacgca
     1861 ttgttgtgat tgaggacaaa cactggcgag catatcgtat ccctttctca acattatagc
     1921 ttccatttag atcaggatcg tgcattattc ctttccagcc tttacgagtt cgaggtttct
     1981 caaaataagt gcgcattaca atgtacattt gcgataagta cttattctgt aatacggaca
     2041 atcgtttcgc atattcaacg gcagcctgaa cgtcatggat tgagcatgga ccaacaatca
     2101 caagcaagcg aggatctttt cccagcaaaa tattcgctat aatctctcgt tgcagagaga
     2161 tccatgttac ggtttcttct gatactgcga tttctttatg aatctcgccc acagcaggta
     2221 aactgctaag caacttccct cggtagattg atttactgac tgactgcatt gtatctcctg
     2281 aaagttctat tcttaaccat ctattgcaca cactaattga taatgataat catttaatgc
     2341 atgtttgaca atacaggatg ttccgtaacc atttctgcga taactcacgt taagcctttt
     2401 caccttgaaa tcacttgttt tccgtaaggt cctacagttg aagaaaattg aatctataaa
     2461 tctgtgattt cttgcgtgac aaactcgcac gatacgcttt ttggattacc tgaactttaa
     2521 ttccccgatg agcactctcc tgcgaaaata ttgcatttgc cagagctcag cggcggtctc
     2581 ataaccgtct tggttttgcc attcagcttg cacttgtccg tgctctcggt cgcacactgc
     2641 gtacagaaaa gctccccccg aaggctgtta tcacaatagt ggcggaacaa ctgggtattg
     2701 accttgccgt tttaaagatt aatgtcacga acggtgcaaa agtggttctt ctgcaacctg
     2761 agtggaagaa aatggatgaa actttctggt gaacaaagat ctaagctgtt ttaagttttg
     2821 ggcaactcaa tgcactctct taagacactt ctacagttac cttgcggatg gcgatgcagt
     2881 cg
//
LOCUS       DAWDRC010000520         2882 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13197_length_2882_cov_2.704280, whole genome shotgun sequence.
ACCESSION   DAWDRC010000520 DAWDRC010000000
VERSION     DAWDRC010000520.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13197_length_2882_cov_2.704280"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1543..1552
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2168..2177
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          800 a          727 c          665 g          670 t
ORIGIN      
        1 agatcaaacc gattctggaa ttgatgggta aaaatatcac tctggtcggt aacgtcggtg
       61 atggtcaaac gtgcaaagtc gctaaccaaa ttattgttgc actgaatatt gaagcggtgg
      121 ctgaagccct gctctttgcc agtaaatcag gtgccgatcc cgcccgcgta cgtgaagcgc
      181 tgatgggagg gtttgcctcc tctcgcgtgc tggaagttca tggcgaacgg atgattaaag
      241 gaacattcga acccggtttt cgtattagtc tgcatcaaaa agatctgaat ctggcgctgg
      301 agaatgcccg cctgttgaat accccgcttc ctaacacggc gacgacacag cagttattta
      361 gcgcctgcgc cgcgctcggt ggtaaagagt gggatcattc tgcgttaatt cgggcgcttg
      421 aaacacaagc caatttcact atccgtaaat aatgaattaa gggaaccacc ggttcccttc
      481 gttcactcat tgccctgccc cattccccta ttttgagaga cctgctatga agatagttat
      541 ctcccccgac tcctttaaag aatgccttcc tgcatggaaa gtcgccgaag cgctggcaac
      601 cggctggcgc aaggtcctgc ctggcagcca actggtgtgt ttgcccgtgg ctgacggcgg
      661 cgaaggcacg ctcgaaacac tcattcatgc gactgacggt acgttttaca ctaaaaaagt
      721 caccggaccg cttggcgaat caatacatgc gcaatacggt attttaggca accaaaccac
      781 cgcagtgata gaactggcac aagcttcagg gctggaactg gtttctcctg tccagcgttc
      841 tcccctttat acgacgtcgt ttggcactgg cgaacttatt ctcgccgccc tggaacacaa
      901 tattgatacc gttattctat gcctgggtgg cagtgctaca aatgatggcg gtattgggtt
      961 gatgtcggca cttggcgcat cgtttacaga cgccgaaggg ctatcagtct ctgttaatgg
     1021 gatggggctg gcggcaattc accacattga cttacagcac ctcgatccac gattgaaaaa
     1081 tgtgaaattt attgcagcct gcgatgtcac caacccacta accggcgata acggcgcgac
     1141 ccgggttttc ggtcaacaaa aaggggccag tgctgacgac cttgagcaac tggaacaggg
     1201 aatgaaaaac tatgcccgtt gcatctacct ttgttgtggt aaagaagtcg atacgatacc
     1261 cggttctggg gcggctggcg gcgttggcgc ggccttgatg gcttttctcg atgctcgctt
     1321 acaaccgggt atttcgctcg tgctggaagc gattcaatat acccaacatt taaaatacgc
     1381 agcattggcc attgtcggtg aagggaaatt agatagtcag agcctgaatg gcaaagcgcc
     1441 cgtaggcgca gccaaagttg cccagatgat gggcgttccg gttatcgcaa ttgccgggta
     1501 tatcgatgat caacttgatc tgaatgagtt acgccagtgt ggnnnnnnnn nnagtgtgga
     1561 atagaagcct gtttttctgt cgtcaacggc ccttgcaatt tacccaccgc gctgagtcag
     1621 ggagaaaata atttaattcg tcttggagaa aatctggcgg gatattttcg tgcgatcctg
     1681 agttaattcc cgttcggtaa aatgcccgat aatgcaaatt accgggcatt cctgaacatc
     1741 attattcatc tctcgcgttt ttctcttcag ccgcaagctg tgtcaaaaaa ctcttcaata
     1801 aaataaacac cacgccgcaa agcggtagtc cagccagaat agacaccgtt tgtagcggtt
     1861 gcagactttg cgcatcaaga agatagagtg tgatggcgac gattccagtc ataatcaacc
     1921 aaaatgccac gttccagaat gccggttcac tctcactatc tgcccgtttt tgcgtcgcaa
     1981 aaccgagggc ataacccaca gtacagtgtc cggtaatata tgaaattaat gctatagccc
     2041 caaaagcaac caaaaacacc tgacttgcgg gtagctgctc cagtaaacga gaaacgacaa
     2101 cgcctcgccc tttttcgtta agtatgggaa caaaatcgat gacatgagtt gtctgcaacg
     2161 ataagccnnn nnnnnnngta ccatgtaaaa aaccatgcat cccaatgaac ctgccagagt
     2221 tccacataca atcaattgcc ggatcgtcct tccttttgaa atacgggcga taaataaacc
     2281 gaatgggatg gcatacacga cccaccatga aaaataaaat acggtccaca tctgtggaaa
     2341 tccgctacga ccaatagcat cggtacttaa actcatcgct acaaaatttt gcagcatcag
     2401 gccgagacca tcaattgatt gattaagtat aaatgccgtt ggtccacaaa tcagtattgc
     2461 cagtagcatc aaaatgtcag cacgaatatt ccagttactg gcccatgcca cacctttttt
     2521 aagtccaaaa aagagaatga ataatggtac tagcgtccag ataaaaataa ccatgacatt
     2581 gataaactgc ccggcaggat ccagatggaa cacatggcaa aatacggcag aaatcatcgg
     2641 cgttcccagc gccagagacg ttaccgcgcc gccgagcaaa ccaaacatgt agaaaaaatt
     2701 cacacaatgc ccgcaaaaac catccgcacg cgctcccaaa catccacgac ataaatcact
     2761 gacctttaat gagttcgttt tcttaacatg cataaaccaa ccaatgggaa cggcacaaat
     2821 cagataccac gcccatgcgg acggccccca atggaacatg ccataagcag aggcccaacg
     2881 ag
//
LOCUS       DAWDRC010000521         2873 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13240_length_2873_cov_1.623847, whole genome shotgun sequence.
ACCESSION   DAWDRC010000521 DAWDRC010000000
VERSION     DAWDRC010000521.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13240_length_2873_cov_1.623847"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          687 a          769 c          714 g          703 t
ORIGIN      
        1 ataatcctgc gccagttgca tcgccgcttc tgtagcctgc ttctctttct cctgcagata
       61 acccaccagg ctaatctgat acttacgaat attttcgaca taagcgtagg cttcatgccc
      121 acgagcgtag ccgtaagtca gcttgctgta atagggtttc tggctaagta aaggcagacg
      181 ctgttttacg tcagcccaac tgtcaggatt acctttggtt tttgccgtca gggcgcgggc
      241 atccagcata tgcgcatagc ccatattgta cgcggcgagg gcaaaccaga tccgttcgtt
      301 ctccggcaca ctttccggca ctttactcat catatcctgc aaataacgca cgccgccgct
      361 gatgctctgt tcggcatcgg tacgatccgt aatgccgagg ctttgcgcgg tgtttttggt
      421 taacatcatc atgccgcgca cacccgtcgg tgaagtggcc tgcgcatccc agtgcgattc
      481 ctgataagca atagcggcca gcaaacgcca gtcaatttct tcggcgtatt tctcaaacag
      541 gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa aatgtgcgcg tatcgacgta
      601 atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg cgtgccagcg taccgtcttc
      661 attcatctca ttgaagaaat cgagcagagc ggcggaaagg gtattatcgc catctaacgg
      721 gctaaaccag gtcaccggtt gttcatcggt gatatcgagc gctacggcga gttccgggtg
      781 aacacgctga aacaggctga tggcgacaga atcagcaatg gtgtaatcga gttttccttc
      841 gatgacatct tccattaatt ccgcagagcc ttttttgtcg tctaccttcc agcttaattc
      901 cgggaatttt gtgtctttca gggtctggag atcgttaacc accacgtgac ccggtgcaac
      961 ggtgagttgc tccgccgtca ggttgcccag cgtacgtggg cgatactgac ccactttata
     1021 aaccagttgt tgtgacacgg aataatagat aggaccaggc tgataatttt ttacccgctc
     1081 actgttatag accagtcctg ccgccagcag gtcggcgtta ccattatcga ggtcgtcaaa
     1141 cagctggctg atattctgcc gcacggtcac tttcagtttt acaccgaggt aatcggcaaa
     1201 ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg ttgatttcgt tatacgtcag
     1261 gggagtatga atggtgctca cacgcaactc tccccgcgct tgaatggcgg cgatacggtt
     1321 gtcggcttta ccaaaccagg gaatggatgg ccagagagcg accgcgagca gcagtgccag
     1381 aatgccgatg aacagataat taatctttaa ttttttcaat tagttaattc tctgtgtcgt
     1441 gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt ttactcataa atgagcggca
     1501 ttttgcgtaa acctgcgcca gatggcaact tattacagcc attggcggca cgcgttgcta
     1561 attcacgatg gtgattttat ttccacgcaa acggtttcgt cagcgcatca gattctttat
     1621 aatgacgccc gtttcccccc ccttgggtac accgaaagct tagaagacga gagacttatg
     1681 atggaaattc tgcgtggttc gcctgcattg tcggcattcc gaatcaacaa actgctggca
     1741 cgttttcagg ctgccaggct cccggttcac aatatttacg ccgagtatgt ccattttgct
     1801 gacctcaatg cgccgttaaa cgatgatgag cacgcacaac ttgaacgcct gctgaaatat
     1861 ggcccggcac tcgccagcca cgccccgcaa ggcaaactcc tgctggtgac cccgcgtcct
     1921 ggcaccatct ctccctggtc ttcgaaagcg accgatatcg cccataattg cggtctacaa
     1981 caggtaaacc gccttgagcg cggcgtggct tactatatag aagccggtac gctgaccaat
     2041 gaacaatggc agcaggtgac cgctgaactg cacgaccgca tgatggaaac ggtctttttt
     2101 gctttagatg atgcagagca actgtttgca caccatcaac cgactccggt caccagcgtt
     2161 gatttgctgg ggcagggccg tcaggcgcta atcgacgcta acctgcgtct tggcctggca
     2221 ctggcggaag atgaaattga ctatctgcag gatgctttca caaagcttgg tcgtaatccg
     2281 aacgacatcg aactgtatat gtttgcccag gcgaactccg aacactgccg ccacaaaatt
     2341 tttaacgccg actgggttat cgatggtgaa cagcagccga aatcgctatt taagatgatc
     2401 aaaaacacct tcgaaaccac gccggatcac gttctctctg cttataaaga taacgccgcc
     2461 gtaatggaag gttctgaagt gggccgctac tttgctgacc acgaaacggg ccgctacgat
     2521 ttccatcagg aaccggcgca tattctgatg aaagtcgaaa ctcacaacca cccgacggcg
     2581 attggcgcaa agccgaaagc tggtctggtt ggtttctccg tatccaacct gcgaattcct
     2641 ggcttcgaac agccgtggga agaagatttc ggtaaacctg agcgcattgt taccgcgctg
     2701 gacatcatga ccgaaggccc gctgggcggc gcggcgttta acaacgaatt tggtcgcccg
     2761 gcactgaacg gctacttccg tacttatgaa gaaaaagtga acagccacaa tggcgaagag
     2821 ctgcgcggtt atcacaaacc gatcatgctg gcgggtggga tcggccacat tcg
//
LOCUS       DAWDRC010000522         2862 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13300_length_2862_cov_1.857143, whole genome shotgun sequence.
ACCESSION   DAWDRC010000522 DAWDRC010000000
VERSION     DAWDRC010000522.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13300_length_2862_cov_1.857143"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1290..1299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2139..2148
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2564..2573
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          621 a          806 c          698 g          707 t
ORIGIN      
        1 atagccagtg cgagtattaa tgtgcctgac cgcgcatttt cgctactgct ttcactccca
       61 gcaccacgat gccgccgatg ataaatccaa gaatcagatt taaaacagtc ggtaatatca
      121 tcgccaccac tgcactttgc tgcccggcga tatgttcaat agcgtgatgc agcggcgcaa
      181 taccatgtac cacaatcccg ccgccgacga ggaacatcgc cagcgtgccg acaatcgata
      241 atgctttcat cagccagggc gcgataatca ataacccttt acctaatgcc tgcatcagcg
      301 cgctggattt ttccgccagc caatacccca ggtcatcaat cttaacgata acccctacca
      361 gaccgtagac gcccacggtc actaccagcg cgatgcctga aagcaccagc acctgattaa
      421 gcaacggcgc ctctgccaca atccccagcg tgatggcgac gatttccgca gacaaaataa
      481 aatcggtacg aatcgcccct ttaattttgt ccttttcaaa cttcagcgga tcctgcgccg
      541 ccagcttctc cagacgctgc tgactctgcg ccggatcttc tttatgttta cgcgcctcca
      601 gcatatgcag cactttctct accccttcaa agcagagaaa cgcgccacca atcatcaaca
      661 gtggcgtaat cgcccacggg ataaacgcac tgatgatcag cgccagcggc accagaatca
      721 ctttattgat cagcgatcct ttcgccacgc cccagaccac gggaagttcc cggttggccc
      781 gcacgcctga aacttgttgc gcattgagcg atagatcatc ccctaataca ccagcggttt
      841 tcttcgccgc cagtttgccc atcacggaga tatcgtccag cagtgtggcg atatcatcga
      901 gcaacgtcag taaactactc ccggccaaaa gcattccctc tcatttttat tgttgaaggg
      961 tgtaagtatg aagcaaaaac ggcagctctg atacttgctt cacaggtcaa catttattta
     1021 acaatcaagt gcaattatat ctctcgccag cagaatgatt ttcacgttta ctatgaacca
     1081 cctttttatt tccgccgtga gggattatgc gtttccgcca gttgttaccg ctttttggcg
     1141 cgctgtttgc gttgtatatc atttggggct caacctattt tgtcattcgg attggcgtgg
     1201 aatgctggcc tccgttaatg atggcgggcg ttcgattcct ggcagccggt attttattgc
     1261 tggcattttt gctactgcgc ggacacaaan nnnnnnnnnc cgctacgtcc gctgctcaat
     1321 gccgcgctga ttggcctgtt attgctggct gtcggtaatg gcatggtgac ggttgccgaa
     1381 catcaaaatg ttccttccgg catcgccgcc gtagtggttg caaccgtgcc cctctttacc
     1441 ctgtgcttca gccgcctgtt tggcattaaa acgcgcaaac tggaatgggt gggtatcgcc
     1501 attgggcttg ccggaatcat catgctcaat agcggtggaa atttaagcgg caatccgtgg
     1561 ggcgcgattc tgattttaat cggctcgatt agctgggcgt ttggctcagt ttatggctcg
     1621 cgcattacct tacctgtagg gatgatggcg ggtgcgattg agatgctggc ggctggcgtg
     1681 gtgttaatga tcgcatcgat gattgcgggt gaaaaactga cggcgctccc ttccctgtca
     1741 ggcttccttg cggtcggcta tctggcgctg tttggttcga ttatcgccat taacgcttat
     1801 atgtatttaa tccgtaatgt cagtccggct ctcgccacca gctacgccta cgttaacccg
     1861 gtggtcgcgg tcttgctggg tacgggactg ggtggcgaaa cgctgtcgaa aattgaatgg
     1921 ctggcgctcg gcgtaattgt tttcgcggtg gtactggtca cgttgggaaa atatctcttc
     1981 ccggccaaac ccgttgtcgc gccagttatt caggatgcat caagcgagta aatgaatccc
     2041 ctgcgtgtcg atctgcgcgc tggcaccttc gccgcagatc cactcttcca gacgctcggt
     2101 aagcgccgca tccgtaagct tctcgcgccc acgtaacgnn nnnnnnnnac tcccagacaa
     2161 tcagtacgcg ccagccgagt tcctgcaagc gactgatatc gcggcgatcg cgttcaacat
     2221 ttttacctat cttctccagc cagaactcgg ttcgagtcgc aggcacttta aacagatagc
     2281 agtgatgatg atgccagaag cagccgtggg taaatatcac gcagcgatat tcatcaacga
     2341 caaaatccgg gcgtccgggc agactggcgt cctgaacgcg aaacgcaagg ccctgcccgg
     2401 ttaacaaact ggcgaggcgc ttctcaatcg ccgtatcacg cgtggcaatc gcgcgcatat
     2461 ttttgctgcg ggtggcctta tcgtgaacgt cgaccatgtt gtgcctcttg ctgacgcaac
     2521 gccaccgcct gtttgatttt tggctcaagc agttttgcca ccgnnnnnnn nnngcaaaga
     2581 ccggcacgac caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc
     2641 ggaatttcgc ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta
     2701 accgacgtgg acgatgttgc tgattcagcg gatcgtcaaa gtctttctca cccgtggcca
     2761 tatcccagcc gcggtcgatt aaaatttccg cgccatcttt gtaataacga gcagacagcg
     2821 tacgggtgac gctttgcgga ttgttcggat aaaccattcc at
//
LOCUS       DAWDRC010000523         2861 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13311_length_2861_cov_1.975053, whole genome shotgun sequence.
ACCESSION   DAWDRC010000523 DAWDRC010000000
VERSION     DAWDRC010000523.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13311_length_2861_cov_1.975053"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          629 a          660 c          786 g          786 t
ORIGIN      
        1 ccaatatcga agcgaaatat catcttaatg atccgatcat gactcagtat ttcagctacc
       61 tgaaacaact ggcgcatggc gatttcggtc catcgtttaa atataaagat tattcggtca
      121 atgacctggt tgcatccagt tttcccgttt ctgccaaact gggagccgca gcattttttc
      181 ttgcggtaat actgggtgtt agtgctggcg ttattgccgc attaaagcaa aacaccaaat
      241 gggactatac cgtgatgggg ctggcaatga ccggggttgt tatccccagt tttgtggttg
      301 cgccattatt agtcatgata tttgcgatca ttttgcattg gctgccgggc ggtggctgga
      361 atggtggagc gcttaaattc atgatattgc caatggtggc gttatcactc gcctatatcg
      421 ccagtattgc gcgtattacc cgtggctcta tgattgaggt tttacactcc aactttattc
      481 gtactgcacg ggcgaaaggg ttacctatgc ggcggatcat tttacgccac gcattaaaac
      541 ctgctctgtt acccgtgctc tcctatatgg ggcccgcatt tgtcggcatt attaccggtt
      601 cgatggtcat cgaaaccatt tatggtttac cggggattgg gcaattgttc gttaatggcg
      661 cattgaaccg tgactattcc ttagtgttaa gtctgaccat tctggttggc gcgttaacca
      721 ttttgtttaa tgccattgtc gatgtgctat atgcggttat cgacccgaaa atccgttact
      781 gatactggag ctcgcgatga tgttaagtaa gaaaaacagc gagacgctgg aaaatttcag
      841 tgaaaagctg gaggtcgaag ggcgcagctt gtggcaggac gcacgtcgac gttttatgca
      901 taaccgtgcg gcggttgcca gtctggtagt gctggtgctg atcgcgttat ttgtaatcct
      961 agcaccgatg ctttcgcagt ttgcctatga cgatactgac tgggcgatga tgtccagcgc
     1021 cccggatatg gagtccggtc actactttgg tactgactca tccggtcgcg acctgcttgt
     1081 gcgcgttgcg attggcgggc gtatctcact catggtcggt gttgctgcgg cactggtggc
     1141 agtggtcgtg gggacacttt atggttcgct ttccggttat ctgggcggta aagtggattc
     1201 ggtaatgatg cgtctgctgg aaatcctcaa ctccttccca ttcatgttct tcgtcatttt
     1261 gctggtgacc tttttcggtc aaaacatcct gctgattttc gtggcaattg gcatggtttc
     1321 ctggctggat atggctcgta ttgtgcgtgg gcaaaccctg agtctgaagc gcaaagagtt
     1381 tattgaggcg gcacaagttg gcggtgtatc gacgccgggc attgttattc gccacattgt
     1441 gccgaacgta ctcggtgtgg tggtggtcta tgcgtcgctt ctggtgccca gcatgatcct
     1501 ctttgaatct ttccttagct tcctgggatt aggtacgcaa gagccgttaa gcagctgggg
     1561 tgcattgctg agtgatggcg cgaactcgat ggaagtctct ccatggttac tgttgttccc
     1621 agcgggattc ctcgtggtaa cgctgttttg tttcaacttt atcggcgatg gcttgcgtga
     1681 tgccctcgac ccgaaagatc gttaaggagt gcagccatga gcgtaattga aactgcaact
     1741 gtgccgctcg cacaacaaca ggctgacgca ctgctgaacg tgaaagattt gcgtgtcacc
     1801 tttagtaccc cggatggcga cgtcacggcg gtcaatgatt tgaatttttc gctacgtgcc
     1861 ggagaaacgc tgggcatcgt aggtgagtct ggttcgggca aatcgcaaac cgcatttgcg
     1921 ttgatgggcc tgttagctgc caacggacgt attggcggat cggcaacctt caatgggcgt
     1981 gaaatcctca atttgccaga gcgtgaactc aataaactgc gagctgaaca aatctcaatg
     2041 atttttcagg acccaatgac ttcgttgaat ccctatatgc gcgtcggtga gcagttgatg
     2101 gaagtgctga tgctgcataa gaacatgagc aaagctgagg cgtttgaaga atcggtgcgg
     2161 atgctcgatg cggtaaaaat gccggaagcg cgtaaacgca tgaaaatgta cccgcacgaa
     2221 ttttctggcg gcatgcgtca gcgagtcatg attgcgatgg cattgctatg tcgacctaag
     2281 ctgctgattg cggatgaacc cacgactgcg ctggacgtca ccgtacaggc gcagatcatg
     2341 acgctattga atgaactgaa gcgggaattt aataccgcca tcattatgat tacccacgat
     2401 cttggcgtgg tggcggggat ctgcgacaaa gtgctggtaa tgtacgccgg gcgcacgatg
     2461 gaatatggca acgcgcgcga tgtcttttat caacccgtac atccttattc tatcggtttg
     2521 ctcaacgcgg tgccgcgtct cgatgcggaa ggtgaaacaa tgttgactat tccaggtaat
     2581 ccgccaaacc tgctgcgatt accgaaaggt tgcccgttcc agccacgttg tccgcatgcg
     2641 atggacattt gtagtagcgc accgccgctg gaagagttta cgcctggccg tctgcgtgct
     2701 tgctttaaac cggtggagga actgttatga atgctgtaac cgaaggaaga aaagtcctcc
     2761 tcgaaatcgc cgatctgaaa gtgcactttg aaatcaaaga tggcaaacag tggttctggc
     2821 aaccgccgaa aacgctcaaa gccgtcgatg gtgtcactct t
//
LOCUS       DAWDRC010000524         2858 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13329_length_2858_cov_2.061720, whole genome shotgun sequence.
ACCESSION   DAWDRC010000524 DAWDRC010000000
VERSION     DAWDRC010000524.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13329_length_2858_cov_2.061720"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    456..465
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1186..1195
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1750..1759
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          648 a          729 c          733 g          718 t
ORIGIN      
        1 aattacatgt tttaagccac gcctggttta aggcattacc ggaagccgat gttgtgctcc
       61 agcgtccagt tccaaacgat atcgcctacc tccagtacac ctccggcagc acccgttttc
      121 cccgtggcgt cattatcacc catcacgaag tgatggctaa tctacgtgct ataagccacg
      181 atggcattaa attacgccct ggcgaccgct gcgtctcctg gctgcctttc taccatgata
      241 tgggactggt cggctttctc ctgacccccg tcgccacgca gctttcagta gattatttgc
      301 gcactcagga ttttgccatg cgtcctctgc aatggcttaa attgatcagt aaaaatcgtg
      361 gcaccgtttc cgttgcaccg ccgtttggct atgaattgtg ccagcgccgc gtgaatgaaa
      421 aagatctcgc tgaactggat ctttcctgct ggcgcnnnnn nnnnngctgg cgcgtcgctg
      481 gtattggtgc agaacccata tccgcagaac aactccatca attcgctgaa tgtttccgtc
      541 aggtcaactt tgacgataaa actttcatgc cgtgctacgg actggcagaa aatgcgctgg
      601 ctgtcagctt ctctgatgaa gcctgcgggg ttgtggttaa cgaagtggat cgcgacatcc
      661 tcgaatacca gggtaaagcc gtcgcgccgg gtgctgaaac acgcgccgta tcgactttcg
      721 tcaactgcgg taaagcgttg ccggaacatg gcattgaaat ccgcaatgaa gcagatatac
      781 ctgtcgcgga acgtgtggta ggccatattt gcatctccgg ccccagcctg atgagcgggt
      841 actttggcga ccaggtttcg caagacgaga ttgccgcgac gggctggtta gacaccggag
      901 accttggtta tctgctggac ggttatctgt atgtcaccgg acgcattaaa gatctgatta
      961 ttattcgtgg ccgtaatatc tggccgcagg atattgaata cattgcggaa caggagccgg
     1021 aaattcattc tggcgatgcg attgcttttg ttaccgccca ggaaaaaatc attttgcaga
     1081 tccagtgtcg gatcagcgac gaagaacgtc gcgggcagct tatccacgcg ctggcagctc
     1141 ggatccaaag cgaatttggc gttaccgcgg atatcgatct gttgcnnnnn nnnnncgccc
     1201 cacagtattc cccgaacatc ctccggcaag cctgcccgtg cggaagcgaa aaaacgttat
     1261 cagaaggctt atgctgccag tcttcatgtg caggaatccc tggcatgaat caaactgtcg
     1321 cggtgacggg cgctaccggg tttatcggta aatatattat tgataacctg ctcgcccgcg
     1381 gctttcacgt tcgcgcattg acgcgtactg cccgcgctca cgtcaatgat aatctcactt
     1441 gggtgcgcgg ttcgctggaa gatacacatt cgcttagcga actggttgcc ggagccagcg
     1501 cggtggtcca ttgcgccggg caagtgcgcg ggcacaaaga agagattttt acccgctgca
     1561 acgttgacgg cagcctgcgc ctgatgcaag cggcaaaaga gagcggcttt tgccaacgat
     1621 ttctgtttat ctcttcgctg gcggcgcgcc atcccgaact ctcctggtac gcaaattcca
     1681 aacacgtcgc cgaacaacgg ctgactgcaa tggctgacga aattacgttg ggcgtttttc
     1741 gcccgacagn nnnnnnnnnc cgtgtatggt cccggcgata aagagttaaa accgctgttt
     1801 gactggatgc tgcgcggtct gctgccacga cttggcacgc cagagacact gctctctttt
     1861 cttcacgtca ccgattttgc gcaagcagtg ggtcagtggt taagcgccga aactgtacag
     1921 acgcaaacct atgaattatg cgatggcgtc gccggaggct atgactggca acgtgtccaa
     1981 cagcttgttg ccgatgtccg ctgtggttcc gtgcggatgg tcggtattcc tctgccgcta
     2041 ctcacctgcc ttgcggatat cagtaccgcg ttgagccgtc tggcgggtaa agaaccgatg
     2101 ctgactcgtt cgaaaatccg tgaattaact catgccgact ggtcggcaag taataaccgt
     2161 atttctgaag atattaattg gtttcccggg attagcctgg aacacgcatt acgcaacggg
     2221 ctattttgat tgtcttgttt aaaggtgtgt taacgtttct atggtaaatc gtgaaatagt
     2281 aatggattac atcctttcct gtttacagga tttggtcgaa aacggtgtgg aaattaagcc
     2341 cgacagcgat ctcgtcaacg atcttggcct ggagtcgatc aaagtgatgg atctcttaat
     2401 gatgctggaa gacagattcg atatttccat tcctattaat attctgctgg atgtcaaaac
     2461 ccctgcgcaa ttaatggaaa ctctacttcc atggctggag aataaataat ggggctatac
     2521 gataaatatg cgcgccttgc tggcgagcgc ctgcaattta gtgacaacgg gttaacacca
     2581 tttggcacct gtattgatga agtatattcc gccaccgaag gccggatcgg caataaaaaa
     2641 gtgattctgg ccgggaccaa taattatctc ggtttaacct ttaatcatga tgctatccgc
     2701 gaaggtcagg cagcgttagc ggcccaaggc accggcacca ccggttctcg tatggcaaac
     2761 ggcagttatg cgccgcatct ggcgttagag aaagagattg ccgagttttt caatcgccct
     2821 accgctatcg ttttttcaac cggttatacc gctaattt
//
LOCUS       DAWDRC010000525         2854 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13346_length_2854_cov_2.570561, whole genome shotgun sequence.
ACCESSION   DAWDRC010000525 DAWDRC010000000
VERSION     DAWDRC010000525.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13346_length_2854_cov_2.570561"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          634 a          732 c          693 g          795 t
ORIGIN      
        1 ttcataaaat ctttagcaac atactggaaa agcgccgaca gttgcaaatg cgtttttacg
       61 cgatgcttcc tgctccacga cagggaggag aggcaaagac ggtgtcctgc aacccttccc
      121 actccttaat agtgtaagta tgcagagcca gcgcatgaac ggtagtagag agttcctccg
      181 ctaaagtact gtaaatcatt cgatgacgat tcagaaaacg ttcacccgta aaacgatcgc
      241 tgaccagcac aactttaaaa tggctttcag agccggctgg gacattgtga cgatagcttt
      301 catccactac ttcgaggaat acgggttgga acgccgccct taatttttct tctatccgct
      361 cacgtatcat catgaaatat ctcctccgac aacgctgaga tgtcatccat ccctttaaat
      421 actagccgct tttaccgttt ccattgcagc ttaacaacaa atatttaggt ttcgtcctgg
      481 tttttccgtc aagcgtatga agtttactga tttaaagatt gaaacacagg cctcgcatca
      541 gcgtttcttt taccaggggc gattatttcg tcaacatgat gaatttacat gccttaccca
      601 cttcccctcg ccgttggcaa tgttatgatg gcgggaattt ttaccaaaca cgcttaagag
      661 tctctgagag cccgaacatg ttcaaaaaaa tcctcttccc gttagttgct ctgtttatgc
      721 ttgcaggatg cgcaaaaccg ccaacaacta ttgaagtttc cccgacgatt acactgccac
      781 agcaggatcc aagcctgatg ggcgtcaccg taagcattaa tggtgccgat cagcgtaccg
      841 atcaggcgct ggcaaaagtc acccgcgata atcaaatcgt taccctgacc gcctcccgcg
      901 atctgcgttt cctgctgcaa gaagtgctgg aaaaacaaat gaccgcccgt ggatacatgg
      961 ttggtccgaa tggcccggtt aatctgcaaa tcatcgttag ccaactgtat gctgacgtgt
     1021 cccagggcaa cgtgcgctac aacatcgcga ccaaagcgga tatcgccatc atcgctaccg
     1081 cgcagaatgg caacaaaatg accaaaaact atcgtgccag ctacaacgtt gaaggtgcgt
     1141 tccaggcctc caacaaaaat atcgccgatg cggttaacag tgtgctgacc gataccatcg
     1201 ctgatatgtc tcaggacacc agcatccacg aattcatcaa gcagaacgcg cgttaatttc
     1261 tgccctctgg cccggtgcaa gccgggcctg tagacgccca tgtccagtca atatttacgt
     1321 atttttcaac agccgcgttc agccatattg ctgatcctgg gttttgcttc cgggctaccg
     1381 ctcgccctga cgtccggcac cttacaggcc tggatgacgg tcgagaatat cgatctcaaa
     1441 accattggtt tcttctctct ggtaggccag gcttacgttt ttaaattcct ctggtcacca
     1501 ctgatggacc gctacacgcc cccatttttc gggcggcggc gcggttggct gctcgccacg
     1561 caaattctgt tattagtccc cattgcggcg atattgtctt cgatgcgtgg aaaaccgatg
     1621 tgctcccggc agaagaacgt ggtgcgggcg cggcaatcag cgtgctgggt taccgtttag
     1681 ggatgctggt ttccggcggc ttagccctgt ggctggcaga taaatggctg ggctggcagg
     1741 gcatgtactg gttgatggcg gcgctgttga tcccctgtat tatcgcgacg ttgcttgcac
     1801 cagaacccac cgacaccatt cctgtgccca aaacgctgga acaagcggtt gttgcacctc
     1861 tgcgagattt ctttggtcgc aataatgcct ggcttatttt gcttcttatc gtgctgtata
     1921 agctgggcga cgcattcgct atgagcctga caaccacgtt tttgattcgc ggcgtcgggt
     1981 ttgatgcggg tgaagtaggc gtggttaaca aaacgcttgg cttactggcg accattgttg
     2041 gcgcattgta cggtgggatt ttgatgcagc gcctgtcgct gttccgtgca ctgctgattt
     2101 tcggcatttt acaaggtgca tctaacgccg gttactggct gctgtcgatt actgataagc
     2161 atctctacag catgggtgca gccgtctttt tcgaaaacct ctgtggcggg atgggtacat
     2221 cagcctttgt cgcgctgtta atgacgctgt gtaataagtc attttccgct actcaatttg
     2281 ccctgctctc agcgctttct gctgtagggc gagtttatgt cggccccgtg gcgggttggt
     2341 ttgttgaagc acatggctgg tcgacattct atctattctc cgtcgccgct gccgtaccag
     2401 ggcttatttt gctgctggtt tgccgccaga cgcttgaata tacacgagta aatggcaact
     2461 ttatctcccg taccgaatat cctgcaggtt atgcctttgc catgtggaca ctggcggcgg
     2521 gtatcagcct gttggccgtg tggttactgc tgttgacaat ggacgcgctg gatttgacgc
     2581 acttctcttt cctgcctgcg ctgctggaag tcggggtttt agtcgccctt tctggcgttg
     2641 tgcttggtgg tttgctggat tatctggcgc tacgaaaaac acatctgacg taatctgtaa
     2701 atattattta gctgtagtaa tcactcgccg taattattac ggctaaataa acatcagcat
     2761 cgcttattaa tatttgttct tttgtgcggc ttagcgtttg gaaattattg acgcaattta
     2821 tgaattctat ttttcttaca cgattcagct aatg
//
LOCUS       DAWDRC010000526         2851 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13365_length_2851_cov_2.027897, whole genome shotgun sequence.
ACCESSION   DAWDRC010000526 DAWDRC010000000
VERSION     DAWDRC010000526.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13365_length_2851_cov_2.027897"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1253..1262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          771 a          594 c          788 g          688 t
ORIGIN      
        1 tcaggggcag aatttacaaa agaaaaatac cctatatcgc ataatgcgat taaatacgcc
       61 atcttataga aaatgatggt tagatgaata tgcggattat ctctctacgc aaggagtatt
      121 tatctttact cccgtcaatg attgcatctc ttttctctgc taacggtgcc gcagcggtca
      181 ttgattcatg ccagggatat gatatcaaag cgaactgtca ggccagcagg caaagccttt
      241 caggcaatat gcaggactgg agcatcgcgg atgggcaatg gttgattttt tcgggtatgg
      301 ccaataatgc cagcggtggc gcagtatttt tgcagcacag tgctgaattt acgatatcac
      361 cacaaaatga aacagggatg accctgtttg ctgataactc ggttagtggc gaatataata
      421 atggtggggc tatctttgct aaagaaaact caacgataaa tcttgcgaat gttattttcg
      481 acagtaatgt tgcaggaggc tatggggggg caatttattc tgccggtacc aatgataccg
      541 gtgccgccga tttacgtgtc actaacgccg tatttcacaa taacatcgct aatgacggca
      601 aaggcggtgc aatttatacc atcaataatg acgtctattt aagtgatgat gcttttaata
      661 ataaccaggc atatacatca acaagttata gtgatgggga tggcggcgct attgatgtta
      721 cggataatag tacggataac acacatcttt caggaaaaac gataattaat aatacctcct
      781 ttaccaataa ctatgcagaa ggttatggcg gagcaattta taccagcagc accacatccc
      841 cgtatcttat tgatatttct gttgatgata attatgacca aaacaatggt gtgatgattg
      901 atgaaaataa cagcgcctcg ggatatgacc attcggcgac ggcagcggcg gggggcttta
      961 tgtatatcgg tcatagtgtg gctgaattta acattgccgc cgataaaacg ctggtaatag
     1021 gcaacaccag caacgatggt gcgattgatt ctcttgccgg aacgggagtc atcgtgaaag
     1081 agggagcggg agagttagtc ctcaatgccg acaataatgc gtttaccggc gagatgagta
     1141 tccagaatgg cgaggtgacg ctggggcgca gcgatgagtt aatgaatgtc ggcgacacgc
     1201 actgtcagag cgatccgcag gattgctttg gtctgatggt cggcagcact gtnnnnnnnn
     1261 nntgagtatc aggcagaact gaatgttggc aatacacaac aaacgtttgt gcactcatta
     1321 accggttttg ctaatggcat tttgaatatc gacgcaggcg gcaatgttac agtaaaccag
     1381 ggcggttttt ccggctcgat tcagggtgaa ggacagttga cggtagcgca agatggcagc
     1441 tatctcctga caggggcgca gtcgatggca ttaaccggcg atattgtggt ggaagataac
     1501 gccgtattgt cgctggcggg gaatcaggcc gatttacggg caatgcagag cgatccgcaa
     1561 tcgatcgtgc taaatggcgg agtattggat ctctctgatt tcaccacctg ggatggcgat
     1621 agctcataca atgacggtct gcaaatcagc ggcagcgggg ggacagtgat tggcagtaat
     1681 gatgtcgttg atatcagcag tggcgatgat ttacatattg gcggcagtga tgccagccag
     1741 aatggcgttt atgtcgtcat taatgcgggg gatcagcgcg taacgctggc gaataacaac
     1801 ggttatctcg gtaatacgca aatcgcctcc ggtacgcttg aagtcagcga taattcgcaa
     1861 ttgggggaca caagttacaa ccgttcagtg atcttcacag atccccaaca acacagtgaa
     1921 atggatgtga cgactgatgt tgacactcgc tcggctacaa caggtcaggg caggaatatt
     1981 gaaatgcgcg ctgatggcga aatacacgtt gaggatggcg tagatacgca atggggcggg
     2041 ttgatggcgg atagtaccgg gcaacaactg gatagcgtaa gcacgttgac taaaagtggc
     2101 ggcggtacgc tggagttgac cgccagcggc actgcgacgt ctgcggtacg tgtggaggac
     2161 ggaacgctaa aaggtgaagc ggagaatatt attccttatg tttcttcact gtgggtgggg
     2221 gaagacggtg tttttgaaac cgggcaaaat caggatattc gctcaatcga tgccacctct
     2281 ggcggcgata tcgacataac cgatggtact gtgttgcgat tgacgcagca gaatacgaac
     2341 caggcgctgg atgcctcgct gtttagcggc gacggtacgc tggtgaatgc caccgatggc
     2401 gtgacgctgg caggtgaact taataccaac cttgaaactg acagcctgac ttatctttcc
     2461 gacgtgacgg ttaatggcaa tctgaccaat acgtccggtg ctgtcagtct gcaaaatggc
     2521 gtcgctggcg acacgctgac ggtaaatggt gattataccg gcggcggtac gctactgctc
     2581 gacagcgaat taaacggcga tgactcggca agtgaccaac tggtattgaa cggcaatacc
     2641 tcgggcaaca cggccgtggt aattaacccc attacgggta ttggtgagcc gacatctaca
     2701 ggtattaaag tagttgattt cgcagctgat cccgcgcagt ttcaaaacaa tgcgcagttc
     2761 agtctggcag gaagcggcta cgtcaatatg ggggcgtatg actacacgct ggtggaagat
     2821 aacaacgact ggtatctgcg atcacaagaa g
//
LOCUS       DAWDRC010000527         2847 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13385_length_2847_cov_2.359241, whole genome shotgun sequence.
ACCESSION   DAWDRC010000527 DAWDRC010000000
VERSION     DAWDRC010000527.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13385_length_2847_cov_2.359241"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1897..1906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          652 a          734 c          722 g          729 t
ORIGIN      
        1 gccgatagtt gagcggctgg cacgtttgca ggagccagac ctcccggaaa aggccgtgcg
       61 atatacccgc cgcgtaacgc aggtctggtg cctgtttttt attttcaatg gcagtattgc
      121 gttatttacc accttgtatg gcaacatgcc cttgtggaca gcctggaacg gtatcattgc
      181 ttaccttctg ataggtttcc tgactgcagg ggaatggctg atacgtcgta agatgatcaa
      241 acggaaaaca ccatgaatca gactctctca ctttcacagt ggttgacagc ctctcgccct
      301 gtaacaactc ctgttgcctg gcttggggaa tatacctgga cactggggca tctgcggcat
      361 gatgtagcgc ttttgatcga ccatttacgc gaccagccag gcaatcgctg ggcactttgt
      421 tttgaaaaca gctatctttt tattgtagcg ttgttggcca cgctacactc aggtaaaatc
      481 ccggtaatcc cgggccataa tcgcgccgct ttgttaaatg tacaacgctc cttgtttgat
      541 tgcgtcctga gtgataaaag gctgggctgg aacggtccac agtttgttgt aagcacatct
      601 caccagatga cgaccagcac gatagccttt gatgatattg cttctgacgc gtttattgaa
      661 cttttcacat cgggttcaac cggacaacca aaacaaattg caaaacctct tatcagcctt
      721 gatcaagaag caaacatgct ggcaactcac tttgcagacc gactactggg ctgtcgtttt
      781 gtagcatccg tgatgcctca gcacctgtat gggctgactt tccgtatttt tttacccatg
      841 gcacttggcc tgccattgca tgccgcaatg ctctggtatt ctgagcaact tgccgcatta
      901 agccatacct accgctatgc ctttgtcagc agcccggcgt tccttaagcg actcgacctt
      961 catctgacgc ctcccccagt ggaaatgatt ctctctgccg gcggtatgct accatggcag
     1021 gatgtagaga aaacttccac ctggctgaat atatggcccg atgaaatcta tggtagtacc
     1081 gaaacaggta ttctcgcatg gcgttaccgt caacaggata atatcccctg gtttactttt
     1141 tctgatgttc atctttcaca ggaaagtgag ggggttcgtg tcttttcgcc actaatccct
     1201 gctgaaggat tggtgcttga cgatatgttg caattcaatg aaaacggtca gttccacctg
     1261 atcggacgac gtggtcgagt ggtaaaaata gaggagaagc ggatatcact ggttgaaatc
     1321 gaacaacgac ttctggcact tgacggtatt atggatgtcg ctgcaatacc attaatacgc
     1381 aatggtcgcc aggctatcgg tgtcgtggtt gtaccaaata aagaggcccg tcaaatatgg
     1441 caatgctccg gagggaaaac tctggagttg tcctggcgca aagcactatt accatggctt
     1501 gaacctgttg cagtacctcg ttactggcgc attattgatg aaataccagt aaacaatatg
     1561 aataagcgtg tctatgcgca attacaggag ttatttcatg atacgccatg aaattgagcg
     1621 ccatctggtt caaccgcagg agatggaaat cgttctatac cttgacccga tgctctactg
     1681 gtttaatggt cattttgccg tacagcctct gttaccaggc gtggcccagt tggactgggt
     1741 aatgcattat gctaccacgc tgcttgctcc gggctggcgt tttcgtagta tccagaatgt
     1801 taaatttctg gcgccactta tacctgagac gactgttacc ctgcaattaa catggcagga
     1861 aacaagtcag gtactcactt tctgctatca gcgtcannnn nnnnnnccat gaaataaact
     1921 ggagagaaaa aaggtaggta accctctttc tcttatcggc tgcacgcggg tgacgcccgc
     1981 cacaccgcca gtagcgggaa gatccgccta tgtcggtaaa cttttctccc tgcgtgttga
     2041 tcccctgcta caaccacggc gcgatgatgc cgggcgtgct ggcgcgtctt aagccattta
     2101 atctgccctg tattgtggtg gatgacggca gcgatgccgc cacgcaacag caactggata
     2161 atctggttag cgaacagcct ggcgtgacct taattcgcct ggcagaaaac gcaggtaaag
     2221 gcgcagcggt gatgcgtggc ttgcaggcag cggcagacgc agggttcagc catgcggtgc
     2281 aagtggatgc tgacggtcaa catgcgattg aagatatccc caaactgctg gcactcgctg
     2341 aacaacaccc tgcggcgctg atctccggtc agccaattta cgatgactcc attccccgct
     2401 cacggctgta cgggcgctgg atcacccacg tctgggtatg gatcgaaacg ctctccctgc
     2461 aactgaaaga cagcatgtgc ggttttcgcg tttatccggt tgcgccgacg ctgcaactgg
     2521 caaaacgcgc caccatcggc aagcggatgg atttcgacac cgaagtgatg gtgcgcctct
     2581 actggcaggg aaataccagc tatttcgtgc cgacccgcgt cacctatccg ctggacgggc
     2641 tttcgcattt tgatgccctg aaagataacg tccgcatctc gctcatgcac acgcgtctgt
     2701 ttttcggcat gttgccgcgt attccttcac tgctgatgtg ccactcttcc agccactggg
     2761 cgcggcagag tgaagttaaa gggttatggg gaatgcgcct gatgctgctg gtctggcgtc
     2821 tgctgggaag aacggcgttt accgcgc
//
LOCUS       DAWDRC010000528         2846 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13393_length_2846_cov_2.852024, whole genome shotgun sequence.
ACCESSION   DAWDRC010000528 DAWDRC010000000
VERSION     DAWDRC010000528.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13393_length_2846_cov_2.852024"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          658 a          745 c          778 g          665 t
ORIGIN      
        1 atggggatgg cgattgcgcc gtttgacaag ccgcatccgg taatgcggat tcagccgatt
       61 gccattagtg cctctggtgc tttgccagac acattaagta gcctgcctgc gttaccttcg
      121 ctggaggggc tgacggtacg caagctgcaa ctttctatgg acccaatgct cgatatgatg
      181 gggatgcaga tgctgatgga gaaatatggc gatcaggcga tggccgggat ggatcacagc
      241 cagatgatgg gccatatggg gcacggcaat atgaatcata tgaaccacag cgggaagttt
      301 gatttccacc atgccaacaa aatcaacggt caggcgtttg atatgaataa gccgatgttt
      361 gcggcggcga aagggcagta cgaacgttgg gttatctctg gcgtgggcga catgatgctg
      421 catccgttcc atatccacgg tacgcagttc cgtatcttgt cagaaaatgg caaaccgcca
      481 gcgacgcatc gcgcaggctg gaaagatacc gttaaggtag aaggtaatgt cagcgaagtg
      541 ctggtgaagt ttaatcacga tgcaccgaaa gaacatgctt atatggcgca ctgccatctg
      601 ctggagcatg aagatacggg gatgatgtta gggtttacgg tataaaaaga ccgtattcgg
      661 aaatatgccc ggatctctcc gggcatgaaa ctgatgacaa acgcaaaact gcctgatgcg
      721 ctacgcttat caggcctacg cgcaatgtat tgaatttgca aatgttttgt agttttgtag
      781 gccggataag gcgttcacgc cgcatccggc atgaacaaat cgcacgttgt cagcaatctg
      841 agcaagcctt acttcacatc atccggcagc gcataagcca caatatagtc gcccatcttc
      901 gtaccaaatg aaccgtgacc gcctgcggag atcaccacat actgcttgcc attcacttca
      961 taggtcattg gcgtagcctg accacccgct ggtaaacgac cctgccacag tttttcaccg
     1021 ttgctcatgt tgtaagcgcg caggtagtta tctgccgtag cggcgataaa cagcacgtta
     1081 cccgccgtgg agattggccc gccaagcatc ggcataccca tattgaacgg caccggtaca
     1141 ggcatcggga atggcatact gtcctgcggc gtaccaatac gtttcttcca caccacttca
     1201 ttggttttca gatccaatgc tgagatatag ccccatgctg gctgtttaca tggcagacca
     1261 aacggtgaga ggaacgggtt gagcgtgacg ccatacggta caccgtactg cggctggatg
     1321 ccggattccg tacccgtgcc tttggcatct ttcggctgtt ccatcgggtt acccggaccg
     1381 cgtgggatca gtttcgaaac aaacggcagt gccattgggt tggcaatcgc cacttcacga
     1441 ttcggatcaa cggaaatccc gccccattcg aacatcccca gattacccgg gaagaccagt
     1501 gtaccctgtt cagatggcgg cgtaaaaatg ccttcatagc gcatctggtg gaacatcacg
     1561 cggcacacca actggtcaaa catggtggca ccccacatat ccgcaccgct caaatctttc
     1621 gtcggacgga agctcagttc cgagaagggt tgagtagggg tcacgtaatc gcctttggca
     1681 gcaccttggg gaaccggttt ttccggggcc ggaaccacca gttcaccatt acgacgatcg
     1741 agcacaaaaa tgttgccggt tttcgccgga gcgtaaataa ccggcacttt ctgaccatta
     1801 acggtgatgt ccgccagcgt cggctgtgcc ggaagatcca tatcccacag gtcgtggtga
     1861 acggtctgat agctccacgc cagtttcccg gtagtggcat tcagcgccag aatcgagctg
     1921 gcataacgtt cctgttccgg tgtgcggtta ccaccccaga tatctggcgt ggtgacgccc
     1981 atcggcagat agaccagatc cagcttcgcg tcataggccg ctggtgccca cgagtttggt
     2041 gagttaaagg taaaggtgtg ttcgtcagac gggatcgcat tcggatcttt cgcgcccgga
     2101 tcaaaagccc acagcagctc cccggtgttg acatcaaaac cacggatcac gccagacgtt
     2161 tcgcgggttg agaagttgtc ggtgactgaa ccggccatca cgatggtttt atcggtgata
     2221 atcggcggcg aagtcggttc atacagaccc ggtttggtgt ccggcatatt gctttgcaga
     2281 ttgagcacgc ctttattggc gaaggtttcg cacagcttgc cgttttcagc gttaattgca
     2341 atcagacgac catcattgac cggaagaatg atacgacgcg ggcaatccgc catcacttcc
     2401 ggcgaagcag tttctgcttt ggcttcatga taagagacgc cacggcaggt tacatgctgg
     2461 aaggactcgt tggttttcag ctcaggatcg taatgccatt tctctttacc gctggcggca
     2521 tccagcgcaa acagacgttg atgcgcggta cacaggtaaa gggtgtcgcc cactttaatt
     2581 ggcgtcactt cattggtgat ttcgcccgga tcgttcggct gcttcacatc gccagtacgg
     2641 aacacccagg cttctttcag cttgtggacg ttatcagcgt taatttgctt cagtggtgaa
     2701 aagcgttgac cttcctgatt gcgaccatag gcaggccagt cctgatcggc aacgggggag
     2761 atggcttctg ctggcgtggt atcggcgctt aaggtgccgc tgatttcctg cggatcgtta
     2821 aatccggccc aggtcaggat accacc
//
LOCUS       DAWDRC010000529         2841 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13419_length_2841_cov_2.426059, whole genome shotgun sequence.
ACCESSION   DAWDRC010000529 DAWDRC010000000
VERSION     DAWDRC010000529.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13419_length_2841_cov_2.426059"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1301..1310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2322..2331
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          777 a          532 c          679 g          833 t
ORIGIN      
        1 tttattatcc tggtggtaat tttttgaacg catcagataa acttttttat ctgccgtttg
       61 ctttcattat tatcgtattg ctggtggttt atttaatgac cactcgagtg ttccgtcggc
      121 aattttctga aatgacagat ttggtcaata cgctggcgtt tttgcccgac tctacggatc
      181 aaatcgaggc tctgaagatt cgtgaaggcg atgcgaaaga gattatcagc atcaaaaatt
      241 cgatcgcgga aatgaaagat gctgaaattg aacgggcaaa taaattgctc tcgctgatct
      301 cttacgatca ggaaagcggt tttattaaaa atatggcgat tattgagtcc aacaataatc
      361 agtatctggc tgtggggatc atcaaactgt gtggtctgga agctgtggaa gcggtgtttg
      421 gtgttgatga acgcaataaa atcgtcagga aattgtgtca gcgaattgcc gagaaatatg
      481 cgcaatgctg cgatatcgtg acatttaatg ccgatctcta tttactcctg tgccgggaaa
      541 atgtacagac gtttacccgt aagatagcga cggtaaacga ttttgacagc agttttggct
      601 accgcaatct gcgcatccat aagtctgcta tttgtgaacc tttgcagggg gaaaacgcct
      661 ggagttacgc agaaaagctg aaactggcga tttccagtat ccgcaaccat atgttctcag
      721 agtttatttt ctgtgatgat gcgaaactca acgaaataga agagaatatc tggattgcgc
      781 gtaatattcg ccatgcaatg gaaattggcg aactattcct ggtctatcaa ccgattgttg
      841 atattaacac ccgcaccatt ttgggcgcgg aggcgttgtg ccgttgggtg tctgcggagc
      901 gggggattat ttcaccgctg aagttcatta ccattgctga agatatcggg tttatcaatg
      961 agctgggcta tcaaattatt aaaaccgcga tgggtgaatt cagacatttt agtcagcgtg
     1021 cgtcgctgaa ggatgatttc ttactgcata ttaatgtttc gccctggcag ttaaacgaac
     1081 cacactttca tgagcgtttt actaccatca tgaaagaaaa tggcctgaag gcgaacagcc
     1141 tttgtgttga gatcaccgaa accgtgatcg agcgaattaa tgaacatttc tatctcaata
     1201 ttgaacaact gcgtaaacaa ggggtacggg tatcgattga tgactttggc accggtttgt
     1261 caaacctgaa acgtttttat gaaattaatc cagacagtat nnnnnnnnnn agacagtata
     1321 aaggtggatt cgcaatttac cggcgatatt ttcggtactg caggaaaaat tgtgcgcatt
     1381 attttcgatc tggcacgcta taaccggatc ccggtgattg cggaaggcgt agagagcgaa
     1441 gacgttgcgc gtgaattaat caaattagga tgtgttcagg ctcaggggta tctgtaccag
     1501 aaacccatgc cattctccgc ctgggataaa agtggaaaat tagtaaaaga gtagtttacg
     1561 tatgtccaga atcaataaat tcgtacttac agtcagtctg ctgattttta tcatgatttc
     1621 agcagttgcc tgcgggatct acactcaaat ggtaaaggaa cgggtgtatg gcctgaaaca
     1681 gtccgttatt gatactgctt ttgcggtggc aaatattgct gaatatcggc gtagtgtggc
     1741 aattgatctc atcaacacgc taaatcctac ggaggaacag ctgttggttg gcttacgcac
     1801 agcttacgcc gactcggttt caccctctta tttgtacgat gtcggtcctt atctgatttc
     1861 cagtgacgaa tgtattcagg taaaggagtt cgagaaaaat tattgtgcag atattatgca
     1921 ggttgttaag tatcgacatg tcaaaaatac agggtttatc tcttttgatg gtaaaacctt
     1981 cgtctattac ctctatccgg taactcacaa tcgtagtctg atatttttgc ttggtctgga
     2041 acgtttttct ttactgtcaa aatcgctggc gatggatagc gagaacctga tgttctccct
     2101 gttcaagaac ggtaaatcgg tgaccggtga tgaatataac gctaaaaacg ccatcttcac
     2161 cgtttcggaa gcaatggagc acttctccta tttgccgacc gggttgtacg tatttgctta
     2221 taaaaaagac gtttattttc aggtatgtac attgattatt ttctttgccg cattggtggc
     2281 agtgatatcg ggtgccagtt gcctctatct ggtacgcaga gnnnnnnnnn naagaagcca
     2341 ttattaataa ccattttgaa cgcgtactgg atggcgggct tttcttttcg gctgccgatg
     2401 tcaaaaaact ctacagtatg tataactcgg cattcctgga cgatctgacc aaagcgatgg
     2461 gcagaaaatc ttttgacgaa gatttaaaag cgctgccgga aaaaggcggt tatttgtgcc
     2521 tgtttgacgt cgataaattc aaaaacatta acgacacctt cggtcatttg ctgggcgatg
     2581 aagtgttgat gaaagtggtg aaaatcctta aatcacagat cccggtagat aaaggtaaag
     2641 tttactgctt cggaggtgac gaatttgcgg tgatttatac ggggggaacg ctggaagagt
     2701 tgctatcgat tctaaaagaa atcgttcatt tccaggtggg aagcattaat ttaagtacca
     2761 gtatcggtgt agcgcattca aatgaatgtc ctaccgtcga acgcttgaaa atgctggcgg
     2821 atgagcggct gtataagagt a
//
LOCUS       DAWDRC010000530         2839 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13432_length_2839_cov_2.106681, whole genome shotgun sequence.
ACCESSION   DAWDRC010000530 DAWDRC010000000
VERSION     DAWDRC010000530.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13432_length_2839_cov_2.106681"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    373..382
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          687 a          705 c          693 g          744 t
ORIGIN      
        1 tcgtacagcc taacttctgg tggctgttgg caattaccgt cttgtttggc tggatgagtc
       61 tggtcggtgt ggtgcgggcg gagtttttac gtacccgtaa tttcgactac atccgcgcgg
      121 cgcaggcgct tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg caaaatgcca
      181 tggtcgctac cctcactttt ttaccgttta ttttatgtag ttcgataacc accctgacct
      241 cactcgattt cctcggcttc ggtctgccgc tcagttcacc gtcactcggt gaactgctgt
      301 tacaagggaa aaataacctt caggccccgt ggcttgggat caccgccttc ttatcggtgg
      361 cgatattgtt gtnnnnnnnn nnccgtccgc gacgcatttg atcctaataa ggcggtgtag
      421 catgacgcaa actctgttag cgattgaaaa tttgtcggtg ggttttcgcc atcagcaaac
      481 cgtacgtaca gtagtcaatg atgtttcact tcagattgag gctggcgaaa cgctggcgct
      541 ggtaggtgag tctggttcag gtaagagcgt taccgcgttg tcaattttac gcctgctccc
      601 ttgcccgccg gttgaatatc tctccggcga tattcgtttt catggcgaat cgctgcttca
      661 tgccagcgat caaacgttgc gcggtgtacg cggtaataag atcgccatga tttttcagga
      721 gccgatggtg tcgttaaatc cattacatac cctggaaaaa cagctttatg aagtactttc
      781 actccaccgc gggatgcgtc gggaagcggc tcgtggcgaa attcttaact gccttgatcg
      841 cgtcggtatc cgccaggcgg caaaacggct gacagattat ccgcatcagc tctccggcgg
      901 cgaacgccag cgggtgatga ttgcgatggc gctgttaacg cgaccggaat tattaattgc
      961 cgatgaacca accaccgcgc tggacgtctc tgtccaggcg cagattttac agctgttgcg
     1021 cgaactgcaa ggcgagctga atatgggtat gctgtttatt actcataacc tcagcattgt
     1081 tagaaaactg gcccaccgcg tggcggtaat gcaaaacggt cgctgtgtcg agaaaaataa
     1141 cgccgctacg ctgtttgcct cacccactca tccttacaca caaaagctac tcaacagtga
     1201 accatctggc gacccggtgc cattgccaga acctgcctct acgttgctag atgttgaaca
     1261 acttcaagtt gccttcccca ttcgtaaagg gatcttgaag cgcattgtgg atcataatgt
     1321 ggtggtgaaa aacatcagtt ttacgctacg ggcgggtgaa acactgggtt tagtgggcga
     1381 gtccggttcc gggaaaagca cgacgggact ggcgcttctg cgactgattg actctcaggg
     1441 cagcatcgtc tttgacggtc agccactgca aaatttaaat cgccgccagc tgttacctat
     1501 tcgtcatcgc attcaggtgg tatttcagga tccaaactcc tcactcaacc cacgactcaa
     1561 cgttttgcaa attattgagg aaggcttacg ggttcaccag ccgacgcttt ctgccgcaca
     1621 acgcgaacaa caagtgatag ccgtgatgca tgaagtggga ttagatcctg aaacacgcca
     1681 ccgttatccg gcggagttct ctggtggtca gcggcaacgt attgcgattg ccagggcgtt
     1741 aattcttaag ccctcgctga tcattcttga tgaaccaaca tcatcactcg acaaaacggt
     1801 gcaggcgcaa atattgacgc tattgaaatc attgcaacaa aagcatcaac tggcctattt
     1861 gtttatcagc cacgatttgc acgttgtccg cgcgttatgt catcaggtta tcgtactgcg
     1921 acaaggggaa gtagtggaac aaggaccgtg cgcgcgtgtg tttgccgcac cgcagcagga
     1981 gtatacgcgt cagctactgg cgttgagctg acgcttaaaa aggattgtag tttgaaaagg
     2041 gttcggcaat tgccacacca aaatttttca atcagcatgt tgcagcaaat tcgtcctggc
     2101 gatttacaaa caggcacggc tcaccttcac actccagcac cgaacatgga acttcgatgt
     2161 cgcttaacgc ctggcttagt ttgtacatca ccgaccatgc attatcacca tccgggctaa
     2221 gcaatttaag cgccaccagg ctgttgtcac cgcacggtcc attttgcgga atcggttcaa
     2281 ccttcgaacc tgcgaaacca gcagaccagc gatagttctg cggcagtcga tggacgattg
     2341 agagttgtaa tgaagtcact tattttcccc ggaagtacat tttacttcac aatttgttta
     2401 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
     2461 cgcctgcgtg gcctctatgg ctctgattta aataatatct gcgggcgtgg tcacgtttgc
     2521 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
     2581 tttttaaata aattgatttt acataaaata aacatatatc gggggaatga tagatttgtg
     2641 gttgacatgc atcaacaaag gaagcctttt agcttcctcg ttgtgcaata gatcaccgtt
     2701 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
     2761 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagtaatgcg ccaacaattg
     2821 cccctatgcc gaaacggaa
//
LOCUS       DAWDRC010000531         2824 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13509_length_2824_cov_2.397616, whole genome shotgun sequence.
ACCESSION   DAWDRC010000531 DAWDRC010000000
VERSION     DAWDRC010000531.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13509_length_2824_cov_2.397616"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          577 a          789 c          834 g          624 t
ORIGIN      
        1 gatatcgtgg tgcggcggcg gggagatcag cgtcactccg ggcaccgaat agcgcagttt
       61 ggcgatgtaa ggagtgactt tgtcgcccgg caactgaccg ccttcgcccg gcttcgcgcc
      121 ctgggcgact ttaatctgaa tcacgtcagc attgaccagg tacgccggag taacgccgaa
      181 gcgaccggac gccacctgct tgatgcgcga cactttgtta gtgccatagc gcgccgggtc
      241 ttcaccgcct tcgccggagt ttgagttacc gccgatgctg ttcatcgctt ccgccagcgc
      301 ctcgtgggct tccgggctta acgcgccgat agacatcgcg gcggtatcga agcgtttaaa
      361 cagttcgctt gccggttcaa catcagcaat gttgaccgcg ttttcacccg gcgtaattgc
      421 cagcagatcg cgtagcgtgg ttgccggacg ctcattaacc agcttcgcgt attcctgata
      481 gtcgctgtac tcgccgcttt gtaccgcctg ttgcagtgtg cgcacaacgt ccgggttgta
      541 ggcgtggtat tcgccaccgt gcacgtattt cagcaagccg ccctggctga tgggcttacg
      601 cgccagccag gcacgtttcg acagattcag cagatcctgc tggaagtctt caaagctcgc
      661 cccgccaatg cggctaaccg cgccctggaa gcacaggcct acgacatcat cgtgcagacc
      721 gaccgcttca aacagtttcg agcagcggta agaggcgatg gtggagatgc ccattttgga
      781 catgattttg tacaagcctt tgttgatgcc gttacggtag ttgagcatca cggtacgata
      841 atctttggca atcgcgtggg tgtctaccag gcggcccagc gtttcatagg caaggtacgg
      901 ataaatagcc gtcgcaccga agcccagcaa cacggcgaag tggtgcggat cacgggcgct
      961 ggcggtttct acgatgatgt tggcatcgca acgcaggctt tgctcgacaa gacgggtctg
     1021 gatcgcgcca accgccatcg gagccggaac cggcaggcga tctttagcga tattccggtc
     1081 ggagagcacc agcagcacgg tgccgctacg taccattttt tcggctttgt cacacagttc
     1141 tttcaccgtc gcttcgagcg tggttttagt gacgtcaaag gtgatatcca gcgtatctgc
     1201 gcggtagtga tcctctttca tcgtcgtgag ctgtttgaaa tcggagtaga gcagaatcgg
     1261 cgatttaaag cttaaacggt gcgcctgtcc ctctgcttcg cagaagacgt tcatttcgcg
     1321 gccaatgctg gtggcgagcg acataacatg cgcttcgcgc agcgggtcga ttggcgggtt
     1381 ggttacctga gcaaactgct ggcggaagta gtcgtaaata atgcgcggct ggctggagag
     1441 cacggcgaat ggggtgtcgt cgcccatcga accgaccgct tcctgaccgt tttcacccag
     1501 tacgcggatc accgaatcca gctcttccgc gctgtagtta aattgtttct ggtagctggc
     1561 aagcgtgtcg tcgtccagtt cgcggctacc cacttcttca tcgggcagat cttcaaacgg
     1621 taccagacgg cggacgtttt tctccatcca ctctttataa gggtggcggc ttttcagatc
     1681 gtcatcggtt tctgccgagt gcagaatacg cccactgcgg gtgtcgataa ccatcagttc
     1741 gcctggcccc acgcggcctt tttcgaccac ttcgtcaggc tgataatccc agataccgac
     1801 ttcagaggcg caggtaatga gcttatcttt ggtgatgacg tagcgcgccg gacgcagacc
     1861 gttacggtcg aggttacagg cggcaaaacg accgtcagac atcacgatgc ctgccgggcc
     1921 atcccacggc tccatatgca tggagttaaa gtcaaagaag gcacgcagtt ccgggtccat
     1981 atccgggttg ttctgccagg caggtggcac taacagacgc atggcacgga tgatatccat
     2041 cccgcctgcc agcaggagtt ccagcatgtt atccatcgaa ctggagtcag agccggtttc
     2101 gttgacgaac ggcgcggcgt cgtgcaggtc agggatcagc ggcgtctgga atttataggt
     2161 acgcgcacgc gcccattggc ggttaccggt gatggtgttg atttcaccgt tatgcgcgag
     2221 gtagcggaac ggctgcgcca gcggccagcg cggtacggtg ttagtggaaa agcgctggtg
     2281 aaacaggcag atggccgatt ccagacgcag gtcagcgagg tccaggtaga agcgcggcag
     2341 atccgccggc atacacagac ctttataaat attgaccaga ttcgacaggc tacagacgta
     2401 gaagtctttg tcggcttcga gacgcttttc aatgcggcgg cgggcgataa acagacggcg
     2461 ctccatatcg cgcggacgcc agcctgccgg ggcgttcaca aaaatttgct caatgcgtgg
     2521 cagagaggag agggcgattt cacccagcac gccttcgtta gtggggacat cacgccagcc
     2581 cacaatcgac aacgtttcgc gttgcagctc ttcttcaacg atgcggcgtg cggcagcggc
     2641 gagttcagga tctttattca ggaagagcat cccgacggcg tagttttttg ctaaacgcca
     2701 gccgcgctcc tgcgcaacga tgcgaaaaaa acgatccggt ttttgtaaca gcaagccgca
     2761 accgtcaccg gttttaccat cggcgagaat cgcgccacgg tgctgcatgc gggccagtgc
     2821 gtgt
//
LOCUS       DAWDRC010000532         2824 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13512_length_2824_cov_1.803178, whole genome shotgun sequence.
ACCESSION   DAWDRC010000532 DAWDRC010000000
VERSION     DAWDRC010000532.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13512_length_2824_cov_1.803178"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1733..1742
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          764 a          681 c          681 g          688 t
ORIGIN      
        1 agtaaaagaa taataatcta attacatctt gataactcag ttgaaaatat tattcaacat
       61 acagcttttt tatggtgagc tggctcacat ctccctgact ctgcggcaat atggcgtctt
      121 tagtatctcc tgctgaacgc cctttttatc cagcataaaa ttccgttcag aagcggatta
      181 gtggcactct gataataaga aatcgcaaag aaaaaaattc tccggcgacg cggtttttaa
      241 caggtgttct atgcttgaaa tgaggtgctc cacacagaga gcgccgacaa caatgaggtg
      301 cgcgtatggc gacgatcgat tctatgaata aggacaccac acgtttgagc gatggacccg
      361 actggacgtt cgacctgctg gatgtttatc tggcagagat agaccgggtg gcgaaactct
      421 accggctgga tacctacccg caccagattg aagtgataac ctcagaacag atgatggatg
      481 cctactccag cgtcggcatg ccaattaact atccgcactg gtcattcggt aaaaagttta
      541 tcgagactga acggctgtat aagcacggtc agcaaggact ggcctatgaa atcgtcatta
      601 actctaaccc gtgtatcgct tacctgatgg aagagaacac cattaccatg caagcgctgg
      661 tgatggctca tgcctgctat gggcataact ctttcttcaa aaacaattac ttattccgta
      721 gctggaccga cgccagttcg attgtcgatt atctgatttt tgcccgtaaa tatattaccg
      781 agtgcgaaga gcgttatggc gttgatgaag tagaacggct tctggactcg tgccacgcgc
      841 tgatgaacta cggcgtggac cggtacaaac gcccgcaaaa aatctcgctg caagaagaga
      901 aagcccggca gaagagtcgc gaagagtatc tgcaaagtca ggtcaatatg ctctggcgta
      961 ccctgccgaa gcgcgaggaa gagaaaacgg ttgctgaagc gcgccgctat ccgtccgaac
     1021 cacaagaaaa cctgctctat tttatggaga aaaatgcgcc actgctggaa tcatggcagc
     1081 gtgaaatcct gcgtattgtg cgtaaggtga gccagtattt ttatccgcaa aaacagactc
     1141 aggtgatgaa cgaaggctgg gcgaccttct ggcactacac catccttaac catctgtatg
     1201 atgaagggaa agtaacggaa cgttttatgc tggagttttt gcacagccac accaatgtgg
     1261 tcttccagcc gccctataac agcccgtggt acagcggcat caacccgtat gccctcgggt
     1321 ttgccatgtt ccaggatatt aaacggattt gtcagtcacc aacggaagaa gacaaatact
     1381 ggttcccgga tatcgccggt tccgactggc tggaaacgct gcatttcgcg atgcgtgatt
     1441 tcaaagatga gagttttatc agccagttcc tgtcaccgaa agtgatgcgt gatttccgct
     1501 tcttcaccgt gctggatgat gatcggcata attatctgga gatttccgct attcataatg
     1561 aagaaggtta tcgggaaatc cgtaaccggt tatcgtcgca atataactta agtaatctgg
     1621 agccgaatat tcagatctgg aacgtggatt tgcgcggcga ccgttcgctg acgctgcgtt
     1681 acattccaca taatcgcgca ccgctggatc gggggcgcaa agaagtgcgg aannnnnnnn
     1741 nnaagacggc agcgtcgagt tgctggaacg ttgcccgccg agaatgggaa atctgtaaat
     1801 agtcctcctc gtttgaggag gactcatttt tgccggatgc ggcgtaaatg ccttatccgg
     1861 cctacaaagt atcgcaaatt caatacattg caaaaaaaca cgtaggcctg ataagcgtag
     1921 cgcatcaggc aattatacgt ttgtcatccg tctggaagga ttatcgtccc tgaatggcta
     1981 aatcacccgg cagatttttc tgcatccggt gccaaatctc gccactctca tgcccatagc
     2041 gacgcactgt ttcatatacc tgatcgtgtg cgccttcact gcacaacgcc gacagtttgt
     2101 gatagaagcc cagcgccaga ctgcgcgctt ccggattggc gaaatagtga cgaccaatgc
     2161 gcgtatacag ccctttcatc ccgttaagga tcagaccgta aatcgggttg ccggaagcaa
     2221 acgccaggcc gcggaaaatg ttgtaatcca gctcggcaaa ggcgtcggcg tgatcggcca
     2281 cttcattagc ggttgccagc acttcctgcg ctttatcggg atgttgacga aacgcggtgc
     2341 gaataaaaat agttgaaata ttggtacgca ccgacagcaa attatcaata agttgcggca
     2401 cactttcgtg gtccagtcgc gccagtgttt caaggatatt taaaccagag gtttcccaga
     2461 aattattcac cttcgtcggc ttgccgtgtt gaatggtcaa ccagccatct cgtgccagac
     2521 gttgtaacac ttcacgtaac gtagtacgcg ttacgccaat taattctgaa agttcacgtt
     2581 ctgcgggcaa aatagtcccg ggagggaagc ggttattcca gatactttca ataatgtact
     2641 cttccgcgaa acccgccggg ctttgcgcct taatgaccat agtgagattt ccataacaca
     2701 gcaaaacaaa gttggactca tcataccaga gggctaacta cgctgataga agacaaaaag
     2761 aagaaaaaag ggatcaaggc ttcattttct gcatctcttt ccccttgact atcaagactt
     2821 gcct
//
LOCUS       DAWDRC010000533         2816 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13547_length_2816_cov_2.203549, whole genome shotgun sequence.
ACCESSION   DAWDRC010000533 DAWDRC010000000
VERSION     DAWDRC010000533.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13547_length_2816_cov_2.203549"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2072..2081
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          744 a          670 c          641 g          751 t
ORIGIN      
        1 gcgtagtggc gatacgttgt gccgcgcccg tatcagaaat cagcttgcct aacatggccc
       61 cgaaaccgag gatcattgcc agcccgccga gtgtgctgcc gataccattt tgtatagagt
      121 gcaggacggc ctgtgcatcc atcccttcgg caaatccgac gacggcagcg accagaacga
      181 gggcaataaa gccgttaact ttaaagccga tcatcaggat aagaagcagc gcgacgcctg
      241 ccgcaataat gattaatggc atagtcttta tctcttatta gtgccagaaa agcagcgctt
      301 tcgcgctgca cgtagggtag tagctgttaa acagccacta acatgccgcc atcaacaaac
      361 aacaggtggc cgtttacgaa atcagaggct tttgaagaaa ggaacaccgc agcaccaatc
      421 agttcctgcg gatctcccca gcgtgcggcg ggggtccgtt tgcacaacca ggcggtgaag
      481 gcttcgtcct caaccagtgc tttagtcatt tctgttttga aatagcccgg cgcaataccg
      541 ttgacctgaa tattgtggcg cgccagctcg acgcacatgc cgcgggtgag catttttacc
      601 gcccctttcg atgcggcata aggggtgatg gtgtcacgtc ccagttcgct ttgcatcgag
      661 caaatattaa taactttacc tgccttgcgt tcaaccatgt gacgagttac cgcttgcgac
      721 accaggaaca cggcggtctg gtttactgcg atcacatcat tccactcttg ttcagggaac
      781 tcagtaaaag gatgacggcg ctggataccg gcgttattca ccagcacatc aatggggccg
      841 atgtcctttt cgatatgttc aacggcggca tcaatttcat gtttatgagt aacattaaaa
      901 ggtgcggcaa cggcctgaat accctcctgg tggagttttt ctacagcaag ttctgcgcgt
      961 tcggcagtaa tatcattaat aattatttgt gcgccatatt tacccaggcc ggttgccagt
     1021 aaaaagccaa tgccctgtgc tgaaccggta atcaagatat tttttcctgc cagtgaaaat
     1081 agatcgttca ttgtattatt cctttattta tctcttagaa aacaagctgg acttttgctg
     1141 cctgggtttt atcaccggcg aaacgtagcg cctcttccag gtcagtgaag ggatattcag
     1201 cactcagtaa aggcagtgga ttaataacgc cattcgccag ccatgacact gcggtattaa
     1261 attcgctggt aaaacggaaa gagcctttga gtgaaatctc cttaccaatc aatgtcatca
     1321 ttgggaattc tgccatcgcg cctcccatac ctacctgcac cattacgccg cgtgcacgag
     1381 tgacctccag acaggtattc actgatgaag gatgaccgga cacttcaaag ctgacatcga
     1441 aatagccttt ttccgctttc cagtgatcca tgtcgtcgtt ttgtgggttt acgagcacat
     1501 ccgcccccat ctctttgccc agcgaaaggg aacggggact cacatcggca cagacaattt
     1561 ccgcggcccc cagtgttttc actgcactga caatcaggca gccaatgggc ccaacaccag
     1621 aaataaatac tcgcttgccc tgtaactcgc cggcctgatg tgcggcatga atcgcgacgg
     1681 ctaaaggttc ggcaaaagct ataacctttt cgtcagcttt ggccggataa gggacacatt
     1741 gcgacgtttc gaccatttta taacgggtaa agccaccatc aacatgaggg aaatacatgg
     1801 cactgccaaa aaaacgcatc tctgtacact gattctcgtt atgttcaatg cagtatttgc
     1861 agtgaccgca cggtttagac ggattaatgg ctaccgtttg cccttcatgt aattttgatg
     1921 agtcgctatg aataacttta ccgataactt catgacctaa caccatcggt gcctttatca
     1981 tgaaattacc tacttttcct tcctgataat aatgtaaatc ggaaccgcaa attccacctc
     2041 gggttatttg tactaatgtt ccattattat tnnnnnnnnn ngctcggtaa cggcaacagt
     2101 tttcttgccc gcaacaacgc aggactgtgt tttcacttgc ataaattcct cactggtcag
     2161 gtagacacct cggaagcatt taagcggttt taattgtcat tatttgtgat gaagatcacg
     2221 tcagaaaatt gttacattac tatgttacgc ataacgtgat gtgccttgta attcttatca
     2281 acagaaataa aaaaacgtga aattattatg ccgccaggcg tagtatcgcc gcaggtaaga
     2341 tgattcagga gattttaaat ggcgggtgaa agctttattt tgatgggcgt atcagggagt
     2401 ggtaaaacat taattggtag caaggttgcc gcgttattgt ctgcgaaatt tattgatggt
     2461 gacgatcttc atccagccaa aaatatagat aaaatgtcgc aaggcattcc attatctgat
     2521 gaagatcgac ttccctggct ggaacgctta aatgatgctt catacagtct ttataaaaag
     2581 aatgaaacag gatttattgt ctgttcatca ttaaaaaaac agtatcgtga tattttacgc
     2641 aagggtagcc cccatgttca tttcctctgg ttagatggcg attatgaaac tattctcgcg
     2701 cgaatgcagc gtcgggctgg gcattttatg ccggtagcgt tactaaaaag tcagtttgag
     2761 gcgctggagc gtccacaagc agatgaacag gatattgtgc gcattgatat caacca
//
LOCUS       DAWDRC010000534         2815 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13551_length_2815_cov_2.771014, whole genome shotgun sequence.
ACCESSION   DAWDRC010000534 DAWDRC010000000
VERSION     DAWDRC010000534.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13551_length_2815_cov_2.771014"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    668..677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          834 a          608 c          644 g          719 t
ORIGIN      
        1 ttttatactc aggtatttaa tctggttctc tgtttattta aataatgtgg aaagagaatt
       61 ttcacaggag accttataca aaaaatataa aatacagcta ctcgttgcca aagacactat
      121 aagcctggca aaaaatatta cacaacataa atgctaattg tttatgcggg ctttgtattg
      181 ctttctgtat cctacaaatg agtgaaatgt atgaaaaagg ctaaaatact ttctggcgta
      241 ttattactgt gcttttcgtc accactaatt tctcaggctg cgacactgga cgtacgtggt
      301 ggatatcgta gtggaagcca cgcctatgag actcgactca aagtcagtga gggatggcaa
      361 aatggatggt gggcaagtat ggaaagtaat acctggaata ccattcatga taataaaaag
      421 gaaaatgccg cactcaatga tgttcaggtt gaagttaatt acgcgattaa acttgatgat
      481 caatggacgg tgcgcccggg aatgttaacg cattttagca gcaacggcac acgctacgga
      541 ccctacgtaa aactgtcctg ggacgcgaca aaagatctta attttggcat tcgctatcgt
      601 tacgactgga aagcttaccg acaacaagac ttatccggtg atatgtctcg tgataccgtt
      661 cctcgttnnn nnnnnnngat ttcaccttcg catggcaaac gccgctatac agcaaacaga
      721 acgattatcg ctatgcaaac cataagaaat gggcgacgga aaatgcattt gttctacaat
      781 accatatgac gcccgatatt acgccataca tagaatatga ctaccttgac cgtcagggtg
      841 tttacaacgg cagagataat ttatcggaaa acagttatcg cattggtgtg tcatttaaac
      901 tgtagtagac aggagacagt cacaatgaat aaaacaataa cggcgcttac tatcataatg
      961 gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc ctgtgccatt taaaagtggt
     1021 accggagcaa ttgataacga cactgtctac attggtttag gtagcgcagg tacggcatgg
     1081 tacaagctgg atacacaggc caaagataaa aaatggacgg cgttagctgc attccctggc
     1141 ggaccgagag atcaagcaac ctctgcattt attgatggca atctgtatgt gtttggcggc
     1201 attggcaaaa acagcgaggg cttgactcag gtatttaatg acgtacacaa atacaacccc
     1261 aaaaccaata gctgggttaa attgatgtcg cacgccccga tgggcatggc gggccatgtg
     1321 acttttgtac acaacggcaa ggcttatgtt actggcggtg ttaaccagaa tatcttcaat
     1381 ggctattttg aagatctcaa ccaggctgga aaagattcaa ccgctataga taaaatcaac
     1441 acccactatt ttgacaaaaa agcagaagat tatttcttca ataaatttct gttgtctttt
     1501 gatccctcaa cacagcaatg gagttacgct ggcgaatctc cctggtacgg aacggctggt
     1561 gcggcggttg tgaataaagg tgataaaacc tggcttatta atggcgaagc caaacccgga
     1621 ttgcgaacgg atgccgtatt tgaacttgac ttcaccggta ataatttaaa atggaataag
     1681 cttgctcccg tcgcatcacc agatggcgtc gctggcggct ttgcaggcat gagcaatgac
     1741 tcactgatat ttgccggagg ggccggattc aaaggttcac gagaaaatta ccagaacggt
     1801 aagaactatg cgcatgaagg cctgaaaaaa tcatatagcg ctgatattca tctttggcat
     1861 aacgggaaat gggataaatc gggcgaatta tcgcaaggtc gggcctacgg agtatcattg
     1921 ccctggaata atagtgtatt aattattggc ggtgaaactg caggcggcaa agcggtgacg
     1981 gattcagttt tgatttctgt gaaggataat aaagtcacag tacaaaacta acgcttcagg
     2041 gccccggtaa aggggccctg ctatcaattt gtcatttgta ttacgcgaaa attatatgaa
     2101 cgcaataata tcgcccgatt attactatgt tcttaccgtt gctggtcagt ctaatgccat
     2161 ggcgtatggc gaaggactac cattaccgga caaggaagat gcgcctcatc ccagaattaa
     2221 acaattagcg agatttgcgc atacgcatcc cggaggcccg tcatgtcact ttaacgacat
     2281 tattccactg actcactgcc cacacgatgt tcaggatatg cagggttatc accatcctct
     2341 ggcaacgaat catcaaacac agtacggcac cgttggccag gcactgcata tagcacggaa
     2401 attactgccc tttattcctg ataatgcagg ggttctcatc gttccgtgtt gccgtggcgg
     2461 atcagctttt accgcgggca gcgaagggac atattcagaa ccgcacggag ccagccatga
     2521 tgcttgtcgt tggggatcgg atactccgct ataccaggat ttagtcagca gaacgcgagc
     2581 cgcactggca aaaaatccgc agaacaaatt cctcggcgta tgctggatgc aaggcgaatt
     2641 tgacttaatg accagtgact acgcgtcaca ccctcaacac tttaatcata tggttgacgc
     2701 ctttcgtagg gctctaaaac aataccattc tcagcttaat aaaattactg acgcgccgtg
     2761 gttttgcggc gataccacct ggtactggaa agaaaatttc cctcatgcgt atgaa
//
LOCUS       DAWDRC010000535         2813 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13568_length_2813_cov_2.838289, whole genome shotgun sequence.
ACCESSION   DAWDRC010000535 DAWDRC010000000
VERSION     DAWDRC010000535.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13568_length_2813_cov_2.838289"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          721 a          652 c          730 g          710 t
ORIGIN      
        1 tctcttacga gcagagccgt attcgtagcg ttgacgtagg cacctggatt gccggtgttg
       61 gttaccgctt ctaatcactt cggtgatata aaaaatccgc ctctcggggc ggattttttt
      121 gttttaaggt ttcgggtcga aaatatcggt tccgagatgg ttgtaatcca ccttctgtaa
      181 ctggaagttg gtgatgtata tcggcccggc tttcttctca gatataaacg ggtatttgat
      241 atttatttcc ttcgctttaa ttcccgtcca ctgcgagaaa aagcctaaga agtcattggc
      301 cgagcggcgg gctttaatca cccgatgcgc tttatcgtcg ctggaaatga ccataaaagg
      361 cacctggaaa ttttgctgat acttatcatc atgggcaagg tattgcacgt ctttgccgcg
      421 ctctttaaag gccagaccgt ggtcagaaaa gtaaaccagc gagaagctgc tgccgctgtt
      481 gcgtaactga tcgtacagct tgcgcagtaa atcgtctgtt tgcgtcatgg tatagagata
      541 gcacgacgtt tctttcgatt gcacaaaggt ttcgtatttg ccttgtgtcc ggtcgcaggc
      601 ctgcggatgc gagcccatca gatgtagaac aatcagctgc ggttgcgagt gctcttgcgc
      661 cagcacttga gcggtcatat ccagtaacgc ttcgtctttc gtgtttttat ctgcttcaaa
      721 attaccttct ttcaggaagt acacttcatc tgctcgtttg gcgatgctgg cgatggcggt
      781 atcgtattcg ccgatttggc cctggttgga aaaccaccag gtctggaagc ccgcgcgatt
      841 tgccagggtg acaaagttat cctgaaactg tggtttgcca tcgacaacgc gattgagcgt
      901 taagccaagc gatttctgcg tggagccact ggcggcaatg tagtcagcaa atatcaaacc
      961 gttaacgctg ctggcaaacg gggtattgtc ccagtgaccg ccaaaggcac cgagggcatc
     1021 gcgacgcgcg ctttctccga tcaccaccac ataggtctga tacttcggct taacggcagt
     1081 gaccgtccag gtatctttca ttccggaaag ttttgccatc cgttcttgct cttcaatcac
     1141 ctcgttatta ttgacgatga catccttagc aaaacgaaat accggatagc cagtatcttt
     1201 cagtttaaat acgccgcccc aggccaggtt ttgcacggga gcaacaaaaa atgtcgccgc
     1261 actgaatacc aggcacaggc tgtcgaaggt attccagcgc gctttttcac tctctttttt
     1321 tcgcctgatt gctattacgc cgagcacaaa aataaatatg ccgaccaggt agctgtacca
     1381 cggaaaaatc gtcaggattt cggtcgactc ttccatattg gtggagtgca atgccagcaa
     1441 tgtattgaag ttaggcgcgc cgtaggcctg agcaaaaggg aaataacaag ccgtcaccag
     1501 cgaactgata ccgaccagaa ctttttgtac gcgaggcaat gttcgccata gcaaaagcaa
     1561 aatagccgta aacgcagcgg tgtagagcaa actgaagggg taacctaagc cgaggttaat
     1621 taatagcgac tgtaaaaagt agaacgcagt ccacgggcta aatacccggc tacgggtaac
     1681 aagcgattct ttgagggtta aattcatatg ccactatcgt aacaaacgcc atgtgcttac
     1741 cctggcgtca agggtcaata cctgcaagag tgcgtgaaga ggggaaaggg gtccgcatcc
     1801 gcgagccgca atatacgcag ggctgcaaga agatagagcg agctgctgtt caggtcaact
     1861 accgacgaac aaatcttttg cgaaggggat tgcaaatccg acaaaaagtg gggtttttca
     1921 gaccaaaacg cgttgtgcct gcataaaagc aggagtgaag cggcgtaccg cgacgccgct
     1981 atttatcgga aggtttatcc tgctgcggtt tgttgttgac catatcgcac aacatagaga
     2041 gcagcattaa ccgtacttta aagggagaat gactaaacac gcgcatacac ctcttgaact
     2101 cattcataag acctcctgac ttgctaatcc catcgatcct tgagggatga ttgcattaca
     2161 tacagatata gcacaggcta tattatatag ctattgctaa aacgttaatt ttttgtacct
     2221 tcgcaactct ggtttacaat gtgcgcacga aatgagaacg ctatgtatgc gtcaccataa
     2281 tgaggaagca caatgagtcg tcgcgcaggt acgccaacag caaaaaaagt gacgcagtta
     2341 gtgaacgtgg aagagcacgt tgaagggttc cgccaggtca gagaggcgca tcggcgcgag
     2401 ctgattgatg attacgttga gctgatttct gacttgatca gggaagtggg ggaagctcgt
     2461 caggtagaca tggctgctcg tctgggggtt tcgcaaccga cggtggctaa aatgcttaag
     2521 cgactggcaa ccatggggct gattgaaatg atcccctggc gtggcgtgtt tttaacggca
     2581 gaaggagaga agctggcgca ggaaagccgc gagcgacatc agatagtcga aaatttcttg
     2641 ttggtgttgg gcgtcagtcc ggaaatcgcc cgtcgcgacg cggaaggtat ggagcaccat
     2701 gttagtgaag agacgctgga tgcctttcgt ttgtttaccc agaaacacgg tgccaaatga
     2761 gcctgccttt tttacgcacg ctgcaaggcg atcgtttttt gcagttatta att
//
LOCUS       DAWDRC010000536         2804 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13630_length_2804_cov_1.846126, whole genome shotgun sequence.
ACCESSION   DAWDRC010000536 DAWDRC010000000
VERSION     DAWDRC010000536.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13630_length_2804_cov_1.846126"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          654 a          617 c          769 g          764 t
ORIGIN      
        1 aactctcctg gcgcaccatc gtcggctaca gcctcggtga cgtcgccaat aacttcgcct
       61 tcgcaatggg ggcgctcttc ctgttgagtt actacaccga cgtcgctggc gtcggtgccg
      121 ctgcggcggg caccatgctg ttactggtgc gggtattcga tgccttcgcc gatgtctttg
      181 ccggacgagt ggtggacagt gtgaataccc gatggggaaa attccgcccg tttttactct
      241 tcggtactgc gccgttaatg atcttcagcg tgctggtatt ctgggtgccg accgactgga
      301 gccatagcag caaagttgtg tatgcatatt tgacctacat gggactcggg ctttgctaca
      361 gcctggtgaa tattccttat ggttcacttg ctaccgcgat gacccaacaa ccacaatccc
      421 gcgcccgtct gggcgcggct cgcgggattg ccgcttcatt gacctttgtc tgcctggcat
      481 ttctgatagg accgagcatt aagaactcca gcccggaaga gatggtgtcg gtatatcatt
      541 tctggacaat tgtgctggcg attgccggaa tggtgcttta cttcatctgc ttcaaatcga
      601 cgcgtgagaa tgtggtacgt atcgtggcgc agccgtcatt gaagatcagt ctgcaaaccc
      661 tgaaacggaa tcgcccgctg tttatgttgt gcatcggtgc gctgtgtgtg ctgatttcga
      721 ccttcgcggt cagcgcctcg tcgttgttct acgtgcgcta tgtgttaaat gataccgggc
      781 tgttcactgt gctggtactg gtgcaaaacc tggtcggtac tgtggcatcg gcaccgctgg
      841 tgccggggat ggtcgcgagg atcggtaaaa agaatacctt cctgattggc gctttgctgg
      901 gaacctgcgg ttatctgctg ttcttctggg tttccgtctg gtcactgccg gtggcgttgg
      961 ttgcgttggc catcgcatca attggccagg gcgttaccat gaccgtgatg tgggcactgg
     1021 aagctgatac cgtagaatac ggtgaatacc tgactggcgt gcgaattgaa gggctcacct
     1081 attcactatt ctcatttacc cgtaaatgcg gtcaggcaat cggaggttca attcctgcct
     1141 ttattttggg attaagcgga tatatcgcca atcaggcgca aacgccggaa gttattatgg
     1201 gcatccgcac atcaattgcc ttagtgcctt gcggatttat gctactggca ttcgtcatta
     1261 tctggtttta tccgctcacg gataaaaaat tcaaagaaat cgttgttgaa attgataatc
     1321 gtaaaaaagt gcagcagcaa ttaatcaacg atatcactag ttaatattca ataaaaataa
     1381 tcagaacatc aaaggtgcaa ctatgagaaa aatagtggcc atggccgtta tttgcctgac
     1441 ggctgcctct ggccttacct ctgcttacgc ggcgcaactg gctgacgatg aagcgggact
     1501 acgcatcaga ctgaaaaatg aattacgcag ggctgataag cccagtgctg gcgcggggag
     1561 agatatttac gcatgggtac agggaggatt gctcgatttc aatagtggtt attattccaa
     1621 tattattggc gttgaaggcg gggcgtatta tgtttataaa ttaggtgctc gtgctgatat
     1681 gagcacccgg tggtatcttg atggtgataa aagttttggc tttaccctgg gggcagtaaa
     1741 aataaaaccc agtgaaagta gtctgcttaa attaggtcgc ttcgggacgg attatagtta
     1801 tggtagctta ccttatcgta ttccgttaat ggctggcagt tcgcaacgta cattaccgac
     1861 agtttctgaa ggagcattag gttattgggc tttaacgcca aatattgacc tgtggggaat
     1921 gtggcgttca cgagtatttt tatggaccga ttcaacaacc ggtattcgtg atgaaggggt
     1981 gtataacagc cagacgggaa aatacgataa acatcgcgca cgttcttttt tagccgccag
     2041 ttggcatgat gataccagtc gctattctct gggggcatcg gtacagaaag atgtttccaa
     2101 tcagatacaa agtattctcg agaaaagcat accgcttgac ccgaattata cgttgaaagg
     2161 ggagttgctc ggcttttacg cgcaacttga aggtttaagt cgtaatacca gccagcccaa
     2221 tgaaacggcg ttggttagtg gacaattgac ctggattgcg ccgtggggaa gcgtatttgg
     2281 cagtggtggt tatttgcgcc atgcaatgaa tggtgccgtg gtggataccg acattggcta
     2341 tcccttttca ttaagtcttg atcgtaaccg tgaaggaatg cagtcctggc aattgggcgt
     2401 taactatcgt ttaactccgc aatttacgct gacatttgca ccgattgtga ctcgcggcta
     2461 tgaatccagt aaacgagatg tgcggattga aggcgcgggt atcttaggtg gtatgaacta
     2521 tcgggtcagc gaagggccgt tacaagggat gaatttcttt cttgctgccg ataaagggcg
     2581 ggaaaagcgc gatggcagtg cgctgggcga tcgcctgaat tactgggatg tgaaaatgag
     2641 tattcagtat gactttatgc tgaagtaaga aaaaacgccg gagagaaaaa tctccggcgt
     2701 tttggctgtg aattactgct gcggaatagc gggtacagcc ggaacgttta atgccggcat
     2761 cgcaaacata cccacgaaat cttccagtgg cattttctgc ccgt
//
LOCUS       DAWDRC010000537         2801 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13644_length_2801_cov_2.856154, whole genome shotgun sequence.
ACCESSION   DAWDRC010000537 DAWDRC010000000
VERSION     DAWDRC010000537.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13644_length_2801_cov_2.856154"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          596 a          735 c          762 g          708 t
ORIGIN      
        1 atataaaaaa gtacatttat ctcatttaaa tatttagatg aggatgtggc aaacgtttgc
       61 ttttctcttt cggtcagcta aaatgcccgc tttgctacca cgggattgtt ttcgatgtct
      121 gtttccaccc tcgagtcaga aaatgcgcaa ccggttgcgc agactcaaaa cagcgaactg
      181 atttaccgtc ttgaagatcg tccgccgctt cctcaaaccc tgtttgccgc ctgtcagcat
      241 ttgctggcga tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt
      301 ttaccggcac aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca
      361 tcgattattc aaattaaggc ctggggtccg gttggctccg ggttgttgtc tattcagggc
      421 accagcttca actttgttgc tccgctgatt atgggcggca cagcgctgaa gaccggtggt
      481 gctgatgttc ctaccatgat ggcagctttg ttcggcacgt tgatgctggc aagttgtacc
      541 gagatggtga tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctggtttct
      601 ggcgttgtgg tgatgattat cggcctgtcg ctaattcagg ttggactgac ctccattggc
      661 ggcggttacg cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca
      721 ggcgtggtct tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg
      781 gcctcactgg tgattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg
      841 ccagaaagca acgaaccgat gacacaagaa ctgattatgg tgccaacgcc gctctattac
      901 ggtcttggca ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg
      961 ctggaaacca ttggcgatat cacggcgacc tctgacgttt ccgaacagcc ggtttccggt
     1021 ccgctgtaca tgaaacgcct gaaaggcggc gtgctggcaa acggcctgaa ctcgtttgtc
     1081 tcggcggtat ttaatacttt cccgaactcc tgcttcgggc agaacaacgg ggtgatccag
     1141 ttaaccggtg ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg
     1201 ggtctgttcc cggcagtgag cggttttgta caacacattc cagaaccggt tctgggcggc
     1261 gcaacgcttg taatgtttgg caccatcgcc gcctccggtg tgcgtatcgt ttctcgtgag
     1321 ccgctgaacc gccgggcgat tctgattatc gcgctgtcgc tggcggtcgg tctgggcgtg
     1381 tctcagcagc cgctgatttt gcagtttgcc cctgaatggc tgaaaaacct actctcctcc
     1441 gggattgccg cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa
     1501 cagtaattca ctggcggcgg taagataatt accgccgcag tctcacctct ttcaccattc
     1561 ccgccttgag gattgcgcgt aaatcgtgca taactccctt atgtgcattt cacgggatgg
     1621 aagaccatga aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggtg
     1681 atcgctggcc tctattttct tctgcaaact cgctggggag cagaacatat cagcgcatgg
     1741 gtttccgaga atagcgacta tcatctggcc ttcggggcga tggatcaccg tttttccgcg
     1801 ccatctcata tcgtgctgga gaacgtcacg tttggccgtg atggccagcc cgcgaccctg
     1861 gtggcaaaaa gtgtcgacat tgcgctaagc agtcggcaac tgaccgaacc acgccatgtc
     1921 gataccatcc tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg
     1981 ttcaaagccg atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg
     2041 aaactgagcg cgcagcgggt aaatggcggc gtggttccgt ggtcaccaga agccggtaaa
     2101 gtgctgggta cgaaggcgca gattcagttt agtgccggat cgctttcgct caatgatgtt
     2161 cctgccacca atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac
     2221 ctgggtgccg acatcgcccg cgggacatta accggaaacg cgcagcgtaa cgctgacggc
     2281 agctggcaag tggaaaacct gcgcatggcg gatatccgtc tacaaagcga aaaatcgcta
     2341 accgatttct ttgcgccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtc
     2401 atcgatgctc gtttgcaagg tccggactgg gcggtgaccg acctcgatct cagcttgcgc
     2461 aacatgacct tcagtaaaga tgactggcag acacaggaag gcaaactgtc gatgaacgcc
     2521 agcgagttta tttatggctc gctgcattta tttgatccga ttataaacgc agaattttcc
     2581 ccgcagggcg tagcactgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg
     2641 tcaggaaact ggcaacgcga cgggaaaacg ctgatcctcg atgatgcggc aattgccgga
     2701 ctggaatata ccttgcccaa aaactggcaa cagttgtgga tggaaacgac acccggttgg
     2761 ttaaacagcc tgcaactgaa gagatttagc gccagccgca a
//
LOCUS       DAWDRC010000538         2788 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13703_length_2788_cov_2.506403, whole genome shotgun sequence.
ACCESSION   DAWDRC010000538 DAWDRC010000000
VERSION     DAWDRC010000538.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13703_length_2788_cov_2.506403"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          543 a          710 c          822 g          713 t
ORIGIN      
        1 acgcgtctcc tttagttacg atggcaacac ggtcacgctg ccggttgaaa tcgccgaagg
       61 actgacggca gggcaggtgg gcttgccgat gggtatgtcc ggcattgctc cggtgctggc
      121 tggcgcgcat cttgaggatc tcaaggaggc acaacaatga gttggataac accggaactg
      181 attgagatcc tgctgaccat cctcaaagcg gtggtgatcc tgctggtggt tgtcacctgt
      241 ggggcattca tgagctttgg cgaacgtcgc ctgctggggc tgttccagaa ccgttacgga
      301 cctaaccgtg ttggctgggg cggttcgctc cagctggttg cggacatgat caaaatgttc
      361 tttaaagaag actggatccc gaaattctca gatcgcgtca tctttaccct ggcgccgatg
      421 atcgccttta cctcgttgct gctggccttt gcgattgtgc cagtcagccc gggttgggtg
      481 gttgccgacc tgaacatcgg gattttgttc ttcctgatga tggcaggtct ggcggtctac
      541 gcggtgctgt ttgcgggctg gtcaagtaac aacaaatact cactgctggg tcggttgcgt
      601 gcctctgcgc agaccctgag ctacgaagtg ttcctcgggc tttccttgat gggcgtggtg
      661 gcgcaggccg gttcattcaa catgaccgac atcgtcaaca gccaggcgca tgtgtggaac
      721 gtcatcccgc aattctttgg ttttattacc tttgccatcg cgggcgtggc ggtgtgtcac
      781 cgtcacccgt ttgaccagcc ggaagccgag caggaactgg cggatggtta ccacattgaa
      841 tattccggta tgaagttcgg tctgttcttc gtgggcgaat acatcgggat tgtgaccatc
      901 tctgcactga tggtgacgct gttcttcggt ggctggcaag gcccgttgtt accgccattc
      961 atctggttcg cgctgaaaac cgcgttcttt atgatgatgt tcattttgat tcgtgcgtcg
     1021 ttaccgcgtc cgcgttatga ccaggtaatg tccttcggct ggaaaatctg cctgccgctg
     1081 acgctgatca acttgctggt aacggcggct gtcattctct ggcaggcgca ataaggggca
     1141 ataagaccat gaccttaaaa gaattgttag taggtttcgg cacccaggtt cgtagtatct
     1201 ggatgatcgg cctgcacgcg ttcgccaaac gcgaaacgcg aatgtacccg gaagagccgg
     1261 tctatctgcc gccccgttat cgtggtcgta tcgttctgac ccgcgatccg gacggcgaag
     1321 agcgttgcgt agcctgtaac ctctgcgcgg tagcctgccc ggtcggctgt atctcgctgc
     1381 aaaaagcaga aaccaaagac ggtcgctggt atccggaatt tttccgcatc aacttctcac
     1441 gctgcatttt ctgtggtctg tgcgaagaag cctgtccgac cacggcgatt cagttaaccc
     1501 cggatttcga aatgggggaa tacaagcgcc aggatctggt ttacgagaaa gaggatctgc
     1561 tgatctccgg tccgggcaaa tacccggaat ataacttcta ccggatggca ggtatggcaa
     1621 tcgacggcaa agataagggc gaagcagaga acgaagccaa gcctatcgac gtcaagagcc
     1681 tgttaccgta aggagagggg caatggagtt cgctttttat atctgtggcc tgatagccat
     1741 acttgcgacc ttgcgagtga tcacccatac caatccggta cacgcattgc tgtacctgat
     1801 tatttcgctg ctggcgattt ccggggtgtt cttctcactg ggcgcttact tcgccggtgc
     1861 gctggaaatt atcgtctacg cgggggccat tatggtgctg ttcgtgttcg tggtgatgat
     1921 gctcaacctg ggcggttcgg aaatcgaaca ggaacgccag tggctgaaac cgcaggtgtg
     1981 gattggtccg gcaattttgt cagccatcat gctggtggtg attgtttacg ccattctcgg
     2041 tgttaacgat caaggtatcg acggtacgcc aatcagtgct aaagcagtgg gtattacgct
     2101 gttcgggcct tacgtactgg cggtggaact ggcttctatg ctgctactcg cgggtctggt
     2161 tgtggccttc cacgtcggtc gtgaagagcg tgcgggtgaa gtgctgagca accgtaaaga
     2221 cgacagcgcg aaaagaaaaa cggaggagca cgcatgatcc ccttacaaca tggactgatc
     2281 ctcgcggcaa tcttattcgt tcttggctta accggtctgg ttatccgtcg caatctgctg
     2341 tttatgctga ttggtctgga aatcatgatt aacgcctccg cgctggcctt tgtggtcgcc
     2401 ggaagctact ggggccagac cgacggtcag gtgatgtaca ttctcgccat cagcctcgcg
     2461 gcggcggaag cgagtatcgg ccttgcgctg ctgctgcaac ttcaccgtcg tcgccagaac
     2521 ctgaacatcg attcagtaag tgagatgcgc ggatgaacat gcttgcctta accattattt
     2581 tgccattgat tggcttcgtc ctgctggcgt tctcccgtgg gcgctggtct gaaaacgtct
     2641 cggcgatcgt tggcgttggc tctgtgggcc tggcggcgct ggtaactgcc tttatcggcg
     2701 ttgacttctt cgctaacggt gagcaggcat acagccagcc gctgtggacg tggatgtcgg
     2761 taggcgactt taacatcggt tttaacct
//
LOCUS       DAWDRC010000539         2784 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13731_length_2784_cov_1.580066, whole genome shotgun sequence.
ACCESSION   DAWDRC010000539 DAWDRC010000000
VERSION     DAWDRC010000539.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13731_length_2784_cov_1.580066"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    364..373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          599 a          720 c          753 g          702 t
ORIGIN      
        1 cccgtggaag aagacgccta taacaccaaa gtcattactc tttcacgtag cggttcgctg
       61 ccggaagtga tcgaaaccag ccatgactac gccaaagtga tggacaaaga gcagcttatc
      121 gatcgccagg cggttgccac ggtcatcagc aacgtcggtg aaggcgcgtt ctacgatggc
      181 gtactgcgga ttgtgcgcac cgaagatggc agcgcctgga ccggtgttcc tgtcagcgca
      241 tggattggcg ggatctggta tcgcaaagat gtgctggcaa aagctgggct ggaggagcca
      301 aaaaactggc agcaattgct ggacgttgca cagaaactga atgacccggc gaataaaaaa
      361 tacnnnnnnn nnntacagca gaaagcgtgt tgacggaaca gtctttctcc cagtttgcgt
      421 tatccaatca ggctaacgtc tttaacgccg aaggcaaaat cacccttgat acaccagaga
      481 tgatgcaggc gctgacctat taccgcaacc ttgctgccaa caccatgcca ggttctaacg
      541 acatcatgga agtgaaagac gcttttatga acggcaccgc gccgatggcg atttactcca
      601 cctatatcct tccggctgtg attaaagaag gcgatccgaa aaacgttggt ttcgtggtgc
      661 caaccgagaa aaattctgcg gtctacggca tgttgacctc gctgaccatt accgccgggc
      721 aaaagactga agagaccgaa gcggctgaaa aatttgtcac ctttatggag caggcggaca
      781 acattgccga ctgggtgatg atgtcacctg gcgcggcgct gccggtgaac aaagcggtgg
      841 tgactaccgc cacctggaaa gacaacgacg ttcttaaggc gctgggagaa ttgccgaatc
      901 agctaatcag cgaactgcca aatattcagg tctttggcgc agtaggggat aaaaacttta
      961 cccgcatggg cgatgtcacg gggtctggtg tggtgagttc aatggtgcat aacgtcaccg
     1021 tgggtaaagc cgatctccct ggcacgctgc aagcgagcca gaaaaagctg gatgaactga
     1081 tcgaactgca ctaaacccag gacaggaatc cgcaatgaac aggctttttt caggtcgttc
     1141 cgatatgcct tttgcactgc tgcttctcgc gcccagctta ttactgctgg gcggtctggt
     1201 ggcgtggccg atggtgtcga atatcgaaat cagtttttta cgtctgccgc tcaatcccaa
     1261 catcgagtca acctttgttg gggtgagtaa ctatgtgcgc attctctccg atcctggttt
     1321 ctggcactcg ctgtggatga cggtctggta taccgcgctt gtggtggcgg gcagcaccgt
     1381 gctcgggctg gccgtggcga tgtttttcaa ccgcgagttc cgcctgcgca aaaccgcgcg
     1441 ttcgctggtg atcctttcct acgtaacgcc gtctatttcg ctggtgttcg cctggaaata
     1501 catgttcaac aacggttacg gcattgtgaa ctacctcggc gtcgatcttc tgcatctcta
     1561 tgagcaggca ccgctgtggt tcgataaccc gggcagtagt tttgtgctgg tggtgctgtt
     1621 cgctatctgg cgctacttcc cgtatgcctt tatctcgttt ctggcgattt tgcagaccat
     1681 cgacaaatcg ctgtacgaag cggcagagat ggatggcgcg aatgcctggc agcggtttcg
     1741 catcgtcacg ctgcccgcga ttatgccggt cctggcgacg gtggtgaccc tgcgcactat
     1801 ctggatgttc tacatgttcg cggatgttta tctgctgacg accaaggtcg atattctcgg
     1861 tgtctatctc tacaaaaccg cctttgcctt taatgactta gggaaagcgg cggcgatctc
     1921 ggtggtgctt ttcatcatta ttttcgctgt cattctgctg accaggaaac gggtgaacct
     1981 caatggcaac aaataaacgc acactcagtc gaatcggttt ttactgcggg ctggcgctgt
     2041 ttctcatcat cacgctgttt ccattttttg tgatgctgat gacctcgttc aagagcgcaa
     2101 aagaggcgat ctcgctgcat cctacgctgc tgccacagca gtggacgctg gagcattacg
     2161 tcgacatttt taatccggtg attttcccgt ttgtcgacta cttccgtaac agcatggtgg
     2221 tgtcggtggt ttcatccgtg gtggcggtat ttctcggcat tctcggggct tacgcgcttt
     2281 cccgcctgcg ctttaaaggg cggatgacca tcaacgccag cttttacacg gtgtacatgt
     2341 tctccggcat tttgctggtg gtgccgctgt tcaaaatcat caccgcactt ggcatttacg
     2401 acaccgagat ggcgctgatc attaccatgg tgacgcaaac cctgcccacc gccgtgttta
     2461 tgctgaaaag ctacttcgac accatccctg atgagatcga agaagcggcg atgatggatg
     2521 gcctcaatcg tctgcaaatt atcttccgta tcaccgtgcc cctggcgatg tccgggctga
     2581 tatcggtgtt tgtctactgc tttatggtgg cgtggaacga ctatctgttt gcgtcgattt
     2641 tcctctccag cgccagcaat ttcaccttac cagtgggcct gaacgcgctg ttcagtacgc
     2701 ctgattacat ctggggacgg atgatggcgg cctcgctggt gaccgcacta ccggtagtga
     2761 ttatgtatgc gctttcggaa cgtt
//
LOCUS       DAWDRC010000540         2771 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13799_length_2771_cov_2.034978, whole genome shotgun sequence.
ACCESSION   DAWDRC010000540 DAWDRC010000000
VERSION     DAWDRC010000540.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_2771_cov_2.034978"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1318..1327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          686 a          704 c          683 g          688 t
ORIGIN      
        1 gccttaccgc gccattgtcg atacaggcgt tccagatctt cgctgttacc tctggaaaga
       61 atcgcctcgc gaaaacgccg cccattttca cgcgttaatc cgccctgctc aacaaaccac
      121 tggtaaccat catcggccaa catttgcgtc cacagataag cgtaataacc tgcggcatat
      181 ccgccaccaa aaatatgggc gaaataactg ctgcgatagc gtggcggtat agcaggaaga
      241 tccatatttt ccgccaccag cgcccgcaat tcgaaatcat cgacgtcctg cattgcttcg
      301 ttttcttcca ggcaatgcca gcgcatatcg agaagtgcgg cgctaagcag ttcgctcatc
      361 tcataccctt tattgaacag gctggcatta cgcattttct gttgaagttc gtcaggcatt
      421 gcggctctgc tctgataatg ccgggcgtag cgagcgaata cctgcggatg tgttgcccag
      481 tgttcgttga tttgcgacgg aaattcgaca aaatcacgcg gcgtgttggt gccggaaagc
      541 gtggcataac gctggcgggc aaaaaggccg tgcagcgtat gaccaaattc atggaataag
      601 gttattacat catcccagag taacaacgca ggctcaccgg cagcgggttt ctgataattg
      661 cagacgttat aaattaccgg gtgcgtttca ttcagcgttg attgctcaac aaaattgccc
      721 atccatgcac cgccgctttt tgaatcacgg gcgaagaaat caccgtaaaa taacgccagc
      781 cccacgccat tatgatcaaa aatttcccac acacgaacgt caggatggta gacaggaata
      841 tcaaaacgtt cgacaaactt aataccgaag agctgattcg cagtccagaa tacgccttca
      901 ttcaacaccg tgtttaattc gaaataaggc ttgagctgcg cttcatcgag atcgaatttc
      961 tcacgtctta cctgctcggc ataaaatgcc cagtcccacg gctgcgcgct aaacccgccc
     1021 tgctgcttat cgataaccgc ctgtatggag gttaattcat cgctcgcacg ttgacgcgcc
     1081 gctggaacaa tttcccgcat aaagttgagc gctgcttctg gcgtttttgc catctgatcg
     1141 gcgattttcc atgcggcata atgaggaaaa ccaagcagtt tcgcctgctg cgcgcggatc
     1201 tccaccagac gttgaatgat agcgcgggta tcattggcat catttttttc cgctcgcgtc
     1261 cagcccgcag taaacagttt ttcacgcgtc gcgcgatcgc gcagttcagc aagcgctnnn
     1321 nnnnnnngcg agccgcctct gccgccagcg caatctcttg ttcactcatt cctgccagct
     1381 gcgcgatatc gttcacaacc agaccgccgg atttatttgc tgccagtaac cgctggttaa
     1441 actggctggt cagggtcgca gcttctgtat tcagtacttt taattttgct ttatcagctt
     1501 gctcaagttt ggctccggca aggacaaaac gttgatgaat cacctccacc aggcggatgg
     1561 attcactatc aagccgcgcg aataattccc cgttcagata gatatcattc gcaagttccg
     1621 ccagttcagc ggaaaactgt tcatcaagac gctgtaattc atcattggta tgcgccgcag
     1681 tcatcgcaaa aaagacgctg gtaacgcggg taagtaattc tccgctttgt tccagtgcca
     1741 gaatagtatt gttgaaatca ggtgtttgcg ggttaagcgc gatggcagct atttctgccc
     1801 gcttttgctg tattccctca tcgaatgccg ggcgatagtg atggttggca atttgatcaa
     1861 aatggggagc cagatacggc agtgtgcttt gcacaaggaa aggattcatt gttgtcattt
     1921 tcttctcctg aacgcgaggt gttccatagc gtaggcttac tgataacgga gtgcaatctt
     1981 gcaaaccagt attacccgct cttaagcatc acgtgctatg ttattgacac acaaaagcgt
     2041 tgaggaacag tgagatgatc gttttagtaa ctggagcaac ggcaggtttt ggtgaatgca
     2101 ttactcgtcg ttttattcaa caagggcata aagttatcgc tactggccgt cgccaggagc
     2161 ggttacagga gttaaaagac gaactgggag ataatctgta tatcgcccaa ctggacgttc
     2221 gcaaccgcgc cgctattgaa gagatgctgg catcgctccc tgccgagtgg agcaatattg
     2281 atatcctggt aaacaatgcc ggcctggcgt tgggcatgga gcctgcgcat aaagccagca
     2341 ttgaagactg ggaaacgatg attgatacca acaacaaagg cctggtatat atgacgcgcg
     2401 ccgtcttacc gggtatggtt gaacgtaatc atggtcatat tattaacatt ggctcaacgg
     2461 caggtagctg gccgtatgcc ggtggtaacg tttacggtgc gacgaaagcg tttgttcgtc
     2521 aatttagcct gaatctgcgt acggacctgc atggtacggc ggtgcgcgtc accgacatcg
     2581 aaccgggtct ggtgggtggc accgagtttt ccaatgtccg ctttaaaggc gatgacggta
     2641 aagcggaaaa aacctatcaa aataccgttg cattgacacc agaagatgtc agcgaagccg
     2701 tctggtgggt gtcaacgctg cctgctcacg tcaatatcaa tacactggaa atgatgccgg
     2761 ttacccaaag c
//
LOCUS       DAWDRC010000541         2771 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13798_length_2771_cov_2.152798, whole genome shotgun sequence.
ACCESSION   DAWDRC010000541 DAWDRC010000000
VERSION     DAWDRC010000541.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13798_length_2771_cov_2.152798"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          670 a          750 c          827 g          524 t
ORIGIN      
        1 gataatcttc tcgactggac gcagaacaat ctcgctcagg gttctttaaa agaacatttg
       61 cccgcctggc tgtggggcaa gaaagagaac agtaagtggg aagtgctgga cagcaattcg
      121 gcctccgatg gtgatgtctg gatggcctgg tcgttgctgg aggcggggcg tttgtggaaa
      181 gagcagcgtt ataccgacat cggcagcgca ttgctaaaac gtatcgcgcg ggaggaagtg
      241 gtgacggtgc ctgggctggg ctccatgttg ttaccgggca aagtgggttt tgctgaggat
      301 aacagctggc gttttaaccc cagctacctg ccgccgacgc tggcgcagta tttcacccgc
      361 tttggcgcgc cgtggaccac gctgcgcgaa accaatcaac gtttattgct ggaaaccgcc
      421 ccgaaaggct tttcgccaga ctgggtgcgc tatgagaaag acaaaggctg gcagctaaaa
      481 gccgaaaaaa cattgatcag cagctacgac gctatccgcg tttacatgtg ggtaggcatg
      541 atgcctgaca gcgatccgca aaaagcgcgg atgctcaacc ggtttaaacc gatggcgaca
      601 ttcactgaga aaaacggtta tccgccggaa aaagtggatg tggctacggg gaaagcgcag
      661 ggtaaaggac cggtcggttt ttctgccgcc atgctgccct ttttacaaaa ccgcgatgcg
      721 caggccgttc agcgccagcg cgtggccgat aactttcccg gcagcgatgc ctattacaac
      781 tatgtgctga ccctgtttgg acaaggctgg gatcaacacc gtttccgctt ctcgacaaaa
      841 ggtgagttat tacctgactg gggccaggaa tgcgcaaatt cacactaaac atattcacgc
      901 tttccctcgg tctggccgtc atgccgatgg tcgaggcagc accaaccgct cagcaacagt
      961 tgctggagca agttcggtta ggcgaagcga cccatcgtga agacctggtg caacagtcgt
     1021 tgtatcggct ggaacttatt gatccgaata acccggatgt gattgctgcc cgttttcgtt
     1081 ctctgttacg tcagggcgat attgatggcg cacaaaaaca gctcgatcgg ctgtcgcagt
     1141 tagcgccgag ttcaaatgcg tataaatcgt cgcggaccac aatgttactg tctacgccgg
     1201 atggtcgtca ggcactgcaa caggctcgat tgcaggcgac taccggtcat gcggaagagg
     1261 ccgtggcgag ttacaacaag ctgttcaacg gtgcgccgcc ggaaggtgac attgctgtcg
     1321 agtactggag tacggtggcg aaaattccgg ctcgccgtgg tgaagcgatt aatcagctaa
     1381 aacgcattaa tgcggatacg ccgggcaata cgggcctgca aaacaatctg gcgctgccag
     1441 cgttcaggca ctgaaaaaat atctctcgat ctttagcgat ggcgatagcg tggctgctgc
     1501 acaatcgcaa ctggcagaac agcaaaaaca gctggccgac cctgctttcc gcgctcgtgc
     1561 gcaaggttta gcggcggtgg actctggcat ggcgggtaaa gccattcccg aactacaaca
     1621 ggcggtgcgg gcgaacccga aagacagtga ggctctgggg gcgctgggcc aggcgtattc
     1681 ccagaaaggc gatcgcgcca atgcagtggt gaatctggaa aaagccctcg cgctggatcc
     1741 gcacaacagc aataacgaca aatggaacag cctgctgaaa gtaaaccgct actggctggc
     1801 gatccagcag ggcgatgccg cgctgaaagc caataatcct gaccgggcag aacggctgtt
     1861 ccagcaggca cgtaatgtcg ataacaccga cagctatgca gtgcttgggc tgggcgatgt
     1921 ggcgatggcg cgcaaagatt atcccgccgc cgaacgctat taccagcaga ccttgcgtat
     1981 ggacagcggc aacactaacg ccgtgcgcgg gctggcgaat atttaccgcc agcagtcgcc
     2041 agaaaaagcc gaagcgtata tcgcctcgct ctctgccagc cagcggcgca gcattgatga
     2101 tatcgaacgc agcctgcaaa acgaccgtct ggcacagcag gcagaggcac tggaaaacca
     2161 ggggaaatgg gcgcaggcgg cagcacttca gcggcaacga ctggcgctgg accccggcag
     2221 tgtatggatt acttaccgac tttcgcagga tctctggcaa gccggacaac gcagccaggc
     2281 cgatacgtta atgcgcaatc tggcgcagca gaagccgaac gacccggagc aggtttacgc
     2341 ttacgggctg tacctctccg gtcacgacca ggacagagcg gcgctggcgc atatcaacag
     2401 cctgccgcgc gggcagtgga acagcaatat tcaggagctg gttaatcgac tgcaaaacga
     2461 tcaggtgctg gaaaccgcta atcgcctgcg agaaaacggt aaagaggcag aagcggaagc
     2521 gatgctgcgc cagcaaccgc cttcctcgcg tattgacctc acgctggctg actgggcgca
     2581 gcagcgacgt gattacaccg ccgcccgcgc tgcatatcag aatgtcctga cgcgggagcc
     2641 aactaacgcc gatgccattc ttggtctgac ggaagtggat attgctgccg gtgacaaagc
     2701 ggcggcacgt agccagctgg cgaaactgcc tgccattgat aacgccccgc tgaacacaca
     2761 gcggccggtg g
//
LOCUS       DAWDRC010000542         2769 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13810_length_2769_cov_1.907885, whole genome shotgun sequence.
ACCESSION   DAWDRC010000542 DAWDRC010000000
VERSION     DAWDRC010000542.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13810_length_2769_cov_1.907885"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1802..1811
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          616 a          724 c          726 g          693 t
ORIGIN      
        1 ccacagccgt agaggtggtt caagtcgacc actccgtcga tgctcgggta tttcggcagc
       61 aggttgcgtt cgataatttt aaccacgtag ttcacaacgt ctgccatgca gtgcacgctg
      121 gtggtaatac ccaacaggtt tttggtgccc acgctgccgt cggcgttgcg gtaaccttca
      181 aaggtgtaat cttccagcgg cggcagcggc tccggcactt tggtcgcccg aggcaggggg
      241 ttaagcggcg gcgctttcgg cagcgtgacc gtggatttgt cgatccagct gcctttggcg
      301 acatcgcgta ccgcgtaacc gataacttcg ccatagcgaa tgatttcacc attctgagga
      361 aggttgttat cccttttctg gcgtattcgt cattccattc gtatccccgg taccggatgt
      421 ttatatcgta ctgtgccggt gcttgcgtgc tttcctgcag gtctgcaccg atttaacaaa
      481 caaaacttaa cagagtctga aggagtagta tttcgtgcaa ataaccgaag cgttattttc
      541 agagcaggga gacatccggc gttttgttca gcaggcggtg gaccactgga cgcatctgct
      601 ggcagtccac ttcacactcc attcggcaga aggaaatatc aacgggcaac agattcatgc
      661 attctgtact tccttttatg aacgtattac tgagcgtaat cacacgacca gtccatcatc
      721 gccgctggta ttacgctggt tgcgggaaca actacatgga ggagcaacaa tacgatgcct
      781 gttgctgctc agccagacga gcatttgcca tccgcgagcc agtgtgacag gtgatgaagc
      841 atgttcacaa ctggtggatt tactgcaaca gacctggcgg gtgataagtg ctggcggaca
      901 atgtcgggtg gaaagttttt tttcgggttg cccggcctga tacatcagag caatacgttg
      961 ctttaaaaac tgctgttcag tctctgatgc tatgggttat tgcaacaatc atccggtgat
     1021 ggacgggaga aatatcgact cttccgttag tttttacagg ctgataaagt tttgccagct
     1081 gaatctttat acggaatact ctccagtatg cgtaagagaa tttgcttttt ggaggggggc
     1141 gacaaccaga tggtaaacaa aagaatggtt gaatgtggat gaggttcata ttcattgatt
     1201 cagatttttt gaagaatcac cacatgggat gtccatgtgg ttgtataact cagggccagc
     1261 gcttaatgat gatggatgta ttaatgccgc caaaggcaaa gttgttactc tgcaaaaact
     1321 cacaatcaac ctttcgggct tcatgcatga tgtaatccag cgcgccacag ttcgggtctg
     1381 gtttattcag gtttagcgta ggcgcaaacc aaccttcgcg catcatttgc agactcatcc
     1441 aggcttcgag cgcgccacag gcaccaagcg tatgaccaaa ataacttttc agcgaggaga
     1501 ccggcacatt atcaccgtaa atggttgctg tcgccagact ttctgccata tcaccgcgat
     1561 cggtcgccgt accatgcgct gaaatatagc cgatatcttg tgcacttaac ccggcgattt
     1621 tcagcgattg ctccatacaa tactgcatgg tttcgcgctg tggctgggta atatgtgccg
     1681 cgtcgcagtt agtcgcaaag ccgacgattt caccgtaaat ggtcgcgccg cgcgctttgg
     1741 cgtgctccag ttcttcgagg atgagcgtac ctgcgccttc accaatcacg gagagatctc
     1801 gnnnnnnnnn natcaccgtt tgataaccat ggcgaatggc ttcccacgcg tagccgatag
     1861 cctgactacc tgacgtgcag gcgctggagg tcggaatcac ccgcccgcgc agtccaaaaa
     1921 acagcccggt gttaacggca gtagtgtgcg gcatcatctg cacataggtg gtgccagtga
     1981 tgttgttcgt gtgcttttcc gtcagcatgg tggcgaactc gcttaccggg ccggtactgc
     2041 cggtcgatga gccgtatgca atgcccgttt cgccgctggt taatatcgga tcgccaatca
     2101 accctgcctg ctctaacgcc agctcgctgg cacgggtcga catttgcgac acgcggccca
     2161 tcgcacggat acgcttgcgg gtgtagtgct ccggcagcgt gaaatcatca acaggcgcgc
     2221 caagcagggt gtgcaggccg tcgtaaacct gccactccgg cattttgcgc accgcatttt
     2281 catacgccag taagcgtgca gaaacgtcct gccagttttc accaaaggca gtcacgccgc
     2341 ccattcctgt aatcactacg cgacgtgtca taacatccct ccgttgatgg aaataacctg
     2401 gcgggtgacg taacccgcaa tatcagacat taaatagctg gcaagcccgg caacttcttc
     2461 cgcctggccc atgcgtttca tggggatcat cgacattgcc tctttcagag cgctctcttc
     2521 catttcgatc atcccggtat caataagccc tggcgcgatg caattgacgg tgattttgcg
     2581 cttcgcgagt tcgatagcca gtgcttttgt tgcgccgata attccggctt tggcagcgct
     2641 gtagttcacc tggccgcgat tgcccattac gccggaaacc gaggacagcg tgatgatgcg
     2701 cccaccctgg cgtgcgccga tcatcggcat aatgcacggc tgaatgacgt tgtagaaact
     2761 gtcgaggtt
//
LOCUS       DAWDRC010000543         2743 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13946_length_2743_cov_2.054688, whole genome shotgun sequence.
ACCESSION   DAWDRC010000543 DAWDRC010000000
VERSION     DAWDRC010000543.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13946_length_2743_cov_2.054688"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2009..2018
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          614 a          775 c          700 g          644 t
ORIGIN      
        1 ttgatataat cctaaaaaaa tctcataaaa ttaatatatg agataatctt tattcagcag
       61 aagattatta aaggttgctg tattatttag cgataaaaaa agcctgccag atggcaggct
      121 ttttaataac ggcgttatta ctgcaacagc gaaatatccg caacgcgcag gaacagttcg
      181 cgcagttttt ccagcatggt cagacggttg atacgcaatt ctttatcatc aaccatgacc
      241 atcactttat cgaagaaagc atcgaccggt tcacgcagct cagccagttc gaccagcgca
      301 tcctggtaac gaccttccgc aaagtacggc tccagcttgt cacgtagcac cacaacctgc
      361 atagccagct taatttcttc cggctctttc agagtagagg cattcacgcg atcgctcagc
      421 acttcgtcag atttcgccag aatgttagat acacgtttgt tcgccgccgc cagtgctgca
      481 gctgcttcca gggtacggaa gtgcgatacc gctttcatac gggcatcgaa atcagccgga
      541 cgagtcggac gacgcgccag taccgcctgg atggtgtcaa cggtgtaacc ttcgtcctga
      601 taccaggcgc ggaagcgacc gagcataaag tcgataacat catcaaccac gttggcgtta
      661 gtcagcttat cgccatacag acgcactgct tcttcggtca gcgtttgcag atcaaggttg
      721 aggttcttct caacgataat acgcagcacg ccaagtgcgg cacgacgcag cgcaaacggg
      781 tctttgtcgc ctttcggatg ctggccgata ccgaagatac ccgccagggt atccatcttg
      841 tcagcaatcg ccagcgcaca ggctaccggg ttagacggca ggtcatcacc ggcaaagcgc
      901 ggctgatact gctcattcag cgcgacagct acgtcttccg cttcgccatc gtgacgcgcg
      961 tagtgcatcc ccataacgcc ctgggtgtcg gtgaactcga agaccatgtt ggtcatcagg
     1021 tcacacttag acagcaggcc cgcacgggta gcgtggttaa cgtcagcgcc aatctgctca
     1081 gcaatccagc cagccagcgc ctgaatgcgg tcagttttgt cgcgcagcgt ccccaactgt
     1141 tgctggaaca acacggtttg caggcgcggc aggttatctt caagacgttt tttacggtcg
     1201 gtattgaaga agaactcggc atccgccaga cgtggacgaa cgactttctc gttaccggag
     1261 ataatctgct gcggatcttt cgattcgatg ttggcaacga agataaagtt cggcagcagt
     1321 ttgccgtcgt tcgcatacac cgggaagtac ttctggtcac ctttcatggt gtaaaccagc
     1381 gcttcagacg gcaccgcgag gaatttctct tcgaattttg cggtcagcac aaccggccat
     1441 tccaccagcg aagccacttc ttccagcaga ctttcgctta agtcagcgtt accgccaatc
     1501 ttacgtgctg cttcttcagc atcggcttta atcttcgcct tacgttcttc gtaatcggcg
     1561 atgactttcc cgcgctcacg cagaatttcc ggatactgat cggcattgtc gatagtgaat
     1621 tccggctcgc ccataaagcg gtggccgcga atcacgcgat cggactgaat acccagaatg
     1681 gtagccggaa taactttgtc gcccagcagc agggtcacgg tgtgaaccgg acgcacgaag
     1741 tgcacgtcgc ttgcgcccca acgcatcagt ttcgggatcg gcagtttcgc cagcgaagtc
     1801 gcaaccatat tcggcagcag tgcttcggtg ctttcaccct tcacatgggc gcgatacagc
     1861 agccattcgc ctttatcggt agtcagacgc tcagcctggt caacggtaat accgcaacca
     1921 cgcgcccaac cttctgccgc tttgctcggt ttgccttcag cgtcgaacgc ctgggcaatt
     1981 gccgggccgc gtttttctat ttcgcgatnn nnnnnnnnat ccggttgcgc ctccgccagg
     2041 ttagcgactt tcagcgccag acgacgcgga gcagcaaacc attgaacggt gccgtgtgcg
     2101 aggccagcgt tatccagctc cgcagtaaag ttcgcagcaa aggactcagc caggctgcgc
     2161 agtgcttttg gtggcagctc ttcagtgccg atttccacca gaaaagtttt ctcagacata
     2221 gccgcctctt acttatcttt gttgcacatc gggaagccga gggcttcacg ggaagcgtag
     2281 tatgcttctg ccactgcttt ggtcagggtg cgaatgcgca gaatatagcg ctgacgctcg
     2341 gtgacggaga tggctttacg cgcatccagc aggttgaagc tgtgggcggc tttcagaata
     2401 cgctcgtagg ctggcagcgg cagcggattt tccagcgcca gcagttgctg cgcttctttc
     2461 tcgtactgct cgaagcaggt gaacaggaag tccacatccg cgtattcgaa gttgtaagtg
     2521 gactgctcca cttcgttctg atggaacacg tcgccgtagg tggttttacc cagcgggccg
     2581 tcgctccaga ccaggtcgta aacgctgtct acgccctgaa tgtacatggc cagacgttcc
     2641 agaccgtagg tgatctcacc ggtaaccggt ttacattcca gaccaccaac ctgctggaag
     2701 taagtgaact gcgtcacttc catgccgttt agccacactt ccc
//
LOCUS       DAWDRC010000544         2741 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13957_length_2741_cov_2.622859, whole genome shotgun sequence.
ACCESSION   DAWDRC010000544 DAWDRC010000000
VERSION     DAWDRC010000544.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13957_length_2741_cov_2.622859"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          639 a          644 c          702 g          756 t
ORIGIN      
        1 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
       61 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
      121 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
      181 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
      241 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
      301 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
      361 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
      421 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
      481 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
      541 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
      601 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
      661 cggcgcgttt aagcaggtat tagtagatag cggtgtcggg cagtatattt cacacttaat
      721 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
      781 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
      841 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
      901 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
      961 tggtgaaacg ttgcgtacct ggacggtgat ggaaaccctt atttctatta tgggtttgct
     1021 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     1081 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     1141 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     1201 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     1261 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     1321 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     1381 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattaca atcgcgattc
     1441 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     1501 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     1561 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     1621 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     1681 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     1741 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     1801 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     1861 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     1921 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     1981 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     2041 gagcaaaatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     2101 tcacggtaac acgctttagg ttattttctc tgcattacac atattctctt agccggacgt
     2161 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     2221 cgcacgccgg acactgaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     2281 actggcgcaa cggggacggg taaaactgtc acgctgcaaa aactggcaga gtcattgtcg
     2341 gaaataggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     2401 gcaggaacga cctcggaaaa actgctcgca agactgaaaa atatcggtgt caatgactgg
     2461 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     2521 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     2581 gtgcaatctg gcgtgctgaa tatcattttc cgcattgctg acgatcaggg attgttgctg
     2641 ctcgacttta aagatctgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     2701 cagaatcagt acggtaatat ccgtagtgca tcggttggtg c
//
LOCUS       DAWDRC010000545         2740 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13964_length_2740_cov_2.218250, whole genome shotgun sequence.
ACCESSION   DAWDRC010000545 DAWDRC010000000
VERSION     DAWDRC010000545.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13964_length_2740_cov_2.218250"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          735 a          664 c          646 g          695 t
ORIGIN      
        1 gtggcgtgaa tgcgccaagc ggcaggatga agagcattat gacgccaaaa tgcagcctgt
       61 ccatgccaaa gtgttcgacc accggtttta atagtggtac caggatcagc aaaatggcgt
      121 ttccttccag aaacatcccc agcgtcagta gcaatatgtt gataatcaac aggaacatgt
      181 atttgttttc ggtcagcgaa gtcacaaaat tcgccactgc gacaccagca tgttccattg
      241 agaaaatcca tcccaatgcg gcgctggcca tgattaccag catcaccgat gcggttgcgc
      301 gtgccgtttc gcccaatgct ttgacgacat gagaaatccg catttcacga tagataaaaa
      361 agccaatcgc cagcacgatt agcaccgcta ctgcacctgc ttctgttggg gtgaaaatgt
      421 tagcgcgaat accacccacc acagcaaaaa tgaggaacag cgcaggccag gcctgtccca
      481 gcgagaacac cacctctttg cccgtaggcc agctttcacg cgagggttta gcgttagttt
      541 tgcgggcgac aaggtagacg gtaaacatca gtacgactgc gcatagcaaa ccaggtacca
      601 gtccggcgat aaacattttt ccgatagaga catcggcaac cagaccgtaa ataatcaggg
      661 ctattccagg cggtatgatc ggggtgacta acgagccacc agcggtaatg gcggcggcaa
      721 acgcgcgatt gtagccttta cgttccatct ccggcaccat catgcgcgac agcatcgccg
      781 cgtcagcaag gttagaggcg ctgacgccgc ccatcatggt gctgacgaga atgtttgcca
      841 cgcccagtcc gccgcgcatt ttccccacca gaacgttggc aatgtgcagg atgcgttcgg
      901 caatgccggt gtagctcatt agtgtgccga gcaagataaa aaacggaatt gccagcaatg
      961 atgggctttg tgttggcgcg attagtcttt gcacggcaat ttcaatcggt acgctggaga
     1021 agaataaaaa gtaagcgatg atgccaataa acatcgccag atagacccga atgttgagaa
     1081 acaaacagac gatcattagc ggaataatca ataaccaact cattgcattt tgtctccgct
     1141 atcggattgt tttgtatcgt ttattaacca gtagagggta taaatagccg ttgccagtgc
     1201 gccgacaggg accgccagat cgatccagaa ccaggaaacc ttcagaattt gtgtcacttt
     1261 aaactgcgcc atttcggcta gtcggtagcc cagccaggcc atataaagca gcagtgcgat
     1321 ggagagaagg gcgaccagta ctgcaataac acgcctgagt ttgcctggaa gcatctccat
     1381 caggaaggga atggtcaggt gcgccctgtc acgttctgcc accactccac ctaacatcac
     1441 cacccaaatc atcagcattc ccgacatttc ttctgtccat tgaagcggtt ggccgatgaa
     1501 ataacgcata aataccgctc caattgtcac cacaaccaga acgaatagcg caaaggcagc
     1561 actaaattct gccactttgc caaaaagacg catgaaacct cctgttagtg cgtgtcctgc
     1621 ctaagagaga tgaggcagga cgctgagcat taatgcttat tgcagttgct gttgaatggt
     1681 ttcgtacaga cctggtgtcc attccgggaa ttgagagtaa acttcgcgtg ctttcttctg
     1741 gaatggtcca gtatcgggat agatcacttc gacgccggct gcctgcatgg ttttcaacat
     1801 ttcggcatca cgctcaagag tgagtttctg gctatagaca cccgcttcat agccggtctg
     1861 gtggatcatc tccagttgtt ctggtgaaag ggtgctaaag aaggcttcac cacctatcca
     1921 cactgaggta tttgtcaggt agttaaccat tgtcagatat cttgcctgct catacagttt
     1981 ttgcccttgc agcactgaaa ttgggttttc tacgccgtca ataacgccct gggttaatgc
     2041 gggataaact tcgcctaatg gcatgggagt tggtgttgcc cccatcgcct gaatggcttt
     2101 tatttgcatg acgttattag gaacgcgaat ttttaaacct gcgagatcgt caacggtgcg
     2161 aatgggtttt ttcgaaataa tctgtcgagt gccatagaga taattattca ttaccacatg
     2221 aattcccttt ttcttcagac tttcattttt ttgtttaaac cagtcgcttt catatatttt
     2281 gaacagtttt tgcggatcgt cagtcagata cggtccaaac aaaatcccca aatcgggttc
     2341 ataatcggca agaaaagcca catcactcaa ggttatgaca ttcatgccca tcatggcttg
     2401 ctcggtgaca tcctgttttg atcccaattg ggagctggga tagagcgcaa gtgttatttc
     2461 gccattactt tttttattta ataggtctgc ccagtaacgc atgaccacat ccaggggttc
     2521 tccagggtta ttttcatagg caacttttat tgaaataggt tttgcctgga ttattgacgg
     2581 taaaaaaagg gtactgacag taattagcat cgctattatt ttgctcatct ttacttactc
     2641 catgctaagg aaataaaact ccaacattgt tgtcgggagt gaaaccttat gaaaaagcag
     2701 tcattactga aaagtagtca gcgaatagca gaatggctgc
//
LOCUS       DAWDRC010000546         2739 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13976_length_2739_cov_2.128539, whole genome shotgun sequence.
ACCESSION   DAWDRC010000546 DAWDRC010000000
VERSION     DAWDRC010000546.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13976_length_2739_cov_2.128539"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    445..454
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          536 a          702 c          814 g          677 t
ORIGIN      
        1 ggctgctcgc cggggtggtt ctcgccggtg cggtgcagga tttcatggtg ctgtttgttt
       61 ctacgcgccg tgacggtcgc tcgctgggtg agctggtcaa agaagagatg gggccaaccg
      121 ccggggtgat agcgctggtg gcctgcttta tgatcatggt cattatcctt gccgtgctgg
      181 cgatgatcgt ggtgaaagcc ctgactcata gcccgtgggg aacgtatacc gttgcgttca
      241 ccattccgct ggcgctgttt atggggatct acctgcgcta tttgcgtccg gggcgtattg
      301 gcgaagtgtc ggtgattggc ctggtattcc tgattttcgc cattatctct ggcggctggg
      361 tggcagaaag cccgacctgg gcaccgtact ttgactttac cggcgtgcag ctgacctgga
      421 tgctggtggg ttacggcttc gtggnnnnnn nnnnctctca accttcctga aaatcgggac
      481 tatcgtcggt ctggcggtag gcattctgat tatgcgcccg acgctgacca tgcctgctct
      541 gaccaaattt gttgatggca ctggcccggt atggaccggt aacctgttcc cgttcctgtt
      601 tatcaccatc gcctgtggcg cggtgtctgg cttccatgcg ctgatctctt ccggcaccac
      661 accgaagatg ctggcgaacg aagggcaggc gtgctttatc ggctacggtg ggatgttgat
      721 ggaatccttc gtggcgatta tggcgctggt ttctgcctgt atcatcgatc caggtgtgta
      781 cttcgctatg aacagcccga tggcagtgct ggctccggca gggacggcgg atgtagtcgc
      841 ttctgcggcg caggtggtga gtagctgggg ctttgcgatt accccggata cgctaaatca
      901 gattgccagc gaagtggggg aacagtcgat catttcccgt gcgggcggtg cgccgaccct
      961 ggcggtgggg atggcctaca ttctgcacgg tgctctgggc ggcatgatgg atgtggcgtt
     1021 ctggtatcac ttcgccattc tgtttgaagc actgtttatt ctgacggcgg tggatgcagg
     1081 tacgcgtgct gcgcgcttta tgttgcagga tctgctgggc gtggtgtctc cgggcctgaa
     1141 acggaccgat tcactgcctg ctaacctgct ggcaacggcg ctgtgcgtac tggcgtgggg
     1201 ctacttcctc catcagggtg tggtcgatcc gctgggcggt attaacacct tgtggccgct
     1261 gtttggtatt gccaaccaga tgctggcagg gatggcgctg atgctctgtg ctgtggtgct
     1321 gttcaaaatg aaacgtcaac gttacgcctg ggtggcgctg gtaccaacgg cctggctgct
     1381 gatttgtacc ctgaccgcag gctggcagaa agcgtttagc ccggatgcga aagtcggctt
     1441 cctggcgatt gctaacaagt tccaggcaat gatcgacagc ggcaatattc cgtcgcagta
     1501 tactgagtca caactggcgc aactggtgtt caacaaccgt ctggatgccg gattaaccat
     1561 cttctttatg gtggttgtgg tggttctggc gctgttctcg attaagacgg cactggtggc
     1621 attgaaagag ccgaagccaa cggcgaaaga aacgccatat gagccgatgc cggaaaatgt
     1681 cgaggagatc gtggcgcagg cgaaaggcgc gcactaaagt cagagtgagg gggaatgtgc
     1741 tgagcgttcc ccttactctc atcccgagcg tgttgatagc aattaagccc tcttaattca
     1801 ggagggcatt tgtattaccg gaggtttaga tgtttgattc actggcaaaa gccggaaaat
     1861 atttaggtca ggcggcgaaa ctgatgattg gtatgcctga ttacgacaac tatgtcgaac
     1921 atatgcgggt taaccatccc gatcaaacac cgatgaccta cgaggagttt ttccgggagc
     1981 ggcaggacgc gcgctacggt ggaaaaggcg gcgcgcgctg ctgctaaatt tgtacgtcag
     2041 gctttaaacg attccacttt ttcgaacgct gcacgcaacg tatctggtgt cagcgtgact
     2101 ggcaggtaat gaatggattc caccggacgt agggtgtggg caatcatttt gtcgatctcc
     2161 acctgattat tgatatccac ttccagctcc gccagcgtag tcggcagatg aaaacgctga
     2221 tacgcctctg ttaattgcgc cagcacatca tcctgaccca gcaaggcgct ttgcaccaga
     2281 attccgtaag cgactttggt gccgtggaga aacttctcgg tttgtggcaa aacggtcaga
     2341 ccgttatgca cggcatgggc cgccgccaca cgcgtaaaac gatcgcccag accaccgacc
     2401 attccaccac cagcaataat ggcatccacc acatcgcaaa atgattgcgt taattgctga
     2461 ttttgctgac cggcaagcgc ctgttcgcta ctgtttaaca agacgtcgcg aatggcctgc
     2521 gcattattga ttcccagccg cacggttagc ggcaacgttt ctggttgcgg agccagcacc
     2581 accgcttcat accatttcgc cagcgtgtca ccgatccccg ccagtagata ttcttgtggc
     2641 gcattgagga taatctccgg ttccaccagc accataaaat tggcgtcgtc gaaaatctca
     2701 taatgcagcg cttgtccggc atcgttatac cagacggag
//
LOCUS       DAWDRC010000547         2738 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13978_length_2738_cov_2.666045, whole genome shotgun sequence.
ACCESSION   DAWDRC010000547 DAWDRC010000000
VERSION     DAWDRC010000547.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13978_length_2738_cov_2.666045"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          740 a          693 c          588 g          717 t
ORIGIN      
        1 tttcaggggg tattttaaat aaaaacatta agttacggcg aagaagaacg gaaacgcctt
       61 aaaccggaaa attttcataa atagcgaaaa cccgcgaggt cgccgccccg taacctgttg
      121 gatcgacgga aaggacccaa aaacgagaat aattatcact tacagcaaga atcgaatctg
      181 atctattatg gtgcttgcta ttacgtgccg gcacaagtgc gtcgtttacc gtcatttcac
      241 acagaggcat catcaaatga aaatcagaaa tattctcgct atctcccttg cgacatcatc
      301 cttcagttgc ctggcattta aatcctcgcc caatgtgcta ccaggaccaa cgaatcaact
      361 aactgcggta gaaagtaaaa ttatcggaca tttttatgcc ccacacagta cattacccgg
      421 aacaaccatc acagggacat gtgacgcctc ccccgtcccg ggatgcacct gtccgttttg
      481 tactatgctg cgtagccaaa accgataaca tccgcattta cctggtattc catgatgagt
      541 tcacgcagcg actgatagag gaaggaaaga tggttagtaa aagcaaggcg cattgccgcc
      601 gcatgctgca ggcattgcaa cagacgagag caggtatttt tgaccagttg gaaaactgcc
      661 agcatacttt gcccgagtat atcgccatct catcggaaac cagtgcaact cttattcatc
      721 gggttccacc agagaaaaag aagaaatgaa cagtgaggcg ttgtgtggca tacaacgcct
      781 tcttccatca ttcctcgtca gccatgacaa aaatataacc gctggctctt tcatttttct
      841 cctgcttcca gcccctctct acctggaagc atcaaggacg tgacggcgta aagataaatt
      901 gtctcttcac tccctgacag gggcgattct tttcaaatcg ccatttcgcc atggccttca
      961 ccacttcatc acgaaacaaa ttatgaggct ctgagcggag aaaaacgatc cgtgtcacag
     1021 tcccatcagc accaatatcg aacttaacct caaccagccc cttgatataa tttgctgcag
     1081 catattccgg atatcgtgga tacaccgtca ctaattgccg gggctcatca gctttttgct
     1141 gcgagcatcc cactgccagg acagataaca gaaaaagtag taaaaggcgt cttttcattt
     1201 ttattcctac gggtcttatt ctgacaatat atcctgtgtt ccagactgcc acatcaccac
     1261 atcctgtgcc attatctgac tcacattaca tacatcgcat cgggatacag tagtagcact
     1321 ttctgtaata cagcttcctg tttcttccac catcgcaccg ggataaaccc gcgaatcatt
     1381 aacgcggtaa aaacccggtg tgcatcgttt ttaattattc ccgcacactc acgcagaagg
     1441 aattccccgt cgggctacgg tcatggttaa tgcgggaata cggcgacgat acagcgcagc
     1501 taaaagggta atggacggat agaccggttt atttcattcc acaggattct gagtgtcccc
     1561 aacttcctcc aatagtctga gcccacctgt gtagttttaa ttttcatcaa tccatttaac
     1621 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
     1681 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
     1741 atttactgtt ggaaacaaac ctacagcagt aacaccaaag gaaaccatca cccatcattt
     1801 cttcgtttcg ggaattgggc agaagaaaac tgtcgatgca gcaaaaattt gtggtggtgc
     1861 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
     1921 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
     1981 gttgcccatc gatatgggca gctctatctg cactgctcat taatatactt ctgggttcct
     2041 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
     2101 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
     2161 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
     2221 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
     2281 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
     2341 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
     2401 gaccatgaca ggcagacgat gatgcagata atcagagcgg agataatcgc ggtgactctg
     2461 ctcatacctc aatctctctg accgttccgc ctgcttcttt gaattttgca atcaggctgt
     2521 cagcattatg ctcgaactgg ccataaccag cgcccggcag tgaagcccag atattgctgc
     2581 aacggtcgat tgcctgacga atatcaccgc ggtcaatcat cggtaaagcg ccacgctctt
     2641 taatctgttg cagtgccaca gcgtcctgac ttttgggaga gaagtctttc aggccaagct
     2701 gcttacggta agcatcccac caacggaaag aagctggt
//
LOCUS       DAWDRC010000548         2736 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_13989_length_2736_cov_2.471839, whole genome shotgun sequence.
ACCESSION   DAWDRC010000548 DAWDRC010000000
VERSION     DAWDRC010000548.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13989_length_2736_cov_2.471839"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    318..327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          688 a          707 c          662 g          669 t
ORIGIN      
        1 tcttcaattg ttgaggttca cacgacaaag tccggcaaac ctaccccttc gaatagccta
       61 tgccagcaca caaaaagttt cgggaaaaaa aactttcttg taacgtgggg tcgcaggatt
      121 atagagaagt cggggtcaaa gatcgacccc tttttgtgat ttgtggcagg ttttaccccg
      181 ccaaatgctc gcgcttcaaa tactcttcgc tttgcatctc ttgcagacgt gacaggcaac
      241 gctggaactc aaacttcagc tgctcaccct gataaatttc atacagcggc acttctgcac
      301 tcaccactaa tttgacannn nnnnnnnctc gtaaaactca tccaccagcg caataaagcg
      361 tcgcgcttcg ctctccatca accgcgtcat gaccggcaca tcaaacaaca tcactgtgtg
      421 aaacagacgt gagagcgcaa tatagtcatg ctgactgcgg gcatcgacgc acaaggtagt
      481 aaaagaaact gccagcgtct ggttctcgac gcccattgtc gccaacggcc ggtggttaat
      541 ttctaacgtc ggtgaatttt ctcgtttcgc ccccgccagc gccaaccata gtttatccat
      601 ttgcgtccgg gtttcatcgt tgagtggcga aagccacaga tgcgcctgag tgagtgtacg
      661 cagtcgataa tcaacaccag cgtccacgtt cattacatca caatgctgtt taatggcatc
      721 gattgcaggc aaaaaacgcg cacgttgcag gccatttcga taaagttcgt ccggcggaat
      781 atttgacgtc gctaccaggg taataccacg ggcgaacagg gctttcatca gaccgccaag
      841 tagcatggca tcggtaatat cagaaacaaa aaattcgtca aaacagagca catcagtttc
      901 tgctttaaag cgatcggcaa taatttccag cggatcgctt tggccttgta attcggtcag
      961 ctcatcgtgc acccgcagca taaaacgatg aaagtgcagg cgctgtttcc gctctcccgg
     1021 cagactttga tagaaaaggt ccatcagcca ggttttcccg cgtcctacac cgccccacat
     1081 atataagcca cgcactggca tatgctttgt gtcttcgcgt ttaccccaca gcttaccgac
     1141 ccgcgccatt agcccactcg tcctgggggc tggtggcgtg ctattgatga gttcctgata
     1201 aataatttcc aggcggctga cggcctcttt ttgaacgtcg tcgtgttgat ggctgccttc
     1261 attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt tgcatgatct tattgttatt
     1321 ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa aaaaggccgt tctacactac
     1381 gcgatatgct gtcgggattc cacttctgtg gaattaacgg ttatagtggc ataatcagcc
     1441 gcaggcatgg agcctgaagc caacacccta cggaaacaaa agacaacggg agatgttcat
     1501 gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc attattggtg ctgtggccat
     1561 gcgttttggt aatcgtaaac tacgccagca acaggcgttg cagtacgaac tggaaaagaa
     1621 taaagctgaa ctggacgagt atcgcgaaga gctggttagc cactttgccc gcagcgcgga
     1681 attactggat accatggcgc acgactatcg ccagctgtat cagcacatgg cgaaaagctc
     1741 cagtagcctg ctgccggaac tgtctgcgga agcaaacccg ttccgtaatc gtctggcaga
     1801 gtctgaagcc agcaacgatc aggcaccggt gcagatgcct cgcgactatt ctgaaggcgc
     1861 atccggcctg ctgcgtactg gcgcgaagcg cgactaattt atttttcggg cgcagccatt
     1921 gcgccctcct cttctctccc tccccgacta tcatttaatc tggtgtctca ttgttagccg
     1981 tctgaaaatt caataacatc aaactgtttt gaatctcttt tcttatcatt caggtacgag
     2041 agcaggaata atgaaaaaac aaacccagct gttgagtgca ttagcgttaa gtgtcgggtt
     2101 aactctctcg gcgtcatttc aggccgtcgc gtcgattcca ggccaggttg ccgatcaggc
     2161 ccccctcccc agtctggctc caatgctgga aaaagtgctt ccggcagtgg tgagcgtacg
     2221 ggtggaagga acggccagtc agggacagaa aatcccggaa gaattcaaaa agttttttgg
     2281 tgatgattta ccggatcaac ctgcacaacc cttcgaaggt ttaggctccg gtgtcatcat
     2341 caacgccaat aaaggctatg tgctgaccaa caaccatgtg attaatcagg cacagaaaat
     2401 cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa ctgattggta gcgatgacca
     2461 gagcgatatc gccctgttgc aaattcaaaa cccgagcaaa ttaacgcaaa tcgctattgc
     2521 cgactccgat aaattgcgcg tcggtgattt tgccgtggcg gtcggtaacc catttggcct
     2581 tgggcaaacc gccacctctg gcattatatc cgcattaggc cgcagcgggt tgaatcttga
     2641 aggtctggaa aactttatcc agacagatgc ttccattaac cgcggtaact ccggcggtgc
     2701 actgctgaac atgaatggtg aggttatcgg tatcaa
//
LOCUS       DAWDRC010000549         2720 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14074_length_2720_cov_2.515947, whole genome shotgun sequence.
ACCESSION   DAWDRC010000549 DAWDRC010000000
VERSION     DAWDRC010000549.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14074_length_2720_cov_2.515947"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          627 a          699 c          648 g          746 t
ORIGIN      
        1 cctatttcag tgcaggaatc gagttattct ggttattcaa cgcagactca agctgactaa
       61 agcgataatt gaaatcttcg ctattcacga tcagatattt attattggtt ttgatatcgt
      121 caaccacccg cccacgatcg ctggctgccg cctgaaaaga gtaaaagttc accgaccagc
      181 cagacgcggg cgttccttta ctggttaaca tcgccgagaa tttcaccggt ttatctttcg
      241 tccaggtctg ttcgagcagc tcgtaatctg ccaactgacc gacccaggta tagagatcgt
      301 cactggaaga gacatcgcct tccgctgacc aggtggcctg attaccctta gcatcaagat
      361 tttttaaggt gatgacatca agcttgagaa tgccgtgata ctgctttttg aattgatctt
      421 ttaagatatt ttcagttggc ggttcaccat ctgctcggac attactagtc atcaacataa
      481 acagcgccgt accaacaatc cattgctttt tcacccgaaa accctcgcgc ggaataattt
      541 attccggtga ttatcattag ggtaataaaa attggcaatg gacgtgagtc tgaagtgaat
      601 aagccccggc acgataccgg ggcgaagtga ttagtactga ttgaagatct gctggatctg
      661 ctgcggatct ttagtttgcg tcagagccag ttgcagcaga acgcgcgctt tttgcgggtt
      721 caacgtgcca gaggcgacga agccgtattt cgcatcatcc acttcggcat cctgagtggt
      781 agcgcccgtc ggtacgcggg aagaacgcac tactgcagtg ccgtttttcg cagcggttgc
      841 aagggtgtca aatacggttt tatacaggtt gccgttaccc acgccagcgc taacgatgcc
      901 atcatagccc gcatctacca gtgctttagc cggaagatcg gatgcgttag cgtagttata
      961 aacaatgccg actttcggca gttcattcag cttagagaca tcgaacggcg tgtcgctggt
     1021 gtgcttacgt gccggggtac gttggtaatc aatcttaccg ttgtgaatgt aacccagagg
     1081 accgtagtta acagacttga aggtcgctac gtcggtggtg ttggttttgg tgacatcacg
     1141 gccatcaagc acggtgtcgt tcatcactac cagtacgcca cgattagcgg aggctttatc
     1201 agttgcggtc actaccgcgt tatacaggtt gaatggaccg tctgcgctca tagacgtgga
     1261 cgggcgcatt gcaccgacca tcaccaccgg tttgtcgcat ttcaccgtca ggtcgaggaa
     1321 gtaagcggtt tcttccatcg tgtcggtacc gtgggtaatg acgaagccgt cagttttatc
     1381 gcagtcggtg ttaatttttt tcgccagtgt cagccagaca tcatcgttca tgtcctggga
     1441 gccaatattc actacctgct cgcctttaac gttcgcaatg tccttcagtt gcggcaccgc
     1501 attaaccaga ttttctacgc caactttacc cgctgtgtag ttagatttgg ttgcggagtc
     1561 accaccaccg gcaatggtcc cgccggttgc taaaatggtg atattgggta atgccaatgc
     1621 tgcaccacta aaacccataa ccagtgcggc aagtgccgtc tttttgaaaa actccatttc
     1681 attcctccag ttacgtgaac gctacgcatt atcccttagc tttgtatggg aaacttgacg
     1741 ttaaacaatt tacaacatga atatattttg gagatctaca aagttagagg caggtaacaa
     1801 aacgaagaat taaatggcat aaaaaagtat tatgccgtct tagaatagag gattatttta
     1861 aattcccgac cagggctttg cggctatctt ccagagtcac aacgcggcta caaacatctt
     1921 tgccaaactg ctggaaatct ttttcctgct ttttccactc ggtttggatt gaggattgca
     1981 gaccgcccag gcttcccagc acgttctgta atgggttacc gccgcttttc agcaccgctt
     2041 tcgcgcccat ttcattaatg ctgtcctgta aaattccgcc cattgcctga ttcactaatt
     2101 gctggccttc ggcacgaacc tgatcaatgg ctttatagtg aaacgtcagg ccatcgctgc
     2161 gcgtttcgat aatgcggttc atctgctctt tcagctgcgc atcaagtttg gtcagacggc
     2221 tgcgcatttt gctgctttcg cccatctcct gaacgataat tttatccagc gcaatacggg
     2281 ctttctcgac gcggcttttc gcgccttcat caatccacgg cagggtgcta cgtagttcag
     2341 cctgataatc cttcgcctgc tcgcgctggg cggcattcag ggaatattgc ttaccgttat
     2401 acatcacgtt gccgtctggc gtgatcacca gattgccgtt ttcgcccttc acctgcacgg
     2461 tttgcgggct gacaatcaca tcgtcacgcg gcgtgacgct gcactggtag tcggcgtgag
     2521 cggtcattgc cgtcactgaa agtgctgccg ccagcagcat tttgcgcatc atagtcttcc
     2581 ctcaagaaca aatcaggcca gcatttgctg gccccagatt gataacaaag tgcgcgtttt
     2641 ccatgccgga tgcggcgtaa acgccttatc cggcctactc gatcgtgcat ctttgtaggc
     2701 ctgataagcg tagcacatca
//
LOCUS       DAWDRC010000550         2719 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14078_length_2719_cov_2.429429, whole genome shotgun sequence.
ACCESSION   DAWDRC010000550 DAWDRC010000000
VERSION     DAWDRC010000550.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14078_length_2719_cov_2.429429"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1337..1346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          769 a          682 c          632 g          626 t
ORIGIN      
        1 ctctccgcgt gcggaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc
       61 aatcgggatt atcgcgggtg cggttaaagg cctgaatgaa gagcaaggta acgtggtttc
      121 tcgcttcggt ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcatcaat
      181 cgcagcactg gtgctgtaag accatacata aaaaagccgg ggataattcc ccggcttttt
      241 tacgcctgtt aatcagctaa tggctgcgga cgaccaatta aatatccttg cagatattgc
      301 accccgagct tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca
      361 ctcaatgact tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgtg
      421 ttcgtgacga tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc
      481 tttaaccgtt cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca
      541 aacttatgca gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc
      601 tcggtgatct cgagaatgac cgcctgcggg gagatgtaat aacgcttaaa cagacgaata
      661 atccgcccgg caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaatcgt
      721 ggaccttttt tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc
      781 aaatcaaaac gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc
      841 ataatgccgc cgtcatactt cagtcgcgcg aggatctcat cataaccttc accctctttg
      901 ttgcgaattg gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta
      961 ttcagcaata gcacctgttt agtggtttgc ccggaaacca tctcctctct gctatccagc
     1021 gccagcacat gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag
     1081 ggttgcaggg tttcctgatt tccatcaaaa cgtccccagg cagcaccata gcccatatcc
     1141 agcccggtat tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt
     1201 cgcccttccg tttccggccc gctcagcact aacagcagtt cactacccgg caattgatac
     1261 aacttttcgt tttcctgcat caacggcagc agcgtacggt aaattgagcg gatacagtga
     1321 acgcgcatca ttaagcnnnn nnnnnngtaa tgacgactca taaactcaag attatcaatg
     1381 cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgctccaa cgcacgaaag
     1441 ttgggtagaa gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg gttaagccat
     1501 tcactacgat gataaatacg caccatatag agcaggcaaa cactaaagga aatcagtact
     1561 gcgagaataa atgccagcga atattcggtt agtacccctt gcaaaaagtt ctggttgtaa
     1621 ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata gcgaagctta
     1681 ccgacaccga gggtaaagat gataaagaaa acaggtacca ggtaaccggc aataaagtca
     1741 ttttcatacg gtgtgcacat cagaagtagc agcacgctaa gagctgccag ccagcttaag
     1801 gtaaatgcgc gtttctcttt gctcaacgac ggagcgatat ccctacgcca caatatcagt
     1861 gcaaagtggg gacttacaat catgcgggtg agatagtaaa aaagcatgtt gtaaatcagc
     1921 actgcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa
     1981 aaggtagata tctttagcgg aaaatcaaag aaatttccca caagatacat gctgcatttg
     2041 atgccaatcg gcgccaccaa accaagccag aacagacgtt gccagacgta tcggctggtc
     2101 aatccataac gccaacgcgt ccccaattgc cagcgtaata ttgcacaggc acacaggact
     2161 gcaaacgcct ggctgccgat catgacggcg gtctgtaaaa aagtgagatg aaggttccac
     2221 agattggtgc aaaacatccc gcataaaatg ggaacgacac cacgccagcc aaagataaac
     2281 aagattgaca gcattacgca cagtggcatc cacgccagaa agatataatt ggaatggata
     2341 acagcaagtg gcgaaattaa acgggatagc tgaataagta ccacggtgag cgtaaacgct
     2401 agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag tacactactc
     2461 tcactgatcg gggttttcag acacacaaat aataagttta ttcgcatcgc gaagcaaaca
     2521 ggggccagcc tgttgctgtt ttttcagtca gtgatgcatt caccacacgg cactgccagg
     2581 atgtagactg tattacaaaa gcggcaaaag gcagagacaa aaaacccccg ctttgcagcg
     2641 agggtttgaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc
     2701 aagcgctctc ccagctgag
//
LOCUS       DAWDRC010000551         2715 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14103_length_2715_cov_3.171053, whole genome shotgun sequence.
ACCESSION   DAWDRC010000551 DAWDRC010000000
VERSION     DAWDRC010000551.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14103_length_2715_cov_3.171053"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          745 a          503 c          623 g          844 t
ORIGIN      
        1 aacatttata tgagataacc cacatgaaac aaaagaaagc ctggagtttt tttcagagcc
       61 tggggaaggc atttatgtat cccattgctc tgctaagtgt atgtggcatg atgctagggc
      121 tgggaagtgg tttagccagt gatgatatgg caaagttaat tccatttctg gctattccaa
      181 taattaaaac catacttgat ttcattgtta gtcttggttt gtttgccttt gttaatttac
      241 ctgtattgtt tgcgatagcg attcccttag gattattaaa agataaagag gataaagcct
      301 atggtgcttt ttctggctta attggtttta tggcgatgca tctgggaacg aacttttatc
      361 ttaaacagca cgacttattg gtcgttgctg accaaatgtc gacacatggg caaaccatca
      421 ttctggggat ccagtcctac aataccagcg tgttgggggg aattgttgct gggttattag
      481 tcgccagcat gtataaaaag atcgttaatt tacgcattcc agaatcatta ggtttttata
      541 gcggcccacg tctggtgcct atcattacac tgattgtgat gagtggattt ggtctgatca
      601 ttccttttat ctggccgccg tttttcaatc ttttcatgct cattggacac tggatttcaa
      661 cttccggtcc tgttggttat ttcttctatg cagttgccga acgtgtgacg attccttttg
      721 gcttaaacca tctggtgacg tcagttttcc gctttacgcc aatcggcggt tcggctgtga
      781 ttggcggcga agaatattac ggcaccctga acatgtttat ggcatacgtt aaagagaatg
      841 cggtcattcc gctggatttg gcggggaaaa tggagcaggg caaactgatg attcagtatg
      901 gtctggctgg tgccgcgctg gcgatgtatc gcactgctca tgctcaaaac agaaaggcta
      961 tcaaagcatt gcttatttcc ggggtgctta cggtgattat tggcggcgtc agcgaaccga
     1021 ttgagtttct gttcttattt gtcagtccac tgctgtttgt cttccatgcc tttatgaatg
     1081 gattcgctaa catggttctg ccatatatgg gcgtgaagat gggatttact ggcgatctga
     1141 ttcaatttat tagctttggc gtattgcgtg gcacaagaac aggttggccg atcgcggtgt
     1201 gtgtcgaagt ggcctatttc ttcatttatt actttgtgtt ccgttggacc attcttaaat
     1261 ttaacctgat gaccgtaggc cgtgaagagt ccaatcctgt cacgctgaac gttcacgaag
     1321 atacggctat agcggatatc ccaactcctg ataaatcaga gctgcaagcg gcggagcaga
     1381 tggttaaggc acttggtggt aaagagaata ttaagtcact ggataattgc gtaactcgtt
     1441 tacgtttaac aatcgcagat atgggattga ttgacgaagt tgcaataaaa agagctggcg
     1501 ggattgcggt tgttaaactt gatcaaaata ccctacaagt cattatcggc actaaagtca
     1561 tcgccctgcg tcgggatatg gataactata tggggatata ctgatggatg tttttaatac
     1621 tcctgtttcc cgcaaaggaa catattgtac ccaatgggac ttttgcgagg acagatttgg
     1681 tgtaaaagat gtactaccat tctctatttc agatatggat cttccgatac cagacgctat
     1741 caccagagca ttaaaaaaac gcctggaaca cccgatttta gggtacagtc gttggcaaca
     1801 tggtgaatat ctgaatgcga tagtaaactg gtattatcaa caatatcaaa cagatattaa
     1861 acctgaatgg ataacctata gccccagtgt gatgtactct attgcaaaag caatagagtt
     1921 attgacatcg catggtgata atattctcgt ttttacacct gtctataatg cattttttga
     1981 tgtaattaaa catagcgagc gaaatattct gacagcctct ttgattataa aaaatgaggg
     2041 gggctacgct atcaactggc aagattttga tcttaagatt aaaagcgcaa agatggtgtt
     2101 attatgtaat cctcataacc cgacaggaac agtctggtcg gaagaagaac tgcataaaat
     2161 cgctgcaagc tgtacaaggc ataatgtctg gttatgttca gatgaaatcc atagtgattt
     2221 tgtttttaat cgtagcttta catccgcgct taaaataaaa aaagaaaaag tagtagtatt
     2281 taactcgata tcgaaaacat ttaatgttcc tgcactaaca ggatcgtata tgatttcaac
     2341 tgacgataat tttaatcata agttcagaac gatatcgagg tatcgcgatt ttgtaaactc
     2401 cccatcagta ctgaatataa ttgcaactat tattgcctat aacgaatgtg agggatggtt
     2461 aaagtcatta aaggcacata ttgcctctaa cattcaattc acccagcagt atctgaatga
     2521 aaatattcca gaattgatcg taaggccagc tgatggttgc tatttttcct ggatagattg
     2581 ttctgctatt ggttatcctt ttgatgaatt ctacagtcga ttaattcatg aaggcaaagt
     2641 gggcattatg gcggggcatg tctatggaac ggagggggag ggctaccttc gtctgaacct
     2701 ggcctgcggt cgaga
//
LOCUS       DAWDRC010000552         2711 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14126_length_2711_cov_2.204066, whole genome shotgun sequence.
ACCESSION   DAWDRC010000552 DAWDRC010000000
VERSION     DAWDRC010000552.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14126_length_2711_cov_2.204066"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          668 a          681 c          688 g          674 t
ORIGIN      
        1 ttacgttaaa aatgtttaat gttatttaat ggttgttaat ttgaatactt cgataatgtt
       61 atatttcctg ataatcattt gcaggcaaaa tgttttcacc tttaaatgag tatttattct
      121 cataaatcga aaaaggattc attatggtta cactttacgg catcaaaaat tgtgacacca
      181 ttaagaaggc tcgccgttgg ctggaagcta ataacatcga ctatcgtttt catgattacc
      241 gcgtcgatgg attggacagc gaattattga acggttttat caacgaatta ggctgggaag
      301 cgttactcaa cacccgtggt acaacctggc gtaaactgga cgaaaccacc cgcaataaaa
      361 tcaccgatgc ggcctctgcg gcgacattaa tgactgaaat gcctgcaatt atcaaacgtc
      421 cattgctctg cgcgcctggt aagcctatgc tgctgggttt cagtgaatcc agttatcagc
      481 aatttttcca tgaggtgtag tctatgtcgt gcccggttat tgagctgaca caacagctta
      541 ttcgccgccc ttccctgagt cctgatgacg caggatgcca ggctttgttg attgaacgtt
      601 tgcaggcgat cggttttacc gttgaacgca tggactttgc cgacacgcag aatttttggg
      661 catggcgtgg gcagggtgaa acgttagcct ttgccgggca taccgacgtg gtgccgcctg
      721 gcgacgccga ccgttggatc aatccgccgt ttgaaccaac cattcgtgac ggcatgttat
      781 ttgggcgcgg tgcggcagat atgaaaggct cgctggcggc gatggtggta gcggcagaac
      841 gctttgtcgc acaacatccc aaccatacag ggcgactggc atttctgatc acctctgatg
      901 aagaagctag tgcccacaac ggtacggtaa aagtcgtcga agcgttaatg gcacgtaatg
      961 agcgtctcga ttactgtctg gttggcgaac cgtcgagtat cgaagtggta ggtgatgtgg
     1021 tgaaaaatgg tcgtcgcgga tcgttaacct gcaaccttac cattcatggc gttcaggggc
     1081 atgttgccta cccacatctg gctgacaatc cggtacatcg cgcagcacct ttcattaatg
     1141 aattagtggc tattgagtgg gatcagggta atgacttctt cccggcgacc agtatgcaga
     1201 ttgccaatat tcaggcggga acgggcagta acaacgttat tccaggtgaa ctgtttgtgc
     1261 agtttaactt ccgctttagc accgaactga cggatgagat gatcaaagcg caggtgcttg
     1321 ccctgcttga aaaacatcaa ctgcgctata cggtggattg gtggctttcc gggcagccat
     1381 ttttgaccgc gcgcggtaaa ctggtggatg cggtcgttaa cgcggttgag cactataatg
     1441 aaattaaacc gcagctactg accacaggcg gaacgtccga cgggcgcttt attgcccgca
     1501 tgggggcgca ggtggtggaa ctcgggccgg tcaatgccac gattcataaa attaatgaat
     1561 gtgtgaacgc tgccgacctg cagctacttg cccgtatgta tcaacgtatc atggaacagc
     1621 tcgtcgcctg atgagtggtt ctgcaagagg aaataagcat ggactggctg gctaaatatt
     1681 ggtggattct ggtgattgtc tttttggtag gcgtcctgct gaacgtgatt aaagacctca
     1741 agcgcgtcga ccataagaaa tttctcgcca acaagccgga gcttcccccg catcgtgatt
     1801 tcaacgataa gtgggacgat gacgacgact ggccgaaaaa ggatcaaccg aagaagtaag
     1861 ctaagtggcc ggataaggcg ttaacgccgc atccggccat gatgcctgat acagtcttat
     1921 cagacctacc cggctattat tacatcattt caatcacatc atcatcgcca ggcttaccgc
     1981 cgcttagcgc ttcgtcgaaa taatgtttcg gaatggtata acgcaagtga tccagggcta
     2041 attgcatact gcgattgtcg atcgcatggc ctaaatcctc cacgatatcc agcgtcacat
     2101 cgccaccggc actgattaac gcttcctgcg ccgcaaccgc atgcgccaga tcgataaccg
     2161 gatcttcacc gccatgaatc aagtgaattg tcgtggctgt agacgcggtt tccggcaggc
     2221 tcgcctagcg tccgttgaag gcaatgacgc gcgaagcaag gcccggttcg gctttaatgc
     2281 tctccagcgc cataatcgcg ccttgtgaga aaccgattag cgccgtggcg ttagccccta
     2341 ctccgctctg tttctgccag tagcgcaccg tttcaataaa cgtcggcatg atggcatcca
     2401 cacgcgcctg gcgattatct tccgtaatac cctgcaccga aaaccactga cgccccgccg
     2461 gattaccgct cggctccgcg ccaccgacac tcactaccag cgcatcagga aacagcggcg
     2521 caaaccagct acctatttcc cccattgcca ccgggttatc cccgacaccg tggaaaagca
     2581 gtaacaactg ttgtgctggt ttatccgggc tttgaacaac aaaatggtca tgtttcatgg
     2641 cgaactcctt aactgacgtc attaatttta cgccgctgac tcacaatgac catgggagaa
     2701 aattgaagga g
//
LOCUS       DAWDRC010000553         2710 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14135_length_2710_cov_2.098305, whole genome shotgun sequence.
ACCESSION   DAWDRC010000553 DAWDRC010000000
VERSION     DAWDRC010000553.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14135_length_2710_cov_2.098305"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    272..281
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    838..847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2442..2451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          704 a          700 c          681 g          595 t
ORIGIN      
        1 tacccagtat gacgatagca atatgggcca gaacaaagtt ggcctgagct tctcgctgcc
       61 gatttatcag ggcggaatgg ttaactcgca ggtgaaacag gcccagtaca actttgttgg
      121 tgccagcgag caactggaaa gcgcgcatcg tagcgtcgtg caaaccgtac gttcctcctt
      181 caacaacatt aatgcttcta tcagtagcat taacgcctac aaacaagccg tagtttccgc
      241 tcaaagctca ttagacgcgc tggaggcggg tnnnnnnnnn ncgggttact cggttggtac
      301 gcgtaccatt gttgatgtgt tggatgcgac caccacgctg tacaacgcta agcaagagct
      361 ggcaaatgcg cgttataact acctgattaa ccagttgaat attaagtcag ctctgggtac
      421 gttgaacgag caggatctgc tggcactgaa caatgcgctg agcaaaccgg tttccactaa
      481 tccggaaaac gttgccccgc aaacgccgga acagaatgct attgctgatg gttatgcgcc
      541 tgatagcccg gcacccgtcg ttcagcaaac atccgcacgc actaccacca gtaacggtca
      601 taaccctttc cgtaactgac gacgacgacg gggcttcggc cccgtctgaa cgtaaggcaa
      661 cgtaaagata cgggttatct gccgcattct tcccccttct cgcttcaatt tcgaccagcc
      721 atcctctatt ctgatgggta tttaccactg gtcccggaag acaaaaatga aacggacaaa
      781 atccatacgc cacgcatcgt tccgcaaaaa ctggagcgca cgccgtctga caccagtnnn
      841 nnnnnnnatg ctggctggct gtgaaaagag tgatgaaaca gtgtctctct atcaaaatgc
      901 tgacgactgt tcagctgcaa acccaggcaa aagcgccgaa tgtaccaccg cgtacaacaa
      961 tgcgctgaaa gaagccgaac gtactgcgcc gaaatacgcc acccgtgaag actgtgttgc
     1021 tgaatttggt gaaggtcagt gccagcaggc accagcccag gctggcatgg caccagaaaa
     1081 ccaggcgcag gcccagcaat ccagcgggag tttctggatg ccgctgatgg ccggttacat
     1141 gatggggcgt ctgatgggcg gcggcgcggg atttgcacag cagccgctgt tctcctcgaa
     1201 aaacccggcc agcccagctt acggtaaata taccgacgcg accggtaaaa actatggcgc
     1261 agcccagcca ggccgaacca tgaccgtacc gaagacggca atggcaccaa aaccggcgac
     1321 cactactact gttacccgtg gcggttttgg tgaatctgtt gccaaacaaa gcactatgca
     1381 gcgtagcgca accggcacct cttctcgttc aatgggtggc tgataccgat ggaaagagtc
     1441 agtattaccg agcgcccgga ctggcgcgaa aaagcccacg aatacggttt caattttcac
     1501 accatgtacg gcgagccgta ctggtgtgaa gatgcttact acaagttgac cctcgcccag
     1561 gttgaaaagc tggaagaagt caccgccgaa ctgcaccaga tgtgcctgaa agtggtggaa
     1621 aaagtgatcg ccagcgatga gctgatgacc aaattccgca ttccaaaaca cacatggagt
     1681 tttgtgcgcc agtcatggct gacgcaccag ccatcgcttt attcgcgtct tgatctggca
     1741 tgggatggta ctggcgaacc taaacttctg gaaaataacg ccgatacgcc aacgtcacta
     1801 tacgaggcgg cgttctttca gtggatctgg ctggaagatc agcttaacgc cggtaacttg
     1861 ccggagggca gcgaccagtt taacagtttg caagaaaagc tgatcgatcg cttcgttgag
     1921 ctgcgtgaac agtatggctt ccagttgctg catctcacct gctgtcgcga cacggtggaa
     1981 gatcgcggaa ccattcagta tttgcaggac tgcgcgacgg aagctgaaat cgctaccgag
     2041 ttcctctaca tcgacgatat cgggttaggt gaaaaaggtc agttcacgga tttacaggat
     2101 caggtgattt ccaacctgtt caagctgtat ccgtgggaat ttatgttgcg tgagatgttc
     2161 tccaccaagc tggaggatgc aggcgtacgc tggctggaac cggcgtggaa gagcattatc
     2221 tccaacaaag cgcttctacc gctactgtgg gagatgttcc cgaatcaccc gaacctgctg
     2281 cccgcttatt ttgcggaaga tgatcatccg caaatggaaa aatatgtggt taaaccgatc
     2341 ttctcccgtg aaggcgcaaa cgtgtcgatc attgagaacg gcaaaaccat tgaagcagcg
     2401 gaaggtccgt atggcgaaga agggatgatt gttcagcaat tnnnnnnnnn ncacccgtta
     2461 ccgaaattcg gcgacagcta tatgctgatt ggtagctggc tggtgaacga tcaacctgcc
     2521 ggaattggca ttcgtgaaga ccgtgcattg atcacccagg atatgtctcg gttttatcca
     2581 catatttttg ttgagtaata cagcttaata ttgccccgat aagcggggca atctcttatc
     2641 atccaccatt cataattccc atcaatatat aaacacatac atatccaacc tttatttaac
     2701 attaatactc
//
LOCUS       DAWDRC010000554         2700 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14179_length_2700_cov_2.341777, whole genome shotgun sequence.
ACCESSION   DAWDRC010000554 DAWDRC010000000
VERSION     DAWDRC010000554.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14179_length_2700_cov_2.341777"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2020..2029
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          680 a          674 c          672 g          664 t
ORIGIN      
        1 cactggcaat gaggtaggct ggctggcaaa ccagacgcgc cccagtagtt gcgggttaag
       61 caccaggccg ctgaacagat cggcgaaaaa ttgttgatcg gcgggcaagc ctggtgcttt
      121 catgaaagtt cccgattaaa gatgcattaa ctggcggaat tctttaactt tgctgcggct
      181 gaccggcact tcaaaatcca gatctttcag ccgcagaatg taggtgttat taaaccacgg
      241 ttcgatctcg cggattttgt tcagattgac acaaaatgag cgatggcagc ggaaaaaatg
      301 cgacggcggc agcttgctgc aaaactcggt aatgttcatc ggcattacgt aggattcacg
      361 ccgcgtatag acaaaggtca ttttctcgtg cgcttcggcg taatagatat cgttaattgg
      421 cgtgacgatg attcgctcat ctttcaccag attaatggtg tcattctcac gcgttaccgt
      481 cgcggcaggc gtcgtactgc tggtctgctg ttgttgccag gctgcttcca gtttttgcag
      541 catcccggta atgcgtgatt cctgatacgg tttgagaatg tagtcaaacg cttccagctc
      601 aaatgcttcc accgcatgtt ctttccacgc ggtgatgaac acaataaacg gtttatgagc
      661 aaactggctg atgttttgcg ccagcaacac accgtccagc gacggaatat tgatatccag
      721 aaaaatggcg tcgacgcggt tatgctgcaa aaacttcaac acgtccagac cgtcgtcaaa
      781 ggtgccgaca atctccatct ggctgtgctc tttaattagc cagctcagtt cctgttgtgc
      841 caggaattcg tcttcaacaa tgatgacttt cacaatatca ctccggctca aagcaataag
      901 gtagcctgtg aggcgactgg ggtgcgttgg ttaggaatgt aaaacgcaat ttccgttcct
      961 ggttccaggc ggcggatatg cagcccctcg ccatacaata acttcacgcg atgatggaca
     1021 ttcagcaggc caattttatt gcccggcatt tcgttcgctt cgacccgctc aatcaccttt
     1081 ggatcgatgc cgtgcccggt atctcgcacc gcaatgcgta cccgattacc gcactcagca
     1141 acgctgatgg tgacaacgcc tttacctttg caaggctgga taccgtggac aatggcgttt
     1201 tccaccaacg gctggatcag caggctggga atgcagcaat tcacctcttc atcaatatca
     1261 tagataaccg tcagcttgtc gccaaagcgg gcctgctcaa tggcaatata gtctttaatt
     1321 tgatacagct cttttttgat atcgatttgc tcatcgtctt ttaattcaat gttatagcgc
     1381 agatagcgcg acaaattaaa gatcagctgg cgagcagtat ccggattcag acggattgac
     1441 gatgaaatag cgttcaaagc gttaaacaga aaatggggat taattttgct ttgcagggcg
     1501 cgaagctctg ccttatttgc catttcacgc agctgctcag cgcgcgaaac ctccagttgc
     1561 gtggagatga tttgtgacag accgaccgcc atttcctgta atgacgaggt tatctgatgc
     1621 gcgtggcagt agtaaatttt cagcgttccg gtcacaacgc ctttctccca caatgggatc
     1681 accagcatgg aatgaatctc tggtgtgcgg tgggcttcat cattgttttt aatgatgatt
     1741 tttccgtaat tcatcgcctg tcgggtagtc gggctaatga agtcatcgcc attctgatag
     1801 ttgtgttcac ccacgccgac ataggccagc acatgatcag tattagtcat cgccaccgca
     1861 tcggcgtgaa tatcatcgcg aataatttca caaactttgc gtaatgactc gctattgaca
     1921 tggcgaaaca gcggtagcgt tttgttggcg atatccagcg ccagcttggc ctgccgcgcg
     1981 gcgctggcct ctttttcgcc ctcaacgctt tgcaccagcn nnnnnnnnna aagccgatac
     2041 agacgctacc gaggatcatt ggaatcccga ttttagagac gatatcgatc cccaatgcgg
     2101 tagtgggtgc ccagacaatc accagaatca tggtcaacgt ttcacacaac atgccgccga
     2161 gaataccgac gcgccagcgc tgtgctttgg ggattttcag gttgatccag cccgatatac
     2221 aacccgccag aatgctggtg ataaagcagg ggatcgccgt cacgccgccg atgtcaatta
     2281 aataacggtg aatacccgca atcacgccgg taatgatgcc cacccacggg ccaaacagaa
     2341 ttccgcctga catcaccgcg ataatacgta cgttcaccag cgagccttct acgggaacgc
     2401 cggaccaggt gctgaacagg gcgaacagcg agaaaatggc ggtaacggcg agcagctctt
     2461 tcggtgagtg tgccgacttg tgcaacagtt cgcgaaacag gcggatacgg atgagaaaaa
     2521 acaggcagat aagcattaac gctgcccgat cgaagaccgc cagcagcatg ttgaatattt
     2581 cgtgcacggg tgactctgaa taaggattaa acctctatga taggtaacct gaaggctgat
     2641 gaccagcagg ccgtttttga ggaaaaacca caattttaaa aggggattga aagataaatg
//
LOCUS       DAWDRC010000555         2692 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14230_length_2692_cov_2.228290, whole genome shotgun sequence.
ACCESSION   DAWDRC010000555 DAWDRC010000000
VERSION     DAWDRC010000555.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_2692_cov_2.228290"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1719..1728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2247..2256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          591 a          722 c          724 g          635 t
ORIGIN      
        1 tgcgaacgtc agattgctcg ccgtcaagat tgaaatagac gtgattagtc atattcaccg
       61 ggcaaggttt atcaactgtg gcgcgataag taatggagat acggttatcg tcggtcagac
      121 gatattgcac cgtcgcgccg agattacccg ggaaaccctg atcgccatca tctgaactca
      181 gggcaaacag cacctgacga tcgttctggt tcacaatctg ccagcgacgt ttgtcgaacc
      241 cttccgggcc gccatgcaat tggttgacgc cctgacttgg tgaaagcgtc acggtttcac
      301 cgtcaaaggt ataacggcta ttggcgatgc ggttggcata acgaccaata gaggccccca
      361 gaaatgcggc ctgatcctga tagcattccg ggctggcgca gccaagcagc gcctcgcgga
      421 cgctgccatc ggaaagcgga atacgggcgg aaagtaaagt cgcaccccag tccatcagcg
      481 tgactaccat ccctgcgttg ttacgcaaag ttaacagtcg gtacggctga ccatcgggtg
      541 ccagtgcggg agtttcgttc agcactgtcc tgctccttgt gatggtttac aaacgtaaaa
      601 agtctcttta atacctgttt ttgcttcata ttgttcagcg acagcttgct gtacggcagg
      661 caccagctct tccgggaaca acgcgacgat acagccacca aatccgccgc cggtcatgcg
      721 tacgccacct ttgtcgccaa tcacagcttt gacgatttct accagagtgt caatttgcgg
      781 tacggtgatt tcgaaatcat cgcgcataga ggcatgagac tccgccatca actcgcccat
      841 acgtttcagg tcgccttgct ccagtgcgct ggcagcttca acggtgcggg cgttttcagt
      901 caggatatga cgcacgcgtt tcgccacgat tgggtccagt tcatgcgcaa cagcgttgaa
      961 ctcttcaatg gtgacatcgc gcagggctgg ctgctggaag aaacgcgcac cggtttcgca
     1021 ctgttcacga cgggtgttgt attcgctgcc aaccagggta cgtttgaagt tactgttgat
     1081 gatgacgaca gccacacctt tgggcatgga aactgctttg gtccccagtg agcggcagtc
     1141 aatcagcaag gcatgatctt tcttgccgag tgcggaaatc agttgatcca tgattccaca
     1201 gttacagcct acaaactggt tttctgcttc ctgaccatta agcgcgattt gtgcgccgtc
     1261 cagcggcaga tgataaagct gctgcaatac ggttccgacc gcgacttcca gtgaagcgga
     1321 agaacttaac cctgcaccct gcggcacatt gccgctgatc accatgtcca caccgccgaa
     1381 gctgttgtta cgcagttgca gatgtttcac cacgccacga acgtagttag cccattgata
     1441 gttttcatgc gcgacaatgg gcgcatcgag ggaaaactcg tcgagctgat tttcataatc
     1501 ggctgccatt acgcgaactt tacggtcatc gcgtggcgca cagctgatca cggtttgata
     1561 atcaatcgcg cagggcagaa cgaaaccgtc gttgtagtcg gtgtgttcac caatcaaatt
     1621 cacgcggcca ggcgcctgaa tggtgtgagt ggcagggtag ccaaatgcgt tggcaaacag
     1681 agattgtgtt ttttctttca gactcatttc ttacactcnn nnnnnnnnac tccggattcg
     1741 cgaaaatgga tatcgctgac tgcgcgcaaa cgctctgctg cctgttctgc ggtcaggtct
     1801 cgctgggttt ctgccagcat ttcataacca accataaatt tacgtacggt ggcggagcgc
     1861 aacagaggcg gataaaagtg cgcgtgcagc tgccagtgtt gattctcttc gccattaaat
     1921 ggcgcgccgt gccagcccat agagtagggg aaggagcact ggaagaggtt gtcataacga
     1981 ctggtcagct ttttcaacgc cagtgccaaa tcgctgcgct gggcgtcggt caaatcggtg
     2041 atccgcaaaa cgtgggcttt gggcagcagt agcgtttcga acggccaggc agcccagtaa
     2101 ggtacaacgg ctaaccagtg ttcggtttcg acaacggtac ggctaccgtc tgccagctcg
     2161 cgctgaacat aatccaccag cattggcgat ttctgttcgg cgaaatattc tttttgcaga
     2221 cggtcttcgc gctcagcttc gttaggnnnn nnnnnncaaa gacctgcacc catgggtacg
     2281 tttcccccag ctctgcggtt tgctcctgcc aggttttgac gatttccgtc aatgccgcaa
     2341 cgctcagttc tggcaacgtt ttactgtgat ccggtgaaaa gcagatcacc cggctggtac
     2401 cgcgcgcgct ctggcaacgc attagcggat cgttgctttc tggcgcatct ggcgtgtcag
     2461 acatcaaggc cgcaaagtca ttagtgaaaa cgtaagtccc ggtgtaatcg gggtttttat
     2521 cgcctgtcac ccgcacatta cctgcgcaga ggaagcaatc tggatcgtgc gcaggtaaca
     2581 cctgtttggc tggcgtttcc tgcgccccct gccaggggcg cttagcgcgg tgcggtgaaa
     2641 ccagaatcca ttgcccggtg agcgggttgt agcggcgatg tggatgatca ac
//
LOCUS       DAWDRC010000556         2691 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14235_length_2691_cov_2.791351, whole genome shotgun sequence.
ACCESSION   DAWDRC010000556 DAWDRC010000000
VERSION     DAWDRC010000556.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14235_length_2691_cov_2.791351"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          644 a          632 c          725 g          690 t
ORIGIN      
        1 ttttgagcgc ctcgatctgc aagccaaagg caattacacc agcgaaaaag cgatcgtggc
       61 gttgtttgac catcagcaac gcattggtga attaacgccg gagcggcgtt tttacgaagc
      121 acgtcgtcag caaatgatgg aaccgtcaat tcgctggaat ggcatccatg actggtatgc
      181 ggtcatgggc gagaaaaccg gagcggatcg ttacgctttt cgtttgtatg tacaaagcgg
      241 tgtgcgctgg atctgggggg gaggattgtt gatgatttcg ggcgcattgt taagcggatg
      301 gcggggaagg aagcgcgatg aataaagggc ttctcacgct actgctgtta tttacctgtt
      361 ttgcgcgcgc tcaggttgta gacacctggc aattcgccaa tccgcaacaa cagcaacagg
      421 cgttaaatat tgccagccag ttacgttgtc cgcagtgcca gaatcagaac ttactggaat
      481 ccaatgcgcc ggtggcagtc agtatgcgcc atcaggttta caccatggtg gaagagggga
      541 aaagtgaagt cgaaatcatt gactggatga ccgaacgcta cggagatttt gttcgctata
      601 acccaccgtt aacgggtcag acgctggtgt tgtgggcgct gccagtagtg ttgttactgc
      661 tgatggcact gatcctctgg cgagtgaggg cgaagcgatg aaacagcccc aaataccggt
      721 gaaaatgctg acaacactca cttttttgct ggtatttcac tggttcggca tttatttttt
      781 aagtccaaaa tggcaggctg tacgtgcgga gtatcagcgt cagcgcgatc cgctgcatca
      841 gtttgccagc cagcaaaccc cggaagcgca gcttcaggca ttgcaggata aaatccgtgc
      901 taatccacaa aacagcgaac agtgggcgtt actgggcgag tattatctgt ggcaaaacga
      961 ttacagcaat tcgctgctgg cgtaccgtca ggcgttgcaa ctacgtggtg agaacgctga
     1021 actgtatgcg gcgctggcga cggtgctgta ttaccaggcc agccaacata tgaccgtcca
     1081 ggctcgcgca atgatcgaca aagccctcgc gctggactct aacgaaatca ccgctctgat
     1141 gctgctggct tccgatgcgt ttatgcaggc gaattacgcg caagccatcg aactatggca
     1201 aaaagtgatg gatctcaact caccccggat caatcggaca cagctggtcg agtcgattaa
     1261 tatggcgaaa ttgttgcagc ggagatcaga ttaatcatct ggtgatgaag attatgttat
     1321 tgagctgcat aaaaaataat cgaatgaaca tataatcaaa aataatcacc aatcagtatt
     1381 attgcagatt aaacaaataa aaatccttct ataacaaatg gttattcatt aatcctgcca
     1441 taaactcgcg ttattatgca ttaatgcagc gaaaagctct gttgttaaag ggttgcgcaa
     1501 cataccgcgc aaatgatact gatcataagc gttaaaaaaa tctacaaacc aacgcaacac
     1561 aattcatgcc ctggcagtat gtcacgttct cgcgtttctg aacggggaac ggcgctccat
     1621 tgaggaagtt attctgatga aaaatataaa attcagcctg gcctggcaga ttctgtttgc
     1681 tatggtgctg ggcattctcc tgggaagcta cctgcactac catagcgaca gccgcgactg
     1741 gctggtcgtc aatttgctct ctccggcggg tgatatcttc atccatctga tcaaaatgat
     1801 tgttgtgccg attgtgatct ccacgctggt ggtgggtatc gcgggtgttg gtgatgccaa
     1861 acagctcggg cgtattggtg cgaaaaccat tatctacttc gaggtgatca ccaccgtcgc
     1921 catcattttg gggatcactc tggcgaacgt cttccagccc ggtgccgggg tggatatgtc
     1981 gcagctggcg accgtcgata tctcgaaata tcagagcact acggaagcgg tacaaagcag
     2041 ttcccacggc attatgggca cgattttgtc gctggtgccg acgaacatcg tggcgtcgat
     2101 ggcgaaaggc gaaatgctgc cgatcatctt cttctcggtg ctgtttggtc tggggctttc
     2161 atccctgccc gcgacgcatc gtgaaccgct ggtgaccgtt ttccgctcca tctctgaaac
     2221 catgtttaaa gtgactcaca tggtgatgcg ttatgcaccg gtgggtgtgt ttgcgctgat
     2281 tgcggtgacg gtggctaact ttggtttctc gtctctgtgg ccactggcga aactggtgct
     2341 gctggtgcat tttgccattc tgttcttcgc gctggtagtg ctgggaattg tggcgcgcct
     2401 gtgcgggtta agcgtctgga tcctgattcg tattctgaaa gatgagctga ttctggcgta
     2461 ctccactgcc agctctgaaa gcgtgctgcc gcgaattatt gagaagatgg aagcctacgg
     2521 agcaccggcg tcgatcacca gtttcgtggt gccgaccggt tactctttta accttgatgg
     2581 ttcgacgctg tatcaaagta ttgccgctat tttcatcgct cagctgtatg gcattgacct
     2641 gtccatctgg caggaaatca ttctggtgct gacgctgatg gtgacctcga a
//
LOCUS       DAWDRC010000557         2683 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14289_length_2683_cov_1.779680, whole genome shotgun sequence.
ACCESSION   DAWDRC010000557 DAWDRC010000000
VERSION     DAWDRC010000557.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14289_length_2683_cov_1.779680"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          680 a          633 c          660 g          710 t
ORIGIN      
        1 cgcgcatgag gcaggatgag gatcgtgcgg ctccctggtt tgtctggatg actaaactcg
       61 aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact ctcgaacctt
      121 tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta ctcaccagtt
      181 ctgcagctgc gctggcggca ccgcaggtga ttaccgtcag ccgttttgaa gtgggtaaag
      241 acaaatgggc gtttaatcgc gaagaggtga tgctgacttg ccgaccgggt aatgctttgt
      301 atgtcattaa cccaagtact ctcgtgcagt atcctttaaa cgatatcgca caaaaggaag
      361 ttgccagtgg gaagactaaa gcccaaccca tttcggtgat tcagattgat gatcctaaca
      421 atcccggcga aaaaatgagt ctggcaccgt ttatagagcg agctgaaaaa ctctgttaat
      481 tacctaaaat agccttttga tttccaataa aaaaccgcct cagttctttc accagaacgg
      541 gcggttttta acatttaagc tgatgaccac cacgcttttt attgaccatt ttgcacgcaa
      601 actggaaaac ctggcgtcgt catctattct taaagagcaa ggcaactaag cctgcattaa
      661 tgccaacttt tagcgcacgg ctctctccca agagccattt ccctggaccg aatacaggaa
      721 tcgtgttcgg tctcttttta tctgttaaaa gccagaagca ttcccttcgc tgactttata
      781 gtcaaccata acacacactc tactgtttga gtccagcgtt ttttaacatt cttgttaaga
      841 ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc cgtttatcag
      901 agaattgcgg gtcatcaatg gcgacatatc tggctttcag gcgatataca cggttgtctt
      961 gagcaattgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga tttacttatc
     1021 tcagtgggag acgttatcga tcgtgggccg caaagtttac gttgcctgca gttactggaa
     1081 caacattggg ttcgtgcggt aagaggcaat catgaacaga tggcgatgga tgcgctggca
     1141 tcccggcaga tgtctttatg gttgatgaat ggcggcgact ggtttattgc gctggcagat
     1201 aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc agcatttgcc ctttattctt
     1261 gaagtacaca gccgcaccgg caaacatgtt attgctcatg ccgattatcc agatgatgtt
     1321 tatgaatggc aaaaggacgt cgatttgcat caggtcttgt ggagccgctc gcgattaggt
     1381 gaacgccaaa aagggcaggg aattgcaggt gctgatcatt tctggtttgg tcatacacct
     1441 ttgcgccatc gcgtggatat tggcaacctg cattatattg atactggtgc tgtctttggg
     1501 ggagaactga ctcttgtgca attgcaataa ttaaaaatca ccgtactcct gtgcaggtcg
     1561 ccagaaacca tctataaaat cctcaatcgg aaaacaaccg ccatggcgga tccgttgatc
     1621 gctcatagaa taaagacact gctgttccgt gttgtagaca tccacaacaa tatcttcaca
     1681 accgccatcc aggtagcaaa caaaaagtac cagcgcgaac atttcatccc cgaagtgtgg
     1741 tgccgtaccg ttaagtttag gagagatttt acaacggggg aataacccgg acaaataacc
     1801 cgccatgccg acgggttctt tttggatcag gcaagacgca taatccaggc atcagattgt
     1861 tgatcgtaac gggtacggta aaggacagag tgttcgccat ttagcagggc agggatactg
     1921 gtttcagcat cccaggcatc cagttgcata cataaatcgg ggtctgattt acaaggaatg
     1981 gttaacgttc gatcacccgg cgattcgccg tgtaattcgc cgtcgtcgat ttcaaaagcg
     2041 cctatgcgca cactggtttt catcttcgtg ctctccattt agctgcctgt tgtggtacga
     2101 ccagtccgat cgcaccattt ttcagcctag ttgaggcagt cgaatgtgcc agtcaaaaag
     2161 tgcgcttttt tttgtgcgct gtcaggcgtt accggtaaaa agtttcccgt cgcgcaccag
     2221 ttcacgcggg tagctgtttt tcagtcgtga accaatccgt ttggctaaac caatcggctg
     2281 atgctggaag gtaaccaata catcatccgc cactggcgcg gcatgcgggt aaacatcccg
     2341 cccgcgatac cactcctccg cttcctgcgg tgtcagttca aaagcgttca cattgtcggg
     2401 ggaggcaagg gcaataacgg cttcatgctg ccagcgataa cctttgttgt gcgtttcggc
     2461 aagtttaatc cccaagcgag aaaatcggac tttaccgatc agggcttcaa tgcccaccgg
     2521 gaacaaccac agttctttgt cacgctgcca gagtcgcagg ttttcatccc agtttaagcc
     2581 aacacttgca gccgcctgac gaatttgacc ggcttcgcga tctttcactg ggctaaacgg
     2641 gaaattgccc actttgtatt ttggggtggg taaggcggga atc
//
LOCUS       DAWDRC010000558         2681 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14301_length_2681_cov_2.231531, whole genome shotgun sequence.
ACCESSION   DAWDRC010000558 DAWDRC010000000
VERSION     DAWDRC010000558.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14301_length_2681_cov_2.231531"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    309..318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          631 a          709 c          760 g          571 t
ORIGIN      
        1 acgctggaag cggcaggcga taaattgcag gccaacctgt tacgcattca ggatgcgacg
       61 atgacccatg acgacctgca ttttgtcgat cgtctggtgt tcgatcttca gagcaaactc
      121 gaccgtatta tcagttgggg ccagcaatcc atcgacttgt ggattggcta cgaccgccac
      181 gtacacaaat ttattcgtac tgcgatcgat atggataaaa accgcgtctt tgctcagcgg
      241 ttacgtcagt cggtacaaac ctattttgat gagccgtggg cgctaaccta tgccaatgcc
      301 gatcgtctnn nnnnnnnnca gtcggctccc gcggcggtgg tcgagaagga gcgtgccaag
      361 aaggccgacg ccgaggcgaa gatcgccgcg atccgcgaac agctggcggc gatcatcgaa
      421 gaacaacttg ccgtgtacaa aaccagacaa gtgccgctgg atcttggtct ggtggtacgc
      481 gaatatctgt cacagtatcc gcgtgcacgt cactttgacg ttgcgcgcat tgttattgat
      541 caggcggtac gtcttggcgt agcgcaagca gatttcaccg gactgccagc gaaatggcag
      601 ccgattaatg attacggagc caaggtacag gcgcatgtca tcgacaaata ttgaacaagt
      661 gatgccggtt aagctggcgc aggcgctggc gaatccgtta tttccggcgc tggatagcgc
      721 cttacgttca ggccgtcata ttggcctcga cgaactggat aatcatgcat tcctgatgga
      781 ttttcaggaa tatctggaag agttttacgc gcgttataac gttgagctta ttcgtgcacc
      841 ggaagggttc ttctatctac gcccacgttc caccacgctg atccctcgtt ccgtcttgtc
      901 ggaactggat atgatggtcg ggaaaatcct ctgttatctc tatctcagcc cggaacggct
      961 ggcgaatgag gggattttta ctcaacagga actgtacgac gaactgctca ccctggccga
     1021 tgaagcgaaa ctgctgaaac tggtgaacaa ccgttcaacc ggttcagacg ttgaccgtca
     1081 gaagttgcag gaaaaagtac gttcttcgct caaccgtctg cgtcgtttag gcatggtgtg
     1141 gtttatgggt cacgacagca gcaagttccg cattaccgaa tcggtgttcc gcttcggagc
     1201 cgatgtgcgt gctggcgacg atccccgtga agcacagcgt cgcctgattc gtgatggtga
     1261 agcaatgccg attgaaaacc atctgcaact caacgatgaa accgaagaga gtcagccaga
     1321 tagcggagag gaagaataat gattgaacgc ggtaaatttc gctcactgac gctgattaac
     1381 tggaacggct tttttgcccg aacttttgac cttgacgagc tggtcacgac gctttctggc
     1441 ggtaacgggg cgggtaaatc caccaccatg gcggcgttcg ttacggcgct tatccccgac
     1501 ctgaccctgc tgcatttccg taacactacg gaagccgggg ccaccagcgg ttcgcgcgat
     1561 aaaggtctgc acggtaagct gaaagcgggt gtctgttatt cgatgctcga caccattaac
     1621 tcgcgccacc agcgtgtagt ggtcggtgtg cgtctgcaac aggttgccgg acgcgatcgt
     1681 aaagtggata tcaaaccatt tgccattcag ggcctgccga tgtcggtgca gccgacacaa
     1741 ctggtgaccg aaactctgaa cgaacgccag gcgcgcgtgc tgccgcttaa cgagctgaaa
     1801 gacaagctcg aggcgatgga aggcgtgcag tttaaacagt tcaactccat taccgattac
     1861 cactcgctga tgttcgatct gggcatcatc gcgcgtcgtc tgcgctcagc gtctgaccgt
     1921 agtaaattct atcgtctgat cgaagcctcg ctgtatggcg ggatctccag cgccattact
     1981 cgttctctgc gcgactacct gttgccggaa aacagtgggg tgcggaaagc gttccaggac
     2041 atggaagcgg cgctgcgtga aaaccgcatg acgctggaag cgattcgtgt cacccagtcg
     2101 gaccgtgacc tgtttaagca tctgatcagc gaagccacca actacgtggc ggcggactac
     2161 atgcgtcacg ccaacgagcg ccgtgttcat ctcgataagg ccctggagtt tcgtcgcgag
     2221 ctacatactt cgcgtcagca actggcggct gagcagtaca aacacgtcga catggcgcga
     2281 gagctggctg agcacaacgg tgccgaaggc gatctggaag cggattatca ggcggccagc
     2341 gatcacctga acctggtgca aaccgcactg cgtcagcagg agaagatcga acgctacgaa
     2401 gcggatctcg atgagctgca gatccgtctg gaagagcaaa acgaagtggt ggcagaagcc
     2461 atcgaacgcc aggaagagaa cgaggctcgt gcggaagctg ccgaactgga agtggacgag
     2521 ctgaaaagcc agcttgctga ctaccagcag gcgctggacg tccagcaaac gcgcgcgatc
     2581 cagtataacc aggcgattgc tgcgcttaat cgtgccaaag aactgtgcca tctgccggac
     2641 ttaaccgccg acagcgctgc cgaatggctg gaaaccttcc a
//
LOCUS       DAWDRC010000559         2675 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14337_length_2675_cov_1.917557, whole genome shotgun sequence.
ACCESSION   DAWDRC010000559 DAWDRC010000000
VERSION     DAWDRC010000559.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14337_length_2675_cov_1.917557"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2402..2411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          633 a          672 c          771 g          589 t
ORIGIN      
        1 aaatccagca ggcggtaatt actcagatgc tgcaggcacc gcaaacgctc ggcgaagaag
       61 catcgaagtt aagtaaagat ttcgatcgcg gcaatatgcg cttcgattcg cgtgataaaa
      121 tcgtggccca gataaaactg ctgacgccgc aaaaacttgc tgatttcttc catcaggcgg
      181 tggtcgagcc gcaaggtatg gctattctgt cgcagatttc cggcagccag aacgggaaag
      241 ccgaatatgt gcatcctgaa ggctggaaag tgtgggagaa cgtcagcgcg ttgcagcaaa
      301 caatgcccct gatgagtgaa aagaatgagt gatgtcgccg agacactaga tcctttgcgc
      361 ttgcccttac agggcgagcg cctgattgaa gcctctgccg gcacaggcaa aacctttacg
      421 attgcggcgc tctatttgcg cctgttactt ggactaggcg gttctgccgc ctttccccgc
      481 ccgctgaccg ttgaagaact gctggtggtg acctttaccg aggctgctac ggcagaattg
      541 cgtggtcgta tccgtagcaa tattcacgag ttgcgtatcg cctgcctgcg tgaaaccact
      601 gacaatccgc tgtatgcacg tctgctggaa gagatcgacg ataaagcgca agccgcgcag
      661 tggttgttgt tagccgaacg gcagatggat gaagcggcag tctttactat tcacggtttt
      721 tgccagcgca tgctcaacct gaatgccttt gaatccggca tgctgtttga gcagcagctg
      781 attgaagatg agtctctgct acgctaccag gcctgcgccg atttctggcg tcgccactgc
      841 tacccgctga cgcgtgaaat tgcccaggtg gtctttgaaa cctggaaagg gccgcaggcg
      901 ttgctgcgcg atattaatcg ttatctgcaa ggcgaagcac cggttatcaa agcaccgccg
      961 cccgatgatg aaacgctggc ttcccgccac gcgcaaattg tggcgcgtat cgacgccgta
     1021 aaacagcagt ggcgcgacgc ggtgggtgaa ttggatgcgc tgatcgaatc ttctggtatt
     1081 gatcgacgca agtttaaccg tagcaatcag gctaaatgga tcgacaagat cagcgcctgg
     1141 gcagaagaag agaccaacag ttatcagttg ccagagtcgc tggaaaaatt ctctcagcgt
     1201 ttcttagaag atcgcacgaa agccgggggg gaaaccccgc gacatccact gtttgaggcg
     1261 atcgatcaac tgcttgcaga accattgtcg atccgcgatc tggtgatcac ccgcgcattg
     1321 gctgagatcc gcgaaacagt agcgcgtgaa aaacgccgcc gtggcgaatt gggttttgat
     1381 gacatgttaa gtcggctcga ttccgcgctg cgtagcgaaa gcggcgaggt gttggcagcg
     1441 gcgatccgta cgcgattccc ggtggcaatg atcgatgaat ttcaggatac cgacccccag
     1501 cagtaccgaa tttttcgccg tatctggcac catcagccgg aaaccgcatt gttgctgatt
     1561 ggcgacccga agcaggccat atatgcattc cggggtgcgg atatcttcac ttatatgaag
     1621 gcgcgtagcg aagttcacgc ccactacacc ttagacacca actggcgttc cgcaccggga
     1681 atggtgaata gtgtgaacaa gcttttcagc cagactgatg acgcgttcat gtttcgcgaa
     1741 ataccgttta ttccagtgaa atcagccggg aaaaatcagg cgttacgttt tgtgtttaaa
     1801 ggtgaaacgc agcctgcgat gaaaatgtgg ctgatggaag gcgaaagctg cggcgttggc
     1861 gactatcaaa gtaccatggc gcaggtatgt gctgcgcaaa tccgcgactg gttacaggct
     1921 gggcagcgcg gggaagcgtt gctgatgaac ggcgatgatg cacgtccggt acgtgcttcg
     1981 gacatcagtg tgctggtgcg cagccgccag gaggccgccc aggtgcgcga tgccttaacg
     2041 ttgctggaaa tcccttccgt ttacctttcg aaccgcgaca gtgtttttga aactctggaa
     2101 gcgcaggaaa tgctttggtt gttgcaggcg gtgatgacgc ccgaacgaga gaacaccctg
     2161 cgtagtgcgc tggcaacgtc aatgatgggg ctgaacgcgc tggatatcga aacgctgaac
     2221 aatgacgaac atgcgtggga tgcggtagtc gaagagttcg atggttatcg gcaaatctgg
     2281 cgcaaacgtg gcgttatgcc gatgctgcgg gcgctgatgt cggcgcgtaa cattgctgaa
     2341 aacttgctgg caacggcagg cggtgagcgg cgtcttaccg atatcttgca tatcagcgaa
     2401 cnnnnnnnnn ngcgaactgc tacaagaagc cggaacgcag ctggaaagtg aacatgcgct
     2461 ggtacgctgg ttatcgcaac atatcctcga gccagacagt aatgcctcca gccaacaaat
     2521 gcgcctcgaa agtgataaac atctggtgca gattgtcacg atccacaaat caaaagggct
     2581 ggaatatccg ctggtctggt tgccatttat taccaatttc cgcgtccagg atcaggcgtt
     2641 ctatcacgat cgccactcgt ttgaggccgt tctgg
//
LOCUS       DAWDRC010000560         2675 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14338_length_2675_cov_1.842366, whole genome shotgun sequence.
ACCESSION   DAWDRC010000560 DAWDRC010000000
VERSION     DAWDRC010000560.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14338_length_2675_cov_1.842366"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1263..1272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          561 a          719 c          806 g          579 t
ORIGIN      
        1 cccgccgtcc ggcactgatc ttaatggacg aactggcgca cagtaatgcg ccaggttctc
       61 gtcatcctaa acgctggcag gacatcgaag agctgctgga agcaggcatt gatgttttca
      121 ctaccgtcaa cgttcagcat ctggaaagcc tgaacgatgt ggtcagcggc gtcaccggaa
      181 ttcaggtacg ggagaccgtg cccgatcctt tttttgatgc cgccgacgac gtggtgctgg
      241 tggacttgcc cccggacgat ctgcgccagc gactgaaaga aggcaaagtc tatattgccg
      301 ggcaggcgga gcgcgccatt gaacattttt ttcgcaaagg caatctgatc gccctgcgcg
      361 aactggcact gcgccgtact gccgatcgcg tagatgggca aatgcgcgcc tggcgggggc
      421 atcctggcga agagaaagtc tggcacacac gcgacgcgat ccttttatgc atcgggcata
      481 acaccggcag tgagaaactg gtccgcgcgg cggcgcggtt ggcctcgcgc ctgggcagtg
      541 tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg cttaccggag aaaaaacgtc
      601 gggcaattct cagcgcctta cgtctggcgc aggagctggg cgcggagacg gcaacacttt
      661 ctgatccagc ggaagagaaa gcggtagtgc gttatgcccg tgaacataat ctcggcaaga
      721 ttattctcgg tcgcccggct tcgcgccgct ggtggcgtcg ggaaacattt gctgaccggc
      781 tggcacgcat cgcccccgat ctcgatcagg tgctggtcgc gcttgatgaa ccacccgccc
      841 gcacgattaa caacgcgccg gatagccgct cttttaaaga caagtggcgg gtacagattc
      901 agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac cttaattgcc atgcagtggc
      961 tgatggcgtt tgatgccgcc aacctggtga tgctgtatct gcttggcgtc gtggtggtgg
     1021 cactatttta cggacgctgg ccttcggtgg ttgccaccgt cattaatgtg gtgagtttcg
     1081 atctcttttt tatcgcccca cgcggcacgc tcgccgtctc tgatgtgcaa tatctgctga
     1141 ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa ccttactgct ggcgtgcgtt
     1201 atcaggcgcg ggtagcccgt taccgcgaac aacgcacacg gcacttatat gaaatgtcca
     1261 aannnnnnnn nnttcgcagg atatcgccgc caccagcgaa cagtttattg cctccacgtt
     1321 tcatgcccgt agccaggtgt tgctgcctga tgacaacggt aaattgcagc ctttaacaca
     1381 tccgcaagga atgacgccgt gggacgatgc catcgcgcag tggagttttg ataaaggcct
     1441 gcctgcgggc gcgggcaccg acacgttacc cggtgtaccg taccagattt tgccgctaaa
     1501 aagcggcgag aaaacctacg gactggtggt ggtggaaccg gggaatctgc gccagttgat
     1561 gatcccggaa cagcagcgcc tgctggagac gtttacgctg ttagtcgcca atgcccttga
     1621 gcgactgaca ctgaccgcca gcgaagaaca ggcgcggatg gcaagcgaac gtgaacagat
     1681 ccgcaacgcc ctgctggcgg cgctttcgca tgatttacgc acgccgctta cggtgctgtt
     1741 cggtcaggca gaaatcttaa cgctcgatct ggcaagcgaa ggatcgcccc acgcccgcca
     1801 ggccagcgag attcgtcagc atgtgctgaa caccacccga ctggtgaata atctactgga
     1861 tatggcgcga attcagtccg gcggctttaa tttgaagaaa gagtggttaa cgctggaaga
     1921 agtggtcggc agcgcgctgc aaatgctgga accgggttta tcgtcgccca tcaatctttc
     1981 tctgccagag ccgctgacct taatccacgt tgacgggcca ctctttgaac gggtgctgat
     2041 taacctgctg gagaacgcgg tgaaatatgc gggggcgcag gccgaaattg gtatcaatgc
     2101 ccacgttgag ggcgaaaatc tacaactgga tgtctgggat aacggccccg gtcttccacc
     2161 aggccaggag cagacgatat ttgataagtt tgctcgcggg aataaagagt cggcggtacc
     2221 gggtgtaggg cttgggctgg caatttgtcg ggcgatagtg gatgtacacg gcggcaccat
     2281 taccgcattc aaccgaccgg aaggtggtgc ctgttttcgt gttacacttc cccagcaaac
     2341 agcccctgaa cttgaagaat ttcatgagga tatgtgacaa acgttctgat tgttgaagat
     2401 gaacaggcta ttcgtcgctt tctgcgcacg gcgctggagg gcgacggtat gcgcgtcttt
     2461 gaggccgaaa cgctgcaacg cggcttgctg gaagcggcaa cccgtaagcc agatttgatt
     2521 attctcgatc tcggcctgcc cgacggtgat gggattgagt ttatccgcga cctgcgccag
     2581 tggagcgcgg tgccggtgat tgtgctttcc gcacgcagcg aagagagcga taaaatcgcc
     2641 gcgctggatg ccggagcgga tgattatctg agtaa
//
LOCUS       DAWDRC010000561         2672 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14356_length_2672_cov_1.836836, whole genome shotgun sequence.
ACCESSION   DAWDRC010000561 DAWDRC010000000
VERSION     DAWDRC010000561.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14356_length_2672_cov_1.836836"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    292..301
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    547..556
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          680 a          716 c          646 g          610 t
ORIGIN      
        1 caccggcgaa atgagcggct tgccgacgat caacaccgcg agcgccacca gaataccgtt
       61 tccgcccgcc agtaaaccgg acttcacgcc taacaaccac ggtctggtgg tgcgtggatc
      121 taaacgcatc acctctgggt aaaaactttt gcccagtaaa cccgctggcg ttccggcggc
      181 atcgaagaat gtcatggcga ttttaaataa cccggcggca gcgggtccta acacgatccc
      241 caccaacact gtgctgcacg agttacgcgc cgaccagatg gagtgggcaa tnnnnnnnnn
      301 naccagacaa aactccacgc gcctttaatg tgtcgggcag actcaaacag attcaatttg
      361 aaggcgttat ggatatttct gcggcgtaat tcgcgcgcgg caaaccacca gtacatggtg
      421 ccgccaacca gattcgacac gtaccaggca ataacaaaac ccgcaaaacc aaagtcaaaa
      481 taccaggcta cgacgctccc cgctgcgcgc agaaaaggtt tcgtcgcctg ctgtacagca
      541 atcaaannnn nnnnnnacgc agaatgccgg tcggcgtgga ggaagccatt gaaggaatga
      601 gcgtgcaata gagcgccgcc agccaaaagc tttggtcatc cagacctaat gaatgggaaa
      661 ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg atatccagcg
      721 agaaggaaaa tgagacaaca ttgcgaaatt gctgcggatt attgttggtc aaggccggcg
      781 ttccgtactg aaccaccagt tgccatgtct gaaacttaat aaaatcgctg atcgatttgg
      841 cgtacgattg cacaattacc agtacgccaa acatggcggg cgtcatccct ttaccggcac
      901 acgagagcgc caacagcccc agcaaagcgc tcacaacatt actggagcct aaccaggcgc
      961 tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg ttaaaacccg
     1021 ccaattcagc ctgaccttat cgttgatagt aaaaaatatc ccgccagcct taagttaaac
     1081 ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt tccagcacct
     1141 cgctgtaagg cgctctggaa tcaatctcaa gaatttttgt gccgttatag ccaatcgtcg
     1201 acatgacgcc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg cgggagatgg
     1261 cagtatcaat atcaatcccc aagcgaatga ttaattcagg acggtattgc gccatttttt
     1321 ggtataaacg ccgttcacgc tgcgccagaa acatgctgat tttcccggtc gcacgttcga
     1381 cgccgatccc cggtccgtca taataaaagc ccgaaatttc agcctgcggg aagcgatcgc
     1441 tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgcagattc gccattcttc
     1501 gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg gttttcatgc
     1561 tttgggtttt cgaggatttg gccgccagtc gccgttcgag ccagacacca accaacggca
     1621 atcgtttgat tttgtcgccg tcttcgccgg agagcagccc cagatagcgc cgctcggttt
     1681 gccagtgttg ttgcagcgat ttcaccaggt catttgtcag tgtggattta ccggttccat
     1741 cacacccgac caccgcaatc agccccggaa tgtagttggg ctgcggcgcg gttgtactct
     1801 taacagtttg tgtttgtagt gcatccattc gtttaatcat cccctatcca ttttggtgat
     1861 caggaaagcg cggcgtgaat tgcgagtagt gacgcttgca gaatttcatc ggctggttgc
     1921 cgaccatcca gttcaagaat cctggcacca ttgaatgtca gttgcggcgt aacggcgatt
     1981 ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt ttgttcatca
     2041 atgccaagac gaatcaacaa tacaggcaaa taagaagcca tccattggta cagcttcagc
     2101 tcacgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc caattgcggg
     2161 ccatcaaagc ggaaacccgg cacttcaacc tgcgggtagc ggtcggtgat gagcagatag
     2221 ccttgctggt ttttacacaa cattttgcga aacttgtacg cccgccagca ggaaagcaga
     2281 tagataacca gcgcagtaat attgccaggc ggtgttgagg gcttttcgtg cacatgtgcc
     2341 gctttacttc gcaaataacg cccaaaaggt gcgccaataa caggtagctg tgaaatccat
     2401 tcgccaattc gcccggacga ttgcccgaga taaatgtgtt ctgttggcat tcttgatgcc
     2461 agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca tcctacaatg
     2521 gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt cacgaaaaac
     2581 ccggcagcaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc cgttcaacct
     2641 tattgtgaca atggttaatt gtgacattga cc
//
LOCUS       DAWDRC010000562         2664 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14401_length_2664_cov_3.047144, whole genome shotgun sequence.
ACCESSION   DAWDRC010000562 DAWDRC010000000
VERSION     DAWDRC010000562.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14401_length_2664_cov_3.047144"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2054..2063
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          696 a          688 c          637 g          633 t
ORIGIN      
        1 ggcggttggg tatgcgtctg atctattggt gcgctgcggc gcaacggtga tgttctccga
       61 agtcactgat gtacacgacg ccattcatct gttaacgccg cgcgccatca atgaagaagt
      121 gggcaggtgt ctgctcgaag agatggcctg atacgataac tatcccgata tgggcaaaac
      181 cgaccgcagc gccaaccctt cgcggggcaa caaaaagggc ggcctcgcca acgtggtaaa
      241 gaaggcactc ggctccattg ctaaatcggg taaaagtgca attgttgaag tgctgtcgcc
      301 cggccaacgc ccgactaaac gcggattaat ttacgccgcg acgccagcca gcgattttgt
      361 ctgtggcacg caacaggtgg cttcgggtat caccgtgcaa gtgtttacga ccggtcgtgg
      421 tacgccgtac ggcctgatgg cggtacccgt cattaaaatg gcgacccgca ccgagctggc
      481 gaaccgctgg tttgatttaa tggatattaa cgcgggcact atcgctaccg gcgaagaaac
      541 cattgaagaa gtgggctgga agttgttcca ctttattctc gacgtcgcca gcgggaagaa
      601 gaaaactttc tcggatcaat ggggactgca taaccagctg gcggtgttta acccggcacc
      661 ggtgacctga tttcttttgg aacgtccctc gcaaaacatg gccttagtgc catgttttta
      721 ttgtttaaag tccacacatc cattaataat gcatttgcat taccttagtt caagcttata
      781 atttgagcag aaaacaggct gtaaaaggac agtaaataat gcccgctaat gctcgctcta
      841 acgctgtact gaccactgaa tcaaaggtca ctatacgcgg acaaacaact atccccgcgc
      901 cagtgcgtga ggccttaaaa ctgaagccag gcctggacag cattcattac gaaattctgc
      961 ctggtgggca agtatttatg tgccgactgg gagatgaaca ggaggatcat actatgaatg
     1021 catttttgcg ttttctggat gcagatatcc agaacaaccc gcaaaaaact cgtccattcg
     1081 acattcaaca aggaaagaaa cttgtcgctg gcatggacgt caacattgac gatgagattg
     1141 gcgacgacga ataatggatt ttccacaaag ggttaatggt tgggcgctat atgctcatcc
     1201 ctgttttcag gaaacctacg atgctttagt tgccgaagtc gaggcattaa agggaaaaga
     1261 tcctgaaaat tatcagagaa aagctgccac aaagttattg gcggtggtcc ataaagtgat
     1321 tgaggagcat atcacggtca atccatcatc accggcattc cgtcatggca agtcgttagg
     1381 ctctgggaaa aataaagact ggtcacgggt aaaatttggt gctggtcgct atcgtctctt
     1441 ctttcgttac agtgaaaaag agaaagtcat cattctggga tggatgaacg atgaaaacac
     1501 gctgcgcacc tacggtaaaa aaacagatgc ctataccgta ttcagtaaaa tgttaaaaag
     1561 aggacatcct cctgccgact gggaaagcct cacccaagag acagaagaaa gccattgatg
     1621 gtgttcacaa tgcccgccga acgcattggt gttgaccagc gggcatcctt tctcattccc
     1681 cgaccagaat cacttcaacc ccagcctttc tcagtccttc cagactatcc gcaggaatgc
     1741 cttcatcaac aatgatcatg tcgatacgtt gagtatcaat gatcttatgt aaactagaac
     1801 gattgaactt actggaatcg gtgaccacga tgatccgttc cgcaacttcg cacatccgac
     1861 ggtttaaacg ggcttcatct tcattatgcg tgctgacgcc gcgctccaga tcgatcgcat
     1921 ctacaccaag aaacagcata tcgaagtggt aattttgcag cgattgctca gcctgatcac
     1981 cgtaaaaaga ttgcgactga cggcgtcgta ctatcgcaga tcaacgcgcc accataggca
     2041 cgcacagcga tccnnnnnnn nnngcgattg ctcagcctga tcaccgtaaa aagattgcga
     2101 ctgacggcgc aaatgccccc cggtcatcag cagctcaacg ccttccgctt ccagcaacgc
     2161 attagccacg ttcataccgt tggtcatcgc aattacgtca gtgtgcttgc gcatcagacg
     2221 agcaatctca aaagtggtgg tcccggagtc gaggatcacc cgatgaccag gctgaatcaa
     2281 ctcaacggca gctttcgcaa cgctgcgttt catcgcggtg ttcagcgcgc ttttatcttc
     2341 cactgatggc tcgactgacg gcgttgtgct atcgcagatc aacgcgccac cataggcacg
     2401 cacagcgatc ccctgctttt ccagaaacgc cagatcgttg cggatcgtca cagtagatac
     2461 gccatacaat gccgacagat cgttaacctg ctcactccct tgctgtcgca gacgctgaat
     2521 gatctgttct cgtcgctcgc tggtgcctgt cactcgcttc tcacctgaag cgtcggtatt
     2581 actcatagta agtcctttcg taaaactttc gtttcatttc gttttgccta ttaacgcctt
     2641 tctattaagc aaatgcaagc ccac
//
LOCUS       DAWDRC010000563         2643 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14542_length_2643_cov_2.126352, whole genome shotgun sequence.
ACCESSION   DAWDRC010000563 DAWDRC010000000
VERSION     DAWDRC010000563.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14542_length_2643_cov_2.126352"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1030..1039
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2314..2323
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          750 a          613 c          542 g          718 t
ORIGIN      
        1 ctgttcacac tatttacctc cagcattcta tataaataat agaaataaaa accccaatac
       61 atagtgcatt ggcatgacca aacatcaatg ccattatact cgcctctgtt aaatactcgc
      121 tagttaatat gataggcacg catgatataa caatccatat gctaatacat aaatacatca
      181 gtatttttct catagtcagt ccctgtgatt aacaatcaag gcatagttaa gcatatttca
      241 cattcaaacg taaacattat taataacatt atgtcaatcg aactgacatc acgattcaag
      301 ccctcttcag actgaaggtt agagcagcgt agaacatttc aaaacaatac actaaaaaaa
      361 tacttgaaca aaaaacttaa ttctttttta ttgtgaactt tagtaatttt gtcatgcaac
      421 tctttattac atttaggcct atcttatttc atggcaacgc agatacagga ccatttcttg
      481 tgctatggat aagtaataag ctagttacat cttattaaga gaaccatttt tacatatcaa
      541 gatatcatca ccacccacaa tgaattaagt atttttctta ttctttagat tagatacgta
      601 atgaatccct aactccattc gggtgtcatg gttctttcat ttgaaacaca ttcatatcca
      661 atgtaatatt tctatgcgca aatataacta ccgaccatac tcatcattta ttacagagaa
      721 ttattttatt caccgaaaag tttcacaaaa tatcccctga tagaaaacta aaaaacactg
      781 cataatatcg ctattctggc acttgcgaaa aattatattc tgcacctgtt gtagcgttct
      841 atttctccgc ctggcaacga atgcacaatg tccgccacag tttctgttct aatttcttcc
      901 tgtgttaccg catcctgtac gggtagcgca acaccagcat gctaagcaag gctttcccat
      961 cagatttcac tacgactatt tgggagcgag atggaatgca gcagtaaaac gtgctggtat
     1021 tcgccgccgn nnnnnnnnng cgcagatggt gtatgaaatt tacggtatgt ggattgatga
     1081 catgaacgac gaacaggtag ccatgttgaa tgcgcggtta tcgtagttgc aaagtttgcc
     1141 ctcaatatgc cccatttagt accagagaac tgaaataatg caagaaaatc aacaaattac
     1201 aaagaaagaa caatacaacc tgaacaaatt acaaaaacgt ctgcgtcgta acgtgggcga
     1261 agccattgct gacttcaata tgattgaaga aggcgatcgc atcatggttt gcctctccgg
     1321 gggtaaagac agctatacca tgctggagat tctgcgcaat ttgcagcaaa gcgcgccaat
     1381 caatttttcg ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc cggaacacgt
     1441 tctgcccgag tttcttgaaa cgctgggcgt tgggtacaag attgttgaag agaacactta
     1501 cggtatcgtg aaagagaaga ttccggaggg caaaaccact tgctcactgt gttctcgtct
     1561 tcgtcgcggt atcctttatc gcaccgcaac ggaactgggg gcgacgaaga tcgcgctggg
     1621 tcaccatcgt gacgatatcc tgcaaacgct gttcttaaat atgttctacg gtggcaagat
     1681 gaaaggtatg cctccgaaac tgatgagcga tgatggcaaa catatcgtga ttcgcccgct
     1741 ggcctactgc cgcgagaaag atattcagcg ctttgccgat gcaaaagcgt tcccgattat
     1801 tccgtgcaac ctgtgcggtt cacagcctaa cctgcaacgt caggtaattg ctgacatgtt
     1861 gcgtgactgg gataaacgtt atccggggcg tatcgagacg atgttcagcg caatgcagaa
     1921 tgtggtgccg tcgcatctgt gcgataccaa cctgttcgat ttcaaaggca ttacccacgg
     1981 ttctgaagtg gttaacgggg gtgatctggc gtttgatcgc gaagagatcc cactacaacc
     2041 agcgggctgg cagcctgaag aagatgaaaa tcagttggat gagttacggc tgaatgtggt
     2101 tgaagtgaaa taacccggat agcgcccgat gcgcaagcgt atcgggctac tcttatggag
     2161 gccggataag acgcggccag cgtcacatcc ggcaatgccg aagaagatgt ttactcttgc
     2221 accctgcaat tcaacatttc attattttaa taaccgcacc cggcacgttt ttcctttaat
     2281 cttcccaccc tttagctgtt tccatgcttt atgnnnnnnn nnnaacagcc tgacgcaccg
     2341 cgacatagac atgcgccgga tgcacggcaa ttttgccaat atctgcgcca tcaagcccga
     2401 tgtctcctgt cagcgcacct aatacatcac ccgggcgcat tttggctttt ttcccgccat
     2461 cgatacacaa cgttgccatt tctgctttca gcggcacaat ggaactattt gctggcggcg
     2521 tttgccagtt aagttttatc tgcaacatgt cagaaatgat attggcccgc tgtgcttctt
     2581 ccggggcaca gaaactgatc gccagaccgc tatttcctgc acgagctgta cgaccgatgc
     2641 gat
//
LOCUS       DAWDRC010000564         2638 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14574_length_2638_cov_1.612079, whole genome shotgun sequence.
ACCESSION   DAWDRC010000564 DAWDRC010000000
VERSION     DAWDRC010000564.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14574_length_2638_cov_1.612079"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          550 a          749 c          799 g          540 t
ORIGIN      
        1 ccactgggcg aaaccgtcac gcggcacacc gcgctgtcat cgctgacgct caccacgtct
       61 tccagcagta acatgggcgc gtcgtggggc agataagcgc cgggggataa atagtggctc
      121 acgaaaccct cccgagcaga atgctggcgt tattgccgcc aaaggcgaag gaattggaca
      181 ggatcaccgg acgtgccagc ggctgcggtt tttcgataat gccacacggt ggcagcgtgg
      241 gatcgcgcgg cgacaggctg aagtcctgag cgggcagcgg taaatcacgc tgtaaaatca
      301 acatactaat tgctgcctcg gtaatgcccg ctgcgcccag cgtgtgtccg gtcaaatgtt
      361 tggtggagct gcacggtacg cgttcgccaa acagggcgtt aaccaccatt gattcaatct
      421 ggtcgttgag ctgtgtggcg gtgccgtgca ggttgatata gccgacgtca tccggcgtaa
      481 gctgcgcatc ggtcagcgcc tgattaatag cgcggatcgc tccttcgccc tgcggatgcg
      541 gggctgaaat atggtaagcg tcgctcgatt caccaacgcc cagcaacgcg atgggctgcg
      601 gttcccgggt cagcagcatt agccccgccc cttcaccaat ggtgatacct gctcggtcac
      661 gaccaaacgg ctgacataag gttggcgaca gcgattcgag gctgtgaaaa ccgttaatcg
      721 gcatccggct cagggtgtct gcgccaccca caatggcggc atcgaccagc cccgcttcga
      781 taagcctgcg cccgctgatt atcgctctgg cgctggaaga gcaggcggtt gaaagggtat
      841 aggccgggcc gtcgagcgcc agccagtggc tgagaaaacg cgacggatcg ccgagttcct
      901 gttgcgcgta gtgccatgtg gtgctcggct gcccgtgggt gcgcaggtta acgtgcgtgt
      961 cgccttcatg caggccggag gtgctggtgc caagtacaat cgcgatgcgc tcgcgaccat
     1021 atttcgctat agcgtcatca acctgcggct ggagctgcgc cagcgccgcc agcaggatct
     1081 gattgttgcg cgagcggtgc gcagcgaatt tttccgggat aagcggaagc tcgccatcca
     1141 cgccagccag gaccgcctgc ggatgcccct gcaaccagcc tgcgcgcggg cgcattccgg
     1201 gagcaacacc gcgagtcagg ttggttgcga tttcgtcaag gttatttccc agggcgttga
     1261 tcatgccgac ggcggaaata taaatcatat cagtcaccta agtattgaat ggtgatgtgg
     1321 tatttaaaga catgctgctc aatgctgatt ggcacgcgtt ttccctggcg attcagatag
     1381 gtgatttccg tgaccagttt gccgctggcg ttacgcagct cgcgtttgtc gccgttgtcg
     1441 cgaagcgtcc agcctgtggg aagttgcggt tgccaggcgc taatcggcca gtggctgagc
     1501 atcacgtcag ccagtacctg acttgcgggc ggtaactgtg ggacgacgat ggattgctcg
     1561 gcgcgtagcc cttttgcatc gtaggtcacc agaaacaggc gaatgccgac cgacgacagc
     1621 ccggcaaggg tgattttctg atcttcggca ttaagcatca ctagcagaga ctgggttttg
     1681 ccgttgaagc tgccggtcaa cagttgctgg gaattgactg cgggtgaaat ccccggcgca
     1741 ggcagcgtga tgcgcgtacc aggttgcagc cacgcctgcg ggcggccttc ctgttccggt
     1801 tgcgagtggc tacagccagt gaggataagc gtagcggtca gggcgagcgc ccgccagaat
     1861 gtggatttga tcattttttt gttctctttt tatcgggcat cgccagcggc gaaagcagga
     1921 aggcggtgaa aataccgctc accagcacaa tgccaaaact gctgatggcc tgggtggcgc
     1981 tgaataccag cattcccagc gtcagcaagg tggtgagcat tgccagcgtg atcgccagta
     2041 gggaagttaa cggtttaccg cgcggattac tgaaaaacag cgtgtaattg atgccgatgc
     2101 caagcaccag caccagcgcc agcagcgaaa agagattcac cgcctgcccg ctaatcgcca
     2161 gcacagccag accgcagccc agcgaaagca ccgaaggcac caggctgata aaccctttgc
     2221 gccagccgag gcgggccact gcgccgcagg caattactgc cagcgccacc agcaacaagc
     2281 cggttaacac ataacggtaa agtgcgaaca attcatcaaa ggtgtttttg cgatcaaccc
     2341 aggcaatgcc acaagggtaa tagctggaga tttcctgtag cgccgcgcta tttttaaccc
     2401 cttcaaccgg caccagcacg ccgcttgcgc cattttccag cgtcagccac agcagacgcc
     2461 agccttcact ggcggggctt gccagccatt catcaacctt caccggcatg gcgttgagat
     2521 ccgggttcac tgttgtcagc ccggcatttt gcagtgcttt tgttactgtc ggggccgctg
     2581 ttgtcagcag atgtaaatct tcctcctgcc gcgcaaggga gttcagtgga atggtgcg
//
LOCUS       DAWDRC010000565         2637 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14579_length_2637_cov_1.783501, whole genome shotgun sequence.
ACCESSION   DAWDRC010000565 DAWDRC010000000
VERSION     DAWDRC010000565.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14579_length_2637_cov_1.783501"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    391..400
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1132..1141
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1720..1729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2286..2295
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          622 a          607 c          669 g          699 t
ORIGIN      
        1 ccagccgttt ggcgcatccc attgttgccc acttttcacc gacgtggtgt tcaggccgcc
       61 tggttgcagc agatgtgttt tcgtcgccgt cgccattttg ctggcgcgat ctttcgctgc
      121 cgcattgacg tacagcggga acagggcggc cgcggttaac tgattgcgca ctttatgact
      181 tttcaggtcg taatcggcat accagctttg ttgatcgttc cacaggtatt tttcgatccc
      241 tttttgacgg gcatttgcca gcgtttcgta ctggtttgcc atcgcgttat ctccggcagc
      301 tttgctggcg cgggagagga ttttttccat tttgaacatc aagctgttca gatcgaccgg
      361 tacgatgctg gttgtgcgta aggtgtttaa nnnnnnnnnn gctgcggatt gtccatccag
      421 cgcgagctga aatcccagcc agacgcagcg gcagagcgca ggtcgcggta aatttcagtg
      481 gcaggtcgat tcggattgct tttggcagtg gcaatatctt ccacccatga ctctggtcgt
      541 ggcgtatcgc gatcgtccca gtagcggttg agaagggtac catcctgaag ttttacaacg
      601 cgtttttcct gttgtccggc ttgcaggttt tcaacaccgt ccatccagta agcatattct
      661 ttttgcattt gcggcaggta ttgtttcaac gcggcgtcgc cttcatgctg cgccagtaac
      721 tctaccatca gggcaaagaa gggcggctgc gagcggctta aatagtaact gcggttgccg
      781 ttgggaatat gaccgtaagt gtctatttca tgagcaaaat tggccaccat atccgcgact
      841 ttatcccagt gaccgctttc ggcaagtcct aacatggtga agtaactgtc ccagtaatat
      901 acctcgcgaa aacgtccgcc tggcacgaca taaggttttg gcagcggtaa tagagaatcc
      961 catttttcgg tgttttcggt agaacgcgtt aataccggcc aaagtccgtc aatatgttcg
     1021 cgcagtgatt gcccctctgg cggaacatat ttctcgccct ctttcggcag ggtgaaattg
     1081 acgttaacga aatggcgtag atcaaatccg ctctggtttt gctgcatccg annnnnnnnn
     1141 nccgataatc agcaaggatc atcagcggat cgctgttcgg cacggcatcg gcaaaggttt
     1201 tttggtccgg aaaaagtttg gcgttttgca catcattaaa cagcggccct aataaaatat
     1261 caggcggttg tggtgttacc gatgtttctt ctgcctgcac cgatagcgca gcgaaacaca
     1321 aaaagatgca ggctggaatt aacgccattt tttgcgggcg ataaggtgcg ggggatttca
     1381 tcaatcattc tcctttggcg aaaccgaata aaacggtatt caagtatcag aaaaccttag
     1441 ttcaggatcg cactagctgc atgatcgaaa tcacattctg cgaactgaca gacattttag
     1501 aaaagggaat taaagggata aataacggcg aaatagagtg ataaaataac atctgtttat
     1561 tagtcagcca gcgacgttgg gttcgcgatt agcgaagaaa aatcatcata atttggtgaa
     1621 aaatataaca ggtagttaaa aaacatcagt gcagagtaaa ttgccggatg acatcagaat
     1681 gatgccatcc gaacggtggt ttaaccctga tggttgaaan nnnnnnnnnc cctgctggca
     1741 aatccagcca attcccagtt cacgggcgat tagggcactg tgggatagcg gacttccggc
     1801 gctaaggcag atacctttta caaccgctgg atccagttgc agtactgtag agggataaat
     1861 gttctccgcc agtagaatag ttggcgagtt aaactgcggg agttcttctt tcgtttgggt
     1921 caggtggacc agggtgcgat gcagaagatc gtccacatcg atatagcgag cctgtagata
     1981 ttcatcatcc agctgttggt attgctggct aagttcttta agaacttgct gccaggcata
     2041 ttccgccgtg caatgttcat gctgaaggag ttcgcttgcc gccgcctgca gttccggatc
     2101 atctaacagt gtatggtgac cagaaaagat tgcggcaata tcgtcaagcc cgctggtttc
     2161 tgctttcgct gttaacgtca tcagatctaa taacgtgaag tcaatggcct ggcgtaatcg
     2221 ttcttgttct tcttccacgg tcagggttga ttttgcctgt accgtacata aaacaggttg
     2281 ataatnnnnn nnnnnaaagg ctttacccga aacaggcgga acgggacgca gagtaggtgg
     2341 agcaacttcc tccgtttcac caaagttatc ttcagccagc tgacggaagg cgatcagtgc
     2401 ctcttcagct tctggtcctg tcgcaatcag gcgcagcgta tcgttatagc gaacttgtag
     2461 taacgcaatc tggttaatac tctccggtgt aacgcatttg ccgtttttct ccagcaacat
     2521 atcggcatta aatgtcgata aggtataaac cagccgggag gccggacgta catgcaggcc
     2581 gttacggttt tttatgacca ccgacagaga acgggcttct tcatcgtacg gaggaca
//
LOCUS       DAWDRC010000566         2633 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14598_length_2633_cov_2.586501, whole genome shotgun sequence.
ACCESSION   DAWDRC010000566 DAWDRC010000000
VERSION     DAWDRC010000566.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14598_length_2633_cov_2.586501"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    232..241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          633 a          673 c          646 g          671 t
ORIGIN      
        1 gaagtttcga aacggatatc cagtttctta tcgccagcaa tcggtgctaa acgccagttg
       61 ttatctgccg ccgggtgaat ttcccccgca cgtttcgact catcagcaat ccacgctgcc
      121 agcaccgagc ggttctcatc cggtgaagca aaagcgatat ggctgtcgcc cgtaccggca
      181 aatttgccgc cgtaagcgcg atagttattg gtggcaacgg ggaacatggc gnnnnnnnnn
      241 ntatcaatcg gcttgccatt aaaggtcagg ttcttaatcc tttccgcatt ggcattaatc
      301 atctggcact cgccgtcata acgggcgggc tgggtaacat caatctgata attcacacca
      361 tcaataacat caaagttata ggtgcggaaa ccatcccagt tgatgagtga ctgtggtttc
      421 gtgctgttag gatcaatctg gttaaactgt ccggcggagc actccagcca ctctttcacc
      481 tctttaccgc tggctttcac cacaatcagc gtattggggt agagataaag atcggcggca
      541 ttacggaagg tcaactggcc tttttccacc tccacatagc ttgccgggtc atttttgcga
      601 ccaccaactt taaacggtgc ggcagctgaa agcactggca gttttgccag atccggatcg
      661 ccctgaatgt aatgctcgac atacgctttt tgcgcgttgt tcaccacctg cacggtcgga
      721 tcgtcctgca caagcgccag atagctatac atattgtcgg cagatttacc gattggcttg
      781 ctgacgaact ggcgtgtggc atcgtgatcg gctttgagtg tttctaccag cttgctgtct
      841 tccgccgcga gggatttttt attcgcgatg tcgtaaatcg gccgtgcttc cgctttcgcc
      901 tgcgtcacct gccatttacc gctgtcatta ctgagttgta agtcgaccac accaagatga
      961 tcgccccaca tgcctggcat taccgccgga acaccattca gcgtgccttt cgcgatatca
     1021 gccccttcga tatcagcaaa atctttacct gggaaaacgg cgtgagcatg gccaaacata
     1081 atggcgttaa cgcccggaat ttcactgagg taataaactg agttttccgc catcacttta
     1141 tacggattgg cagacagccc ggaatgcgcc agaacgacaa cgacatcggc acctttctcg
     1201 cgcatttcag gcacgtattt gcgcacggtt tcggtaatat cattcaccgt cactttcccg
     1261 gataaattag ctttatccca gcccatgatt tgtggcggga cgacgccaat ataaccaatc
     1321 ttcagcgtct gtttttttcc gtctttatcg accacttcgg tgtctttaat taaatacggt
     1381 gtaaacattg gctgtttggt tctggcgtca atgacgttgg catttacata agggaatttc
     1441 gctcccgcca gcgcattttt cagataatcc agaccgtagt taaactcgtg gttgccaagc
     1501 gtgccgacgg tatagtccag cgtatttaat gccttataga ccgggtgaat atcacctgct
     1561 tttaatcctt tcgccgacat gtaatctgcc agcggactcc cctgaatcaa atcgccgtta
     1621 tcgaccagta cgctgttttt cacttcattg cgggcatcgt taatcaggct tgccgtacgt
     1681 accagtccga atttttccgt ggcggtgtct ttgtaataat cgaaatccat catgttgcta
     1741 tgcagatcag tggtttccat gatacgcaga tcgaccgtcg ctgcattcac actggcggca
     1801 atcagcgtgg ccaggagcgt tgcgctaaac ttaatcatca gggacatcct tttatcatcg
     1861 ggaatacgaa agaaaaggga gaataaacgt cttacttata gaacagtgaa gaatgccaca
     1921 attttacgct ttgaaaatga tgacactatc acagttggcg cattcattaa cgatagggta
     1981 taagtaaaac aataagttaa caccgctcac agagacgagg tggagaaatg ttagatcaag
     2041 tatgccagct tgcacggaat gcaggcgatg ccattatgca ggtctacgac gggacgaaac
     2101 cgatggacgt cgtcagcaaa gcggacaatt ctccggtaac ggcagcggat attgccgcac
     2161 acaccgttat catggacggt ttacgtacgc tgacaccgga tattccggtc ctttctgaag
     2221 aagatcctcc cggttgggaa gtccgtcagc actggcagcg ttactggctg gtagacccgc
     2281 tggatggtac taaagagttt attaaacgta atggcgaatt caccgttaac attgcgctca
     2341 tcgaccacgg caaaccgatt ttaggtgtgg tgtatgcgcc ggtaatgaac gtaatgtaca
     2401 gcgcggcaga aggcaaagcc tggaaagaag agtgcggtgt gcgcaagcag attcaggtcc
     2461 gcgatgcgcg cccgccgctg gtggtgatca gccgttccca tgcagatgcg gagctgaaag
     2521 agtatctgca acagcttggc gaacatcaga ccacgtccat cggctcttcg ctgaaattct
     2581 gcctggtggc ggaaggacag gcgcagctgt acccgcgttt cgggccaacg aat
//
LOCUS       DAWDRC010000567         2628 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14624_length_2628_cov_2.101438, whole genome shotgun sequence.
ACCESSION   DAWDRC010000567 DAWDRC010000000
VERSION     DAWDRC010000567.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14624_length_2628_cov_2.101438"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          619 a          647 c          748 g          614 t
ORIGIN      
        1 ataaacatcg cgatcgcttt gatatcaaag ccgatgaagg cggaattacc gatatcgaat
       61 ttattactca atatctggtg ttgcgctacg ctcacgaaaa accgaagtta acgcgctggt
      121 cagacaacgt gcgcattctg gaactactgg cgcaaaacga cattatggaa gagcaggaag
      181 cgatggcgct gacccgtgct tacacgacgc tgcgcgatga acttcatcat ctggcattac
      241 aggaattgcc gggccatgtg tcggaggatt gcttcaccgc agagcgtgaa ctggtgcggg
      301 caagctggca gaagtggctg gtggaagaat gaagtatggt attatcgcgc gcaaattttg
      361 aatctctcag gagacaggaa tgaaagtaac gctgccagag tttgaacgtg caggagtgat
      421 ggtggttggt gatgtgatgc tggatcgtta ctggtacggt cccaccagtc gtatctcgcc
      481 ggaagcgccg gtgcccgtgg ttaaagtgaa taccatcgaa gaacgtccgg gcggcgcggc
      541 taacgtggcg atgaatatcg cttctctcgg tgctaatgca cgcctggtcg ggttgacggg
      601 cattgacgat gcggcgcgcg cgctcagtaa atctctggcc gacgtcaacg tcaaatgcga
      661 ctttgtttct gtaccgacgc atccgaccat caccaaatta cgggtacttt cccgcaacca
      721 acaactgatc cgtctggatt tcgaagaagg tttcgaaggg gttgatccgc agccgctgca
      781 cgagcggatt aatcaggcac tgagttcgat tggcgcgctg gtgctttctg actacgccaa
      841 aggtgcgctg gcaagcgtac agcagatgat ccaactggcg cgtaaagcgg gtgttccggt
      901 gctgattgat ccaaaaggta ccgattttga gcgctaccgc ggcgctacgc tgttaacgcc
      961 gaatctctcg gaatttgaag ctgttgtcgg taaatgtaag accgaagaag agattgttga
     1021 gcgcggcatg aaactgattg ccgattacga actctcggct ctgttagtga cccgttccga
     1081 acagggtatg tcgctgctgc aaccgggtaa agcgccgctg catatgccaa cccaggcgca
     1141 ggaagtgtat gacgttaccg gtgcgggcga cacggtgatt ggcgtcctgg cggcaacgct
     1201 ggcagcgggt aattcgctgg aagaagcctg cttctttgcc aatgcggcgg ctggtgtggt
     1261 ggtcggcaaa ctggggacat ccacggtttc gccgatcgag ctggaaaatg cagtacgtgg
     1321 acgtgcagat accggctttg gcgtgatgac cgaagaggaa ctgaagcttg ctgtagcggc
     1381 agcgcgtaaa cgcggtgaaa aagtggtgat gaccaatggc gtctttgaca tcctgcacgc
     1441 cgggcacgtc tcttatctgg caaatgcccg taaactgggt gaccgtttga ttgtcgctgt
     1501 gaacagcgat gcctccacca aacggctgaa aggggattcc cgccccgtta acccgctcga
     1561 acagcgtatg attgtgctgg gcgcactgga agcggtcgac tgggtggtgt cgtttgaaga
     1621 ggacacgccg cagcgcttga tcgccgggat cttgccagat ctgctggtga aaggcggcga
     1681 ctataaacca gaagagattg ccgggagtaa agaagtctgg gccaacggtg gcgaagtgct
     1741 ggtgctcaac tttgaagacg gttgctcgac gaccaacatc atcaagaaga tccaactgga
     1801 taaaaaaggc taactggaaa gcggttcaca gatctcgctt cgtagcaggc aggtctattg
     1861 acgctgttgt tttttcaacc taaagtaaag gaacaagggt aagggaggat ttctcccccc
     1921 tctgatgagt tgttagtaag tcgggaaact taacagtaac aacacaacca gtatgatgac
     1981 gaacttcatc ataacccttt ccttctataa ggccccttcc tcgggagggg ctttcccgtt
     2041 tcagcccctt gctgactccc cgattgttga tgagcgccgg accccgcgca acgctttccc
     2101 agaggcagtt ttaccttccc cgagcagtca cgcaaaatgc tgcaacagcc tgatcatttg
     2161 caccttttag cggaacgcag atataaaaaa tccccggctt tttgggccag ggattattca
     2221 tagaagaggg tttactctgc tttttcttcg acgggagttg ttgagcttaa ggatgaagtc
     2281 aatgccgcca gtgagccgcc tgaaacgcca atttcattga gcaaggagtc aatcagcggt
     2341 gcctgtgtgc ggtaagagag tgcggctgat aacgcttgtt ctgccaggtt tccgcccccc
     2401 acattacccg tgtttgcatc acccgtagcg ccgccacgat tcagaccatc gacctgaata
     2461 attttgatgc cgtcgataga cttcattggt tccacggatt tctcaattac cgcaggcagc
     2521 gcctgcaata atgccagttt gaatttaagg ctggtttgtt catcagaaag tacgttgata
     2581 gcatcgttca gcgcacgttg tgcttctgct tccgccagac ccttttta
//
LOCUS       DAWDRC010000568         2616 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14697_length_2616_cov_2.474815, whole genome shotgun sequence.
ACCESSION   DAWDRC010000568 DAWDRC010000000
VERSION     DAWDRC010000568.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14697_length_2616_cov_2.474815"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          676 a          661 c          651 g          628 t
ORIGIN      
        1 ttttcgccca gggcaataaa aagataacct ttaccgtcga ataccatccg cccgccaaaa
       61 tggttgccgg tagacagttt tggcatctgg cgaaaaacgg tgcggaagtc ggtcactttt
      121 gagagatcat cacttaagcg gccataaccc acagcagttc cagctttgcc atcatcgcca
      181 acttcggaat aacttaacca gatgcggcgc gactgagcaa aatcaggcgc taaaaccacg
      241 tccagcagac cgccctgccc gtgcgcccaa acgtccggca ctccggaaag cggcgcagat
      301 aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat taacataccg
      361 tgattatcgg gtaaaaaggc cagcgcccag ggatggtcga gtttgtcttg cagtacttcg
      421 acatttaccg ttgcaggagc tgcccagaga gcggaagaaa gacaaataag gggcacaagg
      481 aaaaaggatt gtcgatgcat aacgcctccg gattgactct ggcttaaagc gtagtcagtg
      541 gaggagataa caaattcatt tttacaaaaa cttaaacatg aagggggaga cgctttctcc
      601 cccttagttt tcaggccttc tcaagcatgg cgtgcttctg caggctctgg atactcagcg
      661 ttaagctcat cagacaattt tcaagcttat cggcgttgac ggtaataaca gtcgggcaat
      721 catggtgccc actcatcaaa catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa
      781 gagcactcca ctcttcctga tccagatgaa gattcaaccg cagcgattta tcgtgcagtt
      841 cgcgattcag tttaaaaaag ttatctcgta gatgattgct ttcgctgacg gacatgtatc
      901 cttttgcctt cctcagttgc acgtatgcgg cgaggtcaac gcgtgcgtcg atcaaccggt
      961 tcagcagatc gattcgctgt ctgtcaacga acatacttcg atcctcctct ttccagcctt
     1021 gttgcaatta aatagtatgg ctgttttttg agcaaaatgc tgtccaggat caattcttca
     1081 cgccgaatta acaaaattta catttccttc aactccgcca gcgaatgaga ttgcgataca
     1141 aattttatcc agaactctgt agaatccctg ccctgacctt tcctgtaact gaatattttt
     1201 taagctatga gcaaagtaac tccccagccg aaaatcggct ttgtttccct tggctgtccg
     1261 aaaaaccttg tcgattcaga gcgtattctc accgaactac gcactgaagg ttatgacgtg
     1321 gtaccgagct atgatgatgc ggacatggtg atcgtcaaca cctgcggctt tattgacagc
     1381 gcggtacaag aatcactgga agccattggt gaagcgttga atgaaaacgg caaggtaatt
     1441 gtgaccggtt gtctgggggc aaaagaagat cagatccgcg aagtccaccc gaaagtgctg
     1501 gaaatcactg ggcctcatag ctatgagcag gttctggagc acgttcatca ctacgtacca
     1561 aaaccgaaac acaacccatt tctgagcctg gtgccagaac aaggtgtgaa actgacgccg
     1621 cgtcattatg cctatctgaa aatttctgaa ggctgtaatc accgctgcac cttctgcatt
     1681 attccgtcta tgcgcggcga tctggtgagc cgtccgattg gcgaagtgtt aagtgaagcg
     1741 aaacgtctgg ttgatgcggg cgttaaagaa attctggtga tctcgcagga tacttccgcg
     1801 tatggcgtcg atgttaaaca tcgtactggc ttccacaacg gcgagccggt aaaaaccagc
     1861 atggtcagcc tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct gcactacgtt
     1921 tacccttatc cgcatgtgga cgacgtcatc ccactgatgg cagagggcaa aatcctgccg
     1981 tatctggaca ttccgttgca gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg
     2041 ggttctgtag atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg
     2101 accctacgct caacctttat tgttggcttc cctggcgaga cggaagaaga tttccagatg
     2161 ctactcgact tcctgaaaga agcacgtctg gatcgcgttg gctgctttaa atacagcccg
     2221 gttgaaggtg cagacgccaa tgccctgcct gaccaggttc cggaagaagt gaaagaagaa
     2281 cgctggaacc gtttcatgca gttgcagcag caaatttccg ccgagcgcct gcaagagaaa
     2341 gtgggccgtg aaattctggt gattatcgac gaagtggacg aagaaggcgc gattggtcgc
     2401 agcatggcag atgcaccgga aatcgacggc gcggtttatc tcaatggtga aaccaacgtt
     2461 aagccaggtg atatcctgcg agtgaaagtc gagcacgccg atgagtacga tttgtggggt
     2521 agccgggttt aattaattca ggccgggcaa aatcatttgt ccggcctgcg aataatgatt
     2581 actgccagct aatctgcgcc ctgccgtttt tcttac
//
LOCUS       DAWDRC010000569         2614 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14717_length_2614_cov_1.826104, whole genome shotgun sequence.
ACCESSION   DAWDRC010000569 DAWDRC010000000
VERSION     DAWDRC010000569.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14717_length_2614_cov_1.826104"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          533 a          720 c          694 g          667 t
ORIGIN      
        1 gcgtcagttt ttgcagcatc tgatcggtgg tggacaccgc tttactgttg atttcgtaag
       61 cgcgttgcac ctgaatcata ttgaccagtt cttccgccac gttgacgtta gacgtttcaa
      121 catacccttg atacagcagt cccgcgccgt tcaggcccgg cgtgctttcg ttcggtgcac
      181 cggaggattg cgtttcggtg tagaggtttt cgccaatgct ctccagtccg gtgtcattca
      241 taaaggtggt gagattgagc tgcccaacct gaaccggagc tgccaggcct tgttgggtta
      301 cgctgaccac gccatcacga ccgatggtga tacttaacgc attcgccgga atggtgatcg
      361 ctggctgcac ctgaaaacca ccggccgtca ccagctgccc gttctgatcc acctggaaag
      421 agccgtcacg ggtataggct gatgaaccat ctggcaacat cacctggaaa aagccctgcc
      481 ctttaatcgc gacatctttg ctgttgttgg tctgcgacag gttgccctgg ctgtgtaagc
      541 gttcagtggc gaccgggcgt acccccgtgc cgatttgtaa tccggagggt aaggtggttt
      601 gttcggaaga ctgtgccccc ggctggcgaa tggtttgata aagcagatct tcaaacaccg
      661 cgcgctgacg cttaaaaccg ttagtactga cgtttgccag gttgttggca atgacgtcca
      721 tattggtttg ctgggcgtca aggcccgttt tggcgatcca taatgaactg atcataaaat
      781 gtcctgtagt ggattagtga caaagtgcat atgtcttctt gccggatgcg gcgtaaacgc
      841 cttatccggc ctacaagtgc tcgcaaaatc gataaattgc gattgccccc atagacccga
      901 taaacttgcg catcgggcaa cttatacttg tcatgtatcc tttcaattaa ctcatcgaca
      961 gcagttggtt ggcacggcct gcgttatcat cgacgctgct gatcaccttc atctgcattt
     1021 caaaacgccg cgcgctggca atcatgtcgc tcattgccgc aacggcattg acgttactgc
     1081 cttccagaac acccgacatc acacgcaagg ttggatctgc ctgcagtacc ggcccacgcg
     1141 tagcctggct ttctgcgctt aaacgaaaaa tgccatcgtc accgcgctgg acttcgctgc
     1201 ccgtggcttt caccagcttc agacgcccca ctggcgcaac cgtatttgcc ggatcgcccg
     1261 gattcagcgc cgagattgtg ccatcggcag caatagtgat ttccgcccct tccggcacag
     1321 caattggccc cgcctcgcct accaccggat gtccctgaat tgtcagttgc ccggtgggat
     1381 caacctgaat gctgccatta cgcgtatacc cttcactacc gtcagcggtc tgcacgacca
     1441 gccaaccatc ctgctgcaac gcaacgtcca gcgggcgcga ggtgtaatcc atcttgccgg
     1501 gcgtcatgtc tgcacccggt gttgacgccg ttaccagcgt gcgcgtgggc agtgaaagcc
     1561 cttccaccgg cactgcgcgc aaggcgttta actgcgcgcg aaaaccgggc gttgaggcat
     1621 tggccagatt actggcggtt accgcctgtt gattcagcgt ctggctggct gctcccatcg
     1681 cggtataaat tgcgtgatcc attgagctat cccgtcagcg attagcgtaa gttaaccagc
     1741 gtattgagga tctggtcctg ggttttgatg gtctgggcgt tagactgata gttacgctgg
     1801 gcaacgatca tatgaccagt tctttactga gatcgacgtt ggacgcttcc agcgcaccgt
     1861 tggtcagtgt gccaaagttg cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt
     1921 gcgtagcgga ccagacgttg tcgccttcgg atgccagacc ttcgttattg gcaaagttcg
     1981 ccagtacaat ctgccccagc agttgggttt gttcgttgga atagttgccg acaaccgtac
     2041 cgtcatcatt gatttgataa ctcaccagat cgcccggttt gtagccgttc tgggtggttg
     2101 ccacaatgtt gttagcgccg gtattttgct gcatggagtt gaggaagctc aggctaaacg
     2161 tggcgggatc tgcgccgtta attgcgccag tggaaatatt cgctgttgga tttgaggtca
     2221 gaacgccatt ggcattaaac accagtgttg tcgctgtctt cgcaatgctg tttggatcac
     2281 tactatcctg ggtgtagacc tgccagttat tatccccggt cttcacaaag tacacgctca
     2341 tgtcatgagc attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat
     2401 ccgcattgct ggcatcaaat gcgttaacag agggaagcgg atcactggaa ttcaggttga
     2461 tctgcatcga cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatat
     2521 tggtcggatt cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca
     2581 gctgtaaacc ttgcatattc accaggttac ggtt
//
LOCUS       DAWDRC010000570         2611 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14735_length_2611_cov_2.339593, whole genome shotgun sequence.
ACCESSION   DAWDRC010000570 DAWDRC010000000
VERSION     DAWDRC010000570.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14735_length_2611_cov_2.339593"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          672 a          640 c          675 g          624 t
ORIGIN      
        1 tgcgcagcgt gttcactgcc gtacaggcag cttagaaatc agccggaggc tctcaatttc
       61 agccgcgcgg gttcactgcc gtacaggcag cttagaaaag cacggctgcg gggaatggct
      121 caatctctgc gttcactgcc gtacaggcag cttagaaatg atggcgcagc agtcctccct
      181 cctgccgcca gttcactgcc gtacaggcag cttagaaact gaacgttgaa gagtgcgacc
      241 gtctctcctt gttcactgcc gtacaggcag tattcacact acgatagtgt tagacgtttg
      301 gtcgtgcaat gacactctca acttcaaacc attagcgtta gcacgcaata acaatcgtaa
      361 taattgcgat ggaaatcaat tttcagcaca taaatcaatg ctgtactaag cccaatacct
      421 tcaaatataa aataatcaca ggatgtgttt atgtcttcga attaccttac gccttccgat
      481 ctcaaaacca ttctccactc caaacgcgcc aatatttatt atctggaaaa atgccgggta
      541 caggtgaatg gtgggcgggt ggagtatgtt accagcgaag gtaaagagtc gtactactgg
      601 aatatcccca ttgcgaatac cacggcgttg atgctgggaa tgggaacttc cgttacccag
      661 gcggcgatgc gtgaatttgc tcatgccggg gtgatggtag gcttttgtgg tacggatggc
      721 acgccgctgt attcagcaaa tgaagtggat gttgatgtct cctggctcag cccacaaagt
      781 gaatatcgcc ctacagagta tctccagcag tgggtttctt tctggtttgt tgaagacaaa
      841 cgacttgctg ccgcgaaacg ttttcagtta atccgcctga cacatatcga taagcactgg
      901 tccagctcga aaatgctgcg tgaacacgca tttcaacctg atgttaatgc gctgcatacc
      961 ctcctgaatc gtacttgtga agaaatagac gcggcagaaa accatacgca gcttatgctc
     1021 gttgaggcca aattgactaa agcgttatac aaaatggtca gtcaaaccgt ggggtatggc
     1081 gatttcactc gcgcaaaacg gggcggtggt atcgatatgg ccaaccgctt ccacgatcaa
     1141 gggaattatc tggcttatgg actggcagca gtggcggcgt gggtaacagg tatcccgcac
     1201 ggtttagctg taatgcatgg taagacacgt cgcggcgggc ttgtttttga ccttgccgat
     1261 ctgattaaag atgctcttgt tatgccgcaa gcatttattg ccgcaatggc gggagaagat
     1321 gctcaggagt ttcgtcaacg ctgtgttaat atctttcaac aagcggatgc gctggatgtc
     1381 atgatcacct ctctgcagga aacggcccag gcactggcta aggctgacca atgaatgtcc
     1441 taattatctc acgctgtacg aaaaatgctc gcgtggaaag ctgccgtatt attgatcagt
     1501 ttgccgaacg cactggtgac gcagcatggc aaacggtgat tactctggaa ggcgttaata
     1561 cactccgccg tttactgcgc aaaaccgcca ggcgaaatac cgcagtggct tgtcactggc
     1621 tgaagaataa tgggcaaacg gagctgatgt ggattgtcgg caatattcgt cgctttaatg
     1681 ctcaaggccg cgttcccaca aatcgaacaa cgcaaatcag cctggtgaac aaagaagaaa
     1741 atcgctggca gtgcgcggaa agtattgcac tgctggcggc tatcgctggg ctatttcatg
     1801 attttggtaa atcaggacgc agctttcagc aaagcctgac tcaaaagaat acccgcagtt
     1861 atcaacctta ccgacatgaa tggatctcac tgcggctctt tcagacgttt gtcggtgatc
     1921 ggcccgacga tgtgtggctg gcgaagcttg gcgaactcac ggcagatgat gaatctgcga
     1981 ttcttgagcg cctgcaaaaa gacactcctc aattttcaaa tagcccgttc agttcgctgc
     2041 aaccattagc caaaacggta ggctggctga tcctttccca tcatcgaatg ccggagaatc
     2101 tttctccaca gtcggaacaa tcatgtaaag ggtgtgaaag ttggctggag aagcaactta
     2161 atgctgactg gaatgccgtt aaccaccgca gagatgactg gtcacaacag gattttgcgc
     2221 acgtctggca gtttccatca ggaacgccgc ttcaaagcgc gagctggcgg gaaaaagcgc
     2281 gtcagatagc ccgccgggca agcaacgcgc tcggtttaaa gacatatggg cagatggaac
     2341 agttgttccc cttacatatg gcgcgttttt gtctgatgct tgccgatcat tactattcct
     2401 caagcgatgc gcagcaaaaa tggcaggacc cggattatat cgtctgggca aatagcgatc
     2461 gccaaagcgg cacgctcaaa caacgggtag acgaacacct tgtgggcgtc gcgcatcatg
     2521 cgtatctgct tgggcggcaa ctgatgcaca cacgggcatc gcttccggca attgcccgac
     2581 gtaaaggttt tagccagcgg gctacagatg t
//
LOCUS       DAWDRC010000571         2605 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14776_length_2605_cov_2.528627, whole genome shotgun sequence.
ACCESSION   DAWDRC010000571 DAWDRC010000000
VERSION     DAWDRC010000571.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14776_length_2605_cov_2.528627"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          686 a          676 c          602 g          641 t
ORIGIN      
        1 tcatccatcc gcttaccgga gaacagcgca gcatctgttt gccgccccat cggattaatg
       61 aatttttccg ccaaccgttt acgacgcgct agcgcgccgc gaagaatacg ggcacggcgt
      121 gattcatgct gtttgccgtg attttcatct tcatactctt cgtcgtcttc atactcatct
      181 tcatcgaccc aggtatcatc gcgacgggta cgattactgg cgaaggtaag aatatttaaa
      241 atccagccgc cgagtttttc agcaatggtc acccatgacc aaccggtgaa cagcgtcagg
      301 cccgctgccc aaacgcagag cagcgcaata gttcccccgc tactgtgtag cagtggttgt
      361 agcgtagtgc ttagtaagct gccaatgacg ccaccggagg caaaatacca gatatcgtca
      421 gcgttgattg ccgccagacc acaggaggta aggatgagcg ccaaaacgcc aatgatgcgt
      481 agcgaaacgg caaaataatc aatgtattcg tcgctggact gatgacgcca ggcaaaccaa
      541 caaccgccga caataatgac gggaatggtg taagccatca cgccaaaaat aaagaacagc
      601 gtatctgcca gccacgcacc gggcatccca cctaaattat ggataggttc atgccaggcc
      661 gtttgcgacc agctggggtc cgaagggtta aagcttagta aggcagccat caaccagacg
      721 gcaaacagga caataaggat cagcaacgcc tccagaagac ggcggccgct acttaacttt
      781 gtcaatgtga cttctttgtc ttcagtgtat tcctggctca agaaaggctc tccaggttac
      841 aggcttttcc tgcccgatgc taaaacggac aacagtacca ggttacccca gtactgttgc
      901 tgtatggatt aacaggagtg taatcaaaat acgccgattt tgcacctgtt ccgtgttagc
      961 gcgtcttaat aaccagacga ttactctgct tgacttcttc cataacaacg tatgtccgtg
     1021 tgtcattgac gccaggcaga cgcagcaggg tttcccccag taacttacgg taagcagaca
     1081 tatccggcac gcgtgttttc aacaggtagt cgaaatcacc ggatactaaa tgacactcct
     1141 gaatttcttc aagtttttgt acagcggtat tgaattgttc aaacacatcc ggtgcgccac
     1201 gattcagagt aatctcaacg aataccagaa gtgatgcatc cagataatgg gggttcagca
     1261 gcgccgtata gccctgaata aacccttgtc tttccagccg acgcacacgc tcaaggcacg
     1321 gcgttgggga aagtcccaca cgtttagaaa gctcgacgtt agaaatacgc ccatcctttt
     1381 gcaactcatt aagaatgtta cgatcgatac ggtcgagatc tttgccaggg cgcttcttgc
     1441 tatctaccat tattattgtc tctctgtatt ccttccctac tcctgtctga ccctgaaaca
     1501 tcactgttca gagtcgctac ccgtcacgat agaccgaaat catgaagatt cacggcgcgc
     1561 aatgcctggg tctacggtga gaagaccgtt gccttgtggc agctatcgac atcacgaatg
     1621 gcgtctgtcc acgccatacg tagatttcac tagcacggta aacatggtat ttacatgcat
     1681 ggttatgcgc atataagaat actgtttttt ttcttcctcg aatgttttcg caaaacacca
     1741 ggggattgtc aaagcaaaac atcgattttt agtacaacat gccagatatt cattaacagg
     1801 ggcgtttatt catcatttaa tcggcttata aagcgggaaa tttaagtagc agaggtgata
     1861 atgacgtgag aatttgacct gcttatcgat gctgccgatt gcacaaattg ttaacaaaat
     1921 cgtcatactc tttttttacg tctgtaaatt ccctacaatc ctgccattgt ctgccaacaa
     1981 ctatggggat ctcatgggca cgaccaaaca cagtaaactg cttatcctgg gttcaggccc
     2041 ggcgggatac accgctgctg tctacgcggc gcgcgccaac ctgcaacctg tgctgattac
     2101 cggcatggaa aaaggcggcc aactgaccac caccacggaa gtggaaaact ggcctggcga
     2161 tccaaacgat ctgaccggtc cgttattaat ggagcgcatg cacgaacatg ccaccaaatt
     2221 tgaaactgaa atcatctttg atcacatcaa caaagttgat ttgcagaatc gtccgttccg
     2281 tctgactggc gatagcggcg aatacacttg cgacgcgctg attattgcca ccggagcttc
     2341 tgctcgctat ctcggcctgc cctctgaaga agcctttaaa ggccgtggag tttctgcttg
     2401 tgcaacctgc gacggtttct tctatcgcaa ccagaaagtt gcggtcatcg gcggcggcaa
     2461 taccgcggtt gaagaggcgc tgtatctgtc taacatcgct tcggaagttc atctgattca
     2521 ccgccgagat ggtttccgtg cggaaaaaat cctcattaag cgtctgatgg ataaagtgga
     2581 gaacggcaac attattctgc acatc
//
LOCUS       DAWDRC010000572         2604 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14788_length_2604_cov_2.234994, whole genome shotgun sequence.
ACCESSION   DAWDRC010000572 DAWDRC010000000
VERSION     DAWDRC010000572.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14788_length_2604_cov_2.234994"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1258..1267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          631 a          592 c          669 g          702 t
ORIGIN      
        1 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
       61 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcca tgtttaagta
      121 agtggtttat cccctttttg ccgcggttat aaaggggata gccaacaatg accccacgac
      181 tcatctcaat tatctcgccc cgacctggct aagtcccagt ccgatctctt gcagtcgcgt
      241 tcacacctga atgacttctc ctgaacttaa gtctcaggcc tatttggccg ggaatccctc
      301 tcgaatttgc tctttttgcg tcccgccttc catcaacctg taacagcccc agctattttc
      361 tgcgtaattg tgatctaagt tgtaaaacgc agatcattat ctgtgtttta cagatttgat
      421 gttgacctca aaaatcatgt gggtaagtat gatgaccatc taagcaaaac aaaacacaga
      481 ggatgacgca atgaaagcac tggctcgatt tgtggcccgg aagatgtcgt gattgaaatt
      541 aaagccgcgg caatctgcgg cgcagacatg aagcactaca atgtcgatag cggttctgat
      601 gagtttaact ctatccgcgg ccatgagttc gcaggttgta ttgcgcaggt tggtgaaaaa
      661 gtcaaagact ggaaagtggg gcaacgcgtc gtgtcggata acagcggtca cgtttgcggt
      721 gtttgcccgg cctgtgaaca aggtgatttt ctgtgttgta cagaaaaagt aaaccttggt
      781 ctggataaca atacctgggg cggtggtttt tccaaatatt gtcttgttcc tggtgaaatt
      841 ctcaaaattc atcgtcatgc gttgtgggaa atccctgatg gtgttgatta tgaggacgca
      901 gccgtacttg accctatctg taatgcctac aaatccatcg cgcagcaatc gaaattcctc
      961 cctggtcagg atgtggtcgt catcggcact ggcccactcg ggctgttctc cgtacaaatg
     1021 gcgcggatta tgggggcggt aaatatcgtc gtcgttggtc tacaagaaga tgtggcggtc
     1081 cgcttcccgg ttgcaaaaga actgggtgcg acggcagtag taaatggttc taccgaagat
     1141 gtggtggcgc gctgccagca aatttgtggc aaagacaatc tggggctggt gattgaatgc
     1201 tccggtgcca atatcgcatt gaaacaagcc atcgaaatgc tccgcccgaa tggggaannn
     1261 nnnnnnngaa gtagtacgcg ttggaatggg cttcaaacct cttgatttct cgattaatga
     1321 cattaccgcc tggaacaaaa gcatcattgg gcatatggcc tatgactcca cctcatggcg
     1381 taacgctctc cggctattag ccagcggcgc tatcaaagtc aaaccgatga tcacgcatcg
     1441 tatcggcctg tcgcaatggc gcgaagggtt tgatgcgatg gtcgataaaa ctgcaatcaa
     1501 agtgatcatg acttacgact ttgatgaata actactctcc tgaaggaacg ttatcgcatg
     1561 gaacagataa caaaaccgca ttgtggtgca cggctggatc gcttaccgga ttgccgctgg
     1621 cattcgtcaa tgtttgccat cgtcgcgttt ggcttgcttg tctgctggag taatgccgtt
     1681 ggtggcttga tcctcgcgca gctgaaagcg ttgggctgga cagataattc caccactgcc
     1741 acattctcag caatcacgac cgccggaatg tttctcggtg cgctggtggg cggcatcatc
     1801 ggtgacaaaa ccggtcgcag aaatgcgttt atcctctatg aggccattca tattgcttcg
     1861 atggttgtcg gtgctttctc accgaatatg gatttcctta ttgcctgccg ttttgtgatg
     1921 ggcgttgggc tgggagcttt actggttaca ctgtttgctg gtttcaccga atatatgccc
     1981 ggaagaaatc gcggaacgtg gtccagtcgg gtttccttta ttggtaactg gtcatatccg
     2041 ctctgttcat tgattgcgat gggactcacg ccgctgatta gtgcagagtg gaactggcgg
     2101 gtacaactgc ttatccctgc aatattgtca cttatcgcta cggcgctggc ctggcgctac
     2161 tttcctgaat ccccgcgctg gctggaatcg cgcggacggt atcaggaagc cgagaaagtc
     2221 atgcggagta tagaagaagg cgtcatacgc cagacgggta aacctttgcc gcctgtggtt
     2281 attgctgatg acggtaaagc accacaagcg gtgccgtatt cagccttact gacaggagta
     2341 ttactgaaac gcgtgatatt aggctcttgt gtgctgattg ccatgaacgt tgtgcagtac
     2401 acactaatta actggttgcc aacgatattc atgacccagg gaattaattt aaaagactcg
     2461 attgttttaa ataccatgag tatgtttggc gcgccatttg gtatttttat tgccatgctg
     2521 gtgatggata aaattccgcg taaaacaatg ggtgtggggc tattaatcct gattgcggtg
     2581 ctcggatata tctattcact gcaa
//
LOCUS       DAWDRC010000573         2603 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14791_length_2603_cov_2.203689, whole genome shotgun sequence.
ACCESSION   DAWDRC010000573 DAWDRC010000000
VERSION     DAWDRC010000573.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14791_length_2603_cov_2.203689"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    733..742
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          600 a          624 c          609 g          760 t
ORIGIN      
        1 tcgtgacgcc cggcaacgct attgagaatg taggactcca gcatggtatc aaacgcaatc
       61 ccacgcagtt caatgccgta gttcgccaga ataccgcgat cgtatttcag gttttgcccg
      121 accttcagcg ccttttcatc ttccagcagc ggttttagca actcgagtgc acgctcgcga
      181 gagatttgat cgggcgcatc aagataatca tgagcaaccg gaatatatgc cgctacgcct
      241 ggctcgatag caaaagaaag cccgaccagg ttagcagaga tgttatcaag gctgtctgtt
      301 tcggtatcaa atgcaaatac cggcgctttt tccagcttcg caatccactc tttcagtgtt
      361 tcttcatcaa agatggtgac gtagttgtca taagaaatca ccgttgccgt cacttctggc
      421 gcttcgtctg caacactggt ttcctgcggc ctggctgcgg gtttcgcccc tttagcctgt
      481 agccattttc ccgcttcgac atcagcagtc cagcgtttga actcatactt tttgaacaga
      541 cctaacaact cttccgccgc cggtggctgc acttccagtt gttcacaggt cagctccagt
      601 tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat aagcaacttc tttgttttgc
      661 tcgagcttgg ctgccattgt tttcgcgcca cggaagctca acccagcaat tttttctggc
      721 tcggcataca gcnnnnnnnn nnttcaccga cgcccggtac gccagggatg ttatcggagg
      781 agtcacccat cagcgccagg aaatcgatga tcagttctgg cggcacgccg tacttattca
      841 ccacctcttc cggtccgagg atggtattcg tcatggtgtt gataagcgtg atatttggtg
      901 tcaccagctg cgccatatct ttatcgccag tgctgatcag caccggacgc ccggtttttt
      961 cggcttcgcg cgccagagta ccgataacgt cgtccgcttc tacgccagaa accgccagca
     1021 gcggcagtcc catcgcttta accatcgcgt gcagaggttc gatttgtgct cgcagatcgt
     1081 ccggcattgg cgggcgatgt gatttgtaat gttcaaacag ttcatcacga aaggtttttc
     1141 ccttggcgtc aaagaccacc gctgcatgcg tcggtttata ttgcatgatc agactgcgca
     1201 gcatgttgag gacaccatac atcgcaccgg tcggctcgcc tgcgctgtta gtcagcgggg
     1261 gaaacgcgtg atatgcgcga taaagataag atgaaccatc tacaaggata agtggatttt
     1321 gggggatctg aaccataatg tccgtgcctg tttatcagat tatgggtaaa ggatgccaca
     1381 gacaggatga aaacatgagt ttttcaccgc ttttcgtgaa aatcttttgt agatcttctg
     1441 gatcgctcgc aaatcgtcat gtggataact ttgtgcataa attatttaca tcgcttttaa
     1501 tttattatca tgctttttac agcatgatgt ttatctttgt ttttcatgca gttaaaaata
     1561 aagccatccc taataattga gatgatggga ttttttctaa tgtggataga tatgaattat
     1621 ttttctcctt aaggatcatc cgttatttgg gtcgttttct gctaaaatct gcgccttgcg
     1681 cttctttagc gcactgtcta tggctaacct tttgaataaa ttcattatga cgagaatact
     1741 cgctgcgata acccttttgc tgagtatcgt attgaccatt ttggtcacta ttttttgttc
     1801 cgtcccgatc atcattgccg ggattgtaaa actattgcta cctgtgccag tcatttggcg
     1861 aaaggtttcg cgtttttgtg attttatgat gtattgctgg tgtgaaggtc tggcggtatt
     1921 actgcatctg aacccacacc tgcaatggga agttcacggg ctggaagggt taagtaagaa
     1981 gaactggtat ctgcttattt gtaaccaccg tagttgggca gatattgtcg tactgtgcgt
     2041 gctgtttcgt aagcacattc cgatgaataa atatttcctc aagcagcagc tggcctgggt
     2101 gccttttctt ggcctggcgt gctgggcgct ggatatgccg tttatgaagc gttattcccg
     2161 cgcttattta ttacgtcatc cggagcgacg tggtaaagat gttgaaacca ctcggcgttc
     2221 ttgtgaaaag tttcgcctgc accccaccac tattgtcaat ttcgttgaag gctcccgttt
     2281 cacgcaagaa aaacatcagc aaacccactc ctcttttcaa aacttgttgc caccaaaggc
     2341 ggcaggcatt gcgatggcgc taaatgtact gggtaaacaa ttcgataaac tgcttaatgt
     2401 tacgctgtgt tatccggaca ataaccgtca gccgttcttc gatatgttaa gcggtaaact
     2461 gacgcgaatt gtcgtacatg ttgatttaca gcctattgcc gatgagttac atggcgacta
     2521 catcaatgac aaaagcttta agcgccattt tcagcaatgg ctgaactcac tgtggcagga
     2581 aaaagaccgg cttctgacgt cgt
//
LOCUS       DAWDRC010000574         2602 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14799_length_2602_cov_2.122497, whole genome shotgun sequence.
ACCESSION   DAWDRC010000574 DAWDRC010000000
VERSION     DAWDRC010000574.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14799_length_2602_cov_2.122497"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          667 a          578 c          694 g          663 t
ORIGIN      
        1 tatctgaaaa tgctttgcca gagccacaaa ctggcgggcg gcagcattgt cagtatgcgc
       61 gatccgcagc tttttcatgc gcctgaagat gaaaagcaac tgcgtaaaat cgtctggcgg
      121 ttaatgccgg gttatgcgct ttacgatcgc agtgaatggc tggctgaaca tcaccaacaa
      181 catcccgaca tctcgttgct ggatgcctgg cttgatttcg ccgcgattga atctccggct
      241 gaagacaaca gcgcaaaatg ggtataccag ccgaagccaa tgcctggttt cctggtaccg
      301 ttaatgtgtg gctaccaacg catttctcct gtttacgcac ccggtgaagt tgaaaatgcg
      361 cgcgacaccg tgacgccatt tgcctttgcc gaagcggtat atggcattgg cgaatggcgc
      421 ggattacatc gcactacaga cttgcaagcg ctgatgtggc gttaccgcac aacggacact
      481 ggctactatt gttcggcaac gccagttgtt gatgatttta cctttaatga atatgacgat
      541 ttagaataag gacataatta tggcaaaggc tcctgtagca ataaaaacgg cgtcggtact
      601 ggcatttgag cgcaaactgg caacctctga cgcggtaatg tacgcaggta actggcaagg
      661 aaatgaatgg caacccattg agattcagga gaaagcggtc cgcggaacca tctctaatcg
      721 cctgaaaaat gccattgtca gcgatcccac aaaactggat gctgaaatcc aaaaagccaa
      781 cctgcaacgg gtggatgtgg cttcgcttcc ggtagatacg gatacgttaa aggtggtgtt
      841 tacgctgcgt gtgctgggta atttatccac accgtcagta tgtaatgata tggcttacca
      901 ggaggcgctt tccggggtca ttgaaggtta catcagcgaa catggcttta aagagctgac
      961 gcttcgctat gcgctgaatc tggcaaatgg tcgttttctg tggcgcaacc gcattggtgc
     1021 cgaacagatc caggtgaaag tgactgctaa cgagacgtca tggactttta acagccatga
     1081 cttctcttta cgtcaatttg accagggaca ggcagcggtc acgcagctgg cggccattat
     1141 agagcaagga ttaagcgcaa aagagtgggt gatgcttacc gttgaagcgc aggttcgtct
     1201 tggtgccggg caagaagttt ttccgtcgca ggagctggta ctcgacagca acagtagtaa
     1261 aagtcgcgtg ttataccagg tggctggcat tgccggtatt cactctcaga agattggcaa
     1321 tgctttacgt accattgata cctggcatcc aaaggttgat gaattggggg ctatagccgt
     1381 ggaaccttac ggttctgtaa ccagtcgcgg tgtggcctgc cgtcaaccga aagaaaaact
     1441 cgacttttat accttactgg acaattgggt gactaaaggg atgaaaccag acgttgagca
     1501 gcagcactac gtgatggctg tgctgatccg tggcggtgtc tttggtgaga aatcggaata
     1561 aggagctgcc aatggatcac taccttgaga tccgcgttct gccagatcct gaattttcga
     1621 gcgaaatgtt gatggcggcg ttatttgcaa aactgcatcg ggtattaggt gcaagagggc
     1681 aaggcgatat cggcgtgagt ttccctgacg taaatgttat gcctggagcg cgtttacgct
     1741 tgcatggtag cgcccaggca ttgcaggcgc ttgaagcctc aacgtggcgt aaaggactaa
     1801 cggactattg ccaatgttca cctgtcactc ccgttccaga aataaaaggc tggcgagtag
     1861 taagccgtgt gcaggttaaa agtaaccctc agcgtctgtt aaggcgttca gtgaaaaaag
     1921 gctggctaac tgaagaacag gcaattgaac gactggcgac gcaggctgaa cagcggactg
     1981 atttaccttt tctcaatatg aagagtttgt cgagtcagca actgttcaag ttgtttatcc
     2041 gtcatggtga tttgctcaaa gaaccggtta aaggggaatt tagtagtgat ggattacgcg
     2101 catccgccac tattccctgg ttttgatgct ttgcaatttt taacaggcct ggccatgtgg
     2161 ttgggccttt ttatgcgcgc catgcccacc gcatacaaaa aaagcccgta ctttcgtacg
     2221 agctcttctt taaagatggc ggtgaggcgg ggattcgaac cccggatacg ttgccgtata
     2281 cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg ttttgctgcc
     2341 agacctcata ggtggcaacg gggcgctact atagggagtt ggagtgaaac ggtcaagaag
     2401 aatttatata gattgatttg tttggttacg caatgaacac gctgttcgcg ggacggagat
     2461 tatgaccgta tgtgttctgg tcaattgttt atcaaaagct atgcagaaaa tatgagattg
     2521 aagaaatacc aaaccgaccc tttttctagg ttgtaatgta actcattgat tttcttattg
     2581 ctattttgaa gtctggaaaa ag
//
LOCUS       DAWDRC010000575         2569 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_14998_length_2569_cov_2.202068, whole genome shotgun sequence.
ACCESSION   DAWDRC010000575 DAWDRC010000000
VERSION     DAWDRC010000575.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14998_length_2569_cov_2.202068"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          641 a          660 c          656 g          612 t
ORIGIN      
        1 aatatattca accaagaaat agaataatat actctagata attcaagtcg caggaaggcg
       61 ataagcgagt gaatccccag gagcttacta aagtaagtga caggggtgaa cgacaaatcc
      121 gcatggagcg gatttgaacg ctacttgtag cggccccgta aggggtgagg tccatggatg
      181 ggccgaataa cccgcagtcg cagcacatgc aacttgaagt atgacaagta aagctttatt
      241 aaagggggaa acggtattcc cccttaaatg cgtttcaatt tgcccgttac ttcaacaaat
      301 caatcaaccg taacagagcc actaataact ccagtacggc aatgagcacg gttaactttt
      361 gtgtcagcgt gtccattcgc tctccttatt aaggagcgca acaccagcat cttaccctta
      421 cactgcctgt cgatactggt ttacgtatgt tgagtcgcca tgttgcactc cggatcagag
      481 cactacctgc ggcaccaccc gtccggtagc caggacttga aattggttcg atccggcgca
      541 acttcccaac agtcaggatt ataaatctca ttatcttaat tgcaacgggg tccgtaggtg
      601 gttataattc gttcgttggg gcgccatgtt gcataatcag taacttaacg tcaaagttgt
      661 aaataaaaga acctgcctca ccagcaggtt tttttatttt ttgcgatctg ctttccagat
      721 attttccgct caaacaacta atgcgccaaa catttattgc gcgtaaaata tcgtttattt
      781 cattaataca tttcagggat gaatatatgt caccgcagaa taatcacctg cagcgtccgc
      841 ctgctgctgt gttatacgcc gttgaactgg caaaattaaa acaaaatgat aacgcacctt
      901 gcccgccagg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt ggcgacgaag
      961 cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt atgttagtca
     1021 ctcttgccac cgggcgcggt ttgatgttgg tgggggaacc tggcaccgca aaatctctcc
     1081 tgtctgaatt actggcaacc gctattagcg gcgatgccgg gttaaccatc cagggcggcg
     1141 catctactac tgaagatcaa atcaagtacg gctggaacta cgccctgctt atcaaccatg
     1201 gtccgtcaac ggaagcactg gtccctgccc cgctttacca gggaatgcgc gacggcaaaa
     1261 tcgtccgttt tgaagagata acgcgtacac cgctggaagt acaagactgt ctgttaggta
     1321 tgctttctga cagggtgatg acggtgccgg aactcaccgg tgaagccagt cagctctatg
     1381 cgcgggaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc gtcaatgaaa
     1441 tgagtgccgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt atggatttcg
     1501 cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat agcggcattc
     1561 cgcataaagt gcctgacgcg gtactggagt tactggtccg cacctttcgc gatctgcgtg
     1621 ccaacggcga aaagaaaacg tcaatggata cactgacagc gattatgtcc accgctgaag
     1681 ccgtgaacgt ggcccacgcg gtgggcgtcc gcgcctggtt tttagcgaat cgcgcaggtg
     1741 agccggccga tctggtggac tgtattgccg gaaccatcgt caaagataac gaagaagacc
     1801 gcgcgcgcct gcgccgttac ttcgaacaac gtgttgctac acataaagaa gctcactggc
     1861 aggcttatta tcaagctcgc caccgcctgc cgtgaggaaa gatgtatgag cgagccgtta
     1921 attgtcggca tccggcatca tagtccggcc tgcgcccggc tggcgaaatc gttaatcgaa
     1981 agccagcggc cacgatacgt attgattgaa ggcccggctg attttaatga ccggatagac
     2041 gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca gtatcaggac
     2101 ggtgcggccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc ggagtggcag
     2161 gcgctacaag ccgcacgtcg tattcaggca caaacttact tcatcgattt gccttgctgg
     2221 gcgcagagtg aagaagagga cgattcgcct gatacacaag atgaaagcca gaccttactg
     2281 ctgcgtgaca cccgcatgga taacagcgat accctgtggg atcacttatt cgaagatgaa
     2341 agccagcaaa ctgcattacc ctctgcactg gcgcgctatt ttgcccaact gcggggcgat
     2401 tcccccggag atgctctcaa tcgtcagcgc gaagccttta tggctcgctg gattacatgg
     2461 gcgatgcagc aaaacaatgg cgacgtgtta gtcgtctgcg gcggctggca cgctcctgca
     2521 ctggcaaaaa tgtggcgcaa atgcccgcag gaaatgaaca agccagaat
//
LOCUS       DAWDRC010000576         2563 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15033_length_2563_cov_1.912679, whole genome shotgun sequence.
ACCESSION   DAWDRC010000576 DAWDRC010000000
VERSION     DAWDRC010000576.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15033_length_2563_cov_1.912679"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1591..1600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          557 a          615 c          673 g          708 t
ORIGIN      
        1 gtcgctttgc cggtgccgtc gcgccgcaat atctgaatat caccaccgga ggacagcact
       61 aatgcaattc ctgaatatgt tcttctttga tatctacccg tacatagccg gggcggtctt
      121 cctgattggt agctggctgc gttatgacta cgggcagtat acctggcgcg cggcgtccag
      181 ccagatgctg gatcgcaaag ggatgaatct ggcgtcgaac ctgttccata tcggtattct
      241 ggggattttt gtcggtcact tcttcggtat gctgacgccg cactggatgt atgaagcctg
      301 gctgccgatt gaagtgaagc agaaaatggc gatgtttgct ggtggtgcca gcggcgtact
      361 gtgtctgatt ggcggcgtgc tgttgttgaa acgtcgtctg ttcagcccac gcgtgcgtgc
      421 aacgactacc ggagcggata tcctgatcct gtcgctgctc gttatccagt gcgcgctggg
      481 cctgctgacc attccgttct ccgcccagca tatggacggt agcgagatga tgaaactggt
      541 tggctgggcg cagtcggtag tgaccttcca cggtggcgct tctcaacatc ttgatggcgt
      601 ggcgtttatc ttccgtctgc acctggtgtt ggggatgacg ttattcctgc tgttcccgtt
      661 ctcgcgtctg gtacacatct ggagcgtccc ggtggagtat ctgacgcgta agtatcagct
      721 ggtgcgcgct cgtcactaag cgaattttag ttcacataga ccctgcttcg gcgctgaggg
      781 atccccataa atcagcgata ataaaccaac accatattct ggtaggttct ggtgaggtgg
      841 ttaagaatgg tgcttagcac cgttcgtttt aaaattagcg gggagtgtcg taagacattc
      901 tccgctaacg tcagatacga gattacccgc cgcgttttaa tgctgttggc ctgatatcta
      961 agatgtaacc cttgattttc agcgtgatag ccaataagcc acagcactat tgtgctcagt
     1021 gttgccagca ggctcagtac cagcattctt cctgccgaac agctgtaact ggctcgcaga
     1081 ccgaacccaa aacactcact tttttcatcc ctgaagtttt gctcaatctg cattcggcgg
     1141 ctgtataact tcatgatttc tcgcggttta aagtcatctg tgctgctgaa tattaaccat
     1201 ggttcttttg ccgccgacct ggcatctttt atctgtgatg gtctggctat accacaacgc
     1261 gaacgtttat ttctccggcc tttcggctct tttttgtgca aataaaaatg accatcgcaa
     1321 cgggcgtatt ccgcccggga aagcgtcccc ggccccagat attcaggttt gttactggcc
     1381 tgtaattcct gacgcctgaa ccagcattcc ccttttctcg ccagatgcag ttgtttattg
     1441 cctcgtatcc ggccaataaa atcccagccg aatgatttta tgtgccgaaa ccaggcattc
     1501 tggaaccctg catccgtgac gatgattacc cttgcttttg ggtttaccgc ttcggaaaaa
     1561 cagcaaaatg caaagataca gcaagccttt nnnnnnnnnn agtattaaga aaggcttgct
     1621 gtatctttgc attttgctgt ttttccgaag gaactatcca actcagcaat ggaatcgaat
     1681 gtccatcgca aatcaggctg gcgcggagta cgtgatactc ctgagagggg tatccactcc
     1741 agtcaacggc gatgacacac agtgacagtt tgctcgtcag catggaaata atgtttctga
     1801 aaatcagggg gatgtcgtga tgaagagact cattaccgag cggacgatca acacgtttta
     1861 ttttattttt gacctgagca ttaccgggta aataacgtcc gatgctggtc agcgtcagag
     1921 atgcgccatt gattagtgcg atagtggcat ccaacaaagc attttgtcgg tatttgtgaa
     1981 atggtgctaa agcatcgcgg aaaaaattct gacatacttg gcgagcaggc atagaggaga
     2041 tctcattgaa ttggttgcac aagtcagtag atcacaaaac tctatgcctg tcttttttta
     2101 cccgcccatt actggggatt cctcagtaat gggcgggttt tttttatggg cacggtgcgg
     2161 ggggagttgt cggatgcgct tcgcttatcc gacctacagg ggaggatatt ttaggtccgg
     2221 taaacgcggt gccattgggc agaattagct cggagtaaca ggttttgagt atttcaggaa
     2281 gaaatggtgg tgggggaagg attactcagc gctgcgcgct tcgccctacg ggtcgttgcc
     2341 tgcggcaacg ctctctcgct ggcgctcgag tcgaaccttg gtcgaagctt ctcatccttc
     2401 cccgcatggg cagaatattc aattgcggat tcgtttgaga attcaggggc ttttgaaagc
     2461 gatggtggtg ggggaaggat tcgaaccttc gaagtcgatg acggcagatt tacagtctgc
     2521 tccctttggc cactcgggaa cccctccacg ggtaatttat att
//
LOCUS       DAWDRC010000577         2552 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15112_length_2552_cov_2.419704, whole genome shotgun sequence.
ACCESSION   DAWDRC010000577 DAWDRC010000000
VERSION     DAWDRC010000577.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15112_length_2552_cov_2.419704"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          652 a          545 c          597 g          758 t
ORIGIN      
        1 ccagcgcacg accgcgcatc atgccataga catgaatgtt accatcggca atcaattcgg
       61 cccctgcgct aacgtggctt gtaacaatca gatcacattg tggagcataa atacgctgac
      121 cggaacgcac tggggtatca attaaacgcg tttttgtgac cggcgttgta ttttgcgctg
      181 gagcctgcgg tgcgggagct ggacgcggcg ctttttcctt tccttccgtc aggataggca
      241 gccccatctt ttcaatttcg gctttaagtt gcgcatcttt gcagccactt acgccaataa
      301 cccgcaaacc agttgccgaa accgccttat gcatcgccga ccagtttacc gggtcttcca
      361 gtgcactgac gttgagtaca acgggggcat gttttaaaaa tgcgggggcc tgagcgattt
      421 tgtcttccag cgcctgatga ataaccttag gttctgcctc atgcagatga accacagata
      481 aagtgaagct actgccttta agctcgattg gcgtgtttga catcctggcc ttactcaatt
      541 agctattaat catcgccagc acgcgatgat gttccgaaga ctataaggca tgttatagtc
      601 tggattatat tgaggcaagt caccctccca tttattcaga gtaaaagtct attctgtgat
      661 aaatggcgct gattcatagc ttaaaaaata cccttgtcaa tcaacccatt gccgtcgtac
      721 ttttgattgt tcttatttac gcttcctttt tccgcaccct aatcgtgaca gcatcttcag
      781 acctgtttaa atatccagtg tttaggcgag tgtatttttt caccacgcca ggctttctta
      841 actttccatt ttttgcagca tcattaaaga acagataccc gttatcttaa tatccgccac
      901 agaattgctc tatcatagaa accgcagaag acgtctgata cttctgtcag gtcatcgtaa
      961 ctcgtccagg tcagcggttt tgattacgga ttgatgatga aatgagcata agtgttgtac
     1021 ggttggatta tcaggatgtg ctatgaatat gatgcgtatt ttttatatcg gattgtcagg
     1081 tgtggggatg atgttctcat caatggcgtc tggaaatgac gctggcggac ttcaatctcc
     1141 ggcgtgcggc gttgtttgcg acccgtatat ttgtgttaac tcagatggca tttccccaga
     1201 gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac ttacaatcag tacaaggtta
     1261 cgatcccagc gaatttacat tctctaacgg tgtattttgc gatgttaaag aaaaattatg
     1321 tcgtgatgat cgttattttg gtgtgaatgg taagcggagt ggaaaaatca atcaaaccac
     1381 cacaaagatg ttatttatgt gtcgtgagtg atgtcttgtt tacattatgt tctattcgtc
     1441 attaacctct taagcgacag agaggagtgg ggcgttaacg tttatgacgg ggagagtccc
     1501 cgtcggttga tatttactta atttgaatat cgacgttata ttttttagtt ttgtttttac
     1561 gggcgtcgtt tcgggtttgc agcactcgca gctcatattt gccagaagca gggagtgagt
     1621 attgtccgtg actatcgagt tctggggaat atctggataa atcaacagaa tcatcgattc
     1681 ctggcccgaa gaggtaagta tcagcgcctt cattagagat actcacgtgt actttctgac
     1741 cttttttggc atagaaggta tatgtatcgt aatcgtatcc ctttatttcg ccggagtact
     1801 gagcgctgct atggcctttt ctgaactcaa cattgacatt cttacccgca gcaaagctcg
     1861 tggaggttag cagtgcaagc aacaatacag ccttactgat gcccttaatt ttcattggta
     1921 gtctcctgtt cagaatgagt acgatgaact gttataatat aacaatcctt cacgggagaa
     1981 ttccagtggc tttcctgtgt tcggttaaat cccggcgcgg catttttgcc ggggggatta
     2041 cttataaagg gggcatggac tgaacaaacc gtagaaatct acagctggct ggcttgtggc
     2101 gcgggtttca ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt
     2161 tttgtgtgat ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag
     2221 acgatttttc gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga
     2281 tgattcttcc gctggatggg cggaagaaac tggttaatgc cgatattgaa aaggttaaac
     2341 aggcattaac cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga
     2401 agcaacatct ttccgtcatg gggcaaaaaa cagacgacat taacaaataa ccgatatccg
     2461 gcggtggcat tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt
     2521 tatgtatcga catcccaact ggcacggttc gc
//
LOCUS       DAWDRC010000578         2547 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15143_length_2547_cov_1.556581, whole genome shotgun sequence.
ACCESSION   DAWDRC010000578 DAWDRC010000000
VERSION     DAWDRC010000578.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15143_length_2547_cov_1.556581"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    966..975
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          656 a          559 c          623 g          699 t
ORIGIN      
        1 ttgcatttgg caaaattttg aggcattttg ccggcatcat aggattttta atattacacc
       61 aactgctaat tatcgccaga aaagtcgctc aatttcacgg taattgtctg gttgcgcttg
      121 tctacaggtg gagtttacgt gttagctttt tgccgatctt cgttgaagtc aaaaaaatat
      181 ttcatcattt tactggcgct cgctgcaatt gccggactgg ggactcatgc cgcctggagt
      241 agcaatggtt tgccacgcat cgacaataaa acactggcca gactggcaca gcagcacccg
      301 gttgtcgttt tgtttcgcca tgctgaacgt tgcgatcgtt caaccaatca atgcttgtca
      361 gataaaacag gtattacggt caaaggtacc caggatgccc gagagctggg caacgctttt
      421 agtgctgata tccctgattt cgatctctat tccagtaata ccgtccggac tattcagtcg
      481 gcgacctggt tttcggcggg taaaaagctg acggtagata aacgatttct tcaatgcggt
      541 aatgagattt atagtgcaat taaagactta caacgcaaag cacctgataa aaatatcgtt
      601 atttttaccc ataatcattg cctgacatat attgctaaag ataagcgtga tgcgacattt
      661 aaacctgatt atctggatgg tttagtcatg catgtggaaa aaggcaaagt ttatctggat
      721 ggggaattcg ttaaccacta aattttcaat ctgacagcca gtgatggctg tcatcattgt
      781 taaagaagac ctttcaaacg taacgttttt cgggtggcaa cattcaaatc ataatgcacc
      841 agatcttcag gttttaccca cgcgtagtcc tgaaactctt cgtttatttt cacatctcgg
      901 ttggcagaaa cgcagtcaaa aatcaggtaa atcatataaa tctcttcctt gcgaccatct
      961 gcatannnnn nnnnntgcga atatcatcgc tgaaggtcca cggcgtgatt tctgtcaaaa
     1021 gcagctgttc tcccagttct tcgcgaattt cgcggcgtag tgcctcttca attcgttcgc
     1081 caggctccac gccgccaccc gaaagcgccc attgaccggg gaaaacgccg cgatcgtcgg
     1141 ccattttaca cagcaaataa gcaccatcat tttgaatcaa agggcataca atagtccgtt
     1201 gtcgcacagg ggtattcctt ataaataaaa agaaaccatg agaatacggt aactcagaaa
     1261 tatgtgaaag tgacagtaat ctcttttgcg atgataacta catttattat tatttttgtg
     1321 tgtatcgata aagtgtgttt attttttcgg cgtttaatta tttaagaagt atttagttac
     1381 aataagtgac tagatccctt ttttcgctca ttgaatcctc tgcgccaggc agtacaatcc
     1441 cgcagctttg ggaattttga ttaattgctt gtgggaaaga ggataatgaa aaaaattgcg
     1501 ctagcaggtc tggccggaat gctgttggtt tctgcatcgg tcaatgcaat gagcatcagc
     1561 ggccaggcgg gtaaagaata caccaatatt ggtgtcggtt ttgggactga atcgacgggc
     1621 ctggcgttaa gcggtaactg gacacataac gacgacgacg gtgatgccgc gggcgtgggg
     1681 ctggggttga atctgcctct cgggccatta atggcgaccg ttggcggaaa aggcgtttac
     1741 accaacccga acgatggtga tgaaggttat gcagcggcag ttggcggtgg tttgcagtgg
     1801 caaattggtg acagcttccg cgtgtttggt gagtattact actctccgga ttcgctctcc
     1861 agcggaatta aaagttatga agaagcgaat gctggcgcgc gttacaccat tatgcgtcca
     1921 gtcagcattg aggctggcta ccgttacctg aacctggcag gtaaaaatgg caatcgtgat
     1981 aacgccattg ctgatggtcc gtatgttggg gtaaatgcca gcttctgacg ccttccggcg
     2041 cggcgttctg tcgcgccatt cagtttctta ctacgccctg tctccctttg cgttatagtg
     2101 attcgataac aaaagcagga gataaccatg ttaaaagtgg aaatgttatc caccggggat
     2161 gaagtgttac acgggcaaat cgttgacact aacgctgcct ggctggccga ttttttcttt
     2221 catcaggggt tgccattatc tcgccgcaat acggtggggg ataaccttga tgacttagtc
     2281 accattcttc gcgaacgtag tcagcacgcc gatgtgctga tcgttaacgg cgggctggga
     2341 ccgaccagtg atgatttaag cgcactcgcc gctgcgacag caaaaggtga aggcctggtg
     2401 ctgcatgaag cctggctcaa agagatggaa cgctatttcc atggacgggg acgagtaatg
     2461 gcaccgagca accgtaaaca agcggagctg cctgccagtg ctgaatttat caataacccg
     2521 gtaggcactg cctgtggttt tgccata
//
LOCUS       DAWDRC010000579         2535 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15213_length_2535_cov_2.262903, whole genome shotgun sequence.
ACCESSION   DAWDRC010000579 DAWDRC010000000
VERSION     DAWDRC010000579.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15213_length_2535_cov_2.262903"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          592 a          712 c          715 g          516 t
ORIGIN      
        1 ggtgaacctg gcgctgtcgg atctggtgct cgcacaaacc ggtgctgctc cccgcagcgc
       61 tgtgttcagc ttctcccgtc agatgcaggg aacaggagaa atcgtcaccg cactgctcgc
      121 cgcattgctg gacggtgaga acatggatgt aatgctgcgt ggcgtctggc tcacatcctc
      181 gctacagcgt ggccaggtgg atgatatttt cacgcagtcc gccgcccgcc agtacggact
      241 gggtaacagc tcgctggcaa cctggcctct ggtggagacg acgccgtatt ttactcgccg
      301 cctcttcccg gaagtcctgc tggctgagcc gaacctggcg ggtgaaaaca gcgtctggct
      361 gaacagctcc cggcgcaggc tgaccgcctt ttccacctgt ggcgcggcac tggcaccatt
      421 attacaatca gaactggcag tctggcgtta acgtactggc acaagctaaa gcctttatgg
      481 acgtaccacc accgcaggga acggatgaat tcggcaatct gcaattgcca ttgcttaacc
      541 cggtacgcga tgccaccctg gcctatggtg attatcgcga tcacggtttt ctggcggata
      601 tgggattgta ccagggcgcc cgcgtagggc cgtatgtgga gcaaacctac attcagcttc
      661 ttgagcagcg ttatctcccc tcgttaatga acggcctgat ccgggatcta aacattgccc
      721 cgccagagag cgaagaaaag ctcgctgtgc tgcgcgtagt gcgcatgatg gaagacaaaa
      781 gtgggcgcaa caacgaggcg gtaaaacagt acatggcgcg gcgctggagc aatgaatttc
      841 acggccagcg cgatattcag gcgcaactga tggtgcatct ggactatgcg ctggagcaca
      901 ccgactggca cgcgcagcgc caaagcagcg acagcgatgc tgtaagccgc tggaccccct
      961 ataataaacc ggtcattaat gcgcaacatg aactgagcaa gctacccata taccagcgtg
     1021 tctaccagac ccttcgcacc aaagcattaa gcgtgttacc cgccgatttg aatttgcgcg
     1081 accaggttgg tcccaccttc gacaacgtgt tcgtcgccgg taatgatgaa aaactggtga
     1141 tcccgcagtt tctcacccgc tatggcctgc aaagctattt tatcaaacaa cacgacggtc
     1201 tcgttgagtt gaccgcgctg gattcgtggg tgctgaacct gacgcaaagc gtcgcctaca
     1261 gcgaggccga ccgtgaagag atccagcgcc atatcactga acagtacctc agtgactata
     1321 ccgctacatg gcgtgccgga atggataatc tcaacgtccg tgactatgag accatgccgg
     1381 cgctgaccga cgcgctggag cagattatca gcggtgatca gccattcctg cgtgctctga
     1441 cggcgctgcg cgataataca cacgcgctga cgctctccgg caaactggac gataaggcga
     1501 aggaagcggc gataaatgag atggattacc gcctgttatc ccggctgggg catgagttcg
     1561 cgccggaaaa tagcgcactg gaggagcaaa aggacaaggc gagtactcta caggccgtgt
     1621 atcagcaact gaccgagctg caccgttacc tgctggcgat ccagaactcg ccagtgtctg
     1681 ggaaatcggc gctgaaagca gtacagctac ggctggatca aaacagcagc gatccaatct
     1741 tcgccacccg tcagatggca aaaaccctgc ctgcgcctct taaccgctgg gtaggtaagc
     1801 tggcggatca ggcctggcat gtagtgatgg tggaagccgt tcgttacatg gaagtggact
     1861 ggcgcgacaa tgtagtgaaa cccttcaacg agcagcttgc cgataactat ccgtttaatc
     1921 cgcgcgccac acaggatgcc tcactggatt cgtttgaacg tttctttaaa ccggatggca
     1981 ttctggacaa tttctacaag aacaacctgc gcctgttcct tgaaaacgat ctgacctttg
     2041 gcgacgacgg cagaatgtta atccgtgaag atatccggca gcaactggat actgcgcaga
     2101 aaatccgcaa catcttcttc agccagcaga acgggctggg cgcacagttt gccgtggaaa
     2161 ccgtatcgct ttccggcaat aagcggcgca gcgtacttaa cctggacggc cagttagtgg
     2221 actacagcca gggacgcaac tacaccgccc atctggtctg gccgaacaac atgcgtgaag
     2281 gcaatgaaag ccagctgacg ctgattggca ccagcggcag agcaccgcgc agtatcgcgt
     2341 tcagtggacc gtgggcgcag ttccgcctgt tcggcgcggg ccagttgacc aatgtgacca
     2401 gtgacacctt taacgtgcgc tttaacgtgg acggcggcgc gatggtttac cgggtgcatg
     2461 tggataccga agataacccg atcaccggcg gtctgttcag ccagttccgt ttaccagata
     2521 cgttgtatta agggg
//
LOCUS       DAWDRC010000580         2533 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15224_length_2533_cov_2.365617, whole genome shotgun sequence.
ACCESSION   DAWDRC010000580 DAWDRC010000000
VERSION     DAWDRC010000580.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15224_length_2533_cov_2.365617"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          670 a          602 c          523 g          738 t
ORIGIN      
        1 aattatacat ttaactatct cctgtaataa atttcatcat gcaaatgagg tgtgccctcc
       61 ttaaattatc aatatcgtaa attccgccac tatattcaat cgcaacacga tgatacatgt
      121 ggaatttttt aacgatttct ttagtgccat aatgccaacc ttcaagtggt gcttttgcga
      181 ttaatccttg ttttatacgt gtttgattac cgcttgaaaa ctgcgctatc aactccggat
      241 actgactcac ctccagccaa agggtatcgg taaaagcggg aaaatgataa aatcacctaa
      301 tgaggcttca gtatttttac ctgtttttta agagcaatca cattattatt ttatgtcatt
      361 tatatcatcg cattagccag atgcgatgat gttccatata tagttttatt atctacttta
      421 tttagcgtct ttaaacaagg gtaacccctg tgaacgccgc cattctttga caattttcag
      481 gatgttttca ggcttatcat ctccttgact tttagggtag aaaattagat cgctccctga
      541 cggatgctgt gtaatttcaa taaaatgatc tactaatgaa tcccagtaca tttctagctc
      601 cttacctttt aaggattttc catctttcat agatgatctc aatccgccta aaaaattaag
      661 aaattcatcc tcggtataat cctggagctt ttctttaaac atatttatct cctgtattga
      721 atatcatcgt gtagtttagg ggtaacaatt cgtaaattat caatatcgta tacgctgccg
      781 ccatattcaa ctgcaacacg atgatgtatc tggaattttt taactatttc tttagggcca
      841 taataccaac cgtcaatagg tgcttttgcg gttaatcctt gttttatacg tgtttggtta
      901 ccacttgaaa actgcgctat caactccgga tcctgactca cctccagcca cagcgcctcg
      961 cgaaactcat caaaggtttt aaactcccgt ccgcgcaatt tatcagcaat atgcgccggg
     1021 attggtgcgc cgttgccgtt attggatacc gacatatcca gccagcgagt tcctggtgaa
     1081 agcaggacgc cgcgccctgt tacaacacca ggcagctttc gcggatcctc cttcaggtaa
     1141 acatagatgg gcttaatacc ggatcccgcc ggaaaaacca gaatataatc gttgaattct
     1201 ttcggatcag gaaccgggaa tggcggcgtg gtataggtat cggtgccgtc cggaatcggc
     1261 gtcaccagaa tggtgggctg ctccagcggc ggcctgtcgt taccggtatg gcccggaaca
     1321 tacccatgtt ccggattttc cggcgtccag gtaatcaggg ggccatcgac atcgtgagcg
     1381 ggggtaaatt cataacgctg cgtctgctcg ttccactgca tgttcgccac gcggacgttt
     1441 tcatacggcg taccttcacc cgtatgaaca ccatatacct ggggcttgcc gtgaatgtca
     1501 gttccccaga aaaaacgcac ccgggtagtg gcggtactgc ctgctagtgc tttctgccgc
     1561 agttcttcct cataccagaa ctcatcacgt ccaggcacac gagcactgcc ctcacccgca
     1621 gagggaacat acagagcaga tgccaacgca ccgatgtaag agccgcgggt tgcactgagt
     1681 acgccactga gcgaccattt tccccatatt tgagtcgcca acacactact cgggaacagt
     1741 agcatggttt gagcttctgc gggttgccca acatcgttag cctccggatg gtggtatcca
     1801 cagcagtcat caaccactgg cagagcaaaa atcgctactt gcccgaagtt ttcagcgggt
     1861 tcttctgccg ttccggcatc tgtacatccc ttctccttca gacaggattt ggcatagacc
     1921 ggtttaaccg ctggtggaga gagtggaagg ttaacaactg gtagcggctc agggttaaga
     1981 ttactggcag gtgggttttt aggtggaata aataagctat tatagccagc aggaaaggcc
     2041 aaccagtcaa attccgtatt ggctattaga tgtttgcata atatgtagtc ataattaaca
     2101 tttacacatt cggtaggaag attattgtca gaaatgttta catgaatagc agttaatgaa
     2161 gcatttctta gctgaatata ataataccgt tcccagcgcc cgcttttatt tatacgatac
     2221 aaatcgattt caataaataa ccgctcattc tgattgatgg cattagacag tagcggtgag
     2281 cttttatcaa tgagtttgca aaattgtagc ccctgtagat taacgccttt accagtactg
     2341 gtaatggcgt tggtgagtgc aaaggcaaaa atttcatctt catggccaac ctgccagcga
     2401 ttaccaaccg acgcatacgt cccacagcct gaagaaatat cgccctgctt ctcaccgatt
     2461 attctcaggt aaacaatatc gcccacttta tttcattccc gtttatcctt aacttttttg
     2521 cattaaatag aaa
//
LOCUS       DAWDRC010000581         2524 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15284_length_2524_cov_2.127177, whole genome shotgun sequence.
ACCESSION   DAWDRC010000581 DAWDRC010000000
VERSION     DAWDRC010000581.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15284_length_2524_cov_2.127177"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    824..833
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          700 a          616 c          533 g          665 t
ORIGIN      
        1 gattaactgg accgcaactg aactaacaat atcaggatga agcttaccgc ctcatcctga
       61 atggtcattt taccagtgcg ttaatgactg tttccatcgc ctgcttttcc agctcactgc
      121 gctgaatgcg tttattggct aatgctgtac gtaatacgcc tgcgataaca atatgctttg
      181 gttcctgccc cagatcgcgc atttccagca caactttacc gacaactctg cacatttctc
      241 ggtacagttc atcctctttt gtccggtttc ccattcttta tacctattta tcatgccaac
      301 tatcagcata tatcaatcta accaattaaa caaaaatcaa acaaaatcag acaaataacg
      361 tgataaatta ttttaactgt agcaattgag cgatgatgta tttatacacc ggatgaactt
      421 tcacttatcc tcacactgac aaccccggca ccagacgttg cgcaaacaac gaagtttttg
      481 cgtaaccttt tccctggaac gctgaatctt tgataacaat ttattgtcta acaagttgta
      541 tattttttga aacgctgttt ttgttttcct tttggattaa tttcagcgta taatgcgcgc
      601 caattgactc ttgaatggtt tcagcacttt ggactgtaga actcaacgac tcaaaaacag
      661 gcactcacgt tgggctgaga cacaagcaca cattcctctg cacgcttttt cgatgtcacc
      721 tatccttaga gcgaggcaca accactttcg taataccgga ttcgctttcc ggcagtgcgc
      781 ccagaaagca agtttctccc atccttctca acttaaagac taannnnnnn nnngactatg
      841 cagaacacgg tcagcgcatt cagaatctgc gcaacgtgat gagcaaaact ggtaaaaccg
      901 ccgctatcct gcttgatacc aaaggtccgg aaatccgcac catgaaactg gaaggcggta
      961 acgacgtttc tctgaaagcg ggtcagacct ttactttcac tactgataaa tctgttatcg
     1021 gcaacagcga aatggttgcg gtaacgtatg aaggtttcac tactgacctg tctgttggca
     1081 acaccgtact ggttgacgac ggtctgatcg gtatggaagt taccgccatt gaaggtaaca
     1141 aagttatctg taaagtgctg aacaacggcg acctgggcga aaacaaaggt gtgaacctgc
     1201 ctggcgtttc cattgccctg ccagcactgg ctgaaaaaga caaacaggac ctgatctttg
     1261 gttgcgaaca aggcgtagac tttgttgctg cttcctttat tcgtaagcgt tctgacgtta
     1321 tcgaaatccg tgagcacctg aaagcgcacg gcggcgaaaa catccacatc atctccaaaa
     1381 tcgaaaacca ggaaggcctc aacaacttcg acgaaatcct cgaagcctct gacggcatca
     1441 tggttgcgcg tggcgacctg ggtgtagaaa tcccggtaga agaagttatc ttcgcccaga
     1501 agatgatgat cgaaaaatgt atccgtgcac gtaaagtcgt tatcactgcg acccagatgc
     1561 tggattccat gatcaaaaac ccacgcccga ctcgcgcaga agccggtgac gttgcaaacg
     1621 ccatcctcga cggtactgac gcagtgatgc tgtctggtga atccgcaaaa ggtaaatacc
     1681 cgctggaagc ggtttctatc atggcgacca tctgcgaacg taccgaccgc gtgatgaaca
     1741 gccgtctcga gttcaacaat gacaaccgta aactgcgtat taccgaagcg gtatgccgtg
     1801 gtgccgttga aactgctgaa aaactggatg ccccgctgat cgtggttgct acccagggcg
     1861 gtaaatctgc tcgcgcagta cgtaaatact tcccggatgc caccatcctg gcactgacca
     1921 ccaacgaaaa aacggctcat cagttggtac tgagcaaagg cgttgtgccg cagctggtta
     1981 aagagatcac ttctactgat gatttctacc gtctgggtaa agaactggct ctgcagagcg
     2041 gtctggcaca caaaggtgac gtcgtagtta tggtttctgg tgcactggta ccgagcggca
     2101 ctactaacac cgcatctgtt cacgtcctgt aatattgctt ttgtgaatta atttgtatat
     2161 cgaagcgccc tgatgggcgc tttttttatt taatcgataa ccagaagcaa taaaaaatca
     2221 aatcggattt cactatataa tctcacttta tctaagatga atccgatgga agcatcctgt
     2281 tttctctcaa tttttttatc taaaacccag cgttcgatgc ttctttgagc gaacgatcaa
     2341 aaataagtgc cttcccatca aaaaaatatt ctcaacataa aaaactttgt gtaatacttg
     2401 taacgctaca tggagattaa ctcaatctag agggtattaa taatgaaagc tactaaactg
     2461 gtactgggcg cggtaatcct gggttctact ctgctggcag gttgctccag caacgctaaa
     2521 atcg
//
LOCUS       DAWDRC010000582         2521 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15301_length_2521_cov_2.175588, whole genome shotgun sequence.
ACCESSION   DAWDRC010000582 DAWDRC010000000
VERSION     DAWDRC010000582.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15301_length_2521_cov_2.175588"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2257..2266
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          528 a          733 c          638 g          612 t
ORIGIN      
        1 gccagtggca gcaacagctt cacgcacggt gttaaacacc ggcaggccga ggtgggtagt
       61 accgccttta cctggggtta cgccgccaac cattttagtg ccgtatgcaa tagcctgttc
      121 tgagtggaaa gtcccctggc taccggtaaa gccctggcag ataaccttgg tgtttttatc
      181 aattaaaatg gacattattt cccctccact gcggcaacaa cctgctgagc tgcatccgtc
      241 agaccttttg ctgcaataat attcaggccg ctgtcagcca gtttcttcgc gccgagttcg
      301 gcgttgttac cttccagacg taccacgacc ggtacgttaa cacccacttc cgctaccgcg
      361 ccgatgatac cgtcagcgat caggtcgcaa cgaacgatac cgccgaagat gttaaccaga
      421 acggctttca ctttgtcgtc agagaggatg attttgaacg cttcggttac acgttctttg
      481 gttgcgccgc cgccaacgtc aaggaagtta gctggttcgc cgccgtgcag tttaacgatg
      541 tccatggtgc ccatcgccag gcctgcgccg ttaaccatac aaccgatgtt accgtccagc
      601 gcaacgtagt tcagttccca ctgtgcagcc tgtgcttcac gcggatcttc ctgcgactgg
      661 tcacgcattt cgcgcagatc aggctggcgg aacagtgcgt tgccatcagc gcccagtttg
      721 ccgtcgaggc aaatcagatc gccctgtttg gtgatgacca gcgggttgat ttcgatcagc
      781 gccaggtcgc gctccaggaa aatggtcgcc aggcccatga agattttggt gaactgctga
      841 accagtttac cttccagacc cagtttaaac gccagctcgc gtccctgata cggcatcggg
      901 ccagtcagcg gatcaagcgc aactttgtgg atcaggtgcg gagtttcttc cgccactttt
      961 tcgatttcca cgccgccttc ggtggaggcc ataaagacca cgcgacgtga actacggtct
     1021 acaacggcac cgagatacag ctctttagcg atatcggtcg ctgcttcaac cagaatctgg
     1081 ttaaccggtt ggccattggc atctgtttga tacgttacca gacgcttgcc cagccagttt
     1141 tctgcaaaag cacggatgtc ttctttgctg tttaccactt tcacaccgcc cgctttaccg
     1201 cggccaccag cgtgaacctg acatttcact acccacggac cggcaccgat ttttgaagcg
     1261 gcttcttctg cttcgcgcgg agtagtacag gcataaccca ccggtgctgg taagccatag
     1321 cgggcaaaaa gttgttttgc ctgatattca tgtaagttca tgtgttctgt ccatccttca
     1381 gtaatcgtta tcttttaaac cgtagaccga cactctatcc acgtcggtct acagtgcagg
     1441 tgaaacttaa actactacac gtccagcagc agacgcgtcg gatcttccag caactctttg
     1501 atcgttacca ggaagcccac ggattcgcga ccatcgatca aacggtgatc gtaggacagc
     1561 gccaggtaca tcatcggcag gatctcaacc tggccattca ctgccatcgg acgatctttg
     1621 atagcgtgca tacccagaat tgcgctctgc ggtgggttga tgataggcgt tgacatcagg
     1681 gaaccgaaca caccaccgtt ggtgatggtg aagttaccac cggtcagatc ttcaaccgtc
     1741 agcttgccgt cacggccttt aacagccagc tctttgattt tcttctcgat gtctgccatg
     1801 ccgagggtat cgacatcacg cagaaccggc gtcaccaggc cgcgcggcgt agaaaccgcc
     1861 atactgacgt cgaaatagtt gtggtagacc acatcatcgc cgtcgataga cgcattcact
     1921 tccgggtaac gtttcagggc ttcaaccacc gctttcacgt agaaggacat aaagcccaga
     1981 cgaatgccgt ggcgtttttc aaacgcttca ccgtactgct tacgcagatc cataatcggc
     2041 ttcatgttga cttcgttgaa cgtggtcagc atggcggtgg agtttttcgc ttccagcaga
     2101 cgctctgcca cacgcttacg caggcgagtc atcggcacac gtttttcgct acgtgcagcc
     2161 agggtcggtt gcgccgccgg agcagccgct gccggagcag actctttcgc cggggctttc
     2221 gctagatgtt tttccacatc ttcacgggtc agacgannnn nnnnnncacc cacaccggtg
     2281 cctttaatgg cgctggcgtc gaggttatgt tcagccagca ggcgacggat cgccgggctt
     2341 aacgcatcgt tgttttgctc ttccagagac gcctgctggc gttgcgccgg agtggacgct
     2401 ttctcttcag atttcgcgct ggtttcttta cccgcgctgt tgccttcacg caggcgacca
     2461 aggatctgac gagacgttac cgttgtacct tcatcttcca gaaccgcaga cagaatgccg
     2521 g
//
LOCUS       DAWDRC010000583         2518 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15324_length_2518_cov_2.306537, whole genome shotgun sequence.
ACCESSION   DAWDRC010000583 DAWDRC010000000
VERSION     DAWDRC010000583.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15324_length_2518_cov_2.306537"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          579 a          758 c          658 g          523 t
ORIGIN      
        1 acatccggcg acaccgcgcg ggtggcagtt tgcccgccgc gtaatcccac ctcttcgctg
       61 gaacttgcca ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgcagtaac
      121 gtcaccagca gatagcaacc gaggcggtca tcaaaggctt tccccatcac ccgctggtga
      181 gggagaacct gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcctgcctgc
      241 atcacttcgt cacaggagcg cgcgccaatg tccacgcgca tggcgctgac gtcattcccc
      301 tgccggtcgc cgtcaagcag gcctggaatt ttgcactctt cacgggtagt gatacgcacc
      361 gactgaagct ggcgggcagc catgcgtaca ttgccaaccg gcaacacatc gatcgcccct
      421 tcgcgggaga tgctgcgcac cataaatccc acttcgtcca tatgcgcaca gatcatcacc
      481 ttcggacctg tcgactcatt gagtcggatc agcaccgatc ccagcccatc aaatcgcact
      541 tctttttgca ggcgatccgc ttcttcccgt aggatctgcc gtacttcctg ttctgaggag
      601 gcgatcgcat ctgcctcgct caacgctttt aagagcgata aatccattat gcctctcccg
      661 tgagcaacac tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtgaccagca
      721 cgacatcttc gatgcgcacg cccccttgcc ctggcaaata aatccctggc tccacggtca
      781 gtaacatacc tggctgtagc gtcgtggtgt cccgcggtga aaaacgcggc tcttcatgga
      841 cttcaatacc gatagcatga ccggtgttat ggccgaaaaa cttgccataa ccggcctcgg
      901 taatcacgcg gcgcgcggca tcgtcaacct gctggcagcg cacgccgggg cgaattacag
      961 agattgccgc gagctgtgcc tgcaggacta tctgatagac gttaaacagc gggtgagatt
     1021 cggcgctcac cccttcgcca ttcaccagca aggtgcgcgt catatcagag cagtagccct
     1081 gatacagcgc accgaaatcg agagtgacaa actcgcccgc tgcaacaatc ttgtcgctgg
     1141 ctttaccgtg cggcagcgcc ccacgcaagc cactggcgac aatggtgtca caagaggctt
     1201 tttctgcgcc ctgctggcgc ataaaccact ccagttcagc ggctatctcg cattcgctca
     1261 tacccgcctg aataaagcgg cgaatatgct ctgcgccacg atcggcaatc ccgcaggcaa
     1321 ggcggatttt ctccacctct tctggcgttt tgatttgccg cagcacatcc ggcgtggcgc
     1381 tcaccagttt cgcattgagt tcgaactgcc agcggtgcgc ggtttcccaa ctcacctgct
     1441 ggccttcaaa accgagcgtt tgcaactgct catcggcaat gatttgtctg acgatggtgg
     1501 caagcgtgtg catcgcgtcg agcaaatgca gctggtaacc ttgcgtgcgg gcttctacat
     1561 ccgcgtaata gcgcgaatcc accagaatgt gcgcactttc acggctaatc accacataac
     1621 ctgatccggt ggagatcccc agatgcggct gtttgttctg ccgtgaggag agaagcaccg
     1681 catccagttg ttgtgcctta agccagtcac gcagcgaagc gagtaatgtc atctggattt
     1741 ttccttctta caggctatcg attaacaatt tgcctttacg gaacatcatc agacgcagga
     1801 acaccaccat cagcgcagta atgattgctc ccagcgcgat ccccgccata tagacgccga
     1861 ggttagtgac cagcggccac gcccagatag cggattccgg gaaccattgc actgcgccca
     1921 gccagacagc agcggtggaa ccgacaatcg cgccaaccat ataggacgga atggcggtga
     1981 tagggctttc cagtgcaaac ggaatcgcac cctcactaat ccccataaac gccaggaaca
     2041 tcgcggtctt accctgcgga taaagttgag cgttgaacag acgtttgccg gttaaacgac
     2101 ggtcaataat ggttgccaga cccagaccaa tcggcggaat aacgatagcg atagaacgtg
     2161 cggtgaccgg taatacgtgg tcagtggtaa agctgaaggc aacgaaacct gccgctttgt
     2221 tgatcgggcc accaaggtcg attgctgtcg ccgcggcgat ccccattgcg tacatcagcg
     2281 cgcccttctc acctgctgcg gttagcacgg tacggatacc gccgttgatc cagccaccaa
     2341 agggggtgat gacgtagtac atcgccagca tgacaaaaat ggcggaaaga atcggtagca
     2401 aaaatgtggt tttgaacgct aacaggaaat ccggtaactg gattttctgg ttcatccact
     2461 tcacaaggta accggcaacg atagaaataa tcagcgcacc aatgaaggtg gacggcac
//
LOCUS       DAWDRC010000584         2515 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15341_length_2515_cov_1.968699, whole genome shotgun sequence.
ACCESSION   DAWDRC010000584 DAWDRC010000000
VERSION     DAWDRC010000584.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15341_length_2515_cov_1.968699"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    214..223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1700..1709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          544 a          663 c          755 g          533 t
ORIGIN      
        1 cgtctgggtt ttcgcgctgt tctcgacagc accattgcgc tggcagtgtg gctgcaaatt
       61 gaactggcgg aaccgtggca gccgtggctg gcggatatcc gttcgcgtct tggcaacatt
      121 atgcgtgccg atgcgctggg gaaaccgctg gcctgccagg cgattgtcgg gctttctgac
      181 gaagatctgc atcggctttc tcaccagccg ctgnnnnnnn nnntgttacg caccaaagtg
      241 cgggaaaccg aaaccgtcgc ggcgcaggtc tttatcaccc gcagctttga agtattacgc
      301 ccggacattt tgcaggcgct gaaccgcctt tccagcacgg tctacgtgat gatgatcctg
      361 agcgtaacga agcagccgct aacggtgaag cagatccaac aacgactggg ggaaacgcaa
      421 tgattattga acgttgtcgt gaactggcgt tgcgagcgcc cgccagagta gtttttccgg
      481 atgcgttaga tcaacgtgtg ctgaaagctg cgcaatattt acatcaacaa ggtctggcaa
      541 cgcccattct ggtcgccaat ccgtttgaac ttcgtcagtt tgcgctcagt catggcgtgg
      601 cgatggacgg gctacaggtg attgatccgc atggcaacct cgcaatgcgg gaagaatttg
      661 ctcatcgctg gctggcccgc gcgggcgaaa aaacgccgcc ggatgcgctg gaaaaactta
      721 ccgacccgct gatgttcgcc gccgcaatgg tcagcgccgg taaagcggat gtctgtatcg
      781 cgggcaacct ctcttccacg gcgaatgtgc tgcgtgccgg attacgcatt atcggcttgc
      841 agccaggctg taaaacgctc tcatccattt tcctgatgct gccacagtac agcggtccgg
      901 cgttgggctt tgccgattgc agcgtggtgc cacagccgac ggcggcacaa ctggcggata
      961 tcgcgcttgc cagcgccgaa acctggcgcg ccatcaccgg agaagaaccg cgcgtggcga
     1021 tgctgtcgtt ttccagcaac ggtagtgccc gtcacccctg cgttgccaac gtccagcagg
     1081 cgacagaaat cgtccgtgag cgcgcaccaa agctggttgt cgatggcgag ttgcagtttg
     1141 acgccgcctt cgtgccggaa gtggcggcgc aaaaagcgcc tgccagcccg ttacagggca
     1201 aagccaatgt gatggttttt ccgtcgctgg aggcaggaaa tattggctac aaaatcgcac
     1261 aacgacttgg cggatatcgt gccgtcgggc cattgataca aggacttgcc gcgccgatgc
     1321 acgatctctc tcgtggttgt agcgtgcagg aaattatcga actggcgctg gtggcagctg
     1381 tgccgcgtca gacagaagtg aaccgcgaaa gcagtttaca aacactggtt gaatgaacgg
     1441 tcccgttctg gaccctgata aagaggaaag cacaatggaa gcattaggaa tgatcgaaac
     1501 ccggggcctg gttgcactga ttgaggcttc tgacgcgatg gttaaagctg cgcgcgtcaa
     1561 gctggttggt gttaagcaga ttggcggcgg cctgtgtact gccatggttc gtggtgatgt
     1621 tgcagcctgt aaagccgcga ccgatgccgg tgccgcagcg gcacagcgta tcggcgagct
     1681 ggtatctgta cacgttatcn nnnnnnnnnt atcccgcgtc cgcacggcga cctggaagaa
     1741 gtcttcccga tcggcctgaa aggcgatagc agcaacctgt aatcaccttg tgtgacgtcg
     1801 attcgggcgt cacgctctct cctttttcat tttaccttct gcggagggta gggagagctt
     1861 ttacctgaaa tacgccacgg aggcgggtat gaaactggca gtcgtcactg gacaaattgt
     1921 ttgtaccgta cgccatcacg gactggcgca tgacaaattg ctgatggtgg aaatgattga
     1981 tccacaaggt aatcctgacg ggcaatgcgc cgtcgccatc gacaatattg gcgcgggaac
     2041 cggggagtgg gtgttactgg tgagtggcag ttccgcccgc caggcgcata aaagcgaaac
     2101 gtcaccggtc gatctgtgcg tgattggcat cgtcgatgag gtggtgtctg gcggtcaggt
     2161 cattttccac aaataaggca gaacatcatg aatcaacagg atattgaaca ggtggtgaaa
     2221 gcggtactgc tgaaaatgca aagcagtgac acgccgcccg ccgccgttca tgagatgggc
     2281 gttttcgcgt ccctggatga cgccgttgcg gcagccaaag tcgcccagca agggttaaaa
     2341 agcgtggcaa tgcgccagtt agccattgct gccattcgtg aagcaggcga aaaacacgcc
     2401 agagatttag cggaacttgc cgtcagtgaa accggcatgg ggcgcgttga agataaattt
     2461 gccaaaaacg tcgctcaggc gcgcggcaca ccaggcgttg agtgcctctc tccac
//
LOCUS       DAWDRC010000585         2513 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15355_length_2513_cov_2.469487, whole genome shotgun sequence.
ACCESSION   DAWDRC010000585 DAWDRC010000000
VERSION     DAWDRC010000585.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15355_length_2513_cov_2.469487"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    228..251
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2160..2169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          629 a          657 c          691 g          502 t
ORIGIN      
        1 ctcatgaaaa tcaaaactat tttaacacca gtagcctgtg ctctgctgat gagcttttcc
       61 gcccatgccg ctaacgccga caattataaa aacgtgatta accgtactgg cgcgccgcag
      121 tacatgaagg attacgatta cgacgatcac cagcgtttta atccgttttt cgatctcgga
      181 gcctggcatg gtcatctgtt gccagacggc cctaacacca tgggcggnnn nnnnnnnnnn
      241 nnnnnnnnnn nctgctgacg gaagaataca tcaactttat ggccagcaat ttcgatcgcc
      301 tgaccgtctg gcaagacggc aagaaagtcg acttcacgct ggaggcatac agtattcccg
      361 gcgcgctggt gcaaaagttg atatcgaaag atgtacaggt cgaaatgatt ctgcgcttcg
      421 ccacgccgcg cacatcacta ctggaaacca aaatcaccag cgataaaccg ctggatctgg
      481 tgtgggatgg cgaactgctg gaaaaactgg aagcgaaaga agggaaaccg ctttccgata
      541 aaaccattgc tggcgaatac cctgactatc agcgcaaaat cagcgccacc cgtgatggcc
      601 tgaaagtcac ctttggcaaa gtgcgcgcca cctgggatct gctgacctcc ggtgaatcag
      661 aatatcaggt gcataaatcc ctgccggtgc agactgaaat caacggtaat cgctttacca
      721 gtatggcgca tatcaacggt tcgacgacgc tctacaccac ctattcccat ctgctgaccg
      781 ctcaggaagt tagcaaagag caaatgcaga tccgcgatat tctggcgcgt ccggcgtttt
      841 atctcaccgc ctcgcagcaa cgctgggaag agtatctgaa gaaagggtta accaatccgg
      901 atgcgacgcc ggaacagacg cgcgtcgcgg tgaaagccat cgaaacgctc aacggtaact
      961 ggcgatcgcc gggcggtgcg gtgaaatata acaccgtcac gccgtcggtg accgggcgct
     1021 ggttctccgg caatcagacc tggccgtggg atacctggaa gcaggcgttt gcgatggcgc
     1081 atttcaatcc ggatatcgcc aaagagaata tccgcgcggt cttctcctgg cagatccagc
     1141 caggcgacag cgtgcgtccg caggatgtgg gctttgtccc cgacctgata gcgtggaacc
     1201 ttagcccgga gcgtggcggc gatggcggca actggaacga acgtaacacc aaacccagcc
     1261 ttgccgcctg gtcggtgatg gaagtgtaca acgtcaccca ggataaaacc tggctggcag
     1321 agatgtaccc gaaactggtg gcctatcaca actggtggtt acgtaaccgc gatcataacg
     1381 gcaacggcgt gccggaatat ggcgcgaccc gcgataaaac ccacaacact gagaatggcg
     1441 agatgctgtt tacagtgaaa aaagataaca aagaagagac gctgtctggg ctgaacaact
     1501 acgcccgcgt ggtggagaaa ggccagtacg acagtctgga aatcccggca caagttgctg
     1561 cgtcgtggga atcaggtcgt gatgacgccg ccgtctttgg gtttatcgac aaggaacagc
     1621 tggataaata tgtcgctaac ggcggcaaac gtagcgactg gacggtgaaa ttcgcggaaa
     1681 accacagtca ggacggaacg ttgctgggct actcgctatt gcaggagtcg gtggatcagg
     1741 ccagctatat gtacagcgat aaccattatc tggcggagat ggcgacgatt ctcggtaagc
     1801 cggaagaggc caaacgctat cgtcagttag cacaacagct cgcggactac atcaacacct
     1861 gtatgttcga cccgactaca cagttctact atgacgtgcg tattgaagat aaaccgctgg
     1921 cgaacggctg cgcgggcaaa ccgattgtcg agcgcggtaa agggccggaa ggctggtcac
     1981 cgctgtttaa cggtgcggca acgcaggcca acgccgacgc ggtggtgaag gtgatgctgg
     2041 atccgaaaga attcaacacc tttgtcccgc tggggacggc agccttaacc aacccagcct
     2101 ttggcgcaga tatctactgg cgcgggcgcg tatgggtgga tcagttctgg tttggtctgn
     2161 nnnnnnnnnt ggtctgaaag ggatggagcg ttacggttat cgcgatgacg ccctgaaact
     2221 ggctgatacg ttcttccggc acgccaaagg gttaaccgcc gatggcccga ttcaggagaa
     2281 ttacaacccg ctcactggcg cgcagcaagg cgcaccaaat ttctcctgga gtgccgcgca
     2341 tttgtatatg ttgtataacg atttttttcg taagcagtaa ttagcaacgg ctggcggatg
     2401 cggcgtaaac gccttatccg ccctacatgt gcaatccccc aggccggata agacgcgtca
     2461 tgaactgaac cccaaaacct ggacagtttc agttaaccgg ctttttgata ctc
//
LOCUS       DAWDRC010000586         2508 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15387_length_2508_cov_2.160620, whole genome shotgun sequence.
ACCESSION   DAWDRC010000586 DAWDRC010000000
VERSION     DAWDRC010000586.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15387_length_2508_cov_2.160620"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    897..906
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          712 a          583 c          619 g          584 t
ORIGIN      
        1 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttcttag
       61 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcagggatgt
      121 tctgttctgc gttggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
      181 aaccagggcg caaaatcctc atgtctggcg gtgggcgctg caactttacc aacctttatg
      241 tcgaaccggg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcgcgtt
      301 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcctgg cacgagaaaa
      361 cgttagggca gctcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
      421 agtgcgagaa gggcaatgtg gccttcagat tgcgtagcga agtgctgagc gtggcgaagg
      481 atgatacagg cttcacgctt gaactgaacg gcatgactgt cggttgcgaa aagctggtca
      541 ttgcgaccgg tggactgtca atgccggggc tgggtgcatc gccgtttggt tataagattg
      601 ccgaacaatt cggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
      661 ataaaccgtt gctcgaggag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
      721 ccgctgaaaa cggtatcgtt ttccgtgaga acttactctt cacccaccgc ggcttatctg
      781 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agcatcaatc
      841 tgctaccgga tgtggacctc gaaaccttcc tgaatgagca gcgtgatgca catccgnnnn
      901 nnnnnncggt tacaacaacc cgggcaaatc ccggatgtct cgctaaaaca gctcaacgtg
      961 cgtgaccaac agacactgat tagcacattg actgactggc gcgtacaacc caacggcact
     1021 gaaggctatc gcactgccga agtgacgctc ggcggcgtgg acaccaacga actctcttca
     1081 cggacgatgg aagcgcgcaa agtgcctggg ctgtacttca tcggcgaagt gatggacgtc
     1141 accggctggc tggggggcta taacttccag tgggcgtggt cgagtgcatg ggcttgtgcg
     1201 caggatttgg tggcagaata agaggcaaaa tgcgctctcg ataccagagg gattaacctg
     1261 aataaaacca gataaggcgt ccaacgcctt atctgaagcc ttttttatta ttttttcgct
     1321 gaacccgctt ctaatataga aacgatggaa aacagtgtaa aacaaactgc acctaagcca
     1381 accagaactc gcgaaggaat aaagtaagcc atatcggttt gcgcaatttc agccaggaac
     1441 gcagcaagga acagacaaaa cagacaggta aataccggaa tcatgggtat tctgttggct
     1501 aacgaacagg tgcgtcgcca taccagcgct aacaaccaca ctttggagaa aatactgtag
     1561 cagatcatcc ccaggcaaat gagaataatg ccgggggcca ggcgggcgct tgcatctgaa
     1621 ctgattaata cgtagatacc aaaaataatg gtaagtgagc caagcaaaat aacccaatag
     1681 caccacagcc aatgttcttt cactgagaac gtattccgcg tttgatggac aatagtggcg
     1741 acaaggccaa taagacaagc gcaaattgcc gttaacccca ttagtacgtg acctgcaaca
     1801 taatgtggtg tgatatctgc actacgtaac agtatgactg accaaatgaa accaaagaga
     1861 gtaagcagaa cgggaacggc aattaagcaa ttaccgattg ttgaagaata ggcctgcaat
     1921 ggcgtaccgt cactcttact gcccgaggcg tttttgggga tcaacagaaa atgtccggaa
     1981 gatgccgcta cggttgatac acaagcggca atcatgccaa cgccgaaaat aacatgaccg
     2041 gcgacaaatt catctgccat cacattattg cttgccaaca gtgaccatcc ccaaatcata
     2101 gttgtagcag aaccagcata accaataaca ggaaataacc tgttgtagaa tttattcatg
     2161 ccgtgagtta actgagatat aataataaat gcggtagtga acaatgccag acatatcgca
     2221 gaaagagaaa tcagaacatg ccctgcaacg aaatattcac tttgcccaga accggttaat
     2281 acaaactcac ctaatgcgat gcatattata cccatcagta aaggaattaa tttaaacaac
     2341 catccgatat atatattcat tttaaacccc atatatattt tctgctacac ttcgaaaaac
     2401 atatgattcg tcttttcagt tagttaaata tgtctataag atgccatcaa ttaaaaaagt
     2461 aacttcacta taatcggatt tagcgttgtg gggatcaact tttagcgg
//
LOCUS       DAWDRC010000587         2502 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15417_length_2502_cov_2.461790, whole genome shotgun sequence.
ACCESSION   DAWDRC010000587 DAWDRC010000000
VERSION     DAWDRC010000587.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15417_length_2502_cov_2.461790"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    791..800
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1839..1848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          556 a          645 c          683 g          598 t
ORIGIN      
        1 gaggatgaaa cgctgctggc cggagttctc tgtacatttt tggcaacgga agaacagctc
       61 accaatgcct caacgtctat cagcgttccg gtgaacagga acagcacgaa cagaatttgc
      121 aggcaaatat tctccacggt ctggagcagc gtaggtttca atttaaatac ccccggatag
      181 tcctctgagt gcttcccttt tcattaagtt tttaattatc gttgcaatac cttaatgata
      241 ttggtaatta ttatcattag catttgcgct aacgttgtct ctggtaaggc tgaaagggaa
      301 cacgcatcgc cagtacaagg tcattttact tacaggccag cgaaggcgag actatcagga
      361 tgatgcaagc cgtgtgtcta aaaagtcacg gatctttcca gattcattgt cggtctaaca
      421 ctgcacaaat gcgtaagaac atcccggcgt agctgtctga cgccgggatg ttcaacagga
      481 caatggcgcg taagcgttaa tggaagtggg gatacggagt tccctgttca cctgtgtatg
      541 tcacggaaaa gtctctgact gatctgtcca taacgctggt gaagcacaaa tggcgcagca
      601 tctggtaaag caggtttaac gaaaagtcgt gtcctgctgc tggcatatga gtgcgtcgac
      661 tgcctgattt agatcgtcaa gcggagaggg attttctcat gcgtattttg tttgtaggcc
      721 cgcctctcta tgggctgttg tatcctgtgc tgtccctggc gcaggcattt cgcgttaacg
      781 gtcatgaagt nnnnnnnnnn tgtttgacgc tgcccccggt ctggattccg aagctggtta
      841 ccgccaccat gaggcacagc ggaaaaaaag taacatcgga acccaaatgg gcaacttctc
      901 attcttcagc gaagaaatgg cagaccatct ggtggagttt gccggacact ggcggcctga
      961 cctcatcatc taccctccac tcggagtcat cgggccgctg attgccgcca aatatgatat
     1021 accggtcgtg atgcaaaccg taggcttcgg ccataccccc tggcatatca ggggggtaac
     1081 gcgttcgctc acggacgcct atcgtcgcca caatgtcggg gccacaccac gcgatatggc
     1141 ctggatcgac gtaacaccgc cgagtatgag cattctggaa aacgacggtg aacccattat
     1201 tccgatgcag tacgtcccat ataacggtgg tgcggtgtgg gaaccatggt gggagagaag
     1261 gcccgatcgt aaacgtcttc tggtgagtct cggcactgtc aaaccgatgg ttgacggcct
     1321 tgatctaatc gcctgggtta tggattctgc cagtgaggtc gatgccgaga tcatcctgca
     1381 tatttcagcc aatgcgcggt ccgatttgcg ctcgctgcca tcaaatgttc gcctggttga
     1441 ctggataccc atgggtgtct tcctcaacgg cgcagatggt ttcattcatc atggcggagc
     1501 aggcaatacc ctgacagcgc tacacgctgg tattccacaa atagtcttcg gccagggagc
     1561 cgatcgcccg gtaaatgccc gcgttgttgc cgaacgcggc tgcgggatta tccccggcga
     1621 cgtcggcctg tcgagtaaca tgatcaatgc tttcctcaac aatcgctcac ttcgcaaagc
     1681 ctctgaagag gtagcagcgg aaatggcggc gcaaccctgc cctggtgaag tggcgaaaag
     1741 cctgatcaca atggtacaga aagggtagcc actacacctt catgataatc atttgccagc
     1801 ccccatccag gagtcatgca tccataacaa tgcgggacnn nnnnnnnntt ttgcggaacg
     1861 atccggccag tggatcgtat tttacagacg gaggaggctt aatgcctgcg aatcacactc
     1921 ccacaccggc tcagtcatgg atagttcgcc tggcgcgcgt gtgctgggaa cgtaagaaac
     1981 ttagtgtcat cgtggtggta gcgtcagtat cgactatttt gctggctgcg ctgacgccac
     2041 tgctgacaag acaggctgtg aatgacgcac tggcgggcaa tccggcccgc ctgccgtggc
     2101 tggcctgcgg gttactgttg atcgcttttt ttgatttcat cggtaactat gtgcgccgtg
     2161 gttatgccgg gatgctctca ctctgggtgc agcataccct cagaggacgg gtattcgaca
     2221 gtattcaaaa acttgacggc gcaggccagg atgcgctgcg caccgggcag gtgatttcac
     2281 ggaccaacag cgatctgcag caggtgcata ccctgctgca gatgtgcccg gtgccgctgg
     2341 cagtgttcac ttattacatt gccggcattg ccgtgatgct gtggatgtcc cctgccatga
     2401 cgcttatcgt cgtgtgcgta ctggtatgct tggcgatcac cgcgcttcgt gcgcgtcgta
     2461 gggtcttcgc gcaaaccgga atggcatcgg accaactggc ga
//
LOCUS       DAWDRC010000588         2500 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15428_length_2500_cov_1.547648, whole genome shotgun sequence.
ACCESSION   DAWDRC010000588 DAWDRC010000000
VERSION     DAWDRC010000588.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15428_length_2500_cov_1.547648"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    2175..2184
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          586 a          637 c          700 g          567 t
ORIGIN      
        1 ttctaccgcc agtgcgtttg tcgccgcggc ctgtgggctc aaagtggcga aacatggcaa
       61 ccgtagcgtc tccagtaaat ccggctcgtc ggatctgctg gcggcgttcg gtattaatct
      121 tgatatgaac gccgataaat cgcgccaggc gctggatgag ttaggcgtct gtttcctctt
      181 tgcaccgaaa taacccaccg gattccgccg gttcgccagc aactaaaaac ccgcacactg
      241 ttcaatgtgc tggggccatt gattaacccg gcgcatccgc cgctggcgtt aattggtgtt
      301 tatagtccgg aactggtgct gccgattgcc gaaaccttgc gcgtgctggg gtatcaacgc
      361 gcggcggtgg tgcacagcgg cgggatggat gaagtttcat tacacgcgcc gacaatcgtt
      421 gccgaactgc atgacggcga aattaagagc tatcaattga ccgctgaaga ttttggcctg
      481 acaccctacc accaggagca actggcaggc ggaacaccgg aagaaaaccg tgacatttta
      541 acacgcttgt tacaaggtaa aggcgacgcc gctcacgaag cagccgtcgc ggcgaatgtc
      601 gccatgttaa tgcgcctgca tggccatgaa gatctgcaag ccaatgcgca aaccgttctt
      661 gaggtactgc gcagtggttc cgcttacgac agagtcaccg cactggcggc acgagggtaa
      721 ataatgcaaa ccgttttagc gaaaatcgtc gcagacaagg cgatttgggt agaagcccgc
      781 aaacagcagc aaccgctggc cagttttcag aatgagattc agccgagcac acgacatttt
      841 tatgatgcgc tacagggggc acgcacggcg tttattctgg agtgcaaaaa agcgtcgccg
      901 tcaaaaggcg tgatccgtga tgatttcgat ccggcacgca ttgctgccat ttataaacat
      961 tacgcttcgg caatttcagt gctgactgat gagaaatatt ttcaggggag ctttgatttc
     1021 ctccccatcg tcagccaaat cgccccgcag ccgattttat gtaaagactt cattatcgat
     1081 ccttaccaga tctatctggc gcgctattac caggccgatg cctgcttatt aatgctttca
     1141 gtactggatg acgaacaata tcgccagctt gcagccgtcg cccacagtct ggagatgggt
     1201 gtgctgaccg aagtcagtaa tgaagaggaa ctggagcgcg ccattgcatt gggggcaaag
     1261 gtcgttggca tcaacaaccg cgatctgcgc gatttgtcga ttgatctcaa ccatacccgc
     1321 gagcttgcac cgaaactggg gcacaacgtg acggtaatca gcgaatccgg catcaatact
     1381 tacgctcagg tgcgcgagtt aagccacttc gctaacggct ttctgattgg ttcggcgttg
     1441 atggcccatg acgatttgaa cgccgccgtg cgtcgggtgt tgctgggtga gaataaagta
     1501 tgtggcctga cacgtgggca agatgctaaa gcagcttatg acgcgggcgc gatttacggt
     1561 gggttgattt ttgttgcgac atcaccgcgt tgcgtcaacg ttgaacaggc gcaggaagtg
     1621 atggctgcgg caccgttgca gtatgttggc gtgttccgca atcacgatat tgccgatgtg
     1681 gtggacaaag ctaaggtgtt atcgctggcg gcagtgcaac tgcatggtaa tgaagatcag
     1741 ctgtatatcg acactctgcg tgaggctctg ccagcacacg tcgccatctg gaaggcttta
     1801 agtgtcggtg aaactcttcc cgcgcgcgat tttcagcaca tcgataaata tgtattcgac
     1861 aacggtcagg gcgggagcgg acaacgtttc gactggtcac tattaaatgg tcaatcgctt
     1921 ggcaacgttc tgctggcggg gggcttaggc gcagataact gcgtggaagc ggcacaaacc
     1981 ggctgcgccg ggcttgattt taattctgct gtagagtcgc aaccgggtat caaagacgca
     2041 cgtcttttgg cctcggtttt ccagacgctg cgcgcatatt aaggaaagga acaatgacaa
     2101 cattacttaa cccctatttt ggtgagtttg gcggcatgta cgtgccacaa atcctgatgc
     2161 ctgctctgcg ccagnnnnnn nnnngtgcgc aaaaagatcc tgaatttcag gctcagttca
     2221 acgacctgct gaaaaactat gccgggcgtc caaccgcgct gaccaaatgc cagaacatta
     2281 cggctggcac gaacaccacg ctgtatctga agcgcgaaga tttgctgcac ggcggcgcgc
     2341 ataaaactaa ccaggtgctg ggccaggctt tactggcgaa gcggatgggt aaaactgaaa
     2401 ttattgccga aaccggagcc ggtcagcatg gcgtggcgtc ggcccttgcc agcgccctgc
     2461 tcggcctgaa atgccgtatt tatatgggtg ccaaagacgt
//
LOCUS       DAWDRC010000589         2497 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15443_length_2497_cov_2.111794, whole genome shotgun sequence.
ACCESSION   DAWDRC010000589 DAWDRC010000000
VERSION     DAWDRC010000589.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15443_length_2497_cov_2.111794"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    600..609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          572 a          676 c          644 g          595 t
ORIGIN      
        1 agtggctgcc gccggaattg ccctgaacat taaaaggtgg atgagtcggg ccatttagcc
       61 ttgaatactg gcgtctcacc cctggcaata atgaacgtaa taatcgggta ttaattgaaa
      121 aacgtaagca gctttccgac agccttctgt gggctaatgg cattgcccct tcagaggggc
      181 ttgaggatga cgacctgctg caagcgctta tcgacgaatt actcaccctc gttgacggtt
      241 atctggcacc tttcgctcct gtggtggcga atattctaaa aaactggatg accgcatgac
      301 ataacggaca acggggcctg atggccccgt tgtgtggtgt atacgctaca gctcgttgag
      361 cgtgtactgg aagcggcagg tggaatcaat cggctggcgg ctgctggtat cgacacgcac
      421 taccatgtcg tgcagttgct tgtcagtgat gggatgctgg ttgggtgtga ccgtcacttt
      481 cgaactgtca cagccggagg tggagaaggt gatcatctgc gccacaaagc ggcagtatgc
      541 gcctcgatcg tccatcacgc tgccgtcaca gagcttgccg ctttccatca aaaaggtggn
      601 nnnnnnnnna aaaggtgggc agcgttttct caatcgttcc acccgtcgat tgcagcgaca
      661 gcactttcga atggacaaca taatcttcac tccacgaaat atcataagcg gcgacggaga
      721 cggggcatga tgagttgtga actttggggc caatatatag accaacacca tctaccgaag
      781 agatattgct gtcagtatat gtcgagatca accacgggca accgtgcacc tcaccatcat
      841 agtacactcc gaggctggcg ttgtaacaga tccaatcaga tttactcgac cgagcatcat
      901 acgcgtcaag accaacacag gtagattttg aagcatcatt gagctcctcc attttcaacg
      961 tcggtgcggc ggtactccct gtccaggaaa ctaaatctaa attctcagct tgaagcacaa
     1021 tactgtgttg cttataaacg ccatactcaa ccccttcagg ggtcagaatt tcaagataat
     1081 aacgtatctg tgttagatac cccgaatatg ccaccggggt caccagtgtc gcaattttct
     1141 tcgctgccag actttctgct gcgcaagtca gcatgaccag gcagcacaac catcggaata
     1201 ttttcatttt atatgtactc aaatgtcgca gtcagggagg cggtaaaatc ccccatcgtc
     1261 acttcccggc cgcttttcgc ctggagccag gcattaaaat tcagattgtt attgccgtta
     1321 ctcagcaaaa aggtcgcgcc gctggtgttg ttgatcggca ccgccgttcc gtcggttttt
     1381 tccatgccaa tccccacgcc ctgcgcccct gaggacgcat ccagcgccag aaagccgggc
     1441 tgttcactgt cttctgtacc ggtcagcgtc acccggactt catactgagc tggccctttg
     1501 caatctttca gctgaaacac taccggcacc cgcgccgact gcccggtcac catcaggtct
     1561 ttgcggctaa cggtggggaa atgcacctcc gccagcgact cgccctgaac gactaacgtg
     1621 caggacttgc tgagtaaatt gccgtagaaa tgcaggttgt cagccgcctg aacatggggc
     1681 gtcagcagca ccgccgccag tgcagccgcg cagtgagata tccgttttag gttcattggt
     1741 aatccacctg taatgttgcg taggcagaaa aggttccgtc gctgagcgcc gcatcattgg
     1801 ctttcaccgg cacagcttgc agcgttggcg gcgttgacgg gtcaattttc aacggtgttc
     1861 ccagcttaaa cggctggccg ttttgctgta gctcaatccc caggccgcta acgtccgttt
     1921 caatggcgga atcatcataa ggggtttggc tgcccgtcca ggtcaggctc atctcccagg
     1981 cgtcgtcgcg cacatcgggg tcacatgtga tggtgtaagg cacatcctga cggaaattat
     2041 cgccgttaat gttgtcgata atgacgttgc gaaactcaac ttcgatcgtc ttaccatcgc
     2101 tgatagtaca ggcgggcggc gaaaccagcg tgccatgaaa agtaacgtcg ttaatcgcgg
     2161 cctgcgccag gccagcaaaa ccgtaaatta agcaccatgc gagtcgtttc attggtagtc
     2221 caccaccatg gtcatggtcg cattaaactc cgatgccatc agttccacac cgctctgctt
     2281 caccggaacg gcttcgaagt ctggctgtgt attgatgttg aacggcagcc agctactgtt
     2341 acccactgcc accagcgaat ggtcagcggc attctgaata cgaatgccaa aaccgctgat
     2401 tccagcactc agcaccgttt cgccgttgat ggtggtggtg gtggcctgaa gctgcatttg
     2461 caggtcgtcg ctgacactgt ttgagcagtt cagggtg
//
LOCUS       DAWDRC010000590         2488 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15498_length_2488_cov_2.868886, whole genome shotgun sequence.
ACCESSION   DAWDRC010000590 DAWDRC010000000
VERSION     DAWDRC010000590.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15498_length_2488_cov_2.868886"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          597 a          585 c          670 g          636 t
ORIGIN      
        1 tgatgtgatt aaccagttga aggtacagaa cgatcagatc gctgccggac agttgggcgg
       61 aacgccagcg ttaccagggc aacagttgaa cgcctcgatt attgctcaga cgcggttaaa
      121 aaatccggaa gaattcggca aagtgaccct gcgcgtaaac agtgacggct cggtggtgcg
      181 tctgaaagat gtcgcaaggg ttgaacttgg cggtgaaaac tataacgtta tcgctcgcat
      241 caacggaaaa ccggcggcgg gcctggggat taagctggca accggcgcga atgctctcga
      301 taccgcgaaa gccattaagg caaaactggc ggaattacag ccattcttcc cgcagggaat
      361 gaaggttctt tacccttatg acaccacgcc tttcgtccag ctttctatcc aggaagtggt
      421 gaaaacgctg ttcgaagcca ttatgctggt gttcctggtg atgtatctgt tcttgcagaa
      481 catgcgggca acattgatac ccaccattgc tgtacctgtc gtgttgttag ggacatttgc
      541 catactcgcc gcttttggtt actccatcaa tacgctaacg atgtttggga tggtacttgc
      601 catcggactt cttgttgatg atgcgatagt cgttgtggag aacgtcgagc gcgtaatgat
      661 ggaggataaa ctccccccca gagaagcgac ggaaaaatcg atgtcgcaaa tccagggcgc
      721 gctggtaggt atcgcgatgg tactgtcggc ggtatttatt ccgatggcat tcttcggtgg
      781 ctcaacaggg gcaatttatc gccagttctc tatcactatt gtttcagcaa tggcgctttc
      841 agtactggtt gcgctgattc tcacccccgc gttatgtgca acattgctga aaccagtctc
      901 tgccgagcat cacgaaaata agggaggatt cttcggctgg tttaacacca ctttcgatca
      961 tagcgttaac cactacacca acagcgtcgg caaaatcctc ggttccacag ggcgatattt
     1021 actgatctat gcgctgattg tcgccggaat ggtagtgctg tttttacgcc ttccgtcttc
     1081 cttcttacct gaagaggatc agggtgtctt tctgaccatg attcagttac ccgctggcgc
     1141 gacacaagag cggacgcaaa aagtgttgga tcaagtgacg gattactatc tgaagaacga
     1201 gaaagcgaac gttgaaagtg tctttacagt taacggcttt agcttcagcg gccaggcaca
     1261 aaacgccggt atggccttcg tcagtctgaa accgtgggaa gagcgtagtg gtgacgaaaa
     1321 cagtgcggaa gcagtaatcc atcgtgccaa aatggaattg ggcaagatcc gcgacggttt
     1381 tgtcattcca ttcaatatgc cagccattgt tgaactgggc acggcaacgg gtttcgactt
     1441 tgagttaatt gatcaggctg ggctgggtca cgatgccctg acccaggccc gtaaccagtt
     1501 gcttggcatg gcggcgcaac atcctgccag cttagtcagc gtgcgcccta atggcctgga
     1561 agacaccgcg cagtttaaac tggaagttga ccaggaaaag gcgcaggcat taggtgtttc
     1621 actttctgac atcaatcaga ccatttcaac ggcgttgggt gggacttacg ttaacgactt
     1681 catcgaccgt ggtcgcgtga aaaaggtgta tgttcaggcg gatgccaaat tccgtatgct
     1741 gccagaagat gtcgataaac tttatgtccg cagcgccaac ggcgaaatgg tgccattctc
     1801 tgcctttacc acttcacatt gggtgtatgg ctctccgcga ctggaacgct acaacggtct
     1861 gccgtcaatg gagattcagg gggaagccgc gccaggaacc agttccggcg atgccatggc
     1921 gttgatggaa aaccttgcat caaaattacc ggcgggcatt ggttatgact ggacgggtat
     1981 gtcatatcag gaacgtttat cgggaaacca ggctcccgct ctggtagcaa tttcctttgt
     2041 ggttgttttc ctgtgccttg ctgcactcta tgaaagaaat gacgtctact ttatggtggg
     2101 cttgctaacg acaattggct tgtcggccaa aaacgctatt ttgattgttg agttcgctaa
     2161 agatctcatg gagaaagagg gtaaaggtgt tgttgaagcg acactaatgg cagtacgtat
     2221 gcgcctgcgt cctatcctga tgacctctct cgcctttatt ctcggcgtat taccgctggc
     2281 tatcagtaac ggggccggca gtggcgcgca aaacgcagtc ggtatcgggg taatgggcgg
     2341 aatggtctct gcaacgttgc tggcaatctt cttcgtgcca gtgttctttg tggtgatccg
     2401 tcgatgcttt aaaggataaa tcagaaacat aaaggcgctt tcaggcgcct tttttatttt
     2461 cagtgaaccc cataaaaatt aataagat
//
LOCUS       DAWDRC010000591         2486 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15511_length_2486_cov_2.465241, whole genome shotgun sequence.
ACCESSION   DAWDRC010000591 DAWDRC010000000
VERSION     DAWDRC010000591.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15511_length_2486_cov_2.465241"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    637..646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          566 a          630 c          634 g          646 t
ORIGIN      
        1 tatccactgg caccggccgc cgcggctttc tcagccagtt tcgcttccag ggcatcgagg
       61 gtggatgcgc catcggcaga aaccacgccg attttattca tgttctgtgc ctgggatgcc
      121 gttaccggtt ccgccgcgaa cacgccaaat gacagagttg aaagagccat agcagcaaca
      181 acagtattga tagttttcat aattaatctc tcgcaggtga tttttgtata agtgacgttg
      241 tttcgtcgat gtgatgagta tcacgttttt ttacgagaga taaaatcgaa gagaattgac
      301 cgccttgttc aaataaattg attgatatct aacatactga taaaccattt atttgttgta
      361 cttatggact tgttttatac cgcgtggcag tcacgctagc gttaaaggca cggaatgcca
      421 cgcaaaatac aaattatatt cgctatacat cccgccagtt gtagttaacg taactggctg
      481 tctttcgaac ctctgcgatt atatcctgta taagctggtt tttgtaaatc tttctcctgc
      541 tgacaatgaa tgcataagcg cacgccagga atggcttccc gacgggcctg cgggataggg
      601 gcaccgcact cttcacattc atacaggctt tcgccannnn nnnnnntttc accccgagcg
      661 cgggcaatcg catcttcaat tgtactgttg atctgttcgt tgacggcgtc atcattagcc
      721 caaccggatg ccatatcgtc ctccccatat caatacttgt acagttaagt gtagctaata
      781 tagggacgaa ctcgggcagt tcaagcgtca gatctccgac cattcccgca gcagattatg
      841 ataaagattg agcagtgaca ggatctcttc actttcgccg tagcggcttt tcagcgactg
      901 aatattgttg tccagttcaa acagcatggc gcgcttttta tcatcacgga tcatcgactg
      961 gatccacata aatgatgcca ctcgtacgcc gcgggttacg ggtgtcacgc aatgcaggct
     1021 gctggaggga tacaacacca gatcgcctgc cgggagtttt acccgatgtt gcccgaaggt
     1081 gtcattaacg accagttcgc cgccgtcgta gctttctgga tcgcttaaaa acagcgtggc
     1141 agaaaggtca gtgcgcatcc agccgttttg tggatggctg cgtactgcgc catccacatg
     1201 aaaaccatag gtttcattgt tctgatagcg attaaacagc ggcgtggaaa gggtacgcgg
     1261 caaggccgcg gcaaagaaca aagcatgttg gttaaccgcg ttcagcacct cattttgcag
     1321 ggcggcgtac agggcgctgc gggtgtctac ttgttgattg ttcttaactt gcgcaccttg
     1381 tgcgccggtg gtgacgcgcc catccaccca ttcggcttgt tccaggtgtt cgcgaaaacg
     1441 ggcgacgtcc tgtggcgata acacgccggg aatgtggtac atcatacaag ttctccaaaa
     1501 agtggggcct gcgccccaca ccgattttta acagcaggca atggtgctca ggtgccggat
     1561 gcggcgtgaa cgccttatcc ggcctaccaa atgatgtgaa ttcagtgtat tgcacaacct
     1621 tgtaggcctg ataagcgcag cgcatcaggc agtcctgctc tcgccatcaa cattagtgag
     1681 ccttgcgccc cacatctgaa tcagaaatgc atattagctg tgagcaagaa ggttcttggc
     1741 tcgcccgggt gataacggta gccgctctta ttgattgagg cgacgtaatc ggtatcaaac
     1801 aggttgtaga cgtttagctg gaagtcgaga ttgcgattaa cccgataccc cagtttggcg
     1861 tcggcgaccc aataaccttc ggtaaacgct ggcgttccca ccgcgccgtc tgaaccttta
     1921 tgcatactgc cgatataccg tgcgcccgca ccaacagaaa tatcatcggt tgcctgatac
     1981 tggctccata aggtgaaggc gtgttccggg gtatacggca gcgatgagga accatcctgg
     2041 gcaacatctt tgccgttttt gatagttgct ttttgctggg tatagccgcc aatcatctgc
     2101 catgcggggg tgatattccc ggccacggat atctcgtagc cttcgacacg tttcttacca
     2161 tattgcgaat aagtcccgtc atcattttgc tcaacttcat tttcaatatc agtacggaac
     2221 agcgcggcgg taagtagcag gcgtttatcc agaacctgcc atttggtgcc aatctcgctg
     2281 gtgttggctt tttgtggttt aaaatcggtg cggttggcac tgttaccgct gccagactgc
     2341 gcaagggcga agttgttgcc gcccggcggc tgctgggaaa cggcatagtt aatatagaca
     2401 ttgccgtttt ccgtcaggtg atacagcgcc ccggctttcc agttcaccag attgcccgac
     2461 ttggcggtgt cgacggtggt gaccgg
//
LOCUS       DAWDRC010000592         2481 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15545_length_2481_cov_2.326876, whole genome shotgun sequence.
ACCESSION   DAWDRC010000592 DAWDRC010000000
VERSION     DAWDRC010000592.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15545_length_2481_cov_2.326876"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          676 a          631 c          545 g          629 t
ORIGIN      
        1 tcaccagatc catgtgcgac ccaacgggcg actggctcca agtgtgcctg actatcaaca
       61 atccgcagtt cgctaaaacc catcgttttc attgcccgcg ccgctgcccc aatattttct
      121 gctctggcgg gtgcaaccag aataatcgtt atacgcatat tgccactctt cttgaccaaa
      181 taaccgcgaa cagggtgatc attgtcaact tattacgcgt tgcgaattta caaattctta
      241 acgtaagtcg caggaaaagc cctttgctta gcttaaaaaa agctaaacta tttcctgact
      301 gtactaacga ttgaattgtt aaaaaatgct acatatcctt ctgtttactt aggataattt
      361 tataaaaaat aaatctcgat aattggattc accacgttta ttagttgtat gaagcaacta
      421 gttggattat taaaataatg tgacgaaagc tagcatttag atacgatcat ttcatcaaac
      481 tgttaacgtg ctacaattga acttgatata tgtcaacgaa gcgtagtttt attgggtgtc
      541 ctgcccctct tagcctgtta tattgctgtt aaaatggtta ggatgacagc cgtttttgac
      601 actgtcgggt cctgagggaa agtacccacg accaagctaa tgatgttgtt gacgttgatg
      661 gaaagtgcat caagaacgca attacgtact ttagtcatgt tacgccgatc atgttaattt
      721 gcagcatgca tcaggcaggt cagggacttt tgtacttcct gtttcgattt agttggcaat
      781 ttaggtagca aacatgcaga ccccgcacat tcttatcgtt gaagacgagt tggtaacacg
      841 caacacgttg aaaagtattt tcgaagcgga aggctatgat gttttcgaag cgacagatgg
      901 cgcggaaatg catcagatcc tctctgaata tgacatcaac ctggtgatca tggatatcaa
      961 tctgccgggt aaaaacggtc ttctgttagc gcctgaactg cgcgagcagg cgaatgttgc
     1021 gttgatgttc ctgactggcc gtgacaacga agtcgataaa attctcggcc tcgaaatcgg
     1081 tgcagatgac tacatcacca aaccgttcaa cccgcgtgaa ctgactattc gtgcacgcaa
     1141 cctgctgtcc cgtaccatga atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag
     1201 ctacaagttc aatggttggg aactggacat caacagccgt tcgttgatcg gccctgatgg
     1261 cgagcagtac aagctgccgc gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc
     1321 aggcaaaatt cagtcccgtg ctgaactgct gaagaaaatg actggccgtg agctgaaacc
     1381 gcacgaccgt actgtagacg tgacgatccg ccgtattcgt aaacatttcg aatctacgcc
     1441 ggatacgccg gaaatcatcg ccaccattca cggtgaaggt tatcgcttct gcggtgatct
     1501 ggaagattaa tcggctttac caccgtcaaa aaaaacggcg ctttttagcg ccgtttttat
     1561 ttttctacct tatttccaga tacgtaattc atcgtccgtt gtaacttctt tactggcttt
     1621 cattttcggc agtgaaaacg cataccagtc gatattacgg gtcacaaaca tcatgccggc
     1681 cagcgccacc accagtacac tggttcccaa caacagcgcg ctatcggcag agttgagcag
     1741 tccccacatc acaccatcca gcaacaacag cgcgagggta aacaacatgc tattacgcca
     1801 gcctttcaat accgcctgca aataaatacc gttcattaac gccccaatca gactggcgat
     1861 tatccatgcc acggtaaaac cggtatgttc agaaagcgcc agcaagagca aataaaacat
     1921 caccaatgac agccccacca gcaaatattg cattgggtgt aaacgttgcg cggtgagcgt
     1981 ttcaaaaaca aagaatgcca taaaagtcag cgcaatcagc agaatggcgt acttagtcgc
     2041 ccggtccgtt aattggtatt gatcggctgg cgtcgtcact gcgacgctaa acgcggggaa
     2101 gttttcccag ccggtatcat tgcctgaagc aaaacgctca ccgagattat tagcaaacca
     2161 actactttgc cattgcgcct gaaaacctga ctcgctaact tcccgtttgg ctggtagaaa
     2221 atcacctaaa aaactgggat gcggccagtt gctggttaag gtcatttcgc tataacgccc
     2281 gccaggcacc acagaaagat cgccggtacc gcttaaattc agcgccacat tcagcttcag
     2341 gttctgcttg cgccagtccc cttcgggtaa agggatatgc acgccctgcc cgccttgctc
     2401 taacccggtg ccgggttcaa tggtcagcgc cgtaccgtta acttcaggcg ctttcaccac
     2461 accaatacca cgcgcatccc c
//
LOCUS       DAWDRC010000593         2480 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15556_length_2480_cov_2.274227, whole genome shotgun sequence.
ACCESSION   DAWDRC010000593 DAWDRC010000000
VERSION     DAWDRC010000593.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15556_length_2480_cov_2.274227"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1745..1754
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          603 a          630 c          689 g          548 t
ORIGIN      
        1 tgcaaaaact gacgcaactc taaggcttaa ccggtggcag gttcaccggt ttactgattt
       61 ttgaagatga tagccatgca aaaaaacgct gcgcatactt atgccatttc cagcttgttg
      121 gtgctttcac taaccggctg cgcctggata ccctccacgc cgctggtaca gggggcgacc
      181 agtgcacaac cggttcccgg tccgacgccc gtcgccaacg ggtctatttt ccagtctgct
      241 cagccgatta actatggcta tcaaccgctg tttgaagatc gtcgaccacg caatattggc
      301 gatacgctga ctatcgtgtt gcaggagaac gtcagcgcca gcaaaagctc ctctgcgaat
      361 gccagccgtg acggtaaaac taattttggc tttgatactg tgccgcgcta tttgcagggg
      421 ttgtttggta acgctcgtgc cgatgtcgaa gcctccggtg gtaacacgtt caacggaaaa
      481 ggcggggcca atgccagcaa tacctttagc ggtacgttga cggtgacggt tgaccaggta
      541 ctggtcaacg gcaacctgca tgtggtgggt gaaaaacaga tcgccattaa tcagggtacc
      601 gaatttattc gcttctctgg cgtggttaat ccacgcacta tcagcggcag caataccgta
      661 ccatctactc aggtggcgga tgcgcgcatt gaatacgtag gcaatggcta cattaacgaa
      721 gcgcaaaata tgggctggtt gcagcgtttc ttccttaacc tgtcgccaat gtaagtgagg
      781 ctgtggtgat taaatttctc tctgcattaa ttcttttact ggttaccacg gcggctcagg
      841 ctgagcgtat tcgcgatctc accagtgttc agggggtaag gcaaaactca ctgattggct
      901 atggcctggt ggtggggctg gatggcaccg gtgaccagac aactcagacg ccgtttacca
      961 cacaaacgct taataacatg ctctcacagc tgggaattac cgttccgacg ggcactaata
     1021 tgcagctaaa aaacgtcgct gcggtaatgg tgacggcgtc acttccaccg tttggacgcc
     1081 aggggcaaac cattgacgtg gtggtttctt ccatgggaaa tgccaaaagt ctgcgtggcg
     1141 gtacgttatt gatgacaccg cttaagggcg ttgacagtca ggtgtatgcg ctggcacagg
     1201 gcaatattct ggttggcggc gcaggggcct ccgctggcgg tagcagtgtt caggtgaacc
     1261 aactgaacgg tggacggatc accaatggtg cggttattga acgtgaattg cccagccagt
     1321 ttggcgtcgg gaatgccctt aatttgcaac ttaacgacga agatttcagt atggcgcagc
     1381 aaatcgctga caccatcaac cgcgtgcgtg gatatggcag cgccaccgcg ttagatgcgc
     1441 ggactattca ggtgcgcgta ccaagtggca acagttccca ggtccgtttc cttgccgata
     1501 ttcagaatat gcaggttaat gtcaccccgc aggacgctaa agtagtgatt aactcgcgca
     1561 ctggctcggt ggtgatgaat cgcgaagtga ccctcgacag ctgcgcagta gcgcagggga
     1621 atctctcggt tacggtcaat cgtcaggcca atgtcagcca gccagataca ccgtttggtg
     1681 gtggacagac tgtggttact ccacaaacgc agatcgattt acgccagagc ggccgttcgc
     1741 tgcannnnnn nnnncaagtg cggcctggga tgcgcaatca ctcaacgaac taaaggcgaa
     1801 agctggcgaa gatccagcgg caaatatccg tccggtggcc cgccaggtgg aagggatgtt
     1861 cgtgcagatg atgttgaaaa gcatgcgcga cgctttacca aaagatggcc tgttcagcag
     1921 cgagcacact cgcctgtata ccagtatgta tgaccagcag attgcccaac agatgacggc
     1981 gggcaaaggt ctggggcttg cagagatgat ggttaaacag atgacgccag aacaaccatt
     2041 gccagaggag cccacgccag cagcaccgat gaaattcccg ctcgaaactg tggtgcgtta
     2101 tcaaaatcag gcgctttctc agctggtgca aaaggccgta ccacgtaact acgatgattc
     2161 gttgccgggt aacagtaaag cattcctcgc gcaactctca ctgcccgccc aactggcaag
     2221 ccagcaaagc ggtgtgccac atcatttgat cctcgctcag gcggcgcttg aatctggctg
     2281 ggggcaacgg caaatccgcc gcgaaaacgg cgagccgagt tataacctgt ttggcgtcaa
     2341 agcctctggc aactggaaag ggccagtcac tgaaatcacc acgactgaat atgaaaacgg
     2401 cgaagcgaag aaagtaaaag cgaaatttcg ggtctacagc tcgtatctgg aagcattgtc
     2461 ggattacgtt gggctgttaa
//
LOCUS       DAWDRC010000594         2480 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15557_length_2480_cov_2.040000, whole genome shotgun sequence.
ACCESSION   DAWDRC010000594 DAWDRC010000000
VERSION     DAWDRC010000594.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15557_length_2480_cov_2.040000"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1708..1717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          593 a          618 c          706 g          553 t
ORIGIN      
        1 gacggctggt cgccgcctgc gaagtttttc agatccatca caataccggt gatattgcgg
       61 tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc ctgcagacga
      121 tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga actgtcgggt
      181 ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc cgtttctttc
      241 gaatcaccac cactgacctg catccagatc cccacaccaa ccagcacgag aaaaataaag
      301 aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca tttaaaaaat
      361 ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc acacctgtct
      421 caggcgcaat agatgtggtt atcgtaatga cccaaccggc acttgtcagc aggaattgcg
      481 gactgggctg taacaaaatc cactctcgtg ttaattttgt gaataaatat catgacagga
      541 gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc ccgcctggct
      601 gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcggctat gcgtgcgccg
      661 gaccataagt ccatgcaacc gtggcatttc tttgtgattg aaggggaagg gcgcgagcgt
      721 ttcagcgccg tactggagca gggggcgatt gctgccggta gtgataacaa agccatcgac
      781 aaagcgcgta atgcgccgtt ccgcgcaccg ctcatcataa cggtggtggc gaaatgcgaa
      841 gaaaatcata aagtcccgcg ctgggaacag gaaatgtctg cgggatgcgc ggtcatggcg
      901 atgcaaatgg cagcagttgc ccaggggttt ggcggcatct ggcgcagtgg tgcattaact
      961 gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat tgtcggtttt
     1021 ctctacctcg gtacgccgca gttgaaagca tctacgtcga taaacgtccc ggacccgacg
     1081 ccgtttgtga cttatttctg ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc
     1141 aggaattcag acactctcac ttatcacttc atggaatgag ggttaccata cccggattgc
     1201 attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac agccacggcg
     1261 ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac agtgagcagg
     1321 cgaagtttgt tgatccgaat ttgcttgtgg gtaatgaaac ccgcgacgat gcggcggtat
     1381 acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg ccgatcgttg
     1441 ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat atcttcgcaa
     1501 tgggcggcaa accgattatg gcgattgcga tcctcggctg gccgattaac aaactttccc
     1561 cggaaattgc ccgcgaagtg accgaaggtg gacgctatgc atgccgtcag gcgggtattg
     1621 cgctggctgg cggtcactcc atcgatgcgc cagagccgat ttttggtctg gcggtaacgg
     1681 ggatcgtacc taccgagcgg gtgaagannn nnnnnnnagc cggatgcaaa ctgttcctga
     1741 cgaaacctct ggggattggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag
     1801 aacatcaggg actggcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg
     1861 ccaacatcga aggcgtaaaa gcgatgaccg acgttacggg ttttggttta ctggggcatt
     1921 taagcgaaat gtgtcagggc gcaggtgtgc aggcgcgcgt cgactatgac gcgatcccga
     1981 aattgccggg ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta
     2041 actttgccag ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gaactgctgt
     2101 gcgatccgca aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg
     2161 tcaaagctac agccgccgag tttggcattg aactgacggc gattggcgaa ctggtgccag
     2221 cgcgcggcgg tcgtgccatg gttgagatcc gttaattcaa tgcggttgtt tattgccgaa
     2281 aaaccgagtc tggcgcgcgc cattgctgat gtcctgccta aaccgcaccg gaaaggcgat
     2341 ggctttatcg agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag
     2401 caggcgcagc cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg
     2461 attgtcccgg aaaagtggca
//
LOCUS       DAWDRC010000595         2477 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15569_length_2477_cov_2.116846, whole genome shotgun sequence.
ACCESSION   DAWDRC010000595 DAWDRC010000000
VERSION     DAWDRC010000595.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15569_length_2477_cov_2.116846"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    228..237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2069..2078
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          651 a          658 c          562 g          586 t
ORIGIN      
        1 cagctggtgg cgtaaccgga agtaaggtga aaacgggcgt caggctgccg tgatggcaat
       61 atgcgcccga aaccggccgc aggggtaaca gcctgcggcc ttttttgtat ccggcaccca
      121 tgaaaaaagt cttaacgcag aacgctaaga ctttattttt caggcaaaaa agattacttg
      181 ctggtatcca gttcttcgaa acctttcacc agatcatcaa tcgctttnnn nnnnnnnaac
      241 ggttccagtt tagccagcgg cagcgcggat ggaccatcac atttcgcatg ttccggatcc
      301 ggatgcgctt caataaacag ccccgccaga cctaccgcca taccggctct tgccagctca
      361 gccacctgag cacggcgacc gccggaagct gcgccaaatg gatcgcgaca ttgcagtgcg
      421 tgggtaacgt cgaaaatcac cggcgaatta ccagacactt tcttcatgat gctgaagccc
      481 agcatatcaa caaccaggtt gtcatagccg aagttagcac cgcgatcgca aagaatcact
      541 ttttcgttgc cgccttcttt gaatttatca acgatattac ccatctgtcc cgggctgaca
      601 aactgcggtt tcttgacgtt aattaccgca ccggttttcg ccatggcttc aaccaggtca
      661 gtctggcgag caagaaacgc cggcaactga atcacatcca cgacatcagc aacgggctgt
      721 gcctgacttg gttcgtgaac gtcggtgata attttcacgc caaaagtctg cttcaactcc
      781 tggaagattt tcatcccttc ttccaggccc ggtccacgat aagagtggat ggaggagcgg
      841 ttggctttgt caaaagaggc tttgaacacg taagggatac ccagtttctg ggtcacggtt
      901 acgtagtgct cgcaaatgcg catcgccaga tcgcgagatt ccagcacgtt cataccgcca
      961 aacagtacga acggcaggtc atttgctacg ttgatgtcgc caatgctaac cactttttgt
     1021 ttcataggat cgccttaatc ttgagtaaaa tgtcgattaa ttaatgcagc acaatatgtt
     1081 tatgcgcgat gttatttatt tgcgcacgga tcatttcgct gatcgggtct tccggacact
     1141 gttcaacgaa ataacttaaa tcgttcaacg caacgtgttc gcaatccagt tgcgcataaa
     1201 tcaacccgcg atcgcgaatt tcatagggat cttccgggtt gaattgtaat aaagcttcgc
     1261 tggtgcgtaa cgccagctcc atctgatttt cttccatcaa cgaagctttg agtgtatcca
     1321 gcaatttgcg gattacctca atgttatcag cttcatcaag gtcttcataa aacagttccg
     1381 ccgacgggct gatatttccc tttaaccaca catccagcat atgttcgctt aacgattcac
     1441 cgttaaaagg attaatcagc caaatttcgc catccggaca ttcaatgcgc aatatcagct
     1501 gcgtagggaa aatcaccggc agcagcggca aatcgagacg attcgcgacc cataataaaa
     1561 ccgcacctaa tgataccgca ctgccctgtc gattctttaa cacctggtcc agccataatg
     1621 catcggaaag acgataaacg ccgcgtgagg ctttaaaccc ccattcgccg taaaacagcg
     1681 cgattaattt ttctagctgc tcttctaatg gcagaagctg gctaatttct tccttcgcca
     1741 gactaacgag acgctccagt tcgtcgtaaa catcttgcga gggaaaatcg cggcggattg
     1801 cttcgcaagc caggatcatg ccttcgcaca atggcgcttt attaaattcg aaatcagcta
     1861 acgatctcat gacttacccc agtaacggta cttttgtggt ggcgagttta atgatgatgt
     1921 acagcaccac caacgccagc gggaaggcaa taatgcgcgc ctgctgactg tgcatacgac
     1981 gataatcgag tgcaataaaa cccaaaacaa tataaatgat aactccaaac agcttttcag
     2041 tcagccactg tgcctgcccg gaaaatggnn nnnnnnnncg tatcgacaac cggcggcaca
     2101 atgcgcgtcc agcgagcaaa tgcctgtgga tgcttctgat aacgtagcca gaaacgtaag
     2161 gtcaatagcc caacagaaag cgcgatacta atgagatgaa cgctaagcaa cgtagaaaaa
     2221 cttgtcattg ataatagcgg ccgagcgtta cgcgctcgtt atccccatag tcacggcagg
     2281 tttcgacgtc atgatacccc gcgaagataa atgcctgtcg caccgcttca ccctgctgcc
     2341 agccatgctc cagaagcaga aagccgccgg ataccagcgc gttacgcgac tgttcgatga
     2401 tatgcacgat gtctgccatt ccgctgtctg ccgcaaccag cgcagtgagt ggctcaaagc
     2461 ggacatcgcc ttgttga
//
LOCUS       DAWDRC010000596         2477 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15571_length_2477_cov_1.839802, whole genome shotgun sequence.
ACCESSION   DAWDRC010000596 DAWDRC010000000
VERSION     DAWDRC010000596.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15571_length_2477_cov_1.839802"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    360..369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          683 a          585 c          537 g          662 t
ORIGIN      
        1 taaacagcga ctaaacagaa tattattgcg aacaagggaa gtgtcaggaa aggtcaagag
       61 gtactaatag tttatatggc tataaacatt agctatggtt catgtttgcc tggtgaaact
      121 aacaccaaat tgctgtaact ctctttgaat cataaactga aggatgatgc agcacagttc
      181 ctcgtggact atcctggcta ctacatgacc aaacgcggat atcatcctcg agcagtcaac
      241 tctggcaatc tttttactca gctatgagga cctgatcgtc gactttgcca tctgcatgat
      301 gaatctcctc agcagcagat gcattattta ccgaaatgaa tcccatggaa taatgcagtn
      361 nnnnnnnnng taattagtag tgctttcata tctgatacct gttttaaacg atatatttgg
      421 aacacgccga agctcatccc cgacgggaat cggggcgatc ggcgctttac cttcaatgtc
      481 ttcgatgcca cgttgtttac gtgtttcaac gactttcatc agctcattca gtgcattcag
      541 gctgcgggga aatcccgtat aggcatagag ctggatgaga atttccttag cttcgtttat
      601 cgtaagtcct gcatcaagtc cttgattaag cgctgcattc agcttatcca tctgactgct
      661 ggccattgat gcggcaatca acggaatggc ctgctggcga gctgacagcg tatctgaaac
      721 cgtatattca tgattcattt cggtgactcc tttattgatg agcgcagcat ttgcggtaag
      781 cgcaaaacca aaggttagca gtaccgctgc cgttaatatc ttaatgggcg tggtattgtt
      841 catctgccac taactccttc cagttaacgc ttttgccatc caccatgccg gttattgcca
      901 gatgtgtcat cgcactaccg ggggccaccc catgccagtg tttaacgtcc gccggacaag
      961 agataacatc gccagcacgg atcacctgca ccggcttgcc ttcttgttgg gtgagtccaa
     1021 caccagaggt cacaatcagt cgctgacctg ctggatgcgt atgccaggcg gagcgggcac
     1081 caggttcaaa gtctccccgg tgcataatag cgacggcgcg cacaatcgac agacccaaca
     1141 ttattaatat aataacaagc ggaatgatga ccattttaaa aaaagtgaat atccatactt
     1201 tcacatcttt aaaaacggat ttccattcaa tgtctaacag gaatgagcct atcagtagca
     1261 ttgaaaccgg gccagttatt gcgcccagca tttttattgg ttgtgcaatg atgtaaggaa
     1321 tatgcacatt tgaaatatat aaaagcaaag caagaataca ggctacaatc cccggattga
     1381 taacgtttga catatttttc aagcatctcg gttaccttat gaacacgcat ttttatcggg
     1441 agaaaccatg aaattgacac ttttaagtgc aatgctgatg acggcagtcg tcggtattac
     1501 ttcagcaaat gcagcagatt acaaaaaatt actgttaccg ccgatgcagc ttatcagggg
     1561 gctagcggtg gggtgccacg taacgtggat aaacctgcga atcgtattga agatatccac
     1621 ggtatggctg atttcatcag ccagtatcaa cgattcaaat cgattgcaac cattagcatg
     1681 tttaattcag gacttgtacg gcgcaatggt atgcaggatt cacagttgga tactattcaa
     1741 caacgtcttc agcaggcttc agatgcacga gctcaggaag tcgccggtgg cgaagtgctt
     1801 tactccggtg atgccaacct gactgatgaa cagatcgcta aattaccttt tgcgttgtat
     1861 cgccagggct acgagtacta ctggaaaacc cacgcacacc caaacaaagg accttcacag
     1921 taaatttaac ataatataca ttatgcgcac caacataaac caagggagaa tccagtcgtt
     1981 ggcggttcat gattgttctg ctcattaaca acgaccaaaa ccccatatct tacttaatac
     2041 cgtattctcg gcttaaccga ccgtgcaacc tcaacacatt ggtttcaact gccgtcacca
     2101 cgttctccgg aaaatctgtc ggtaatgaag tcttcacgtt atccagtgct gccggaatca
     2161 ttctggcaaa gtcactcagg atttcatgca tctgcacttc cgggaatttc agcacctttg
     2221 ctgtcgccag aaaatgtcgc ggataaattt tatcgattgc cgtttttttg cctttggatg
     2281 cgttaagccc cattgccagt ttgagatcgc tgatgtgtat tcccgtaccg ccaaggaccg
     2341 gaaatgccga aatgatgtca taaaatggtg tgagccgata actgccgcca gcctgaataa
     2401 atacggagaa gttttttgca tggccatccg ttgcgccaat catccactgg cagacctgaa
     2461 agttcataaa ctcattg
//
LOCUS       DAWDRC010000597         2472 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15603_length_2472_cov_2.136947, whole genome shotgun sequence.
ACCESSION   DAWDRC010000597 DAWDRC010000000
VERSION     DAWDRC010000597.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15603_length_2472_cov_2.136947"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          651 a          670 c          635 g          516 t
ORIGIN      
        1 acggcgtcag tacgttaccg ccgtgggatg aaagccgcgt tgagaaccct gacgaacggg
       61 tagtaattca gcataactgg cacgagctac gtctgtttat gtgggattac gttggcattg
      121 tgcgcacaac gaagcgcctg gaacgcgccc tgcggcggat aaccatgctc caacaagaaa
      181 tagacgaata ttacgcccat ttccgcgtct caaataattt gctggagctg cgtaatctgg
      241 tacaggttgc cgagttgatt gttcgctgtg cgatgatgcg caaggagagc cgtggcctgc
      301 atttcacgct ggattatccg gaactgctca cgcattccgg tccatcgatc ctttctgccg
      361 gcaatcatta cataaacaga taaaaagcct gggtcagcgc cgtatacgct tcggaatagt
      421 tctggtctgg cccacgaatg actaagcgat cgctaaagca ttctcccgcc tgcggggaga
      481 atgccagcag cacccgatgc ggcagtcgcg cttcgttttc cgccacatcc gtccgcaaac
      541 gtaaatgcca gcccatactt aatgccagtt cagtaaaacc attaccaatc tgctctggca
      601 gcaccacgca gaaaaatccc tcttcggtaa tgcactccgc cgcacaagtc agcaacgatg
      661 ggtgatcaag cgtagtggta tagcgagcct gttcccgttg aggtgtcgcg cactctactc
      721 cctgctgata gtaaggtggg ttgctgatga ttaaatcgaa gcgtactgtc tgctgtgtga
      781 tccactgcag aatatccgcc gtatggacgt taatccgctc tgcccacggg gactggttga
      841 tattttcctg cgcttgcgcg gcagcttcac tttccagttc aactgcatca atcatcacgc
      901 tgtcatctgt tcgctgcgcc agcattaatg ccaacaaccc gctacccgcg ccgatatcaa
      961 ggcaacgttt taccccagcc acaggtgccc atgcgcccag caaaatgcca tccgttccca
     1021 ctttcatcgc acagcgatcg tgagcaacaa aaaactgttt aaaagtaaat ccattacgac
     1081 gaaacacgga tgtagactgt gacatgaaaa taaaaccttg caggaaaaac ggcgatagca
     1141 ccgggtgaga acaatacccg agaagcgata tccatacaaa cagatgaaga ttgcagccgt
     1201 aacgtctata atcagcgccc cacacagagg tagaacatga ctgtaacgac tttttccgaa
     1261 cttgaactcg acgaaagcct gctggaagcc ctccaggata aaggtttcac tcgcccgacc
     1321 gccattcagg ctgccgccat tccgcctgcg ctcgatggcc gtgatcatgc ccgcgaactg
     1381 gcgaaacata cgcatctgga tattgccacc atcaccggcg gcgtggccta tatgaaccac
     1441 gcagaagtgt tcagcgaaaa ccaggacatt gtggtcgcca cgaccggacg tctgctgcaa
     1501 tacataaaag aagagaactt cgattgccgc gcggttgaaa cgctgatcct cgacgaagca
     1561 gaccgtatgc tggatatggg cttcgcccag gatatcgaac atattgctgg cgaaacgcgc
     1621 tggcgtaaac agaccctgct cttttcggca acgctggaag gcgatgcgat tcaggacttt
     1681 gccgagcgtc tgttggaaga tccggtggaa gtttctgcca atccgtccac ccgtgagcgc
     1741 aaaaaaattc atcagtggta ttaccgcgcc gatgatcttg agcataaaac cgcgttgctg
     1801 gtgcatctgt taaaacagcc ggaagcgacc cgctcaattg tgtttgtgcg taagcgtgag
     1861 cgtgggcatg agctggcaaa ctggctgcgc gaagcaggca tcaacaactg ctatctcgaa
     1921 ggtgagatgg tacaaggtaa gcgtaacgaa gcgattaaac gcttgaccga aggccgcgta
     1981 aacgtactgg ttgctaccga tgttgccgcg cgcggtatcg acattcctga cgtcagccac
     2041 gtctttaact tcgatatgcc gcgcagtggc gatacttatt tgcaccgtat cggacgtacc
     2101 gcgcgcgccg gtcgtaaagg caccgcgatt tcgctggtgg aagcccatga ccatctgctg
     2161 ctgggcaaag ttggtcgcta tattgaagag ccaattaaag ctcgcgtaat tgacgagtta
     2221 cgcccgaaaa cgcgtgcgcc aagcgagaag cagaccggca agccatcgaa gaaagtactg
     2281 gcaaaacgcg ccgagaagaa aaaagctaaa gagaaagaga agccgcgggt gaaaaaacgc
     2341 catcgcgaca ccaaaaatat tggtaagcgc cgtaaaccaa gcggaacggg cgtgccaccg
     2401 caaacgacag aagagtaatc tcaatgccgg gtttaagccc ggcattgttt ttatcccggc
     2461 aaaaaaacac tc
//
LOCUS       DAWDRC010000598         2456 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15704_length_2456_cov_2.082049, whole genome shotgun sequence.
ACCESSION   DAWDRC010000598 DAWDRC010000000
VERSION     DAWDRC010000598.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15704_length_2456_cov_2.082049"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    740..749
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1990..1999
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          590 a          594 c          659 g          593 t
ORIGIN      
        1 atcgaatgat gctcgattta gccaatggta tgctattccg ctcacgtttt gcgcgcaaaa
       61 tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgatgacgct gcgctctatt
      121 tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa gccgaactgg
      181 tgctgaatgc actggtagca attcgttatc tcaaaccgca aatgccgaaa agctggcatt
      241 ttgtatctca tggtgaaatg tggatgccca tgccgggcga tgcggcctac gtttggttga
      301 gcgacactca tgagcaagtg aatttactgg tcgttgaatc tggcgagaat gccgcgctat
      361 gtctactggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg ggcgacgcca
      421 ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc agcctcgaac
      481 aggcagtcta acgcgccagc ttaagcgcag tcttcggtac acaactacag caaagaatag
      541 tgccatcatc gcctattgct gattttttta gcggcgtgac ttcgccttct aaaagctgaa
      601 cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt ccctgatttt
      661 ctaattgctc cagcagcatc tgttggttat ttccacgaaa tgcctgtccc tgccagtcaa
      721 tatctacgtt tgcgtccggn nnnnnnnnna cggaaagatt gcagcgtttt taatggttcg
      781 cctgccggat gtttttgccc tttttctggg ctgacggtgg tgaaaataca gcggctacaa
      841 ggcttaacca catcaaacac gacatcacca atgcgaatca ctttccagct atcttcttcc
      901 caggccgatg caccggaaac caccagattg gggcggaatt gctccatttt tacactggcc
      961 ggacaacgtt gttggagatc acgtaacgag gcctcgttag caagaaggta aggatagcca
     1021 tcagcaaatg acaggggtac agtgttgtgg cgtttcacgc gccgggtcat ttgtggcccc
     1081 acccagcgta attgcacttc gcgggagaaa aatccactta gccatttgtt gatcgcgtct
     1141 ggcgcaattc gcgcggtaaa atgtgtgccc caaacttcgg ttggtgcgtc ttgtgtggcg
     1201 aaatcagcaa aacgaacata tgcactactg ccatctggtg cggtgagatg caagccatca
     1261 tgcacgggtg aaggagtaaa ccgtaccatt agcggaaact ggcgagcggt aataaacgta
     1321 ccgtcaggtt ccgtgatcat aaagatgcga tcgaaggcca gaccactgac atctgccagg
     1381 gtatgtgtaa gaccaatgcc gcgcatcgat ttaacaggat gaataaaaag ccgggttaat
     1441 gtcgccacgg tgcggtcctc aaatgaaaat aagccctcaa ctttatgaca tgacgcgctt
     1501 attagctata atgcgcaaca attttcttag agtaaaagtg acaatatgaa ttctctgttt
     1561 gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa cctgggggcc
     1621 gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg gcttgtttac
     1681 cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg cgagtgtaag
     1741 gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac agagatgttt
     1801 aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat acgcaacagt
     1861 cagtacggtg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg gaaaaatctg
     1921 ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg gctgcataaa
     1981 gaaaccgccn nnnnnnnnng tggttcaggt acgttgctga ttgaagcggc tatgttggcg
     2041 accgaccgcg caccgggctt gcaccgtggc cgttggggct ttagcggctg ggcgcagcat
     2101 gatgaagcta tctggcagga agtgaaagcg gaagcgcaaa ctcgtgcccg taaaggcctg
     2161 gcggagtata gttcccattt ctacggttcg gacagtgacg cacgggtgat tcaacgtgcg
     2221 cgcactaacg cccgtcttgc ggggattggt gaactgatca cctttgaggt gaacgatgtc
     2281 gcgcaactgg ccaatccgct gccgaaaggg ccgtacggta cagtgttgag caacccgcca
     2341 tacggtgaac gtctggacag cgaaccggcg ctgattgcgc tgcatagtct gctgggccga
     2401 atcatgaaaa accagttcgg tggctggaat ctctctttgt ttagtgcctc gccgga
//
LOCUS       DAWDRC010000599         2421 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_15936_length_2421_cov_2.148774, whole genome shotgun sequence.
ACCESSION   DAWDRC010000599 DAWDRC010000000
VERSION     DAWDRC010000599.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15936_length_2421_cov_2.148774"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1663..1672
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2067..2076
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          493 a          658 c          625 g          625 t
ORIGIN      
        1 gttcagaagg atttggcggt acctgaccgc gcgggatcag gtcaaatttg gcaatagagg
       61 tcggtttagc agcggtagta atatcgccct gaccaatcag aatttccgac aggccattga
      121 cgttattggt gcccaacagc tcgtgggtgt agcctttgcg catatcgcag tcgatcaaca
      181 acacccgttt attggtctgg ctgatcaccg ccgccaggtt ggcgcagaca aaggttttac
      241 cgattgacgg gctaactccg gtcatcatca acacattgtt ctgcgcctgc atcatcgcga
      301 agtgcagact ggtacgcaga ctgcggatgg cttcaatcgc cagatcggtt ggattcccca
      361 ccgccagtag ctggctctgt ttatagcgtt taatcccttt gatggttttg acgctatcgc
      421 gcgctttctg ccactccgac agcgggatgc tggcatagac gctgataccg tgttcttcca
      481 gcacctgcgg gctttcaatg ccgcgattaa acaacgagcg cagcagcaca cccacgatag
      541 agagcatcag gccgaggata atcgccccaa ggataatcag ccctttcttc ggttttagca
      601 caccaggctg agtgattgcc gggtcaacaa tgcgcacatc gccgacggtg ctggcttcgg
      661 tgattttcag ctcctgttct ttattcagca gttgcatata gacctgctgc ccagactcga
      721 catcgcgggt cagacggaca atttcctgct gggttttcgg catcgccgtt acgcgaccat
      781 taagtttggc tttttcgtct tccagcgcct gccgtttctc cagcagcgtg cggtacgccg
      841 gatgaacttt ggtgtacagc ttggagattt ccgcctcttt aaaggtcagt tcgttcaact
      901 gcgcgtcgat gttcaccatc gaatcgagca ccgcttttgc ttccagcggc agatcaacag
      961 aatctttatc ctgacggaag gcattcagtt tgttttcggc aacatcaagg cggctacgta
     1021 cttccggtaa ctgttgcgcg aggaaggcga ggcttttcga cgcttccgcc gatttgcgct
     1081 caatattttg ctcctgatag ttacgggcga tgctgttaag aatgtcgcgg atctgttcgc
     1141 gatcttcacc ggtataagtc aagctcagta cgcctgcgtc tttgccgttc tccgttaccg
     1201 tcaggctgtt ttgcagttgg ttgatcatcc ccagcgtgga gtatttggtg acggtaaact
     1261 cactgcccgg gctggcgtga atggcttcaa ccatcagcgt gacgccttct tttttcagca
     1321 tctggcccgc ttgcccacgg gcgctaaagc cgccgtcgct gcttaaggtg tagtttttgt
     1381 catcaagcac attaagcgta aacacctgat ccgccatctc tttcgggcgg ttaaaggtag
     1441 tcacttttac cgtctcgttc tgacgtccca tcaggcgctc ccagcccgca ccgaaaatcg
     1501 ggaacgtgtt tttgctcacc gcaatatcga ggtcgagatc gtccaccgtt ttaccaagca
     1561 ccaggcgcga acgaatcaac tggatttcgg cgtccgatgc aggcggtttg ttggctaatg
     1621 ccgatccgat atcctgcacc agcgaattgc cgctgctttg ttnnnnnnnn nnatttgtac
     1681 cagtgcgtcg gcgctgtata tcggcgtggc aaagaaggtg taaaccacgg cacagagggc
     1741 gaatacggcg gtaatgccaa tcacccacca gcgtgcttca atgatggtgc cgaccaggcg
     1801 accaatatcg atttcatcac tgcccgttac cggagcggca tgttgtttta ctttttctgt
     1861 cattcttata cctgctctgc gttcaatgcc tgcgcccact ggcgggcaga ccgttcaagt
     1921 aatgtgtaca ccgctgtaaa cgtttcccgg cttttgcgat acggatcggg gatttcacat
     1981 tcgttatccc agtgaccaaa cagcatcact ttgccgcgca tctcgggtgc catctcgcat
     2041 aagcgttcga tatggcgctt ttccatnnnn nnnnnntttc catggtcaga atcaggtcgt
     2101 agttgcgaca cagacggcgg ctgatttgcc gtgcgcaatg accttccagg gacagttgat
     2161 gttctgcggc gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc
     2221 cagcggactc cacttttagc tccgggtgat aacgttgcag taagcgttcc gccgtcgggg
     2281 aacggcaaat attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt
     2341 atgaatgtcg ctggctgtat ccgtcatgta acggacaccg ctaattgttg gccgcaacta
     2401 tttgcacagg cggttccagc g
//
LOCUS       DAWDRC010000600         2407 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16032_length_2407_cov_1.831207, whole genome shotgun sequence.
ACCESSION   DAWDRC010000600 DAWDRC010000000
VERSION     DAWDRC010000600.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16032_length_2407_cov_1.831207"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1301..1310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    2029..2038
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          593 a          586 c          641 g          567 t
ORIGIN      
        1 aggcgctgga ttgcgatttt tacgtgtttt ccgggcataa actgtatggc cccaccggga
       61 ttggcattct ttatgtcaaa gaagccttgt tgcaggagat gccgccgtgg gaagggggcg
      121 gttctatgat cgccaccgtc agcctgagtg aaggcactac ctggaccaaa gcaccttggc
      181 ggtttgaagc cggtacaccc aataccgggg gcatcattgg tcttggcgcg gcgctggagt
      241 atgtgtcggc gctggggctt aataacatag ccgagtatga acagaatctg atgcactacg
      301 cgctatcaca gctggaatct gtaccggatc tcactctcta tggcccgcag aacaggcttg
      361 gcgttattgc ttttaatctc ggtaaacacc acgcctatga tgttggcagt tttctcgata
      421 attacggcat tgctgtgcgt accggacatc actgcgcaat gccattaatg gcctattaca
      481 acgtccctgc gatgtgtcgg gcatcgctgg ccatgtataa cacccatgaa gaagtggatc
      541 gtctggtgac cggcctgcaa cgtattcacc gtctgctggg ataacaggga ggcactatgg
      601 ctttattgcc ggataaagaa aaattgctgc gtaatttttt acgctgcgcc aactgggaag
      661 agaaatatct ctacattatt gagttgggtc agcgtctgcc agaattacgt gacgaagaca
      721 aaagcccgca aaatagcatc cagggctgcc agagtcaggt gtggattgtg atgcgtcaaa
      781 atgcgcaggg aattattgaa ttacagggcg acagcgatgc ggccattgta aaagggctta
      841 ttgcggtcgt ctttattctt tacgatcaaa tgacgccgca ggatattgtc aatttcgatg
      901 tgcgcccgtg gtttgaaaaa atggcgctca cccaacatct caccccatca cgctcacaag
      961 gtctggaagc gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc taaactaaag
     1021 ggacagcttt catccggttg gctcacattg ccgggtggtc aatgtttaca ctggcctgca
     1081 caggagtgtt ttggtttcag gtgaacataa gcgccatccg acgtttcagc gtgagtctcc
     1141 ggcaaataca ggaggtttac aatgaaacgc gcgtctttgc ttacactcac gcttatcggc
     1201 gcttttagcg ccatccaggc tgcctgggcg gttgattatc cgctaccacc aaccggcagt
     1261 cgactggttg ggcaaaatca aacgtatacg gtgcaagaag nnnnnnnnnn gcaagaaggg
     1321 gataaaaacc ttcaggccat cgcccgacgt tttgatactg cggcaatgtt gatccttgaa
     1381 gccaataaca ctatcgcccc ggtgccaaaa cccggtacga cgataactat tccttcacaa
     1441 ctattattac ctgatgcgcc gcgtcagggg attattgtaa accttgcaga gctgcgcctt
     1501 tataattatc cgccgggaga aaatatcgtg caggtttatc cgataggtat tggtttgcag
     1561 gggctggaaa cgccggtgat ggaaacgcgt gtggggcaga aaatccctaa cccaacctgg
     1621 acgcctacgg cgggcattcg tcaacgttcg ctggaacgtg gcattaaatt accaccagtc
     1681 gttcctgccg ggcccaataa cccactagga cgttacgcac tgcgcctcgc gcatggtaat
     1741 ggcgaatacc tcattcatgg caccagtgcg ccggacagcg tcggtttgcg cgtcagttcg
     1801 ggctgtattc gcatgaatgc accggatatt aaagctttat tctccagcgt gcggacggga
     1861 acgccggtga aagtgatcaa cgagccggtg aaatattccg tggaacctaa cgggatgcgt
     1921 tatgttgaag tacatcgacc actatcggca gaagaacagc agaacgttca gacaatgcca
     1981 tacacactgc cagcaggctt tacgcaattt aaagacaata aggctgtann nnnnnnnnaa
     2041 attagtcgat aaagcgttgt atcgtcgggc agggtatccg gttgcggtga gcagtggagc
     2101 aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg aaccagagca
     2161 agggaatatg ttacgcgcga cgcagtagcg gtgaatagca ggcaaaaaaa atggcgcaca
     2221 gtgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta gccatgttgt
     2281 ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg gaacgcattg
     2341 cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg tcagaagaca
     2401 gctgatc
//
LOCUS       DAWDRC010000601         2397 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16109_length_2397_cov_2.227156, whole genome shotgun sequence.
ACCESSION   DAWDRC010000601 DAWDRC010000000
VERSION     DAWDRC010000601.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16109_length_2397_cov_2.227156"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          624 a          515 c          538 g          720 t
ORIGIN      
        1 tctatggctt tagtatattc atcaggaata ccaattgtaa aacaaggtgc ggtatcggta
       61 ggtagatttt ctttgttatc atcatccaga acagaaatag aaaactgtcg ttcaggtgtt
      121 gatgtcgtgg ttattaaaat ggttggatta ggaagagaaa catatgttgt atcatttttt
      181 agttttattt ctttgatgtt ttctttgtta attttatcaa tcgtttcaat agagaaaaaa
      241 gaatcatgcc ccattcgcag ttcaatttta ttatcgtttt cgaataaata aagggcatta
      301 ttgttttgaa acagattgaa tatattgtta gcgcaagttt gagttgctag ccaggatgcc
      361 gctggatgac tcaaattaat atgtgtgcta aagactttga cgcgactact tctgtttttc
      421 tctttacttt caattaaatg cctagcgttt ttggctgctt tacaagttcc atgccccaca
      481 cggcaaaaat aatctcctga aaggatactt acaatcagta ttacaccaat gacgacagaa
      541 aaaatagttc cgatgagttt gatgatgaca atccttgtgc agtgttattt tcaccatgga
      601 ttataatgtt atagtgaaat tattgcgcat gtaatttttc tctgattgtt ttaacatcac
      661 atttatgcac ttcttcatct ttgtagcagg taaaagccat atatggatgg ctggtgtccg
      721 gtgcagataa aatgcaactt attcttgata gcttgaaaaa aagagaggtt tttttgagtg
      781 acggttgata gtcggtggca aggaagacgt tttcgtacac tcggcaagaa aaacgaatca
      841 ctctgtcata ctaccagata catcagcagg gtcaggttgc tcagaaataa gcaggatgag
      901 gatcactgaa gaggcatttt ggcgatcaca ggagtcgcat ttgatggtta agttattgat
      961 ttaatgttta tattttgaat ttgataaatg agtaacccca taagtacccc caaatggttt
     1021 tgcgtatggc gtttgggggg attccgggct actttttcga ctctttattc cactgtagca
     1081 aaccagcgat atccttctcg ccttcggctt cttttaagcg ccgttgttgt tcggcaaact
     1141 gaacatactc cgcctgcgct ttttcatcgg ccattttctt gctgatttta ccagcgccct
     1201 gtagaacctc tcggtcgtta aattgcagga actgatccag cttatcctgc cagtcgcgca
     1261 aaaagacttg ctgacgacga cgggcctgat cctcggcaaa gtccagccac atattaacca
     1321 cgcgattaag ttcgctgacc tcatcctggg tgagataatt ttttgccacc gtcacgtcgc
     1381 ccttacgtac ctcttcacct ttataactgg tcaagcccat gtgcggctgg ttggcatctg
     1441 ctcgtttgtg gatgagttcc gcagcggtat gcccggtaca ggcaaaatgc agtttattct
     1501 ggatggtttg aaaaaattgc gtggtttctt taagcgatgg ctgatagtcg gcagctaaag
     1561 caaagatctc ccgcacccgc aaatataccc gacgctcgct ggcgcgaata tcgcggatgc
     1621 gttcaagcat ctcatcaaaa tagtcgggca caaccgatga accgacaggc ggattcttca
     1681 gccgctcatc gtccatcaca aaacctttga tcaggtattc ctgaagcgtc tgggtggccc
     1741 actggcggaa ttgcgtgcct cgaggcgagc gaacgcgata gccgacagcg agaatggctt
     1801 cgaggttgta atggtctatc tctctggaaa cctgacgttt tccttcctgg cgaactatcc
     1861 ggaatttccg gatggttgag ttttgaacaa gttcaccttc ggaaaatata ttgattaaat
     1921 gttcgttaat agtgcgaaca tctttatcgt acagttcagc cattgccgcc tgggataacc
     1981 agattgtgtc tgactcaaag cgacattcaa cacgcactct tccgtcaccg ctggcaaaca
     2041 taataaactc gcctgctggg ggctgggtta agtctttgtc tgacatgtcg cctccgaagg
     2101 agtgttgttg acgtcgggag agttcaggag aggcattttg gcggaagatc acaggagtcg
     2161 aacctgcccg ggaccgctgg cggccccaac tggatttgaa gtccagccgc ctcaccggag
     2221 acgacgatct tccgcgcctc gattgctaca tggaggcggg gcgcattata gctacttcct
     2281 tgagtttcta catcccccag atcagttttt tccgcctaat acacgcagcg tattatcacg
     2341 aaaacattat agttcatata aaactcatat tttacaatga tatatgagcg aaattaa
//
LOCUS       DAWDRC010000602         2396 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16118_length_2396_cov_2.255874, whole genome shotgun sequence.
ACCESSION   DAWDRC010000602 DAWDRC010000000
VERSION     DAWDRC010000602.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16118_length_2396_cov_2.255874"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    882..891
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          536 a          500 c          645 g          705 t
ORIGIN      
        1 ttgttatgtg attatttaac gtgttgtttt aacgatgatg gctaattatt caggtggttt
       61 ttgagggtgt tttttctcct cggtagaaat ttttattttt aatgtgatca cggttgaata
      121 atcacttttt ctctcttgta tggtagtagg cgtaaaatta agtttgaagt gtctatttac
      181 aatacaggag attgcaataa tgaaaattat tgctgtggat gtttttgtta cttgtcccgg
      241 gcgtaatttc gttacgctta aaataaccac tgaaagcgga ctttgtggat taggagatgc
      301 cacgttaaat ggtcgcgaat tgtcggtcgc ttcttatctg aaagatcatc tttgtactca
      361 attaattggc cgcgatgcca gccgtatcga agatatctgg caattctttt ataaaggagc
      421 ttactggcga cgtggtcccg tcacgatgtc ggccatttct gccattgata tggcgttgtg
      481 ggatatcaaa gctaaagcgg caaatatgcc attgtatcaa ctgcttggtg gcgcttctcg
      541 tgaaggcgtg atggtttatt gccacaccac cgggcggacg attgatgaag tccttgaaga
      601 ctatgcaaag caccagcaga tgggctttaa ggcgattcgt gtgcagtgtg gtgtaccggg
      661 tatgcaaacc acctatggtc tggcgaaggg caaaggactg gcgtatgaac cagcaaccaa
      721 aggcctgtgg ccagaagagc agttgtggtc ttcagaaaag tatctcgatt ttacgccgaa
      781 gttatttgaa gcggtgcgta ataagttcgg ttttaacgaa catctgttgc atgacatgca
      841 ccatcgcctg acccccatcg aagcggcgcg ctttgggagg annnnnnnnn nttaccgcct
      901 gttctggatg gaagatccga cgcctgccga gaaccaggaa tgttttcgtc tgattcgcca
      961 gcataccgtg acgccgattg cggtcggtga agtgtttaac agtatctggg actgcaaaca
     1021 gttaatcgaa gagcagctga ttgactatat tcgcacgacg attacccatg caggcggcat
     1081 taccgggatg cgacggattg ccgattttgc atcgctttat caggtacgta ccggttcgca
     1141 tggcccttca gatttatcgc ctgtttgtca tgcggcggcg ctgcattttg acctctgggt
     1201 gccaaacttt ggcgttcagg agtacatggg ctattccgaa caaatgcttg aagtcttccc
     1261 gcacagctgg cgcttcgatg aaggttatat gcacccaggc gatgaaccgg ggctgggtat
     1321 ttctttcgat gagaaactgg cggcgaaata tccgtatgac ccggcgtatc tccccgtcgc
     1381 acgactggaa gatggcacat tgtggaactg gtaatagcgg ttcgcttaat tattttctga
     1441 atattcatca aatcgattaa ttccgctcaa ggagctaaaa atggacgttg atgtgtcaac
     1501 cagtgttgcg ggtaataaac cgcaacggat tcgtcgtatt cagacagtga ccctggtttt
     1561 attatttatg gcggggatcg ttaattttct cgaccgttcg tcattgagcg tggcaggtga
     1621 agcaattcgt ggtgagttag gattatccgc taccgaattt ggtgttttgc tttctgcatt
     1681 ttctctgtct tatggttttt cacaacttcc ttccggtatt ttgctagacc gctttggccc
     1741 acgaatagtg ttaggcgcag gcttaatatt ctggtcatta atgcaggcat taacaggaat
     1801 ggttaattct tttagccact ttattttaat gcgtatcggt ctgggtattg gcgaagcgcc
     1861 atttatgcct gcgggagtaa agtcgatcac cgactggtat gcgcaaaaag aacgcggcac
     1921 ggcgctgggg atttttaact catctaccgt tatcggtcag gctatcgcgc ctcctgctct
     1981 tgtattgatg caactggcat ggggctggcg gacgatgttt gtcatcatcg gcgtagcagg
     2041 gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac cgggcgcagt ttgtcctgac
     2101 cgaggaagaa cggacatatc tctccgcgcc ggttaaaccg cgtccacagt tacaatttag
     2161 cgagtggctg gcgctgttta agcatcggac aacctggggg atgattttag gtttctctgg
     2221 cgtcaattat accggttggc tctacatcgc gtggttaccc ggttatttgc aggccgaaca
     2281 aggtttcagt ttggcgaaaa ccggttgggt ggcggcaatt cctttcctcg cggcggcggt
     2341 cgggatgtgg gttaacggta ttgttgtcga tcggctggcg aaaaaaggct atgacc
//
LOCUS       DAWDRC010000603         2375 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16269_length_2375_cov_2.037069, whole genome shotgun sequence.
ACCESSION   DAWDRC010000603 DAWDRC010000000
VERSION     DAWDRC010000603.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16269_length_2375_cov_2.037069"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1806..1815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          574 a          572 c          590 g          629 t
ORIGIN      
        1 aagagcacgt ttgcgatccc ccacggatgg cgaatacaaa gctgcgcaat gctgtcattg
       61 ctgaacgctt cccagcgggc ctgctgctga aactgctgag aagacaggaa tgtcccctca
      121 gcccacagtg gtcgacaaat tttcatacat cacaccaccg cccgatcagg cttttgcttt
      181 tggcatctgg gaaaccagag aaaggttgac gtccatccct tcgatctgga aatgcggcac
      241 ggcaaagagt ctgacgcgga aaaagcccgg attgtcctct atgtcctcga cgataacttt
      301 cccgtcgcgc agtggatgcg aagcctgaag ttcatcgccc ggatcggtca tttccgtcac
      361 cagcgtgcgg atccaggtgt taagctccag ttccagcacg cggcggtctt tggtggtgcc
      421 gatattctcg cgctgaataa ttttcaggta gtgagcaatg cgggacagca ggaagatata
      481 gggcaaacgg gcgttgatac ggctgttggc ggtggcgtca gcggtatcat acagcgccgg
      541 tttctgggcg gagtttgccg agaaaaagca ggcgtaatcg cggtttttgt aataagagag
      601 tggaataaag ccaaggttgg cgaactcaaa ctcgcgggtt tccgggatca tcacttcgga
      661 cggaattttg acctgattgc cggtgccgag atcgtaaaga tggatcggca gatcggccac
      721 tgcgccgccc gcctgtgggc cacggatctg cacgcaccag ccattattca caaagctctt
      781 caccatattg gcggcaaagg cgaacgaggc gtttgtccac aggtattttt cgtgatccgg
      841 gcctttgact tcttccacat agttaaagct gcgtaccggc accgtgtccg gcccataggg
      901 cagacggccc agcacgcgcg gcatcactaa tcccacatag cggctgtcat ccgtatcgcg
      961 gaacgacttc catttaatat attctgcgcg gtcaaagtag ttgccgatat ctttaatcgc
     1021 ggcgacttct tccatcgaat ttttaaggaa gaatttcggt ccgacagaac cgataaaagg
     1081 catatgggaa gcggcagaca ctctggaaat attacgcagc agggcaatat cctgcgggct
     1141 gttatcaaat tcatagctgg atattaatga ggctacaggt tcgccacccg gcgtatcgta
     1201 ctcctgaatg tatgtatgtc gatataaacc actcggaatg atttccggtg aatcatcaaa
     1261 atcctgacgc agatgctctt tgctgatatc cagcaattca attctgacat ttttgcgaaa
     1321 atcagtgcgc tgtacgacga accatgtacc acgccacagc gactcgactt tctggaattc
     1381 agggtggtgc atgaccgcat caagctgttt actgattttc tgatccagat cgtcgatatg
     1441 aaaatccagc aggctcttgt cgagcttaac cacgtcctcg cctgatttcg ccagacagga
     1501 aagcagaacg tgaatcgccg ccgtcacgcg ctcatcgggt gaggcatcgg ccagcgtttc
     1561 gctgttttgc cataaatcga tttcctgaat ggaagacacc ggggttaaat taattttatc
     1621 gaacagggac tgataaacgc cgccggactc agtggtggtg agtgttgcag tttcagaggt
     1681 ggcatgttct tgttgtacag acatcagcat ttccttttca gtgatccttt cttctccggt
     1741 ttaacctgaa tacgcgttcg ggttaaacgt tctcctgcgg cgcaattttc gccagttcat
     1801 cacgcnnnnn nnnnngagcg tcgggtcttt caggatattt tccagttcac ggcgaaacgt
     1861 tgcgttatcc agcagattgg atttcaggtc acgtagtaaa ttgcgcatag cgagtaatac
     1921 acgaagttgc ggaatatttt ttgctacctg ttcgggttca aaatccttca ggcttttaaa
     1981 ttcaagcgcg atattttgtt cattaccgtt tccgtttagc gtatcttcta ctgttaaatt
     2041 aaccgcaggc gaaaactcag ccatgacgct gttgaagttg tttttattga tatcaacctt
     2101 gtcccgttcg gacagcggac gttgctcttt tccgttactg taatcgccta cggcaagaag
     2161 cttgaggggg agttcaactt tctttttagc attgcctgta tgcagatcaa gcttgatatt
     2221 tacacgagcg gcgggaactt cattctggaa actgtcggcc atatcctttt cctcatcatc
     2281 cactttttct gatggttatt gacataatta tattcagggg attatcggat gatttaaata
     2341 aaaagcaatg atgaggaaaa tatacaaatg aataa
//
LOCUS       DAWDRC010000604         2374 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16274_length_2374_cov_2.661923, whole genome shotgun sequence.
ACCESSION   DAWDRC010000604 DAWDRC010000000
VERSION     DAWDRC010000604.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16274_length_2374_cov_2.661923"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    681..690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1193..1202
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          550 a          598 c          685 g          521 t
ORIGIN      
        1 tttcagaata aattatttta tattcggtat ttattcgcac atcaggcgat aataacgcta
       61 tgtgatagtc atcaggtttc cctgttacgg aaacctgtgc aacagaatct gtgaacctgt
      121 acagaatatt tttggaaaaa acaccagcga acggaagaac atcgcgatgt atcacaagtt
      181 gatggcaatc aaatagacaa catgaatata actgttagta tttaattgat ttcacttatg
      241 agtatgcgtg tttcatttac ggccaggaat gctgatgatg acaacattac tacccgtttt
      301 cactaagcct tccccattgg cgctcaatgc tctgtgcgct ggtcgtattt gccatttcct
      361 tcttatcccg gatggaagaa tccgctgaac atcgttcggt ggcagttgta acacgcgtcg
      421 attgcttaca gggattatgg cggatcgatt atggataaga gtaagcgcca tctggcgtgg
      481 tgggttgtcg gggcactggc agtggctgcc gtcgtggcgt ggtggctgtt gcgcccggcg
      541 ggtgtgccgg aaggctttgc tgtcagcaat gggcgcattg aagcgacgga agtggatatc
      601 gccagcaaaa ttgccgggcg tatcgacacc attctggtga aagaaggcca gtttgttcga
      661 gaaggtgaag tgctggcgaa nnnnnnnnnn gcaggaacag cgactggaag ccatcgcgca
      721 aatcaaagaa gcacaaagtg ccgttgccgc cgcgcaggct ttgctggagc aacgccaaag
      781 cgaaactcgt gccgcacagt cgctggttaa tcaacgccag gcagaactgg actccgtagc
      841 aaaacgtcat acgcgttccc gctcactggc acaacggggg gcaatttctg cgcaacagct
      901 ggatgacgac cgcgccgccg ctgagagcgc ccgagctgcg ctggaatcgg caaaagctca
      961 ggtttctgct tctaaagccg ctatagaggc ggcacgcacc aatatcattc aggcgcaaac
     1021 tcgcgtcgaa gcggcacaag ccactgaacg gcgcattgcc gcagatatcg atgacagcga
     1081 actgaaagcc ccgcgtgacg gacgcgtgca gtatcgggtt gccgagccag gcgaagtgct
     1141 ggcagcaggc ggtcgggtgc tgaatatggt cgatctcagc gacgtctata tgnnnnnnnn
     1201 nnagatctgc gtattcccgc gaccatcagt tttgtcgcca gcgtcgccca gttcacgcca
     1261 aaaactgtcg aaaccagcga tgagcggctg aaattgatgt tccgtgtcaa agcgcgtatc
     1321 ccaccggaat tactccagca gcatctggaa tatgtcaaaa ccggattgcc gggcgtagcg
     1381 tgggtgcgag tgaatgaaga acttccgtgg cctgatgacc tggtggtgag gttgccgcaa
     1441 tgacgcatct ggaactggtt cccgtcccgc ctgtcgcgca actggcgggc gtgagccagc
     1501 attatggaaa aaccgctgcg ctgaacaata tcacactcga tattccggcc cgttgcatgg
     1561 tcgggctgat tggaccggat ggtgtcggta aatcgagctt gttgtcgttg atttccggtg
     1621 cccgcgtcat tgagcagggc ggcgtgatgg tgttgggcgg cgatatgcgc gacccgaagc
     1681 atcgccgcga cgtctgcccg cgcatcgcct ggatgccgca ggggctgggc aaaaatctct
     1741 accacacctt gtcggtgtat gaaaacgtcg attttttcgc ccgcctgttc ggtcacgaca
     1801 aagcggagcg ggaagtgcga attaatgagc tgctgaccag caccggatta gcaccgtttc
     1861 gcgatcgtcc ggcagggaaa ctctccggtg ggatgaagca aaaacttggg ctgtgctgtg
     1921 cgttaatcca cgacccggaa ttgttgattc ttgatgagcc aacaacgggg gttgacccgc
     1981 tctcccgcgc ccagttctgg gatctgatcg acagcattcg ccagcggcag agcaatatga
     2041 gcgtgctggt tgccaccgcc tatatggaag aggcggaacg cttcgactgg ctggtagcga
     2101 tgaatgccgg agaagtgctg gcaaccggta gcgccgaaga actgcggcag caaacgcaaa
     2161 gcgccacgct ggaagaagca tttataaatc tgttaccgca agcacaacgg caggcgcatc
     2221 aggcggtagt gatcccaccg tatcaaccta aaaacgcaga gattgccatc gaagcgcgcg
     2281 atctgaccat gcgttttggt tcctttgtcg ccgttgatca cgtcaatttt cgcattccac
     2341 gcggggagat ttttggtttc ctcggttcga acgg
//
LOCUS       DAWDRC010000605         2358 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16396_length_2358_cov_2.499783, whole genome shotgun sequence.
ACCESSION   DAWDRC010000605 DAWDRC010000000
VERSION     DAWDRC010000605.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16396_length_2358_cov_2.499783"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    743..752
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1442..1451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          524 a          674 c          612 g          528 t
ORIGIN      
        1 aagtggatcg cctgctcctt attactaaac gagtgatcaa caaagatatt ctctagtgcc
       61 agcagtgggc gaacgtcttc ttccggcgca aacgcggtaa gtaacgcttc aatttcctgc
      121 gcgctgcggc actcgcacgc ctgacgcgcc agttcccgac acgcctcgct atccagttga
      181 cgaagctggc ttttcacttc cggaatacgc gggctactca tgcttagctc atccaggccc
      241 agccctagca gtagcggcag ataacgactt tcaccgccca gttcaccgca aatacctacc
      301 catttgccgc gctgatgcgc agcggtaaca atctgctgca acatgcgtag gaacgatggc
      361 gtaatcgggt tatacagcgg cgatacgcgc gggttattac gatcgaccgc atacagatac
      421 tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa gtggtcgatg
      481 atgtagcaca ccgacggcac ttccaccatg atacccagcg taatcgtctc tgcatgacgc
      541 aggccatcgc gcttaagctc aacgatcgct ttttggatct cacccttcac ccataagatc
      601 tgatcgagac tgtggaccat tgggatcatc aactgggcgt tgccgaaact ggcagcgcgc
      661 aaaatcgccc gcaattgagt acggaacagg ccagcaaatt ccgggtaaat acgtaccgcg
      721 cgatagccga ggaacgggtt ctnnnnnnnn nntccatcgt gcggaagata atcggcttgt
      781 cgcccgccgc cagtagcacc tgctggtacg cttcaaactg ttcctgctcg tccggcgcgc
      841 tgtcgcgatc catatagagc atttcggtac gaaacagccc aacgccttcc gcgccgttgg
      901 caaacgcgcc tggggcttcc agagctgtgc caatattcgc cgcaatatca atacgctttt
      961 tatcacggga ataggccagc tgcgcggcag cttgcgcctg ttgtttttgg cgtttatccg
     1021 ccagcgtctg cgccacctgg taataaccgc tcaccgcatc gttgggatta attgccagta
     1081 cgccgcactg ggcatcaagc actgcaggtt gcccggcata acgggcaatc gcatccagcg
     1141 gcaggccgct cagtaccggg atcgccgaag cgcgggccag aatcagggta tgcgaggtgc
     1201 gcccggtttt ctccagaatc atgcccgcaa gattttttaa atcgaggctc aaaaactggc
     1261 ttggggttaa atcttcagcc accagaatgg tcggtttttc aagcaccaga ttattgcgcg
     1321 gttttagttc cggccaggtg atatgcagca actgttcgct aatatcgcga atgtcgctga
     1381 cccgctcgcg cagataatcg ctggcagaag cagaaagttt ggcacaaatc tgctccatat
     1441 tnnnnnnnnn ngttcagttg ctcggcaagc gttgccagac tgtgctccag cagggtggaa
     1501 tcttgcgcac tggccggtat tgcccgataa ctgtcgaggc tgtcgctctg taacagggtc
     1561 agtgtaccca cgccaacgcc gcttgccagc acgttgccgt acagtaaatc cgggttcagg
     1621 cggcttaatg aacgcggcag cggatgcgcc gtcagttctg cctgcgtcgg ctggacgcta
     1681 tcgctgtcga taaagcgcac ctggatatac tcttccagca ctcgccgcgc ctgctcttca
     1741 tcgctaccgc taatgttcag gctgcaactg tcattaaata aagtgccagt gccaatcagc
     1801 gccagcgagc ttttcgcatc ggcctttgcg ttctggcgat ggttaataaa agtgatttcg
     1861 ctttgccact ggctgcactg ctctttaagt tcccacgccg gacgagcatg tagaccgtta
     1921 ggcaaaggac agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat
     1981 ctgaaatccg tcagttgaac aaccgtctca cgcgtaagac gttgaataag ggcagataaa
     2041 agttgctcca tctgcaaaat gtcacggcag tcggcaatcg atgcggcgca atgtccgtgg
     2101 cgggttgcag gccccatcac cactgtgggt acgccagtgc cggttaagtg caccgcccca
     2161 ccgtccgtgc cgccattgct gaacatatct gactgcaacg gtacgccaat ttctgccgcc
     2221 acggtttcga tccaggcggt aagttttggc ggcgcaatca gtgacttgtc gcttaacacc
     2281 agcatcggcc cgttaccaat ctggcgatgg ttagctgcgc cataatcaaa gtttttcgcc
     2341 cagcaggcgg tatcaagc
//
LOCUS       DAWDRC010000606         2353 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16438_length_2353_cov_2.516101, whole genome shotgun sequence.
ACCESSION   DAWDRC010000606 DAWDRC010000000
VERSION     DAWDRC010000606.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16438_length_2353_cov_2.516101"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          631 a          563 c          662 g          497 t
ORIGIN      
        1 ttcaaaactt ctcgcatagg cgaactggct accgtgactc ctggtaccga tcttaccgtc
       61 gttgatgcca gtggcaaaca gccaattgtt cggttgcagg gttatcaaat gcagggcagt
      121 gaaaacacgc tctacctggc ggcaggccaa cggctggcgc tcgccacatt aagtgaagaa
      181 ggtatcaagg cgctaacggt aaacggggaa tggcaggctg acgaatacgg caatcaatgg
      241 cgtcaggcgt ctttgcaggg ggcgcttatc gatcccgcat tagcggaccg taaaccgcta
      301 tggcaatacg ctgaaaaact tgacgatacc tattgcgctg gctgccatgc ccctattgcc
      361 gccgaccatt acaccgtcaa tacatggccg tccattgcca aaggaatggg tgcgcgaacc
      421 agcatgagcg aaaacgaact ggacattttg acgcggtact tccagtacaa cgccaaagat
      481 attaccgaga aacagtgatc ctcgttgatc agggaggaag ttatgacatt aacaagacgt
      541 gaatttatta aacatagcgg tattgcggta gggacgctgg tggtgacatc ggctgcaccg
      601 ctacccgcat gggcagaaga gaagggcggc aaaattctca ctgcgggccg ttggggagcg
      661 atgaacgtag aagtgaagga cggcaagatt gtttcttcga caggcgcgct ggcgaaaacc
      721 ataccgaact ccttacagtc tacggcggcg gatcaggtac acaccacggc gcgtattcag
      781 catccgatgg tgagaaaaag ctatctcgat aatccactgc aaccggtgaa aggtcgtggc
      841 gaagatacct atgtacaggt gagctgggaa caagcattaa aactgattca tgaacaacac
      901 gatcgcattc gcaaagctaa cggaccatcg gccatttttg ccggatctta tggctggcgt
      961 tcttctggtg tgttgcataa agcgcaaacc ttactgcaac gttatatgaa cctggcgggc
     1021 ggttattccg ggcatagcgg cgattattca accggagcgg cacaggtgat catgccgcat
     1081 gtggtcggtt ctgtggaggt ctatgaacag cagacctcct ggccgctgat tctggaaaac
     1141 agccaggtgg tggtgctgtg ggggatgaac ccgctgaaca cgctcaaaat tgcctggagc
     1201 agtaccgatg agcaagggct ggaatacttc catcagctga aaaaatctgg caaaccagtg
     1261 attgccattg atcctatccg ctccgaaacc atcgaattct ttggcgataa tgccacctgg
     1321 atcgcaccga atatgggcac cgatgtggca ctgatgttag ggattgcgca taccctgatg
     1381 acacaaggca aacacgataa agtgttcctt gaaaaataca ctaccgggta tccgcagttt
     1441 gaagagtatc tgacaggtaa gagcgataat acgccgaaga gcgcggcctg ggcggcggaa
     1501 attactggcg taccggaagc gcagatagtc aaactcgctg aactgatggc ggcaaatcgc
     1561 acaatgctga tggcaggctg gggaattcag cgccagcaat atggtgaaca gaaacactgg
     1621 atgctggtga cgctggcggc gatgttaggg cagattggta caccgggcgg cgggtttggt
     1681 ttctcttatc attactccaa cggtggcaac ccgacgcgtg ttggcggcgt gctaccagaa
     1741 atgtctgcgg cgattgccgg acaggccagc gaggccgctg atgatggcgg aatgacggcg
     1801 attcctgttg cccgtatcgt cgatgcgctg gaaaatcctg gcggcaaata tcagcataac
     1861 ggtaaagaac aaacgtatcc gaatatcaaa atgatctggt gggcgggtgg cggtaacttt
     1921 acccatcacc aggatactaa tcgtctgatt aaagcatggc agaaaccgga gatgatcgtc
     1981 gtttctgaat gctactggac cgcagcagcc aaacacgcag atatcgtatt accgatcacc
     2041 acatcgtttg agcgcaatga cctgacgatg accggtgatt acagcaacca gcatattgtg
     2101 ccgatgaagc aggctgtcgc tccgcaattt gaagcgcgca acgattttga cgtgtttgcc
     2161 gatcttgctg aattactcaa acctggcgga aaagagatct ataccgaagg taaagatgaa
     2221 atggcgtggc tgaaattttt ctatgatgcc gctcagaaag gtgcgcgtgc gcaacgcgtg
     2281 acgatgccaa tgtttaatgc cttctggcag caaaataaac tgatcgaaat gcgccgcagc
     2341 gagaagaacg aac
//
LOCUS       DAWDRC010000607         2348 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16469_length_2348_cov_1.779765, whole genome shotgun sequence.
ACCESSION   DAWDRC010000607 DAWDRC010000000
VERSION     DAWDRC010000607.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16469_length_2348_cov_1.779765"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1915..1924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          635 a          528 c          580 g          595 t
ORIGIN      
        1 tgcgccaggg caaatctcgg taaatgattt gcgtacggta ttaactattt tacatcaggc
       61 ataagcaata atattttcgg cgggaatacc ctctcccgcc gaactaaaaa aagtattttt
      121 aaccgtattt aataaaaata tttcgtgagt ctctgtgcgc taattctcca tctggcgtag
      181 ggaaaatcac acctgaatca ggaattaaca atgaaacctg taaaaccacc tcgtattaat
      241 ggacgagtgc cggtcctgtc ggcacaggaa gcggtgaatt atattcccga cgaagcaaca
      301 ctttgtgtgt taggcgctgg cggcggtatt ctggaagcca ccacgttaat tactgctctt
      361 gctgataaat ataaacagac tcaaacacca cgtaatttat cgattattag tccaacaggg
      421 cttggcgatc gcgccgaccg tggtattagt ccgctggcac aagaaggtct ggtgaaatgg
      481 gcattatgcg gtcactgggg acaatcgccg cgtatttctg atctcgcaga acaaaataaa
      541 attattgctt ataactatcc acaaggtgta cttacacaaa ccttacgcgc cgccgcagcc
      601 caccagcctg gtattattag tgatattggc atcggaacat ttgtcgatcc acgccagcaa
      661 ggcggcaaac tgaatgaagt cactaaagaa gatctgatta aactggtcga gtttgataac
      721 aaagaatatc tctattacaa agcgatagcg ccagatatcg ccttcattcg cgctaccacc
      781 tgcgacagcg aaggctacgc cacttttgaa gatgaggtga tgtatctcga cgcattggtt
      841 attgcccagg cggtgcacaa taacggcggt attgtgatga tgcaggtgca gaaaatggtt
      901 aagaaagcca cgctgcatcc taaatctgtc cgtattccgg gttatctggt ggatattgtg
      961 gtggtcgatc cggatcaaac ccaactgtat ggcggtgcgc cggttaaccg ctttatttct
     1021 ggtgacttca ctcttgatga cagcaccaaa cttagcctgc ccctaaacca acgtaaatta
     1081 gttgcacggc gtgcattatt cgaaatgcgc aaaggcgcag tggggaatgt cggcgtcggt
     1141 attgctgacg gcattggcct ggtcgcccgg gaagaaggtt gtgctgatga ctttattctg
     1201 acggtagaaa caggtccgat tggcggtatt acttcacagg ggatcgcctt tggcgcgaac
     1261 gtgaataccc gcgccattct ggatatgacg tcccagtttg atttttatca cggtggtggt
     1321 ctggatgttt gttatttgag ttttgctgaa gtcgaccagc acggtaacgt cggcgtgcat
     1381 aaattcaatg gtaaaatcat gggcaccggt ggatttattg atatcagtgc cacttccaag
     1441 aaaatcgtgt tctgcggcac attaactgcg ggcagtttaa aaacagaaat taccgacggc
     1501 aaattaaata tcgtccagga aggacgggtg aagaaattta ttcgggaact accggaaata
     1561 actttcagcg gaaaaatcgc tctcgagcga gggctggatg ttcgttatat cactgagcgc
     1621 gccgtattca cgttaaaaga agacggcctg catttaatcg aaatcgctcc tggcgtcgat
     1681 ttacaaaaag atattctcga caaaatggat ttcaccccag tgatttcgcc agaactgaaa
     1741 ctgatggacg aaagattatt tatcgatgcg gcgatgggtt ttgtcctgcc tgaagcggct
     1801 cattaacagg agtataatga tggatttttc tttaactgaa gaacaagaac tgctgctggc
     1861 cagtattcgc gaactgatta cgactaactt tccggaagag tatttccgca cctgnnnnnn
     1921 nnnntttcca tgcttggcgt gccggaagaa tttggtggta tccctgcgga ttacgtcacc
     1981 caaatgctgg cgctgatgga agtgtcaaaa tgcggtgctc cggcattttt gattaccaac
     2041 ggtcaatgta ttcacagtat gcgccgtttc ggttctgcag agcagctacg taaaacggca
     2101 gagagcaccc tgaaaacggg tgatcccgcc tatgccctgg cattgacgga gccaggcgca
     2161 ggctcagaca acaacagtgc cactaccact tacacgcgta aaaacggcaa ggtttacatc
     2221 aacggacaga aaacctttat taccggtgcg aaagagtatc catatatgct ggtgttggcg
     2281 cgcgatccgc aaccgaaaga tccgaaaaag gcctttactc tgtggtgggt cgactccagt
     2341 aagcccgg
//
LOCUS       DAWDRC010000608         2334 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16572_length_2334_cov_2.028082, whole genome shotgun sequence.
ACCESSION   DAWDRC010000608 DAWDRC010000000
VERSION     DAWDRC010000608.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16572_length_2334_cov_2.028082"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    228..237
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          645 a          526 c          601 g          552 t
ORIGIN      
        1 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
       61 ggtcactggg acaactacaa agatgcaatg ttcaccacgt cttctgagaa ccgtgaatac
      121 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtcg
      181 tatcgcgatt tgccgctgcg tatggccgag tttggtagct gccaccgnnn nnnnnnncca
      241 tcaggttcgc tgcatggcct gatgcgtgta cgtggtttta cccaggatga cgcgcatatc
      301 ttctgtactg aagaacaaat tcgcgatgaa gttaacggat gtatccgttt agtctatgat
      361 atgtacagca cttttggctt cgagaagatc gtcgtcaaac tctccactcg tcctgaaaaa
      421 cgtattggca gcgacgaaat gtgggatcgt gctgaggcgg acctggcggt tgcgctggaa
      481 gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg ctttctacgg tccgaaaatt
      541 gaatttaccc tgtatgactg cctcgatcgt gcatggcagt gcggtacagt acagctggac
      601 ttctccttgc cgtctcgtct gagcgcctcc tatgtgggcg aagacaacga gcgtaaggta
      661 ccggtaatga ttcaccgcgc aattctgggg tcgatggaac gtttcatcgg tatcctgacc
      721 gaagaattcg ctggtttctt cccgacctgg cttgcgccgg ttcaggttgt tatcatgaat
      781 attaccgatt cacagtctga atacgttaac gaattgacgc aaaaactatc aaatgcgggc
      841 attcgtgtta aagcagactt gagaaatgag aagattggct ttaaaatccg cgagcacact
      901 ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag aggtggaatc aggcaaagtt
      961 gccgttcgca cccgccgtgg taaagacctg ggaagcatgg acgtaaatga agtgatcgag
     1021 aagctgcaac aagagattcg cagccgcagt cttaaacaat tggaggaata aggtattaaa
     1081 ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca atggcgaaat tcgcgcccag
     1141 gaagttcgct taacaggtct ggaaggcgag cagcttggta ttgtgagtct gagagaagct
     1201 ctggagaaag cagaagaagc cggagtagac ttagtcgaga tcagccctaa cgccgagccg
     1261 ccggtttgtc gtataatgga ttacggcaaa ttcctctatg aaaagagcaa gtcttctaag
     1321 gaacagaaga aaaagcaaaa agttatccag gttaaggaaa ttaaattccg tcctggtaca
     1381 gatgaaggcg actatcaggt aaaactccgc agcctgattc gctttctcga agagggtgat
     1441 aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg cgcaccagca aatcggtatg
     1501 gaagtgctta atcgcgtgaa agacgatttg caagaactgg cagtggtcga atccttccca
     1561 acgaagatcg aaggccgcca gatgatcatg gtgctcgctc ctaagaagaa acagtaaggc
     1621 cttcaagtag cactgtctgt ggagcctttg ggcttcacag gcattgttcg cctttgtttc
     1681 gtttattaac aatgcgaagt ggaagttatt aaaatgccaa aaattaagac cgtacgcggt
     1741 gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta agcacaagca cgctaacctg
     1801 cgtcacattc tgaccaaaaa agcgaccaaa cgtaaacgtc acctgcgtcc gaaagccatg
     1861 gtttccaaag gcgatctggg cctggtaatc gcgtgcctgc cgtacgcata agccgttaac
     1921 gtttttaact ttttaattag aatatagata caggagagca catatggctc gcgtaaaacg
     1981 tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa caagctaaag gctactacgg
     2041 tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt atcaaagctg gtcagtatgc
     2101 ttaccgcgac cgtcgtcaac gtaagcgtca gttccgtcaa ctgtggattg cgcgtatcaa
     2161 cgcagcagca cgtcagaacg gtatttctta cagcaaattc atcaatggcc tgaaaaaagc
     2221 ctctgttgaa atcgaccgta agatcctggc tgatatcgca gtattcgaca aagtagcgtt
     2281 caccgctctg gttgaaaaag cgaaagcagc tctggcataa gccagttgaa agag
//
LOCUS       DAWDRC010000609         2327 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16629_length_2327_cov_2.582746, whole genome shotgun sequence.
ACCESSION   DAWDRC010000609 DAWDRC010000000
VERSION     DAWDRC010000609.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16629_length_2327_cov_2.582746"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          477 a          557 c          677 g          616 t
ORIGIN      
        1 tcaatgatcg gggagatgaa aaaatgatgc aaatatttag cggtgcttcg tcaggcgcat
       61 ggtttgaaaa agcgcagcgg ttcggcaaat cgttcatgct gccgattgcg gtattgcccg
      121 cagcggggtt gttgttgggg attggtgggg ctctgtcgaa tcccaatacg ctgacggcct
      181 acccattctt gaatgtcgac tggctgcagg caatcttcac aatcatgacc agcgcaggtt
      241 ctattgtgtt tgccaacctg tcggtactct ttgcggtcgg cgtcgccgtg gggctggcga
      301 aatcggataa aggcactgcc gggcttgcgg cattgctagc gtttctggtg atgaatgcta
      361 ccatcaacgc cttattaacg ctaaatggaa cgttggcgca caagaacccc ggttcagttg
      421 gccaggggat gacgcttggt attcagacac tggaaactgg tgtgtttggt ggggtggtga
      481 ttggtctcgt cacctgcacg ttacatagcc gtttcaataa agtctcacta cctcagtttc
      541 tcggtttttt tagtggttct cgttttgtac ccattatcag ctcccttgcc gcgatagtag
      601 tgggtgcggt tatgacggtg gtgtggccgc atttccagaa actcattttt ggactgggcg
      661 gcctggtgga tgcgacgggc tatctgggga cgctgttgta cggcttcatc ctgcgtatgc
      721 ttggtccact gggtttacac catatctttt atttgccgtt ctggacgacg gcgctgggcg
      781 gcagtgaaat cgtcaacggc cagttggttg aaggtacgca gcggattttc tttgcccaac
      841 ttgccgatcc gaatacgcag cagttctacg cgggtaccgc tcgctttatg tccgggcgct
      901 ttatcaccat gatgtttggc ctgcttggcg catgtctggc aatgtaccat accgcaaagc
      961 cggagaatag aaaggttgtc gccggactac ttttgtcggc agccttgacc tctttcctga
     1021 cgggaatcac tgagcctgtt gagttctcct tcctgtttgt cgcgcccgta ttatacgtaa
     1081 ttcacgcctt cttcgatggt ctggcgttta tggtcgccca tattttacat atcactattg
     1141 ggcaaacctt ttcgggcggg cttatcgatt tccttctctt cggcgtgttg cagggggaat
     1201 ctaaaacaaa ctggatgtat gtccccatag tgggaatacc gtggttcttc ctttactact
     1261 tcactttccg ctatttgatt aaccgctttg gctggctgac gccgggtcgt gagaacgtaa
     1321 ccctggttga atccggccag cctcagagtg aacgcgcagc ggcagtcatt gccgggctgg
     1381 gaggcaaaga gaatctggaa gaggtggact gttgcgccac gcgcctgcgc gtgacggtta
     1441 aagagagcag taaggtagat gaagctgcgc tgaaagtgac cggagcgcgt ggcgtgatta
     1501 ttcgcggcaa cggcgttcag gtgatttatg gccctcatgt caccatcatt aaaaacgaag
     1561 tcgaagagat tttatcgtaa tgagaactgt actggattcc ctgaaaggta aattaatcgt
     1621 ctcctgtcag gctctggaaa atgagcctct acatagtccg tttattatgt cacgtatggc
     1681 gctggcggca gcacaaggtg gtgctgcagg tatccgtgcc aacagcgtgg cggatattag
     1741 tgctatcaaa gagattgtct cccttccgat gatagggatt atcaaacgcg attaccctgg
     1801 cagtgaggtg tttatcacgg ccactctccg ggaagtggat gagttgatga ccgttgagcc
     1861 ggaaattatt gccctggatg ctactgcgcg cccgcgccct ggtggtcaga cgctggaaga
     1921 actggtcacg cagatccgcg ctcgctaccc gtccgtactt ctgatggcag atatagcaac
     1981 agtagaagag gcggtgacag ctgaacgtct gggttttgac tgtgtgggca ctacgctcta
     2041 cggctatacc gcagaaactg ccaggcatgt gctggcggaa aatgactgtg gattcttacg
     2101 tgaggtcctg gcagcggtca gcgttccggt tgtggcggaa gggaacgtcg ataccccgga
     2161 acgtgccgca cgttgcctga cattaggcgc tcataccgtg gtggttgggg gggcaatcac
     2221 tcgtccgcag cagataacga cacgctttat cgcggcgatc gcctcgtaaa gcaccgatgg
     2281 ggtatgatac tttggtgggg ataagcgata attatcgctt atcccca
//
LOCUS       DAWDRC010000610         2326 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16636_length_2326_cov_2.274769, whole genome shotgun sequence.
ACCESSION   DAWDRC010000610 DAWDRC010000000
VERSION     DAWDRC010000610.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16636_length_2326_cov_2.274769"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          561 a          638 c          583 g          544 t
ORIGIN      
        1 cggtgtcgga tgcgacgcta atgcgcctta tccgacctac ggttggtgac cgtaaggccg
       61 gataaagtat ttacgccgca tccagcaatc ccttttactt cccttacctt ttctttcaac
      121 gatcacagat ttcgttttat ttcctttttc tccattgaac tttcagtttc ttttctatag
      181 attttaacca acgaaagaaa tcaccaagtg aaatgccgca accggtacat ggactgaaga
      241 agagatccgc catcagcctc gcgcatggat ccgttcactc accaacattg acgcgctacg
      301 ttccgcgctc aatagcttcc ttgaaccgtt actgcgcaaa gagaatctgc aaatcatcct
      361 gaccggagcc ggaacatcgg catttatcgg tgacatcatc gcgccgtggc tcgccagcca
      421 taccggtaaa aacttcagcg ccgtaccgac caccgatctg gtcaccaatc caatggacta
      481 cctgaaccca gctcatccac tactgttgat ctccttcggt cgatccggca acagcccgga
      541 aagcgtcgcc gccgtggaac tggcaaatca atttgtacca gaatgctatc acctgccgat
      601 cacctgcaac gaagcgggcg ctctttacca aaacgcgatc aacagcgaca acgcgtttgc
      661 cctgctgatg cccgcagaaa cgcacgatcg cggcttcgcg atgaccagca gcattaccac
      721 catgatggcc agctgcctcg cggttttcgc acctgagatg atcaacagcc aaaccttccg
      781 cgacgtggcg gatcgttgcc aggcgatcct gacctcactg ggcgatttca gcgaaggtgt
      841 gtttggttac gcaccgtgga aacggatcgt ttatctcggc agcggtggct tacagggcgc
      901 agcacgcgag tcggcgctga aagtgctgga actgacagcg ggtaaactgg cggccttcta
      961 tgattccccg accggattcc gtcatggacc gaaatcgctg gtcgataacg aaacgctggt
     1021 ggtggtgttt gtctccagcc acccttacac ccgtcagtat gatcttgatc tgctggcaga
     1081 actccgccgc gataaccagg caatgcgcgt aatcgccatc gccgcggaaa gcaacgacgt
     1141 tattaccgcc ggtccacata tcatcctgcc gccgtcccgt cactttatcg acgttgagca
     1201 ggcattttgc ttcctgatgt acgcccagac atttgcactg atgcagtcgc tgcacatggg
     1261 taatacgccg gataccccat cagccagtgg caccgttaac cgcgtggtgc aaggcgtaat
     1321 cattcatccg tggcaggcat aagaggatcg cattatgagc attatctcca ccaaatatct
     1381 gttacaggac gcccaggcca atggctacgc ggtgcctgcc tttaacattc ataacgccga
     1441 gacgatccag gcgatcctcg aagtgtgcag tgaaatgcga tcgccggtga tcctcgccgg
     1501 gacgccgggg acctttaagc atatcgcgct ggaagagatc tacgccctgt gtagcgccta
     1561 ttccatgacc tataacatgc cgctggcact gcatcttgat caccacgaat cgctggatga
     1621 tattcgccgt aaagtccacg caggcgtgcg cagcgcgatg atcgacggca gtcatttccc
     1681 gtttgccgag aacgtgaagc tggtgaaatc ggttgtcgac ttctgccact cccgggattg
     1741 cagcgtggaa gcagaactgg gccgcctcgg cggtgttgaa gatgacatga gcgttgacgc
     1801 cgaaagtgca ttcctgaccg atccacaaga agctaaacgc tttgtcgaac tgactggcgt
     1861 cgacagcctg gcggtagcga ttggtacggc gcacggctta tacagcaaaa cgccgaagat
     1921 tgatttccag cggctggagg aaattcgtga agtggtcgat gttcctctgg tgctgcatgg
     1981 tgccagcgat gtcccggatg aatttgtccg tcgcactatt gaacttggcg taacaaaagt
     2041 gaacgttgcc acagaattaa aaatagcctt cgctggcgcg gttaaagcct ggtttgcgga
     2101 aaatccgcag ggtaatgatc ctcgttatta tatgcgcgtc ggaatggatg cgatgaaaga
     2161 agttgtcaga aataaaatta atgtctgtgg ttcagcgaat cgaatttcag cataatcatt
     2221 agttttttat gatttatccc aatgtacttc ccgattaata tcggggagtg ccttaatggt
     2281 aaaggagata actaaacctt aataaataca tcagtacaat atcgca
//
LOCUS       DAWDRC010000611         2320 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16681_length_2320_cov_1.580132, whole genome shotgun sequence.
ACCESSION   DAWDRC010000611 DAWDRC010000000
VERSION     DAWDRC010000611.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16681_length_2320_cov_1.580132"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    312..331
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          578 a          548 c          610 g          564 t
ORIGIN      
        1 aagttccggc gactcaggtt tccgtgtcgg atgcggtatc tacgtatctg tttaacggcc
       61 aactgctgag ccgcgatgat ggttccatga tgttggtgct gcctcaggag tgtcaggaac
      121 acgccggagt atggggttat ctcaatgaac tccttgccgc tgacaacccg attagcgaac
      181 taaaagtttt tgatttacgt gaaagtatgg caaacggcgg cggcccggcg tgcctgcggt
      241 tgcgcgtggt attgacagaa gaagaacgcc gggcagtgaa tccggcggtg atgatgaacg
      301 atacgctgtt tnnnnnnnnn nnnnnnnnnn nctcaatgac tgggtggatc gttactaccg
      361 cgatcgcctt actgcggcgg atcttgccga cccgcaattg ctgcgcgaag ggcgggaagc
      421 actggatgta ttgagccaat tactgaatct cggttcggtt tacccgttcc agcgcgaggg
      481 agggggaaat ggataatttt cttgctctga ccttaacggg taaaaaatcg gttatcaccg
      541 agcgagaaat caacggcgtt cgctggcgct ggctgggcga tggcgtgctg gaactgacgc
      601 cattaacgcc accgcaaggc gcactggtga tttcagcggg aatacacggt aatgagacgg
      661 cacctgtgga aatgctggat gcgttgcttg gcgcgatatc tcacggagag atcccgttac
      721 gttggcggtt gctggtgatc ctcgggaatc ctcctgcgct gaagcaaggg aaacgttatt
      781 gccatagcga tatgaatcga atgtttggcg gtcgttggca gctatttgct gaaagcgggg
      841 aaacctgtcg ggcgcgcgaa ctggaacagt gcctggaaga tttttatgac cagggcaaag
      901 aatctgtgcg ctggcacctt gatccccata ccgcaattcg tggctccttg catccgcagt
      961 tcggtgtatt accgcaacgc gacattccct gggacgagaa atttctgacg tggctgggtg
     1021 cggcggggct ggaggcgctg gtgttccatc aggagcctgg tggtacgttt acccatttca
     1081 gcgccagaca ttttggcgcg ctggcctgta cgctggaact tggcaaagcg ttgccctttg
     1141 ggcaaaacga tcttcgccag tttgcagtaa ctgccagcgc aattgctgcg ctgctatctg
     1201 gtgagagtgt cggtatcgtg agaacaccgc cgctccgtta tcgggtggtt tcgcaaatta
     1261 ctcgccactc gccgtccttc gaaatgcata tggcaaatga cacgctgaat tttatgccgt
     1321 ttgagaaagg aacattgctg gcgcaggacg gggaggaacg ttttaccgta acccatgatg
     1381 tagagtatgt gttattccct aatccgttgg tagcgttggg attacgtgcg ggattaatgc
     1441 tcgaaaaaat aagctaatac ttacccgcag aaatcattct gcgggtatta agttgctttc
     1501 ttataaatta atacagatta atcctggtta tttgctttat ttatcaccag tcatccggta
     1561 tagttcttca taatctctgc aaaatcatcg tgttgtaata ttctctcatc actctccatc
     1621 aaattttctt tttttctcca taattggcgc aaaagtgttt tttacacttt cattgtttta
     1681 ccgttgctct gattaattga cgctaaagtc agtaaagtta atctcgtcaa cacggcacgc
     1741 tacttaagaa agccgtaata aataactgaa aggaaggata tagaatatgc gtaaattaac
     1801 tgcactgttt gttgcctcta ccctggctct tggcgcggct aacctggccc atgccgcaga
     1861 caccactacc gcagcaccgg ctgacgcgaa gccgatgatg caccataaag gcaagttcgg
     1921 tccgcatgag gacatgatgt tcaaagacct gaacctgacc gacgcgcaga aacagcagat
     1981 ccgcgaaatc atgaaaggcc agcgtgacca gatgaaacgt ccgccgctgg aagaacgccg
     2041 cgcaatgcat gacatcattg ccagcgatac cttcgataaa gcaaaagctg aagcgcagat
     2101 cgcaaaaatg gaagaacagc gcaaagctaa catgctggcg cacatggaaa cccagaacaa
     2161 gatttacaac atcctgacgc cggaacagaa aaagcaattt aatgctaatt ttgagaagcg
     2221 tctgacagaa cgtccagcgg caaaaggtaa aatgcctgca actgctgaat aatctttcag
     2281 ccaaaaaacg taagaccgcc ggtcttgtcc actaccttgc
//
LOCUS       DAWDRC010000612         2318 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16691_length_2318_cov_2.176315, whole genome shotgun sequence.
ACCESSION   DAWDRC010000612 DAWDRC010000000
VERSION     DAWDRC010000612.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16691_length_2318_cov_2.176315"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          650 a          572 c          551 g          545 t
ORIGIN      
        1 ttaagcagag aataacgttc gtcgggtgac tgttaacggg ttaacggctt gctggccatc
       61 aacatcaggc tgatagcgat aaccacggcg gccatcatga ccagtaaatg gtcgaccatc
      121 ccgacggcgg taatcaccga gtcgagtgag aagatggcgt ccagtaccac aatttgtgtc
      181 accacccccc agaacttcgc acctttgcgt tgcgtggggt tattgctgtc tttcccttcc
      241 agccgttcgt tcagctccat cgtggctttg aacaacagga agaaaccacc aaacagcatg
      301 attaagtcgc gggcgctaaa ggtaaacgag cggaagctga acagcggttg agtcagggtg
      361 accagccagg agattgacgc cagcagtaac aggcgcatta acatcgccag caggagcccg
      421 gtaacccgtg cgcggtcgcg ctgcttaggc ggtagttttt cggcgaggat ggcaataaag
      481 accaggttat caatgccgag gaccagttcg atcacaatca gcgtgattaa cccagcccag
      541 atagacggat cggcaatcca ttccatagta agtacaaaac ctattcgttg tatgacaaat
      601 gtcacaattc gatcatggat aaatcggggc aaaaatgcaa tgttgcagca ctatttatct
      661 catattattt gagggttata acctcacatt atccccgaat tataagtgat aataataaag
      721 cgttatttaa gaaatatcgc atgtggagac gccatattaa atattgtcaa cctaaagaaa
      781 ctcctaaaaa ccatattgaa tgacacttaa tataattctt aaaaatagcc aattgccgaa
      841 ttgttacctt gcctgctatt ccgttagctg taacacttcc tcctgcatta ttggagagcc
      901 aatattcaat taccataatg tcaccttata ttcggaaatt aatttccgca ataacccttt
      961 ctcatcaacg actgtatgca gagagggttt gaaaaattaa actaaatcag tgtattggta
     1021 gctaaaaagc caggggcggt agcgtgtctg gatgcctgaa agaccagtct gaattattct
     1081 gtcaatagcc tgcggatgaa atcaattttt ttaggactga tgccagttaa atttttattc
     1141 aattaactgc aaggtaaatg tgcatggcga cattgctatt aatagtgcac aggataatta
     1201 ctctgccaaa gtgataaata aacaatgatg aaatccaaaa tgaaattgat gccattattg
     1261 gtgtcagtaa ccttgataag cggttgcaca gtacttccgg gcagcaatat gtcgacgatg
     1321 gggaaagacg tcatcaaaca gcaggacgct gatttcgacc tcgacaaaat ggtgaatgtt
     1381 tatccgttaa ccccgcgcct gattgaacaa ttatgcccac gacccaacgt agcgcgcccc
     1441 aatatgacgc tggaaagtga gatcgcgaat taccagtatc gcgtcgggcc gggggacgtt
     1501 cttaatgtca ccgtctggga tcacccggaa ctcaccacgc cagctggtca gtaccgcagc
     1561 tccagcgaca ccggcaactg ggtacagcca gacggcacca tgttctaccc gtatatcggc
     1621 aaggttcacg tcgtggggaa aaccctcgct gaaatccgca gtgatattac cgggcgctta
     1681 gcgacgtaca tcgctgaccc gcaggtggac gttaatatcg ccgccttccg ctcgcaaaaa
     1741 gcctatatct ccggtcaggt gaataaatcc ggtcaacagg cgattaccaa cgtgccactg
     1801 accattctcg acgccatcaa cgccgcaggt ggcctgaccg acaccgctga ctggcgcaac
     1861 gtggtgctaa cacacaatgg tcgtgaagag cgcatttctt tgcaggcgct gatgcaaaac
     1921 ggcgacctca atcagaaccg actgctttac cccggcgata ttctttacgt gccacgtaat
     1981 gatgatctga aagtgtttgt gatgggtgaa gtgaagaaac agagcaccct gaaaatggac
     2041 tttagcggca tgaccctgac tgaagccctg ggcaatgctg aaggcatcga catgaccacc
     2101 tccaacgcca gcggcatctt tgtcattcgt ccgctgaaag gcgagagcgg gcgtaacggc
     2161 aagattgcca atatctacca gctggatatg tccgatgcga cgtcgctggt aatggcgaca
     2221 gaattccgcc tgcaacctta tgacgtggtg tatgtcacca ccgccccggt atcccgctgg
     2281 aaccgtctga tcaatcagtt gctgccaact attagcgg
//
LOCUS       DAWDRC010000613         2318 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16690_length_2318_cov_2.219178, whole genome shotgun sequence.
ACCESSION   DAWDRC010000613 DAWDRC010000000
VERSION     DAWDRC010000613.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16690_length_2318_cov_2.219178"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    501..510
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1686..1695
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          560 a          560 c          617 g          561 t
ORIGIN      
        1 tccttaaata taaagccttt ctgattgagc aacagtgcgg atattatggc atttttcgct
       61 tatctgcccg tgtgtaattt atgaaaatga ttgaagttgt tgccgccatc atcgaacgtg
      121 atggcaaaat tttactcgcg caacgccctg cccatagcga tcaggcggga ttatgggagt
      181 ttgccggtgg taaagtcgag ccggatgaaa gccagcggca ggcgctggtg cgtgagttaa
      241 acgaagaact gggcatcgaa gcaactgttg gtgactatgt tgccagccat cagcgagaag
      301 tttcggggcg gattatccat cttcatgcct ggcacgtacc cgacttccac gggacgttac
      361 aggcacatga acatcaggcg ctggtctggt gctcacctga agaggcgctg cgatatccgc
      421 tggcccctgc tgacattcca ttattagagg cgtttatggc ttcacgcgcc gccagaccag
      481 cggattagtg cttagggttt nnnnnnnnnn gagtacaggg ctgcgctgac tgcccgcctg
      541 aactgaacac ttctggcggt acgtttacct ccacgtccgg acgatcatgc gggttagcca
      601 gtgcaattaa tggaaatgct aatactacgg cgaacaatgc tcgactcaca gggaactcct
      661 taacgttatt gtctcttcta cagataacgg tagccgggcg gcaaaacttt agcgtctgag
      721 ttatcgcatt tggttatgag attactctcg ttattaattt gctttcctgg gtcatttttt
      781 tcttgcttac cgtcacattc ttgatggtat agtcgaaaac tgcaaaagca catgacataa
      841 acaacataag cataatcgta ttaatatata agggttttat atctatggat cagacatatt
      901 ctctggagtc attcctcaac catgtccaaa agcgcgaccc gaatcaaacc gagttcgcgc
      961 aagccgttcg tgaagtaatg accacactct ggccttttct tgaacaaaat ccaaaatatc
     1021 gccagatgtc attactggag cgtctggttg aaccggagcg cgtgatccag tttcgcgtgg
     1081 tatgggttga cgatcgcaac caggtacagg tcaaccgtgc atggcgtgtg cagttcagct
     1141 ctgccatcgg cccgtacaaa ggcggtatgc gcttccaccc ttcagtaaac ctttccattc
     1201 tcaaattcct cggcttcgag caaaccttta aaaatgccct gacaaccctg ccgatgggcg
     1261 gtggtaaagg cggcagcgac tttgatccga aagggaaaag cgaaggcgaa gttatgcgtt
     1321 tctgccaggc gctgatgact gaactgtatc gccacctggg cgcggatact gacgttccgg
     1381 caggtgatat cggcgttggt ggtcgtgaag tcggctttat ggcggggatg atgaaaaagc
     1441 tctccaacaa taccgcctgc gtcttcaccg gtaagggcct ttcatttggc ggcagtctta
     1501 ttcgcccgga agctaccggc tacggtctgg tttatttcac agaagcaatg ctaaaacgcc
     1561 acggtatggg ttttgaagga atgcgcgttt ccgtttctgg ctccggcaac gtcgcccagt
     1621 acgctatcga aaaagcgatg gaatttggtg ctcgagtgat cactgcgtca gactccagcg
     1681 gcactnnnnn nnnnngtagt tgatgaaagc ggattcacga aagaaaaact ggcgcgtctt
     1741 atcgaaatca aagccagccg cgatggtcga gtggcagatt acgccaaaga atttggtctg
     1801 gtctatctcg aaggccaaca gccgtggtct gtaccggttg atatcgccct gccttgcgcc
     1861 acccagaacg aactggatgt tgacgccgcg catcagctta tcgccaatgg cgttaaagcc
     1921 gtcgccgaag gggcaaatat gccgaccacc atcgaagcga ctgaactgtt ccagcaggca
     1981 ggcgtactgt ttgcaccggg taaagcggct aatgctggcg gtgtagcaac atcgggcctg
     2041 gaaatggcgc aaaacgctgc gcgcctgggc tggaaagccg agaaagttga cgcacgtttg
     2101 catcacatca tgctggatat ccaccatgcc tgtgttgagc atggtggtga aggtgagcaa
     2161 accaactacg tgcagggcgc gaacattgcc ggttttgtga aggttgcgga tgcgatgtta
     2221 agccagggcg tgatttaaga caattgtgcc ggatacagcg caaactgaac tgcaccccaa
     2281 atgttggaca taatctgaca tctgaaggtg cagtttat
//
LOCUS       DAWDRC010000614         2317 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16697_length_2317_cov_2.580018, whole genome shotgun sequence.
ACCESSION   DAWDRC010000614 DAWDRC010000000
VERSION     DAWDRC010000614.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16697_length_2317_cov_2.580018"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          559 a          594 c          579 g          585 t
ORIGIN      
        1 atgtgcggtc gacttcgggt aaagcgccat caagacgctc ttcgtacaag atgctcatga
       61 gtagggttcg ctttgttgtg ttgtgtggca gctgattttt tcgcgctgct tctgtgaaca
      121 gttattaagc gggcttttcg ttttcgtcta tctctttagc taccggtcag accatttttt
      181 ttccagctct gtgaccttgc tttggttaac ttaatgttaa attgatgtaa cataatcact
      241 tacgtgatgt gcgtgttttg cgagttaaga acagaaaaat tggtcctacc tgtgcacgag
      301 gtccgggaat gaaagatgaa cgtcgcccta tttgcgaagt ggttgcagag agtatcgaac
      361 ggttaattat cgacggcgtg ttgaaggtcg gtcagccgct tccctcggaa cgtcggctgt
      421 gtgaaaagct cggcttctca cgctccgcac tgcgtgaagg gctgaccgtg ctgcgcgggc
      481 gcgggattat tgaaacggcg cagggtcgcg attctcgtgt cgcacggctt aatcgggtgc
      541 aggacgccag cccgctgatc catctgttca gtacgcagcc gcgaacgctg tacgatctgc
      601 tcgacgttcg cgcattactg gagggcgaat cggcaaggct ggcggcaacg ctgggaaccc
      661 aggctgattt tgttgtgata acccgctgtt atgaaaaaat gctcgccgcc agtgagaaca
      721 acaaagagat ttcgctgatc gaacatgcgc agttggatca cgctttccat ctcgccattt
      781 gtcaggcttc tcacaatcag gtgctggtgt ttacgctgca atcattgacc gacctgatgt
      841 ttaattcggt gtttgccagc gtaaataatc tctaccatcg accacagcaa aaaaagcaga
      901 tcgatcgcca gcatgcgcgg atctacaacg cggtgttgca gcggctgccg cacgttgccc
      961 agcgcgcagc acgcgatcat gtgcggaccg tgaaaaagaa tctccacgat atcgagctgg
     1021 aaggccacca tttgattcgc tcggcggtgc cgctggagat gaagcagggg tgactgccat
     1081 caaatctgct tttcatacaa tcggtaacgc ttgtacggct ccgccccaat gcgttccagc
     1141 atgttattca tgcctgtatt ggtttcgagg atccatgaca tttccagcgc atcgatcttc
     1201 cggcgggcaa acggatcgtg taaggcttca atcaataaca gcgcaatcac cgggccgatg
     1261 cggctgaact gatactcgtc ccgcacgccc atcagcggca ctcgcgcagt tcgcacaccg
     1321 ctgactttca aacgccacag caattttgcc cagccgaagg gaaagagcga tccgttcaga
     1381 tcgacaatcg cctcgttgat gttcggcaag ccgacaataa acgcgcaggg tgcagaatca
     1441 atctcagcga tgtagatcat atcgtccggc accagatatt taagttgatc gcccatggtc
     1501 gcgaattcat gttcggtaaa cggcacaaat ccccagttgt gctgccagcc agaattgaaa
     1561 atctcacgca ggatctgcat ctcttcggca aaccgctggc gattgatgca gcgaatggtt
     1621 acttttttgc gcacctgatc catcagtttt tttagcgccg gagagaaagt gagatcggtt
     1681 cgctgcatcc accacgccag taaatcaatg cctttgtgat aacccagttg ttcaatatgc
     1741 gcggcatacc acggtttgcc gtgtggcatc atcgcacagg gaggtgtgtc aaaaccttca
     1801 atcagtaatc cgctttcctg attgatattc aggctgaaag gcccgctgat cttacttgca
     1861 ccttgtgact tcagccacgc ctccgctgcg ccaaacaacg cggcaaaaac ctgcggatca
     1921 tcaatggcat caatcatgcc gaaatgaccg gtatcttcac cgtaacgctc gcggtgcaag
     1981 gtatctattt gcgcggtaat ccgcccgact atctgcccgg ctttttttgc tacccacgcc
     2041 tgccagatga tatggtccgt ccccggattc ttcgcagaca aatgctcgct gcgttcaatg
     2101 aataaagggg gtatccagtt tggatcatcg ggataaagtg atgacggaaa agcgataaat
     2161 gccttaaggt cttttttatt aattactttt tcaattttaa tcatcagcag atcgcattcc
     2221 atatctctgt ggataacaaa taattaacgg tgaaaaacag gttgcaacag caacgaacgg
     2281 catcttaata atcaacccaa tgaatacaaa caaagaa
//
LOCUS       DAWDRC010000615         2316 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16706_length_2316_cov_2.371075, whole genome shotgun sequence.
ACCESSION   DAWDRC010000615 DAWDRC010000000
VERSION     DAWDRC010000615.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16706_length_2316_cov_2.371075"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1724..1733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          565 a          596 c          553 g          592 t
ORIGIN      
        1 gtactgacgg ttctgcgccc ggtgcgcaac atctgatcgg tgctcgatgg cgtagcgcgg
       61 ttaacgttta ccgagaagct gaataatccg tcggaataga gacgtgattc gataggcatg
      121 ttgtccatcg tcggtagcgg acgtcgacta ctggaaactt cgctaaaacc ctgtggcagc
      181 caggttggcg tccagctgaa tttagctttt tcaccgacag gaacagaaag caacggcggc
      241 aaatttgcct tcgccagcgt ctgcatactg ctgccgatat cctgattgac gttaaaagca
      301 atcacgcgaa attgttccag cgtttcacca tcgcgatcaa ggagatcaac cctcatcggt
      361 aatttcgatt cggtgtccat ccacacgatg tagctgtagc gtgtaccatc tcgggcaacc
      421 acgcgaatga cttcgcaaag acgatcagca atacgcgtgc gcccgacgga gataaagtcg
      481 tagtaaggag aaaggcgttt gaaatcggta tagatgagcg atggcagaga atcaacaatg
      541 taatcgccat taagcgtgaa cggttcaagt cccggttcaa aatagctgat ttcattgccg
      601 cgctgtacca cttcccggcg cgggccatcc atttgcaaca attgtgcaag aggacggtta
      661 tcgaggcgtg catgtcgata acgcagagac tcaacaccct gtttattgat gctgatgaat
      721 gacagctcgt aattcagtga ctgactggcc aggttcatct gctgtaataa cgccccggac
      781 gcgggagtgg ccgaggcgtt agcagagaat aacaggctac ctgtcactaa tgacatggca
      841 aaccaaagtt gcttcattac tgcgattgcg ttcctaaagt ttgaattcct ggcacctgta
      901 cagcggcttg ctgagtttgt gcctgctcaa actgaagctg ttcagagtgg agtcggcgtt
      961 gcagttcgta atcctgcaac attgcattaa tgcgacgacg ctgctcctgt acctgctgct
     1021 gttgaccatt atttgcggtc gcttcagaag gtactcccag actaaccggg ctggctttac
     1081 ccatcatcgg cagtgtatta aatactggcg tttcgggctg ctgggacgtt tcagattgcc
     1141 cattatagtg ctggacgcca acgataactg caagcgatac gcatgctgct acgcccattt
     1201 gggtaagctg tgccgcccac ggacgtactt tctgccagaa tggcattttc tgccattgat
     1261 gcggcgcagg ctgggcttcc gggatcaatg tcgccggttg acgtactggc tcttcttcaa
     1321 tggcggccat cacgcgtgaa gagatatcga aatggagcac ctcgggagta tcaccccgca
     1381 ttgagtcacg gatcaagtga tagctttccc aggttttctg catttctggg ttatgagcca
     1441 gttcgttaag cagctcacta tccagcgttt cgccatccat taaagcggaa agttgttctt
     1501 tctgcatgcc taataccctt atccagtatc ccgctatcgt caacgcctga taagcggttg
     1561 aactttgtta tcaatagctt ccctcgctcg gaagatacgt gaacgcaccg tacctaccgg
     1621 acaatccatg atagcagcta tctcttcata gctcaggcca tccagctccc gcaaggttat
     1681 tgccatgcgt aaatcttccg ggagggactc aatagttcgg aaannnnnnn nnntgtctca
     1741 gttcttctga caacattaag ttctcagggt tcgaaatttc tttcaacgcg ccgccacttt
     1801 cgaagttttc agcttcaatg gcatccacat cactggaagg tggacgacgc ccctgagcaa
     1861 ccaggtaatt tttcgctgta tttacagcaa tccgatacag ccatgtataa aaagcgctat
     1921 ctccccggaa cgaatccagc gcacgatagg ctttaataaa agcttcttgt accacatcgg
     1981 gaacatcacc cgacggcaca tagcgggaaa ccagactcgc cactttatgc tgatagcgca
     2041 ctaccagtaa gttaaaggct ttctgatctc ccttctggac ccgttcaacc aggacctggt
     2101 ccgttaactg ctcgctcatc cgaggtaaag tctccccaaa ccaaatttcc acgcgctatc
     2161 gaaacgccac tccattagct gcaatttgag caagcaaagg gttagagtgt ctcgtttttg
     2221 taaagttccg taacgcatct gtttttgttt gtcatgctgt agacggatca ttatctatca
     2281 ttataagtct acagaatctg aacatcgcat tatctg
//
LOCUS       DAWDRC010000616         2314 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16719_length_2314_cov_2.204958, whole genome shotgun sequence.
ACCESSION   DAWDRC010000616 DAWDRC010000000
VERSION     DAWDRC010000616.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16719_length_2314_cov_2.204958"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1601..1610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          648 a          535 c          541 g          580 t
ORIGIN      
        1 ccccgcggcg aagagtgctg ttaataacgc ctatgatgca ttaattatcg aagctcgcaa
       61 gggaaatact cagccagctt tgttatggtt tgcacaaaaa tcagcactca gtaataacca
      121 aattgctgac tggttacaga ttgccttatg ggccgggcaa gataaacagg ttattaccgt
      181 ttacaaccgc taccgtcatc agcaattacc agcgcgtggt tatgcagctg tcgccgtcgc
      241 ttatcgtaac ctgcaacaat ggcaaaactc gcttacactg tggcaaaagg cactctctct
      301 ggagtcgcaa aataaggatt atcaacgggg acaaatttta accctggcag atgcgggtca
      361 ctatgattct gcgctggtta aacttaagca gcttaactct ggagcaccgg acaaagctaa
      421 tttactcgca gaagcctata tctataaact ggcggggcgg catgaggatg aattacgggc
      481 gatgacaggg tcattacctg aaaatgcctt aacgcaacaa tatcccacag aatacgtgca
      541 ggcattacgg aataatcaac ttgctgccgc gattgacgat gccaatttaa cgccagatat
      601 tcgcgctgat attcatgccg aactggtcag actgtcgttt atgcctacgc gcagtgaaag
      661 tgaacgttat gccattgccg atcgcgccct cgcccaatac gctgcattag aaattctgtg
      721 gcatgataac tcagaccgca ctgcccagta ccagcgtatt caggttgatc atcttggcgc
      781 gttattaact cgcgatcgtt ataaagacgt tatttctcac tatcagcgtt taaaaaatac
      841 gaggcaaatt attccgccct gggcgcaata ttgggttgcg tcggcttatc tcaaagatca
      901 gcagccgaaa aaagcacagt caataatgac cgagctcttt tatcacaagg agaccattgc
      961 cccggattta tccgatgaag aacttgcgga tctcttttac agccacctgg agagtgaaaa
     1021 ttatcccggc gcgctaactg tcacccaaca taccattaat acttcgccgc ctttccttcg
     1081 gttaatgggc acgcctacga gcatcccgaa tgatacctgg ttacaggggc attcgtttct
     1141 ctcaaccgta gcaaaatata gtaatgatct tcctcaggct gaaatgatag ccagagagct
     1201 tgcttataac gcaccaggaa atcagggact gcgcattgat tacgcgagtg tgttacaagc
     1261 ccgcggttgg catcgtgcag cagaaaatga attaaaaaaa gcagaagtga tcgagccacg
     1321 taatattaat ctggaggttg aacaagcatg gacagcatta acgttacaag aatggcagca
     1381 ggcagctgtc ttaacgcacg atgttgtcga acgtgaaccg caagatcccg gcgttgtacg
     1441 tttaaaacgt gcggttgatg tacataatct tgcagagctt cgtatagctg gttcaacagg
     1501 aattgatgcc gaaggcccgg atagtggtaa acatgatgtc gacttaacca ccatcgttta
     1561 ttcaccaccg ctgaaggata actggcgcgg ttttgctgga nnnnnnnnnn tctggctcga
     1621 ggcggagtac gctgaacgct ttttcaatca tgagcataaa cccggcgcgc gcctgtctgg
     1681 ctggtatgat tttaatgata actggcgtat tggttcgcaa ctggaacgcc tctctcaccg
     1741 cgttccatta cgggcaatga aaaatggtgt tacaggcaac agtgctcagg cttatgttcg
     1801 ctggtatcaa aatgagcggc gtaagtacgg tgtctcctgg gctttcactg atttttccga
     1861 cagtaaccag cgtcaggaag tctcacttga gggtcaggaa cgcatctggt cttcaccata
     1921 tttgattgtc gatttcctac ccaatctgta ttacgaacaa aatacagaac acgacactcc
     1981 atactacaac cctataaaaa cgttcgatat tgttccggca tttgaggcaa gtcatttgtt
     2041 atggcgaagc tatgaaaata gctgggagca aatattcagc gcaggtgttg gtgcctcctg
     2101 gcaaaaacat tatggcacgg atgtcgtcac ccaactcggc tacgggcaac gtattagctg
     2161 gaatgacgtg attgatgctg gcgcaacgct acgctgggaa aaacgacctt atgacggtga
     2221 cagagaacac aacttatacg ttgaattcga tatgacattc agattttaag gataaatatg
     2281 ttacgtaatg gaaataaata tatcctgatg ctgg
//
LOCUS       DAWDRC010000617         2310 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16744_length_2310_cov_2.251441, whole genome shotgun sequence.
ACCESSION   DAWDRC010000617 DAWDRC010000000
VERSION     DAWDRC010000617.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16744_length_2310_cov_2.251441"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          583 a          552 c          620 g          555 t
ORIGIN      
        1 agagacgcaa agataaagat gacggcaaag catatgtgct aagccgtcag tgacatcagg
       61 cgtaatgctc tgccatttcg ttgaggattt gctcgcacca gctttgaata cgctcgtcgc
      121 taaggtcata ctggttagtt tcatccagcg ccagacccac gaacagttgc ccgtcagcaa
      181 tcaccggttt cgggctggta aattcatatc cttccgttgg ccagtagccg acgaacttca
      241 cgcctttggt cgagagtttg tcatgcagca taccgagcgc atcgaggaac cactcgccgt
      301 atcccagttg atcgccaagc ccatacagcg caacaatttt accttcgagg ttcaggtcgt
      361 cgagctgatc ccagacggct tcccagtctt cctggatttc gccaaaatcc caggtcggga
      421 tacccaggat gagcacatcg tactgctcca ttaatttcgg ggagtcgtcc ttgaggttat
      481 gtaaggtcac cagttctggg ccgataatat cgcggatttt ttctgccgcc atttcggtgt
      541 aacaggtgct ggaaccgtaa aaaagaccca tattcataac gtaagcttct cgattctgct
      601 gtggcttttg cggctagtgt atcagaatcg ctttagtcac ggcacaataa ggcataatgg
      661 atcaaatgag tgaccataag gggcgcaagt gaaacaggat ctggcacgca tcgagcagtt
      721 tcttgatgct ctgtggctgg aaaaaaatct ggctgaaaat acgttgaacg cttaccgtcg
      781 cgatctgtca atgatggtgg agtggttgca tcaccgcggg ttgacgctgg cgacggcgca
      841 aagtgacgat ttgcaggcat tactggcaga acggctggag ggcgggtata aagccaccag
      901 ctcagcgcgt ttgctgagtg cagtgcgccg attgttccag tatctttatc gcgaaaagtt
      961 tcgtgaagat gatcccagtg cgcatctcgc ttcaccgaaa ttgccccagc gtttgccaaa
     1021 agatttaagc gaagcgcagg tcgaacgttt attacaggca ccattaattg atcagccact
     1081 ggagctacgc gataaagcca tgcttgaagt gttgtatgct accggactgc gtgtctctga
     1141 actggtcggg ctgacaatga gtgatatcag cctgcgtcag ggcgtggtgc gggtcattgg
     1201 taaaggcaac aaagagcgcc tggtgccgtt aggtgaagag gcggtttact ggctggaaac
     1261 ctatctggaa catgggcgtc cgtggctgtt gaatggtgtg tcaattgacg tgttgtttcc
     1321 cagccagcgt gcgcagcaga tgacgcgaca gaccttctgg caccgcatta aacattatgc
     1381 cgtgctggcg ggtatcgaca gcgaaaagct gtcaccgcat gtgttgcgtc acgcttttgc
     1441 cactcactta ttaaatcatg gtgcggattt gcgcgtggtg cagatgctac tgggccacag
     1501 cgatctctcc accacgcaaa tttatacgca tgtcgctacc gagcgtctgc ggcaacttca
     1561 tcaacagcat cacccgcggg cgtgatgtct gaaaagaaca ggaaaattta tgaagaaagg
     1621 ttttatgtta tttaccttgt tggcagcgtt ttcaggcttt gttcaggctg atgacgcggc
     1681 aattcaacaa acgttagcca aaatgggcat caaaagcagc gatattcagc ccgcgcccgt
     1741 agctggcatg aagacagttc tgactaacag cggtgtgttg tacatcaccg atgatggtaa
     1801 acatatcatt caggggccaa tgtatgacgt tagtggcacg gctccggtca atgtcaccaa
     1861 taagatgctg ttaaagcagt tgaatgcgct tgaaaaagag atgatcgttt ataaagcgcc
     1921 gcaggaaaaa cacgtcatca ccgtgtttac tgatattacc tgtggttact gccacaaact
     1981 gcatgagcaa atggcagact ataacgcgct ggggatcacc gtgcgttatc ttgctttccc
     2041 gcgccagggg ctggacagcg atgcagagaa agaaatgaaa gctatctggt gtgcgaaaga
     2101 taaaaacaaa gcgtttgatg atgtgatggc aggtaaaagc gtcgcaccag ccagctgcga
     2161 cgtggatatt gccgaccatt acgcacttgg cgtccagctt ggcgttagcg gtactccggc
     2221 agttgtgctg agcaatggca cacttgttcc gggttaccag ccgccgaaag acatgaaaga
     2281 attcctcgac gaacaccaaa aaatgaccag
//
LOCUS       DAWDRC010000618         2303 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16806_length_2303_cov_1.484875, whole genome shotgun sequence.
ACCESSION   DAWDRC010000618 DAWDRC010000000
VERSION     DAWDRC010000618.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16806_length_2303_cov_1.484875"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    239..248
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1258..1267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1845..1871
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          530 a          565 c          633 g          528 t
ORIGIN      
        1 tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc
       61 attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggt
      121 gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt atgtgcataa accgacactt
      181 gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggnn
      241 nnnnnnnngc atggtttacg cgccgaccca ggagcacggc aagcaggtta aaatcgaaga
      301 cgtaccgctg gaacagcgtg acatcttctc gctgaccaac gaagaagtgc aggaactggc
      361 aaaacaggcc gtacaaattg agaaacacta cggtcgcccg atggatattg agtgggcgaa
      421 agatggccac accggcaaac tgttcattgt gcaggcgcgt ccggaaaccg tgcgctcacg
      481 cggtcaggtc atggagcgtt atacgctgca ttcacagggt aagattatcg ccgaaggccg
      541 tgctatcggt catcgcatcg gcgcgggtcc ggtgaaagtc atccatgaca tcagcgaaat
      601 gaaccgcatc gaacctggcg acgtgctggt tactgacatg accgacccgg actgggaacc
      661 gatcatgaag aaagcatctg ccatcgtcac caatcgtggc ggtcgtacct gtcacgcggc
      721 gatcatcgct cgtgaactcg gcattccggc ggtagtgggc tgtggtgatg caacagaacg
      781 gatgaaagac ggcgagaacg tcactgtttc ttgtgccgaa ggtgataccg gttacgtcta
      841 tgcggagttg ctggaattta gcgtgaaaag ctccagcgta gaaacgatgc cggacctgcc
      901 gttgaaggtg atgatgaacg ccggtaaccc ggaccgtgct ttcgacttcg cctgcctgcc
      961 gaacgaaggc gtgggccttg cgcgtctgga atttatcatc aaccgtatga ttggcgtcca
     1021 cccacgcgca ctgcttgagt ttgacgatca ggaaccgcag ttgcaaaacg aaatccgcga
     1081 gatgatgaaa ggttttgatt ctccgcgtga attttacgtt ggtcgtctga ctgaagggat
     1141 cgcgacgctg ggtgccgcgt tttatccgaa gcgcgtcatt gtccgtcttt ctgattttaa
     1201 atcgaacgaa tatgccaatc tggtcggtgg tgagcgttac gagccagatg aagagaannn
     1261 nnnnnnnacc cgatgctcgg cttccgtggc gcgggccgct atgtttccga cagcttccgc
     1321 gactgcttcg cgctggagtg tgaagcagtg aaacgtgtgc gcaacgacat ggggctgact
     1381 aacgttgaga tcatgatccc gttcgtgcgt accgttgatc aggcgaaagc ggtggttgag
     1441 gaactggcgc gtcaggggct gaaacgtggc gagaacgggc tgaaaatcat catgatgtgt
     1501 gaaattccgt ccaacgcctt gctggcagag cagttcctgg aatatttcga cggcttctca
     1561 attggctcaa acgacatgac gcagctggcg ctcggtctgg atcgtgactc cggcgtggtg
     1621 tccgaactgt tcgatgagcg caacgatgcg gtgaaagcac tgctgtcgat ggcgattcgt
     1681 gccgcgaaga aacagggcaa atatgtcggg atttgcggtc agggtccatc cgaccacgaa
     1741 gactttgctg catggttgat ggaagagggg atcgatagcc tgtctctgaa cccggacacc
     1801 gtggtgcaaa cctggttaag cctggccgaa ctgaagaaat aaaannnnnn nnnnnnnnnn
     1861 nnnnnnnnnn ncccccaagt atgaaacttt ttcaatctct tcacagactt cctgagttaa
     1921 tcgccgcata atatctttcc ttaacaaaaa cttttgtatt ttacctgagg cagtgcgcgg
     1981 tagtttttcg attaccacga tatgttcagg atatttatat ttcgcgaccc gtttacggct
     2041 aaaaaatgcc actacatctt ccagcgataa tgaatgatgc ggtgctttca gcacgacata
     2101 agcgcatgaa cgttcaccta agcgttcatc gggcattgca accacacaag catcgtgaat
     2161 tttaggatgc tgcaataaaa tatcttccac ttcacggctg ctaatatttt cgccgccgcg
     2221 gacaataata tctttcttgc gcccggttat ttttatatag ccagcctcat ccatgcggca
     2281 gagatcgccg ctgtaatacc agc
//
LOCUS       DAWDRC010000619         2288 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16909_length_2288_cov_2.763995, whole genome shotgun sequence.
ACCESSION   DAWDRC010000619 DAWDRC010000000
VERSION     DAWDRC010000619.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16909_length_2288_cov_2.763995"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          584 a          578 c          510 g          616 t
ORIGIN      
        1 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaattagaaa
       61 aaccccggtc aataaggccg gggttttttg catatcaatt atttgcatga tgaagggaat
      121 ctcatgtcag ttctgtatat ccaaattcgt cgtaatcaaa ttactgttcg cgatcttgaa
      181 agcaaacgtg aagtgtcagg agatgctgcc ttcagtaacc agcgcctgtt aatcgccaat
      241 ttctttgttg cagaaaaagt tctgcaagat cttgttctgc aactccaccc acgttcaacc
      301 tggcattctt ttttgccagc aaaacgtatg gatattgttg tgagcgcgct ggaaatgaat
      361 gagggcggtt tgtcacaggt tgaggaacgc attcttcatg aagtggtcgc aggggcaacg
      421 ttaatgaaat atcgccagtt ccacatccat gcgcaatcag tggtgctcag tgatagtgcg
      481 gtcatggcaa tgtttaagca gaaataatca tgtcaccatt ggtgggtacg aaacctaaag
      541 ttcagcccac cgggatgaga aaaaaccgcc tacgccccca catatgccag attcagcaac
      601 ggatacggtt tccccaaatc gtccacctca gagcgttccg taaccttaaa gcccaccttc
      661 ttatagaacc caaccgcctg ctcattttgc tcattaacat tggttgtcag ttccggggcc
      721 attgagagcg catgcttcac cagcatccgg cccacgccgc agccgcgcac atcaggatcg
      781 ataaacagcg catccatatg ctgcccactt aacaacataa acccaaccgg ctgatcccgc
      841 tcattaaccg cgacccacaa cggtgcttcc ggcagaaagg aatgcaccag ctcttccagc
      901 tcggcccgat actctgctga taaaaaatcg tgagtggcat cgacagaacg acaccaaatc
      961 gcaacgagtt ccccccccct cctcatgccg tgagcgtcga atactaataa ccattttctc
     1021 tccttttcgt cattcttata ttctaacgta gccttttcct tgaaactttc tcaccttcaa
     1081 catgcaggct cgacattggt aaattttctg gttatcttca gctatctgga tgtctaaacg
     1141 tataagcgta tgtagtgagg taatcaggtt atgccgattc gtgtgccgga cgagctaccc
     1201 gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga caacttctcg cgcgtctggt
     1261 caggaaattc gtccgctaaa ggtgcttatc cttaacctga tgccgaagaa gatcgaaact
     1321 gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc aggtcgatat tcagctgttg
     1381 cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc atctgaataa cttctactgt
     1441 aactttgaag atattcagga acaaaacttt gacggtctga tagttaccgg cgcgccgctg
     1501 ggcctggtgg agtttaatga tgtcgcttac tggccgcaga tcaaacaggt gctggagtgg
     1561 tcgaaagatc acgtcacctc gacgctgttt gtctgctggg ctgtacaggc cgcgctcaat
     1621 atcctctatg gtattcctaa gcaaacccgc accgacaaac tctctggcgt ttacgagcat
     1681 catattctcc atcctcatgc gcttctgacg cgtggctttg atgattcatt cctggcaccg
     1741 cattcgcgct atgctgactt tccggcagcg ttgattcgtg attataccga tctggaaatt
     1801 ctggcagaga cggaagaagg ggatgcatat ctgtttgcca gcaaagataa gcgaattgcc
     1861 tttgtgacgg gccatcccga atatgatgcg caaacgctgg cgcaggagta tttccgtgat
     1921 gtggaagccg ggctagaccc ggatgtaccg tataactatt tcccgcacaa tgatccgcaa
     1981 aatacaccgc gagcgagctg gcgtagtcac ggtaatttgc tgtttaccaa ctggctcaac
     2041 tattacgtct accagatcac gccatatgat ctacggcaca tgaatccaac gctggattaa
     2101 tcttctgtga tagtcgatcg ttaagcgatt cagcacctta cctcaggcac cttcgggtgc
     2161 cttttttatt tccgaaacac acctcagcaa gtgaataaat tttatttata ttgttatcag
     2221 taagttaaca tgtattttta attaaaatgg aaattgtttt tgattttgca ttttaaatga
     2281 gtagtctt
//
LOCUS       DAWDRC010000620         2279 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16974_length_2279_cov_1.793615, whole genome shotgun sequence.
ACCESSION   DAWDRC010000620 DAWDRC010000000
VERSION     DAWDRC010000620.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16974_length_2279_cov_1.793615"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    207..216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          480 a          678 c          601 g          510 t
ORIGIN      
        1 ttgtgtttgg gcagcaggaa cgcctgacga atccccgcca ccagcgcctc gaccgattcc
       61 ggttccacgc aaaccgcaat gcccggaaag gtttcgcaaa gctgccccag ttctgtgtgg
      121 gcttctgcgg taatcaccgc gttaccgcct accgccagaa tattggtcag tttcgacggc
      181 aatacggcat ctgccgcgcc gcgtttnnnn nnnnnngcgg gtaaagcgtc atacgattgc
      241 agcggaaaaa attgcatgtt gcgcagtcca cgctgctgcg ccattttttc cagccgcgct
      301 ttgccgccgc cctgcccgac aatggcaaaa ataagcggtt catcgcgcag gcgatcggca
      361 gcttcaataa cgttttccag cccctgcttt tcaccaatat tgccggagta aagaatgatt
      421 tttttgttat ccggcaggcc aagctggtta cgaagggcat caacatcggc gtctgcgaca
      481 tgctgaaaac gggcaatttc tgaccagttt gggaagaaaa tgacgttttc cgccgccacg
      541 cctttctcga tagctttatt catcatcgaa cgcgaaatcg tggagacgtt atcgacgtta
      601 tgcagtccgc tacgttcgaa cgccgttgcc agctgtgcca ctttgccgcc tttacctttt
      661 ccggcaaggc ccagccccag catggcgtcc acttcgtaat cctgaatatg gagcaccgtg
      721 cgcgcaccag acagtttcgc cagcaggcgc attcctggcg tgcaaaacag cgttggcacc
      781 acgccgataa tgcgatccgg cttccagcca tcagcggaaa gaaactgctg gcggcaaaac
      841 tgccgagatg caacaggcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc
      901 gccacaccgt ggcggccccc tcttcccgtt tgtaccgcca ggcggaatag ttctcgccca
      961 cctgccactg cgggtagtaa ggcggtgcgg taataacccg cacctcatga ccttgtgccg
     1021 ccagccattc caccatctcg ccggtgtatt tgccgatgcc tgttaactcc ggcgagtagt
     1081 taatgccgta gaccagtatt ttcataatcc gggtactccg gcacgcttct cagagagaaa
     1141 ataggcgcgg ctgttagcgt gaacattgtc actggcgagc aacgcgtccg gcgtcagcca
     1201 gcggtaatcg tcatgctgct catccggcag taacagctca tcttccgcga ctctgatgcg
     1261 aaaaccgagc atagtgagtg gtgaattccg tgccggagaa gttatcgtca taaaagtgct
     1321 gccagacacc gtaaaactgg cctgctgtta tcggcagacg cagccccagt tccgccatcg
     1381 tcagccgctc aaatgcggct tccagcgttt cgtctttctg cacgcgccct cccggcacaa
     1441 accagtaacc ctgcgccggg cggttggttc ttttgccaag cagaaactcg ccgcgactgt
     1501 tctcaacaat aaagtcgaga gagacaagcg gtgtggagcg cactaccgtg gcaaagtctt
     1561 cctgacgtaa aaacatcatt acccccgaaa gcggtcttga ttctcaagga accactggta
     1621 agtgctggca agccccgctt ccagtgagat ttcgtgatac cagccaagct gatgcaaccg
     1681 cgtcacatcc agcagtttgc gcggcgtacc atccggttta ctggcatcaa aaaccacccg
     1741 acctttgtaa cccaccactt tggcgatggt ttgcgccagc tcacggatag tgcagtcaac
     1801 gcccgtgccg acgttaatgt gcgacagcat cggccgggtg ttctcctgcc agacctcgtg
     1861 cgccagctcc atgacatgaa tgctcgccgc tgccatatca tcaacgtgta gaaattcgcg
     1921 catcggcgta ccgctgcccc acaccaccac gtccggcgca ttctgtgccg tcgcctcatg
     1981 gaagcgacgc agcaatgctg ggatcacatg cgaattactc gggtggaagt tgtcgtgtgg
     2041 cccgtacagg ttggttggca tgactgagcg gtaatcccgt ccgtactggc ggttgtatga
     2101 ttcacacagt ttgatcccgg cgattttggc aatggcataa ggctcgttgg tcggctccag
     2161 cgtgccctgc aacaactcgc tttctgccat cggctgtttt gccagtttcg ggtagatgca
     2221 ggacgatccg agaaacagca gtttgttcac gtcgttctga tgcgcggcgt gaatgatgt
//
LOCUS       DAWDRC010000621         2277 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_16993_length_2277_cov_1.986499, whole genome shotgun sequence.
ACCESSION   DAWDRC010000621 DAWDRC010000000
VERSION     DAWDRC010000621.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16993_length_2277_cov_1.986499"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          585 a          599 c          636 g          457 t
ORIGIN      
        1 cgccgatggc ggataaatct cgctataccg gacacttgat cgactttaac gtccgtgcag
       61 aacgcatggg ctggctgccg tctgcgccgc agttaggcac taacccgctg actatcgctc
      121 gcgaagcaga aaaagccggg atgaatccgg tggactatac ggtgaaatcc ctgaaagagg
      181 gttctatccg tttcgctgcg gaacagccgg aaaacggtaa aaaccacccg cgtaacctgt
      241 tcatctggcg ttctaacctg ctcggttctt ccggtaaagg tcatgagttt atgctcaagt
      301 acctgctggg gacggagcac ggtatccagg gcaaagacct gggtcagcag ggcggcgtga
      361 agccggaaga agtggactgg caggacaacg gtctggaagg caagctggat ctggtggtga
      421 cgctggactt ccgtctgtcg agcacctgtc tctattccga catcattttg ccgacggcga
      481 cctggtacga aaaagacgac atgaatactt cggatatgca tccgtttatt cacccgctgt
      541 ctgcggcggt cgatccggcc tgggaagcga aaagcgactg ggaaatctac aaagccatcg
      601 cgaagaaatt ctccgaagtg tgcgttggtc atctgggtaa agaaactgac atcgtcacgc
      661 tgcctatcca gcacgactct gccgctgaac tggcgcaacc gctggatgtg aaagactgga
      721 aaaaaggcga atgcgacctg atcccaggta aaactgcacc gcacattatg gtcgtagagc
      781 gcgattatcc agcaacttac gaacgcttta cctctatcgg cccgctgatg gagaaaatcg
      841 gtaacggcgg taaagggatt gcctggaaca cccagagcga gatggatctc ctgcgtaagc
      901 tcaactacac caaagcggaa ggtccggcaa aaggccagcc gatgctcaat accgcaattg
      961 atgcggcaga gatgatcctg acgctggcac cggaaaccaa tggtcaggta gctgtaaaag
     1021 cctgggctgc cctgagcgag tttaccggcc gtgaccatac gcatctggcg ctgaataaag
     1081 aagacgagaa gattcgcttc cgcgatattc aggcacagcc gcgcaaaatc atctccagcc
     1141 cgacatggtc tggccttgaa gatgaacacg tttcttacaa cgccggttac accaacgttc
     1201 acgagctgat cccgtggcgt acgctctctg gccgtcagca gctgtatcag gatcaccaat
     1261 ggatgcgtga tttcggtgaa agcctgctgg tttatcgtcc gccgatcgac acccgttcgg
     1321 tgaaagaagt gatgggccag aaatccaatg gcaacccgga aaaagcgctc aacttcctga
     1381 cgccacacca gaagtggggt atccactcca cctacagcga caacctgctg atgctgactt
     1441 taggtcgcgg tggtccggtg gtctggctga gtgaagccga tgccaaagag ctgggtatcg
     1501 ccgataacga ctggattgaa gtcttcaaca gcaacggtgc actgactgcc cgtgcggttg
     1561 tcagccagcg tgttccggca gggatgacca tgatgtacca cgcgcaggaa cgtatcgtta
     1621 acctgcctgg ttcggaaatt acccaacagc gtggtggtat ccataactcg gtcacccgta
     1681 tcacgccgaa accgacgcat atgatcggcg gctatgccca tctggcatat ggctttaact
     1741 actacggcac cgtaggttct aaccgcgatg agtttgttgt agtgcgtaag atgaagaaca
     1801 ttgactggtt agatggcgaa ggcaatgacc aggtacagga gagcgtaaaa tgaaaattcg
     1861 ttcacaagtc ggcatggtgc tgaatctcga taagtgcatc ggctgccaca cctgttcagt
     1921 gacctgtaaa aacgtctgga ccagccgtga aggcgtggaa tacgcgtggt tcaacaacgt
     1981 ggaaaccaag ccgggccagg gcttcccgac tgactgggaa aaccaggaaa aatacaaagg
     2041 cggctggatc cgtaaaatca acggcaaact gcagccgcgc atgggtaacc gtgccatgct
     2101 gctgggtaaa atcttcgcta acccgcatct gccggggatc gacgattatt acgagccgtt
     2161 cgattttgat tatcagaacc tgcataccgc gccggaaggc agcaaatcac agccgattgc
     2221 ccgtccgcgt tcactgatta ccggcgaacg gatggcgaaa atcgaaaaag ggccgaa
//
LOCUS       DAWDRC010000622         2276 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17001_length_2276_cov_2.167042, whole genome shotgun sequence.
ACCESSION   DAWDRC010000622 DAWDRC010000000
VERSION     DAWDRC010000622.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17001_length_2276_cov_2.167042"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1194..1203
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1658..1667
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          482 a          640 c          619 g          515 t
ORIGIN      
        1 aatgccgtct gctgatgccg gtacttccag taccactttg tcagtttcga tttctaccag
       61 cacttcatca cgtacgactg cgtcgccggg ttttttatgc caggttgcga cggtggcatc
      121 ggctacggat tcaggcaggt cagggaccag aatatctacg ctactcattg tgtatccttt
      181 atttattcga cgttcagcgc gtcattaacc agatcttgtt gctgtttctg gtgaacggac
      241 atatacccta ccgccggaga ggcggaggct gggcggcctg cataacgcag agaagcccca
      301 aacggaatca cttcacggaa atgatgctgg ctgcagtacc atgcgccctg gttgagcggc
      361 tcttcctggc accagacaaa atccttgacg tgagcaaact gctgcaacac ttcctgcatc
      421 gctttatgcg ggaacgggta gagttgctcg atacgcacaa tggcgacatc gtgttgattg
      481 ttcttacgac gttgttccag caggtcgtaa taaaccttac cagaacacat cactacgcgc
      541 ttcacgccct tcggatcaag ctcgtcgatt tcaccgatgg ctggcaggaa ggtgccgttc
      601 gccagttctt cgaggctgga taccgccagc ggatgacgca gcagggattt cggcgacatc
      661 acgaccagtg gacgacgcat cccgcgcaac gcctgacgac gcagcatgtg gtaaacctgt
      721 gccggggtag aaggtacgca aacctgcatg ttttgctcag cgcaaagttg cagataacgt
      781 tccagacgcg cggaggagtg ctccggcccc tgcccttcgt aaccgtgcgg cagcaacatc
      841 accaggccac acatccggcc ccatttctgt tcgccagagg agatgaactg gtcgataacc
      901 acctgtgcac cgttggcgaa gtcaccgaac tgtgcttccc agatggtcag ggtacgtggt
      961 tctgcggtgg cataaccgta ttcaaacgcc agtacggctt cttcagacag tacagagtcc
     1021 cagacgcgga acgcgccctg gccgttatgg atatgttgca gcggcgtgta agtggaaccg
     1081 ttagactggt tgtggatcac cgcgtggcgg tggaagaagg taccgcgacc ggagtcttca
     1141 cccgacaggc gaaccggaat gccttcgtca accagcgtgg cgtaagcgag gttnnnnnnn
     1201 nnnttccgcg ccgccccagt cgaacagttt ctcaccggca gccatcgcct ggcgatcgcc
     1261 ataaatcttg gcaacgcgag actgcatttc aaccgcttcc ggcaccgtgc tgatgcgttt
     1321 agccagttcc tgcaggcgct tcatctcaac tttgttcggg tactcttcgt cccattcatg
     1381 gttgaggtac ggcgaccagg tgaaagagtg catgttcatc ggacgccact ctgccacaac
     1441 gcaatcgcca gcatccagcg catcgcggta caggttaacc atctcggtgg catcttccag
     1501 cgtcgcgact ttttcctgct ccagtttgtc agcgtagatt ttgcgcggcg tcggatgttt
     1561 tttgattttc tgatacatca gcggctgggt tgcgctcggc tcgtcggctt cgttgtggcc
     1621 gtgacggcgg tagcatacca ggtcgatgaa gacatcannn nnnnnnntta cggaaatcga
     1681 gcgccagacg ggtaacaaag gcaacggctt ccggatcatc cgcgttaacg tggaaaatcg
     1741 gtgcctgaac catcttaccg atatcagtac agtacggcgt cgaacgggca tccagcgggt
     1801 tagaggtggt gaagccaacc tggttgttga taacgatacg taccgtaccg ccaacttcat
     1861 aaccacgcgc tttcgacatg ttcagggttt cctgaactac gccctgcccg gtcactgcgg
     1921 cgtcaccatg aatggtgatt ggcagcactt tgttgctgct cggctcatca agtctgtcca
     1981 gacgggcacg aacagaaccg ataacgaccg ggcttacaat ctcaaggtga gacgggttaa
     2041 acgccagcgc caggtgaacc aggccgccat cggtctggaa gtcagacgag aagcccatgt
     2101 ggtatttcac gtcgcccgtg ccgaggtgtt ctttatgttt accggcaaac tcgtcgaaca
     2161 agtcttgcgg ttttttaccc agcacgttca ccagaacgtt cagacgacca cggtgcgcca
     2221 tcccgagaac cacttcacgg gtgccgctgt tgccagcgtg gcggatcatc tcttta
//
LOCUS       DAWDRC010000623         2266 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17077_length_2266_cov_2.463139, whole genome shotgun sequence.
ACCESSION   DAWDRC010000623 DAWDRC010000000
VERSION     DAWDRC010000623.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17077_length_2266_cov_2.463139"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          562 a          606 c          587 g          511 t
ORIGIN      
        1 tgcgccacct gacgtacgcc aatttcatgc gccgcgccgc cgaggttaaa gaagcagtaa
       61 tgcacgcgac agccgcgacg catcaacata taactggaaa caccggagtc gaaaccaccg
      121 gaaatgagcg acaacacatc ttcctgggtg ccgatcggga aaccgccaat accttcgtag
      181 cggcctttaa tcagcagcag acgatcgtct tccacttcca gatggacagt cacttccgga
      241 ttggtcagct tcacgcgcgc ggattcaata tgctgattta aaccgccgcc gacgtaacgt
      301 tccacatcaa tcgagctaaa atcatgtttg ccgcggcgct tcacgcgtac gcaaaaggtt
      361 ttgccttcca gctgatcgcg atattgaacc aacgctttct cgaaaatatc gtgcatgtca
      421 gtaaacggca cgtcttcgac ttcgagaata tggtggatac ccggaatacg ggtcagggcg
      481 tcgcgaatag ccagacgctg gttttcatct tttgcgcgaa cttcgatgtt atcccagtgg
      541 cggacgacag cgagcgtctc atcatagtgc tttaaaacgt tacgaatgtt cccggtaagg
      601 atttttataa agcgcaagcg cacagattgg cttttgatgg tgatttccgg gaacaattta
      661 atgataaact tcatggcggc aatggttcgt tggcaagcct taagcgactt gtatagggaa
      721 aaatacagca gcccacacct gcggctgcat ccaggcgcgg aagtatacca ctaacatcgc
      781 tttgctgcgc acatcacctt accattgcgc gttatttgct atttgccctg agtccgttac
      841 catgacgggg cgaaaaatat tgagagtcag acattcatta tgccgaagaa aaatgaggct
      901 cccgccagct ttgaaaaggc gctgagcgag ctggaacaga ttgtaacccg tctggaaagt
      961 ggcgacctgc cgctggaaga ggcgctgaac gaattcgaac gcggcgtgca gctggcacgt
     1021 caggggcagg ctaaattaca acaagccgaa cagcgcgtac aaattctgct gtctgacaat
     1081 gaagacgcct ctctaacccc ttttacaccg gacaatgagt aatggacttt ccgcagcaac
     1141 tcgaagcctg cgttaagcag gccaaccagg cgctgagccg ttttatcgcc ccactgccct
     1201 ttcagaacac tcccgtggtc gaaaccatgc agtatggcgc attattaggt ggtaagcgcc
     1261 tgcgaccttt cttggtttat gccactggtc atatgttcgg cgttagcaca aacacactgg
     1321 acgcacccgc tgccgccgta gagtgtatcc acgcttactc attaattcat gatgatttac
     1381 cggcgatgga tgatgacgat ctgcgtcgcg gtttgccgac ctgccatgtg aagtttggcg
     1441 aagcaaacgc gattctcgct ggtgacgctt tacaaacgct ggcgttctcg attctaagcg
     1501 atgccgatat gccggaagtg tcagatcgcg acagaatttc gatgatttct gagctggcga
     1561 gcgccagcgg tattgccgga atgtgcggtg gtcaggcatt agatttagac gctgaaggca
     1621 aacacgtacc tctggacgcg cttgagcgta ttcatcgtca taaaaccggc gcattgattc
     1681 gcgccgccgt tcgccttggt gcattaagcg ccggagataa aggacgtcgt gctctgccag
     1741 tactcgacaa gtacgcagag agcatcggcc ttgccttcca ggttcaggat gacatcctgg
     1801 atgtggtggg agatactgca acgttgggaa aacgccaggg tgccgaccag caacttggta
     1861 aaagtaccta ccctgcactt ctgggtcttg agcaagcccg gaagaaagcc cgggatctga
     1921 tcgacgatgc ccgtcagtcg ctgaaacaac tgactgaaca gtcactcgat acctcggcac
     1981 tggaagcgct agcggactac atcatccagc gtaataaata aacaataagt attaataggc
     2041 ccctgatgag ttttgatatt gccaaatacc cgaccctggc actggtcgac tccacccagg
     2101 agttacgact gttgccgaaa gagagtttac cgaaactctg cgacgaactg cgccgctatt
     2161 tactcgacag cgtgagccgt tccagcgggc acttcgcctc cgggctgggc acggtcgaac
     2221 tgaccgtggc gctgcactat gtctacaata ccccgtttga ccagtt
//
LOCUS       DAWDRC010000624         2263 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17108_length_2263_cov_2.014493, whole genome shotgun sequence.
ACCESSION   DAWDRC010000624 DAWDRC010000000
VERSION     DAWDRC010000624.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17108_length_2263_cov_2.014493"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          519 a          548 c          584 g          612 t
ORIGIN      
        1 tttttaaagc aaatgttttt gggggcttca gcgcccccaa aagcgagtga cattaatttg
       61 gggttacgat atccacccac aaatcgaaat tatccagaca ggcgataatt tcttccagtt
      121 tcgcgccatt gccaataatt ttggctttct tcgctttcac ctgatcggcc atttttgcct
      181 gtccggtcag cacggcgtgc agatcttcac ggctaatgta gaaagaggcg tcggcttgcg
      241 attgcagcgt tttacggtag ttaagcacgc tatcgttcag catcaggttg aggttatcgc
      301 cgttgctcat attgaagttc aggctgatat ttttacctgc ggctttcgtg ctatcgaggc
      361 gaacggccat aaagtcgaac agcatttcga ccgacatccc acgaatggtg tccggggaac
      421 cggtggtgcc gtggctgaac ttatgcaccc cttcgcgcag ctctttcgcg ccggtcaggt
      481 agaaaccgcg ccatgtggcg gactcggcct gatagcccag ctgttcaaag ttattcgctt
      541 gcagattctt cgcgacctgg tcacccgggt tggcggcaat cacctgtttc agcagttctg
      601 ccgaccagcg ataatcacct tgcttgttcg cttcttgcgc caggttgatg acgcgggcag
      661 aaccgcccag cgcctgcacg taacgtttac ccatctccac ctgaccatac ggatgcaggt
      721 tagccgggtt accgtcgtaa tagccaagat agaagttata caccgcgcgg gcgttgtggc
      781 tgacagaacc gtaataaccg cggctggccc agttattggc aagcgcaggc ggcagcttaa
      841 tcatgtcgcc gatttcattc atggtgtagc cctggttcgc caggtgcagg gtctggtcgt
      901 gaatgtactt gatagtatcg cgatatttac caatataatc attgatatgc ttattgcccc
      961 agaccggcca ggtgtgcggc ataaacagta cttccgcgtc gttaccccac atatccaacg
     1021 tttcgttcag atactcggtc cacttgctgg tgtcgcgggt tttcgcgccg cgcagagtgt
     1081 agaagttgtg cagggtatgc gtggcgttct cggcggtaca cagggctttc agggccggaa
     1141 tatagaagtg catttcggct ggcgcttcgc tacctggggt catcaggaag tcaaactcca
     1201 ggccgtcgat aatcatcttc tcgccagttc tgacgatagt tttagtcggt gcaataatgc
     1261 tcgggtcgcc cgttgccagc gtcacgccaa ggccattacc cacattgcct tgcgcgttgt
     1321 gcggcagtaa cagaccgtaa gagtagagcg cacggcggct catgatgtta cccgccagca
     1381 cgttttcgct gatggcttcg tccataaagc cagctggagc aatcacctga actttgccgg
     1441 atttaacatc ggcttcagag atgatgcctt tcacgccgcc atagtggtcg gcgtggctgt
     1501 gggtatagat aacggcaata atgggttttt gcgggcgatg ctggaagtaa aggtcaagtg
     1561 cggcttttgc ggcaggtggc gtcaccagtg ggtcaataac aataatgcct ttctcgccct
     1621 caacgaacgt aatgttagag atatcctggc cgcgcacctg atacatttta tcggtgactt
     1681 tgaacaggcc tgaaatgccg ttgatctgcg actgacgcca caggctgggg tttacggttt
     1741 ccggcgcagc ggcattaata tcaaatttgt aatcattcgc gcggtagtaa attttaccgt
     1801 tcgcatcacg cagaatacct tcatccagca gcggagcgat aaatccacgc tgagcatcgt
     1861 caaaatcctg acgatcgcta aacggtaatg atttggcgta attctgatta acctgttggg
     1921 tatattgagt ggcggctttc acttcatctg ttgcgtaagc ggtatttatc atcatgctgc
     1981 tggcggcagt gaacgcaata acaatcctgc tcaaacggaa taaccgagag ttattcatat
     2041 aaatctccat ttatcattta tgtcgtaaat atgtaatcca tgcaaaatac gtcatttaaa
     2101 gctggcgtta tcataattct gaaattgcga ctttaataag tggaagtgtg agcggaacga
     2161 gccattttat taggcatttg tgctgaagag ggaaaattac agtagaacta tcattgctgg
     2221 gaataatatg ggctgttgta tgtaattttt atatatattg tac
//
LOCUS       DAWDRC010000625         2262 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17113_length_2262_cov_2.146806, whole genome shotgun sequence.
ACCESSION   DAWDRC010000625 DAWDRC010000000
VERSION     DAWDRC010000625.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17113_length_2262_cov_2.146806"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          679 a          481 c          503 g          599 t
ORIGIN      
        1 caactgctgc tgttgctgct gaattgcact gcggtcaccc ggagacagca tggctaccgg
       61 agacgaaaat gcggggataa aaaaaccaga aaaaacggag agggagagat agcatatcca
      121 cggagagccg cgccggaaca tatcacaaca ggggacagac aacgcacggg ataaaaggga
      181 cgttttcgtc agaatgttgt tctgccggcg ctgcatgttc tccccgtatt ctggcctgtg
      241 tgatgccagt gtaaattcat gtcaaaaagt gtcagcgaga aaatttacac ctgaaaaagt
      301 ctgtcatgaa acagttttat gacaagacat caaaaaaatg tcaaaaacgt gatctttctt
      361 cagcttttca gaatattgag taccaggaag tcaatatcaa ttctgggaca catatgcatt
      421 taccattaaa accgggcaca acaagctgtt cagtctcaat ccataccccg tgttttatca
      481 atatcagaat aagaatatct gcggataaat aaaaaaggaa tgtcgaggga gcttaccgca
      541 gagggctctc cacctcacaa gccatttaca aaaatgatac cggcatatct ttggtccctg
      601 tcacaaccag acattgacaa tccaatataa ccccaaatat atttcgggta agaagtctgg
      661 gatttataca gatatactgt ctgttattac tatttaatta tataattgta ttgcatacat
      721 aagaggcact aatgaaacgt tatataaaat ggtttgccat cacaattttt atcagtatgt
      781 tgagtgcctg tgtccgtacg gccccagtgc aacagataag caccactgtc agtgtgggtc
      841 atactcagga gcaggttaaa aatgccattt tgaaagcagg tgcgcagcgc aagtggatta
      901 tgacgcaagt gtcccctgga gttattaaag ctcgctatca aacacgaaat cacgttgcag
      961 aggttcgtat tacatataca gctacctact ataacatcaa atatgacagt agcctgaatc
     1021 tgcaggcttc tgatggaaaa attcataaaa actataaccg ctgggtgcgt aacctggata
     1081 aagatataca ggttaactta tctacaggag caacgttata gtgatgctga tatcgggctg
     1141 gaatggcccg atagtattta actttctcta taagaatggt tatatatgta cgaacaggac
     1201 acgctcagcg cactggatgc catcaccgaa gcacagcgga ttgcctttgc tcctatgctt
     1261 ttccaggcag ctttgtgcct gcgcaatgca ggaatattag attatttgga tcaacagggt
     1321 aaacagggag ctccccttaa tgcgattact gagcacacag tcctgaatga atatgccgtg
     1381 ggtgtgttac tggatatggg cttaagcggg cgaattatta cctgtaaaga gggaatttat
     1441 tacctggcga aaatcggtca ttacctcctg catgacacta tgactcgcgt gaatatggat
     1501 ttcacccagg atgtctgtta ccaggggctc ttcttccttg ccgattcact caatgaagga
     1561 aaaccttccg ggttaaaagt atttggcgac tggccgacga tatatccggc actttcgcaa
     1621 ttaccagacg ccgcacgtga tagctggttt gcgtttgatc attactattc agatggcgca
     1681 tttaacgccg cactgcctta tgtattcgcc aacaacccca caaccttgta cgatgtgggt
     1741 ggcaatacgg gtaagtgggc tttgcgctgc tgcaaattca atgaaaacat cgccgttacg
     1801 ttattagatc tgccacaaca aatcgtactg gcgaaagaaa atatcgcgaa tgcaggtttt
     1861 tctgatcgca tagatttcca tgcggtggat atgctaagcg atgcaccatt accaggcgag
     1921 gctgatatct ggtggatgag ccaattttta gactgttttt cacccgaaca aataattagc
     1981 atactcagca aaattgccag cgtaatgaag cctggcgcaa aactttgcat catggagttg
     2041 ttctgggatg cacagcggtt tgaagcgaca tcgtttagcc tgaatgcatc ctcactttat
     2101 tttacctgca tggcaaacgg taacagccgt ttctatagtg cagagaaatt ttacgactat
     2161 ctgaataaag caggattcca ggtagcagag cgtcacaata atttaggcgt tgggcataca
     2221 cttttgatat gccaaaagaa ataatcgtca cttaataatt tt
//
LOCUS       DAWDRC010000626         2261 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17116_length_2261_cov_2.380780, whole genome shotgun sequence.
ACCESSION   DAWDRC010000626 DAWDRC010000000
VERSION     DAWDRC010000626.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17116_length_2261_cov_2.380780"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    700..709
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          566 a          590 c          517 g          578 t
ORIGIN      
        1 caccaacggg aaagaagctg gtaacgtccg gctgctgttg atttgagttt ggggtttagc
       61 gtgacaagtt tgcgagggtg atcggagtaa tcagtgaata gctctccgcc aacaatgacg
      121 tcgtagccgt gattgcgggt tggctgccgt ccgttatccg ttccttctga ccacgccaac
      181 atatcgagga aagctttacg ctgaggatta agattttgca tttttcaccc ctgtcagtcg
      241 ttcccagaag tacgtcagtg caaccgaacc catcgcacca ctaatccccg ctgtcgcgag
      301 aatcatgtaa atactgaatc cactttcgat gctgatcagg ccaccaataa caccggtgaa
      361 tcctgatacc actatttgag ccagagcatt tatccaactc cacgttgctt tactctgctt
      421 cacatctatc aggtagcgga ccagaccgcc ccaacctgcg atgatcagca aaacgagcca
      481 gaacgctccg gcaaggctct ctttttcgtg catatgaata gccaatgttt cgccgccgac
      541 aaaaggccgg gacgttaaat gtcagaaatc aggctcacgg ggtaatttaa cgacaaagca
      601 cggagttgat gctccccgca agcctggaat aaaaaagcca gcatgtagct ggcaacagag
      661 ggttaagcaa tatcaactca acagctgaag acaccctggn nnnnnnnnnt ggctggggta
      721 ggttggaagg ctactcaccg ttcagaaaca gaaaagccca aggctttaaa cctcgggctt
      781 gaatttggat tactgccagt gcgtacaaca ttggcaaaat atcagattta cgtaaaatat
      841 atgattttta atccagtttt gcaatatctt gctgtgaaaa tatggtcttt tgttttgaac
      901 gtgttttcgt taaaagcaat aaagcctggc tatcaagctg tagaaaaatg tgcttcattg
      961 caacccagcg ttcagtaaat gtctcagacc agttttttga tgtcactccc accagtgatg
     1021 ccagctcctg gtattcatag gtcttacgcc ctgccagctc gttcttcaca tcctgtgccg
     1081 ccagccagat caacttcttc aaacgttcca gtgttttacc tgcaattttc ctggtaccca
     1141 acagagtctt aaactcgctc catgcccact gcgttatggt gacctgatgt tcccagcgaa
     1201 cactttcgct gtaactccac agcaaccacg ctttctgatg ttcttcgaga gacagaaccg
     1261 cgcggcgcca tgacgaggtt gagaactcaa cctggctgac cagtgcaatg gatgaacttt
     1321 ttgcgtacga ctgcttaccg gaaatcggcg gattatccag cgtaatcatc ctgccagtta
     1381 cctcatccag aatgcgcggc ttctttcgtt tgtatgtacc agtatcaaat tgtgcatgct
     1441 ccagccaggc ttcaagctgt cctttcgttg ctccgctcaa atcagcggta gccacaatga
     1501 gttgctcgcg gacatactgt aaatattggg tattcatgcg gcagctcctt tcagtgtttt
     1561 ggcgtaattc ttcagtatcc ggtaatcggt caaaacagaa ccaggaaaac gatataagcg
     1621 caggcgcacc cagcggcggc gaagacgctc tgccatataa gactcaaaca tcattcatct
     1681 cccagttcgg tgatagtcag ctccagcttc ccacctttgg taacgggcat cttcataaca
     1741 cggtaatcaa cgacctgaac atcatccagc cagaaacctg atttggtgag tgcgtcaaaa
     1801 gcggcttttt gcagattatc caggtcacgg cgacggcgat ccggcatgtg gcactcaata
     1861 cggattttca ctggcatagc caagccgata tccagcattg cgtttttaat gattcgggcg
     1921 acgttatcgc agtatgcctg cccctctgcg ctgacgtgcg tacgcccacg attatggcgg
     1981 taatagcgat tattgctcgg aggccagggt aatgtgatgc tgtaggtatt cacgcctcaa
     2041 taaccccctc tttcaaccag ataacctgtg ttctcgccat accttccagc gcgcattctt
     2101 ttgcatatcc agcgtcaaca aaatgcgtgc gacggtcgat ttcgtcgtgg caggcagaac
     2161 atgcaatggt ggcaatcagg tctggcggtt tgataccggt accgcacaat ccagccagcc
     2221 ggatatgtgc cagtactgac gtttcaggat tgccattaca t
//
LOCUS       DAWDRC010000627         2259 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17135_length_2259_cov_2.029492, whole genome shotgun sequence.
ACCESSION   DAWDRC010000627 DAWDRC010000000
VERSION     DAWDRC010000627.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17135_length_2259_cov_2.029492"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          501 a          650 c          545 g          563 t
ORIGIN      
        1 tgaccacgtt cttctgcgcc cacgcccgga taagcccccg gggtgtcgat aaaggtgatg
       61 ataggcatct taaaacgttc agccatttgc atcagacgca gtgctttgcg gtaaccttct
      121 ggcgctggca taccaaagtt acggcgaatt ttttctttgg tttcacgacc tttttgatga
      181 ccaatgatca tcaccggacg accatcgaga cgggcgatac caccgacgat agctttatcg
      241 tctgcatacg cgcggtcgcc agccagttcg tcaaattcat caaatgccag gcgaacgtaa
      301 tccagggtat aaggacgctg tggatggcgt gccagttgcg caatctgcca tgcaccgaga
      361 tcggcgaaga ttttacgcgt cagttctacg cttttttcac gcaaacgatg cacttcttca
      421 tcgatgttaa tatccagttt ctcatcctga cggctaaccg cggtcagaga atcgattttc
      481 gcttccagct ctgcaatcgg ctgttcaaaa tcaaggaaat tcagactcat agtattcctg
      541 tattagtcaa actccagttc cacctgctcc gaaccaatga ggccacggag atcgtttaat
      601 aaacgatcgc tcggagagac acgccacgtc gcgccaaaac gcaatcgcgc gcgtgcatcc
      661 gccctctgat agtagagatg tactggaatt gtcccagagc ggtggggttc cagagactga
      721 cggagtcggt ttaaaagctg gtcatcaatt tgcctgtccg tcagcgagat agcaagcccg
      781 cgagcatatt tttcccgggc ttcgtcaata tccatcactt cgcgagcggt cattttaagc
      841 ccaccgctga agtcatcaaa gctgacctgt ccgctgacga taagtatgcg gtctttttcc
      901 agcaattgct ggtatttatc cagggcgtca gtaaacaaca tcacttccag ccgcccggaa
      961 cggtcatcca gcgtgcagat accgatacga ttgccgcgct tggtgaccat aacccgcgcg
     1021 gcaacaacga gccccgcagc cgtgatgact ttaccacgtt ctgtcgggtg catgtctttc
     1081 agccttacgc ctccgacata acgctcaatc tcttttaaat actggttgat agggtgtccc
     1141 gtgaggtaca gacctaacgt ttcacgttcc ccatctaaca ccacctgctc cggccaaggt
     1201 tggcagctgg cgtaggattg ttcaatttgt tccggctctt cggccagcac gccgaacata
     1261 tcggcctgac cgatagcttc cgctttcgcg tgttggtctg ccgcttttaa cgcatcaccc
     1321 agcgagttca tcagcgccgc gcggtgtggc ccaagacggt caaacgcccc ggacatgatc
     1381 agtttttcca gcactcgccg gtttaacttt ttggtgtcgg tacgggcgca gagatcaaac
     1441 agttcgcgga agtagccgcc tttattacgg gcttcgatga tggcctcaat cgggccttca
     1501 cctacccctt tgatcgcgcc aataccatac acgatttcgc cgtcgtcgtt gacgtggaaa
     1561 tggtaaagac cggagtttat atctggtggc aggattttca gccccatccg ccagcactca
     1621 tccaccaggc ccaccacctt ctcggtgttg tccatatcgg cggtcattac cgccgccata
     1681 aactccgccg gatagtgcgc tttcagccat aacgtttgat atgacaccaa agcataggcc
     1741 gcagagtgcg atttgttaaa tccgtaaccc gcgaatttct ccaccaggtc gaagattttc
     1801 atcgccagtt cggcgttgat tccgttcttt tctgcacctt cagcaaatac agaacgctgc
     1861 ttagccatct cttccggctt tttcttaccc atcgcacgac gcagcatatc cgcgccaccg
     1921 agggtataac cagaaagcac ctgggcaatc tgcatgacct gttcctgata caggatgatg
     1981 ccgtaggttg gctccagtac cggtttcagg ctttcatgct gccactgcac gtccggatag
     2041 gagatctctt cgcgaccatg tttacggtcg ataaagttat ccaccatccc tgattgcaac
     2101 ggaccggggc ggaaaagtgc caccagtgcg atcatatctt cgaagcagtc aggttgcaga
     2161 cgcttgatca ggtctttcat gccgcgcgat tcaagctgga ataccgccgt ggtttccgag
     2221 cgttgcagca tgtcgaagcc tttcttgtca tccagcggg
//
LOCUS       DAWDRC010000628         2252 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17205_length_2252_cov_1.661812, whole genome shotgun sequence.
ACCESSION   DAWDRC010000628 DAWDRC010000000
VERSION     DAWDRC010000628.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17205_length_2252_cov_1.661812"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1960..1969
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          575 a          527 c          588 g          552 t
ORIGIN      
        1 aggcgcatcg ggtcaacaca tatgcccgat gcgtcagttt atcgggcctg gaggaaagcc
       61 acatggcata cacccgcagg cccgcatccg gcaagttaca acaaacaacc tttaaccatg
      121 ctttttgatg tttttcagca ataccccgcg gcgatgccca tactggcaac cgtcggagga
      181 ttgatcatcg gcagtttttt gaatgtggtg atttggcgtt accccatcat gctgcgccaa
      241 caaatggcgg agtttcatgg tgaaacgccg agtacgcagt caaaaataag ccttgcgcta
      301 ccgcgttcgc actgtccgca ttgtcagcag accatccgcg ttcgtgacaa tattccgctg
      361 ctctcctggt tgatgctcaa agggcgctgc cgtgactgtc aggcgaaaat cagcaagcgt
      421 tatccgctgg tggaattatt gacagcactc gcttttttgc tggcgagtct ggtgtggcca
      481 gaaagtggat ggggactggc ggtgatgata ttatccgcgt ggctgattgc cgcgagcatc
      541 atcgacctcg acaaccaatg gctgcccgat gtttttactc agggcgtatt gtggaccggg
      601 ctgattgcgg catgggcgca acagagtccg ttaacgctac aagacgcagt caccggcgtc
      661 ctggtggggt ttatcacttt ttactccctg cgctggatag ccggaatagt tctgcgtaaa
      721 gaagcattag gcatgggcga tgtattactt ttcgctgcgt taggtggctg ggtggggccg
      781 ttgtcgctac ccaatgtggc tttaatcgcc tcatgctgcg gcctgatata tgccgttatt
      841 acaaaaagag gatcaaccac actgcctttt ggaccgtgtt taagtctggg cggtatggca
      901 acactttatc tacaggcatt gttttaatga taaccacgtc attatcaaag tgacatttta
      961 actcttatta ataaccttag agattattta ccatgtcgat aaaacagatg ccagggaggg
     1021 tattaatatc gctattgttg agcgttacag gattattaag tggctgcgcc agccataatg
     1081 aaaacgccag tttactggcg aaaaaacagg cgcaaaatat cagccaaaac ctgccgatta
     1141 aatctgcggg atatacctta gtgctggcgc aaagtagtgg gacgacggta aaaatgacca
     1201 ttatcagcga atcgggtact cagaccacgc agacacctga cgccttttta accagctatc
     1261 aacgacaaat gtgcgctgac ccaacggtga aattaatgat caccgaggga attaattaca
     1321 gcataacgat taatgataca cgtacaggta accagtatca gcggaaactg gatcgtacca
     1381 cctgtggaat agtcaaagca taacgtcggg tagatataaa ttggcgcggg ttgtttttcg
     1441 tgacgcacga atttatctca ttcaatggct gactaaaatt cgtcacactc ttagccagag
     1501 acaatctctt aatacagaca aagagcatct gcgcaaaatt gcacgcgcga tgttctggct
     1561 gatgctgctt attatttctg caaaaatggc gtattcactc tggcgctatt tctccttttc
     1621 tgcggaatat acggcggttt cctcatcggt gaataaaccg ctccgtgcgg atgcaaaacc
     1681 gttcgataaa aatgacgtgc aattagtcag ccaacaaaac tggtttggca aatatcaacc
     1741 cgtcgccgca ccggtaaaac aacctgaatc tgcgcctgtg gcagaaacgc gtcttaatgt
     1801 ggtgctgcgt gggatcgcct ttggtgccag acccggcgcg gtgattgaag aaggcggcaa
     1861 acagcaggtc tatttgcagg gtgaacggct tggctctcac aacgcagtga ttgaggaaat
     1921 taatcgtgac catgtgatgc tgcgctatcg ggggaaagtn nnnnnnnnna atggaacgtc
     1981 tgagtctggc agaggaggaa cgcccccctg tggcggtgac cagcaaaaaa gccgccagtg
     2041 acgaagcaaa gcaagctgtt gctgaacctg tcgtcagtgc gccagtggag atcccggctg
     2101 ccgtgcgtca ggcactggcg aaagatccgc agaaaatttt taactatatc cagcttacgc
     2161 ctgtgcgtaa ggaggggatt gtcggttatg cagtgaaacc gggggcagat cgttctctgt
     2221 tcgatgccag cggttttaag gaaggcgata tc
//
LOCUS       DAWDRC010000629         2251 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17211_length_2251_cov_2.204463, whole genome shotgun sequence.
ACCESSION   DAWDRC010000629 DAWDRC010000000
VERSION     DAWDRC010000629.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17211_length_2251_cov_2.204463"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          461 a          609 c          659 g          522 t
ORIGIN      
        1 tccgcgcggt acagacagcc ttgcagtcgc ccggcgaggg cttccagcgg cgcggggttg
       61 agactgtcgg taaacacctg ggcgcgggtg atatggcctt tttcaacgtc gaaatgcagt
      121 tccacgccgc cccaggtaaa gcgttcatcc agcagatgcg agaatgccgg agcctgaccg
      181 aagttccatt cccagctact ctggcgggca aaggtttcgg cgaagtttgg caagtctggc
      241 gttttgtccg gggagatgat ttccgcttcc acgcgctcgc cataatgggc gaaaaaggcc
      301 tcggttatgg cctcgcaaac ctgctcatgg gtgatccccg gtagcaattc ggtcagattg
      361 gtcacgcggg aacgcactga ggtaatgcct ttcgccgcca gtttcttttt atccggattg
      421 agatagtttg ccaggcggct aaggtcggaa ttgagcagca aagtgccgtg gtggaagcca
      481 cgatctttgg tttcgcgata ggccgagcct gagactttgc gatcgccttc ggcggttttc
      541 accaccagat cgttacgccc ggaagcttcg gcgctgacgc cgagcgcgtt cagcgcattg
      601 agcacaatcg acgtggagat ggttttatcg tactccggct tgccagccat aaaggtaaaa
      661 caggtattgc cgagatcgtg gaacactgcg ccgccaccgc tactgcgccg cgccaggcgg
      721 acgttatctt cttccatccg ccgggtatta cactctttcc acgggttctg cgcgcgacca
      781 atcactaccg tgtcggcatt gcgccagaga aacagaacgc gctgcgtagc cggcatctgg
      841 cgaaaaatac actcctctac cgccaggtta aaccacgggt cgtaagagtc agagatgagc
      901 aggcgtaatg tggacataac gatttccttt cttgtagtaa tgggtcactc ttttttctcg
      961 ctttcttcct cttccggcac cggtttactg gcggttaaca ggaagggcga ttgctgccag
     1021 cgggggcgtt taccctgtag cagcgtgcgg gtcagcacta cgccgattgc cagcgagagc
     1081 agcaacatca ggcgtaaaat gttagtggtg ttatccacct gttgggcttc ggtggcgagc
     1141 gtatgggtgt cgagcgtcag gcgcagatag ccgagcggtc cgtttttacc cgcaatgggt
     1201 tcgacaatct gctggttaaa atagccgccc gcttttttgc cgtcgagcgc cagccggtcg
     1261 cgcacgtcga cgctttcgcc agaacgggcg ataagatcgc cttgctcatc atatacgcct
     1321 gcgtcgagaa tgcggctttc atccgttaac tgatcgagga tcgcctgaat gcgtttttca
     1381 tccggtgagt cggtacgcat cagcggtgca acgtttagcg tcacctgccg cgccagggtg
     1441 cgggccagtt cttccagttg cggattacgc tgtcgctggt ggttttgact aaaccatgac
     1501 gccccctgca tcagcgccac taacaaggca agacagaaca ggacaatcac tgcccgatgc
     1561 agccggaatt tcagttttgt gcgagccatc ttccaccctt tgaaaatttg agacttaatg
     1621 ttgccagaag caatggatac aaggtagcct catgcgttat tttccctgct tcgaacgatt
     1681 ttacaggagc cttaatgcct aacattacct ggtgcgacct gcctgaagat gtctctttat
     1741 ggcctggtct gcctctttca ttaagtggtg atgaagtgat gccactggat taccacgcag
     1801 gtcgtagcgg ctggctgctg tatggtcgtg ggctggataa acaacgtctg acccaatacc
     1861 agagcaaact gggcgcggct atggtgattg ttgccgcctg gtgcgtggaa gattatcagg
     1921 tgattcgtct ggcaggttca ctcactgcgc gggctacgcg cctggcccat gaagcgcaac
     1981 tggatgtcgc gccgctgggg aaaatcccac acctgcgcac gccggggttg ctggtgatgg
     2041 acatggattc caccgccatc cagattgaat gtattgatga aattgccaaa ctggccggaa
     2101 cgggcgagag ggtggcggag gtaaccgaac gggcgatgcg cggcgaactc gattttaccg
     2161 ccagcctgcg cagccgcgtg gcgacgctga aaggcgctga tgccaatatt ctgcaacagg
     2221 tgcgtgaaaa tctgccgctg atgccaggct t
//
LOCUS       DAWDRC010000630         2247 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17251_length_2247_cov_2.142792, whole genome shotgun sequence.
ACCESSION   DAWDRC010000630 DAWDRC010000000
VERSION     DAWDRC010000630.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17251_length_2247_cov_2.142792"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1187..1196
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          600 a          517 c          592 g          528 t
ORIGIN      
        1 gcagctccct gtggccgaaa ggcattaaaa gcgtgactgg caatttctcg cgtggtgaag
       61 tcatccgtat ttgcaacctc gaaggtcgcg atatcgccca cggcgtcagt cgttacaaca
      121 gcgatgcatt acgccgtatt gccggacacc actcgcaaga aattgatgca atactgggat
      181 atgaatacgg cccggttgcc gttcaccgtg atgacatgat tacccgttaa ggagctggcc
      241 gatgctggaa caaatgggca ttgccgcgaa gcaagcctcg tataaattag cgcaactctc
      301 cagccgcgaa aaaaatcgtg tgctggaaaa aatcgccgat gaactggaag cacaaagcga
      361 aagtatcctc aacgctaacg cgcaggatgt tgcagacgcg cgtgccaatg gccttagcga
      421 agcgatgctt gaccgtctgg cactgacgcc cgcacggctg aaagacattg ccgacgatgt
      481 gcgccaggtg tgcaatctcg ccgatccggt ggggcaggta atcgatggcg gcgtactgga
      541 cagcggcctg cgtcttgagc gtcgtcgcgt accgctgggg gtgattggcg tgatttatga
      601 agcgcgcccg aacgtgacgg ttgatgttgc ttcgctgtgc ctgaaaacag gtaacgcggt
      661 gatcctgcgc ggcggcaaag aaacctgtcg cactaacgct gcaacggtag cggtgattca
      721 ggacgccctg aaatcctgcg gcttaccggc gggtgccgtg caggcaattg ataatcctga
      781 ccgtgcgctg gtcagtgaaa tgctgcgtat ggataaatac atcgacatgc tgatcccgcg
      841 cggtggggct ggtttgcata aactgtgccg cgagcagtcg acaatcccgg tgatcacagg
      901 cggtataggc gtatgccata tttacgttga tgaaagcgca gagatcgctg aagcattaaa
      961 agtgatcgtc aacgcgaaaa ctcagcgacc gagcacatgt aatacggtag aaacgttgct
     1021 ggtaaataaa aacatcgcag atagcttcct gcccgcatta agcaaacaaa tggcggaaag
     1081 cggcgtgacg ttacacgctg atgcagccgc gctggcgcag ttgcaggcag gccccgcgaa
     1141 ggtggtggct gttaaagccg aagagtatga cgatgaattc ctgtcannnn nnnnnntacg
     1201 ttaacgcctc tacgcgtttt accgacggcg gccagtttgg tctgggtgcg gaagtggcgg
     1261 taagcacaca aaaactccac gcgcgtggcc caatggggct ggaagcactg accacttaca
     1321 agtggatcgg cattggtgat tacaccattc gtgcgtaaat aaaaccgggt gatgcaaaag
     1381 tagccatttg attcacaagg ccattgacgc atcgcccggt tagttttaac cttgtccacc
     1441 gtgattcacg ttcgtgaaca tgtcctttca gggccgatat agctcagttg gtagagcagc
     1501 gcattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt ctaacgtctc
     1561 cccaagtcta ctcaagtatt taaaaacctc ttataatcag tatattaatg ccccttttag
     1621 tcttttgacg tctatttaag taccccaaac tctacaagca attgagggga tttgctgttc
     1681 agtttagtgg agataccccc aagtgaaact caatgcccgt caaatagaca ctgccaagcc
     1741 aaaagagaag gcttacaagt tggctgatgg tggtggtctg tacctcctgg taaaacctgg
     1801 tggaggagaa tattggcgtc tcaagtatcg tgtagctggt aaagagaagc tgttagcact
     1861 aggtgtgtat cctgaggtca ccttagctga tgctcctgca aaacttgaag aagctaaaag
     1921 aggtatctct gggggaatcg atctgatgga agtgaagcga gaggaaaaga ttgcccggga
     1981 aacgcagtta aacaacacct tcaaagatat tgcccttgag tggcacagta acaaattata
     2041 atcacaataa tagtgtatat aattatacat tgtatgcttg gtaatcatat aatgataaaa
     2101 gcttccgata atcagtatga gatttgtcct taaactcctg gacatgcttt tctaaatctt
     2161 ccaatggtat atgtccattt atacaaataa aattctagtg ataaatacct tcaggtatga
     2221 ttagacaaca tctttattcg atggggg
//
LOCUS       DAWDRC010000631         2246 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17260_length_2246_cov_1.580100, whole genome shotgun sequence.
ACCESSION   DAWDRC010000631 DAWDRC010000000
VERSION     DAWDRC010000631.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17260_length_2246_cov_1.580100"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          509 a          573 c          678 g          486 t
ORIGIN      
        1 aaaggcgctg atgccaatat tctgcaacag gtgcgtgaaa atctgccgct gatgtcaggc
       61 ttaacgcagc tggtactcaa gctggaaacg ctgggctgga aagtggcgat tgcttccggc
      121 ggctttactt tctttgctga atatctgcgc gacaagctgc gcctgacagc cgtggtagcc
      181 aatgaactgg agatcatgga tggtaaattt accggcaatg tgatcggcga catcgttgac
      241 gcgcagtaca aagcaaaaac tctgacccgc ctcgggcaag agtatgaaat cccgctggcg
      301 cagaccgtgg cgattggcga tggagccaat gacctgccga tgatcaaagc ggcagggctg
      361 gggattgcct accatgccaa gccaaaagtg aatgaaaaaa cggaagtcac catccgtcac
      421 gctgacctga tgggggtatt ctgcatcctc tctggcagcc tgaatcagaa gtaattgcct
      481 gcccgtcatc ctgcgggcgg cacagcatta acgaggtaca ccgtggcaaa agctccaaaa
      541 cgcgcctttg tttgtaatga atgcggggcc gattatccgc gctggcaggg gcagtgcagt
      601 gcctgtcatg cctggaacac catcaccgag gtgcgtcttg ctgcgtcgcc aacggtggcg
      661 cgtaacgagc gtctcagtgg ctatgccggt agcgccggag tggcaaaagt ccagaaactc
      721 tccgacatca gccttgagga gctgccgcgt ttttctaccg gatttaaaga gttcgaccgc
      781 gtattaggcg gcggggtggt gccgggaagc gccattctga ttggcggtaa cccaggcgcg
      841 gggaaatcca cgctgctgtt gcaaacgctg tgcaaactgg cccagcagat gaaaacgctg
      901 tatgtcaccg gcgaagagtc gctgcaacag gtggcaatgc gcgctcatcg ccttggcctg
      961 ccgactgaca atctcaatat gttgtcggaa accagcatcg agcagatctg cctgattgcc
     1021 gaagaagagc aaccgaagct gatggtaatt gactccatcc aggtgatgca tatggcggat
     1081 gttcagtcat cgcctggcag cgtggcgcag gtgcgtgaaa cggcggctta tctgacgcgc
     1141 ttcgccaaaa cgcgcggcgt ggcgattgtc atggttgggc acgtaaccaa agatggctcg
     1201 ctggctggcc ctaaagtgct ggaacactgt atcgactgtt cggtgcttct ggatggcgat
     1261 gccgactccc gttttcgcac cttacgcagc cataaaaacc gcttcggcgc ggtgaatgag
     1321 ctgggtgtct ttgcaatgac cgagcagggg ctgcgtgaag tcagcaaccc ttccgccatc
     1381 tttttaagcc gcggtgatga agtgacctcc ggtagctcag tgatggtggt atgggaagga
     1441 acgcgtccac tgctggtgga gatccaggcg ctggtcgatc actcgatgat ggcgaatcca
     1501 cgccgcgtgg cagtcgggct ggagcaaaac cgtctggcaa tcctgctggc ggtgctgcac
     1561 cgtcacggtg ggttgcaaat ggccgatcag gatgtatttg ttaacgtggt cggcggcgtg
     1621 aaggtgacgg aaaccagcgc cgacttagcg ttactgctgg cgatggtttc cagcctgcgc
     1681 gacagaccgc tgacacagga tctggtggtg tttggcgaag tcgggctggc gattgttccg
     1741 gcggctaacg tgccgaaaaa agcgccggaa gggatgcaga tttttggcgt taaaaaactc
     1801 tccgacgcgc ttagcgtgtt cgacgactta taatgagaga tacggaggga gatatgtcgt
     1861 catttgatta cctgaaaact gccattaagc aacagggctg cacgctacag caggtagctg
     1921 atgccagcgg tatgaccaaa gggtatttaa gccagttact gaatgccaaa attaaaagcc
     1981 ccagcgcgca aaaactggag gcattgcacc gttttttggg gcttgagttt ccccggcaga
     2041 agaaaacgat cggtgtcgta ttcggtaagt tttacccgct gcatactgga catatctacc
     2101 ttatccagcg cgcctgtagc caggttgacg agctgcatat cattatgggt tttgacgata
     2161 cccgcgaccg cgcattgttc gaagacagcg ccatgtcgca gcagccaacc gtgccggatc
     2221 gtctgcgctg gttattgcag accttt
//
LOCUS       DAWDRC010000632         2238 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17326_length_2238_cov_2.480989, whole genome shotgun sequence.
ACCESSION   DAWDRC010000632 DAWDRC010000000
VERSION     DAWDRC010000632.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17326_length_2238_cov_2.480989"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          615 a          580 c          524 g          519 t
ORIGIN      
        1 gaaaatcctt acataaaaca attgaacgaa atcaggtaaa aacagcaaaa aaattgcctg
       61 gaccagttcc ggcgctggta attacagcgt ttgtcgcccg ccgcttactg cgctttcgtc
      121 atatgcttgc ctgtcgtcgt cgcgggttaa ttgttttaac cgaccgttat ccgcaggacc
      181 agattcctgg cgcttatgat ggtacggtgt ttccgcctaa cgttgaaggt ggtcgttttg
      241 tctcatggct ggcaagtcag gaacgtaaag catttcactg gatggcgagc cacaagcccg
      301 atctggtcat caaacttaat gttgaccttg aagttgcctg cgcgcgtaaa cccgaccata
      361 aacgggaatc gctggcgagg aagattgcca taacgccaca gttaaccttt ggcggtgcac
      421 aactggttga tatcgatgct aatcagccac tggaaaaggt gctgattgat gcagaaaaag
      481 cgattacgga ttttatgacc gcgcgtggtt atcactagtg aaaaatggaa atgcccgatc
      541 gccaggaccg ggcattttca ggaaggttaa atcaactgca atgctatcca gtacaaccca
      601 ccagaaagaa aaatcgccgc cggtaaagta aacacccacg ccatcaggat gctggttacc
      661 gttttacgct gtaacccacc gccgtccacc accatcgtcc ctgctactgc agacgagagt
      721 acgtgtgttg tggagacggg catcccaata taactggcaa ggccgataga cactgccgcc
      781 gtcatttgtg ccgccatgcc ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg
      841 atggtcatcg ctacacgacg ccagccaatc atggtgccga tgccgagcgc cagtgctacc
      901 gccatgatga tccataccgg tgcgtactca atggtgctta acatatcgct gcgcagtttt
      961 ttcagcaggt tctggtcttc tttactgacg cctggcagtt tcgccagctt cgcggaggta
     1021 tcagagatgc acagcataat gcggcgcagc tggctgcgct gactcacgct taacggctcg
     1081 taactttcca tattgcctgg cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt
     1141 gccggatgac agtgaaactc tgttacttgc gtgccatcag tcgatgtcgc aggcaatgga
     1201 ggttccatcg taatcaactt ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag
     1261 ttggtgacgg catcgcgggt acgggtaatt tcatagccgg acgcgttcat attgacgaca
     1321 aacccggcgg gagcaatccc caccagcacc agcatcacca gaccaattcc tttctgcccg
     1381 tcattcgcgc catgtgaaaa cgccacgccc gcagcggaaa caatgagggc aatacgcgtc
     1441 cagaatggcg gcttacgttt gccttttttc tttttgcgat cttcaggaat acgatgaata
     1501 cggtcacgct ttttcgtccc actccagaaa cggcgtagca gaaaaattag ccccccgcaa
     1561 tcaccagccc cacgatggga gagacaatga gtgaagagaa aatcttggtg acttcgcgca
     1621 ggtttaacgc atccatcacc gacgaaccat ttaacagcgc gttggttaaa ccaatgccga
     1681 taatcgcacc aatcaaagtg tgcgaactgg aggccggtaa accaaagaac cacgttccca
     1741 ggttccagat aatcgccgcc agcagcatgg aaaagaccat cgccagaccg tgagttgacc
     1801 ccatattcag caacaaatcg gttggcaaca tatggacgat ggcataggca acgctaagcc
     1861 cgccaagcaa tacgccaaaa aagttaaaaa atgccgccat gaccacagca agctgtggtt
     1921 gcatggcacg ggtataaata acgtttgcca tcgcattcgc cgtgtcatga aaaccattga
     1981 ttgcttcata gaacaacaca aatgccagag caagtaataa caaaagcccc gtatatatat
     2041 caaggccaac aaataaattt agcatatatt agattacgcc attttgaatt tacgaacgga
     2101 ggcattatca gtgactttca cggcgtgggc aaagtgaaat atcatttttt aacgataaaa
     2161 agcgtgttat ttcttgtgta tcagacaata agccttcatt caaaaccttt aaaaataatc
     2221 aaaacacagg tattccac
//
LOCUS       DAWDRC010000633         2235 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17355_length_2235_cov_3.240826, whole genome shotgun sequence.
ACCESSION   DAWDRC010000633 DAWDRC010000000
VERSION     DAWDRC010000633.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17355_length_2235_cov_3.240826"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          548 a          518 c          543 g          626 t
ORIGIN      
        1 cattcagctc gttaataaga gagttaactt attaagcgtt agcgtttatt actgaggtaa
       61 caccatgaaa agattaattc ctgtgacatt gctcaccgca ttgctggcgg gctgtgctca
      121 cgattcgccc tgcgttccgg tttatgacga tcagggtcgt ctggttcaca ctaatacctg
      181 tatgaaaggc acgactcagg ataactggga aacggctggg gctattgccg gcggggcagc
      241 agctgttgct ggcctgacga tggggatcat cgctctgtcg aaataactaa acagggcgcg
      301 gagctccgcg ctctttttaa cgatacagta tctttccata agataaaata ttgctatatt
      361 ctgctgggta attccctgct gatttagcat gttatatcac gttatttcgc tgctttcgaa
      421 ataacgcatc aatttaactt tcacacatcc caccagaaat atactcacgc taacaatcat
      481 tattctgctt agtaaaaaac gcgtaaatat tttgcgccaa aatgtggcgc atgtttcatt
      541 ttcgcaccat tgcggggcgc tgtttttatt atttcctgtc tacactcagc ttattgcgac
      601 gcgaaatcgt gcgcaaaagc tggcactact tttgcttata agaggatggc cacagacagg
      661 taaaagacgt ttcccaaacg tcctataacg ataattttcg cctcacagga agcattatga
      721 aaaagatgat gatagccaca ctggctgccg ccagcgtgct gcttgccgtt gcaaatcagg
      781 cgcatgctgg cgcgacgctt gatgccgttc agaaaaaagg ttttgtgcaa tgcgggatca
      841 gtgatggatt acctgggttc tcttatgccg atgctgacgg taagttttca ggtattgatg
      901 tagatgtttg tcgtggtgtt gccgctgctg tattcggtga cgatacgaaa gtgaaatata
      961 ccccgctcac tgcaaaagaa cgcttcaccg ctttacagtc aggggaggtg gatttgctct
     1021 cccgtaatac gacctggact tcatctcgcg atgccgggat gggaatggca tttactggcg
     1081 tcacttatta cgacggcatt ggcttcctga cgcacgataa agcggggcta aaaagcgcga
     1141 aagaactgga tggcgctacc gtctgtattc aggcggggac tgataccgaa ctcaacgtcg
     1201 ccgattactt caaggcaaat aatatgaagt acacgccagt aaccttcgat cgctcagacg
     1261 aatcagcgaa agctctggaa tcaggccgct gtgatactct ggcctcggat caatcacaac
     1321 tgtatgccct acgcatcaaa ttaagcaacc cagctgaatg gattgtctta ccggaagtta
     1381 tctctaaaga accacttggg ccggtagttc gtcgtggcga tgatgaatgg ttctcgattg
     1441 tacgctggac gcttttcgcc atgctgaatg ctgaagagat gggcatcaat tcccagaacg
     1501 tcgatgaaaa agcggctaat ccagcaacgc ctgatatggc acatctgctg ggtaaagaag
     1561 gcgattacgg caaggatctg aagctggata ataaatgggc ctttaacatc atcaaacagg
     1621 tgggtaacta ctcggaaatt ttcgagcgta acgtaggttc agaaagcccg ctgaaaatta
     1681 aacgtgggca aaataatctc tggaataacg gcggtattca gtacgcgccg cccgtgcgtt
     1741 aagtcggtgt gaggtaacga gcgtcgctac ggcggcgctc tcttcagcgt tatgctttcc
     1801 gaggtttcta tgtctcatcg ccgctcaacc gttaaaggct cactctcttt tgccaaccct
     1861 acggttcgcg cctggttatt ccagatcctt gccgttgttg ctgttgtcgg cattattggt
     1921 tggttatttc acaacactgt aacgaatctc agtaatcgcg gcataacatc aggatttgct
     1981 tttcttgacg cggggccggt tttggcatcg ttcagcattt gattgattat cagcaaggcg
     2041 acacttacgg acgcgttttt attgtcggcc tgctcaatac gctactggtt tctgcattgt
     2101 gtattgtgtt cgcttctgtt ttgggcttct ttatcggtct ggcgagactt tcggataact
     2161 ggctgctacg aaagctttcc acaatttata ttgagatctt ccgtaatatt ccgccattgc
     2221 tgcaaatctt cttct
//
LOCUS       DAWDRC010000634         2230 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17406_length_2230_cov_2.352184, whole genome shotgun sequence.
ACCESSION   DAWDRC010000634 DAWDRC010000000
VERSION     DAWDRC010000634.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17406_length_2230_cov_2.352184"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          608 a          503 c          511 g          608 t
ORIGIN      
        1 aaggttcatc actgtttcat tcaccagata tgggttaatc ttttaattac aaactgcgtt
       61 gacagttgtt gtaaggagac cctgtatggc tatgtatcag aacatgctcg ttgttatcga
      121 tcctaaccag gacgaccaac cagcattgcg gcgagctgtt tatttacatc aacggattgg
      181 tggcaaaatt aaagcctttt tgccgatcta tgacttctca tacgaaatga ccaccctgct
      241 ctctccggac gaacgtaccg ctatgcgtca gggcgtcatc agccagcgta cagcctggat
      301 ccacgagcag gcaaaatatt atctcaatgc tggcgttccc attgaaatta aagtggtctg
      361 gcataaccgt cctttcgaag ccatcattca ggaagtgatc agcggcgggc acgatttggt
      421 gctaaaaatg gcgcaccaac atgaccgtct ggaagcggtg atttttacgc caacggactg
      481 gcatctgtta cgcaaatgcc caagcccggt gtggatggtg aaagaccagc cgtggccgga
      541 agggggtaag gcgctggtgg cggtgaatct cgccagtgaa gagccgtacc ataatgcgct
      601 caatgaaaaa ctggtcaaag agacgatcga actggcagaa caagtcaacc ataccgaagt
      661 tcatctggtt ggcgcttatc cggtaacgcc aattaatatc gcgattgaac tgccggaatt
      721 tgacccgagc gtttataacg atgccattcg tgggcaacat ttgctggcaa tgaaagctct
      781 gcggcagaaa ttcggcatta atgaaaacat gacgcacgta gaaaaaggtc tgccagaaga
      841 ggtgattcct gatttggcgg agcacttaca ggcggggatt gtggttctgg gcacggtcgg
      901 acgcaccggt atttcagcag cattcctcgg caacacggcg gaacaggtga ttgatcatct
      961 tcgctgcgac ctgctggtca ttaaacctga ccagtatcag acacccgttg aactggatga
     1021 cgaagaagac gattaacgac tcttcaataa ctgacaaggc ccgagttatc gggcctattt
     1081 tttacttact gctcttcgcc gccaaggaaa taaattccca acgggattgc cagcaacaca
     1141 gtaaagacca ggctataaac aaaaatcatc gatgactgaa tcacatacat tgatgtcgtc
     1201 cactcagaca gcggaatatt gtactgttct ataacgccgc cgatggtcgc gcgagtcaca
     1261 accgacgccg caataatgaa aaccgccaac aaaccaaaga gaaatttctt ccctttcgct
     1321 gacttcagtt tcgccataat catgatgtaa tccttgtgca gacaaatttc ttgttaccta
     1381 aacgtaacaa taccatgaca tttcaccgtt gtctgctctg aaaatctcac ctcttagctg
     1441 gatttacaat caacgcattg attagttagc taatgttgct acttccttcc ctgactattg
     1501 atgatctgaa tgaattgtcg cggtaaaata gtcccgccag gggtttccgg catgacgaca
     1561 attatctgga ggagcctgat aaaacaatga tcgctgaact gtttacaaat aatgcgctta
     1621 atctggtcat tattttcggt agctgcgcag cattgattct gatgagcttt tggtttcgcc
     1681 gtggagatcg taaaagaaaa ggatttttat tccatgcggt gcaattttta atctacacca
     1741 taattattag tgctgttggt agcatcatta actatgttat agaaaactat aaactcaaat
     1801 ttatcacccc aggcgttatc gattttatct gtacgtccct gattgcggtt attctgacga
     1861 ttaagctatt tctgctgatt aatcagtttg aaaaacagca gattaaaaaa ggtcgcgata
     1921 tcaccagtgc gcggatcatg tcgcgcatta tcaaaatcac cattattgtg gtgcttgttc
     1981 tgctttatgg cgaacatttc ggcatgagcc tttctggctt gttgaccttt ggtggtattg
     2041 gtggtctggc tgtcggtatg gccggtaaag atattctgag taacttcttt tccgggatta
     2101 tgctctattt cgaccgtcct ttcagtattg gcgactggat ccgttcaccg gacagaaata
     2161 tcgaaggtac agtaacggaa attggctggc gaattaccaa aattaagacc tttgataatc
     2221 gtccattgta
//
LOCUS       DAWDRC010000635         2230 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17411_length_2230_cov_1.754943, whole genome shotgun sequence.
ACCESSION   DAWDRC010000635 DAWDRC010000000
VERSION     DAWDRC010000635.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17411_length_2230_cov_1.754943"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1310..1319
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          540 a          594 c          538 g          548 t
ORIGIN      
        1 gcgcgattgt tggtcaatgc aaccagccac aaaaaggcct tactttcgac cagtttctga
       61 ttaaagaaat aaaaaatatc cgcatcaatc ggcaaccaga atccatgatt aatgggaata
      121 taccaggaaa gaaacagcgc gaggccgaca atattcaaca acactatttg cggcaaattt
      181 ttaatcatat tttctctaat tatcttactg aaaaacgcag gcaaccttac gcggtagtcg
      241 ctaaacgaag cttcaacagc gcgctctgca aggcattcca gtcagcttcg ctgctggaaa
      301 tcagctcaat acgactgtcc ggtggcgcaa cgttttgcgt ttcaatgtgc aggtcatcgc
      361 cctgacggtt gattcgcacc agcccttcgg gaatacgcag cacgcctttg acgcgttcca
      421 ccggtgcaag tcgcgcccat tccagaatac caatggtgtc gaataccgta tcagcgtcga
      481 atatccagcc acaggcctga tgcccttgtc cgctgttcag actgcgacgc cagcgttgat
      541 gctctggcag gcttaacgct gctaaccctt ttttcacgct atgctgatga gaatgcgcgg
      601 cgctggcggg caactcggct aaattgcgac gcggcaaatc cagaagatga ccgtcaattt
      661 ttccatgctc gctgtgaatt aattgccgat cgccaccatt ttgctgccac cagcgctcta
      721 gcgcttgctc actttcgggc gtcgcacggt cggatttatt ggcgacaata atgtctgcgg
      781 cagccagttg gtcacggaag ttttcattgc tggcgctttt ttcgtccagc aacaggcgtg
      841 gatcgagaat gcacaacgtg gcgcgcagat ctatccacgg ttcatagacg ggtgcggtta
      901 agagatcgag gatctgtttc ggatggccca gcccggtcgg ctctatcaac aagcggtctg
      961 gttttccctg acgcagtaag gtattcaacc ctacctgcat gggtaaacca ttaacgcagc
     1021 acatgcaacc gccggggatc tctttcagca gtgcgccgct atcggcgagc aaagcaccat
     1081 caattccgac ttccccaaat tcattaacca gtaccgccca tttttcgttg ggatctttat
     1141 gggctaacag atgaagaatc gacgtagttt tcccgctgcc gagaaaaccg gtgatgaggt
     1201 tggttctggt cacaacggct ccgcaatact ctggtaagtt aactgagcgt taatcctggc
     1261 taaaaaaggc agaaaagaga aggggagagc ggacgaaggc gagcttcatn nnnnnnnnnt
     1321 tcatccgctt taaccgcaga aatcgttaaa ttttgagggt gtttaatagc gcctgatgcg
     1381 cgccgaattg tgcgatttgt tgccaggctt gttcgatggc ctgcacaaaa tgcgggttat
     1441 ctggcagatc atcaccgaaa atttcacgca gggaaagcag ggcggttacg cgttgttcac
     1501 tgctgctgcc cgcaacaagt tcacgaattt tatcgctaag tggatcgcga acatcaatgg
     1561 catttccggc atcatcaacg ccgctgacgt aacgcatcca gcctgcaacg cctaatgcca
     1621 gcaacgacca gtccgtttcg cgcctcagat gtatgcgaat acctgccaac atgcgttgcg
     1681 gtaatttctg gctgccatcc atcgcgattt gcctggtctt atgtttcagt gccggattag
     1741 caaaacgtgc aattaactta tccgcatatt gtgttaaatc gacatcttta atccgcagtg
     1801 tcggcgcttg ctcatccagc attaatgttc tggcggcatg gcgaaatgcg cgatcctgca
     1861 tgcaatcact gatatgggcg aatcctgaga ggtaacccag ataagcgaga aaagagtggc
     1921 tgccattaag catccgcagt ttcatctctt cccatggcag gacatcattc accatttgta
     1981 cacctgcgat ttcccaggca ggacgcccag cgacgaagtt atcttcaacc acccactgga
     2041 taaacggttc gcagctaatc gcgcagggat cattcacccc cagatgctgg ctgatttccg
     2101 ccagtgattc gtttgttgca gccggaacaa tgcggtcgac catagttccc ggaaaactga
     2161 cgtgttcttt tatccacccg gcgagttctg gcgaacgttt ttctgccatt cccagcaccg
     2221 cgtttttcac
//
LOCUS       DAWDRC010000636         2210 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17565_length_2210_cov_1.697912, whole genome shotgun sequence.
ACCESSION   DAWDRC010000636 DAWDRC010000000
VERSION     DAWDRC010000636.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17565_length_2210_cov_1.697912"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    317..326
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    588..597
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          505 a          617 c          557 g          511 t
ORIGIN      
        1 ccgggcgacc cagaataccg gtcggcatca ccagcagcac caggatcagc agggcgaatg
       61 agaccacatc tttatattcc gtactcagat aggcagaaga gagcgcctcc gcaatcccca
      121 ggatcagccc gccaatcatc gcccccggaa tgctgccaat cccaccgagc accgcagcgg
      181 taaaggcttt catcccggcc ataaagccga tgtaggggtt aattacgccg tagaactgac
      241 cgagcagcac acccgccacc gccgccatcg ccgcgccaat cacaaaggtc agcgcaatca
      301 cccgatcggt gttgatnnnn nnnnnntttc agatcttccg cgcaggcacg acaggcgcgc
      361 cccatgcggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa agtaacaatc
      421 catatcaccg cctgcatggt ggtaatagac gcagagaagt tttcgctatg gcccaccacc
      481 cactgaccgt taaacaggct gggcagcgcc acatcgcgcg aaccttcggt caggctgacg
      541 tagttttgca ggaagataga catgccgatt gccgagatga gtgcaatnnn nnnnnnncgc
      601 accgggcgat aggctacccg ttcgatactc cagccgtagg cgctggcaat gacgattgcg
      661 ccgacgaatc ccgccgccac cagcagccag ccggtatcaa tgcccagcat catcagtgcg
      721 gcgatgatca taaaggagac gtagctgcca atcatgtaaa cctcgccgtg ggcgaagttg
      781 atcatgccga taatgccgta aaccatggtg tagccgatgg ctatcagcgc gtaggtactg
      841 cccagcgtga cgccgttaaa catctgctgc aagaaataca aaaactgctc agacataagg
      901 taacctttct aaaaccgccc gcatttcacg ggcggtggga tgatcacttg gctgccgtgg
      961 atgaaccgtc ggcgtgccac tggaagacac caaaatcgaa tcccttaaga tcgccttttt
     1021 catcccagtt cagcggccca attacggtgt ttgcaccgtt agcttttaaa tctttcacca
     1081 gcgccagcgg ctcatcgctg ccggtacgtt caagggcagt tgccagagat tgcaccgccg
     1141 cgtaggtgat ccagacatat ggcccggacg gatctttctt gtcggctttc agcgcatcaa
     1201 cgatgccctg gtttgtcgga tcctggtcat agcgttttgg catggtgacc aacatgcctt
     1261 cagcggcatc accggcaatg ttcgacagtg acgcattacc gacaccttcc ggccccataa
     1321 actgggtttt caggccaacg gaacgggcct ggcgcagcat ctgccccatt tccgggtagt
     1381 aaccgccgta gtaaacgaag tcgatgtttt cttttttcag gcgggcgatc agcgcggaga
     1441 aatctttctc tccagcggta atgccgtcga agaagacgac gttggcgtta gccgctttca
     1501 gcccgtcctg caccgaacgt gccagccctt cgccatactg ttgtttgtcg tgaatgatgg
     1561 cgatgcgctg gggcttcacc gtctcaagaa tgtattttgc cgccgttggc ccctgggaag
     1621 agtccagccc ggcagtacgc atgatgtatt gataaccgcg ttgggtcagc tccggattgg
     1681 tcgctcccgg cgagatcatc agaatacctt cgtcttcata gatatctgat gcaggttggg
     1741 tagaggagga acacagatga ccaataacat atttaatgcc gtcgttaacg attttgttgg
     1801 cgaccgcaac ggcttgtttc gggtcgcagg cgtcgtcata ttccacgcca accagtttat
     1861 cgcccttaat tccccctttg gcattaatgt ctttaattgc ctgacgcgcg ccgttaaatt
     1921 ccatatcgcc ccactgggca atcgggccgg acatcgcgcc gacaacggcg actttaatat
     1981 cgtcagccat agcggtgtgt gaaattgcca gtgcaatcat ccctgcgatg atagttttcg
     2041 cattccgttt catagtcaaa aatccccatt cgtgatgttg tgttgctttg tttttatgtg
     2101 ttaacaaatc agactgttct ttttttatac tgcactgttt ttacctgtct gattaagggt
     2161 ttagcgcagt attttgtgat gatagcgatt aaaatcccta tttttcagtc
//
LOCUS       DAWDRC010000637         2205 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17606_length_2205_cov_2.222326, whole genome shotgun sequence.
ACCESSION   DAWDRC010000637 DAWDRC010000000
VERSION     DAWDRC010000637.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17606_length_2205_cov_2.222326"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1103..1112
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          579 a          547 c          580 g          489 t
ORIGIN      
        1 acggattcat acatgtattc ccagtgaaaa aagacaaaac cccgtgcacc ggaaaggtgg
       61 cgggggattc acggagataa tatctttctg aaattatttt gaatgcaggt atatgtggct
      121 gatttgtatc agctaatgag tacaggtctg aatctaatct gacagtccgc tttgtgccaa
      181 aggcggacgc tcaagtagat actgtcctga cagtatgcgg taatcagagt tgttatatat
      241 tacttgcgtc caaaccagct ttctgacaac ccggccgctc ctgctttcaa caacgcgccg
      301 ccatactttt atgctgcaaa gagcggagac agaaggttag cgtaatttgt aaataactta
      361 cattcacgtg tgatctggat cataattttg cctatgcacc gatcgtaata taaatattta
      421 cataacgatc agtgattcat gaaaactcaa ataactttcg ctgcgctttt gccagcatta
      481 gcgtctttca taccgcttca tgctcatgcc tcgtctactt ctgaagatga aatgattgtc
      541 acgggcaaca ccgccgccga caccaccgat tctgccgccg gtgccggttt caaaacgaac
      601 gatatagatg tcggcccgct gggaacgaaa tcctggatcg aaacaccata ttccagcacc
      661 actgttacta aagagatgat tgaaaatcag caggcgcaaa gcgtcagcga gatgctgaaa
      721 tactctccca gtacgcaaat gcaggcgcgc ggtggaatgg atgtcgggcg tccgcaaagt
      781 cgggggatgc agggcagcgt ggtggccaac agccgtctgg acgggctgaa tatcgtttca
      841 acaaccgcgt ttccggtgga aatgcttgag cgcatggatg tgcttaacag tttgaccggc
      901 gcgctgtacg gcccggcgag cccagcaggg cagtttaatt tcgtggcgaa gcgcccaacc
      961 gaagagacgc tgcgtaaagt gacgctgggc tatcaaagcc gcagtgcgtt taccggccat
     1021 gccgatctgg gtggccattt tgatgaaaac aaacggtttg gctatcgcgt gaacctgctt
     1081 gatcaggaag gggaaggcaa tgnnnnnnnn nngtagcttt aactatgggc cgaacgtcaa
     1141 actgccgtct gcgcagaatc cgaaggacaa aaatctggcg ctcagcactg cgggcaacga
     1201 cctcactacc gataccatca gcactcgcct gatccactac tttaacgacg actggtccat
     1261 gaacgctggc gtgggctggc agcaggctga ccgcgcgatg cgtagtgttt ccagtaaaat
     1321 actcaacaat cagggcgata tctctcgttc gatgaaggat tccaccgctg ccggacgttt
     1381 tcgcgtcctg agcaacaccg ccgggctgaa tggtcatatt gataccggct ctatcggcca
     1441 cgatctgtca ctttctacca cgggatatgt ctggtcgctt tatagtgcca aaggaacagg
     1501 ttccagctat agctggggta caacaaatat gtatcacccg gatgcgatag atgagcaggg
     1561 cgatggcaaa atccgcaccg gcgggccgcg ataccgctcc agcgtaaata ctcagcagag
     1621 cgttacgctc ggcgatacgg tgacatttac gccgcagtgg tcggcaatgt tctatctcag
     1681 ccagagctgg ctgcagacta aaaactacga taagcacggt aatcaaacga accaggttga
     1741 tgaaaatggt ttaagtccga acgccgcgct gatgtataaa attaccccta acacaatggc
     1801 ctacgttagc tatgccgatt cgctggagca gggcggtacc gcaccgacgg atgagagcgt
     1861 aaaaaatgcc ggtcaaacgc taaacccgta tcgcagcaag cagtatgaag tggggctaaa
     1921 atcggacatc ggcgagatga atctaggcgc cgcgctgttc cgactggaac gtccgtttgc
     1981 ctatcttgat acggataacg tgtataaaga gcagggtaac caggttaaca acggccttga
     2041 gttaaccgct gccgggaatg tgtggcaggg gctgaatatt tacagcggcg tgaccttcct
     2101 cgacccgaaa ctgaaagata cggcgaatgc ctcaaccagc aataaacagg ttgtcggcgt
     2161 gccgaaagtg caggccaatc tgttggcgga atacagtttg ccgtc
//
LOCUS       DAWDRC010000638         2181 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17812_length_2181_cov_2.274694, whole genome shotgun sequence.
ACCESSION   DAWDRC010000638 DAWDRC010000000
VERSION     DAWDRC010000638.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17812_length_2181_cov_2.274694"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1371..1380
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1769..1778
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          467 a          615 c          540 g          539 t
ORIGIN      
        1 cgcgcttagc caacgaccag attgaggagt ttacctggta cgtaaatcac tttacgtaca
       61 gtaacgccat caagatattt tgctaccaga tgttcctggc cagcacgttc gcgaacctgt
      121 tcttccgttg cgtccaccgg aacggtgatt tttgcacgga ctttaccgtt aacctgcacc
      181 acgaccagcg tggagtcttc caccatcgct ttttcgtcag caaccggcca cggcgcgttg
      241 tcgatatcgc cttcgccttt cagttcctgc cacagcgtga agcagatgtg cggggtgaac
      301 gggttaagca tacggactac cgccagcagc gcttcctgca tcagggcgcg atcctgctcg
      361 ccatcggtcg gtgctttcgc cagtttgttc atcagctcca taatcgccgc aattgcggtg
      421 ttgaaggtct gacgacggcc gatatcatcg gttactttag cgatagtttt atgcacatcg
      481 cgacgcagcg ctttctgatc ttcagtcagc gcatcgacgt tcagtgccgc aacatcacct
      541 tttgctgtgt gctcgtacac cagtttccag acacgtttca ggaagcggtt agccccttcc
      601 acgccggact cctgccattc gagagtcata tcagccggag aagcaaacat cataaacaga
      661 cgaacggtgt ccgcgccgta acgttcaacc atcacctgcg ggtcgatacc gttgttcttc
      721 gacttggaca ttttgctcat gccggtataa accagttcat ggcctgccgc atctttcgct
      781 ttcacgatac ggcctttctc gtcacgttca acgatagcat caaccgggga aacccagtta
      841 cgttcgccgt tttcaccaac atagtagaag gcatctgcca gcaccatacc ctgacacagc
      901 aactgtttcg ctggttcgtc agagttcacc atgcctgcat cacgcatcag tttgtggaag
      961 aagcggaagt agagcaggtg cataatggcg tgttcgatac caccaatgta aatatccacc
     1021 ggcagccagt agttagccgc tttggaatcc agcatacctt ctttgtactc cgggcaagtg
     1081 tagcgcgcat agtaccagga ggactccata aaggtgtcga aagtgtcggt ttcacgcagt
     1141 gctggcatac cgttaacggt agtttttgcc cactccggat ctgctttaat cgggctggta
     1201 atgccgtcca tcaccacatc ttccggcagg atcaccggca gctggtcgtc cggggtcggc
     1261 attacggtac cgtcttccag cgtcaccatc ggaatcggcg cgccccagta acgctgacga
     1321 gaaacacccc agtcgcgcag gcggtagttc actttacgct cgccaacgcc nnnnnnnnnn
     1381 ccaacgccca tcgcagtcag tttatcggcg atggcgttga aggccgcttc atggtcaaga
     1441 ccattaaact cgccagagtt gaacagcacg cctttttcag tcagggcttg ctgagaaaga
     1501 tctggctcag agccgtcagc tgccaggata accggtttga tgttcaggcc gtatttagag
     1561 gcaaactcgt agtcgcgctg gtcgtgaccc ggaactgcca taactgcgcc cgtgccgtat
     1621 tccatcaata cgaagtttgc tgcccaaacg ggaatttctt cgcccgttaa tgggtgaacc
     1681 gctttaaagc cagtatcgac gccttttttc tccatcgtcg ccatttcagc ttcggcaact
     1741 ttggtgttac ggcattcgtc aataaaggnn nnnnnnnnat aaaggcggcc agttcaggat
     1801 tattttccgc cgctttctgt gccagcggat gacccgcagc caccgccagg taggtacaac
     1861 ccataaaggt gtccgggcgg gtagtgtaaa cggtcagcgt gttgtcatag tcgttaacgt
     1921 tgaaggtgat ttccacgcct tcggaacgac cgatccagtt acgctgcatt gttttaacgg
     1981 tgtctggcca gtgatccagt ttatccagat cgttgagcag ctcgtcagcg taagcagtga
     2041 ttttgataaa ccactgcggg atctctttac gctcaacttt ggtatcgcag cgccagcagc
     2101 agccgtcgat aacttgttcg ttcgccagta cggtctggtc gttcgggcac cagttgaccg
     2161 cagaacgctt cttatatacc a
//
LOCUS       DAWDRC010000639         2176 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17850_length_2176_cov_2.730316, whole genome shotgun sequence.
ACCESSION   DAWDRC010000639 DAWDRC010000000
VERSION     DAWDRC010000639.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17850_length_2176_cov_2.730316"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1662..1671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1946..1955
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          646 a          493 c          419 g          598 t
ORIGIN      
        1 ttcctgaatt tgcgttgaga agcctcggga aatagatgtt tttagttgct cccaacaata
       61 tattaactcg gggttctgag gtaagcagaa acctgtcgtg ttacgaattt gatcagttat
      121 cgcataaaat ttttgaaact tttcaattag atcaatggga taataaagca tttctgcaag
      181 ataaagacct gcttctggat aattcgcaat ataaaattca tatccacagg gaaataatat
      241 tatctgatta ttatcaacag ttagactatg cgtctcccaa ttgataactt tttttccctg
      301 acggatacga cataaagctg gcataagagg cttaaccctg tgaatctcat gatgtttatg
      361 catccgtatt tcttcaatct ttaggttagt cttatctttt gccagcatac tcttacccta
      421 ctttatctca taaactggtg ttatctcagc ggttgcaatt ttattggcat taagcatata
      481 accaactaac gctccgctgg aattagaatc cacaggaatc ttatcagttt ttagagccca
      541 tactttaaac tggtaatgat ggggtttatc tcctttggga ggacatgctc caccaaatcc
      601 tgcataacca aaatcatttc ggccttgaac agcaccagtc ggaagttttg taccatcacg
      661 tcttcctgca tcagcgggca aatatgttac agttgctgga atattagcaa cagtccagtg
      721 ccaccatcca ctgcctgtag gagcatctgg atcatacaca gttacggcaa agcttttggt
      781 gccttcagga gcaccagacc aggttaatga gggcgatgta ttaccacctt cacacccaaa
      841 tccagaaaat acatgagacg ttgtaagttg ctctcctgtt tttatttcat tactagtgac
      901 ctgaaatgct gcggcctgcg cagaaaatgt tatgaatgcc aatacacttg aaacgataag
      961 ttttttcata aaaacctcat tgttgtgacc tatcgttatt ttatttgatg tgccttgatc
     1021 tcattatgca taaaggcgca atgatcgcgc aaatgcaatc ataattataa aggtgccccg
     1081 aacattagcc ataatatgca caaatcagat tcctgttatc tcaaggataa ttatttttta
     1141 atgcggtgca cttcgtgaca acaagttaat gactaacaca ccggcacaaa tcaacatcat
     1201 gcctatgata gctggcaggt ccagtcgttg accaaaaaat ccccaggaaa gcaaactaat
     1261 cagaacgata ccgactcctg accagatagc ataagcaatc cctgtaggta tataagccag
     1321 cgtctgagct aataaccaga atgatgcaca atagcaaata attgtgccaa cagagggcca
     1381 taaccgtgta aacccctctg aaaacttcat taaggttgta ccaatgacct ctgcaagtat
     1441 tgcgccacca agataaatat aagggttcat agcatattct ttcctgttca tactggagag
     1501 aattgtacta cagtttgaac acaactcacc gttttcatcc tggtgtatgt gtatccaggg
     1561 ggctctggcg tggcaagaga taaaggcagg aacaataatg atcagaagga accccaggct
     1621 aacgaatgag ttcactcttc ctgggagcaa agcgaagaca annnnnnnnn nacaaaacat
     1681 gttatcgcgt ttacgcatcg tggatggcag aaaactttca gaatcaggtt tccattttaa
     1741 accagaaatt aagtgagttt gtcccatcca tgccccacgc ggtgagatcg gatgtaatca
     1801 acaacaggtt acaaaaccta cacctgcatg gacatcactt cctgatacgc cgccaccagc
     1861 ttattacgca cctgaatccc catttgcata gaaactgagg ctttttgcat atcggtcatc
     1921 acatcgttta acgccacgcc gggttnnnnn nnnnntgtat cgcttatgcg atcgagcgcg
     1981 gcgtgcagct gcccggcaaa actaatggtc ggttgcggca gtgattcctg cgcacgcgca
     2041 ctcatcgccg tagcctgtaa ctggctgata accccttcaa tcccctgtat cgctgacatt
     2101 ttcgtctccc ggataatttc tggtaacaaa gcctaccagt aagtcaataa gacaaaggcg
     2161 ctaaatagca acaaaa
//
LOCUS       DAWDRC010000640         2172 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17877_length_2172_cov_2.042513, whole genome shotgun sequence.
ACCESSION   DAWDRC010000640 DAWDRC010000000
VERSION     DAWDRC010000640.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17877_length_2172_cov_2.042513"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    873..882
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          536 a          540 c          587 g          499 t
ORIGIN      
        1 gggtgttgaa taattcccct gatattgcaa gggctattca ggccattgat atcgagaaac
       61 tctaagaaac catagtgatg ttttttgaca atgtgttagc tctttatcaa taggccgcac
      121 ggttgtttgg tggcgtcaaa atcgccgagg cgttccctga aggccggggc agcccacatg
      181 gatgtgggct gagggcgcgt tttacaggga tgttacctcg cgcccgaccc ggtagccgta
      241 agggataagc cgagggcacc gcgcagcggc gattttgttc gccagagccc gggggtgcag
      301 ggggcggcgg cgactggtcg ccccctgcgc gctccttgcg ccagtgatta tatgaagcaa
      361 tgcacatgac aggagcgcaa caggatgaga gactgaaata ttctcgttat tttcggatct
      421 gatgacgtta attaatttta aaatatcatc ttaaatacgc cagtcaccac cagtagccca
      481 atcaggaaaa taatcaatac agcccagagt ataattttca ttggcgttcc tttatttgtg
      541 agtgctgaat aagtatagga aaaaacgaag ccctttgccg ttatccataa acgattaaga
      601 taaatcgaaa gatatcattc acttagtgta tatttttctg gatatgtcgt tttattgcta
      661 ctgatgtatt gccacgcgaa actaaaaaat atatcgcaac acacggtaat ctccgcgcac
      721 cttttcttac ctgattattc ccccatgaat cgtgattcct tttatcccgc catcgcctgt
      781 tttccgctgt tactgatgct ggccgggtgt gcacctatgc atgaaacccg ccaggtgtta
      841 agccagcaaa cgcccgctgc acaagttgac acnnnnnnnn nnaccgtatt acccacggcg
      901 ctgaaaaatg gttggccaga cagccaatgg tggctggagt atcacgataa tcaactcact
      961 tccttaatta ataatgcact acagagcgca ccggatatgc aggtggctga acagcgcatt
     1021 cagctggctg aagcccaggc gaaggcaatt gccactcagg atggtccgca gctcgacttt
     1081 tctgcggata tggagcggca aaaaatgtcg gcagaagggt taatggggcc gtttgctctg
     1141 aacgatcctg ccgcaggtac gaccggtccg tggtacacca acggtacttt tggcttaacg
     1201 gcgggctggc atctcgatat ctggggaaag aatcgggcgg aggttactgc tcgcctcggt
     1261 acggttaaag cacgggcggc ggaacgcgag caaacccgcc aattgctggc tggcagcgta
     1321 gcccgcctgt actgggagtg gcaaacccag gcggcgttaa acacggtctt acagcaaatc
     1381 gaaaaagagc agaacaccat tatcgccacc gatcgccagc tatatcagaa cgggattact
     1441 tctgcagttg aaggtgtgga aaccgatatt aatgccagca aaacccggca gcagctcaac
     1501 gatgtcgcgg ggaaaatgaa aattattgag gcacggttaa gtgcgctcac caaccaccag
     1561 acaaagtcat tgaagcttaa accggtcgcg ttgccgaaag tggcaagtca gcttcctgat
     1621 gaactcgggt actccttact ggcccggcgg gcagatttgc aggcggcgca ctggtacgtt
     1681 gagtcatcgt taagcactat tgatgcggca aaagcggcat tttatcctga catcaacctg
     1741 atggcctttc tgcaacagga tgcgttgcac ttaagcgatc tgttccgtca ttccgcgcag
     1801 caaatgggcg ttacggcagg cctgacgcta cccattttcg atagtggtcg tcttaacgcc
     1861 aacctcgata tcgcaaaagc cgaaagcaac ttgtctatcg ccagctacaa caaagcggtg
     1921 gttgaagcgg tgaatgacgt ggcgcgggca gccagtcatc aggcgcaaat tgagcgcgat
     1981 gccttgcgtg tagtaggtct tgcgcaggcc cgctttaacg cgggcctcat tgctggttcc
     2041 cgggtcagcg aagccagaat cccggcgctg cgtgagcggg ccaatggcct gttattgcaa
     2101 gggcagtggc tggatgcctc cattcaactc actggtgcgc tgggcggggg ggacaaacgc
     2161 tgatgatatg cg
//
LOCUS       DAWDRC010000641         2170 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17893_length_2170_cov_1.897872, whole genome shotgun sequence.
ACCESSION   DAWDRC010000641 DAWDRC010000000
VERSION     DAWDRC010000641.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17893_length_2170_cov_1.897872"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          496 a          587 c          577 g          510 t
ORIGIN      
        1 ggtgccgatg ggatggctgg caaagttact gagggtttcc cattcgcgcg gcaggatcat
       61 cgccggaact tgccccagca gtaacggtaa ccagcggtcc ggttgcgtga gatggatatc
      121 cagcgtccag ggcgtcggcg acacaatgtc agcaatatgc gaatagagcg gcagcgtatt
      181 gattcgtttt aaagaggcga tcacgtcgtc catttccagt tcacgaccat gatgaaaatg
      241 gactcctgga cgcaaaaaga aacgccagtg aagcggtgaa atttgctgcc agtggtgggc
      301 gatgtccgct tccagttccc cattttcctc atttatgcgc gttagcgaac tgaagatttg
      361 ccgggcgata tgggtttcgg aacggcgcaa tgcactgccg ggcagcagat tacgcaacgg
      421 acgatagtag agcacacgca ggatgtgccg cccctggcgg aagctgcgtc ccagatgaga
      481 aaccaacatt tgccgcacag ccgctttgtc gccaaccagt tgcaccagtt gatcgatacg
      541 atcctgctcc agcaggtcct ctgctcgctg ttgctgaagc gccagcccgg tatagaggaa
      601 tgtcagacgc gagcgtttgc cgcgcccgac ttccgcttcc cacgtcagcc agccgcgatc
      661 ctgcatggtg ttgagcaggg tacgcatatg acgacgcgag cagctcaata acgctgccag
      721 ttcgttgagc gttgtgtcct gcgatttacc ctcgcagcat tgccacaggc ggatgaactg
      781 ttgttgcaga cgagcagatg gcataaaagg ggaactcctg cgcaaaagac agcaatttta
      841 ttttccctat attaagtcaa taattcctaa cgatgaagca aggaggtgcc ccatgcgtca
      901 gttttatcag cactatttta ccgcggcagc gaagttgtgc tggttgcgtt ggttaagcgt
      961 cccacaacga ttaaccatgc ttgaagaact gatgcaatgg gaggggagtc attctgacta
     1021 ctgatttgcg gacatcatgt gtgactgagt attggtgtta atcgccacgc cagcagtgat
     1081 tatctgctgg cttttttctt tttggcgatt gagtaccgga cggtttgcaa actaacgcat
     1141 aaattccggt tgcttctctt cataagacgc aatagcgtcg tcgtgctgca gggtcaggcc
     1201 aatgctgtcc agaccattca tcatgcagtg gcggcggaag gcatcgatgg taaagcgata
     1261 agttttctct cccgctttca cctcttgcgc ttccaggtct acatcaaaat gaatccccgg
     1321 attggctttc accagcgcaa acagctcatc cacttctgca tcgcttaatt tcaccggcag
     1381 cagctggttg ttaaagctat tgccgtagaa gatgtcagca aaactcggcg caatcaccac
     1441 cttaaaaccg taatcggtca atgcccacgg cgcgtgctca cgcgaggaac cgcagccgaa
     1501 gttttctcgt gccagcaaaa tggaagcgcc ctgatactgc gggaagttca gcacgaagcc
     1561 cgggtttggc tgttggcctt tttcatccag aaaacgccag tcgttaaaca gatgcgcgcc
     1621 aaaacccgta cgggtcactt tctgcaaaaa ctgtttcggg atgattgcat cggtatcgac
     1681 attggcggca tccaacggaa ccaccaggcc tgtgtgtttg ataaatttct ctgccatggt
     1741 gtgctcctta tttaatgttg cgaatgtcgg cgaaatgtcc ggtcacagca gcagcggcag
     1801 ccattgccgg gctgaccaga tgggtgcgcc ctccacgccc ctggcggcct tcaaagttac
     1861 ggttgctggt ggaggcacaa cgctcacccg gattcaggcg atcgttgttc atcgccagac
     1921 acattgagca acctggcaag cgccattcaa aaccggcttc gataaaaatt ttgtccagac
     1981 cttccgcttc cgcctgggct tttaccgggc cagagccggg aaccaccagt gcctgcacgc
     2041 ctggcgcgac tttccgtcct ttggcgattt cagccgccgc gcgtaaatct tcaatacgcg
     2101 agttagtgca ggaaccgata aacactttgt cgatagccac ttctgtcagc ggaatacccg
     2161 gtttcagccc
//
LOCUS       DAWDRC010000642         2169 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17899_length_2169_cov_2.070956, whole genome shotgun sequence.
ACCESSION   DAWDRC010000642 DAWDRC010000000
VERSION     DAWDRC010000642.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17899_length_2169_cov_2.070956"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    491..500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1759..1768
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          494 a          607 c          566 g          482 t
ORIGIN      
        1 agctgcacgg tcgttagccg ccagggcaaa gaacatgccg taactttgcc cttcggaggt
       61 ggtgattttg cgcgcgtcgc tggggtcgat gacgcgccct tcctgactga tgtaatcctt
      121 tttaaactgc tcccaggcag gccaggtaca ggctgcctga acactaaagg cagccagcag
      181 cagcatcgtc acgagtccac tacgcaacac attcatcttc aattactcgt tatccgggtt
      241 aagacgacga cgactaataa tacgcagcag acgccacagt acccacgcca gcaatatcac
      301 actgatagcc gccagcaccg ccagcagaat cggatggttt gccagcgcat accacaagcg
      361 ctcgaaccac ggcagatgac ctacgtaata aacatcgcca acacgtaggc tgttgatacc
      421 ggactcgcgg atcaccgcga ccgaaccgaa catggtggcg cgtttgccgc tatcgttcac
      481 cgcatcgtta nnnnnnnnnn gcatcgttaa gcatttcata accgcgtggg ctatctgcca
      541 gcagcgcaat cacgctgcgc tggtcgttat acggcgactg gaaaccaatc accgccgcca
      601 tcgcaccgga agagatcagc gttgactggg tttctgccgc gcgatcgctc tcgtccggca
      661 caataccggg gaacggggtc tggcgcatcg gcgttttcac ccagctttcg gtagcctgca
      721 ccaacaggtc gatctgctta tcgtctttca gtttgtccgg gataccaccg acgatcatga
      781 tgtcggcatc tttgccctga atggtgctgc catcatcggt caccgtcagg ttaatcgccg
      841 ggaagcccgt ctgcgcaccg ataaagccaa cggtattcag caaggtttcc atctgtgctt
      901 cgtttggcgt tttcggcatc acggtgatgg tttgcgacaa atccgccatc cggctgaacg
      961 ggaagcccgc gttagcaaag gcgcgtagat ccggcatcgg gatgaagtgg taatacttcg
     1021 agaagtcgat ggtggagtcg tcaccaatca ccacatgatt ctgcaccggc tggaaggtaa
     1081 tgcagttatc caccgaaccg cccggcatcg ggttcatata ctcaaagtcg aaccgcagct
     1141 ggttggtcgc gcccagtttc agcgccggaa tagagacatc cgttttgcca tccagcaaac
     1201 cttgtaatac cggaatccgc agcagcaggc ggttcgcctc ctgtttgctg ctcaggttga
     1261 aggattgcag gaactggtta ttcaggctga tatccatccg cgaactgtct ttcaccggcg
     1321 gcatggtgta gcggtaatta atatccatat caatgccggt actgcgcatc aggtagagat
     1381 ccggcggcag gtttagcgaa acgttaatcg ctgctggctc aagaccgctg gattgtaatt
     1441 gttcttcata ggttttcagt tcaccaaagg tgaccggacg atcggtacgc acccagttcg
     1501 gcgcatcgta cggcttacgt ggtagcagcg gtttcacttc attcactacc acactttcac
     1561 cacggaacag aatgttaccc tgagcgatac ctttcgctgc ctgcaacaag tctttgtcgt
     1621 cacgaccaaa caccaccagc agtttgacgt aaggattctg cggatggtta atcatctcaa
     1681 tcaccggggc ttttaccgcc ggatgatcgc gcaggaagtc cgggcgtttg tcattggtgg
     1741 caaagacaat ggcattgcnn nnnnnnnntt gcgatccggc agttggttat agagcaccgg
     1801 gaagttctgc ccacgccagc cagaacgcga accaaaccac gaggcgacaa tggcagatgc
     1861 ttgttgcagc tcaacatccg gcgcacccgc aaagaccatc ggcaaggtgt tggtgcggtt
     1921 atcacgcggg tcaaagaatg gcaccgggaa gtgtgacagg tcattcttca cattcagggt
     1981 ctgataggtc agatccagtc cactgctccg cccaacatcc agccaaagcg tggtgctggc
     2041 cgggttttcg cacacgtcct gataatggcc gacaaactcc agccgcacac ggttgaaatc
     2101 agtaataaac agcgagttaa tgggcatttg tgccagcgtt tttttaccca actgttcttt
     2161 ggtcactgg
//
LOCUS       DAWDRC010000643         2168 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17916_length_2168_cov_1.724562, whole genome shotgun sequence.
ACCESSION   DAWDRC010000643 DAWDRC010000000
VERSION     DAWDRC010000643.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17916_length_2168_cov_1.724562"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    454..463
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1896..1905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          497 a          568 c          580 g          503 t
ORIGIN      
        1 tgaccgacat agtgtgccca gccgccaccg ctttgcccga cacaaccgca gaagatcagc
       61 atattgatca tcccgcggta gttcatgtcc atgtgatacc agtggttaac acctgcgccg
      121 agaataatca tcgagcgccc atgcgtttta tgggcggtat cggcaaattc acgggcgatg
      181 gtttcaatat actggcgcgg aacgccggta atttgctcgc cccaggcagg ggtataaggt
      241 ttgatttcag cgtaatcttt cgcgctgttt tcatcttcca gcccgcgatc gaggccgtaa
      301 ttcgccagta ccaaatcata aacgctgacc accggacaga cagtgccatc ggcgagcgtc
      361 agatttttca ccggaagctg gcgcaccagg acaggattgt gttttacgct gcgaaaatgt
      421 ggattttcaa tgccgccaaa gtaggggaag gccnnnnnnn nnngaccgag cagggttaac
      481 gacaattcgg tttccgtacc ggcggcaatg gattccaggt tccatttgcc tttttctccc
      541 cagcgaaaac caatcgaacc gttcggcact accaattcac ctgcggtatt aactgccacg
      601 gttttccact gcggattatt gctttcgccc agtccatcca ccaggtcaga tgcgcggacc
      661 atgcgcccgg gaacgtagct gccgtcgtcg cgaggttcca gcattaccag catcggcatg
      721 tcgctgtagc ggcggcagta gttgataaaa taatcgctgg gattatcgag atgaaactct
      781 tttaaaatga catggcccat tgccattgcc agggcactat cagtgccttg tttcggtgcc
      841 agccactggt cgcacaattt ggccacttca gagtagtcag gggtaatggc gatggtttta
      901 gtgcctttgt agcgtacttc ggtaaagaaa tgggcgtccg gcgtacgtgt ctgcggtacg
      961 ttagaccccc aggcgatgat gtaactggag ttataccagt cggcagattc cggtacgtcg
     1021 gtttgctcgc cccaggtcat cggcgaggcg ggcggcaggt cgcaatacca gtcgtagaag
     1081 cttaaacagg tgccacctaa cagcgacaga taacgtgttc cggcggcgta agaaaccatc
     1141 gacatcgccg ggatcggcga gaaaccggca acgcgatccg ggccgtaagt tttgatggtc
     1201 cagacgttag cagcggcaat cagctggttt agctcctgcc agttggagcg gataaagccg
     1261 ccgcgtccac gtacttgttt gtagctcagg cacttttgcg gatcgttcat aatcgatgcc
     1321 cacgccagta ccggatcgct gtgttgcttg agggcttcgc gccacagttc aatcagtcgt
     1381 ttacgaatga gcgggtattt caggcggtta gcgctgtaga gataccagga gtaacttgcg
     1441 ccacgcgggc agccgcgagg ttcgtgattt ggcaggtcag ggcgagtgcg cgggtagtcg
     1501 gtctgttgga tttcccaggt caccagacca tttttaacgt agattttcca gctacaggag
     1561 cctgtacagt taacaccgtg ggtggaacgc acgattttgt cgaactgcca acgctggcga
     1621 tagctgtcct cccagtcgcg gttgctatgc atcacctgtc cgtgaccatc ggcaaaggtt
     1681 tcgccctttt gtttgaagta gcgaaagcga tccaaaagtt tactcatgac ataactcctg
     1741 ctccaggaat gaattgggat ctgatgcctt gtcggatgag acacggttgt agcctgataa
     1801 gacgtgtcag gcatcgatct cttatttttg gctgaacttc cgccgaccat aaatcagcca
     1861 ggtcagtagc acacaaacga tgtagaagat taaaannnnn nnnnngatta aaaacacttt
     1921 catcgcgccc accggagagc cggtcatatt gagcgacatg ccaaacgcct gcggaataaa
     1981 aaagccgccc actgcgccaa tggctgagat aaagcccaga gcggctgccg tttcggtgac
     2041 ggcttctctt tgagcttgct catcactacc gcctttcatc ttcacccgat aaatggttat
     2101 ctggcgaaag atgacggcga tcatctggaa agtagaacca cttcccagac ccgcggtcag
     2161 aaacagcc
//
LOCUS       DAWDRC010000644         2167 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17924_length_2167_cov_2.223958, whole genome shotgun sequence.
ACCESSION   DAWDRC010000644 DAWDRC010000000
VERSION     DAWDRC010000644.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17924_length_2167_cov_2.223958"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    364..373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          522 a          536 c          649 g          450 t
ORIGIN      
        1 cgttgaggcg tctattcagg tcaaaccgtc ttatggcctg accgatagcg aaatcgcctc
       61 gatgatcaaa gactcaatga gctatgccga gcaggacgta aaagcccgaa tgctggcaga
      121 acaaaaagta gaagcggcgc gtgtgctgga aagtctgcac ggtgcgctgg ctgctgatgc
      181 cgcgctgtta agcgccgcag aacgtcaggt tatcgacgat gctgccgctc acctgagtga
      241 agtggcgcag ggcgatgatg ttgacgccat cgaacaagcg attaaaaacg tagacaaaca
      301 aacccaggat ttcgccgctc gccgcatgga ccagtcggtt cgtcgtgcgc tgaaaggcca
      361 ttcnnnnnnn nnnaatatgc caaagattgt tattttgcct catcaggatc tctgccctga
      421 tggcgctgtt ctggaagcga atagcggtga aaccattctc gacgttgcgc tgcgtaacgg
      481 tatcgagatt gaacacgcct gtgaaaaatc ctgtgcttgc accacctgcc actgcatcgt
      541 tcgtgaaggt tttgactcgc tgccggaaag ctcagagcag gaagacgaca tgctggacaa
      601 agcctggggg ctggagccgg aaagccgttt aagctgccag gcgcgcgtca ccgacgaaga
      661 tttagtggtt gaaatcccgc gttacactat caaccatgcg cgtgagcatt aacagaggtt
      721 agtatgggac ttaagtggac cgatagccgc gaaattggcg aagcactgta cgatgcgtat
      781 cccgatattg atccgaaaac ggttcgattc accgatatgc atcagtggat ttgcgctctg
      841 gaagatttcg acgacgacac gcaggcatcg aacgagaaaa tcctcgaagc gattttgttg
      901 gtctggctgg acgaagccga gtgaatgaac gggctgcctg ggggtagccc gttttgctgt
      961 taaacgaata aggataacta aaatgacaga agcgatgaag attaccctct ctccccaacc
     1021 tgccgacgca cgttggggag aaaaagcgac ttacagcatt aataatgatg gcattaccct
     1081 gcatttgaac ggggcagacg atctggggtt gatccagcgt gcggcgcgca agattgacgg
     1141 tctgggcatc aagcatgttc agttaagcgg tgaaggctgg gatgcggatc gctgctgggc
     1201 attctggcaa ggttacaaag ccccgaaagg catacgtaaa gtggagtggc cggatctgga
     1261 cgatgcccag cgtcaggaac tggataaccg cctgatgatc atcgactggg tgcgtgacac
     1321 catcaacgca ccggcagaag agctggggcc atcgcaactg gcacagcgtg ctgttgatct
     1381 gatcagcaac gtcgcagggg atcgtgtgac ttaccggatc accaaaggcg aagatctgcg
     1441 tgagcaaggt tatatggggc tgcacacggt cgggcgcggt tcagaacgtt ctccggtatt
     1501 gctggcgctg gattacaacc caactggcga taaagaagcg ccagtgtacg cgtgcctggt
     1561 tggtaaaggc atcacttttg attccggcgg ctacagcatc aaacagactg cgtttatgga
     1621 ctcgatgaag tcggacatgg gcggcgcggc aacggttacc ggggcgctgg catttgccat
     1681 tacgcgcgga ctgaacaagc gcgtgaagct gttcctctgt tgtgcggata acctgatcag
     1741 cggcaacgcg ttcaaactgg gcgatattat tacctatcgc aacggtaaaa aagttgaagt
     1801 gatgaacacc gatgccgaag ggcgtctggt gctggccgat ggcctgattg atgccagtgc
     1861 gcagaaaccg gaactgatca ttgatgcggc gaccctcacc ggggcggcga aaaccgcgct
     1921 gggtaatgat tatcacgcgc tgttcagttt tgacgatgcg cttgccggtc gcttgttggc
     1981 gagtgccgcg caggagaatg aaccgttctg gcgtctgccg ctggcggaat tccaccgcag
     2041 ccagctgccg tctaactttg ccgaactgaa caacaccgga agcgcggcgt atccggcagg
     2101 cgcgagcaca gcggcgggct tcctgtcgca ctttgttgag aactatcagc aaggctggct
     2161 gcatatc
//
LOCUS       DAWDRC010000645         2165 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17945_length_2165_cov_2.307109, whole genome shotgun sequence.
ACCESSION   DAWDRC010000645 DAWDRC010000000
VERSION     DAWDRC010000645.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17945_length_2165_cov_2.307109"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1889..1898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          406 a          568 c          649 g          532 t
ORIGIN      
        1 cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc ttccgtttct aatttctctt
       61 ttggggcacg gatttccgtg cccgtttcac aagttggctg ttatgcagga atacacgaat
      121 cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg gcgcacgctt
      181 ttgcatccgt tgtcgttaac ctttcctgcc gggaaagtga ctggtctgat tggtcacaac
      241 ggttctggta aatccactct gctcaaaatg cttggtcgtc accagccacc gtcggaaggg
      301 gagattcttc ttgatgccca accgctggaa agctggagca gcaaagcgtt tgcccgcaaa
      361 gtggcttatt taccgcagca gcttcctccg gcagaaggga tgaccgtgcg tgaactggtg
      421 gcgattggtc gttacccgtg gcatggcgca ctagggcgct ttggtgcggc agatcgcgaa
      481 aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg gctggtcgat
      541 agcctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc gcaggatagc
      601 cgctgcctgt tgctggacga accaacctcg gcgctggata tcgcccacca ggttgatgta
      661 ctggcgctgg tgcatcgttt aagtcaggag cgtggcctga cggtcattgc cgtgctgcac
      721 gatatcaata tggcggcgcg ctactgtgat tatctggtcg ccctgcgcgg cggtgaaatg
      781 attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat gatttatggc
      841 atcccgatgg gtattctgcc gcatccggcg ggtgctgcac ctgtgagttt tgtttattga
      901 tgagcggctt acctcttatt tcgcgccgcc gactgttaac ggcgatggcg ctttctccgt
      961 tgttatggca gatgaatacc gcccacgcgg cagttattga tcccaatcgt attgtggcac
     1021 tggagtggtt gccggtggag ttactgctgg cgctcggcat cgtgccttac ggcgtggcgg
     1081 acaccatcaa ctatcgcctg tgggtcagcg aaccgccatt gccggactca gtgattgacg
     1141 ttggtttgcg tacagaaccc aaccttgaac tgctgaccga aatgaaaccg tcgtttatgg
     1201 tctggtctgc aggatatggc ccttcaccag aaatgctggc acgtattgcg ccggggcgcg
     1261 gatttaactt cagcgacggc aaacagccac tggcgatggc gcgtaaatca ctgacggaaa
     1321 tggcagattt acttaacctg caaagcgcag cggaaacgca tttaacgcac tatgaagact
     1381 ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta ttgctgacga
     1441 cgcttatcga tccgcgccat atgttggtct tcggtccaaa cagcttgttc caggaaattc
     1501 tggatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg ggcagtactg
     1561 ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt tttgatcacg
     1621 acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc atgccgtttg
     1681 ttcgcgccgg acgctttcag cgcgttccgg cggtctggtt ctatggtgcg acgctttctg
     1741 caatgcactt tgtgcgtatt ctggataacg ccatcggagg taaagcgtga gtaaacgaat
     1801 tgcgcttttt ccggtgttat tgctggcgct gctggtggtt gctgctgcgg cgttgacctg
     1861 gatgaacttc tcgcaggcgc tgccgcgcnn nnnnnnnngc aggccacctg gtcgccggat
     1921 attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct ggcgatttcg
     1981 ctgcaggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt gctgcgtaac
     2041 ccgctggcgg aaccgacgac gttgggtgtt gcaacaggcg cgcaactggg gattaccgtc
     2101 accacgctct gggcgatccc cggcgcgatg gcgagccagt ttgctgcgct ggcaggggct
     2161 tgtgt
//
LOCUS       DAWDRC010000646         2159 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_17995_length_2159_cov_2.736217, whole genome shotgun sequence.
ACCESSION   DAWDRC010000646 DAWDRC010000000
VERSION     DAWDRC010000646.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17995_length_2159_cov_2.736217"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          554 a          497 c          583 g          525 t
ORIGIN      
        1 gttgccttct gagtggtagt gttgaagcag tgtagcaggt cacttgctgt acagaatgtg
       61 aattttttaa ccctttgcgc cagccgctgc gatgaggtgt atgattagtc tgacctctgt
      121 ccggttaatg tcgctgagca ggtaatcaga gcacaacaat tacgccaact gcttaggttg
      181 tattgttaaa ggtaaagtga tgagcaaagg aacgaccagc caggatgccc cgttcgggac
      241 attattgggc tacgccccag gtggggtagc aatctactct tcagattaca gttctctcga
      301 tccgcaggaa tacgaagatg acgccgtatt ccgtagctat atcgacgatg aatatatggg
      361 ccacaaatgg caatgcgttg aatttgctcg ccgttttctc tttctgaact acggtgtagt
      421 cttcactgac gtgggtatgg catgggagat tttctcactg cgcttcctgc gtgaagtggt
      481 caatgacaac atcctgccat tgcaggcatt tcctaacggt tcgccgcgtg caccggtagc
      541 gggggcgctt cttatctggg ataaaggcgg tgaatttaaa gacactggcc atgtcgccat
      601 cattacccaa ttgcatggca acaaagtccg tattgcggaa cagaacgtga ttcatacccc
      661 gttgccgcaa gggcaacagt ggacacgcga gctggagatg gtggtcgaaa acggcggcta
      721 taccctgaaa gacaccttcg atgacaccac tattctgggc tggatgatcc agacggaaga
      781 taccgaatac agcttgccac agccggaaat tgcaggcgaa ctgctgaaaa tcagcggggc
      841 gcgtctggaa aacaaaggcc agtttgacgg taaatggctg gatgaaaaag atccgctgca
      901 aaacgcctat gtacaggcca acggccaggt gatcaatcag gatccgtatc attactacac
      961 cattaccgag agtgccgagc aggagctgat taaagccacc aacgagctgc acctgatgta
     1021 tcttcacgcc accgacaagg tgctgaaaga tgacaacctg ctggcgctgt ttgacattcc
     1081 gaaaatcctc tggccacgtt tgcgtctctc ctggcagcgt cgccgtcatc atatgatcac
     1141 tggtcgtatg gatttctgca tggatgaacg tggcctgaaa gtctacgaat acaacgccga
     1201 ttctgcctcc tgtcataccg aagcgggctt gatcctcgaa cgttgggcgg agcagggcta
     1261 taaaggcaac ggcttcaatc ctgcggaagg actgattaac gaactggctg gcgcgtggaa
     1321 acacagtcgt gcacgtccgt ttgtccatat catgcaggac aaagatatcg aggaaaacta
     1381 tcacgcgcag tttatggagc aggcgctgca ccaggcgggc tttgaaacgc gtatcttgcg
     1441 tgggttggat gaactgggct gggatgctgc cgggcaactg attgatgggg aagggcgact
     1501 ggttaactgc gtatggaaaa cctgggcgtg ggaaacggca tttgatcaaa tccgcgaagt
     1561 gagcgaccgt gaatttgccg cagtgccgat ccgtaccggg catccacaaa atgaagttcg
     1621 tctgatagac gttctgttac gcccggaagt gttggttttt gagccgctat ggacggtgat
     1681 ccccggtaac aaagcgattc tgccgatcct ctggtctctg ttcccgcacc atcgttatct
     1741 gttggatact gacttcaccg tgaatgatga gctggtgaaa accggttatg ccgttaaacc
     1801 gatcgccggt cgctgtggca gtaacatcga cctcgtcagc catcatgaag aggtgctgga
     1861 caaaaccagc ggtaaatttg ccgagcagaa aaacatctat cagcaactgt ggtgtttgcc
     1921 gaaagtggac ggtaaataca ttcaggtatg tactttcacc gttggcggca actacggtgg
     1981 gacgtgtttg cgcggtgatg aatcactggt catcaaaaaa gagagtgata ttgaaccgtt
     2041 aattgtggtg aaagagtaat acatgttgta gcaaccccgt actgattatg aagaataatc
     2101 agtacgggga ttaatcaaaa tattcaatat ttatatattt caatatttat tcatttcat
//
LOCUS       DAWDRC010000647         2142 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18163_length_2142_cov_1.760422, whole genome shotgun sequence.
ACCESSION   DAWDRC010000647 DAWDRC010000000
VERSION     DAWDRC010000647.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18163_length_2142_cov_1.760422"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1543..1552
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          487 a          601 c          508 g          536 t
ORIGIN      
        1 taaaatcgct aacgtgccaa tgcttaaggt cacctgacgc gagagcggcc agcgccattt
       61 cagccgatgc gaatgcagtt tttcattaat gagcacactc atgagaacgc ctcccgttgc
      121 tcaaatacgc ggcttaaaac atattcgcgc atggcgatga attgtggatc ggacttgatg
      181 ctgcggctcg actctcccgc aacaaagcgg cgggcgaagt tgagcggcag ccgctccagc
      241 acgcggcctg ggccgggtga aagcagaacc agttcagtcg ccataaacac cgcttcttct
      301 atatcgtggg taatcaacag cacctgcttt cccgtctcct gccagagttt cagcagcagg
      361 gtttgtatct ggtcgcgggt gaaggcgtcc agcgcaccaa acggttcgtc gagtaataac
      421 agctggggat ttgccgccaa cgcgcgggca atccccaccc gctgacgttg accaccggaa
      481 agctgccaga tgtagcgttt ttctgcgcct tccagcccga cttttttcac catctggtgt
      541 gcgatttcca gtcgctgcat tttctctaca cctgccagtt gcaggccgaa cgccacgttg
      601 tcctgtacat tgcgccacgg taacaaccct tcattctgaa aaactacgcc acgctctgct
      661 cccggtccct gaataccctt acccgccagt tgaatgctgc catgctgata aggcacaaaa
      721 ccagcaatca aattcagcaa cgtagttttg ccgcagccag acggccccag aactaccaga
      781 agctcgccgc tttccagcgt caggttgata tcttccagtg ccggtttgcc gccataatcg
      841 gcgtaaagat gagaaatttg cagcatctgc gcctcctttt attgcacgaa gcgcgaggta
      901 acgtactggc tgtaatcatt cgctacagcc gggactttgc cctgctcttt caaaaactgc
      961 gcggtgtcga tgatcgcttt gttcaccggt ccggtcagtt ctgccgtttg ttgctgcggc
     1021 gtcagatagg tattcccctt caccagcccc ggaacgtcac cttccggcac gccgcttaac
     1081 cgcgccagtt tgctgatgtt ttccggctgt ttcagccacg cgtctgggtt agcaatgtac
     1141 ggttgctgag catcgatggc gcttttagcg aacgctttca cgacctcagg atgtttctcg
     1201 gcaaaatctt tccgcacgac ccatacgtcc agtgtcggcg cgccccactg cccgacctgt
     1261 tcagaatcgg tcagcacctt gccatctttt tccagggcgt taaccgccgg tgcccagaca
     1321 taagcaccat caatatctcc ccgctgccat gcggcgataa ttgcgggcgg ctgcaggttc
     1381 acaatctcaa cttgcccagg tttaataccc cagtgtttca gcgccgccag caggctgtag
     1441 tgggtggtgg agataaacgg tacggcgatg cgtttaccaa tcaagtcttc cggtttactg
     1501 atagttttct ttaccaccag cgcttcggag ttacccagtt ttnnnnnnnn nngttttgac
     1561 gccagcaaga agacttcaat cggcacctgt tggctggttg caaccgctaa cgggctggaa
     1621 ccgaggttgc cgatttgcac atcgcccgaa gccagcgccc gcacgatgct ggctccactg
     1681 tcaaacttac gccagtccac ggttgctccg ctttctttag caaaggtgtt gtcggcctga
     1741 gccactttcg ccggttcggc ggaggtttga tacgccacgg tgacgttcac cgcttgcgcc
     1801 tgaaaagcga tgaatgccag tgcggcaaga aatgtgttac gcgatgaaat tgccatgatt
     1861 gtctgctccc ccgtcttgtt atgggagcag tattcaggaa taaaaacatt cattaaaaga
     1921 attagtcgtt atcgtacaga tgattttatt cttagcaaaa aagcggcaaa ggaaagatta
     1981 aatatgcaat tacgcccact ctgataactc agtaaattac tgcacttcat tttattaatg
     2041 cataaacaag caggttctaa tgtctgttat tttattaaca gcaacacata taatgaaacc
     2101 attatattca cattgcatat ataaatgtat cattctcaat ca
//
LOCUS       DAWDRC010000648         2140 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18181_length_2140_cov_1.814868, whole genome shotgun sequence.
ACCESSION   DAWDRC010000648 DAWDRC010000000
VERSION     DAWDRC010000648.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18181_length_2140_cov_1.814868"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    629..638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          496 a          556 c          558 g          520 t
ORIGIN      
        1 tccccttctg acggcctgat gggcggcgga ttcctctata ccaacaaaga ttccgtatcg
       61 ttggggctgg tttgtggatt gagtgatatc gcccatgcgc aaaaaagcgt gccgcaaatg
      121 ctggaagatt ttaaacaaca ccccgccatt cgcccgctga ttagcggcgg caaactgctt
      181 gaatattccg cgcatatggt gccggagggc ggtctggcaa tggtgccgca gctggttaac
      241 gatggcgtga tgatcgttgg tgacgccgca ggcttctgcc tgaatttggg ttttacagtt
      301 cgcggcatgg atttagccat tgcatcagct caggctgccg ccacaacggt gatcgccgcc
      361 aaagaacgcg cggatttctc cgccagcagt ctggcgcaat acaaacgtga gctggaacaa
      421 agttgtgtta tgcgcgatat gcagcatttc cgcaagatcc cggcgctgat ggaaaacccg
      481 cgcctgttta gccaataccc acgaatggtc gccgacatca tgaacgatat gttcaccatt
      541 gacggcaaac caaaccagcc ggtacgcaaa atgatcatgg gacacgcgaa gaaaattggg
      601 ctgatcaact tgctgaaaga tggcattann nnnnnnnncg cgctatgagc cagaacgcta
      661 cggttaacgt cgacatcaaa ttaggcgtca ataaattcca tgttgatgag ggccacccgc
      721 atatcatttt ggcggcaaat cccgatatca atgaattccg taaattaatg aaagcttgcc
      781 ctgccggact ttataagcag gatgacgcag gaaacattca tttcgattcc gccggttgtc
      841 tggagtgcgg cacctgtcgg gtgctgtgcg gcaacactat tctcgaacag tggcaatatc
      901 ccgcaggcac cttcggtatt gaatttcgct acggctaatc atgcatccca caggcccgca
      961 tctcgggcct gatgttctgt ttcgagagtc caaaatgaaa gtgacattaa cgttcaacga
     1021 acaacgtcgt gcggcgtatc gtcagcaagg gttatggggc gatgcttcgc tggccgatta
     1081 ctggcagcag accgctcgtg cgatgccaga caaaattgcc gtggtcgata atcatggtgc
     1141 aacgtacacc tatagcgcgc tcgatcacgc cgcgagctgt ctggcaaact ggatgttagc
     1201 gaagggtatt gaatcaggcg atcgcatcgc atttcaactg ccaggctggt gtgaatttac
     1261 cgttatctat cttgcctgcc tgaaaaccgg tgcggtttcc gtgccgctgt taccttcctg
     1321 gcgggaagca gaactggtgt gggtgctcaa taagtgtcag gcaaaaatgt tctttgcacc
     1381 gacgttgttt aaacaaacgc gtccggtaga tttaatcctg ccgctgcaaa atcagcttcc
     1441 acaactacaa caaattgtcg gcgtggacaa actggctccc gccacctctt ccctctcatt
     1501 aagtcagatt ctcgccgaca atactcctct gaccacggcg ataacggtcc acggcgatga
     1561 attagccgct gtgctattta cctccggaac cgagggtctg ccaaagggtg tgatgctaac
     1621 gcataacaat attctcgcca gtgagcgggc ttattgcgcg cggctaaatc tgacctggca
     1681 ggatgtcttt atgatgcctg cgccacttgg tcacgcaacg ggctttctgc atggcgtaac
     1741 agcacctttt ttgattggtg ctcgcagcgt gttgttagat attttcactc ctgctgcgtg
     1801 tctcgcgctg cttgagcagc agcgttgcac ctgtatgctc ggcgcaacgc cgtttgtcta
     1861 tgatcttttg aatttactag agaaacagcc cgcagacctt tcagcgctgc gtttctttct
     1921 ttgcggcggt accacaatcc ccaaaaaagt ggcgcgtgaa tgccagcagc gcggcattaa
     1981 attattaagt gtttatggtt ccacagaaag ttcgccgcat gcggtggtga atctcgacga
     2041 tcctttgccg cgctttatgc acaccgacgg ttacgctgcc gcaggtgtag agattaaagt
     2101 ggtcgatgac gcacgcaaga ctttaccgcc aggttgcgaa
//
LOCUS       DAWDRC010000649         2136 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18213_length_2136_cov_2.384911, whole genome shotgun sequence.
ACCESSION   DAWDRC010000649 DAWDRC010000000
VERSION     DAWDRC010000649.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18213_length_2136_cov_2.384911"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          563 a          480 c          512 g          581 t
ORIGIN      
        1 ttgtcaaacc agcgttttcc ttcttcgacc tgttgcgaat cgagcaccag cgtaaagccg
       61 gagtagctgg cttttcctga cggtatagca tcgctcatca tgatgtcgct tcctgcaatg
      121 cgcacgttgg catgcgcgat ggcggtatcg ggaaattgca ttccggaagg gcagttctcg
      181 gcgctgtcct gcgctgattt cggcatttcg ccgaagctga ttttatagag cagttccgcg
      241 cccaacgtac gttgataata ggcaatcgcg tcggaacagt taccggcaaa agagaggtag
      301 ggacttaacg gcatgatggt gacctcagtt aagagaagcc cgttaagtgt agttcgaaat
      361 ttatacagat gagagggaag cctgataaga cgcggtcagc gtcgcatcag gcagtcgcca
      421 ccgttgccgg atgcggcgcg agcgccttat ccggcctacc gatcacaata caatcagttc
      481 tttttcacaa actcagattt cagtttcatc ggaccaaaac cgtcgatttt gcaatcgatg
      541 ttatggtcgc cttcaaccag gcggatgttt ttcactttgg tgccaatttt cagcatcgaa
      601 gagctacctt tcaccttcag atctttaatg atggtcacgc tgtcgccgtc agccagcaga
      661 ttgccgttag catctttaac gatcagctcg tcgctttcct gtgcaggttc tgcgtcgttc
      721 cattcgtagg cacattccgg gcagatgtac atgccgttat cttcgtaggt gtattcggag
      781 ttgcattttg ggcagtgtgg taatgacatg tggatttcct caaaagtcag cagggcaaaa
      841 gcgccaaaaa acggcagatt gccgaaaaag gccgcaatta tacacaaaat ccctgagttt
      901 gtcgggggta ctgctgattt tagacgacga tcgttttcat ttgaaaggaa acgcaaaaag
      961 aaaataatct ttttgcctcc atttattatt taagataagg ttaatgctct acatgttctt
     1021 tttttaattc aaatgatatt ttttatcttt ctgacgtttt aaacgtatcg gaagacgtta
     1081 tttattctaa taaaaaacaa cactctaaat ataaaattat ttcttgcagt aattataaaa
     1141 gccgaaaaca gaactcaaaa acaatctggc taaataaaag taataaaatt tgctttaagg
     1201 aagaatattc tatgtacaca cagaccctgt atgagttaag tcaggaggct gaacgcctgt
     1261 tacagctttc tcgccaacag ttgcagttac tggaaaaaat acctctctct gtacccggag
     1321 acgatgcgcc acaactggct ttaccctgga gtcagcctaa tatcgccgaa cgtcacgcga
     1381 tgctgaataa tgagttgcgt aaaatttccc ggctggaaat ggtgctggcg attgtcggta
     1441 ccatgaaagc agggaaatca accaccatta atgccattgt tggtacggag gttctgccta
     1501 atcgcaatcg cccaatgact gcgctgccaa cgcttattcg ccatatgcca gggcaaaaag
     1561 aaccggtact gcatttttca catgtcgcgc caatcgacca tttaattcaa caattacaac
     1621 agcgactgtg tgattgcgat attaagcatc tgaccgatgt gctggaaata gataaagata
     1681 tgcgtgcgct tatgcagcgg atcgaaaatg gcatcgcttt cgaaaaatat tatctgggtg
     1741 ctcaacctat ttttcattgt ctgaaaagtt tgaatgattt ggtgcgactg gcgaaggcgc
     1801 tggacgtcga ttttcctttt tctgcttacg ccgccattga gcatattccc gtgattgaag
     1861 tggagtttgt ccatctggcg gggctggaga gttatcccgg tcagttgacg ttactggata
     1921 cccccgggcc aaatgaagcc gggcaaccgc atctgcaaaa aatgcttaac cagcagctgg
     1981 cacgcgcctc ggcggtactg gcggtgctgg attatacgca actgaaatcg atctccgatg
     2041 aagaggtccg tgaggcaatt ttggcggtgg ggcaatcggt gccgttgtat gtgctggtca
     2101 ataagttcga tcaacaggat cgtaacagtg acgacg
//
LOCUS       DAWDRC010000650         2133 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18235_length_2133_cov_2.473051, whole genome shotgun sequence.
ACCESSION   DAWDRC010000650 DAWDRC010000000
VERSION     DAWDRC010000650.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18235_length_2133_cov_2.473051"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          486 a          577 c          518 g          552 t
ORIGIN      
        1 gcgtattcag cacttcctct gtgagtcacg ttgcaatcta tttgggagat aacaacgttg
       61 ccgaagcgac aggcgctggc gtccagattg tttcccttaa aaaagccata aagcatagtg
      121 ataagctttt cgtcttacga gtcccggatc ttaccccgca acaagccaca gaaatcaccg
      181 cgttcgccaa caaaattaaa gatagcggtt acaactatcg cggcattgtc gaatttattc
      241 catttatggt gactcgccag atgtgctcac taaatccgtt ctccgaggat tttcgtcaac
      301 agtgcgtcag cggcctggcg aaagcgcagt taagcagtgt gggcgaagga gacaaaaagt
      361 cgtggttttg ttcggaattt gtcactgatg catttgccaa agccgggcat ccgcttacgc
      421 tggcgcaatc aggctggatt agccctgccg atctgatgca tatgcgtact ggcgatatct
      481 ctgcgtttaa gccagaaacg cagttacagt atgttgggca catgaagcct gggatttata
      541 tcaaggcagg ccgctttgtt gggctgactc agtgataaaa gccagcctcg tgcgcaaaat
      601 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatt gtgctaattc aaaatattgc
      661 attgtatgct ttaggcctga taagcgcagc gcatgaggca atatttcatt tgtttctgtc
      721 tcaagacggg ttacagcccc aaactactgc aatcctggtt tgggtaaaat ttacctgccg
      781 tatcgggcgc taatactttg gtcggcgcac cggtatccgg attagcctca agcgtaaagt
      841 gaccttctac tgacagtaac accggttttt cactgttacc gcgcgcagcc aggtagccac
      901 gctccagctc tgcgttattc gccaccatga aacgttttcc ggtcgcgcaa tcagtgaagg
      961 tcgccgcatc agccatataa aaatacatgc cccgcagggt catcggcgtc atgggtaagc
     1021 tggattgtgc cggttccagc gtgtagttga actgcgattc aatcggatta ccttcacgat
     1081 cgagcatctc cagcgcatct cctttcgcgc gataatatga cttttcacct ttgctatcgg
     1141 ttaataccag cttgtcagcg gttcgcgccc atgtaccgta ggaagcgaag gaggaaggtt
     1201 cttcacgagc ccccagataa cgctcattca tcacccaggt tccgtctttt tcgaggaaca
     1261 gagaggtttc gattccttcg caatcagcac acggcagcac gccacgccaa ctttgcggca
     1321 tcggtttcag ttcggcagcc tgcgccggag aaagcgtatc gacttcggcc cgattattac
     1381 atcccatcag agtaaagagg ctgattacag ccatcgctgt cactatcgct tttttcacca
     1441 tccattcctt ctttttattc ccgaccgctg cgcactttgc cgcgcatcgc cttcacgctt
     1501 gatttttgtg ctttcgatgc cagcctgcgc tcttttgatg cacgggtggg ccgcgtaggt
     1561 cgtcgggctt tttgttctgt tgttaaatct ttaatcactg ccaccagccg ggccagcgcc
     1621 gcttcgcggt tcagttcctg actgcggtat tcctgtgcct taatgacaat cactccgtca
     1681 ctgctgatca aatgatggct ggcggcgagc agacgctctt tgtaatactc tggcaggctg
     1741 gacgcccgaa tgtcaaaacg cagatgaata gccgttgagg tcttattaac atgctgcccg
     1801 cccgcgccct gcgcacgaat ggcggtgatc tcaaactcgc catcagggat agcaacatgt
     1861 cgggaaatca caatcatgaa ggttgttgcc agacggttaa gttcacttcc agattattct
     1921 tatcatccga taaccagata acaccatcct gaatcgttgc ctgcagcgtc atagtacgat
     1981 cggcaaaggc gctcactttc gccagctgct cgtcgtccag ataccagacg gaaagattgg
     2041 caaactgcgc acatttgctc tgattttgct gccaccagat ttgcgccgcc cgactattat
     2101 aggcaaacag cgccacttct gcggcctggg tgc
//
LOCUS       DAWDRC010000651         2116 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18376_length_2116_cov_2.407569, whole genome shotgun sequence.
ACCESSION   DAWDRC010000651 DAWDRC010000000
VERSION     DAWDRC010000651.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18376_length_2116_cov_2.407569"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1736..1745
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          512 a          517 c          524 g          553 t
ORIGIN      
        1 tttgtgggga tatgcgcggg atttggcggt ggtttaacca ccatgccgct cgtcacgtta
       61 atgctccccg taaaaatggc aactccgctc tcggtgattg tcggcaccgc gaccgccttg
      121 tacgccacct ggctgtcacg taaagaaacg gattggcgat cggcaatggt actgattatt
      181 ttctcgtttc tgggaatacc cgtcggttta tatgcgctgt cgtatttacc cgaccatttt
      241 atgaaagtgg gcttaggcgg attccttatc ctgtactcct tttacagtat gtttattcca
      301 cgcctgccga tttatgaccg tagctggatc gccgcaccta tcggcgccgt ggcaggagcg
      361 ttaggggcag cattttcaac caacggaccg ccgatggtta tctacggtat gctgcgtaac
      421 ttaggcccgg cggcatttcg cggcactctg aacgctttct tcaccgcaaa caacatcgcg
      481 gtgatcggcg ggctggcgac cagcggaatt ttgactgtct ctatctttaa actggtgctg
      541 ttttgtattc ccaccatgat cctcggttca ctggttggac aatatgtgca taaacgcatt
      601 tcggtcaaag tgttccgtgt gatggtcttt ttattgctga ttgcctccgg ttctatgttg
      661 ataaaaggcg cgctgggaat tcccgccgct gccgcacttc ttcctccgtt taccttgtta
      721 gttatactgc aactggcatt tggtaaaaaa gtggcggcaa tccgtcgggc ggaagaaagt
      781 aaataatcat tatgcgggtt ttaatttata cccgcacaca ttctttaatt aatgaaacct
      841 gtaataacag gatggcatga aatattagtt ttatatctat ggagattatt tatggatacg
      901 ccacgcccac agctattaga ttttcaattt catcagaata acgacagttt tactctacgt
      961 tttcaagacc gccttatttt aatccatagc aaagataatc cttgtttatg gattggttca
     1021 ggtatagcgg atatcgatat gttccgcggt aatttcagca ttaaagataa attgcaggag
     1081 aaaattgcgc ttaccgacgc catcgtcagc cagtcaccgg atggctggtt aatccatttc
     1141 agccgtggtt ctgacattag cgccacgctg aatatctctg ccgacgatca tggccgttta
     1201 ttgctggaac tacaaaacga caaccttaac cacaaccgca tctggctgcg ccttgccgca
     1261 cagccggaag atcatatcta cggctgcggc gaacaattct cctactttga tctgcgcggc
     1321 aaaccgttcc cgctgtggac cagtgaacaa ggcgttggtc gcaacaaaca aacctatgtc
     1381 acctggcagg ccgactgcaa agagaatgcg ggaggggatt actactggac cttctttcca
     1441 caacccacgt ttgttagcac gcagaagtat tactgccatg ttgataacag ttgctatatg
     1501 aacttcgact ttagcgcgcc ggaataccat gaactggcgc tgtgggaaga caaagcaaca
     1561 ctgcgttttg aatgtgctga tacatacatc tccctgctgg aaaaattaac cgcgctgctg
     1621 ggacgccagc cagaactgcc cgactggatt tatgacggag taacgctcgg cattcagggc
     1681 ggcactgagg tgtgccagaa gaaactggac accatgcgta acgcgggcgt taaggnnnnn
     1741 nnnnntattc gtatgacctc ttttggcaaa cgcgtaatgt ggaactggaa gtggaacagc
     1801 gaaaactacc cgcaactgga ttcacgtatc aagcagtgga acaaagaggg cgtacagttc
     1861 cttgcctata tcaacccgta tgttgccagc gataaagacc tctgcgaaga agcggcaaag
     1921 cgcggctatc tggcaaaaga tgccgccggc ggcgattatc tggtggagtt tggcgagttc
     1981 tacggcggcg ttgtcgatct cactaatccg gaagcctacg cctggttcaa ggaagtgatc
     2041 aaaaagaaca tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg
     2101 cccaccgaca cttacc
//
LOCUS       DAWDRC010000652         2112 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18415_length_2112_cov_2.717550, whole genome shotgun sequence.
ACCESSION   DAWDRC010000652 DAWDRC010000000
VERSION     DAWDRC010000652.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18415_length_2112_cov_2.717550"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    916..925
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          484 a          500 c          499 g          619 t
ORIGIN      
        1 tatactgcgg ggttaaaaaa acatctggcc attgatttgt tggctcaaaa acttccggct
       61 gcatctgttc tgacgcttga cagttttatc caaattatta ttacggtctt tgcggttatt
      121 ttcatgatct atggcgggaa tattgttgtt gaaaaagcac aacatgttgg acaaatctct
      181 ccagtattaa aatggccaat ggataaagta tattgggtta tgcctgtcag tgggcttatt
      241 ctggtctggt attcagtaat gaatattatt gacaactatc gtaaacggaa ttttcattaa
      301 cgagggaaat atggactttg aatatatcta ccccgtctta attttatttg gcagttttgc
      361 cgtcatgctg gcaataggtg tgccaattac ttttgcgatt ggtctttctt cgctgttatc
      421 tattattact gccttaccac ccgatgccgc catttctgtg atttcgcaaa agatgactgt
      481 ggggctggat ggctttacgc tattagccat tcccttcttc gtgttagccg gaaacattat
      541 gaataccggt ggtatagcca gacgactggt taacctggcg caagcattag ttgggcgtct
      601 tcctggctca ctggctcatt gtaatatcct cgcgaataca ctgtttggtg cgatttcagg
      661 ttcagccgtt gcgtcagccg cagcggtagg tgggattatg tcaccactgc aagaaaaaga
      721 gggatatgat ccggcgtttt cagcagcggt taatattgcc tctgccccca ttggcctgat
      781 gatcccaccg agcaatgtgt tgattgttta ttccctcgcc agtggcggga cttctgtcgc
      841 tgcgttgttc ttagctggat atctgccggg catcctcacc gcgatgctga tggccgcgct
      901 ggcagcattc gcggannnnn nnnnncctag cctgatgctt atttttatca ttattggcgg
      961 tattatcgcg ggggtattta cgcctacaga agcatcggca attgcggtaa tttatagttt
     1021 agccctggcg atgatttacc gggaaatcac tcttaagaag ctcaatgata ttctgttaga
     1081 ctccgtagta accagttcaa ttgttctgtt actggtaggc tgctcgatgg ggatgtcatg
     1141 ggccatgacg aatgctgatg ttcctgagtt gatcaacgaa ctgattaccc gcgtttcgga
     1201 taacaaatgg gtcattcttt ttattatcaa tatcattctg ttgatcgtcg gtacctttat
     1261 ggatatcacg ccggcgatcc tgatatttac acctattttc ctgccgatcg cccagcatct
     1321 ggggattgat ccgattcact tcggcatcat tatggttttc aacttaacca ttggcctttg
     1381 cacaccacct gtcggcacca ttctgtttgt ggggtgcagc attggtaagg tcagtatcga
     1441 cagggcaata aaaccattac tgccgatgtt tctggcattg tttgtagtaa tggcaattat
     1501 ttgttatttc ccgcagctta gtctgatgct gccaggatta ttttcgacct gatacacaat
     1561 gaagactgca tactgagtaa ttttcaatgc cccaccactc tgtggggcat tgaattggtc
     1621 cattacggta ccggattgac caacagttgc ccaatcgagc cacggtccgc catttccagc
     1681 gtctgactgt tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc
     1741 agctccactt gtccgtcaac ccaaacggta tctttccagc ctctgtcttc cgggaacggc
     1801 atcgcaccat tcacgttacg gatctggaac atcacgcctt caatatggaa cgcttgcggc
     1861 tcgtccgcgc gcaccgtcca gcgttcccac gttccttgct gcgcagtaac atcaatacgg
     1921 ttgacgtccc acaactgccc attaatcccc gggtcatcac ccagactgat atcacgactt
     1981 cgaatcggcg aaccggccat gatttcagtt ggcagcaagc gcatcggaag actgtcagtg
     2041 accagcggca gaaggccggt tgggcgcagc gttagcacca gcgtagaaac cagaatgctg
     2101 gatggctcaa ag
//
LOCUS       DAWDRC010000653         2108 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18466_length_2108_cov_2.348758, whole genome shotgun sequence.
ACCESSION   DAWDRC010000653 DAWDRC010000000
VERSION     DAWDRC010000653.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18466_length_2108_cov_2.348758"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    252..261
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          521 a          568 c          533 g          476 t
ORIGIN      
        1 atcatcctgt cagggagagg gatctcaatt atcaatgctt aattacgtca acattttgat
       61 gacatgaaac cggagcattt tgcatggaat tttcgttttc acccaaacgt cttgttgttg
      121 ctgtcgccgc cgctcttcca ctcatggcca gcgcagcaga taccccgtca accgccaccg
      181 cacgcaaagg ctttgccgga tacgatcacc caaaccagta tctggttaaa ccggcgacca
      241 ctattgccga cnnnnnnnnn ntgatgccgg tgatgcagca tcctgcacaa gacaaagaaa
      301 cccagcagaa actggcggaa cttgagagaa aaaccggtaa gaaaccgaac gtggttgttt
      361 tcttgctgga tgatgtgggc tggatggacg ttggctttaa cggcggcggc gtggcggtgg
      421 gtaaccctac accagatatc gacgccgttg ccagccaggg gctgatttta acttcggcgt
      481 attctcaacc gagttcttcc ccaacccgcg ccacgattct caccggacaa tactccatcc
      541 accacggcat tctgatgccg ccaatgtacg ggcaaccggg cggtctgcaa gggttaacca
      601 cgctgccgca gttgctgcac gatcagggct acgtcaccca ggctatcggg aaatggcata
      661 tgggggaaaa caaagagtcg cagccgcaga acgttggctt tgatgatttc cgcggcttta
      721 actcggtatc tgatatgtat accgaatggc gtgatgttca tgtcaacccg gaagtcgccc
      781 tgagcccgga tcgttctgaa tacatccaaa aactgccgtt tagcaaagat gacgttcatg
      841 ccgtgcgcgg cggtgaacag gaagccattg ccgacattac gccgaaatat atggaagatc
      901 tggatcaacg ctggatggaa tatggcgtta agttcctcga caagatggcg aagagcgata
      961 agcctttctt cctctattac ggcactcgcg gctgtcactt cgataactac ccgaacacca
     1021 aatatgcggg cagctctccg gcgcgcacct cttacggcga ctgcatggtg gagatgaacg
     1081 atatcttcgc caatctgtat aaagcactgg agaaaaacgg tcagcttgat aacacgctga
     1141 ttgtgttcac ctctgataac ggtccggaag ccgaagttcc gccgcacggt cgcacgccgt
     1201 tccgcggtgc gaaaggttct acatgggaag gcggcgttcg tgtgccaacc ttcgtttact
     1261 ggaaaggcat gatccaaccg cgtaaatctg acggcattgt cgatctggcg gatctcttcc
     1321 ctacggcgct ggatctggcg gggcatcctg gggcgaaagt agcgaattta gtaccgaaaa
     1381 ccacctttat cgatggtgtg gaccagacat ctttcttcct gggaacaaat ggtcagtcta
     1441 accgtaaggc cgagcactac ttcctcaacg gcaaactttc tgctgtgcgt atggatgagt
     1501 tcaagtatca catcctgatt cagcaacctt acgcttatac ccagagcgga tatcagggtg
     1561 gattcaccgg cacagtaatg caaacggcgg gatcgtcggt gtttaacctg tacaccgatc
     1621 cgcaggaaag cgactctatc ggcgtgcgcc atatcccaat gggtgtaccg ctacagaccg
     1681 aaatgcatgc gtatatggag atcctgaaaa aatatccacc acgcgcgcag attaaatctg
     1741 attaagccgg cgttatcgct gagatctacc tttgccggat gcgatgctga cgcatccgac
     1801 aatcaaccgc aggcggccac aaaatggctg cttttttact tattcaccac ctgcaaatac
     1861 gcctgcataa tgtcgctggc cggacgaccg tgcgccagca aatcagccat tgctttaaga
     1921 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagcccgggt
     1981 ttgccgctgg catcgagcat aaagcggtgt ttcggacagc ctccccagca cgcttttaac
     2041 acgttacaac tgcgacactg cgccggtaac tgcttaaatt tatcttcacc aaacaccttc
     2101 tgttgcgg
//
LOCUS       DAWDRC010000654         2107 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18486_length_2107_cov_1.820175, whole genome shotgun sequence.
ACCESSION   DAWDRC010000654 DAWDRC010000000
VERSION     DAWDRC010000654.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18486_length_2107_cov_1.820175"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1625..1634
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          531 a          549 c          589 g          428 t
ORIGIN      
        1 acaaaaaaaa cgggtttatt ggcggataga aaaaaacgaa agcacaaata atgggagcgt
       61 caatttttcg agtttgctga cccgggagtg agtcttgttc cactttgcca ataacgccgt
      121 ccataatcag ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag
      181 tggcttaatc gcctgcgtgc gaatccgaaa attccattga ttgttgccgg ttccgcggca
      241 gtggcggtca tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc
      301 agcaatcttt ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt
      361 ccttaccgct tcagcgaagc cagcggcgct attgaagttc cggcagataa agttcacgag
      421 ctgcgcctgc gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaacta
      481 ctcgatcagg aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg
      541 ctggaaggcg agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta
      601 cacttggcaa tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg
      661 gtgacggtaa atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgctattgta
      721 catctggttt ccagcgcggt tgctggtctg ccgccgggaa acgtcacgct ggtggatcag
      781 ggcgggcatc tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcagttg
      841 aaatatgcca gcgatgtcga aggccgtatt cagcggcgta ttgaagcgat cctgtcgcct
      901 attgttggta acggtaatat tcacgcccag gtctcggcgc aactggactt cgccagtaaa
      961 gaacaaacgg aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca
     1021 cgccagctta atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg
     1081 ttgtcgaatc aaccggcacc tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa
     1141 aataaccgcc agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg
     1201 aatgaaacca gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc
     1261 gatgtgcaac gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa
     1321 ccattgcctc tcagcaacga acagatgaag caaattgaag ctctgacccg cgaggcgatg
     1381 ggcttttctg aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt caatagcagt
     1441 gacgaaagcg gcggagcact gccgttctgg caacagcaag tgtttatcga tcagttgctg
     1501 gctgccggtc gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta
     1561 cgcccgcagc taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc
     1621 cgcgnnnnnn nnnnatgcgg tggaagtccg tctgagcaaa gacgaacaac tccaacaacg
     1681 gcgcgctaac caacgactgg gggcagaagt catgagccag cgtatccgtg aaatgtctga
     1741 taacgatccg cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta
     1801 acctgacagg caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg
     1861 cagaggtgtt caagcacctc tcccagcgcg aagtgcaatg gcgaacgtca cgcagatctc
     1921 caacaagcag ctaaccgatg tgctggcgga gtttgagcaa gaagctgaac agtttgctgc
     1981 actgaatatc aacgccaacg attatctgcg ttcggtattg gtcaaagctc tgggtgaaga
     2041 acgtgccgcc agcctgctgg aagatattct cgaaacccgc gataccgcca gcggtattga
     2101 aacgctc
//
LOCUS       DAWDRC010000655         2106 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18499_length_2106_cov_1.642126, whole genome shotgun sequence.
ACCESSION   DAWDRC010000655 DAWDRC010000000
VERSION     DAWDRC010000655.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18499_length_2106_cov_1.642126"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    575..584
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    808..817
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          556 a          480 c          529 g          521 t
ORIGIN      
        1 tggcggcgta accccaggta aaggcggtac tacccacctc ggcctgccgg tgttcaacac
       61 cgtgcgtgaa gctgttgctg ctactggcgc taccgcttct gttatctacg taccagcacc
      121 gttctgcaaa gactccattc tggaagccat cgatgcaggc atcaaactga ttatcaccat
      181 cactgaaggc atcccgacgc tggatatgct gactgtgaaa gtgaaactgg acgaagctgg
      241 cgttcgtatg atcggcccga actgcccagg cgttatcact ccgggtgaat gcaaaatcgg
      301 tatccagcct ggtcacattc acaaaccggg taaagtgggt atcgtttccc gttccggtac
      361 actgacctat gaagcagtta aacagaccac ggattacggt ttcggtcagt cgacctgtgt
      421 cggtatcggc ggcgacccga tccctggctc taactttatc gacatcctcg aaatgttcga
      481 aaaagatccg cagaccgaag cgatcgtgat gatcggtgag atcggcggta gcgctgaaga
      541 agaagcagcg gcgtacatca aagagcacgt taccnnnnnn nnnncaagcc agttgtaggt
      601 tacatcgctg gtgtgactgc gccgaaaggc aaacgtatgg gtcacgcagg tgcgatcatt
      661 gccggtggta aagggactgc ggatgagaaa ttcgctgctc tggaagccgc aggcgtgaaa
      721 accgttcgca gcctggcgga tatcggtgaa gcactgaaaa ctgttctgaa ataaatatct
      781 gtaataagaa atagccctcg ccgcttcnnn nnnnnnnagg gctgtcggtt tcgacatggt
      841 tggccatctt atgatggcct tttttattcg ggcactaatc tgaaaagttt gcccactgct
      901 tttctcccat cttctgcgca ataatgcccg gcaaaatatc ccctctccgt tctgtaaaag
      961 catttataca agaagaaaat tgttctgccg gttgctctgg ccctgatggg tgtaaattcg
     1021 gacgtttatg cggccgatgt aaatatcgat attttaagtg cgacagtgaa agataaacgc
     1081 attgagggag tatcggtgac gctgcaacgc aatggcgcac aatctgtttc tggaaccaca
     1141 aatgcatcgg gcagcgttaa tctgggttct acatttgctg atgatcaaga tgctttattg
     1201 atcgtcaaaa aagagggcta ttctaatctg gtggtcaaat gttcgtgtgc tggcatgact
     1261 tatgcgatta gtccggcaat gactagtctg gatggcatgc gtgtagtttt gagctggggc
     1321 gaaaagcctt ttgatcttga ttcacatttg atcttccctg gcggacatat ctattttgat
     1381 agtaaagagg ggactgatgc taatctggat gttgatgata cggatagcta tggatcagaa
     1441 actgtcacta tcagcaaaaa gcattttggc gaaagttaca tctatgccgt gcaggactat
     1501 agcaacaaag ggttgcccaa cagtaattat ctctctgcca gtaaggcaaa agtgtttgtt
     1561 tatgtaggga gctccctggt gcgtagttat agtgtccctg ctggaaaacg agggaacatc
     1621 tggaccgtgt ttaaactcaa tccaaacggc gagtttgaag atatcaactc tgttaccagc
     1681 gccaatttta acgacaccac attaggcgtc agggatttag ccactgtgat aatgcctgcg
     1741 acgggttctt caactccggc ttcacctgcg atgcaaaaca gtggagatac tcaactggca
     1801 agaaaatata atcgtgaagg cgaagctgtt tacaaaaccg gtcaacttga gcaagccatt
     1861 caactcttcc aacaggcaac agaattagac ggcaattacg gacaggcatt tagtaacctg
     1921 gggctggcgt atcagaaaaa tggcaacatc gcggaagcaa tttgggcaaa tcgtaaagcg
     1981 atctcgttag ccagtggagc aaatgcggcg acaacgcgcg ctaactccta ttataacatt
     2041 gccaaaattt atgaaactgc tggacaggac gctgatgcat tgcagcatta tcaactcgct
     2101 cttcac
//
LOCUS       DAWDRC010000656         2099 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18570_length_2099_cov_2.261742, whole genome shotgun sequence.
ACCESSION   DAWDRC010000656 DAWDRC010000000
VERSION     DAWDRC010000656.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18570_length_2099_cov_2.261742"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1630..1639
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          535 a          529 c          522 g          503 t
ORIGIN      
        1 acctatatat atttcaattt acgaggtttt aattctactt ctttaaaacc gcgtcaaaat
       61 aaaacaatag gatattaatc tttttttgtg tttatgtgcc ttgagtcgcc agtattcata
      121 actattcctt acatcgacga atgataattt gtaggatagc gaactgtatt tttctttctg
      181 cgagttaacg cattgccttt ttgggcaaat aacgcgcttt tggttttttg aggaatagta
      241 atgacgaaac atgccaggtt tttcctcctg ccctccttta ttctgatctc cgcggcttta
      301 atcgccggtt gtaacgataa gggagaagag aaagctcacg tcggtgaacc gcaggttacc
      361 gttcatattg tgaaaacggc cccgttagaa gttaagactg aattaccagg ccgcaccaat
      421 gcttaccgta tagccgaagt tcgcccacag gttagcggga tcgtactgaa tcgcaatttc
      481 actgaaggca gcgatgtgca agcaggccag tccctgtacc agatcgatcc cgcgacctat
      541 caggcaagtt atgacagcgc gaaaggcgaa ctagcgaaaa gtgaagccgc cgccgccatc
      601 gcgcatttga cggtaaaacg ttacgttccg ctcgtgggta cgaaatacat cagtcagcag
      661 gagtacgacc aggccattgc tgatgctcgc caggccgatg ccaccgtgat tgccgcaaaa
      721 gccacagttg aaagcgctcg catcaatctc gcttatacca aagtgactgc gcctattagc
      781 ggacgtatcg gcaaatcgac tgtgaccgaa ggcgctcttg tcactaatgg gcaaacgact
      841 gaactggcga ctgtccagca gctcgatcct atctacgttg atgtgaccca atccagcaat
      901 gattttatga ggctgaagca atccgtagag caaggaaatt tgcataagga aaacgccacc
      961 agcaacgtag agttggtcat ggagaacggt caaacctatc ccctgaaagg tacgctgcaa
     1021 ttctccgatg tgaccgttga tgaaagcacc ggctccataa ccctacgtgc tgtcttccct
     1081 aacccgcaac atacgctttt gccgggtatg tttgtgcgcg cacggattga tgaaggcgtc
     1141 caacctgacg ccattcttat cccgcaacaa ggcgttagcc gcacaccacg tggtgatgca
     1201 accgtgctga ttgttaacaa taaaagtcag gttgaagcgc gccctgtcgt tgccagccag
     1261 gcgattggcg ataaatggtt gattagcgaa gggctgaaat ctggcgatca agtcattgtc
     1321 agcggcctgc aaaaagcgcg tccgggagag caggttaaag ccactaccga tacccccgca
     1381 gatactgcat cgaagtaagg caatctgaca tggcaaactt ttttattcga cgaccgatat
     1441 ttgcatgggt gctggccatt attctgatga tggcgggcgc actggcgatc ctacaattgc
     1501 ccgtcgccca gtatccaacc attgcgccgc ctgcggtttc tgtttcggca aactatccgg
     1561 gtgctgatgc gcagaccgtg caggatacgg tgacgcaggt tatcgaacag aatatgaacg
     1621 gtatcgatan nnnnnnnnnc ctgatgtata tgtcctccac cagcgattcc gccggtagcg
     1681 tgacaattac ccttaccttc cagtccggga ccgatcctga tatcgcgcaa gtgcaggtgc
     1741 agaacaaact ccagctcgcc acgccgttgc tgccgcagga ggttcagcag caggggatca
     1801 gtgttgaaaa gtccagtagc agctatttga tggtggcggg ctttgtctct gataacccag
     1861 gcaccacaca ggacgatatc tcggactatg tggcctctaa cgttaaagat acgcttagcc
     1921 gtctgaatgg cgtcggtgac gtacagcttt tcggcgcaca gtatgcgatg cgtatctggc
     1981 tggaccccga tctgctaaac aaatataaac tgacaccggt tgatgtgatt aaccagttga
     2041 aggtacagaa cgatcagatc gctgccggac agtttggcag aaccccagcg ttaccaggg
//
LOCUS       DAWDRC010000657         2095 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18611_length_2095_cov_2.132353, whole genome shotgun sequence.
ACCESSION   DAWDRC010000657 DAWDRC010000000
VERSION     DAWDRC010000657.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18611_length_2095_cov_2.132353"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    660..669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          440 a          554 c          570 g          521 t
ORIGIN      
        1 cgcctttgcg ctggtgtggc actggctggc aagcgcctat tttatcgtgc tgtttttctt
       61 ttcgttgttc gatccgggca acagcctgaa atttatgatg ggtgcaacgg tgcgcagcct
      121 ggcgattatt ggtatcgcag catttgtttc cggtatgttt tcccgctggc tggcgaaaac
      181 catcaccctc tcgccacata ctcagcgaaa ttatccggag ctgcaaaaac ggctgaatgg
      241 ctggctttcg gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg cggtaatgtt
      301 gctgttgagc gcatggggat tgttcgattt ctggaactgg ctgcaaaacg gtgcggggca
      361 gaaaaccgta gatatcctga tccgtatcgc actcattctt ttcttctcgg cggttggctg
      421 gacggtgctc gccagtttga tagaaaaccg gctggcttcg gatattcatg gccgcccgct
      481 acccagcgcc cgtacgcgaa ccctgctgac gctgttccgt aacgcgctgg cggtgattat
      541 cagtaccatc accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg cgccattgct
      601 ggcaggtgcc ggggcattag gtctggctat ctcgtttggt tcgcaaacgc tggtgaaagn
      661 nnnnnnnnnt taacaccggg gtatttattc agtttgaaaa cggcatgaac actggagatt
      721 tggtgactat cgggccgttg accggcactg tggaacggat gtcgattcgc tccgtgggcg
      781 tgcgccagga taccggggcg tatcacatca ttccgtggtc ttcgataacc acctttgcta
      841 acttcgtccg cggtattggt tcggtagtgg caaactacga tgttgatcgc catgaagatg
      901 ctgataaagc caatcaggca ctgaaagatg cggtagcaga attgatggaa aacgaagaaa
      961 ttcgcgggct ggttattggt gaaccgaact ttgccgggat tgtcggctta agcaataccg
     1021 cgtttacact gcgtgtttcg ttcaccacgc tgccactcaa acagtggacg gtccgctttg
     1081 ccctcgacag ccaggtgaaa aaacatttcg acctggcggg tgttcgcgcg ccggtacaga
     1141 cttatcaggt gctgccagct ccgggcgcgg ccccggctga accgttgccg ccgggggagc
     1201 caacgcttta acgctggcga ttgacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc
     1261 aggctataaa gcggctctgc ttttgccctt gggccatctc tttttttacc accttcaccg
     1321 cacctacgtc cgtcaaagca cgatacaacg gcggtaagtt ttcaccacga gataccagcg
     1381 aggtaaacca catcacctgc ttcgcgaagc ctttgctctc ttcaatcatc tttttaataa
     1441 aggcgacttc accgccttca caccacaact cctgttgctg accaccaaag ttcagtgcat
     1501 cgtctttgtt cagccccagg ttacggcgtt tacgttcact acctgcccgt gccgcagtgg
     1561 cggaatcgtg gaatggcggg ttacacaagg tcgcatcgta ttgctcgttt ttatggatga
     1621 tgccgttaaa aatcgcccca ctctcttttt gccgacgcag acgaatggcg cggttaagcc
     1681 ccggattagc actgatgatt gcctgcgcac tgcttaacgc ctggctgctg gtttcactac
     1741 cggtaaaacg ccagccatat tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa
     1801 cgccgatatc cagaatgctg gcattagctg gaattgttcc gcttgcttcg gccagtaaat
     1861 cggcaaggtg atgaatataa tccgcccggc ccggtactgg tgggcagaga aaaccgtccg
     1921 ggatatccca gttcgctacg gcgtaaaaat gggccagcaa cgcattattg agcgccttca
     1981 ccgccagcgg attggcaaag tctacgcttt gctccccggc gggtgtaagc gtgaggaatt
     2041 gcctgagttc aggattgacc tgacaaagcg tggagagatc gtagcggctg tgatg
//
LOCUS       DAWDRC010000658         2090 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18659_length_2090_cov_2.334152, whole genome shotgun sequence.
ACCESSION   DAWDRC010000658 DAWDRC010000000
VERSION     DAWDRC010000658.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18659_length_2090_cov_2.334152"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1844..1853
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          502 a          538 c          543 g          497 t
ORIGIN      
        1 atcgaagcca gtatgtcttt cccactggtg aaagagcgtc tgaactggaa taccaatgcc
       61 acatggatga tcacttcgga gcaaaaagac accggtaatc ctctgtcggt catcccgaaa
      121 tatactatca ataactcgct taactggacc atcacccagg cgttttctgc cagcttcaac
      181 tggacgttat atggcagaca aaaaccgcgt actcatgcgg aaacccgcag tgaagatact
      241 ggcggtctgt caggtaaaga gctgggcgct tattcactgg tggggacgaa cttcaattac
      301 gatattaata aaaatctgcg tcttaatgtc ggcgtcagta atatcctcaa taaacagatc
      361 ttccgatctt ctgaaggggc gaatacctat aacgagccag gccgggctta ttatgccgga
      421 gttaccgcat cattctgatc gtagtgtttc aggcgcactg cccgcgtaaa agcgggcagt
      481 gtttagtggc ttaactcatg acaacctgct gtgtaatttg cgttttcacc actgatatct
      541 aacagtgcct ggcgaaagga ggcattgaac atcggcccgt gtcccaggtt ggggaaatcc
      601 caaaatacgg cattgacgcc tttatctttc agtatagtga gggtggtatg aattttcgac
      661 agcaccccga cagcatgcgt ttcccggtta tcaccctgtg tcgccgagcc ttccattatc
      721 gccaggtgtt tggtgcagaa ttgcagaggc tcaaccgccg taacgcggct tagcaaagca
      781 tcataacctc tgcccaacga cgggctggcg ctgtagtacg accggaagta agaggaggac
      841 agccaggaat ccagcacgaa gaggccgccg taggagtgcc cccataagcc gcggcgttgc
      901 cgatcgatat tcagtccctg ttccactttt ggggcaatac gcgtttccag taactggcgg
      961 aagttgttgc tgccaccact cttacggcta aaacgccctg agtggagatc tgtttttctg
     1021 ctttctgctg ctggcgtata gtcgtaagcc ctgctgttga gatcgaaagg gaggttggtc
     1081 tgatacccga cagccacgat cactggcggt gttttttctg acaattgttt gagcagttcg
     1141 tcatccaggc ggtccataac tgcgttaccg tcaagcatat ataaaatcgg gtaacccgat
     1201 gccggtgcgg ttgtattcgg cacggccgtc catacccgat aatggcgtgt gccatcgaca
     1261 aagtcgaacg acgaggcgcc gaaatggtaa aacacggagc ctttatcggc tatattcggc
     1321 cccagtggcc gcatatccgg cttcgcataa acttgcgcac tacagataag ggtgaggcaa
     1381 gaaagatgaa agaaaatcgc tttatgcggg cgtgttgagc gatactcgcg ggcatacatc
     1441 tgatgattat tccttttccg caggtgacaa tttgatttat tcaggaagtc tgtaccgttt
     1501 cctgaaagtg tttgtgggtc aactcaaccc tgtagtaaac caatcccgtc aatcagtgca
     1561 ccgcgccacc atgcgtaatc gtgaccaccg gtgtagattg cgcaatggct ttcgacgcca
     1621 gcggtaatca gccgctggtg aagttgctga acgtgaggaa ctgtcgaacc ttccagcgat
     1681 cccacgcaca ggctgatacg cacgccctgc ggtggggcag aaagcagatg ctcactcacc
     1741 catgaggtat cggtttcgct gaacaagcct ggtcgactgg ttctttctgg cgtccaccac
     1801 attgaaggag agtggctgag caccagaccg aacgtttccg gtgnnnnnnn nnnatccttt
     1861 atcagcttgc tgcgcccgcc gagtatctca gtgcgttcat gttcattaat gttgtcaatg
     1921 cccagtacag ccacgggcac gatgcgcctg ttatttatgg cggcgtcaat tgccgcgcat
     1981 acgccaaggt gatcaaacca ggtttcacca tcgggcagca cgaccagccc gaggggctga
     2041 gaaatatcaa tgtccggaat atagagccga acctggcgag atttcccggc
//
LOCUS       DAWDRC010000659         2075 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18799_length_2075_cov_2.367822, whole genome shotgun sequence.
ACCESSION   DAWDRC010000659 DAWDRC010000000
VERSION     DAWDRC010000659.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18799_length_2075_cov_2.367822"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          576 a          478 c          501 g          520 t
ORIGIN      
        1 cctgtctcgt tttgcgccgt atctggcaaa agcatttcct gaaacggctg ccgcgggggg
       61 gattattgaa tcagaactgg ttgctattcc tgctatgcaa aaacggctgg aaaaggaata
      121 tcaacaaccg atcgccgggc aattgctact aaaaaaagac agccatttgc cgatttccgg
      181 ctccataaaa gcacgtggcg ggatttatga agtcctggcc catgctgaaa aactggctct
      241 ggaagcgggt ttgctgacac ttgaggatga ttacagcaaa ctgctttccc cggagtttaa
      301 acagttcttt agccaataca gtattgctgt gggatcaacc ggaaatctgg ggttatcaat
      361 tggtattatg agcgcccgca tcggctttaa agtaacggtg catatgtctg ctgatgcccg
      421 cgcctggaaa aaagcgaaac tgcgcagtca tggcgttact gtcgtggaat acgagcaaga
      481 ttatggtgtt gctgtcgagg aaggacgtaa agcatcgcag tctgacccga actgtttctt
      541 tattgatgac gagaattccc gcacgttgtt ccttgggtat tccgtcgctg gccagcgtct
      601 taaagcgcaa tttgcccagc aaggccgtat cgtcgatgct gataaccctc tatttgtcta
      661 tctgccgtgt ggtgttggcg gtggtcctgg tggcgtcgca ttcggactta aactggcgtt
      721 tggcgatcat gttcactgct tttttgccga accaacacac tccccgtgta tgttattagg
      781 cgtccatacc ggattacacg atcagatttc tgttcaggat attggcatcg acaaccttac
      841 cgctgccgat ggccttgcag ttggtcgcgc atcaggcttt gtcgggcggg caatggagcg
      901 tctactggat ggcttctata cccttagcga tcaaaccatg tatgacatgc ttagctggct
      961 ggcgcaggaa gaaggtattc gtcttgaacc atcggcactg gcgggtatgg ccgggcctca
     1021 gcgcgtgtgc gcatcagtaa gttaccaaca gatgcacggt ttcagcgcag aacaactgcg
     1081 taatgccact catctggtgt gggcgacggg aggtggaatg gtgccggaag aagagatgaa
     1141 tcaatatctg gcaaaaggcc gttaataacg tttcaacgca gcatcgccat cctttccctg
     1201 ggtgagcgat gctgcggatg acgcagactt aagattctgc cgttttacct gctatagcct
     1261 cctctttttc catcacccaa cgcctttcgc tgtaaacggc ggtttcgcaa accaaaccaa
     1321 aaccgtaagt aagataaaag caacagccgc catacggaaa atctcatttg cagaaataga
     1381 aagtgactgc tgagttattt cattattaat ttcattaaga actcctgaaa gcgaaccata
     1441 gtatttatcc ataatttgtg acgatgaatt aaacacgggg ttaaattgat cgatggttgc
     1501 tgtcaactga ctatggtgta acgattcgcg gcgtccccac agtgtcattg tcaacgacgt
     1561 accaactgat cctgacaagg tgcgaaaaaa attactcata ctcgaggcat tggcaaattt
     1621 attatctggc aagcctgaaa acgaaatcgt tgttaagggt aaaaagaaac aggcaacggc
     1681 gaatccctga aaaaactgtg gcaaaatgat gcctgtaaaa tcaatcgttg gcataaatgt
     1741 cacagaacgc cagtaatagc aaaccgcata catcaaaaaa ctaaatgtca ccaacaaccg
     1801 catatcaatt ttgttgccat aacgtcctat caaaggtgat attaatagcg acatgatacc
     1861 gatgggcgca taggcaagtc cggcccatat cgcattatac cccatcgttt cctggagtaa
     1921 ctgcggcata agaactatcg ctccagagta aaataaatac gcgcatgtga tactcacaat
     1981 accaatggtg aaattacggg acttaaacaa actgaggtca agaatcggat tctctgaggt
     2041 cgactcccaa atgactaaag agatcagaga aataa
//
LOCUS       DAWDRC010000660         2067 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18880_length_2067_cov_2.438370, whole genome shotgun sequence.
ACCESSION   DAWDRC010000660 DAWDRC010000000
VERSION     DAWDRC010000660.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18880_length_2067_cov_2.438370"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          563 a          576 c          502 g          426 t
ORIGIN      
        1 gctaatggga ttcgaaccca cgacaactgg aatcacaatc cagggctcta ccaactgagc
       61 tatagccacc actacaaatc ttgttacgcg gtattactac caccgcagct caagcgccgg
      121 gactaaatgg cgcgcccgac aggattcgaa cctgagacct ctgcctccgg agggcagcgc
      181 tctatccagc tgagctacgg gcgcttagcg ccgttgcggg ggtggataat acggacttcc
      241 cacccctctg tctagtccct ttttaaataa attgcgcgtt tggttacggt ttgtgcgttt
      301 tgctgcttat tcctccagtt tatgcgcttt accgtggcga ttaagcccaa aaactttata
      361 aatcgccgtc accgtcagca taaagatgat gccgacaaac agcgacatac gcgtatcttc
      421 attaaagtac atgccgatca aaacgcaaat caggaatgcc attgttacgt agttggccca
      481 agggaacagg attgagcgga acggatggct ggcaattgct gctttatgcg cacgacgaaa
      541 acgcagctgg cttatcaata tcacaaacca tggcaccatc cccggaagca cgctggcact
      601 gtagacgtaa acaaacacac gctgcggatt gggaatgatg tagttcaggc atgagccaat
      661 tagcagaata gcaatagata ctgccacacc cgcaaccggt acgccgtgac gggaaacttt
      721 cgccattgcc gccggtaact gacggttttt cgccagtgcg tagagcatac gtccgcaact
      781 gtacatgccg ctgttacagc cagagagcgc agccgtcagc accacaaagt tgataatgcc
      841 cgccgctgcg gtaatgccga ttttggcgaa agtcagtacg aacgggctac cgttgctgcc
      901 tatttcattc cacgggaaga tagtaacgat aacgaaaatc gcgccaacgt agaaaatcag
      961 gattcgccac agcaccttgc ctacggcact gcgcagcgtc acctgcggat tcttcgcttc
     1021 accggcagta atgccaatca gctccacgcc ctggtaggac gccaccacaa tacacaatgc
     1081 ggtcaggaac cctttccagc caccagcaaa gaaaccgcca tgctctgtga gattgctaaa
     1141 accaatcgac tgcccgccat tgccaaagcc aaagaaaatc acgcccaggc caatgacaat
     1201 catcacgata atcgtggtga ctttgatcat cgcgaaccag aactcgattt cgccgtacaa
     1261 cctcaccgca gccagattcg ccaacgccac cagcgccacc gcgatcaatg cgggtatcca
     1321 ctgcgccatc tccgggaacc agaactggac ataaacgcca atggcggtga tttctgagat
     1381 ccccaccgcc atccacataa accagtaaga ccaggcggtg agataaccaa agaacgggct
     1441 catataacga tgcgcgtaaa cggcgaacga accggtaacc ggttcgagga acaacatttc
     1501 gcccattgaa cgcatgatga aaaagacgaa cagcccggcg atgatatagg ccaacaatac
     1561 ggatggcccg gcccatttca gggtactggc ggcccccata aacaggccga cgccaatggt
     1621 gccccccagg gcgatgagtt cgatatgtcg agcttccagc ccacgctgta gctctggttt
     1681 gttatctgcc ataaatcctc gtgttgtgtt tgcatgcttt ccggtgttac cggttatcgt
     1741 tatgggtaca tcgagtgttg cgaatgtttt cgtaattcag gagaaatggc aaataaagca
     1801 ttaaaaattt gaatgctttg tgtaataaaa aagcagacag gcgacgaagt gaccactccg
     1861 tcgctttaca aagagaggaa aatcagaagt tgccggtgta atgccagcgt aaataacgca
     1921 gcaaacgaag ctgacgctta atgcggctcg gctgcgaaag caggcggtag agccactcca
     1981 gccccagcgt ttgccagatt ttcggtgcgc gttttacgtg accggtgaaa acatcgtagg
     2041 tcccgccaac gcccatatac agcgcat
//
LOCUS       DAWDRC010000661         2064 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18903_length_2064_cov_2.197611, whole genome shotgun sequence.
ACCESSION   DAWDRC010000661 DAWDRC010000000
VERSION     DAWDRC010000661.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18903_length_2064_cov_2.197611"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    684..693
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          481 a          550 c          534 g          489 t
ORIGIN      
        1 gggcagtctc ccactccggc ttcatacgac aatcgctttt cagcaaaaca caattactct
       61 ttatcgccca gcagaacgga ttccagcgcg attttgatca tgtcgttgaa ggtggtctga
      121 cgctcagcgg cagtggtctg ctcgtgagtg cggatgtggt cagacacggt gcagatggtc
      181 agggctttcg cgccaaattc tgcagcgacg ccgtagatac cagcagcttc catttccacg
      241 ccgaggatgc cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg
      301 tcagcggaga acaggttacc aacacgagca tcaacgccca gtgcttttgc cgcgtctacc
      361 gcgttacgca ccatgtcgaa gtcagcgata gcggcaaagt catggtcttt aaaacggatg
      421 cggttaactt tggaatcggt gcaggcaccc ataccgataa cgacgtcgcg cagttttacg
      481 tgcggcagaa cagcgccaca ggaaccaacg cggataattt tcttcacgcc gaaatcagtg
      541 atcagttctt tggtgtagat ggagcaggac gggataccca taccgtggcc cattacggaa
      601 attttgcggc ctttgtaagt accagtgaag cccagcatac cgcgaacgtt gttcacttca
      661 cgggcatctt caaggaaagt ttcnnnnnnn nnnaaaatcg cccatttctg cattaatgtg
      721 tggagtagcc atcgttttat cctttttgtg acatattaaa ggcggagtca ctgctccgcc
      781 caaatccatc agaacatggc tttgccatat tccatatcag aagtaccaaa atattttgcc
      841 agagtctggc cgatatccgc gaaggtttca cgatgaccca gcgaacctgg tttcactttc
      901 gggccgtaaa ccagtaccgg aatgtgttca cgcgtgtggt cagtaccggt ccaggtcggg
      961 tcacaaccgt ggtcagcagt gagaatcaga atatcgtcat cgcgcagcaa agacatcagc
     1021 tccggcagac ggcggtcgaa cagttccaga cccgcagcat aaccggcgac gtcgcgacgg
     1081 tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct
     1141 tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca ggccggtcgc tttcactttt
     1201 ttggtgatac cgcagttggc gtagatgtct gcaattttac cgaccgaaac cacctggccg
     1261 tgtttttcat caaccagttt ctgcagcacg gtcggtgctg gcggctcaac agccaggtcg
     1321 tgacggttac cagtacgctg gaagttacca gctttgtcgc cgataaacgg acgagcgata
     1381 acacgaccga tgttgtagcc gccgttggtc agctcttcac gggcgatttc gcacagttcg
     1441 tagagtttat ccagaccaaa ggtttcttcg tggcaggcaa tctggaacac ggagtcagcg
     1501 gaggtataga aaatcggctt gccggttttc atgtgctctt cgcccagttg atccagaatg
     1561 accgtaccgg aagagtggca gttaccgagg taacccggca gattagcgcg ttcaaccagt
     1621 ttatccagca gctcttgcgg gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac
     1681 agaaccggga caccggcgat ttcccagtga ccagacgggg tgtctttacc ggatgacatt
     1741 tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc catccattcc cgccggaatg
     1801 aaaccggtag aaccttcgtg tgctttcgcc agcccaagac gggtcagatt tggcagattg
     1861 agcgggcctt tacgaccgtg gtcagcttcg cctttggcac aagcttctgc gatatgaccc
     1921 agggtgtcag ccccgacgtc accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat
     1981 gagtccagca ccataataaa tgcacgtttc atatgttctc cgtactcagt gcttcaaatt
     2041 taaatgatca gatcagtata ccgt
//
LOCUS       DAWDRC010000662         2063 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_18916_length_2063_cov_1.984064, whole genome shotgun sequence.
ACCESSION   DAWDRC010000662 DAWDRC010000000
VERSION     DAWDRC010000662.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18916_length_2063_cov_1.984064"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          500 a          526 c          527 g          510 t
ORIGIN      
        1 cgcaagatga tcatgatcga taattacatc gcgtacaccg gcagcatgaa tatggtagac
       61 cctcgctact tcaaacaaga tgcgggcgta gggcaatgga ttgacctgat ggcgcgtatg
      121 gaaggcccca tcgccaccgc gatggggatt atttattcct gcgactggga gattgaaacc
      181 gggaaacgta ttctgccgcc gccaccagat gtcaatatta tgccgtttga gcaggccagc
      241 ggtcacacca ttcacacaat tgcttctggc cccggctttc cggaagatct cattcaccag
      301 gcattattga ctgctgctta ttcggctcgt gaatatttga tcatgacaac gccctatttt
      361 gtgccaagcg atgatttact tcatgcgatt tgcacggcgg cgcagcgcgg ggtggatgtc
      421 agtattatcc ttccgcgaaa aaatgactcg atgctggtcg gctgggccag tcgcgcattc
      481 tttacagaac tgctggctgc tggggttaaa atttatcagt ttgaaggcgg gttactgcat
      541 accaagagcg tgctggtcga tggtgaacta agtctggttg gcaccgttaa ccttgatatg
      601 cgtagtctgt ggctaaattt cgagattacc ctggcaatcg acgataaagg ttttggtgca
      661 gacctcgccg ccgttcagga cgattatatt tcgcgttctc gtctgctcga tgcccgttta
      721 tggctaaaac gtccattatg gcaacgtgtc gccgagcgac tgttttactt cttcagcccg
      781 ttgctgtaaa acgtcgccaa cagaggttaa acaggtagtg actatggata tggatctaaa
      841 caatcgcctg actgaagatg aaacgcttga gcaggcctac gatatttttc tcgaactggc
      901 tgccgacaat ctcgatccag ctgatgttct gctgttcaat cttcagtttg aagagcgcgg
      961 cggcgcggat ttattcgatc cggcggaaga ctggcaggaa catgttgatt tcgatttgaa
     1021 cccggacttt tttgccgaag tggtgattgg tctggcggac agtgaagatg gtgaaatcaa
     1081 cgatgttttc gcgcgcattc tgttatgccg cgaaaaagat cacaaacttt gccatatcat
     1141 ctggcgggaa taaataatcg cttttggcgt gaataagggc tgacaactgt cagcccttat
     1201 tgcttttaaa gcggatcgac tttcaggcaa gaaacggcgt gtctgaaact cccttccagc
     1261 acaggacgtg ttttggcgca ctccggaccg gcaatcggac aacgggtacg gaaaacacaa
     1321 ccggaaggcg ggttgatcgg cgacggtaat tccccttcca gtaactggat ggttttgttc
     1381 ttctccagat ccggatcagg tatggggact gccgacatca atgccctggt gtaaggatgt
     1441 agcggattgt ggtagacctc atcataggtc cccagttcta ccgcatggcc gagatacatc
     1501 accagcacac gatcggaaat atgttttacc acggccaggt cgtgagcgat aaaaattaat
     1561 gacaatccca tctcacgttg caactgctgg agcaggttga ccacctgcgc ctgaattgac
     1621 acgtccagcg ccgacaccgg ctcatcacag ataatcagct tcggttcaag aataagagca
     1681 cgagcaatcc cgatacgcct gcaccgccca ccggagaact catgcggata gcggttaatc
     1741 aggttaggca acaaaccgac tttcagcatc atcgccttca cgcgctcgcg aacttcctgg
     1801 cgtgacattt tcggatgata agtacgcagt ggttcagcga tgatctcgcc gatggtcata
     1861 cgcgggttca gtgatgccaa cggatcctgg aaaatcatct gaatatcact gcgtacggca
     1921 cgccattcat cgggcttcat gcccagcaac tctttaccta accaggcaac atgaccgtcg
     1981 gtcgctttaa ccaggccaat gatggcgcga gcaaaggtgg acttaccaca tcccgactcc
     2041 cctaccacac ctaatgtttc ccc
//
LOCUS       DAWDRC010000663         2053 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19017_length_2053_cov_2.341842, whole genome shotgun sequence.
ACCESSION   DAWDRC010000663 DAWDRC010000000
VERSION     DAWDRC010000663.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19017_length_2053_cov_2.341842"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          526 a          460 c          475 g          592 t
ORIGIN      
        1 gtcagttcaa ccgctttggt ttctgcatcc tgcgctgcgg cgatcaacgc cacttgttta
       61 gcacgatcgg cttcggcagt ctggcgagtg gtttctacgt tttgttgggc gcttactgct
      121 tctgcgagtg caaggttagc acgcgcttct gcctgggact gttgttccga tttggcggca
      181 atagcgatcg atttcgtctg gttagcgatt tcggtgacct gctgctgttc gatctcttta
      241 atgcgaactt cacgttcggc ctcaaccttt cttgagcgga cggcctgttc gcggtcgatt
      301 tccgtttcct gaatctgtcg ttcagccaga attcgcgtct gctctgcttc acgacgacgt
      361 tcagcttcaa aagccgcaat tttcgcattc tgctcagcgg tacgggtttt aacctgctgc
      421 tcctgctcaa gcgtcataaa cgcttcttgt tgttcaatct ccaacttgcg cgaaagtgca
      481 tcacggtttt tctcacgcac tgcgacttcc acatcctgtt caacttcgtt acgttcgcga
      541 cgacggcgct ccgtctcctg agtcagtttg gttaaacctt cggcgtcaaa ggcattgttc
      601 ggattgaaat gttctttcga ggtctggtta aagttggtaa gtgaaacgct ttccagttcc
      661 aggccatttt tcgacagatc ttcagctacg gtattttgta ctccctgcac gaagttctcg
      721 cgggtatcct gtaactcatg catggtcatt tgtgccgctg ttgcacggag ggcatcgacg
      781 aatttatcct cgaccagcat acgtaaatct tctggtgata gagtgcgttg ccccagcgtc
      841 tgggccgcag tggcgatgcc ttctactgac ggttttaccc gtacaaagaa agccactaca
      901 acatcaacac gcatacgatc tttcgtaatc aggctatcaa tagtagaacg gctgacttcc
      961 agcttcaggg tattcatatt gatgggaatt atttcatgaa agatcggcat cacgattgcg
     1021 ccaccgctca tcaccacttt ttgcccactt aaaccagtac gaacaaatgc ttgctctgcc
     1081 gaagcgcgac gatagagcct ggcgaaaata attccaataa taaacagaat gcatacggcg
     1141 ataatcgcgg taaacatcca ggagggcaca gaattaacga tatcatccat tattaaattt
     1201 ccttttgatt aataaatcac aatatctggg gccagggatt attttccgcc aaatatcgcg
     1261 ttgcagaaag tcggcaaata attaacactt tgtctccttt agtgaaagtt tttccttctt
     1321 caggctccag tagcaaataa tgtatttggc caaattgatc ggtgagtttt ccttcgcaag
     1381 ggttacctga agtggcttga tggccggtaa ttaatgccat gctaccaaca tattcttctt
     1441 ctgtaatagc tgaactatgg tcgcgtggaa tccagggtgc aacgacttta ccggtataat
     1501 gcactgcaat aattgtaaac agtaaactga caggaaccac gaacaggttg gggagtggtg
     1561 attgccagag cgtaacgcag gcatgttgca acaggatgcc aataagacca aaaaaaccag
     1621 cgagtaagca gaggacgacg agagctggta atcttccaat attgagataa tgaagtgcct
     1681 ggctaatatg acctgtggtt atggaatcat aatggtcaag atgtgcatca agagcaccgg
     1741 agagcatgtg accgcagata agtgcaaaaa tttctaaaag gccgatcaag agtacaaagg
     1801 aaatagcgaa aagataaggg gtgttatagt cggcgaataa aatcatatgt cactccaatg
     1861 tccatttagt gacgcaatat ttactttgga atagtttcct tgcttacatt tatctttgtt
     1921 aataataaca catgttagag aggttctgga agagaaagtc ttttaatatg gtttttttat
     1981 ttttcactaa aaagtgtgat cggggacaat atatttacgc acgttatgtt taaaggcact
     2041 acactgattg ggg
//
LOCUS       DAWDRC010000664         2048 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19064_length_2048_cov_2.191169, whole genome shotgun sequence.
ACCESSION   DAWDRC010000664 DAWDRC010000000
VERSION     DAWDRC010000664.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19064_length_2048_cov_2.191169"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    981..990
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1244..1253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          596 a          446 c          461 g          525 t
ORIGIN      
        1 ttgagtggaa tgtccatctc accagttgca gatgaacaga atgttttttt atcttttacc
       61 agcacaaacg tacgaacatt catgctaaac gccgcgcggg cggtcagttc ggggttagcc
      121 tgctggcagg tatctaaggt cagcggctgg agccggtcag tagtggattt aaggtcggca
      181 aaataggtat cgagatactt ttgtacgtcg gtaatcagtg tgtcgtattt gacttcgtgc
      241 ttatgccagg ccactaaaaa ctgaaggcag ctcaccagta tcgcaatcat cacgcctgca
      301 acaatgcagg taagcaggag cttacgtcca gaattgggag cgcgtataaa catgattccg
      361 ttaatttcct gaaattacga caacatttaa aagataacaa tgaccggact atagcctgga
      421 tcaagacgcg gaaacatcat tttctctgat gaatgaaaac aaaaaagcac tgcctaagca
      481 gtgctttcag ttaactcgtc aggatagcca agggattgca tccaaacggt ttattagctg
      541 cggctgagaa cccggcgtgc ttcgttgtaa cgcttcttcc agtatggttc attcatgctg
      601 gaaataataa caccactgct ggtggaagca tggacaaact ggttgttgcc gatataaata
      661 ccgacatggc gtcccgttga accggcacgg aacagaacta aatcacccgt acgcaaatta
      721 ctgcgggaaa cagatttacc catttcctgc tgttcataag tcgaacgcgg aagttctaag
      781 ccaaattgct cacggaatgt acgctgtacg aaaccagaac aatcgatacc ttttttagtg
      841 ctgccgccca gacgatagcg tacgcctttc cagtcagcat actgatccat aattcgcgat
      901 tttacgtcga cattacgaac caggttttca aattcatcct gagaagcttg cagtgatgat
      961 gtttcactac ccactgcacg nnnnnnnnnn ctgcacgtgt ctcaggatgc atattctttg
     1021 cggtgttatt tgcactacat gcagaaagca gaaccgctac tgcaatcgcg gggatcccgc
     1081 gcaagatata tctcaaaatc ggttgagatt tgaccatgtt gtttgttttc ccttgaagtc
     1141 cttaacgaca aatatcgtta taaaaaaatg ccaagcgaca cgagactaat tacctgggca
     1201 tgttgaaaca attccttaag gccaaatctg tggactaacg cacnnnnnnn nnncgcacaa
     1261 aattatatgc tgtgaggata atctaccgcc gaggcaaaac gagttcgagg ttacccgatt
     1321 ggtgcggtct tggcgagcgg ttttacatga ttttttataa gaaaaagtga tacagaaagt
     1381 taatcagcgg gattggtcaa ttaaagtacg attgagtgaa acgcatgcaa tccattcatt
     1441 tacttagaga atgatttttg cgctcgatgg atgaaagcag gaggcgcatc atcaggatta
     1501 tcctcactat aaaaataaac ctgatgatgt taattactgt gagttatttg ttttggaaat
     1561 gtttgttttt tccaggtagt gatttgtcaa aaaaggtgat taatcgatca cttaatggcg
     1621 tcagcaacac ccagggcaag ccgatcaata tcacggtcat ggaaccgacg atgatgtcag
     1681 taaaccagtg tgcgccaatc attactctgg gaaacgcaaa aatcacaaaa ataataaggg
     1741 cgataaggcc tgcaactttg ccgaaataac gccacatgaa tgccgaaaaa ataagcagca
     1801 tcataccgtg atcgccggga aagctatctc gtgaggcatc tttcgtggga acagagagca
     1861 gttcgctgac gcggttaata ttggtaaaag tcaacgttgg gctggcccgt tttacaggaa
     1921 ttagtgcctg acccagctgg tttaatacca ctgcagttaa tagcatgacc agaccaataa
     1981 tcacgatacg tcgtctgcct ggggcgtttt ctttcagcca gaaactcagc atcaacattc
     2041 ccatcgcc
//
LOCUS       DAWDRC010000665         2047 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19075_length_2047_cov_2.289659, whole genome shotgun sequence.
ACCESSION   DAWDRC010000665 DAWDRC010000000
VERSION     DAWDRC010000665.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19075_length_2047_cov_2.289659"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1331..1340
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          534 a          544 c          454 g          505 t
ORIGIN      
        1 gcagatcggt agccggtccg gtgtaatcat ccgctttagt gctgttagga tctttgccga
       61 gataattcag cacggtagca aacacttctt ctggcgcatc caggaaagag acaccgcagc
      121 ttttcagttt ttccagattt tcaggtttga ggatcaagtc ccagctatcg acgggcgcgt
      181 tttcgcccag caccgcttta actttatcga cgttatagcc aatcccggtc gtcgcccaca
      241 tatagggcat agcaaattta ttgtcgggat cgtgtttggc gaccaacttc agcagctccg
      301 gatcgagatt cttccactcc ggcaatttgc ttttatccag cggctggaaa actcccgctg
      361 tcagctggcg ctcaagaaag cttgctgacg gcaccaccag atcaaagccg gtgctccccg
      421 ccattaattt gccttccagt acttcgttag aatcgaaaac atcgtagacg actttaatac
      481 cggtgtcttt ttcaaagttg gccaccgtgt ccggggcgat ataatcagac cagttataaa
      541 tgtggagtgt tttttgttca gccgcgagcg tgctgacaga gacggccatc agagcccccg
      601 caaccagacc cgatagccat tttttattta aggcggtcat atctctttcc ttctgaaaat
      661 tcgttaacaa acgaacaaaa gttgtcacac tgaaagatat gcaataaacg tgcatatttt
      721 tatcggcgcg gtgtaagaaa agagaactct ctcccggcct gcactgctca ctatataaaa
      781 gcatcgcaag aataacttta gccagggttt gagactatag ctcggattaa aaaacggtat
      841 ttaacaaatt tttatgcgta tttgctctat gtgataaagc caaatcggca caagagaagt
      901 gaattaatct ttaataaaag gttaaaaaca gcggataatt ttgtggcacg cagtcactgc
      961 ggcagcgact gcgtgctcga aaggagttat cagtggagga aatagttttc ggtaacgtta
     1021 ttttgccctt catcgtcagt cggtaacagc agttgatggg cattagcttc aagaatgacc
     1081 atcgaaatct gctcttcgct ctgacgcacg aaccatgcaa actgctcata ggttacgccc
     1141 tgcataacgg ataaagactg gcaaaccacc agctttggca gattatcatc ctgcatatca
     1201 agaaatgctt tcacggtcag cgaactggca ttgatggctg ataaatccgc cgccagcggc
     1261 aatacggctg aaggtcggac ttccgccatg gcagaaaaca ggatcgtgtt atcgatcaga
     1321 tcgattttag nnnnnnnnnn caaagacacc gtcgaaattc tgcatatggg gcagatgcag
     1381 agcctgacag ttatcacatt caaaaaaact catccccagg tcatcgagcc attgacgcaa
     1441 cgtatccaga ccaggaacga ccagcgatgt cataattttg tacaacctct tccgataaaa
     1501 agaccggcac agcttacgca aaaagcgcag gcaaaaccat gatcagtaat gtgattgcga
     1561 ctaaccaccc gttttcaggc aatattctgt cgtagcgtgg cgttcgatcc agcggatcat
     1621 tttacccgcg atgtcgatac cggtggtttt ttctattcct tccagccctg gcgacgcatt
     1681 cacttccatc accaacggcc cgcgattagc acgcagaata tccacaccag caacgtccag
     1741 cgccatcgtt cgcgcagctt ttatcgcgat ttcacgctcc tgtggtgtga tactggcgac
     1801 gcttgccgcg ccgccacgat gcaaattgga acgaaaatcg ccctctttcg cccgccgttc
     1861 aatcgcagcg actacttcat cgccaacaac cagacagcgg atatcgcacc cttgcgcctc
     1921 tttgatatat tcctgcacca gaatatgcgc gttcagaccg cggaaagcgt caatcacgct
     1981 ttccgccgcc tgacgcgtct ccgccagcac gacgccaatt ccctgcgtgc cttcaaccag
     2041 cttgacc
//
LOCUS       DAWDRC010000666         2041 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19142_length_2041_cov_2.201410, whole genome shotgun sequence.
ACCESSION   DAWDRC010000666 DAWDRC010000000
VERSION     DAWDRC010000666.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19142_length_2041_cov_2.201410"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          573 a          445 c          460 g          563 t
ORIGIN      
        1 ccttttaata ccagaaaaac tgataataat gcacagggga acgatattat aatagcgatt
       61 atcaatgcat aattcataaa tttaaattgt aatgattaga ggggaaaatg tcagcacatt
      121 tgcgaaatga atcaaaaagc ccgcagcaat gtgcgggcgt tagtgtcagc gcacaaccag
      181 cacggagcac tctgcgtgac gcactacagc tgcggcgtta gaaccgagca gataagtggt
      241 gatatccggt cgatgggaag caatgatgat catatgagcg gggatcttct tcgccaattc
      301 cagaatgcgg tctttgggcg agccttcttc aacatggaca tgcactctgt cggttggcag
      361 tttaaatttt ttaatgatct cttccagttg cgatttggct tccgctttca ggtcatccat
      421 tgccggtaat tctgcggaat aggctaaacc cagagaggca tagtagggca gcgaaggtat
      481 taccgtcagg aaatgaacct ctgcatcatc aatctttgcc tctgcctcaa cgtggctaat
      541 cacgcgttga gttaattctg aatcggaaat atcgataggg acaagaatcg ttctgttcat
      601 aaaacctcct gttttagtat ccgcataaag tgtaacgcca gatgacactt tttgtgtaat
      661 gacggagttc acatttttaa tttagatcaa aggaggaaga ataagcagaa aaagcccgcc
      721 aaaacagcgg gcagggggat ttagaactga taaaccagac ctaaagcgac gatatcatcg
      781 gtagagatgc cgttggcagc gtagaaactg tcatcttcat ccaacaggtt gattttatag
      841 tcaacatagg tggacatgtt tttattgaaa tagtaagtcg cgccaacatc ggcgtattta
      901 accagatctt tatcatcaac acctgccggg ttgtctgcac caccggcagc gtgcaggtca
      961 cggcctttag acatcaggaa agagactgcc ggacgcagac caaaatcaaa ctggtactgt
     1021 gcagtgactt caaagttctg ggttttgttt gcaacagcat aatcgctgtc gccaaacggg
     1081 gtcatattac gcgtttctga atacatggtt gccaggtaaa tattgttagc atcgtatttt
     1141 agcccagcag tccacgcgtc tgctttatca ccacctgccg cagtatggtt aacctggtca
     1201 ttggtgcggt cagaagaggt gtatgccgca ccagcgctaa agcccatgcc taaatcatat
     1261 gttgtggaaa gaccccagcc gtcaccgttt tcatggcgaa catcacgtcc gttgttggtg
     1321 ccttcctgac cattactggc accttcgttg ttaccttgat actgcaccgc gaagttcaga
     1381 ccatttacca gaccgaagaa atcagtatta cgataagtcg cgacgccatt ggcacgacca
     1441 gtcataaagt tgtctgcatt ggtataagag tcaccgccaa attcaggtag catatcagtc
     1501 cagccttcga tgtcgtacat tacgccataa ttacgtccgt aatcgaaaga accgtaatct
     1561 gcaaatttca gtccggcaaa tgccagacgg gtccatgact ggtttgttga agattcggtg
     1621 ttgtttgcct gaatattgta ttcccattga ccgtagccag tgagttgatc gttaatttgg
     1681 gtttcgcctt taaaacccag acgagcatag ctctggtcgc catctttcgc tgaattatca
     1741 gaaaaataat gcaggccatc aactttgcca tacagatcta atttgttgcc gtctttatta
     1801 taaacttcgg ctgcatgtgc agcacctgca gcgagcaggg caggaattaa gagtgccagt
     1861 actttgcttt tcattgaata aatcctttag ttattttatt tgcctttcat ccccattttg
     1921 gggagtgaaa acatcctaaa ggagaagttc agttagccga taaatatcat ttgttacgcc
     1981 ttaagtaaaa ccttaattaa ataattcttc agaaatgata ataagtctgg tgaatgtatt
     2041 g
//
LOCUS       DAWDRC010000667         2041 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19151_length_2041_cov_1.737160, whole genome shotgun sequence.
ACCESSION   DAWDRC010000667 DAWDRC010000000
VERSION     DAWDRC010000667.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19151_length_2041_cov_1.737160"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    344..353
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          440 a          592 c          532 g          467 t
ORIGIN      
        1 cgtcaaccgc tggtgcagag ttatccagcg catgagcgat ggcaaaacct aaacgcgcct
       61 gatcttcaac cggtgcgcgg taagtccacg cagcgatatc atccagacgg gtgtcatcaa
      121 cgttggtaac aaacagtgga tgcttcgcac gttgaccgat gttgaggatt gccgcaatct
      181 gccagtcagc cactttctgt gctgccgcca tttcgcgtgc tttacctttt accgcctggc
      241 gcaccgccag cgcaacgcgc gcgccggtct gggtaacgtc ttcgcccagc accagtaccg
      301 catcgtaaga ttcgatttcg cgcagagccg gagtataaat gccnnnnnnn nnncgatacc
      361 ggtatagaag ttttcttcgc ccaccagctc acgcagcgca aagttgcttt ccacgctggc
      421 acgcggagaa ccaataccga tcactttctt cgactgacgc agaatatctg ctgcaccctg
      481 cattgcctgt tcggcgttga gggtaatgaa atcatcgcca cgacgccgta ctggctgacg
      541 cggacgatct ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta
      601 gtggtttacc gtaccgttgt aacggttttc gattcgacgc agttcgccat aacgctcgcc
      661 ggggctgatg ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc
      721 ccatttacgg ttgtaacgct cggagtgcgt tttgtcggtg aatacgccgg tcgggcaaat
      781 ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta
      841 gacgttgtcg tgcgcaccgt aaacgcccag gtccgtgccg tcagcgtaat ctttgtagta
      901 acgcacgcag cggtagcagg cgatgcagcg gttcatttcg tgagagatga acggccccaa
      961 atcctgatta cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat
     1021 cacggtcata tcctgaagat ggcagttacc gccttcttca catactggac agtcgtgcgg
     1081 gtggttggtc attaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc
     1141 aatggaaata aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg
     1201 cgtgtcttcc gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc
     1261 cagcgccgga tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag
     1321 caggttgtcc gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag
     1381 catgcttcca gttttctcag tcagagactg agcgttaatc gaaattcggt taccagcgct
     1441 ctttcagcag gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga
     1501 ttcccgcctc aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac
     1561 caggtgcgtg ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg
     1621 tttcgatatc gcccggctgg ccttcgccac gctccagcgc acgcagaatt ttcacgctcc
     1681 acggcagacc gtcgcggcat ggcgtacacc agccgcagga ctcacgggcg aaaaactctt
     1741 ccaggttacg caccagcgac accatgttaa tctcgtggtc aaccgccatc gccagcgccg
     1801 tacccagacg gcttcccgct ttaccgatac tttcgaattc catcggcagg tcaaggtgcg
     1861 cttcggtcag gaagtcagtc cccgctccgc ctggctgcca ggctttgaat ttcagaccgt
     1921 cacgcatacc accggcgtag tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt
     1981 cccacagacc cggatttttc acccgaccag agaagcccat cagcttggta ccagcatctt
     2041 t
//
LOCUS       DAWDRC010000668         2038 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19179_length_2038_cov_1.748865, whole genome shotgun sequence.
ACCESSION   DAWDRC010000668 DAWDRC010000000
VERSION     DAWDRC010000668.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19179_length_2038_cov_1.748865"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          444 a          554 c          537 g          503 t
ORIGIN      
        1 gcaataaatg gcttgctgtt gcgcgggctg gcgttgtgga tagcctgggc gaaaatctct
       61 ttcccggtgc cgctctggcc gttaatcaaa acgctaacgt ctgattgtgc caccagccgc
      121 gcctgttcca gcaagcgcag catcagcggg ctgcgggtga caatcgcctc gcgccaacgt
      181 tcgtcggtgg ctggcgcgga ttgttccagc gcatcgtcaa ttgcctgata tagcgcatct
      241 ttatcgacag gcttggtgag aaaactgaac acgccctgct gtgttgcagc aacggcatca
      301 ggaatagaac catgcgcggt aagaataatt actggcattc ccggctgcac tttctggatt
      361 tcagcaaaca gctgcatacc gtccatttca tccatccgca ggtcgctgat gactaaatct
      421 actttttcgc gattcagtac ccgcaatcct tcagcaccac tttccgccgt gaccacactg
      481 tagccttcgc tggtcaggcg caggccaagc agtttcagca atcccggatc gtcatcgacc
      541 agtaataaat gcgcaggttt atggctcatc aggagtgacc tcatcggtgg atggcgcggg
      601 cttttcactc tcgtgcggcg tatccggcga gaaatttcca gccggtttgc gggtcgagag
      661 ctggcgttca atatcggtca ggttttccag cttgcgggta gtgagttcca gttgctgttg
      721 tagaacgtga tgttgctggc gcaaagtatc cagctcgctg tcgctggact gctggagttt
      781 gctgtagcgt tggcgctctt ccgccagttg cagttgcagc gcctgaccgt cgcgccaaag
      841 ttgatacagt gggcgaacct gtgccgggat ctcggtactt agcgcatcaa tgcgcgcgac
      901 cagctggcgg cgctcatacg gagtaatttt ggcgtcggcg agcaaaatcc cttgtttaaa
      961 ggtattttgc cagttgccgt cgtcatattg gcgggcttgc tgacgcgact gcgcaggcat
     1021 taaacgatca gcacaatcca tcgcccgcag ccagtaaagc ggattggttt cggtagattt
     1081 accttgcagc gcccagatgt cgctacattc agtagaaaga tagtcagcca gttgataaac
     1141 cggaattttt tcttctgctg gcgtatcaat ggctggccta ttgtgactct gcacgcaacc
     1201 cagcaatgcc agacatggca gccctgccag ccacagccgt cggggcaata atcgttgaaa
     1261 aatgtgtcgc atattcacca gacttaaagc ctatcccagt gggcgtaatt gttgcaggca
     1321 gtttggacat ggacagcgcg gagaaaccgg agcgtacatg tcgtacgtga agatttcgag
     1381 cactgcccgg gcgtactgac aagtaaaata gcctgatggg atgggctgtt agcattattt
     1441 cgtgtttttc gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac
     1501 cagatacagt tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc
     1561 gctgcccttc accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat
     1621 ggcgcgttcc tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc
     1681 accatgtaaa ctgctgcgaa ggcaaatatt accggattca gccccgtagt gcaccgcatt
     1741 ggagtaaaga ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc
     1801 tttgagatca acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac
     1861 cactgtctcc accagcggtg ctaactcaac attctccagt tccaccgcac tgtccgcctg
     1921 tttacggttg taatcaagca gttgttcgat cagcttttgc aaattgcggc tgctgctatc
     1981 aagaatgctc accacctctt tttgctctgg cgtaagcggc ccgacaacct gatcagcc
//
LOCUS       DAWDRC010000669         2033 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19222_length_2033_cov_1.517695, whole genome shotgun sequence.
ACCESSION   DAWDRC010000669 DAWDRC010000000
VERSION     DAWDRC010000669.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19222_length_2033_cov_1.517695"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    429..438
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1587..1596
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1815..1824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          468 a          534 c          516 g          485 t
ORIGIN      
        1 aatggttgaa actcgtccga aaaatagtgg ctggcatcgg cctgctcctg aatcaaccgc
       61 tttactggta cttcgctgat ttttttacgc tgcggtcggt ggacaatcgt gtcctgctta
      121 atcttgcgag tccccgccat caactggcga aacagcgcct ggtcctcctc gctaagtgtt
      181 gttttctttt tcatttatct gtctctattt ttaattttcc tacagtttac ccgactcacc
      241 gtacaatcga tcgcttaaaa cgtatttttc ctcactcacc gcgtgtgaat gctgatttat
      301 cgtcatcttc atggcaaact agccgccgaa attattgcga tcatgccctg gaggaatacg
      361 tggataaaat tttcgttgat gaagcagtaa atgagctgca aaccattcag gacatgttgc
      421 gctggtcgnn nnnnnnnnta ttgttgaacg cgtgatccgc cgcgtcaacg agcgcattcc
      481 ggtggcttac ctgaccaaca aagcctggtt ctgcggccat gaattttacg tcgatgaacg
      541 cgtgctggtg ccgcgctcgc cgattggtga actgatcaac aataaatttg ccggactcat
      601 cagcaagcaa ccgcagcata ttttagatat gtgtaccggt agcggctgca tcgccattgc
      661 ctgtgcttat gccttcccgg aagcggaagt cgacgcggtg gacatctctc cagacgcgct
      721 ggcggttgct gaacagaaca tcgaagaaca cggtttgatc cacaacgtca ttccgattcg
      781 ttccgacttg ttccgtgact tgccgaaagt gcagtacgac ctgatcgtca ccaacccgcc
      841 gtacgtcgat gcagaagata tgtccgacct gccaaacgaa taccgccacg agccggaact
      901 gggtctggca tctggcaccg acggcctgaa actgacgcgt cgcattctcg gtaacgcggc
      961 agattacctc gctgatgatg gcgtgctgat ttgtgaagtc ggcaacagca tggtacatct
     1021 tatggaacaa tatccggatg ttccgttcac ctggctggag tttgataacg gcggcgatgg
     1081 tgtgtttatg ctcaccaaag agcagcttat tgccgcacga gaacatttcg cgatttataa
     1141 agattaagta aacacgcaaa cacaacaata acggagccgt gatggctgga aacacaattg
     1201 gacaactctt tcgcgtaacc accttcggcg aatcgcacgg gctggcgctc ggctgcatcg
     1261 tcgatggtgt tccgccaggc attccgctga cggaagcgga cctgcaacat gaccttgatc
     1321 gtcgtcgccc tgggacatcg cgctatacca cccagcgccg cgagccggat caagtcaaaa
     1381 ttctctccgg tgtttttgaa ggtgttacta ctggcaccag catcggattg ttgatcgaaa
     1441 ataccgacca gcgttctcag gattatagtg cgattaagga cgttttccgt ccaggtcatg
     1501 ccgattacac ctatgaacaa aaatacggcc tgcgcgatta tcgcggcggt ggacgttctt
     1561 ccgcccgtga aaccgctatg cgcgttnnnn nnnnnnttgc caaaaaatat ctcgccgaga
     1621 aatttggcat tgagattcgc ggttgcctga cccagatggg cgacattcca ctggaaatca
     1681 aagactggtc tcaggtcgag caaaatccat tcttctgccc ggacccggac aaaatcgacg
     1741 cgttagatga actgatgcgc gcgctgaaaa aagaaggcga ctctattggt gcgaaagtcc
     1801 ccgtcgttgc cagtnnnnnn nnnnctgccg cacatgcgcg cgcgcatcgg ccggcacgag
     1861 ccggtctttg accgcctgga tgccgacatc gcccatgcgc taatgagcat taacgcggtg
     1921 aaaggcgtgg aaattggtga tggttttgac gtggtagcgc tgcgtggcag ccagaaccgc
     1981 gacgaaatca ccaaagacgg tttccagagc aaccatgcgg gcggcattct tgg
//
LOCUS       DAWDRC010000670         2032 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19231_length_2032_cov_1.945878, whole genome shotgun sequence.
ACCESSION   DAWDRC010000670 DAWDRC010000000
VERSION     DAWDRC010000670.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19231_length_2032_cov_1.945878"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1166..1175
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          475 a          572 c          470 g          505 t
ORIGIN      
        1 cgcaggcttt ttcagtcttt atcttgcagc gataagtact tacagtaatc tgtaggaaag
       61 ttaactacgg atgtacatta tggaactgac gactcgcact ttaccttcgc ggaaacatat
      121 tgcgctggtg gcacacgatc actgcaaaca aatgctgatg agctgggtgg aacggcatca
      181 accgttactg gaacaacacg tactgtatgc aacaggcact accggtaact taatttcccg
      241 cgcgaccggc atgaacgtca acgcgatgtt gagtggccca atggggggtg accagcaggt
      301 tggcgcattg atctcagaag ggaaaattga tgtattgatt ttcttctggg acccactaaa
      361 tgccgtgccg cacgatcctg acgtgaaagc cttgctgcgt ctggcaacgg tatggaacat
      421 tccggtcgcc accaacgtgg caacggcaga cttcattatc cagtcaccgc attttaacga
      481 cgcggtcgat attctgatcc ccgattatca gcgttatctc gcggaccgtc tgaagtaata
      541 ttgcacaggt ggcaaacgcc acctgtttct tacggttttc tcgccaccgg cacatccaga
      601 tttttcagta tttcgacaaa tggagaggga ttctctttgt taaacagtgc ccatacccga
      661 tggcgtgccc gggtcagcgc cacgtacatt aaccgccgtt cttcagcgtc cgggaaatcc
      721 tcaaccggtg gcagtagcgc ctcttccata atcgactccc gcgccgcagc cggaaaacca
      781 tcactcccct cctgcaagcc aacgatgatg acgtaatccg cctgttgccc tttgctggca
      841 tgaatggtca taaagtcgat ttgcaacttc ggccagcgtg ttgccgcttt ttccaggctg
      901 gcaggcctca tgtgatggta acgcgccagg atcagaatgc gctcttccgg tttggcataa
      961 ccagagagct tatccagcaa agcgtcaaga tggctctcat ccaataacgt gacggctttt
     1021 ttgtctccat tggttaagct gtttagcggc tttttcagct ggcctgggtt ctgctgaata
     1081 aaccggtttg ccacctcacc gatacgactg ttaaaacggt aagtcgtgtc taaatcgcag
     1141 cgatcacctt caccaaagtt ttcatnnnnn nnnnntttgc gcaccgctga agcgataaat
     1201 cgcctgccag tcatcaccaa cggcgaacaa cgtcgtctga ctgttttgct tgcgtaatgc
     1261 cgctaacaac gctgcgcgct gcggcgagat atcctgaaat tcatcaacca gaatatgctt
     1321 ccacgggctg ataaagcgac ctttctccag aatcacaatc gcctggtgaa taaggcccga
     1381 aaaatcgaca gcgttttctg ccttcagcgc acctttccag gcttttaata acggggccat
     1441 caacttgata cgtttactga acagatcgcg aatcacttcg ggtgcactgg caatcatttc
     1501 tgcctgtgca ccaccgtgca tccgcatcag acttacccag cgatcgaggc gagaggcaag
     1561 acgacgctgt aatttttcat catcccagaa attaccttct ggcactgacc actgcatttc
     1621 ttccgtcagc cattgccgcc agcccttcgc ctgcgctttc ttttcgctgc attgcttgca
     1681 ccactcagca ataaaaagtt catgacgggc agcggtatca ttttccagtt tgctgactat
     1741 cggaactttt ttgctaccct gctgaataat atgcagcgcc agcgcatgaa atgtgcgtgc
     1801 ggtaatgtct tcggtatgta gtcgttctcg aatacgctcg tccatctctt cagcggcttt
     1861 gcgaccaaac gccagcaata aaatttgctc aggggacgct tcaccacgcg ccagcaacca
     1921 gcctgcacgg gccaccagca ccgacgtttt tccgcttcct gcaccagcca gcactaacaa
     1981 agaatgctcg ccattaacga ctgcccaggc ctgcgccgga ttcagcggtg aa
//
LOCUS       DAWDRC010000671         2026 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19294_length_2026_cov_1.848300, whole genome shotgun sequence.
ACCESSION   DAWDRC010000671 DAWDRC010000000
VERSION     DAWDRC010000671.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19294_length_2026_cov_1.848300"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1558..1567
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          396 a          496 c          618 g          506 t
ORIGIN      
        1 ggttattgcg cgttgccccc tggtttttac cggtgggcat cgtggcggtg tggcaactgg
       61 cctcctcggt tagctggcta tcgacgcgta ttttgccctc accggaagga gtggtgatgg
      121 cgttctggac gctctctgcc agcggcgaac tgtggcagca tctggcgatc agctcctggc
      181 gtgcgctgat tggtttttca attggcggat cgctgggatt aattctgggg ctgattagcg
      241 ggctgtcgcg ctggggagaa cggctgctgg atacctccat tcagatgttg cgcaacgtgc
      301 cgcatctggc gctgctcccg ctggtgattt tgtggttcgg cattgatgaa tccgcaaaaa
      361 tatttctggt ggcgctcggt acgctattcc ccatttatat caacacctgg catggcatcc
      421 gtaatatcga ccgtgggctg gtggagatgg cacgtagcta tggattatcc ggcataccgc
      481 tgtttatcca tgtgatcctg cctggtgccc tgccctcaat tatggtcggt gtgcgttttg
      541 cgttgggcct gatgtggctg acgctgatcg ttgccgaaac tatttctgcc aattccggca
      601 ttggttatct ggcgatgaat gcgcgggagt ttctgcaaac ggacgtggtg gtagtcgcta
      661 ttattcttta cgccctgctc ggcaaacttg ctgacgtcag cgcgcagctg ttagaacgtc
      721 tctggttgcg ctggaacccg gcttatcatt tgaaggaggc cacggtatga atacggctcg
      781 tctgaaccag ggcacaccat tgttgctcaa tgcagtaagc aaacattacg cggaaaatat
      841 cgtcctgaac caactggatt tacatattcc ggcaggtcag tttgtggcgg tggtgggccg
      901 cagcggcggt ggcaaaagta ctctgctgcg cctgctggca ggtctggaaa caccaaccgc
      961 aggcgacgtg ttagcgggca ccacaccgct ggctgagatt caggatgata cgcgaatgat
     1021 gtttcaggac gcgcgtctgc tgccgtggaa atcggtgatt gataacgttg ggttaggcct
     1081 taaaggccag tggcgcgatg cggcgcgtca ggctctggct gcggtaggac tggagaatcg
     1141 cgcgggggaa tggcctgccg cactttctgg cgggcagaaa caacgtgtgg cgctggcaag
     1201 ggcgctgatt catcgaccag gtttattgtt gcttgatgaa ccactcggtg cgctggacgc
     1261 tttaacgcga ctcgagatgc aggatttgat tgtgtcgctt tggctgaagc acggttttac
     1321 cgttttgctg gtgacgcatg atgtgagcga agcggtggcg atggctgacc gggtgttgtt
     1381 aattgaagag ggaaaaattg gtcaggattt gacggtggat atcccccgtc cgcgccggtt
     1441 agggtcggta cgattagcag agctggaagc ggaagtattg cagcgggtga tgcagcgtgg
     1501 tgaatttgaa actcgcctga gaaaacaggg gtaaattctc cgaatggcgg cgctattnnn
     1561 nnnnnnncac gtaccgtctc cagcacattc gccgccgggc tggtggcttg caggataaac
     1621 catttatcca tcaccagacc atcctgatgc cacttgtcgt cgtactcctg catcagcgcg
     1681 tcacggcatg gcagttgtgc agcaaccgcc gcagaaagcg ccgccagcgc atcggtcata
     1741 ttgttcgctt cgtggaactg tttgctcacc agcacatcag ccagatgcgt ttcaccaaaa
     1801 gcgaggaagc gcaggcaggc attacgcaga gtgcgttttg caatatcttc atgctcaaca
     1861 cggtactcgc tctggtaatt cgcgttgtaa atagccagca gctcatctgc cagttcagtc
     1921 gccagagtac gagtgagtgc ttcgcgtact tcggcaatag caatcgggtc gatgatatcg
     1981 aacagttctg ccatttcatt gacggaaggc agcgtcagga tttctg
//
LOCUS       DAWDRC010000672         2023 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19327_length_2023_cov_1.807927, whole genome shotgun sequence.
ACCESSION   DAWDRC010000672 DAWDRC010000000
VERSION     DAWDRC010000672.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19327_length_2023_cov_1.807927"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1044..1053
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          448 a          630 c          527 g          408 t
ORIGIN      
        1 attcatggct tcttgcaggc tgatttcccg acgcaggctc agggaggcgc gcggtcgcgg
       61 taacactttc atcgagattt cagtgagcac gccaagacaa ccgtagcttc cggccattaa
      121 ccgtgagaga tcgtatccgg caacgttttt catcacttcg ccaccaaaac gcagatgttt
      181 tccagtgccg gtaataatgc gcgtgccgag gacaaaatcg cggaccgaac cactccacgg
      241 gcgacgcggc cccgccagcc cgcaagcgac catcccgccc caggtggctt cttcaccata
      301 atgcggcggc tcgcagggga gcatttgccc ggcgctctcc agcgctgctt caatcgccac
      361 cagcggcgtt ccggcacgcg cggttatcac cagctcggtc gggtcgtaat taacaatgcc
      421 gcgatgacaa cgcacatcca gcgtttgccc ggtgacaggg cgacctaaaa aggctttact
      481 attgctgccc tgaatcacca gcggcgtttt atcgctaatc gcctgattca cctgctccag
      541 cagcgcctgg ctgtaatcac actcgcgtag catcagaaac gctccagttc aggaaaaggt
      601 aaatgaccgt gatgcacatg catggcacca aattcagcac agcggtgtag cgtgggaatg
      661 ttttttcctg ggttcagcaa accatcgggg tcaaacgccg ccttgaccgc atggaaggtc
      721 gtgatttcat cgctgttgaa ctgggcgcac atttgattga ttttttcgcg cccgatgcca
      781 tgttccccac tgatgctgcc gccaacttca acgcagagtt cgaggatctt cccgcccagt
      841 tcttccgcgc gggcaaattc accgggttcg ttggcatcga aaaggattaa cgggtgcatg
      901 ttgccgtctc cggcatgaaa gacgttggca acgcgtaaat catattgctg cgataaacgg
      961 gcaatgcctt ccagtacgcc aggcagcgcg cggcgtggga tggtgccatc catacagtag
     1021 taatccgggg aaatgcgccc cacnnnnnnn nnntcctacc gccgggaacg catttttgcg
     1081 accggcccag aaacgtactc gctctgcttc gtcctgtgcc agacggacgt cagtcgcacc
     1141 cgcgttcaac aagatgtcgt taacctgctc gcagtcttcc tgtacgtcag actccacgcc
     1201 gtccagttcg cataacaaaa tcgcttcggc atcgacggga taaccggcat gaataaaatc
     1261 ttccgccgcg cgaatcgaca ggttatccat catctccagc ccaccgggga taatgccatt
     1321 ggcgatgatg tcaccaaccg caagtccggc tttttctacc gagtcaaagc tggctaacag
     1381 aacccgcgcc acgggcggct tcggcaacag cttcaccgtt acttcggtgg tcacgccgag
     1441 cataccttcc gatccggtaa acagcgccag caggtcaaaa ccaggcgaat ccagcgcgtc
     1501 cgatcccagc gtcagcgcct cgccatccag catttgcact tcaattttca gcaggttatg
     1561 tacggttaga ccatatttca ggcagtgaac gccaccggca ttttccgcca cattgccgcc
     1621 aatcgaacag gcgatttgtg aggacggatc cggcgcgtaa tagagattat gcggcgcaac
     1681 ggcctgggag atcgccaggt tacgcacgcc cggctgcacg cgcgcgcggc gaccgatggg
     1741 gttaatgtcg aggatctctt taaagcgcgc catcaccaac aacacacctt tttccagcgg
     1801 cagcgcgcca ccagaaagcc cggtgcctgc gccacgggtc accaccggca cacgcaggcg
     1861 gtggcagaca gccagaatcg ccgtcacctg ttccatctgc ttaggcagaa caaccagtaa
     1921 tggacgcgtg cgatacgcgc tcaacccgtc acactcgtaa ggaatgatct cctcatcggt
     1981 atgcaggatc tccagtccag ggacatgctc acgcagtgcc atc
//
LOCUS       DAWDRC010000673         2019 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19367_length_2019_cov_1.925153, whole genome shotgun sequence.
ACCESSION   DAWDRC010000673 DAWDRC010000000
VERSION     DAWDRC010000673.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19367_length_2019_cov_1.925153"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          442 a          520 c          499 g          558 t
ORIGIN      
        1 gtaaaccact gctggacgtc tggatgagcc acggcgataa agttaccgct atcccgtccg
       61 acttcgtcac cgtagctagc accgaaagct gcccgtttgc catcatggcc aacgaagaaa
      121 aacgcttcta tggcgtacag ttccacccgg aagtgactca cacccgccag ggtatgcgca
      181 tgctggagcg ttttgtgcgt gatatctgcc agtgtgaagc cctgtggacg ccagcgaaaa
      241 ttatcgacga cgctgtagcc cgcatccgcg agcaggtagg cgacgataaa gtcatcctcg
      301 gcctctctgg tggcgtggat tcctccgtta ccgcaatgct gttgcaccgc gctatcggta
      361 aaaacctgac ttgcgtattc gtcgacaacg gcctgctgcg tctcaacgaa gcagagcagg
      421 ttctggatat gtttggcgat cactttggtc tgaatattgt tcacgtaccg gcagaagatc
      481 gcttcttatc agcgctggct ggcgaaaacg atccggaagc aaaacgtaaa atcatcggtc
      541 gcgttttcgt tgaagtattc gatgaagaag cgctgaaact ggaagacgtg aagtggctgg
      601 cgcagggcac catctaccct gacgttatcg aatctgctgc ttctgcaacc ggtaaagcac
      661 acgtcatcaa atctcaccac aacgtgggtg ggctgccgaa agagatgaag atgggcctgg
      721 ttgaaccgct gaaagagctg ttcaaagacg aagtgcgtaa gattggtctg gagctgggcc
      781 taccgtatga catgttgtac cgtcacccgt tcccgggacc aggccttgga gttcgtgttc
      841 tgggggaagt gaagaaagag tattgtgacc tgctgcgccg tgctgacgcc atcttcattg
      901 aagaactgcg taaagcggac ctgtacgaca aagtcagcca ggcgttcacc gtcttcctgc
      961 cagtacgttc cgttggcgta atgggcgatg gtcgtaagta tgactgggtt gtctctctgc
     1021 gtgctgtcga aaccatcgac tttatgaccg cacactgggc acatctgccg tacgatttcc
     1081 tcggtcgcgt ttccaaccgc attatcaatg aagtgaatgg tatttcccgc gtggtgtatg
     1141 acatcagcgg caagccacca gcgactattg agtgggaatg atttgactct gcactatgaa
     1201 tgaacaaaac cctctgttac tacagagggt tttttatact cacgaatcat aggattgaag
     1261 ttactaacat cgattaatta aaccagctat ctgatttatt ctcttctgct ttgcccacgc
     1321 ttttcatcag atcgcgaccg ccttcagtca tattcctgtt tgcgtcagct tcagattgca
     1381 ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc gataaattcg ttttctcgct
     1441 taacgcgggc ttcttctttc gccagcgcca gtttttgttt ctgaatctct aagctacgta
     1501 gctcatcttc ataactttga tcgcgttttt tgtccgcagt ggcttcggcg tccagtttat
     1561 cctgacgagc tttcttattc gccgctgccg ttgccgctct tttattagcc gcggcctggg
     1621 cgtttgcgcg acgttgcttc tcttgctgga tttccctgtt gcgctccgcg acccattcgt
     1681 catgctgcct ttgctcttca tttttacctt gctgttccgc ttctgcgaca gccgagagtt
     1741 gatcctgcaa tgatgaggcg atagccggat agcttaagga ggctaagatg gcgcaaagaa
     1801 aaactttctt catgactcct cctgattatt agctcttttc aggacatttg gtatttggct
     1861 gaatacgcgt ttcgttatac gtcgtggtaa taacaacggc taaacctgtc gtaaactggc
     1921 actctttacc cacctgggtg gaggtataca ctttggtgcc ttccttatat gttaaagaaa
     1981 caccttccac taaggtttta tcattcacca tagaacccg
//
LOCUS       DAWDRC010000674         2001 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19548_length_2001_cov_2.150565, whole genome shotgun sequence.
ACCESSION   DAWDRC010000674 DAWDRC010000000
VERSION     DAWDRC010000674.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19548_length_2001_cov_2.150565"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          482 a          616 c          488 g          415 t
ORIGIN      
        1 agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc ggccagagaa
       61 ccgtcagcac cgaaagcgct gccaccaaca gcgtggtttt cggccagtgc agtactttca
      121 gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cgggttactg ctttctggcg
      181 gaattttgcc acggatccag tagcccatca ggatcgggat cactacgatc gccagcagcg
      241 ccgcgcccgc catcgcatag gttttggtga acgccagcgg gccaaacaga cgcccctcct
      301 gcccttccag ggtgaaaatc gggataaacg acagcgtgat aatcagcaga ctgataaaca
      361 gcgccggccc cacttcaaca gatgcatcgg tgatcacctg ccagcgcgtt ttattatcca
      421 gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca ttctcgatca
      481 tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc aacgacataa
      541 tattggcgtt cagtccctga aagtgcatga caataaaagc aatacacaac cccagcggca
      601 acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgca cagactaccg
      661 ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcaatggcg cggtcaatga
      721 gctgactgcg atcgtatgtt gtaactatct ccacgccttc cggcagactg cttttcagcg
      781 tttccagttt gtccttcacg gcggcgatca cttcacgggc gtttttgccg gaacgcagga
      841 tcaccacccc gcccgccact tcgccttcgc cgttcagttc ggcaatgccc cggcgcatct
      901 ccgggccaac ctggaccttc gcaacatcac gcagataaac gggcacgcca ttttcactgg
      961 cttttaaaac gatgtgatta aagtcgtcga gcgtttgcag atagccgctg gcgcgcacca
     1021 tatattccgc ttccgccagt tcgatcgacg aaccgcccgc ttcctggttt gaagcatcca
     1081 gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga ggatcgataa
     1141 ccacctgata ctctttcacc acaccgccca ccgacgccac ttccgcaacg tcagggatgg
     1201 tttttagctc atatttgaga aaccagtcct gcaatgagcg taaatcggcc agatcgtgct
     1261 taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg gcatctggcc
     1321 ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg tactccagca
     1381 cccgcgagcg cgcccagtac ggatcggtgc catcttcgaa aatgacatac acataagagt
     1441 cgccaaactg cgagaaaccg cgcacagtct tcgcgccagg caccgacaac atggtggtgg
     1501 ttagcggata agtcacctga ttttcaacga tttgcggtgc ctgaccggga tagctggttt
     1561 taataatcac ctgcacatcg gaaagatccg gcagtgcatc cactggcgtg ttaatgatag
     1621 tccaggtgcc ccagatactc agaaacaacg cgcccatcag caccagaaaa cggttcgcca
     1681 ccgagcgacg aataatccat tcaatcattg gctattccct caatgcgcat gggtagcact
     1741 ttcagagcgc atccgctcca gtgcgccaga aatattggct tcagaatcaa tcaggaacag
     1801 gccgctggaa accacctttt caccttccgc cagaccagag cgtaacgcgg tgacgccttg
     1861 cgatgcctgg aaaacggcaa cgcgtttcgg tacaaagcgc ccgtcggcat caacggtaat
     1921 cacccgttgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca gcatcggttc
     1981 gctagcggtg ttgagttgca a
//
LOCUS       DAWDRC010000675         1992 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19655_length_1992_cov_1.734125, whole genome shotgun sequence.
ACCESSION   DAWDRC010000675 DAWDRC010000000
VERSION     DAWDRC010000675.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19655_length_1992_cov_1.734125"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    957..966
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          479 a          544 c          464 g          495 t
ORIGIN      
        1 cctttttccg gatgacccat taccggatcg caaaaatatt tcgcctgtgg attcgctgct
       61 ttcacctgac gaacgatacc gaggatatgt tcaccctgct ccgccgatcc cagatagcca
      121 cttaatacgg catcacaggt gtgtaattta tcaatggcgg caatgccttg cacaatttcg
      181 gttaaatggc tgggcggcat cacgcaaccg gtccatttgc cgtattgggt gtgattagaa
      241 aattgaacgg tgttcagcgg ccagacgttc gcgcccaggc ggcgcatcgg aaactctgcc
      301 gcactgttac ccgcatgacc ataaacaacg tgagactgga tagcgagaat atttttcatc
      361 atgttccctg tataaaaacc agggggagtg atttctcact ccccctttcc acttaatgca
      421 ttatttccag caaatcagac agtaattctt tttgccgcga cgcagtaagg taaaacgacc
      481 aaacagacga tcttcttctt taaagaagta ttcaggatcg gactgttttt caccgttaat
      541 ggtgatggca ttggaggcga tagttttacg cgcctgacca cgggaaggtt gcagttcaga
      601 atcgaccagc gcctgcatca ggtctgcgcc cttttccatc tcaaccatcg gtacgccgtc
      661 ctgcgccagc tgttcgaagt ccgcttcact cagcgcactc aaggaaccgc tgaacaggca
      721 ttcggtaata cgttttgccg cctgcaaacc ttcttcacca tgcaccagac gtgtcacctg
      781 ttctgcaagc acatactggg cgcgcggtgc tttaccgctg tttttatctt cttcttccag
      841 ggcgttgatc tcttcaatgc tcatgaaggt gaagaacttc aggaagcggt aaacgtcggc
      901 atccgcagtg ttgatccaga actggtagaa tttgtacggg ctggttttct tcggatnnnn
      961 nnnnnncgcc gccttcagtt ttaccaaatt tggtgccgtc tgctttggtg atcaacggaa
     1021 cggtcaagcc aaacacctga ttctgatgca gacgacgggt caggtcgata ccagaagtga
     1081 tgttacccca ctggtcagaa ccaccgattt gcagcaccac accgtactgt ttgttcagac
     1141 aggcgaagtc ataaccctgc aacaggttgt aggaaaactc agtgaacgaa atcccctgat
     1201 cttcacggtt gagacgctgc ttaaccgctt ctttgttgat catctggtta acggagaagt
     1261 gtttgccaat atcgcgcaga aaggtcagca cattcatatt gccgaaccag tcatagttgt
     1321 tcgccgcgat agcagagttt tctccacagt cgaaatcgag gaacggggca acctgcttgc
     1381 ggattttgtc cacccactcc tgaacagttt cttcagtgtt cagcttacgc tcggcagctt
     1441 tgaagctcgg gtcgccaatc agacccgtcg cgccgcctac cagcgcaacc ggcttgtggc
     1501 ccgcctgctg gaagcgtttc aggcataaca atggaacaag atgccccaaa tgcaagctgt
     1561 cagcggtagg atcgaagccg caatagagcg cgagcgggcc ttgcaccagt ccctctgcct
     1621 acgcctcctc gtccgtcacc tgggctacca gcccccgctc ttgcaattgt ttaatcaagt
     1681 tactgcttgc catcaaaatc tccatgtata taacgactgc acctttgccg gtacacgact
     1741 tttcgccaga tgcgaaagag acatagaata aagtgccaga atcaggagta ccagcgatta
     1801 aagcaagatt tttgcatctt ttcagggtgc aagacgatca atcttccacg catcattttc
     1861 acgctggtac aaaaagcggt catgcaggcg atgctcaccg ccctgccaga actcaatctg
     1921 ttcaaggctg acgcgaaaac cgccccaaaa acttggcaat ggcacttcgc cctgttgaaa
     1981 cttctgcttc ag
//
LOCUS       DAWDRC010000676         1987 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19715_length_1987_cov_2.241718, whole genome shotgun sequence.
ACCESSION   DAWDRC010000676 DAWDRC010000000
VERSION     DAWDRC010000676.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19715_length_1987_cov_2.241718"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    787..796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          498 a          521 c          469 g          489 t
ORIGIN      
        1 ccgaccttgc accacgcttt cggccgattt ataggcttgt ggcgcttctt caaagatgag
       61 ttgtttatcg cgacaaatta cccgactgcc aagttcagtc cgcgagagtt gcgtcgccgt
      121 gtatttcgct gccagacgcc ctttacactc ggtgcgcccc catttacgcc ccgccccatg
      181 cgccagcgag tggagcgttt tttcgttcgc aacaggttta accagccagg agtaatcgcc
      241 gcgtgaaccg ggaataatca ccagaccgtt gtcatccggc gtggcgcctt tacgatgcaa
      301 ccagccctgt tgatcgccga tttggcacgc gctaacaaag ttatgcgcca catccagaac
      361 cggacttccg gtggccttaa cttgttgcat tatgcgcaaa gcgatcagct ggcgattaat
      421 acgcgcaaac gccagcgcat cgtcatgttc cgcaatatac cgtagcgcgt cgtcactgcc
      481 ttcaggcaaa ccatgatgcg aaaacgaggc aatatgccgc tgtaaaatag actgccccaa
      541 tcccctcgag ccgctatgaa tcagcagttg cagatgctgc gcatccagac cagccagtgc
      601 aaacagttcc gcgtcgataa tttgatcaat ctgttgcagt tctgcgaagt ggttaccgcc
      661 accgatggag ccaagcgagt tgcgccacgg atgctgtgca aatgctgacg gcaggttttc
      721 ctccagccag ctttcttcag caacgtcatc cagatctgat aatcgctttt caaacttatc
      781 ggcgttnnnn nnnnnntgcg agcgagaata tctgtttgcc atagcgccat gccgcagccg
      841 atatcattgc cgaccagtgc cgggtaaaaa cgaccaacag agaagaacgc tgcgccaatc
      901 gggtagccgc gtccgggatg taaatctggc atccccacaa cgcgctgcat gttgggtaaa
      961 tttgccgtgg tgtgtaattg ttggatcgct aaactctcga tccacaggtc atcagatgcg
     1021 atggtaaata ccgcatcgga taagggacga atatatttgc ccatgtacca ttccatttat
     1081 gaataaaaat caggaaatgg cagacgaatc cagggcaaga aaaaagaatt aatcctggaa
     1141 aggtatgcaa agagtcaatg ataaaggatt tgtcatgatt tacctccttg cagtttgtgg
     1201 aataagaaaa cgcgcacata ctagacgggc gttattttca ttgcaagctg gatttaatgt
     1261 tgcggtttat atcgcatata aaaattagta aaccacgttc cccggcattc aacgcgctgc
     1321 tgcttaactg tctgaaaacc ataacgttca aaaaagggtt ttgcggttat gctggcgtcc
     1381 accgtaagtt cggattcaga cttaatcaat ggttttaaca aagcgctggc aaccccgcgt
     1441 cgggtgtatt ccggttcaac aaataacata tcgatataat gtccaatgca ggtaataaaa
     1501 ccaaccggtt gtgcattaat aactgcaacc cgcacttgtg atttcgcgag tttctccttc
     1561 cagcgagatt cgtcaatctg cgcccaggcg gcaatttgct gtggagaata gtgctgactc
     1621 gccgtcatcg taacagctcg aaggaaaata gcgcatagtt gctgaaaatc accaggctga
     1681 tagtttctta tttgtatgtt attcataatc ccagcaccag ttgtctttcc atttgcgggt
     1741 ccagcaacgt cacatgcagc cccaccagtc gcacaccgcg cccgccgcgg cgttcatccc
     1801 aggttttacg cgcggtggcg attagatcag atttattcag ccgcggccag acgtgttcct
     1861 gggtggtttg ctgaaaatcg tcgaacttta atttcacccc ctggcgggca atcagcaaat
     1921 caggttttac ctttgccaaa cgacgctcca gttcagggta aagccgctcg atgatcgctt
     1981 cacattc
//
LOCUS       DAWDRC010000677         1983 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19753_length_1983_cov_1.999481, whole genome shotgun sequence.
ACCESSION   DAWDRC010000677 DAWDRC010000000
VERSION     DAWDRC010000677.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19753_length_1983_cov_1.999481"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          577 a          460 c          477 g          469 t
ORIGIN      
        1 gcacccgcaa acagggcata aatgacagta accttccatt taataaactc atcattgtgg
       61 aagaacagcg ttaagccacc gaagacgacc accagaacaa aagtgatcag ggccatcttc
      121 tcaaccttac gaaagcgaac ccagctatat ataagcacaa tcgccgtggc gacgatgagc
      181 gccgcagtag ccgcatagat gtcataaatc ttgtaaaacg cgaaaaagac aaccagcggt
      241 aaaaaatcaa gaaactgctt cattttacga ttccgtaatc aaatgcttaa ggttattggc
      301 gaatcaacat atacaggcgg aacagataga tgagcaatac ggctgaaatc aggttgctca
      361 aggtgttcgc cagtacggca ccaatttccg gggttaatgc ggcaaaagaa gaggcaaaaa
      421 gcagcaacag tgtttttgcc agcaaccagc tcagtactgc gggtgccacc agacgcatat
      481 tcgcccatgt cagccgcata ctgctacgca tcgaggcaaa aacgcccatt ttgtcctgta
      541 ccagcatcac cggtgccaga gccagtaaaa tggccatgat aattcccgga acgaccacca
      601 gcatgatgcc aatctgtact aaaagggtag tcagaaaaat cagaataaat aactttggca
      661 atatcggcgc actggcacca atagctcgca gcgcactgac tctctgaccc gcagacacca
      721 gctggataat taatattacg cccccggcga gaatggcgtt accgattaat cctgaaaaag
      781 tggacgccgc tgaagcctgc agcaaaattt gttgctgttc cggtgacata ttctgaacca
      841 ggtcgaacaa cccactactg ccgctaacgg gtacgccgtc attgagctgc gcaagctgtg
      901 catcactggg tgagaaaaca tgccctaaca ccactgtgat aaacgcacat agcaacgata
      961 ccaacagaat ggtcataaat tgattacgga aaaaatttcc ggtgtcacgg tatacggact
     1021 gcgccgtgat agacatgcac tctccttgag ttttatgcag gtgttaatta gcgggcaatt
     1081 gtaccctggt taagccccgg gtggcagcat caggtcttgt atggaaaagc atatctttgt
     1141 aaagcggagg taattgctgg ccttcaggcg tgggtaaacc gcttcaagtg gcgagcgagt
     1201 taatatttgc gtagcgaaaa tatttaaaaa ttgatttaaa tcacattaac caggattttc
     1261 aatgcaactt ctaaattaat ccagatcaat aaatggtgaa ttaccatatg taatgtgatc
     1321 tgtgtaggat catttgttac cccaatgtgg gtatattcgt cacgttttta taaccataac
     1381 gacggagcgg atatgaaaaa gttaacagtg gcggctttgg cagtagcaac tcttctctct
     1441 ggcagtgcct ttgcgcatga agccggcgaa ttttttatgc gtgcaggttc tgcaaccgta
     1501 cgtccaacag aaggtgctgg tggtacgtta ggaagtttgg gtggattcag cgtgaccaat
     1561 aacactcaac tgggcctgac gtttacttat atggcgaccg acaacattgg tgtggaatta
     1621 ctggcagcaa cgccgttccg ccataaaatc ggcacccggg cgaccggcga tattgcaacc
     1681 gttcatcatc tgccgccaac actgatggcg cagtggtatt ttggtgatgc cagcagcaaa
     1741 ttccgtcctt acgttggtgc aggtattaac tacaccacct tctttgataa tggatttaac
     1801 gatcatggca aagaggcggg gctttccgat ctcagtctga aagactcctg gggagctgcc
     1861 gggcaggtgg gggttgatta tctgattaac cgtgactggt tggttaacat gtcagtctgg
     1921 tacatggata ttgatacgac tgccaattat aaactgggcg gtgcacagca acacgatagc
     1981 gta
//
LOCUS       DAWDRC010000678         1979 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19792_length_1979_cov_2.006757, whole genome shotgun sequence.
ACCESSION   DAWDRC010000678 DAWDRC010000000
VERSION     DAWDRC010000678.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19792_length_1979_cov_2.006757"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    904..913
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1267..1276
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          463 a          525 c          524 g          447 t
ORIGIN      
        1 tgccccaaat ggttatggcg agtgggatgg gtatacatcc ccggacggcg gcgaaccggc
       61 tcaagcccgg tgagtacctc aatggcatca gcgttataag tttgcgtcat ggtttaagtt
      121 agcaattcga gttgatcgtc agagatggtg cagaccaaga aaatcgacga tcgggttgaa
      181 ataatcttcg aagcccgtga atgcgtggtt gccgccttct atgacagtct ggcggcagga
      241 agcgtagtac gccaccgcct ggcggtaatc cagcacttca tctcccgtct gttgcagcag
      301 ccagatcaaa tccggcgctt ccagcgggtc aatctgcatg actttgagat cgtaaatatg
      361 gcgtgactct agcacatatt gctgcccggt gtaggggttc tcgttctgac cgagatagtc
      421 cgtcagcagt tcaaacgggc gcaccgccgg gttgaccacc actgcgggca gcataaaaca
      481 ttgcgacaac caggtggcgt aatatccccc cagtgacgaa ccgacaatac ccagcgaatc
      541 gccgccatgt tccaggacaa tagattccag cagttctgcc gcgtcagaag gatacggggg
      601 caactgcgga atgatcatct caacgtcagg gtgatgttcc gccagccagt tttttaacaa
      661 gctcgctttt gcagagcgcg gcgagctgtt gaaaccgtgt aaataaagaa gcgtagacat
      721 cagtagcctt ctgaagcggt atcaggttgg aagcgtgcgt ccgccaggcg atgcacctcg
      781 gtggtcagcg tgccatcagc atgtaactcg agagtacgcc agccgggcgc gatggtgtcc
      841 agcgtaaaat tagaacagtg cggctgaaac tgcagaaagg tcgacggcgg tgccagccag
      901 cggnnnnnnn nnncgcccgc gttacgcagg ctgtgttgat caagccaact acaacccgca
      961 ggtagcggat gatgatgcag cagcagcaac gtatggcgtt ctggcgcatc ggccagttta
     1021 cgttccagcc actcaagctg aaactcgctc agctcaccgt gcggcacgcc aaacacctgg
     1081 ctatccagca acaggatttg ccattgctca ccaataaaca cgcgcttcgc cggggagata
     1141 cccgcatcct gtaacgcgct gtacatcgcg ggctggaaat cgtggttgcc cggcagccag
     1201 acgcagggcg cacgaaaact tgcgatgcct tcagcgaaat gctgataggg cgcagaggat
     1261 tgatccnnnn nnnnnngcct ggtagctttc ccaggtgttt acccctaaca gggcttcgtg
     1321 cttttgtgca aacaggtgag tgtcggtaat ttgtaaaatc ctgactctgg cctcaccagc
     1381 cagaggaagg gttaacaggc tttccaaatg gtgtccttag gtttcacgac gctaataaac
     1441 cggaatcgcc atcgctccat gtgctaaaca gtatcgcaac cagtccgcta aaaactgatt
     1501 aatttgatgc ttttcgtcgc gttgatgcaa ctttttatta ggataatcat accgcgcttt
     1561 gaagcgaaaa atctgctggc ttgaacacac ttcagccacc atcgcgtcat gatacagacg
     1621 caccgtcatt gacggaaggc tccagtaact gatcgcgggc gcagtctgtt ctattgtcac
     1681 cagggtagtg tatcgggtcg attccacaat cgtcagccga tattgtgcgt ttgccacctg
     1741 atagcttaca gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa
     1801 gttcatctcg cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc
     1861 ccactcattt tttaacgctt gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc
     1921 tgcgttgtcg attttcccct cttctaccca ctggtatgcc tgttcccggc ttaccacat
//
LOCUS       DAWDRC010000679         1964 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_19933_length_1964_cov_1.986380, whole genome shotgun sequence.
ACCESSION   DAWDRC010000679 DAWDRC010000000
VERSION     DAWDRC010000679.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19933_length_1964_cov_1.986380"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    956..965
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          465 a          545 c          483 g          461 t
ORIGIN      
        1 ctgcttcatg atcaacaact gcaccaccct ctccccggat tctggagata tcgccaatac
       61 gctgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
      121 agcgcgcggg gaaatcccga cccactgtga cgtgacgcat ctggcagaat tccttaattg
      181 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca
      241 gattgccgga acgactttgc gtttatggcc cgaactggtg aaataagtcg caaaaaaagc
      301 ccctcagcct gagcgaatga ggggctaaat tgcagagtac agccttgtta tactttgtta
      361 ttcaaaatca gctgcccgtt gctatctagc gggatctgcg tacccggatc gcggtccatg
      421 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
      481 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
      541 agagagccct ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtggcg
      601 acatctttac cgcgaccacc accaaactga tgcccaatca cgccgccagc aacagcgccg
      661 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtaacg
      721 ttgcgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
      781 tgagcgtatt gcgggccacg ttcaaacacg ttcagactgg ccactgccgc tacgcccagc
      841 gcagctgcga caccaatccc gatacccgcc aacattgact tattcacggg aatacctcct
      901 tctttgctgt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagannnnn
      961 nnnnnaagat gaaaagcgcg ggaatggctg gaaatactta gggattcgga ggagtctgaa
     1021 ggaatgcctg atgcgacgct taacgcgtct tatcaggcct accgctcgta ccatttgtcc
     1081 agggcggata aggcgtttac gccgcatccg ccagtacaag ccgattaatg caacttcaaa
     1141 cgtggacgga taacacggtt aatactcccc accagcatca ttaatccggt tttaaagtaa
     1201 ccatgcagcg caatctggtg cattcggtac agcgagatgt atacaaagcg cgcaattcgt
     1261 ccttcaatca tcattgagcc gcgcgtcagg ttacccatca ggctaccgac ggtggaaaag
     1321 ttcgacagcg ataccagcga accgtgatct ttatactgat aatttttcag cggcttaccg
     1381 ttcatctgcg ccagaatgtt gttcattgca caggttgcca tctgatgtgc ggcctgagcg
     1441 cgcggcggaa caaagccccc ttccggacgc gggcatgacg cgcagtcgcc aatagcgtat
     1501 atgtctggat cgcgagtggt ttgcagcgtc ggttccacca ccagctggtt gatacggttg
     1561 gtttcaagac caccgatatc tttcaggaag tctggcgctt tgatcccggc tgcccacacc
     1621 atcagatcag cctcaatata ttcgccatct ttagtgtgca ggccgccttc atcagcactg
     1681 gtgaccatgg tttgcgtcag cacgcgaacg cctagtttcg ttaactcact atgggccgca
     1741 gcagagatac gtggcggcaa tgcaggcaaa atacgttcgc ccgcttctac cagcgttacg
     1801 ttcagggctt cgttggtcag acctttataa ccgtaactgt gcagttgctt gaccgcgttg
     1861 tgcaattcag cggagagttc tacacccgtc gcgccgccgc cgacaatcgc aatgtttact
     1921 ttgccattcg cgcccaggtt ggcggagtat ttcaggaaca gatt
//
LOCUS       DAWDRC010000680         1948 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20099_length_1948_cov_1.781828, whole genome shotgun sequence.
ACCESSION   DAWDRC010000680 DAWDRC010000000
VERSION     DAWDRC010000680.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20099_length_1948_cov_1.781828"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          451 a          471 c          567 g          459 t
ORIGIN      
        1 gtactaacgg ttcgttgaat gccgcagaag cgaccgaaac gctgcgaaat gcgctggcta
       61 ataacggtaa atttaccctg gtttccgccc agcagctgtc gatggctaag caacaattag
      121 gtttgtcgcc gcaggacagt ttaggcaccc gtagtaaagc cataggcatt gcccgcaatg
      181 ttggcgctca ttacgtgctg tactccagcg cctctggcaa cgttaacgct ccgaccctac
      241 aaatgcagct gatgctggtg cagacgggcg aaattatctg gtcaggtaaa ggtgccgttt
      301 cgcagcaata atcccctcac gcgcgacgaa ttgctgtcgc gcttttttcc gcagtttcat
      361 cccgtcacga cgtttaatcg agggcttagt ggcgggagtt ttctcattga acatcagggc
      421 cagcgttttg ttgtgcgtca gccgcacgat cctgatgcgc cgcagtccgc gttcttgcga
      481 cagtatcggg ctttatcaca actacccgca tgcattgcac cgaagccgca tttatatctc
      541 cgtgactgga tggtggttga ctatctgccc ggcgaggtaa aaacgtattt gccggatacc
      601 aacgaactgg caggcttgct gtattatcta catcaacaac cgcgttttgg ctggcgaata
      661 acgctgttgc cgttactgga actgtactgg cagcaaagcg atccggcgcg gcggacagtg
      721 ggttggctgc gaatgttaaa acgtctgcgc aaagcgcggg aaccacggct tttacgctta
      781 agtccattgc atatggatgt ccacgccgga aatttagtgc atagcgcgtc agggttaaaa
      841 ctcatcgact gggagtatgc cggagatggt gatatcgcgc tggaactggc ggcggtgtgg
      901 gtggaaaata ttgaccagca tcggcaattg gtcaatgact atgccactcg cgcgaagatt
      961 tatccggcgc aattatggcg tcaggtcagg cgatggtttc cctggctgct gatgctcaaa
     1021 gcagggtggt ttgagtaccg ctggcgacaa accggcgatc aacaatttat caggctggcc
     1081 gatgacacgt ggcggcagct attaataaaa caataaggag agcagtgtgg gtccagtaat
     1141 gttggatgtc gaaggttacg aactggacgc ggaagagcgt gaaatactgg cgcatccgct
     1201 ggtgggaggg ctgattctct ttacgcgaaa ctatcatgat cctgcccagt tacgtgaact
     1261 ggtgcgccag atccgcgcag catcgcgcaa tcatctggtg gtggcggtag atcaggaagg
     1321 tggacgcgtg cagcgttttc gtgaaggttt tacccgcttg ccagcggcac aatcattcgc
     1381 tgcgctgttg ggaatggaag aaggcggaaa actggcgcaa gaggcaggtt ggttgatggc
     1441 cagcgaaatg attgctatgg atattgatat cagctttgcg ccagtgctgg acgtcgggca
     1501 tatcagcgcg gcgattggcg agcgttctta tcatgccgat ccacaaaaag ccctggcaat
     1561 tgccagccgg tttattgatg gtatgcatga agcgggaatg aaaactaccg ggaaacactt
     1621 cccaggtcac ggtgcagtaa cggcagactc acacaaagaa acaccgtgcg acccacgtcc
     1681 gcaagcggag attcgcgcta aagatatgtc ggtcttcagt tccttaatcc gcgaaaataa
     1741 actcgacgcc attatgcctg cgcatgtgat ctacagtgat gttgatccgc gtccggcgag
     1801 cggttctccc tactggctga aaaccgtttt gcgtcaggaa ttgggttttg acggcgtgat
     1861 tttctctgac gatttatcga tggaaggcgc agcgattatg ggcagttatg ccgagcgtgg
     1921 acaggcatca ctggatgcag gttgcgat
//
LOCUS       DAWDRC010000681         1944 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20143_length_1944_cov_1.871361, whole genome shotgun sequence.
ACCESSION   DAWDRC010000681 DAWDRC010000000
VERSION     DAWDRC010000681.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20143_length_1944_cov_1.871361"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          430 a          562 c          482 g          470 t
ORIGIN      
        1 ccagacaatg tggatgtgaa gtaccgtgtc tggaaggcgg aagagaaaat tgacaacgcg
       61 gttgtccgct aaactgctgt gaagtgctgc aacctgtagg ccgaataagg cggttacgcc
      121 gcatccgaca tctaacgccc gagccggttg cctgatgcga cgctgaagcg tcttatcagc
      181 atacgccaca tccggcaaac catgccggat gtggcacatc attacaacgc aatatccgcc
      241 acttcttttt gaacgggttg cggtttcaac tgcggtttcg gcgtactatc tgctgcctgc
      301 ggcttgtcgt atttcagccc cagcacttcg ctggtgtact gcaactcgcg ttctgttgcc
      361 gctacatcac cgttcagctt gcgtccataa gacggaacga tcgctttcaa cgtagcctgc
      421 cattgcgggc tggaaacgcg atcgccaaat actttttcca gcagattcaa cataatcggc
      481 gcggcggttg acgcccctgg cgatgccccc aggagcgcgg caatggtccc ttgctggtca
      541 ctgacgactt cagtacccag acgcagtacg ccacctttat cggcatcacg cttgataatc
      601 tgcacgcgct gccccgcttg ccacaaacgc cagtcctctt ttttcgcctg cggatagtac
      661 tctttcaacg cttcaaaacg atcctcttca ctcaacatca cctgactcac cagatatttc
      721 accagatcga aattatccag cccaacgtgc atcatcggca tcacgttaga ggtggtggtg
      781 gaactcatca gatcccacaa tgaaccgttt ttgaggaatt tggttgagaa ggtggcaaat
      841 ggcccaaaca gcactacgcg tttaccgtca agaacacggg tatcgatatg cggaaccgac
      901 atcggtggtg cgccaacgga tgctttaccg taaaccttcg ccagatggtg attaaccacg
      961 tccgggtttt ccgaaacaag gaactgtccg cccaccggga aaccagcgta gtctttcgcc
     1021 tccggaatcc ccgattcctg taacagtttc agcgccgcac cgcccgcgcc gataaagaca
     1081 aatttggcgc ggatgttctg tgcagtgccg tttttcagat cggcaacggt gacggtccag
     1141 ctgttatcgt cattacgctt tagggcgcgg acttcgctgc tgagttgcag cgagaagtta
     1201 gatttcttct gtaaggaggc gatcaactgg cgggtgatct cgccatagtt cacatcagtg
     1261 ccaatttctg tgcgtgttgc cgctactttc tgttgcggat cgcgcccttc catcactaac
     1321 ggtgcccact ctttgatctg cgcgtgatct tcagagtaac gcataccgcg aaacagcgag
     1381 ctttgttgca acgcggcgta acgggcgcgc aggaaattaa cgttatcctc gccccagaca
     1441 aagctcatat gcggaacggt attgataaat gaacgcggag tacgcagcac accgcgctcg
     1501 acctggtgcg cccagaactg gcgcgaaatc tgaaatgctt cgttaatggc gactgctttt
     1561 tcaatactga tgctgccatc ggcgttttgc ggggtgtagt tcagttccat cagtgcagaa
     1621 tgcccggttc cggcgttgtt ccagccgttc gaactctcct gcgcgacacc ctccaggcgc
     1681 tccaccatgg tcatcgacca ttcaggctcc agctcgcgta aataggtccc caacgtggcg
     1741 ctcataatgc cgccgccaat caacagtaca tcagtttcct gctcctcgga cgctttcgct
     1801 tttgccgcca tcgaaacggc attaagcccc acggccatcg agaagagcat ggcagtcact
     1861 tttttcatct tgttaatgcc ttacttttag tcgctttatt gcaggtgaga tttgcgcggc
     1921 atcaacggta acacttaagt aaca
//
LOCUS       DAWDRC010000682         1942 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20159_length_1942_cov_1.923688, whole genome shotgun sequence.
ACCESSION   DAWDRC010000682 DAWDRC010000000
VERSION     DAWDRC010000682.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20159_length_1942_cov_1.923688"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1337..1346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          398 a          529 c          524 g          481 t
ORIGIN      
        1 atgagtaatc cgatcctgtc ctggcgtcgc gtgcgggcgc tgtgcgtcaa agagacgcgg
       61 cagatcgttc gcgatccgag tagctggctg attgcagtag tgatcccact gctgctgctg
      121 tttatttttg gctacggcat taacctcgac tccagcaagc tgcgcgtcgg gattttactg
      181 gaacagcgta gcgaagcggc gctggatttc acccacacca tgaccggttc gccctacatc
      241 gacgccacca tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt
      301 cgcggtctgg tggttattcc ggtggatttc gccgaacaga tggagcgcgc caacgccacc
      361 gcaccgattc aggtgatcac cgacggcagt gagccgaata ccgctaactt tgtgcagggg
      421 tatgtcgaag ggatctggca gatctggcaa atgcagcgcg cggaggataa cgggcagact
      481 tttgaaccgc ttattgatgt acaaacccgc tactggttta acccggcggc gataagccag
      541 cactttatta tccccggtgc ggtgaccatt atcatgacgg tcatcggcgc gattctcacc
      601 tcgctggtgg tggcgcgaga atgggaacgc ggcaccatgg aggctttgct ctctacggag
      661 attacccgca cggagctgct gctgtgtaag ctgatccctt attactttct cgggatgctg
      721 gcgatgttgc tgtgtatgct ggtgtcggtg tttattctcg gcgtgccgta tcgcgggtcg
      781 ctgctgattc tgttttttat ctccagcctg tttttactca gtaccctggg gatggggctg
      841 ctgatttcca cgattacccg caaccagttc aacgccgctc aggtggccct gaacgcggct
      901 tttctgccgt cgattatgct ttccggcttt atatttcaga tagacagtat gcccgcggtg
      961 atccgcgcgg tgacgtacat tattcccgca cgttatttcg tcagcactct gcaaagcctg
     1021 ttcctcgccg ggaatattcc ggtggtactg gtggtgaacg tgctgttttt gatcgcttcg
     1081 gcggtgatgt ttatcggcct gacgtggctg aaaaccaaac gtcggctgga ttagggagaa
     1141 gagcatgttt catcgcttat ggacgttaat ccgcaaagag ttgcagtcgc tgctgcgcga
     1201 accgcaaacc cgcgcaattc tgattttacc cgtgctgatt caggtgatcc tgttcccgtt
     1261 cgccgccacg ctggaagtga ccaacgccac catcgccatc tacgatgaag ataacggcga
     1321 gcattcggtg gaacttnnnn nnnnnnaaag cctgcaggag atccgcccga ccatcgacac
     1381 acaaaaggcg ttactactgg tgcgtttccc ggctgacttc tcgcgcaaac tggatacctt
     1441 ccagaccgcg ccgttgcagt tgatcctcga cgggcgtaac tccaacagtg cgcaaattgc
     1501 cgccaactac ctgcaacaga tcgtcaaaaa ttatcagcag gagctgctgg aaggaaaacc
     1561 gaaacccaac aacagcgagc tggtggtacg caactggtat aacccgaatc tcgactacaa
     1621 atggtttgtg gtgccgtcgc tgatcgccat gatcaccact atcggcgtaa tgatcgtcac
     1681 ttcactttct gtcgcccgcg aacgtgaaca aggtacgctc gatcagctac tggtttcgcc
     1741 gctcaccacc tggcagatat tcatcggcaa agccgtaccg gcattaattg tcgccacctt
     1801 ccaggccact attgtgctgg cgattggtat ctgggcgtat caaatcccct tcgccggatc
     1861 gctggcgctg ttctacttta cgatggtgat ttacggttta tcgctggtgg gattcggtct
     1921 gttgatttca tcactctgtt ca
//
LOCUS       DAWDRC010000683         1928 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20335_length_1928_cov_1.990390, whole genome shotgun sequence.
ACCESSION   DAWDRC010000683 DAWDRC010000000
VERSION     DAWDRC010000683.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20335_length_1928_cov_1.990390"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          464 a          541 c          515 g          408 t
ORIGIN      
        1 tttaaggccc cttcctcggg aggggctttc ccgtttcagc gtcccgctga aatcgtcggc
       61 ttacctcctt tcgccatgca agcagtctat cgctaacgcg tagataaaat agtttcatgt
      121 gttattactg gatgcgtgct cgcaaaagtg cccgtcattc agacgattcc cgacagtgtt
      181 tcataattcc tccatttttc tcccttattg gctggctaca ctagtatcat tccgcgaaac
      241 gtttcaggaa gagaaactct taacgatgaa aggcagttat aaatcccgtt gggtaatcgt
      301 aatcgtggtg gttatcgccg ccatcgccgc attctggttc tggcaaggcc gcaatgactc
      361 ccagagtgca accccagggg cgacgaaaca agcgcagcaa tcgccagcgg gtggtcgccg
      421 tagtatgcgt gccggcccat tagctccggt tcaggcggcg accgcagtag aacaggctgt
      481 tccgcgttac ctcaccgggc ttggcaccat taccgccgct aatactgtta cggtgcgcag
      541 ccgcgtggac ggtcaactga tggcgttaca tttccaggaa ggccagcagg tcaaagcagg
      601 cgatttactg gcagaaattg acccaagcca gttcaaagtt gcattagcac aagcccaggg
      661 ccaactggca aaagataaag ccacgcttgc caacgcccgc cgtgacctgg cgcgttatca
      721 acaactggca aaaaccaatc tcgtatctcg tcaggaactg gatgcccaac aggcgctggt
      781 cagtgaaacc gaaggcacca ttaaggctga tgaagcaagc gtcgccagcg cgcaattgca
      841 actcgactgg agtcgcatta ccgcaccagt cgatggtcgc gttggtctca agcaagttga
      901 tgttggtaac caaatctcca gtggcgacac caccgggatt gtggtgatca cccaaacaca
      961 tcctatcgat ctggtcttta ctctgccgga aagcgatatc gccaccgttg tacaggcaca
     1021 aaaagccgga aaaccgctgg tggtagaagc ctgggatcgc accaactcga agaagttaag
     1081 tgaaggcacg ctgttaagcc ttgataacca aatcgatgcc actaccggta cgattaaagt
     1141 gaaagcacgc tttaataatc aggatgatgc gctgtttccc aatcagtttg ttaacgcgcg
     1201 catgttagtc gacaccgaac aaaacgccgt ggtgatcccc accgccgctc tgcaaatggg
     1261 caacgaaggc cattttgtct gggtactgaa tagcgaaaac aaagtcagca aacatctggt
     1321 gacaccaggc attcaggaca gtcagaaagt ggtgatccgc gcaggtattt ctgcgggcga
     1381 ccgcgtagtg acggacggca ttgatcgcct gaccgaaggg gcgaaagtgg aagtggtgga
     1441 agcccagagc gccactactc cggaagagaa agccaccagc cgcgaatacg cgaaaaaagg
     1501 agcacgctcc tgatgcaggt gttacccccg agcagcacag gcggcccgtc gcgcctgttt
     1561 attatgcgtc ctgtcgccac cacgctgctg atggtggcga tcttactcgc cgggattatc
     1621 ggttatcgcg ccctgcccgt ttcggcgctg ccggaagtgg actatccaac cattcaggtg
     1681 gtcacgcttt atccaggtgc gagcccggat gtcatgacct ctgccgttac cgcgcctctc
     1741 gaacgccagt tcgggcagat gtctggcctg aaacagatgt cgtcgcaaag ttccggcggt
     1801 gcgtcagtta tcactttaca gttccagcta acattgccgc tggatgtcgc cgagcaggaa
     1861 gtgcaggccg cgattaacgc tgcgaccaac ctgctaccga gcgatctgcc taacccgccg
     1921 gtttacag
//
LOCUS       DAWDRC010000684         1925 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20361_length_1925_cov_2.033690, whole genome shotgun sequence.
ACCESSION   DAWDRC010000684 DAWDRC010000000
VERSION     DAWDRC010000684.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20361_length_1925_cov_2.033690"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          427 a          555 c          515 g          428 t
ORIGIN      
        1 agcgggcgta agcaagatca ttgttccggc gacagaggcg gaaaattttg cccgcgtgca
       61 ggcgttagcg gaaaaatatc aaccgctgta tgccgcattg ggcttgcatc ctggtatgtt
      121 ggaaaaacat agcgatgtgt ctcttgatca gctacagcag gcgctggaaa ggcatccggc
      181 gaaggtggtg gcggtggggg agatcggtgt ggatctcttt ggcgacgatc cgcaatttga
      241 gaggcagcag tggttactcg acgaacagct gaaactggcg aaacgctacg atctgccggt
      301 gatcctgcat tcacgacgca cgcacgacaa actggcgatg catcttaaac gccacgattt
      361 accgtgcact ggcgtggttc atggtttttc cggcagcctg caacaggccg aacggtttgt
      421 gcaactgggc tacaaaattg gcgtaggcgg gactatcacc tatccacgcg ccagtaaaac
      481 ccgcgatgtc atcgcaaaat taccgctggc atcgttgttg ctggaaaccg acgcaccaga
      541 tatgccgctc catggttttc aggggcagcc taatcgccct gaacaggccg cccgtgtgtt
      601 tgccgtgctt tgcgagttac gaccggaacc ggcggatgag attgcgcaag cgttgcttaa
      661 taacacctat acgttgttta acgtgccgta ggccggataa ggcgttcacg ccgcatccgg
      721 cagttggcgc acaatgcctg atgcgacgct tgacgcgtct tatcatgcct acaggtctgt
      781 gccgaaccgt aggctgcatc cgccatgtca caaatacagc gccggaaata ttaaccggct
      841 caccccgcgc tcccataacg catcagccag ttgttccaca tcctccggcg tggcgctcgg
      901 ccagctttgc gcctcgccat acacgccgtg ggcatgaaac gcgttcaggc gtaccggaac
      961 atcgccaagt cccttgatta acgccgccag ttcttcgatg tgttgcaaat aatccacctg
     1021 gccagggatt accagcaaac gcagttccgc cagcttaccg cgctctgcca gcaaacagat
     1081 gctgcgcttg atctgctgat tatcgcgtcc ggttaattgc tgatgacatt cgctccccca
     1141 cgctttgaga tcgagcattg cgccgtcaca caccgtgagc aatttttccc agccggtttc
     1201 gctcaacatg ccgttactgt ccacgaggca ggtgagatgg cacagttgcg gatcgttttt
     1261 gatggcagta aacagcgcca ccacaaacgg caactgggtc gtggcttcgc cgccgcttac
     1321 cgttatccct tcaataaaca gaaccgcttt gcggacatgg ttaagcacct cgtccacgct
     1381 catggattgc gccattggcg tagcatgttg cggacacatc ttcaggcagg tatcacattg
     1441 ctcgcagaca gccgcgctcc acgccacttt gccgtcgaca atctgcaacg cctgatgcgg
     1501 acactgcggc acgcactctc cacaatcatt acagcgcccc atcgtccacg gattgtgaca
     1561 gtttttgcag cgcagattgc agccctgcaa aaacagagcc agacgactgc ctggcccgtc
     1621 aacgcaggag aaggggataa tcttactgac taaagcgcat ctgctgttca tggcttatca
     1681 cgcgcggctg gcgttccaga atacgagtgt tgcgtgcggc ttcttctccc agccaggtag
     1741 tgttggtgcg tgaaccttcg gcgcgatatt tttctaaatc cgacaaacgc accatataac
     1801 cggtaacgcg aaccagatcg ttaccgctga cattggcggt aaattcacgc attccggctt
     1861 taaaggcacc gaggcaaagc tgtaccagcg cctgcggatt acgtttgatg gtttcgtcga
     1921 gcgtc
//
LOCUS       DAWDRC010000685         1921 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20403_length_1921_cov_1.978564, whole genome shotgun sequence.
ACCESSION   DAWDRC010000685 DAWDRC010000000
VERSION     DAWDRC010000685.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20403_length_1921_cov_1.978564"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1262..1271
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1602..1611
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          400 a          561 c          509 g          431 t
ORIGIN      
        1 tcgaaatagt gggtagcaaa cagcgtcaac gctttgatct tatttgccag attttccgca
       61 cacgcccatg ccagcgacag gccatcgtaa gtggacgttc cgcgcccaat ctcgtccatc
      121 aacaccagac tgtattcggt ggcgttatgc agaatattag cggtttcggt catctccacc
      181 ataaaggttg aacgtccgga agccagatca tccgcggcac ctacgcgggt aaagatgcga
      241 tcgatcggcc caatctcgac cttttgcgcc gggacgtaac tgccgatata cgccatcagc
      301 gcaatcaacg cggtctggcg catataggta cttttacccc ccatgttcgg accggtgatg
      361 atcaacatgc ggcgctgtgg cgacaggttt agcgggttag cgataaacgg ttcgttcagt
      421 acctgttcaa ccactggatg gcgaccttcg gtaatacgaa tgccaggttt atcaataaag
      481 gtcgggcagg tgtagttcag ggtataggcc cgctccgcca ggttaaccag cacatcaagt
      541 tctgccagcg cgctcgcgct ctgttgcaac gcttccagat gcggcagcaa caggtcgaac
      601 agctcttcat aaagctgttt ttccagtgcc agcgctttgc cttttgaggt gagaacttta
      661 tcttcgtact ctttcagctc cggaataatg taacgctcgg cgtttttcag cgtctggcgg
      721 cgcatgtagt tgattggtgc cagatggctt tgcccacggc tgatttgaat gtaatagccg
      781 tgcaccgcgt taaagccaac tttcagcgtg tccaggccgg tacgttcacg ctcgcggact
      841 tccagacgct ccagataatc ggtcgcgccg tcagccagcg cccgccactc atccagctct
      901 tcgttatagc ccggtgcgat aacaccgccg tcgcgtacca gcactggcgg tgtgtcgatg
      961 attgctcgct ccagcagatc gcgcagctcg gcaaactcgc ccatcttctc acgcagtgct
     1021 tgtaccgggg cgctatcgac accttccagt tgcgcgcgca gttctggaag ttgctggaag
     1081 gcatgacgca tacgggccag atcgcgtggg cgagcggtac gcaacgccag acgcgccaga
     1141 atacgttcca ggtcgcccac ctgacgcagt accggctgta actcggcggt gaaatcctgc
     1201 aatgcgccaa tagtttgctg gcgctcaagc aacacgcggg tatcgcgcac tggcatatgc
     1261 annnnnnnnn nacccatcgg cgttacagtg cagtcgagca ctgaagccag cgtattttcc
     1321 gcaccgccgg ccaggttctg agtaatttcc aggttatgac gcgtcgcggc atccataatg
     1381 atgctgtcct gctgacgttc catagtgata gaacgaatat gcggcagggt cgtgcgttgg
     1441 gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgtgg tgcgttctcc
     1501 acaccaaaac cgaccagatc tcgggtgcca aattgcaggt tcaattgctg gcgagcggtg
     1561 tcgatttcaa actcccacag cgggcgacga cgcaggccgc gnnnnnnnnn ntctcggcga
     1621 aatcttccgc atacagtaat tccgctggat tcgtacgttg cagttctgcc gccatcgttt
     1681 cgcggtccgc cggttcgctc aggcgaaaac gaccggaact gatatccagc gttgcgtagc
     1741 cgaaaccttt actgtcctgc cagatagccg ccagcaggtt gtcctgacgt tcctgcaaca
     1801 gcgcttcatc gctgatggtg cccggcgtaa cgatacggac aactttgcgc tcaaccggcc
     1861 ctttgctggt cgccggatcg ccaatctgtt cacaaatagc aaccgattcg ccctggttca
     1921 c
//
LOCUS       DAWDRC010000686         1908 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20543_length_1908_cov_1.766865, whole genome shotgun sequence.
ACCESSION   DAWDRC010000686 DAWDRC010000000
VERSION     DAWDRC010000686.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20543_length_1908_cov_1.766865"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    751..760
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1285..1294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          412 a          530 c          511 g          435 t
ORIGIN      
        1 ggcggcttcg aagatgcgat gttcgcaggc cggatgatct aacagcgcca ccagatagtg
       61 cagcaagttt tccagcgcga tgggcgtggt gtgtgaacgt acccagcgtg gcggcgttaa
      121 cacgggcaag ttgtaaacca tatcgcgcat gacttcgaat gccgctgaac ccgcgccaac
      181 gataattccg gcacgtagtt cggtcaccgg gacacccgct tcacgtaaaa tatccgccgt
      241 ggcctgacga gcacgcagat gatcagattg ttcatgaggc ggcgcctgca atgaactgag
      301 aaagattaat tgctttaccg gtacttcgcg tagcgcatcg cggacgttga gagccacctg
      361 gcgctcctga gcgataaaat caccgccttc gcccatgctg tgcaccagaa aatagacagt
      421 atcaatattc tgcaacaggg ccggaaggtt atccggccag ttgagatcga ctttatggca
      481 actgacatta gccagttgca gttttgcaag tctgtcgacg tgacgtgccg ccgccaggat
      541 ctgatgccct tgctggctga gcgtgcgcac cagatgctga ccaatgtagc cactggcacc
      601 gagaactaaa atgcgttgcg gcacgtctct ctccttaacg cgccaggaat gcgcgccagt
      661 gggcggcaac ttcagccagt tgctcgcgcg agatgtcgag atgcgtcacc aggcggacca
      721 tccgctgggc ggtaagtcgt gagtttctag nnnnnnnnnn aatcagtacg tttctcgctt
      781 tcatgtattc gcctaacgcg gcagcatttt cttccccgac gcgaacaaac agcatattgg
      841 tgtcctgacg catcacatcc gcgcctgctt cacgcagttg ctccgccatc caggcggcgt
      901 tgtcgtggtc ttcctgcaaa cgtgcgacgt tatttttcag cgcatacagc ccggcggcag
      961 ccagaatgcc ggactggcgc atcccgccac cggtcatttt ccgccagcga atggcacgtt
     1021 taatgtaatc acgattaccg acgagtaatg aaccgaccgg cgtcccaaga cctttcgaca
     1081 ggcaaatggt gaacgagtca caatattgtg tgatctcttt cagttcgcag ccgtaagcca
     1141 ccacggcatt aaagatgcgc gcaccgtcaa catgcagcgc cagattgcgc ttgcgggtaa
     1201 attcccatgc ctcttttaag tattcacgcg gcagcacttt gccgttgtgg gtgttttcca
     1261 gactgagtaa tttggtgcgg gcgannnnnn nnnntgaata ctgcccagca ctgctgcgcc
     1321 accggcttca aacagatagt tatgcgcggc ctgaccgacg atatactctt cgccgcgttc
     1381 gcagtgactg agcagagcga ccaggttggc ctgagtgccg gtaggcagaa aaatggcggc
     1441 ttctttaccg gaaagctctg ctgcatagtc ctgcagagca ttaacggtag ggtcgtctcc
     1501 gtaaacgtcg tccccaaccg gggcggccat catcgcttcg agcatggcgc ggctcggtcg
     1561 ggtaacggta tcactgcgta aatcaatcat ggcatgtcct tattatgacg ggaaatgcca
     1621 ccctttttac cttagccagt tcgttttcgc cagttcgatc acttcatcac cgcgcccgct
     1681 gatgattgcg cgcagcatat acaggctgaa acctttggcc tgttcgagtt tgatctgcgg
     1741 tggaatggct aattcttctt tggcgacaac cacatccacc aacaccggac cgtcgatgga
     1801 gaaggcgcgt tgcagagctt catcgatttc agacgctttt tctacacgaa tacccgtaat
     1861 gccgcaggct tcggcaattc gggcaaagtt ggtgtcgtgt agttcggt
//
LOCUS       DAWDRC010000687         1904 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20584_length_1904_cov_2.242834, whole genome shotgun sequence.
ACCESSION   DAWDRC010000687 DAWDRC010000000
VERSION     DAWDRC010000687.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20584_length_1904_cov_2.242834"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          513 a          506 c          467 g          418 t
ORIGIN      
        1 atctggtgac atcatcacca caaacagcat tccgctaacg aacggcttac cacgaaagcg
       61 ataacggtac agcgcaactg ccgtcagtga accgataagc gtagcaaacg tcgccgaaaa
      121 caccgccatt gttagtgaat gctgcgctgc ctgtaacagg ctgtcgttgt tcatcagcag
      181 gctataccat ttggtggtaa aaccctgcca gttgatgcca aagcgcgagc tgttaaagga
      241 gttcacaatc aaaataatga ttgggatata caggtacgcg tagatagcgg tcataaaacc
      301 gccgcgaagc agtcgaccga tcattcgagt tccaccttct tattcagcag acgagaagcg
      361 cgccagtaaa ccagcagcat caggcccatt acgatagtca gcgtaatgct ggtggctgca
      421 ccaaacggcc agtcacgaat attgaggaac tgtaccttga tgacgttacc gatcagcagg
      481 tttttcgcac cgcccatcag gtcggacaca tagaacaggc ccatcgctgg cagcatcacc
      541 agcagacatc cggcaataat tcccggcatc gtcagtggga taatgatgcg gataaaggtc
      601 tgcaacttgc tggcaccgag atcgcgcgcc gcctcaagca acggcttatc cagtttttcg
      661 atactggagt acagcggcat caccataaac ggcagcagaa tgtaaaccag accgataatc
      721 accgcactag gcgtgaacat gatgcgaatt ggtgtatcga taacgcccag ccagagcaaa
      781 aactcgttga gatagccttt ggtgctgagg aaaattttca gcccgtaaat acgaatcaat
      841 gagttggtcc agaacggaac aatcagcaga aacagcagca gcggacgcac cttgtgtggc
      901 agcttcgcca gaaaccaggc aaaaggatag cccagcacca ggcaggcgag ggtggcgatc
      961 agcgccatat tcagcgagtg caatagcact tcaaaataga gcggatcgag cagacgcgtg
     1021 tagttatcca gcgtaaagac cattttgacg aaactggcgt cgtcgcgggt caaaaagctg
     1081 gtgccaatga tcatcaggtt gggcagaaag acaaacaaca caagccaacc gacaatagtg
     1141 acaatcacta cattctggaa cttacttgtg ttcttcatca gccagtacga cctcccagct
     1201 ttctacccaa ttaatggcca ttttttggtc gagagagtgg tcaaagtcag gatcgtcttc
     1261 attgaagaat tcgctgacca tcaccatctt gccattttcc agctcaacaa ccgactccag
     1321 cgtcatgcct ttgtagttac gctcgcgaac gtaaccaatc agcccttcgg cgtggttgtc
     1381 gtcgttaatc tcttcaacac gtaagtcttc cgggcgcagc agaacatgca gtttttgccc
     1441 cggttcaacg gcgaagttaa cgtagatatt acattcgcgg ccttcaacgt tggcgcgtac
     1501 gcgctgctcg tcgagacgtt cgatgacagt ggcgttaaac atattgattt cgccaataaa
     1561 cccggcaaca aacaggtttt tcggctcttc gtagatttca cgcggcgtgc cgtcttgctc
     1621 aatgcgacca tcgcgcatca ccacgatcct gtccgacatg gtaagtgctt cttcctggtc
     1681 gtgagttacg aacacgaatg taatgccaag cttacgctgt aacgctttca gctcgttctg
     1741 catttgcttg cgcagtttgt aatccagcgc tgagagcgac tcatccagca acaacagacg
     1801 aggcttgtta accaccgcgc gagcaatggc gacgcgttgc tgttgaccac cagagagctg
     1861 atgcggtttg cgttgagcga aggtttccag ctgcaccatc cgca
//
LOCUS       DAWDRC010000688         1898 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20647_length_1898_cov_2.394466, whole genome shotgun sequence.
ACCESSION   DAWDRC010000688 DAWDRC010000000
VERSION     DAWDRC010000688.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20647_length_1898_cov_2.394466"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    237..246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          447 a          454 c          451 g          536 t
ORIGIN      
        1 catcatacaa ttatataacc atttcccggt atcgcttcgc tttagcgaga actggtcttt
       61 tattcgcact caggagtaca tgtatgagga tttgcagcga ccaaccttgt attgttttat
      121 tgactgaaaa agatgtctgg ataagggtga atgggaaaga atctattagc cttaaagcta
      181 accacatggc gttattaaat tgtgaaaata atattatcga cgtctcctct cttaatnnnn
      241 nnnnnncttt ggttgctcat attagtcacg acatcatcaa agattacctc cggtttctga
      301 ataaagatct ctcgcaaata ccagtatggc aacgtagcgc tacgcccatc ctcaccctgc
      361 catgcctgac gccagacgtc tttcgcgttg ccgcgcaaca tagcatgatg cccgctgaaa
      421 ctgagtcaga aaaggaacga acacgtgcat tattattcac tgtgctatcc cgttttctcg
      481 acagtaaaaa attcctttca ctaatgatgt atatgttacg taattgtgta agtgacagcg
      541 tttatcaaat tattgaaagc gatatccaca aagactggaa tcttagtatg gtagccagtt
      601 gtttatgtct tagcccaagt ctgttaaaga aaaagctgaa aagcgaaaac accagttata
      661 gccaaataat caccacctgc cgcatgcgct atgccgtaaa tgaattaatg atggacggta
      721 aaaatatctc tcaggtatca cagtcctgcg gctacaacag tacgtcgtac tttatttctg
      781 tctttaaaga cttctacggt atgacgccgc tgcattatgt tagtcagcac agagaacgca
      841 ctgtcgcctg atttttaacc ttaacgaaga gctatattaa taacggcatc agcgataacc
      901 cggccgataa taattcaact atcgaatgca ggcgtatgat atgacgtaat ttattgtcac
      961 gaagctcgcc ttcgcaggag tttaattatg tcttcggatg ctgatgctca caaagtgggc
     1021 ttaatccccg tcaccctgat ggtgtcgggg aatattatgg ggtcaggtgt ttttctgtta
     1081 cctgcaaacc tggcctccac tggcgggatt gccatttatg gatggttggt gacgattatc
     1141 ggtgcgctgg ggctctcgat ggtatacgcc aaaatgtcgt tcctcgaccc aagtcctggt
     1201 ggttcttacg cttacgcccg ccgctgcttt ggcccgtttc tcggttatca aaccaacgtc
     1261 ctctactggc tggcctgctg gatcggcaat atcgccatgg tggtcattgg cgtaggatat
     1321 ttaagttact tcttcccgat tctgaaagat ccattggtat taaccatcac ctgcgtcgtg
     1381 gtgctgtgga tcttcgtcct gctgaacatt gtcggtccga aaatgatcac ccgcgtacag
     1441 gccgttgcta ccgtgctggc gctgatcccc atcgtcggga ttgccgtatt tggctggttc
     1501 tggttccgtg gtgaaaccta tatggcggca tggaacgtca gcggcctggg caccttcggt
     1561 gcgattcaaa gtacccttaa cgttacgctg tggtcattca tcggtgtgga aagtgcttcc
     1621 gttgccgcag gcgtggtgaa aaacccgaaa cgcaacgtcc ctatcgccac cattggcggg
     1681 gtattgattg ctgccgtttg ctatgtactt tctaccaccg cgattatggg gatgatccct
     1741 aatgccgcac tgcgcgtttc tgcttcgcca ttcggtgatg ccgcacggat ggcgttgggt
     1801 gacaccgccg gggccattgt ttccttctgc gcagctgcgg gttgcttagg ttcactgggc
     1861 ggctggacgt tgctggcagg tcaaacggcg aaagccgc
//
LOCUS       DAWDRC010000689         1885 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20795_length_1885_cov_2.601093, whole genome shotgun sequence.
ACCESSION   DAWDRC010000689 DAWDRC010000000
VERSION     DAWDRC010000689.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20795_length_1885_cov_2.601093"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          483 a          482 c          443 g          477 t
ORIGIN      
        1 ctcttcaaat tcacccatgg taaacgcggt gtgagaatcc gagcctaatg ccacccaacc
       61 tccagcgtca cgtaccgctg cggctaccgc acgacaattg tcttcacttc ccttacgtga
      121 gtgtaaaaaa gaggaattat tgatttccag cgccacctga tgtttcgcgg ctgcttcagc
      181 aacggcttta acatctattt catatttcgg atttccggga tggcttatta tatgcacatt
      241 gccgctggcg atagtggcga tcatcgcttg cgtattggtc gctttgtcat gcggcgcaaa
      301 caccggctca tgaaaaccgg caataatcag atccagcgaa tcaaacattt tgccactgca
      361 gtcaatttca ccgtcaacat ttttaatgtt agcttcgatg ccgcgtagga tcccaacccc
      421 atcaaccact cgcggccaga tacgcatgtt aatgaagtgc cagtgatgcg gcgcatcttc
      481 catatccggg ccatgatcgg tgatcgcaaa aagtttaatg cccttttgtt tggcctgggc
      541 aatgtaatca ctcaatgtgc tatatgcatg cgtgctggca acggtatgca tatgaaggtc
      601 gacgggatac ataactctct cctgtattca attcccgcca aggatagcag gaaaccagat
      661 gctttattag tagccgcgtg cgcggtcgac ttgcccgcag agcctctccc ctttttcgag
      721 ctgggcaatg gtgcgagaaa tgtactccac agcttcagct ggacgggtaa tcgcggcgac
      781 atgtggtgtt atcgtcacgc gtggatgttg ccagagcaga ctttccggcg gtaagggttc
      841 acgattaaaa acatccaaca ttgcgccttt aactttcccg ctatccagcg ccgcgagcag
      901 gtcatcttcc acgacatgaa caccacgcgc caggttgagg agatacgcgc catccggtaa
      961 tttttcgatt aattgttgat taataatgcc gacggtttca ggggtattcg gtaacagatt
     1021 aatcaatacc cggcattggc tcagaaatgc agacagttct tctcgtccgg caaagctttg
     1081 cacgccaggc cacgatttac gggttcgact ccagcaacgc agcgggaagc gccaggtttg
     1141 cagactctgt gcaactttac tgcccagtac gcctgcgccc aaaatgccga tggtaaaatc
     1201 ttcccgatga tattcaggaa gcggttgcca atgcgaacta ttttgctgga tgcggtaatc
     1261 gtcaaaacgt cgaaaccaat gcagcacctg actgacagca tattcctgca tttgctcgcc
     1321 cataccggta tcttccaggc gaaaaagtgg aacagaaggc ttcagcattt cagggtgtgc
     1381 ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt gcgaacaccg ctttaagatc
     1441 gcgccctgcc agcatttcaa ctggaggatg ccagactaaa gcataatcag cagagtcatt
     1501 atctccgctt ttccatgctc tgactcttgc ctgaggaata gctttgcgca gtgcctcaat
     1561 ccaccattgg gtatcgaacg ttgggtgata aaagatgatg tccatactga ctcccgaaaa
     1621 gcgttgcgca aaatttattc gcacttatcg ttatgatcta caaaggccac cagcataaca
     1681 aatccgtggt cggtggcaaa aaaagcagat ttcgcttatt aaaactgcat attgattgaa
     1741 gtttgaataa acgcgcgatt ttttaaaaaa gtttgttgac ctcaggtcat gatttcccta
     1801 aattagcgcc cgttccagca agacaggaac aacaatttgg tgaggtgtcc gagtggctga
     1861 aggagcacgc ctggaaagtg tgtat
//
LOCUS       DAWDRC010000690         1877 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20906_length_1877_cov_2.445115, whole genome shotgun sequence.
ACCESSION   DAWDRC010000690 DAWDRC010000000
VERSION     DAWDRC010000690.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20906_length_1877_cov_2.445115"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1585..1594
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          496 a          419 c          478 g          474 t
ORIGIN      
        1 atgctgtatg caaaccagcc agtcacggtg gcaaacagtg cgttcagcgc ggcgatcagg
       61 gcgataaagc cgatcagcat cgcggcaacg ataatggcaa ccttgaaacc tgccagaatg
      121 tattcaccca gcatttcgaa gaagctctga ccttcgtgca ggttggacat ctgaatattt
      181 tcttcactgg catcaacacg gtaagggttg atcagcgaca gcacgataaa ggtgctgaac
      241 atgttcagta ccagcgccgc aacgacgtat ttcggctcca gcatggtcat gtatgcacca
      301 acgatggaca tcgacacggt ggacatcgcc gttgctgcca tggtgtacat acgattgcgg
      361 gagattttgc cgaggatatc tttataggca ataaagtttt cagactgacc cagaatcagg
      421 gagctgacgg cgttaaagga ttccagtttg cccatgccgt tgactttgga gagcaggaaa
      481 ccaattgcgc ggataatcac cggcaatacg cgaatatgct ggagaatacc gatcagcgca
      541 gagataaaga cgattgggca cagcactttc aggaagaaga atgccaggcc ttgatcattc
      601 atgctaccaa agacgaagtt agtcccttcg ttggcaaaac cgagcagttt ttcgaacatt
      661 tcggagaagc ctttcacgaa gcccagacca acgtcggagt tcaggaagaa ccacgccagt
      721 aacacttcga taacaagcag ttgaataaca taacggatac gaattttttt gcggtcgctg
      781 cttacaagca gtgcgagaat cgcaacaacg gcaagtgcca gtacaaaatg aaggacgcgg
      841 tccatatttg ctccaaatat gaggcaggtt aaatttccgt gcacattcta tgtaacatgg
      901 ttaaggaaaa cgagatctga cacacactat aatgacgtcc tgtgacaagc ttcaaaaata
      961 gtgatctgtc atacatttct gctatgttgt gtatggaagc ggaaggttat gtaaatgtgc
     1021 taatattaaa cataaccata acattcgagt catctatctt ttcgtagtca taatcctggt
     1081 ctatcagaga aatcatcaca acccatttaa atgaatttga taatcattct catttaacgt
     1141 agcataaaac atagcaaagg ctatgtttta gaggcacaag atgacgaact atcgcgttga
     1201 gagtagcagc ggacgggcgg cgcgcaagat gaggctcgca ttaatgggac ctgcgttcat
     1261 tgcggcgatt ggttatatcg atcccggtaa ctttgcgacc aatattcagg cgggtgccag
     1321 cttcggctat cagttattgt gggttgtcgt ttgggctaac ctgatggcga tgctgattca
     1381 aatcctctcg gccaaactgg ggattgccac cggtaaaaat ctggcggagc agattcgcga
     1441 tcactacccg cgtcccgtcg tgtggttcta ttgggttcag gcagaaatta ttgcgatggc
     1501 aaccgacctg gcggaattta ttggtgcggc gatcggtttt aaactcattc ttggtgtttc
     1561 gttgttgcag ggcgcggtgc tgacnnnnnn nnnngatcgc gactttcctt attttaatgc
     1621 tgcaacgtcg cgggcaaaaa ccgctggaga aagtgattgg cgggttactg ttgtttgttg
     1681 ccgcggctta cattgtcgag ttgattttct cccagcctaa cctggcgcag ctgggtaaag
     1741 gaatggtgat cccgagttta cctacttcga aggcggtctt cctggcagcg ggcgtgttag
     1801 gggcgacgat tatgccgcat gtgatttatt tgcactcttc gctcactcag catttacatg
     1861 gcggttcgcg tcaacaa
//
LOCUS       DAWDRC010000691         1877 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_20907_length_1877_cov_2.385291, whole genome shotgun sequence.
ACCESSION   DAWDRC010000691 DAWDRC010000000
VERSION     DAWDRC010000691.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20907_length_1877_cov_2.385291"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    304..313
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1144..1153
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          439 a          435 c          530 g          453 t
ORIGIN      
        1 caaacgtcag gagacgctct accgtatcag gtgcatcagt tcgccatcgc cccggcgagc
       61 cgtgaactgc tccatcaacg aattgagcag cgttttcatc agatgttggc ttcaggtttt
      121 gaagcagaag tccgggcgct ttttgcccga ggagatttgc atacggattt gccttccatt
      181 cgttgcgtgg gttatcgcca gatgtggtct taccttgaag gcgaaatctc atacgatgaa
      241 atggtttatc gaggtgtttg cgccacgaga cagttggcga agcggcagat aacctggctg
      301 cgtnnnnnnn nnngctgcgt ggttgggaag gggttcactg gcttgacagt gaaaaaccag
      361 aacaggcgcg tgacgaagta ttacaggttg ttggtgctat cgcaggctga atgtgtacaa
      421 ttgagacgta tcgtgcgcaa ttttttcaga atcgaaaggt tcaaagtaca aataagcata
      481 taaggaaaag agagaatggc taaggggcaa tctttacaag atccgttcct gaacgcactg
      541 cgtcgggaac gtgttccagt ttctatttat ttggtgaatg gtattaagct gcaaggacaa
      601 atcgagtctt ttgatcagtt cgtgatcctg ttgaaaaaca cggtcagcca gatggtttac
      661 aagcacgcga tttctactgt tgtcccgtct cgcccggttt ctcatcacag taacaacgcc
      721 ggtggcggta ccagcagtaa ctatcatcat ggtagcagcg cgcagaatac ttccgcgcaa
      781 caggacagcg aagaaaccga ataaggtttc gggctgtttt tttacacggg gagccagcga
      841 tcctgcgttc cccgctgatc tatttagagg gttatacgct tgtttgaccg ttatgatgct
      901 ggtgagcagg cggtactggt acacatctat tttacgcaag acaaagatat ggaagacctc
      961 caggagtttg aatctctggt ctcttccgcc ggtgtcgaag cattgcaggt gattaccggt
     1021 agccgtaaag cgccgcaccc aaagtatttt gtaggtgaag gtaaagcagt tgaaattgcg
     1081 gaagccgtaa aagcgacagg agcttcggtc gttctttttg accatgccct gagcccggcg
     1141 cagnnnnnnn nnncggcgca ggagcgtaac ctggagcgtt tgtgcgagtg tcgtgtcatc
     1201 gaccgcaccg gccttatttt agatattttc gcccaacgtg cgcgtaccca tgagggtaag
     1261 ttgcaggttg agttggcgca gttgcgccat ctggctacgc gcctggtgcg tggctggacc
     1321 caccttgaaa gacagaaagg cgggattggt ttgcgtggtc cgggtgaaac ccagctcgaa
     1381 accgaccgtc gtttgttgcg taatcgcatc gtgcagatac agtcgcgcct ggaaagagtt
     1441 gaaaagcagc gtgagcaggg gcggcaatcg cgtatcaaag ccgacgttcc tactgtttcg
     1501 ctggtgggat ataccaacgc cggtaaatct acccttttca atcgcatcac cgaagcgcgg
     1561 gtctacgcgg cagaccagtt gtttgccacc ctcgacccga cgttgcggcg tattgacgtt
     1621 gcggatgtcg gtgaaaccgt gttggcagat accgtagggt ttattcgcca cctgccgcac
     1681 gatctggtgg cggcatttaa agccacgtta caagagacgc ggcaagccac attgctgctg
     1741 cacgtcattg acgcggcgga tgtgcgtgta caagaaaaca tcgaagcggt gaatacggtt
     1801 cttgaagaga ttgatgctca cgagatccca accctgctgg tgatgaacaa gatcgatatg
     1861 ctggacgatt tcgaacc
//
LOCUS       DAWDRC010000692         1864 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21074_length_1864_cov_2.372582, whole genome shotgun sequence.
ACCESSION   DAWDRC010000692 DAWDRC010000000
VERSION     DAWDRC010000692.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21074_length_1864_cov_2.372582"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          466 a          428 c          504 g          466 t
ORIGIN      
        1 gtactggcta aatgttatgg cggcgatatc agtcgtaaga aaaagctgct gcagaagcag
       61 aaagaaggta agaaacgcat gaagcagatc ggtaacgtcg agctgccgca ggaagcgttc
      121 ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg catggcgaat
      181 atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg gtgtgtggat
      241 aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc ggctgccggt
      301 gactcactgg ataaagcaac gctgaaaaag gttgcgccga agcctggctg gctggaaacc
      361 ggtgcttctg tttttccggt actggctatc gtattgattg tgcgttcgtt tatttatgaa
      421 ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga ttttattctg
      481 gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct gatcgaaacc
      541 ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc aaagcttgat
      601 tacatcaagc gcgcggtggg tttgccgggc gataaagtca cttatgatcc ggtctcaaaa
      661 gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc gctaccggtc
      721 acttactcca acgtggaacc gagcgatttc gttcagacct tctcacgccg taatggtggg
      781 gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa tggaattcgt
      841 ctttccgagc gtaaagagac attgggtgat gtgacgcacc gcattctgac agtgccgatt
      901 gcacaggacc aggtggggat gtattaccag cagccagggc aacaactggc aacctggatt
      961 gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc ggacagccgt
     1021 tactggggtt ttgtgcctga agcgaatctg gtcggtcggg caacggctat ctggatgagc
     1081 ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat tggcggtatc
     1141 cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga attatttatg
     1201 agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata ttcccccgaa
     1261 gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt ccccatatac
     1321 cggtaaactg aaactgcagt gaagcagtta gccgagccat gtatatcagg tctgtttcgt
     1381 gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg taattaatcg
     1441 gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc aggcattaac
     1501 tcatcgtagt gccagcagta aacataacga gcgtctggaa tttttaggtg actctattct
     1561 gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg aaggcgatat
     1621 gagccggatg cgcgccacgc tggtccgtgg taatacgctg gctgaactgg cgcgcgaatt
     1681 tgagttaggt gagtgcttac gtttagggcc aggtgaactt aaaagcggtg gatttcgtcg
     1741 tgagtcaatt ctcgctgaca ccgtcgaggc attaattggt ggcgtattcc tcgacagtga
     1801 tattcaaacc gtcgagaaat taatcctcaa ctggtatcaa actcgtctgg acgaaattag
     1861 ccca
//
LOCUS       DAWDRC010000693         1859 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21130_length_1859_cov_2.455654, whole genome shotgun sequence.
ACCESSION   DAWDRC010000693 DAWDRC010000000
VERSION     DAWDRC010000693.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21130_length_1859_cov_2.455654"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          504 a          477 c          384 g          494 t
ORIGIN      
        1 gactttatta tcgtaataaa aatcgcccaa acactcctgt tcgggcgatc aaattaatta
       61 ttgtttatcc tgcatatacg gcgtgtaaac cccgttcaga ctgtgcagga aagcgacgat
      121 atcatccaca tcctcctgcg gcagctcttt gcctacctga tagcgcagca tcagtttcac
      181 cgccccgtcc agcgtcggca cgtcaccgcg atggaagtac ggtgccgtta aagcaacgtt
      241 acgtaaaccg ggtactttct gacgcaattt atcacgcact tctttagtaa cattcatacg
      301 accaatatcc gccgccgtaa tttcaccaaa gttaaagtct tttttcagcc ccaacggttc
      361 aaaggaacgt ccgccgagaa taataccacc atgacaagtt gcacatttat tatctttaaa
      421 taattgatag ccttttttct gttgcgccgt cagagcattc tcatctccac gcaaccattt
      481 atcaaatggg gaatccggcg taattaatgt tttctcaaat tcagcaatgg catcagtaat
      541 attttcgcca ctgaaacctt gcggatagac ttcgaggaac tgcgctttaa gctgcggatc
      601 tttttccagc ttagcaataa tttcgtccca ggctttcgac gccatttcaa tcgggttcaa
      661 cggcggtcca ccagcctgat cctgcaatgt tgccgcacga ccatcccaga actgctcaac
      721 gttaaatact gagttaaata ccgtcggcgc gttaatcggc ccaactgcgc caccaacacc
      781 aatcgatgtt tttctgccat cgacgccccc cgcattcaac gcatggcaat gagcgcatga
      841 aatggtgcta tcagccgata aacggggatc gtgatacagc gcaaaaccca acgccacttt
      901 ttgcgcatcg gtaggcagtt tttgcgggat gggctgcacc ggttcattgc gatgctccgg
      961 agcagtatca ttgctggcgt aatattccgc gcgctgtttt gcaatccagg ccagtatttc
     1021 cgcccgctct tcatcactca ccttacccgc ccagtgaagc gcggtataac gcgttggcgg
     1081 catagtttca tactgcatca cccattcaat cttattcaga tcgctttgcg aaacgggttt
     1141 attagccagc agagccgcac gcacggcctc gaggttaaaa gatttatatc caagctttat
     1201 gtcgtaatcc atcaactgtt tcgcgccggg aatataataa taggcaggta attctgccga
     1261 aggcgtatgg caataatcgc atcctttttc gcggagaaag cctaaaacct tattattttc
     1321 actgacagca gatgcctgaa catcggcctg tttactgcgt ttattatcgt ggtaccacac
     1381 ataaccagat aaccctaaat agcaaatcgc gacgccagcc aggccgatcg cggtaatacg
     1441 tgagaccatt ttcattattc ttccctcacg gtggtgagtt attgtgaccc agaaaatttt
     1501 ccagaatgct gcaaacgtct ctgcgagaca aagccatcat aaaaaacagg gagatatgct
     1561 ttttgatagg aatcaatcaa ttacatgaat ataaactatc aataagatag ccagtgccta
     1621 ttatccagct caggaatggc atatcatgat tgcggcgaga gcaggattga atgttctgaa
     1681 aatgaaaaac aacacgaaat ccgtattttt ataaattcca ttgatattag tgcgttatat
     1741 tttttggcag gttaataaac gtgatccacc gcacgctttg tcgcccacca ggcggagcga
     1801 atgactaccc ttaaagaaaa gcccgataat tagcgacgaa tttcggaggt cggatcctt
//
LOCUS       DAWDRC010000694         1855 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21178_length_1855_cov_2.418333, whole genome shotgun sequence.
ACCESSION   DAWDRC010000694 DAWDRC010000000
VERSION     DAWDRC010000694.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21178_length_1855_cov_2.418333"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    311..320
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          527 a          483 c          439 g          396 t
ORIGIN      
        1 tcaaaatggt ttaaatgact tattaatctc gatttactag tgtgataaac tgaaagttaa
       61 tttattcata accaattgtt tttacgacag ttaatcgttg ctctcctgta tcatattcgt
      121 cggcgtgcta aacagaaaac gggtagcaaa ggccaatacc acgatgatcg cgacacaagc
      181 caacgtccac tctcccatct gagaaaagaa tcgctggtaa gcggcaattg ccataccgct
      241 aatttgcgac tctgtcgtct gctgtgccac gacgcctgcc agccagttgg cgaccgcgcc
      301 tgttgccagc nnnnnnnnnn ggttaatacg ccagacattt tcagacgcgt gatttgcgca
      361 atcgccaccg ggtcaataaa gagttcagca aagcccatta gcgccagccc ggatatcatc
      421 acgcccattg acgcttgacc gccagccgct gcatgtcggg catcaaatgc cagcaacata
      481 aagccacaag ccatcagcag taagccaaag gcaaacttca gccagacgcg caatgctgaa
      541 ttgccgcggc tttctggcga cgccagccag gccagaacaa ccccagcgag catcaccgca
      601 atagcattca ccgactggaa tagtgctgta ggtacttcaa tattgaatgc ctgacgattc
      661 acaaagcgat cgataaacaa gctgatggta ctgccgccct gttgtgccag tacccagaac
      721 aacgtcccga caaacatcaa caatacaatt tgccaaagag cacggcggtg ttcggggaat
      781 ttgatcatca tgcgggcaat gatttgtgcg gcaatgaggc aaacgatcgc cagcaaatat
      841 ccggaccagt cgttctccag cagcagggta aaaaatactg gggctaaaca gagcatcacc
      901 actaaccagc tccatactgg taaggcaaat ttcacgctag tgagcgcttt cttatccata
      961 ctacgcgtgg actggaaatg acgatgaccg cttaagaaaa tcaacaaacc gataaacatg
     1021 ccgccacccg caagggcaaa gccaacatgc catccatacc actgagcagc caggccgcag
     1081 gcgatggggg ctgcgataga accgatattg cccgcagcat acagcagcga aaaaccgcca
     1141 tcacgtcgat gatcgttctc gtcgtagagc tcgccaagca aacagctgat gtttgatttg
     1201 aataaaccgt agccacaaat aatgattgcc agcgccagat acaggctaaa ggttgaattt
     1261 gtatcaatgc ccagcaccac atggccaagg gtcattaaca gcgcgccggc aatcactgca
     1321 gtgcggttgc cgagcaggcg gtcggcaagc cagccgccga gaataggggt aacgtaaacc
     1381 agagaagcat atgcgctgaa caggctgatg gcatggttat catcaaaacc aagctgatgg
     1441 gtgagataga gaatgagtaa ggcacgcatg ccgtaaaaac tgaagtactc ccagatttgg
     1501 atcgccacga tatagtatat cgcgcgcggc tgtgagggtg ttttcatgtg ttctccttat
     1561 gagcaaaaaa gggaagtggc aagccacttc ccttgtacga gctaattatt ttttgctttc
     1621 ttctttcaat actttaacgg tatagcggcc atcagcctga cggtatgcac cgtgaatatc
     1681 ggtttcaaag cccggatagt gagcgccgat ttcacacagc atctgcagga actccagaac
     1741 cggacggctt tcttcggtga tcatttcacc cggcattacc agaggaactc ccggcggata
     1801 cggaaggatc atattggcgt taatacgacc taccatttcg tcgaggtaaa cttct
//
LOCUS       DAWDRC010000695         1847 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21278_length_1847_cov_2.055246, whole genome shotgun sequence.
ACCESSION   DAWDRC010000695 DAWDRC010000000
VERSION     DAWDRC010000695.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21278_length_1847_cov_2.055246"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          411 a          466 c          494 g          476 t
ORIGIN      
        1 ccatagcaca atgattcagg agaaagcatg gtacaaattg tcaggagcgc aagttgcttc
       61 aggccgcgtg tgaggcatct tcctcttcgg attcagcacc gacgacctgt ggggacggta
      121 atttgccggt tttcagaatg gtgctcagca catctttttg ttctgccagc catagcgaaa
      181 gggcttcacg ttgctcgtct tccatttgca ctggagaatt tgtcagccag gtgtcaagca
      241 gatcggccgt atcaagcatc ttgtcataag catcggcttc ctttttgctg gtaaacgaca
      301 ttttctcttc gccctcacga atgactacgt atttaacttc aaccgccatt tgcagcctct
      361 cataataact gtgattttat acagtatatt tcttttcagt tgggaaatca acatcagcaa
      421 taaagacaca cgcaaacgtt ttcgtttata ctgcgcgcgg aattaatcag gggatattcg
      481 ttatgacgtt attaggcact gcgctgcgtc cggcagcaac tcgcgtgatg ttattaggct
      541 ccggtgaact gggtaaagaa gtggcaatcg agtgtcagcg tctcggcgta gaggtgattg
      601 ccgtcgatcg ctatgccgac gcaccagcca tgcatgtcgc gcatcgctcc catgtcatta
      661 atatgcttga tggtgatgca ttacgccgtg tggttgaact ggaaaaacca cattatatcg
      721 tgccggagat cgaagctatt gccaccgata tgctgatcca acttgaagag gaaggactga
      781 atgttgtccc ctgcgctcgc gcaacgaaat taacgatgaa tcgcgagggt atccgtcgcc
      841 tagcggcaga agagctgcag ctgcccactt ccacttatcg ttttgccgat agcgaaaacc
      901 ttttccgcga agcggttgcc gccattggct acccctgtat tgttaaaccg gtgatgagtt
      961 cttccggtaa agggcagacg tttattcgtt ctgcagagca acttgctcat gcatgggagt
     1021 acgctcagca aggtggtcgc gccggagcgg gccgcgtaat tgttgaaggc gtcgttaagt
     1081 ttgacttcga aattaccctg ctaaccgtca gcgcggtgga tggcgtccat ttctgtgcac
     1141 cggtaggtca tcgccaggaa gatggcgact atcgtgaatc ctggcaacca cagcaaatga
     1201 gcccgcttgc ccttgaacgt gcgcaggaga ttgcccgtaa agtggtgctg gcactgggcg
     1261 gttatgggct gtttggtgtc gagctgtttg tctgtggtga tgaggtgatt ttcagtgaag
     1321 tctcccctcg cccgcatgac accgggatgg tgacgctaat ttctcaggat ctctcggagt
     1381 ttgccctgca tgtacgtgcc ttcctcggac ttccggttgg cgggatccgc cagtatggtc
     1441 ctgctgcttc tgccgttatc ctgccgcaac tgaccagtca gaatgtcacg tttgataatg
     1501 tgcagaatgc cgtaggcgca gatttgcaga ttcgtttatt tggtaagccg gaaattgatg
     1561 gcagccgtcg tctgggggta gcactggcta ctgcagagag tgttgttgac gccattgaac
     1621 gcgcgaagca cgccgccgga caggtaaaag tacagggtta aacccggcaa aaaaacgcta
     1681 caaaaatgcc cgatcctcga tcgggcattt tgacttttac agcttagcgc cttctacagc
     1741 ttcacgcgcc agcttagtaa tgcggtcgta atcgcccgct tccagcgcat ctgccggaac
     1801 cagccaggaa ccaccgatgc acagcacgct tttcagcgcc aggtagt
//
LOCUS       DAWDRC010000696         1825 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21556_length_1825_cov_1.979661, whole genome shotgun sequence.
ACCESSION   DAWDRC010000696 DAWDRC010000000
VERSION     DAWDRC010000696.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21556_length_1825_cov_1.979661"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    490..499
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          500 a          454 c          454 g          407 t
ORIGIN      
        1 ctggacagat gctatcgatc atggcccgca aatgacggca atccgcgacg gcgtccgggg
       61 caaagataaa ctagatgtgc ccattaagtt catctggaac tacgcgggaa ataccctggt
      121 taatcagcat tctgacatca acaaaacgca tgaaattctg caggacgaat cgaaatgcga
      181 aatgattgtg gtcatcgaaa actttatgac ctcatcggca aaatatgccg acattctgct
      241 gccagaccta atgaccgttg agcaggaaga tattattcct aacgactacg ccggtaacat
      301 gggatatctc attttcctcc agcctgtcac cagcgaaaaa ttcgaacgca aaccaattta
      361 ctggatcctg agtgaagtcg cgaaacgtct tggacctgac gtctatcaaa agttcactga
      421 aggtcgcacg caggaacaat ggttacaaca tctgtacgcc aaaatgcttg ccaaagatcc
      481 ggcgttaccn nnnnnnnnnc attttgtcgc ctacaaagca tttcgtgacg accccgaggc
      541 aaatccactt aaaacgcctt ccggtaagat tgaaatttat tccagcaagc tggcggaaat
      601 tgcccgtacc tgggaactgg aaaaagatga agtgataagc ccactgccgg tttatgcctc
      661 aacctttgaa gggtgggact cccctgaacg tagtgccttc ccactgcaac tgtttggttt
      721 ccattacaaa tcccgtacgc actcgaccta cggcaacatt gatgtcctga aggcagcctg
      781 ccgtcaggaa gtgtggatca accctattga cgcacagaaa cgtgggattg ccaacggcga
      841 tatggtgcgg gtgtttaacc atcgtggcga agttcggcta ccagccaaag tgacaccacg
      901 cattctccct ggggttagcg ctatgggcca gggagcctgg cacgaggcca atatgtctgg
      961 cgataaaatc gaccacggcg gctgtgtgaa tacgctaacc actctgcgcc cttcaccact
     1021 ggcgaaggga aacccgcagc acactaatct ggtcgagatc gaaaaaatat aacccactac
     1081 aaccataaat tctggcatga cattttgttt gaaaagcaat aagtgagtaa tgatgaaaat
     1141 ccataccaca gaggcgctca tgaaggctga aatcagccgt agaagtctga tgaaaacctc
     1201 cgcacttggc agtctcgcgc tggcaagcag tgctttcact ttgccgtttt cccaaatggt
     1261 ccgagcggca caggcaccgg tagaagagaa agcggtctgg agttcctgca ccgttaactg
     1321 cgggagccgc tgtctgttac gtttgcatgt gaaagatgac accgtgtact gggtggagtc
     1381 tgatacgaca ggtgacgacg tctacggtaa tcatcaggtt cgggcatgtt tacgcgggcg
     1441 ctctatccgc cgacggatga atcatcctga caggttgaaa tatcccatga agcgcgtcgg
     1501 caagcgcggt gaaggtaaat ttgaacggat aagttgggac gaagccctgg ataccatcag
     1561 tgataatctt cggcggatcc taaaagatta cggcaatgag gctgtacatg ttctctatgg
     1621 aacaggcgta gatggcggaa acatcaccaa ctcaaacgtc ccgtaccgtc tgatgaactc
     1681 ttgcggtggt tttctcagtc gctatggcag ctacagtacc gcacagatca gtgccgcaat
     1741 gagttatatg ttcggtgcca atgatggcaa cagcccggat gatatcgcca atacgaaact
     1801 ggtcgttatg ttcggaaata acccg
//
LOCUS       DAWDRC010000697         1821 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21610_length_1821_cov_1.905436, whole genome shotgun sequence.
ACCESSION   DAWDRC010000697 DAWDRC010000000
VERSION     DAWDRC010000697.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21610_length_1821_cov_1.905436"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    421..430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    789..798
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1535..1544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          399 a          468 c          518 g          406 t
ORIGIN      
        1 tctgcgggtt acattgcaga tcaaacaggc ggctcatttt cgccagacac tcagcggcaa
       61 caggttctaa acctgcactt aaatttatgt gcagagtatg gcgggctata tccggaatag
      121 cagtcaccac gccgcgatat tcgcctaccg ccagactacg ggcataataa ctgtccgcga
      181 ccgtttccac gccgctcacc gcacgggcgg cgagaaatcc caacatccac gaccagtcat
      241 acggcggctg ccagttcagg gtatacatcg catctccttg ttaatccgct ttcagcataa
      301 acgttattca gacgttacgc tttgctttca tattccggtt gtcgcgacgg caacatttcg
      361 ctaaagtcac gccccttctt caccggcatg gggattattt cgtgtttatt ggttttgatt
      421 nnnnnnnnnn cagcaaactg ttcagtggcg gtaatgcgcg acggtaaacc gcatttgcta
      481 aaaatggaaa acgacagcac gctgctgcct tcaatgcttt gcgcgccaac gcgtgaagcg
      541 gtaagcgaat ggctgtaccg ccatcatgat gttccggcag acgacgatga aacgcaggcg
      601 ctgttgcgtc gggcgattcg ttataaccgc gaagaagata tcgatgttac ggcgaaaagc
      661 gtgcagttcg gtctttcctc actggcacag tacattgatg atccagaaga agtgtggttt
      721 gtgaaatcac caaaatcgtt cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg
      781 tttgaggann nnnnnnnncg gtgatgaagc aaacacccag gcgcaaggga ttctggaacg
      841 cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag tacgagccgg tcgcggctgg
      901 gctggattac gaagccacct tgcaggaaga aaaacgagtg ctggtagtgg atatcggcgg
      961 tggtacgact gactgttcat tgctgctgat ggggccgcaa tggcgtgcgc gcctcgatcg
     1021 tgaagccagc ctgctgggtc atagtggttg ccgtattggt ggtaacgatc tggatatcgc
     1081 actggcgttt aagaacctga tgccgttgct gggcatgggt ggcgaaaccg aaaaaggcat
     1141 cgccctgccg attctgccgt ggtggaatgc ggttgccatc aacgacgttc ctgcgcagag
     1201 tgatttctac agtagtgcta acggtcgtct gcttaacgat ctggtacgcg atgcccgtga
     1261 accggaaaaa gtggccctgt tacagaaagt ctggcgtcag cgtttaagct atcgcctggt
     1321 gcgtagcgca gaagagagca aaatcgccct ttcaagtgta gcggaaaccc gcgcctcact
     1381 gccgtttatc agcgatgaac tggctacgct gattagccag caagggctgg aaagcgccct
     1441 cagccagcca ctggcgcgga ttctggaaca ggtgcaactg gcgctggata acgcccagga
     1501 aaaaccggac gttatctacc tgaccggcgg tagcnnnnnn nnnntgccgg gcattccgat
     1561 tgcaggcggc gatgactttg gctccgtcac cgccgggctg gcacgctggg cggaagtggt
     1621 gtttcgttaa ttgaaaaatg gctcagcaac ggcttaaatc tgcctgcgcc tgagccagtt
     1681 tttctgccgc ctgacgtagc ggttccatca acggcatggt ccgtgctggc gcatgaaggc
     1741 ggaatcgctg caactctgtc gcaccgacag tcagcgccga ttccagccga tgtcgtttgg
     1801 cggctagttt tgccattacc g
//
LOCUS       DAWDRC010000698         1813 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21715_length_1813_cov_1.900455, whole genome shotgun sequence.
ACCESSION   DAWDRC010000698 DAWDRC010000000
VERSION     DAWDRC010000698.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21715_length_1813_cov_1.900455"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          442 a          436 c          466 g          469 t
ORIGIN      
        1 ttcccaccgg caaaacgtga tttttactgg cgtgccagca ttacatccat cgcggcgaat
       61 ggggagtttt ctttgtttcc cggtatggaa aggatagtga cgttgctgga aggcggcgag
      121 atgttccttg aaagcgcaga ccgctttaac cataccttaa aaccgttaca acctttttcc
      181 tttgcagcgg acctggtggt aaaagcgaaa ctgacggcag ggcagatgtc gatggatttc
      241 aacatcatga cacgcctgga tgtctgtaag gcgaaagtca gaattgccga acgtacgttt
      301 actacctttg gttcacgcgg cggggtggtg tttgtaataa acggggcgtg gcaattgggc
      361 gacaaactgc tcactaccga ccagggcgcg tgctggttcg atggcaggca caccctgcgg
      421 cttttgcaac cgcaaggtaa actgttgttc agtgaaatca actggcttgc tggtcattca
      481 ccggatcaag ttcagtaatt tcatagttac cggaaagcag cggcttacaa agaattttat
      541 aaccgtcgtg atcaaacccg gcgggtgccc gaattaacgt tgtcgcttct ttcagcgaat
      601 taaccgcccc cagccacagc cagttttgaa taatatgata ttgagtcatt tccgggtgct
      661 gttctttcag cgctaccgca cctttccacg gccaacaaac gactcgtaaa cgttccagag
      721 actggcgcaa acgtagcgca tgatcgtcat ggctctcttt accgcagcat gctccggcgc
      781 aacgttttag cgctgaacga aaacatgcac gaccgcgact taacggctcc agtcccaaca
      841 ggccataaca aagtttttgt tcatcagcga tggtctgcaa tgcttgcaaa gctgcgcgcc
      901 tattggcaaa caggccaaac aggttggggg ctcgtgaaaa atccacctct ttggcataca
      961 ccacatcgac gcgcttttca tttaattgca gggcacagag ctggcgattg cgccgcaagc
     1021 gtttattaaa cagtggctgt tgttctttga ttaatcgcgc ttcaaggagc agagcgccga
     1081 tttcaccggc ggtacatatc cagctgatgc gtcgggattg ccgtagcatg gcggcttcat
     1141 ccggggtacg taaatgagaa aggacgcggc tgcggatgtt aatgcttttg ccgatataga
     1201 gcggcatggt gtcactttcg ccatgaaaca gatacacccc tggtcgggtg ggtaagtcat
     1261 taaggaatga acgtaaatgt tcgggatatt cataaattgc cgcagcttca aattcgagcc
     1321 gcggagaagt taaacgccgt accactgttg actccgaatg ctggttatct atccagtgta
     1381 acaggcggag agcgattaaa aaagaggcgg gtcgatgaca aaggttacac cgtttgacgt
     1441 gctacgcctg tcaggcctat tcgactcctg caatgtattg aatttgcata gttttgtagg
     1501 tcgagtaagg cgttcacgcc gcatccggcg tgaacaaagt actctttttc gtacaaacca
     1561 atatgtgcaa attattactt tttccagaaa tcatcgaaaa cggtaattgg cggacggcgt
     1621 ttatgttcgg ttttcagata ccagttctct attgttctgg cgacctgttc aggtacgttt
     1681 ttcccttcca gatagtcgtc gatattgtca taggtcacgc cgagtgccac ttcatctggt
     1741 agagaagggc gatcatcttc aagatcggcc gttggcgctt tcttataaag gtgttccggg
     1801 caacctaatg ccg
//
LOCUS       DAWDRC010000699         1812 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21728_length_1812_cov_2.384747, whole genome shotgun sequence.
ACCESSION   DAWDRC010000699 DAWDRC010000000
VERSION     DAWDRC010000699.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21728_length_1812_cov_2.384747"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          559 a          378 c          374 g          501 t
ORIGIN      
        1 cacaattcct gacagaagga atttctttga aaatccccgg atttataacg ggaaagagac
       61 aataacactg aagccagcgt ccacggatta tgtctataaa actgagtctg caagcaaaga
      121 taatggttac gttctgtcaa cgtatatgaa accggaatac tggtcgagaa cttcgtcagg
      181 atggaaaccg gtcagccggg agggcagaaa tgatgtggct tactgtgaat ttgtcactaa
      241 atatgcaaaa tcttttattc ctggtgaaca gcagatgcca gcacaactct atcagtctcc
      301 aacagggcat gagcttgaaa tcattccgtt atccgatata agtcgtttca gtgaaaatgt
      361 gaagctgaaa gttctgtata aaacgtcccc gctcgccgga gctatcatgg agcttgactc
      421 ggtcagttat ctgacatcat cccgtcatac tcatgcagtt gagcacaaac atcctgttca
      481 taaagcagaa ctcacctttg taactaatga ggatggtatc gtcacagtac cttctcttca
      541 tatcggacag tggctggcga aagtccaaaa taagaaaagt tttcaggaca aaagcctgtg
      601 tgatgaaact gtcgatgtgg caaccttaag cttctcccga aattaatgat actattcctg
      661 ccctgtattt tacgggcagg gtatatcaac cggaaatagc ctactgacat ccatgtaaaa
      721 aaactcataa ttattttgtt gatgtatctt acctaataca ttgatagctc cagtttttat
      781 accagagcat tctcgttgtc cctatgtact tgtctgcaat atcactgata tgcaggaata
      841 atattcttgg catggagtca gttccccgtc atcttaggta tcactttaac tcacaggtgt
      901 ccagtaaata tgaacaggat cccagtaatc attatcaatg gtttccttgg cgcaggaaaa
      961 accactctta tgaaaaacct gctcacacag gcaaacagga atcacttagc tgtatctgtc
     1021 attgtcaatg atatgagcga tctggatgtt gatggtgtac ttattgcaaa tacagaaatc
     1081 gttaatattg cgaacaataa tttcgtgagt atcacatctg atagtatcag cagtcagagt
     1141 gggattaaga cggtcgacaa ggcaattaaa aatatgctca ggcattattt gcctgacgtt
     1201 atatttgtcg aaacatccgg aggtagccac ccactgccat taattaagta tctgcagact
     1261 catgagcgat tacagctaaa atcactttta tcacttgtcg ataccgttat gctcaatgat
     1321 gactacaacg gagcgaacgc actgatacct gcatttcagg agaatctttt ccatggcaag
     1381 cgcagaatag agaatttact ggctgagcaa atcatgtttt gtagcacttt gctgttaacc
     1441 aaaaaggatc gactgtcatt cgacattgtc accgatgtgg cgaaagcaat ccatcctctg
     1501 aatccttacg taaatgtgat agctgtgtca tgggggaacc ttaaactagc agagttacta
     1561 acccttccgg attacgattt taaccgggtc ggattactta tcagagaact ggaagacaca
     1621 atcacagtag aagaagcaaa aatttcagcc caaaatggcg aaataatttc gcgagtgatt
     1681 aaagatgaca ggccattcca cccacaaaga ttatgggaaa cctatcatta ttttatggga
     1741 atgggtattt atcgtagtaa aggctttttc tggctaccag gaagacctga catggctctg
     1801 ctctggaacc ag
//
LOCUS       DAWDRC010000700         1799 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21903_length_1799_cov_1.978211, whole genome shotgun sequence.
ACCESSION   DAWDRC010000700 DAWDRC010000000
VERSION     DAWDRC010000700.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21903_length_1799_cov_1.978211"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          432 a          421 c          486 g          460 t
ORIGIN      
        1 tcccgcacga tcatcccctg gcaaataacc cgagtgtaac gctggctgaa ctggcgaaag
       61 aaccctttgt cttttttgat ccgcacgtcg gaacagggct gtatgacgat attctcgggc
      121 tgatgagacg ttacaatttg acgcccgtca tcactcagga ggtgggtgag gcaatgacca
      181 tcatcggtct ggtttccgct ggtctgggtg tttcaatttt gcctgcgtca tttaaacgtg
      241 ttcagctcaa cgaaatgcgc tgggtgccga ttgctgaaga ggatgcggtt tctgaaatgt
      301 ggttggtctg gccgaaacat catgaacaaa gtccggctgc gcgtaacttt cgtattcatc
      361 tgctgaatgc tctcaggtga gggaaatttc agcgaaaaag cccgaaaaat gtgctgttaa
      421 tcacatgcct aagtaaaaat ttgacgacac gtattgaagt gcttcaccat agcctacaga
      481 ttatttcgga gcgcgaaaat atagggagta tgcggtggtt gctgaaaatc agcctgggca
      541 cattgatcaa ataaagcaga ccaacgcggg cgcggtttat cgcctgattg atcagcttgg
      601 tccagtctcg cgtatcgatc tttcccgtct ggcgcaactg gctcctgcca gtatcactaa
      661 aattgtccgt gagatgctcg aagcacacct ggtgcaagag ctggaaatca aagaagcggg
      721 gaaccgtggc cgtccggcag tggggctggt ggttgaaact gaagcctggc actatctttc
      781 tctgcgcatt agtcgcgggg agattttcct tgctttgcgc gatctgagca gcaaactggt
      841 agtggaggag tcgcaggaac tggcgttaaa agatgactcg ccattgctgg agcgtatcat
      901 ttcccatatc gatcagtttt ttatccgcca ccagaaaaaa cttgagcgtc tgacttcgat
      961 tgccataacc ttgccgggaa ttattgatac ggaaaatggt attgtacatc gcatgccgtt
     1021 ctacgaggat gtaaaagaga tgccgctcgg cgaggcgctg gagcagcata ccggcgtacc
     1081 ggtttatatt cagcatgata tcagcgcatg gacgatggct gaggccttgt ttggtgcctc
     1141 acgcggggcg cgcgatgtga ttcaggtggt tatcgatcac aacgtggggg cgggcgtcat
     1201 tactgatggt catctgctac acgcaggcag cagtagcctc gtggaaatag gccacacaca
     1261 ggtcgacccg tatgggaaac gctgttattg cgggaatcac ggttgcctcg aaaccatcgc
     1321 cagtgtggac agtattcttg agctggcaca gctgcgtctc aatcaatcca tgagctcgat
     1381 gttacatgga caaccgttaa ccgtggactc attgtgtcag gcggcattgc gcggcgatct
     1441 actggcaaaa gacatcatta ccggggtagg cgcgcatgtc ggtcgcattc ttgccattat
     1501 ggtgaattta tttaacccac aaaaaatact gattggctca ccgttaagta aagcggcaga
     1561 tatcctcttc ccagtcatct cggacagcat ccgtcagcag gcccttcctg cgtatagtca
     1621 gcacattagc gttgagagta ctcagttttc taaccagggc acgatggcag gcgctgcgct
     1681 ggtaaaagac gcgatgtata acggttcttt gttgattcgt ctgttgcagg gttaacattt
     1741 tttaactgtt ctaccaaaat ttgcgctatc tcaatttggg ccaggaaagc ataacttag
//
LOCUS       DAWDRC010000701         1797 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_21932_length_1797_cov_1.934558, whole genome shotgun sequence.
ACCESSION   DAWDRC010000701 DAWDRC010000000
VERSION     DAWDRC010000701.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21932_length_1797_cov_1.934558"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1045..1054
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1493..1502
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          430 a          498 c          426 g          423 t
ORIGIN      
        1 gccgtcagac tcttcccgca gccgctggac ctctttgcga aaatgcagag ctcgctctac
       61 tgaacggtta atcagccgtt tgcccggatt accacgcagc atcgccgccg ccgtctcaac
      121 agaagcaaca atgggataac tgggcgaggt ggtggtatgc atcataaagg cttcgttaaa
      181 cgcctcttcg tcatactcac ctttaatgtg gatcagcgac gcctgcgata acgccgccag
      241 cattttgtgg gtcgactgcg tttcgaagat cacttttcct gcaacacgct cgccgctcat
      301 accactttta ccctggtaga ttggatgaaa atgggtgtac ggcacccagg cagaatcgaa
      361 gtgaatcgac gggacatcca gcgtctgttt gatccagtcg gtgttgtaga gcaagccatc
      421 ataggtggag ttggtgatca ccgcatgaac cggccattgt gcctgcgtgg tggcagcgac
      481 tttctcttcg atgctgtcgc gagtaaattc accgcgcggg atcccaccca gaatccccaa
      541 cgcattacgc gtcggtttca gccagactgg cactacatcg ttcatcatca acagatgcgc
      601 cagcgattta tgacaattgc ggtcgatcaa cagcgtactg ccggatggcg cggcgtacat
      661 acccacaatt ttgttcgacg tcgatgttcc attagtaacg atataactct gttccgcgcc
      721 aaacgtccgc gcgatgtact cttccgcttc caggtgtggc ccggtgtggt cgagcaacga
      781 accaagctcg gtgaccgaaa tagagacgtc cgccttgagg gtattcccgc cgaaaaaatc
      841 ataaaacaga cagccaaccg ggcttttttg atatgcggtg ccgcccatat gccccggcgt
      901 acaaaaggtg tacttccgct ctttgacgta ggtaaacaag gctttcgtga acggcggcgt
      961 aatgttatca agatattcgt tggtgtactg acgcatacga atggcgatat cttccgcctg
     1021 ccccagcgca tattcaaaaa accannnnnn nnnnccatcc gcatatcctg cacgctgaca
     1081 tccatcgtcg agtgggtgtt gatgaaggca taaagcggga gatattcatt aagttgattg
     1141 atatcgctac ataaatcgag actgtactca tcccagtcaa aaatcacgcc gcaaattcgt
     1201 gggttatgtt cgataaactt cagcaaatca acgctgtttt gtggccagat aatctgaaag
     1261 ccttgcgcca ccagcgccga ctccagttct ttgatgggct catctttata aaagacgcca
     1321 tgcggtccca taatggcaat gatgttcatg tgttcctcct ggaaaatcct tccttaatca
     1381 tagcctgctc aaaccgtggc gataaaaaag ggccaccgaa gtgacccttt tcagaacatt
     1441 tgccgattac gcgtagccgt agctcatcag gcgctgataa cgacgatttt ttnnnnnnnn
     1501 nntcagtttc agctctttca gacgcggagc gatgataccc atcgcttcag ccgccagcgg
     1561 cgctttgtca gcgctcttcc acagaatgga cgcacaacct tccggcgaga taacggaata
     1621 ggtgctgtat tgcagcatat tcactttatc gcccacgcca atcgccagcg caccaccaga
     1681 accaccttca ccgataaccg tacaaactac cggtacgccg aggcgagaca tttcacgcag
     1741 gttgcgtgca atggcttcag actgaccacg ttcttctgcg cccacgcccg gattagc
//
LOCUS       DAWDRC010000702         1788 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22026_length_1788_cov_2.033468, whole genome shotgun sequence.
ACCESSION   DAWDRC010000702 DAWDRC010000000
VERSION     DAWDRC010000702.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22026_length_1788_cov_2.033468"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          389 a          484 c          532 g          383 t
ORIGIN      
        1 ttcaaataca acctgagcgc cgacgccgcc ttccagcaat atgtgttgat gaccatcggc
       61 gacggactgg tggcgcagat cccttccctg ctgctctcca ccgcggcggc gattatcgtc
      121 acccgcgtca gcgacaacgg tgatatcgcc catgacgtgc gtaatcagtt gctggcaagc
      181 ccgtcggttc tctacaccgc cactggaatt atgttcgtgc tggcggtggt gccgggaatg
      241 ccgcatttcc cgttcctgat gttcagcgcc ctgcttggtt ttaccggctg gcggatgagc
      301 aagcgcccgc aagcggcgga ggcggaagag aaaagcctcg aaacgctaac ccgtacgatg
      361 acagaaacca gcgagcaaca ggtcagttgg gaaactattc cgctgatcga acctatcagt
      421 ttaagcctcg gctacaaact ggtggcgctg gtggataaag cgcagggcaa cccgctcacc
      481 cagcgtattc gcggcgtacg tcaggtgatt tccgacggca acggcgtgct gctgccggag
      541 atccgtattc gggaaaactt ccgcctcaag cccagccagt acgctatttt catcaacggc
      601 attaaggctg atgaagcgga tattccggcg gataaactga tggccctgcc ctccagcgaa
      661 acctacggcg agatagacgg cgtgctgggg aacgacccgg cgtacgggat gccggtcacc
      721 tggattcagc ccgcgcagaa agcgaaggcg ctgaatatgg ggtatcaggt gatcgacagc
      781 gccagcgtaa ttgccaccca tgtgaataag attgtgcgca gctatattcc tgatttgttt
      841 aactatgacg atattactca gctgcataac cgtttgtcgt cgatggcacc gcgactggcg
      901 gaagatttga gcgcggcgct caattacagc cagttgctga aagtgtaccg ggcgctactg
      961 actgaaggcg tttccctgcg cgatatcgtc accatcgcca ccgtgttggt cgccagtagt
     1021 gcggtgacta aagatcatat tctgctggcg gccgatgtgc gtctggcgct gcggcgcagc
     1081 attacccatc cgttcgttcg caagcaggag ctgacggtgt atacgctgaa taatgagctg
     1141 gaaaatctgc tgaccaacgt ggtgaatcag gcgcaacagg ccggcaaagt gatgcttgat
     1201 agcgtgccgg tggacccgaa tatgctcaac cagtttcaga tcacgatgcc gcaggtgaag
     1261 gagcagatga aagcggcggg gaaagatccg gtgctgctgg tgccgccgca gctgcgccct
     1321 ttgctggcgc gctacgcaag gttgtttgca ccggggctgc atgtgctgtc gtataacgaa
     1381 gtgccggatg agctggagtt gaagataatg ggggcgttga tgtaggcatc cggcatcggt
     1441 gcatattgcc ggatgcggcg tgaacgcctt atccggccta cgggtgagtg cggttttgtc
     1501 ggcatgataa gacgcgtagc ggcgcatctg gcaccggtgc acaaccgccg gatgcggcgt
     1561 gaacgcctta tccggcctac gggtgagcgc ggttttgtag gcatgataag acgcgtctgc
     1621 gtcgcatcag gcatcggcgc acagctataa aatgatacgc tccccagcac taaaatctgt
     1681 tacgtctccc gcccaatcga tgggatataa ccctttcgca acatcgcggt gaaacgttga
     1741 gaatggccaa tcactcactt gttttaccca accatgtttt actggatt
//
LOCUS       DAWDRC010000703         1779 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22130_length_1779_cov_2.203596, whole genome shotgun sequence.
ACCESSION   DAWDRC010000703 DAWDRC010000000
VERSION     DAWDRC010000703.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22130_length_1779_cov_2.203596"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    402..411
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          467 a          430 c          480 g          392 t
ORIGIN      
        1 gccaccacgc gcaggggcga ctgcgtcccg ctccgtgtag caaatacgac gcaaactctt
       61 gcttagttac aacatacttt aaagggatag tctcgtcatg accattaact tccgccgtaa
      121 cgcattgcag ttgagcgtgg ctgcgctgtt ctcttctgct tttatggcta acgccgctga
      181 tattccgcaa gtcaaagtga ccgtgacgga taagcagtgc gagccgatga ccattacggt
      241 taacgccggg aaaacacagt tcattattca gaaccacagc cagaaggcgc tggagtggga
      301 gatcctcaaa ggcgtgatgg tggtggaaga gcgggaaaat atcgcccctg gttttagcca
      361 gaaaatgacg gcgaatttac agcctggcga atacgatatg annnnnnnnn ntctgctgac
      421 taacccgaaa gggaagttga tcgtcaaagg tgaagcaacg gcggatgcgg cgcaaagtga
      481 tgcgctgtta agtcttggtg gtgcaattac tgcatataaa gcgtatgtca tggcggaaac
      541 cacgcaactg gtgaccgaca ccaaagcctt taccgacgct attaaagcag gcgatatcga
      601 aaaagcgaaa gcactgtatg cgccgacgcg ccagcactat gagcgcattg aaccgattgc
      661 tgaactgttc tccgatctgg atggcagcat tgacgcccgt gaagatgatt acgagcaaaa
      721 agccgccgat ccgaaattca ccggtttcca ccgtctggaa aaagcattgt ttggcgataa
      781 caccaccaaa ggaatggata agtatgctga ccagctttat accgatgtgg tcgatttgca
      841 aaaacgcatc agtgaactgg ctttccctcc ttcaaaagtg gtcggcggtg cagccggact
      901 gattgaggaa gtggcagcca gcaaaatcag cggtgaagaa gatcgctaca gccacaccga
      961 tctgtgggat ttccaggcta acgttgaagg ctcgcagaaa attgtcgatc tgctgcgtcc
     1021 acaactacaa aaagcgaatc cggaactgtt ggcaaaagtc gatgccaact tcaaaaaggt
     1081 cgataccatt ctggcgaaat accgtactaa agacggtttt gaaaattacg acaaactgac
     1141 cgattctgac cggaatgcac tgaaaggacc gattactgcg ctggcggaag atctggcgca
     1201 acttcgcggt gtgctggggc tggactaagc gttatgcagt atgaagatga aaacggcgtg
     1261 aatgaaccgt cacgccgacg tttactgaaa gggattggtg cgctggcact ggcgggaagt
     1321 tgtccggtcg ctcatgcaca aaaaacgcaa agtgcgccgg gtacgctttc accggatgca
     1381 cgcaatgaga aacagccgtt ttatggtgag catcaggcag ggatcctgac accacaacag
     1441 gctgcaatga tgctggtggc gtttgatgtg cttgccagcg ataaagccga tcttgaacgg
     1501 ttgtttcgct tgttgactca gcgttttgct tttctgactc agggcggagc ggcaccagaa
     1561 acgccaaatc cgcgcctgcc accactcgat tccggcattc ttggcggcta cattgcgccc
     1621 gataatctca ccatcacgtt atcggtgggt cactcattgt ttgatgagcg ctttggcctt
     1681 gcgccacaga tgccaaaaaa gttgcagaag atgacgcgtt tccccaacga ctcgctggat
     1741 gcggcgttat gtcatggtga tgtgttgcta caaatttgc
//
LOCUS       DAWDRC010000704         1760 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22395_length_1760_cov_2.090909, whole genome shotgun sequence.
ACCESSION   DAWDRC010000704 DAWDRC010000000
VERSION     DAWDRC010000704.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22395_length_1760_cov_2.090909"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          374 a          520 c          437 g          429 t
ORIGIN      
        1 tctggaagac tttacgtacc agtttatagg tggtgccttt ctccgggctg atattttccg
       61 gcgctgcgat aatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatgg
      121 aacgggcgtc cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt
      181 taccgcgcag gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca
      241 taccggtacc aatcgcctca ccggtcgaca atgcaccaga ctctgcacgc aaccagccat
      301 cggaaccacg gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt
      361 caccgatggt ctgcggcgtg cgctgcccca tatcaatctg cgggttaaga cgttgataca
      421 gtttcgccag cgcttctgag aaggtcaggc ggttgctgtt aaacagatcc tgatgctgct
      481 cgtgctgttc agagagcaca tccagcagca tggcgtgcgt ttcacgtacg ttcacgttca
      541 gacgtacgct gttcacctgg ccaaacgata cgttctgcaa cccctggttg agcatacgga
      601 tacggttctg ctcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga
      661 tcgccagttt ctgttcacgg gaggttaatt cttcggtcag acggctaagc tcgatctcca
      721 tctgttcgat agcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac
      781 gcagatgctg ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt
      841 ccgacatccg cagcacgtcg cgcagatgtt cgttatctgc caccgccaga cgcagcgcac
      901 ctaacgcctt atccgacatg gaacgcaaat catcagcgga gagataagcc agctcacgac
      961 ggtgtaagcg acgctcaacg ccattatctt tcaccatgcg catcaccgca caccagcccg
     1021 ctttggcggt cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt
     1081 tacgggtcag gttgtccatc tccgcttcgc agaatgtaag cgctttttcc agttgattgc
     1141 gtcgtgaacg gttattgctc agttgcgcgt gcaactcgtc acggcgaata cgtgcccgct
     1201 cttctgcccc gctatcagca cgcacgccga tatcctgcaa ttcacgttgc agatcgttaa
     1261 gtagctcttt tttggtgtca tacgaacttt tcagcgaagc cagcacctgg ttgtactgat
     1321 cctactgcgc ggccggtccg cgcaacgctt cacgagcgcg ggtacgctcc gcttccgcct
     1381 gttccagacg ttcacgcagt ttttcgttga gatcgctgtt accgctaagc atttctgccg
     1441 agtcagagta gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct
     1501 gacgggcatc gcgctgcatc tgctgagagt acgcgtaatc ttcttttaac tgttcgaact
     1561 gttccgggtc gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa
     1621 actgctgaac aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga
     1681 cgcgatccgc caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg
     1741 ccgtaacgcc ctctttcgcc
//
LOCUS       DAWDRC010000705         1753 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22497_length_1753_cov_1.648999, whole genome shotgun sequence.
ACCESSION   DAWDRC010000705 DAWDRC010000000
VERSION     DAWDRC010000705.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22497_length_1753_cov_1.648999"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    226..235
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          486 a          335 c          456 g          466 t
ORIGIN      
        1 ctttaaaggc gatttacatg ctggtgagat tggcccggtt cgggtaaatg gtcgctggga
       61 cggtattcgt ctgcgcggta acgcctggtg gcctaaacaa tcactgactg tattccagcc
      121 gctggtgcca cccgactgga agatgaactt acgcgatggt gaactgtatg ctcaggttgc
      181 attttcagct gcgcctgaac aaggattccg cgcgggaggg cacggnnnnn nnnnncggcg
      241 tgttgaaagg cggtagtgcc tggatgccag ataatcaggt taacggtgtc gattttgtcc
      301 tgcctttccg ttttgccgat ggagcctggc atctggggac tcgcggcccc gttacgttgc
      361 gaattgccga agtgattaat ctggtgacag cgaaaaatat tacggctgat ttgcaaggtc
      421 gttatccgtg gactgaagaa gaaccattgc tgttgactga tgttagcgtc gatgtgttag
      481 gcggtaacgt gctgatgaaa caattacgta tgccgcaaca tgatccggca ctgttgcgac
      541 tgaataatct ctcatccagc gaactggtta gcgccgttaa tccgaaacaa ttcgccatgt
      601 ccggggcatt tagtggtgca ttgccgttat ggctaaacaa cgaaaaatgg atagtgaaag
      661 acggctggct ggcaaatagc gggccgatga ccttgcggct ggataaagac accgcagatg
      721 cggtggtgaa agacaatatg accgcgggtt cagcaattaa ctggttgcgc tatatggaaa
      781 ttagccgttc atcgacaaaa attaatttag ataatctcgg tttattaacc atgcaggcca
      841 atattacagg taccagtcgc gttgatggta aaagcggtac ggtaaatctt aattaccatc
      901 atgaagagaa tatttttacg ctgtggcgca gtttacgctt tggcgataat ctccaggcat
      961 ggctggagca gaacgcgcgt ctaccgggaa atgactgtcc gcaaggaaaa gagtgtgagg
     1021 ataaacaatg aaaattttac tggctgcgtt gacgtcatct tttatgctgg ttggctgtac
     1081 gcctcgcatt gaagtcgctg cacctaagga accgatcact atcaatatga acgttaaaat
     1141 tgagcatgag atcatcatca aggcagacaa agatgtcgaa gagctgcttg agacccgtag
     1201 cgatcttttt tgaggtgatg atgaaaagaa cattacttct ttgtgcattt cttgttggct
     1261 tggtaagcag caatgtaatg gcattgactc tggatgaagc cagaactcag gggcgggtag
     1321 gggaaacatt ttatggttat ctggttgcgc tgaaaacaga tgctgaaact gagaaattag
     1381 taaccgacat caatgccgaa cgtaaagcga gctatcaaca actggcaaag caaaataatg
     1441 tgtcggtgga tgatatcgcg aaactcgccg ggcaaaagct cgtagaacgg gccaaacccg
     1501 gggaatatgt acaagggata aacggtaaat gggtgcgaaa attttaatag caaaaccgca
     1561 atgtcgggat aacgctacag gagaagacga ttaatctatt ttccggaaac attttgctct
     1621 tttaattata aggatgaatt atgtttcggc tctaatttat ttattcgagt attgatttat
     1681 accgtttttt cattaaataa atatctatgt ggcgcatttg aatttataat ttatataata
     1741 tacataattt gta
//
LOCUS       DAWDRC010000706         1746 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22591_length_1746_cov_1.923714, whole genome shotgun sequence.
ACCESSION   DAWDRC010000706 DAWDRC010000000
VERSION     DAWDRC010000706.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22591_length_1746_cov_1.923714"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1057..1066
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          407 a          513 c          420 g          396 t
ORIGIN      
        1 atagaggaag cgagcaaagg tggtttcttt cgctttttcc agcgcgttaa ccacgttttg
       61 tgccgatttc ggccccatac gctccagtcc ggtcagtttg cctgcggtga gtttgaacag
      121 atccgccgga gtgtggacat attctttttc aaccagttga tcgatgattt tgtcgcccat
      181 tccgtcaaca tccatcgcac ggcgggaaac aaagtgtttc agagactctt tacgctgcgc
      241 accgcaaatc aggccaccgg tacagcgggc aaccgcttca ccttccacac gctcgacgtc
      301 agaaccacat accggacaat gcgtcgggaa tacaacctca cgggtatctt ccgggcgttc
      361 agaaagcacg acgttaacca cctgcgggat cacatcgcct gcgcggcgaa tcaccacttt
      421 atcgccaatg cgtaaaccaa gacgttcgat ttcgtcagca ttatgcagtg tagcgttact
      481 caccaacacg ccagcaacat ggacaggttc cagacgcgca acaggcgtaa tcgcgccagt
      541 acgcccaacc tgaaactcga cgtcacgcac aaaggtcatc tgttcctgcg ccgggaattt
      601 aaacgctacc gcccagcgcg gggcacgcgc gacaaagcca agctgctcct gctgtgccag
      661 tgagttgacc ttaatcacca cgccgtcgat atcaaagccc agcgtcggac ggtcttcttc
      721 tactttgtgg tagaacgcca gcacttcttc cgccgattca caaagcgtta cccgatcgct
      781 gaccggcaaa ccccactttt taaattgcag taaacggcca agatgagtat ccggcagctc
      841 gccaccttcc agaacaccga cgccgtagca gaagaaagtg agcggtcgct tcgctgtaat
      901 acgcggatca agctgacgca gtgaaccagc tgccgcatta cgtgggttag caaacacttt
      961 cccgcccgtg cgtcgcgcat cttcgttaat cttttcgaac cccgcctgcg gcaggaacac
     1021 ttcaccacgc acttccagac gcgccgggat attctcnnnn nnnnnncccc ggtggtgcca
     1081 tcgccacgag tcgcggcact gactaaaacg ccattttcat acagaatact gacggcaaga
     1141 ccatccagct tcagctcaca gcaccaggtg actttctcgt tgcttttcag acggtcctgc
     1201 actcgtttgt tgaaggcaag aaagctttct tcatcaaaaa cgttatccag cgacagcatt
     1261 ggcacttcat ggcggatctg gctgaaagcc gccagcggcg cagcacctac acgttgggta
     1321 ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca gctcgcgcag ttcgcgcatc
     1381 agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca tcacatgata aagatattca
     1441 tgatggcgaa gcgtcgttcg cagttctgtc agttgttgtt cgattgattc catatcgcac
     1501 catcaatgct aaaaaccccc gacaagcggg ggttcgaaga ggagttaatt tgccttaagt
     1561 gtatcagacg ttggcgtctt tgacttcgcg gatgatgtcc tggtactcgc gcaatttctg
     1621 cggagtcatc atacggcgct gatcgtcaag cactacaccg cccacttcat cggcaatatg
     1681 ctgcgcagat tgcagcatca gcttaaagtt ctgcagctcg tcaccgtaag acggtacctg
     1741 cataaa
//
LOCUS       DAWDRC010000707         1744 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22622_length_1744_cov_1.830077, whole genome shotgun sequence.
ACCESSION   DAWDRC010000707 DAWDRC010000000
VERSION     DAWDRC010000707.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22622_length_1744_cov_1.830077"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1032..1041
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          357 a          451 c          546 g          380 t
ORIGIN      
        1 caccgcgttc ctgatggaac agctggaact gcgcgaagaa ctggacgaga tcgaacaggc
       61 gaaagatgaa gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt ttgatacccg
      121 ccatcagttg atggttgaac agttagacaa cgagacgtgg gacgcggcgg cggataccgt
      181 gcgtaagctg cgttttctcg ataaactgcg gagcagtgcc gaacaactcg aagaaaaact
      241 gctcgatttt taatttctgg aagctaaaca tggccttatt acaaattagt gaacctggtt
      301 tgagtgctgc gccgcatcag cgtcgcctgg cggccggtat tgacctgggc acaaccaact
      361 cgctggtggc aacagtgcgc agcggtcagg ccgaaacgtt agctgaccat gaaggccgtc
      421 acctgctgcc atctgttgtt cactatcaac agcaagggca ttcggtgggt tatgacgcgc
      481 gtactaacgc agcgctcgac accgccaaca ccattagttc tgttaaacgc ctgatggggc
      541 gctcgctggc tgatatccag caacgctatc cgcatctgcc ttatcaattc caggccagcg
      601 aaaatggcct gccgatgatt gaaacggcgg cggggctgct gaacccggtg cgcgtttctg
      661 cggacatcct caaagcactg gcggcgcggg caactgaagc cctggcaggc gagctggatg
      721 gtgtcgttat caccgttccg gcgtactttg acgatgccca gcgtcagggc accaaagacg
      781 cggcgcgtct ggcggggctg catgttctgc gcttacttaa tgaaccgacc gctgcggcta
      841 ttgcctacgg gctggattcc ggccaggaag gcgtgatcgc tgtttatgac ctcggcggcg
      901 ggacgtttga tatttccatt ctgcgcttaa gtcgcggcgt gtttgaagtg ctggcgaccg
      961 gcggtgattc cgcgctcggc ggcgatgatt tcgaccatct gctggcggat tacattcgcg
     1021 agcaggcggg cnnnnnnnnn nttcctgatc gtagcgataa ccgcgttcag cgtgaactgc
     1081 tcgatgctgc cattgcggcc aaaatcgcgc tgagcgatgc ggtctccgtg accgttaacg
     1141 ttgcgggctg gcagggcgaa atcagccgtg aacaattcaa tgaactgatc gcgccactgg
     1201 taaaacgaac cttactggct tgtcgtcgcg cgctgaaaga tgcgggcgta gaagctgatg
     1261 aagtgctgga agtggtgatg gtgggcggtt ctactcgcgt gccgctggtg cgtgaacgag
     1321 taggcgaatt tttcggtcgt ccaccgctga cttctatcga cccggataaa gtcgtcgcta
     1381 ttggcgcggc gattcaggcg gatattctgg tgggtaacaa gccggacagc gaaatgctgt
     1441 tgctggatgt gatcccgctg tcgctgggcc tcgaaacgat gggcggcctg gtggagaaag
     1501 tgatcccgcg taataccacg attccggtgg cccgcgccca ggatttcacc acctttaaag
     1561 atggtcagac ggcgatgtct atccatgtga tgcagggtga gcgcgaactg gtgcaggact
     1621 gccgctcgct ggcgcgtttt gcgctgcgag gtattccggc gctaccggct ggcggtgcgc
     1681 atattcgcgt gacgttccag gtcgatgccg acggtctttt gagcgtgacg gcgatggaga
     1741 aatc
//
LOCUS       DAWDRC010000708         1741 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22661_length_1741_cov_2.818505, whole genome shotgun sequence.
ACCESSION   DAWDRC010000708 DAWDRC010000000
VERSION     DAWDRC010000708.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22661_length_1741_cov_2.818505"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          477 a          466 c          391 g          407 t
ORIGIN      
        1 aacaacctgg agaaccactc gtctatggct catttcagaa agacctgata gttaaaatca
       61 actaccacta aacagcaaaa tgccgggaac accccggcat tttcatattt agtatttcac
      121 gtcattagct ggctttacgc tcatgtgcct gacggtatgc caccaggtct tcaatagtca
      181 cgagcgccat attgtgttta ttggcaaact caatgcactc tggtgcacgc gccatcgtgc
      241 catcgtcatt agtcagctca cacagcacac cagctggttt aaagcctgcc agcgtcatca
      301 gatcgatagt cgcttcagta tgaccgccac gcgtcagtac accacctgcc tgcgcgcgaa
      361 gtgggaaaac gtggcccgga cgattcagat ctgacggttt tgcgccatcg gcaatcgctg
      421 cgcgaacggt cgtaatacgg tcagcggcag aaacaccggt agtcacacct tcagctgctt
      481 caatggtcac ggtaaaaccg gtgccatagg cgctggtgtt attttctacc atcattggca
      541 gatcgagttg tttacggcga tcgtcagtaa tgcacaggca aacaatacca ctcccgtggc
      601 gaatggtcag cgccatctgc tcaacggtca tggtttctgc cgggaagatc atatcgcctt
      661 cgttttcacg gtcttcatca tcaagcacca tcacaccgcg tccttcacgc agcgcagcca
      721 gtgcattttc aacacgttcg aaaggcgtac caaaagagga aagtagcgtc tgattcatgg
      781 taaaaaaacc tcactaaaat tatggttacc agaatcaggg cagtcttagg agtggcggca
      841 tatagccaaa ataacgtgag cgggtccatg cccgacagaa tcgttactct ctcccatccg
      901 gactctaacc gtcggccccg gaattacacc ggatctgctg tcctttgagt tcgcacccaa
      961 agcgctcgcg ggctttcaac tgagttgatt taccgccggt ggggaatttc gccccgccct
     1021 gagaataagc ggattcacta taacgctaat gattagcgcc agcaacgcat agcttcacat
     1081 aattctggtt tatgacttac ccttatcgca ctacaatggc actcaacacc tatcagtaca
     1141 gggaaactac aatgattgac ccgaaaaaaa ttgagcaaat cgctcgccag gttcacgaat
     1201 caatgcctaa aggaatcagg gagttcgggg aagatgtgga gaaaaaaatc cgccaaaccc
     1261 tacaagcgca gctgacgcgt ctcgatctgg taagccgcga agaattcgac gttcagacgc
     1321 aagtcctgtt acggactcgt gaaaaactgg cgttactgga gcagcgcatc agcgaactgg
     1381 aagcgcgcaa taatccgata gcagaacttc aatctcctcc ggctatcccg ccagttgata
     1441 aagcagaata aagcaccaca aaaacgggcc agacggcccg taatatcagt gttctaattc
     1501 aagttctctg gtttgcatca tctgttggca ataccaggca taacgtgcgc aaaaccaggt
     1561 tctatgttct tcatccatgt taccagtaac ggccagaata tcgaccgggc gaccttctgc
     1621 gtgcattctg gcaaaactac gcgcgagaaa atcgaaatta tttaaagaat taagactatg
     1681 atccataagc acatcctccg gttattgcat accttttaag gccatatgct ttttctgatt
     1741 t
//
LOCUS       DAWDRC010000709         1741 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22666_length_1741_cov_2.058719, whole genome shotgun sequence.
ACCESSION   DAWDRC010000709 DAWDRC010000000
VERSION     DAWDRC010000709.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22666_length_1741_cov_2.058719"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          471 a          411 c          395 g          464 t
ORIGIN      
        1 ggccggccca cgccatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga
       61 gttaaacagc tcagtggatg tctgggaact gaaagatccg catggcaata tcgaatgtgt
      121 ccagacgctg gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca
      181 tatcaccccg gatggtcgtc atttatacgc ctgcgaccgt accgccagtc tgattactgt
      241 tttcagcgtt tcggaagatg gcagcgtgtt gagtaaagaa ggcttccagc caactgaaac
      301 ccagccgcgc ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa
      361 atctcaccac atctcggtat acgaaattgt tggcgagcag gggctactgc atgaaaaagg
      421 ccgctatgcg gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat
      481 ttacccggcg cagtctcttc tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc
      541 tggcgttcga cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc
      601 aaggcaaaaa agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc
      661 aatgtccgac gtataaccgg ttccaccagc gggataccaa taatcgaaga atagggcgca
      721 tggggcattg ccagaccagg aagcgccgaa atccccagtc ccgcctccac cagccctaat
      781 gacgtcgaaa gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcgcctagc
      841 tgttgtacta tcaatagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaactt
      901 actaactcct gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggcc
      961 agcacgaacg gctcgttgac cagtggggta aaatcaatgg atgaatttgt gacgttgttc
     1021 atgtttatgc caaagtctga ttcgttacac agcaccgatt ccatgcaatt attcgtgcct
     1081 tgttccagaa tccgcacttt aatattggga tacagctcat taaatttacc gattgccagc
     1141 ggtaaaaaat aaaatactgc ggtcggaata cacgctaacg tcaccatacc acgatgataa
     1201 gcattcatat cacgaatatt aaaaagcgtt tcatcgaatt tttttattaa ttcccgcgcc
     1261 tccgggagca acctttttcc tgcttttgtt aacgtaacct tgcgagttgt acgctcaaat
     1321 agttgaatgt gtaaatcttc ctccatcttt tttattcttc gcgttaatgc aggttgcgta
     1381 atattgagta atttagcagc attgttaaat gaactggact ctgccagtat gacaaatgcc
     1441 ttcatacttg ataattcatg cttcattatt actccggaaa atggaagcga cgattttggg
     1501 tgactggcag ttaaaaattt taactgcatt tagccaactt aaattaatga aaaaatgtta
     1561 ttaatcgttg agctaaagtc attagagatg ctttgccctt aatgtaacca tatcgcaata
     1621 agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag
     1681 gtggaacctc gagggggcgc gttcctgtta gcggaacatc tacccgaaga tcaaacgcag
     1741 c
//
LOCUS       DAWDRC010000710         1735 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22750_length_1735_cov_1.826190, whole genome shotgun sequence.
ACCESSION   DAWDRC010000710 DAWDRC010000000
VERSION     DAWDRC010000710.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22750_length_1735_cov_1.826190"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1094..1103
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          385 a          466 c          372 g          502 t
ORIGIN      
        1 aaaaaacatc ttaactataa acacaacgcc attagttact ctcagtaact agtggcgttc
       61 atgtctaatg attgacccgc agtattatct ggtgcttgtc aatcagattt attcattgtt
      121 taatactacc tcagcatcct gttatacgct acacaaccat tattactcat cctttgagtt
      181 acatttaata tctgaaatat ttttgtgtta atcaatgtgt ggatatattc cctactaatt
      241 cactaattca tacagcacca agctagaaac attgttatag ttccccgcac aaagcaaacc
      301 attaaatttg attggatgtt ttcgagataa ccatcaaatc aagtaataac taatctccca
      361 tataccgcca acacccgctt catcgcggca ctctggcgac actccttaaa aatcaggttc
      421 gtgctcacct ttccttcccg ttcttctcta gtagcgaacc ggtaatacac cgttcgccag
      481 accttaccat caatgaccag gattcctgcc cgcgccattt ttgccgcagc ctgatttatg
      541 ctggttaatg tcacacctgt tgtcgcagca acgtccggcg tacagaagct cttgtgcgtc
      601 tccagataat gaataattgc ctctttgccc gtcatacagt tgctcctttc agtccgaact
      661 tcgctttgat ttctgcgatc ttcgccagag cctgtgcacg atttagaggt ctaccgccca
      721 tgacaggaag ttgttttact ggttcaggta tcgcctcacc acggttaatt cgcgcggtca
      781 tacaagtcag ttcatcggca gccttgcgcc gtaattccgc atcagtaagc gcattggccc
      841 gcatgttctg gtacaggttg gtaaccagcc agtagtgcgc gtttgatttc cacggataag
      901 actctgcatc cggatacagg ccacgcttcc gacaatactc atagaccata tcaaccagct
      961 cgctgacgtt tggcagcccg gcgttaacgg atgcttcttc ccggcaccat gcaacaaact
     1021 gcccgggtga tggcaggaat ggtcgattct gccgacgggc tacgcgcatt cctgcgttaa
     1081 cctgttccat cgtnnnnnnn nnngccggga aagttgccag taactggctg aacacaccat
     1141 tgataatctg cgctacctgc tgtacctgcg gcttttcgtc gtactgttcc ggcatgttat
     1201 tggcgatccg gcgcatctgc tcacggtcaa aattaaccat ctgtgcggcg atgtttttca
     1261 tagctccacc ccgtaaatcc agtcagtgtt cgtcaggtcg agttttggtt ttccggctgt
     1321 cacgccagcc tgttgcttgt tacggttgat ttcgagttgg gtccacttgt cgcggagttt
     1381 ggccggactt agcacgttac cggaccagaa gttgtcctgg catgcccagc ggaacagcac
     1441 gcacatgtcg cggtggttac gtccgtcacg ttcacgcatc aggcgaatat cgttagccca
     1501 ccctgcaaaa ttcggttttc tggctgatgg cgcgatggtc ttcaccatgt caaacatcca
     1561 ctctgcggcg gtcaggtctt ctgctgtccc ccacttgctg ccgctctgaa tcgcagcatc
     1621 cggtttcacc acaggaaggg cattttctgg ctggtcagag gattcgtcag aattctcgga
     1681 cgaaaaaggt tttatattgt cttttgttag tttgtctttt gtgtttacct gattc
//
LOCUS       DAWDRC010000711         1734 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22761_length_1734_cov_2.172126, whole genome shotgun sequence.
ACCESSION   DAWDRC010000711 DAWDRC010000000
VERSION     DAWDRC010000711.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22761_length_1734_cov_2.172126"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1240..1249
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          431 a          435 c          489 g          369 t
ORIGIN      
        1 tgggcaactg aaatgggagc tggaagatta ctgcttccgt tatctccacc cgaccgaata
       61 caaacgcatc gcaaaactgc tgcatgaacg ccgtctcgac cgcgaacact atatcgaaga
      121 gtttgtcggc catctgcgcg ctgagatgaa agctgaaggc gttaaagctg aagtgtatgg
      181 tcgaccgaaa cacatctaca gcatctggcg caaaatgcag aaaaagaacc tcgccttcga
      241 tgagctgttt gatgtgcgtg cggtacgtat tgtcgccgag cgtttacagg attgttatgc
      301 cgcactgggg atagtgcaca ctcactatcg ccacctgccg gatgagtttg acgattacgt
      361 cgctaatccg aaaccaaacg gttatcagtc tattcatacc gtggttctgg ggccgggcgg
      421 caaaaccgtt gagatccaaa tccgcaccaa acagatgcat gaagatgcag agttgggtgt
      481 tgctgcgcac tggaaatata aagagggcgc ggctgctggc ggcgcacgtt cgggacatga
      541 agaccggatt gcctggctgc gtaaactgat tgcgtggcag gaagagatgg ctgattccgg
      601 cgaaatgctc gacgaagtac gtagtcaggt ctttgacgac cgagtgtacg tctttacgcc
      661 gaaaggtgat gtcgttgatt tgcctgcggg atcaacgccg ctggactttg cttaccacat
      721 ccacagtgat gtcggacacc gctgcatcgg ggcaaaaatt ggcgggcgca ttgtgccgtt
      781 cacctaccag ctgcagatgg gcgaccagat tgaaattatc acccagaaac agccgaaccc
      841 cagccgtgac tggttaaacc caaacctcgg ttacgtcaca accagccgtg ggcgttcgaa
      901 aattcacgcc tggttccgta aacaggaccg tgacaaaaac attctggctg ggcggcaaat
      961 ccttgacgac gagctggaac atctggggat cagcctgaaa gaagcagaaa aacatctgct
     1021 gccgcgttac aacttcaatg atgtcgacga gttgctggcg gcgattggtg gcggggatat
     1081 ccgtcttaat cagatggtga acttcctgca atcgcaattt aataagccga gtgccgaaga
     1141 gcaggacgcc gccgcgctga aacagcttca gcaaaaaagc tacacgccgc aaaaccgcag
     1201 taaagataac ggtcgtgtgg tggtagaagg ggtcggtaan nnnnnnnnnt cggtaacctg
     1261 atgcaccaca tcgcgcgctg ctgccagccg attcctggag atgagattgt cggcttcatt
     1321 acccaggggc gcggtatttc agtacaccgc gccgattgcg aacaattggc ggaactgcgc
     1381 tcccatgcgc cagaacgcat tgttgacgcg gtatggggcg agagctactc cgccggatat
     1441 tcgctggtgg tccgcgtggt ggctaatgat cgtagtgggt tgttacgtga tatcacgacc
     1501 attctcgcca acgagaaggt gaacgtactt ggcgttgcca gccgtagcga caccaaacag
     1561 caactggcga ccatcgacat gaccattgag atttacaacc tgcaagtgct gggtcgcgtg
     1621 ctgggtaaac tcaaccaggt accggatgtt atcgacgcgc gtcggttgca cgggagttaa
     1681 tcttacatag ctggatatgg cgtcagccgt atccagcgat atcttttact cgaa
//
LOCUS       DAWDRC010000712         1731 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22811_length_1731_cov_1.478520, whole genome shotgun sequence.
ACCESSION   DAWDRC010000712 DAWDRC010000000
VERSION     DAWDRC010000712.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22811_length_1731_cov_1.478520"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    997..1006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          388 a          476 c          442 g          415 t
ORIGIN      
        1 cgcgcggtat cgcacatttc ccgcgcctgt tctggtgtca tggcgggcgg tttttcgcac
       61 atcacatggc agcccgcttc cagcgccatc agggtatgtt cgtaatgaaa acggttaggt
      121 gagcagacgc taaccacatc aggtttcacc gccagcagca tggcctgcgg atcgtcccac
      181 acggatgcat tcccgtattt ttctgccagc gcctgcgcct gggaaaggcg gctgtcacag
      241 acagccacca gttccagatc gttgctggtg cagtagtacg aagcatgaac tttatccgcc
      301 acctggcccg cgcctatgat cgcgactcgc agcggagagc ttgtcattgc acttttcaca
      361 aagacctctt agcaggtgcg caaccaggcc aacgaatcac ggtacgcctg ggcaggattt
      421 tctgcccgga tacgcccttc atacaccaca tagccctgat agttatcggc gcacagctgt
      481 tcaaacagcg cgtggaaatc gagggtgccg ctgcccggtt ggtagcgatg gttatcggca
      541 atatgcacat gaccgagcaa gtcgcggttg tcgtgcaacg cctgcgccag gttatcttct
      601 tcgatattca tgtgatagaa atcgccgata atctgtacat gttaagatcg ttttcgacga
      661 tgtaacgacg agcatcggcg agggtgttga tcatgtgatc ctgatagcgg tttaacggtt
      721 cgaggtacac cacggttccg gtacgtgcgg ccgcctgttc cagtacgcgc agggaatcac
      781 tcaccatttt gcggtcgccg tccaggctac gcggcgaggt catcggcggt aagcggaagg
      841 taaacatgcc ccatgcagcc ggaacgacga tacctttacc gcccacttct gccagcgctt
      901 caaattcagc ttccgacgca aaagcatttc caataagaat atcatcgact ccaccagttg
      961 ccaacagtat cctggcctgc agatcaatcg gcagatnnnn nnnnnngtaa accggtttct
     1021 ttgattgctg ctttgacttc ttcgagattg ttgaccagca gtttgccgtc aatctcaaaa
     1081 ccatcgaaac ccatctcttt gatataacga aatttctcca gaatgttttc cggaaagaac
     1141 gcctgattct gtgtgccgat tttcattctt ttcattcctt tgaattaaaa cgtgacgccc
     1201 attttgatgc tctgttccgg atgctggtcg acatactgca tgtagctttc cgggctgttg
     1261 gcaaaggtca ccaccggatc gattaaatct tcgcaattga gataaccgtt catcagcagt
     1321 tcccagcagg tttcttcaat acgcttacgg ctccagcgcg gataatccgg gttcggctcg
     1381 ctgcacgcac gggagaaaac aattttggcg ttattgaaat gggcttcgcg tccgaggtta
     1441 aaaccttcgg caaacggttt ggcaaacgcg acataggaga tggtgccgcc ataggccaga
     1501 ccgcggagtg ccgattgcag cgcgtcggca tagccactgg tttcgataat cacatcggca
     1561 ccctgcttgc cggtcagcgt tttgatctct ttacctacgt cagcgccaat ggggttaaga
     1621 cagaaatccg caccgtggcg acgggcaata tcacagcgat gggcgatagg atcgacgcca
     1681 atcaccaccg acgcgccagc gcgttttgcc agttgaatgg caatttgacc a
//
LOCUS       DAWDRC010000713         1724 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22886_length_1724_cov_2.524266, whole genome shotgun sequence.
ACCESSION   DAWDRC010000713 DAWDRC010000000
VERSION     DAWDRC010000713.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22886_length_1724_cov_2.524266"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          444 a          412 c          454 g          414 t
ORIGIN      
        1 ccctgtacga cagcgaaaaa gatgcatatc tgattggtcg cgaccatctg gggatcatcc
       61 cactgtatat gggctatgac gaacacggtc agctgtatgt ggcctcagaa atgaaagccc
      121 tcgtgccagt ttgccgcacg attaacgagt ttccggcggg gagctatttg tggagtcagg
      181 acggcgaaat ccgttcttat tatcatcgcg actggttcga ctacgatgcg gtgaaagata
      241 acgtgaccga caaaaacgag ctgcgtcagg cactggaaga ttccgttaaa agccatctga
      301 tgtctgatgt gccttacggt gtgctgcttt ctggtggtct ggattcctca attatttccg
      361 ctatcaccaa gaaatacgcc gcccgtcgcg tggaagatca ggaacgctct gaagcctggt
      421 ggccgcagtt gcactccttt gctgtaggtc tgccgggctc accggatctg aaagcagccc
      481 aggaagtggc aaaccatctg ggcacggtgc atcacgaaat tcacttcact gtacaggaag
      541 gtctggatgc catccgcgac gtgatttacc acatcgaaac ttatgatgtg accactattc
      601 gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg
      661 tgctgtccgg tgaaggttct gacgaagtgt ttggcggtta tctctacttc cacaaagcgc
      721 cgaacgctaa agagttgcat gaagagacgg tgcgtaaact gctggccctg catatgtatg
      781 actgcgcgcg tgccaacaaa gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc
      841 tcgacaaaaa attccttgat gtggcgatgc gcattaaccc acaggataaa atgtgcggta
      901 acggcaaaat ggaaaaacac atcctgcgtg aatgttttga agcgtatctg cctgcaagcg
      961 tggcctggcg gcaaaaagag cagttctccg atggcgtcgg ttacagttgg atcgacaccc
     1021 tgaaagaagt ggcagcgcag caggtttctg atcaacagct ggaaactgcc cgcttccgct
     1081 tcccgttcaa cacgccgacc tcaaaagaag cgtatctgta ccgggagatc tttgaagaat
     1141 tgttcccgct tccgagcgcc gctgagtgcg tgccgggcgg tccttccgtc gcttgttctt
     1201 ccgctaaagc gattgagtgg gatgaagcgt tcaagaaaat ggacgatccg tctggtcgcg
     1261 cggttggcgt tcaccagtcg gcatataaat aagacgtgta atattgcccc ggagaaatcc
     1321 ggggcaattt cagttactgg ggcgcttgtt gattctgata tgcagtaagg aaatgttccg
     1381 ggtttgccac atcgctgttt gacaactcat tactgtaggc cagtatttca tagattaatt
     1441 tcccaccact attgttgaat tccgaatttc atcctgtatg acaaaagaat ggctgtaggg
     1501 tggggaaaaa acacaaagta aacaataatt gacgaatata gcgccatgct gttcgcaacc
     1561 taaccaaaca gtcactttcg agcaattttc cttgaaaaag aggttgacgc tgcaaggctc
     1621 tatacgcata atgcgccccg caacgccgat aaggtatcgc gaaaaaaaag atggctacgt
     1681 agctcagttg gttagagcac atcactcata atgatggggt caca
//
LOCUS       DAWDRC010000714         1718 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_22966_length_1718_cov_2.189417, whole genome shotgun sequence.
ACCESSION   DAWDRC010000714 DAWDRC010000000
VERSION     DAWDRC010000714.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22966_length_1718_cov_2.189417"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    455..464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    815..824
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1324..1333
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          376 a          446 c          469 g          397 t
ORIGIN      
        1 atcccgaaaa ccttcttccc ggagcaggac accggcgtac tgatgggcgg gatacaggct
       61 gaccagagta tttcgtttca ggcgatgcgc ggtaagttgc aggacttcat gaaaattatc
      121 cgtgacgatc cggcggtgga taatgtcacc ggctttaccg gcggttcacg ggtgaacagt
      181 ggaatgatgt ttatcaccct taaaccacgc ggcgagcgga gcgaaacggc ccaacagatt
      241 atcgatcgct tgcgtaaaaa actggcgaaa gaaccgggag cgaatctgtt cctgatggcg
      301 gtacaggata ttcgcgttgg cggacgtcag gctaacgcca gttaccagta cacgttgtta
      361 tccgacgacc tggcggcact gcgcgaatgg gagccaaaaa tccgcaaaaa actggcgaca
      421 ttgccagagc tggctgatgt taactccgat caggnnnnnn nnnnatggcg cggagatgaa
      481 ccttatttac gatcgcgaca ctatggcgcg attgggaatt gacgtgcaag ccgccaacag
      541 cctgttaaat aacgccttcg gtcagcggca aatctcgacc atttaccagc cgatgaacca
      601 gtacaaagtg gtgatggaag ttgatccgcg ctatacccag gacatcagtg cactggaaaa
      661 aatgttcgtt atcaataacg aaggaaaagc gatcccgctg tcgtatttcg ctaaatggca
      721 accggcgaac gctccactgt cggtgaatca tcagggatta tcggcggcct cgaccatttc
      781 gttcaacctg ccgaccggga aatcgctctc ggacnnnnnn nnnngatcga tcgcgcaatg
      841 acccagcttg gtgtgccttc gacggtgcgc ggcagttttg ccggaacagc gcaagtgttc
      901 caggagacga tgaactcaca ggtgatcctg attatcgccg ccatcgccac ggtatatatc
      961 gtactgggta tcctttacga gagttatgtg catccactga cgattctctc caccctaccc
     1021 tcggcgggcg tcggagcgct gttggcgctg gagctgttca atgccccgtt cagcctaatt
     1081 gcgctgatag ggatcatgtt attaatcggc atcgtgaaga aaaacgccat tatgatggtc
     1141 gattttgcgc ttgaagccca acggcacggt aacctgacgc cgcaggaagc tattttccag
     1201 gcctgtctgc tgcgttttcg cccgattatg atgactaccc tggcggcgct gtttggtgcg
     1261 ctgccgctgg tattgtcggg cggtgacggc tcggagttgc ggcaaccatt gggggtcacc
     1321 attnnnnnnn nnnatcacca ttgtcggcgg tctggtcatg agccagcttc tgacgctgta
     1381 taccacgccg gtggtgtatc tctttttcga ccgtctgcgg atgcgttttt cgcgtaaacc
     1441 taaacaagcg gtaaccgagt aaatgacaga tcttcccgac agcacccgct ggcaattgtg
     1501 gattgtggct ttcggcttct ttatgcagtc gctggacacc accatcgtta acaccgcgct
     1561 tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg tcattgtctc
     1621 ttatgtactg accgtggcgg tgatgctgcc cgccagcggc tggctggcgg acaaagtcgg
     1681 cgtgcgcaat attttcttta ccgccatcgt gctgttta
//
LOCUS       DAWDRC010000715         1711 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23077_length_1711_cov_2.404589, whole genome shotgun sequence.
ACCESSION   DAWDRC010000715 DAWDRC010000000
VERSION     DAWDRC010000715.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23077_length_1711_cov_2.404589"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          413 a          432 c          472 g          394 t
ORIGIN      
        1 cgctggttgg cggatgtatt gggctgattt tgtatttgct gtcgcagctc ccggccttgt
       61 tccccctcgg caatttatag aaatccatga aaaactaagt ggctaaccgc gagagagatc
      121 aaataaacca cactaaaaga atggtaataa tgtggcctct tttcattatc tcccgtggta
      181 cggggaagga aaatcatgtc acttgtcacc gatctacccg ccattttcga tcagttctct
      241 gaagctcgcc agaaaggctt tctcaccgtc atggatctca aggagcgcgg cattccgctg
      301 gttggcacct actgcacctt tatgccgcaa gagatcccga tggcagccgg tgcggttgtg
      361 gtttcgctat gttccacctc tgatgaaacc attgaagaag ccgagaaaga tctgccgcgc
      421 aacctctgcc cgctgattaa aagcagctac ggcttcggca aaaccgataa atgcccctac
      481 ttctactttt ctgatctggt ggtcggtgaa accacctgcg acggcaaaaa gaaaatgtat
      541 gaatacatgg cagaatttaa gcccgttcat gtgatgcagt tgccaaacag cgttaaggac
      601 gatgcctcac gcgcgttatg gaaagccgag atgctgcgct tgcataaaac ggtagaagaa
      661 cgttttgggc acgagattag cgaagatgct ctgcgcgatg ccattgcgct gaaaaaccgc
      721 gaacgtcgcg cactggccaa cttttatcat ttggggcagt taaatcctcc ggcgcttagc
      781 ggcagcgaca ttctgaaagt agtctacggc gcaaccttcc ggttcgataa agaggcgttg
      841 atcaatgaac tggatgcaat gaccgcccgc gttcgtcagc agtgggaaga aggccagcga
      901 ctgggcccgc gcccacgtat tttaatcacc ggctgcccaa ttggcggcgc agcagagaaa
      961 gtggtgcgcg cgattgaaga gaatggcggc tgggttgtcg gttatgaaaa ctgcaccggg
     1021 gcgaaagcca ctgagcaatg cgtggcggaa acgggtgatg tgtacgacgc gctggcggat
     1081 aaatatctgg cgattggctg ctcctgtgtt tcgccgaacg atcagcgcct gcaaatgctc
     1141 agccagatgg ttgaagaata tcaggtcgat ggcgtagttg atgtgatttt gcaggcgtgc
     1201 catacctacg cggtggaatc gctggcgatt aaacgtcatg tgcgccagca gcacaacatt
     1261 ccttatatcg ctattgaaac agactactcc acctcggatg tcgggcagct cagcacccgt
     1321 gtcgcggcct ttattgagat gctgtaagga gtggcagtga catattcgat tggcattgat
     1381 tccggctcaa ccgccaccaa agggatctta ctggctgacg gcgtgattac gcgccgtttc
     1441 ctcgttccaa caccctttcg cccggcaaca gcaattactg aagcctggga aactctgcgc
     1501 gaagggttag agacaacgcc gtttctgacg ctcaccggct acggacggca actggtggat
     1561 tttgccgata aacaagtgac ggaaatctcc tgtcacgggc tgggcgcacg gtttcttgag
     1621 ccagcaacgc gcgcggtaat cgacatcggt ggtcaggaca gcaaagtgat tcagcttgat
     1681 gatgacggta acctgtgcga tttcctgatg a
//
LOCUS       DAWDRC010000716         1711 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23081_length_1711_cov_2.019928, whole genome shotgun sequence.
ACCESSION   DAWDRC010000716 DAWDRC010000000
VERSION     DAWDRC010000716.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23081_length_1711_cov_2.019928"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          449 a          462 c          412 g          388 t
ORIGIN      
        1 taccagacca gagccaaacc cttgcggtaa tacgcgggta tcagcgggta aattaacaac
       61 tacccagtgc caccagccgg agccggttgg cgcatccggg tcatagcagg tgacaacaaa
      121 acttttcgtt cccacaggaa catcatccca cgccagatgc ggtgaaatat tatcgccatc
      181 gtaacccatg ccgttaaaga catgacgatg cggcagctta tcgccatcgc gcagatcgtt
      241 actgatgagt ttcattagaa tgcctccggg aaacctcggc cttcagaccg gggaggaaag
      301 gaggcggttt tccgactaac tgtactttgc ataatcacat tttcctcttt agtatgtgaa
      361 cacatgaaac gcgcatataa ataccggttt tacccgacaa ctgagcaggc tgagctttta
      421 gctcagacgt ttggctgtgt gcgcttcgtc tacaattcca tccttcgttg gcgtaccgat
      481 gcgtactacg agcgaaaaga aaagatcggt tatctacagg ccaacgctcg ccttacggcg
      541 ctcaaaaaag agcctgaata catatggctg aatgatgttt cctgcgttcc cctccagcag
      601 tcgttgcgcc accaacaagc cgcctttgct aacttctttg ccggacgagc tgcatatccg
      661 gctttcaaaa gcaaacggca caaacaggtg gctgagttca ctgccagcgc gtttaaacac
      721 cgtgacggcg agttgtatat agcaaagagc aagtcgccgc tggatgttcg ctggagtcga
      781 gaattaccat ctgcgccgtc aaccgttacc atttccagag atagcgctgg caggtacttt
      841 gtttcctgcc tgtgtgagtt tgaacctgta tcaatgcctg ttaccgctaa aacggtcggc
      901 attgatgtgg gcttaaaaga tttattcgtc accgataccg gattcaaaac cgacaatccc
      961 cgccacaccg ctaaatatgc gaagcgatta acgctgctac agcgacgttt aagcaggaag
     1021 caaaaaggct caagaaaccg tattaaagcc cgcttaaagg tcgcccgact ccacgcgaaa
     1081 atcgccgatt gccggatgga caatctgcac aagttgtccc gcaaactgat taacgaaaac
     1141 caagttgttt gcgtcgaatc cctcaaggtg aaaaacatga tccgcaaccc gaagctgtct
     1201 aaagcaatag ctgacgcagg ctggagcgaa cttgttcgcc agctccagta caaaggcaaa
     1261 tgggccgggc ggtcagtggt cgccattgac cagtatttac cgtcctcaaa atgctgtagt
     1321 tgctgcggtt tcaccatgca aaaaatgcct cttaatgttc gtaaatggca ctgccctgaa
     1381 tgcggcgcag tcaagagccc tcctttcttg tttgcagaaa gtgtagccag aaaccctcac
     1441 gctgacttcc cgttattggc aaaaaaatgt ttcatcctgc accgcacgat taaccgctgc
     1501 ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt aaatcagttt
     1561 gccaaaaggc cggatccaga caccctgttc gacaaagaat ttttgcagcg ctgccatatt
     1621 caccggacga gtggtttcga ccacgccgat tgcccccagt acgcgcacat cggcaaccat
     1681 ttcggcgtca caggcggggg ccagttgctc g
//
LOCUS       DAWDRC010000717         1709 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23105_length_1709_cov_2.370012, whole genome shotgun sequence.
ACCESSION   DAWDRC010000717 DAWDRC010000000
VERSION     DAWDRC010000717.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23105_length_1709_cov_2.370012"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    638..647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          485 a          354 c          386 g          474 t
ORIGIN      
        1 tggtacgctt taaatagtgt aatcataacc ttaattgtaa atcactgaca cttcttacag
       61 ttgtaagaat aacatcacaa atgtcgcggc tctctctgat taccgcgaac caatatttat
      121 tatattcttc ttttcgtcct cactacttac catgcagaaa ggaattggaa gtagcgatga
      181 aatgattttc agcacttgat acaggaattt aaaaatatcg agtgcagcta cgacgatatt
      241 ttgcagatta tttatagagg cgttatgcct taaataaacg ctaaattaac acatatttaa
      301 ttaactaata ggtattgcta tgtcaactcc acttcaagga attaaagttc tcgatttcac
      361 cggtgtgcaa tctggcccat cttgtactca aatgctggcc tggtttggcg ctgacgtcat
      421 taaaattgaa cgtcccggct ttggtgacgt aacgcgccac cagctgcgag atattcctga
      481 tatcgatgcg ctttacttca ccatgcttaa cagtaacaaa cgttctattg aattaaatac
      541 caaaacagcg gaaggcaaag aggtaatgga aaagctgatc cgcgaagctg atatcttagt
      601 cgagaacttt catccagggg ccattgatca tatgggcnnn nnnnnnnggg agcatattca
      661 agaaatcaat ccacgtctga tttttggttc gatcaaaggg tttgatgagt gttcgcctta
      721 tgtgaatgta aaagcctatg aaaacgttgc tcaggcagcg ggtggcgcgg catctactac
      781 aggtttttgg gacggtccgc cgctggtaag cgctgcagcg ttgggtgaca gcaacaccgg
      841 aatgcatttg ctgatcggtt tacttgctgc tttgctgcat cgcgaaaaaa cggggcgtgg
      901 gcaacgagtc accatgtcaa tgcaggatgc cgtgttgaac ctttgccgcg tgaaattacg
      961 cgaccagcag cgtctcgata aattgggtta tctggaagaa tacccgcagt atccgaatgg
     1021 cacatttggt gatgcagttc cccgcggtgg taatgcgggt gggggcggtc agcctggctg
     1081 gatcctgaaa tgtaaaggct gggaaaccga tcctaacgcc tatatttatt tcactattca
     1141 ggagcaaaac tgggaaaaca cctgtaaagc catcggcaaa ccagaatgga ttaccgatcc
     1201 ggcatacagt acagcccatg cccgacagcc acatattttc gatatttttg ctgaaatcga
     1261 aaaatacact gtcactattg ataaacatga agctgtggcc tatttgactc agtttgatat
     1321 tccttgtgca ccggttttaa gtatgaaaga aatttcactt gatccctctt tgcgccaaag
     1381 tggcagtgtt gtcgaagtgg aacaaccgtt gcgtggaaaa tatctgacag ttggttgtcc
     1441 aatgaaattc tctgccttta cgccggatat taaagctgcg ccgctattag gtgaacatac
     1501 cgctgctgta ttgcaggagc tgggttatag cgacgatgaa attgctgcaa tgaagcaaaa
     1561 ccacgccatc tgatattaac gggggcttct ggctcccaat ttataaaaat ttcgaggtta
     1621 ttaatcatgt cagatcaact tcaaatgaca gatggtatgc atatcatcgt tgaagcatta
     1681 aaacagaata atattgacac tatttatgg
//
LOCUS       DAWDRC010000718         1704 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23185_length_1704_cov_1.729533, whole genome shotgun sequence.
ACCESSION   DAWDRC010000718 DAWDRC010000000
VERSION     DAWDRC010000718.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23185_length_1704_cov_1.729533"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1185..1194
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          414 a          405 c          433 g          442 t
ORIGIN      
        1 acaaaatctt gaaagtttct tccctgagat tcccgttgaa tttcatatta ataaagtgaa
       61 cgagcattat catgtgcctt tgttactcag ccaatatggg tattcaacct atcgtggcag
      121 ttaatgcgta aatatgtaaa agctgatctc tgctactgtt agggaggtga ctccctgaca
      181 gattaacagt aaacggctct tgttggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa
      241 gaatcaattt ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg
      301 gcaggtgtta aaagcactgg gcatcagcgc agctgcattt tctttgcctc acgctgcgca
      361 tgccgatctg cttagctggt ttaaagggaa cgatcgtccg cccgcccccg caggaaaacc
      421 gctggagttc agcaagcctg ccgcctggca aaataacctg ccactgacgc cagcagataa
      481 agtctccggt tataacaact tctatgaatt cgggctggat aaagccgatc ccgcggctaa
      541 tgctggtagc ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc
      601 attgaccctc gatcatgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg
      661 tatgcgctgc gtggaagcgt ggtcgatggt ggtgccgtgg attggttttc cgctacacaa
      721 attgctggcg cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacgattta
      781 tgcgccggaa cagatgcctg gccagcagga ccgctttatc ggcggcgggc tgaaatatcc
      841 ttatgtcgaa ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtagg
      901 tgtttatggc aaggcgttac caccgcaaaa tggcgcgccg gtgcgactga ttgtgccgtg
      961 gaaatatggc tttaaaggga ttaaatcgat cgtcagtatt aagctgaccc gcgagcgtcc
     1021 gccaaccacc tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc
     1081 gcatgttgat cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat
     1141 tctcgatgtt cagcgccagc caacactact gtttaatggt tacgnnnnnn nnnnatggtt
     1201 acgccgacca ggtggcatcg ctgtatcgtg gcctggattt gcgggagaat ttctaaatgc
     1261 gtctgacagc aaaacaggtg atatggctga aagtttgcct gcatcttgcc ggattgttgc
     1321 cgtttctctg gctggtctgg gcgatcaatc acggtgaact gggtgccgat ccggtgaaag
     1381 atattcagca ttttaccggt cgcactgcac tgaaattttt gcttgcggcc ttgttaatca
     1441 cacctctggc acgctacgca aaacagccgt tattgatacg cactcgccgc ctgttaggct
     1501 tatggtgctt tgcctgggcg acactgcatt taaccagtta cgcattgctg gagttgggcg
     1561 tgaataatct ggcgttatta ggtaaggagt taattacccg gccttattta acgttaggaa
     1621 ttatcagctg ggttattttg cttgctttag cgttcacttc aacccagtcg atgcagcgaa
     1681 aactaggcaa acactggcaa cagt
//
LOCUS       DAWDRC010000719         1696 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23279_length_1696_cov_2.157221, whole genome shotgun sequence.
ACCESSION   DAWDRC010000719 DAWDRC010000000
VERSION     DAWDRC010000719.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23279_length_1696_cov_2.157221"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          424 a          420 c          458 g          394 t
ORIGIN      
        1 aaccgccgac cccctccttg taagggaggt gctctcccag ctgagctaat cacccccgct
       61 gtgtggagtc gcattatagg gagagttcaa aatgagtcaa cgcattttct aaagaaattg
      121 ttcgttcgtc gtaaatttaa gcaagatgat cgcaaaacag accgtgttgc gcaatttgtc
      181 aacgaaaaca ataatgcgta aggttgcaac ccgaactaca ttgaggaatc aggcgggagt
      241 gatagaatat cgcccactca atttttccag gatttgccgg ttgtcggcat ctttctaaac
      301 gtaaggccat ttcatgaaaa tcaaaactcg cttcgcgcca agcccaacag gctatctgca
      361 cgttggcggc gcgcgtactg ctctttactc ctggcttttt gcacgtaacc acggcggtga
      421 gttcgtgctg cgtattgaag acaccgatct tgagcgttcc acgccggaag ctatcgaagc
      481 cattatggat ggcatgaact ggctgaacct ggagtgggat gaaggtccgt actaccagac
      541 caaacgtttt gatcgctaca acgcggtgat cgatcagatg ctggaagagg gcacagctta
      601 taaatgctat tgctctaaag agcgcctgga agcgctgcgc gaagagcaaa tggcgaaagg
      661 tgagaagccg cgttatgacg gtcgctgccg ccacagtcat gagcatcacg ctgatgatga
      721 gccgtgtgtc gtacgttttg ctaacccgca ggaaggttct gttgtttttg acgatcagat
      781 ccgtggtccg atcgagttca gcaaccagga gctggatgat ctgattatcc gccgtaccga
      841 tggttcccca acctataact tctgtgtggt tgtcgatgac tgggatatgg aaatcaccca
      901 cgttatccgt ggcgaagacc atatcaacaa cacaccgcgc cagattaaca ttcttaaggc
      961 tctgaaagcg ccggtaccgg tttacgcgca cgtttctatg atcaacggcg atgacggtaa
     1021 aaaactgtcc aaacgtcacg gggcggtcag cgtaatgcag tatcgtgatg atggttatct
     1081 gccagaagca ctgctgaact atctggtgcg tctgggctgg tcccacggcg atcaggaaat
     1141 cttcactcgt gaagagatga ttaaatactt cactctgaat gccgtcagca aatctgccag
     1201 tgcgttcaac accgacaagc tgctgtggct gaaccatcac tacattaacg cgttgccgcc
     1261 ggagtatgtt gctactcact tacagtggca cattgagcag gaaaatatcg atacccgtaa
     1321 cggcccgcag ctggctgatc tggtgaaact gctgggcgaa cgctgcaaga cgctgaaaga
     1381 gatggcacgg agctgccgtt atttctacga agattttgct gagttcgatg ccgacgccgc
     1441 gaaaaaacat ctgcgtccgg tagcgcgtca gccgctggaa gtagttcgtg acaaactgac
     1501 tgcgattact gactggaccg ctgaaaacgt tcatcacgct attcaggcga cggcggatga
     1561 gctggaagtg ggtatgggta aagttggtat gccgctgcgt gtcgccgtaa ccggtgcggg
     1621 gcagtctccg gcgctggatg ttaccgttca cgcaatcggt aagacccgca gtatcgagcg
     1681 tatcaacaaa gcgctg
//
LOCUS       DAWDRC010000720         1674 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23566_length_1674_cov_2.043237, whole genome shotgun sequence.
ACCESSION   DAWDRC010000720 DAWDRC010000000
VERSION     DAWDRC010000720.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23566_length_1674_cov_2.043237"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1011..1020
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          404 a          415 c          423 g          422 t
ORIGIN      
        1 acggttatgc ggaagtcccg gaagagatga agcgcaaatg gcagcgcgcg ctcggcgagt
       61 acctcgaaaa acgtcagagc ctgcaaggtc tggtggtact aatggatatt cgccatccgc
      121 tgaaagattt ggatcagcag atgattgagt gggcggtaga cagcaatatc gccgtgctgg
      181 tactgctgac caaagcggac aaactggcaa gcggcgcacg taaagcgcaa ttgaatatgg
      241 tgcgtgaagc ggtgctggcg tttaacggtg atgtgcaggt tgaaacgttt tcttcgctga
      301 agaaacaagg cgtggacaag ctgcggcaga aactggatac ctggtttagc gagatgcagc
      361 ctgtagaaga aacgcaggac ggcgaataat tttcttgcct taatgcttgt gccggatgtg
      421 gcgtatccgg cccgtaaatt caatagatta caattctacc gttggcctga taaacatggc
      481 gtatcaggca ttacggatct tttctttcgc ccaataaaaa acgccccagt cattactgac
      541 tggggcggct aaaatattca gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag
      601 aacatcttac ctctgtaccc tacgcgacta actttactct tttttgttca gttcagaaag
      661 cattttttgt aatttttttt cagttgttgc ataggaattt ctaatagcca tcacaaaacg
      721 tgacagctta tgttgcttac ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc
      781 tgatcccagt tttcgccact ccccacttcc accagtaacg gcacatccag acgggtacag
      841 ttttccatca gttgatgaat ctgcttcgcg acggcatcga catcatcttt atggacttca
      901 aataccagtt catcgtgtac ctgcatgatc atacgcacac gcggttgctc agcctgtagc
      961 cacgcatcaa cggcaatcat cgcccgtttg ataatgtcgg cggcggttcc nnnnnnnnnn
     1021 ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg agcaccattg ctggatttga
     1081 tatccggcag atacagacgg cgtccgtcca gcgtttcaac gtagccctgc tctttcgcct
     1141 gagcacgggt gcgttccata tactccagca cgccagggta gcgttcgaag taaaggtcca
     1201 tgtacttctg cgcttcttta cgcggaatgt tcaattgccg cgccagaccg aaagcactca
     1261 tgccataaat cagaccaaag ttgatcgctt tcgcgctacg acgttgctcg ctggtgacgg
     1321 tttccagtgg caaaccaaac acttctgccg ccgttgcacg gtggatatct tttccttcgg
     1381 cgaatgcggt cagcaagcct ttgtcacgcg agagatgcgc cataatgcgc agttcaatct
     1441 gcgagtagtc cgctgagaca atcacataat cctctggcgc aataaacgcc tggcggatac
     1501 ggcgaccttc ttcgttacgc accggaatgt tttgcaggtt aggatcggtt gacgataaac
     1561 gtcccgttgc ggttactgcc tggtgataag aggtatgtac acgcccggtt ttcgggttga
     1621 tcatcaacgg cagcttgtcg gtgtaggtcg atttcagctt cgccagacca cgat
//
LOCUS       DAWDRC010000721         1672 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23589_length_1672_cov_2.087199, whole genome shotgun sequence.
ACCESSION   DAWDRC010000721 DAWDRC010000000
VERSION     DAWDRC010000721.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23589_length_1672_cov_2.087199"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          425 a          395 c          418 g          434 t
ORIGIN      
        1 ggaacgacga tggaacgcat gcgggctgct ttagaggcta tcatgccatt tactgaatct
       61 tccagcgcta cgcaggtcag tgggtcaaca cccagttttg ctgcgcagtc gagatatact
      121 tgcggatgcg gcttgctgta aggcaatttt tcagccgagg cgagggcatc gaaactgtcg
      181 cgtaagtcaa acatggtcaa cactttttcc agcatatgta gtggtgacgc ggaggccagt
      241 cccaccaata aaccttgttc tttgcataac gcaacggctt cacgcacgcc tggcaataat
      301 gggcgtgtct cttcaaccag tgaaatggca cgagcaataa cccgttctac tacttcctga
      361 cggcttggtc cattccaggg ttgccgggcg taccaaagat cgaccaccat atcgatgcgt
      421 aaacctaagg tgtccggcag ctcgttacga cgggagatat ccacccccag gcttgccatc
      481 acatccagtt cagctcgatc ccataaaggt tctgagtcga taagtaatcc atccatatca
      541 aaaattgcag caagaatctg acgcggggtt gacattacaa cctctccttt agagcattat
      601 atgtaaggta ttaagccgtg cttatgatta agcgcctatc atatcgcctt taaagaatgg
      661 gcgaaaacag atatctacag gtaaactaag tgaaacgacg cgtcttaatc aaggagaact
      721 catgacgtat caacaagctg gacgcattgc tgttttgaaa aggattttgg gttgggtgat
      781 ttttatacct gcattgatct ctacattgat ttctttgctg aaatttatga atacccggca
      841 ggaaaaccag gaaggcatta atgcggtcat gctcgacttt actcatgtca tgatcgatat
      901 gatgcaggcg aatacgcctt ttttgaatgt gttctggtac aactctccga cgcccaattt
      961 taatggtggc gtgaacgtta tgttctgggt gattttcatc ctgatttttg tcggactggc
     1021 gttgcaggat tccggagccc ggatgagtcg ccaggcgcgc ttcttgcggg aaggtgttga
     1081 agatcaactc attctggaaa aagccaaagg ggaagagggg ctaacgcgtg aacaaattga
     1141 gtcacgtatt gttgttccgc accatactat tttcctgcag tttttctcgc tgtatatcct
     1201 gccgcttatt tgtattgctg caggttatgt gttcttttct ctgcttgggt ttatttaatc
     1261 aaaaggccgc acggagcggc cattatcctc aacccatatc atgctgctaa taatctatcc
     1321 agtgactgct gagcaataac caaatgctga ccgccaaata accttgcacg atttaacagc
     1381 gtgtagagtt ggtaaaccgg ttgacgttca aggaaatcgg caggtagcgg ggatactgac
     1441 tgatagccgt catagatttg tggcggctgt tcagtatgca gcggtaacat cgccaggtcg
     1501 cactctcggt caccccagta acaggccggg tcgaaaatgt acgggccatc cggacccagc
     1561 gcacagttgc cggaccataa atcgccgtgc aacagagaag gctgcggttg atgcgaggcg
     1621 agacgctgct gaatatgccc gacgatggca tcgaaattgc cgaaagcgat cc
//
LOCUS       DAWDRC010000722         1671 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23611_length_1671_cov_2.033416, whole genome shotgun sequence.
ACCESSION   DAWDRC010000722 DAWDRC010000000
VERSION     DAWDRC010000722.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23611_length_1671_cov_2.033416"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          348 a          432 c          415 g          476 t
ORIGIN      
        1 gtggcgtggt ttgtgtcgga acttcgctgg tcgatttatt taaccgtttt ctgttcagtg
       61 ccaaaattat ttttctggtg gtgatgctgg tattgctgct accgcatatt cacaaagtga
      121 atcttttaac cctgccgttg caacagggac tggcactgtc tgcaatcccg gtgattttta
      181 cgtcgtttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat ggcaacattc
      241 gtaagctacg ctgggtgttt ataatcggta gtgcgatccc cctggtggca tatattttct
      301 ggcagctggc gacccttggc agcattgatt caacaacctt tatgggattg ctggctaatc
      361 atgccggatt aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct ccgcatgttg
      421 agctggcagt gcatttattt gctgatttag ccctcgccac gtccttcctc ggcgtagcgt
      481 taggcttatt tgattatctg gcagatttgt ttcagcgttc aaataccgtt gggggacgat
      541 tgcaaactgg tgcaattacc tttctgccgc cactggcgtt tgcactgttt tatccacgag
      601 gatttgtgat ggcgctgggt tacgccggtg tggcgctggc ggtactggca ttgattatcc
      661 cttcgctgtt gacctggcaa agcagaaagc acaatcctca ggcgggttac cgggtgaaag
      721 gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt ggcgtgcaat
      781 ttttgattgc ggcaggcttg ttaccagaag tggggtgatt agattcttat tgggtgccag
      841 aattaacgct gacacccaat ttgacctctt aatgcaggca gcactgctta aatttcttac
      901 cactaccgca cgggcaagga tcattacgcc ccggtttctc ttctgctttg atcggttgct
      961 gaacagcttt ttcctgcgga tgcgccatcc agtacgcatg tagatcaagc gccgccagtc
     1021 gaatggcatc tacgctctct tcaaacgctt ctggcgacat cttctctacc cgctcgaagt
     1081 tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg ttttaacgaa tcgggcaacg
     1141 ttgaccagtc agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg
     1201 tcagctcgct gccatcgact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt
     1261 cgttcagacg ctctgcggta tcggccatat gttgaaaagc caggttcata aagcgcgtca
     1321 tctctttctc tgacgcccag cgcggcacat agtcagcccc accccacacg gcaaccagcc
     1381 actgtgccgg ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa ccgtccagct
     1441 ccgccacatc aagaatggcg tggtcagtgt tgtatttggt cagaatatcg tccagccatt
     1501 ccaactcact ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc
     1561 gccagcacct atcttacatt tcggtccgta tcagagatac tttttgagtg gctttgccgg
     1621 tgattaaaaa ttaaggaggg tgtaacgaca agttgcaggc acaaaaaaac c
//
LOCUS       DAWDRC010000723         1668 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23655_length_1668_cov_2.220087, whole genome shotgun sequence.
ACCESSION   DAWDRC010000723 DAWDRC010000000
VERSION     DAWDRC010000723.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23655_length_1668_cov_2.220087"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    210..219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          421 a          408 c          486 g          343 t
ORIGIN      
        1 gtctgacaag caatgccgaa gcacgcgtaa acgccgtttt gcttcaggcg gaacaacaac
       61 tcgcccgccg cttacagata acgaaaccac gtttgctgga acgccaacag ttacgcgaac
      121 agcgtttgca aaggcagcgc cagcgacaat ttacccgcga ctggcaatct gcgctggaag
      181 cactggcggc gcttttaccc gaacatgccn nnnnnnnnna caacgataag ctggcagcag
      241 ggaacgctgg agatcaaggg gctgacaaca agcattgccg cgttaaacgc gctggaaacg
      301 tcactccgcc aggatgcttc ttttcatctc aatcagaggg gggccacgca gcaggatgcg
      361 cagggacgct ggcaatttga gtatcagtta acaaggaagg ttagcgatga acatgttctt
      421 tgactggtgg ttcgccacat cactccgcct ccgccagttt tgctgggcag tctggttgct
      481 ggtgttagtt acgctcattt ttctgtcatt gacacaccat gaagagagcg atgcattaat
      541 tcgactacgg gcaaatcatc accagcagtg ggctgcactg tatcgcctgg tagacaccac
      601 ttccttcagc gaagaaaaaa cgctgccctt ttcgccactg gattttcagt tatccggcgc
      661 gcaactggtt tactggcatc catccgcgca gggaggtgag ttggcgttga aaacgctgtg
      721 ggaagcagtg ccgtcggcat ttacacggct ggcagagcgc aacgtcagcg tgagccgttt
      781 ttcgttaagc gtggaaggtg atgacctttt gttcacgcta caactggaga cgccgcatga
      841 gggttaaacg ctggttgttg gcaggtattg cattgtgcct tttaaccggt atgcgtgacc
      901 cttttaaacc gccggaagat ctatgccgga ttagcgaact tagccagtgg cgctatcagg
      961 ggatggtagg gagaggcgag cgcatcatcg gtgtaataaa agatgggcaa aaaaaatggc
     1021 gacgggtgca gcaaaacgat gtgctggaaa acggctggac gatcttacag ctgacgccag
     1081 acgcactaac gctgggaacc gggacaaact gcgaaccgcc acactggttg tggcaacggc
     1141 aaggagatac aaatgaagca atggatagtc gcactactgt tgatgcttat acccggcgta
     1201 caggcggcaa agccgcaaaa agtgacgctg atggtggatg acgttccggt agctcaggtg
     1261 ttgcaggtgc tggctgaaca ggagaagttg aacctggtgg tttcgccaga cgtcagcggt
     1321 acggtgtcgt tacatttaac tgacgttccc tggaagcagg cactacaaac tgtagtgaaa
     1381 agcgccggat tgataacgcg ccaggagggc aacattctct cggtgcattc cgttgcctgg
     1441 cagaatgaca atatcgcccg ccaggaggcg gagcagacgc gggcgcaggc aaatctgccg
     1501 ctggaaaatc gcaatattac tctgcaatac gccgacgccg gagagctggc gaaagcgggg
     1561 gagaagctac tgagtgccaa agggagtatg accgtcgata aacgtaccaa tcgccttttg
     1621 ctgcgagata acaaaacggc gttaagcacg cttgaacagt gggtatcg
//
LOCUS       DAWDRC010000724         1665 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23701_length_1665_cov_2.437267, whole genome shotgun sequence.
ACCESSION   DAWDRC010000724 DAWDRC010000000
VERSION     DAWDRC010000724.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23701_length_1665_cov_2.437267"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    611..620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          383 a          515 c          395 g          362 t
ORIGIN      
        1 tctagcggga attcaccgag gaaatcgaaa cggcgccccg tcccctggaa gctttcaagc
       61 gcacgcaaaa tagcctcgtc gtcaactccc tcttccgtag caaccgcaac cgcagctgcg
      121 gcgttcagcg cgttatgacg acctggcgca ttcagggtga cgcgcatcgg ctctttgtcc
      181 tggcgcagca gcgtaaagtg cccctgcggg ccaatctgct gataatcttc tacacgcacg
      241 tcggcatctt cgctgaagcc gtaagtcgtg gtctgacgtc ccacacgcgg taacaattcg
      301 cggatcaccg gatcatcaac acacatcacc gcacgaccgt aaaacggcag gttgtgcaga
      361 aaattaataa aagtctgttt taaattctca aagtcgccct ggtaggtatc catgtggtcg
      421 gcttcgatat tggtgacaat cgccaccatc ggttgcagat gcaggaacga tgcatcactc
      481 tcatctgctt cggcaatcag gtaccgacca tgccccaaac gcgcatgaac ccccgccgct
      541 tttaccagcc caccgttaac gaaggttggg tcgagtcccg cttctgcgta gatgctagaa
      601 accatcgcgg nnnnnnnnnn tcggcaatac cacccatacc ggcaccaccg atgccgacaa
      661 aatgtatgtg ccgaacgcga cgcatttcgg gcacgatgga acgcagtttt gccaattgtt
      721 gtgtattcat tctttacgcc attaacttct taaattcata cgatgcaaaa ggcatcgcta
      781 caattacgcc cgggcagccc ggctcacttc atttgccact cgctcggtgg catccggaat
      841 ggatgcagcg cgggcgcgtt ctgccatggt taataagatt tctcgcgacc acccggccag
      901 ggtgttggcg acagcatcca cgctaagctg tgactgctcg ataattttgg ctgcgcccgc
      961 tttttccagc ggtagcgcat tccagtattg ctgacggtct ttatgttgaa acggcacaaa
     1021 caacgccggt agtcctgccg cggcgatttc actcaccgtt aacgcaccgg agcggcaaac
     1081 gacgacatcc gcccacgcat acgccgccgc catatcatca ataaattccg tcactttatg
     1141 ctgcggttgc cccgcttcgg catacgcctg ttcaacggat tgttgcgaac ctttgccgct
     1201 ctgatgccag atagtgactg aatcacccag tttcgcagca acctgcggca ttgtctgatt
     1261 aagaatgcgt gcgccctggg aaccacccac taccaacaca cgaaccggac cttcacgtcc
     1321 agccaaacgt tgctgcggca acggcagcgc cagcacatcg gtacgcaccg ggttacccac
     1381 cacttccgca ttaggaaaag cacctggaaa cgcctgcatc actttggtag caatcttcgc
     1441 cagccattta ttggttaagc ccgcaatacc gttttgttca tgaagtacaa ccggaatgcc
     1501 taacgaccac gcggccagac cacctggacc tgatacatag ccgcccatac cgagcaccac
     1561 gtcaggtttg tacgctttca taatcgcctg cgcctgacgc caggcgttga agatacgcag
     1621 cggcgcagct atcagtgctt ttataccttt tccacgcaga ccaga
//
LOCUS       DAWDRC010000725         1664 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23723_length_1664_cov_1.630205, whole genome shotgun sequence.
ACCESSION   DAWDRC010000725 DAWDRC010000000
VERSION     DAWDRC010000725.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23723_length_1664_cov_1.630205"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    1205..1214
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          334 a          461 c          471 g          388 t
ORIGIN      
        1 taccagtttc cgcggggaga atggcaacgg catgttgacg caactcaacc gccagttccc
       61 gaccattagc ctgttagaca ttagcgcgat tttaaaacag gtcggtcagg tgctggagca
      121 ggtaagtcgg gcgctggaag tgatggtggt gctggtcacc gcctgcggca tgttgctgtt
      181 gctggcgcag gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac
      241 acttggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg ccatgctcgg
      301 gtttgtatcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcgg tactgcaatc
      361 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
      421 cggtgcgcta ctgctgtcgc tttgcggcgg ctggctgggt gcgcgactgc ttaagggtaa
      481 ggcgctgttc aggcagtttg cggggtgatg acaggtgtta gattgcctga tgcgctacgc
      541 ttatcaggcc tacgaagatc gtgcaatata ttgagttttc gaaattttgt aggccggata
      601 aggcgttcac gccgcatccg gcagttgtgc gccgatgcca gatgcgacgc aggcgcgtct
      661 tatcatgcct acgaaacagc actcacctgt aggctggata aggcgttcac gccgcatccg
      721 gcatgaacaa cgtgcacaga atcccccatc actgcgcctt aacccattcc gccacttccg
      781 cccactcacc gcgaaagaca actttttccg cttttttctc aagctgatag cgatacatcg
      841 ggtcgtaata ttcttcaagt aacggcacca gccaggccag atgaccgtcg gtgctgccgg
      901 tggtgagttg cgttgtcagc gccgcatcca gccttgcagc cagttcgtta tagcgttgta
      961 gtcccagccg acgtttaatc gccgaaagcc cgtgatgcag gtattcgcaa tacccctgcc
     1021 agccctgttc gtcgccataa gcgtgggtaa aatcatgatg catacgcaag aaatactctt
     1081 cgttcaggcg ttcaagacgg atctcaaacg gatcttctac caccgcaatc gtcgcctgag
     1141 tcattcgttc gcgcagacat tccggcaggt gattcgaacc gatcatccgg ctttcatctt
     1201 ccagnnnnnn nnnnccagcc cttccagatc aacaccgttc ggttgttgtt gcactaacag
     1261 cgttttgccg cagccggtac aaccgccaat cagcactatc ggtttttgtg acagttcaat
     1321 ggtcgcctga atcgcggtct ggcgcagtgc cttataaccg ccttccacca gcggataatc
     1381 aatccccgcg tcatgcaacc agcgttgcac aatatgtgag cgctgaccgc cacgggcgca
     1441 gcagagaata ccgtgcggat tttgcaggca cgctacccgc caggcgtcca tgcgctgctg
     1501 acgaatttca cccgccacca gtttatgccc cagcgccagc gccgcgtctg acccttgctg
     1561 tttatagcag atgccaacgg cggcgcgttc atcgttattc attaacggta ggttgatagc
     1621 ggcgggcatt gcgccttgct caaactcgat gggggcgcga acat
//
LOCUS       DAWDRC010000726         1660 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23788_length_1660_cov_1.895950, whole genome shotgun sequence.
ACCESSION   DAWDRC010000726 DAWDRC010000000
VERSION     DAWDRC010000726.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23788_length_1660_cov_1.895950"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          371 a          431 c          423 g          435 t
ORIGIN      
        1 taatggtcac cgcctcaaac accagcgttt ccagcggctg caacgtaatc accgtcgcgt
       61 ttttatactc aacgggcact gttttattgc tgccgtatac cgctttcaga ctaaactgcg
      121 ccgcgtttcc tgccggtatt tcgaaatcct tcgccaaatc cagatagtag gtctgtggct
      181 tatccgatgg gttgcgcaaa ccgagaatgg ctttgtcttt gctccaggat gcccagccgt
      241 acacggcaag cgccgttggg tcgccgccaa tccagtgggt atcaaccagc acgctggcat
      301 tttccttcga ccattttgca gccttcgcca gcgtatccca cttcaccttg ttcagcatgg
      361 acggggtaat atacagctcc tgcagctggg tgccggtcgc gaagtagctc cagacctgat
      421 cggcaaagtc gctgtccgtt tgcaccttct ctaacccgta ataggcattc tcggcgctga
      481 ctatcccgtg gtacatcagc gagttcagcg ggaacagcgg gcctttacgt acaatagagc
      541 ggtatgtctc ggcatcacga tatgttatcc actgctgcac cggcgtgccg gggccataca
      601 ggtttatatc atccccctga cgccagatag aatcagcata gaacaaccag gacgggctgg
      661 cgttggtgcc ggtggtcagg ttgataaata gattcgggtt tgctctgcgc atattgtgca
      721 gcagagctat tgacgcatca aaatccgagg cgaacgggct accctttata tgtgaactgg
      781 cgttccccat tccgtcgagt ttaaacgagg taatgtgttc atttttgata agattaatga
      841 tctgctcatt gaagttttta aagtagttcg ctcccgaaag cgccagcttg ccgtccacgg
      901 tttcgaaccc atactctttt gcatgcgaaa cgcgaacgtc gcgcggctta ttgtaacccc
      961 cccacggtga aagccatagc ccaacggagc tgtgcaggct atcggctttc tctcgtactt
     1021 tgctaaaacc gttgctgaat gccgggccaa ataaccagcg tccggtaaga tcgtcccagc
     1081 catcgtccag cagaaaagcg tctaacgcca ctccgcggcc gctaatgaat tccttgttcc
     1141 attcgtccat gcgtcccaga acatcctgtt cggtgtacgg agtgaaaaag ccgatatcca
     1201 tccaactgtt gtaatgcaaa taaggcttgt acggacgtgg acgtacggca ttaataaact
     1261 ggttcacact gcgtcgcaac tggctggttt ccgggaaagt accgaaccag gtggtaaagc
     1321 taaccggcgt ctctggctga attggagttt tcagttctac gttgagattg gttgttgctt
     1381 cataggcgta ggtattcacg atcggtttat tcggcaggat aaagaacgag tccgcaatga
     1441 tcggtgagct agtgattgcg ccgtctacgt aaggagcctg aaactgtttt ttggtcggga
     1501 agaaggtaat cttggcgacg tctcgtggtt gcccaacggc ggcaatagtg tagtcgatgc
     1561 tggcatattt tcccttcaca aggttcaact gcactgttac gttaaaatct gggcgcgtaa
     1621 agtcgatgac aatcgcattg tcctgttttt tgatgtgctt
//
LOCUS       DAWDRC010000727         1653 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_23867_length_1653_cov_2.354819, whole genome shotgun sequence.
ACCESSION   DAWDRC010000727 DAWDRC010000000
VERSION     DAWDRC010000727.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23867_length_1653_cov_2.354819"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          354 a          468 c          471 g          360 t
ORIGIN      
        1 tctctttaaa cagcggcagg tcagcagggg taatcccgcc agtaatggaa agctcaaggc
       61 cgatatcgga aagcgctttc atgcgtgcca gatcggcctc gccccactgt tgcccgctgg
      121 cctgcgcgtc acgaccacga tgataaatcg cctgtcgtac accaatacga tgccagtcac
      181 gggcatcgtc cagcgtccag ttaccgaata gctcaatctg aatttcacca ccgcagttct
      241 gtgccatcgc gtggcctttt tccaccgtgg cgagcggcgc ggcgcagatg atggtcatcc
      301 agttagcgcc tgcgccaaat gcctgttgcg cgagcgtttc acccgcgtcg gcgaccttcc
      361 agtcggcaac gatgattttg tcagggcact gttcgcgcaa ggctttcact gcgccaagcc
      421 cttcgtttaa acagagaatg gtgcctgctt cgacaatatc gacgctgtct tttaacagcg
      481 tcacgtcgcg ctgcgcggct tcaagtgatg agtggtcgag ggccagttgc agaagtggtc
      541 gactcataat gtgtgctcct tgatgcgggc gtgataaccc tgaagtgcgg caatgagatg
      601 ttgatagcgt tggtattttt gttggtaaag ctgatgcgcg gccatattcg gcagcagggt
      661 gcgtaccggg tgctgtaagt cacgttgggc ttcgctgaaa tcgcgataaa ccccggtacc
      721 gacgcgggcg gcaagtgccg cgccaaaaca gccggtttct tccacctgcg gcagctcgat
      781 acgcagaccg ctgacgtccg ccagcatttg catccagaca tcggagtgcg ccgggccgcc
      841 agtgacgcgc agggtgtgca catcagtaaa acgttcgcgc atccggttga gatgggtcat
      901 atggctgaac accacccctt catagatggc ctgtaagagg tgtgcacggg tgtgaatggc
      961 ctgcatcccg tagaaaccac tggtcatttc gagtccggcg ttgctgccgt acaggaacgg
     1021 caggaaaaag agatcgccac cggctttcgg caaactggca acggcctgat taatctcagc
     1081 aaatgagatt tctccccact gtgcggtaaa ccattcgaga ttgccggaag aggtggggct
     1141 ggcttcgtga acgataaatt gaccatcgtt aacgtagcga ccatagacat acggatgcgc
     1201 ttcaccgtca cgtaaaccgt gggttatgcc gctggtcacc gcccaggtcc ccatcaccgc
     1261 attgagggtg aattcgtctt cgagtccggc gcggagtgcg gtggaaacca catcaaacag
     1321 gccgccgaca acgggggtac ccgctttcag accggtcagt acggctgtct gagcggtgat
     1381 ttccccgcag atttcggcag atccgacaac aggcggcagg gcgtgattga tttcagcaat
     1441 ccccagccag tcggtaagac acgggtcata ttccccgcga ctcatgttgt agagattgga
     1501 ctcagaaata ttgctctctt cgcaaccttt gacgccggtt aaacaccagc gcaggtagtc
     1561 gtgcgtcatc atcacgcagc caatttgcgc gtagcgttct ggttcgtgct ctttcagcca
     1621 gcgtaacagc gacaccggat gcccggtcca taa
//
LOCUS       DAWDRC010000728         1643 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24047_length_1643_cov_2.231108, whole genome shotgun sequence.
ACCESSION   DAWDRC010000728 DAWDRC010000000
VERSION     DAWDRC010000728.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24047_length_1643_cov_2.231108"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          382 a          344 c          463 g          454 t
ORIGIN      
        1 atcgcttggc tggtgcgccc ggaattgttc actctcaaac cgtgttttgt cgcggtagaa
       61 actcagggtg aatttacctc aggcacgacg gttgttgata tcgacggttg cctgggcaaa
      121 ccagccaatg tacaggtggc gctggatctg gatgtgaaag gcttccagca gtgggtggct
      181 gaggtgctgg ccctggcgct ctaataataa aacaaggaag tgttctatga cgtatggaga
      241 agcgtatctg gaagggtgga aaaacatctt taattatgaa ggtgtttcca atcgcttcga
      301 gttctggtcg tttatgattg gtagtggcgt tatctgtctg cttccgcttt tatgttggtg
      361 gttagcggta acaattaaca atgactatgg tgttttcatt ttttttgcgc tgcccgtgtc
      421 atttatttta acgttgattt ttgccgtacc ggcgatagcg ttagccgtca gaaggatgca
      481 cgatatcgga tattcagggt ggtgggttac cattgccgtg ttgatccctg ttacgggcgt
      541 aattctgctt atactgtgct gcctgccgtc aaagtcacaa gaccaggcat aatgccagag
      601 ggtatgacgc atcactttct gcgtcatgcc tttcactgat atccctccct gtttatcatt
      661 aatttctaat tatcagcgtt tttggctggc ggcgtagcga tgcgctggtt actctgaaaa
      721 cggtctatgc aaattaacaa aagagaatag ctatgcatga tgcaaacatc cgcgttgcca
      781 tcgcgggagc cgggggccgt atgggccgcc agttgattca ggcggcgctg gcattagaag
      841 gcgtgcagct gggcgcggcg ctggagcgcg aaggatcttc tttactgggc agcgatgcgg
      901 gcgaactggc cggagccggg aaaacaggcg ttactgtgca aagtagtctt gatgcgataa
      961 aagatgattt tgatgtgttt atcgatttta cccgtccgga aggtacgctg aaccatctcg
     1021 ctttttgtcg ccagcatggc aaagggatgg tgatcggcac tacggggttt gacgaagccg
     1081 gtaaacaggc gattcgtgac gccgccgccg atattgcgat tgtctttgcc gccaatttta
     1141 gcgttggcgt taacgtcatg cttaaactgc tggagaaagc agccaaagtg atgggggact
     1201 acaccgatat cgaaattatt gaagcacatc atagacataa agttgatgcg ccgtcaggca
     1261 ccgcactggc aatgggagag gcgatcgccc acgctcttga taaagatctg aaagattgcg
     1321 cggtctacag tcgtgaaggc cataccggtg aacgtgtgcc tggcaccatt ggttttgcca
     1381 ccgtgcgtgc aggtgacatc gttggcgaac ataccgcgat gtttgccgat attggcgagc
     1441 gtctggagat cacccataag gcatccagcc gcatgacatt tgctaacggt gcggtaagat
     1501 cggctttgtg gttgagtggt aaggaaagtg gtctttttga tatgcgagat gtgcttgatc
     1561 tcaataatct gtaaccataa gatatttatg gtgatgcaaa aataacacat ttaattcatt
     1621 gaatataaag ggctttaatt ttt
//
LOCUS       DAWDRC010000729         1643 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24049_length_1643_cov_2.015743, whole genome shotgun sequence.
ACCESSION   DAWDRC010000729 DAWDRC010000000
VERSION     DAWDRC010000729.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24049_length_1643_cov_2.015743"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          396 a          414 c          416 g          417 t
ORIGIN      
        1 gcgtggagct gcggtttgtt ggcgacgaat gtcaggttgc gggcgatccg ctgatgctgc
       61 gtcgggcgtt aagcaatctg ctttctaatg ccctgcgtta tacgccaacc gaagagacaa
      121 ttgttgtgcg ctgccagacg gtcgatcacc tggtgcaagt taccgtcgaa aaccccggta
      181 cgcccattgc gcccgaacac ttaccgcgat tgtttgaccg tttctaccgc gttgatcctg
      241 cccgccagcg aaaaggcgaa ggtagcggca ttggcctggc gatagtgaaa tcgattgttg
      301 tcgctcataa aggcacagtt gcagtaacgt cagatgtgcg ggggacaaag tttgttatca
      361 tattacccgc ttaatccttc agcaaacggc aacttttata accagtgtaa aaataacatg
      421 ccgcaatagt aatacctatt aatgataaaa agctgtcaca attcataaaa aaccttaata
      481 tacgccaccc taaacataac cagcgttaat gtaaggtttt tgtgtggact ggctccttga
      541 tgtttttgct acctggctct acggcttaaa agtaatcgcg ataacgttag cggtcattat
      601 gttcatcagc gggctggacg atttttttat tgatgtcgtc tactgggtac gccgcattaa
      661 acgcaagttg agtgtttatc gccgctaccc gcgaatgagt taccgcgaac tgtataaacc
      721 agatgaaaaa ccgttagcga ttatggttcc ggcgtggaat gaaacgggcg tcatcggcaa
      781 tatggccgag ctggcggcga ccacgctcga ctacgaaaac tatcatatct ttgttggcac
      841 ctaccccaac gaccccgata ctcagcgtga tgttgacgaa gtgtgcgctc gcttcccgaa
      901 cgtgcataag gtagtctgcg cgcgtcctgg ccccaccagc aaagccgact gtctgaacaa
      961 cgtgctggat gccatcaccc aatttgaacg tagcgccaat ttcgcttttg ctggttttat
     1021 tctgcatgac gccgaagatg tgatttcacc gatggaattg cgtctgttca actatctggt
     1081 cgagcgtaaa gatctgattc agatcccagt gtatccgttc gaacgcgaat ggacgcactt
     1141 caccagcatg acctacattg atgagttttc agaactgcat ggcaaagatg ttccggtgcg
     1201 tgaagccctc gctggacagg tgcccagcgc aggcgtcggt acctgtttca gccgccgcgc
     1261 cgtgaccgca ctgttagctg acggtgacgg tattgctttc gacgtgcaga gtctgactga
     1321 agattacgac attggcttcc gcctgaaaga gaaaggtatg acggaaattt ttgtccgttt
     1381 tccggtggtg gacgaagcca aagaacgcga gcagcgtaaa tttttacagc atgcgcggac
     1441 gtcaaacatg atctgcgtgc gcgaatattt ccccgatacc ttttcgactg cggttcggca
     1501 aaaatctcgc tggatcatcg gcattgtttt ccagggcttt aaaacccaca aatggacctc
     1561 cagcctgacg ctgaactact ttctctggcg cgaccgcaaa ggggcaatca gtaactttgt
     1621 cagcttcctc gccatgctgg tga
//
LOCUS       DAWDRC010000730         1637 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24124_length_1637_cov_1.870417, whole genome shotgun sequence.
ACCESSION   DAWDRC010000730 DAWDRC010000000
VERSION     DAWDRC010000730.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24124_length_1637_cov_1.870417"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          396 a          403 c          497 g          341 t
ORIGIN      
        1 ggttttggcc gttttggcct tttacaactg acttcaggcg gtctgttctt taatgcctta
       61 aagaacgaca aagaaaattt cactgtttta caaaccattc gccagcagca atccacgctg
      121 aatggcagct gggtcgcgtt gttgcagacg cgtaacaccc tcaaccgcgc gggtatccgc
      181 tacatgatgg atcagaacaa tattggtagc ggttcaaccg ttgctgagct gatgcagagt
      241 gccagtattt cgctgaaaca ggcggaaaaa aactgggcgg attacgaagc gttgccgcgt
      301 gacccgcgtc agagcaccgc cgcagcggca gagatcaaac gtaattacga tatttatcac
      361 aatgcgctgg cggagctgat ccaactgtta ggtgcaggca aaatcaacga gttctttgat
      421 caaccgaccc agggatatca ggacggtttc gagaagcagt atgtggctta catggagcaa
      481 aacgatcggc tctatgatat cgccgtcagc gataacaatg cctcctacag ccaggcgatg
      541 tggattctgg ttggcgtgat gatcgtcgta ctggcggtca tcctcgccgt ctggttcggt
      601 attaaagcct cgctggtcgc gccaatgaat cgcctgattg acagcattcg tcatattgca
      661 ggcggcgatc tggtgaaacc gattgaggtg gatggctcta atgagatggg gcaactggca
      721 gagagtttgc gccatatgca gggagagctg atgcgtaccg tcggtgatgt gcgcaacggg
      781 gccaatgcca tctatagcgg tgccagcgaa atcgccaccg gcaataacga tctctcttcg
      841 cgcaccgagc aacaggccgc ttcgctggaa gagacggcag ccagcatgga gcaactgacc
      901 gcaacggtga aacagaatgc cgaaaatgcg cgccaggcca gccatctggc gttaagtgct
      961 tccgaaacgg cgcaacgcgg cggcaaagtg gtggataacg tggtgcagac catgcgcgat
     1021 atctccacca gttcgcagaa aatcgccgat attatcagcg taattgacgg cattgccttc
     1081 cagaccaata ttctggcttt gaacgcggcg gttgaggctg cgcgtgcggg tgagcaagga
     1141 cgcggttttg cggtggtcgc gggagaagtg cgtaatctgg cccagcgtag cgctcaggct
     1201 gctcgtgaaa ttaaaagcct gattgaagac tcggtgggga aagtggatgt tggctctacg
     1261 ctggtcgaac gcgccgggga aacaatggcg gagattgtca gtgctgtgac ccgcgtgacg
     1321 gacattatgg gcgaaattgc ttctgcttct gatgagcaga gccgtggtat cgatcaggtt
     1381 ggcttagcgg ttgctgagat ggaccgggta acccaacaaa acgccgcgct ggtggaagaa
     1441 tctgccgctg ccgccgccgc gctggaagag caggccagtc gcctgaccga agcagtggca
     1501 gtgttccgga ttcagcaaca gcagcaacag cagcaacagc agcgtgaaac atcggctgtg
     1561 gtaaaaaccg tgatgccagc tgcgccgcgt aaaatggccg tggcagatag cggtgagaac
     1621 tgggaaacgt tttaatc
//
LOCUS       DAWDRC010000731         1625 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24323_length_1625_cov_2.477070, whole genome shotgun sequence.
ACCESSION   DAWDRC010000731 DAWDRC010000000
VERSION     DAWDRC010000731.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24323_length_1625_cov_2.477070"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          364 a          472 c          433 g          356 t
ORIGIN      
        1 atttccggca gctgcggctc ggcttcaaac gctttcgcca gcgtcatccc cggatcgggc
       61 gggatcagtt tcgagatacg gtcgacaaag ccgtacggat gccccagcac gcggcctacg
      121 tcgcggatca ccgctttcgc cgccatcgta ccgaaggtga taatctgcga taccgcatca
      181 cgaccgtaca tgtccgctac gtgctcgata acctggtcgc gtttctccat acagaagtca
      241 acgtcgaagt caggcatgga gacacgttcc gggttaagga aacgttcgaa cagcaggtca
      301 aattccagcg gatcgagatc ggtgattttt aacgcgtagg ccaccagtga ccctgcacca
      361 gaaccacggc ctggccctac cggtacaccg ttatctttcg accactggat aaattccata
      421 acgatgagga agtaacccgg gaagcccatc tggttgataa cctgaagttc agtatccaga
      481 cgttcgtcat attccgggcg gcgcttaaga cgtccttcct cgtcagggaa taaaaaggcc
      541 agacgctctt ccaggccctc ttttgcacgc ttgaccagat aatcttcggt gctcatgtcc
      601 ccggtcggga actgcggcag gaagtattca ccaaggcgca cggtcacgtt acagcgtttg
      661 gcgatctcaa cggtgttggc aagggcttcg gggatgtcgg caaacagctc acacatctcc
      721 tcttcgctac gcatatattg ctgcggcgaa tagttacgag ggcgtttagg atcgtcgagg
      781 gtaaagccgt cgtggatcgc gacgcggatt tcgtgtgcgt caaagtcgct gctgtcgata
      841 aagcgcacat cgttagtcgc cacgacgggc aaaccgcgcg cttccgccag ttccaccgcc
      901 gcgtgcagat agctttcttc atccggcctg ccggtgcgga tcagctcgag aaaatagcga
      961 tccgggaagt gttcttcata aaacgcgaca cactcatcta ccagcgcgct gttaccacgc
     1021 aaaagactgc gtccaacgtc gcccatgcgc ccgccggaaa gaaggatcaa cccttcattt
     1081 aattcgataa gccagtcgcg atcgatgatc ggcccggcgg taccgtaccc gcgctgatac
     1141 gcttttgaga tcagcaacgt cagattctga tagccggtat tgttcgccgc cagcaccgtc
     1201 aggtgagtta actcatcacc cagcaggtcg cactggacgt taaaatccgc cccgacgatg
     1261 ggcttaatcc ctgcgccatg tcccgctccg tagaacttca ccagaccgca aaggttggtg
     1321 aaatcggtga tcgccagtgc tggcataccc aacgctgccg ccttttttac cagcggtgcg
     1381 gttttggcca ggccatcgat catcgagtag tcactgtgca cccgcaggtg tacgaaacgt
     1441 ggttgagaca tcttcagatt ccggtttact taatctcgac acaagaatca ggacgcaagt
     1501 cccagtgcgc gtttgacagg cccaaagctg cgccgatggt gttcggtcgc gccgtgttca
     1561 gccagttttt ccagatgaaa agcggttgga taccctttgt gttgggcaaa accatattgc
     1621 gggaa
//
LOCUS       DAWDRC010000732         1624 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24351_length_1624_cov_1.941364, whole genome shotgun sequence.
ACCESSION   DAWDRC010000732 DAWDRC010000000
VERSION     DAWDRC010000732.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24351_length_1624_cov_1.941364"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          461 a          429 c          357 g          377 t
ORIGIN      
        1 ccttgcggtt gattttgata gacataatcc agcgaactgc ctgaatcaaa gccggtactc
       61 attccgagat gcaggcccgc agagtgcgcc ccgaacagtt tcatcgcctt tcgcataaag
      121 cgatacgcca aatcaaccgg cgataacggc acgggtccac cagaaaggag ccgccatcga
      181 tccgctgatg gtcctgtgcg gtcttcatgc tgataatcaa atttttgcga cttgttagca
      241 tgtaaacggc tgataaagct ttgcattttt tcaaatgcct gcgccctgtt ctcttcaccc
      301 aacgtgtcgt gataaaagcc tggcagcaga tgcagctctt tcagaggact acgtaaacgc
      361 tgataaaaat caatctgcgg ttggcgatgc accacatagt catcgcctga tatcagaagt
      421 tgcgtgggga gcgtaatcgc cgccgcatcg ctaacaatac gttcagacgt tttatagaga
      481 tcgagcaaga tattgacggc aatcgcccgt gtgatcagcg gatcattatt gaaactcgcc
      541 acccgttgcc aatcgtgggt cagataacgc cctttcacat aggaattaat aaaaaacaga
      601 ccacgcagac gatgccataa cgccagccca ggacgtgcca gcggcacata caatttaacc
      661 ttaaaggccg gagatgccag cactagtccg cgaatagcag gcgcataatc atgaacccat
      721 gtggcaacca tcactgcgcc gacgctttgc gcgatcacaa ccacctcttc cagtccgacc
      781 tggctgtcgc tggcagcaaa acggacaaat tcatcaacat cctgcactga acgcgcaaga
      841 gacggactat aaccacgcgg ccccgaagtt tgtccatgcc ctcgggcatc ccatgcataa
      901 aaagcagtat ctggcatcgc cagttcatca acgatatgtt gtagacgacc agaatgttca
      961 tgaccgcgat gaaataagac gatgaccttt ttcgcccctg gctgtaaagt gggccagtgc
     1021 cgataaaaca acgctgtatt atcactggtg gtaaaaaaat gttccccagg gatgcgtgaa
     1081 ttttccattt tctctatttt ccttcagtaa cagcagtagc gaataaaata aaaaaatacc
     1141 ggagcggtga aaattaatga gtcaatcctg tcaaggagtc cgccatgtcc tggtaatagt
     1201 tttccactat ctttaacacc aatatctcgt ttgatggctg acatcactac atcgccgcaa
     1261 aaaccactaa taccaattaa caaacccgct aataatgcct gtaatgtatc tagcggtgtc
     1321 agcaacggtc caataatcag tgacgcaatc atggtggtga ttacgccgcc catcagacct
     1381 tccaatgttt ttcccgggct gactttaggg accacttttc ttctgccgca ggattttccc
     1441 cataaatact gtgcaatatc gtttgactcg gttaatgcca aaagaaataa caccagtaac
     1501 gccccgccct ggatatttgt ggttggcaac attaataacc aggcggcatg actcaaggcg
     1561 aaaacggtca ttatccagcc acaaaaaatg gcgctcacgg tatgcagaaa accggagggg
     1621 tcac
//
LOCUS       DAWDRC010000733         1609 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24567_length_1609_cov_2.696268, whole genome shotgun sequence.
ACCESSION   DAWDRC010000733 DAWDRC010000000
VERSION     DAWDRC010000733.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24567_length_1609_cov_2.696268"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    869..878
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          339 a          405 c          461 g          394 t
ORIGIN      
        1 caaggtggcg tatgaaacca cctcttttgt aaaagcctcc attgaagacg tggtgaaaac
       61 gcttctggaa gctatcgccc tggttttcct cgttatgtat ctgttcctgc aaaacttccg
      121 cgccacgctg atacccacta tcgccgtgcc agtggtgttg atgggaacct tctccgtgct
      181 ttacgccttc ggttacagtg tcaacacctt aaccatgttc gcgatggtgc tggcgatcgg
      241 tctgctggtg gatgatgcca tcgtggtggt ggaaaacgtc gaacgtatta tgagtgagga
      301 aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg gggcactggt
      361 cgggattgcg atggtgcttt cggcggtatt tgtgccaatg gccttcttcg gcggcaccac
      421 cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc tgtcagtact
      481 ggtagcgatg atcctcactc ctgctctgtg tgccacgcta cttaagccac tgaaaaaagg
      541 tgagcatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta accgcaacgc
      601 cgaacgctac gaaaaagggg tggcgaaaat tctccaccgt agcctgcgct ggattgtgat
      661 ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga cgtcgttctt
      721 accgctggaa gaccgtggca tgtttactac ctcggtacag ttgcccagcg gttcaaccca
      781 gcaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc atgaaaaaga
      841 caacatcatg tcggtgtttg ccaccgtgnn nnnnnnnnca acgaaggcgt ttaacaagat
      901 taaagaagct cgcgttatcg ccagcagccc gccagcaatt agcggtcttg gtagttctgc
      961 gggttttgat atggagttgc aggaccacgc tggagcgggt cacgatgcgc tgatggcagc
     1021 acgaaaccag ttgctggcgc tggcagcgga aaacccggag ctaacccgtg tgcgccataa
     1081 cggcctcgac gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct
     1141 gggcgttgct atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt
     1201 gaatgacttt atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta
     1261 tcgcatgctg ccagatgaca tcaatctctg gtatgtccga aataaagatg gcggcatggt
     1321 gcccttctct gctttcgcga cctcacgctg ggagacaggc tcgccgcgtc tggaacgcta
     1381 taacggttat tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac
     1441 ggcgatggat attatggaat cgttagtgaa gcagttgcca aacggctttg gtctggagtg
     1501 gacggcgatg tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgccat
     1561 ttccttgctg gtggtattcc tgtgtctggc tgcgctgtac gaaagctgg
//
LOCUS       DAWDRC010000734         1603 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24681_length_1603_cov_2.167959, whole genome shotgun sequence.
ACCESSION   DAWDRC010000734 DAWDRC010000000
VERSION     DAWDRC010000734.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24681_length_1603_cov_2.167959"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          350 a          381 c          473 g          399 t
ORIGIN      
        1 attgtctgcg gtttgatgat gtcggcatta ggcacgttgc tggtcgtgca atcttcctcg
       61 ccagcccagg cggtagcgtt tatctcaatg gcgctgttct gcgtgcattt cgcgggaaca
      121 tctgcatggg ggctggtgca ggtgatggtg tcagaaacaa aagtggcttc catcgccggg
      181 attcaaaact ttggcagttt tgtttttgct tcctttgctc cgatcgtaac cggttgggtc
      241 gtggatacca cacactcgtt taatctggcg ctggttatcg cggcctgtgt gacgttcacc
      301 ggggcgctgt gttacttctt tattgtcaaa gatcggattg agtaaccgtt actatcaggc
      361 tctcttgctg tgggcctgta gaataaaaaa aagcggatcc tggagatccg caaaagttca
      421 cgttggcttt agttattcga gttgagaaac tctcgaaacg ggcagtgact tcaagggtta
      481 aaagaggtgc cgctccgttt ctgtgagcaa ttatcagtca gaatgcttga tagggataat
      541 cgttcgttgc tatgctacct atcgccgcga actatcgtgg caatggagga tggataatga
      601 atattcgtga tcttgagtac ctggtggcat tggccgaaca ccgccatttt cggcgtgcgg
      661 cagattcctg ccacgttagc cagccgacgc ttagcgggca aattcgtaag ctggaagatg
      721 agttgggcgt gatgttgctg gagcggacca gccgtaaagt gttgttcacc caggcgggaa
      781 tgctgctggt ggatcaggcg cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg
      841 caagccagca gggcgagacg atgtccggac cgctgcatat tggtctgatt cccacggttg
      901 gaccgtacct gctaccgcat attatcccga tgctgcacca gacctttcca aagctggaaa
      961 tgtatctgca tgaagcacag acccaccagt tactggcgca actggacagc ggcaaactcg
     1021 attgcgtgat cctcgcgctg gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg
     1081 atgagccaat gttgctggct atctatgaag accacccgtg ggcgaaccgc gaatgcgtac
     1141 cgatggccga tctggcaggg gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg
     1201 atcaggcaat gggtttctgt tttgaagccg gggcggatga agatacacac ttccgcgcga
     1261 ccagcctgga aactctgcgc aacatggtgg cggcaggtag cgggatcact ttactgcctg
     1321 cgctggctgt gccgccggaa cgcaaacgcg atggggttgt ttatctgccg tgcattaagc
     1381 cggaaccacg ccgcactatt ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct
     1441 atgagcagat ggcagaggcc atccgcgcaa gaatggatgg ccatttcgat aaagtgttaa
     1501 aacaggcggt ttaagccatt tagcgcagct acccgatagg cttccgccat cgtcgggtag
     1561 ttaaaggtgg tgttgacgaa gtactcaata gtgttgccgc cac
//
LOCUS       DAWDRC010000735         1601 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24712_length_1601_cov_2.038163, whole genome shotgun sequence.
ACCESSION   DAWDRC010000735 DAWDRC010000000
VERSION     DAWDRC010000735.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24712_length_1601_cov_2.038163"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          357 a          421 c          420 g          403 t
ORIGIN      
        1 tggtccaacg cggtggaatt tctgcctgaa attaaaccgt atcgtctgga tttattgcat
       61 agcgtaaccg ccgaaagcga agagccactg agcgccgggc aaattaagcg cattgaaacg
      121 ctgatgcgca acctgatgcc gtggcgcaaa gggccgttct cactgtatgg cgtcaacatc
      181 gataccgaat ggcgttccga ctggaaatgg gatcgcgtta tgccccatct ttctgattta
      241 accgggcgca ccattcttga tgttggctgc gggagcggtt atcacatgtg gcgcatgatt
      301 ggcgcaggcg cgcacctcgc ggtggggatc gaccctacgc aactgtttct gtgccagttt
      361 gaagcggtgc gtaaactact gggtaacgat cagcgcgcac atttgttacc gttaggtatt
      421 gaacaacttc cggcactgaa agcctttgat accgtctttt cgatgggcgt gctttatcat
      481 cgtcgttcac cgctggagca tctctggcag ttaaaagacc aactggtgaa tgaaggcgaa
      541 ctggtgctgg aaacgctggt tattgatggc gacgaaaaca cggtgctggt gccgggcgat
      601 cgttacgctc aaatgcgtaa tgtctatttc attccttccg cgttggcgct gaaaaactgg
      661 ctgaagaagt gtggttttgt tgatattcgc attgcagatg tgagcgttac caccacagaa
      721 gagcagcgac gcaccgaatg gatggtcacc gagtctctgg ccgattttct cgacccgcat
      781 gatccgggta aaacggtgga aggttatcct gcgcctaaac gcgcggtgct gattgcgcgc
      841 aagccgtaaa ggtctggtaa tactgccgga tgcggcgtga gcgccttatc cggcctacaa
      901 aaccttgcta attcaatata ttgcagggac tatgtaggcc tgataagcat agcgcatcag
      961 gcagttttac gtttgcataa cctcagcgcc cgtttccggg cgcttttcac gtcttatgcc
     1021 tgaacagctg gctgatcaaa cgccgttaac ttcagcgcat tgcccgtata tttttcgata
     1081 tacaccagcg cagagttacc ggcacaggca ttcgccagct gcgagctggg gatatccgcc
     1141 gttaacacgt tcgcactacc gtttttacac aagccatttt ccagatctgg ccatgcacct
     1201 tcatgcacgc ataccacgcc ttttttgatc ccgtcagtca ccaccgcgcc tgtcagaatc
     1261 tgaccgcgct tgttccagac acgcaccaga tcgccattcg caataccaaa gcgagcagca
     1321 tcttcggtgt gaatagtgat tggttcacga tcggcaaccg catatttttt acgcagttcc
     1381 gcatagttaa gctgactgtg taaacggtgt gccggatgtg cggtcagaag ctgcaactgc
     1441 ttctcgtcgg cggtaccctt ccactcatca ggcgcaagcc aggttgggtg tgccgggcaa
     1501 tccttatagc caaatttttc cagcgttctg gagtaaatct caattttgcc gcttggtgta
     1561 cccagcgcat ttttcaccgg atcggcgcgg aaatcaccat a
//
LOCUS       DAWDRC010000736         1591 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24885_length_1591_cov_2.049479, whole genome shotgun sequence.
ACCESSION   DAWDRC010000736 DAWDRC010000000
VERSION     DAWDRC010000736.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24885_length_1591_cov_2.049479"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          376 a          465 c          424 g          326 t
ORIGIN      
        1 aacaccgggc tttgccagct aatttcgcgt ttgatgaatt cgtagcgcgg cacctgcgac
       61 accgccgcac ggttaaccag ctccaggtgg gagaagatca cctgggtggt ggtggtgccg
      121 atatcgatac cgacgctcag tagctggcga gtgttcacga ttgcgcctcc gcttcagttt
      181 tagccgcggt cgcgtcttct tttggcacca gcatcatcgc cacgccaatt gccgttacgc
      241 cgccgatcaa tttgccgaca atcatcggga agatcatggc gttcatgttg gcagcggcga
      301 agcctaaatg gtcgcccagg gcgaaagcag cggaaacggc gaaggcgcag ttgatgactt
      361 tgccgcgggt atccatctgc ttcatcatgc caaacatcgg aatgttgttg gcaagcgttg
      421 ccaccatgcc ggctgccgcg atattgttca tattcagcac cttaccgacg ctcatcagcg
      481 gtttttcaaa ccagcgagtc agcagcagta ccattggata cgcccctaac agaacgcagg
      541 agatcgagcc gataacttca atggcgcgca tcacttcacc gggtttatcg ccaggggcca
      601 taaagatagg atcaagaccc gggatcagtt cccagccaag gaggaatttc actaccgcag
      661 cggcaagacc gagggtgatc aatgcaacga ggaatttggc gaagatctgg aagccgttga
      721 tcattttttc cgggatgaat ttcagcccca gcgccaccag caccgcaacg ataagtaccg
      781 ggatcatgtt catcaggatc agcgcaaagg tgaattcaac aggctggccg ttgatctgca
      841 caccggagta catcgctacc agaccaccag cgatacagcc aatcggaatg gtcactatgc
      901 ccgccagcac gccgagcgcc agataacgac ggtcagaagg ttcgataatg ccgagcgcca
      961 ctggaatgga aaacacaatc gttggcccca tcatcgaccc gaggattaac ccagagtata
     1021 gccacgctgc tacgtcaccg cccgccagct ctttggcgag gaagaagccg cccatatcgc
     1081 acgccagcag tgttccggcg aacattgacg ggtttgcgcc gagcatttcg taaaccggga
     1141 taatcaccgg cccgagtacg tgggccagta ccggtgccag tgcggtcata ccgaccatag
     1201 ccaggcccag agcgcccatt gccataaagc cttcttcgaa ctgaccgccg gatccttcga
     1261 tacttttacc gaacttaccg aggaaacgag cagaaccgcc gaactgcgac aggatcctgt
     1321 ctacggcagc tatcagcata aagaacatca tgatgtacat gatgatttcg ttaattccca
     1381 tcgcctttac tccctgttag ttgttattta ttggcggatg cggcgtgaac gccttatccg
     1441 ccctacatgt gcaatcccgt aggccggata agacgcggca agcgtcgcat ccggcattta
     1501 ttgcgccgct gcgtacaggc cgacaatctg ctccaggctg gcggtacgcg ggttgctgcg
     1561 cagacaaata tcttcctgcg cggcctgcgc c
//
LOCUS       DAWDRC010000737         1588 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_24931_length_1588_cov_2.166993, whole genome shotgun sequence.
ACCESSION   DAWDRC010000737 DAWDRC010000000
VERSION     DAWDRC010000737.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24931_length_1588_cov_2.166993"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          384 a          469 c          363 g          372 t
ORIGIN      
        1 acgggtgatc cacaacgtgg gccaggcgtc tggcccatgc cagttatcgc aggtgggttc
       61 tgcggcgtaa cgcaccaggc gaaaacgctg accgtcaaag cgccagcgcg cctgaatgcc
      121 acaatcgctt aatccgcgcc cttttgctaa ggtcaccagt tcacgcgatt tttcatcgaa
      181 tgttgcgttc atcagttcca gttcattggt ctcctgaccg ttgttgaacg gcaaacgcaa
      241 ccgtaccggg cgcgaggcca gcggcttttt acgcgacaca atccatgcca aatcaatggt
      301 gttataggcc cctgcctcac agctaatcat catcagcgct ttatcatcgg tcagcgcagt
      361 gacattcacc tcacgacgta acggatcaag cgaacagcgc agaccattca tccgccagtt
      421 tccataatcc agcaaatcgt tacgttcttc gagtgagagt ggcgtcggcg ttgggttaac
      481 caccgcgacc tctttcagcg caggcgctgg cggtacgctg agcggcggtt cgtccccttt
      541 ctttatccac gcggtctcac tgccaacgcg cttttgctgg gcatcaataa acaacaacgc
      601 cgcttttaag ccactcagag aaatagtctg attgccgtcg cgtaaggtga tcgccctccc
      661 ttcctgaatc atctgcaaaa acgcggtgat ggttgccgta tcatcggtta ctaataacca
      721 tggtgaaatc cgccacttgt caccacttaa cgccaacggc tcaccatcta acagcagccg
      781 tggcgctatc tccccttctg acgcatccgg cgacttcaac ccgccgcgct caatacgtaa
      841 aacggcatcg gtatgcgccc cggcgctgcg gctcagggtc atcaccagtc cattatgatc
      901 gcccgtattg cgcgctacac agaaattttg attattacag gtgacctgcc agtcggaaaa
      961 cgcccgttgc gctggtgccg cccacaccaa agacatcggc aacaaagcga aaaaaaagag
     1021 aaggaaaatg cgatatcgca tggacggtac gaccccagaa gttcaaacaa acagcacaag
     1081 tcgtatcttc gttgcgcagg tagcgcggct caatcggatt tgtcggattg agccaataat
     1141 aaacaacgaa atattattgt tatttttcag tccattaaat gtgacatttg caaatagtta
     1201 agcaataaca ccgtcttacc gtcacgtatc tcgccggttt taatcatctc cagcgcctgg
     1261 ctgaacggca gctcaagcac ttcaatatct tcatcttcga caccaccacc ggcgttagcg
     1321 cgctggctgt cactgtattc ggcgataaaa aagtggatta gctcagtcac accgcctggc
     1381 gacatataca gttcaaataa tttgcggact tcgccaactt catagcccgt ctctccaatc
     1441 gcttccttgc gaatgcacac ttccggttcg tcgttatcca gcagcccggc acaggtttca
     1501 atcagttgcc cgctttcatt gccattaacc caggtagcga cgcggaactg gcgaatcaga
     1561 accacggtct ttttcttcgc gttgtaca
//
LOCUS       DAWDRC010000738         1579 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_25091_length_1579_cov_2.019029, whole genome shotgun sequence.
ACCESSION   DAWDRC010000738 DAWDRC010000000
VERSION     DAWDRC010000738.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25091_length_1579_cov_2.019029"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    770..779
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          330 a          433 c          410 g          396 t
ORIGIN      
        1 cacggttccg ccagttcaat ttgcagccac actgccagcg caatggtgct gtcgggaacg
       61 gcgcgaaaag ccaggcgcgg atcgcttttg gcaaccattt tttccgccga caggtgagtc
      121 agcgtatccg gcttttttgc caccggctgg cggcacagtt cgcagcacgc ctgcggatgt
      181 tcatcgctac tggtcagccc gtgaacgggc tgcggctgct gctgttcatc gtcaacaaac
      241 aggcggccct gctcgtcaat aaacttgatg cgcagatggc ggctttccag taactcccgg
      301 gcagagggcg tcaggcgact gtccgcaggg agatggatct ctgcgccttc gctgagcgta
      361 tggttcgctc ttagccatgc ttcggtgatg aaatctttca tagggattgc cagttagccg
      421 gccaggcgac atacaggaat ttcacgctgg atgtcgtacc aaattcgatg ctggagcctt
      481 tcgggataaa catcacatcg cccgctttgg cgatcatagt ttcgccttcg tggcgaacat
      541 gcagctcccc ttccagcacc atgtcgattt cgtcgtagtt aagggtccac ggaaagaagg
      601 cgttctccca ctgcatgaaa ccagcggcca tactgctgcc gtcgtctccg gtcacgagat
      661 cagtcaagcc tacgcagtgc ggttgtgcgc cgtcgaaacg accaaacttg acgctgctgc
      721 catcaataac tttgatgccg ccttggccgg ccaccgattc aaagctcggn nnnnnnnnna
      781 ggctttcggt aaactgacct tccggcagtt gggcgataat ggtttcacgg atgcgctggc
      841 tttcggtttt atcggctgat gcgctggcgg gaacagacgc cgttaccggg atcgattcat
      901 cacattcggt gatggtaaaa cccaacagat cagccacttc acgggcttcc ggggtaataa
      961 tgctggcgcg cagaaccacg gacattgcca gttcgccgcg cgcgtgtgct tcacgaatat
     1021 cattcgctgt gatgagtttt ttcacctgct tcctcctgtt tggtgagaga tgccagataa
     1081 tccaccagtt gctgcacgct ttgcgggtcg tggctgttta gctcgaaaat cggctcttcg
     1141 aagcccgtct caagcaataa ctttcgcgtt gcggcgacgt cagcatctgg catgtccgtt
     1201 ttactaatga cggcgatttg ccgtttgctg acgccaatat ccagcaaccc ggcaggcaac
     1261 cgactttccg ggtcattcgc gccgtggaca tagatcagca tgtccacatc ctgtagcgtg
     1321 gtaattaagg cgtgatacca gcggggatgg ctaaaatatt cacccggcgt atcaatatcg
     1381 ccattatcat taaattccac ggtctgtgtt tttcttgcga gggtataatt cccctgcaac
     1441 gcattaaata acgttgtttt tcctgccccg acggtaccca caaacgcaat tcgtttcatg
     1501 aatgcctcgt attaactttt ggtcatttca caaagcgtat aatttaataa gcgtcctaaa
     1561 ccactgacgg tttgtgata
//
LOCUS       DAWDRC010000739         1575 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_25164_length_1575_cov_1.940132, whole genome shotgun sequence.
ACCESSION   DAWDRC010000739 DAWDRC010000000
VERSION     DAWDRC010000739.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25164_length_1575_cov_1.940132"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          278 a          413 c          460 g          424 t
ORIGIN      
        1 acgccggaag ttctgcatct ggtgggcatt gttggggcgg ttacgctgct gctggccggt
       61 tttgcggcgc tggtacagac cgacatcaaa cgtgttctcg cttactctac catgagccag
      121 attggctaca tgttcctcgc gcttggcgtg caggcatggg atgcggcgat tttccacttg
      181 atgacccacg cgttctttaa agcgctgctg ttcctggcat ccggttccgt cattctggcc
      241 tgccatcacg aacagaacat ctttaagatg ggtggtctgc gtaaatctat tccgctggtt
      301 tatctctgct tcctggtggg cggcgcagca ctgtcggcac tgccgctggt cactgcgggc
      361 ttcttcagta aggatgagat cctcgcgggt gcgatggcga atggacatat caatctgatg
      421 gtggcaggtc tggtcggtgc atttatgacc tcgctttaca ccttccgtat gattttcatc
      481 gtcttccacg gaaaagaaca aattcacgct cacgccgtga aaggggtaac tcacagcctg
      541 ccgctgattg tgctgctgat cctttccacc ttcgttggcg cactgattgt accgccgctg
      601 cagggcgtgc ttccgcaaac gacggagctg gcgcacggca gcatgttgac cctggaaatt
      661 acctctggcg tggtcgcggt ggtcggcatt ctgctggctg cctggctatg gctgggtaaa
      721 cgtactctgg tgacctccat cgccaacagt gcgccgggcc gtctgctggg tacctggtgg
      781 tacaacgcct ggggctttga ctggctgtac gacaaagtgt tcgtcaagcc gttcctgggt
      841 attgcctggt tgctgaaacg cgatccgctg aactcaatga tgaacatccc ggctgtcctt
      901 tcccgctttg caggtaaagg tctgctgtta agcgagaacg gttatctgcg ctggtatgtg
      961 gcatccatga gcatcggtgc ggtcgtggtg ctggcactgt tgatggtact gcgttgagtt
     1021 aaggattgtg ggattgcccc tgggcatata agaacgtagg tcggataagc cgtcttcgcg
     1081 gcgcatccga cactaccccc agtccgaaga gaattttctg tcgtgagaat tcgttgaaaa
     1141 tccggtcctg acgggacttt tacaaggaat aaagatcgcc atgttactac cctggctaat
     1201 attaattccc tttatcggcg gcttcctgtg ctggcagacc gaacgctttg gcgtcaaggt
     1261 gccgcgctgg atcgcgctgg taaccatggg attgacgctg gcgctgtcgc tgcaaccgtg
     1321 gttgcagggc ggttattcac tgacgcaatc tcgccattga cggtctgtcg ttgctgatgg
     1381 tcgtgctgac cggtctgctc ggtgtgctgg cggtactctg ttcgtggaaa gagatcgaaa
     1441 aataccaggg cttcttccac ctcaacctga tgtggatcct gggcggcgtt atcggcgtgt
     1501 tccttgccat cgacatgttc ctgttcttct tcttctggga aatgatgctg gtgccgatgt
     1561 acttcctgat cgcac
//
LOCUS       DAWDRC010000740         1571 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_25239_length_1571_cov_1.844987, whole genome shotgun sequence.
ACCESSION   DAWDRC010000740 DAWDRC010000000
VERSION     DAWDRC010000740.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25239_length_1571_cov_1.844987"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    432..441
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    874..883
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1170..1179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          339 a          398 c          406 g          398 t
ORIGIN      
        1 tattattagc aaaggaagaa aaaacgggga caagcatggc aaagccgatc atcacgctca
       61 atggcctaaa aatcgtcatt atgttgggaa tgctggtcat tattctctgc ggtatccgtt
      121 ttgccgccga gatcatcgtg ccgtttattc tcgcattatt tattgctgtt attcttaacc
      181 cgctggtgca acacatggtc cgctggcgtg tgccgcgtgt actggcagtg tcaattttga
      241 tgaccatcat cgtgatggcg atggtgttgc tgttagctta tctgggttcc gcgctcaatg
      301 agttgacgcg gacgttaccg caatatcgca actctattat gacgccgctg caagcgattg
      361 aaccgttgtt gcaacgcgta gggattgatg tctcagttga ccagctggcg cattacattg
      421 atccgaatgc gnnnnnnnnn ngtttatgtt gctcgaagtg ccacaattgc ccggaaaatt
      481 tcagcaaatg atggcgcgtc cggttgaagg gatggcggcg attcagcgtg cgattgacag
      541 tgtgtctcat tatctggtgc tgaaaacagc catcagcatc atcaccggcc aggtcgcctg
      601 ggcgatgctc gccgcactcg atgttcgctt cgcttttgtc tggggattgc tggcctttgc
      661 gcttaattac atcccgaata ttggttcagt tctcgcggca atccccccta tcgctcaggt
      721 actggtgttt aatggcttct acgaagcgtt gctggtgctg gcgggatatc tgctgattaa
      781 tctggtcttc ggcaatattc tggagccgcg catcatggga cgtgggctgg ggctttccac
      841 attggtggta tttttgtcgt tgattttttg gggnnnnnnn nnnctcaata aagagtgacg
      901 gcctccgcag aggccgtcag ggttacagag ctttcaggat tgcatccacg ctggctttgg
      961 cgtcaccaaa cagcatgtgg gtgttttcct tgaagaacag cgggttttgc acaccagcat
     1021 agccagtgtt catcgaacgt ttaaagacaa tcacgttctg cgctttccac acttccagca
     1081 caggcatacc agcaatcgga ctcttcggat catcctgcgc cgccgggtta accgtatcgt
     1141 tagcaccaat caccagtacg gtatcggtan nnnnnnnnnc aaccgggtgg ataccgaaac
     1201 gcacgttaat accacgagcg cgcagtttct cggtaatttc agcgacagga tattgcgcct
     1261 gcgcgactgc catgccgtac cccggagtaa tgatcactga atgggagttt ttcagcagtt
     1321 ccgctgtctc ttctgcggtg atttcgcggt gctcgcccac ttcctgatca tcaccagtag
     1381 aagagccgtc agtgccgaaa ccacccgcaa taacgctgat aaaggaacgg ttcatcgcct
     1441 tacacataat gtaagaaagg atagcgcccg aagaaccgac cagcgcaccg gtcacaatca
     1501 gcaggtcgtt gctgagcata aagcccgcag ccgcagccgc ccagccggag tacgagttca
     1561 gcatcgacac c
//
LOCUS       DAWDRC010000741         1561 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_25418_length_1561_cov_2.784861, whole genome shotgun sequence.
ACCESSION   DAWDRC010000741 DAWDRC010000000
VERSION     DAWDRC010000741.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25418_length_1561_cov_2.784861"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          377 a          387 c          392 g          405 t
ORIGIN      
        1 ctggcaacgt tgctgatgga tgcgctaaaa cgccatcatg gcgctgcgca ggtgaccaat
       61 gttgatgtgc cgggcctggt cgtcgaactg gcaaaccatc tttctccctc gcgcctgcag
      121 gctatactgg gggatgtttg ccacattcgt gaacagttaa tgtctgtcac aggcatcaac
      181 cgcgagcttc tcatcaccga tcttttactg cgtattgagc attacctgca accgggcgtt
      241 gtgctaccgg ttcctcatct ttaagagaga catcatgttt ttagtcgact cacactgcca
      301 tctcgatggt ctggattatg aatctttgca taaggacgtg gatgacgttc tggcgaaagc
      361 cgccgcacgc gatgtgaaat tttgtctggc agtcgccaca acattaccgg gttatttaca
      421 catgcgggat ctggtaggcg aacgcgacaa cgtcgtattt tcttgtggcg tgcatccact
      481 caaccagaat gatccctacg atgtagaaga tttacgtcgt ctggcggcag aagagggcgt
      541 tgtagcgctg ggtgaaaccg ggctggatta ttattacacg ccggaaacta aagtacgtca
      601 gcaagagtcc ttcatccatc atatccagat tggtcgtgaa ctgaacaagc cggttatcgt
      661 ccacacccgt gacgcccgcg ccgatacgct ggcaattctg cgcgaagaaa aagtgacgga
      721 ttgcggtggc gtactacact gttttacaga ggacagagaa acggcgggca aattactgga
      781 tctcggattt tacatctcct tttccggcat tgtgaccttc cgtaatgcgg agcaactgcg
      841 cgatgctgcg cgttatgtcc ccctggatcg gttactggtg gaaactgact caccttacct
      901 tgcgccggta ccacatcgag gaaaagagaa tcaacctgcg atggttcgtg acgttgcaga
      961 atacatggct gtgttgaaag gtgttgccgt tgaagaactg gcgcaggtaa ccaccgataa
     1021 cttcgcccgt ctgtttcaca tcgacgcttc ccgccttcaa tccatccgtt gaatgagttt
     1081 ttttaaagct cgtaattaat ggctaaaacg agtaaagttc accgccgaaa atcgggcggt
     1141 gaataaccac gtttgaaata ttgtgacata tgttttgtca aaatgtgcaa cttctccaat
     1201 gatctgaagt tgaaacgtga tagccgtcaa acaaatcggc actgaattat tttactctgt
     1261 gtaataaata aagggcgctt agatgccctg tacacggcga ggcactcccc ccttgccacg
     1321 cgtgagaacg taaaaaaagc acccatactc aggagcactc tcaattatgt ttaagaatgc
     1381 atttgctaac ctgcaaaagg tcggtaaatc gctgatgctg ccggtatccg tactgcctat
     1441 cgcaggtatt ctgctgggcg tcggttccgc gaatttcagc tggctgcccg ccgttgtatc
     1501 gcatgttatg gcagaagcag gcggttccgt ctttgcaaac atgccactga tttttgcgat
     1561 c
//
LOCUS       DAWDRC010000742         1545 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_25679_length_1545_cov_1.904027, whole genome shotgun sequence.
ACCESSION   DAWDRC010000742 DAWDRC010000000
VERSION     DAWDRC010000742.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25679_length_1545_cov_1.904027"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    664..673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          445 a          346 c          370 g          374 t
ORIGIN      
        1 ggcgattatt catggtaaag ccgcgcctga ccagcttggc gtcaccgcag ttgatgccca
       61 tactttgaaa atccagcttg ataaaccgtt gccgtggttt gtgaatttaa ccgctaactt
      121 tgccttcttc ccggtgcaaa aagccaacgt agaaagcagt aaagagtgga cgaaacccgg
      181 aaatctgatc ggtaatggcg cttatgttct taaagagcgc gtagtcaatg aaaaactggt
      241 cgtggtaccg aatacccatt attgggataa cgccaaaacg gtgctgcaaa aagtgacctt
      301 cctgccaatt aatcaggaat ccgcagccac taagcgttat ctcgcggggg atattgatat
      361 caccgaatcc ttcccgaaaa atatgtatca gaagttgttg aaggatattc cggggcaggt
      421 ttatacgccg ccgcagctcg ggacctatta ttatgcgttt aacacgcaaa aagggccgac
      481 ggcagatcag cgcgttcgcc tggcattaag tatgacgata gatcgccgcc tgatgaccga
      541 aaaagtgttg gggacgggcg aaaagccagc gtggcatttt acaccagatg ttaccgcggg
      601 atttacgccg gaaccttcgc cgtttgaaca aatgagtcag gaagaactga atgcgcaggc
      661 aaannnnnnn nnngttatgg tccgcaaaaa ccgctgaagt tgacgctttt gtataacact
      721 tcagaaaacc atcaaaaaat tgcgattgct gtagcatcga tgtggaaaaa gaacctgggc
      781 gtagatgtta aattgcaaaa ccaggaatgg aaaacctata tcgatagccg taataccggc
      841 aattttgatg ttatccgcgc ctcgtgggta ggggattata atgacccctc cactttcctg
      901 acattattaa cgtcaacgca ttccgggaat atttcacgct ttaacaatcc ggcatatgac
      961 aaagttctgg cccaggcatc gacggaaaat accgttaaag cgcgtaatgc cgattacaac
     1021 gcggcagaaa aaatcctcat ggagcaagca cagattgcac caatttatca atataccaat
     1081 ggacgattaa tcaagccgtg gctgaaaggt tatcccatta ataaccctga agatgtggcg
     1141 tacagtcgga ctatgtatat tgtgaagcat taatgtgatg ggaactggcg ttacccttgt
     1201 ggataacgcc agtgatgtct gatttagcga ccctggtccg gcggtgtaat gttatccata
     1261 tacagcgtct ggctggggaa ggcaaagtcc gcgccgtgtg actgtacaat atcgataatc
     1321 ttcaaataaa cgtcttgctg tgcggcaagc cactcagccc ataccgtggt tttggtaaag
     1381 caataaacca taatatttaa tgacgaatca gcaaactggt tgaaataaac cagtaaggtt
     1441 tgtcgctggt cgatggccgg gtgatttttc agcatctcac gtacggcttc gacaataacg
     1501 cccacttttg ccgcatcctc ataacgtaaa ccaatggtcg tggta
//
LOCUS       DAWDRC010000743         1542 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_25732_length_1542_cov_2.365837, whole genome shotgun sequence.
ACCESSION   DAWDRC010000743 DAWDRC010000000
VERSION     DAWDRC010000743.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25732_length_1542_cov_2.365837"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          367 a          374 c          443 g          358 t
ORIGIN      
        1 tgctcttata ccctggcgat gtccacttgc cgcgaagtac gcgatggtga agaacccccg
       61 ctgctggtgc gtatcggctg gtcaattctg gttggcatta tcggtattgt tctgctggcg
      121 ctcggcggcc tgaaaccgat tcaaaccgcc attatcgccg gaggatgccc gctgttcttc
      181 gtcaacatca tggtgacgct ctcctttatt aaagacgcga aacagaactg gaaagattaa
      241 ttaaccccca aaatatcaag aggttgaaag atggatttta atttaaatga tgagcaggaa
      301 ctgtttgtcg ccggtatccg cgaactgatg gccagcgaaa actgggaggc ctattttgcc
      361 gagtgcgacc gtgacagcgt ctacccggaa cgttttgtca aagccctggc ggatatgggt
      421 atcgacagtc tgctgatccc tgaagagcac ggtggtctgg acgcggggtt tgttactctc
      481 gccgccgtgt ggatggaact gggtcgtctg ggagcgccaa cttacgtgct gtatcagtta
      541 ccgggcgggt ttaacacctt cctgcgtgaa ggtacgcaag agcagatcga caagattatg
      601 gctttccgcg gcaccggtaa gcagatgtgg aactcagcaa ttaccgaacc gggcgcaggc
      661 tccgacgtgg gtagcctgaa aacgacttat acccgtagaa atggtaagat ttatcttaat
      721 ggtagtaagt gttttattac cagcagcgcc tacaccccgt acatcgtggt gatggcgcgc
      781 gacggggctt ctccggacaa acctgtctac accgaatggt ttgttgatat gagcaaaccg
      841 ggcatcaaag tgaccaaact tgaaaagctc ggtctgcgta tggatagctg ctgtgaaatc
      901 acctttgacg acgtggaact ggacgagaaa gacatgttcg gtcgggaagg taacggcttt
      961 aaccgcgtca aagaagagtt cgaccatgaa cgtttcctgg tagccctgac caactacggt
     1021 acggcgatgt gtgcctttga agatgcggcg cgctacgcca accagcgtgt gcagtttggc
     1081 gaggctattg gtcgtttcca gttgattcag gaaaaattcg cccacatggc gatcaaatta
     1141 aactccatga aaaacatgct gtatgaagca gcgtggaaag cagacaacgg caccatcacc
     1201 tctggcgatg cagcgatgtg caaatacttc tgcgccaatg cggcatttga agtggtggat
     1261 agcgcaatgc aggtgctggg cggtgtcggg attgcgggta atcaccgcat cagccgcttc
     1321 tggcgtgacc tgcgtgtaga ccgcgtttcc ggaggatctg acgaaatgca gatcctgacg
     1381 ctgggtcgcg cggtgctgaa gcaataccgt taagtccgtg cgctgcctga tgcgacgctg
     1441 acgcgtctta tcaggcctac gggagcacga agatggccgg ataaggcgtt tacgccgcat
     1501 ccggcagtca tgcgctacat aaatttttca ggagctaata at
//
LOCUS       DAWDRC010000744         1542 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_25733_length_1542_cov_2.168796, whole genome shotgun sequence.
ACCESSION   DAWDRC010000744 DAWDRC010000000
VERSION     DAWDRC010000744.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25733_length_1542_cov_2.168796"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          357 a          410 c          386 g          389 t
ORIGIN      
        1 gcattgctac tgattttcca acatataaga agacaagccc gaacagtcgt ccgggctttt
       61 tattagaatt ggatgatcct tatccagagc atttaatcgg tgttgctttt ttccgctttt
      121 ccggccattt gtgccaggaa gtcatagcgt ttttgcaaat cagctgcagc atctttccat
      181 aactgttccg ccacttctgg ctgctgcgaa ttcagacgac ggaaacgctg ctcgtgaagt
      241 aacgtttctt ccagtgcttc tgacggcggg cgtgaatcca gcgccagcgg cagtttgcct
      301 tcatcggcac gacgaggatc gaagcgatat aacggccaga agccggtagc agtgagttgg
      361 cgcatctgat cgtggctgag tgccagatcg taaccatgct cttcacatgg gctataagcg
      421 atgatcagcg atggccccgg atacgcttcc gcttcctgaa tcgctttcac cgtctggttc
      481 agctgcgcgc cgagagaaat ctgcgccaca taaacatgac cgtacatcat catactgacg
      541 ccaagatctt tacgcgcttt acgtttgcca tgctcgccaa acttagttac tgcacccagc
      601 ggtgtcgctt tcgatgcctg acctccggtg ttggaatagc attgcgtatc cagcaccaga
      661 atgttgacgt tttccgtcaa actcaataca tgatccagac cgccaaagcc gatatcgtaa
      721 gcccagccat cgccaccaat cagccagatt gatttttcca ccagtgcatc tgcgtcacgc
      781 agcagttcat gtgcttcggc aacatcgttg agttgctggc gtaaagctgc tacctgttca
      841 cgacgaacct ctggcgtggc gtctgatttc aacgcagtca gtaattccgt cgggatttta
      901 tcggcaaatt gatcgagcag acgcagcacg cggacacggt gttgatcgac cgtcaggcgg
      961 aaaccaaggc caaattcggc gttatcttca aacagcgagt tcgcccatgc cggtccacga
     1021 ccgttggcat cggtggtata tggtgttgaa ggcaggttac cgccgtatat tgaagaacag
     1081 ccagtggcat tagcgatcaa catccggtcg ccatagagct gagtcagcaa tttaatatac
     1141 ggcgtctcgc cacaaccgga gcaagcaccg gaatattcaa acagcggcgt gattagctgc
     1201 gatgtacgaa tatcaatacg ttccagtttg ctacggtcga tttctggcag gttgaggaag
     1261 aaatcgtaat tgattttctc ttcttcgaca tgttccaggc gcgacatcat attgatggct
     1321 ttaatctctg gattctgacg gtctttcgcc gggcaaactt cgacgcacag gttacaaccg
     1381 gtgcaatctt ccggagccac ctgcaagaca tatttctgcc cgcgcatatc acgcgatttc
     1441 acatccagcg aatgcaggct ggcaggggcg ttttccatcg cttcaggcgg taccactttt
     1501 gcgcgaatag ctgagtgtgg gcaagcggcg acacagtggt ta
//
LOCUS       DAWDRC010000745         1528 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_25965_length_1528_cov_2.231500, whole genome shotgun sequence.
ACCESSION   DAWDRC010000745 DAWDRC010000000
VERSION     DAWDRC010000745.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25965_length_1528_cov_2.231500"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          353 a          393 c          390 g          392 t
ORIGIN      
        1 gctgatccac cacctaaatc aggccacccg gtgatgctgc caacttactg atttagtgta
       61 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
      121 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc
      181 tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg
      241 caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac agcccgcatt
      301 atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg cagtcggtaa
      361 cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatgggc gaacagtggg
      421 gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga
      481 agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga
      541 gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat
      601 cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc
      661 ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa
      721 aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat
      781 aagttggagt cattacctgc tttttcggtt gaaaagaaat tgattgaatt actggaaaaa
      841 caatctggtc tccatacgtc tgtggattgc agtactcctc ttacagagca gagtctgtcg
      901 agaaatgacg gtgaagacca gaccagaaga aacgctgaaa atatgttcgg agatgactga
      961 actaaccgcc atttagttgt ataacactga ctcaacattt cccgtttcat ttgccattca
     1021 ggggcggttc ctcgccccgc aaaccacact ttcccttttc ccgactggtt cagttcatcg
     1081 agtgttttca ttaacttttc actgttttta cggggctgga tttcatcaaa taatcccggc
     1141 tgcgctatac ccgatggtgt gaaatcagcc agcatgactc ctgccttcat atagcggtac
     1201 ccttcacgcc agacatggtt taaggctctg catgcggcgg caataatgtc ccggctgtcc
     1261 tgtgtgggca atggaagctt ttccacagcg gcattgctgt aacagggttc ttttactgca
     1321 aagggggatg tccgtacaaa tgtcgtcacc tgccggcaat actgacgctc cccacgtagt
     1381 ttctctgcgg cccgctctgc atactgaaca acagcctggt gcatggcatc tttgtctgtg
     1441 attcgttcac caaaactgcg actacagaca atctgctgtt ttgccggtgg tgcttcttcc
     1501 agggatatgc aggactcgcc gttgagtt
//
LOCUS       DAWDRC010000746         1526 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_25997_length_1526_cov_2.593474, whole genome shotgun sequence.
ACCESSION   DAWDRC010000746 DAWDRC010000000
VERSION     DAWDRC010000746.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25997_length_1526_cov_2.593474"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    293..302
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          314 a          371 c          347 g          484 t
ORIGIN      
        1 gcatggaatg tcggtatatt acatggcaac gtcaccataa tggcggtagg ttcgtatttt
       61 acgcccgtcc tttcctcagc gcttgcagcc gtgctgctca gcgccccgct gtcgttctcg
      121 ttctggcaag gcgcgctaat ggtctgcggc ggttccctgc tctgctggct ggcgacacgt
      181 cgtggctaaa ataattgtcg ggtctacggc ccggcagtta atcagataaa ttctgacaat
      241 attaaaaaat aaatttcaaa taccagaaag ccattttagt tacatttagt aannnnnnnn
      301 nntatctgtc tgtttaatcc acattctgcc cccgcagtta cgctttctta tctattcttc
      361 taatcagcac ttcacctgat tcatccttaa ttctaataac tggctattgc gccttacctc
      421 ctgttttgtc tttttttcat taccaatgtg tgcatgtgag gaacaatatg gcactgcaaa
      481 atgagaaaaa tagtcgttat cttttacgcg actggaaacc agaaaatccg gccttctggg
      541 aaaataaagg aaagcatatt gcacgaagaa acctctggat atcagtcagt tgtctacttc
      601 ttgccttctg tgtatggatg ctatttagcg cagttactgt taatctcaat aaaatcggtt
      661 ttaatttcac taccgatcaa ctctttttat taaccgcatt accctccgtt tctggcgcat
      721 tattgcgtgt cccctactcc tttatggtgc ctatattcgg tggacgccga tggacggttt
      781 ttagtactgc aatcctgatt attccttgcg tctggctcgg aattgccgtg aaaaatccga
      841 ataccccttt tgggatattt atcgttatcg ctttgctatg cggttttgca ggtgcgaact
      901 ttgcttcgag catgggcaat atcagtttct tctttccaaa agccaaacaa gggagcgctc
      961 ttgggattaa tggcggatta ggaaacttag gtgtaagtgt gatgcagttg gttgcaccgc
     1021 tggtcatttt tgtccccgta tttgcctttc tcggcgtcaa tggcgtaccg caggccgacg
     1081 gttcggtgat gtcgctggcg aatgccgcat ggatttgggt gccattactg gcgattgcca
     1141 cgatcgccgc ctggtcaggg atgaatgata tcgccagttc acgcgcctca attgccgacc
     1201 agctccctgt cttacaacgc ctgcatctct ggctgctgag cctgctttat cttgccacct
     1261 tcggttcgtt tatcggtttt tctgcgggtt ttgccatgct ggcaaaaacc cagttcccgg
     1321 atgtgaatat tctgcgcctg gcgttctttg gcccatttat cggtgccatc gcgcgatcgg
     1381 ttggtggtgc tatttccgat aaattcggcg gcgtgcgggt gacgttgatc aacttcattt
     1441 ttatggcgat tttcagcgcc ctgctgttcc ttaccttacc gggcacaggc tccggtaatt
     1501 tcatcgcatt ttacgccgta tttatg
//
LOCUS       DAWDRC010000747         1526 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_26002_length_1526_cov_2.368457, whole genome shotgun sequence.
ACCESSION   DAWDRC010000747 DAWDRC010000000
VERSION     DAWDRC010000747.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26002_length_1526_cov_2.368457"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          289 a          402 c          469 g          366 t
ORIGIN      
        1 gattttactc actgtatggt gggtagtcgc ggcgctgcaa ctgattagcc cgctgttttt
       61 gcctccaccg caacaggtac tggcaaaact gctcaccatt gccgggccgc agggatttat
      121 ggacgccacg ctgtggcagc atctggcagc cagcctgacg cgcattgtgc tggcgctact
      181 cgcggcggtg ttgatcggta ttccggtcgg gatcgccatg gggctaagcc cgacggtgcg
      241 cggcattctg gatccgataa tcgagcttta tcgtccggtg ccgccgctgg cttatttgcc
      301 gctgatggtg atctggtttg gtattggcga aacctcgaag atcttactga tctatctagc
      361 gatttttgcg ccggtggcga tgtcggcgct ggcgggggtg aaaagcgcgc agcaggttcg
      421 cattcgtgcc gcccagtcgc tgggtgccag ccgtgcgcag gtgctgtggt ttgtcatttt
      481 gcccggcgcg ctgccggaaa tcctcaccgg attacgcatt ggtctgggtg tgggctggtc
      541 tacgctggtg gcggcggagc tgattgccgc aacgcgcggt ttaggattta tggttcagtc
      601 agcgggtgaa tttctcgcga ctgacgtggt gctggcgggg atcgcggtga ttgcgattat
      661 cgcctttctt ttagaactgg gtctgcgcgc gttacagcgc cgcctgacgc cctggcatgg
      721 agaagtacaa tgagtgaacg tctgagcatt accccgctgg ggccgtatat tggcgcacaa
      781 attacggggg ccgacttaac gcgcccgtta agcgataatc agtttgaaca gctttaccat
      841 gcggtgctgc gccatcatgt ggtgtttctg cgcgatcaag ctattacgcc gcagcagcaa
      901 cgcgcgctgg cccagcgttt tggcgagctg catattcacc ctgtttaccc tcatgctgaa
      961 ggggttgacg agatcatcgt cctggatacc cataacgata atccgccgga taacgacaac
     1021 tggcacaccg atgtgacatt tattcaaacg ccacccgcag gggcgattct ggcggcgaaa
     1081 gagttaccct cgaccggcgg cgatacgctc tgggccagtg gtattgccgc atatgaggtg
     1141 ctctctgttc ccttccgcca gctgttgagc gggttgcgtg cggagcatga tttccgtaaa
     1201 tcgttcccgg aatacaagta ccgcaaaacc gaggctgagc atcaacgctg gcgtgaggcg
     1261 gtcgcgaaaa atccaccgtt gctacatccg gtggtgcgaa cgcatccggt gaccggtaaa
     1321 caggcgctgt ttgtgaacga aggttttacc acacgaattg ttgatgtcag cgagaaagag
     1381 agcgaagcct tgttaggttt tttgtttgcc catatcacta aaccggagtt tcaggtgcgc
     1441 tggcgctggc aaccgaatga tattgcgatt tgggataacc gcgtgactca gcactatgcc
     1501 aatgcggatt acctgccaca gcggcg
//
LOCUS       DAWDRC010000748         1525 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_26025_length_1525_cov_2.023129, whole genome shotgun sequence.
ACCESSION   DAWDRC010000748 DAWDRC010000000
VERSION     DAWDRC010000748.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26025_length_1525_cov_2.023129"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    508..517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1213..1222
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          389 a          370 c          371 g          375 t
ORIGIN      
        1 tgctgaaaat gacggtcaga ttgagttcac cgttttactg ttacaggagg ccagggcttg
       61 tctgctaccg gtccggacga gagggatacc gggaatttga atccggttaa ctgagccgga
      121 catacggtaa ataaggaatg caggcagaac gggagtcact gcaggatgag accggcacta
      181 cagcagaaaa acaggtgatt ctggtacggc tcagccacta aacacagttg gaaactgatg
      241 ataagatagt cagcggttat ataactcact gagtaaaaag atataaaggt aaggagacac
      301 tcaagagtcg ttccggtagc acactgtatc ttatcgccct gctgacagcg gcaacggtcc
      361 tgacagcctg cacgccaaag ggcagtatgg aacaacatac ccggcattac gtttatgcat
      421 cagatgacgg ttttgatcct aacttttcca cccagaaggc cgacacaaca cgaatgatgg
      481 tgcttttttt cggcagttct gggatatnnn nnnnnnntaa agcgacagga aaatcatgga
      541 gtgatgtgca acaacgcctt cagcagtttc acagccagga atttttaaac tcactccggg
      601 gcacaactca atttgcgggt accgattacc gcagcaaaga ccttacccca aaaaaatcca
      661 ggctgctggc tgacaccatt tcagcggttt atctcgatgg ttatgagagc tgacagtgac
      721 ggggaacgtt gcgggggaat aaagatattg ggggtaggct gagccccaga atatggtacc
      781 tccctgcccc tccaatattc ggtgatggcc ggacaaggag tccaggagag ggggattagt
      841 ggtggatctg cgccaggtcg tcttcagtct ttatgacgct attacggtca atgccgttct
      901 gcagcatggt acgggtaatt ttcagagtgg cttggagctc actttctgaa cgacttatta
      961 gcgtgtgctc cggtgatttg cacatgcgca aatctttcag cccaaaggag gctaatcatc
     1021 acggcgatta cgttcaaatc ccccgcgccc tcttgctgcc aattcagcat tgagtaccac
     1081 gtaacgctcc cagcgttcac gcaactgtgc gagctttagc ggtaattctt tggtgacaaa
     1141 ggcttcctgt atttcaaagt catcaagatg cccttgcaga tatgcatgta cgccttccaa
     1201 cacatcagtc agnnnnnnnn nngctctacc atacagctgg cgttttagca tcttcaggcg
     1261 atttacatga ccttcgacaa caccattact ccagcggcta ctgattgcct cacatatagc
     1321 tgcagcatca gcttccatcc ccgccgccac gcgccgaaat tctgctgaac cgctttcgtg
     1381 gactcgagtg aaccagctac ttagctgtga tttattttgg gttttcagaa tacggtagaa
     1441 ctcgagtgcc agttgctgcg ctattttcag ctccggttct ttttcacaca tcagactaat
     1501 aaatcgggat gcataatttt cttcg
//
LOCUS       DAWDRC010000749         1525 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_26022_length_1525_cov_2.228571, whole genome shotgun sequence.
ACCESSION   DAWDRC010000749 DAWDRC010000000
VERSION     DAWDRC010000749.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26022_length_1525_cov_2.228571"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          423 a          370 c          376 g          356 t
ORIGIN      
        1 atataaaata tctatatatt tgttttgtaa gaagatttca taaagacaat aacaaaaaag
       61 cacgcttcgg caagataaaa ctatcagcca taagaaaaag cgattaatag tgaataaata
      121 aaataagaag gaggaagaca aagcaaaggc cgctcacgat atagccagat aaatgacggg
      181 gatcaattgg cttacccgcg ataaaatgtt accattctgt tgcttttatg tataagaaca
      241 ggtaagccct accatgccac attcctacga ttacgatgcc atagtaatag gttccggccc
      301 cggcggcgaa ggcgctgcga tgggcctggt taagcaaggt gcgcgcgtcg cagttatcga
      361 gcgttatcaa aatgttggcg gcggttgcac ccactggggc accatcccgt cgaaagctct
      421 ccgtcacgcc gtcagccgca ttatagaatt caatcaaaac ccactataca gcgaccattc
      481 ccgactgctc cgctcttctt ttgccgatat ccttaaccat gccgataacg tgattaatca
      541 acaaacgcgc atgcgtcagg gattttacga acgtaatcac tgtgaaatat tgcaggggaa
      601 cgctcgcttt gttgatgagc atacgttggc gctggattgc ccggacggca gcgtcgaaac
      661 actaactgct gaaaaatttg ttattgcctg cggttctcgt ccatatcatc caacagatgt
      721 tgatttcacc catccacgca tttatgacag cgactcaatt cttagcatgc accacgaacc
      781 gcgccatgta cttatttacg gtgctggagt gatcggctgt gaatatgcgt cgatcttccg
      841 cggtatggat gtaaaagtgg atctgatcaa cacccgcgat cgcctgctgg catttctcga
      901 tcaagagatg tcagattctc tctcctatca cttctggaac agtggcgtag tgattcgtca
      961 caacgaagag tacgagaaga tcgaaggctg tgacgacggt gtgatcatgc atctgaagtc
     1021 gggtaaaaaa ctgaaagctg actgtctgct ctatgccaac ggtcgcaccg gtaataccga
     1081 ttcgctggcg ttacagaaca ttgggctgga aactgacagt cgcggacagc tgaaggtcaa
     1141 cagcatgtat cagaccgcac agccgcacgt ttacgcggtg ggcgacgtga ttggttatcc
     1201 gagcctggcg tcggcagcct atgaccaggg gcgcattgcc gcgcaggcgc tggtgaaagg
     1261 tgaagccaac gcacatctga ttgaagatat ccctaccggc atttacacca tcccggaaat
     1321 cagctctgta ggcaaaaccg aacagcagct gaccgcgatg aaagtgccat atgaagtggg
     1381 ccgcgcccag tttaaacatc tggcacgtgc acaaatcgtc ggcatgaacg tgggcacgct
     1441 gaaaattttg ttccatcggg aaacaaaaga gattctgggt attcactgct ttggcgagcg
     1501 cgctgccgaa attattcata tcggt
//
LOCUS       DAWDRC010000750         1515 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_26179_length_1515_cov_2.408219, whole genome shotgun sequence.
ACCESSION   DAWDRC010000750 DAWDRC010000000
VERSION     DAWDRC010000750.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26179_length_1515_cov_2.408219"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          404 a          355 c          327 g          429 t
ORIGIN      
        1 ggtggtgaaa taaattagaa aattgtttat ttaatccatt agcgataagt tttattgtta
       61 tttagccgag atatattcca ttgaaaaaag taaattggca ctgatattat atcaaatggg
      121 aataatcctt ttgtaaataa gctgaggaat gtgttaattc acatccagcg ccttataaac
      181 cgcatcaacc ggttctgagt aaaaacttac ctgaaacttg gtaaacaact ctgccggaac
      241 tgttggaata tccatagccg aggatatcgg caacagaact cgttttgcgt cgctatcgaa
      301 ggcgagttgt aaactagtgg caagatcctg cactgattat gtcagcaata ttgaacatac
      361 tgcgatagtt atcaacctca ccccctaaaa acctctttcc gatgccactc cacaaacccc
      421 tcaaaagggt aatttttacg cacctgcggc aacgtaatag ttttcccgtc aaaatcccag
      481 aataatctct caacgatccc tccgccgttt accgcatctg ataccagtac ccgcatattt
      541 tcgtccaggc caatcgatcc tttatcaaag gctttatggt gaattgcgca aagcgccagg
      601 ccattaggaa tttcacaagg accgccgtgc tgtttccatt ttatatgcgc cgcttccaac
      661 gcgacggtgg tgtcgtcgtg gcgcatatta aagccacata tcgcgcactg gtagttataa
      721 gcgcgtaata catttttgcg aaataacggg tcgcgctgtt tacggatttg ctggagatca
      781 aaacccagct cgtcagcaat ttcttcctga atactctcgg tgaagtgcgc ttcgagtatc
      841 tgttgcgcca gggtattgat gagtttttta ttgccggtta ccagggcgta gtgctgttca
      901 tcgaaaccgc ctgcaacgcg gtgttcggtc agttccttca ctggcggttg tcggctgcta
      961 cctgctgttg agcaaagctc ggcattgcgt aattgccaga atccgtcgcc ttgtaatcgc
     1021 cagaacggca tatcaggtcg gtactgtgaa cgctgtggac caaaacgttc cagtaaactg
     1081 tgcagaggtt cgtagatttc cgagccataa tcgaaaaggc gtggatgtcc attcaggtat
     1141 cccgctaata cgtatagcaa taacaatggt ttatgcggcg cgcgctgttc acccttgtgc
     1201 cagattttat gttggcaatt gcctgctgta gcgatttact ggaagccatt gatatccctg
     1261 gcagtgaaat aattgcgatc atgctcgcaa attttctgct gttcaagcct gcaagcgaag
     1321 atatattacc ttgatctcat atgttttttt acttcctgtg tgccctaatt gccatgcgat
     1381 gttgcaccga cgcaaaccac cgtttacgcc ggagcagtta aaagcgctga tggatgcgaa
     1441 taaatcaaat taatgaacgc tcacctttcg cgccaaaact gcaaaattgc ttctacaaac
     1501 ctgttagaaa taatc
//
LOCUS       DAWDRC010000751         1514 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_26209_length_1514_cov_1.842358, whole genome shotgun sequence.
ACCESSION   DAWDRC010000751 DAWDRC010000000
VERSION     DAWDRC010000751.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26209_length_1514_cov_1.842358"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          303 a          396 c          342 g          473 t
ORIGIN      
        1 acctaaggtt ttcatggtct gtaaaaagcg ataaacggtg ctttttgaca tcatgacgcg
       61 ctgcgacaac tcggttatcc ctatttcgcg ctcttcaccc agcgcctgca aaatgccaaa
      121 aacttttagc acggaagata cagaatcagg ctgtttatcc agatctgcgt tagccattta
      181 tcacctcatt gcgagtgttt tataaaaatc agaactgttt tttattataa tttcgcacca
      241 gggtggtcgc aatccatctt ttgccggtta gttacaattc tgcgacatcc accgtgaata
      301 tcagtgctag aatcataccc ctgttgatta ttcaccaaag atataaaatt cctatgccaa
      361 aagttcaggc cgacggcctg ccattgcccc agcgatacgg tgcgatatta accattgtga
      421 ttggtatttc gatggctgtc cttgacggcg caatcgccaa cgtcgccttg ccaacaatcg
      481 ctacggacct tcatgccacg ccggccagtt ccatctgggt agtgaacgcc tatcaaatcg
      541 ccattgtcat ctccctgctc tcgttttcgt ttctgggcga tatgtttggc tatcgacgta
      601 tttataaatg cggtctggtc gtttttctgt tgtcttcact gttctgcgcc ctttctgatt
      661 cgctgcaaat gctcaccctt gcgcgtgtca tacaaggttt cggcggtgca gcgttgatga
      721 gcgttaatac cgcacttatc cgcctgatct atccacaacg ttttctgggt agagggatgg
      781 gcataaactc gtttattgtt gccgtctctt ctgctgccgg gccgacaatt gctgcagcaa
      841 tcctctccat cgcatcctgg aaatggttat ttttaatcaa cgtaccgttg ggtattatcg
      901 ccctgcttct ggcgatgcgt tttctgccac ccaatggttc tcgcgccagt aaaccccgtt
      961 tcgacctgcc cagcgccgtg atgaacgcgt taaccttcgg cctgcttatt actgcattga
     1021 gtggtttcgc tcaggggcaa tcactgacat tgattggtgc ggaactggtg gtaatggttg
     1081 tcgttggtat tttctttatt cgccgccagc tttctcttcc cgtaccgctg ctaccggtgg
     1141 atttactgcg tatcccgctg ttttcacttt ctatttgcac atctgtttgc tctttctgcg
     1201 cacaaatgct ggcaatggtt tcccttccct tttacctgca aaccgtgctc gggcgtagtg
     1261 aagtcgaaac aggtttactt ctgacaccgt ggccgttagc aacgatggtg atggctccac
     1321 tggcaggcta tttgattgaa cgcgtacatg caggattgct gggtgcttta gggttattca
     1381 tcatggctgc ggggcttttt tccctggttc tgctgcccgc gtcacctgcg gatatcaata
     1441 ttatctggcc gatgatctta tgtggcgctg gatttggctt gttccagtca cccaataacc
     1501 acaccattat tacc
//
LOCUS       DAWDRC010000752         1511 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_26262_length_1511_cov_1.894231, whole genome shotgun sequence.
ACCESSION   DAWDRC010000752 DAWDRC010000000
VERSION     DAWDRC010000752.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26262_length_1511_cov_1.894231"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          338 a          388 c          421 g          364 t
ORIGIN      
        1 ttaagaacga aggtttccga cgacagcgga aatttcagta aaaagaagcc gaccttcatt
       61 tcacctcccc gatgcgttca agcacagatt gcaccatctg cattcccgtc tgacgttcgc
      121 gactgacggc ttcgttaagt cgcgtgttca gtgccggaag ctggcctaag gtatccgcaa
      181 ccatcgcttg caggctgccg tctaataaat gacggatatc aatcgccatt tctggtagcc
      241 ccagttgttg catgatcccg gcggatttat gttcgtagtt gatggcaatc gccggagtgg
      301 caaagttcat cgagataatg gcagagtgca ggcgcgtacc aacggtgagt tcacaggccc
      361 ccagaatttt gcccatttcc agatcgttga gttcatccat caccacgtgg taacgggcag
      421 gatcgctgat gtgctggcgc agattgagcg ccaccatgcg gtcgtctttg ttatagctgt
      481 cgatgcccgt acaggtggag agcgcaatca cctgataccc ttcatcgaga atgcgattga
      541 ccaccccggc aaaggctttt tcatacgctt gttgagtggt gccgagacgt ttgtcgaacg
      601 gtgccagttc gcgcagggta atggccaccg ttttctgttg tgcggcaacg tccagccagt
      661 gttgaacggc ataggtgggg gtgaagtctt tggtgtggtg atcgaccagc cacgcggtat
      721 cgacgccatg ttcaactttt gcggtggtga tattgctgcg tttcatcagt tcaaggctga
      781 ccgattcgcg caggatcagc gcgtcgcagt gaccaaaaac gtagttcgcc agttggttaa
      841 attgctcatc ctggaacggg ccgacgctgt gaccaatcat aaacagcggc tttttcgcca
      901 taaacgtgca aggcgcatgt tcaaactgcg gcacgccata gagatcgaca aaaaacgatc
      961 cgccgacctg aataatggcg tcgtaacctg acagcagtcg cacgaagtca gtgaatccct
     1021 gggcgatggc gatattacgc aacttgccag tgtcggtgac gcgtgagagc aatacctggt
     1081 gctggtagcg gcgacggagg acttttttaa cgcgcccaac cacgcccgct gcgctgttgt
     1141 gttgtttcat ttgcaggaac agcggatcgc ccattaccgg gcggttgagc agccaggaag
     1201 aactgaccgg atagcggctc atcacgtcca cttcggcatg tggattgagg atattgatgg
     1261 catcaagtaa gccgcgcaag atggcgctgt cgccacgatt gccgcaagtg tggttgccca
     1321 gaataagtaa tttcatttct tcctcataaa tttgatgcca ggtgaggctg tgtttgttgt
     1381 gttcgttgcc gttttttgcc tgatgcgacg ctaacgcgtc ttatcagacc tacaagatcc
     1441 aagcacagaa ccgtaggccg gataaggcgt tcacgccgca tccggcattc agtgcctgat
     1501 gcgacgctga c
//
LOCUS       DAWDRC010000753         1507 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_26330_length_1507_cov_2.053030, whole genome shotgun sequence.
ACCESSION   DAWDRC010000753 DAWDRC010000000
VERSION     DAWDRC010000753.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26330_length_1507_cov_2.053030"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    271..280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1183..1192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          421 a          304 c          319 g          443 t
ORIGIN      
        1 caatagctgc tctttcaaca atgaatgctg gaaattctgt ttatcaatta aacttctggc
       61 taaattgttc ttatgcttct ttttcctcct gaggtgatca cgtgatgaca aaaacttatc
      121 tgctcatggt catatctgaa gaaagcgtat gtcgcacttc gtcattaaca cctttgactg
      181 ctgatctggc aggtacagta tgtaaagtaa gccattcgtc aatagctgat gaacgccagc
      241 ccacagaacc actaccaaga cgtaccggcc nnnnnnnnnn tttcgataac ggattcattt
      301 tttcgtaaat tgtcgaacga gatataccta acaattggct cagttcaggc atccgtaaga
      361 tgcgtgaagg agtccagcag tatccggatt gatctggcat tttatccccc atagtgtggt
      421 gaactgtcgt tacagtttgt attccccggg taaatttgtc tgcgtaaaaa aatgggctga
      481 gcaaaaagac aataaataat taaaaatcaa tacgatagaa aacaacagca ttattgttat
      541 tttactttcc ccttatcgct gtcgattctc attatcattc attggtaaaa aaacagcaaa
      601 agaggccctg gtaatgactc cagtcagtag tacagtgttt tcgataaatg aaccaatggc
      661 ttttcgtgaa tttccaggga gcgtgaaaag caaatcgttt tccgggctgg tcgtttgatg
      721 tgagtacgaa gtgtcagatt gttgcgctca atgcgccggg taaatatctt gccgacaaga
      781 tgcttatcct gtggcatttc tctggtataa ctgctccggt tgtctctggt tatcatgcct
      841 gtggagaatg gcttcaggag ttccggcaac tcacgacatg tttcatcagt gcgaggacca
      901 aaagtgtaag ccagcacacc tccagatttg gtggtatacg cataccagtg ccactgctga
      961 cgagctttgt tttcgacaaa actccattgt tcatcaagtt cgcagataag cgtcacatcg
     1021 gcactggcga ctggagatgt ggttactttg cgtggtgagc gttttataaa gtgagaatga
     1081 cggtgttgat atccaccttc agcgttctgg agctatcgcg aaccccggct ccgttatgaa
     1141 ccatttcaac aatttgctct ttaatgcctg gttttcgggt ttnnnnnnnn nnttcgtaga
     1201 tgtaattcag ttgaaataca cacgtttgca ggattagcac tggaaacgct catgatctga
     1261 ggtgctgtga tcataatggt acactttata ggaattacag cggggagaat gtactgtaac
     1321 tgttgccata tggtctccag ataccaatag aataccacat taatctatcg tcagaaggca
     1381 tcaccggggc cttctgacat aatctgttaa tacgtgtact attaccctgt tcagaaaata
     1441 ttgattcaaa aatgaaaacc agttaacaga aaagcaaaat gatataatgt taaaatttta
     1501 tatagtg
//
LOCUS       DAWDRC010000754         1504 bp    DNA     linear   ENV 29-AUG-2025
DEFINITION  MAG TPA_asm: Escherichia coli isolate gSpreadC_ID_2986
            NODE_26385_length_1504_cov_2.002761, whole genome shotgun sequence.
ACCESSION   DAWDRC010000754 DAWDRC010000000
VERSION     DAWDRC010000754.1
DBLINK      BioProject: PRJNA1032156
            BioSample: SAMN37996442
            Sequence Read Archive: SRR8800866
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Escherichia coli (human gut metagenome)
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Kasmanas,J.C., Magnusdottir,S., Zhang,J., Smalla,K., Schloter,M.,
            Stadler,P.F., de Leon Ferreira de Carvalho,A.C.P. and Rocha,U.
  TITLE     Integrating comparative genomics and risk classification by
            assessing virulence, antimicrobial resistance, and plasmid spread
            in microbial communities with gSpreadComp
  JOURNAL   Gigascience 14 (2025)
   PUBMED   40569694
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Kasmanas,J.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2023) Department of Environmental Microbiology,
            Helmholtz Centre For Environmental Research - UFZ, Permoserstr. 15,
            Leipzig 04318, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.15.4
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: ILLUMINA
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26385_length_1504_cov_2.002761"
                     /isolate="gSpreadC_ID_2986"
                     /isolation_source="human gut; stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /collection_date="2019-04-05"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
BASE COUNT          405 a          369 c          308 g          422 t
ORIGIN      
        1 ttcaaccgca atcgctttca ggtttttcga acccattatt gcgccagttc ccgcaccgcc
       61 gctgtggtta cggctattca acatgccaga gagaggaaca aggttttccc cagcctgacc
      121 aatagccgtc acacaggttt ccggactggt caagcgacaa atttcttccg tcgttgcgcg
      181 cgtccctttt ccccataaga aatcggcttt ttcaaggcta actttgtcat ctttaatctt
      241 cagccatacc ggtgatttcg ctttcccttc gataataatg acgtcgtatc cagcgaattt
      301 catttgcgct gcaaaaaagc cccccatatg ggcatcgacg actaaatttc ctttggtaaa
      361 agtagaaagt gaagtgatat ttacgcgaga actacagggg gcaccagatc cagttaatgg
      421 gccggtagca aaaactaatt tattcgcttc atcgaaaggt tttgtgcctg gcggtacttc
      481 gtcatacata attttgtagc cgaagcccat gccaccgaca aaacttttaa acttactgga
      541 atcttcgagg gtaatatttc ctgtcgtgag attgactctt aatatattac ctgtccaacc
      601 gttagccatg attttttcct ttgcaagatt cacacagtaa tatctttcca ctcgataatt
      661 tttaacgccc ctgttgggca ggcgtttgcg cattcaccgc ataacacaca tttcgaggat
      721 tttttacttt cagtatttac ggtggccatc atccacgggc atgccgtagt acaggcgcta
      781 cagccaatac aacgtttatg atcgacggta atacagcctt ctttctgctg ccaggtaatc
      841 gcaccaatcg ggcagacgtt catgcattgc ggctctttgc attgacggca ggtatccgcg
      901 gtatagttga gatcgccata caggccgccg ccagagccaa ccccgttgtc gccaaagaaa
      961 taattgcgat ggattttgat acgggagaag aatgtcccta ctgagccatc gttgaagttg
     1021 gtacaagaga tttcacagcg gtggcaaccc gtgcatcgcg cacgttgggt caccagcacc
     1081 cctttgggtg tatttatcaa ccctactgtg ccgctatcaa tatcttcctg tttgcaaccc
     1141 aaaagtgaca gtaaagcagg ggcaattgtt aaaccagcaa ggccttttcc tgatatacgc
     1201 aagaattcta atcgcgttaa gccaatattt aatagtggac gatcaaccgg gttcatttat
     1261 ttcgcatcct cttcgcaaac acagtgctga caattcagca gcctgatatt aggcactcaa
     1321 ctaatacgct gctgacgctg tcctcaacac cagatatttt ccctaacccg atagtgtatg
     1381 agaaaagatt taacgctgat aaactaatcc tttatggtta ggagaaaaaa taatgtgatt
     1441 attctgggct cttaacattg atcgttatca attaaaatca caaagcaaga aattataagt
     1501 gaac
//
